LOCUS       DAOMVK010000001        33093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_175_length_33093_cov_3.492463, whole genome shotgun sequence.
ACCESSION   DAOMVK010000001 DAOMVK010000000
VERSION     DAOMVK010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 33093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33093
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175_length_33093_cov_3.492463"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="PK856_00005"
     CDS             complement(<1..694)
                     /locus_tag="PK856_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HRL00783.1"
                     /translation="MKTLLLIDGTSYLYRAFHAMAPLSSPSGEPTGALFGVLNMLKRL
                     PVDFPHDYVACVFDAKGPTFRNEMYADYKATRPPMPDELRSQAAQIQELVAQMGWPVL
                     SVSGVEADDVIGSLAVQAEAQGCKVIISTGDKDMAQLVTPHIMCVNTMNGDVLDEAGV
                     QDKFGVRPDQIVDYLTLIGDKVDNVPGVDKVGPKTAVKWLEQYDSLDNVMANAGDIKG
                     KVGEHLQAALDHL"
     gene            828..1556
                     /locus_tag="PK856_00010"
     CDS             828..1556
                     /locus_tag="PK856_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRL00784.1"
                     /translation="MSLYEKIPQPMLSESTRIDLRARESYHVLKIISEFVESGEELQA
                     IQPAVSIFGSARTQPDDPMYQKCEHLARLLSDGGFSVLSGGGPGMMEAANKGAYYGKS
                     PSVGLNIVLPHEQKPNDYQDLSVKFHHFFPRKVMFVKHAIAFVGMPGGIGTLDELFES
                     LTLVQTGKIKRIPIILCGRYFWSGLLEWMQNVLVAEKVISPHDMDLVQIVDEPEEIVR
                     IIFQHYETSGMNQNELTYQQQLSL"
     gene            1816..3048
                     /locus_tag="PK856_00015"
     CDS             1816..3048
                     /locus_tag="PK856_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HRL00785.1"
                     /translation="MKTQVAIIGAGPSGLLLGQILSKSGIDNVILERKDGDYVLGRIR
                     AGVLESVTVEGLKRAGVTKNLEAEGIPHDGCKMVFNGKAYRIDFEALVGRHVTVYGQT
                     EVTRDLMQARAEIGATTVYDASNVALHDVDGDAPYVTYEKDGETHRLDCDFIAGCDGF
                     HGVSRQTIPADVLKTYERVYPFGWLGMLADVPPCHEELIYGKSEQGFYLCSQRSCHEE
                     LIYGKSEQGFYLCSQRSKTRSRYYLQVALTENVEDWSDERFWNELKSRMLPEDAKNVV
                     MGTSLEKSIAPLRSFVTEPMRYKKLFLAGDAAHIVPPTGAKGLNLAASDVMYLADAFE
                     DYYHNQSEAGIDGYSARCLKRVWGAERFSWYMTKMLHTFPDDEMWDIKMKEAEFNQLL
                     NSEYGAKLLAENYTGLPY"
     gene            3169..3993
                     /locus_tag="PK856_00020"
     CDS             3169..3993
                     /locus_tag="PK856_00020"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRL00786.1"
                     /translation="MNLPEALHLGPLTLSTTVVMWLVVLGFASWLGARIAKTHNVAFD
                     GWFYGVLLFAIVVGRAVFVWQYWAEYAGVLGKIIDVRDGGVHVGAALMAAGLAALVLL
                     VCKKPLRKAVLASLLTVYVGAACIHQFGPNAAHQGLRLPNMALQTLAGETRSLQDLQG
                     KPVVVNLWATWCPPCRREMPALMQFAEKNPQVVVVLVNQGEEAATVAAYLQQEKLSSD
                     AVWLDPRSDMGVFVEQQALPTTLFFDALGRLQEVRVGELSEATLQQKVSTLQAAQP"
     gene            4019..4702
                     /locus_tag="PK856_00025"
     CDS             4019..4702
                     /locus_tag="PK856_00025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRL00787.1"
                     /translation="MTESLDARLLWLAEQVRPNVFFIDIGSDHARLPIYLVQSGRITQ
                     AVASDIATGAVAQGRTHVAEQGLEAQIALRLGNGLQGMDMPAVADIAICGMGADSICA
                     IISDQPRVKQGQTRLLLQAMTDTPVLRQYLAAEGFGIEAEDCVYAEGRYYQCMVAVYT
                     GAVRELDVAAAELGELNLRQRSPAFLQSVREREQRLQKWLPIKANAGQDTALDTSLLA
                     VYAQVLREH"
     gene            4841..5236
                     /locus_tag="PK856_00030"
     CDS             4841..5236
                     /locus_tag="PK856_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00788.1"
                     /translation="MQISYRTALLSSLLGLALVVAMQAYSGLTCYEQTWDVLLTNIGI
                     FVMVPLIPAFIALFTRNPLSALGGFLAFLPWLIYAYYVDCMTPHTGSGGASLIYVVVF
                     LYGAASCLLGVLFVAVLMWLMQAKVVKGR"
     gene            complement(5377..6090)
                     /locus_tag="PK856_00035"
     CDS             complement(5377..6090)
                     /locus_tag="PK856_00035"
                     /inference="COORDINATES: protein motif:HMM:NF014970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MutH/Sau3AI family endonuclease"
                     /protein_id="HRL00789.1"
                     /translation="MQTIYTNKELALQRAKESMGKSFGDINQIIIDRGGKDGLSNTKG
                     GLGNILQEGWFNIPANSFAEADLEEAGLEIKVTGYKPDVRNGGFKAKERLVCNIINYE
                     TENLDDFYQSSFWQKNKSILLFAYQYQIGVHRSEYTIVDAQLIDLSSPEFADDLQVIQ
                     DDWRIITDKIRQGRAHQLSESDTRFLGACTKGANSSSLRKQLYSTEWAMQRAYSLKIQ
                     YLSVILKQRFELSDASLAI"
     gene            6192..7460
                     /gene="dcm"
                     /locus_tag="PK856_00040"
     CDS             6192..7460
                     /gene="dcm"
                     /locus_tag="PK856_00040"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HRL00790.1"
                     /translation="MSSQKIIRVVELFAGVGGFRLGLERTDKQRFQTIWANQWEPNKK
                     SQHAYSCYIKHFGSSPNHVNEDIAFAKFSVPEHDLLVGGFPCQDYSVARTGAQGIEGH
                     KGVLWWEIRDIVQTHRPEYILLENVDRLLKSPATQRGRDFGVMLRCLNDLGYVVEWRV
                     INAADYGFAQRRRRIFIFATRQDSLMLKSLQVDDESLKEWLLHDGLFAQAFPVEPEIH
                     TKKITTSLIDSKHFSELADVSERFKVELHNAGVMCNGKFVSCEVMPISKPFIPLKNIL
                     HNAFAVKHKEQQPFEVEKRYFLSDDTEKWRYLKGAKRIPRIKPNGEPYFFAEGAMCFP
                     DNLEASARTMLTSESSINRSSHVVADLLNGQLRLLTPIECERINGFDDNWTNVEGVPH
                     KFRYFAMGNALVVPVVEVLSKTLLQRIDVN"
     gene            complement(7540..>8006)
                     /locus_tag="PK856_00045"
     CDS             complement(7540..>8006)
                     /locus_tag="PK856_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRL00791.1"
                     /translation="SLWTRNPELGTIAACKDIGATLVAFSPVARGFLGNVLHDTTTLP
                     EKDIRLGMPRFQGEHFQHNLTLLNAFAALAKQAGCTPGQLALYWVLAQADHIVAIPGT
                     TSLQHLADNIATPTLNITTNILAQASDLINQHTVSGPRYPAATQLEIDTEEF"
     assembly_gap    8007..8035
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8036..8533)
                     /locus_tag="PK856_00050"
     CDS             complement(<8036..8533)
                     /locus_tag="PK856_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRL00792.1"
                     /translation="MFAPRTLAATPVNPIGLGCMNLSHAYGTPPSTQDAQTLLFKALD
                     LGYNHLDTATLYGFGNNETLLGNTLKGQRQQFFLASKCGMGGVNGKRVIDGHPKTLRR
                     QIDESLQRLQTDVIDLYYLHRYDKNIPIAESVGEMGRMVEAGKVRHLGLSEVSAATLK
                     LAHAEH"
     gene            complement(8623..9579)
                     /locus_tag="PK856_00055"
     CDS             complement(8623..9579)
                     /locus_tag="PK856_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRL00793.1"
                     /translation="MTQKVRIALVGTGAFGIKHLDGLKNIDNAEVTFVVGSSQDKAQV
                     VADQYGIAHAVGSLDEALASGQVDAVILCTPTQMHASQTLACLKAGKHVQVEIPLADA
                     LGDAEEVLALQQATGLVAMVGHTRRFNPSHQYLHNKITAGELNIQQMDVQTYFFRRKN
                     INAKGEARSWTDHLLWHHAAHTVDLFRYQAGAPIVKANAVQGPIHPELGIALDMSIQL
                     QAANGAICTLSLSFNNDGPIGTFFRYICDNGTYIARYDDLVTGKEEPVDLTGVTVSDN
                     GIELQDREFVAAILEGREPNSSVQSVIDCYRTLQALELQLQA"
     gene            complement(9595..10440)
                     /locus_tag="PK856_00060"
     CDS             complement(9595..10440)
                     /locus_tag="PK856_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol dioxygenase subunit beta"
                     /protein_id="HRL00794.1"
                     /translation="MAKITASVYTSHVPAIGAAWDLGKKDEPYWKKVFDGYEFSKQWH
                     KDNKPDVVFLVYNDHANNFSLELIPTFALGTAAHFETCDEGWGPRPVPDIEGAPDLAA
                     HIAHSVITQDFDLTLCNKMKLDHGLTVPMTLMFGDVEKWPVKVIPLHVNVVQYPIPSG
                     RRCYELGKAIRRAIESYDEDVNVHIWGTGGMSHQLQGPRAGLINPEWDNQFLDDLINA
                     PAELAEKNYVEYLREAGSEGVELVMWLIARGAMDEDQTPKVTHRFYHVPASNTAVGHL
                     ILENQ"
     gene            complement(10440..10850)
                     /gene="ligA"
                     /locus_tag="PK856_00065"
     CDS             complement(10440..10850)
                     /gene="ligA"
                     /locus_tag="PK856_00065"
                     /EC_number="1.13.11.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018579 - protocatechuate 4,5-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 4,5-dioxygenase subunit alpha"
                     /protein_id="HRL00795.1"
                     /translation="MAIEKPYKDIPGTIVFDSDQSRKGYHLNQFCMSLMSAENRARFK
                     ADERAYLDEWAMSEEQKQAVLDRDLNRCMDLGGNIYFLAKIGATDGHSFQQMAGSMTG
                     MSEEAYRDMMLAGGRQIDGNRYLHEWEDKQKEDK"
     gene            complement(10881..11807)
                     /locus_tag="PK856_00070"
     CDS             complement(10881..11807)
                     /locus_tag="PK856_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRL00796.1"
                     /translation="MSTTVFEKTPGWLDWYQGPSKPTLKLPAGAVDAHCHVFGPGNEF
                     PYAPERKYTPCDASKAQLFALRDHLGFARNVIVQATCHGKDNRALVDALLASDGKARG
                     VATVGVDITTEEIQTLHDAGVRGVRFNFVKRLVDFMPKADLEAIAAKIAPFGWHIVIY
                     FEAPDLPELWDFFTNLPTTVVVDHMGRPDVSKPVDGEEFNLFVKLMAEHPNIWSKLSC
                     PERLSMTGPKALDGETRPYTDVAPFARRLMELFPERVLWGTDWPHPNLKDHMPDDGLL
                     VEYIAQIAPTEAERQQLLVNNPMRLYWPEEVA"
     gene            complement(11824..12519)
                     /gene="ligK"
                     /locus_tag="PK856_00075"
     CDS             complement(11824..12519)
                     /gene="ligK"
                     /locus_tag="PK856_00075"
                     /EC_number="4.1.1.112"
                     /EC_number="4.1.3.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HRL00797.1"
                     /translation="MEVTLNQLGVVKRNIERADAAAVAKLSQFGVATIHEAMGRVGLM
                     KPYMRPVYSGAKICGTAVTILLQPGDNWMMHVAAEQLQAGDVAIAACTTDNADGFFGD
                     LLATSFKARGAKGLIIDGGVRDVDELEKMGFPVFSKAIHAKGTIKATLGSVNIPVICA
                     GQLVNPGDVVIADVDGVVVVPRERAQEVADAAEKRESFEGEKRARFEAGELGLDMYKM
                     REPLEKAGLKYID"
     gene            complement(12522..13553)
                     /locus_tag="PK856_00080"
     CDS             complement(12522..13553)
                     /locus_tag="PK856_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRL00798.1"
                     /translation="MSLIIDCHGHYTTAPKALADWREKQIATLNNPSQAPKVADLKIS
                     DDEIRETLEANQLKFMNERGSDLTIFSPRASFMAHHIGDYQVSATWAAICNELCYRVA
                     QLYPDRFVPAAMLPQSPGVPVETCVAELERCVNEYGNVGINLNPDPSGGHWTSPPLTD
                     KYWYPIYEKMVEYDIPAMVHVSTSCNPAFHTTGAHYLAADTTAFMQCLTGDLFKDFPS
                     LRFLIPHGGGAVPYHWGRFRGLAMILKKPEIEEHLMNNIFFDTCVYHQPGIDLLLDVV
                     PNKNILFASEMIGAVRDIDPRTGFYFDDTKKYIDAAKISAQDKHNIYEANTRRVFPRL
                     DAQLKAKGL"
     gene            complement(13873..14985)
                     /locus_tag="PK856_00085"
     CDS             complement(13873..14985)
                     /locus_tag="PK856_00085"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HRL00799.1"
                     /translation="MAKQTAIPCMLMRGGTSKGPFFYAKDLPTDIAARDAVLLAAMGS
                     PDKRQIDGLGGAHSLTSKVGIVDVCAGDEADLSFLFAQLQPEKDTVDTTPNCGNMLAA
                     VVPFALEKGLITAQGDTTTVKVLTLNTGMVAEITVQTPNGEIDYEGDARIDGAPGHSA
                     PIKINFLDTAGSVAGSLLPTGNVVDVFHIDGVGDLQATCIDNGMPMVLVRANDMHRTA
                     YESVADLNQDADLKAKIEQLRLQAALKMGLGDVSNKNYPKMCLLNSPINGGAIHTRCY
                     IPHVCHDAIGVLAAVTVATACVLPGSVADGLAVASKKEGNSSLMSIEHPSGEFSVELT
                     TAADDATNVLSAALLRTARLIMRGEVMVASHLGAQA"
     gene            15146..16048
                     /locus_tag="PK856_00090"
     CDS             15146..16048
                     /locus_tag="PK856_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRL00800.1"
                     /translation="MNTVELKQLKYFMHVAELGSYTRAAEVIDVAQPVLSRQIRQLEI
                     ELRQNLLIRHGRGVVLTDSGRTLLKHVNIIMDQIDEAYEDLSLSDGKLAGHITIGLPP
                     TVAKLIAIDLVRAFKQRLPLAHLTIEEGLTVHIEENLGLGRMDIGLLNNPTFSPNIDT
                     KLIIEEELYLIARKGYFPHQPECMDLKELATIPLIMPTLPNPFRVLVQAQMAEIGLKP
                     QIDWEINSVGTIVKLVNEGVGCAVLSKYCLGLVRDGDNMEAIRIQNPALINRLYVGYS
                     AKRATTRLQKEMLQLVEEVCQQYF"
     gene            16343..17746
                     /locus_tag="PK856_00095"
     CDS             16343..17746
                     /locus_tag="PK856_00095"
                     /inference="COORDINATES: protein motif:HMM:NF015532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015802 - basic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OprD family porin"
                     /protein_id="HRL00801.1"
                     /translation="MKRHLMTGLPLAALCVLPTLAFADDASTENTANATNADSVWASP
                     FFDDAKLSVLNRNYYFNRDFRHGGANPSGSNQFKPVGERNGYRSEWAHGIVVDVQSGY
                     TEGVVGFGVDAQALVGVRLDSSGARTGTGLVPVGKDGEPAHTWGEVSGAVKAKVGKTV
                     LKYGTQMPNVPVLSVHNARLLPATVNGVSLESNDLDKLNLQAAWFTHTNAIDSTNHDD
                     RFNTDYGFFLALVAKRAGYVGGTYKWSPDTQASVYVGHLKDVWNQYYAQGNHTWRFDN
                     NTFVKLNGVGYWNRDTGKSLAGSIDSKLLRTNVTYGNATHAITAGYQQVFGDEPLDYI
                     GFRTIGGNVNFANIGNVSPFAEANEKSWQLRYDLDFKELGVPGLSFTGRYIKGFDMDN
                     KNSSNQVYKNALKGYTYRDGDSHWERDFELKYVFQSGAAKGLSLRLRQATLRASTGYR
                     YNNMDEVRIIAEYPWSL"
     gene            17756..18187
                     /locus_tag="PK856_00100"
     CDS             17756..18187
                     /locus_tag="PK856_00100"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HRL00802.1"
                     /translation="MQTVYVLNGPNLNLLGVREPEIYGRDTLADIEALCQQIAEQFGW
                     QLRFLQSNYEGALVEWVHEARAAGAAVVMNPAGCSFYSIPLLDALSTLETPYIEVHLS
                     NIHARDADHQHSILSKKARGVICGFGAHGYELALQALKQLL"
     gene            complement(18321..19130)
                     /locus_tag="PK856_00105"
     CDS             complement(18321..19130)
                     /locus_tag="PK856_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HRL00803.1"
                     /translation="MRNTEIVHEHTQEIIRYDDFIAGLAKGLQVLECFGYDRQRLNVT
                     QTAERTGLTRTAARRHLKTLKYLGYLDSDGTFYWLTHRVLKFSGAYLSSAHLPKIAQP
                     LLHSLSSQTGMCHSVVILDNHEVITVACSYLASAEKNRVLPYGIHNGNRIAAHTASNG
                     KIILAYMQDQELDTWLEHYPLTRSTKYTVTDSDTFKQQLHDIRDQGWCLAVEEHEVSF
                     IGFSVPIFDGSGRVVAGLNTVARSEQYTVEQVTNRCLVPMQELAKQLRMLL"
     gene            19396..20133
                     /locus_tag="PK856_00110"
     CDS             19396..20133
                     /locus_tag="PK856_00110"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HRL00804.1"
                     /translation="MRLNQKTAIVTGAGSGMGQAEAIGFAKEGAKVVVADVNLATAEA
                     TRDAIVAAGGEAIAVAVDVTYLADLQNLVDTTLATYGRIDVLVNNAGIFDYYTNSLDT
                     SEDLWDKIFAINVKSMFQLSNLVLPHMQAQKQGAIINICSIAGLVANMGGAAYTASKH
                     AVAGYTKHLAAVYGKDGIKINAICPGTIRTAMTAEMLKTRPTDKIPLDRFGEVGEVVD
                     LAVFLASDEARFMNGALVPIDGGFTIV"
     gene            20452..21714
                     /locus_tag="PK856_00115"
     CDS             20452..21714
                     /locus_tag="PK856_00115"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid/H+ symport family MFS transporter"
                     /protein_id="HRL00805.1"
                     /translation="MVIVDGLDVAVMGFIAPSLKEQWGISNSDLAPVISAALFGLTIG
                     AMVAGPLADKFGRKKVLLVCVWGFGLFTLAGAMADSVSHLVVLRFIAGLFMGGVMPQC
                     VTLATEYSPKRITSFLITVVICGFSLGAAMGGFLASWLIPAFGWQSMLVVAGVVPCLL
                     ALVLTWKLPESTGFMILKKQPTAKIRRYIEKIATQPLPQDVQFVLPAALAKADKPMQT
                     VLNSHYLIGTLCLWGCYFFGLFAVYLLGSWLPLITKEAGFSTSEASIIAAVFQLGGPL
                     GCITVGYLMDKFGNHRVLALCFVAVAASLAAIVMASGSFALFCVVCFLIGATLNGGNT
                     GLNALSSVFYPVSARATGNSWMHGVGRVGAILSAFAGAHLLNLGWHIDRIFLMLMIPA
                     LLVMACIVIKGMRYGKTQPEVQLQNKTV"
     gene            21733..22539
                     /locus_tag="PK856_00120"
     CDS             21733..22539
                     /locus_tag="PK856_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRL00806.1"
                     /translation="MTIQLNGASRVYYVVGDPIAQVKSPQGVTELMQQQGVNAACIPA
                     HVAPEQLAAFAVGVKPIQNIDGVIVTVPHKMAYKNLCDSVSPEAAFLGSVNTVRKTAT
                     GWHGDMFDGQGFVAALLKKGGVLQDKKVLLAGAGGAGGAIAYSLVQAGVAELAIAERD
                     AARRDDLIARLQGLGLGKISVGSDDPSGFDVVLNATPCGMKADDPLPFQVDKLQAAVF
                     VGDVITAPEVTPFIAAGRALGCPSMLGMDMFAEVRDLMVQFLLQGEEVQA"
     gene            22536..23363
                     /locus_tag="PK856_00125"
     CDS             22536..23363
                     /locus_tag="PK856_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRL00807.1"
                     /translation="MSRSYSLSFLTVQEASPVEAVEIAAACGYDYVGLRILPAAASGE
                     AAYPLLHDANLLREVQAALRDTGVKVADVEIVRLKDSYRRADFQPFVERAAALGAAHV
                     LVAGDDTDAARLQDSYTDFCHLAAEYGLSADLEFMPWTAVKNLRQARAMVEQSGAANA
                     GVLIDSLHFARSDSSLAEVADLPSDLIHYIQLCDAYAQYDDSDEGLIAVARGARLLPG
                     SGELDLLSLAQLVDASKPIGLEIPNAELVQHFSPKQRAQMAIDHAQKLLATAMVGGE"
     gene            23372..25072
                     /locus_tag="PK856_00130"
     CDS             23372..25072
                     /locus_tag="PK856_00130"
                     /inference="COORDINATES: protein motif:HMM:NF004789.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRL00808.1"
                     /translation="MHTARDTDVLIVGSGASGLAAAIAAAHAGLKVVVAEKANVVGGT
                     SAFSGGWLWIPHAPHTKGQNTGDTAADVEAYLQAVLGERYDDTMMQQYLQRAPEMLAF
                     FENHTAVKFLPGSAVPDFFGELAGAKAGWRSVVAAPYDGRGLGKNLAMLRRPIPETTL
                     WGMGIASGADMKHFLHSFKSWSSLYYVGKRVSKHGRDVLLHGRSQQIVNGNALVARLL
                     ESALRLNVSFVLNARVSDIGEADEAGARSVVVDTPQGQTHIHARRAVVLAAGGFPHDV
                     VRQSQLFPHVKNGTPHHSAAPTTNTGDGLNLGERLGGEVDSSQAAAGAWSPVSLVPQA
                     DGTWGRFPHLVERGKPGLIAVRANGLRFTNEGGPYHAFVKEMLAATPEGERAVSWLIC
                     DHTFIRRYGLGAVKPFPLSLKPWLANGYLKRGNTLAELAHACGIDVNGFEATLREYNR
                     HAEQGQDPLFGRGSNLYQRVAGDASVGPNPCVAPIVTAPFYAVEIVPGSLGTFAGLKT
                     NVHAQVLDSTGAVISGLYAVGNDMNSVMGGHYPSGGITLGPGMTFGYVAARHIAGKQD
                     "
     gene            25082..>25380
                     /locus_tag="PK856_00135"
     CDS             25082..>25380
                     /locus_tag="PK856_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRL00809.1"
                     /translation="MEQKTVDLLVIGGGAAGMTAALTGRMEGLDVLLCEKAKQVGGTT
                     ATSGGTTWVPGTHLSVQTGVPDKVEDARAFLKSVVGERGGEHLREAFLRSGPDMVA"
     assembly_gap    25381..25390
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <25391..26188
                     /locus_tag="PK856_00140"
     CDS             <25391..26188
                     /locus_tag="PK856_00140"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRL00810.1"
                     /translation="KFAAALAHPDYVKNQPGEAFGGRALAPVAFDAKVLGDDFDRVLP
                     PRPEFMGLGGMMVNRNELSALLRPVSSVGNVKTTLKVVLPYFKDRLKYKRGTRLVMGN
                     ALVGRLLYSLKQYQTEIWYEAPLQELLLEEGRVIGAIVDTVNGRQRIIARKGVVLATG
                     GVAWNPALRQQYFPVGTRDYSLAPQLSTGDGLSNGLKVGAKLDNEDHPVLWFPCSTFK
                     KPNGQMAVWPHIILDRAKPGLIAVNASGKRFVNETDSYHDFCVGQLT"
     gene            26167..26733
                     /locus_tag="PK856_00145"
     CDS             26167..26733
                     /locus_tag="PK856_00145"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRL00811.1"
                     /translation="MRGAAYLICDDAFIHKYGLGLIMPGAQKLKHYLKHGYVVQAATV
                     RELAGKIGVDAHELEQSVVRNNAYAITGEDLEFGRGTGKMNRFNGDAAIGPNPCIGPI
                     LTAPFYALAVKPIDCASSVGFAGDEYGRVLNQQGVAIHGLYACGNDLASIFKGTYPGP
                     GTTIGPGMVFGWRIAKHAAGKLAHESGL"
     gene            26786..27400
                     /locus_tag="PK856_00150"
     CDS             26786..27400
                     /locus_tag="PK856_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HRL00812.1"
                     /translation="MYYYELATLDIQMGKAADVAAGVEKFVAEALTGRLLGVWFSDIG
                     ALNQVLVLREFKTLDELNQERARFAQAANPFYAQEWLEHVSVDSYQGFPFLPEVETGA
                     FGPAYEIRTYHMKHGGLPHVHAAWEAALPERTKLSKLTVVMSSLDGEPRIVNIWPYDS
                     VNQRSQVRADAVAQGIWPPKGGPQWLTNDMASLIALPTKNSPLQ"
     gene            27590..29344
                     /locus_tag="PK856_00155"
     CDS             27590..29344
                     /locus_tag="PK856_00155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRL00813.1"
                     /translation="MSLQNKTLNVDAVCDLVVVGSGAGGLSTAITATKRGLNVIVVEK
                     EVVYGGTTAFSGGVLWVPNNPHAQKQGIADSREAAMRYLQNEAGEHFDAEAAAVFFDN
                     APRMVEFFERETTVKFVPTLYPDYHPDVDGGVDIGRSILAAPFDIRELGDEMARLRPP
                     LKTITFIGMMFNSSNADLKHFFNVTKSPTSFVYVAKRLATHVKELTLYKRGIQVTSGN
                     ALAARLAKSAFDLNIPIFTETAAKELLTENGRVVGLQVKTADGSVRKIQAKHGVVLAS
                     GGFSHDRARLKKLYPHVASGGEHFSPVPTGNTGDGARLAESVGATVSSDYADNSAWMP
                     VSRVPMKGGEFGVFPHLLDRYKPGVIGVLKNGQRFCNESNSYHDVGAALWRACAGEAE
                     TAMWLVCDAKTLAKYGLGYVKPAPLPHGKLIQQGYLFKGASLDELAQKTGIDAQGLRD
                     TIADYNVHAAHGEDPAYGRGTSTFNRYLADPEHQPNPCVAPVAQGPFYAVKVYMGDLG
                     TFNGIETTPVGEVLDADKQVIAGLYSVGNDRKSVMGGNYPGAGITLGPIMTYGYVTAN
                     VIADQAQVHDLQAASAAV"
     gene            29363..29695
                     /locus_tag="PK856_00160"
     CDS             29363..29695
                     /locus_tag="PK856_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HRL00814.1"
                     /translation="MNEQDYPIVDLRFYSIAPRGMAEFLQHFNQLAMPLLMKHLGTPV
                     GFYTTTVGTLNQFVHIWEYESLADYERRSNARDADPEFVTYLQATKGLVVAQHNQIIK
                     KVAMNTQK"
     gene            29720..31075
                     /locus_tag="PK856_00165"
     CDS             29720..31075
                     /locus_tag="PK856_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid/H+ symport family MFS transporter"
                     /protein_id="HRL00815.1"
                     /translation="MKNNNVLDVREVINGPKISGFQKLVVFFCFAIIAIDGFDVVVMG
                     LAAPQIMQEWGVSAKELGPVLSAALFGLSIGALVAGPLADKFGRKAVLVASVLFFGAF
                     TVITAFSQDITHLVVYRFLTGLGLGAAAPNAATLSAEYAPERRRAFFVTLAYCGFSLG
                     AAAGGFVAAWMIPAFGWRSVILLGGVLPLVLLPFMIWKMPESLSFLVAKNAAAAKIRT
                     IINKISPQLANENSHYTVGEPVAKAQSALGLVVSKPYFLGTMMLWLAYFTVLFLIYLC
                     SSWLPTIIKMNDFSISQAATVTSVFQIGGPLGCLFFGWAMDKFKPHKVLAIAMFVGAI
                     ATYALGQFGHNLVLLSVCAWIVGFCFNGGSVGMSALATNFYPTQARATGASWMNGIGR
                     FGAILSAFAGAHMISQGYSLQTMFTLLVIPALLAGLAVLVKGWKARTVAAPALDTAVA
                     R"
     gene            31243..32202
                     /locus_tag="PK856_00170"
     CDS             31243..32202
                     /locus_tag="PK856_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRL00816.1"
                     /translation="MWQSLLEATQFSLSVTLPNLLLLGLGLWLRYRRQVDNAFCAQAS
                     NLVFNWSLPLLMFFSIVGSDADYRAQVDMVIAGALCALVLFFGSEWVAKKIVPEVRDR
                     GVFVQGVYRANTGIVGLAFCANAYGAEGIAVGAVMTGAMTFLYNILAVITLTRSLNPD
                     TQIPWAAMLKQMVKNPLIVSIVVAIGWKSLDVPLHPVFDQTGHFLAAMALPLALICAG
                     AAFDLKSVLKVSGVSQWASVGRLLVAPIVAVIIGKAMGLEGIQFGVLFMMSATPVAAA
                     SYVMVKAMGGNDVAAANIVGITTFGAMFVAALGLGLMKPLGWL"
     gene            32426..>33093
                     /locus_tag="PK856_00175"
     CDS             32426..>33093
                     /locus_tag="PK856_00175"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRL00817.1"
                     /translation="MNTLMYLACMLIWGLNFIAVKIQGTENPLEVSLLYRSAIAFGLF
                     FGLYWWKIRPKRQALAPKLLGTVMGFGLCSFALSYLLLYYATIYSSAAMVTLIFSLKV
                     MVTPLLIGRVFKTPISPRVYVGGVFGIVGVAVIVLPNLHALSHSFLIGLGLAVAGTLV
                     TAVGDTLSYYNNQHHVEPITANSIGMLTALLVLAALTFATGKPLHLPASVHYWAGLLY
                     LALLG"
BASE COUNT         7616 a         7710 c         9183 g         8545 t
ORIGIN      
        1 gcaaatggtc taatgcggcc tgtaaatgct cgcccacctt gcctttgatg tcgcccgcat
       61 tggccatgac attgtccaag ctgtcgtatt gttccaacca tttcaccgcg gttttcgggc
      121 ctactttgtc cacacctggc acattatcga ctttgtcgcc gatcaaggtt aaatagtcca
      181 cgatttggtc tgggcgcacg ccaaatttgt cttgtacacc cgcttcgtct aacacatcgc
      241 cattcatggt gttcacgcac atgatgtgtg gcgtcaccaa ttgcgccatg tctttgtcgc
      301 ccgttgaaat gatgactttg cagccttgtg cctcggcctg tacggccaaa gagcctatca
      361 catcatcggc ctccacgccg ctcacgctca acacaggcca acccatttgc gccactaatt
      421 cttgaatttg cgcggcttgg ctgcgcaatt cgtctggcat tggtgggcgc gtggctttgt
      481 aatcggcata catctcattg cggaaggtcg ggcctttggc atcgaacacg caggcgacgt
      541 aatcgtgcgg aaaatccaca ggcaaacgct tgagcatgtt gagcacacca aacaaagcgc
      601 ctgttggttc gccgctgggc gaagacaagg gagccatcgc atggaaggcg cggtagagat
      661 aagaggtgcc gtcgattaaa agcaaggttt tcatggtgtc gtgtaccgag ttgggcgagc
      721 ctgaattgta gcctaaagcg cgcaaacaag ggcaaagctt tgccgagcat gacattcttg
      781 gggcgagtgt gttagcctga agaggcttta gaactaggga gatgaccatg agtttgtatg
      841 aaaaaatacc ccaaccgatg ttgagtgaat ccactcgcat agacttgcgc gcacgcgagt
      901 cgtatcacgt gttgaaaatc atctctgaat ttgtggaatc gggcgaggaa ttgcaggcga
      961 tacaacccgc ggtgagcata tttggctcgg cgcgcaccca gcctgacgac ccgatgtacc
     1021 aaaaatgtga acaccttgcc cgcttgctgt cagatggcgg tttttcggtg ttgtcaggtg
     1081 gtgggccagg catgatggag gcggccaaca aaggtgctta ttatggcaaa agcccatccg
     1141 tcggcctgaa cattgtgttg ccacacgagc aaaaaccgaa cgactaccaa gatttgtcgg
     1201 tgaagtttca ccattttttc ccgcgcaaag tgatgtttgt caaacacgca attgcctttg
     1261 tcggcatgcc aggcggcatc ggcactttgg acgagttgtt tgaaagcctt accttggtgc
     1321 aaacgggcaa aatcaagcgc attccgatta ttttgtgcgg ccgctatttt tggagtggct
     1381 tgcttgaatg gatgcaaaac gtgttggtgg ccgagaaagt catcagcccg cacgacatgg
     1441 atttggtaca aattgtggat gaacccgagg aaatcgtgcg catcatcttc cagcattacg
     1501 aaacctcggg gatgaatcaa aacgagttga cgtatcaaca gcaattgagt ttgtaagcat
     1561 caacatgcat caaatcattt acaggccgcc tacaagcggc ctgtaatgca tggtgcaaaa
     1621 gaatgggtag ctttgaatca agaaaatgtg ggcttattgg gagatagatg ccaaaaacgg
     1681 catgaaaaat atatctgcta gaacaataat ggcgtttatc actgcttaaa tttttagcaa
     1741 actgtttgta agcctgatgc gcatattcgg gcaacaaata caaacataag cttgaataga
     1801 gcaaggaaaa agaagatgaa aacacaagta gcgattattg gtgccggtcc ttcgggtttg
     1861 ttattgggtc aaattttgtc caaaagtggc atagacaatg tcattttgga acgcaaagac
     1921 ggcgattatg tgttgggtcg catccgtgca ggcgtgttgg aaagcgtgac ggtagagggt
     1981 ttgaagcgtg caggcgtgac caaaaaccta gaagccgaag gcatcccaca tgatggttgt
     2041 aaaatggtgt tcaatggcaa ggcgtatcgc attgattttg aagccttggt ggggcgccat
     2101 gtgacggtat atggtcaaac cgaggtcacc cgagatttga tgcaagcgcg tgccgaaatt
     2161 ggtgcaacaa cggtttacga tgccagcaac gtggccttgc acgatgtcga tggcgatgcg
     2221 ccgtatgtga cgtatgaaaa agacggtgaa actcaccgtt tggattgtga ttttattgca
     2281 ggctgtgatg gttttcatgg cgtgtcgcgt caaaccatcc ctgccgatgt gctcaaaacc
     2341 tatgagcgcg tctacccatt tggttggttg ggcatgttgg ccgatgtgcc accttgccac
     2401 gaagaattga tttacggtaa gagcgagcaa ggtttttact tgtgcagcca gcgctcttgc
     2461 cacgaagaat tgatttacgg taagagcgag caaggttttt acttgtgcag ccagcgctcg
     2521 aaaacccgca gccgctatta tctgcaagtg gcgctgactg aaaacgtgga agactggtca
     2581 gacgagcgtt tttggaatga actcaaatcg cgcatgttgc ctgaggatgc caaaaacgtg
     2641 gtcatgggta ccagtttgga aaaatcgatt gcaccgctgc gcagctttgt caccgagccc
     2701 atgcgttaca aaaaactgtt tttagcgggc gatgctgccc acatcgtgcc acctacaggc
     2761 gctaagggct tgaatttggc cgccagcgat gtgatgtatc tggccgatgc gtttgaagac
     2821 tattaccaca atcaaagcga agcgggcata gatggctatt cggctcgttg tctgaagcga
     2881 gtgtggggtg cggaacgctt ctcttggtac atgaccaaga tgttgcacac cttccctgat
     2941 gatgaaatgt gggacatcaa aatgaaagaa gctgagttca accaattgtt gaattctgaa
     3001 tacggtgcca agcttttggc cgaaaattac acgggtttgc catactgagt accgtgctgt
     3061 aagctgcgaa accgccttgt atagaggcgg tttttttatg cgatattggt ttacaggccg
     3121 cttaaaaact ggttgaactt gcactgtaac actcacgaaa ggatgggcat gaacctaccc
     3181 gaagccttgc atttggggcc tttgacttta tcgaccacgg tggtgatgtg gctggtggtg
     3241 ttgggctttg cttcatggct gggcgcacgc atcgccaaaa cccacaatgt ggcatttgat
     3301 ggctggtttt acggcgtgtt gctgtttgcc attgtggtgg ggcgggcggt gtttgtgtgg
     3361 caatactggg cggaatacgc aggtgtgttg ggcaaaatca ttgatgtgcg cgatggcggt
     3421 gtgcatgtgg gcgcggcttt gatggcagcg ggcttggctg cgctggtgtt gttggtttgt
     3481 aaaaagcctt tgcgcaaagc ggtattggcg agcttgttaa cagtatatgt gggtgcggcc
     3541 tgtatccatc aatttggccc caatgcggcc catcaaggtt tgcgtttgcc gaatatggcc
     3601 ttgcaaacgc ttgcgggtga aacgcgctca ttgcaagact tgcaaggcaa gcctgtggtg
     3661 gtgaatctat gggccacttg gtgtccgccg tgtcggcgtg aaatgcctgc tttgatgcag
     3721 tttgccgaga aaaatccgca agtggtcgtg gtattggtaa accaaggtga agaggcggca
     3781 acggtggccg cgtatttgca acaagaaaaa ttatccagtg atgcggtttg gctagacccg
     3841 cgcagtgaca tgggggtgtt tgtggaacag caagccttgc caactacttt gtttttcgat
     3901 gcgctaggcc gtttgcaaga ggtgcgtgtg ggcgaattat cggaagccac tttacagcaa
     3961 aaagtcagca ctttacaggc cgcccagcct tgataggtgc aaacaaaaca ggatgaacat
     4021 gacagaatct ctcgatgcac gtttgctgtg gctggctgag caagtgcggc cgaatgtgtt
     4081 ttttatcgac ataggcagtg accacgcgcg tttgccgatt tatttggtgc aaagcgggcg
     4141 catcacacag gccgtagcca gtgacattgc cacaggtgcg gtggcacaag gccgaacgca
     4201 tgtggcagag caaggtttgg aagcgcaaat agccttgcgt ttgggcaatg gcttgcaagg
     4261 catggatatg cctgcggtgg ctgacattgc catttgtggc atgggcgcgg acagcatttg
     4321 cgccatcatc agcgaccaac cccgtgtgaa acaagggcaa acacgcttgc tgctgcaagc
     4381 gatgacagac acgcctgtgc tgcgccaata tttggcggca gaaggctttg gcatagaagc
     4441 ggaagactgt gtgtatgcgg aggggcggta ttaccaatgc atggtggcgg tgtacacggg
     4501 agccgtgcgt gagttggatg tggcggcagc ggaactgggc gaattgaacc tgcgacagcg
     4561 cagccctgcg tttttgcaat cagtacgcga gcgtgagcag cgcttgcaaa aatggttgcc
     4621 catcaaggcc aatgcaggac aagacactgc gctggatacc agcttattgg cggtgtatgc
     4681 ccaagtttta agggagcatt gagtctcaaa atgactttac aggccgctta cgacaggttt
     4741 tgaagccaag ttgaacaaat ctgacaaata agcatccatc acattcatga tgcgaggcat
     4801 ggtgtttagc acgctattgc acacagcgaa ggagtggttg atgcaaattt cttaccgcac
     4861 agcgctgctc agcagcctac tgggcttggc cttggtggtg gcgatgcaag cgtattctgg
     4921 cctcacttgc tatgagcaga cttgggatgt gcttttaacc aacataggca tttttgtgat
     4981 ggtgccctta atccctgcct tcatcgcctt gttcacccgc aatcccttga gtgctttggg
     5041 cggtttcttg gctttcctgc cttggctcat ctatgcctat tatgtggact gtatgacacc
     5101 gcacacaggt tcaggtggcg catcgttaat ttatgtggtg gtgtttttat acggcgccgc
     5161 cagctgcttg ctcggggtgt tgtttgtggc ggtattgatg tggttgatgc aggcgaaagt
     5221 ggtgaaaggt cggtaggcct agagatgtca ataatataaa caagcggcct gtaagtgttt
     5281 acaggctgct tgttttggag taaatcaaat tggtattgta agttatctag acatcaatag
     5341 ttggaacaag aaaataataa gtttaagaaa aatgatttat attgccaaag aggcatcgga
     5401 tagctcaaat cgttgtttca agattacact taaatattgg atttttaatg aataagcccg
     5461 ttgcattgcc cattctgtag agtatagttg tttacgtagg ctactgctgt tcgcaccttt
     5521 ggtacatgct ccaaggaaac gtgtatcact ttcagataat tgatgagcac ggccttggcg
     5581 aattttatca gtaataatac gccaatcatc ttgaatgact tgtaaatcat ctgcaaactc
     5641 aggactggat aaatcaatga gttgtgcatc aacaatggtg tattcagagc gatggacgcc
     5701 tatttgatat tggtatgcaa acagtaagat agatttgttt ttttgccaaa atgagctttg
     5761 gtagaagtca tctaagtttt cggtttcata gttgatgata ttgcaaacca agcgttcttt
     5821 tgccttaaat ccaccatttc gcacatctgg tttatagcct gtaactttaa tttctaagcc
     5881 tgcttcttct aagtcggctt cagcaaaact gttggcagga atattaaacc aaccttcctg
     5941 taaaatattg cccaatcccc ctttggtatt gcttaaacca tctttcccac ctctgtcaat
     6001 gatgatttga ttgatatcac caaagctttt acccattgac tctttggcac gctgtaaagc
     6061 caattctttg ttggtataga tagtttgcat atattccctt ggattttaaa tatgtaaaag
     6121 ttgatcaatt aagtgacttg tgctaatctt ggcgtttgtt tcaacctatt tgttttgata
     6181 ttgttgattt tatgagttca caaaaaatta ttcgtgtggt ggagttgttt gctggtgttg
     6241 gaggttttcg attagggtta gaacgtacag ataagcaacg ctttcaaacc atttgggcaa
     6301 accaatggga gcccaataaa aaaagccaac atgcatactc atgttacata aaacatttcg
     6361 gctcgtcacc caatcatgtg aatgaagata tagcttttgc taaattctca gtgcccgagc
     6421 atgacttatt agtaggtgga tttccatgcc aagattattc tgtggcacgt acaggtgcgc
     6481 agggaattga agggcataaa ggtgtattgt ggtgggaaat acgggatatt gttcaaactc
     6541 atcgacctga atatattttg cttgaaaatg ttgaccgatt attaaaatct ccagcaacac
     6601 aacgaggacg tgattttgga gtaatgctac gttgtttaaa tgatttggga tatgtggtcg
     6661 aatggcgtgt tattaatgcc gctgattatg ggtttgcaca gcgacgccgt cgcattttta
     6721 tttttgccac tcgtcaagat tctttaatgc ttaagagttt acaagtggat gatgaaagtt
     6781 tgaaagagtg gttgttacac gatggtttgt ttgcacaagc atttcccgtt gagccagaaa
     6841 ttcatacaaa gaaaattaca acatctctta ttgactctaa gcatttttct gaattggctg
     6901 atgtgtctga gcgttttaaa gttgagttgc ataatgcggg agtgatgtgt aatggtaaat
     6961 ttgtttcgtg tgaagtgatg ccaatatcta agcctttcat accattaaaa aatattctac
     7021 acaacgcgtt tgcagtgaag cataaagagc aacagccatt tgaagtagaa aagcgttatt
     7081 ttttaagtga tgatactgaa aaatggcgtt atttaaaggg tgcaaagcgt attcctcgta
     7141 ttaaacccaa tggagaacct tacttttttg ctgaaggggc aatgtgcttt cctgataatt
     7201 tggaagcctc ggctagaacc atgcttacta gcgaatctag tattaataga agttctcatg
     7261 tggtagctga tttactcaat gggcagttga ggcttttaac gccaattgaa tgtgaacgta
     7321 ttaatggttt tgatgataat tggacaaatg tagaaggtgt accacataag tttcgctatt
     7381 ttgcgatggg taatgcattg gttgttcctg tcgttgaagt tttgagtaaa acattattgc
     7441 aaagaattga tgtaaattaa tgcttaaaga ttgattaagc ggcctgtaaa tgtttacagg
     7501 ccgcttgttt tatggatgaa gtttttgaaa atcaattgat taaaattctt cggtatcgat
     7561 ttccaattgg gtggcggcgg ggtagcgggg gcctgaaacg gtgtgttgat taatcaggtc
     7621 gcttgcttgc gccaagatgt tggtggtgat gttgagggtg ggggtggcga tgttgtcggc
     7681 caagtgttgc aagctggtcg tgcctgggat ggcgacgatg tggtcggctt gggcgagcac
     7741 ccaatacaag gcgagttggc cgggggtgca gcctgcttgc ttggctaagg cggcaaaagc
     7801 gtttaacaag gtcaaattgt gttggaaatg ttccccttga aaacgcggca tgcctaagcg
     7861 tatgtctttt tcgggcaagg tggtggtgtc gtgcaagaca ttgcccaaga agccgcgggc
     7921 aacggggctg aaggcgacca gagttgcacc tatgtcttta caggccgcta tcgtgcccaa
     7981 ttcgggattg cgcgtccaca atgagtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatgtt
     8041 cggcatgggc cagtttgagg gtggcagcgg acacttcgga caagcccaaa tggcgcactt
     8101 tgcctgcttc gaccatgcga cccatttcgc ccacgctttc tgcgatgggg atgtttttgt
     8161 cgtagcggtg caggtaatac aaatcgatga catcggtttg caagcgttgc aaagattcat
     8221 cgatttggcg gcgtaaggtt ttggggtggc catcgatgac gcgtttgcca ttcacaccgc
     8281 ccatgccgca tttgctggcg aggaaaaact gttggcgttg gccttttaag gtgtttccaa
     8341 gcagggtttc attgttgcca aagccgtata aggtggcggt gtccaggtgg ttgtagccca
     8401 aatccaaggc tttgaagagc agggtttgtg cgtcttgggt ggatgggggc gtgccatagg
     8461 catggcttaa attcatgcag cccaaaccga tggggttgac gggagtggcg gccaaagtgc
     8521 gtggtgcaaa catgaaacat ccttttgtgt ggggtaaatg gcaaaacacc catcaggcgc
     8581 ttgtacgcag cctcatgggt gtgggttgat acgcaacaaa gcttaggctt gtaattgcaa
     8641 ctctaaggct tgcaaggtgc ggtagcaatc aatcaccgat tgcaccgatg agttgggctc
     8701 gcggccttcg aggatggcgg ccacaaattc acggtcttgc aattcgatgc cgttgtccga
     8761 tacggtcacg cccgtcaaat ccacaggctc ttctttgcca gtgaccaaat cgtcgtaacg
     8821 ggcaatgtaa gtgccattgt cgcaaatgta acggaagaag gtgccaatag ggccatcgtt
     8881 gttgaatgac aaagacaagg tacaaatcgc gccattagcg gcctgtaact gtatcgacat
     8941 gtccaaagca atgcccaatt ctgggtggat tgggccttgt acggcattgg ctttcacgat
     9001 gggcgcgcct gcttggtagc ggaacaaatc cacagtgtgg gcagcatggt gccacaacaa
     9061 atggtcggtc caagagcgtg cttcgccttt ggcattgatg tttttgcggc ggaagaaata
     9121 ggtttgcaca tccatttgtt ggatgttcaa ttcgcctgcg gtgattttat tgtgcaggta
     9181 ttggtgtgaa ggattgaagc ggcgggtatg gcctaccatc gctaccaagc ccgtggcttg
     9241 ttgcaaggcc aacacttctt cggcgtcgcc caaggcatct gccaatggaa tttccacttg
     9301 cacgtgtttg cctgctttca agcaagccaa ggtttgggag gcgtgcattt gggtaggggt
     9361 acacaaaatc actgcgtcta cttggcctga agccaaggct tcgtctaagc tgccaacggc
     9421 atgggcaata ccgtattggt ctgccaccac ttgggctttg tcttggctag agccaacgac
     9481 aaacgtcact tcggcattgt ctatgttttt caagccgtcc aagtgtttga tgccaaaagc
     9541 accggtgccc actaaggcaa tgcgcacttt ttgagtcatg ttgaaatcct tttcttactg
     9601 attttctaaa atcaaatggc caacggcggt attagacgca ggcacgtggt agaagcggtg
     9661 ggtcactttc ggggtttgat cttcgtccat ggcgccgcgt gcaatcaacc acatcaccaa
     9721 ttccacgcct tctgagcccg cttcacgcaa gtattccacg tagtttttct cggccaattc
     9781 ggcaggtgcg ttaatcaaat cgtctaagaa ttgattgtcc cattcaggat tgattaagcc
     9841 agcgcgaggg ccttgcaatt ggtggctcat gccgcctgtg ccccaaatgt gcacattcac
     9901 gtcttcgtcg taagactcaa tcgcgcggcg tatggctttg cccaactcat aacagcggcg
     9961 gcctgatgga atagggtatt gcaccacatt tacgtgcaaa ggaatcactt tgacaggcca
    10021 cttttctacg tcgccaaaca tcaatgtcat cggtacggtc aagccgtggt ccaatttcat
    10081 cttgttgcac aaggtcaaat caaagtcttg ggtgatgaca gagtgggcga tgtgggcagc
    10141 caagtcgggt gcaccttcga tatcgggcac agggcgaggg ccccaacctt cgtcgcaggt
    10201 ttcaaaatgc gcggccgtac ccaaggcaaa agtcggaatc aattccaaac tgaagttgtt
    10261 ggcatggtcg ttgtacacca aaaacaccac gtcgggcttg ttgtctttgt gccattgctt
    10321 ggaaaactca tagccatcaa acactttttt ccaatagggt tcgtcttttt tgcccaagtc
    10381 ccaagcggca ccaatggcgg gcacgtgtga ggtgtaaacg ctggcggtga ttttggccat
    10441 tatttgtctt ccttttgttt gtcttcccat tcgtggaggt agcgattgcc atcaatttgg
    10501 cgaccaccag ccagcatcat gtcgcgataa gcttcttcgc tcatgcccgt catcgagcct
    10561 gccatttgtt ggaaagaatg gccatcggtt gcaccgattt tggccaaaaa gtaaatattg
    10621 ccacccaagt ccatgcagcg attcaaatcg cgatccaata ccgcttgttt ttgctcttcg
    10681 ctcatggccc attcgtccaa atacgcacgc tcgtccgctt tgaagcgggc gcggttttct
    10741 gccgacatca acgacatgca aaattggttt aaatggtagc ctttgcgcga ctgatcggaa
    10801 tcaaacacaa tggtgccagg aatgtcttta tacggttttt caattgccat ggtatctctc
    10861 ttctattgga aacaaacggc ttaagcgact tcttctggcc agtacaagcg catcggattg
    10921 tttaccaaca attgttggcg ctcggcttcg gtcggggcga tttgggcaat gtattccacc
    10981 aacaaaccat cgtcaggcat gtggtctttc aaatttggat gcggccaatc ggtgccccac
    11041 aacacacgct ctgggaacaa ttccatcaag cggcgcgcaa acggtgccac gtcggtgtaa
    11101 ggacgggttt cgccatccaa ggctttgggg ccagtcatgc tcaagcgttc tgggcaagac
    11161 agttttgacc agatgttggg atgctcggcc atcaatttga caaacagatt gaattcttcg
    11221 ccatccacag gcttgctcac atcaggtctg cccatgtggt ctaccaccac ggtggtgggc
    11281 aaattggtga aaaaatccca caattcaggc aaatctgggg cttcaaaata aatcacaatg
    11341 tgccaaccaa acggggcaat tttagcagca atcgcctcca aatcagcctt gggcatgaaa
    11401 tccaccaagc gtttgacaaa attaaagcgc acgccgcgca cgccagcgtc gtgcaaggtt
    11461 tggatttctt cggtggtgat gtccacaccc acggtggcca cgccacgggc tttgccatca
    11521 gaggcaagca aggcatcgac caaggcgcgg ttgtctttgc cgtgacaagt ggcctgtaca
    11581 atcacattgc gtgcaaaacc caaatggtcg cgcaaagcaa acaattgtgc tttggacgca
    11641 tcgcaagggg tgtatttgcg ctcaggcgca tacggaaatt cgttaccagg gccaaacaca
    11701 tggcaatggg catctaccgc acctgcgggt aattttaaag tgggtttgct tgggccttga
    11761 taccagtcca accaacctgg ggttttttca aaaacagttg tgctcatggt gttttcctgt
    11821 cacttagtct atgtatttca ggccggcttt ttccaaaggc tcgcgcattt tgtacatgtc
    11881 gaggcccaat tcacctgctt caaagcgggc acgtttttcg ccctcgaaag attcgcgttt
    11941 ttcggcggca tcggccactt cttgcgcgcg ttcacgtggc actactacca cgccatcaac
    12001 atcggcaatc accacatcac ctgggttgac caattggcca gcacaaatca caggaatgtt
    12061 cactgagccc aacgtggctt tgatggtgcc cttggcatgt atggctttgg aaaacacagg
    12121 gaagcccatt ttttccaatt cgtccacatc gcgcacgccg ccatcaatga tgaggccttt
    12181 ggcaccgcgg gctttgaatg aagtagcgag caaatcgcca aaaaaaccat ctgcattgtc
    12241 ggtggtacag gccgctatcg caacgtcacc tgcttgcaat tgctcggcgg ccacgtgcat
    12301 catccagttg tcgcccggtt gcaagagtat ggtcacggcg gtgccgcaaa ttttggcgcc
    12361 gctgtataca gggcgcatgt atggcttcat gagaccgaca cggcccatgg cttcgtggat
    12421 ggtggccacg ccaaattgcg ataatttggc cactgcggcg gcatcggcgc gttcgatatt
    12481 gcgtttgacc acgcccaatt ggttcaatgt cacttccatg attacaatcc tttggctttc
    12541 aattgtgcgt ctaagcgtgg gaacacacgg cgcgtattgg cttcataaat attgtgtttg
    12601 tcttgcgcag agattttcgc cgcatcaatg tatttcttgg tgtcgtcgaa ataaaaacct
    12661 gtgcgtgggt cgatgtcgcg cactgcacca atcatttctg aggcaaacaa gatgtttttg
    12721 ttgggcacca catccagcaa taaatcaatg cctggctggt ggtaaacgca ggtatcgaag
    12781 aagatgttgt tcatcaaatg ctcttcgatt tcgggttttt tcaaaatcat cgccaaaccg
    12841 cggaaacgac cccagtggta aggcactgcg ccaccaccgt gtggaatcaa gaagcgcaag
    12901 cttgggaaat ctttgaacaa gtcacccgtt aagcattgca tgaatgcggt ggtgtctgcc
    12961 gccaagtaat gtgcgcccgt ggtatggaag gcagggttgc aactggtgct cacgtgtacc
    13021 atggcgggaa tgtcgtactc gaccattttt tcgtaaatcg gataccagta tttgtcggtt
    13081 agtggaggtg atgtccaatg gccacctgat gggtctggat tcaaattgat gccgacgttg
    13141 ccgtattcgt tcacacagcg ttctaattcg gccacacagg tttcaacagg cactcctgga
    13201 gactgtggca acatggccgc aggcacgaaa cggtcgggat acaattgcgc cacacggtag
    13261 cacaattcat tgcaaatcgc cgcccaagtg gcagacactt ggtagtcgcc aatgtggtgg
    13321 gccatgaaac tggcgcgtgg tgagaagatg gtcaaatcag agccacgttc attcatgaat
    13381 tttaactggt tggcttccag tgtttcgcgg atttcgtcgt cgctgatttt caaatcagcc
    13441 actttcggag cttggcttgg gttgtttaaa gtcgcgattt gtttttcgcg ccaatcggcc
    13501 aaagccttgg gagcggtcgt gtagtgaccg tgacaatcaa tgattaaact catgttctct
    13561 tcaccttttt aaagagtgac agacaatagg ctgcgggcct tgctgtcaaa atcaaattct
    13621 gtgatgtgag ttggtttgga tttgcgactt taaatcatta caggccgctg ttgttgtgtc
    13681 atcccatcca ttgccaccca ctgtcatttg atattgccgc aagcgagcca tttaaaaaag
    13741 atgcacttgt ttctattagt tatgcgtgat tgacatagat aacgatacaa aaccatcaaa
    13801 aagcggcctg taaggcttac aggccgcttc aacatgttta aaaacacaga acaccaaaga
    13861 aatcaattgg ttttaagcct gtgcacccaa gtgtgatgcc accatcactt cgccgcgcat
    13921 gatgaggcgg gcggtgcgca acaaggctgc gctcaatacg ttggtggcat cgtcggcagc
    13981 cgtggtcaat tccacgctga attcgccgct aggatgctct atcgacatca aactgctatt
    14041 gccttctttt ttgcttgcga cggccaagcc atcggccaca ctgccaggca atacacaggc
    14101 tgtggccacg gtcaccgccg ccaacactcc gatggcatcg tgacacacat gcgggatgta
    14161 gcaacgcgtg tggatggcgc cgccattgat ggggctgttt agcaaacaca tttttggata
    14221 gtttttgttc gatacatcgc ccaaacccat tttcaatgcg gcctgtaaac gcaattgttc
    14281 tattttggct ttcaaatcgg catcttgatt caaatcggcc acgctttcgt aagccgttcg
    14341 atgcatgtcg ttggcgcgca ccaacaccat gggcatgccg ttgtcgatgc aagtggcctg
    14401 taaatcaccc acgccatcaa tgtgaaacac atcgaccaca ttgcctgtgg gcagcaatga
    14461 gcctgccacc gagcctgcgg tgtctaaaaa attgatttta atcggcgcgc tgtgaccagg
    14521 tgcgccatca atgcgcgcat cgccttcgta gtcgatttcg ccattgggtg tttgcaccgt
    14581 gatttcagcc accatgcctg tgttaagcgt cagcacttta acggtggtgg tgtcgccttg
    14641 ggcggtaatc aagccttttt ccagcgcaaa cggcaccaca gctgcgagca tgttgccgca
    14701 attgggggtg gtgtctactg tgtctttttc aggctgcaat tgggcaaaca aaaagctcaa
    14761 gtcggcttca tcgccagcac acacatcgac gatgccgact ttgctggtaa gcgaatgcgc
    14821 accgcccaag ccatcgattt ggcgcttgtc tggcgaaccc atggcagcaa gcaataccgc
    14881 atcgcgcgcg gcgatgtcag tcggtaagtc cttggcgtaa aagaaaggtc ctttggaagt
    14941 gccgccacgc atgagcatgc atggaattgc cgtttgcttt gccattttcg tctcgctgtc
    15001 agtgatttat catctatact ggtgcgcagg agttgcgctt ttagagtgca tttccccgca
    15061 ttgtcggtgg tattatgaaa cggcaatcag tgtgaaaaca gcatggcaac agtctatgtg
    15121 ttatgtattt ttgttataga aagcggtgaa tacagtggaa ttgaaacaac tcaagtattt
    15181 catgcatgtg gcggaattgg gcagctacac gcgtgcggca gaggtgatag acgtggcgca
    15241 acccgtgctc agtcgccaaa tccgccagct ggaaatcgag ttgcgtcaaa acctgctcat
    15301 ccgccacggt cgtggcgtgg tgctgaccga ttcaggccgc accttgctca aacacgtcaa
    15361 catcatcatg gaccaaatcg acgaagccta cgaagacttg agcctgtctg atggcaagct
    15421 cgcaggccac atcaccattg gtttgccgcc caccgtggcc aagctcatcg ccattgattt
    15481 ggtgcgcgct ttcaagcagc gcctgccttt ggcccacctc accatcgaag agggattaac
    15541 cgttcacatt gaagaaaact tgggtttggg gcgcatggac atcggcttgc tcaacaaccc
    15601 cactttttca cccaacatcg acaccaaact catcatcgaa gaagaactct atctcatcgc
    15661 cagaaaaggc tatttccccc accagcctga gtgcatggat ttgaaagaat tggccacgat
    15721 acctttaatc atgccgactt tgcctaatcc atttcgggtg ttggtgcagg cacaaatggc
    15781 ggaaattggc ctcaagccgc agattgattg ggaaattaac agcgtgggca ccatcgtgaa
    15841 attggtcaac gagggcgtgg gctgtgcggt gttgtccaaa tactgcttgg gcctcgtcag
    15901 agacggcgac aatatggaag ccatccgcat tcaaaaccca gccttaatca accgtttata
    15961 cgtcggttat tctgccaaac gcgccaccac ccgcttgcaa aaagaaatgc tgcaattggt
    16021 ggaagaggtg tgtcagcagt atttttgaag cattgctgag tttacaggcc gcttgtatag
    16081 agtaaacaag cggcctgtaa acttgttgct tacccactga tgtccaaatc ccatcctttt
    16141 gtccgatgcg agcaatgccg ttgcaatcat cggacaagtc ttgcctgcct gcgctgacgc
    16201 aagggcggtt tcaattttat attccaaaca ctttgcccat gcgctgcgct gatgcgcggg
    16261 ctggcgcaag gtggatacca ccacaataag ttgggcgtgt gcttgtgcat gccgccacac
    16321 agtgactaca aggaagaaaa cgttgaaacg ccacctgatg acaggcttgc ctttggctgc
    16381 cttatgcgtc ttgccgactt tggcttttgc cgatgatgca agcaccgaaa acaccgccaa
    16441 cgccaccaac gctgacagcg tgtgggcatc gccttttttt gatgatgcca agttgtcggt
    16501 attgaaccgc aattattatt tcaaccgcga ttttcgccac ggcggtgcca atccgtctgg
    16561 cagcaatcaa ttcaaacctg tgggcgagcg caatggctac cgcagtgagt gggcgcacgg
    16621 cattgttgtg gatgtgcaat cgggctatac cgaaggtgtg gtgggcttcg gggtggatgc
    16681 acaagccttg gtgggcgtgc gcttggacag ctcaggcgca cgcacgggca cgggcttggt
    16741 acctgtgggt aaggacggag aacctgccca cacttggggc gaagtcagtg gagccgttaa
    16801 agccaaagtg ggcaaaaccg tattgaaata cggcacgcaa atgccgaatg tgccagtgct
    16861 gtcggtacac aatgcgcgtt tgctgcctgc caccgtgaat ggtgtgtcgt tggagagcaa
    16921 tgacctagac aaattgaatt tacaggccgc ttggtttacc cacaccaatg ccatagacag
    16981 caccaaccac gatgaccgat tcaataccga ttatggcttt tttttggcgc ttgtggccaa
    17041 gcgcgcaggc tatgtgggcg gtacttacaa atggtcgccc gacacacaag cgagtgtgta
    17101 cgtggggcat ttaaaagacg tgtggaacca atattatgcc caaggcaacc acacttggcg
    17161 ctttgataac aatacctttg tgaagctgaa cggcgtgggc tattggaacc gtgacacggg
    17221 caaatctttg gcaggcagca tagacagcaa attgctccga accaacgtga cttacggcaa
    17281 tgccacccat gcgattacag caggttacca acaggtgttt ggcgatgagc ctttggatta
    17341 catcggtttt cgcaccatcg gcggcaatgt gaattttgcc aacatcggca atgtgtcgcc
    17401 gtttgccgag gccaatgaaa aatcatggca attgcgctac gacttggatt tcaaagaatt
    17461 gggtgtgcca ggcctgagtt ttacaggccg ctacatcaaa ggctttgaca tggacaacaa
    17521 aaacagcagc aatcaagtct acaaaaatgc cttaaaaggc tatacctacc gcgatggcga
    17581 cagccattgg gagcgcgatt ttgaattgaa atacgtgttc caaagcggcg ctgccaaagg
    17641 cttgtctttg cgtttgcgtc aagccacttt gcgcgcttcc acaggctacc gctacaacaa
    17701 catggatgaa gtgcgcatca ttgccgaata cccatggtct ttataaggaa gcatcatgca
    17761 aaccgtttat gtgttgaatg gccccaatct gaatttgctt ggcgtgcgcg agcctgaaat
    17821 ttatggccgc gataccttgg ccgacatcga agccttgtgt cagcaaatcg ccgagcaatt
    17881 cggttggcaa ttgcgctttt tacaaagcaa ttacgaaggc gctttggtag agtgggtaca
    17941 cgaagcacgc gccgcaggag cggccgtggt gatgaatcct gcgggctgta gcttttattc
    18001 cattcctttg ctcgatgctt tgtccacact ggaaacgccg tatatcgagg tgcatttgag
    18061 caatattcat gcccgcgatg ccgatcacca gcactccatt ctgtcgaaaa aagcccgagg
    18121 ggtgatttgt ggctttggtg cgcatggtta tgaattggcg ctgcaagcct taaagcaact
    18181 gctgtaagca ttccactcag gggtattaag tcaataaaaa atccgccttg tgggcggatt
    18241 ttttgcgttt gagacgggag aaaagggggt gaaggcgtga tgtttgtatg gattaagctc
    18301 caaatggttt tacaggccgc ttataacagc atgcgcaatt gtttggccaa ttcttgcatg
    18361 ggcaccaagc agcggttcgt cacttgttca accgtgtatt gctcgctgcg cgccaccgta
    18421 ttcaaacccg ccaccacccg acctgagcca tcaaatatcg gcacagaaaa gccgatgaaa
    18481 ctgacttcgt gttcttctac tgccaaacac caaccttggt cacggatgtc gtgtaattgt
    18541 tgtttgaacg tatcgctgtc ggtaacggtg tatttggtgc tgcgggtgag tgggtaatgc
    18601 tctagccaag tgtccaattc ttggtcttgc atataggcca aaatgatttt gccattagaa
    18661 gcagtgtggg cggcgatgcg gttgccattg tgaataccgt atggcaagac gcggtttttt
    18721 tcggcactgg ccaaataact gcaggccacg gtaatcactt cgtggttgtc caaaatcacc
    18781 accgaatggc acatgcctgt ttgagaagac agtgaatgca gcaaaggctg ggcgattttg
    18841 ggcaaatgcg ctgagcttaa atacgcgcct gaaaatttca acacgcggtg ggtcagccaa
    18901 taaaacgtgc catcactgtc taaatagccc aagtatttta aggttttcaa atggcggcgt
    18961 gcggcagtgc gggtgaggcc agtgcgttcg gctgtttggg tgacgttcag gcgctgcctg
    19021 tcgtagccaa aacactccaa cacttgcaaa cctttggcca agccagcaat gaagtcatcg
    19081 tagcgaatga tttcttgcgt gtgctcatgc acaatttcgg tgtttctcat ctcttttctc
    19141 cagagtgaaa cggcttcttt attgttttta tggctcttgt gcgatatcag caagcttggt
    19201 gcgattatcg gacagaccat gatacacgct gttggtttgt cgcgccgatg tgcttagtat
    19261 catgggcagc ggtgtgcagc ttagacccat gttgtgtgcg cagcttagag ggtttacagg
    19321 ccgcataaaa aggaacaaac ggttataact gttatcgaca aaacaaataa caccccatct
    19381 ataaggaagc gcaacatgcg attgaatcaa aaaacagcca tcgtcacagg agcaggctca
    19441 ggcatgggtc aagccgaggc cataggtttt gccaaagagg gcgcgaaagt ggtggtggcg
    19501 gatgtgaatt tggccactgc tgaggccacg cgcgatgcca tcgtggcagc aggaggcgag
    19561 gcgattgccg tggccgtgga tgtgacctac ttggcggatt tgcaaaatct ggtagatacc
    19621 acactggcaa cttatggccg catcgatgta ttggtaaaca atgcgggtat ttttgattac
    19681 tacaccaaca gcttggacac cagcgaagac ttgtgggaca aaatttttgc catcaatgtg
    19741 aaatcgatgt ttcagctttc caatttggtg ttgccacaca tgcaagcaca aaaacaaggc
    19801 gccatcatca atatttgctc catcgcaggc ttggtggcga acatgggtgg cgcggcttat
    19861 accgcatcaa aacacgcggt ggctggctat accaaacact tggcggcggt gtatggcaaa
    19921 gacggcatca aaatcaatgc catttgccca ggcaccatcc gtaccgcgat gacagcagaa
    19981 atgcttaaaa cccgacctac cgacaaaatt ccattagacc gctttggcga agtgggtgaa
    20041 gtggtggatt tggctgtgtt tttggcttct gacgaagcac gttttatgaa tggcgctttg
    20101 gtgccgattg atggtggctt taccattgta tgagtctctt ctgtcgtgaa tgacaggtct
    20161 tgatgaccac tgtgttccca cacagtggtc tttttttgtt tgtggacgaa ggtcggacac
    20221 tgcgtccgat agtcggacaa atcaactgtg tcgcttctag gcagcaagat gtcgcctcct
    20281 tatgcttcat tcaccctaaa aaagggctga agagaagcac taaaaaaaca aacaaaatca
    20341 acaaatatca tttaggagaa gtgtatggca actcagttga atgtgcggga gttcatagat
    20401 gaacagccgc tgtcgggatt tcaaaaatga atcattgcgc tgtgtctgtt ggtggtcatc
    20461 gtcgatggct tggacgtggc ggtaatgggt tttatcgcgc cttcattgaa agaacaatgg
    20521 ggcattagca attcggattt ggcacctgtt atcagcgccg ctttgtttgg tttgaccata
    20581 ggtgcgatgg tggcagggcc gctggcagac aagtttgggc gtaagaaagt cttgctcgtg
    20641 tgtgtgtggg gctttggctt gtttaccttg gcaggtgcga tggccgattc ggtgtcgcat
    20701 ttggtggtgt tgcgttttat tgctggcctg ttcatgggcg gggtgatgcc gcaatgcgtg
    20761 actttggcga cagaatactc acccaaacgc atcacttctt tcttaatcac cgtggtgatt
    20821 tgtggctttt ctttgggcgc agccatgggc ggctttttgg cttcgtggct gattcctgca
    20881 tttggttggc aatcgatgtt ggtggtggca ggggttgtgc cttgtctgct ggccttggtg
    20941 ttgacgtgga aactgcctga gtccactggc ttcatgattt tgaaaaaaca gcccactgca
    21001 aaaattcgcc gctacatcga aaaaatcgcc acccaacctt tgccacaaga cgtgcaattt
    21061 gtcttgcctg ctgcgctagc caaagccgat aaaccgatgc aaaccgtgct caacagccat
    21121 tatttgattg gtactttgtg cttgtggggc tgttatttct ttggattatt tgcggtgtat
    21181 ttgttgggca gttggttgcc attgattacc aaagaagcgg gcttcagcac ttctgaagca
    21241 tccatcatcg cggcggtatt tcaattgggt gggcctttgg gctgcatcac cgtgggttat
    21301 ttgatggaca agttcggcaa tcaccgtgtg ttggcgctgt gttttgtggc agtagcggct
    21361 tcgttggcgg ccattgtcat ggccagtggc agttttgcct tgttctgtgt ggtgtgtttt
    21421 ttaatcggtg cgaccttgaa tggcggcaac acgggcttga atgctttgtc cagtgtgttc
    21481 tatcccgtgt ctgcgcgtgc cactggcaac agttggatgc acggagtggg ccgtgttggt
    21541 gcgattttga gtgcgtttgc gggtgcccat ttgctcaatt tgggctggca catcgaccgc
    21601 attttcttga tgttgatgat tcctgctttg ttggtgatgg cgtgcatcgt catcaaaggc
    21661 atgcgctatg gcaaaaccca gcctgaagtg caattacaaa acaaaaccgt gtgaattcag
    21721 gaggagagag aaatgaccat ccaattaaat ggtgccagcc gtgtttatta cgtggtgggt
    21781 gatcccatcg cccaagtgaa atcgccacaa ggcgtgaccg aattgatgca acaacaaggt
    21841 gtgaatgccg cctgtattcc tgcccatgtg gcgcctgagc agttagcagc gtttgctgtg
    21901 ggtgtgaagc ccattcaaaa catcgacggc gtgattgtga ctgtgccgca caaaatggct
    21961 tacaaaaatt tgtgtgacag cgtgtcgcca gaagcggcat ttttaggctc ggtcaacacc
    22021 gtgcgcaaaa cagcgacggg ttggcatggc gacatgtttg atggccaagg ctttgtcgcg
    22081 gctttgctga aaaaaggcgg cgtgttgcaa gacaagaaag tgttgttggc gggcgcgggt
    22141 ggtgcgggtg gcgcgattgc ctattccttg gtgcaggcgg gcgtggcgga attggcaata
    22201 gccgaacgcg atgcggccag acgcgatgat ttgattgccc gcttgcaagg tttgggattg
    22261 ggcaaaatca gcgttggctc agacgaccca agtggctttg atgtggtgtt gaatgccaca
    22321 ccttgtggca tgaaagccga cgacccgttg ccgtttcaag ttgataagtt acaggccgct
    22381 gtgtttgttg gcgatgtgat taccgcacca gaggtcacgc cattcatcgc cgcaggccgt
    22441 gccttgggct gtccgtccat gttgggcatg gacatgtttg ccgaagtgcg cgacttgatg
    22501 gtgcagtttt tgttgcaagg cgaggaggtg caagcatgag ccgctcttac tctttgtcgt
    22561 ttttgacggt acaagaagcc agccctgttg aagcggtgga aatcgcggca gcctgtggct
    22621 atgactatgt cggtttgcgc attttgcccg ccgccgcgag tggcgaagct gcttaccctt
    22681 tgcttcacga tgcgaacctg ttgcgtgaag tacaggccgc attgcgcgac acaggtgtga
    22741 aggtggcaga tgtcgaaatc gtgcgcttga aagacagcta ccgtcgcgct gattttcagc
    22801 cttttgtgga acgtgctgcc gctttgggcg cggcgcatgt gctggtggca ggtgacgata
    22861 ccgatgctgc gcgtttgcaa gactcttata ccgatttttg ccatttggcg gctgaatatg
    22921 gcttgagcgc cgatttggaa ttcatgccat ggacggccgt gaaaaacctg cgccaagccc
    22981 gagccatggt ggagcaaagc ggcgctgcca atgcgggggt gttaatcgat tcgctgcact
    23041 ttgcccgttc cgattcgagt ttggcagaag tggcggattt gcccagcgat ttgattcatt
    23101 acattcaatt gtgcgatgcc tatgcccaat acgacgacag cgatgaaggt ttgattgcgg
    23161 tggcgcgtgg cgcacgtttg ttgcctggca gcggtgaatt ggatttgctc agcttggcgc
    23221 aattagtgga cgccagcaaa ccgataggct tggaaattcc caatgccgaa ttggtacagc
    23281 atttcagccc caaacagcgt gcacaaatgg ccatagacca tgcccaaaaa ctgttggcaa
    23341 cagccatggt agggggtgag tgatggaagc catgcacaca gcccgtgaca ccgatgtgtt
    23401 gattgtgggc tcaggtgcca gcggtttggc ggcggccatc gccgctgccc acgcaggcct
    23461 gaaggtggtg gtggccgaaa aagccaatgt ggtgggtggc accagtgcat tttcaggcgg
    23521 ttggctctgg ataccgcatg caccgcacac caaggggcaa aacacgggtg acaccgcagc
    23581 cgatgtcgag gcttatttac aggccgtgtt gggcgagcgc tacgacgaca ccatgatgca
    23641 gcagtatttg caacgtgcgc ctgaaatgtt ggcatttttt gagaaccata cggcagtgaa
    23701 atttttgcca ggcagcgctg tgcccgattt ttttggtgaa ttggcgggtg cgaaagcagg
    23761 ttggcgttcg gtggtggccg caccttacga tggccgtggc ttgggcaaaa acctcgccat
    23821 gctgcgccgc ccgatacctg aaaccacgct gtggggcatg ggcatcgcct ctggtgcgga
    23881 catgaagcat ttcttgcaca gcttcaaatc gtggtcttcg ctttattacg tgggcaagcg
    23941 cgtgagcaaa cacggtcgcg atgtcttgtt gcatggtcgc agccaacaga ttgtgaacgg
    24001 caatgctttg gtggcgcgct tattggaatc ggcgctgcgt ttgaacgtgt cttttgtact
    24061 caatgcgcgt gtgtcagaca taggtgaggc agacgaagcg ggcgcgcgca gcgtggtagt
    24121 ggacacgccg caagggcaaa cgcacattca cgcaaggcgt gcggtggtgt tggcggcagg
    24181 tggttttcca cacgatgtgg tacgccaaag ccaattgttt ccacacgtga aaaacggcac
    24241 gccccaccat tctgccgccc ctaccaccaa cacaggcgat ggcttgaatc tgggcgagcg
    24301 cttgggtggc gaagtggaca gcagccaagc ggcggcaggg gcatggagcc ctgtgtcttt
    24361 ggtgccgcaa gccgatggca catggggacg ctttccacac ttggttgagc gtggcaagcc
    24421 aggattgatt gctgtgcgtg ccaatggctt gcgctttacc aatgaaggcg gcccgtatca
    24481 cgcgtttgtc aaagagatgt tggcagccac accagagggt gagcgggcgg tgtcgtggct
    24541 gatttgtgac cacaccttta ttcgccgcta tggtttgggt gcggtcaagc cgtttcccct
    24601 gtcactcaag ccgtggctgg ccaatggtta tttgaaacgc gggaatacct tggcagaact
    24661 cgctcacgct tgtggcatag acgtgaatgg ttttgaagcg acgctgcgcg agtacaaccg
    24721 ccatgccgag caaggccaag atcctttgtt tggtcgcggc agcaatctgt accaacgcgt
    24781 ggcaggcgat gccagcgtgg gccccaatcc ctgtgtggca cccattgtta ctgcgccgtt
    24841 ttacgcggtg gaaatcgtgc caggcagctt gggcacgttt gcgggcctga aaaccaatgt
    24901 ccacgcgcaa gtgttggaca gcacaggtgc ggtcatcagc ggcctgtacg ccgtgggcaa
    24961 cgacatgaac agtgtgatgg gtgggcatta ccccagtggc ggcattacct tggggccagg
    25021 catgactttt ggctatgtgg ctgcccgtca cattgcgggc aagcaggatt aaggaagcat
    25081 tatggaacaa aaaacagtcg atttattggt gattggtggc ggtgccgcag gcatgactgc
    25141 cgcgctgaca ggccgcatgg aaggcttaga cgtattgctg tgtgaaaaag ccaagcaagt
    25201 gggcggcacc accgcaactt cgggcggcac cacttgggtg cctggcaccc atttgagcgt
    25261 gcaaacaggc gtgcccgata aggttgaaga cgcacgggcg tttttgaaat cggtggtggg
    25321 cgagcgcggt ggcgagcatt tgcgtgaggc atttttgcgc tcaggcccag acatggtggc
    25381 nnnnnnnnnn aaatttgccg ctgctttggc ccaccccgat tacgtgaaga accaacctgg
    25441 tgaggcgttt ggtggcaggg ctttggcacc tgtggcgttt gatgccaagg tgttgggcga
    25501 cgattttgac cgtgtgctgc caccgcgccc tgaattcatg ggtttgggcg gcatgatggt
    25561 gaaccgcaat gagttgtcgg ccttattgcg gcctgtaagc tcggtaggca atgtcaaaac
    25621 cacgcttaaa gtggtgttgc cttatttcaa agaccgcttg aaatacaagc gtggcacccg
    25681 tttggttatg ggcaatgcct tggtgggacg tttgttgtac agcttgaagc aataccaaac
    25741 cgaaatatgg tatgaagcac cgttgcaaga gttgttactg gaagaagggc gcgtcatcgg
    25801 tgcgattgta gacacggtca acggtcgcca gcgcatcatc gcgcgcaaag gcgtggtgtt
    25861 ggccacgggt ggcgtggcat ggaatcccgc tttgcgacag caatacttcc ccgtgggcac
    25921 gcgtgactat tccctcgcgc cgcaattgag tacgggtgat ggcttgagca atggcctgaa
    25981 agtgggggcg aaattggata acgaagacca tccagtgctg tggtttcctt gttctacttt
    26041 caaaaagccc aacggtcaaa tggcggtgtg gccacacatc attctcgacc gtgccaagcc
    26101 aggcttgatt gccgtcaatg ccagcggtaa gcgctttgtc aacgaaaccg attcttacca
    26161 cgatttttgc gtggggcagc ttacctgatt tgcgacgacg cattcatcca caaatacggc
    26221 ttgggcctca tcatgccagg cgcacaaaaa ctcaagcatt accttaagca tggctatgtg
    26281 gtacaggccg ctacggtgcg cgaattggcg ggaaaaattg gcgtggacgc acatgaattg
    26341 gagcaaagcg ttgtccgcaa caatgcttac gccatcacag gcgaagacct agagtttggt
    26401 cgaggcacag gcaagatgaa ccgcttcaat ggcgatgctg ccatcggccc caatccctgc
    26461 atcggcccga ttctcaccgc acctttttat gccttggcag tgaagccgat tgattgcgcc
    26521 agcagtgttg gttttgcagg cgatgaatat gggcgtgtct tgaaccaaca aggcgttgcc
    26581 atccatggct tgtatgcctg tggcaacgat ttggcctcca tcttcaaagg cacttacccc
    26641 ggccctggca ccaccatagg cccaggcatg gtctttggct ggcgcatcgc caaacatgca
    26701 gctggcaaat tggctcatga aagcggcctg taaaccccta ttaacaccgt attgacattg
    26761 atttgaaacc aaaaaaggac atggtatgta ttattatgaa ttggccactt tagacatcca
    26821 aatgggcaaa gccgcagacg tggcagcagg cgtggaaaaa tttgtggcag aagcccttac
    26881 aggccgctta ttgggcgtgt ggttcagcga cattggcgcg ttgaatcaag tattggtgtt
    26941 gcgtgaattc aaaacattag acgaattaaa ccaagagcgt gcgcgctttg cccaagcggc
    27001 aaatccgttt tatgcacaag aatggttgga gcacgtgagc gtggattctt accaaggctt
    27061 cccattcttg cctgaagtgg aaacaggcgc gtttggccct gcttacgaaa ttcgcactta
    27121 ccacatgaaa catggcggct tgccgcacgt gcatgcggca tgggaagcgg ccttgcctga
    27181 gcgcaccaaa ctgtcgaaat tgactgtggt gatgagcagc ttggacggcg agccacgcat
    27241 cgtcaacatt tggccatacg actcggtgaa tcagcgcagc caagtacgcg ccgatgcggt
    27301 ggcacaaggc atttggccac ctaaaggcgg cccgcaatgg ttgaccaatg acatggcttc
    27361 tttgattgct ttgccaacca aaaactcgcc tttgcagtag tttattgcgt cgcattaaaa
    27421 aagaccgtgt cgtgcacggt cttttttttg tcgtatttgc gcgattagcg tgatgcgatt
    27481 gcgaatatcg cacacatgga cggtttaccc cattctcagc ctgcacgata aatcctactc
    27541 tagccgcact tcccgcaata agcaaaacaa attcagcaag gagaggcgaa tgtcattgca
    27601 aaacaaaact ttaaatgtgg atgccgtgtg tgacttggtg gtggtgggtt ctggcgctgg
    27661 tggcttgtcc accgccatca cggcgaccaa acgcggcctg aacgtcatcg tggtggaaaa
    27721 agaagtcgtg tatggtggca ccaccgcatt ttcaggcggt gtgctgtggg tgcccaacaa
    27781 tccccatgca caaaagcaag gcatcgccga cagccgtgaa gcagcgatgc gctatttaca
    27841 aaacgaagcc ggcgagcatt ttgatgccga agccgcagcc gtgttttttg acaatgcccc
    27901 gagaatggtc gaatttttcg agcgcgaaac cacggtgaaa tttgtgccaa cgctgtaccc
    27961 cgattaccac cctgatgtgg atggcggtgt ggacatcggc cgctcaatct tggccgcgcc
    28021 gtttgacatc cgtgaattgg gcgatgaaat ggcgcgtctg cgtccgccat taaaaaccat
    28081 tactttcatt ggtatgatgt tcaattcatc caatgccgat ttgaaacact ttttcaatgt
    28141 caccaagtcg cctacctcat ttgtgtatgt ggccaagcgc ttggcaaccc atgtgaaaga
    28201 attgacgctg tacaagcgcg gcattcaagt cacctcaggc aatgccttgg ccgcacgttt
    28261 ggccaaatcc gcgtttgatt tgaatattcc aatttttacc gaaacggcag ccaaagaatt
    28321 gctgacagaa aacggccgtg tggtaggctt acaagtcaaa accgccgatg gcagcgtgcg
    28381 caagattcaa gccaaacacg gtgtggtatt ggccagtggc ggcttctctc acgaccgtgc
    28441 gcgcttgaaa aagctttatc cgcatgtggc cagcggtggc gaacattttt cacctgtgcc
    28501 cacaggcaac acaggtgatg gcgcgcgttt ggctgaaagc gtgggcgcga cggtcagcag
    28561 cgattacgcc gacaattcgg catggatgcc ggtgtcgcgt gtgccgatga agggcggtga
    28621 atttggggtg ttcccacatt tgctcgaccg ctacaagcct ggtgtgattg gtgtattgaa
    28681 aaatggccaa cgcttttgca acgaatccaa ttcttaccat gacgtgggcg cggcattgtg
    28741 gcgtgcttgc gcgggtgaag cagaaaccgc gatgtggctg gtgtgtgatg ccaaaacctt
    28801 ggcgaaatat ggtttgggct atgtgaagcc cgccccattg ccgcatggca agctcataca
    28861 acaaggctat ttgttcaaag gggcgagctt agacgagttg gcccaaaaaa caggcataga
    28921 tgcgcaaggt ctgcgcgaca ccattgccga ttacaacgtg catgccgcac acggtgaaga
    28981 ccccgcatac ggccgtggca caagcacgtt taaccgctat ttggctgacc ctgagcatca
    29041 gcccaatcct tgcgtggcac ctgtggcaca agggccgttt tatgcggtga aggtgtatat
    29101 gggtgatttg ggtacgttca acggcattga aaccacgccc gtgggcgagg tattggacgc
    29161 agacaagcaa gtgattgcag gtctttacag cgtgggcaat gatcgcaaga gcgtgatggg
    29221 tggcaattac ccaggcgcag gcatcacctt gggccccatc atgacttatg gctatgtcac
    29281 tgccaatgtc attgctgacc aagcacaagt acacgattta caggccgcat ctgccgccgt
    29341 ttaaacccat cgaggacaac acatgaacga acaagattac cccatcgtgg atttgcgctt
    29401 ttacagcatc gcaccgcgtg gcatggccga atttttacag catttcaacc aactggcgat
    29461 gcctttattg atgaagcact tgggcacacc tgtgggcttt tacaccacca ctgtgggcac
    29521 cttaaaccaa tttgtgcaca tttgggaata cgagagtttg gcggattatg agcgccgcag
    29581 caatgcccgc gatgccgacc cagagtttgt gacttaccta caggccacca aaggcttggt
    29641 ggtggcgcaa cacaatcaaa tcatcaaaaa agtggcgatg aacacccaaa aataagccac
    29701 acacggagaa atcagaaaca tgaagaacaa taatgtctta gacgtacgcg aggtcatcaa
    29761 tggcccgaaa atcagtggtt ttcaaaagct ggtggtattt ttttgctttg ccatcattgc
    29821 catagacggt tttgatgtgg ttgtgatggg cttggctgca ccgcaaatca tgcaagagtg
    29881 gggcgtatcg gccaaagaat tggggcctgt gttgagtgcg gctttgtttg gcttgtctat
    29941 aggcgctttg gtggcagggc ctttggcaga taagtttggt cgcaaggcgg tgttggtggc
    30001 gagtgtgcta ttttttgggg cgttcactgt gattacggcg ttttcgcaag acattaccca
    30061 cttggtggtt tatcgctttt taacgggttt gggcttgggg gcggcagcgc ctaacgcggc
    30121 cacgttgtcg gctgagtatg caccagagcg tcgccgtgct ttttttgtga ctttggcgta
    30181 ttgcggcttt tctttgggag cagcggcagg cggttttgtc gcggcgtgga tgattcctgc
    30241 attcggttgg cgcagtgtca tcctgctggg cggtgtgttg cctttggtat tgttgccgtt
    30301 catgatttgg aaaatgcctg aatcgctgtc gtttttggtg gcaaaaaatg ctgcggccgc
    30361 caaaattcgc accatcatca acaaaatcag cccccagttg gccaacgaaa acagccacta
    30421 caccgtgggc gagcctgttg ccaaggcgca aagtgccttg ggtttggtgg tgtctaagcc
    30481 gtatttcttg ggcacgatga tgttgtggct ggcctatttc acggtgttgt ttttgattta
    30541 cctgtgcagc agttggttgc ctaccatcat taaaatgaac gatttcagca tttctcaagc
    30601 cgcgaccgtg acttcggtgt ttcaaatcgg tgggccactg gggtgtttgt tctttggttg
    30661 ggcaatggac aagtttaagc cgcataaggt attggccatt gcgatgtttg tgggcgcgat
    30721 tgccacctat gccttgggtc agtttgggca caatttggtc ttgttgtcgg tgtgtgcatg
    30781 gattgttggc ttttgtttca atggcggttc ggtgggcatg tcggctttgg cgaccaattt
    30841 ttaccccacc caagcgcgag ccacgggtgc cagttggatg aatggtatcg gccgttttgg
    30901 ggcgatattg agtgcgtttg cgggcgcaca catgatcagc caaggctaca gcttgcaaac
    30961 catgtttacc ttattggtca tccctgcgct gttggcgggt ttggcggtgt tggtgaaagg
    31021 ctggaaagca cgtacggtgg ccgcgccagc attggatact gctgtggcca gataagcatc
    31081 acaccatcca tgtgtttcaa gccatggcac cacaccatgg cttttttggt ttacaggccg
    31141 cttgattggg cttttaacag catcttgccc tgtgatgatg acgccataag gtgaaagtga
    31201 aataatggct acccctcatc tcaatcagcc tgaaagcacg tcatgtggca atctttgctg
    31261 gaagcaaccc agttttccct gtctgtgacc ttaccgaatt tgctgttgtt ggggctgggt
    31321 ttgtggctgc gttaccgccg ccaagtggac aatgcgtttt gtgcccaagc gtcgaatttg
    31381 gtgttcaatt ggtcgctgcc gttgttgatg tttttcagca tcgtgggttc agatgccgat
    31441 taccgagcgc aagttgacat ggtgattgcg ggcgcattgt gtgctttggt gttgttcttt
    31501 ggttcggaat gggtggcgaa aaaaatcgtg cccgaggtgc gcgaccgtgg tgtgtttgtg
    31561 caaggggtat accgcgccaa cacaggcatc gtgggcttgg cgttttgtgc caatgcctat
    31621 ggcgctgagg gcattgccgt gggtgcggta atgacagggg cgatgacgtt tctctacaat
    31681 attttggcgg tgattacctt aacgcgctcg ctcaatcccg acacgcaaat tccgtgggcg
    31741 gccatgctca agcaaatggt gaaaaaccct ttgattgtgt ccattgtggt ggcgataggc
    31801 tggaaaagtt tggatgtgcc tttgcaccct gtatttgacc aaacaggcca ttttttggcg
    31861 gccatggctt tgcctttggc cttgatttgc gcgggtgcgg cgtttgattt gaaatcggta
    31921 ttgaaagtca gtggcgtgtc gcaatgggcg agtgtgggcc gtttgttggt cgcgccgata
    31981 gtggcggtca tcatcggcaa ggccatgggt ttggaaggca tacagtttgg cgtgttgttc
    32041 atgatgagcg ccacgcctgt ggccgccgcg agctatgtga tggtaaaagc catgggcggc
    32101 aatgatgtgg cggcggcaaa catcgtcggc atcaccacgt ttggcgcgat gtttgtggcc
    32161 gcgctgggct tggggctgat gaaacctctg ggctggttgt aaacctactg gtaaccccat
    32221 accaagcggc ctgtaaacct tgtaaatggg tttacaggcc gcttttttgt gcgcatccag
    32281 ccggggcatc ggtattgggg gctgggtgat gctgtgtgat gcctgctcaa acagttcatg
    32341 cgcatacttg ctcaacaaga taagcggcct gtaagctagg cttgctgtct ttcactcccc
    32401 aataccgccc aaaaaaggaa ccattttgaa taccttgatg tatctggctt gcatgttgat
    32461 ttggggtttg aattttatcg cggtgaaaat ccaaggcacg gaaaatccgt tggaggtgtc
    32521 gctgttgtat cgctcggcga ttgcgtttgg gctatttttt ggcttgtatt ggtggaagat
    32581 acgcccgaaa cggcaagcgc tagcgcccaa attattgggt acggtgatgg ggtttgggct
    32641 gtgcagtttt gccctcagtt atttgctgtt gtattacgcc accatataca gcagcgctgc
    32701 gatggtgacg ctgatttttt cgctcaaggt gatggtgacg ccgttgttga taggccgcgt
    32761 gttcaaaacg cccattagcc cgcgggtgta tgtgggtggg gtgtttggca ttgtgggcgt
    32821 ggcggtgata gtgttgccga atctgcatgc cttgtcacac agctttttaa tcggtttggg
    32881 tttggcggtg gcgggcactt tggtcacggc ggtgggagat acgctgtctt attacaataa
    32941 ccaacaccat gttgaaccga ttaccgccaa cagcatcggc atgctcaccg ctttactggt
    33001 gttggcggcg ctgacgtttg ccacgggcaa gcctttgcac cttcctgctt cggtgcatta
    33061 ttgggcgggg ctgctctatt tggctttgct cgg
//
LOCUS       DAOMVK010000002        22167 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_388_length_22167_cov_3.118895, whole genome shotgun sequence.
ACCESSION   DAOMVK010000002 DAOMVK010000000
VERSION     DAOMVK010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 22167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22167
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_388_length_22167_cov_3.118895"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(201..2036)
                     /gene="ggt"
                     /locus_tag="PK856_00180"
     CDS             complement(201..2036)
                     /gene="ggt"
                     /locus_tag="PK856_00180"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRL00818.1"
                     /translation="MLLKNQLWLQLGMLTFIGACTNPSTPKPNINANTPEAASGWQHK
                     DGWHTQKSAVAAANPMATEAGLKILQQGGNALDAAIAVQMVLGLVEPQSSGIGGGAFL
                     LYDDQGSITAYDGRETAPAGATENLFIGADGKPMSFDDAVVGGRSVATPAVVSMLYAA
                     HQKHGKLPWAQLFQPAIDLADKGFPVSARLNKLLKSEKFLAANDADAKAYFYQTNGEA
                     VPVGHLLKNPEYAAVLRLIAAQGPAGLNQGEVAQAMVKKVQGHANKGSLSLTDLKNYR
                     TRERTPLCFDYTARKQNYEICGMPPPSSGSLTIAQILGILAHTDAERFGLKNGALDAA
                     WLYRYGEASRLAFADRNQYIGDPDFVKAPAGSWMSLLDNGYLQQRAALIDTSAQPKSM
                     NTAKAGQPKALQAAYANMPHQKEYGTSHISIVDKYGNALAMTTTIESQFGSKLMVNRG
                     KGLAGGFLLNNEMTDFSFTPRDETGKPVANRVEPNKRPRSSMSPTLVYEKLPNGQRGK
                     LLMSLGSPGGANIIHYTAKTLYASLNWGLNAQQAINLPNFGSNNGAMQLEQGLLGDAY
                     VNALKATGQEVKTLEMTSGLQAISRTQNGWFGGADPRREGIVLGE"
     gene            2466..3362
                     /locus_tag="PK856_00185"
     CDS             2466..3362
                     /locus_tag="PK856_00185"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRL00819.1"
                     /translation="MDTIKAIQVFVEIAQQGSLTKAADKLSMSRVMVSRYLEYLENRF
                     GARLMQRNTRRVSLTQAGIEALVHCQTILEQEQMLSNLSAEIHTNGMIRVTAAHYLTR
                     EYLLDAIVAFNDIMPNVQFDVVSLENTINLVEHNIDVSIRISNKVPDGYESRDLGEMK
                     TCLVASPEYLQQCPELTTPHQLKNHRCLVHSHAAWQSWVLTNEKGQTRSFPIRSHLHA
                     NDIYVLLEMALRHQGITMLPKAMVASELATGRLVEVLPSYQLQPFQLAVVYVSSNHTP
                     LMVQNFIEFILTFFANHPHQLD"
     gene            complement(3363..4292)
                     /locus_tag="PK856_00190"
     CDS             complement(3363..4292)
                     /locus_tag="PK856_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HRL00820.1"
                     /translation="MNTVIRLSQFIQKTFALWLLLVSAVAFVYPQAFAWLTEYITWFL
                     GIIMFGMGMTMTVSDFQTLIEHPKGVIIGVLAQFLIMPTLAYVLTVVFGLPLDMAIGV
                     LLVGCCPGGTASNVITFLAKGNVGLSIACTTVSTLLAPLLTPLMFYLFANQWIDIDAS
                     SMMLSVLKVVLFPIVLGLTARTLFKQQVARSIQIMPLVSVTAIIIIVAAIVANSKAAI
                     ISSGLFLLILVVLHNGFGLLLGYFSAKLFGLPIPDRRAISIEVGMQNSGLGVALAAVH
                     FATMPVAAVPSAIFSVWHNISGSIMATWWQKRS"
     gene            complement(4401..5705)
                     /locus_tag="PK856_00195"
     CDS             complement(4401..5705)
                     /locus_tag="PK856_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003746337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRL00821.1"
                     /translation="MDLFSRQALPPLAERLRPQRLDEVIGQQHLIGAGKPLRVAVDSG
                     KPHSMLLWGPPGVGKTTLARILANSFNAQFIPLSAVLSGVKDIRTAVEQAQMALQRDQ
                     ATILFVDEVHRFNKAQQDAFLPYVENGLLTFIGATTENPSFEVNPALLSRAQVYTLKS
                     LQEADLQQLIHKTLQLPEFANLHIEADAAQALIQMADGDGRRMLNLLEQSLQAALSQN
                     LHTIDTTLLSNSLGTQLRRFDKGGDSFYEQISALHKSVRGSHPNAALYWFTRMLDGGA
                     DPRYLARRIVRMAWEDIGLADPRAMQIANDAASTYERLGSPEGELALGQAVIYLACAA
                     KSNAGYNAYNQARAWVQQEATREVPVHLRNAPTKLMKELGYGHEYRYAHNEPHAYAAG
                     ETYMPEGMEEPEFYQPVERGLEIKLKQKLEWLKSLDDEAQHN"
     gene            5996..7510
                     /locus_tag="PK856_00200"
     CDS             5996..7510
                     /locus_tag="PK856_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008775 - acetate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate CoA transferase"
                     /protein_id="HRL00822.1"
                     /translation="MSTGRIKVASLLDKVMTAAEAAQLFENGMVVGSSGFTKAGDSKE
                     VLPAFAKRAENEDIQITLMTGASLGHGTDGKLAAAGALKKRMPFQVDATLRGKINQGE
                     VLFIDQHLSEAAELLHNGTIKMDLAVLEVAGIEKDGGLIPTTSVGNSASFAALADKVI
                     LEINESVPLDVLGIHDIYVAENYPYRNVIPVVAVDNKIGRRTIPLDINKVVGIVFTSR
                     HDSPADIAAPDAKTSAIASHILNFLEQEVAEERLTERLLPLQAGIGKVANAVLTGFQH
                     SKFKHLTMFSEVLQDSTFDLIDAGIMDFASASSITVSADCYERVMGDLDKYRDKIVLR
                     PQNISNTPGLIRRLGVIAINTAIEFDIYGNVNSTHISGSKLMNGIGGSGDFARNAYLS
                     IFVTQAASKEDRISHVLPMVSHVDHTEHDVDVLVTDVGLADLRGLAPRERALEIINNC
                     VHPDYRAELLSYFERACEQTGGQTPHILSEAFSWHTRLAETGSMKQSETVSAAS"
     gene            7635..8693
                     /gene="rfaQ"
                     /locus_tag="PK856_00205"
     CDS             7635..8693
                     /gene="rfaQ"
                     /locus_tag="PK856_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipopolysaccharide heptosyltransferase
                     III"
                     /protein_id="HRL00823.1"
                     /translation="MAKRYLVIKLRHHGDVLLMTPVVHALKQREPDCEVDVLIYQETR
                     DMVAANAQIHTIHCIDRQWKKLGKRGQLAHEWDLLQAVRQRQYDAVLCLADQWRGALL
                     TRASGAKVRIGVDFKYRKGVMWRSCFTDLVAALPMSRHTVEQNLLTLEPLGIALDKNI
                     PTTMAYLPEDKEHVAKMLREQGWQGEGYVVMHPGSRWFFKCWDDDKTTTIAQRLLDAG
                     LTVVLSAAPDKRELQMLESIQKSLQTDTGRLCVLAGCMNLRELGALIDGADLVVGVDS
                     VPMHMAAAFNTPAVALFGPTWLSRWHPWSETATVVYAGDYGDLPHPDSVDVNTKTRFL
                     SAIPVEVVWRAIQTQLPQ"
     gene            8705..9127
                     /locus_tag="PK856_00210"
     CDS             8705..9127
                     /locus_tag="PK856_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003783474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00824.1"
                     /translation="MKKLMVSAVLALTLAACAGGNGGSDLGGILGGGTSTNTNNGSGG
                     AATAIGKSLIQNYAHNQCSVELNKRQEWRLMALAVSAEKQQQWEDQICGCVSEEAPNS
                     VTVTDLVQAATNQSARNTIVAGAVTKTVTSCLNRFVKK"
     gene            9138..10553
                     /gene="thrC"
                     /locus_tag="PK856_00215"
     CDS             9138..10553
                     /gene="thrC"
                     /locus_tag="PK856_00215"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRL00825.1"
                     /translation="MQYISTRGATDARSFSEVLLMGLAPDGGLMMPKTYPQVDADLLA
                     KWRTLSYAELAFEIMSLFVDDIGREDLRAMLARTYTEEVFGTADITPVRRLHDGVLVQ
                     ELSNGPTLAFKDMAMQFLGQVFEYVLARENKVLNILGATSGDTGSAAEYAMRGKQGIN
                     VFMLSPEGKMSAFQRAQMYSLADKNIHNIAVRGLFDDCQDIVKGVQADAEFKEQYAIG
                     TVNSINWARIVAQVVYYFKGYFAATQNNDEQVSFCVPSGNFGNVCAGHIARQMGLPIG
                     RLIVATNENDVLDEFFKTGAYRPRSTEHTFITSSPSMDISKASNFERFVYDLVGRDSE
                     QVKVLWAEVNAGKGFDLGFMLDKIQGEYGFVSGKSTHQNRLDTMKAVYAAGDGFIDPH
                     TADGIKVARELRMEGETIVCLETALATKFDETVHEALGKDVQIPRPANLAGLEALPQH
                     VTEVGNDVFVVKDLIRAAVKI"
     gene            10725..12467
                     /gene="msbA"
                     /locus_tag="PK856_00220"
     CDS             10725..12467
                     /gene="msbA"
                     /locus_tag="PK856_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HRL00826.1"
                     /translation="MSGWVLYRRIFQYLRNYWALFTVSIISMALAAATEPAFVALLKP
                     LVDQGFVGKNVEAMQWIPFAIIGLFLLRGVTSYINEYATAQLSGQLVLRMRTELFDKL
                     LNLPMEYFADNKSGRMISRITNDVNQISNAGFNIITVTVKDGITIMGLLGWLFYTDWQ
                     LTLITFVLLPVVAVSVRYVSKRLRSLSRRNQQYMGEMTQVLSESVHAAKVVKIYGGQE
                     QEQQRFGDSIHNVLINNVKKSVTSSLNTGITQLIIAVALSAILYFAAERARNNSFTAG
                     DFMSYLSAMLMLFAPFKRITNMSQTLQQGLASAESVFGFLDEASEPDTGSKTLAPLKQ
                     AITLEDVVFRYPNAERNAINHLNLTIEAGKIVALVGASGCGKTTLANMLSRFLIADTG
                     RIAYDGVDINDLDLKSLRSQIALVSQDVVLFNGTIMDNIAYGELRNHSREEVERAAKM
                     ANAWSFIEKLPQGLDTLIGENGTKLSGGQRQRIAIARALLKNAPILILDEATSALDNE
                     SERLVQAALEQLMQARTTIVIAHRLSTIEQADLIVVMDNGQVVEQGTHTELMQKQGRY
                     AHLHSAQLIKQTPE"
     gene            12494..13270
                     /locus_tag="PK856_00225"
     CDS             12494..13270
                     /locus_tag="PK856_00225"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HRL00827.1"
                     /translation="MAAFNSMKVLSVHHWTDAYFSFTCERDESLRFENGQFVMVGLMV
                     DGKPLMRAYSVASANWEEHLEFFSIKVQNGPLTSRLQHLKVGDEVLISKKPTGTLIAS
                     DLNAGKNLYLLSTGTGLAPFLSVTKDPDIYEQFEKVILVHGVRYKKDLAYYEHFTKEL
                     QEHEYLGDLVKEKLIYYPIVSREEFPHRGHMTDLMKSGKLFEDIGLPFISPANDRAML
                     CGGPGMLRDVSAMLDEFGLQVSPKTGVRGDYVIERAFVEK"
     gene            13339..14265
                     /locus_tag="PK856_00230"
     CDS             13339..14265
                     /locus_tag="PK856_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009118495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HRL00828.1"
                     /translation="MLDLVLWILLPLCVGFAVRISNSKQLHVINESLTIIVYLILLLI
                     GMELAQVDNLGRELGFILGVSLLFLVVNVGCNILAVMLLDIKFPLRLKTQKTQADSAK
                     HALLGSLKQIGCVAAGFIAAKLLAGWWLPPHISISIALALLLFLIGVQLRSTGINVRR
                     VFFNRRGVVLSVLLVLSSWVASVILYFGLNLFLEKPVMLSQTLAISSGFGWYSLSGII
                     MTDAYGAVWGSVALVNDLAREFVALMCIPMLMLRYPSTAVGVGGVTSLDFTLPTIQRA
                     GGVGIVPLAISFGFIMNVLSPVLMAFFAHAKF"
     gene            14356..15135
                     /locus_tag="PK856_00235"
     CDS             14356..15135
                     /locus_tag="PK856_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HRL00829.1"
                     /translation="MTLRSPRHERIVALVRDSGFMPIEELAKVLDVTPQTIRRDINQL
                     CEDNILRRYHGGAALGSSVVNDDYTARKDQNQQEKSHIAEMIAAHIPENSSLFMSIGT
                     TIEAVAQALVNTHKNLCIITNNIHVASIVSSRPDYQIMITSGVVRASDGGITGVATLD
                     FINQFKVDYAILSAASVEPDGSLLDFDYKEVSVAQAMMENARQSVLAVDHTKFGRNAL
                     VRMGDICEFNAVFTDVAPSENMQRRLEEAGVEWYLAEREPD"
     gene            complement(15432..16142)
                     /locus_tag="PK856_00240"
     CDS             complement(15432..16142)
                     /locus_tag="PK856_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00830.1"
                     /translation="MKLQTVMVLYSILMFGCSAPYEENSHSHQKSGQKALTVEAIAQA
                     KTSCLITLHKVTSRDDLIKQMFETALEDDCLYHFSPTELEKIWDIPVFDYVQVDPMML
                     PIPPNKDIESPIGLYVEKNTVPAGATFRIEMTLSELEKHFSIFPEGYFPSILPEPKSF
                     TLPEAAIRSHHVTYIPPYQSPPQAKIKADRMYYWFKDNQAVATSGFVGEITYLSFLNY
                     RSRTELSDERLAKADHSE"
     gene            complement(16503..16778)
                     /locus_tag="PK856_00245"
     CDS             complement(16503..16778)
                     /locus_tag="PK856_00245"
                     /inference="COORDINATES: protein motif:HMM:NF024712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4087 domain-containing protein"
                     /protein_id="HRL00831.1"
                     /translation="MKLDKVLGCLLGVTLVSASWAQGAIRNQTLCGWYENPTPANFYL
                     TDKSGQWVIGLQGGHQAEGDLPEFKDSQWKAYDSSRGYGYGCACIQG"
     gene            complement(17215..>17580)
                     /locus_tag="PK856_00250"
     CDS             complement(17215..>17580)
                     /locus_tag="PK856_00250"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRL00832.1"
                     /translation="AHISQFPLQIIAGHSNTDVGAVHGAAREADITQDMCNLVAHYLM
                     QTGVQIRIDGTGKGNLPLRLAVKLIKGARWPLSFIAMLLPISLQKGVEPLAHSRDKAI
                     AKTLQGSFKRDEQSIAQRG"
     assembly_gap    17581..17590
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17702..19498)
                     /locus_tag="PK856_00255"
     CDS             complement(17702..19498)
                     /locus_tag="PK856_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL00833.1"
                     /translation="MNLWQLFLKLRPFVRPYRGLVIATLFLTLIGSITAQVNALTLQY
                     AVDSINRVVEAGGGVAQGAKILIIISAILLGKEIINTAVGFGQKYYGEKLRILVSQDL
                     AQSIIEKFLRYRLAFFSDENNQAGKLQTRIDRGIASLTRLVQIFFIDILPLFTSAAIA
                     LGLMFYANVWVGLVGLLTVPVYFWITYLQAKKLGGWRRSFRDSREQKSQGILSIINSI
                     SVIKSFNREHIEAEKQLKLQHQLTLNQMHTRQLGFAFDSGKALIEQFGVVIIIILTAF
                     LVLDGQMTIGMIMYHVLLFSNVTAPIRSLHRIYDEMNDALIYSESFFNILEADDEIEP
                     TGRLKPAPLTGEFVLKNVNFNYPNGHHALKNIDMVIKPNRITALVGLSGAGKSTLISL
                     LDKFYQANSGSISVDGVDIDDIDTAYLREHIGLVLQKNHIFQGSIADNIRYGKMNATD
                     EEVMAAAEQASVHEQIMALERAYDSDATMLSGGQQQRIALARMFLKNPPIIFLDEPTA
                     SLDAIATEQIKLSLDAIKQGRTVIMISHSLSQIIDADYTYVLENGAVAEHGEHDDLYH
                     QGGVYKKIFDAMAKSLNIDKIARTFEDEDTHA"
     gene            19740..20018
                     /locus_tag="PK856_00260"
     CDS             19740..20018
                     /locus_tag="PK856_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00834.1"
                     /translation="MSDAYQSTVVATANQDNDDVMSVKEWLITLLILCVPLVNIIMMF
                     VWGFGSNTKPTKANFCKAYLILTAIFVVLYVLLIMVVGVGAAASGVSA"
     gene            complement(20123..20791)
                     /locus_tag="PK856_00265"
     CDS             complement(20123..20791)
                     /locus_tag="PK856_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRL00835.1"
                     /translation="MDHLQELLESDNKGEIIYARLANDLMNGLLLPSERLKIRDLAEK
                     MGTSVTPVRDAVLRLVQDGALIMPSPRDIRVRSLTLTEYLEIRSIRMELEGMVAAKAA
                     LLATPADIEKLEQMLVFNESALSASDSQKGLELNQSFHFELCRIAQMPILTSILHQLW
                     IKIGPLIAQSYTKGGRHMIDYHYLVVRSIREKDPQAAKIAIQTDLLSGGNVMLQLKLN
                     ESNE"
     gene            21102..22103
                     /locus_tag="PK856_00270"
     CDS             21102..22103
                     /locus_tag="PK856_00270"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRL00836.1"
                     /translation="MSILTVSPPVVTADDASKVAANLYGWHASVEALTGERDKNYLLR
                     TQEVGEVVLKFINPGEEAAETDLQTQVLLWLEQKHCAVVVPQALRNSKDGLVSDYAVD
                     EQVYKVRAYTYLNGVAVAQAQMSDALQQSFGVRAAQLVIALDGFDHPALSRVLLWDVM
                     HLNQLRDWAVEVLTDDAMGPFVFAFLDRFDATILPALQALPQQVIHGDISKSNTVVSQ
                     ADGTAIHGVLDFGDLCKAPRVVELAVTASYALDAETGDLQAALARVVSGYESILTLSE
                     AEKALIPDLIIARLVQRIVISEWRASHFPNNRDYILRSNAQARQLLGRLMQVYATNK"
BASE COUNT         5605 a         4949 c         5670 g         5933 t
ORIGIN      
        1 tttccttgtg tagaagcatt ggttttgtca tttttttgat attaataaat attttctcta
       61 agttgtcaat ctctaaatga ataaagtatt gatgcaagtc atggctgtaa tcaaaccacc
      121 tgaagagaac ttattctaat atgaatgaat gggcttaaaa aagggttggc acaggccaac
      181 ccttttgctg ttgcttggga ttattctccc aagacaatgc cttcacgacg cgggtctgcg
      241 ccaccaaacc agccgttttg ggtgcgagag atggcttgca agccgctggt catttccaag
      301 gttttgactt cttggcctgt ggccttgagt gcgttcacat acgcgtcgcc caacaagcct
      361 tgctccaact gcatggcgcc gttgttgctg ccaaagttgg gcaaattaat cgcttgttgt
      421 gcatttaagc cccaattcaa gctggcatac agggttttgg cggtgtagtg aatgatgttg
      481 gcaccgccag ggctgcccaa agacatcagc aatttgccgc gttggccatt aggtaatttt
      541 tcgtagacca aagtggggga catgctcgaa cgcgggcgtt tgttgggctc aacgcggttg
      601 gccactggct tgcctgtctc atcgcgtggg gtaaagctga agtcggtcat ttcattgttg
      661 agcaagaagc cgccagccaa tcctttgccg cgattcacca tcagtttgga gccaaattgg
      721 gattcaatgg tggtggtcat cgccaaagca ttgccgtatt tgtcgacgat gctgatgtgg
      781 gaggtgccat attctttttg atggggcatg ttggcataag cggcctgtaa cgcttttggc
      841 tggcctgcct tggcagtgtt catggatttg ggttgtgccg aggtgtcaat taaggctgcg
      901 cgttgttgca aatagccatt gtccaacaaa ctcatccatg agcctgcagg cgctttgaca
      961 aaatcggggt cgcctatgta ttggttgcgg tcggcaaagg ccaagcgcga agcttcgcca
     1021 tagcggtaca gccaagcggc atcaagagca ccatttttta gaccaaaacg ctcggcatcg
     1081 gtgtgggcca agatgcccaa gatttgggcg atggtcaaag agcctgagct cggtggcggc
     1141 atgccacaaa tttcataatt ttgtttgcgg gcagtgtagt caaaacacaa aggcgtgcgc
     1201 tcgcgtgtgc ggtagttttt caaatcggtc aaagacaggc tgcctttatt ggcatggcct
     1261 tgcacttttt tgaccatggc ttgcgccact tcgccttggt ttaaacccgc agggccttgg
     1321 gcggcaatca agcgcaatac cgcggcgtat tcagggtttt tcaataagtg acccacaggt
     1381 acggcttcgc cattggtttg gtaaaaatag gctttggcat cggcatcatt ggcggccaag
     1441 aatttttccg atttcaagag tttgttcaag cgtgcagaca caggaaagcc tttgtcggcc
     1501 aaatcgatgg cgggctgaaa caattgtgcc cacggcaatt taccgtgttt ttggtgcgcc
     1561 gcatacagca tgctgactac ggcaggcgtg gcaactgagc gaccgccgac cacggcatca
     1621 tcaaaagaca tgggcttgcc atcggcaccg atgaacaggt tttctgtagc gcctgctggc
     1681 gcggtttctc ggccatcgta agccgtgatg ctgccttggt cgtcgtaaag caagaatgca
     1741 cccccaccga tgccactgga ttgcggttct accaagccca aaaccatttg cacagcaatc
     1801 gctgcatcca aggcattgcc accttgctgc agaattttca ggccggcttc tgttgccatg
     1861 ggattggcgg cagcaaccgc tgatttttgt gtgtgccaac cgtctttgtg ttgccaaccg
     1921 ctggctgctt ctggggtgtt ggcgttgata ttgggtttgg gggtgcttgg gttggtgcat
     1981 gcgccaataa aagtcagcat gccaagctgg agccacagtt gattctttag aagcatgtca
     2041 tcctccacaa agaaagaaaa aggggtggtt gaacttaagt ttttgaattc aaagcaaggc
     2101 atttagctta ttaaactgca tggcattggt caatcaaaat ggtggcaagc ggctcaagtc
     2161 gagtagggaa aatggcattg atattgcatg cattgaaata cagtgttgct gtttgattgg
     2221 tatatgagtg cttctgtcac tcctttgtcg ccgaatatat ttaataaaag caatttgtat
     2281 tgggatggta ggcgatatct tgggtttggc tggatgttat tgatacagaa taagaagcaa
     2341 gcaaggcata tagaataaaa tactcacttt aattgtaatg ggctgaattc aaattgaatg
     2401 tttcagcggt gcttataatg acaaggtttc agggttcaag gcagtggtaa agaaagtgca
     2461 tcaatatgga tacgattaaa gcgatacagg tttttgtgga aattgctcag caaggcagct
     2521 taaccaaagc cgccgacaaa ctctcaatgt ctcgggtcat ggtgtcgcgg tatttggagt
     2581 atttggaaaa ccgttttggt gcgcgtttga tgcagcgcaa tacccgtcgg gtgtcgctca
     2641 cacaggcagg catagaggct ttggtgcatt gccaaaccat acttgaacaa gagcaaatgc
     2701 tgtcgaattt gagtgcggaa attcacacca atggcatgat aagggtcacg gcagcgcatt
     2761 acttgacacg agaatacctg ttggatgcga ttgtggcctt taacgacatc atgcccaatg
     2821 tgcaatttga tgtggtttca ttggaaaaca ccatcaatct ggtggagcac aatatcgatg
     2881 tgtcgatacg catttctaac aaagtgcctg atgggtatga gagtcgggat ttgggtgaaa
     2941 tgaaaacctg tttggtggcg agcccagaat acttacagca atgcccagaa ttgaccacac
     3001 cgcatcaatt gaaaaaccac cgttgtttgg tgcacagtca tgcggcatgg caaagctggg
     3061 tattgaccaa tgaaaaaggg cagacccgca gttttcctat ccgcagccat ttgcacgcca
     3121 atgacattta tgtgttgttg gaaatggctt tgcgccacca aggcatcacg atgttgccca
     3181 aagccatggt ggcatcagaa ttggctacag gccgtttggt agaggtgttg ccgagctatc
     3241 aattgcagcc atttcaattg gcagttgtgt atgtgtcatc caaccatacc ccgctaatgg
     3301 tgcaaaattt cattgaattc atcttgacct tctttgccaa ccatcctcat caattggatt
     3361 gattaactgc gtttttgcca ccatgttgcc attatcgagc ctgaaatatt gtgccaaaca
     3421 ctgaaaatgg cactgggtac ggcagcaaca ggcatggtgg caaaatgcac cgcggccaaa
     3481 gccacgccca agcctgagtt ttgcatgccc acttcaatcg aaatggcgcg gcgatcgggg
     3541 atgggcaagc caaacagttt ggcgctgaaa tagcccagta gcaaaccaaa cccgttgtgc
     3601 aacaccacca aaatcagcaa gaacagacca gaagaaatga tggcggcttt gctgttggcc
     3661 acgatggcgg ccacgatgat gatgatggcg gtcaccgaca ccaagggcat gatttgtatc
     3721 gagcgtgcta cttgttgctt gaacagcgtg cgcgcggtga gtcccaaaac aattgggaac
     3781 aacaccactt tcaacacaga cagcatcatg gagcttgcgt ctatgtctat ccattggttg
     3841 gcaaacaggt aaaacatcaa gggcgtgagc agcggggcca ataaggttga aaccgtggtg
     3901 caggcgatgg acaagcccac attgcctttg gccaaaaaag taatcacatt ggaagcggtg
     3961 ccaccggggc agcagcccac cagtaaaaca ccgatggcca tgtccagtgg caacccaaac
     4021 accacggtta acacataggc aagcgtgggc atgataagaa attgcgccaa cacgccaata
     4081 atcacgcctt tggggtgttc aatcagggtt tggaaatcag acaccgtcat ggtcatgccc
     4141 ataccaaaca tgatgatgcc cagaaaccaa gtgatgtatt cggtaagcca agcgaaggct
     4201 tgtggataga caaaggccac agcgctgacc agcagcagcc acagcgcaaa ggttttttgg
     4261 atgaattgac ttaggcgaat gacggtgttc acagtcaaag tcctgtaggg ctttaaagag
     4321 gcgattatac tcaggaatgt tcgcttgacc atgtttgcgt gtaataaagc ggcctgtaaa
     4381 tgtttacagg ccgcttgagt ttaattgtgc tgagcctcgt catccaagct tttcagccat
     4441 tccagttttt gcttcaattt gatttccaag ccgcgttcca cgggttggta aaattcaggc
     4501 tcttccatgc cttctggcat gtaggtttca cctgcggcat acgcatgcgg ctcgttatgg
     4561 gcatagcggt attcgtgccc ataacccaat tctttcatca atttggttgg cgcattgcgc
     4621 aaatgcactg gcacttcgcg cgtggcttct tgttgcaccc aagcacgggc ttggttgtaa
     4681 gcattgtagc ccgcgttgct tttggcggcg caagccaagt aaatcaccgc ttgccccaaa
     4741 gccaattcgc cttcgggcga ccccagcctt tcgtaagtgc tggcggcatc gttggcaatt
     4801 tgcatggcgc gcgggtcggc caagcctatg tcttcccaag ccatgcgcac gatgcgtctg
     4861 gcaagataac gtgggtcggc accgccgtct aacatgcgcg tgaaccaata caaagcggca
     4921 ttcgggtgcg agccgcgcac cgatttgtgc aaggctgaga tttgctcgta aaacgaatcg
     4981 ccgcccttgt cgaaacggcg cagctgtgtg cccaagctgt tgctgagcaa ggtggtgtct
     5041 atggtatgca gattctgact gagagcggcc tgtaaacttt gttccaacag attgagcatg
     5101 cgtctgccat cgccatcggc catttgtatc aaggcttgtg cggcgtcggc ttctatgtgc
     5161 aggtttgcaa attcaggcag ttgtaaggtt ttgtggatga gttgctgcaa atcggcctct
     5221 tgcagcgatt tgagggtata gacttgcgcg cggctgagca gggcgggatt gacttcaaac
     5281 gaggggtttt cagtggtggc gccaataaaa gtgagcaagc cattttccac ataaggcaaa
     5341 aacgcatctt gttgtgcctt gttgaaacgg tgcacttcat caacaaacaa aatcgttgct
     5401 tggtcacgtt gcaaagccat ttgtgcttgt tcaacggcgg tgcgtatgtc tttcacaccg
     5461 ctcaatactg ccgataaggg aatgaattgg gcattgaagc tgttggccaa gatgcgcgcc
     5521 aaagtggttt tgcccacacc aggcgggccc cacaacagca tcgaatgcgg tttgccactg
     5581 tctaccgcca cacgcagcgg tttgccagcg ccgattaaat gttgctgacc gatgacttca
     5641 tccaagcgct gagggcgcaa acgctctgcc aatgggggca gggcttggcg gctgaacaaa
     5701 tccatgtatc cattcccaag taaaaatgca tcatagcatt tttacaggcc gcttctgcgt
     5761 gttgcttggg ttttgagata cttaacttgt ttattgatgc tggattttat ggcgagaata
     5821 ttgcagattt ctatggtatt tatatcgaaa tataaaatta gtattgttgc atagcagcat
     5881 cattaatact tgctatatat gaatttacaa gctatgtttg catttgatta gatgaaaata
     5941 acttagataa atttcaaata aattctaagt aattaacaag ttaaaaagga acatcatgtc
     6001 aacagggcgt atcaaagtag catctctttt agacaaagtc atgacggcag ccgaagcggc
     6061 gcaattgttt gaaaacggca tggtggtcgg ctccagtggt ttcaccaaag cgggcgacag
     6121 caaggaagta ttgccagcat ttgccaaacg tgctgaaaac gaagacatac aaattaccct
     6181 gatgacaggc gcttctttgg gccacggtac cgatggcaaa ttggccgctg caggcgcttt
     6241 gaaaaaacgc atgcctttcc aagtggacgc cactttgcgc ggcaaaatca accaaggcga
     6301 ggtgttgttc atagaccaac atttgagtga agcggctgag ttgttacaca acggcaccat
     6361 caaaatggat ttggcggtgt tggaagtggc cggtattgaa aaagacggcg gtttgattcc
     6421 caccacatcc gtgggcaaca gtgcgagctt cgcggccttg gccgacaagg tgattttgga
     6481 aatcaatgaa tccgtgcctt tggatgtgtt gggcatacac gatatttatg tggccgaaaa
     6541 ctacccttac cgcaacgtga ttcctgtggt ggctgtggac aacaagatag gccgtcgcac
     6601 cattcctttg gacatcaaca aagtcgtggg cattgtgttt accagtcgcc atgacagtcc
     6661 tgctgacatt gccgcacccg atgccaaaac ttcggccatc gccagccata ttttgaattt
     6721 cttggaacaa gaagtggcag aagagcgctt aactgaacgc ttgttgccct tacaagccgg
     6781 cataggcaag gtggccaatg cggtattgac gggcttccaa cactctaaat tcaaacacct
     6841 gaccatgttc tcagaagtgt tgcaagactc aacctttgat ttgatagacg cgggcatcat
     6901 ggattttgcg tctgcgtctt caatcaccgt atctgccgat tgctatgagc gcgtgatggg
     6961 tgacttggac aaataccgcg ataaaatcgt gttacgtccg caaaacattt ccaatacccc
     7021 aggtttgata cgccgtttgg gcgtgattgc catcaatacc gcaattgaat tcgacattta
     7081 cggtaacgtc aactcgactc acatcagtgg cagcaagttg atgaatggca tcggaggttc
     7141 aggcgatttc gcgcgcaatg cttatttgtc gattttcgtg acccaagctg cgtctaaaga
     7201 agaccgcatt tcgcatgtct tgcctatggt gtcgcacgta gatcacactg aacacgatgt
     7261 ggatgtgttg gtgaccgatg tgggcttggc cgatttgcgc ggcttggcgc cacgcgaacg
     7321 cgcattggaa atcatcaaca actgtgtgca ccctgactac cgcgctgaat tgttgtctta
     7381 ttttgaacgt gcttgtgagc aaacgggcgg ccaaacgcca cacattttga gcgaagcctt
     7441 ctcgtggcac acacgcttgg ctgaaacagg cagcatgaaa caaagcgaaa cggtgagtgc
     7501 agcctcgtaa attctttgca gcattcagaa tgcggcctgt taagtgtaca ggccgcattt
     7561 ttattgcgcg tgagcggtgc ggtgatatca gttttgggta taatcgggac tttatttgaa
     7621 ttcggtggta aggcatggca aaacggtatt tggtcatcaa actgcgccac catggcgatg
     7681 tgttgctgat gacgcctgtg gtgcatgcct taaaacagcg cgagcccgat tgtgaagtcg
     7741 atgtgctgat ttaccaagaa acccgtgaca tggtggcggc gaatgcgcag atacacacca
     7801 ttcattgcat agacaggcag tggaagaaac tgggcaaacg tgggcaattg gctcatgaat
     7861 gggacttgtt gcaagcagtg cgccagcgcc aatacgatgc ggtattatgt ttggcagatc
     7921 aatggcgcgg cgctttgctc acccgtgcca gtggtgccaa agtgcgcata ggcgtggatt
     7981 ttaaataccg caagggcgtg atgtggcgga gctgttttac cgatttggtg gcagcattgc
     8041 ccatgagcag gcacacagtt gaacaaaatt tgctgacgct cgaacctttg ggcattgctc
     8101 tggacaaaaa catacccacg accatggctt acctgcctga agacaaggaa catgtggcta
     8161 aaatgctgcg cgaacaaggt tggcaaggcg agggttatgt ggtgatgcat ccaggttcac
     8221 gttggttttt caaatgttgg gacgatgaca aaaccaccac gattgcccaa cgtttgttgg
     8281 atgcaggttt gaccgtggtg ctcagtgcgg cgccagataa gcgtgaattg cagatgctcg
     8341 aaagcataca aaagtctttg caaaccgata caggccgctt gtgtgtgttg gcaggttgca
     8401 tgaatttgcg tgaattgggt gctttgattg atggtgctga cttggttgta ggggtggatt
     8461 ccgtccccat gcacatggca gcagctttca atactccagc tgtagctttg tttgggccga
     8521 cttggttgag tcgctggcac ccttggtcgg aaacggccac tgtggtgtat gctggcgatt
     8581 atggcgattt gccccatcct gacagtgtgg acgtcaacac caaaacccgt tttttgtctg
     8641 ccatacctgt ggaagtggtg tggcgagcga tacaaacaca attaccccaa tgaggagaat
     8701 cggtatgaaa aaattaatgg tcagcgcagt gcttgctttg acattggctg cgtgtgcagg
     8761 tggaaatggc ggcagtgatt tgggcggtat cttgggtggt ggaaccagta caaatacaaa
     8821 taatggctca ggtggcgcag ccactgccat aggcaaaagc ctgatacaaa actacgccca
     8881 caaccaatgc tcagtggaat tgaacaaacg ccaagaatgg cgcttgatgg ctttggcggt
     8941 gagcgctgag aaacaacaac aatgggaaga ccaaatctgt ggctgcgtgt cagaagaagc
     9001 ccctaacagt gtgaccgtga ccgatttggt gcaagcggca accaatcaaa gtgcccgcaa
     9061 caccatcgtg gccggcgccg tgaccaaaac cgtgacttct tgcttgaacc gtttcgttaa
     9121 aaaatagaaa gcctatcatg caatacatta gtacccgtgg cgcgaccgat gcccgttctt
     9181 tcagtgaagt gttgttgatg ggcttggcgc cagacggtgg cttgatgatg ccaaaaacct
     9241 acccccaagt ggatgcagac ttgttggcca agtggcgtac cttgtcttat gccgaattgg
     9301 catttgaaat catgagcttg tttgtggatg acattgggcg cgaagatttg cgcgccatgt
     9361 tggcgcgcac ttacacagaa gaggtgtttg gtactgctga catcacgcct gtgcgccgct
     9421 tgcatgatgg cgtgttggtg caagaattgt ccaatggccc aaccttggca ttcaaagaca
     9481 tggcgatgca gtttttgggt caggtgtttg agtatgtgtt ggcgcgtgaa aacaaagtct
     9541 tgaacatttt gggcgcgacc agtggcgaca caggttctgc ggccgagtat gcgatgcgtg
     9601 gcaaacaagg cattaatgtg ttcatgttgt cgcctgaagg caagatgagc gcgttccaac
     9661 gtgcgcaaat gtacagcttg gccgataaaa acatccacaa tattgcggtg cgtggtttgt
     9721 ttgacgattg ccaagacata gtcaaaggcg tacaggccga tgccgaattc aaagagcaat
     9781 atgccatcgg tacggtgaat tcaataaact gggcgcgcat cgtcgcgcaa gtggtttatt
     9841 acttcaaagg ctatttcgct gctacccaaa acaatgacga gcaagtgtcg ttctgcgtgc
     9901 catcgggcaa ttttggcaat gtgtgcgccg gccacatcgc gcgtcaaatg ggtttgccca
     9961 tcggccgttt aattgtagcc accaatgaaa atgatgtgtt ggatgagttc ttcaaaaccg
    10021 gcgcttaccg tccacgctct acagaacaca ctttcatcac atctagccct tctatggaca
    10081 tttccaaagc ctcgaatttt gagcgttttg tgtatgattt ggttggtcgc gacagcgaac
    10141 aggtaaaagt gttgtgggcc gaagtgaatg cgggtaaagg ttttgacttg ggctttatgt
    10201 tggacaaaat ccaaggggaa tacggttttg tttcaggcaa gtctacccat caaaaccgtt
    10261 tggacacgat gaaagccgtg tatgccgctg gcgatggttt cattgatccg catactgctg
    10321 acggcattaa agtggcacgt gagctgcgca tggagggtga aaccatcgtg tgtttagaaa
    10381 ccgcgctcgc caccaagttt gatgaaaccg tgcacgaggc tttgggcaaa gacgtgcaaa
    10441 ttccacgccc cgccaatttg gcgggcttgg aagctttgcc acaacacgtg actgaagtgg
    10501 gcaacgatgt gtttgtggtg aaagacttga tacgcgctgc tgtgaagatt tgatgcaagt
    10561 tgattgttaa aaccattggt aaaaagcggc ctgtaagctt acaggccgct tttgcgtcta
    10621 tgcagcttga ttgttcttaa gcctgatttc gcttacaatg agccccttta tttgagttgg
    10681 actgtcgcca ctgtatggcc tcatctgcta aatctgaaat tcctatgtct ggctgggttt
    10741 tgtatcgccg catatttcaa tacctacgca attattgggc attgttcact gtgtccatca
    10801 tctctatggc tttggctgcc gcaaccgagc ccgcttttgt tgccttgctc aaacccttgg
    10861 tggaccaagg ctttgtgggc aaaaacgtcg aagccatgca atggatcccg tttgccatca
    10921 tcggcctgtt cttgctgcgg ggggtgacca gctacatcaa tgaatatgcc accgcacaat
    10981 tgtcagggca attggtgttg cgcatgcgta ccgagctatt cgataaactt ttgaatttgc
    11041 ccatggagta ttttgccgac aataaaagtg ggcgcatgat ttcgcgcatc accaatgatg
    11101 tcaatcaaat ttccaatgct ggctttaaca tcatcaccgt caccgtgaaa gatggcatca
    11161 ccatcatggg tttgctcggt tggctgtttt acaccgattg gcaattgacc ctgattacgt
    11221 ttgtgctgtt gcctgttgtg gcggtatcgg tgcgctatgt cagcaaacgt ttgcgcagtt
    11281 tgtcacgccg caaccagcaa tacatgggtg aaatgaccca agtattgagc gagtctgtgc
    11341 atgcggccaa agtagtgaaa atttacggcg gacaagagca ggagcagcaa cgatttggcg
    11401 attcaattca caatgtgttg attaacaatg tcaaaaaaag cgtgacctca tcgctaaaca
    11461 ctggcatcac acaattgatt attgcggttg ccttgtcggc cattttgtat tttgccgccg
    11521 agcgtgcgcg taacaactcg ttcacggcag gcgacttcat gtcgtatttg tcagccatgt
    11581 tgatgttgtt tgccccattc aaacgaatca ccaatatgtc acaaacctta cagcaaggct
    11641 tggcttcggc tgagagtgtg tttggtttct tggacgaggc ttcggagcca gacacaggca
    11701 gcaaaacttt ggcgccactg aagcaagcga ttaccttgga agatgtggta ttccgttatc
    11761 cgaatgctga acgcaatgca atcaatcatt taaacctcac catagaggct ggcaaaattg
    11821 tggctttggt gggtgcttcg gggtgtggca aaaccacctt ggccaatatg ttatcgcgct
    11881 ttttaatcgc cgatacaggc cgcattgctt acgatggtgt cgacatcaac gatttggatt
    11941 tgaagagctt gcgcagccaa atcgccttgg tcagtcaaga cgtggtgttg ttcaatggca
    12001 ccatcatgga caacattgcc tatggtgaat tgcgcaatca cagccgtgaa gaagtagagc
    12061 gggcggcaaa aatggccaat gcttggtcgt ttattgaaaa actgccccaa ggtttggaca
    12121 cattgatagg cgagaacggc accaaactgt ctggcggcca acgtcagcgc atcgccatcg
    12181 cgcgtgcgct gctcaaaaat gccccgattt tgattttaga tgaagcgacc agtgccttag
    12241 acaatgagtc tgagcgtttg gtacaggccg ctttagaaca acttatgcaa gcgcgtacca
    12301 ccattgtgat tgcccaccgt ttgtctacca tcgaacaagc cgatttgatt gtggtgatgg
    12361 acaatgggca agtggtggag caaggcaccc ataccgagtt aatgcaaaaa cagggtcgct
    12421 atgcacactt gcactcggca caactgatta aacaaacacc cgagtgatgg attgatattt
    12481 aaggaaataa aacatggcag catttaacag catgaaagtc ttgtctgtac accactggac
    12541 tgatgcgtat ttttcattta cttgcgagcg cgatgagagc ttgcgctttg aaaacggcca
    12601 gtttgtgatg gttggtttga tggtggatgg taagcctttg atgcgcgcct acagtgtggc
    12661 ttcggcaaac tgggaagagc atttggagtt tttcagtatc aaggttcaaa atggtccatt
    12721 gaccagtcgc ttgcaacact taaaagtggg cgatgaggta ttaatcagca aaaagcctac
    12781 gggcacattg attgccagtg atttgaatgc aggcaaaaac ctgtatttgt tgtcgactgg
    12841 tacgggtttg gcgccattct tgagcgtgac caaagaccct gatatctatg agcagtttga
    12901 aaaagtgatt ttggtacatg gcgtgcgcta caaaaaagac ttggcgtatt acgagcactt
    12961 caccaaagaa ttgcaagaac acgaatattt gggcgatttg gtcaaagaaa aattgattta
    13021 ttaccccatc gtctcacgtg aagaattccc acaccgcggt cacatgaccg atttgatgaa
    13081 atcaggcaaa ttgtttgaag acataggctt gccattcatt agccccgcga acgaccgtgc
    13141 catgttgtgt ggtggccctg gcatgttgcg tgatgtcagt gcgatgctgg atgaatttgg
    13201 cttgcaagtg tctccgaaaa caggcgtgcg tggggactat gtgatagaac gtgcgtttgt
    13261 agagaagtaa acctcccaga agtgaacaac cgcggcctgt tacaggccgc ttttaacagg
    13321 tgagaaaaga gggtcaaaat gctggatttg gtgttgtgga ttttattgcc attgtgcgtg
    13381 ggttttgcgg tgcgcatcag caacagcaag caattgcatg tcatcaatga atccctcacc
    13441 atcattgttt acctcatctt gttgttgata gggatggagt tggcccaagt cgacaatctg
    13501 gggcgtgagc ttgggtttat cttgggtgtc agcctgttgt tcttggtggt caatgtcggc
    13561 tgtaatattt tggcggtgat gttgctagac atcaaattcc cgctgcgctt gaaaacccaa
    13621 aaaacccaag cggattcggc caaacatgct ttgttgggca gcttgaagca aatcggctgc
    13681 gtggcagctg gttttatcgc cgccaaattg ctggcaggtt ggtggttgcc accacacatc
    13741 agcatcagca tcgccttggc tttgttgttg ttcttaatcg gtgtgcaatt gcgcagcact
    13801 ggcatcaacg tgcgtcgggt attttttaac cgccgcggcg tggttttgtc ggtgttattg
    13861 gtgttgagct cttgggtggc cagtgtgatt ttgtattttg gcctcaatct gtttttggaa
    13921 aaacccgtga tgctcagcca aaccttggcc atcagttcag gctttggctg gtattccttg
    13981 tcaggcatca tcatgaccga tgcttatggt gcggtatggg gcagtgtggc cttggtcaac
    14041 gatttggcgc gtgagtttgt tgccttgatg tgcattccca tgttgatgtt gcgctaccca
    14101 agcacggcag tgggtgtggg cggcgtcacc agtttggact tcactttgcc gacgatacaa
    14161 cgtgctggtg gcgtcggcat cgtgcctttg gccatcagtt tcggctttat catgaatgtg
    14221 ttgtctcccg tgctgatggc atttttcgcg catgcaaaat tctaaaacaa acattttgcc
    14281 agtagtcttg cgcactatcg aacattttgg cacaatttag cctttacggt tactaccctg
    14341 aaaagtggtt tacaaatgac tttgcgttcc ccacgccatg aaagaatcgt tgccttggtg
    14401 cgcgacagtg gctttatgcc gattgaagaa ttggccaaag ttttggatgt gacaccgcag
    14461 accatccgtc gtgacatcaa tcaattgtgc gaagacaaca tcttacgccg ttaccatggt
    14521 ggtgcggctt tgggttctag cgtcgtcaac gacgattaca ccgcgcgcaa agaccaaaac
    14581 caacaggaaa aatcgcacat cgccgaaatg attgcggcgc acattcctga aaattcctct
    14641 ttgttcatga gcataggcac taccatagaa gcggtggcgc aagccttggt gaatacgcat
    14701 aaaaacctgt gcatcatcac caacaacatt catgtggcat ccatcgtgtc ttcacgccct
    14761 gattaccaaa tcatgattac ctcaggcgtg gtgcgtgcca gtgacggtgg cattacaggg
    14821 gtggcaactt tggatttcat caaccaattc aaagtggatt acgccatttt gagcgccgcc
    14881 agcgtcgagc ctgatggctc tttgctcgac ttcgattaca aagaagtgag cgtggcgcaa
    14941 gcgatgatgg aaaacgcacg ccaatcggta ttggctgtag accacaccaa attcggccgc
    15001 aatgccttgg tacgcatggg cgacatatgt gaattcaatg ccgtgttcac cgacgtggcg
    15061 ccaagtgaaa atatgcaacg tcgcttggaa gaggcgggtg tggaatggta tttggccgag
    15121 cgcgagcctg attagcgcaa tgctctggac aaggtaccaa gcggcctgta agttttacag
    15181 gccgcttatt ttatggggaa tggcatgtgt ttagttgcta tccgttgagc taatgctgct
    15241 ttgatgtact cagacatgag tggcatcaat tgaattccat ttcatatcag tattcttaat
    15301 caagctttga acagtctgta aaacaaacca aatgcaggcc actgtgcaaa tgctcaacag
    15361 gctttaataa agccttttta aatcatgtct gtttatcact tcatatagaa atagaagttt
    15421 ttctaaataa ctcattcaga atgatctgct ttagcaagtc tttcatcaga taactcggtg
    15481 cggctccgat agtttagaaa cgaaaggtat gtgatttcac ccacgaaacc ggacgttgct
    15541 accgcttgat tatctttgaa ccagtaatac attctgtccg ctttaatttt tgcttggggc
    15601 ggcgattgat agggaggaat ataggtgaca tggtgtgaac gtatagctgc ttcgggtaga
    15661 gtaaaagatt tgggctcagg taaaatactg gggaagtacc cttctggaaa aatgctgaaa
    15721 tgcttttcca gttcactcag cgtcatttct atacgaaaag ttgctcctgc tggtactgta
    15781 ttcttttcca catacaaccc aattggtgac tcaatgtctt tattaggagg tatgggtagc
    15841 atcattggat ctacttgaac gtaatcaaac acaggaatgt cccaaatttt ttccagttcg
    15901 gtcgggctga aatggtacaa gcaatcatct tctaaagcag tttcaaacat ttgcttgatt
    15961 aaatcgtctc gactggtcac tttgtgcagt gttataagac agcttgtttt ggcttgcgca
    16021 attgcttcaa cggtcaatgc cttctgaccg cttttttgat gactgtgact gttttcttca
    16081 tatggcgcag aacaaccaaa catcaaaatg ctgtaaagaa ccatcactgt ttgtagcttc
    16141 atattcatca ccttaatacg ccattgatgt tggaatgttt gtatttaaat tttttgataa
    16201 aaatgactat tttgtaaaat catcatgcag atacgcaacg gtatttaaaa ccacgaagcg
    16261 cttacatgac cataaaacgt aaagagatgt gtatgaacaa gctgagcaca tgtacaagcg
    16321 gcctgtacgt tctctgacac tttacaggcc gcttatacca agccaactgg cagtgatact
    16381 gacttggaac gctgtctttc taactaatgc atggctggca atgatttatc ttgtcgacat
    16441 gctgcgatgg ctttcacttg cgatttgttg atttccaata cttcacgcgt cgaagtattg
    16501 actcaacctt gtatgcaggc acagccgtaa ccatagcctc ttgatgaatc ataggccttc
    16561 cattgcgagt ccttaaactc tggcaaatcg ccctcagcct ggtgcccgcc ttgcaagcct
    16621 atcacccact gcccactctt atcggtcaga taaaaattgg ctggagtagg attttcatac
    16681 caaccacaca gcgtttgatt gcgtatcgca ccttgagccc agcttgcgct gaccaaggtc
    16741 accccaagca aacatcccaa aactttgtct aatttcatgt cactctccca gccctaatgg
    16801 aacatgcctt tggcagtcct tggtttgcat agaattaacc aaacaaaaga ttaatatgga
    16861 ttgcattaca atacaatttt gtacacaaat gacggctttc aaggccgatg tagaaatctt
    16921 aacttaattg attttatttg gattatattc ttgaaaacac agtcaaaacc aatctaatca
    16981 aatcacacgc cgccccaaca tcggcgcatc taaacgacca tccaagctaa gcgcattaat
    17041 ctctttcttg gaaacttaat tcgcctcatc aatcaacact tggaccactg ttttggccac
    17101 aagccatttc ttatcatatc aagtttgcag ttctgtatta ttactgataa aaaacaacta
    17161 atagatggtg ccgccatttg cacataagca aggcctgagc tctcaggctt ccactcagcc
    17221 tcgctgcgca atggattgtt catcacgctt gaaactgcct tgcaatgttt tggcgattgc
    17281 cttatcgcgt gaatgcgcca atggctcgac acctttttgc agacttattg gaagtagcat
    17341 tgcaatgaaa ctcagcggcc acctcgcacc tttgattaac ttcactgcta aacgcaatgg
    17401 taaattgcct tttcctgtgc cgtcaatgcg aatttgtacg cctgtttgca ttagatagtg
    17461 cgccaccaaa ttgcacatgt cttgtgtgat atccgcttca cgcgctgcgc catggactgc
    17521 acccacatct gtattgctgt gtcctgctat aatttgcagt gggaattgtg atatgtgggc
    17581 nnnnnnnnnn tatcacccat aaaactgtta ttaattggga ctgaatattc aaatgcggcc
    17641 tgtaaaccca tatgaaggct tacaggccgc ttgatttggg gtttaaacca gcatcaaagc
    17701 attacgcatg cgtgtcttcg tcttcaaacg tgcgggcgat tttgtcgatg ttgaggcttt
    17761 tggccatggc atcgaagatt tttttgtaca cgccaccttg gtggtacaaa tcatcgtgtt
    17821 cgccatgctc ggccactgcg ccgttttcca acacataggt gtaatcggca tcgatgattt
    17881 gcgacagact gtgcgaaatc atgatgacgg tgcggccttg tttgatggca tcgagcgata
    17941 gtttgatttg ctcagtggcg attgcatcta ggctggcagt gggttcgtcc aaaaagataa
    18001 tcggtgggtt tttcaaaaac atgcgcgcca aggcgatgcg ctgttgttgc ccacctgaca
    18061 gcatggtggc atcgctgtcg taagcgcgtt ccaatgccat gatttgctcg tgtaccgatg
    18121 cttgttcagc cgccgccatc acttcctcat cagtggcgtt cattttgcca tagcggatgt
    18181 tgtcagcaat gctgccttga aaaatgtggt tcttttgcaa gaccaagcct atgtgttcgc
    18241 gcaaataagc cgtgtctatg tcgtctatgt cgacgccatc cacgctgatt gagcccgagt
    18301 tggcttggta aaacttatcc agcaaactaa taagggtgga tttacctgca cccgataagc
    18361 ccaccaaagc cgtgatgcgg ttgggcttga tgaccatatc aatgtttttt aaggcatggt
    18421 ggccattggg gtaattgaaa ttgacatttt tcaacacaaa ttcgcctgtc agcggagcag
    18481 gttttaagcg gcctgtaggc tcgatttcat cgtctgcttc aaggatgttg aaaaagcttt
    18541 ctgaataaat caatgcgtcg ttcatctcat cgtaaatgcg gtgcaaagag cggatggggg
    18601 cggtgacatt gctgaacaac aacacgtgat acatgatcat gcctatggtc atttgcccat
    18661 cgagcaccaa aaatgcggtg aggatgatga tgatgaccac gccgaattgc tctatcaagg
    18721 ctttgccact gtcaaatgca aaacccaatt ggcgggtgtg catttggttg agtgtgagtt
    18781 ggtgctgaag cttgagttgt ttttccgctt cgatgtgctc acggttaaac gacttgatga
    18841 cggaaataga attgatgatg ctcaatatgc cttggctttt ttgctcacgg ctgtcgcgaa
    18901 agctgcgccg ccagccgccg agtttcttgg cttgcaaata ggtaatccaa aaatacacgg
    18961 gcacagtgag caagccgacc aaacccaccc acacattggc gtaaaacatc agccccaagg
    19021 ctatcgcggc actggtaaac agcggcaaga tgtcgatgaa aaagatttgt accaagcgcg
    19081 tgagtgaggc aatgccgcgg tcgatgcggg tttgtaactt gcccgcttgg ttgttttcat
    19141 cggagaagaa agccaagcgg tagcgcagga atttttcaat gatggattgc gccaaatctt
    19201 gtgacaccaa aattcgcagt ttttcaccgt agtatttttg gccgaaaccc actgccgtat
    19261 tgatgatttc tttgcccaat aagatagccg aaatgatgat gaggattttg gcaccttgtg
    19321 cgacaccacc accggcctca accacacggt taatgctgtc tacagcatat tgcaaagtta
    19381 aggcattgac ttgggcagta atagaaccta tcaaagtcag gaacagagtc gcgatgacca
    19441 gtccccgata ggggcgaaca aatgggcgca gtttgagaaa caattgccac aaattcatgc
    19501 aagtattcca aattgaaggt ggagaaacag tttaacgcaa tttttctgct gatacggtag
    19561 cgctggccaa aataaacggc aaaatgagcg atgaacgtga aattatcggg aaaactggtc
    19621 aagtgttgta attgagggaa gtttgatgca aagcaagaat atgcaacttg agtgtttatt
    19681 tcagttgtat tagtatgcga acactattgc aaagcataca acaaaataag ggaataagca
    19741 tgagcgatgc ataccagtca accgtggtag ccacagccaa tcaagacaac gacgatgtga
    19801 tgtcggtgaa agaatggttg atcactttat tgatattgtg cgtgcctttg gtcaacatca
    19861 ttatgatgtt tgtctgggga tttggcagca acaccaagcc aaccaaagcc aatttctgta
    19921 aagcgtattt gattttaaca gccatctttg ttgtgttgta tgtgctgtta atcatggttg
    19981 taggcgtagg ggcagccgcc agtggtgtga gtgcttaagc agcaataggg gaagtgaaaa
    20041 gcggcctgta aacatttaca ggccgctttt ttatgtttga ttaatagcat caaaacaatg
    20101 agtaagccga tttcaaaccg actcactcat tgctttcatt gagtttcaat tgcagcatca
    20161 cattgccgcc tgagagcaaa tcggtttgta tcgcaatttt tgcagcttga gggtctttct
    20221 ctcgaatgga acgaaccacc aagtagtgat aatcaatcat gtggcgaccg cctttggtat
    20281 agctttgggc aatcaaaggg cctatcttga tccacaactg atgcaaaatg ctggtgagta
    20341 tcggcatttg ggcaatgcga cacaattcaa aatgaaagct ttggttcaat tccaagcctt
    20401 tttgtgagtc ggatgcgctc agtgcgcttt cattgaacac cagcatttgt tccaattttt
    20461 caatgtcagc aggtgtggcc aataaggcgg ctttggctgc caccatacct tccagttcca
    20521 tgcgtatgga ccgaatttca aggtattcgg ttaaggtgag gctgcgcaca cggatgtcgc
    20581 gaggcgaggg catgatgagt gcgccgtctt gcaccaaacg cagcaccgca tcgcgtaccg
    20641 gtgtcacact ggttcccatt ttttcagcca aatcgcgtat tttcagtctt tcgctgggca
    20701 gtaacaagcc gttcatcaaa tcattggcca agcgggcata aatgatttct cctttattat
    20761 cagactctaa taactcttgt aggtgatcca tttgtctatc cctgatgtga ttaagccaat
    20821 tgctgtgatg catcatagaa aagctctaat ttaaaaattc tttaaaaaca atgatttaaa
    20881 tatttaaaat ttgattacgt tttaacaatt attgcttatg atgcatcatc gtaactatac
    20941 tgaatgtgac agtttgatgg caaccacaat tcttggcaaa actgcatgca attgaatgta
    21001 ttcataaaaa aatagatcaa tgtgcttaaa attttggctt aagaggctgg taggtgctga
    21061 tttcattgtg ttgagcgtaa atcgctattg aggaagaaga tgtgagcatt ttaacggtat
    21121 caccacctgt ggttacagca gatgatgcat caaaagtggc ggcaaacctg tatggctggc
    21181 atgcaagcgt ggaggcttta acgggcgagc gcgataaaaa ctatttgctg cgcacccaag
    21241 aagtggggga agtggtattg aagtttatca atccaggcga agaagctgct gaaacggatt
    21301 tgcaaaccca agtcttgctg tggttggaac agaaacactg tgcggtggtg gtgcctcaag
    21361 ccttgcgcaa tagcaaggat ggcttggtca gtgattatgc agtggatgag caagtttata
    21421 aagtgcgcgc ctatacctac ttaaatggcg tggctgtggc gcaagcccaa atgagcgatg
    21481 ctttacagca gtcgtttggt gtgcgtgccg cgcaattggt gattgccttg gatggttttg
    21541 accatcctgc tttgagccgt gtattgctgt gggatgtgat gcacttgaac cagttgcgtg
    21601 attgggctgt ggaggttttg actgacgatg cgatggggcc atttgtgttt gcgtttctag
    21661 accgcttcga cgccaccatt ttgccagcct tacaagcatt gccacagcaa gtcattcatg
    21721 gcgatatttc caaaagcaat acggttgtca gccaagctga tggtacggcc atacatggtg
    21781 tgctggattt tggcgacttg tgtaaggcgc cacgtgtggt ggagttggct gtgacggctt
    21841 cctatgcatt ggatgcagaa actggggatt tacaggccgc tttggcgcgt gtggtatcgg
    21901 gatacgaatc aatattgaca ttaagtgagg cagaaaaagc gctgattccc gatttgatca
    21961 tagcgcgttt ggttcagcgc atagtgattt cagaatggcg tgccagccat tttccaaaca
    22021 atcgtgacta tattttacgc agcaatgccc aagcacgtca gttgttgggc cgattgatgc
    22081 aagtgtatgc aacaaacaaa taatcaggca aaggacacag caatgctcaa ggaatcaaat
    22141 agcttggatg ccatgttggc aaaacgt
//
LOCUS       DAOMVK010000003        18779 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_541_length_18779_cov_3.739051, whole genome shotgun sequence.
ACCESSION   DAOMVK010000003 DAOMVK010000000
VERSION     DAOMVK010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 18779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18779
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_541_length_18779_cov_3.739051"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="PK856_00275"
     CDS             <1..911
                     /locus_tag="PK856_00275"
                     /inference="COORDINATES: protein motif:HMM:NF025878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRL00837.1"
                     /translation="WQQRVREVYFNLDVAFRSNTGSGRLLDQDLGPINVSCLNSSAMQ
                     YQRLKPAQHESYFLITLPLQNRIRMQQGQKDVYCERGQVLLQHANEPYVFEYGHENTL
                     WVMKVPSKLLKQHITHPENLTASVFDGNTGTGKLLAQNMVLLGQELLNGHLDSQQTAY
                     YNDFLLQLFAQVVKQDARVLLSDERSVRQAQLLKIQNFIATNLSHSELNPQYVAAACG
                     ISVRYLHSLFQDLDSTFTRYLQQQRLQAAYQCLQQQPERSVSDIVYSCGFNDQSHFTR
                     LFKLTYQTTPSALKNNSKSLPDGTNA"
     gene            1276..2256
                     /locus_tag="PK856_00280"
     CDS             1276..2256
                     /locus_tag="PK856_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HRL00838.1"
                     /translation="MNPAQYPAIGTPLFYGVFAVIVIIMIAIDMLALKKNGAHKVSIK
                     EALSWSLVWVAVSCAFAGWLYWYLNTHPEYGHAIAVEKTMEFFTGYVLEKSLAVDNIF
                     VFLMIFAAFKVEPQYQHRVLLYGVFGAIILRTIMIVIGAALVARFEWILYVFGAFLLF
                     TGIKMMLPEKEGEDDITQSFWFKWISKHIPVSKGYDGEKFFTIENGKRIATPLFLVVI
                     VIELSDVIFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADMADRFTYLKYG
                     LAGVLCFIGVKMLLLIFGIHIPIALSLGVVFSALIASVLVSLHVPPKSKS"
     gene            2384..4657
                     /gene="parC"
                     /locus_tag="PK856_00285"
     CDS             2384..4657
                     /gene="parC"
                     /locus_tag="PK856_00285"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HRL00839.1"
                     /translation="MSLTSSNEPDSIQLGQYAERAYLEYAMSVVKGRALPEVADGQKP
                     VQRRILFAMHEMGLAAGAKPVKSARVVGEILGKYHPHGDLSAYEAMVRMAQEFTLRYP
                     LIDGIGNFGSRDGDGAAAMRYTEARLTPLASLLLSELNQGTVDFVPNYDGAFNEPTFL
                     PARLPMVLLNGASGIAVGMATEIPSHNLNEVTNAAIALLKKPSMTVTELLEHIQGPDF
                     AGGAHIISTPEDIESTYTSGKGSVRVRARYEIEKLARGQWRAIVSELPPGVGAQKILA
                     EIEEQTNPKLKNGKKKLTQEQVNQKELLLSQLEYVRDESDSACPVRLVFEPKSSRIDS
                     EELMNTLLAQTSLEINVSINLVMMGMDGRPAQKNLLQIISEWLQFRVHTVTRRLEHRL
                     GQVEKRIHILEGRMIVFLNIDEVIAVIRESDEPKQDLMARFNLTDIQAEDILEIRLRQ
                     LARLEGIKLEQELSKLQEERQELQHILGDENVKRKLIISEMKADAKQFGDERRTLIQA
                     ASRASLTQTMVDEPITLIVSEKGWIRARTGHHLDLSQTSFKEGDALAQVIETRTVWPI
                     LLLDNQGRVYNIDAAELPSGRGDGAPLGSLVDLQAGAAPVCIISDTPESQYLLSNSGG
                     YGFISTLKDLLSRGRGGKACLALHDGETPLPAVACDQQTTVVLASRESRLLAYPLNEI
                     KNMSKGRGLQLIALDGSDVLQQCHSTNADKITVHSQGKRGGQHQDSIAISAITAKRGH
                     KGKLFADNQLLITHLDS"
     gene            4664..6259
                     /locus_tag="PK856_00290"
     CDS             4664..6259
                     /locus_tag="PK856_00290"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRL00840.1"
                     /translation="MQAALSTTDFDKAISRLPMMVNAARLAILVSLLTIFFMFRQSNT
                     GVVHLSFVPFVIWSVIYGMGIILSLFYPRWKPQQKNLPAFHDLFDITMMVGLMHLTGG
                     VENGFGILILPFIATSCLLSSGKHALIYAGFSSILIFLSTLVLQDVSYREWFTNDGAT
                     RDLFSAALLSICCFLVAALTSFVSMNVSRASQTVHAHEEEIVRLNHLNDLVLNRLQEA
                     VVVIDDQGSVWLYNTQASLYFPTLSLTDTTNLFMPLVQRWQSEPQIGFDVDYSLLDED
                     LHIRAKPLLNQNPALLILFMRPQQEIAAELQSNKLASLGQLTANIAHEIRNPLSAIRH
                     ANELLRESYETRPMQKLGAMIESNVQRIDQMVEEVLTLNQRDRLSAERVDLNHFIHEF
                     LQEFLLATPEAKDCIRVHSHRRHMVTVFDVNHLQQIMWNLMNNAWRHSLQDAEAIQIS
                     IKPHSRAGLLSFQVLDNGVGVAAENADKIFEPFFTTEARGTGLGLYVARTLAQANRGD
                     LNYLASAKAFELILPTQQHEHSA"
     gene            6243..7724
                     /locus_tag="PK856_00295"
     CDS             6243..7724
                     /locus_tag="PK856_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003794786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRL00841.1"
                     /translation="MNTPLNAPVLVVDDEEDLRDLIEMTLLKMGLKVDTAEGVVAAKK
                     KLDSQAYALILTDMRMPDGSGLEVVEYLQEKNLDIPIAIITAYGNADQAVMALKAGAF
                     DYIQKPVTLAQLRSLVKSALKVHEIGTAAPVVAEVTTSEPVQAASTSSEPSVSPAPAT
                     PALPQFPRLRGESHLLGEVKRMIAKLAKGMVPVHITGESGTGKEQAARSIHDLSPRKD
                     GPFIAVNCGAIPENLMESEFFGYKKGSFTGADSDREGFFQSANGGTIFLDEVADLPLT
                     MQVKLLRAIQERAVRRIGESKETPVDIRIVSATHKDLGREVEEGRFRQDLYYRLNVVS
                     ITMPALRELRDDLPMLINHLLANNPMGDDLVLSDAAKKQLLHYSYPGNFRELENILER
                     ATALAADGVIEPDDLQIHTHELIQDDEDDVDTAAVDVGDVSPLSHHFNMGQTTLQEYL
                     DEVERTILEQALTATKYNRTQAAKLLGISFRSMRYRLDRLEIN"
     gene            7913..8668
                     /locus_tag="PK856_00300"
     CDS             7913..8668
                     /locus_tag="PK856_00300"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRL00842.1"
                     /translation="MGPTVLDTLIQVETPEAIDIQLRPASMFQRIIAFSLDLLIRGLW
                     FTISTIVMAMIFGNASMAFESENFSYMAFGFLLINLFVTTWLYPVLFEVFNHGRTPGK
                     MAAKIRVLHDNGSPIGWSASMLRNLLRVFDGLPFAYAIGMVSMLLTNKGQRIGDIFAG
                     TIVVDEQTERQLGRLSYLEHIPSVNPPLLLTREEQYSIVSFTERHQRLPRVRQQELAE
                     QLCYDLQLPSDNPIETVLGMGKYYLGQQQKDAT"
     gene            8665..9663
                     /locus_tag="PK856_00305"
     CDS             8665..9663
                     /locus_tag="PK856_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003822554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HRL00843.1"
                     /translation="MRQHAFEALHQAFWQQFEQELGKLEQKRQLSTQTIAEFGPHYRT
                     LCHHLALANSRNYSAGLCRSLQNLVDRAHRQIYQRKNSIRKQFMQFVKFTLPQTFRQE
                     KAVIIWAHLLFYAPLFICLLLTWRYPDILDSIAGRGVGESMGDGYSEMAELQAKGTNR
                     PWGQNWMMFGFYIFNNISIGFQSFVGGLVFGLGAIYVAVFNGLMIGSVMGYMTTHPAG
                     PVFFSFVGAHGSFELTGIVLSVAGGLKLGYSLINPGEYTRRDALRIQGKQASALICGA
                     FLFLFIAAIIEGFWSPLTVLPLWLKYTVAVVLWLAVYAYLFLAGKPSSADRLQRTR"
     gene            9669..11189
                     /locus_tag="PK856_00310"
     CDS             9669..11189
                     /locus_tag="PK856_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00844.1"
                     /translation="MDFLQSRLKFRQRSPWEAVDLGRSLINSHLTYYMSLYAVLMLPF
                     VVMGALLAWLLDLNAWFVAWIWWLKPIYEYGVLSALSIHAFQPLPPLKTAIKTGFKLM
                     ARSCVIGDLTWRRLRPMRSFVLPIQQLEQLSGSDFRLRQREISRYGGNTATGLTLMGN
                     ALEGIWLYALMILAYWFLYGNFIQQGLVQPSHMNELGNLIDAFSDSWANEELSNFFNY
                     SALAYYLTLVFWGPYYVAGGFTLYLNARTQSEAWDIRLTWQRLSERLRSSSWCVAAAV
                     AVLLSFSVSDTAYAENLPDEQKVTQSREQVLQKDPFYHVKQETRWCWLSCDKKAPSER
                     KPIDFNPPAPVQGGAAVLQVLGYMVLAFLVIGLLFALWQWLKNRDSLENNNKPQDAPT
                     SMFGLDLRVEALPKDVSAQALSLLQSDPRGALSLLYRATLSQLINKQHLPVKQHHTEG
                     KVSLLVNQQLPMLSTYFQSLTNAWLRTAYGHQQLPLEDLQHLCGLYANHFEQRGAV"
     gene            11186..12583
                     /locus_tag="PK856_00315"
     CDS             11186..12583
                     /locus_tag="PK856_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HRL00845.1"
                     /translation="MSVTPRNNTSKILFIILGVLALIAIGVFLGFKREPYTTWKEDQS
                     KLTLNKHGHLLALSQFYQQHAPQMKQQAWRTWKADEPPFANQRQHLLFVYDNSNMTTS
                     QFNQLLAWVAQGNHVVMPVSDYVTPETDENPFEKQDDEVFPEAWNQLEVPEEQTANIA
                     AWAKLTFVKRDWEKQPAPLLPQCNDYLNQLDTAEKQIGSKTTLSTKERASLQAVCSQN
                     LSSIALPEGKTVWWLNQYGQEAGFKIEPSKNVLWQSKGAAGSQMVRIQHGQGSVVLVN
                     SMLGLSNPSDPRSPNSDLNRFDHAYLASYLAQDKQEVWFVNRVGTTVQSAPPLWKKLW
                     QFSPLFCGVLLLLFALFVWRSAHRQGVIKQLTRHEDRQLKQYLQAQGEFLWHKQQRVQ
                     VLSQLQQQLWQEWQRRIPGLSTFTKTEQLKAIQRIISAPDNDIELWLQALPAKPTTQE
                     WLAYLQAHQHIRNAT"
     gene            12580..13587
                     /locus_tag="PK856_00320"
     CDS             12580..13587
                     /locus_tag="PK856_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003822559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRL00846.1"
                     /translation="MSEFDLDLPLQTSSNPAIQQAFKAVQYLQQRLRSVIIGQEAVID
                     QVLTALLAGGHVLLEGVPGVGKTLLVRALAQTFGGDFKRLQFTPDLMPSDVTGHYMFD
                     GKNNSFILRQGPVFTNLLLADEINRAPAKTQAALLEVMQEQSVTLDGKCHDLNLPFMV
                     LATQNPIEQEGTYPLPEAQLDRFMFKVMIDYPSAQDEKALVQAVINSPDNDLLQNKQN
                     NSVTLSSNQVIGLQKVAQQITVDPQIIDYVVRLVATTRQWHGLSVGAGPRASIALISS
                     ARAHALLHGVDFVTPDHIRNLWLPTLRHRVRLSTEMEMENYHVDDVLNDIANSTNAPR
                     L"
     gene            13584..14921
                     /locus_tag="PK856_00325"
     CDS             13584..14921
                     /locus_tag="PK856_00325"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRL00847.1"
                     /translation="MRPSSRLITLWVIVILLVLFITISAKQLDEGLAWLTPVSALVVA
                     IAVGVSLLDWLVSKRSLNLNINRTMPDRFSVGRAHDIELSIVPKTRLKRPVKVVVYDL
                     YPNTWHVSHRELHLDLMPDRGTLTQYQATPVLRGDAEFLGTQYSVSSHLGLWQIFKRI
                     DAKTSVKVLPDFSRILGANLIGLQRWLELMGAKRIRRHGQGQDFHQLRDYTDGDNIRD
                     IDWKATSKMHKLITRTYQEERDQQLIFLLDCGRNMRTLSGDLSHFDHALNAMLLLSYT
                     ALKHDDAVGLITFNHPNMRYMPPRKGTAHLSRLVNTVYDVQPTQMAADYETAVNQLLQ
                     RQQRRALVVVLTQLDQEDNQHLLTQLQRLKKRFAVLVASLKQVERDHIQQQPLQSLDD
                     AHAYVGAQMYAAQEQQMLRKLTAQRVLHLNVNPQQLSTALINQYLELKRHGSW"
     gene            14908..15672
                     /locus_tag="PK856_00330"
     CDS             14908..15672
                     /locus_tag="PK856_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRL00848.1"
                     /translation="MAAGNTPLYVPNGHSLDHGLLDSYQQQFQLPIVNTSPEDGLYLQ
                     FDEAQRLSLYQAHSKGAVCVDFVTGRLDYRRQKGGGELIAKAINTKQHQHVIDATAGL
                     GRDAFILAALGCHVHLFERHPSVAALLQDGLRRALADADTCDIAQRMCLTPVSFLQAA
                     VESAQVVYLDPMYPQRQKSAAVKKEMAYFHALVGGNDDDIALLQQAARSAEKRVVVKR
                     PRLGEFLADLNPDYQYSGKSTRFDVYLPSKLQAALA"
     gene            15682..16551
                     /locus_tag="PK856_00335"
     CDS             15682..16551
                     /locus_tag="PK856_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009119766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRL00849.1"
                     /translation="MNPLSPITANQQRDLLHCLQQAFHVDLSTWQRLHLNNVPLGYLN
                     AQWQQQLQADIPELLSIDDDGYHILTRDWLHCADVLQNLGREWYDLGLFTGWRNEQFD
                     VCDPSGSPLFALERSLFRPLGMVSQAVHLNGFVVENGATQMWIGKRSPFKAVDPDKLD
                     NLVGGGVATGESLQEACMREGFEEAGLGTQLTGNQPAPRCIHSQRIVSRGLHHEHLFV
                     YDLSIPTDWTPENQDGEVASFQLMDIPSIVEAMLTGRFMNDAMLVTLDGLMQHGFITR
                     QSELGSWLLTQRC"
     gene            complement(16548..16931)
                     /gene="crcB"
                     /locus_tag="PK856_00340"
     CDS             complement(16548..16931)
                     /gene="crcB"
                     /locus_tag="PK856_00340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HRL00850.1"
                     /translation="MLNQSIWVMCGGALGAWCRFALQQKMPFQWANVTISTLGINVLG
                     GFIMGGVAAWLMGKSLADQALWRALLMSGFCGGFTTFSAFSLEMWQLLQAGKPINALA
                     MALAHVVLSLVAVMLGWVLVQGSVH"
     gene            17408..17875
                     /gene="hpaR"
                     /locus_tag="PK856_00345"
     CDS             17408..17875
                     /gene="hpaR"
                     /locus_tag="PK856_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HRL00851.1"
                     /translation="MTTKRHNSINIALIQARESMMAFFRPIFNQHNITEQQWRIVRLL
                     AENGTLDFQDLANKACILRPSLTGILTRLEKIDFVVRLKPTNDQRRVFLKLTKEGEQL
                     YTDVRRKIDEQYDVIETLMTKEKMQELSDLLDILNQIQDPKQEHTPTHAIEEE"
     gene            complement(17926..>18779)
                     /gene="fba"
                     /locus_tag="PK856_00350"
     CDS             complement(17926..>18779)
                     /gene="fba"
                     /locus_tag="PK856_00350"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HRL00852.1"
                     /translation="EFPDIPVVMHQDHGTSPVVCQRSIQLGFSSVMMDGSLGEDGKTP
                     TSYEYNTDVTRRVVAMSHACGVSVEGEIGCLGNLETGMAGEEDGIGAEGVLDHSQLLT
                     SVDEAVSFVKDTNVDALAIAIGTSHGAYKFSRKPDGDVLRIDRIKEIHERLPNTHLVM
                     HGSSSVPQEWLKIINEFGGDIGETYGVPVEEIVEGIKNGVRKVNIDTDLRLASTGAIR
                     RFLAENPSEFDPRKYFKVSMDAMKQLCIDRYLAFGCEGQASKIKPVSLEKMADAYAKV
                     QLNQIVK"
BASE COUNT         4907 a         4741 c         4325 g         4806 t
ORIGIN      
        1 tctggcaaca gcgcgtgcgc gaggtgtatt tcaatttgga tgtggcgttt cgctccaaca
       61 caggttcagg ccgcttgctc gaccaagacc tcggccccat caatgtgtcg tgcttaaatt
      121 catccgccat gcaataccaa cggctcaaac ctgcgcagca cgagtcttat ttcttgatta
      181 ccctgccctt gcaaaaccgc atacgcatgc aacaagggca aaaagacgtg tattgcgagc
      241 gcggccaagt attgctgcaa cacgccaacg aaccatatgt gtttgaatac ggccatgaaa
      301 acaccttgtg ggtgatgaaa gtaccgtcca aactgttgaa acaacacatt acccatcccg
      361 aaaatctcac ggcttcggtg tttgatggca acacaggcac aggcaaatta ttggcacaaa
      421 acatggtttt gctcgggcaa gaattgctca acggccattt ggactcgcaa caaaccgcgt
      481 attacaacga ttttttgttg caattgtttg cccaagtggt gaaacaagat gcccgcgtgc
      541 tgctcagcga tgagcgcagt gtgcgccaag cgcaattgtt gaaaatccaa aatttcatcg
      601 ccaccaacct cagtcacagt gaattgaatc cacaatacgt ggcagccgct tgcggcattt
      661 ccgtgcgcta tttgcacagc ttgttccaag atttggacag cacgtttacc cgctatttgc
      721 agcaacagcg tttacaggcc gcttaccaat gtttgcagca gcaacccgaa cgcagcgtgt
      781 cagacattgt ttacagctgc ggctttaatg accaatcgca cttcacccgc ttgttcaaac
      841 tcacttacca aaccacaccc agcgcactga aaaacaacag caaatccttg cctgatggca
      901 cgaatgcctg ataaatctgc ccaagtcgac acatttgcaa gctttaacac atgagtaaat
      961 ccattgccta actgactttt aaacattgtt tacaggccgc ttttaacact tcaaacaagc
     1021 caataatgat ttaaattcaa gccactgcag cgccattctg aagccttctc tatcccctca
     1081 tgacgaacca tgcaaaatat ttcacccact ctctgaactt tttaacacgc agcctatcta
     1141 ttcacgcttt gtctaattgg ccgtgattga gtttcatggt caggcttcag ttctgttaac
     1201 atatgcttcc tcaaaatcac agctttcgat tttgtcatca cctccataac actcttaaat
     1261 accctaggaa aatctgtgaa tccagcccag taccctgcca taggcacgcc gctgttttat
     1321 ggtgtcttcg ctgtcattgt catcatcatg attgcaatcg acatgttggc actcaaaaaa
     1381 aatggtgctc ataaagtcag catcaaagaa gccctttcgt ggtccctcgt ttgggtcgcc
     1441 gtttcctgtg cgtttgcagg gtggttgtat tggtatttga atacccatcc agagtatggc
     1501 catgccatcg cggtggaaaa aaccatggaa ttcttcaccg gctatgtgtt ggaaaaatcc
     1561 ttggctgtgg acaatatttt cgtcttcctg atgatttttg ccgcgtttaa agtggagccg
     1621 caataccaac accgtgtgct cttatatggt gtgtttggtg ccatcatttt gcgcaccatc
     1681 atgattgtca tcggcgcggc cttggtggca cgctttgaat ggattttgta tgtattcggc
     1741 gcgttcttat tgttcactgg tatcaaaatg atgttgcctg aaaaagaagg cgaagacgac
     1801 atcacccaaa gcttctggtt taaatggatt agcaaacaca ttcctgtgag caaaggctac
     1861 gacggcgaaa aattcttcac catcgaaaat ggcaaacgca tcgccacccc attgttcttg
     1921 gtggtgattg tgattgaatt gtcagacgtg atttttgcgg tagacagcat ccctgcagtg
     1981 tttgccgtga ccacagaccc attcatcgta ttaacctcca acatttttgc catcttgggc
     2041 ttgcgtgcga tgtatttctt gttggccgac atggccgacc gctttaccta tttgaaatat
     2101 ggcttggcgg gcgtgttgtg cttcattggt gtgaagatgt tattgttgat tttcggcatc
     2161 catattccca ttgccttgtc tttgggcgtg gtgttcagtg ccttaatcgc ttcagttttg
     2221 gtgtctttac acgtgccacc caaaagcaaa tcttaagctg tataccccat ctatgcggcc
     2281 tgtaaacctt acaggccgca tttttttgtg cgcacattgc aactgcacaa gcgcttgcag
     2341 atacattaca atggctgttt tccgtgattg tgaatgaaga gaaatgtctt tgacatcaag
     2401 caatgagcca gacagcatcc aattgggcca atacgctgag cgtgcttatt tagagtacgc
     2461 catgagtgtg gtcaaaggtc gcgccttgcc tgaagtggcc gatggccaaa agcccgtgca
     2521 acggcgcatc ttgttcgcca tgcacgaaat gggcttggcg gcaggggcaa aaccagtcaa
     2581 atcggcgcgt gtggtcggtg aaattttggg taaataccac ccacatggtg acttatccgc
     2641 ttacgaagcc atggtgcgta tggcgcaaga attcacgctg cgctacccct tgattgatgg
     2701 cataggcaat ttcggctcac gcgatggcga tggcgcagca gccatgcgct acaccgaggc
     2761 ccgcttaacc ccgttggcca gtttgctatt gtcagagctg aaccaaggca cggtcgattt
     2821 cgttcccaat tacgatggcg cgtttaacga gcccactttt ttacctgccc gcttgcccat
     2881 ggtcttgctc aacggtgctt ctggcatcgc ggtgggcatg gccacggaaa tcccgtctca
     2941 caacttgaac gaagtcacca acgccgccat tgccttgctg aaaaaaccca gcatgacggt
     3001 aacggaattg ttggaacata tccaaggccc tgattttgct ggcggtgcgc acatcatctc
     3061 cacacctgaa gacattgaaa gcacttacac ctcaggcaaa ggcagtgtgc gcgtgcgtgc
     3121 gcgttatgaa attgaaaaat tggcgcgtgg ccaatggcgc gccatcgtca gcgaattgcc
     3181 gcctggtgtg ggcgcgcaga aaattctggc cgaaatcgaa gaacaaacca atcccaagct
     3241 caaaaacggc aagaaaaagc taacccaaga acaagtcaat caaaaagaat tgctgttgtc
     3301 gcaattggaa tacgtgcgcg acgaatcgga cagtgcttgc cctgtgcgct tggtgtttga
     3361 acccaagtcc agccgcatcg acagcgaaga attgatgaac accttgctcg cacaaaccag
     3421 cttggaaatc aatgtttcca tcaatttggt gatgatgggc atggacggac gccctgcgca
     3481 aaagaatttg ctgcaaatca tcagtgaatg gctgcaattc cgtgttcaca ctgtcacccg
     3541 ccgcttggaa caccgtttgg gccaagtgga aaagcgcatt cacatcttag aggggcgcat
     3601 gattgtgttc ctcaacattg atgaggtgat tgcggtgata cgcgaatcgg acgagcccaa
     3661 acaagacttg atggcgcgct ttaacctgac cgacatccaa gccgaagaca ttttggaaat
     3721 tcgcttgcgc caattggcac gcttagaagg catcaaactt gagcaagaat tgagcaaatt
     3781 acaagaagag cgccaagagt tgcaacacat cttgggcgat gaaaatgtga aacgcaaact
     3841 catcatcagc gaaatgaaag ccgatgccaa acagtttggc gatgaacgcc gcactttgat
     3901 acaggccgca tctcgtgcca gtctcaccca aaccatggta gatgagccca ttaccttgat
     3961 tgtgtcggaa aaaggttgga tacgcgcgcg cacaggccac catttggatt tgagccaaac
     4021 ctcattcaaa gaaggcgatg ccttggcgca agtcatcgaa acgcgtacgg tgtggccgat
     4081 tttgttgctc gacaaccaag gccgtgttta caacatagat gcagccgaat tgccaagcgg
     4141 ccgcggcgat ggtgcaccat tgggctcttt ggtggactta caggccggtg ctgccccagt
     4201 gtgcatcatc agtgacacgc ctgagagcca atacttactc agcaacagtg gcggctatgg
     4261 cttcatcagt acgttaaaag acttgctcag ccgtggtcgc ggtggcaaag cctgcttagc
     4321 gctgcacgat ggtgaaacgc ctttgcctgc tgtggcatgt gaccaacaaa ccacagtggt
     4381 tttggccagt cgtgagagcc gcttgctggc ttaccctctg aatgaaatca agaacatgtc
     4441 caaagggcgt ggtttgcaac tgattgcgct agacggcagt gatgtgttac aacaatgtca
     4501 tagcaccaat gccgacaaaa ttacggtaca ctcgcaagga aaacgcggtg gccagcatca
     4561 agacagcatc gccatcagtg ccatcaccgc caaacgcggc cacaaaggca aactgtttgc
     4621 tgacaatcag ctcttaatca cccatctgga cagctaacca cccatgcaag ccgccctatc
     4681 caccaccgat tttgacaagg caatcagccg cttacccatg atggtcaatg cggcgcgctt
     4741 ggccatcttg gtatcgctgc tgaccatctt ttttatgttt cggcaatcca atacaggcgt
     4801 ggtgcatttg tcattcgtgc cttttgtgat ttggtcagta atttatggca tgggcatcat
     4861 cctgtcgttg ttttacccac gttggaaacc acaacaaaaa aatctgcctg cattccacga
     4921 tttgtttgac atcaccatga tggtgggctt gatgcaccta acaggcgggg tagaaaatgg
     4981 ctttggcatt ttgattttgc cgtttattgc cacatcttgt ttactcagct caggcaaaca
     5041 tgccttaatt tatgcgggct tcagctcaat tttgattttt ttgagcacac tggttttaca
     5101 agacgtctcg tatcgagaat ggtttaccaa tgacggcgcc actcgcgact tgttttctgc
     5161 tgctttattg tcaatttgct gttttttggt ggcagccctc acttcatttg tttcgatgaa
     5221 cgtcagccgc gccagccaaa ccgtgcatgc ccatgaagaa gaaatcgtgc gcttgaatca
     5281 cctcaatgat ttggtgctca accgcttaca agaagcggtg gtggtgattg atgaccaagg
     5341 cagtgtgtgg ctgtacaaca cccaagccag cctgtacttt cccaccctgt ctttaaccga
     5401 caccaccaat ttgtttatgc ctttggtaca acggtggcaa agcgagccgc aaataggctt
     5461 tgatgttgac tattctttgc tggatgagga tttgcacatt cgggccaaac ccttgttgaa
     5521 tcaaaaccca gccttattga ttttattcat gcgccctcaa caagagattg ccgccgaatt
     5581 gcagtcgaac aaattggctt ctttgggcca gctcaccgcc aacattgccc acgaaatacg
     5641 caatcctttg tcagccatcc gccatgccaa tgaattgctg cgcgaaagct acgaaaccag
     5701 acccatgcaa aaactcggtg cgatgataga aagcaatgtg caacgcatcg accaaatggt
     5761 agaagaagtc ctgactttga accaacgtga tagactgagc gctgagcgag tggatttaaa
     5821 ccatttcatc cacgaatttt tgcaagaatt tttactggcc actcctgaag ccaaagactg
     5881 tattcgggtt cacagccacc gccgccacat ggtcaccgtg tttgatgtga accacttaca
     5941 gcaaattatg tggaatttaa tgaacaacgc ttggcgccac agcctacaag atgccgaagc
     6001 aatacaaatc agcatcaaac cacactctcg tgctggcctg ctgtcttttc aggttttaga
     6061 caatggtgtg ggcgtagcag ctgaaaatgc cgataagatt ttcgagcctt ttttcaccac
     6121 cgaagcacgt ggtacaggtt tgggactgta tgtggcgcgt accctcgctc aagccaaccg
     6181 tggcgattta aactatttgg cctctgccaa agcttttgaa ctcattttac cgacacaaca
     6241 acatgaacac tccgcttaat gcccccgtat tggtcgtcga tgacgaagag gatttgcgcg
     6301 acctgattga aatgaccctg ctgaaaatgg gcttgaaagt agacaccgca gaaggtgtgg
     6361 ttgcagccaa gaaaaaattg gacagccaag cctatgcctt gattcttacc gacatgcgca
     6421 tgcccgatgg ctcaggtttg gaagtggttg agtatttaca agaaaaaaac ttggacattc
     6481 ccattgccat catcactgct tatggcaatg ccgaccaagc cgtgatggct ttgaaagcag
     6541 gggcgtttga ctacattcaa aaacccgtga ccttggccca actgcgcagc ttggtgaaat
     6601 ctgccttgaa agtgcatgag atcggtactg ctgcacctgt agtcgctgaa gtcaccacca
     6661 gcgaacccgt acaggccgca tcaaccagca gcgaaccctc tgtcagccca gcccctgcca
     6721 cgcctgcact gccccaattc ccgcgtttgc gaggtgaatc acacctattg ggtgaagtga
     6781 aacgcatgat tgccaagctg gccaaaggca tggtgcctgt gcacatcacg ggtgaatcgg
     6841 gtacaggtaa agaacaagcg gctcgcagca tacacgattt atcgccgcgc aaagatgggc
     6901 catttatcgc ggtaaactgt ggcgccatcc ctgaaaacct gatggaaagc gaatttttcg
     6961 gctacaaaaa aggctcgttc acaggcgcag acagcgaccg cgaaggcttt ttccaaagcg
     7021 ccaatggcgg cacgatattc ttggacgaag tggccgattt gccgttaacc atgcaagtga
     7081 agttattgcg cgccatccaa gagcgtgccg tacgccgcat tggtgagagc aaagaaaccc
     7141 cagtagacat ccgcattgtc agcgccaccc acaaagattt gggtcgtgaa gtggaagaag
     7201 gccgtttccg tcaagacttg tattaccgtt tgaacgtggt gtccatcacc atgcctgcct
     7261 tgcgcgaatt gcgcgacgat ttgccgatgc tgataaacca tttattggcc aacaatccca
     7321 tgggcgatga tttggtcttg tcagacgcgg ctaaaaaaca attgctgcac tacagctacc
     7381 caggcaattt ccgtgaattg gagaacatct tggagcgcgc cactgctttg gcagcggatg
     7441 gcgtgataga acccgatgat ttgcaaattc acacccatga gttgatacaa gacgatgaag
     7501 atgatgtgga cactgccgct gttgatgttg gcgatgtgtc accactgtct catcacttca
     7561 acatggggca aaccacttta caagagtact tagatgaagt ggagcgcacc atcttggagc
     7621 aagccttgac cgccaccaaa tacaaccgta cccaagctgc caagttgctt ggcatcagct
     7681 tccgttcgat gcgttaccgt ttggatcgtt tggaaatcaa ttaacacatg cctctgtgtt
     7741 acaggccgct tgtttccatt caagcggcct gtaacacaaa aagcagtatc agatgaaccc
     7801 gtaaggctta tttgctaccg cttacatgac acactgcccg tcaaacttga caccccccac
     7861 agggagtatg ctttaatcgc aattactcta acctgcctcg tgaaagtacc gtgtgggccc
     7921 tactgtttta gacactttaa ttcaagtcga aacgcccgaa gcgatagaca tacaattgcg
     7981 ccctgccagt atgtttcaac gcatcatcgc tttcagcttg gatttattga tacgcggttt
     8041 gtggttcacc atcagcacca tcgtcatggc catgattttt ggcaatgcca gtatggcttt
     8101 tgaaagtgaa aacttttcct acatggcatt tggtttcctg ctgatcaatc tgtttgtcac
     8161 cacttggctg tatcccgtgt tgtttgaagt ctttaaccat ggccgcaccc caggcaaaat
     8221 ggccgccaaa atccgcgtgt tacacgacaa tggcagcccc ataggctggt ctgcatccat
     8281 gttgcgcaat ctactgcggg tatttgatgg gctgcctttt gcctacgcca tcggcatggt
     8341 atcgatgttg ctcaccaaca aaggccaacg cataggtgac attttcgcgg gcaccatcgt
     8401 ggtagacgaa caaacagaac gccaactggg tcgcttgtct tatttggaac acattcccag
     8461 cgtcaatccc cctttgctgc tgacccgtga agaacagtat tccatcgtgt cttttaccga
     8521 gcgccaccaa cgtttgccac gggtacgcca acaagaactg gctgaacaat tgtgttacga
     8581 cttgcaatta ccatctgaca atcccattga aactgttttg ggcatgggca aatactattt
     8641 gggccaacaa caaaaggatg cgacatgagg caacacgcat tcgaagcatt gcaccaagcc
     8701 ttttggcagc agtttgaaca agaattgggc aaactggaac aaaagcgcca actcagtacc
     8761 caaaccattg cggaatttgg cccccactac cgtactctat gtcaccactt ggctttggcc
     8821 aacagccgca attactcagc aggcttgtgc cgcagcctac aaaacttggt agaccgagcc
     8881 cacagacaaa tttatcagcg taaaaattcg atacgcaaac aattcatgca gtttgtgaag
     8941 ttcaccttgc cccaaacctt tcgccaagaa aaagccgtga ttatttgggc gcacttgctg
     9001 ttttacgctc cgttgttcat ctgtttgctg ttgacttggc gctaccctga catcttggac
     9061 agcatagctg gccgcggggt gggcgaatcc atgggtgatg gctacagcga aatggccgaa
     9121 ttacaagcca agggcaccaa tcgcccttgg gggcaaaact ggatgatgtt tggcttttat
     9181 attttcaaca acatcagcat cggcttccaa agtttcgtag gcggcttggt gtttggtctg
     9241 ggtgccattt atgtcgccgt cttcaatggc ttgatgatag gctcagtgat gggctacatg
     9301 accacacacc ctgcgggccc agtatttttc tcttttgtgg gtgcacacgg ttcgtttgaa
     9361 ttgacgggca ttgtgctatc ggtagcaggc ggcctgaaac tgggttacag cttaattaat
     9421 cccggagaat atacccgtcg tgacgcctta cgcattcaag gcaaacaagc ttccgcactg
     9481 atttgcggtg ctttcttatt cttattcatc gccgccatca tcgaaggctt ctggtcacct
     9541 ttgaccgtat tgcccttatg gctcaagtac acagtggccg tggtattgtg gttggctgtg
     9601 tatgcttatt tgtttttggc tggcaaacca tcctctgccg accgtttgca acggacgagg
     9661 taaatcacat ggattttttg cagtcacgct tgaaatttcg ccaacgctcc ccttgggaag
     9721 ccgtggattt gggccgcagc ctgattaatt cccacctcac ctattacatg tctttgtatg
     9781 cggtgttgat gttgcccttt gtggtgatgg gtgccttatt ggcttggttg ttggatttaa
     9841 atgcatggtt cgtagcgtgg atttggtggc tcaagcccat ttatgaatat ggtgttttgt
     9901 ctgctttgtc gatacatgcg tttcaaccac tgcctccatt gaaaaccgcc attaagacgg
     9961 gatttaagtt gatggcacgt tcgtgcgtga taggagattt aacatggcga cgcttgcgcc
    10021 ccatgcgcag cttcgtgtta cccattcaac aattggaaca gctttcaggt agcgattttc
    10081 gcttgcgcca acgggaaatt agtcgctatg gcggcaatac cgcaacaggc ttaaccctga
    10141 tgggcaatgc cttagaaggc atttggttgt atgccttgat gattctggcg tattggtttt
    10201 tgtatggcaa tttcatacaa caaggcttgg ttcaacccag ccacatgaac gaattgggta
    10261 atctgattga tgccttcagc gacagttggg ccaatgagga actgtccaac ttcttcaatt
    10321 attctgcgct ggcgtattat ctgaccttgg ttttctgggg gccatattat gttgcaggtg
    10381 gctttacctt gtatttgaat gcacgcaccc aatctgaagc ctgggacatt cgtttgactt
    10441 ggcaacgctt aagcgaacgc ttgcgcagca gcagttggtg tgtggcggcg gccgtagccg
    10501 tgttgttatc tttctcagtt agtgacactg cttatgcgga gaacttgccc gatgagcaaa
    10561 aagtgactca aagtcgcgaa caagtcttac aaaaagaccc gttttaccat gtcaaacaag
    10621 aaacccgttg gtgctggctc agttgtgaca aaaaagcccc atctgaacgc aaacccatag
    10681 atttcaatcc gcctgcaccc gtacaaggcg gggccgcagt gttgcaagta ctgggttata
    10741 tggtgttggc tttcttggtg attggcttgc tgtttgcctt atggcagtgg ctgaaaaacc
    10801 gtgacagtct tgaaaacaac aacaaaccgc aagatgcccc cacttccatg tttggtttgg
    10861 atttgcgtgt tgaagcctta cccaaagatg tttctgctca ggctttatct ctgttgcaat
    10921 ccgacccccg aggcgcatta agcctgttat accgtgccac tttatcgcaa ttgattaaca
    10981 aacagcactt acctgtgaaa caacaccata ccgaaggcaa ggtgtcttta ctcgtcaacc
    11041 aacaattgcc catgctcagc acgtattttc aaagcctgac caatgcttgg ttgcgcacgg
    11101 cttatggtca ccagcaattg ccattagaag atttgcaaca tttatgcggc ctgtacgcca
    11161 atcactttga acaacgaggt gcggtatgag tgtgacgcca cgcaacaaca cctccaaaat
    11221 tttgttcatc attctcggtg tattggccct cattgccatc ggtgtgtttc tgggttttaa
    11281 gcgtgaaccc tataccacat ggaaagaaga ccagtccaag ctgactttga acaagcatgg
    11341 gcatttgttg gccttgagtc agttttacca acaacatgct ccacaaatga agcaacaggc
    11401 gtggcgcact tggaaagccg atgagccacc ttttgccaac caacgccaac acctgttgtt
    11461 tgtctatgac aacagtaata tgaccaccag ccaattcaat caattattgg cttgggtggc
    11521 ccaaggcaat catgtggtca tgcctgtcag tgactatgtc acacctgaaa cagatgagaa
    11581 tccatttgaa aaacaagacg atgaagtgtt tccagaagca tggaaccaat tggaagttcc
    11641 cgaagagcaa acggccaata ttgccgcatg ggccaagctg acttttgtga aacgcgattg
    11701 ggaaaaacaa cccgcgccgc ttttgcctca gtgcaatgat tatttgaatc aattggatac
    11761 tgctgaaaag caaataggca gcaaaaccac tctcagcacc aaagagcggg ccagtttaca
    11821 ggccgtttgt tcacaaaacc tcagcagcat tgcgctgcca gaaggcaaga ctgtatggtg
    11881 gctcaaccaa tacggccaag aagcaggctt taaaattgaa ccgtctaaaa acgtattgtg
    11941 gcaaagtaaa ggtgccgcag gcagtcaaat ggtgcgcatt caacatggcc aaggcagcgt
    12001 ggtattggtg aactctatgc tgggcttgag caatcccagc gacccacgtt cacctaacag
    12061 tgatttaaac cgcttcgacc atgcttattt ggcctcgtat ttggctcaag acaagcaaga
    12121 ggtttggttt gtcaatcgtg tgggcactac cgtacaaagc gcgccaccat tgtggaaaaa
    12181 actgtggcag ttttcaccct tattctgtgg cgttttattg ctgttgttcg cgttgtttgt
    12241 gtggcgatct gcccaccgtc aaggggttat caaacaactg acgcgacatg aagaccgtca
    12301 gttgaaacaa tacttgcaag cccaaggtga attcctgtgg cacaagcaac agcgcgtaca
    12361 agtgttgagc caattgcagc aacaattgtg gcaagaatgg caacgccgta tcccaggcct
    12421 aagcactttt accaaaaccg aacaattaaa agccatacag cgcatcatca gcgcgccaga
    12481 caacgacatt gaattgtggc tgcaagcctt accagccaaa ccgactaccc aagaatggct
    12541 tgcctatttg caggctcatc aacacattag gaatgccaca tgagcgaatt tgacttagac
    12601 ctgcccttac aaaccagcag caatccggcc attcaacaag catttaaagc cgttcaatac
    12661 ctgcaacagc gtttgcgctc agtcatcatc ggccaagaag ccgtcataga ccaagtgttg
    12721 actgccttac tcgcaggtgg ccatgttttg ctcgaaggcg taccaggtgt gggcaaaacc
    12781 ttattggttc gcgccttggc acaaactttt ggtggcgatt tcaagcgctt gcaattcaca
    12841 cccgatttga tgcccagtga tgtgaccggt cattacatgt ttgatggcaa aaacaacagc
    12901 tttattttgc gccaaggccc tgtgtttacc aatttactgt tggcagacga gattaaccgc
    12961 gcccctgcca aaacccaagc tgccttgcta gaagtgatgc aagagcaatc ggtaacgctg
    13021 gatggcaaat gccacgattt gaacctgccg ttcatggttt tggccaccca aaaccccatt
    13081 gaacaagaag gcacttatcc cttaccagaa gcccaattag accgcttcat gttcaaggtg
    13141 atgatagact atccgagtgc acaagatgaa aaagccttgg ttcaggccgt gattaacagc
    13201 cctgacaatg atttgctgca aaacaaacaa aacaacagcg ttaccctcag cagcaatcaa
    13261 gtcattggct tgcaaaaagt ggcgcaacaa atcactgtgg atccgcaaat catagattat
    13321 gtcgtacgtt tggtcgcaac cacccgtcaa tggcatggcc tatcggtagg tgcaggccct
    13381 cgcgccagca tcgccttaat cagcagcgcc cgtgctcacg ccttgttgca tggggttgat
    13441 tttgtcacac cagaccacat ccgcaatttg tggctgccca ctttgcgtca ccgtgtgcgt
    13501 ttgtctaccg aaatggaaat ggaaaattac cacgtcgacg atgtgttaaa tgacatagcc
    13561 aacagcacga atgccccacg cctatgagac caagttcgcg actgattacc ctttgggtca
    13621 tcgtgatttt actggtgttg ttcatcacca tcagtgccaa acagctggac gaaggcttgg
    13681 catggttgac acctgtcagt gcattggtgg ttgccatcgc agtgggcgtc agcttgctag
    13741 actggttggt gtctaaacgc agcttgaact taaacatcaa tcgcaccatg cccgaccgct
    13801 tttctgttgg cagagcgcac gacatcgaat taagcatagt gcccaaaacc cgtttaaagc
    13861 ggcctgtaaa agtggtggta tacgatttgt atcccaatac atggcatgtg tctcatcgcg
    13921 aattgcattt ggacttaatg ccagatcgtg gcaccctcac ccaataccaa gcgactcccg
    13981 tactgcgtgg tgatgccgaa tttctaggca cccaatacag tgtgtccagc catttgggtt
    14041 tgtggcagat tttcaaacgc atagacgcca agaccagcgt gaaggtattg cccgattttt
    14101 cacgcatctt gggcgcgaat ttgataggct tgcagcgttg gttggaatta atgggtgcca
    14161 aacgcatccg ccgccatggg caaggccaag attttcacca attgcgtgac tataccgatg
    14221 gcgacaacat ccgtgacata gactggaaag ccacctccaa aatgcacaag ttaatcacgc
    14281 gcacttacca agaagagcgt gatcagcaat tgattttctt attggattgt ggccgcaaca
    14341 tgcgtacttt aagtggggat ttgagccatt tcgaccatgc attgaatgcc atgttgcttt
    14401 tgagttatac cgccttaaaa cacgatgatg cagtgggatt gattacgttt aaccacccca
    14461 atatgcgcta catgccacca cgcaaaggca cggcgcactt gagtcgcttg gtcaacacgg
    14521 tgtatgacgt acagcccact caaatggctg ccgactatga aaccgccgtt aatcaattgt
    14581 tacaacgcca acaacgtcgg gccttggtgg tggttttgac ccagttggat caagaagaca
    14641 accaacattt gttaacccaa ttgcaacggt tgaaaaaacg ctttgcggta ttggtggcca
    14701 gtttaaaaca ggtggaacgc gatcacatac aacagcaacc cttgcaaagt cttgatgatg
    14761 ctcatgctta tgtgggggca caaatgtatg cggcccaaga acaacaaatg ctgcgcaagc
    14821 tcaccgcaca acgtgtgtta cacttgaacg tgaatccgca acaattgagc accgctttga
    14881 tcaatcaata cctcgaatta aaacgccatg gcagctggta atactccttt gtatgtgcct
    14941 aatgggcaca gcctagacca tggtttgtta gactcttatc agcaacaatt tcaactgccc
    15001 attgtcaata ccagcccaga agacggcctg tatttacaat ttgatgaagc acaaagactc
    15061 agcttgtacc aagcacacag caaaggcgct gtgtgtgtgg attttgtgac gggtcgcttg
    15121 gattaccgca gacaaaaagg cggcggtgaa ttgattgcca aagccatcaa caccaaacaa
    15181 caccaacacg tcatcgatgc cacggcaggc ttgggacgcg atgccttcat tttggccgca
    15241 ttgggctgcc atgttcacct gtttgagcgc cacccaagcg tcgctgctct attacaagat
    15301 ggtttgcgcc gcgccttggc cgatgctgat acctgcgaca tcgcccaacg catgtgctta
    15361 acccctgttt catttttaca ggccgctgtt gagtcggcgc aagttgttta tcttgacccc
    15421 atgtatcctc aacgtcaaaa atcggcagct gtcaaaaaag aaatggcgta tttccacgct
    15481 ttggtgggtg gtaacgacga tgatattgcc ttattgcagc aagccgcacg cagtgccgaa
    15541 aaacgcgtgg tggtaaaacg tccacgcttg ggtgaatttt tagcggattt aaatcctgac
    15601 taccaataca gtggcaaatc aacccgattt gatgtttacc ttccctccaa gttacaggcc
    15661 gctttggctt aaggacgaag tttgaatccc ttatccccca ttactgccaa ccaacaacgt
    15721 gatttattgc actgcttaca acaggctttt cacgttgatt tgagcacttg gcaacgcctg
    15781 catttgaaca atgtaccttt gggttatcta aatgcccaat ggcagcaaca gttacaggcc
    15841 gatattccag agctattaag catagatgac gatggttacc acatccttac ccgtgattgg
    15901 ctgcactgtg ccgatgtgct gcagaatttg gggcgcgagt ggtacgactt gggcttgttt
    15961 acaggttggc gcaatgagca gtttgatgtg tgcgaccctt caggctcgcc cttatttgca
    16021 ttggagcgct cattatttcg cccattgggc atggtcagtc aagccgtaca cttgaatggt
    16081 tttgtggtag aaaacggtgc aacccaaatg tggataggca aacgcagccc cttcaaggct
    16141 gttgatcctg acaaattgga caatttggtc ggcggcggcg tggccacagg tgaaagtctg
    16201 caagaggcat gtatgcgtga gggctttgaa gaggctggct tgggtacaca gctgacaggc
    16261 aaccaacctg caccccgttg catccacagt caacgcatcg tcagccgtgg tttgcaccat
    16321 gaacacttgt ttgtctatga tttatctatc ccaacagact ggacaccaga aaaccaagac
    16381 ggtgaagtgg cttcatttca attgatggac attccaagca tcgtagaagc catgcttaca
    16441 ggccgcttta tgaacgatgc catgttggtg acattggatg gcttaatgca acatgggttt
    16501 attacccgcc agagtgaatt gggttcatgg ctgttgactc agcgttgcta atgcacactg
    16561 ccttgcacca acacccatcc caacatcacc gccaccaaag acaaaacgac atgtgccaaa
    16621 gccatcgcta gggcattgat gggtttgccg gcttgtaaca attgccacat ttccaaagaa
    16681 aatgcagaaa acgtggtaaa tccaccgcaa aatccactca tcaatagcgc ccgccaaagt
    16741 gcttgatccg caagcgattt acccatcaac caggccgcca ctccacccat gatgaagccc
    16801 ccaagcacat taatgcctaa agtagaaatc gtcacattgg cccactgaaa tggcattttt
    16861 tgctgcaacg caaaacgaca ccatgccccc aaggccccac cacacatcac ccaaatcgat
    16921 tgatttaaca tccttaaaac ccgcatccta attttgcaca gactttaaac cgaaaccatt
    16981 gttctgttaa gtgcttacac tgtttttctg aatacaaaaa agatttcatt aacttattac
    17041 attgaaattt ctctgcctca ttttggcttt tgaacagcga tgatttttta atgtattgtc
    17101 gatagtacat gccaaattta tgcatatagt ataatttatt ttatgcacat agtaacattt
    17161 attgagcaca tatcctgctg atatggctac aatcacattt ctcaaagcaa gcagcacaag
    17221 acagtttaca taaaacgtaa atgaaggtat tttgtttgtc agcaggaact tagtcctgat
    17281 ttgacatctg tagatgacac aaggttttta ttcatactta aacgttgcga aagcaattgt
    17341 atactgatag tatttgacga cacatgtact tgagtgaatc gcaaattcaa acacaggaac
    17401 gctcacaatg acgaccaaac gacacaactc catcaatatt gcgttgattc aagcacgtga
    17461 atccatgatg gctttttttc gccccatttt caaccaacac aatatcactg aacaacaatg
    17521 gcgcatcgtg cgtttattgg cagaaaatgg cacgttggat tttcaagatt tggccaacaa
    17581 agcctgcatc ttacgcccta gtctcactgg cattttgacc cgtttggaaa aaatcgattt
    17641 tgtggtgcgc ttaaagccca ccaacgacca gcgccgtgtt tttttgaaat tgaccaaaga
    17701 aggtgagcaa ctgtataccg atgtgcgtcg caaaattgac gagcaatacg atgtcattga
    17761 aacgctgatg accaaagaaa aaatgcaaga gttgtcggat cttttagaca ttttgaatca
    17821 aattcaagat cccaagcagg aacacacccc aacccatgca atcgaagaag aatgataaaa
    17881 aggcgccctt tacaggacgc cttttttaac atttcatcgc attgattatt ttacaatttg
    17941 attcaattga actttggcat aggcatcggc cattttttcc aatgaaacag gtttgatttt
    18001 gctggcttgg ccttcgcaac caaatgccaa ataacggtca atacacaatt gtttcatggc
    18061 atccatggat actttgaagt atttacgtgg atcaaactct gaaggatttt cagccaagaa
    18121 acggcgaatc gcacctgtag atgccaaacg caaatcggta tcaatgttga ccttgcgcac
    18181 accattttta atgccttcaa cgatttcttc tactggcaca ccataagttt cgccaatgtc
    18241 accaccaaac tcattgatga ttttcaacca ttcttgaggc accgaacttg agccgtgcat
    18301 caccaaatgg gtattaggca aacgttcatg aatttctttg atgcgatcaa tgcgcagcac
    18361 gtcaccatca ggcttgcggc tgaatttgta agctccgtgg ctggtaccga tggcaatggc
    18421 caaagcatcc acattggtgt ctttcacaaa tgacactgct tcgtctacac tggtcaataa
    18481 ctgactgtga tccaacaccc cttcagcacc gatgccatct tcctcccccg ccattcctgt
    18541 ttctaggttg cccaaacaac caatttctcc ttcaacagag acaccacaag cgtgcgacat
    18601 tgcgacaacg cgacgggtaa catcggtatt gtattcatag gaagtagggg ttttaccgtc
    18661 ttcacccaat gagccatcca tcatcacaga actaaagccc aattggatag aacgctgaca
    18721 aacaacaggg ctggtgccat ggtcttggtg catgaccaca ggaatatcag ggaattctt
//
LOCUS       DAOMVK010000004        17344 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_638_length_17344_cov_3.279773, whole genome shotgun sequence.
ACCESSION   DAOMVK010000004 DAOMVK010000000
VERSION     DAOMVK010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 17344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17344
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_638_length_17344_cov_3.279773"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2256
                     /locus_tag="PK856_00355"
     CDS             <1..2256
                     /locus_tag="PK856_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HRL00853.1"
                     /translation="AIGLLDEGATVPFIARYRKEVTGGLDDTQLRNLAERLQYLRELI
                     DRKATILKSIDEQGKLSPELAAQIEAIDNKTALEDVYLPYKPKRRTKAQIAREAGLEP
                     LAETLWQTPNTDPETEAAKYLNAEHNIADSKAALDGARAILMEQFAEDAILLGQLRER
                     LWQVGDIRSEVVAGQEQTGEKFQDYFKHQEALRSLPSHRSLALLRGRNEGVLALKIVY
                     QADDTPITEASEFEQTIAAHYGIADQKRTADKWLKDTVRLTWRAKLFLSLELDAFNRI
                     KTEGDAAAITVFARNLKDLLLAAPAGRLVTLGLDPGIRTGVKVAVVDDTGKLLATDTV
                     YPFQPRNDKHGAMASLARLIKQHGVQLIAIGNGTASRETDQLAAEVMKAMPEAGVHKL
                     VVSEAGASVYSASELAANEFPDLDVSLRGAVSIARRLQDPLAELVKIDPKSIGVGQYQ
                     HDVNQVQLAHSLDAVVEDCVNAVGVDVNTASAPLLARVSGLNATLAHNIVAYRDENGA
                     FKNRKQLKKVPRLGDKTFEQAAGFLRIQGGTEPLDASSVHPEAYPIVAKMLADTGSSA
                     DKLIGNMPVLKTLQAARYIDAQFGLPTVMDILSELEKPGRDPRGEFKTATFADGIDDI
                     KHLVVGSIMEGVVTNVAAFGAFVDVGVHQDGLVHISALSKQYVSDPHTVVKAGQVVKV
                     KVLSVDVARKRIALTMRLDDDIETGSQRGNANNSAGNHQRGERRGGNERKREEAPVNN
                     AMADAFAKLKR"
     gene            complement(2657..3184)
                     /locus_tag="PK856_00360"
     CDS             complement(2657..3184)
                     /locus_tag="PK856_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HRL00854.1"
                     /translation="MFEHKIIFTGPVGAGKTTLIKAFSDDAPILTEVRPSDDVANMKD
                     QTTVAMDYGSVVLDEETKVHMYGTPGQQRFDFMWEILSKGSMGLAILINAAATDPAAD
                     LRFYLNAFKGLIVDENIPLVIGINRVDKSNPNHPLEDFQEVADEFRDEIPVLMVDVRE
                     SRDVRRLVMTLLFTI"
     gene            3865..5544
                     /locus_tag="PK856_00365"
     CDS             3865..5544
                     /locus_tag="PK856_00365"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL00855.1"
                     /translation="MKIKKLWQTPPVTYTFKSHEQPFMIGSPGVPDHPTRRKVFYTLV
                     GIVLALTGGMQNGLLIAGLPQLQGALALTPTEGGWILVAYNMVNACMSMLLFKCRQQF
                     GLARFIRAIIMALVLCNLIQVATHSYHSELVARGISGLAASGMNTLALFYFIQGMPTK
                     WRLKGIACSISVSQVALPFARAIAPALMNDGNIQAVFWLQLGLSLNVLVLVSLLPLPH
                     GYTQKAISKTDLFSLLCFAFGVAALCAFFVQGRIIWWDKIVLGQLLLVALVGLGITLL
                     IESNRQDPMIKLDWLRSKELLWLGLTAAFLRMVMSEQNVGSAGLLTALGMGNTQMVTY
                     YWVLTAGALTGLAATLWLMEPQNLKPPVMLAFFCIAVGAFLDVGANSLTRPSQTYVSQ
                     FLIAMASVCFMAPLMIEGMLRAFSKSPAHIITFIAVFGLSQTIGGLFGSAALSTFLTL
                     RTKEHLTRFGEQLSMTDPTVAARVQAYAAQYQGVIGDGALRQGQGVAGVVAQAQQQAT
                     VLAYNDSFWLFGLVASCGFLFVSAEWAYYKYHGTSHMAPVLAELAKMKAKK"
     gene            5560..6759
                     /locus_tag="PK856_00370"
     CDS             5560..6759
                     /locus_tag="PK856_00370"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HRL00856.1"
                     /translation="MAESTKPEEQRPVNAETPSETKQEAETPAANAQAAEPAAHDTKT
                     TVTVADNWHPPKKTHLKNAVLIAVGLVAIALVLYAWKLPPFQSAVVSTDNAYVRGQTT
                     VISPQVTGYVTRVYVQDFEDVAAGTPLVQIDPRIYQQKVEQAHAQVMAAESNLHNNQQ
                     SLTSSQSTVLARNASIANAEAMLKKAQADMARVKELVGLGSLSPREGDQARAALQQAQ
                     AAVAEARAQKAVAEQGVKSTHVGKQGLQAAVAQAEAQAQLAQIDLDNTTIKAPQNGRL
                     SEIGVKPGQLVNAGTQLMYLVPSHVWVVANFKETQTANIRVGQNAKVRVDALGGAELT
                     GKVERIAPAAGSEFSVIRPDNATGNFVKVAQRIAVRIQINPNQELAKRLSPGMSVEAS
                     VDTGSKS"
     gene            6756..8156
                     /locus_tag="PK856_00375"
     CDS             6756..8156
                     /locus_tag="PK856_00375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HRL00857.1"
                     /translation="MRQVWFLSMVLGLSACVPKIPPQPSDAALNPPEQWLQAVAINHM
                     GLGVTLAEPQTWWAQLGDAQLNQYVAQALRYNSDVNIAATRVQAAMAQEAVNRSVLFP
                     NLSVGGAGSNGRSVNAFGVPSQSAVAQPVFQASYEVDVFGKNRHAWRAKKWQTAAAEA
                     QQAATQVSVSTAVARLYVQLTALQAQKQLLQNTLRSREQALKIAQDKARVGYSSQLEL
                     NQAQAEYAATKQQIPINQKAISQSQHALSTLIGSVPQALPAADSLKSLHMPSVPLSLP
                     SELLNQRPDIAAAAYALAASDEQLKSSRAQFLPTLQLTASDGVLLASALSIDPVHIWS
                     VGGSILAPLFQGGRLNAQFELATSARDEAAWQYRASVLKALQEVEDQLVAEKALREQQ
                     AALREQIQAVSSALQHANNRYHAGYAPYLEVLDAQRSLYQLQVQEIQLNSEVLLTQVA
                     LYQALGGGWVSAKKVN"
     gene            8262..8843
                     /locus_tag="PK856_00380"
     CDS             8262..8843
                     /locus_tag="PK856_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HRL00858.1"
                     /translation="MTLLAEILDHNKEFVDAGEYVQFFSDKFPNRGLAILSCMDARII
                     ELLNEALGLKNGDAKMIKNAGALVTHPWGSVMRSLLVAVFELRCKEIMVVAHYDCGMR
                     GLSSKHVLEKAEASGISATSIETLRNAAIDLDGWLTGFDDVGQSVRHSVEMIRNHPLM
                     PKSVPVHGLVIHPTTGKLDLMVNGYDNVSDDIK"
     gene            8840..9463
                     /gene="nadD"
                     /locus_tag="PK856_00385"
     CDS             8840..9463
                     /gene="nadD"
                     /locus_tag="PK856_00385"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HRL00859.1"
                     /translation="MKLGLFGGTFDPIHNGHLSMAAAFADELGLDQVVFIPAGDPYHK
                     SVATRTPAVKRLAMVEAAIASDVRFAASDIDMVREGVTYTVDTLQILRQYYPNAEMWW
                     LMGMDSLLSLHTWHRYQDIFKLANVAVAARNDEKIQQVAPEMRALVAQGLSQATSASK
                     GQLRLLQWQPEDISSTQIRNAVKSGLTIDGWVPKPVRDYISTHGLYR"
     gene            9486..9869
                     /gene="rsfS"
                     /locus_tag="PK856_00390"
     CDS             9486..9869
                     /gene="rsfS"
                     /locus_tag="PK856_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HRL00860.1"
                     /translation="MTDTVDLQKMVDVAVNALEDVKGKDIVVLDVSAKTSLFQRMIVA
                     SGDSNRQVKALVNNVAVDLKEQGFEVLSTEGTDSGEWALVDAGDVVVHVMLPAVRDYY
                     DIEALWGGEKPSFHSGHAKPWHAAD"
     assembly_gap    9964..9973
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9974..10885
                     /locus_tag="PK856_00395"
     CDS             <9974..10885
                     /locus_tag="PK856_00395"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRL00861.1"
                     /translation="KMASAVACLLVTNVIWAGNALLARHAGGSDMQPLTMNYLRWLIG
                     ALALLPFCGREIWQQRRLWGQPAVLWRMAVLAGCGMVVYNTLLYAAAHYTTAINISLL
                     NTCIPLATFITSGLILKQWPKPASWLGLGIAALGLLYLISSGQWQVLVSLSFNRGDLM
                     MLIGVLAWAVYLVLLMPWGKQMGVSAMAMLNLLMLTAIALMTPLFAWEVSQHGLPSMN
                     QSNVMTLAYIGLLVSVVSYLAWNYGIAKIGAAKASLSLYTMPVFAAILSYFILGEGLK
                     AYHWIGGAIIVVGLLLASVLGKEKTPA"
     gene            10917..11336
                     /locus_tag="PK856_00400"
     CDS             10917..11336
                     /locus_tag="PK856_00400"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRL00862.1"
                     /translation="MTVFEQTISVLPEHCDGLGHVNNVQYLHWAEDLAWAHSSALGLG
                     LARYQAMDCAMVAREHVLTYLAACFAGDALCLRTWFSHNDGLRLRREYRFVRLADGKT
                     VFQGHTDWVCVQLSTGRPKRMPVEFLAAYAVTDKEAT"
     gene            11498..11686
                     /locus_tag="PK856_00405"
     CDS             11498..11686
                     /locus_tag="PK856_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00863.1"
                     /translation="MGWSNGVLALIFMLALVGMLLGLMCVFQLQVNKVFGKLGLWLGS
                     LCAMCIGVLWWLAVNFDV"
     gene            complement(11878..12009)
                     /locus_tag="PK856_00410"
     CDS             complement(11878..12009)
                     /locus_tag="PK856_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00864.1"
                     /translation="MLAHIAQEKRVQGFDIIITRKAKNHPIPTAVHMAKVNTVTEAL"
     gene            12135..12605
                     /gene="rlmH"
                     /locus_tag="PK856_00415"
     CDS             12135..12605
                     /gene="rlmH"
                     /locus_tag="PK856_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HRL00865.1"
                     /translation="MNITVLAVGNKMPRWVSEATEEYAKRFGREINFSIKELKPEKRG
                     SGVNAEQGMLAEEGRILAAIPQGSFVVVLDERGQAPTSVQLSEHMGRWLQEGQDVCFI
                     IGGADGMTDKVKQQARLLMRLSSMTMPHGMVRVLLTEQLYRAVSILHNHPYHRE"
     gene            12714..14591
                     /gene="dxs"
                     /locus_tag="PK856_00420"
     CDS             12714..14591
                     /gene="dxs"
                     /locus_tag="PK856_00420"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009115842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HRL00866.1"
                     /translation="MSTPLLDNIEFPADLRQVAETDLPQVAEELREFLLQSVCATGGH
                     FASNLGAVELTVALHYVYNTPDDHLVWDVGHQSYPHKILTGRKGRMGTMRQQHGLAGF
                     PKRCESEYDAFGVGHSSTSIGAALGMQVADQLQGKPNRHVAIIGDGAMTAGQAFEALN
                     NAGDMPNTNLLVILNDNEMSISPNVGALPKYLAQSVMRDFKGLVKSAKNKSEKVFNKV
                     PGMMDVAKKVESKIKEAVDGSPLDPHVSLFEKFGFDYTGPIDGHDVALLVKTLKHLRS
                     QTGPQLLHIVTKKGQGYKLAENDPVGFHAIGKMDPETGPVKSTAVAKPTYTQIFGQWL
                     CDQAAADARLVGITPAMREGSGLVAFEKQYPDRYFDVGIAEQHAVTFAGGVACEGMKP
                     VVAIYSTFLQRAYDQLVHDVAIQNLPVMFAIDRAGIVGADGPTHAGAYDLSFMRCVPN
                     MVIAAPSDENECRMLLSTCYQLNQPSAVRYPRGSGTGVEADKDLQTVPVGKGVVRKQG
                     EKIAILAFGSMVAPSLQAASVLNATVADMRFVKPLDEALIQELAASHDYLITVEENAV
                     QGGAGSAVLEYMARQKICKPILNIGIADVVTEHGDPAAILADLGLNATGIEAAIREFT
                     A"
     gene            14876..15043
                     /locus_tag="PK856_00425"
     CDS             14876..15043
                     /locus_tag="PK856_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00867.1"
                     /translation="MESSLERNTETKMNSGEGLEQTSQVTQAWNQQDPLKIAQDLNRI
                     IQNMMHSGDEA"
     gene            15187..15687
                     /locus_tag="PK856_00430"
     CDS             15187..15687
                     /locus_tag="PK856_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HRL00868.1"
                     /translation="MKNFTYDELAVGQSGGLQKTVTAKDVLLFAAVSGDTNPAHLDPA
                     YAEQTMFKNVIVHGMYSAGLISAVLGTRFPGPGTIYLGQDLQFKRPVNIGDTLDVQLT
                     VASKRDDKKIVTLDCVVTNQEGKIVVTGTSTIIAPSLSVEHELLVLGEVAAPAAPNAQ
                     FEAFMK"
     gene            complement(15759..>16713)
                     /gene="phoR"
                     /locus_tag="PK856_00435"
     CDS             complement(15759..>16713)
                     /gene="phoR"
                     /locus_tag="PK856_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HRL00869.1"
                     /translation="LQIDDLGGILANLVRQPEFHHFMSEDSTQNEVKLHLPTRKGLPR
                     TVIIKRTPFDKHMQLLVSQDITPMEKLNQTRSDFIANVSHELRTPLTVINGFVETMQD
                     LPNLPADKREQFLALMKKDSDRMLTLLDDLLTLSRLENFDPSHKLQPIHLSALVEQLS
                     QEGMTLSAGKQTFDTNIEPNLWIQGVQLDLYNALSNLVFNAVRYNHDGGHIAIRLHSD
                     DGANVRFSVSDNGPGIAAEHIPRLTERFYRVDAGRSRASGGTGLGLAITKHALAEHGS
                     QLEVESTVGKGSEFSAEFALVGMPQAVTLSGADTTPEQAEP"
     assembly_gap    16714..16780
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16781..17108)
                     /locus_tag="PK856_00440"
     CDS             complement(<16781..17108)
                     /locus_tag="PK856_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3329 domain-containing protein"
                     /protein_id="HRL00870.1"
                     /translation="MNRFALQCLRTIAVFALIGVICGLVWGAGMGLMVFLGLLSLWLF
                     AHLWQVGQLIAWLERPKPSTIPTGLGVWQQVFNTLLMQAKSRKRRKQKITQALQRFYH
                     ASEVMPD"
     gene            complement(17109..>17344)
                     /locus_tag="PK856_00445"
     CDS             complement(17109..>17344)
                     /locus_tag="PK856_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRL00871.1"
                     /translation="FGPTEFKLLHFFMTHADRVYTRRQLLDLVWGDHVFVEERTVDVH
                     IRRLRRALAPAQLENLVQTVRGAGYRFSQKESA"
BASE COUNT         4178 a         4053 c         4797 g         4239 t
ORIGIN      
        1 gccatcggtt tattagacga aggcgcaacc gtgccattta ttgcgcgtta ccgcaaagag
       61 gtgacgggcg gtttggacga cacgcaattg cgcaacttgg ccgagcgctt gcaatacttg
      121 cgcgaattaa tcgatcgcaa agccaccatc ctcaaaagca tagatgagca aggtaagttg
      181 tcgcctgaat tggcggcgca aatcgaagcg atagacaaca aaaccgcact ggaagatgtg
      241 tatctgcctt acaaaccgaa acgccgcacc aaggcgcaaa ttgcgcgcga agcaggtttg
      301 gaacccttgg ccgaaacctt gtggcaaacg ccgaataccg accccgaaac cgaagcggcc
      361 aaatacctga atgccgagca caacattgcc gacagcaaag cggctttgga tggcgcgcgc
      421 gcgattttga tggagcaatt tgccgaagac gcgattttgc ttgggcaatt gcgtgagcgc
      481 ttgtggcaag tgggcgacat tcgcagcgag gtggtggcag gacaagagca gacaggcgaa
      541 aaattccaag actatttcaa acaccaagaa gccttgcgca gcctgccatc tcaccgcagc
      601 ttggctttgc tgcgcgggcg caatgagggc gtgttggcat tgaaaattgt ctatcaagcc
      661 gatgacacgc cgattaccga ggcgagcgag tttgagcaaa ccattgccgc gcattacggc
      721 attgccgacc aaaaacgcac ggctgacaaa tggctcaaag acacagtgcg cctcacttgg
      781 cgggcgaaat tgtttttgtc tttggagctg gacgcgttta accgcatcaa aaccgagggc
      841 gatgctgccg cgattacggt gtttgcgcgc aatttgaaag atttgctgtt agcagcgccc
      901 gcaggccgct tggtgacttt gggtctggac ccaggcatac gcacaggcgt gaaagtggcg
      961 gtggtagacg acacgggcaa gttgttggcc acagacacgg tgtatccatt ccaaccgcgc
     1021 aacgacaaac acggcgcgat ggcgagcttg gcgcgtttga ttaagcaaca cggcgtgcag
     1081 ttaatcgcca tcggcaacgg cactgccagc cgcgaaaccg accaattggc ggccgaagtg
     1141 atgaaggcga tgcctgaagc gggtgtgcac aaattggtgg tgtcggaggc gggcgcgtcg
     1201 gtgtattcgg cgtcggaatt ggcggcgaac gagttccctg atttggacgt gtctttgcgc
     1261 ggcgcggtgt cgattgcgcg acgcttgcaa gaccccttgg ccgagttggt gaaaatcgac
     1321 ccgaaatcca tcggtgtggg ccaataccaa cacgatgtca accaagtgca attggcgcac
     1381 agcttggatg cggtggtgga agactgtgtg aatgcggtgg gtgtggatgt gaacacagcc
     1441 tctgcgccgc tgctcgcacg cgtgtcgggt ttgaacgcga ccttggcgca caacatcgtg
     1501 gcctatcgcg atgaaaatgg cgcgtttaaa aaccgcaaac aactgaaaaa agtgccgcgt
     1561 ttgggcgaca aaacctttga gcaagcggca ggctttttgc gcatacaagg tggcactgag
     1621 cctttagacg cctcatcggt gcatcccgaa gcctatccca tcgtggcgaa aatgctcgcc
     1681 gacacaggca gcagcgccga caaattaatc ggcaatatgc cagtgttgaa aaccttacag
     1741 gccgctcgtt acatcgatgc acaatttggt ttgcccacgg tgatggacat tttgagcgaa
     1801 ttggaaaagc caggtcgcga cccgcgcggt gaattcaaaa ccgccacttt tgccgatggc
     1861 attgatgaca tcaagcattt ggtggtgggc agcatcatgg aaggcgtggt caccaatgtg
     1921 gcggcgtttg gtgcgtttgt tgatgtgggc gtgcaccaag acggcttggt acacatttcc
     1981 gccttgagca agcaatacgt gtccgacccc catacggtgg tgaaagcagg gcaagtggtg
     2041 aaagtgaagg tgttgagtgt ggacgtggcg cgcaaacgca tcgctttgac catgcgctta
     2101 gacgatgaca ttgagacagg cagccaacgc ggtaatgcca acaacagcgc aggcaatcac
     2161 caacgtggcg agcgccgtgg tggcaatgag cgcaaacgcg aagaagcacc tgtcaataat
     2221 gcgatggcgg atgcgtttgc caagcttaag cgttaatgga tgcagatatg tgcggctaaa
     2281 gcgccgcaca agtacaaaaa gcggcctgta agatttacag gccgctttta tattggtttt
     2341 tgtgttgata taaacggtat caaaatatgt tgttaagtga gttcaacaaa gggactgcac
     2401 agtcagtgca aggcggcacg gcatgaataa ggacaaggat ttgattgacg caggtgacat
     2461 gaaaccgaag cggcctgtaa accttacaag tcgcttgttt ggcatgagat gcgcgatatt
     2521 aaatggcttg ggaagctaag tcacatgaat gggtagatag cttgcgatac ctgtgcacgc
     2581 attggtgtat gagtcgtgag caaacttaaa agcggcctgt aaacatttac aggccgcttt
     2641 aagtttgttg tgagcgttaa atcgtaaaca gcaaagtcat caccaaacgg cgtacgtcgc
     2701 ggctttcgcg cacgtctacc atcaacacag ggatttcatc gcgaaactca tcggccactt
     2761 cttgaaagtc ttctaaaggg tgattgggat tgcttttgtc gactcggttg atgcctatga
     2821 ccaaaggaat gttttcatcc actatcaggc ctttgaacgc attgaggtaa aagcgcaaat
     2881 ctgcggcagg gtcggttgcg gcggcattga ttaaaatcgc caagcccatg ctgcctttac
     2941 tcaaaatctc ccacatgaaa tcgaaacgct gctggccagg agtgccatac atgtgcactt
     3001 tggtttcttc atccaacacc accgagccgt aatccatggc caccgtggtt tggtctttca
     3061 tattggccac atcgtctgag gggcgcacct cagtcaaaat cggggcatcg tcgctaaaag
     3121 ctttaatcaa cgtggtttta cccgccccaa ctgggccagt aaaaatgatt ttgtgttcaa
     3181 acatgctgtc tccgccctga ttcagggagt ttctaatagg ctgtggcgcc aatttggccc
     3241 ttgttgtggc tgccatgccg agtcataaat ggtgtgttgc gggttggtaa aacatttgat
     3301 ttggatttta tcgtaagttt tgtggccagg gatttgtttc aagccagtat ttaatcactt
     3361 gggcagtttt gtgttgtctt ttttcactga aaacccgatt gtcatcattc taacagttat
     3421 tgttacccaa gatgggtact ctaaattgac tgattttcgg ttttgggaat agcgattttg
     3481 taccgctgcc attttcaccg cagcatgaca gtttggtgtt gctaaagtat gaaaaacaga
     3541 atgggtggtg gtgtgttgga gcagaaaaac ggcgcttgca tttgaaaatc aatattcatg
     3601 agtacacggt atttatggga gtgactggct tgaaaacacc gttttgtaag gcttataggc
     3661 cgtattgaca aggattaggc gctaaaacca aaaaagagta gaatcaattc gcttgttttt
     3721 gactcctgct gtgggcttgt tgcgacacgt aagtgagccg agttaggtgg catggggcag
     3781 tgacaagtgt tgttttccgt accgagccat gcgtgggcaa ttaagtgttg attgcggcat
     3841 ggctttcagc ccatagcgca gaccatgaaa atcaaaaaac tgtggcaaac gccgcctgtg
     3901 acatacacgt ttaaaagcca tgaacaaccg ttcatgatag gttcccctgg tgtgcccgat
     3961 catcctaccc gccgcaaggt tttttacacc ttggtcggta tcgtgttggc cttaacaggc
     4021 ggcatgcaaa acggtttact cattgcgggc ttgccacaat tacaaggcgc tttggccttg
     4081 acccctaccg aagggggatg gattttggtg gcttacaaca tggtcaatgc ctgcatgagc
     4141 atgttgttgt tcaaatgtcg gcaacaattt ggtttggcaa gattcatccg cgccatcatc
     4201 atggctttgg tgttgtgcaa tctgatacaa gtcgcgaccc acagctacca cagtgaattg
     4261 gtggcgcgtg gcatcagtgg cttggctgcc agtggcatga ataccttggc cttgttttat
     4321 ttcatccaag gcatgccaac caagtggcgt ttaaaaggca tcgcctgttc catcagtgtg
     4381 tcgcaagtgg ccttgccttt tgcccgcgcc attgccccag ctttaatgaa cgatggcaac
     4441 attcaggcag tattttggct gcaattgggc ctgtctttga atgtgttggt gttggtgtct
     4501 ttgttgccgc tgccacatgg ctacacccaa aaagccatca gcaaaaccga tttgttttcc
     4561 ctgctgtgtt tcgcttttgg tgtggccgcg ctgtgtgctt tttttgtaca aggtcgcatc
     4621 atctggtggg ataaaatcgt attggggcaa ttgctgttgg tggctttggt gggcttgggc
     4681 atcaccttgt tgatagagtc caatcgccaa gaccccatga tcaaactcga ttggttgcgc
     4741 agcaaagaat tgctgtggtt gggtttgacc gctgcttttt tgcgcatggt gatgtcggaa
     4801 caaaatgttg gctcagcggg tttgctcacg gctttgggca tgggtaacac gcaaatggtg
     4861 acgtattatt gggtgttaac cgcaggggcg ttaacaggtt tggcagcgac attgtggttg
     4921 atggaaccgc aaaacctgaa gcccccagtg atgttggctt ttttctgcat tgcggtgggg
     4981 gcatttttgg atgtgggcgc caacagttta accagaccca gccaaaccta tgtcagccaa
     5041 ttcctcattg ccatggccag cgtgtgcttc atggcaccgt tgatgataga aggcatgttg
     5101 cgagcattct ctaaaagtcc cgctcacatc attaccttta ttgccgtgtt tggtttgtcg
     5161 caaaccatag gtggcttgtt cggttctgcg gccttgtcca cttttctcac cttgcgcacc
     5221 aaagagcatt taacccgctt tggcgaacaa ctgtccatga ccgaccctac tgtggccgct
     5281 cgcgtacaag cctatgcggc acaataccaa ggggtgatag gtgatggcgc gttgcgccaa
     5341 ggccaagggg tggcgggagt ggtggctcag gcgcagcaac aggcaacggt attggcgtat
     5401 aacgactcgt tttggttgtt tggattggtc gcatcttgtg gcttcttgtt cgtcagtgcc
     5461 gaatgggctt attacaaata ccacggtacc agccacatgg cccccgtgtt ggccgaattg
     5521 gccaaaatga aagccaagaa ataattttgg gagcagcaaa tggcagaatc cacgaaacct
     5581 gaagaacagc ggcctgtaaa cgcagaaacg ccgtctgaaa ccaaacaaga agcagaaacc
     5641 cctgcagcca atgcacaagc ggctgagcct gcagcacatg acaccaaaac caccgtcacc
     5701 gtggccgata attggcatcc acccaaaaaa acgcacctga aaaatgccgt gctcattgcc
     5761 gttggcttgg tagccattgc cttggtgctg tacgcatgga agctgccacc gtttcaaagt
     5821 gccgtggtca gcaccgacaa tgcctatgtg cgcgggcaaa ccaccgtcat cagcccacaa
     5881 gtcacaggct atgtgacccg cgtgtatgtg caagactttg aagacgttgc cgcaggcacg
     5941 cctttggtgc aaatcgaccc gcgcatttac cagcaaaaag tggagcaagc acacgcgcaa
     6001 gtgatggctg ccgaatccaa tttgcacaac aaccaacaaa gcctcacttc cagtcaatct
     6061 actgtcttgg cgcgcaatgc cagcatcgcc aatgctgaag cgatgttgaa aaaagcccaa
     6121 gccgacatgg cgcgggtaaa ggaattggtg ggcttgggtt ctttgtcgcc gcgcgaaggc
     6181 gaccaagccc gtgccgcttt gcaacaagca caagcggctg tggctgaagc ccgcgcccaa
     6241 aaagccgtcg ctgaacaagg ggtgaagtct acccatgtcg gtaaacaagg tttacaggcc
     6301 gctgtggcac aggccgaggc gcaagcacag ttggcacaga tagacttgga caacaccacc
     6361 atcaaagccc cacaaaacgg gcgtttgagt gaaattggcg tgaagcctgg ccaattggtg
     6421 aatgcgggca cgcagttgat gtatttggtg cccagccatg tgtgggtggt ggcgaatttc
     6481 aaagaaaccc aaaccgccaa tattcgcgtg ggccaaaatg ccaaagtgcg tgttgatgcc
     6541 ttgggtgggg ccgagttaac aggtaaggta gagcgcatcg cgcctgcggc gggcagtgag
     6601 ttcagcgtga tacgccccga caatgccacg ggcaattttg tcaaagtggc gcagcgcatt
     6661 gccgtgcgca ttcaaatcaa tcccaaccaa gagttggcca aacgcttgtc gccaggcatg
     6721 tcggtggaag cgagtgtgga cacagggagc aaatcatgag gcaggtgtgg tttttgagca
     6781 tggtgttggg tttgagtgct tgcgtgccga aaataccgcc acagcccagt gatgcggcct
     6841 taaatccacc tgagcaatgg ttacaggccg ttgctataaa tcacatgggt ttgggcgtga
     6901 ctttggcaga gccgcaaaca tggtgggcgc aattgggcga tgcgcaattg aatcaatacg
     6961 tcgctcaggc tttgcgttac aacagtgatg tcaacatcgc cgccacccgc gtacaggccg
     7021 ctatggcgca agaagcggtg aatcgcagcg tgttgtttcc caatttgtct gtgggcggcg
     7081 caggcagcaa tggtcgcagt gtgaatgcgt ttggtgtgcc cagccagtcg gcagtggctc
     7141 agcctgtgtt tcaagccagt tatgaagtgg acgtgtttgg taaaaaccgc cacgcttggc
     7201 gcgccaaaaa atggcaaacc gcagcggctg aggcacaaca agccgccacg caagtgagcg
     7261 tcagcacggc ggtggcgcgt ttgtatgtgc agctcaccgc cttacaagcg caaaagcaat
     7321 tgttgcaaaa cacactgcgt tcacgtgaac aagctttaaa aattgcccaa gacaaggcga
     7381 gggtcggtta cagctcgcaa ttggaattga accaagcgca agcagaatac gcagccacca
     7441 agcagcaaat tcccatcaac caaaaagcca tttctcaaag ccaacacgca ctcagcactt
     7501 taatcggcag tgtgccacaa gccttgcccg cggcggacag cttaaaaagc ctgcacatgc
     7561 catctgtgcc cttgagtttg ccctctgaat tgttgaacca gcggcctgac atcgcggcgg
     7621 cggcgtatgc attggcggca tccgatgagc aattaaaatc cagtcgggca cagtttttac
     7681 ctaccttgca attgacggca agcgatggtg tgttgttggc atcggccttg agcatagacc
     7741 cagtacacat ttggtctgtg ggcggcagca tactcgcacc tttgttccaa ggcgggcgtt
     7801 tgaatgccca atttgagctg gccacttcgg ctcgcgatga ggctgcatgg caataccgcg
     7861 ccagtgtgct caaagcctta caagaggtgg aagaccagtt ggtggcggag aaagcattgc
     7921 gtgaacaaca ggccgcactg cgggagcaaa ttcaagcggt cagctcggct ttgcaacacg
     7981 ccaacaaccg ctatcacgca ggttacgccc cttatttgga agtcttggat gcgcaaagaa
     8041 gcttatacca attacaagtg caggaaattc aattgaacag cgaggtattg ttgacccaag
     8101 tggcgttgta tcaggctttg ggtggcggtt gggtcagtgc caaaaaggtt aactagagtg
     8161 aaatcctcag ttttagagtc ttacccagtg aacgcttaca ggccgcatgt ggtagcataa
     8221 ccgcctataa aaataaccat ccaatcagta gagtaagaaa aatgacacta ttagcagaaa
     8281 ttttggacca caataaagag ttcgtcgatg cgggcgaata tgtgcagttt ttcagtgata
     8341 aattcccgaa tcgcggcttg gccatcttgt cttgcatgga tgcacgcatc attgaattgt
     8401 tgaatgaagc cttgggtttg aaaaatggcg atgccaaaat gattaaaaac gcgggcgcac
     8461 tggtaaccca tccttggggt tcggtgatgc gctcattgtt ggtggcggtg tttgaattgc
     8521 gttgtaagga aatcatggta gtggcgcatt acgattgcgg catgcgcggc ttgagttcca
     8581 aacatgtgtt ggaaaaggcc gaagcatcgg gcatttctgc caccagcata gaaaccttgc
     8641 gcaatgctgc gattgatttg gacggttggt tgacaggttt tgatgacgtg ggccaaagtg
     8701 tgcgccactc tgtggaaatg atacgcaacc atcctttaat gcccaagtct gtgcctgtgc
     8761 atggtttggt gatacacccg accacgggca aattggattt gatggtcaat ggttacgaca
     8821 acgtttcgga cgacattaaa tgaaattagg cttgtttggc ggtacgtttg atcccataca
     8881 caacggtcac ctgagcatgg cagcggcgtt tgccgatgaa ttggggctag accaagtggt
     8941 gtttatcccc gcaggggatc cataccacaa aagcgttgcc acacgcacgc ctgctgtcaa
     9001 acgcttggcc atggtggaag cggcgattgc gagcgacgtg cgttttgctg ccagcgacat
     9061 cgacatggtg cgcgaaggtg taacctatac cgtggatacg ctgcaaattt tgcgccagta
     9121 ttatcccaat gctgaaatgt ggtggttgat gggcatggat tcgttgttgt ccctacatac
     9181 ttggcaccgc taccaagaca tattcaagct ggccaatgtg gctgtagcgg cgcgaaatga
     9241 tgaaaaaatc caacaagttg cccctgaaat gcgggctttg gtggctcaag gcttaagtca
     9301 agcaacaagt gcaagcaaag gacagctgcg tttgttacaa tggcagcctg aggacatcag
     9361 ttccacccag atacgcaatg ccgtcaaatc gggtttaacc attgacggct gggtccctaa
     9421 gccagtgcgc gactacatca gcacccatgg tttataccgt taaccgcttt taagagtttt
     9481 agaacatgac agatacagta gatttacaaa aaatggtcga tgtggccgtg aatgctttgg
     9541 aagatgtgaa aggcaaagac attgtggtgt tggatgtcag cgccaaaact tccttgtttc
     9601 aacgcatgat agtggccagt ggcgacagca atcgccaagt gaaggccttg gtgaacaacg
     9661 tggccgtgga tttgaaagaa caaggttttg aggtgttgag caccgaaggc accgacagtg
     9721 gcgagtgggc tttggttgac gctggcgatg tggtggtgca cgtgatgttg cctgccgtgc
     9781 gcgactatta cgacatcgag gctttgtggg gcggcgaaaa accttcgttc cattcgggtc
     9841 atgccaaacc ttggcatgct gccgattaag ttgcaacctg attcaagcaa tcacacaagc
     9901 ggcctgtaag ctttacaggc cgcttgtttt ttattcagca tttggaatgg aacggcttca
     9961 catnnnnnnn nnnaaaatgg ccagtgcggt ggcgtgtttg ctcgtcacca atgtgatttg
    10021 ggcgggcaat gccttgcttg cgcgccatgc gggtggcagc gacatgcaac ccttgacgat
    10081 gaattacctg cgttggttga ttggcgcttt ggccttgttg ccgttctgcg ggcgtgagat
    10141 ttggcaacag aggcgattgt gggggcagcc tgcggtattg tggcgcatgg ccgtgctcgc
    10201 gggttgcggc atggtggtgt acaacacctt gctgtatgcc gccgcgcatt acaccactgc
    10261 catcaacatt tctctgctca atacctgcat ccctttggcg acattcatca cttcaggctt
    10321 gatattgaaa caatggccca aacccgcttc gtggttgggc ttgggcatcg cggcgctggg
    10381 cttgctgtat ttgatcagtt cgggacagtg gcaggtgttg gtgtctttga gctttaaccg
    10441 cggcgatttg atgatgctca tcggtgtgtt ggcgtgggca gtgtatttgg tcttgctcat
    10501 gccttggggc aagcaaatgg gtgtgtcggc gatggcgatg ctgaatttgc tgatgctcac
    10561 agcaatagcc ttgatgacgc ctttattcgc ttgggaagtg agccagcatg gtttgcccag
    10621 catgaaccaa agcaatgtga tgactttggc ttacatcggt ttgctggtgt cggtggtgtc
    10681 gtatttggct tggaattatg gcatcgccaa aatcggcgct gccaaagcct ctttgtcttt
    10741 gtacaccatg cctgtgtttg ccgcgatttt gagttatttc atcttgggcg aaggcttgaa
    10801 agcctaccat tggataggtg gcgccatcat tgtggtgggt ttgttgttgg ccagtgtgct
    10861 cggcaaagaa aaaacacccg cttgaatgcg gcctgtaaac ccttaggaca gcctcaatga
    10921 cggtatttga gcaaacgatt tcggtattgc ctgagcattg tgatggcttg gggcatgtca
    10981 acaatgtgca gtatttgcat tgggcagaag acttggcttg ggcgcattca agtgccttgg
    11041 gtttgggttt ggcgcgctac caagccatgg attgcgccat ggtggcgcgt gagcatgtat
    11101 taacctatct tgcggcctgt tttgcaggcg atgcattgtg tttgcgaact tggttcagtc
    11161 acaacgatgg cctacgcttg cggcgtgaat accgttttgt gcgcctagcc gatggcaaaa
    11221 cggtgtttca aggccacacc gattgggtat gcgtgcaact gtctacaggc cgccccaaac
    11281 gcatgcctgt ggagtttctg gctgcttatg cggtgactga caaagaggca acatgaagcc
    11341 tatacctgaa aacgacatgt accgcagcac agtgttgaat cacagcgttg tcacttccca
    11401 cccacacagc aaattgggca tggtttcttg tgggtgttcg gtactggcat agctgttgtt
    11461 ggtggtgttg gcattgaagc ctgagtgggt attcatattg ggctggtcaa atggggtgct
    11521 tgccttgatt tttatgttgg ccttagtggg aatgttgttg ggtttgatgt gtgtgtttca
    11581 actgcaggtg aataaagtgt ttggtaagct gggtttgtgg ttgggcagct tgtgtgcgat
    11641 gtgtattggt gtgttgtggt ggttggctgt gaattttgat gtataagcgg cctgtaaata
    11701 caaaagaccg acagggtgaa agggatgccg cgtgaaagac aacaatccat atcgcagtag
    11761 tgttttggca gagccccaac aaacatctgt gaacagtgac aagtctttaa gccgttggtc
    11821 atggatttgc gcattgttgg cgtgcttgtt ttacctgtta cgtgcatacg accctgctta
    11881 caatgcctct gtcacggtgt tgaccttggc catgtggacg gctgttggga tggggtggtt
    11941 tttggctttg cgggtgatga tgatgtcaaa tccctgtaca cgtttttctt gggcaatgtg
    12001 tgctaacacc agcttgctgc tgttcttttt gatggcttaa tggcttacag gccgcttgtt
    12061 taagcctttg cttcgttttc acctaaacag cacaatccgc tataatcaag cattgtggca
    12121 aacaaacaga atcaatgaac atcacagtct tggcggtagg caacaaaatg ccgcgttggg
    12181 tcagcgaggc gaccgaggag tacgccaaac gctttggccg cgaaatcaat ttcagcatca
    12241 aagaactcaa gcccgaaaag cgcggctctg gggtcaacgc cgaacaaggc atgttggccg
    12301 aagaagggcg cattttggcc gcgattccac aaggcagttt tgtggtggtg ttggatgaac
    12361 ggggtcaagc gcccacttcg gtgcaattgt ctgaacacat ggggcgctgg ctgcaagaag
    12421 gccaagacgt gtgttttatc atcggcggcg ccgacggcat gaccgacaaa gtcaaacagc
    12481 aagcgcgttt gctgatgcgc ttgtccagca tgaccatgcc acacggtatg gtacgtgttt
    12541 tgctcactga acagttgtat cgcgccgtgt ccatcttgca caaccatccg tatcatcgcg
    12601 aataagccgt gaaaaacagg tgaatttgtg tgagcggtcg caatcagttc cgacaagtgt
    12661 ggctgaatcg gctaaaataa acccaagtct ttgcacctaa cgataccaaa aatatgagca
    12721 cacctctttt agacaacatt gaatttcccg ccgatttgcg ccaagtggct gaaaccgatt
    12781 tgccgcaagt ggctgaagaa ttgcgagaat ttttgttgca atcggtatgc gcaaccggtg
    12841 gccatttcgc cagcaatctg ggtgccgtgg aactgaccgt ggccttgcat tatgtgtaca
    12901 acacccctga cgaccatttg gtttgggatg tgggtcacca aagctatccg cataaaatct
    12961 taacaggccg caagggccgt atgggcacca tgcgtcagca acatggtttg gcagggtttc
    13021 ccaagcgctg cgaatctgaa tacgatgcgt ttggcgtggg ccattcatcc acttccatag
    13081 gcgcggcctt gggcatgcaa gtggccgatc aattgcaagg caagcccaat cgccatgtgg
    13141 ccattattgg cgatggcgcg atgacggcag gtcaggcatt tgaagccttg aacaatgcag
    13201 gcgacatgcc caataccaat ttgttggtga ttttaaacga caatgaaatg tcgatttcac
    13261 ccaatgtcgg cgctttgccc aaatacttgg cacaatcggt gatgcgcgat ttcaaaggct
    13321 tggtaaagtc ggcgaaaaac aaatctgaaa aagtgtttaa caaagtgcct ggcatgatgg
    13381 atgtggccaa aaaagttgaa agcaaaatca aagaagccgt ggatggttcg ccattagacc
    13441 cacacgtttc tttgtttgaa aaatttggct ttgattacac tggcccgatt gatggtcatg
    13501 atgtggcttt gttggtgaaa accttgaagc atttgcgcag ccaaactggc ccgcaattgc
    13561 tgcacatcgt caccaaaaaa ggtcagggct acaaattggc tgaaaacgat cctgtcggtt
    13621 ttcatgccat cggtaaaatg gatcctgaga ctgggcctgt caaatccact gcggttgcca
    13681 aacccactta cacgcaaatt tttggccagt ggttgtgtga ccaagcggct gccgatgcgc
    13741 gtttggtggg cattacccct gccatgcgtg agggctctgg cttggtggcc tttgaaaaac
    13801 agtatcctga ccgttatttt gatgtgggca ttgccgaaca acacgcggtg acgtttgcag
    13861 gcggggtggc ttgtgaaggc atgaagcctg tggtggcgat ttattccact ttcttacagc
    13921 gggcttacga ccaattggtg catgatgtgg ccattcaaaa tttgcccgta atgtttgcca
    13981 tagaccgcgc gggtattgtg ggggccgatg gcccgaccca tgcaggtgct tatgatttga
    14041 gctttatgcg ctgtgtgcct aacatggtga ttgccgcacc ttcagatgaa aacgaatgcc
    14101 gcatgctgtt gtccacatgt taccaactga atcagcccag cgcggtgcgc tatccacgcg
    14161 gcagcggtac gggtgtggaa gcagacaaag acttacaaac cgtgcctgtg ggcaaaggag
    14221 tggtgcgtaa gcaaggtgag aaaatcgcca ttttggcttt tggcagcatg gtggctccaa
    14281 gtttacaggc cgcatctgtg ttgaatgcca ccgtagcaga catgcgtttt gtcaaacctt
    14341 tggatgaagc cttgattcaa gaattggctg ccagccatga ttacttgatt acggtggaag
    14401 aaaacgccgt gcaaggcgga gcaggcagtg cggtgttgga atacatggcg cgtcaaaaaa
    14461 tctgcaagcc gattttgaac atcggcattg ccgatgtggt gaccgaacac ggagaccctg
    14521 cggcgatact ggcggatttg ggcttgaatg caacgggtat tgaagccgcc atacgtgaat
    14581 tcacagcgta atcaagtgat aatatacaga cagcaagcca cattgggctt gctgtttttc
    14641 tttgtattgg gtcttgatgg ataagctatt attggaaatt gtggcattgt tgtgggcaat
    14701 tatttaacca tttgtcattc gttattgctt gattttaaat atcaaaacac catttaaaag
    14761 ccccgattat ttgacataaa ccaaaactgc tgtaaattca tggtgttact ctagagaatc
    14821 tcatttatga tgagccttca attcattagc atttaatcac ggtttggagc agagcatgga
    14881 atcatcattg gaacgcaaca cagaaaccaa aatgaattct ggagaaggct tggagcaaac
    14941 ctctcaagtg actcaagctt ggaatcagca agacccgctg aaaattgccc aagacttgaa
    15001 ccgcatcatt caaaacatga tgcacagtgg cgacgaagcc taaattcagt caaaacacca
    15061 cctgagggtg gtgtttttgt atgggtcgca atatccgttt atcagagact ctgttaggat
    15121 agggcgcact tatgcacccg tcgcatgtgc gcggagccta tgggtaaacg aatagatgga
    15181 gaagcaatga agaattttac ttacgatgaa ttggctgtgg gccaaagcgg tggtttgcaa
    15241 aaaaccgtaa cggccaaaga tgtgttgttg ttcgctgcgg tcagtggcga caccaatcct
    15301 gctcaccttg accctgcata tgccgaacaa accatgttca aaaatgtgat tgtgcatggc
    15361 atgtacagcg cgggcttgat ttctgcagtg ttgggtaccc gttttccagg accaggtacc
    15421 atctacttgg gacaagattt gcaattcaag cggcctgtaa acataggcga caccttggat
    15481 gtgcaattga ctgtggcaag caagcgcgac gacaaaaaaa tcgtgacctt ggactgcgtg
    15541 gtgacgaatc aagaaggcaa gattgtggtg acgggaacgt ctaccattat tgcccccagt
    15601 ttgtcggttg agcatgaatt gttggtattg ggtgaagttg ctgccccagc cgcaccgaat
    15661 gcccaatttg aagcctttat gaaataagcg ttactgcaaa aaagcggcct gtaaaccata
    15721 tctattggtt tacaggccgc tttttgatgc tgtcttgttt atggttcggc ttgttctggc
    15781 gtagtatcag cgcctgacaa ggtcacggct tgtggcatgc caaccaatgc aaactcagca
    15841 gaaaactcac tgcctttgcc cacagtggat tccacttcca actggctgcc gtgttcggcc
    15901 aaagcgtgtt tggtaatcgc cagacccaag cccgtgccac cgctggcacg cgagcgccct
    15961 gcgtccacac ggtaaaagcg ctcggtgagg cggggaatgt gctcggcagc gatgcccggg
    16021 ccattgtcgc tcacactgaa gcgtacattc gcaccatcat cgctgtgcag acgtatggcg
    16081 atgtggccac cgtcatggtt gtagcgcaca gcgttgaaca ccaaattgct caaggcattg
    16141 tacaaatcca attgcacccc ttgtatccat aaattcggct cgatgttggt atcgaaggtt
    16201 tgtttgcctg ccgacagtgt cataccttct tggctcaatt gttccaccaa agccgacaaa
    16261 tggatgggtt gtaatttgtg cgacgggtca aaattttcca aacgcgataa agtgagcaaa
    16321 tcgtcgagca aggtcagcat gcggtcgctg tcttttttca tcaaagccaa gaattgctcg
    16381 cgcttgtccg caggcaagtt gggcaagtct tgcatggttt caacaaagcc attaatcacg
    16441 gttaagggcg tgcgcaattc gtgcgacaca ttggcaatga agtctgagcg ggtttgattg
    16501 agcttttcca tgggcgtgat gtcttggcta accagcaatt gcatgtgttt gtcgaatggc
    16561 gtgcgtttga tgatgacggt gcgaggcagc ccttttcgtg tgggcaggtg cagcttcacc
    16621 tcgttttggg tgctgtcttc tgacataaag tggtgaaatt cgggttggcg caccaaattt
    16681 gccaaaatgc cgcccaaatc atcgatttgc agcnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn catcaggcat cacttccgag
    16801 gcatggtaaa accgctgtaa ggcttgggtg attttttgct tacgccgttt gcggcttttg
    16861 gcctgcatta gcaaggtgtt gaacacttgc tgccacacgc ccaagccagt gggaatggtt
    16921 gagggcttag ggcgctctaa ccacgcaatc aattgaccga cttgccaaag atgggcaaac
    16981 aaccacagcg acaacaagcc taaaaagacc atcaagccca tgcccgcacc ccataccaaa
    17041 ccacaaatca ccccaatgag ggcaaacacc gcaatggtac gcaaacattg cagggcaaat
    17101 cggttcatct aagcactttc tttttgcgag aagcggtagc ccgcaccgcg cacggtttgc
    17161 accaagtttt ccaattgagc gggggccaag gcgcggcgca agcggcggat gtgcacgtct
    17221 acagtgcgtt cctcgacaaa cacatggtcg ccccacacca agtccaacaa ttggcggcgg
    17281 gtatacacac ggtcggcatg ggtcatgaag aaatgcagca gcttgaattc ggtggggcca
    17341 aaat
//
LOCUS       DAOMVK010000005        15940 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_746_length_15940_cov_3.579918, whole genome shotgun sequence.
ACCESSION   DAOMVK010000005 DAOMVK010000000
VERSION     DAOMVK010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 15940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15940
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_746_length_15940_cov_3.579918"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            291..1019
                     /locus_tag="PK856_00450"
     CDS             291..1019
                     /locus_tag="PK856_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HRL00872.1"
                     /translation="MLDLRQYAALSFDVYGTLIDWEAGIAAVLGTWAQRHGADLSDEA
                     LLLAYADNEAAVERECPQLVYPEVLQAAFRRTAKDLQLPVSDADVQSLGRSVPDWPVF
                     ADTVEALGRLKQHYQLIILSNVHEEGVTGSIKHMHIAFDQVLTAEAIGSYKPNAHNFQ
                     VLLERVVDAGHTPTQLLHVAQSLFHDHVPAKAMGLDTVWINRRHNKPGWGATPNPNTE
                     VQPDLEFASLAAFADAVDAAFIKA"
     gene            complement(1090..2040)
                     /locus_tag="PK856_00455"
     CDS             complement(1090..2040)
                     /locus_tag="PK856_00455"
                     /inference="COORDINATES: protein motif:HMM:NF022839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcNAc-transferase family protein"
                     /protein_id="HRL00873.1"
                     /translation="MMKSLSEATIFVAIAAYRDPQLPLTLASLFAQAQYPERVYVGVF
                     NQIHLQEDQDCVAPERANVLQHVLDYNEAQGACWARSYVWKTLLAEQDFALQIDSHSR
                     FAPHWDTRLLQMYAEIGDARAVITHYPMGFDTETGEQDPLMYTYFNVQSFLPTQLPEV
                     TSGAVDLQAAPKRCAQTAFVAAGSLFGRAEMFKQVPYDPYIYFHGEEITYATRLWTHG
                     YNLYLPNRPYMWHDYKNLNQRPLHWQDHKQWALKDELAQQRCRHLLRIKQADDVRALK
                     SLEQYGMGFERSLDEYQVFSGIHFRRQTLEDKAKSGKQYG"
     gene            2242..2664
                     /locus_tag="PK856_00460"
     CDS             2242..2664
                     /locus_tag="PK856_00460"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRL00874.1"
                     /translation="MSLNQQQITALLLQHFPQIQDEISIDSLTEHEAIVRLRVNNRHL
                     RPGNTVSGPSMFLLADVAAYIAILAQVGARTQAVTTSAHIDFLRKPQAQADIIATTKV
                     LKIGKQLAVTDVYLYSENDSNMVARAAMTYSLPPVNQA"
     gene            complement(2655..3023)
                     /gene="speD"
                     /locus_tag="PK856_00465"
     CDS             complement(2655..3023)
                     /gene="speD"
                     /locus_tag="PK856_00465"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HRL00875.1"
                     /translation="MQAMGQHCLFDAYGIDCQWARDAKQIEALLTRVALAADATILGS
                     HFHTFGGEGGVTGVLLLAESHLSIHTWPENAYAAIDIFMCGHHRIEAALQLLRSELQA
                     DHTHTTVLSRGTLTSSVNHA"
     gene            complement(3033..3176)
                     /locus_tag="PK856_00470"
     CDS             complement(3033..3176)
                     /locus_tag="PK856_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00876.1"
                     /translation="MKGFFSWYAIALLVISTGLNYWMVIDSSRSSSGGSSIIRTGGGS
                     FHK"
     gene            complement(3186..4829)
                     /locus_tag="PK856_00475"
     CDS             complement(3186..4829)
                     /locus_tag="PK856_00475"
                     /inference="COORDINATES: protein motif:HMM:NF025158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HRL00877.1"
                     /translation="MASLFHAPCPSCGAEIEIHSATAVTEVCGYCHSMLILEGQALTE
                     SGRHSAVLRDFSPLQIGTSGSWKGAPFTLIGRLQVQYDGGSWNEWHALMADGSSAWLS
                     ESSDRFVFTRLETKAQIGIIPKYADMKIGRTFFAYGPKKYAAADVREVSRGRFAAEGE
                     LPYLVPEHETARVVDCRSADVFMTLDYTQDENKPEVFIGEGVTLNALRLQNTRDLQQI
                     KDSAGKLKGSAQQSKCPNCGGPVRWVTGVATHVVCEYCQSQIEFNEGQVKLVAANDMR
                     AAQDEALTIKIGSKARIMAIDWWVIGAMKQSEVRGDEASQAAFSYNAPKVLVPAGEPW
                     FEYLLYSPKEGFLWLTELSGNRWAIAKSLDVWPTLQQPLRPVDTNNRQVPELYDYGGQ
                     VQYATGAFYWQVGPKDTTYYVDFGREKQKLSTALMREEQSWSAITEIPAHAVAAWFKQ
                     SSISNKPMELSAADQLARQALRLEASHFNGNMADKLRTKAEQNGGGRAASPLIWLLIY
                     GVLNFPAMLMGLVGDGFFGVVIISFIVYWLLNLPFNNQE"
     gene            complement(4846..5913)
                     /locus_tag="PK856_00480"
     CDS             complement(4846..5913)
                     /locus_tag="PK856_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRL00878.1"
                     /translation="MVFKSFIKKQFIDVIQWPNPEPEVLMWRFPIQDEEIQNGASLTV
                     RDSQAAMFVDEGVTADVFQAGTFKLTTQNLPVLTNLKNWSKLFESPFKSDVYFFNLRQ
                     QLSRRWGTSQPVTVRDADFGAVALRAFGMYSFRIADPALFFKEVTGVTAEYRGEQLEE
                     QLRNVAVTQLATAFGTSGIPFLDMAANQVALSQKMTELLQPLFAAMGLLLESFNVESV
                     TLPEALQKRMDERMGMGIVGDLSRYTQYQTAQAIPIAAQNEGGVAGIGAGLAAGMGMG
                     NAMTAAMMGQQVAPQGQPVVQAAQTAAPVAAAAVAATAPAAGEDLAGKLTQLKTLLDQ
                     GLISQADYDTAKAEVIKKITG"
     gene            complement(5928..6326)
                     /locus_tag="PK856_00485"
     CDS             complement(5928..6326)
                     /locus_tag="PK856_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HRL00879.1"
                     /translation="MDIAVWQYLQYLKYFGVALVMLVVFAFAYVKITPARELELIKNG
                     NVACALSLSGAMIGFCITLVSSMLHSVGMLSFVIWGVAAAVIQILVYFAATHLIPEAN
                     AELQNNNVAVGVLFFGLSVSIGILNAASLS"
     gene            6511..8019
                     /locus_tag="PK856_00490"
     CDS             6511..8019
                     /locus_tag="PK856_00490"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HRL00880.1"
                     /translation="MNPALIVSVFIVASCGLAYELIMAALASYLLGDSILQFSSIIGL
                     YLFAMGIGAHLTRYIKDEDALMRFIEIEILVGVIGGISALALFVLFGFSAAPFRIILY
                     GLVLIVGTVVGMEIPLVMRVLNQHKADFKEIVSRVLTFDYLGALAVSLLFPLVLAPKL
                     GMARSALLFGILNVAVALITARIFKRDLPKYNRLRWRGGLVMLALLASFWYADNITYK
                     AEQNYFGDPIVYSSSSPYQRLVVTKWHNDTRLFINGNLQFSSADEARYHEALVLPVME
                     QLPEVKEVLILGGGDGLAAREVLKYPQVEHITLVDLDPEMTKLFRTSKELSGLNQQSL
                     NNSKVKIYNTDAAKWLETSRQQFDVIIIDLPDPSNFSLGKLYSVPMYRMVKRHLKTGG
                     KIVVQSTSPYFAPQAYWCIVTTLETAGLKTLPYHAYVPSFGEWGYVMASLQDDFKVPT
                     AYQVPTRFLDKTTTAQMFVFPKDMPRLNVEANHLNTQKLVEYFESDWRAVMR"
     gene            8019..9650
                     /locus_tag="PK856_00495"
     CDS             8019..9650
                     /locus_tag="PK856_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009119640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRL00881.1"
                     /translation="MMKLSRRRFLGHVLGAGLVSAAAYGTYRGFVPGYPKIHLDYPGM
                     DLGHAMRDALIKRPVNIPSTDVNVLIAGSGGAGLSAAWALSKQGMHDYVVLDGPERNG
                     NNRGEHNYGVTYPTGAHYLPLPAAESTHIQALLGDLDIYQNGRYDESAMVNAPSERLY
                     YHNKWQPEIIPDHDADSERFFRHMHTLSAQRGSDGKRLFVMPITLSSMDATWRALDQI
                     TFATWLQQQHYQSESLLWYCDYCCRDDYGQGIHQVSAWAGLHYFCARNSDGKDGHTVL
                     TWPQGLNELSERMRRYCKLQNHADVHTYRPLVHTPTALSASALNIEEHAQGVRVLCRD
                     GKGNLRYLNAQKVICAMPLYIAARVVNNIQQYGFEPQQHLPEYAPWLVSNFILKGFPT
                     EKSGAPLAWDNVAYQGQGLGFVLATHQEIWRSPPPYTSFTAYNALNHDSPQAIRHWLV
                     NSSPEAIYQHAAAEIEHMYGLQLRRHMIEARITVRGHAMAAPTPQYLNNSGLQALQNH
                     TGRLCFAHSDLSGYSVFEEACWWGQAAAEKILRPA"
     gene            9915..10376
                     /locus_tag="PK856_00500"
     CDS             9915..10376
                     /locus_tag="PK856_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00882.1"
                     /translation="MTKLSILSLCAVLALAACGGGEKAASDKPADSTATTSTAPAEPA
                     KAGGDPALDKEIDAGLAQVKGQLPMKLGDTMEMTSMERNGTDIVYTYTFLTDAVKKDN
                     FKVEDGKKAGAQQLCANAETKKYLDAGYSFNFNYKFKDGSNLEVPFKAGDC"
     gene            10463..10888
                     /locus_tag="PK856_00505"
     CDS             10463..10888
                     /locus_tag="PK856_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00883.1"
                     /translation="MSIKNGLAVCGCILALSACDYFGKGSNEAQNQSGPSRKLDTLID
                     AELAAMRLSLPQKMADEFDLTDTRRVGKDVTYTYQFHTQVNKASNFDVEAGKKVAIPE
                     LCDTKATREYLDAGYRFVFTYLFKDGSDVVVKIVAADCK"
     gene            complement(10938..11405)
                     /locus_tag="PK856_00510"
     CDS             complement(10938..11405)
                     /locus_tag="PK856_00510"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRL00884.1"
                     /translation="MKKIQACVWAIAALLLVACGESKPKPELVLQDLHGKKVSLQQLQ
                     GKPAVINMWATWCAPCRREMPVLQQAQQNHPHIQFVLLNQGEKAPVISAYLQKNQIQL
                     ANVWLDETMQSRTVLPYQGLPSTYFLNQQGEVVAHSLGELTPDQLQQYLQQIQ"
     gene            11553..11858
                     /locus_tag="PK856_00515"
     CDS             11553..11858
                     /locus_tag="PK856_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00885.1"
                     /translation="MKHVISLAVLLALSTVAMANNNPAQRCKRYAEANAFQSTVAELC
                     GGNTQSEFSGVMKGQACEETVGKEKLKTQGSTQSDELKAEYNRIGHTQFCSKYAGRK"
     gene            11926..12234
                     /locus_tag="PK856_00520"
     CDS             11926..12234
                     /locus_tag="PK856_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00886.1"
                     /translation="MKHLLGSLLAVAAFASSSFAYADAACSNLARDLYSAQGYQQRCG
                     YQLKQTPNLSTQFKTQKCDQSLSGADKNKMLNEVNNTLNQSFNASKSKSCDEGKSRFN
                     "
     gene            12299..12628
                     /locus_tag="PK856_00525"
     CDS             12299..12628
                     /locus_tag="PK856_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00887.1"
                     /translation="MFSRILLASALLALPLVSFAQTPPCMNLLTQSAQAGVAAKVCQK
                     QVNMEAIAQLHQQNQCAAFFAQDKVKNQINQVASQASHQAAQEAQQLGSQRYCQQAAV
                     NLGSLLK"
     gene            12680..13021
                     /locus_tag="PK856_00530"
     CDS             12680..13021
                     /locus_tag="PK856_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00888.1"
                     /translation="MWRIPTIIALGLGLGFSNQTWADNDRNTCSQLAIGIGQFEKVRT
                     VCDTGNIDATLWQSQLKSNQCSQVLGDDVLTQIINTAHNDAGATAETKGRTQYCEQDG
                     LQLLQQLSSPM"
     gene            complement(13081..13686)
                     /locus_tag="PK856_00535"
     CDS             complement(13081..13686)
                     /locus_tag="PK856_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRL00889.1"
                     /translation="MSVANFLESISRRRSIYNIGKNVSLSQEEIVNVIEEVVEQSPSA
                     FHAQSSRVVVLFGYEHQKLWNIVKDTLRPMIPAEQFAQTEGKIDGCFAAGVGTVLFFE
                     DQEVIKGQQAQFPLYADKFPEFSEQHAGMAQFMVWTALAELGLGATLQHYNPLIDNAV
                     AAAWNLPASWTLRAQMPFGSIEQAAEPKTFIARADRFKVFG"
     gene            13912..15690
                     /locus_tag="PK856_00540"
     CDS             13912..15690
                     /locus_tag="PK856_00540"
                     /inference="COORDINATES: protein motif:HMM:NF021432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 family protein"
                     /protein_id="HRL00890.1"
                     /translation="MAKFQSKHIKMAVGGILIATVAIGGWRFAQNRMGSFSQVDAIHI
                     DMAKPDVVIQSDSLNQLPSKLLTIPVIKDILTQEVVFYYQEAPAKQSLQGALQRLAFD
                     HNLRFEDRIIDKMLARKGDLYVWKGHTNKPDHWLFVGNSDAFSKFSEVLAKVALDDSQ
                     LIQVDTLKVDGNKIPLYALTYQGKTGLFAATSERVVFLSDAGMLMDLKSKPAKELQLE
                     AKVDTDKDGNEKVTPLPMSKAEKPEGKLISERADMVAKLLSKRPENQQAINQGLGLSK
                     AAQHPHSVYFSAAMLTLSYQDFMGGFQGSRFEFDGNTWQQAIALKPSLLPNHEWNGTD
                     LFAHMPHNAAFCANAPIEWGSLNTQAKKLPTATDALNLAQFSSPVLACWYAGSPAAAP
                     LFVTKLPKTEQREAQLAALQQVFSDVIGAKEYTHKQRFEVSRKETQGVVVNERIVSAR
                     YGSHDIDDLPADTAKELSATSYFPVRYAVSGDYVWFSPNGDLVEQAVAVSQQQAPALA
                     EQIQQQNSALLWVNGAELAKWLQQRTSAVIAEDSDMRTVLSQRLTPKLRLISSSGSWQ
                     VQADTKGLNPSSNNTQWVNVTWQHLP"
     gene            15672..>15940
                     /locus_tag="PK856_00545"
     CDS             15672..>15940
                     /locus_tag="PK856_00545"
                     /inference="COORDINATES: protein motif:HMM:NF018387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HRL00891.1"
                     /translation="MATPPLSRRDCLAYLAALSLAAPAQALSGLAEAKTSARLDAEQS
                     RLFRLWFAAIVRQQVAQGPSVRWQHRDCAGLVRFAVFETLRSHDAA"
BASE COUNT         4188 a         4255 c         3694 g         3803 t
ORIGIN      
        1 gcattgaact ggttgcaagt gcgcttgtta caagacttga aacaagcgcc acaagacacg
       61 caattgttgg cgatggtaca ttccaccatc aacggcatcg cccaaggttt gcgcaacacg
      121 ggttaacccc tccaaagcgg cctgtaaact caaaacaccc agtatcaact gggtgttttt
      181 tcatgtgtct ttgattttca ccttacatga aagccataca tccgcaggct aggcatagtc
      241 caagctttcg catacagtaa ggtgtctttc aaacacacaa ggagagggtc atgttggatt
      301 tacgacagta tgcggctttg agttttgatg tgtatggcac tttaatcgat tgggaagcag
      361 gcattgcggc ggtattgggt acttgggcgc agcgccacgg tgccgattta agcgatgagg
      421 ctttgctgct cgcctatgcc gacaatgaag cagctgtaga gcgcgagtgc ccacaactgg
      481 tctatcccga agtgttacag gccgcttttc gccgcaccgc caaagattta caactgcctg
      541 tgagcgatgc cgatgtacaa tctttgggcc gctccgtgcc cgattggccc gtgtttgccg
      601 acaccgttga ggccttgggc cgtttgaagc aacattacca attgattatc ttgtccaatg
      661 tgcatgaaga aggcgtgacg ggcagcatca aacacatgca catcgcgttt gaccaagtct
      721 taaccgctga agccataggc tcgtacaaac ccaacgcgca caattttcaa gtgttgttgg
      781 aacgtgtggt cgatgcaggc cacacgccca cgcaattgtt gcatgtggcc caatccttat
      841 ttcacgacca tgtgcctgcc aaagcaatgg gcttggacac ggtatggatt aaccgccgtc
      901 acaataagcc tggctggggt gccacgccta atccgaatac cgaggtacag cccgatttag
      961 aattcgccag cttggccgcg tttgcagatg ctgtggatgc ggcttttatc aaggcttaaa
     1021 cctcaatcca agcacaaaca agcggcctgt aaatgtacag gccgcttgtt ttgaatcttg
     1081 ctgctttcat taaccatatt gcttaccgct tttggctttg tcttctaagg tttgccgccg
     1141 aaaatgtatg ccagaaaaca cttggtattc atccaagctg cgctcaaatc ccattccgta
     1201 ttgctccaag cttttcaagg cacgcacatc atcggcttgt ttgatgcgca gcaaatggcg
     1261 gcagcgttgt tgggccaact catctttcaa tgcccactgt ttgtggtctt gccaatgcaa
     1321 aggcctttgg ttgaggtttt tgtagtcgtg ccacatgtag gggcgattgg gcaaatacag
     1381 attgtagcca tgcgtccaca aacgggtggc ataggtgatt tcctcgccat gaaaatagat
     1441 gtaagggtcg tatggcactt gtttgaacat ctcagctcgg ccgaacaaac tgcctgcggc
     1501 cacaaaagcg gtttgtgcac aacgcttggg agcggcctgt aagtccactg cgcctgaagt
     1561 cacttcgggc aattgggtgg ggagaaagct ttgcacatta aagtaagtgt acattaaggg
     1621 gtcttgctca ccggtttcgg tatcaaaccc catgggataa tgggtgatga cggcccgtgc
     1681 atcgccaatc tcggcataca tttgcaacaa acgcgtgtcc caatgcggtg caaaacggct
     1741 gtgactgtcg atttgcaagg caaaatcctg ttctgccagc agtgttttcc acacataact
     1801 gcgcgcccag caagctcctt gtgcctcatt gtaatcaagc acgtgttgca acacatttgc
     1861 cctctcaggc gccacacaat cttggtcttc ttgcagatgg atttgattga acaccccaac
     1921 atacacccgc tctgggtatt gtgcctgtgc aaacaaagaa gccagcgtca gcggcaactg
     1981 cggatcacga taggcggcga tggcaacaaa aatggtggct tcagataggg atttcatcat
     2041 gaggacgtga ggacaaattc agttggtgta agacagtaag tgcatcttag ctcataaaaa
     2101 tcagtccatc caagccttgt gtttgcacac gcatcagagt acgagccaaa ttgactgagc
     2161 tatcatccat ctttgggtat actgatgcca taatggcgca tgagcgatgc aatattgccc
     2221 gtccccaatc agaggaaaac catgtcattg aatcaacaac aaatcaccgc actcttattg
     2281 caacacttcc cgcaaattca agatgaaatc agcatagaca gcctaaccga gcatgaggcg
     2341 attgtgcgtt tgcgcgtgaa caaccgccat ctgcgtcctg gcaataccgt gtctggcccg
     2401 tctatgtttt tattggctga tgtggcagct tacattgcca tattggcgca agtcggggca
     2461 cgcacccaag ccgttaccac ttctgctcac atcgattttt tgcgcaaacc acaggctcag
     2521 gccgacatca ttgccaccac caaagtattg aaaattggca agcaattggc cgtaaccgat
     2581 gtgtatttgt attctgagaa tgacagcaat atggtggcgc gtgccgccat gacttattca
     2641 ctgccgcctg tgaatcaagc gtgattgaca gagcttgtga gcgtaccacg gcttaagaca
     2701 gtggtatggg tatgatcggc ctgtaattct gaacgcagca attgcaaagc cgcttcgatg
     2761 cgatgatggc cgcacatgaa gatatcgatg gcggcatacg cattttcagg ccaagtgtgt
     2821 atgctcaggt gcgactctgc caacaacaac acccctgtca ccccaccttc accgccaaaa
     2881 gtatgaaaat gactgcccaa aatggttgca tcagcggcca aggccacccg cgtgagcaaa
     2941 gcctcaatct gctttgcatc tcgtgcccac tggcaatcaa tgccgtatgc atcaaataga
     3001 caatgttgac ccattgcttg catgtactga ccttatttgt gaaaactacc gccacccgtg
     3061 cggataatgc tgctgccacc cgaactgctg cggctggaat caatcaccat ccaataattc
     3121 agaccagtgc tgatcaccaa caaggcaatg gcataccaag aaaaaaaacc tttcacgtgt
     3181 tccccttact cttgattgtt aaacggtaag ttcaacagcc aatacacaat aaaactgatg
     3241 atgaccaccc caaaaaaacc atcgccaacc aagcccatta acatggcagg gaaattcaac
     3301 accccataaa tcaacaacca aatcaacggc gaggccgctc gaccgccacc attttgttct
     3361 gccttggtgc gcaatttatc agccatattg ccgttgaaat gactggcctc caaacgcaag
     3421 gcttgtctcg ctaactgatc ggccgcagac aattccattg gtttattgga aatactggac
     3481 tgcttgaacc aagccgccac agcatgtgct ggaatttcgg taatggccga ccaagactgc
     3541 tcttcgcgca tcaatgccgt agacaatttc tgcttttcac gcccaaaatc gacataataa
     3601 gtggtgtctt tcggccctac ctgccaataa aaagccccag ttgcatattg cacttggcca
     3661 ccatagtcat acaactctgg tacctgccga ttgttggtgt ctacaggccg caatggttgc
     3721 tgtagcgttg gccaaacatc caaagatttg gcaattgccc agcggttacc agacaattcc
     3781 gtcagccaca aaaaaccttc tttgggcgag tacaacaggt attcaaacca cggttctccc
     3841 gcaggcacca atactttggg cgcattgtaa gagaatgcgg cttggctggc ttcatcccct
     3901 cgcacttccg attgcttcat ggccccaatc acccaccaat ctatggccat gatgcgtgcc
     3961 ttgctaccaa tcttaatcgt caatgcttca tcttgagccg cgcgcatgtc attggcagcc
     4021 accaatttga cttggccttc gttgaactca atttggcttt ggcaatattc acacactaca
     4081 tgcgtcgcca caccggttac ccagcgtact gggccaccac aattgggaca tttggattgt
     4141 tgggcactgc ctttaagctt gcccgcactg tctttgattt gctgcaaatc acgggtgttt
     4201 tgcaagcgta aggcattgag ggtaacacct tcaccgatga agacttcagg cttgttttca
     4261 tcttgtgtgt aatccaaagt catgaaaacg tcggcactgc gacagtcaac cacacgagcc
     4321 gtttcgtgct caggcaccaa atacggcaac tcgccttcag ccgcaaaacg cccccgactg
     4381 acctccctca cgtctgcagc cgcatacttt ttaggcccat aagcaaaaaa agtgcggcca
     4441 attttcatgt cagcatattt gggaatgatg ccgatttgtg ccttggtttc caaacgggta
     4501 aacacgaagc ggtctgaaga ctcagacaac caggcactgc tgccatcggc catcaaggca
     4561 tgccattcat tccaactgcc accatcgtat tgcacttgca agcggccgat taaggtgaaa
     4621 ggtgcccctt tccaagagcc actggtgcct atctgtaagg gtgaaaaatc gcggagcaca
     4681 gctgagtgac ggccagactc ggttaatgct tgaccctcaa gaattaacat cgaatggcaa
     4741 tagccacaga cttccgttac tgcggtcgcc gaatggattt caatttcagc gccgcagctg
     4801 ggacaaggag catggaacaa actggccaca tcaacccttt aaaaattatc cagtgatttt
     4861 tttgatgact tcggctttgg ccgtatcgta atcggcttgg ctgatgagcc cttggtctaa
     4921 caaggttttc aattgtgtca gcttgcccgc caaatcctca cctgcggcag gtgcagtcgc
     4981 agcaacagct gccgcagcca ctggggcagc ggtttgagcg gcctgtacca ctggctggcc
     5041 ttgcggtgcg acttgctggc ccatcatcgc ggcagtcatc gcattgccca tgcccatacc
     5101 cgccgccaaa ccggcaccga tgccggccac accaccttcg ttttgcgcgg caatcgggat
     5161 ggcttgtgcg gtttggtatt gggtatagcg gctcaaatcg cccacgatgc ccatgcccat
     5221 gcgttcgtcc atgcgttttt gcaaggcctc tggcaaggtc acgctttcca cattgaagct
     5281 ttccaacaac aagcccatgg cggcaaacaa gggctgcaac aattcagtca ttttttgcga
     5341 caaagccacc tggttggcag ccatgtccaa aaatggaatg cctgacgtac caaacgccgt
     5401 ggccaattgg gttaccgcca cattgcgcaa ttgttcttcc aattgctcgc cgcggtattc
     5461 tgcggtcaca cctgttactt ctttaaagaa taaagcaggg tcggcaatgc ggaaggaata
     5521 catgccaaat gcacgcaaag ccaccgcacc aaaatcggca tcacgcaccg ttactggctg
     5581 tgaagtgccc caacgacgcg ataattgttg gcgcaagttg aagaaataca catccgattt
     5641 aaacggcgac tcgaataatt ttgaccagtt ttttaagttg gttaatacag gtaagttttg
     5701 ggtggtgagt ttgaatgtac cggcctgaaa cacgtcagcg gtgacacctt cgtccacaaa
     5761 cattgctgct tgcgaatcac gtacggtcaa actggcgcca ttttgaattt cttcatcttg
     5821 aatcgggaaa cgccacatca agacttctgg ttcaggattc ggccattgaa tgacatcaat
     5881 gaattgcttt ttaataaatg atttaaacac catggtcttc agtcctttta actcaaagat
     5941 gcggcattca aaatgccgat agatacagac aatccaaaaa acaacactcc aacagcgaca
     6001 ttgttgtttt gcaattcagc attggcttct ggaatcaaat gggtggctgc aaaatacacc
     6061 aaaatttgaa tcaccgccgc ggcaacaccc caaatcacaa aactcaacat gccaacagag
     6121 tgcaacatgc tcgacaccaa agtgatgcaa aaacctatca tcgcaccact taaagacagg
     6181 gcacaggcca cattgccatt tttaatcaac tccaactcac gtgcaggcgt gattttcaca
     6241 tacgcaaaag caaacaccac cagcatcacc aaagcgacgc caaaatactt taaatactgt
     6301 aaatattgcc aaactgcgat gtccatggtt gtacccgtgt ctgtgtatta agcctaaatg
     6361 taacgtaaaa cctcagtttt gtacatgtgc aaaacccata cccacatgct cttatcatta
     6421 catgacaaag tgatgataag agtgggcaaa gccgcaagca ataacagttt gtgttagcat
     6481 gcacccactt gagtttttgg actatgtgtt gtgaatccag cgctgattgt ttcggtattt
     6541 attgttgcca gttgtggctt ggcctatgag ttaatcatgg cggcactcgc cagctatttg
     6601 ctcggcgatt caattttgca attctcgtcc atcatcggcc tgtatttgtt tgccatgggc
     6661 ataggcgccc acctcacccg ctacatcaaa gacgaagacg cgttgatgcg cttcatcgaa
     6721 atcgaaattt tggtcggcgt catcggtggc atttccgcac tggctttgtt tgtgttgttt
     6781 ggtttttcag ccgcaccgtt tcgcatcatt ttgtatggtt tggtgctgat tgttggcacg
     6841 gtagtaggca tggaaatccc tttggtcatg cgggtactca accagcacaa agccgacttt
     6901 aaagaaatcg tcagccgtgt actcacgttt gattacttgg gcgctttggc cgtgtctttg
     6961 ttgtttccct tggtattggc tccgaaactg ggcatggcac gttcggcctt gttattcggc
     7021 atcctcaacg tggccgtggc cttgattacc gcacgcatct ttaaacgtga cttaccaaaa
     7081 tacaaccgct tgcgctggcg cggtggcttg gtaatgttgg ccttgctcgc cagtttttgg
     7141 tatgccgaca acatcactta caaagccgaa caaaattatt ttggcgatcc catcgtctac
     7201 tccagctcct cgccttacca acgcttggtg gtgaccaaat ggcacaacga cacgcgcctg
     7261 ttcatcaatg gcaatttgca attttcttcc gccgatgaag cgcgctacca cgaagccttg
     7321 gtcttgcctg tgatggagca attgcctgag gtcaaagaag tattgatttt gggcggaggt
     7381 gacggtttgg ccgcgcgtga agtgttgaaa tacccgcaag tagaacacat taccttggtc
     7441 gatctagatc ccgaaatgac caaacttttt cgcacttcca aagaattaag cggcctgaac
     7501 caacaatcgc tgaacaattc caaagtcaaa atttacaaca ccgacgcggc caaatggctg
     7561 gaaacctcgc gccaacaatt tgatgtgatc atcatcgatt tgccagaccc gtccaacttc
     7621 tcgctcggca aactctactc cgtaccgatg taccgcatgg tcaaacgcca cctcaaaaca
     7681 ggtggcaaaa ttgtggtgca atccacctcg ccgtatttcg cgcctcaagc ctattggtgc
     7741 atcgtcacca ccttggaaac agccggcctg aaaaccttgc cctaccatgc ttacgtgccc
     7801 tcttttggcg aatggggtta tgtgatggca tcgctgcaag acgatttcaa agttccaacg
     7861 gcttaccaag taccgacccg tttcttggac aaaaccacca ctgcacaaat gtttgtcttc
     7921 cccaaagaca tgccacgctt gaatgtggag gccaaccatt tgaacacaca aaaactggtg
     7981 gagtattttg aatccgactg gcgtgcggtg atgcgctgat gatgaaactc agtcgccgcc
     8041 gttttcttgg ccatgtgttg ggcgcaggct tggtcagcgc cgccgcttac ggcacttacc
     8101 gtggttttgt gccaggctac cccaagattc atctcgatta tcctggcatg gatttgggtc
     8161 atgccatgcg cgatgcctta atcaagcggc ctgtaaacat acctagcacc gatgtcaacg
     8221 tcttgattgc aggcagcggt ggtgcaggct tgtcggcagc atgggcattg tccaaacaag
     8281 gcatgcacga ctatgtggta ttggatgggc ctgagcgcaa tggcaacaat cgcggcgagc
     8341 acaattacgg cgtgacctat cccacaggcg cgcattacct gcctttgccc gcagcagaat
     8401 ccacccacat ccaagccttg cttggtgact tggacatcta tcaaaatggc cgatacgacg
     8461 aaagcgccat ggtcaatgca ccgagcgaac ggctgtatta ccacaacaaa tggcaacccg
     8521 aaatcattcc cgaccacgat gccgacagcg agcgcttttt tcgccacatg cacactttaa
     8581 gcgcgcagcg cggcagcgat ggcaaacggc tgtttgtcat gcccatcacc ctctccagca
     8641 tggatgccac ttggcgcgcg ctcgaccaaa tcaccttcgc cacttggctg caacagcaac
     8701 actaccaaag tgaatcgctg ttgtggtatt gcgattattg ctgccgcgac gattacggcc
     8761 aaggcataca ccaagtgtcg gcatgggcag gtttgcatta cttttgcgcg cgcaacagcg
     8821 atggcaaaga cggccacacc gtattgactt ggccgcaagg tttaaacgag ttatcagaac
     8881 gcatgcgccg ctattgcaag ctgcaaaacc atgccgatgt ccacacttac aggccgcttg
     8941 tgcacacgcc cactgcattg tcggcaagcg ccttgaacat agaagaacac gcacaaggtg
     9001 tgcgggtatt gtgccgcgat ggcaaaggca atctgcgcta cctcaatgcc caaaaagtca
     9061 tttgcgccat gccgctctac atcgccgcgc gcgtggtgaa caacattcag caatacggtt
     9121 tcgaacccca acaacacttg ccagaatacg ccccatggtt ggtgagcaat ttcattttaa
     9181 aaggctttcc caccgaaaag tcaggcgcac cgttggcttg ggacaatgtc gcctaccaag
     9241 gccaaggctt gggctttgta ttggctacgc accaagaaat ttggcgctca ccgccgcctt
     9301 atacctcatt caccgcctac aatgccttga atcacgacag cccacaggcg atacgccatt
     9361 ggctggtcaa cagcagcccc gaagccattt accaacatgc agccgccgaa atcgaacaca
     9421 tgtatggcct gcaattgcgc cgccacatga tcgaggcccg catcaccgtg cgcggtcatg
     9481 ccatggcagc gcccacgccg caatacttga acaactctgg tttgcaagcc ttgcaaaacc
     9541 atacaggccg cttgtgcttt gcccacagcg atttgtcggg ctattcggtg tttgaagagg
     9601 cttgttggtg ggggcaagct gcggctgaaa aaatcttgcg accagcctga atgtatggtc
     9661 tctctagttt tattgagaag caatgactgc gtccatcttt aatgtgcgag aatcccaagc
     9721 catattgcgt ttaagtcggg aaaaccagca aatccgctta aattcaagaa atgttgactg
     9781 caatttcaat ctgattcaaa ccctgaacca atcaggattt cagccaaata ccaaccccat
     9841 tgcgcaatct aaataagttg ccagatggcc caatccaaag tatcgtgtcg aaactttaat
     9901 ctggagatag catcatgact aaattaagta ttttgagttt gtgcgccgtt ttggctttgg
     9961 cagcttgtgg cggcggcgaa aaagcagcca gcgacaaacc tgctgattca acagcaacca
    10021 cgtctactgc acctgctgag ccagcaaaag caggcggcga tccagctttg gacaaagaaa
    10081 ttgatgcagg cttggcacaa gttaaaggcc aattgccgat gaaattgggc gacaccatgg
    10141 aaatgacttc tatggaacgc aacggtactg acatcgtata cacctacact ttcttgaccg
    10201 acgcggtgaa aaaagacaac ttcaaagtag aagatggtaa aaaagccggt gcacaacaat
    10261 tgtgtgccaa tgctgaaacc aaaaaatact tggatgcagg ctacagcttc aacttcaatt
    10321 acaaattcaa agacggttct aacttagaag tgccgttcaa agcaggtgat tgctgattca
    10381 gccattcaaa aaaacagcca ctgaatgtgg ctgttttttt atttacaggc cgttttaaaa
    10441 caatgagaac aaaggacaga taatgagtat aaaaaatggt ttggcagtgt gtggctgcat
    10501 ccttgccctc agtgcttgtg attatttcgg gaaaggcagc aatgaagccc aaaaccaatc
    10561 aggcccgagc cgtaaacttg ataccttaat cgatgcagag ttggccgcca tgcgcctttc
    10621 tttgccacaa aaaatggctg acgaatttga cctgaccgat actcgccgtg taggtaaaga
    10681 tgtgacatac acttaccaat ttcataccca agtgaacaaa gccagcaatt ttgacgtgga
    10741 agcgggtaaa aaagtagcca tccccgaatt gtgcgatacc aaagccaccc gcgaatactt
    10801 ggatgcaggc taccgcttcg tgttcaccta tttgtttaaa gatggctcag atgtggtggt
    10861 gaaaatcgtc gctgccgatt gcaagtaaac ctcccaccgt gcctcgcctt tccccaagcc
    10921 attacaggcc gctgatatca ctgtatttgc tgtaaatact gctgcaattg atcgggtgtg
    10981 agctcaccca agctgtgcgc caccacctca ccctgctggt tcaaaaaata cgtgctgggc
    11041 aagccttggt atggcaacac tgttctggac tgcatggtct catctagcca aacgttagcc
    11101 agctgtattt ggtttttttg taagtatgcc gagattactg gggctttttc accttgattc
    11161 agcaacacaa attgaatgtg gggatggttt tgttgtgctt gttgcaacac tggcatttca
    11221 cggcggcaag gggcacacca tgttgcccac atattgatga cagcgggttt accctgcaac
    11281 tgttgcaaac tcactttctt accatgtaag tcttgcaaca ccagctctgg cttgggtttc
    11341 gattcaccac acgccaccaa taacaaggcc gctattgccc aaacacacgc ctgaattttt
    11401 ttcacttact cgctacctca gccaccgata caccccactc atcacaatta tttacctggt
    11461 ttgcaacgtt ttacgtggat tcattttgat gaaaacagaa ttgccgtata ataacaccat
    11521 tcgatgtcgc tgttttttat aaggataaca ccatgaaaca tgtcatcagc ttagccgttt
    11581 tactggccct cagcacagtt gccatggcca ataacaatcc tgcccagcgt tgcaagcgct
    11641 atgcagaagc caatgctttt caatccaccg tcgctgaact gtgcggaggc aacacccaat
    11701 cagagttttc aggggtgatg aaaggccaag cgtgcgaaga aactgtgggc aaggaaaaat
    11761 tgaaaaccca aggcagcacc caaagcgatg aattgaaagc cgaatacaac cgcatcggtc
    11821 acacccagtt ttgcagcaaa tatgcaggcc gtaaataaca cttgaagttt atggcccagc
    11881 cctcattttc acattgaggc acccacactt agtaaaggaa acaccatgaa acatttgtta
    11941 ggtagcttat tggcagttgc cgcgtttgcc agcagttctt ttgcatacgc tgatgcagct
    12001 tgctccaatt tggcgcgtga tttgtattcc gcccagggtt accagcaacg ctgtggttac
    12061 caattgaagc aaacacccaa tttgtcgaca caatttaaaa cacaaaaatg tgaccaaagc
    12121 ctctctggcg ccgacaaaaa caaaatgctc aatgaagtga acaacacctt gaatcaaagt
    12181 ttcaacgctt ccaaaagcaa aagctgcgat gaaggcaaat cacgcttcaa ttaattctct
    12241 cacttcaatg taaacaggag gctgtacatc cgcctccttt gtttacagga cgctttttat
    12301 gttttctcgc atcttgttgg caagcgcatt gttagccttg ccactcgtct cttttgcaca
    12361 aaccccacct tgtatgaatt tgctcaccca atccgcccaa gcaggcgtgg cagcgaaagt
    12421 gtgtcaaaaa caagtgaaca tggaagccat cgcccaattg caccagcaaa accaatgtgc
    12481 ggctttcttt gcccaagata aggtcaaaaa ccaaatcaac caagtggcca gtcaagccag
    12541 ccaccaagcg gcgcaagaag cacaacaact gggcagccaa cgctattgcc aacaagccgc
    12601 cgtcaactta ggcagtttat tgaaataaag cgccacactc atggtatgct caaacctctg
    12661 tgttacacag gagcaaacca tgtggcgtat tcctaccatc atcgcattgg gcttaggtct
    12721 tggcttcagc aaccaaactt gggccgacaa tgaccgcaac acctgcagcc aactggccat
    12781 aggcataggt cagtttgaaa aagtgcgcac cgtgtgtgac acgggcaaca tcgatgccac
    12841 attgtggcag agccaattga aatcaaatca gtgcagtcag gtattgggcg acgatgtgct
    12901 gacccaaatc atcaacaccg cccacaatga tgcgggtgcc accgcagaaa ccaaaggccg
    12961 cacccaatac tgcgaacaag atggcttgca gttattgcaa caactcagca gccccatgta
    13021 aacatgcaaa taaaagcggc ctgtaaatgc ttacaggccg cttttcacat ctgtttaagc
    13081 ttaaccgaat actttaaagc ggtcagcgcg agcgataaag gttttaggct ctgcggcttg
    13141 ttcgatggaa ccaaatggca tttgcgcacg caaagtccaa gaagcaggca agttccaagc
    13201 agcggcaacg gcattgtcta tcaatgggtt gtaatgttgc aaggttgcac ccaagcccaa
    13261 ttcagccaaa gcagtccaca ccatgaattg cgccatgcct gcgtgttgct cagaaaactc
    13321 tggaaatttg tcggcataca atgggaattg tgcttgttgg cctttgatga cttcttggtc
    13381 ttcaaagaac aaaacagtgc ccacaccagc agcgaaacag ccatcaattt tgccttcagt
    13441 ttgggcaaat tgttcggcag gaatcatagg acgcaaagtg tctttcacaa tattccacaa
    13501 tttttgatgc tcgtaaccaa acaacaccac cacacgcgaa gattgtgcat ggaaggctga
    13561 agggctttgc tctaccacct cttcaatcac gttcacaatc tcttcttgag agagactcac
    13621 gtttttacca atgttgtaaa tagaacgacg gcggctgatg ctttccaaaa agtttgctac
    13681 gctcatgttg tttcctttga tgtattgaat gttttgatgc ttcgcagtat agcgacttca
    13741 tgccagcact tcattagcga aacgggcaat gaagttttac tgacagtaaa caatgccgca
    13801 aggagtagcg tcttttgcgc cgttgtaaat caacaagaca cagtcaagca tgcttggcta
    13861 tactgatgag cagctgactc aacacccaac cataccaagc aagggcaaaa catggccaag
    13921 ttccagtcca aacacatcaa gatggcagtg ggcggcattt taatcgcgac tgttgccata
    13981 ggcggttggc gctttgccca aaaccgcatg ggctcattca gccaagtgga tgccatccac
    14041 atcgacatgg ccaagcctga tgtggtgata cagtccgaca gcctgaatca attgccgagc
    14101 aaattgctca ccattcccgt catcaaagac atcctcaccc aagaggtggt gttttactac
    14161 caagaagcgc ctgccaaaca gtctttacag ggcgcattac agcgcttggc tttcgaccac
    14221 aacctgcgtt ttgaagaccg catcatagac aaaatgctcg cgcgcaaagg cgatttgtac
    14281 gtgtggaaag gccacaccaa caagcccgac cattggctgt ttgttggcaa cagcgatgcc
    14341 tttagcaaat tttccgaagt attggccaaa gtcgccttag acgacagcca actcatacaa
    14401 gtggacactc tcaaagtcga tggcaacaaa attccgctgt acgccctcac ttaccagggc
    14461 aaaacaggct tgtttgccgc cacgtctgaa cgggtggtgt ttttatccga tgcaggcatg
    14521 ttgatggatt taaaatccaa gcctgccaaa gagctgcaat tggaagccaa agtcgatacc
    14581 gacaaagacg gcaatgaaaa agtcacgcca ttgcccatgt ccaaagccga aaagccagaa
    14641 ggcaagctga tttctgaacg cgccgacatg gtggcaaaac tcttgagcaa gcggcctgaa
    14701 aaccaacaag ccatcaatca aggtctgggt ctgagcaaag cggctcaaca cccacactcg
    14761 gtgtatttct ccgccgccat gctcaccctc tcttaccaag acttcatggg tggctttcaa
    14821 ggcagccgtt ttgaatttga tggcaataca tggcaacaag ccatcgcact caaacccagc
    14881 ttgctgccca atcacgaatg gaatggtacg gatttgtttg cccacatgcc gcacaatgcc
    14941 gccttttgtg ccaacgcccc catagagtgg ggcagcctca atacccaagc caaaaaattg
    15001 cccaccgcca ccgatgcctt gaatttggcg caattcagca gccccgtact ggcctgctgg
    15061 tatgcaggca gccctgctgc cgccccattg tttgtgacca agctgcccaa aaccgagcag
    15121 cgcgaagcgc aattggcggc cttgcaacaa gtgttcagcg atgtcattgg cgcgaaagaa
    15181 tacacccaca aacaacgctt tgaagtcagc cgcaaagaaa cacaaggcgt ggtggtgaat
    15241 gagcgcatcg tcagcgcccg ctatggcagc cacgacatag acgatttgcc cgcagacacg
    15301 gccaaagaat tgtcggccac aagctacttc cctgtgcgct atgctgtgag tggggattat
    15361 gtgtggtttt cgcccaatgg cgatttggtg gagcaagccg tggccgtgag ccaacaacaa
    15421 gcgcctgcgc tggccgaaca aatacagcaa caaaattctg ctttgctgtg ggtaaatggt
    15481 gcggaattgg ccaaatggct gcaacagcga accagcgcag tcatcgccga agacagcgac
    15541 atgcgcaccg tattgtcgca acgcctcacg cccaaactgc gcctcatcag cagcagtggc
    15601 tcatggcaag tacaggccga taccaagggt ctgaatccaa gcagcaataa cactcaatgg
    15661 gtcaacgtga catggcaaca cctcccttaa gccgccgcga ttgcttggct tatttggcgg
    15721 cgctcagcct tgccgctcct gctcaagcac tcagtggctt ggccgaagcc aaaaccagcg
    15781 cccgcttgga tgccgagcaa agccgcctgt ttcgcctgtg gtttgccgcc atcgtgcgcc
    15841 aacaagtggc acaagggcca tcggtgcgtt ggcaacaccg cgattgtgcg ggcttggtac
    15901 gctttgcggt gtttgaaacc ttgcgcagcc acgatgcggc
//
LOCUS       DAOMVK010000006        14307 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_926_length_14307_cov_2.828094, whole genome shotgun sequence.
ACCESSION   DAOMVK010000006 DAOMVK010000000
VERSION     DAOMVK010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 14307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14307
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_926_length_14307_cov_2.828094"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(546..1046)
                     /locus_tag="PK856_00550"
     CDS             complement(546..1046)
                     /locus_tag="PK856_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003793332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HRL00892.1"
                     /translation="MKTLQQGFTLIELMIVIAIIGILAAIALPMYQDYISKSQVTRVY
                     GELAAAKTAVDSAKFENKTNALGTNSTDANVVTMGLSTGAETTTATGTQTRSNLVDNL
                     TLDQPADNTVTYTARFGRNANTSIAGATISLSRDTNGVWSCRTTAGTATAGWKPKFSP
                     TGCANS"
     gene            complement(1156..1638)
                     /locus_tag="PK856_00555"
     CDS             complement(1156..1638)
                     /locus_tag="PK856_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HRL00893.1"
                     /translation="MKTKIRGFTLIELMIVIAIIGILAAIALPLYQDYIIKSQLTRVN
                     YELASVKTAVETVIANGNIPTLTPNEDGMPFMGKKLEYIGLNKDNPQSNLIYNLNILN
                     NQPNYMIYASMNENAVATVKDVVFIYTREFSGVWTCEVDISSASTWKKSYVPNGCTEI
                     "
     assembly_gap    1821..1830
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1831..4422)
                     /gene="glnE"
                     /locus_tag="PK856_00560"
     CDS             complement(<1831..4422)
                     /gene="glnE"
                     /locus_tag="PK856_00560"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HRL00894.1"
                     /translation="MTSAYLQQARPFSLYLQRQLDNQRLNLNLMETWLSRPLNLDDFA
                     NFAPWPMLIENRDEEEMSRQLRILRRHVVCQIMVRDLCRLAPLSEVTSSITLFADFAV
                     NTALVYAENHYEHMYGKPLGAFTGNEQHLTVVGMGKMGGYELNVSSDIDLIFVFPEAG
                     DTNGKRPKSNVEFFTKVGQKTISLLNDITAEGQVFRVDMRLRPDGDSGALVLSEAALE
                     RYLITQGREWERYAWIKGRIISPGINDITSLVRPFVYRKYLDFDAYEGMRSLHNQIRQ
                     EVSKKGMQDNIKLGAGGIREVEFIAQVFQLIRGGQVRQLQRKDTQGTLALLATLHLID
                     ASTSEQLLCAYRFLRDTEHRLQYWDDQQTQTLPESSESQFKLAQSMGFREYSDFQAAL
                     NQHRGFVNQVFNQILGDTPSNPNSKIDTEYHAMWQEDINDELLAERLTANGFEATPIL
                     ERLNAIRHSSRYRHLNNTAHTRLNKLMPYLFAAAADTEDATKTVIRLIDFIDTIIRRS
                     SYLAFLHEKPEALKRLAHIVGNSSWMASYLSRHPILLDDLLSSQLRIKEFDWQAACKQ
                     LENNLNACHGDVEAQMDALRHYQHSQVFRLAVQDLEGMWAVEALSDELSALADCILDC
                     TVMQLWKSLKNTHTDVPKIGIIGYGKLGGKELGYASDLDLVFVYNDSHPDAASNYGRL
                     FRRLITWVSTTTAAGGLYEVDVRLRPDGDAGQTCVSLEAFKKYQLEKAWTWEHQALTR
                     ARFISGTEEVALGFEEIRHAILTQQRDLRKLQTEIVAMREKMLPTHPSKEENVKYARG
                     GIVDVEFIVQYLILAHSHIFPQLTQNYGNIALLGIAADLGLIDLNLARKSQEAYRYYR
                     KVQHLSNL"
     gene            4512..5318
                     /locus_tag="PK856_00565"
     CDS             4512..5318
                     /locus_tag="PK856_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HRL00895.1"
                     /translation="MSMHIKVAAIQMVSTTNLEQNMATAERLLGQAADQGVQWALLPE
                     YWPIMGRKDTDKLAIAEPFGQGVLQTFLAATAKRLGMVIIGGTIPLQSEVENKVLNTM
                     LTYQRDGELIGRYDKMHLFGFQGLGESYQESDTIQAGSDVPQLSIDGIGVAQGICYDV
                     RFPEFFRAQLPFDVMVLPAAFTHTTGQAHWELLLRARAVENQCYVLASGQGGVHESGR
                     QTWGQSMLIDPWGNIVNQLANHEGVVIGDIDTQKVQSVRSKLPALRHRCL"
     gene            5647..6429
                     /gene="yaaA"
                     /locus_tag="PK856_00570"
     CDS             5647..6429
                     /gene="yaaA"
                     /locus_tag="PK856_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HRL00896.1"
                     /translation="MYFVISPAKNLDETSPVSVNEHTAMALPEDTQILMGVLQGLSPQ
                     DLSALMGVSDKIARLNVERNGQFQVPVTLDNGGKQALFLFKGDVYAGIDVYHLSANAL
                     QYLQQHLGMLSGLYGLLRPFDLMQAYRLEMGTALKNPRGDNLYQFWGSKITQLINQRL
                     QVAEADYLINLASQEYFKSVQAADVIKPIITPVFQDEKNGKYKIISFYAKRARGLMVK
                     FTAENQISDAEQLKEFNLEGYEYSESDSSSNTWVFKREERQK"
     gene            6498..6588
                     /locus_tag="PK856_00575"
     tRNA            6498..6588
                     /locus_tag="PK856_00575"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6532..6534,aa:Ser,seq:tga)
     gene            7407..8291
                     /locus_tag="PK856_00580"
     CDS             7407..8291
                     /locus_tag="PK856_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRL00897.1"
                     /translation="MIERSHLTIIREVERQGSLTAAAETLCLTQSALSHSVRKLEHLL
                     GTAVWLREGRSLHLTQAGQYLLDLANRVLPQLERAEEQLQQYATGHRGTLRIGMECHP
                     CYEWLLRVVSPYLDAWPDVDVDVKQKFQFGGIGALFTHEIDLLVTPDPLYRPGLHFEA
                     VFDYEQVLVVSSAHALAGRDFVRPKDLLNEVVITYPVESSRLDVFTLFLNPANTMPKQ
                     HKTIETTDIMLQMVAGNRGVAALPRWLVETYQNRFDVVALSLGQAGIHKQIYLGAREK
                     DLHTEYMDGFFQLSRKPI"
     gene            complement(8386..8901)
                     /gene="coaD"
                     /locus_tag="PK856_00585"
     CDS             complement(8386..8901)
                     /gene="coaD"
                     /locus_tag="PK856_00585"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009427597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRL00898.1"
                     /translation="MAEKRIRRAVYAGSFDPPTNGHLWMIREAQMLFDELVVAIGVNP
                     DKRFTYNLEERKEMLRAITAGMSNVRVASFENQYLVNYADSIHAEYIVRGIRTTADYE
                     YERSMRYINSDLQPEISTVFLLPPREYAEVSSTMVKGMVGPKNWQEMIKRYLPEPVYE
                     KIMHDHLYQGR"
     gene            complement(8901..9626)
                     /locus_tag="PK856_00590"
     CDS             complement(8901..9626)
                     /locus_tag="PK856_00590"
                     /inference="COORDINATES: protein motif:HMM:NF005703.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRL00899.1"
                     /translation="MEIQSELGCGGFAFDMNVACSSATFAVQMAANAIAAGSAKRVLV
                     VNAEICTAHLNFRERDSHFIFGDAATALVLVHQDLLSPADAHKGFNIVDTSLWTQFSN
                     NIRNNAGFLNRTEVDNDPLATDKLFVQNGRKVFKEVCPAVGEHIVNHLHKNHIEPTQV
                     KRFWLHQANLTMNELISKRVLGRPATAEESPTILDTYANTSSAGSMIALHLHHADMQT
                     GDLGVLSSFGAGYSIGSVIVEKR"
     gene            complement(9578..10030)
                     /locus_tag="PK856_00595"
     CDS             complement(9578..10030)
                     /locus_tag="PK856_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00900.1"
                     /translation="MTHMPVVLLGTGLYTPKDSISNDELVATFNQYVNQYNQEHAAEI
                     AAGAIEALAESSSAFIEKASGIKSRFVMDKAGILNPDLMKPQFAPRSNEDLSLQAEIG
                     VAAAKQALGNAGVEAADIDMVIVANSNMQRAYPWKSNLNWVVAALRLT"
     gene            complement(10218..10898)
                     /locus_tag="PK856_00600"
     CDS             complement(10218..10898)
                     /locus_tag="PK856_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HRL00901.1"
                     /translation="MSEQPLAHGHTGVSVQNQVLRKAYLLLAFSFIPCGIGAFVGSNI
                     NPMAMLGGGWVGIIAFFAIFYGLIFAIEKNRYSNVGIVLLQVFTFGMGMMLGPILGML
                     LGSAAGTQIVVTAAVMTVAVFFAMTIAAHKVKTDTNALGRFLLIGAIVLMVGVVANLF
                     FKIPAVSLAISAMFVLFSSLMIMWQTKMVIEGGETSYISAALTIFISIYNLFTSLLRL
                     LMALTGND"
     gene            complement(11023..11727)
                     /locus_tag="PK856_00605"
     CDS             complement(11023..11727)
                     /locus_tag="PK856_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00902.1"
                     /translation="MARSKPFGTITTVLSTLSLCMGIPRLAVAEAPSFNCTKNLSAAE
                     SLICQDAKLSRLDRDLALSYKNVNQRLDGEGKAALLEEQRTFLYARDRLAEEIKSSNQ
                     ASKSSLGQLMRQHLSSLQHIKPRQQASNIMGSWFVTSGGVDINKRDANTLSLHLNRVE
                     PTRGNWVCDFQGTAQATDAQAKHLVFHSNDDPQQQLHIERVGSILRVSTPQSNGTNYC
                     GLNGTVNGDYLPLSSK"
     gene            complement(11797..>12416)
                     /locus_tag="PK856_00610"
     CDS             complement(11797..>12416)
                     /locus_tag="PK856_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein"
                     /protein_id="HRL00903.1"
                     /translation="DEDRLGNDVMEMLMNYEFTALKDISLSSIVNDITSIIRENNLTL
                     PGDLVMLFRTLITLEGVVKQLDGDFQLMESTKPLVEGLVKQHFSPDHMTRKVKTHTYS
                     LLQLLGDMPHTLIRLNRKLKAGKIQVNVDMERLDLFGYQIDKSTNRLTVGIITASLII
                     GSSIVMSVDAGPKIFGLPFFGFVGYVIAFFNSMWMLWSLWRAGKH"
     assembly_gap    12417..12459
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12460..13453)
                     /locus_tag="PK856_00615"
                     /pseudo
     CDS             complement(12460..13453)
                     /locus_tag="PK856_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313064.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
     gene            13555..14112
                     /locus_tag="PK856_00620"
     CDS             13555..14112
                     /locus_tag="PK856_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HRL00904.1"
                     /translation="MMSKTIHRLLVLVLSCISLVPIATADPLEDLIRQRGGSSNSSQA
                     QSQASKEQAGDLIMNAMGLIGLSYRYGGNNPNQGLDCSAFMQYIFKQSMRVSLPRTAS
                     EQFGVGSSVDRSSLQPGDMVFFRTAGPSRISHVGMYIGGDRFIHAPRTGKNIEITSIT
                     SKYWSKTYAGARRVSGAGKAASFVR"
     gene            14122..>14307
                     /locus_tag="PK856_00625"
     CDS             14122..>14307
                     /locus_tag="PK856_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00905.1"
                     /translation="MSEMPPMPKWFDAEGEPVSCTEKIKVMQQNLQELHEMAQEALED
                     AVLMGCTEAQVKDYLRVL"
BASE COUNT         3785 a         3013 c         3515 g         3941 t
ORIGIN      
        1 atagattacg agaaaattct acttaatgct gcacttattt aaagggggga ttaccattcc
       61 tagttatcta ggacagccct taatattaat ataagcgtaa aagatatatg tgggttgagt
      121 taggattttg aaagactaaa tgtttttgga ggtggagatt gatgcattct cttaagggtg
      181 aggccagatg ttttgaatga tgctgcttta atcttaataa aagaaatttt aagtactacg
      241 tgctcgtaat gaattatgaa tgttgggctt tcttaagata atagaacttt gttattggta
      301 tgaatagtaa agttataagt ggacccttaa atcaaataat caaggtattg taagcttgga
      361 ttaatgagct tgtaaatctc ataaaatatt tgaaagtagg ttttaaaata aaaatagatt
      421 attgattgca cccatgtaat ttatagtggt atttctatta atagtaaaga taatgtcttg
      481 attttgacta ttactaaaag aaaagacgct tggagtccaa gcgtctttag attgattacg
      541 tctaattaag agttagcgca accagtaggt gagaatttag gtttccaacc tgcagttgct
      601 gtgcctgcgg tagtgcggca tgaccaaacg ccattagtat cacggctcaa agagattgta
      661 gcacctgcaa tgcttgtatt agcattgcgg ccaaatctgg cggtataggt aacagtgtta
      721 tctgcaggtt gatctaaggt taagttgtca accaaatttg aacgagtttg agtaccagta
      781 gcagtagtgg tctctgcacc agtagataga cccatggtca caacattggc atctgtagaa
      841 tttgtaccca aagcatttgt tttgttttca aacttagcac tgtcaacagc tgtttttgca
      901 gcagctaact caccataaac acgagttact tgagatttag aaatgtaatc ttggtacata
      961 ggcagagcaa ttgctgccaa aataccaata attgcaatca caatcatcaa ttcgattaat
     1021 gtaaagcctt gttgtaatgt tttcatttgt ttactcctgg ttatttaaag aaaaatatat
     1081 tcggttattt tgccgaactt tcaaacttac ttggttaaag catttcccgt gccaagtaag
     1141 aaatcttaca tatatttaaa tctcagtaca tccattagga acatacgatt ttttccaagt
     1201 gctagcagag ctaatgtcta cttcacatgt ccatacccct gaaaattctc gggtataaat
     1261 gaatacgacg tccttgaccg ttgcaactgc attttcattc attgaagcat aaatcatata
     1321 gttgggctga ttgttcaaga tattgaggtt gtaaattaaa ttggactgag ggttgtcttt
     1381 atttaagcct atatattcca gttttttacc cataaaaggc ataccatctt catttggagt
     1441 gagcgttggt atgtttccat tcgctattac tgtttctacc gcagttttaa cagatgctaa
     1501 ttcataatta acacgtgtta actgagattt aataatgtaa tcttgataaa gaggcagtgc
     1561 aatcgcggct aatataccaa taattgcgat gactatcatc aattcaatta aagtaaagcc
     1621 acggattttt gttttcattt acacactcac aaggtagttt gttatttttt gtaaatgaaa
     1681 gcaatgtaaa tgcctttttg tatgacattt gcaaaaaaaa gacaaatggg cttcgtttgg
     1741 gtttaacggt ggtctgaaaa acataatttc catagactgt tcacatgctc atagtggctt
     1801 attagttcat tgttaatctc nnnnnnnnnn taaattgctc aagtgttgca ctttgcggta
     1861 gtaacgatac gcttcttggg attttcgggc tagatttaag tcaattaaac ccaaatcagc
     1921 ggctattccc agcagcgcta tattgccata attttgtgtc aattgtggga aaatatgcga
     1981 atgagctaaa attaagtatt gaacaatgaa ttccacatcc acaattccgc cgcgggcata
     2041 tttgacgttt tcctcttttg aaggatgggt gggcagcatt ttttcacgca tggcgacaat
     2101 ttcagtttgc agttttctta agtcgcgctg ctgcgtcaag atggcgtggc gtatctcttc
     2161 aaatcccagt gctacttctt ctgtgccgct aataaagcga gcgcgagtta aggcctggtg
     2221 ctcccaagtc catgcttttt cgagttggta tttcttaaag gcttcgagtg acacacaggt
     2281 ttgccctgca tcgccatctg gccgtaggcg cacatcaact tcgtacaggc cgcctgctgc
     2341 tgtggtggtt gagacccaag tgattaatct gcgaaagagg cgaccgtaat tggaggcagc
     2401 atcagggtgg gagtcgttgt aaacaaagac caggtctagg tctgaggcat agcccaactc
     2461 ttttcctccc agttttccgt atcctatgat gccaattttg ggcacgtcgg tatgggtgtt
     2521 tttaaggctt ttccaaagct gcatcacggt acagtcaagg atgcagtcgg cgagggcaga
     2581 gagctcatct gacaaggctt ctactgccca catcccttcc aaatcttgta cggccaaacg
     2641 aaaaacttgc gaatgctgat aatgacgcaa agcgtccatt tgtgcctcta catctccgtg
     2701 gcaagcatta aggttgtttt ccaattgttt acaggccgct tgccaatcaa attctttgat
     2761 tcgcaattgg ctgctgagta agtcatccag caagatgggg tggcggctta aataagatgc
     2821 catccaactg ctgttgccca caatgtgggc caagcgtttt aaagcttcgg gtttttcatg
     2881 tagaaaagcc aaatacgaag aacgtcggat gatcgtatca ataaaatcga tgagccgaat
     2941 cacggttttg gtggcatcct cggtatccgc tgccgctgca aacaaataag gcatcaattt
     3001 atttagccgt gtgtgagccg tattgtttaa atgacgatag cggctgctgt gacgtatggc
     3061 attgaggcgt tctaagatgg gtgtggcttc aaaaccattc gcggtcaatc gttcagccaa
     3121 aagctcatca ttgatgtctt cttgccacat ggcatggtat tcagtatcta ttttagaatt
     3181 gggattgctt ggggtatctc ccagaatttg gttgaaaacc tgattgacga accctcggtg
     3241 ttggttcaaa gcggcctgaa aatctgagta ttctctaaaa cccatgcttt gggccaattt
     3301 gaattgtgat tctgaagatt ctggcaaggt ttgggtttgt tggtcatccc aatactgtaa
     3361 gcgatgctcg gtatcgcgca aaaatcggta ggcacagagc agttgctcgc tggttgatgc
     3421 atcgattaaa tgcagcgtag ccaataatgc caaggtacct tgtgtgtctt tgcgttgtaa
     3481 ttggcgtacc tgcccgccac gtatcagttg aaacacttgg gcaatgaatt caacctcacg
     3541 tatgccgcca gctcccaatt taatgttgtc ttgcatgcct tttttgctga cttcttggcg
     3601 gatttggttg tgcaaggaac gcatgccttc atatgcatca aaatccaagt atttgcggta
     3661 aacaaaaggg cgtaccaagc tggtaatgtc attgatgcca gggctgatga tgcgtccttt
     3721 aatccaagca tagcgttccc attctcgccc ttgagtaatc aaatagcgct ccaaagcagc
     3781 ctcactcaat accaaagcgc cagaatcgcc atctggccgc aagcgcatgt ctacacgaaa
     3841 tacttgccct tcagcggtaa tgtcattgag gagagaaatg gttttttggc caactttggt
     3901 aaaaaattcc acattgcttt ttggacgttt gccattggta tcgcctgcct caggaaatac
     3961 aaatatcaaa tcgatgtctg acgacacgtt caattcatag ccgcccatct tgcccatgcc
     4021 aaccacagtt aggtgctgtt cattgcctgt aaaagcgccc agtggcttgc catacatgtg
     4081 ttcgtaatgg ttttcagcat acaccagcgc cgtgttcacg gcgaaatcag caaacaaagt
     4141 gatgctggat gtgacttcac ttaaaggggc taggcggcac aggtcgcgaa ccataatttg
     4201 gcagaccaca tgacggcgca gtatgcgcaa ttggcggctc atctcttctt cgtcgcggtt
     4261 ttctatcagc attggccatg gtgcaaaatt ggcaaagtca tccagattta atggccgact
     4321 gagccatgtt tccattaagt tcaggttcag acgttggttg tcgagttggc gttgtaaata
     4381 caaggaaaac gggcgggctt gttgcagata ggctgaagtc atggtgttgg gctgcacaaa
     4441 aaagacaatg gcagttatca tagctgcttc aatgcggcct gaacagtcag gcacatgcag
     4501 ccactgagga tatgagcatg cacatcaaag ttgcagcaat tcaaatggtg tccactacca
     4561 atcttgaaca aaacatggcc acagccgagc gcttgctggg gcaagccgct gatcaaggtg
     4621 ttcaatgggc gttgttgcca gagtattggc ccataatggg gcggaaagac acggataaat
     4681 tggctatagc tgaaccattt gggcaaggcg ttttgcaaac atttttagcg gctactgcca
     4741 aacgattggg catggtcatt attggtggca ccataccctt acaaagtgaa gttgaaaata
     4801 aggttttgaa taccatgctg acttatcaaa gggatggtga gctgattggt cgctatgaca
     4861 agatgcattt gtttggcttt caaggcttgg gtgagtctta tcaagagtcg gacacgatac
     4921 aggccggatc tgatgtgcct caattgagca tagatggcat cggtgtcgcg caaggcattt
     4981 gctacgatgt gcgtttccca gaattttttc gtgctcaatt gccatttgat gtgatggtgt
     5041 tgcccgccgc atttacccat accacgggcc aagcgcattg ggagctgctc ttgcgtgccc
     5101 gtgcagtaga gaaccaatgc tatgtgttgg ccagtgggca aggcggggtg catgagagcg
     5161 ggcgtcaaac ttggggacag tccatgctca tcgacccatg gggcaatata gtcaatcaat
     5221 tggccaacca cgaaggcgtg gtcataggcg acattgatac ccaaaaagta cagtcggtgc
     5281 gcagcaaatt gcctgcattg cgacaccgtt gcttgtgagg cggtttgatt gagcttcgga
     5341 aagcaagagc attgtaagca aataaagtaa tgagattcgc aatgcagaaa cttggcgatg
     5401 gcgttaaatg cggcctgtaa gtttttaact gagtgtgaaa atgcttgaga atcattttgc
     5461 aatattaaag atgccaatgc gcatgaaaag acatttaagg aatgctagag cgcttgtatg
     5521 aagcataggc taaaaaaaca agcggcctgt aaggttacag gccgcttaat gagtatgtaa
     5581 cagtgagtgg gcaaatttgt ttgataaaat gacatgccca gccaatttaa ggatgttgag
     5641 atgagtttgt attttgtgat ttcccctgcc aaaaatttgg atgagactag ccccgtgtca
     5701 gtaaatgaac acactgcgat ggcattgcca gaggatacgc agattttaat gggtgtttta
     5761 cagggtttgt caccacaaga tttgtctgcg ctgatgggtg tttctgacaa aattgcccgt
     5821 ttgaatgtcg agcgtaacgg gcaatttcaa gtgcccgtca ccttagacaa tggcggcaaa
     5881 caagcacttt tcttatttaa aggcgatgtg tatgcgggca tagatgttta ccatttgagc
     5941 gcaaatgcac tgcaatactt gcaacaacat ttgggtatgt tgtcagggtt atatggcttg
     6001 ttgcggcctt ttgatttgat gcaagcctac cgtttggaaa tgggtaccgc gcttaaaaat
     6061 ccccgtggcg acaacttgta tcagttttgg ggtagcaaga ttacccaatt gatcaaccag
     6121 cgtttacagg tcgctgaagc cgattatctg attaatttgg cttcgcaaga gtattttaaa
     6181 tcggtacaag ctgccgatgt catcaagccg attattaccc ctgtgttcca agatgagaaa
     6241 aatggtaaat acaaaatcat cagtttttac gccaaaagag cgcgcggcct gatggtgaag
     6301 ttcacagcgg aaaaccaaat cagtgatgca gagcaactca aagagtttaa cttagagggg
     6361 tatgagtatt cggaatctga ttcaagttca aatacttggg tatttaagcg cgaggagcga
     6421 caaaaataat gccgattttg tgcaaaaaag gcttgcgcga aaaaagatgt taacgtatta
     6481 tgcacttctc tcaagacgga agcgtggcag agcggttgaa tgcaacggtc ttgaaaaccg
     6541 tcgagggctt atacccctcc gtgagttcga atctcaccgc ttccgccaag taattaatta
     6601 aagccctgtt tatacagggc tttaatttta tctgtgtatt gtaaaagcgg ctgatctagc
     6661 tgatttacat attggttttg ttctacactt ttactctttt tctacatttc atatgttgtc
     6721 gctcttaaaa atcagtttta aatcactcac tagcaatgca gctacatatc ctttcttatt
     6781 tttagtgtaa tcactggcat gcaatgagat gcatagtcac gcaatgtgca gatagactca
     6841 ttacttatgg tgtcgttgta ttagttgcgg ttttgccgca tctctaaact ggaaataggt
     6901 ttttttagtt cttggcttgg tgcgagcgaa gccaaataaa gttctcagca agtatataga
     6961 tgacaagctt cctatttaga ttcaacaaat gattaggtgt tgctgattga agttaagact
     7021 taattgcctc catggctact tttaactaag caataagttg aatcctcacc atggcttgaa
     7081 ccattcttag gcaatccaca attgatacca gcgtgcaagc atcgggtggt aaatttttat
     7141 ggtgttggca taatttttcg acatatgttc taatggctgg gataattcag aatcatctct
     7201 gctgttcgtg tgtgtgcaca gagtgataaa aaagggaggc ctgtgagggt ctccttttcg
     7261 tttgtgtgac ataaagttgg atttgtttaa attgctgagt gattgaagag cggctttaat
     7321 gctctgtatg acggtattga aaatacttgg catgaattac aatacggtat tcttggtaat
     7381 caatgatacg tattcatggg tgaattatga tagaacgcag ccatttgacc attattcgtg
     7441 aagttgagcg gcaaggctcg cttacggccg cggctgaaac gctgtgcttg acccaatctg
     7501 cactcagcca cagtgtgcgc aagcttgaac atttactggg taccgcagtg tggctgcgcg
     7561 aggggcgcag cctgcatctg actcaagcgg ggcagtattt gttggatttg gccaatcgcg
     7621 tattgccaca attggagcgg gcagaggagc agttgcagca atatgccaca ggccatcgcg
     7681 gaacgctgcg cataggcatg gaatgtcatc cttgctatga gtggttgctg cgggtggtat
     7741 cgccgtattt ggatgcatgg ccagatgtgg atgtggatgt gaaacagaag tttcaatttg
     7801 gcggtattgg ggctttgttt acccatgaga ttgacttgtt ggttacgcct gatccgcttt
     7861 acaggccggg tttgcatttt gaagcggtgt ttgattatga gcaggtgttg gtggtgtcat
     7921 cggcccatgc tttggctggg cgtgactttg tcagacctaa ggatttattg aatgaagtgg
     7981 tgattaccta tcctgttgaa tccagccgct tggatgtgtt taccctgttt ttaaaccctg
     8041 ccaataccat gcccaaacaa cacaaaacca tagaaaccac cgacatcatg ttgcaaatgg
     8101 tagcgggcaa ccgcggggtg gctgctttgc ccagatggct ggtggagacg tatcaaaacc
     8161 gatttgatgt ggttgctttg tctttggggc aggctgggat acacaaacaa atctatttgg
     8221 gtgcgcggga aaaagatttg cacacagaat acatggatgg gttttttcaa ttgtcacgca
     8281 agccgatttg attgctgtgt cagtatggaa gtttattgag tttgcactgc taaaaaagcg
     8341 gcctgtaaat tacaggccgc ttgtaggtat gcttgctgcg cttcttcatc tgccttggta
     8401 caagtggtcg tgcatgattt tttcatacac aggttcaggc aaatagcgct taatcatttc
     8461 ttgccaattt ttcgggccca ccatgccttt aaccatggtc gaagacactt cagcatattc
     8521 gcgtggcggc aataggaata cggtggaaat ttcaggctgt aagtctgaat tgatgtagcg
     8581 catggagcgc tcgtattcgt agtcggcagt ggtgcgtatg ccgcgcacaa tgtactcagc
     8641 atgtatgctg tcagcgtagt tcaccaagta ttggttttca aacgaggcca cgcgcacatt
     8701 gctcatgccc gcggtaatcg cccgcagcat ttccttgcgc tcttctaagt tgtaagtaaa
     8761 gcgtttgtcg gggttcacac cgatggccac caccaattca tcaaacaaca tttgggcttc
     8821 acgtatcatc cataaatggc cattggtggg ggggtcgaag ctgcctgcgt aaaccgcgcg
     8881 gcgtatgcgt ttttctgcca tcagcgtttt tccacaatca cagaacctat ggaatagcca
     8941 gcaccaaagc tcgataacac acccaaatca ccagtttgca tgtcggcatg gtgcaaatgc
     9001 agggcaatca tagagcccgc tgagctggta ttggcgtaag tatccaatat ggtcggtgat
     9061 tcttccgcag tggctgggcg gcccaacaca cgtttggaaa tcaactcgtt catggtcaaa
     9121 ttggcttggt gcagccaaaa acgcttcact tgagtgggct caatgtgatt tttgtgcaag
     9181 tggttcacaa tgtgttcgcc cacggctggg cacacttctt tgaagacttt gcgaccgttt
     9241 tgcacaaaca atttgtcggt tgccagtggg tcattgtcca cttcggtacg gtttaggaaa
     9301 cctgcattgt tgcggatgtt gttggagaac tgcgtccaca gcgaggtgtc aacgatgtta
     9361 aagcctttgt gggcgtcggc agggctcagc aagtcttgat gtaccaatac caaagcggtg
     9421 gcggcatcgc caaagatgaa gtggctgtca cgctcacgga aattcaaatg ggcggtgcag
     9481 atttcggcat tcacaaccaa cacacgtttg gctgagcctg cggcaatcgc attggcagcc
     9541 atttgtacgg caaatgtcgc gctggagcag gctacgttca tgtcaaacgc aaagccgcca
     9601 caacccaatt cagattggat ttccacgggt aggcgcgttg catattggaa ttggcaacga
     9661 tgaccatatc gatgtcggca gcctctacgc ctgcattgcc caaagcttgt ttggccgcag
     9721 ccacaccaat ctcggcctgt aaagacaaat cttcattgct gcgcggcgca aactgaggtt
     9781 tcattaaatc tggattcagt atgcctgctt tgtccatcac aaaacggctt ttgatgcccg
     9841 aggctttttc aatgaaagca ctgctggatt cggccaaagc ttcaatggca ccagcagcaa
     9901 tttctgccgc atgctcttgg ttatactggt taacatattg gttaaatgtg gccaccaact
     9961 catcgttgct gatgctgtct ttgggggtgt acaaaccagt gccaagcaag actacaggca
    10021 tatgggtcat gggtgtggtt ctccaactga attcatcaag gcaaacagca acatagtttg
    10081 aatgctctga tggtgaatat ttgtgtatat agtgcacagc agcataactg ggttaatctg
    10141 ctcagtcaaa gaaaaatacc aaaaaaagcg gcctgtaaat cttacaggcc gcttatattt
    10201 agagaaaaaa cctagactta gtcgttgcct gttaaggcca ttaataagcg caacaagctg
    10261 gtgaataagt tgtaaatcga gatgaaaatg gtcaaagcgg cgctgatgta actggtttcg
    10321 ccgccttcaa tcaccatttt ggtttgccac atgatcatca atgagctgaa caacacaaac
    10381 atggctgaaa tggccaatga tacagctggg attttaaaga acaaattggc aaccacaccc
    10441 accatcaaca cgatggcacc gatgagcaag aagcggccca aggcattggt gtcggttttg
    10501 actttgtgag cagcaatagt catggcaaag aacaccgcta ctgtcatcac cgcagcggtc
    10561 accacgattt gggtgcccgc ggcagagccc aataacatgc ccaaaatcgg gcccaacatc
    10621 atgcccatgc caaaagtaaa cacttgtaat aacacgatgc ccacgttgct gtagcggttt
    10681 ttttcaatgg caaaaatcaa accataaaaa atcgcaaaga aagcgatgat gcccacccaa
    10741 ccaccgccca gcatggccat ggggttgatg ttagaaccga caaatgcgcc gatgccacat
    10801 gggatgaaag aaaaagccaa caacaaatag gctttgcgca aaacttgatt ttggacactg
    10861 acgcccgtat ggccatgggc aagtggttgt tcagacatgg tcatttcctt gaattagatg
    10921 aatggtcact gaaaaatgaa tcaatatagt ttgccatcat aacacacgcg gcagtggata
    10981 ctctaaaaat gccgcttggc tgcggcaaag ccagcccggt gcttattttg agcttaatgg
    11041 caaatagtca ccattgacgg tgccattcag gccgcaataa ttggttccgt tgctctgagg
    11101 ggtgctgaca cgcaagatag aacccacacg ttcaatgtgc aactgttgtt gcgggtcatc
    11161 attgctgtgg aacaccaagt gcttagcttg ggcatcggtg gcttgggcgg tgccttggaa
    11221 atcacatacc caattgccgc gggtgggctc tacgcgattc aaatgcaggc tgagggtgtt
    11281 ggcatcgcgc ttgttgatgt ccacaccccc tgaggtgaca aaccaagagc ccatgatatt
    11341 cgatgcttgt tggcggggtt taatgtgttg taaagaggat aagtgctggc gcatgagttg
    11401 gcccagagag gatttggagg cttgattcga ggatttaatt tcttctgcta ggcggtcacg
    11461 tgcgtataag aaggtgcgtt gttcctccaa taaagccgct ttaccctcgc catccaagcg
    11521 ctggttcacg tttttgtagg acagggctaa gtcgcggtca agtcggctga gtttggcatc
    11581 ttggcaaatc aagctttctg cagcagagag attttttgtg caattgaatg aaggcgcttc
    11641 cgccacggca agtctgggta tgcccatgca caggcttaaa gtgctcagaa cagtggtaat
    11701 ggtgccaaat ggtttggaac gtgccatggg gaactcgctt tcaaatgaat aagaaaaaga
    11761 attaaggcgt ttacaggccg cttatttgga catgaatcag tgtttgcctg cccgccacag
    11821 cgaccacagc atccacatgc tgttgaaaaa ggcaatgacg tagccgacaa agccaaagaa
    11881 gggcaaacca aagattttag gacctgcatc aacactcatg acgatggaag agccgataat
    11941 caatgaggct gtgatgatgc ccacggtcaa acggttggtg gatttgtcga tttgatagcc
    12001 aaacaagtcc aagcgctcca tgtcgacatt gacctggatt ttccctgcct tgagtttgcg
    12061 attgagccgt atcaaggtgt gaggcatatc gcccagcaat tgcaacaagc tgtaggtgtg
    12121 ggttttgacc ttacgcgtca tgtggtcggg tgagaagtgc tgtttcacca aaccttcaac
    12181 caagggtttg gttgattcca tcaattggaa atcgccgtcc aattgtttga ccacgccctc
    12241 taaggtaatc aaggtgcgga acagcatcac caagtctcca gggagggtga ggttgttttc
    12301 gcgtatgatg ctggtgatgt cgttgacgat ggacgaaagg ctgatgtctt ttaaggcggt
    12361 gaactcataa ttcatcagca tttccatcac atcgttgccc aagcggtctt catcggnnnn
    12421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gttaattaat gtaataattt
    12481 ctcgacgccg attgttgcct aaaaagcccg ctaatccgaa atcgatgagg gttatgcgac
    12541 cctcttcaga gatgaaaatg ttgccggggt gggggtctgc gtggaagtag ccatgtttca
    12601 gtatcatgtc caagattaac tgggcaaggt cactggcgcg gcggctcagc cattcatgct
    12661 tgtattgact tagattaagg tttttaagta aggtatcgcc gatgtattcc tgtaccaata
    12721 ttttgcgatt ggaataatca ggatagaccg ctggaatgtg cacatggtgt tgcccttcat
    12781 aggctcgagc gaaacgctcc atattgcgca gctccagcgc caaatcggtt tcgcgcgcga
    12841 ggcttttggc aaagtaatgc accatctcaa cggggtggta acggcgagac tcagcaatct
    12901 catactcgat gagttgcgcc aaatgcttca tgatgcgcaa atcggcctgt atggttttgt
    12961 cgatgttggg tcgtttgatt ttcacagcga ccacagtccc atcgagcaat atggctttgt
    13021 ggacttgggc aatagaggca ctgccgatgg gtgtgggctc tatgctgcgg aatacctcgt
    13081 ttacaggccg ctctaaatag gcactgatta acaccgaaat atcggcttgc gcaatcggcc
    13141 gcacattgtt ttgtaagcgt tggaattctt ctatccactc agggccaaaa atgtcgacac
    13201 gggtggagag gatttgacca aacttaatga aggtaggccc cagctcttca aacgccatgc
    13261 gaaagcgctg agggacactt aaatagggat tgttggcaat gtcgttatcg gcatcgcctt
    13321 tgttcagaaa actgaaggca ttggacagct tgagtctttg gatgaaatcg cccaagccat
    13381 agcgcattaa aatgccgatg atttctttca tgcgaggcaa gtcacgcatg gcaacaacgg
    13441 tttgtttcaa cataaaacag ccttaaaggt gcgcaatagc tttaacttag catagcgtga
    13501 acaggcggca caagttaaga acttggaatc gcacactgca aagagttttg atacatgatg
    13561 agtaagacga tacacagatt gttggttttg gtattaagtt gcatcagctt ggtacccata
    13621 gccacggctg accctttgga agacttaatc cgccaacgcg gcggtagtag caacagcagc
    13681 caagcgcaat cacaggcttc taaagagcaa gcaggcgact taatcatgaa tgcgatgggc
    13741 ttaatcggcc tgtcttaccg ctatggcggc aacaatccca atcaaggttt ggattgcagc
    13801 gcattcatgc aatacatttt caaacaaagc atgcgcgtaa gtttgccgcg cacagcgtct
    13861 gagcaatttg gcgtgggcag ctcggtagac cgcagcagct tacagccagg cgacatggtg
    13921 tttttccgca ccgcaggtcc tagccgcatt tcacacgtgg gcatgtacat tggtggcgac
    13981 cgctttatcc atgcgccgcg cacaggtaaa aacatcgaaa tcaccagcat caccagcaaa
    14041 tactggagca agacgtatgc aggcgcacgt cgtgtcagtg gtgcgggcaa agccgcttcg
    14101 tttgtgcgct aggggttgat gatgtctgaa atgcctccta tgcccaaatg gtttgatgcc
    14161 gagggcgagc ccgtttcctg taccgaaaaa atcaaagtca tgcagcaaaa tctgcaagaa
    14221 ttgcacgaga tggcgcaaga ggctttggaa gacgcggtat tgatgggttg caccgaagcg
    14281 caggtcaaag actatttgcg cgtgttg
//
LOCUS       DAOMVK010000007        13201 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_1063_length_13201_cov_3.023962, whole genome shotgun sequence.
ACCESSION   DAOMVK010000007 DAOMVK010000000
VERSION     DAOMVK010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 13201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13201
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1063_length_13201_cov_3.023962"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..146
                     /locus_tag="PK856_00630"
     CDS             <1..146
                     /locus_tag="PK856_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRL00906.1"
                     /translation="CNQVTEFFDPRIEEIQEQIATERGYKIVDHSLYMYGVCADCQAK
                     AKK"
     gene            150..881
                     /gene="aat"
                     /locus_tag="PK856_00635"
     CDS             150..881
                     /gene="aat"
                     /locus_tag="PK856_00635"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HRL00907.1"
                     /translation="MALQWLGDDVTDWPDLAEAQRERDGLVAVGGDLSVARLLAAYQR
                     GIFPWYSEDQPICWWALSPRMVLKPENLHVGRSLAKNLRNRHYAISVNQQFSAVINAC
                     ARMPRPGQDGTWLTQELQWALKQMYRAGHAHSFEYWQVSEEDASSLQLLGGLYGIQIG
                     QVFFGESMFALTGDASKIAFVHAVKYLQSCGIALIDCQMNTPHLARFGAEEIHFDEFQ
                     RALNLHCQQDLSEMVSGKLLVNQIN"
     gene            890..1711
                     /gene="dapD"
                     /locus_tag="PK856_00640"
     CDS             890..1711
                     /gene="dapD"
                     /locus_tag="PK856_00640"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HRL00908.1"
                     /translation="MSTLQTIIEQAFENRAEITPAAAPADVKEAVNQVIAQLNSGELR
                     CAEKQNGAWVVNEWVKKAVLLSFRLNDNIVLNDGVSEYFDKVPTRFEGYSEQDFRNGG
                     FRVVPGAVARTGSFIGKNVVLMPSFVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQASPTIIEDNCFIGARSEVVEGVIVGEGSVISMGVFIGQSTKILNRE
                     TGEVTYGRIPAGSVVVSGSMPSKDGSHSLYCAVIVKKVDAQTRSKTSVNDLLRGI"
     gene            complement(1847..2287)
                     /locus_tag="PK856_00645"
     CDS             complement(1847..2287)
                     /locus_tag="PK856_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00909.1"
                     /translation="MHNLYELLGISPYANRQEIETALQQQQRLGNVPTEMLTLAATTL
                     LSSSERQNHDQQLMAMALADFQAPENTSLLTQQNQALSDHHQPNLADSPVNSGDGDIV
                     FYDGLHHQPRPFHSNDSSRADTIVSAVFDADTVLSTLGDVLDFS"
     gene            complement(2604..3305)
                     /locus_tag="PK856_00650"
     CDS             complement(2604..3305)
                     /locus_tag="PK856_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00910.1"
                     /translation="MLNYYHLLCIHPTASRADIEIALAKQHALGKLPPATLKLAADTL
                     LTPASREVYDAKLTSLPEMNQAYALCADDLALKETVSTPHAARETANGNIPEETDNSI
                     TLYDNIRLPETNSRAFSMAKVKHTIMTSPVIWLLFAILMFKLFGMANQGTDFDVAEER
                     CIAAIQKQLIYPNSLKITPTEPKEHAWRHRQTEFPVSVHYTAEPMANTTQAYRADCVY
                     DAQNKSLSKIDIIHL"
     gene            complement(3445..4221)
                     /locus_tag="PK856_00655"
     CDS             complement(3445..4221)
                     /locus_tag="PK856_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HRL00911.1"
                     /translation="MRISFIGAGRLASQLAIALAKCHTIQHIYSRDLSKAQRLAWQVK
                     AVATNAYTDLQAACDLIIIAVSDRAIADVIAAVHAHAPEALIVHTSGSTAMQVLTDIH
                     PRAGTFYPLQTFSFERSTDWTQTPLLLEAANEADLQVLQSVASELSPRVYHYDSAQRL
                     SLHLAAVFACNFSNYCYDMAKQILDAQHADFALLYPLMLETAQKSTQAEPKTMQTGPA
                     MRGDTAIIDMHQAMLQHSEREDLLKVYQLFSEQIGKRHPV"
     gene            4370..5446
                     /locus_tag="PK856_00660"
     CDS             4370..5446
                     /locus_tag="PK856_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HRL00912.1"
                     /translation="MWVGQILTNAGALPKLSHTEDWQESEKEMKNWLQKMKIDRFLML
                     LIGVIVLASVLPVQGEVKVGFEYLTTAAIALLFFMHGAKLSREAIKQGMGHWRLHLLV
                     FASTFVLFPLLGLALHFMVPSWMSMTVYMGFLYMCALPATVQSAIAFTSVAGGNIAAA
                     VCSASASSILGVFLSPILVGWLMSTDGNGEALDTAGAIGSILLQLMLPFVLGHLARPW
                     IAEWITRHRKLVGMTDQSSILLVVYVAFSEAVVEGIWHKVDAHSLMMIGVISCVLLAV
                     VITVNTYAARLLRFNKEDEITIVFCGSKKSMANGVPMANVLFPASVVGVMLLPLMIFH
                     QIQLMVCAVLAQRYARRLTENETV"
     gene            complement(5576..6403)
                     /locus_tag="PK856_00665"
     CDS             complement(5576..6403)
                     /locus_tag="PK856_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00913.1"
                     /translation="MNRNQINFIIGAVCVFVLLAFALAFFYISSQKDDSVKQVQSASD
                     ASGPEIMTLNSNASAPTAASEPEDNNTLSQLFTAKTIGSSKKMVEDITGKAISNEYGL
                     SEYEVNGCRVHATYVDDKLRNLGLDLSDSCKVNLSTFNVPNTEAGPNLTFGQISEALG
                     ASNIRQFQSDCLMNCGNAQEPTVGAVYAIDSNPNEPEPMEILIKTAQVGDAALTASDK
                     WRGAMLKQEDEDWVLNGEFNCDARYNSVAQELFNNVSVNSVTIGYDIPLESCQSAAG"
     gene            complement(6707..7468)
                     /locus_tag="PK856_00670"
     CDS             complement(6707..7468)
                     /locus_tag="PK856_00670"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRL00914.1"
                     /translation="MNVKLTATLLCSTLFLGACANNMSTNDLLSLGTSGVQALTLTDG
                     QLRDTANQSCAQMDKANNVAAANSKYSQRLNNIKKGLPSSIDGVPLNFQVYQVRDINA
                     WAMPNGCIRVYSGLMDLMTDDEVRGVLGHEIGHVQLGHSKNRFRVAALASLGREVLAK
                     SGNSTLTNLNSSELGNLSEKFLNAQFSQKQESDADDYSYTLLTSKGLSPKPLATSFEK
                     LSKLSGGKGSVFDSHPGSDKRAQRIYERMAKEGKK"
     gene            complement(7737..8744)
                     /locus_tag="PK856_00675"
     CDS             complement(7737..8744)
                     /locus_tag="PK856_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HRL00915.1"
                     /translation="MQFGKKIDTVEVHTGGEAFRIVTSGLPKFPGDTIVARRQWIKDH
                     IDHIRTALIFEPRGHADMYAGYLTEAITPTADFGVIFVHNEGYSDHCGHGVIALSTAA
                     VELGWVEPTQPETRVGIDAPCGFIEAFVEWDGQRTGKVRFVNVPSFIYQRDVSVETPS
                     FGTVTGDIAFGGAFYFYTDGRAHGLEINEANVARLIQFGDEVKQAANKAYPVVHPFIP
                     ELNHIYGTIVDGNPRHAGSSQANCCIFADRQVDRSPTGSGTAGRVAQQYLRGLLAKDE
                     VLANESIVGSVFTGKVLSETQVADFAAVIPEVTGDAHVYGFASWLMNEHDPLDAGFLV
                     R"
     gene            complement(9085..9984)
                     /locus_tag="PK856_00680"
     CDS             complement(9085..9984)
                     /locus_tag="PK856_00680"
                     /inference="COORDINATES: protein motif:HMM:NF014708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRL00916.1"
                     /translation="MAKLEITVLKSTELSPGFQIQRALPQRGLRRVGAWAFLDHIGPV
                     QLSPTLGMHVHSHPHIGLSTFTWMISGEIQHHDSLGHNLAIKPGQVNLMTAGNGIAHT
                     EVSIGESGCMHAAQLWIALPEHIKDIPASFQNYPEMPRIASAHLTETVLIGQWQDCIS
                     PVTAHTPIVGVDLEAHADYEQVFELNPAFEYGFLPMIGDLTVNGETVKTGEFAYLPTG
                     ESSLSLQANAGSRALLIGGEPLDEELILWWNLIARTQAEIEQARADWEAQTERYGELT
                     QLGDGWLHAPEITGNLIASKGHR"
     gene            10134..>10564
                     /locus_tag="PK856_00685"
     CDS             10134..>10564
                     /locus_tag="PK856_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003762218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HRL00917.1"
                     /translation="MINTHPEAVLQFWFERLTPEQRFSKDDAIDASIRREFFHTWQAA
                     ARGELSAWRERMSGRLAEIIVLDQFSRNLWRGDALSFAQDGMALVLSQEAQGQTGFAA
                     LTSVEHQFLLMPMMHSESLAIQDASLPLFNRFCDDNTYGFAV"
     assembly_gap    10565..10574
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10575..10718
                     /locus_tag="PK856_00690"
     CDS             <10575..10718
                     /locus_tag="PK856_00690"
                     /inference="COORDINATES: protein motif:HMM:NF017819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HRL00918.1"
                     /translation="DANTLDFAQRHRDIVAEFGRFPHRNAVLKRDSTMAEQAFLQQPN
                     SSF"
     gene            10746..11270
                     /locus_tag="PK856_00695"
     CDS             10746..11270
                     /locus_tag="PK856_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00919.1"
                     /translation="MRYFHYMDTESQDQPISVYVALDDEAWPLQALFGASVAEEVALQ
                     WHNSSLNVNHPRHGLPEACMQEFVSEMQEVSAEVFHSYWRLSLEKQWPQWLTLQQNLA
                     LGQSLTAKIVCFYPQGVIADVGLDFYAWLDYDECLAWVGKENMRPQHEMVACVSGFDN
                     ENLWIKLKHERAST"
     gene            11251..11730
                     /locus_tag="PK856_00700"
     CDS             11251..11730
                     /locus_tag="PK856_00700"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRL00920.1"
                     /translation="MNVHPHEVTLVSVRERPECAAAMIAFFQQSWPEILPEMYADSIH
                     HSIDAPQALPQWYVLQKDGEIVACAGLITNDFISRGDLYPWLCALFVNEAERRQGYAR
                     MLIERCRADAIKAGFAKLYLCTDTVNLYERYGFEYLAQGWHPWGETSRIYQYTLTTD"
     gene            12195..>13201
                     /locus_tag="PK856_00705"
     CDS             12195..>13201
                     /locus_tag="PK856_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRL00921.1"
                     /translation="MQLSGAQIIVQSLIAEGVEYVFGYPGGAVLEIYDAIYQQEQFRH
                     ILVRHEQAAIHAADAFARSRGTGKAGVALVTSGPGATNAITGIATAYTDSIPLVVISG
                     QVGTPAIGSDAFQEVDMVGVSRPCVKHNFLVTDIADLAETIKKAFYIAESGRPGPVVI
                     DIPKDITQAMAKFSYPEEVFIRSYQPATKGHTGQIKKALQVLAGAKRPLIYFGGGVVL
                     GDAAGELTELVKLLGAPTTGTLMGLGAYDSTDRQFIGMLGMHGFYEANLAMQEADVVV
                     AVGARFDDRVISVPSKFLATPKKIIHIDVDPSSIAKRVKVNVPIVGDVKLVLQEMIAL
                     W"
BASE COUNT         3124 a         2857 c         3641 g         3569 t
ORIGIN      
        1 aatgcaatca agtcaccgag tttttcgatc cacgcatcga agaaatccaa gaacaaattg
       61 ccaccgagcg cggctataaa atcgtcgacc actccttgta catgtacggt gtgtgcgctg
      121 attgccaagc caaagccaaa aaataaacca tggccttgca atggttgggt gatgatgtta
      181 ccgattggcc agaccttgct gaggcccaac gtgagcgcga tggcttggtt gccgtaggtg
      241 gcgatttgag tgtagcacgc ctgcttgccg cgtaccaacg cggcattttt ccgtggtaca
      301 gcgaagacca acccatatgt tggtgggctt tatcaccgcg catggtcctc aagccagaaa
      361 acctgcatgt cggccgctct ttggcaaaaa acctgcgcaa tcgccactat gccatcagcg
      421 tgaatcagca attcagcgcc gtcatcaatg cttgcgcccg catgccaagg cctggacaag
      481 atggcacttg gctcacgcaa gaattgcaat gggctttaaa acaaatgtac cgcgcaggcc
      541 atgcccacag ttttgagtat tggcaggtct cggaagaaga cgccagcagt ttgcaattgc
      601 ttggcggcct gtatggcatc caaattggtc aagtgttttt tggcgaatcc atgtttgcct
      661 taacgggcga cgcctcaaaa atcgctttcg tccacgcggt gaaatactta caatcatgcg
      721 gcatcgcatt gatcgattgc caaatgaaca cgcctcattt ggcacgtttt ggcgctgaag
      781 aaatccactt cgatgagttt cagcgcgctt tgaatctgca ttgccaacag gatttgtcgg
      841 aaatggtttc tggcaaattg ttggtgaatc aaatcaacta gggagcatca tgtctacatt
      901 acaaaccatc attgaacaag cttttgaaaa ccgtgctgaa atcaccccag cggctgcgcc
      961 tgcggatgtg aaagaagccg tcaaccaagt catcgcccaa ttgaacagcg gtgaattgcg
     1021 ttgtgctgaa aagcaaaacg gtgcttgggt ggtgaatgag tgggtgaaaa aagcggtttt
     1081 gttgtctttc cgtttgaacg acaacatcgt gttaaacgac ggcgtgagcg agtatttcga
     1141 taaagtgcct acccgtttcg aaggctatag cgaacaagat ttccgcaatg gcggcttccg
     1201 cgtagtacct ggtgctgtag cgcgcacagg ctctttcatc ggtaaaaacg tggtattgat
     1261 gccttcattc gtgaacatcg gcgcttacgt agacgaaggc acgatggtag acacttgggc
     1321 cactgtgggc tcttgcgcgc aaatcggtaa aaacgtgcat ttgtcaggcg gagtgggcat
     1381 tggcggcgtg ttagagccat tgcaagccag cccaaccatc attgaagaca attgcttcat
     1441 cggcgcgcgt tctgaagtgg tagaaggcgt gattgttggc gaaggctctg tgatttctat
     1501 gggcgtgttc atcggtcaat ccaccaaaat tctcaaccgt gaaaccggcg aagtgactta
     1561 cggccgcatt ccagcaggtt cagtggtcgt gtctggttct atgcccagca aagacggcag
     1621 ccacagcctg tattgcgcgg tgattgtgaa aaaagtcgat gcgcaaaccc gcagcaaaac
     1681 cagcgttaac gatttgttgc gcggtattta agtgttgatt tgaacgctgt cagcgttcaa
     1741 atatattgat aaaagcggcc tgtaaagttt acaggccgct tttgtgtgtg ctcgagttgt
     1801 taagtaatgc acttgaatgg gtttaatcac actgaagcac tgctgcttaa gaaaaatcta
     1861 agacatcgcc caaagtgctg aggacggtgt cggcatcgaa aacggcgctc acaatcgtgt
     1921 ctgcacgcga ggagtcattg gagtggaatg ggcgaggttg atggtgtaag ccatcataga
     1981 aaacaatgtc gccatcacca gagttaacag gcgagtctgc caaattgggc tgatgatggt
     2041 cggacaatgc ttgattctgt tgtgttaaaa ggctggtatt ttctggggct tggaaatccg
     2101 ctaaagccat tgccatgagt tgttggtcat ggttttggcg ctctgaggaa gaaagcaatg
     2161 tggtggccgc aagcgtcagc atttctgtgg gtacatttcc caaacgctgt tgctgctgca
     2221 aggctgtttc tatctcttgg cggttggcat aaggggaaat gcctaagagt tcgtataaat
     2281 tgtgcatgac caagctcgct ttataaggca attcaaacca taaaatgatt gaaaaacggt
     2341 cggatacgct gaggatgcgt tgtgtccagt ttgttttatg gtagcagatt gttgaaatca
     2401 atgcttaaga acaattttga attcatagag ggcagactgt gttgttctta ttgtgaatct
     2461 ctttgtttta agcggcctgt aaacaggttg aaaccacgat atgtacaggc ttaggatagc
     2521 atgatgatgc tatgcataga gatggttgat gtatttcatc aatggttttt gggtttttgc
     2581 aaacagcgcc atcacacatc cagtcacaaa tgaatgatgt ctatcttact caaacttttg
     2641 ttttgggcat cgtaaacaca gtcggctcgg taagcttggg tggtgttggc catgggttct
     2701 gcggtgtaat ggacgctgac aggaaactcg gtttgacggt ggcgccatgc gtgctcttta
     2761 ggctcagtcg gggtgatttt caagctgttg ggataaatca gttgtttttg gatggcagcg
     2821 atgcaacgct cttcggccac atcaaaatcc gtgccttggt tggccatgcc gaataatttg
     2881 aacattaaaa tggcaaacaa tagccaaatg acgggagagg tcatgatggt gtgtttgact
     2941 ttggccatgg aaaaagccct gctgttggtt tcaggcaggc ggatgttgtc gtatagggtg
     3001 atgctgttgt ctgtctcttc aggtatgttg ccgttggcag tttcgcgtgc agcatgtggg
     3061 gtgctgacgg tttcttttaa ggccaaatcg tctgcacata aggcataggc ttggttcatt
     3121 tcgggcaggc tggtgagctt ggcgtcatac acttcgcggc tggcgggtgt gagtaaggta
     3181 tcggctgcga gcttgagggt ggccggtggt aatttgccca aggcgtgttg cttggccaaa
     3241 gcaatttcaa tatcggcacg gctggcggta gggtgtatgc acagtaagtg gtagtaattg
     3301 agcataaagt ctcctgtgac gtgggcgcga cgcattgcaa ttgaaaggga tggcagaaga
     3361 tattattgtt ttgtgatgca tgattgtatc aagaaatgcg gcctgtaaac gcttacaggc
     3421 cgcatttcat ttgcatgttg atgattatac tgggtggcgt ttgcctattt gttcactgaa
     3481 gagttggtag actttgagca aatcctcgcg ttcgctgtgt tgcaacatgg cttggtgcat
     3541 gtcaataatg gcggtgtcac cacgcatggc ggggcctgtt tgcatggttt tgggctcggc
     3601 ttgcgtggat ttttgggcgg tttccaacat caaggggtag agcagggcaa agtcggcatg
     3661 ttgtgcgtcg agtatttgct tggccatgtc gtagcaataa ttgctgaaat tgcaggcaaa
     3721 caccgctgcc aaatgcaaac tcaaacgctg ggcggaatca tagtggtaaa cgcgcggact
     3781 caattcgctt gccacacttt gcaagacttg caagtcagcc tcgttggctg cctcaagcaa
     3841 cagcggcgtt tgcgtccaat cggtgctgcg ttcaaagctg aaggtttgca aggggtaaaa
     3901 cgtgcctgca cgcggatgga tgtctgttaa cacttgcatg gcggtgctgc ctgaggtgtg
     3961 cacaatcaaa gcttcgggtg catgggcatg cacggcggcg atgacgtcgg caatggcgcg
     4021 gtcgctcaca gcgatgatga tgaggtcgca agcggcctgt aaatcggtat atgcattggt
     4081 ggcaacggct ttaacttgcc atgccaagcg ctgtgctttg cttaaatcgc ggctgtaaat
     4141 gtgctgtatg gtgtggcatt tggccaaggc aatagccaat tggctggcga ggcggccagc
     4201 gcctatgaaa ctgatgcgca tgaggaaaag tggtcggatg aaacattcag catgcctatg
     4261 ccaaactgaa attgcaagtc aggatgcagg cttgcttgag agatggctgg catattgcca
     4321 aaattcctga atttttttgg tggttttgtg tgcatttctt gaataaatcg tgtgggtggg
     4381 ccaaattttg acaaacgcag gcgcactgcc taagctgagt cacactgaag actggcaaga
     4441 gagcgagaag gaaatgaaaa actggctgca aaaaatgaag atagaccgtt ttttaatgct
     4501 gctcatcggc gtgatagtgc tcgccagcgt gttgcctgtg caaggcgaag tgaaagtggg
     4561 ctttgagtat ttgaccacgg cggcgatagc cttgctgttt ttcatgcatg gggcgaagtt
     4621 gtcgcgcgag gcgattaagc agggtatggg acactggcgt ttgcatttgt tggtgtttgc
     4681 cagcacgttt gtgttgtttc cgctgctggg cttggcgctg cactttatgg tgccctcgtg
     4741 gatgtcgatg acggtgtaca tgggcttttt gtatatgtgt gcattgcctg caaccgttca
     4801 atctgcgatt gccttcacct cggtggcggg cggcaacatc gcggcggcgg tgtgcagcgc
     4861 atcggcttca agcattttgg gggtgttttt gtcgccgatt ttggtgggtt ggctgatgag
     4921 taccgatggt aatggcgagg ctttggacac ggcaggcgcg atagggtcga ttttgttgca
     4981 attgatgttg ccatttgtgc ttgggcattt ggcgcgccct tggatcgccg agtggattac
     5041 ccgccaccgc aaactggtgg gcatgaccga tcaatcgtcg atattgttgg tagtgtatgt
     5101 ggcattcagc gaggcggtgg tcgaaggcat ttggcacaaa gtcgatgccc attcactcat
     5161 gatgataggg gtgatttctt gtgtgttgtt ggcggtggtg attaccgtca atacctatgc
     5221 ggcgcgtttg ttgcgtttta acaaagaaga cgaaatcacc attgtgtttt gtggctcgaa
     5281 aaaaagcatg gccaatggtg tgccgatggc gaatgtgttg tttcctgctt cagtggtggg
     5341 ggtgatgtta ttgcccttga tgatttttca ccaaatccaa ttgatggtgt gcgcggtgtt
     5401 ggcgcagcgc tatgcacggc gtttgactga aaatgaaacg gtttaagtga tggtgtgcat
     5461 gagctgagcc agacggattt tggtggatac agttagcaag caaataagcg gcctgtaagg
     5521 tggaaactta caggccgctt taatgatggt gacaggcaca aacagaaggg ttaaatcagc
     5581 ctgctgccga ttgacaagat tctaggggaa tgtcgtagcc tatggtgacg gagttcacgc
     5641 tgacattgtt gaacaactct tgggcaacgc tgttgtaacg ggcatcgcag ttaaactcac
     5701 cgttcaacac ccagtcttcg tcttcttgct tgagcatggc gcctcgccat ttatcactgg
     5761 cggtaagggc cgcgtcaccc acttgtgctg ttttgattag tatttccatg ggctcaggct
     5821 cgttggggtt ggaatctatc gcataaacag cgccgactgt gggctcttgg gcattgccac
     5881 aattcatcag gcagtcggat tgaaattggc ggatgttgct cgcgcccaag gcctcactga
     5941 tttgtccaaa ggtgagattg gggccagctt cggtattggg aacattaaaa gtcgacaaat
     6001 tgactttgca gctgtcgctc aaatccaagc caaggtttct gagtttgtca tccacatacg
     6061 tcgcatgcac gcgacagccg tttacctcgt attcgctcaa gccgtattca ttgctgatgg
     6121 ctttgcctgt gatgtcttcc accatttttt tgctgctgcc tatggttttg gcggtaaaca
     6181 attggctgag ggtgttgttg tcttctggtt cactggccgc ggttggggct gaggcattgc
     6241 tgttcaaggt catgatttcg gggccagaag catcgctggc gctttgcact tgtttgacgc
     6301 tgtcgtcttt ttggctgctg atgtagaaaa aagccagcgc aaacgccaac aagacaaaca
     6361 cacacactgc tccgatgatg aagttgattt ggttgcggtt catgggtttt ccttatgcat
     6421 ggatgacgcg atattgtgta taccatttgg atgcggtttt ggcatcataa aataacatgc
     6481 aatggtgtgc agtggtttgg cgtcgggcag caaaaaattg cacgccaagg ggcaaatgtt
     6541 ttgtgtggcg gaagttggaa atgtcatggc cagtgagctg agctgtgtga tatcggtttt
     6601 cgattgggca cacgagtggc aatgctaagc tgcaatcaat acggcctgta agtgtttaca
     6661 ggccgcttgt catgcaatat tttgtgctca gaacacaaag tcgtgcttat tttttgcctt
     6721 ctttggccat gcgttcgtag atgcgctgcg cgcgtttgtc tgagcctggg tggctgtcaa
     6781 acactgagcc tttaccacct gacagtttgg acagtttttc aaaactggtg gccaatggtt
     6841 tcgggctcaa acctttgctg gttaacaagg tataagaata atcatcggca tcggattctt
     6901 gtttttgcga gaattgggca ttcaggaatt tttctgacag attacccaat tcagacgaat
     6961 tcaaattggt aagggtgctg ttgccgctct tggccaaaac ctcacggcct aaagacgcca
     7021 aggctgccac gcggaagcgg tttttgctgt ggcccaattg cacatggccg atttcatggc
     7081 ccaaaacacc gcgtacttcg tcatccgtca tcaaatccat caaacctgaa taaacacgga
     7141 tacagccatt gggcatggcc caagcattga tgtcacgcac ttggtaaacc tggaaattca
     7201 aaggcacacc atcgatgctg ctgggcaagc cttttttgat gttgttcaag cgttggctgt
     7261 atttgctgtt cgcagcagcg acattgttgg ccttgtccat ttgggcacaa gattggttgg
     7321 ccgtgtcgcg caattgtcca tcggttaagg tcaaggcttg cacacctgat gtgcccaaac
     7381 ttaataaatc gttggtactc atgttgttgg cacaagcgcc caaaaacaaa gtggaacaca
     7441 atagggttgc ggtcagtttt acattcatca tcattccttg taggcgggtt gattattgtc
     7501 agagcaaaaa tgtctatatg gctgaacaga gcttgatgtt aacactcaat gcgatggtgt
     7561 tggtgcatgc aatttacgtg cccgcattct gttggcatgt tttcataggg cggggcgaca
     7621 ttatttgtgc aggtgctgag aacatggcaa acagcaaaat ccgttttgta tgattcatga
     7681 acatgcggcc tgtaagttta caggccgcat gttcattgtt tgattaagta tggcttttaa
     7741 cgtaccaaga aacctgcatc aagcgggtcg tgttcgttca tcaaccagct ggcgaaacca
     7801 tacacatgcg cgtcgcccgt gacctcgggg atgacggcgg caaagtcggc cacttgggtt
     7861 tctgacaaaa ccttacccgt gaacaccgag ccaacgatgg attcgtttgc caacacttca
     7921 tctttggcga gcaaaccgcg caagtattgc tgcgccacac ggcctgccgt gcctgagcct
     7981 gtaggcgagc ggtcgacttg gcggtcggcg aaaatacagc aattggcttg actggagcca
     8041 gcatggcgag gattgccgtc gacgatggtg ccgtaaatgt ggttgagctc ggggatgaac
     8101 gggtgaacca cagggtaggc tttgttggcg gcttgtttca cttcgtcgcc aaattggatt
     8161 aaacgggcga cattggcctc attgatttcc aaaccgtggg cgcgaccgtc ggtgtaaaag
     8221 taaaacgcgc ccccaaaagc gatgtcgcct gtgaccgtgc caaagctggg tgtttccacg
     8281 ctgacgtcgc gttggtaaat gaacgagggc acgttgacaa agcgcacctt gcctgtgcgt
     8341 tggccatccc attcgacaaa ggcttcgatg aagccgcaag gtgcatctat gcccacgcgg
     8401 gtttcgggtt gggtgggttc tacccagccc aattccactg ctgcagtcga taaggcaatc
     8461 acaccgtggc cgcagtggtc gctatagcct tcgttgtgca cgaaaatcac gccaaagtcg
     8521 gcagtcggtg taattgcttc ggtcaaatag cctgcataca tgtcggcatg gccgcgtggc
     8581 tcaaaaatca aggcggtgcg gatgtggtcg atgtggtctt taatccactg acggcgcgcg
     8641 acaatggtgt cgcctggaaa tttgggcaag cccgatgtca cgatgcgaaa ggcttcgccg
     8701 cctgtgtgca cttctacggt gtcaattttt ttaccgaatt gcatggatgc tctctttaat
     8761 agacaaatat tatggtgttg tgtgaggcgg cttttacagg ccgcttaaac cttgtgcaga
     8821 ctatcagttt cgctgagctt gttcaattct caaactttgt taaaacgaaa ttggtgaggg
     8881 cgtagggcga gaatgtggtg ttcatcaaaa gaagcatggc ttagatgggg aaaacaacaa
     8941 gttgtggatg tcgattgtgc gtaagtttgt agtggatgat tcgttcaaag ctacagttgt
     9001 ttgtaaacca gtaaaaagtg gcctgtaaat gtttacaggc cgcttgttaa aagatagcaa
     9061 ccagaatgcc gctgctattg gtgcttatct gtgacccttg ctggcaatca aattgccagt
     9121 gatttcgggt gcatgcagcc agccatcgcc caattgggtt aattcgccat agcgttcggt
     9181 ttgagcttcc cagtcggcgc gggcttgctc gatttctgct tgggtgcggg caatcaaatt
     9241 ccaccacaaa atcaactctt catccaaagg ctcgccgccg attaataagg cgcggctgcc
     9301 tgcgtttgct tgcaagctca ggctggattc acctgtaggc aagtaggcaa attcgcctgt
     9361 tttgacggtt tcaccattga ctgtcaaatc gcctatcatc ggtaaaaagc cgtactcaaa
     9421 agcgggattc aactcaaaga cttgctcata atcggcatgt gcctccaaat ccacgcccac
     9481 gatgggagtg tgcgcagtca caggggagat gcaatcttgc cactggccga ttaatacggt
     9541 ttcggtaagg tgggcagaag caatgcgggg catttctggg tagttttgga atgaggcagg
     9601 gatatctttg atatgctcag gcaaagcaat ccacaattga gccgcgtgca tgcagcccga
     9661 ttcgcctatg ctgacttcgg tgtgggcgat gccattgcct gcggtcatca aattcacttg
     9721 gccaggttta atcgccaaat tgtggcccaa gctgtcatgg tgttggattt cgccgctgat
     9781 catccatgtg aaggtcgaca aaccgatgtg tggatgcgaa tgcacatgca tgcccagagt
     9841 tgggctcaat tgtactgggc cgatatggtc taaaaatgcc caagcgccaa cgcggcgcaa
     9901 accgcgctgc ggtaaggcgc gttgtatttg aaagccagga cttaattctg tgctttttaa
     9961 aaccgtgatt tcaagttttg ccatgtgatc attcccagtt tgaatagtgt tgaaagctta
    10021 acacagcctg atggcaggtg gcttgtgcta tgctgagtat ttgctaatga agtgaaacac
    10081 gctggttttg aatgaattaa tgtgtcaata cgcagttgaa aaggaagcct ttaatgataa
    10141 atacccatcc tgaagcggtt ttgcaatttt ggtttgagcg cctaacgcct gagcagcgat
    10201 ttagcaaaga cgatgccata gacgccagca ttcgccgtga gtttttccat acttggcaag
    10261 cagcggcgcg gggcgaattg tcggcttggc gtgagcgcat gagtggtcgt ttggccgaaa
    10321 tcattgtgct agaccaattc agccgcaatt tgtggcgcgg cgatgcgctg tcttttgccc
    10381 aagacggcat ggctttggtt ttgagccaag aagcgcaggg acaaacagga tttgcggcat
    10441 tgacttccgt cgaacaccaa ttcttgttga tgccgatgat gcattcggaa tctttggcga
    10501 tacaagatgc gtctttgcct ctgttcaatc ggttttgtga tgacaacacc tatggctttg
    10561 cagtnnnnnn nnnngatgcc aacacgctgg attttgccca acgtcaccgc gacattgtgg
    10621 ctgagtttgg tcgctttccg caccgcaatg cggtgttaaa acgcgacagc actatggctg
    10681 agcaagcctt tttgcaacag cccaattctt ctttttgagc ttacaggccg cttgatagag
    10741 aagacatgcg ctatttccat tacatggaca ccgaaagcca agaccaaccc atctctgtgt
    10801 atgtggcttt ggacgacgaa gcttggcctt tgcaagcgct ctttggagca agtgttgcgg
    10861 aggaagttgc tttgcaatgg cacaacagca gcctaaacgt gaatcaccct cgccatggtt
    10921 tgccagaagc ctgtatgcaa gagtttgtgt ctgaaatgca agaagtgagc gctgaggtgt
    10981 ttcattctta ttggcggctt tcgcttgaaa agcagtggcc tcaatggctg accttgcagc
    11041 aaaatttggc gctaggccaa agtttgacgg caaaaatcgt gtgtttttac cctcaaggtg
    11101 tgattgccga tgtgggtttg gatttttatg cgtggcttga ctacgatgag tgtttggctt
    11161 gggtgggcaa agagaacatg cgaccccaac atgaaatggt ggcctgtgtg tcaggatttg
    11221 acaatgaaaa cttatggatt aaattaaaac atgaacgtgc atccacatga agtgactttg
    11281 gtttcagtga gagagcggcc tgaatgtgcg gcggctatga ttgctttttt tcaacaaagc
    11341 tggcctgaaa tactgcccga aatgtatgcc gattcaatac accacagcat tgatgcgcca
    11401 caggctttgc cccaatggta tgtgttgcaa aaagacggtg aaattgtggc ctgtgcgggc
    11461 ttgattacca atgatttcat cagtcgcggg gatttgtatc catggctgtg tgctttgttc
    11521 gtgaatgaag cagagcgcag gcaaggctat gctcgcatgt tgattgagcg ttgtcgggcc
    11581 gatgccatta aagcaggatt tgctaaattg tatttgtgta ccgatacggt gaatttgtat
    11641 gagcgctatg gtttcgagta tttggcgcaa ggctggcatc cttggggcga aacttcacgc
    11701 atttaccaat ataccctcac cacagattaa agccttgcag agcgaatcga acacggcgaa
    11761 accaatgctt acaggccgtg tttgagcaag tattttaatt ttagacaatt tgtccataaa
    11821 tttcaggtaa atcaatggat aaggcaaaac taattttggc agtaacaatt aaataaatgc
    11881 caatatagaa aaattctatc gcattaaatg ctttttaata tcgaaaaatt ggcattaaca
    11941 ttttcttggt aagtttttgt taaaaatctc aacgtctatg tcttgccaat aatgctgcat
    12001 tgcagtatct ttgactgcaa gttttcttgt tcaaacccaa aacggcagtt tcaaatgact
    12061 gtgtaaatgg attgtcatga ctagggtgtt ggcacgtacc tcaaaccgtc gtcaacattc
    12121 agtggccttg ctagcgggcc ttgtgtgaaa tcgtttatgc cttttgaagc ccccgtgtat
    12181 taaaagaggt gtttatgcaa ttatcaggtg cacaaatcat tgtgcaatcc ctcatagccg
    12241 aaggcgttga atacgttttt ggttatccag gtggcgcggt attggagatt tacgacgcca
    12301 tttatcagca agaacaattc cgtcatattt tggtaagaca cgaacaagct gcgattcatg
    12361 ctgctgatgc gtttgcccgt tctcgtggca caggcaaagc aggtgtggct ttggtgactt
    12421 cgggtcctgg tgcgaccaat gccatcacag gcattgccac tgcgtatact gactccattc
    12481 ctttagtggt gatttctggc caagtgggca cgcctgcaat tggttcagat gcgttccaag
    12541 aggtggacat ggtgggcgtg agccgtccat gcgtgaagca caacttcttg gtaaccgaca
    12601 ttgcggactt ggctgaaacc atcaaaaaag ccttttacat tgccgaatca ggccgcccag
    12661 gtcctgtggt gattgatatt ccgaaagaca ttacccaagc gatggcgaaa ttcagctatc
    12721 cagaagaagt gtttattcgc tcatatcaac ctgcaaccaa aggacacaca ggccaaatca
    12781 aaaaagcctt gcaagtattg gcaggtgcca aacgcccatt gatttatttc ggtggtggtg
    12841 tggtattggg tgatgcagcg ggtgagttga ccgaattggt gaaattgttg ggtgcgccaa
    12901 ccacgggcac tttgatgggc ttgggtgcat acgactcgac tgaccgccaa ttcattggca
    12961 tgctgggcat gcacggtttt tacgaagcca atttggccat gcaagaagcc gacgtggtgg
    13021 tggcagtggg cgcgcgtttt gacgaccgtg tgatttcggt accgagcaaa ttcttggcca
    13081 caccgaaaaa aatcattcac atcgatgtag acccatcgag cattgccaag cgcgtgaaag
    13141 tgaacgtgcc aatagtgggt gacgtgaaac tggtgttgca agagatgatt gccttgtgga
    13201 a
//
LOCUS       DAOMVK010000008        12841 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_1108_length_12841_cov_2.923979, whole genome shotgun sequence.
ACCESSION   DAOMVK010000008 DAOMVK010000000
VERSION     DAOMVK010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 12841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12841
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1108_length_12841_cov_2.923979"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PK856_00710"
     CDS             <1..214
                     /locus_tag="PK856_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00922.1"
                     /translation="GDGIEPKRRLKAIGSAFAVAEPSRLKNGCGKGLGTKGLGIVYCR
                     LNIIPHNRLIFFFVIDCHHRINLTDI"
     gene            complement(423..1337)
                     /locus_tag="PK856_00715"
     CDS             complement(423..1337)
                     /locus_tag="PK856_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00923.1"
                     /translation="MGTPVKVFRSTDTDAPQIIGGRGDFKTVLKACLAAGYGSGSNKK
                     EPLGWEVVAGTESADGFDCAFRPTDLESGRHIIHVAGTTTSTATISAYMDTDAAGALI
                     EKAPAIDVFPTFHSTGIEWILVGHAKAFIIIIGIKGAFSGKARANGAGLFFGDIENRV
                     NNPRGNTILFRISNLGHFGSGGTTSKNIQFWNGDSSLISQSVNGLSNWVKVIPSALFT
                     DNLLIGNSPINSEVIYSKIAVTDAGMLRGFLPFGYLIHQSLMPTEHFAEKSVDGKNYL
                     ICNLTRSGWATHYLAAPMFFMVNLTEWD"
     gene            complement(1348..4815)
                     /locus_tag="PK856_00720"
     CDS             complement(1348..4815)
                     /locus_tag="PK856_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00924.1"
                     /translation="MAQQTTRLTQQDLKIYPSERLTDTDDGGGLMRGTALTGADNELF
                     PPVSDVDRTMGAFNARLVYPAVLREDEEPLYGGHCIVSERAVMDNVSFLLFGAQNYGE
                     SRKDILPRIEAYSVPTIESRMTLLGRQLSGTRTIQAYQRIEAPVPIVGERYCLSFEEK
                     NKTPVQEYFRILTMTHEIRTFEDADGKEFQRRVIKMEISNPLARDFSGVDYPTGRGYA
                     SSPTKILETQVADTATYYGCRPLVEPITASTATIRVDDIFEKIVPTSLVETAYIDQYP
                     SKTAYWIETGLKQAVFTGSRNAGDLYLNQSVLPGSVELLNYKDNAQGQLIDGENVLTI
                     DYNNAVIRNVPALYNAVVQAVPAAKVSNSSCSTIINIDDTNQGTEWAPLLNPKPANGS
                     VSVAWMSQGTWYELTDHGDYNLRDKEGKVRGAVARTGSVVISLPEQPDVGSKIIITWA
                     PVDFYKTINDKDAGSVIVPVALSPELSLLQVPQPIIKPGTVKINTGTSTVTDNGQGAL
                     TGALTGRVDYAGGQIYLSAGSSIAEADIGFDYYADEYTKTSFTLETTATQISGSIGPT
                     VRGTAKIVADLYYHEDIKAWNYMGTDFVEVQERNMFVFTDNGNGQLMRNGKLVASSSI
                     DYSTGLFIVPFNSIKGDCVTPSWESTEAVLNQSSIGEWGIFKVVSRDVTPLPGPSSYF
                     YKLQSSATRVYSSKQAISYYSTNVLTGKPQPLQCVPNSFVFEVGGTKVYERMGRLYRN
                     INSTTGNGEDIGTLSAYGDLAITNPSLALGTIKVLAGVYAVGGLLGKQTYGYTLAAPV
                     KPQSFTAYANGQSGLIVGTAGANGAITGDITGTIETKTGFYELTSVKGFAPESVRYNT
                     VVQSNIPLDSSIIGIDAVRLPPDGRVPIFRRGDMIVIGNKVKQGIGSAHTGGQIIQLS
                     RDDLSRVCLVDANAKHINAELYTVDLADGTVTWEPQIDLSAYTMPITAHHVQEEENRI
                     VGLDINGTLKLQFATSRAYPVDGTTISSAFIGGNLLTRVTAPFSQQAWNKVWSNERQT
                     DEILAKLNVKDYPIKLASNGAITERWLLLFKTDAQFDVIGENLGLIATSDRLTDLAPP
                     NPATGQPYFTLPKGAFGGGWMSGNCIRFNTFSSQMPTWVLRAVQPSPVAQTGKDGFTM
                     CLRGNTVDK"
     gene            complement(4897..5301)
                     /locus_tag="PK856_00725"
     CDS             complement(4897..5301)
                     /locus_tag="PK856_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00925.1"
                     /translation="MANWKLKRNDTSEELEFDQDMHWTDEFNWSAIAQTQPVYSLAGA
                     VLVQQGIKQAGRPITLDGDWVWYGRTLVERLRAWTDVPGLKMTLIHYDGRQFTVGFRY
                     HDNSLNKVEPVRYATPEAGADRYTMAIQLMTM"
     gene            complement(5301..9029)
                     /locus_tag="PK856_00730"
     CDS             complement(5301..9029)
                     /locus_tag="PK856_00730"
                     /inference="COORDINATES: protein motif:HMM:NF040129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HRL00926.1"
                     /translation="MSQEAKRLSQIAADKVKLGLNVDEQAANEIKQIQAAYERLKSSG
                     TLSSQQLARANELHKQKIRELKGEAASMPSAMNPIAASLQNIGSKAAALTGVTAGLYG
                     VKEGLSAVLRTTQEFQAVRQRLDYAFGGIEEGGKQLDYVRNISNQLGLEVLGAAKGYG
                     SLASATKELNLTHQQTQQIFSGVAHAAAAMHLSADDANGVFLALSQIAGKGKVSMEEL
                     RQQLGERLTPAMGIAAKSMGVTTGELEKMIETGISAEDFLPKFGAALEEVFAGEAAAN
                     ANTLSGQINILNNQFREMLNGFGEGGIADAAIKVMHDIGESITWLETQIAGMDATVTG
                     GVKDAFVGTYEGVKEAGLAIVHIFTSIVERINAVGQAFNTISGNADKDFDFVKSVIDG
                     INITIGVLRDGIAALGISIDLVAATALKGFSLMAEGLSKITFGELSDNFEQAAKGLSE
                     ASEKHFGKAHDAAMNFESKAVQAADRAIESEQQRFARLETEAKAAYQAASQAAIEAAG
                     KAKEAQEQANAAIGTDAEETANKQAAEANKVATAAKVAAARTEKDWKEAHEKIGGSAE
                     EVEKLTEPLRQAGLASQTTAKQLQEIPKELAAAKGAAVGLGLDLRAAMNETSPAINQT
                     KEKVDALQAGFTSMQASGTNAAVLVSQAMTQMLDAAVNPADIEAVKQKWREFGATGAM
                     SASQVEQGLMQAETKLQDINNTLNPVNAALQNTTAKAKELPQALIGAKAAAQTLGVDI
                     TAAMNEPSMAMLTAQRNIDKIRAGFDDMKASGMNAGAVVRGAIDEMLKAAANQSDIDL
                     AKKKIKEFGTTGALSMRDVEQGIINANSRLQEINNTLNPVNQAFEKLGIKTKEALGNA
                     AEDMQAAFMRVKDSGKATTEGLAEGFKRAAEAALASGNIQSQSWVQTHAATYGYKVAI
                     DDTGKASLQLAEDVKKSNAEQQAATKGTTEALKQQAGATSGVASATKSATGAAKSYKA
                     RLTVNNAEAYKNMISAVREQMRFGAPGDVLAFFKEYNRYVKIYQDQTKRAQDATANLN
                     QKIEAGTVNQKDLAAAIDATNVRLGKLDSTTLKNLHEAIDKARQKIKAMRDEAEDTRA
                     GLEAELASARGDETKREALEQQSKIRELNLKLQEAEAAQNREAIGDYRNAIKLQEQIY
                     TEKQRQAALEKERAQQQADQERQATEQAAAQAQAEQTPIEINVGPATVPVEATAGMAN
                     QIANALKGALAGRDQGLVDAAVGQFMNQILDEMKRMGM"
     assembly_gap    9229..9265
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9266..9538)
                     /locus_tag="PK856_00735"
     CDS             complement(<9266..9538)
                     /locus_tag="PK856_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00927.1"
                     /translation="MAKKFEAGLEIKADVQGTQSIVKLASEIDAVGVDASELRTEALA
                     LAQAWKQLENSQALIASFKTLKEQTVQVTTKLSAAQAKVAELAREMK"
     gene            9631..10398
                     /locus_tag="PK856_00740"
     CDS             9631..10398
                     /locus_tag="PK856_00740"
                     /inference="COORDINATES: protein motif:HMM:NF019612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HRL00928.1"
                     /translation="MVNLYDLLSVKPSATVEQIQRALINAAQKRTLNLEQLEKIKVVL
                     LNDSSRAQYDAKLFADYPDIKAQFDTDRAEIENREALQAQKLAQAAKATPETQNSNFI
                     SGAKKMGFGLNKLVQGVLGNMSEVTLEELEQQFNRYLFDQEKITVGYKLVRDVIVFTD
                     QRILFIDKQGASGKKQSFKSIYLMNIVDVEMETAGMGLDDSEISIYCLQNIYRKAHQA
                     QMVKFKFEFPKSTDIVPLYTMLGSLAYQNRLEINQPE"
     gene            complement(10445..11197)
                     /locus_tag="PK856_00745"
     CDS             complement(10445..11197)
                     /locus_tag="PK856_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00929.1"
                     /translation="MARQADNGLIFAGKTYIKNLMIPGAGFVDVGNVTSLALKSESDA
                     KERISKQKGSYGSALDSITTPKPTTFSMKCDTFDRHNLAMALMGQDADFNQAEETLTD
                     QIVEIGQQGQWYSLGKTNIDPSTVAIKTSTDTVVDKTAYQVHAGLGMIQIVDGSTTVQ
                     AGDSIKASFKTKVVKGFQIHAGVLDNLKLEIMVEGENRTTGKTGTLHIPSVSVGADSE
                     IDWFGDDFAESSLAGTVVKSGTNAPYTFTEIE"
     gene            complement(11254..11718)
                     /locus_tag="PK856_00750"
     CDS             complement(11254..11718)
                     /locus_tag="PK856_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00930.1"
                     /translation="MAMQMHDNMLAVYPELLNKVRSIVGVPLVKEASDLAIFMSEAKT
                     QRKVAPAHGAVYVVYGGHAPNDSAGQGKLQKNTLFFTLVYCARYLEGGKSTLYETGAT
                     LTAIKKALQGWDPGKEFVVSPFVESNSPPIEYNDGYAFYPISFTATVCVVAN"
     gene            complement(11721..12152)
                     /locus_tag="PK856_00755"
     CDS             complement(11721..12152)
                     /locus_tag="PK856_00755"
                     /inference="COORDINATES: protein motif:HMM:NF016927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HRL00931.1"
                     /translation="MRLIIQADLTPISQRLTKLVGQMDDLKPVMASIGAVLEGSTRLR
                     IAESKADADGKAWADLASSTKAAKGNKGSILVHRGTLMRGITYEASKDSVIVGSGQLY
                     AKWHQEGTAKMPARPFLGVSATDYQDVDDLLSDWLNGLTGA"
     gene            complement(12152..12568)
                     /locus_tag="PK856_00760"
     CDS             complement(12152..12568)
                     /locus_tag="PK856_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 domain-containing protein"
                     /protein_id="HRL00932.1"
                     /translation="MNIITRDDLLERFGEKELMELTDREAYAVINDNVLQKAIEDAEA
                     EAGAYLQAAGLVLKAPPKALVIKTCDIARYYLYENGVTDIVEARYKQAISWFKDVVKN
                     PQMLDANATPKASNPSRCTVIPNQPPASWADIGGKY"
     gene            complement(12571..>12841)
                     /locus_tag="PK856_00765"
     CDS             complement(12571..>12841)
                     /locus_tag="PK856_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HRL00933.1"
                     /translation="FQFMQYDGEFGAKGAKIAAGEGLVLPLGTRSTFAEYFAPADMNA
                     TVNTKALPYYASREKLQHDKGWSIHAQSNPLPLVLRPGLVATVKA"
BASE COUNT         3087 a         3095 c         2930 g         3692 t
ORIGIN      
        1 tggtgatggt atagaaccca agcgccgctt aaaggccata ggcagtgcat ttgctgtggc
       61 agagcccagt cgattaaaaa acggctgcgg taaagggttg ggtacaaaag ggctaggcat
      121 tgtctattgc cggcttaata taatcccaca caacaggctc atatttttct ttgtcatcga
      181 ctgccatcac cgtataaatc ttactgacat ctaaccaagg tattaagtat gagccgtctg
      241 cattgctcca ggtttgacgc atacagggaa atgcatgaat gcgttcaaac acaataatac
      301 gtttctgact tggtttaccg tctaccgtga caatgccgtt accattgccc gcaataaagc
      361 ctagaagttg tgggtctctt gcgctgtatg cgctcatttc tgtgtcaaca taattcagca
      421 tctcagtccc attctgttaa attaaccatg aagaacatcg gagctgcaag atagtgagta
      481 gcccaaccac tacgggttaa attgcaaatt aaatagtttt taccatcaac tgacttttca
      541 gcgaaatgtt cagttggcat aagtgattga tgaatcaaat aaccgaatgg caaaaagcca
      601 cgcaacatgc ctgcatctgt tacagcaatt tttgaataaa tcacttctga attaattgga
      661 gagttgccaa tcaataaatt atctgtgaat agcgcagatg gaattacttt tacccaattc
      721 gataaaccat taaccgactg tgaaatcagt gaagaatcgc cattccaaaa ttgaatattt
      781 ttgcttgtag tacccccact tccaaagtga cctaaattgg aaattctaaa taaaattgta
      841 ttcccacgag gattgttaac cctgttttca atatcaccaa aaaataaacc tgcaccgttg
      901 gctcttgcct tacctgaaaa tgcccctttg attccaataa tgatgataaa agccttggcg
      961 tgaccgacta atatccattc aatacctgtg gaatgaaaag tggggaatac atctatagct
     1021 ggtgcttttt ctattaaggc tccagccgca tcggtatcca tgtatgcaga tattgttgca
     1081 gttgatgtgg tcgttcctgc aacgtgtatg atgtgccggc cagactccaa atctgttggc
     1141 ctaaatgcgc aatcaaatcc atctgcagat tctgttcctg caactacttc ccacccaagt
     1201 ggctctttct tgttgctacc tgaaccgtat ccagccgcca aacaagcttt aagaactgtt
     1261 ttgaaatcac cacgaccacc aatgatttga ggcgcgtctg tgtctgtgct tctgaatact
     1321 tttactggtg ttcccatttt ctaatcctta tttgtctact gtattgccac gcaagcacat
     1381 ggtgaaaccg tctttgcctg tttgtgcgac tgggctaggt tgtaccgcac gcagcaccca
     1441 agtcggcatt tggctgctga atgtattaaa gcgtatgcaa ttgcctgaca tccaaccgcc
     1501 accgaatgca cctttaggca gagtaaagta tggttggcca gtggcaggat ttggcggggc
     1561 taaatcagtc aatcggtcgc tggttgcaat caaaccaagg ttttcaccta tcacatcaaa
     1621 ctgggcatcg gttttaaaca gcaacagcca gcgctctgta atggccccgt tggatgccaa
     1681 tttgattggg tagtctttca cattcagctt tgccagaatt tcatctgttt ggcgctcatt
     1741 gctccagacc ttgttccaag cctgttgtga aaatggcgcg gtaacacggg taagcaaatt
     1801 accgccaata aaagcactgg atatggtggt gccatcaaca ggataggcac gagatgtggc
     1861 aaattgcagc tttaatgtac cgttgatgtc caagcccaca atgcggtttt cttcttcctg
     1921 cacatggtgt gcagtaattg gcattgtgta ggctgataag tcgatttgag gctcccatgt
     1981 cacggtgccg tcagccaagt caacggtata aagctctgcg ttgatgtgct tggcattggc
     2041 atcaaccaag cacacacggc tcaagtcgtc gcggcttaat tgaatgattt ggccacccgt
     2101 gtgggcgctg cctatacctt gcttgacctt attgccgatg acgatcatgt cgccacgtct
     2161 gaaaatgggc acacggccat cgggcggcag gcgcacggca tcgatgccga tgattgagct
     2221 atcaagtgga atattggatt gcaccacggt gttgtatcgc actgattcag gcgcgaagcc
     2281 tttaacactg gtcaattcat agaagccagt tttggtttcg atggtacctg tgatgtcgcc
     2341 agtgattgcg ccattagcgc ccgcagtgcc aacaatcaaa ccactttggc cattggcgta
     2401 agctgtgaag ctttgaggtt taacaggtgc cgcaagggta taaccatagg tttgcttgcc
     2461 caacagcccg ccaactgcat acacaccggc cagcactttg atggtgccca aggccaatga
     2521 tgggttggta atggccaagt caccataggc tgacagtgtg ccaatatctt caccattgcc
     2581 tgtggtgcta ttgatgttgc ggtacaaacg gcccatgcgc tcataaactt tggtgccgcc
     2641 aacctcaaac acaaacgaat tgggtacaca ttgcagtggc tgtggtttcc ctgtcagtac
     2701 gttggttgag taatagctga tggcttgttt actggaataa acccgtgttg ccgacgattg
     2761 cagcttataa aaataggagc ttggtcctgg caacggtgtg acatcacgac tgaccacctt
     2821 aaaaatgccc cattcaccaa tgctgctttg attcaaaacg gcttcagttg attcccagct
     2881 tggggtaaca cagtcgcctt tgatggagtt gaacggcaca ataaacaggc cggttgaata
     2941 atctatgctt gagctggcca ccagcttacc gttgcgcatt aattggccat tcccattgtc
     3001 ggtaaacaca aacatattgc gttcttgaac ctcaacaaag tcagtaccca tatagttcca
     3061 ggctttgatg tcttcgtggt aatagaggtc ggccacaatc ttggccgttc cgcgcacggt
     3121 tgggccaatg ctgcctgata tttgggtggc tgtggtttca agggtgaaac tggttttagt
     3181 gtactcgtca gcgtagtaat caaagcctat gtctgcttca gcaatactgc taccagcact
     3241 gaggtagatt tggccaccag cgtagtccac gcggcctgta agcgctccag ttaaagcgcc
     3301 ttggccgttg tcagtgactg tgcttgtgcc tgtgttgatt ttgaccgtgc ctggcttaat
     3361 gatgggctgt ggcacttgca gcaaagacag ctctgggctc agtgcgacag gcacaatcac
     3421 gcttccagca tctttgtcgt tgatggtttt gtaaaagtct actggtgccc aagtgatgat
     3481 gatttttgag ccaacatctg gttgctcagg caatgagatg acgacagaac cggtgcgggc
     3541 aactgcgcca cggactttgc cttccttgtc acgcaaattg taatcgccgt ggtcggttaa
     3601 ctcataccaa gtgccttgcg acatccatgc cacagacaca gaaccgttgg cgggcttagg
     3661 gtttaaaagc ggagcccatt ctgtgccctg attggtgtcg tcaatattaa ttatggtaga
     3721 gcaagaggaa ttgctgactt tggccgcagg tacggcctgt accaccgcat tgtacaaagc
     3781 cggtacattt cggataacag cattgttgta atcgattgtg agcacattct caccatcgat
     3841 taactggcct tgagcgttat ccttgtaatt caacaattca acactgccag gcaaaaccga
     3901 ctgattgaga tacaaatcac ccgcgttacg actacccgta aaaacggctt gttttagtcc
     3961 cgtttcaatc caataagccg ttttacttgg gtattggtct atgtaagcgg tttcaaccaa
     4021 gctggttggc acaatctttt caaaaatatc atccacgcga atcgtggctg tactggcggt
     4081 aattggctct accaaaggac gacaaccgta ataagtggct gtatcggcca cttgggtttc
     4141 taaaatcttg gtgggcgatg aagcgtaacc acggccagtt gggtaatcga caccactgaa
     4201 atcacgcgcc aatgggtttg agatttccat tttaatcaca cggcgttgaa attctttgcc
     4261 atcggcgtct tcaaaagtgc gtatttcatg ggtcatggtc aaaatacgga aatactcttg
     4321 tactggtgtt ttgttttttt cttcaaaaga caagcaatag cgttcaccaa caattggcac
     4381 tggcgcttca atgcgttggt atgcctgaat agtacgggta ccagataatt ggcggcccag
     4441 caaagtcatg cgggattcaa tggtaggcac actgtaggct tcaatccgtg gcaagatgtc
     4501 tttacgagat tcgccatagt tttgagcgcc aaacaacaag aatgacacgt tatccatcac
     4561 tgcacgttcc gacacaatgc aatggccacc gtataacggc tcctcatctt ctcgcagcac
     4621 agctgggtaa accaaacgtg cattgaatgc gcccatcgtg cggtcaacat ctgaaactgg
     4681 ggggaataac tcattgtctg cgccagtcaa tgccgtgcca cgcatcaagc cgccaccatc
     4741 atcggtgtcg gtcaagcgct cagaggggta gattttgagg tcttgttggg ttaaacgtgt
     4801 cgtttgctgt gccatgattg aagcctttaa atagtaagct tcagtataaa aaaagacgcc
     4861 gtttaaggcg tcttagggta tgcgagtaca gacggtttac atggtcatca attgaatggc
     4921 cattgtgtat cgatctgcac cagcttcagg ggtcgcataa cgcacaggct caactttatt
     4981 gagagaattg tcatggtaac gaaagcctac cgtaaactga cgaccatcat aatgaattaa
     5041 ggtcattttt aagccaggca cgtctgtcca agcgcgcaat ctctcaacca gtgtgcgacc
     5101 gtaccatacc cagtcaccgt ctaacgtaat tggccgtcct gcttgtttta tgccttgctg
     5161 caccaatacc gccccagcaa gactgtatac aggctgtgtt tgcgctatgg ctgaccaatt
     5221 gaattcatcc gtccaatgca tatcttggtc aaattccagt tcttcgctcg tgtcattgcg
     5281 ttttaatttc caattggcca ttacatgccc atccttttca tttcatctaa aatttgattc
     5341 ataaactggc ccacggcagc atcgaccaag ccttggtctc gaccagctaa agcacctttc
     5401 aaagcattgg ctatttgatt ggccatacct gcggtggctt ctactgggac tgttgcagga
     5461 ccaacattga tctcaatcgg tgtttgctct gcttgggctt gggccgctgc ttgctcagta
     5521 gcttggcgtt cttggtcggc ttgttgttgg gcgcgttctt tttcaagagc cgcttgccgt
     5581 tgcttctcag tgtaaatttg ctcttgcagc ttgatggcgt tgcggtagtc cccaatggct
     5641 tcacggtttt gagcggcttc agcttcctgt aatttcaaat tcagttcacg tatcttggat
     5701 tgttgctcca aggcttcgcg cttggtctca tcgccacgcg cagaagccaa ttcagcttct
     5761 aagccggcac gggtatcttc ggcttcatca cgcatggctt taatcttttg ccgtgctttg
     5821 tcgatggctt catgcaggtt cttcaatgtg gtgctgtcta gtttgcccaa acgcacattc
     5881 gttgcatcaa tggccgcagc aaggtctttt tgattgaccg tgccagcttc tattttttga
     5941 ttgaggtttg cagtggcatc ttgggcgcgt ttggtttgat cctggtatat cttgacgtaa
     6001 cggttgtact ctttgaaaaa agcaagcaca tcaccaggag caccaaaacg catttgctcc
     6061 cgtactgccg aaatcatgtt tttataggct tcggcattgt tgacggtgag gcgggccttg
     6121 tatgacttgg ctgctccagt ggctgacttg gttgcactgg ccacgccgct agtggcgcct
     6181 gcttgttgtt ttaaagcctc tgtggtgcct ttggtggccg cttgttgctc tgcatttgat
     6241 tttttaacgt cttcagcaag ctgcaagctg gctttgcctg tgtcatcaat ggcaactttg
     6301 tagccatagg tcgcagcatg ggtctgcacc cacgattgcg attgaatgtt gcccgatgca
     6361 agcgccgcct cagctgctcg cttaaagcct tcggccagtc cttccgtagt ggctttacct
     6421 gagtccttca cgcgcataaa tgcggcctgc atgtcttccg cagcattgcc cagtgcttct
     6481 tttgttttaa tgcccagctt ttcaaatgcc tggttaacag ggtttaaagt gttgttaatc
     6541 tcttgtaatc ggctgtttgc gttgatgatg ccttgttcaa catcacgcat cgaaagcgca
     6601 ccagtggtac caaactcttt gattttcttc ttagcaaggt caatatctga ctggttcgcg
     6661 gccgctttca acatttcatc aatcgcacca cgcaccacag cgccagcatt catgccactg
     6721 gccttcatgt catcaaagcc agcacggatt ttgtcgatat tgcgctgtgc cgtcagcatg
     6781 gccatacttg gttcattcat ggcagcggtg atgtctacgc ccagtgtttg ggcggcggct
     6841 tttgcaccaa tcaaggcttg gggcagctct ttggctttgg ccgtggtgtt ttgcagtgct
     6901 gcattgactg ggtttaaagt gttgttgatg tcttgcaact tagtctcagc ttgcatcaaa
     6961 ccttgttcaa cttggcttgc cgacattgca ccagtggcac cgaattcacg ccatttttgt
     7021 ttgactgctt caatatctgc ggggttaaca gcagcgtcta gcatttgtgt catggcctga
     7081 ctaaccaaca ccgcagcatt ggtgccgctg gcttgcattg aagtgaaacc agcttgcaat
     7141 gcatccactt tttctttggt ttggttaatg gcgggactgg tttcattcat tgccgccctc
     7201 aaatccaaac ccaagcctac ggccgcacct ttggccgcag ccaattcttt ggggatttct
     7261 tgcaattgtt tggcagtggt ttgtgaggca aggccggctt ggcgcaatgg ttcagttagt
     7321 ttttcgactt cttcagcact accaccgatt ttctcgtgcg cttctttcca gtctttttct
     7381 gttcttgcag cagccacctt agctgctgtg gccactttgt tggcctctgc cgcctgcttg
     7441 ttggcggttt cctctgcatc tgtaccaatg gcagcattgg cttgttcttg cgcttccttg
     7501 gccttgccag cagcctctat ggccgcttgc gatgctgcct gataggctgc tttggcttca
     7561 gtttccaaac gtgcaaagcg ctgttgttct gattcaatcg cgcggtcagc cgcttgaacc
     7621 gccttagatt caaagttcat ggcggcatca tgggcttttc cgaagtgttt ttcagatgct
     7681 tcagataagc cttttgcggc ctgttcaaag ttatcagaca actcaccaaa ggtaatttta
     7741 gacagacctt ctgccattaa agaaaagccc tttaatgcag ttgcagcaac caaatcaata
     7801 ctgataccaa gtgcagcaat gccatcccgt aatacaccaa tggtaatgtt gatgccatca
     7861 atcacacttt ttacaaaatc gaagtccttg tctgcattgc cactgattgt attaaatgcc
     7921 tggccaacag cgttaatgcg ctcaacaatt gaggtaaata tgtggactat ggctaaaccc
     7981 gcttctttga ccccctcata tgtcccaaca aatgcatctt taacgccacc tgtaacagtg
     8041 gcatccatgc cagcaatctg ggtttcaagc catgtaatgg attcgcctat gtcatgcatc
     8101 actttgatgg cggcgtccgc aatgccgcct tcaccaaagc cgttgagcat ttcacgaaat
     8161 tgattgttca ggatattgat ttgaccactc agagtattgg cattggccgc ggcttcgccc
     8221 gcaaatactt cttccaatgc cgcgccaaac tttggcagga agtcttcggc actgatgccg
     8281 gtttcaatca ttttttccag ctcgccagtg gtcacaccca tagacttggc tgcaatgccc
     8341 atagcggggg tcaagcgctc gcccaattgc tggcgtagct cctccatact gactttgcct
     8401 ttacccgcaa tttgactcaa tgccaggaac accccattgg cgtcatcagc agataagtgc
     8461 atagcggccg cagcatgagc cacaccacta aagatttgct gtgtttgttg gtgggtgagg
     8521 ttaagttctt tggtggctga ggcaaggcta ccgtagcctt tggctgcgcc caacacttcc
     8581 aaacccaatt ggtttgaaat attgcgcacg tagtccagct gcttgccacc ttcttcaatg
     8641 cctccaaaag catagtcaag gcgttggcgc acagcctgaa actcttgtgt tgttcgtaat
     8701 acggcgctta atccttcttt gacaccgtac aaaccagctg tgacgccagt taacgcagct
     8761 gcttttgaac cgatgttttg cagtgatgct gcaattgggt tcatggcact gggcatactg
     8821 gcggcttcgc cttttaattc acggattttt tgtttgtgta actcattcgc acgggcaagc
     8881 tgctgactgc ttaatgtgcc gctgcttttg agtctttcat aagcggcctg aatttgtttg
     8941 atttcattcg cggcctgttc atctacattc aggccaagtt tgactttatc ggccgcaatt
     9001 tgactaagcc gctttgcttc ttgtgacaat gcgcttaatt tttcttttgc agcagccgct
     9061 tctttgttga tacgaatata ttcagcacta agatttttaa ctgaaatacc tgcttgatcg
     9121 agcgcagttc ttgtttgacc aagcttctgc ttgagctgtt cttgttgatt tgtgagcgat
     9181 tttgtctttt caatagcggc ttggaaccgt tcctctagct ctgcagtgnn nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnntttca tttcgcgagc caattcagcc accttagcct
     9301 gggctgctga aagtttggtt gttacttgaa cggtttgttc tttcagtgtt ttaaaactgg
     9361 caatcaatgc ctgagagttt tccagctgtt tccatgcttg ggccaatgcc agtgcttcag
     9421 ttcttaattc agaagcatcc acgcctacag catcaatttc gcttgccagc ttaacaatgg
     9481 attgagtgcc ttgaacgtcc gcttttattt ccagaccagc ttcaaatttt ttagccataa
     9541 ctaatgaata taatttgtct ataatgctgc aaattatttc ttttttaatg gtttattgtg
     9601 tctgatgaca caagagtaca agggatgata atggttaatt tgtatgattt acttagtgtc
     9661 aaaccatcgg ctacagttga gcaaattcaa agagcattaa tcaatgcggc acaaaaaaga
     9721 actctgaatc ttgaacagct cgaaaagatt aaagtcgtgt tgttgaatga ctcatcacgt
     9781 gctcaatacg atgcaaaatt atttgcagac tatcctgata tcaaagccca atttgatact
     9841 gatagagctg aaattgaaaa tcgtgaagca ttgcaggctc aaaagcttgc acaagcagct
     9901 aaagcaacac cagaaacaca aaatagtaat ttcatttcgg gagcaaaaaa aatggggttt
     9961 ggattaaata aattggtcca aggtgtcctt ggcaatatga gcgaagtcac tcttgaggag
    10021 ttggaacaac aatttaaccg ctacctattt gatcaagaaa aaattactgt tggctataaa
    10081 ttagtgcggg atgtcatagt ttttacagat caacgaattc tattcattga taagcaaggt
    10141 gcatcgggga aaaaacaaag ttttaagtcc atttacttga tgaatatcgt ggatgtggaa
    10201 atggaaaccg ctggtatggg cttggatgac agtgagatca gtatttactg cttacaaaat
    10261 atttaccgaa aagcccacca ggcgcaaatg gttaaattca aatttgaatt tccaaagtcg
    10321 actgacattg tgccgcttta cacaatgctg ggaagcttgg cttatcaaaa caggcttgaa
    10381 atcaaccaac cggaataata aaaagcggcc tgtaatgatt acaggccgct tttatcaaaa
    10441 agacttactc aatttctgtg aacgtatatg gcgcattggt accgcttttg accacagtac
    10501 ccgccaaaga cgattcagca aaatcatcac caaaccagtc aatttcagag tctgcaccta
    10561 ccgatactga tggaatgtgc aatgtgccag ttttacccgt ggtgcggttt tcaccttcga
    10621 ccatgatttc caatttcaag ttatccaata cacccgcgtg aatttggaag cctttcacca
    10681 ctttggtctt gaaagaggct ttgattgagt cgccagcttg cacagttgta ctgccatcaa
    10741 caatctgaat catgcccaag cctgcatgaa cctgataagc cgttttgtcc accacggtat
    10801 cggttgaggt tttaatggcc acggtgcttg gatcaatgtt ggttttaccc aatgaatacc
    10861 actggccttg ctgcccaatt tcaacaatct ggtcagtaag ggtttcttcg gcttgattga
    10921 aatctgcatc ttgacccatc aatgccatgg ccaagttgtg acggtcaaac gtgtcacact
    10981 tcattgagaa ggtggtgggt ttaggggtcg tgatgctgtc caaagcactg ccatacgaac
    11041 ctttctgctt gctgatgcgc tctttcgcgt cagattctga cttcaaagcc aaagaggtca
    11101 cgttgcccac atccacaaaa cccgcaccag gaatcatcaa atttttgatg tacgttttac
    11161 ctgcaaatat caggccattg tctgcttgtc gtgccatgtc tttactccat ttctaaatat
    11221 tgttacaaag gttttaatgc ggtttaaaag cggttaattt gcgaccacac aaacggtggc
    11281 ggtaaagcta attgggtaaa aggcataacc atcgttgtac tcaatcggtg gcgaattgct
    11341 ttccacaaac gggcttacta caaactcctt accaggatcc caaccttgca atgctttttt
    11401 gatggccgtt agagttgcac ccgtttcata cagtgttgac ttgccgcctt ctaaatagcg
    11461 tgcgcaataa accaaggtga aaaacaaagt gttcttttgc agttttcctt ggcctgctga
    11521 gtcgtttggg gcatggccac cgtaaaccac atacactgca ccatgagcgg gtgcgacttt
    11581 gcgctgtgtt ttggcttcgc tcatgaagat tgctaagtca ctggcctctt taaccaaggg
    11641 cacaccgaca atactgcgaa ccttgtttaa taactcaggg tacacagcca gcatattgtc
    11701 atgcatctgc atagccatat ttatgctcct gtcaagccgt taagccagtc agacagcaaa
    11761 tcatccacat cctggtagtc agtcgctgac acgcccagaa atggccttgc gggcattttg
    11821 gcggtgcctt cctggtgcca cttcgcatac aactgaccag agccaacaat cacactgtct
    11881 ttactggctt cgtatgtaat gccacgcatt aaggtgccac ggtggaccaa gatgctgcct
    11941 ttgttgcctt tggctgcttt ggttgagctg gctaaatctg cccatgcctt gccatctgca
    12001 tcggctttgg attcggcaat acgcaagcgt gtactgcctt ccaaaacagc accgattgat
    12061 gccatgacgg gctttaaatc atccatctgg ccaaccagct tggtaagccg ctggctgatg
    12121 ggggttaaat cagcttgtat gattaaacgc atcagtattt gcctccaata tcggcccatg
    12181 atgctggtgg ctgatttgga atcactgtgc aacgggatgg attgcttgcc ttgggggtgg
    12241 cattggcatc aagcatttgc gggtttttaa ccacgtcttt aaaccagctt atggcttgtt
    12301 tgtaacgggc ctcaacaatg tcagtcacac cattttcata caggtagtaa cgtgcaatgt
    12361 cgcaagtctt gataaccaat gctttaggcg gtgcctttaa aaccaagcca gcggcctgta
    12421 aatatgcacc cgcttcagct tctgcgtcct caatggcttt ttgcaacaca ttgtcattga
    12481 tgactgcata ggcttctcgg tctgtgagct ccatcaactc tttttcgcca aagcgctcaa
    12541 gcaaatcgtc gcgggtgata atgttcatgg ctatgctttc actgtcgcaa ccaaaccagg
    12601 acgcaacacc aatggcaacg ggttagactg cgcatggatg ctccaaccct tgtcatgttg
    12661 caacttttca cggctggcgt aatacggcaa tgctttggtg ttcacagtgg cattcatgtc
    12721 agcgggagcg aagtattctg caaacgtgct gcgtgtaccc aaaggcaata ccaagccttc
    12781 acccgcggcg atttttgccc ctttcgcacc aaactcaccg tcgtattgca tgaactgaaa
    12841 g
//
LOCUS       DAOMVK010000009        12384 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_1166_length_12384_cov_3.316084, whole genome shotgun sequence.
ACCESSION   DAOMVK010000009 DAOMVK010000000
VERSION     DAOMVK010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 12384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12384
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1166_length_12384_cov_3.316084"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PK856_00770"
     CDS             complement(<1..370)
                     /locus_tag="PK856_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HRL00934.1"
                     /translation="MSHTRYAFIVSDRTGITSESMGQALLNQFSHIEFQRSMHPFIDT
                     VDKAEKMVAIINQAAEESAVRPLIFSSIVNDDVREVIHQSNGLHLDFFDAFIGILEEE
                     LQTEATNIVGNIHGITDEEKY"
     gene            983..3370
                     /gene="ppsA"
                     /locus_tag="PK856_00775"
     CDS             983..3370
                     /gene="ppsA"
                     /locus_tag="PK856_00775"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HRL00935.1"
                     /translation="MSGNNVIWFENLRNTDVEQVGGKNASLGEMISQLASKGVRVPGG
                     FATTADAFRKFIAHEGLNDRINAALDALDVEDVNALAECGKNIRQWVMDTPFPAELDA
                     DIQTAWEKMVADSGTEEISVAVRSSATAEDLPDASFAGQQETFLNIKGLANVKEAMHH
                     VFASLYNDRAISYRVHKGFAHADVALSAGVQRMVRSDSGASGVMFTIDTESGFDQVVF
                     VTSSYGLGEMVVQGAVNPDEFYVHKNNLREGRPAVLRKVMGSKLIKMVFTEDASAGKS
                     VKIEDVPEEMRREFSISQDEIQELAKYALTIEEHYGRAMDIEWGRDGIDGKIYILQAR
                     PETVKSQEGRQDTLRRYRINEKGAVLVEGRAIGQKVGQGQVRLIKDASEMDTVKAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATELLKEGQM
                     VTVSCAEGDTGLVYDGKLEVEIIDLQLDSMPEAPAKIMMNVGNPELAFGFASLPNEGI
                     GLARMEFIINRQIGIHPRALLELNEQDADLQKTIKDRITGYASPRDFYVDKIAEGVAT
                     LAAAMYPKKVIVRTSDFKSNEYANLIGGSLYEPHEENPMLGFRGAARYVSPEFKDCFA
                     MECEALKRVRDDMGFTNVEIMIPFVRTLREAEAVVNLLRENGLERGKNGLRLIMMCEL
                     PTNAVLAEQFLQFFDGFSIGSNDMTQLTLGVDRDSGGPIASTFDERDPAVKVMLSLAI
                     NACRKLNKYVGICGQGPSDHPDLAKWLVEQGIETISLNPDTVIETWLYLAKELKK"
     gene            3528..3965
                     /locus_tag="PK856_00780"
     CDS             3528..3965
                     /locus_tag="PK856_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00936.1"
                     /translation="MAIFKSRVYQACLGLCLLLPVLSAHASMETKCYEPKVKADYTEY
                     ECRYGLYADIRKAYPKRINDLKSRIPEKYHAYLPTSVPTQSTRIHINEHEYLSVYVDH
                     YHTEMRIILMENEEMKFRYFIENDRRYFFSRGATPGMILRVMH"
     gene            4126..4527
                     /locus_tag="PK856_00785"
     CDS             4126..4527
                     /locus_tag="PK856_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00937.1"
                     /translation="MKTAMKCAWMGMLLALAMPAFADDTQTTYCKIQGQLAETTLIGR
                     YLGKSQTDAMQVVVRATDGMDDPFEQNIFIMLMGEIVDGVYERELMAAPEQHEAEFLA
                     EARGLGKTVQDNCMQMDVKQVLKAMKEGYHR"
     gene            4682..5038
                     /locus_tag="PK856_00790"
     CDS             4682..5038
                     /locus_tag="PK856_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00938.1"
                     /translation="MKHLWLCGLLLVALPSLAKMNEDMLCQMVGELAETSLEQREQGV
                     SQDDALQNVYQRTERIDQPFVRGLVNGLMAEVVNDAYQQDGQAADGNDAFRQRQTLAQ
                     VVQNKCKQAGLGAFLR"
     gene            5131..5535
                     /locus_tag="PK856_00795"
     CDS             5131..5535
                     /locus_tag="PK856_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6232 family protein"
                     /protein_id="HRL00939.1"
                     /translation="MDLEKLNHNLVSQDVQFDATGIIVSGQHHAYADIVSVHYMKPAK
                     RGALRGGMFAVTVALAAWVVLATQQQSQSAKLAVALLLALVAVRLYLFAKVFSKLSLV
                     LKNGETVLVTKKRDMIASDRIYEQLMQHKAKS"
     gene            complement(5726..6274)
                     /locus_tag="PK856_00800"
     CDS             complement(5726..6274)
                     /locus_tag="PK856_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00940.1"
                     /translation="MKQHPYYVSLFVMGALALAGCNDKESVAQAQESVKTTSAVQAKP
                     AQAASEHAPAPASENQADSSGLPFPSDGCTQEAHSSFFEWFVYADPKQREKYIAKELM
                     GKVEPFKIGLLDYRWVYTGANSSGDELIEVTQKASPNAFDVTFVKAEFNEEDEVVKRI
                     GKPGVYQFKLIDGCWKLTGEKS"
     gene            complement(6371..6823)
                     /locus_tag="PK856_00805"
     CDS             complement(6371..6823)
                     /locus_tag="PK856_00805"
                     /inference="COORDINATES: protein motif:HMM:NF022400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HRL00941.1"
                     /translation="MSDNTPNPYQSSVVVSNEDSHAGGNHDLIDSLDVSESWKRKFHI
                     IDDLNSIKDPKEKKAYFKSLTWGERFGQSNIWAFLLGCIYFFCKGMWKPGITFFCISV
                     VLLVLMVVLNIDNDAVARAVGIGFGCLAMTRANVLYYTKKVLGKDLWI"
     gene            7063..8256
                     /locus_tag="PK856_00810"
     CDS             7063..8256
                     /locus_tag="PK856_00810"
                     /inference="COORDINATES: protein motif:HMM:NF018485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL00942.1"
                     /translation="MTHTQQSAYAPWLVIAAGIVAAMHIGKLPPTIPVLQQQLSLSLN
                     QSGFLLAAIQVAGIAMALFIGLMAEKIGLKRLQVGGLLVLAVASFLGSYAGSFTALFA
                     ARLLEGFGFLSIVLAGPSLIKHLVPAQKLKPVLGFWAAYMGLGVATSLILAPNLLQHL
                     TWPYVWLLYALLALVMAGLVWRWVPNVRLQSHADAPPLRQLLLQTLSHPAPWLLGILF
                     ASYTSQWFAITSFLPTIYAHAYIPASQAGMLTALVCLSNVGGPIVAGMMLQQGRPAWV
                     LLTAGFVLMMLGAVLTFAGEGFLPFSVRYCGVLLFSACGGLIPGVVFAYVVYLAPSQQ
                     TVASTMGWVQQWSALGQFSLPPLMGSLVMWWGSWRNAWLVCVALGVVGIVCALGIRRL
                     VNRLA"
     gene            complement(8319..9407)
                     /locus_tag="PK856_00815"
     CDS             complement(8319..9407)
                     /locus_tag="PK856_00815"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF004127.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRL00943.1"
                     /translation="MSIDVNDSVLLATTLPTASGQHLGILTLNRPKALNALNLNLIQA
                     LRAQLSAWQHDANIVAVWLQGSDKALCAGGDIREVQQYNASASSPEAALKQASDFFQA
                     EYGLYADMANYAKPIVTWCGGIVMGGGLGLAAASSVRIVTETTLMAMPEISIGLFPDA
                     GANWFLQRFPFQSGLFLGLTGSRFNGSDAMLGNLAEFALSSQARESTLAALCQAQWHG
                     SAHEVIAQTLLTQARQSLPPSQILAHQADIIQVVSQATFQQALQQVTLLAPKSTWLQA
                     AADYVAQGCASSAGLHWLLMQRCRYWSLEAVLNLEHTVASHCVTRPDFIEGVRALLID
                     KDKQPHWHKSVAEVDEAWLMSFLQALPQ"
     gene            complement(9640..10029)
                     /locus_tag="PK856_00820"
     CDS             complement(9640..10029)
                     /locus_tag="PK856_00820"
                     /inference="COORDINATES: protein motif:HMM:NF019008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HRL00944.1"
                     /translation="MFKSAISAFILATSFSFVISTTVQAADLSHDMHQLKSGYKAFSK
                     AGNAGQALAALNQMQQAAQSAKQATPEGVDPSNAQQVAAYQDQLNQLSATISAANDLV
                     QAGKLADAKALGKKMLQIRDSAHEQFR"
     gene            10226..10873
                     /locus_tag="PK856_00825"
     CDS             10226..10873
                     /locus_tag="PK856_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00945.1"
                     /translation="MKPIVYLGAALVSSMMLSACFTSVYAINRLENGSLPETQRNLNA
                     WARTNPNAHRSASLQVSIEGDAPAGLQALPLQLDYVADNCGFKGRSVEGLLEAYYPHQ
                     RVNIPVSAATHQTYHYVYQPDAFLPQKDEHGRTCVWRVTHAQVQLKAINPASKSVANI
                     QFSLFDKDLNQTQTLTKYFGRQTEGDQFPNITLKSAQVQPEQAFRVMVRHQPEND"
     gene            complement(10943..12058)
                     /locus_tag="PK856_00830"
     CDS             complement(10943..12058)
                     /locus_tag="PK856_00830"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF004127.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRL00946.1"
                     /translation="MNTPLVLFSTKQTSHNQVIAIATLNRPKSLNAQNTALILALRVQ
                     LEAWEHDDSVATVWLDGEGDKSFCAGGDIRELRLGVVEAADEAAAHQHVHTFFAAEYY
                     LCEYISRYPKPIVCWATGIVMGGGMGLASAASHTIVTETTVMAMPEISIGLYPDAGGT
                     WFLQQFPFGSGTFLGLTGSRFNGSDALLGNVAVASRPADSRVATLQALVAADWQDNAQ
                     AVVRRVLNGLDVADLPESQIMAQQTVVQAVVSQATFQDALAVLAEHVEQDAWLAQAHV
                     NTQAGCPTSMGLIWRLLRDMKHASLAECIAQELVVSQQCCTRPDFLEGVRALLIDKDK
                     NPHWVKAVAEVDEAWLDTFFVPSAQLPVFTPANINAS"
BASE COUNT         3080 a         2735 c         3293 g         3276 t
ORIGIN      
        1 gatatttctc ttcatcagtg atgccgtgaa tgttgcccac gatgttggtg gcttcggttt
       61 gcaattcttc ttctaaaata ccaataaacg catcaaaaaa gtccaaatgc aggccgttgc
      121 tttggtggat gacttcgcgc acatcatcgt tgacgatgct ggagaaaatg agcggacgca
      181 ctgccgattc ttctgcggct tggttgatga tggccaccat tttttccgct ttgtccacgg
      241 tgtcgataaa cgggtgcatg ctgcgttgga attcaatgtg gctgaattgg ttgagtaagg
      301 cctgtcccat gctttctgag gtgatgccag tgcgatcgga gacgatgaag gcgtaacggg
      361 tgtgactcat gttgaaaatc cattcactga ttacgcgcat catagcataa agcactccat
      421 ggctagggat gcttgcggtg caaacgggca ataatcgagt gacttattca cccaaacatt
      481 gtattgacag tgaatgcggg agtgatttgc gctgggtggg attttgacaa ttcagttaat
      541 gtgcgcatag gtcagcacag acagcatgaa cagggcgata cgtggtatca gtagagattt
      601 taattgtcat gtaaatggtt tacaggccgc atagtgatgg gtagattgaa attgacgaac
      661 ttgaactggg ttgaaaatgg atggtattca gtgcgattac tcgatggaat agcagacaat
      721 ttctaggtaa acttagatgg gttaaaaatt taaattctgt gaaatttgga atttaatgtt
      781 ttgttatgtg aatttaacta atttaaattt taagattttc cattaattgc cataagtgtt
      841 gcgaactgtg tcactatcgt gctgcgcagc agcaataaag ctgacacatg gcagtcgttg
      901 cggctaaaat acatgggatt ttttgcacag ttttgatgtc gcatcaacat tcttttatca
      961 atgtatttgg aaacaacata ttatgtccgg taataacgta atctggttcg aaaatctacg
     1021 caatactgac gtggagcaag taggcggtaa aaatgcttca ttaggtgaaa tgatcagcca
     1081 attggcttca aaaggcgtac gcgtacctgg tggttttgca actacggctg acgctttccg
     1141 caaattcatc gcccatgaag gtttgaatga ccgcatcaat gcggctttgg atgctttgga
     1201 cgttgaagat gtcaacgctt tggctgagtg cggcaaaaac atccgccaat gggtgatgga
     1261 cacgccgttt ccagctgaat tggatgccga cattcaaact gcatgggaaa aaatggttgc
     1321 cgactcaggc actgaagaaa tttcagtggc tgtgcgctct tctgctacgg ccgaagactt
     1381 gccagatgcc tcttttgccg gtcaacaaga aaccttcttg aacatcaaag gtttggccaa
     1441 tgtgaaagaa gccatgcacc acgtgttcgc ttctttgtat aacgaccgcg ccatctctta
     1501 ccgtgtacac aaaggctttg ctcacgctga cgtggctttg tctgcgggtg tacaacgcat
     1561 ggttcgttct gattcaggtg cttctggtgt gatgtttacc atcgacaccg aatctggttt
     1621 cgaccaagtg gtgttcgtga cttcatctta cggtttgggc gaaatggtcg ttcaaggcgc
     1681 ggtgaatcca gacgaattct acgtacacaa aaacaatttg cgcgaaggcc gtcctgcggt
     1741 attgcgcaaa gtgatgggtt ctaaactcat taaaatggtc ttcactgaag atgcttctgc
     1801 tggtaaatca gtgaaaatcg aagacgttcc tgaagaaatg cgtcgcgaat tctcaatttc
     1861 tcaagacgaa atccaagaat tggccaaata cgcattgacg attgaagagc attacggtcg
     1921 cgcgatggac atcgaatggg gccgtgatgg catcgatggc aaaatctaca tcttgcaagc
     1981 gcgccctgaa accgtgaaat cacaagaagg ccgtcaagac actttgcgcc gctaccgcat
     2041 caatgaaaaa ggtgccgtgt tggtagaagg ccgcgccatt ggtcaaaaag taggccaagg
     2101 ccaagtgcgc ttgatcaaag acgcgtctga aatggacact gtgaaagcag gcgacgtgtt
     2161 ggtaaccgac atgaccgacc cagattggga accggtaatg aaacgcgctt ctgccatcgt
     2221 caccaaccgt ggtggccgta cctgtcacgc tgcaattatt gcgcgtgaat tgggcattcc
     2281 tgctgtcgtg ggttgtggcg acgcgactga gttgttaaaa gaaggccaaa tggtcaccgt
     2341 gtcttgtgcc gaaggcgaca caggcttggt atacgatggc aaattggaag tggaaatcat
     2401 cgacttgcaa ttggattcta tgccagaagc tcctgccaaa atcatgatga acgtgggtaa
     2461 tccagaattg gcattcggtt tcgccagctt gcctaacgaa ggcatcggct tggcgcgcat
     2521 ggaattcatc atcaaccgtc aaatcggcat ccacccacgt gccttgttgg aattgaacga
     2581 acaagacgct gatttgcaaa aaaccatcaa agaccgtatc accggttacg cttctccacg
     2641 tgatttctac gtagacaaga ttgccgaagg tgtggccacg ttggcagcgg caatgtaccc
     2701 gaaaaaagtc attgttcgta cctctgattt caaatcaaac gaatacgcta acttaattgg
     2761 cggcagtttg tacgagccac acgaagaaaa cccaatgttg ggcttccgcg gcgccgctcg
     2821 ttacgtgtct ccagagttca aagactgctt cgcgatggaa tgtgaagcct tgaaacgcgt
     2881 gcgcgatgac atgggcttta ccaacgttga aatcatgatt ccattcgtgc gcaccttgcg
     2941 cgaagccgaa gccgtggtga acttgttgcg tgaaaacggt ttggaacgtg gcaaaaacgg
     3001 tttgcgtttg atcatgatgt gcgaattgcc gactaacgct gtgttggctg aacaattctt
     3061 gcaattcttc gatggcttct ctatcggctc taacgacatg acccaattga ctttgggcgt
     3121 ggaccgcgac agcggtggcc caatcgcttc tacttttgac gagcgcgatc ctgccgttaa
     3181 agtgatgctg agcttggcca tcaatgcttg ccgcaaattg aacaaatacg tgggcatctg
     3241 tggccaaggc ccatcagacc acccagattt ggccaaatgg ttggtagaac aaggcattga
     3301 aaccatttct ttgaacccag acaccgtgat tgaaacttgg ttgtacttgg caaaagaatt
     3361 gaaaaaataa tgcttgaata aagcatgtaa gaagcggcct gtaactcagt ttacaggccg
     3421 ctttgttgta tttggcatca gcacagaaaa cgctgtcttg ttaaggtttt gctaagcatg
     3481 ttttaatagg acaatgacac caaattgaaa agaagctaag gagttgcatg gcaatattta
     3541 aaagtcgggt atatcaagct tgcttaggct tatgcttgct gctgcccgtg ctgtccgctc
     3601 atgccagcat ggaaaccaaa tgttacgagc ctaaagttaa agctgattac actgagtatg
     3661 agtgtcgcta tggcttgtat gccgacatac gcaaagccta tcccaagcgc atcaatgatt
     3721 taaaatcgcg cattcctgaa aaataccatg cctatttgcc taccagtgtg ccaacacaaa
     3781 gcacccgcat ccacatcaat gagcatgagt atttgagcgt gtatgtggat cactatcata
     3841 ccgaaatgcg cattattttg atggagaatg aggagatgaa atttcgatac ttcattgaaa
     3901 atgaccgcag gtattttttc tcacgtggag cgacaccagg tatgatttta cgggtgatgc
     3961 attgagcctg ctgtgaatac cttgctaaca ttgtcatggg gcttttcaat ctgactttca
     4021 atgcggcctg taaatcagtt tacaggccgc attggtgtgt ttgtcagctg ttgtgatgcc
     4081 catcagtcat gtgtttaaat gcgcgcagcc aattaaggat ttcagatgaa aacagcaatg
     4141 aaatgtgcat ggatggggat gttgttggca ttggccatgc ctgcgtttgc cgatgacacc
     4201 caaaccactt attgcaaaat acaaggccag ctggcagaaa caacgttgat agggcgttat
     4261 ttgggcaaat cgcaaaccga tgcgatgcaa gtggtggtgc gcgcaaccga tggcatggat
     4321 gacccgtttg agcaaaatat tttcatcatg ttgatgggtg aaattgtgga tggggtgtat
     4381 gagcgcgaat tgatggcagc gcctgaacag cacgaggctg agtttttggc cgaggcgcga
     4441 ggcttgggca aaaccgtaca agataactgc atgcagatgg atgtgaagca agtgctgaaa
     4501 gccatgaaag aaggctatca tcgctagcag cacaaattga atattttcct gtgaaaaaga
     4561 cagcggcctg tacatttgac aggccacttg tgttgttagg gttaacgcca aaggcttaca
     4621 agggtttctt ggaatttagg tttgtgttct aatttgccat catcttgttg gggagagaat
     4681 catgaagcat ctgtggctgt gtggtttgtt gttggtggct ttgccgagtt tggccaaaat
     4741 gaatgaagac atgttgtgtc aaatggtggg ggagttggcc gagacttctt tggagcagcg
     4801 cgagcaaggt gtgagtcaag acgatgcgct gcaaaacgtg taccaacgca ctgagcgcat
     4861 agatcagcca tttgtgcgcg gtttggtgaa tggtttgatg gccgaagtgg taaacgatgc
     4921 atatcagcaa gatggacaag ctgctgatgg caatgacgcc tttcgtcaac gccaaacctt
     4981 ggcccaagtg gtacaaaaca aatgcaaaca ggctggattg ggtgcatttt tacgttaggc
     5041 gcagtttcaa aacgatgaca ggccgcatgg gtttggcgtg ggcggggatt tttgttacag
     5101 tgcggcattg ctttaaagac aggctctttc atggatttag aaaagttaaa ccacaacctt
     5161 gtgtcacaag atgtgcagtt tgatgcgaca gggattattg tgtctgggca acaccatgcg
     5221 tatgccgaca tagtcagcgt gcattacatg aagccagcca agcgtggtgc gctgcgcggt
     5281 ggcatgtttg ctgttaccgt ggctttggcg gcatgggtgg tattggcgac acaacaacaa
     5341 agccagtcgg caaaattggc agtggccttg ctgctggcat tggtggcggt gcgtttgtat
     5401 ttgtttgcca aagtgttttc caaactcagc ttggtgttga aaaatggtga gacagtgttg
     5461 gtcaccaaaa agcgggacat gattgcttcg gatcgcattt atgaacagct gatgcagcac
     5521 aaagccaaat catgaggaca ttaaaaagcg gcctgtaaat gtttacaggc cgctttttaa
     5581 tgggattgaa tcaatcagca gtcaatatca tctattggct gagctgagtc tgctgtttga
     5641 ggcgtggcaa aagctgattt gccaacacct cagcattaca acggttacgc ttttattgaa
     5701 gatttgatca agttaaggtc aaatctcatg atttctcgcc tgtcagtttc cagcagccat
     5761 cgatgagctt gaattggtac acaccaggtt tgcctatgcg tttcaccact tcgtcttcct
     5821 cattgaattc agctttgaca aaagtcacat caaaggcatt gggactggct ttttgagtca
     5881 cttcaatcaa ttcatcacca ctgctattgg caccagtgta gacccaacgg tagtcgagaa
     5941 gaccaatttt aaaaggctct accttgccca ttaactcctt ggcaatgtat ttttcccgtt
     6001 gtttgggatc tgcataaaca aaccattcaa aaaatgacga atgggcttct tgggtacagc
     6061 cgtcggaagg gaaaggcaag ccgctggagt cggcttggtt ttcagaagct ggggcagggg
     6121 catgctcaga ggctgcttga gccggctttg cttgcaccgc gcttgtggtt ttcaccgact
     6181 cttgcgcctg agccacgctt tccttgtcgt tacagcctgc caatgcgagc gcacccatga
     6241 caaacagcga gacataataa gggtgttgtt tcatatcatc tccaatgcgt atcgaaacct
     6301 aaaaatactg gcgtgcttca aacctgatgg gcagattgtt gtgcatgaga gctttggtta
     6361 tgccaaaggc tcaaatccac aaatctttac ccaatacttt tttggtgtag taaagaacgt
     6421 tggcacgggt catggcaagg cagccaaaac cgatgccaac cgcccgcgca accgcatcgt
     6481 tgtcgatgtt taaaaccacc atcaacacga gtaacaccac agagatgcag aaaaaggtga
     6541 tgcccggttt ccacatgcct ttgcagaaaa aatagatgca gccgagcaaa aaggcccaaa
     6601 tattcgattg accaaaacgc tcaccccagg tcaggctttt gaaataggct tttttctctt
     6661 ttgggtcttt gatgctgttc aaatcatcta tgatgtgaaa tttgcgtttc caactttcac
     6721 tgacatccaa gctgtcaatt aagtcgtggt tgccacctgc gtgactgtct tcattgctga
     6781 ccaccacgct agactggtaa ggattggggg tgttatcgct catgaacatc tctctgatta
     6841 aagttgcata aaatgggcta agccaactat gtgcatgata ctgagtttcg actcagatag
     6901 agagcgtggg cattaatatt tttcaatgaa tgcatgaaat tcaaaataaa aaacctattg
     6961 gcattgtgag gcgctccgtt tttcgggcat catgatggta aacactaaag gtgggcacca
     7021 gccatgtgct aggtcgctaa aacccaaaac agaggaatga atgtgaccca cacccaacaa
     7081 tcggcttatg cgccttggtt ggtcatcgcg gcgggcatag tcgcggcaat gcacattggt
     7141 aaattgcccc caacgatacc tgtattgcaa caacagctgt ctttgagctt gaaccaatcg
     7201 ggatttttac tggccgccat acaggtggca ggcattgcca tggccttgtt cattggtttg
     7261 atggctgaga aaattggttt gaaacgctta caagtgggtg gcttgttggt gttggctgtg
     7321 gcgagttttt taggatcata cgctggcagc tttactgcct tgtttgcggc aagattgctt
     7381 gagggttttg gatttttgag cattgtgttg gcggggccat cgttgattaa acacttggtg
     7441 cctgcccaaa aattgaaacc tgtactgggc ttttgggcgg cgtatatggg tttgggggtg
     7501 gcgacaagtt tgatattggc ccccaattta ttgcagcatc tgacttggcc gtatgtttgg
     7561 ttgttgtatg cgctgttggc cttggtgatg gcgggattgg tatggcgttg ggtgccgaat
     7621 gtgcggctgc aatcccatgc agatgcgccg ccactgcgcc aattattact acaaacctta
     7681 agccatcctg cgccgtggtt gttgggcata ttgtttgcca gttacaccag ccaatggttc
     7741 gccattacca gcttcttgcc taccatttat gcccatgcat acattcctgc cagccaagcg
     7801 ggcatgttga cggctttggt gtgtttgagc aatgtgggcg gccccattgt ggcaggcatg
     7861 atgttgcaac agggtcggcc tgcttgggtc ttgttgaccg cagggtttgt gttgatgatg
     7921 cttggagctg tgttgacatt tgcaggtgaa gggttcttgc cattttcggt gcgctattgt
     7981 ggggtgttgc tgttttcggc ttgtggcggt ttgatacctg gcgtggtgtt cgcctatgtg
     8041 gtgtatttgg cgccgagcca gcaaaccgtg gccagtacca tgggctgggt acagcaatgg
     8101 tcggctttgg gacaattcag cttgccgcca ttgatgggca gtttggtgat gtggtggggc
     8161 agttggcgca atgcttggtt ggtgtgcgtg gcattgggtg tggttggcat cgtctgtgct
     8221 ttgggcatac ggcgattggt aaaccgattg gcgtgatgca tgagataaaa caagcggcct
     8281 gtaaacgttt acaggccgct tgtttttgta ttcaaagctt attgaggcag ggcttgtaag
     8341 aatgacatca gccaagcttc atcgacttct gccacggatt tgtgccaatg cggctgcttg
     8401 tctttgtcta tgagcaaggc gcgcacgcct tcgataaagt cggggcgggt cacgcaatgg
     8461 ctggccactg tgtgttccaa attcaagacg gcttccaaag accagtaacg acagcgctgc
     8521 attaacagcc aatgcaaacc cgcactgctg gcacaacctt gtgccacata gtcagcagcg
     8581 gcctgtaacc aagtactctt gggcgccaaa agtgtgactt gctgcaaggc ttgttggaat
     8641 gtggcttggg acaccacttg gatgatatcc gcttggtgcg ccaagatttg tgaaggaggc
     8701 aagctttggc gtgcttgggt caacagggtt tgggcaatga cttcatgggc cgaaccgtgc
     8761 cactgggctt ggcacaaggc ggccaaagta gattcgcgtg cctgtgatga gagggcgaat
     8821 tcggccaaat tgcccagcat tgcatcgctg ccattaaagc ggctgcccgt taaacccaag
     8881 aacaggccgc tttgaaaggg gaaacgctgt aaaaaccaat tcgcacccgc gtcaggaaac
     8941 aagcctatgc tgatttcggg catggccatc aaggtggttt cggtgacgat gcgcacgctg
     9001 gaagctgcgg ccaagcccaa gccgccgccc atgacgatgc cgccacacca agtgacgatg
     9061 ggcttggcat agttggccat gtcggcatag aggccatact cggcctgaaa gaaatcactg
     9121 gcttgtttta aggccgcttc ggggctgctt gcgctggcat tgtattgttg tacttcgcgg
     9181 atgtcgccac cggcacacaa tgctttgtcg ctgccttgca gccacaccgc cacaatgttg
     9241 gcatcgtgtt gccaggccga caattgggca cgcaaggcct gaatcaaatt caaattcaag
     9301 gcgttcaagg ctttggggcg gtttaaggtg aggatgccca agtgttggcc tgaggcagtg
     9361 ggcaaggtgg tggccagcaa cacgctgtcg ttcacatcaa tactcatgtc gggtctttca
     9421 tcaaagcagt aatgggtttg cccatgaatc taccacataa tcgctcaaga cttgttgcga
     9481 ccatcgccat tgctggctag cgttgcacag actaagaaaa agccgctgac agcggcctgt
     9541 aactggagag gatagaaaaa tcctgatgat tttcaaatgg aacccaccca ttgaagcaaa
     9601 cgtgatgcag aggtgtagac acgcttgtgt tgcgggtatt tagcggaatt gctcgtgggc
     9661 gctgtcccgt atttgcagca tttttttgcc caaggctttg gcatcggcca atttgcccgc
     9721 ttgcaccaaa tcattggcgg cgctgatggt ggcactcaat tgattgagtt ggtcttggta
     9781 ggcggccact tgttgggcat tgctggggtc tacgccttct ggagtggctt gcttggccga
     9841 ttgggctgct tgttgcattt ggtttaaggc tgccaaggct tgtccagcat tgcctgcttt
     9901 gctgaaggct ttgtagccac ttttcaactg gtgcatgtca tgggataaat cggcggcttg
     9961 gactgtggtg ctgattacaa aactgaaact ggtggccaag atgaaggctg aaatcgcaga
    10021 cttgaacatg gtgtgtcctt tgtttataac tgagggtttg ttgaggctta atatgggctt
    10081 tatatcacat tgtggaggct tggtaaggca aaattacttg aggccgacct tgtaaacttc
    10141 gctaagaatc taagtgacag ttagcccaaa ctgaggtgtt atgatgcaga tatccgctct
    10201 gtttgctgtc ttttaaggga aggttatgaa gcctattgtg tatttgggag cggcgttggt
    10261 cagcagtatg atgctcagtg cttgctttac cagcgtgtat gccatcaatc ggttagaaaa
    10321 tgggagcttg cccgaaaccc agcgcaattt gaatgcttgg gcgcgaacaa atcccaatgc
    10381 ccatcgcagt gccagtttgc aagtgagtat cgaaggggat gctcccgctg ggttgcaagc
    10441 gctaccactg caattggatt atgtggcgga caattgtggc tttaaggggc gcagtgtcga
    10501 gggtttgctt gaagcgtatt atccccatca acgtgtgaat attcctgttt cagctgccac
    10561 acaccaaacc taccattatg tgtatcagcc agatgcgttt ttgccgcaaa aagatgagca
    10621 cggccgcact tgtgtgtggc gggtaacgca tgcgcaagtg caattgaaag ccatcaatcc
    10681 tgccagtaaa tctgtggcaa acattcaatt ttctttgttt gacaaggatt tgaatcaaac
    10741 acaaaccttg actaaatact ttggccgaca aacagagggt gatcaatttc ccaacatcac
    10801 cctaaaaagt gcccaggtac agcccgaaca agcttttagg gtgatggtgc ggcatcagcc
    10861 tgaaaacgat tgaagtgctt acaggccgct tgtgtacatt tacaagcggc ctgtaagaga
    10921 tgggttttga gtgtgatggg gcttaagaag cgtttatgtt ggcaggagta aatacaggca
    10981 attgggcact gggcacaaag aatgtgtcca accatgcttc gtccacttcg gccacagctt
    11041 tgacccagtg tgggtttttg tctttgtcta tgagcaaggc acgcacacct tcgagaaagt
    11101 ctgggcgggt gcagcactgt tgcgacacca ccaattcttg tgcgatgcac tcggccaaag
    11161 aggcgtgttt catgtcgcgt aacaagcgcc aaatcaagcc catgctggtt gggcagcctg
    11221 cttgggtgtt gacatgtgct tgtgccagcc acgcatcttg ctcgacatgc tcagccaata
    11281 cagccaaggc atcttgaaaa gtggcttggc ttaccacagc ttgtaccacg gtttgttgtg
    11341 ccatgatttg gctctcgggc aagtctgcaa cgtccaaacc attcaaaaca cgacgcacca
    11401 cggcttgggc attgtcttgc caatcggctg caaccaaggc ttgtaaggtg gcgacacggc
    11461 tgtcggcagg gcgagaggcc acggccacat tgccgagcag agcatcgctg ccattgaagc
    11521 gtgagcctgt taagcccaaa aatgtgccgc tgccaaacgg aaactgctgt aaaaaccaag
    11581 tgccgcctgc atcgggatac aggccgatgc tgatttcggg catggccatg acggtggttt
    11641 cggtgacgat ggtgtggctg gctgcactgg ccaaacccat accgccaccc atcacaatgc
    11701 cagtggccca acaaacaatg ggcttaggat agcggctgat gtattcacac aaatagtatt
    11761 cagcggcaaa gaaggtgtgc acatgttggt gagcggccgc ttcatcggcc gcttcgacca
    11821 cacccagtct gagctcacga atatcaccgc ctgcacaaaa agacttgtcg ccctcgccat
    11881 ccaaccacac cgtggccacg ctatcgtcat gttcccatgc ttccaattgc acacgcagcg
    11941 ctaaaatcaa ggcagtattt tgcgcattca aagattttgg gcgatttaat gttgcgatgg
    12001 caatgacttg gttgtggctg gtttgtttgg ttgaaaacaa gactaaaggc gtattcacgc
    12061 tgactccgat ggtggttgtg tgattcttga taattgttta atgaatatta tagcaaagaa
    12121 atggcgaaaa tttcatacgc caacgtattg aaaccactta cacttcgggt taaagtttct
    12181 gttcttaatt tatctttaca atgtggccag tagcttgttt caaccaagct tcacacattt
    12241 attcgggtat attcgaagcc tcaggccgct tacagaatca ttaatcggat gatgcacatc
    12301 atccacacca tcagtgaaag gggaagaaac gatgcaaatc ggcattccta aggaaagttc
    12361 gctcggtgaa ttgcgcgttg cagc
//
LOCUS       DAOMVK010000010        12018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_1223_length_12018_cov_3.315640, whole genome shotgun sequence.
ACCESSION   DAOMVK010000010 DAOMVK010000000
VERSION     DAOMVK010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 12018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12018
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1223_length_12018_cov_3.315640"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="PK856_00835"
     CDS             <1..572
                     /locus_tag="PK856_00835"
                     /inference="COORDINATES: protein motif:HMM:NF021572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2300 domain-containing protein"
                     /protein_id="HRL00947.1"
                     /translation="ACLLMRDTTQQQRVLTQAASAATKRIALSTDALVLRGAHGHFQA
                     ASSQKNVLGWQQAVAWAEQGLGFKAILARAYPQASIAVQGNDLGERCRPMPQAQKWLQ
                     TQSPRWHKVLQRETGYRPLQQAASVCQLSRGTPYADVASQRVYIRRLNSEAEQITLTH
                     EYLHLLFADHPNGRNERYIEALAQRLLKG"
     gene            578..1342
                     /locus_tag="PK856_00840"
     CDS             578..1342
                     /locus_tag="PK856_00840"
                     /inference="COORDINATES: protein motif:HMM:NF021430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2135 domain-containing protein"
                     /protein_id="HRL00948.1"
                     /translation="MQRLILASLSLLAISPVFAESGIEFSTPKGGWRHAQSQSQYTQS
                     VQYPASRVNSENFNKQSQLIAGKIKNHIKDSSKPARLIVNGIAMPQAINDDGSFSRPY
                     AFGRGSNSVELRTATGDKKRVQFYENNKAMTSPKLRVVLSWDSDNTDLDLHVVTPDGG
                     HSYYGNRVLNNGGALDVDVTTGYGPEIFSSPSPQNGTYLVYLNYYGGESDKVMTTATL
                     TIISNEGRANEKKQSFRVPMRAAGELTLVKSFHYRG"
     gene            1374..2105
                     /locus_tag="PK856_00845"
     CDS             1374..2105
                     /locus_tag="PK856_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00949.1"
                     /translation="MKLLLKISVIGLSMALLGACSSTNSPNASSARVAHKDERTLGYF
                     SERGVYQRDKIANGFERKLLGTTRKQHWVLQDFYSINGKPQTAPFTVIDERALYDWDT
                     MPFIDGPVQFYYPDGKKQSLITLKQGVVTGKREVYYLTGEPFQVQTFNEHGRITEETF
                     FERDGKRLFTIQYNPNNAEQSSLVYYDDTGKSHFASAENREQVKALADKVQAIMMELE
                     LEVYKQSGQTPPDNESALAEAPTTM"
     gene            2166..2915
                     /locus_tag="PK856_00850"
     CDS             2166..2915
                     /locus_tag="PK856_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00950.1"
                     /translation="MSFKHISLATLISLALLSGCATTAGHSAATEPENQVKIAQADER
                     VLGYFSADGEFGQEKSGNGYLRKYLGKTAEGKSVLQDFYANNGKPQTSPFVLFDDAGL
                     EDWNSLQYTDGNIVFYNADGKVSARAALNEGRYVGEHIQYHGNGQAFVKEQYNPESEI
                     TATTYYDEAGKPLFSFSIDSGNGDYKVDIKVYDAAGTAYPATEVHEDKIQAATDTIVA
                     LMNKRAALEAKLAGQDAPEPEALILPRMPEE"
     gene            3000..3995
                     /locus_tag="PK856_00855"
     CDS             3000..3995
                     /locus_tag="PK856_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00951.1"
                     /translation="MSIVLTRTLGSVLLAATLLACSHVQPLASQDSLTQTVSQTRTPK
                     LTRAPNLVQAHTSTVGYFNSEGQFSREQQADSAYVRKKLGQDRLGRGVFQDFYLWQER
                     PQTSLFALTDTSAETAWSNAEYVDGEFVFYDPTGRVSSHVNQKNGAYIGLQRHYHADG
                     SLFKLSRYDYFGNPLQTTYYRPDGKPIYQVTFDLANDDPRALVLFDAQSEPYPTEQLN
                     AAIVQQAEADIAAIINDLRIRTARLAHFTPPSAYAIKLPFKLDAVIAGQCQKHAIKQL
                     MGMQNLSNAQIKTISGANQVRRAADNEAVEDDFQASRVTVFLKPDTQHISAAFCG"
     gene            complement(4061..4777)
                     /locus_tag="PK856_00860"
     CDS             complement(4061..4777)
                     /locus_tag="PK856_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003791815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HRL00952.1"
                     /translation="MAKFLNTSATNYFLEELIKQAKERVILISPFLKLNDRVKELLED
                     KNRLKIDIRIVYGKSELQPEEIQWLKSQDYIRTSFCKNLHAKCYLNEDSCIITSLNLY
                     EFSQINNNEMGIFLSREDDGEVYKDAYEEAQRIIRVSDEVRISMEKIDEAEVGKATEV
                     KVAKAASKPQHEKLTTSKLATQLKLKTTELLEKMVQSGWLELKGDKHFITELGKQKGG
                     EFRTGPHGAYFLWPADTECQ"
     gene            complement(4937..5254)
                     /gene="sugE"
                     /locus_tag="PK856_00865"
     CDS             complement(4937..5254)
                     /gene="sugE"
                     /locus_tag="PK856_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013811228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HRL00953.1"
                     /translation="MAWIFLLVAGGLEVVWAYFMKESNGFSRLWPSVITIVAMIASFG
                     LLSAAMRTLPLGTAYTIWTGIGAVGAFIVGIVFLGEQISAMRIAAAVLIVSGLVMMKL
                     ASH"
     gene            complement(5420..6739)
                     /locus_tag="PK856_00870"
     CDS             complement(5420..6739)
                     /locus_tag="PK856_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL00954.1"
                     /translation="MDLVARVQRLPIGKFHYRLLVVVGLGWMFDAMDTGLIAFIMTRL
                     ATDWQLTPPEKGLIVSIGFVGMAIGAIVAGAIADKIGRKTVFASTLVIYSVATALCGI
                     APDLTWLLVFRFIVGIGLGGQLPVAVTLVSEYVPAHVRGRFIVLLESFWGLGWLAAAL
                     IAYFVIPNHDWHAAFFVGGIPALYVFLLWKLVPESIPYLINKGRLEEAHAIVSKLERD
                     AGVEVVEKIEVAPVAEKSTISFGQLWSKTFARRTLMLWLIWFGIVFSYYGIFTWLPSL
                     LVEKGYPIVKSFEYSLILILAQLPGYVAAAWLIEKLGRKTTLALFMAACAVCAYFFGG
                     AGSQTEIIVWGCLLSFFNLGAWGVLYTYTPEQYPTNIRAFGSGWASAVGRIGGIVAPM
                     VVTNMMVLPNGFSSVFTMFTVVLFAVAAVVFVLGEETKGKTLESISQ"
     gene            complement(6919..7869)
                     /locus_tag="PK856_00875"
     CDS             complement(6919..7869)
                     /locus_tag="PK856_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00955.1"
                     /translation="MQNTSPLNPNLQEHIQTAATELVAVLVAVTHGQARILTTHQGQS
                     LPAGVLSPLHRSLQKGVRVWVEEQTAQPLGYLEQLYTFVDTQRGQAAKQLWLSVSYVG
                     LVTEVAADHLNLQAKWMDWYAFFPWEDRRIVQTERDRVILSALTAWIAASSDVETQQF
                     RQQRVDMYWGQGEWSWSEELALQRYELMYETGMLQESPEANPALLPYTGMAMQGDHRR
                     IFATAVSRLRAKIKYRPLIFDLMPAEFTLLQLQQSIETLAGLTLHKPNFRRLIQHQDL
                     VEPTGSSVMQERGRPAQLYRFKPNVLKEQSLARGKMPLNK"
     gene            complement(7909..8814)
                     /locus_tag="PK856_00880"
     CDS             complement(7909..8814)
                     /locus_tag="PK856_00880"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRL00956.1"
                     /translation="MNQQQLGAVQMTTAMALSGGVGVWATWAALPSFAVVFWRCVFGF
                     IALLAFMLFTGRIHRGWITRSQMLWSLAAGTTLVLNWVCFFQSFHYVSIGVATIVYQA
                     QPLLVVVGGMVFFKERMGLNRWLWLGLGLMGMVLLVLARGGVSLSGDQYLLGIVLALA
                     AASFYAAGTLVAKHMPKTPATTVTLMQVLVGIGIMLPFGVWAHVPQTSVQWMSVTMLG
                     VVNTALMMSLVYAAISRLPTYVFAALSFVYPLVAVLLDWWVFGHMLNGWQIFATGLIL
                     LAVAGMNLNWTFSRKTKRPESPSRC"
     gene            8932..9375
                     /locus_tag="PK856_00885"
     CDS             8932..9375
                     /locus_tag="PK856_00885"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRL00957.1"
                     /translation="MLDKVDQKIIALLQQNARIAYKDLAVHVGLSAPAVTERIARLQE
                     RGVIQAYRTDIDYFACGYQLEAVWLVRTHHQQLFAAIDLVKSLPEVVSCDTVTGENCL
                     LVRLFAKNVQHLDQLTYRVLQYGEVNTMIVKQHIVSPRLPPLSPM"
     gene            complement(9416..10150)
                     /gene="pnuC"
                     /locus_tag="PK856_00890"
     CDS             complement(9416..10150)
                     /gene="pnuC"
                     /locus_tag="PK856_00890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HRL00958.1"
                     /translation="MMRLTTISIPPIGTHHDRHCAVFTGTLYTRTQIIIESLAAVFGV
                     VSVLFAQRRNIWIFPTGLISTVLYTYLFFRWGMYGETLINVYYSAMSVYGWILWANKA
                     EDHIHVQVAWAGKRDWLHASWVWVASFAFVLGVYYFRPWIQEGFGDAGWQQIGVHHFG
                     WVDYLDASLAAIFFIGMWFQAQRKIDSWTLWIIGDAVMVPLMLYKGYGITSLQYAIFV
                     VLAVQGMLAWIRSLRSEAQATPAAAV"
     gene            complement(10244..10612)
                     /locus_tag="PK856_00895"
     CDS             complement(10244..10612)
                     /locus_tag="PK856_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00959.1"
                     /translation="MDGYGVNEILMIIGVSILFCPSLLALPAWIWAAWRKPLMGNKKL
                     FLVGMGLHVLGILVAIGVFGWLKYVASDCHGCVSGLHLFLMLPITWLLFVCGAICNGM
                     GWRQPKPADGNGRRIKRRKT"
     gene            complement(10689..11066)
                     /locus_tag="PK856_00900"
     CDS             complement(10689..11066)
                     /locus_tag="PK856_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00960.1"
                     /translation="MFVVGMGVHVLGIIIAIGIFWWLKDTASNCRDCLSGLHLFLVLP
                     IIWLLFVCGATCNVVGLRQPSVADGKRSASKEEIPEYSLFGDAFYDKQRCRVSSQGML
                     LLSVALLVLLKPTIKIRDENVWV"
     gene            complement(11293..11772)
                     /locus_tag="PK856_00905"
     CDS             complement(11293..11772)
                     /locus_tag="PK856_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00961.1"
                     /translation="MSYHYVGKLSDANVFLPYIARAPALFVKSHHSAYPKARVWAAFA
                     FSPGVVGISVGVYIGIATADVGNLLSSAAVFAFFGQLLFGVPAALTGGVAAYFRWRKT
                     VKHVLASALVGGGLTWAIGFVVTKDVAVTTGCAAIGLMVALVVALWVLPKSTIKSKR"
BASE COUNT         3333 a         3379 c         2649 g         2657 t
ORIGIN      
        1 gcgcgtgttt gctgatgcgc gacaccaccc aacaacaacg cgtgctcacc caagccgcgt
       61 cagccgccac caaacgcatt gccttgagca ccgatgcctt ggtgttgcgc ggcgcacacg
      121 ggcattttca ggctgcatcc agccaaaaaa atgtgttggg ttggcaacaa gccgtggctt
      181 gggctgagca aggcttgggt tttaaagcga ttttggcgcg cgcttatccg caagcgagca
      241 tcgctgtaca aggcaacgat ttgggcgagc gctgccgccc catgccacaa gcacaaaagt
      301 ggttgcaaac ccaaagcccg cgttggcaca aggttttgca acgggaaacg ggttacaggc
      361 cgcttcaaca agcggccagc gtgtgccaac tgtcgcgcgg cacgccgtat gccgatgtgg
      421 caagccagcg ggtgtacatc cgccgtttga acagcgaggc cgagcaaatc acgctcaccc
      481 acgagtattt gcatttgctc tttgccgacc accccaatgg ccgcaacgaa cgctatattg
      541 aagcgctggc acagcgcttg ttgaaaggat aaacaccatg caacgcctga tactggccag
      601 cttgagccta ttggccataa gccctgtctt tgccgaaagc ggcattgagt tctccacccc
      661 caaaggcggt tggcgtcatg cacaaagcca aagccaatac acgcaatcgg tgcaataccc
      721 tgcttcgcgc gtgaacagtg aaaattttaa taagcaatcg caattgatcg caggcaagat
      781 taaaaaccac atcaaagaca gcagcaaacc cgcgcgcctg attgtgaacg gcattgccat
      841 gccgcaagcg attaacgacg atggcagctt cagccgcccg tatgcgtttg gtcgcggcag
      901 caacagcgtg gaattacgca ccgccacagg cgacaaaaag cgcgtgcagt tttatgaaaa
      961 caacaaggcg atgaccagcc cgaaattgcg cgtggtcttg tcttgggatt cggacaacac
     1021 cgatttggac ttgcatgtgg tgacacctga cggcggacac agttattatg gcaatcgggt
     1081 attgaacaat ggcggtgcgc tggatgtgga cgtgaccaca ggctacggcc ctgaaatctt
     1141 ctcctcccca tcgccacaaa acgggacata cctcgtgtat ttaaactatt atggcggtga
     1201 aagcgacaaa gtgatgacca cagccaccct caccatcatc agcaatgaag gccgtgccaa
     1261 tgagaaaaaa caaagcttcc gcgtgccgat gcgcgccgca ggcgagctga cattggtgaa
     1321 atcatttcat taccgtggtt aggccacgat aagggttaat caggaaaacg accatgaaac
     1381 tgctccttaa aatcagcgta attggcttga gcatggcctt attgggcgcg tgcagcagca
     1441 caaactctcc caacgcctct tctgccagag tcgcgcacaa agacgaacgc actttgggct
     1501 atttttcaga acgcggcgtc taccaacgcg acaaaattgc caatggtttc gagcgcaagc
     1561 tgttgggcac cacgcgcaaa cagcattggg tgttgcaaga cttttacagc atcaatggca
     1621 aaccgcaaac ggctcccttc actgtgattg atgagcgcgc cctttacgat tgggacacca
     1681 tgcctttcat cgatggcccc gtgcagtttt actatcccga tggcaaaaaa caatcgctaa
     1741 ttaccctcaa acaaggcgtg gtcacaggca agcgtgaggt gtattacctc acgggtgaac
     1801 ccttccaagt gcaaaccttt aatgaacatg gccgcatcac cgaagaaacc tttttcgagc
     1861 gcgatggcaa acgcctgttc accatacaat acaatcccaa caatgctgaa cagtcttcat
     1921 tggtgtatta cgatgacact ggcaaaagcc acttcgcctc agcggaaaac cgcgaacagg
     1981 taaaagcctt ggcggacaaa gttcaagcca tcatgatgga attggagctt gaggtttaca
     2041 aacaatcagg gcaaacacct cctgacaatg aatcggcctt ggccgaagca cccaccacca
     2101 tgtgagttga tgcggcctgt acacgtttac aggccgcatt tttttgagta ataagaggaa
     2161 aatccatgtc tttcaaacac atcagtttag ccaccctcat cagcttggcg ctgttaagcg
     2221 gctgtgccac cactgcaggc cattccgctg ccacagagcc tgaaaaccaa gtgaaaatcg
     2281 cccaagccga tgagcgtgta ttgggctatt tcagcgccga tggcgaattt ggccaagaaa
     2341 aaagcggcaa tggttatttg cgcaaatact tgggcaaaac cgccgaaggc aaatcggtgt
     2401 tacaagactt ttacgcgaat aatggcaagc ctcagaccag cccatttgtg ttgtttgatg
     2461 atgcaggttt ggaagattgg aacagcttgc aatacaccga tggcaacatc gtgttttaca
     2521 acgccgatgg caaggtttct gcccgtgccg ccttgaatga aggccgctat gttggtgaac
     2581 acattcaata ccatggcaat ggccaagcct tcgtgaaaga gcaatacaac ccagaaagtg
     2641 aaatcacggc caccacttat tacgatgagg cgggcaaacc cttgttctca ttcagcatag
     2701 acagcggtaa cggcgattac aaagtggaca tcaaagtcta cgatgcagca ggtaccgcct
     2761 acccagccac cgaagtccac gaagacaaaa ttcaggccgc cacagacacc atcgtggcct
     2821 tgatgaacaa acgtgcagcc ttggaagcga aattggcagg ccaagatgcg ccagaacccg
     2881 aagcattgat cttgccacgc atgcctgaag aataaatccg ccctcacatg cggcctgtaa
     2941 acccccaaca gcgtttacag gccgcttttg ccataaaaga cagtcacgga gagcaaacca
     3001 tgtctattgt actgacccgc acattgggga gtgtcttgtt ggcggcaacc ttgctcgcct
     3061 gcagccatgt ccagcctcta gcgtcccaag actcgttaac gcaaaccgtc agccaaaccc
     3121 gcacgcccaa actcacccga gcaccaaatt tggtgcaagc ccacaccagc accgtgggct
     3181 atttcaacag cgaaggccag tttagccgcg agcaacaggc cgacagcgct tatgtgcgca
     3241 aaaaattggg ccaagaccgc ttgggccgcg gcgtgtttca agatttttac ctgtggcaag
     3301 aacgcccgca aaccagtctg tttgcactga ccgacacctc ggcagaaacg gcatggagca
     3361 atgcggaata tgtcgatggt gaatttgtgt tctatgaccc cacaggacga gtgtcttccc
     3421 atgtgaacca aaaaaatggt gcatacatcg gtttacagcg gcactaccat gccgatggca
     3481 gcttgttcaa actcagccgc tacgattatt ttggcaaccc actgcaaacc acgtattacc
     3541 gccccgatgg caaacccatc taccaagtca catttgattt ggccaatgat gacccgcgtg
     3601 cattggtatt gtttgatgca caaagtgagc cctatcctac cgaacaattg aatgccgcca
     3661 tcgtgcaaca agccgaagct gatattgcgg ccatcatcaa tgatttaaga atccgcaccg
     3721 cccgcctcgc tcacttcacg ccaccatcag cctacgccat caagctgcca ttcaaactcg
     3781 atgccgtcat cgcaggccag tgtcaaaaac acgccattaa acaactcatg ggcatgcaaa
     3841 acctcagcaa tgcccaaatc aaaaccatca gcggcgcaaa ccaagtgcgc cgcgccgccg
     3901 acaatgaagc ggtagaagat gatttccaag ccagccgtgt caccgtgttt ttaaaacccg
     3961 acacccagca catcagcgca gcattttgtg gctaatgcag aaaaaagcgg cctgtaagct
     4021 tcaatgtgag cttactggcc gcttatcgat tctaacttgt ttattgacac tcagtgtcgg
     4081 caggccatag aaaataagca ccgtgtgggc ccgtacggaa ttcgccacct ttttgtttgc
     4141 ccaactcagt gatgaaatgc ttgtcgcctt tcagttctag ccaaccgctt tgcaccatct
     4201 tttccaacaa ttcagttgtt tttaatttga gttgagtcgc caatttggat gtggtgagtt
     4261 tctcatgttg cggcttgctg gctgctttag ctactttgac ttctgtggcc ttgcccactt
     4321 ctgcttcgtc aattttttcc atgctgatgc gcacttcatc actcacacgg atgatgcgct
     4381 gcgcttcttc atacgcatct ttgtaaacct caccatcgtc ttcgcggctt aaaaagatgc
     4441 ccatttcatt gttgttgatt tggctgaact catacaaatt cagactggtg atgatgcaac
     4501 tgtcttcatt caaatagcat ttggcatgca ggtttttaca gaaactggtg cggatgtaat
     4561 cttgtgattt caaccactgg atttcttcgg gttgtaattc gcttttgcca tacacaatgc
     4621 ggatgtcaat ttttaagcgg tttttgtctt ccaacaattc tttcactcgg tcgttaagct
     4681 tcaaaaatgg gctaatcaaa atcacccgct cttttgcctg ttttatcaac tcttctaaaa
     4741 aataattggt cgcactggtg ttcagaaact ttgccatatc tccccctgtt aaatatgcaa
     4801 ataaagtaaa tacagctcat tctagattaa ataaagcggc ctgtaagctt tacaggccgc
     4861 ttttaaagaa gcagcaaatc aaccattgac acagcttgcg ctgacaagcc attggtggtg
     4921 gcgcaattga atgtgattaa tgcgaagcca atttcatcat taccaaaccg ctgacaatca
     4981 acacagccgc ggcgatgcgc atggcactga tttgttcacc caaaaacacg atgccaacga
     5041 tgaaagcgcc aaccgcgcct atgcccgtcc aaattgtgta agccgttcct aaaggcaagg
     5101 tacgcatggc ggcagacaac aagccaaaac tggcgatcat cgccacaatc gtaatcacgc
     5161 tcggccacag gcggctaaaa ccgttcgact ccttcatgaa atacgcccaa accacttcca
     5221 aaccacccgc taccaataaa aaaatccagg ccatcttgac cctcctttag ttgagagcca
     5281 ggtcgtcctg gacattgttc cctgatttgg gggaggccgt tcctccttaa gtggcttgca
     5341 tcatagcaaa ctgatgcaag ccgtcaagtc tgtgtggcct ttgttacaca ctgttacctg
     5401 ttgttacctt acaggccgct tattgactga tggattcgag ggttttgcct ttggtttctt
     5461 cacctaacac aaacaccacc gccgccaccg caaacaacac cacggtaaac atcgtgaaca
     5521 cgctgctgaa gccattgggc agcaccatca tattggtcac caccatcggt gccactatgc
     5581 cgccgatgcg acccactgcc gaggcccaac ctgagccaaa cgcacggatg ttggttgggt
     5641 attgttctgg ggtgtaggtg taaagcacgc cccatgcacc caaattaaag aacgacaaca
     5701 aacagcccca gacaatgatt tcagtttgcg agcctgcgcc gccaaagaag taagcacaca
     5761 cggcacatgc ggccataaac aaagccaacg tggttttgcg acccagtttt tcaatcaacc
     5821 atgccgccgc cacatagcct ggcaattgcg ccaaaatcag aatcaagctg tattcaaacg
     5881 atttcacgat gggatagcct ttttccacca acaggctggg caaccacgtg aagatgccgt
     5941 aatacgaaaa cacgatgcca aaccaaatca accacaacat caaggtgcgg cgggcgaatg
     6001 tcttagacca caattgccca aagctgatgg tggatttttc cgccacaggc gcgacttcga
     6061 ttttctccac cacttctacg cctgcatcac gctccaattt cgacacgatg gcatgcgctt
     6121 cttccaagcg gcctttgtta atcaaatatg ggatggattc aggcaccagt ttccacaaca
     6181 aaaacacata caaagcagga ataccgccca caaaaaaggc cgcgtgccaa tcgtggtttg
     6241 gtattacaaa ataggcaata agagcggcag ccaaccaacc caagccccaa aaactctcta
     6301 acaacacaat gaagcgccca cgcacatgtg ctggcacgta ttcactcacc aatgtcaccg
     6361 ccacaggcaa ctgcccaccc aagcctatgc ccacgatgaa gcgaaacacc aacagccaag
     6421 tcaaatcagg cgcgatgcca cacaaagcgg tggcgacgct gtaaatgacc aaggttgagg
     6481 caaacacggt tttgcggcca attttgtcgg ctatcgcccc tgccacgatg gcacctatcg
     6541 ccatgcccac aaagcctatg ctgactatca agcctttttc aggcggcgtc aattgccaat
     6601 cggtggccaa acgcgtcatg atgaacgcaa tcaagcccgt gtccatggca tcgaacatcc
     6661 atcccaaacc caccaccacc aataagcgat aatgaaactt gcctatcggt aaacgctgca
     6721 cgcgtgcgac caaatccatt gttgtgtttc cctgtgtgtc gtcaaaagaa ccaagctcag
     6781 actccttttt ctgcgcttgg tttacatgcc gaaaatatat actcattttg agcatatatc
     6841 aattaaaaaa tacagcgaca gagaaatcgc tgcatctcgg gctcgatgtg gtcagaaact
     6901 gctgcgccac agacgtgctt atttgttcaa gggcatcttg ccgcgcgcca aactttgttc
     6961 tttgagcaca ttgggcttaa agcggtaaag ctgggcgggt cgaccacgtt cttgcatcac
     7021 gctggagcct gtgggttcaa ccaaatcttg gtgctggatt aagcggcgaa aattgggctt
     7081 gtgtaaggtc aaacctgcca aggtttcaat gctttgttgt aattgcaata aggtgaattc
     7141 ggcgggcatc aaatcaaaaa taagcggcct gtatttgatt ttggcgcgca agcggctgac
     7201 ggcagtggca aaaatgcggc ggtgatctcc ttgcatggcc atgcctgtgt acggcaataa
     7261 ggcaggattg gcctctggcg actcttgcaa catgcctgtt tcatacatca attcatagcg
     7321 ttgcaaggcc aattcttccg accaagacca ctcgccttgg ccccaataca tatccacgcg
     7381 ctgttggcga aattgttggg tttccacatc gctactggcg gcaatccaag cggtcaaagc
     7441 gcttagaatg actcgatctc gctcagtttg cacgatgcgg cggtcttccc aagggaaaaa
     7501 cgcataccaa tccatccatt tggcctgcaa attcaaatga tccgcagcca cttcggtgac
     7561 caagcccaca taactgaccg acaaccacaa ttgtttggca gcttgaccgc gttgggtatc
     7621 gacaaacgtg tacaactgct ccaaatagcc caaaggctgg gcggtttgct cttccaccca
     7681 cacccgcacc cctttttgca aagaacggtg caagggtgac aacacgcccg caggcagact
     7741 ttgaccttgg tgggtggtca aaatccgtgc ttggccatgg gtaaccgcca ccaaaaccgc
     7801 caccaattcg gtagcggcag tttggatgtg ttcttgcaaa ttgggattca gagggcttgt
     7861 attttgcatg tcggtgtatc aaatccagaa tggcttaagc ataccatgtc agcaacggct
     7921 aggggattca ggccgcttgg ttttgcggga aaaagtccaa ttcaaattca tgcccgccac
     7981 cgccaacaaa atcaaacctg ttgcaaaaat ctgccaacca ttcaacatat gaccaaacac
     8041 ccaccaatcg agcaacaccg ccaccaatgg atacacaaac gaaagcgcgg caaacacata
     8101 tgtgggcaag cggctgatgg cggcatacac cagcgacatc atcaaagcgg tattgaccac
     8161 accgagcatg gtcacgctca tccattgcac cgatgtttgt ggcacatgag cccacacgcc
     8221 aaacggcaac atgataccaa tccccaccaa tacctgcatc aaggtcacag tggtagcagg
     8281 cgttttgggc atgtgtttgg caaccaaagt gcccgccgcg taaaacgagg ccgccgccaa
     8341 agccaataca atgccaagca aatactggtc accactcaga ctgacaccgc ctcttgccaa
     8401 caccagcaac accatgccca tcaaacccaa acccaaccac agccaacggt tcaggcccat
     8461 gcgctctttg aaaaacacca taccgcccac cacgactaac aatggctgtg cttggtacac
     8521 aatggtggcc accccgatgg agacgtaatg aaagctttgg aagaaacaca cccaattcaa
     8581 caccaaagtg gtgcccgcag ccaaagacca gagcatttgc gagcgggtaa tccaacccct
     8641 atgtatgcgg ccggtaaaca acatgaaggc caataaggca ataaagccaa acacgcaacg
     8701 ccaaaacacc actgcaaaac tcggtagtgc cgcccatgtt gcccacacgc ccaccccgcc
     8761 cgataaggcc atggccgtgg tcatttgtac cgccccaagc tgttgttgat tcatctcgtg
     8821 ctcctttttt aatgccttca gtatcgtgaa tttctgccgc cgccactatg gagggaacac
     8881 ggaatttctg ctatcttgct tgggaaaata aagaatttac aggacgctgc tttgttagat
     8941 aaggttgacc aaaaaatcat cgcgctattg cagcaaaatg cccgcattgc ctacaaagat
     9001 ttggccgtcc atgtgggctt gtccgcaccc gcggtaaccg agcgcatcgc ccgtttacaa
     9061 gagcgcggcg tgatacaggc ttaccgcacc gacatcgatt attttgcctg cggctaccaa
     9121 ttggaagcgg tttggttggt acgcacccac catcagcaat tgtttgcagc cattgatttg
     9181 gtcaaaagct tgcccgaggt ggtgagctgt gacacggtga cgggggagaa ctgcttgttg
     9241 gtgcgcttgt ttgccaaaaa tgtacaacac ctagaccaac tcacgtatcg ggttttgcaa
     9301 tatggcgaag tcaataccat gatagtcaag caacacatcg tcagcccccg tcttccccct
     9361 ttaagcccaa tgtaaaaaca gcatggttac catgctgttt tggtttacag gccgcttaaa
     9421 ctgcggcggc tggagtggct tgtgcctctg agcgcaagga gcgtatccat gccaacatgc
     9481 cttgcactgc caacaccaca aatatcgcgt attgcaatga ggtaatgcca tagcctttgt
     9541 acagcatcaa tggcaccatc accgcatcac caataatcca cagtgtccaa gaatcaattt
     9601 tccgctgcgc ctgaaaccac ataccgataa agaagatggc cgccaaactg gcatccaaat
     9661 aatctaccca accaaaatgg tgcacaccga tttgctgcca acctgcatca ccaaagcctt
     9721 cttgtatcca cggcctgaaa taatacaccc cgagaacaaa ggcgaaactc gctacccaca
     9781 cccatgaggc atgcagccaa tcccgctttc ctgcccaagc cacttgcaca tgaatgtggt
     9841 cttccgcttt gttggcccac aatatccaac cataaaccga catggccgag taatacacat
     9901 tgatgagggt ttcaccatac atgccccagc ggaaaaacaa ataggtgtag agcacagttg
     9961 aaatcaagcc tgtggggaaa atccagatat tgcgtcgctg cgcaaacaac accgacacca
    10021 ccccaaatac ggccgccaaa ctctcaatga tgatttgagt gcgggtgtat aaggtgccag
    10081 taaatactgc acaatgtctg tcatggtggg ttccaatggg tggtatcgaa atggtggtta
    10141 agcgcatcat tttatggcgc gatgtgcact atataccgat atctcagcca ttccaagcag
    10201 acaataaaac aagttttgct ccacgttacg aagaagacaa tgctcaggtt tttcttcttt
    10261 taatgcgcct gccgttccca tctgcaggct tgggctgacg ccaacccatc ccattgcaga
    10321 tggccccgca tacaaacagc aaccaagtga tgggcagcat caaaaacaaa tgcaatccac
    10381 tgacacaacc gtgacagtcg ctcgccacat actttaacca cccgaaaacc cctatggcaa
    10441 cgagaatacc caaaacatgt aaccccatgc ccacgagaaa tagtttttta ttacccatta
    10501 atggcttacg ccaagcagcc caaatccacg ctggcaaagc cagtagcgat gggcaaaaca
    10561 atattgaaac gccaataatc atgagtatct cattgacacc atatccgtcc atctagccct
    10621 ctctttcagc acacactcat gcgcattaat acctgttgag acaccatcat agcgatggtc
    10681 tgacttgctt atacccaaac attttcatct cttattttga ttgtgggctt gagcaatacc
    10741 aataacgcta cggacaataa aagcatcccc tgtgagctca ctctacatcg ctgcttatca
    10801 taaaaggcat caccaaagag actatactca ggtatttctt cttttgaggc gcttcttttc
    10861 ccatcggcaa cgcttggctg acgcaacccc accacattgc aggtggcgcc acacacaaac
    10921 aatagccaaa taatgggcaa caccaaaaac aaatgtagtc cactaagaca atcgcgacaa
    10981 ttacttgcag tatcctttaa ccaccaaaat atccctatgg caatgatgat gcctaaaaca
    11041 tgcactccca tgcccacgac aaacactctt ttatttctca tcgatggctt gcgccgccat
    11101 gcccacaacc acacagggaa agccagtaat gacgggaaaa atcacaatga aaatccaaac
    11161 atcatgaata tttcacctaa atcatatccg cccattttgc cctctctttc aatgtactca
    11221 ttcgcacatt tgcatcaatg cctgttgaga caacatcata gcctcactct aacttgctta
    11281 cacccaagca ttttatctct tgcttttgat tgtggacttg ggcaataccc acaatgccac
    11341 gaccaaagcc accatcaaac caatggcggc acaccctgtg gtcactgcca catctttggt
    11401 caccacaaaa ccgattgccc aggtcaaacc gcctcccacc aacgccgatg ccagcacgtg
    11461 ttttactgtt ttacgccaac gaaaatatgc cgccacccct ccagtgagcg cagcaggcac
    11521 accaaacaac agttgtccaa agaaagcgaa cacagccgcg ctgctgagca aattaccaac
    11581 atcagcagtg gcaatgccaa tatacacacc caccgaaata cccactacac ctgggctgaa
    11641 tgcaaaagcg gcccataccc gcgccttggg atatgccgaa tgatgcgatt taacaaataa
    11701 cgctggcgca cgagcaatat aaggcaaaaa cacattggca tcggacagct tacccacata
    11761 atggtaagac atcgcacact cctattgatg tagtgcagcc actgtaaaca caacttcagc
    11821 ccgctgccaa tccttgagta gcgggttagc aaaatgctgc tgacagtagc catttgttcc
    11881 caatgtttgt atgcaacaat tgagcacaaa aaagcggcct gtaaatgtac aggccgcttg
    11941 ttcatgcttt aaaccagatt actgttgttg caaacgcgca tgcaattctt gcacgctgta
    12001 cacatcggtg tcgttcaa
//
LOCUS       DAOMVK010000011        11857 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_1258_length_11857_cov_3.134130, whole genome shotgun sequence.
ACCESSION   DAOMVK010000011 DAOMVK010000000
VERSION     DAOMVK010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 11857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11857
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1258_length_11857_cov_3.134130"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="PK856_00910"
     CDS             <1..478
                     /locus_tag="PK856_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00962.1"
                     /translation="DWAPTTKTPTLWAWQVQPSAEISEFVFRLDYPVAWWQRVLVAIA
                     PWMWVVCLLLPLPFLCRHLRRLQRAGRKRARVVTLWAAVVLLPLLVLIMYALLVPLLM
                     QWGLGAVLSPSYGDAWVYMGMALLVLAYPATILVYALVLLAFGRVATPRTPSPPVV"
     gene            713..1546
                     /locus_tag="PK856_00915"
     CDS             713..1546
                     /locus_tag="PK856_00915"
                     /inference="COORDINATES: protein motif:HMM:NF020968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HRL00963.1"
                     /translation="MTLLKYPRTPHLQGSGLAPDDKETVPYSRLTGRLIVVEEKLDGA
                     NVGISFEQGGLHVQSRGHYLNLEQSGGRERQFNYLKLWAKTHETTLYALLGERYVMYG
                     EWLYAKHSVFYDALPHWLCEFDVYDREAACFLDTAARMALLADVPIVSVPVLYQGTAP
                     KSLKGLQALVQPSLAKSANWKNSFATACQRAQLDEALCWQQTDHADVSEGLYIKVEEN
                     GQVVERYKWVRSGFVQTIVDSGSHHSERPIVVNALREDLDIYAPEINKQWLQAACGGE
                     A"
     gene            1543..2688
                     /locus_tag="PK856_00920"
     CDS             1543..2688
                     /locus_tag="PK856_00920"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRL00964.1"
                     /translation="MKTNITAQIDALWQGQGVDFAACLDMFPVLEQAKTTPQDPVYHG
                     EGDVWTHTQMVVSELLALADFQAASESEQRVLFLAALLHDVAKYRTTVINEQGRISQP
                     GHSKKGALDARILLWELGLPFAEREAVCNLIAVHQVPFFAFEDNRHGHTPQWLCHSLS
                     WQTNIRLLCALAEADMHGRVCADKSQALDNIALLRELAREEGCEQTPKAFANEHTRLR
                     YFQGHEVYPDFALQMPQGSKVTLMCGLPASGKNTWVAQHAGGMPVLSYDDTRQRLGLK
                     YGANEGLVAHTVLEEAKAHLRAKQDFVWNATHLSVQMRQKNLAACLAYDAHVRIVCVE
                     ADKATLLKRNQARNSSLSNAKLLQMLKHWEMPTLFEAHQLKVSLNGL"
     gene            2922..3116
                     /locus_tag="PK856_00925"
     CDS             2922..3116
                     /locus_tag="PK856_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00965.1"
                     /translation="MLSVAELRQRKQQLEQTIAELIQVFEHETSVEVDSLLTVERCSV
                     NTPMQDAPTFHIKVDVHLNM"
     gene            3384..3647
                     /locus_tag="PK856_00930"
     CDS             3384..3647
                     /locus_tag="PK856_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00966.1"
                     /translation="MSLVQPFRPTAMQKPRLTPMPNLHSMGNRQAHLVQLFAARIALR
                     QRELSSGFLVFTDTGEVKYYAEHHSFAVVHLAAFTNMPVYSIK"
     gene            3867..5765
                     /gene="htpG"
                     /locus_tag="PK856_00935"
     CDS             3867..5765
                     /gene="htpG"
                     /locus_tag="PK856_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HRL00967.1"
                     /translation="MKETLSFQTEVKQLLQLMIHSLYSNKEIFLRELISNAADATDKL
                     RFEALNNAALLENDGDLQITVTCDTAAKTITITDNGIGMSRDEVIENLGTIAKSGTKA
                     FFEQLSGDAKKDANLIGQFGVGFYSAFIVADEVEVETRRAGSAANEAVRWVSKGDGEF
                     SIENIDMPERGTKITLHIKEDDADLLSDWTLRRIVTTYSDHISVPILMKKAPEYDDEG
                     NITHSDELEAVNQAQALWTRNKNDISDEQYQEFYKHISHDFSDALAWTHFRVEGKHEY
                     TGLMYVPKNAPFDMYDREPKGGVKLYVKRVFIMDDVQKLMPMYLRFIKGIIDTNDLPL
                     NVSREILQQSRDLDQIRAGAVKKILSMLEDIAKNRAEDYTQFWNTFGSVLKEGIGEDV
                     ANKDKIAGLSRFASTHNADAEQTVSLADYIGRMKEGQDKIYYITAETYAAAKNSPHLE
                     IYRKKGIEVLLLTDRIDEWVVGSLFEFDGKKLASVSKGELDLGSLENEADKAEQEKAQ
                     EAAKDVVAKIKADLGDKVADVRATTRLTESPACLVVGEHDMSQNLQRMLAAAGAADQL
                     PGAKPVLEINPEHMLVQRLASESNGVVQAELSQLLYDQALLAEGGKLEDPASFVKRMN
                     QLLVAAHA"
     gene            complement(5869..6399)
                     /locus_tag="PK856_00940"
     CDS             complement(5869..6399)
                     /locus_tag="PK856_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00968.1"
                     /translation="MMFRPFCLLLLATVPFTALADTKANTSEKNAPWAKNPRTQQFNP
                     VYTQQWRKAENRKTCALLVLPRDASAHLPQAKSRSASFSGGWAVAYDIPGKRSAYGVA
                     GTGANISNADIQRWENHRFWRDGSGIGWGLEGDTGPDYLAYVKVKGQKCLYNVWSNVS
                     EEHLLSLIQDLRQARP"
     gene            6534..7208
                     /locus_tag="PK856_00945"
     CDS             6534..7208
                     /locus_tag="PK856_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HRL00969.1"
                     /translation="MAVLSVQPINLLEVDSLYQQVQAELPDSIRLRVHRAMSWLKQAQ
                     QSDTEDMRFMCLWVAFNAAYARDLQVRTGGGDLVGTRDFLLKMCRFQPKVLTHLVWDI
                     YPQGIRQLLENRYVFQAFWDHHNGMLSEEAWQASFNKAKRKGLEALKNKDTADILMMV
                     YERIYTLRNQIFHGGATFGSKVNRLQLKSALGILADTIPVFLVVMLKHPNETVWGRPY
                     YPLVKD"
     assembly_gap    7372..7381
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7382..>7659)
                     /locus_tag="PK856_00950"
     CDS             complement(<7382..>7659)
                     /locus_tag="PK856_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRL00970.1"
                     /translation="LSKHALKSCTVLQHGSLQNFPAIRAELGNGRIVYAIHPPPPISA
                     YMATERDRVLQTIAKQVSHEQGEVLVMGDMNNTAFAPSFRRFTQISSL"
     assembly_gap    7660..7669
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7670..8132)
                     /locus_tag="PK856_00955"
     CDS             complement(<7670..8132)
                     /locus_tag="PK856_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00971.1"
                     /translation="MNSSRLLTFVRKRTVFLAYASLVAVILGELGRFYWLLELFSHFV
                     PYYAATLLLATLFSPKTWQKCWFGLSAVLLAIWMFTPLANPPTPAPTQQRFISYNLYI
                     NNLQVETDLNWLERQSADVVFLTEAAPSMQIVQRLQAAYPHGCERLDDSPFG"
     gene            complement(8136..8912)
                     /locus_tag="PK856_00960"
     CDS             complement(8136..8912)
                     /locus_tag="PK856_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRL00972.1"
                     /translation="MSTLTLTVLGCGSSSGTPVINCDCDTCLSGLPENQRSRCAAWLH
                     SDDGASIVIDTGTDFRTQALRERIPHVDAVLYTHPHADHLNGIDDLRAFCYQQKAAIP
                     VYGNGFTLANIQSRFDYAFLEPRDFWDKPVLKAHGLQAAQTIAGLPVQAFTVPHGRWS
                     VSAFRIGNIAWLTDLNDISDEVIAQLQGLDYLFLDCLMEHDYPSHLSVERAFAFAQRI
                     AAKQTYLIHMTHRLGYHELQARCPAGIAVAYDGLSVTSVL"
     gene            complement(8909..9688)
                     /locus_tag="PK856_00965"
     CDS             complement(8909..9688)
                     /locus_tag="PK856_00965"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003782618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRL00973.1"
                     /translation="MLIDSHCHLNFPDLVARLPEVLANMHSNDIRQALAISVSRDSYA
                     QVQQLANAHENIYCTIGVHPDEENAAEFSLEELLQAASLPKVVGIGETGLDYHWCQGD
                     LTWQHNRFRTHIQASNASGLPLIVHTRDAGADTLRLLSEEQAQAGVIHCFTEDVDFAR
                     QALDLGFYISFSGILTFKNAQQIQEAAKFVPQDRILVETDAPFLAPVPKRGKPNEPAY
                     VKYTAEFLAQLRGESFAEVEHYTCENFYRLFNKVPRIEANA"
     gene            complement(9693..10046)
                     /locus_tag="PK856_00970"
     CDS             complement(9693..10046)
                     /locus_tag="PK856_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRL00974.1"
                     /translation="MAQPQVPGKMVQVALADRTSIYNSYMSFLNNGGVFIASDDEFHM
                     GDEVLLVLEVGEAADRFPLKTTVCWINPIRGSSTRPRGVGLAFDASDTAKQARHYIEN
                     SLAGLLDLPRPTYTI"
     gene            complement(10048..11025)
                     /gene="holB"
                     /locus_tag="PK856_00975"
     CDS             complement(10048..11025)
                     /gene="holB"
                     /locus_tag="PK856_00975"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HRL00975.1"
                     /translation="MIYPWQQTVWQQATQRWQALPHAILLSGQQGTGKMVFAEHLAQA
                     LLCKHIDADKNACGMCSACHLFKQHSHPDCMVMRPETEEGNARKLQQIKVDAVREVLA
                     FAQLSSHQGGRRVIIIEPAEALNLQAANALLKVLEEPPANVVFILVSHHSERLLPTIL
                     SRLQVWHLPTPSTDVALNFLQQQGITLSKSQLAFQAGAPLLQEEGSLQDLRAQWLAIL
                     AKPRLLALLDFATEFDKHKRPLGEALDWLYKWLVDVGLARQGLPALYYPEQQSGLNAL
                     AQHSPQLWQQLVDKMHQLMPFGQHTLNVKLQLEDLSIDYLNLMTHKEAK"
     gene            complement(11028..11690)
                     /gene="tmk"
                     /locus_tag="PK856_00980"
     CDS             complement(11028..11690)
                     /gene="tmk"
                     /locus_tag="PK856_00980"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRL00976.1"
                     /translation="MMPSTNTSANAERGVFISLDGIDGAGKTTHLQAIQAWMTQQNLP
                     FVMTREPGGTPLGESLRALLLDPATHISNETECLLMFAARQQHLQDVIEPALAQGTHV
                     ISDRFTDATFAYQGGGRGLPWQKIATLELWVQAGLQPDLTLLLDVPLEVSMGRIEKSR
                     EKDRFEQEQAEFFTRVRNAYQQRAQAAPERYALINAHRERELVSADVLAAVARVFAAE
                     GA"
     gene            complement(11656..>11857)
                     /locus_tag="PK856_00985"
     CDS             complement(11656..>11857)
                     /locus_tag="PK856_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRL00977.1"
                     /translation="YNTYVIDGLPPTPISLPSTAALEAAAHPSAEKYLYFVSKMDSTG
                     ESYFSHDLKEHNDAVNKYIRKR"
BASE COUNT         2910 a         2739 c         3191 g         2997 t
ORIGIN      
        1 cgattgggct cccaccacta aaacgcccac attgtgggcg tggcaagtgc agcccagtgc
       61 cgaaatttcc gaatttgtgt ttcgcctaga ttatccagtg gcgtggtggc aacgggtgtt
      121 ggtggcgatt gcaccgtgga tgtgggtggt ctgtttgctg ttgcctttgc cgtttttgtg
      181 tcgccatttg cggcgtttgc agcgcgcagg gcgtaagcgc gctcgagttg ttaccttgtg
      241 ggcagcggtg gtgttgctgc ctttgctggt gctcatcatg tatgcgctgt tggtgccgct
      301 gctgatgcaa tggggcttgg gtgctgtgtt gtcacccagc tatggcgatg cttgggtgta
      361 tatgggcatg gccttgctgg tattggctta tcctgccacc atcttggtgt atgccttggt
      421 attgctggct tttgggcgag ttgccacgcc acgcacccca tcgccgcctg tggtatgaag
      481 tgcacacaag cggcctgtaa acacggttag ggttttacag gccgctatta gagcctctat
      541 actccgctcc cattacttca acccctattt ggatttctat cctatgccac gtttaaacat
      601 tcaaccgaat taaacgacgc attctttttt ggatgcgtgt gtgtctgcac catcctctca
      661 tccgcagtgc ccactttatc gctttggttc ggttacacga aagaaccaaa ccatgacttt
      721 attaaaatac ccgcgcacgc cgcatttgca aggctcaggc ctcgcgccag acgacaaaga
      781 aaccgtgcct tattcaaggc ttacaggtcg cttgattgtg gtcgaagaga agctagacgg
      841 agccaatgtc ggcatcagtt ttgagcaagg cgggttgcat gtgcaatcgc gtggccatta
      901 tttgaacctc gaacaatcgg gcgggcgcga gcgccaattc aattatttaa aactgtgggc
      961 gaaaacccat gaaaccactt tgtatgcgct tttgggcgag cgctatgtga tgtatggcga
     1021 gtggttgtat gccaaacatt cggtgtttta cgatgcctta ccgcattggc tgtgcgaatt
     1081 tgatgtctac gaccgcgaag ccgcctgttt tctcgatacc gccgcgcgca tggcattgct
     1141 tgccgatgtg cctattgtgt ctgtgcccgt gttataccaa ggcacagcgc ccaaaagcct
     1201 gaaaggctta caagccttgg tgcaaccttc tttggcgaaa agcgccaatt ggaaaaacag
     1261 ttttgccacc gcttgtcagc gcgcgcaatt ggatgaagcc ttgtgttggc aacaaaccga
     1321 ccacgccgat gtgtctgaag gcttgtacat caaagtagaa gaaaacggcc aagtagtgga
     1381 gcgttacaag tgggtgcgct caggctttgt gcaaaccatc gtggattcgg gttcgcacca
     1441 cagcgaacgc ccgatagtgg tcaatgcctt gcgcgaggac ttggacattt acgcgcctga
     1501 aatcaacaag cagtggttac aggccgcttg tggaggtgag gcatgaaaac caatatcaca
     1561 gcccaaatcg atgcgctttg gcagggacaa ggtgtggatt ttgccgcctg tttggacatg
     1621 tttcctgtgt tggagcaggc caaaaccacg ccacaagacc ctgtgtacca cggcgagggc
     1681 gatgtgtgga cgcacacgca aatggtggtg agcgaattgt tggcattggc cgattttcag
     1741 gccgcatctg agtcggaaca acgggtgttg tttttggcag cgttgctgca cgatgtggcc
     1801 aaataccgca ccaccgtcat caatgagcaa ggtcgcatct cacagccagg acattccaaa
     1861 aaaggggctt tggatgcgcg catcttgttg tgggagttgg gcttgccatt tgctgaacgt
     1921 gaggctgtgt gtaacttaat cgccgtgcac caagtgccat ttttcgcctt tgaagacaac
     1981 cgtcatggtc acacaccgca atggttgtgc cacagcctgt cttggcaaac caacatccgt
     2041 ttgctgtgtg ccttggctga ggccgacatg cacgggcgag tgtgtgcgga taaaagccaa
     2101 gccttggaca acattgcctt gctgcgagaa ttggcgcgag aagagggttg tgagcaaacg
     2161 ccaaaagcct ttgccaatga acacacgcgc ttgcgctatt tccaagggca tgaggtgtat
     2221 cctgattttg ccttgcaaat gccacaaggc agtaaggtaa cgctaatgtg tggcttgccc
     2281 gccagtggca aaaacacctg ggtggcgcag catgcaggcg gtatgcccgt attatcatac
     2341 gatgacaccc gccaacgctt gggcttgaaa tacggcgcca acgaaggctt ggtggcgcac
     2401 accgtattgg aagaggccaa agcgcatctg cgcgccaagc aagattttgt gtggaatgcc
     2461 actcacttgt cggtacaaat gcgtcaaaaa aacttggcgg cgtgtttggc ttacgatgcg
     2521 catgtgcgca ttgtgtgcgt ggaggccgat aaagccacgt tgctcaagcg taaccaagcg
     2581 cgtaacagca gtttgagcaa tgccaaattg ttgcaaatgc tcaagcattg ggaaatgccg
     2641 acactctttg aggcacacca attgaaagtg agtttgaatg ggttgtagtt aagtaaaagg
     2701 aattgttagg aggcattttc aggccgcgtg gtgtgcgcac cacgcaattg ggctggtttt
     2761 agccgtatta cctgttaaaa aatcccgttt tcagctgcga ttttgaatta agttcctaaa
     2821 atccatcatt tttttgtaaa attgccaaga tgaatgaact tctggctctg gatgcccctc
     2881 ctatcattaa tctgtaatct agatgttggt gaggtgctgt gatgttgagt gtggccgagt
     2941 tacgtcaacg caagcagcaa ttggaacaaa caattgccga attgatacag gtatttgagc
     3001 atgaaacttc ggttgaagta gacagtttgc tgactgttga gcgttgttca gtaaatacac
     3061 caatgcaaga tgccccaact tttcacatta aagtggatgt ccatttgaat atgtaagtgg
     3121 attttaatgg aaaaattggt tttggttgca taaccgtatc ccttccctga attgggttgg
     3181 cagttttggt ttgaatgttt atttttttat gagcattaaa attgaatttg agtataaatt
     3241 ttaagatgct tggattttta tccaagcatc tttcgcaaca tcttaaaaac aggtttaaaa
     3301 acatcgacaa tttcctataa attgtcattc tgccccatta tttgaagagc ccgcccatga
     3361 atgccgccat gaagattgcc agaatgtctc tggtacaacc gtttcgccca actgcgatgc
     3421 agaagccacg tctgacaccc atgccaaatt tgcacagcat gggcaaccga caagcgcatt
     3481 tggtgcaatt gtttgctgcg agaattgcgt tgcgtcaacg tgaactcagc agtggttttt
     3541 tggtgtttac cgacacaggt gaagtcaaat actatgcaga acaccattca tttgcggtgg
     3601 tgcatttggc cgcatttacc aacatgcctg tatacagcat caaatgacgc tatgtagata
     3661 acaaagcggc ctgtaaaaga cttttttgtt attgtcttgg taatgttttt ttccgcagcc
     3721 gtctcgtacg gctgtgagtt ttttgtggaa aacagcgaca caagtgtctg agtttgtctt
     3781 gtttgccgcc atcttgaaat ttttcaatcc tgcctcattt atctttcatc ttgattaacc
     3841 gttgttgcaa gcacacgagg gacacgatga aagaaacctt aagttttcaa accgaagtca
     3901 aacaattatt gcagttgatg attcattctt tgtattccaa caaagaaata tttttgcgcg
     3961 agttgatttc caacgccgcc gatgccacgg ataaattgcg ttttgaagcg ttgaacaatg
     4021 cggctttgtt ggaaaacgat ggcgatttac agattactgt tacctgcgat acggcagcaa
     4081 aaaccatcac catcactgac aatggcatag gcatgagccg tgatgaagtg attgaaaact
     4141 tgggcaccat tgccaaatcg ggcaccaaag cattttttga gcaattgtcg ggtgacgcaa
     4201 aaaaagacgc gaatctgatt ggtcaattcg gtgtgggctt ttactctgct ttcatcgtgg
     4261 ccgatgaagt ggaagtggaa acccgtcgtg caggcagcgc tgccaatgaa gcagtgcgtt
     4321 gggtgagcaa aggtgacggc gaattttcga ttgaaaacat cgacatgcct gagcgtggca
     4381 ccaaaatcac cttgcacatt aaagaagacg atgccgattt gctcagcgat tggactttgc
     4441 gccgcatcgt caccacttac agcgaccaca tttctgtacc gattttgatg aaaaaagcgc
     4501 ctgagtacga tgacgagggc aatattaccc attctgatga attggaagcg gtgaaccaag
     4561 cgcaggcttt gtggacacgc aataaaaacg acatcagcga tgagcaatac caagagtttt
     4621 acaaacacat ttcgcacgat tttagcgatg ctttggcatg gacgcatttc cgtgtagaag
     4681 gcaaacacga gtacacaggc ttgatgtatg tgccgaaaaa tgccccgttt gacatgtacg
     4741 accgcgagcc taaaggcgga gtgaaactgt atgtgaagcg tgtgttcatc atggacgatg
     4801 tgcaaaaact gatgcccatg tatttgcgct tcatcaaagg cattatcgat accaatgatt
     4861 tgcctttgaa cgtgtcgcgt gaaattttgc agcaaagccg tgatttggac caaattcgcg
     4921 caggtgcggt gaaaaaaatt ctgtccatgt tggaagacat cgccaaaaac cgcgccgaag
     4981 actacaccca attctggaac acatttggca gcgtgttgaa agagggcata ggcgaagacg
     5041 tggcgaacaa agacaagatt gcaggtttga gccgctttgc ttcaacacat aatgctgatg
     5101 ctgagcaaac cgtgtctttg gcagactata ttggccgcat gaaagaaggc caagacaaaa
     5161 tttactacat cacggctgaa acctatgctg cggcgaaaaa cagcccacac ttggaaattt
     5221 accgcaaaaa aggcatcgaa gtattgctgt tgaccgaccg cattgatgaa tgggtagtag
     5281 gcagcttgtt tgaatttgat ggcaagaaat tggccagtgt gtccaaaggt gagttggatt
     5341 tgggcagctt ggaaaacgaa gccgacaaag ccgagcaaga aaaagcccaa gaagcggcca
     5401 aggatgtggt tgccaaaatc aaagccgatt tgggcgataa agtggccgat gtgcgcgcca
     5461 ccacacgttt gaccgaaagc cctgcgtgtt tggtggtggg tgagcatgac atgagccaaa
     5521 acctacaacg catgttggcg gctgcgggtg cggcagacca attgcctggt gccaagcctg
     5581 tgttggaaat caatccagaa cacatgttgg tacagcgttt ggcgagtgaa tccaatggtg
     5641 tggtgcaagc agaattgtca caattgctgt acgaccaagc cttgcttgcc gaagggggta
     5701 aattggaaga cccagccagc tttgtgaaac gcatgaacca attgttggtg gcggcacacg
     5761 cataagccag ttaatggtat gaatcaatag caagcggcct gtaaatattt acaggccgct
     5821 tttttgtgtt ttcaaacaga tgtattcaac tgaatgattt gcagctcatt aagggcgggc
     5881 ttggcgcaaa tcttgaatca atgacaacaa atgctcctcg ctgacattgc tccaaacatt
     5941 gtataaacac ttttgtcctt tcactttcac ataagccaga tagtctggcc ctgtatcgcc
     6001 ttccaatccc caaccgatgc cgctgccatc gcgccaaaat cggtggttct cccagcgttg
     6061 tatgtcagcg ttgcttatgt tggcacctgt tcctgcaacg ccataagcac tgcgtttgcc
     6121 tgggatatcg taagccaccg cccagccgcc agaaaagctg gcagaccgac ttttggcttg
     6181 aggtaagtgg gcactggcat cacgaggcaa cactaacagc gcacaagtct tgcggttttc
     6241 agccttgcgc cattgctggg tgtaaacagg attgaattgc tgagtgcggg gatttttggc
     6301 ccaaggggcg tttttttctg aagtattggc cttggtgtcg gccaaggccg tgaaaggcac
     6361 tgtggcaagc agcaacaggc aaaaaggacg aaacatcatg tgcagcgctc ctttggtgga
     6421 atgtggcact atgttagcgt aaaaaataca cggtgtgttg cgtgtggggc ttgtgagcgt
     6481 agccgaaaat gggtaagttt gtatgccatg gagatgaaca ataggggaaa gacatggctg
     6541 tgttgtcagt gcaaccgata aatttgctgg aagttgattc gctgtaccaa caagtacagg
     6601 ccgagttgcc tgattcgatt cgactgcgag ttcaccgtgc catgagttgg ctcaaacaag
     6661 cgcagcagtc tgatacagag gacatgcggt ttatgtgttt gtgggtggct ttcaatgcgg
     6721 cctatgcacg cgacttacag gtgcgaacgg gtggtggtga tttggtgggc acgcgtgatt
     6781 ttttactgaa aatgtgtcgg tttcagccca aggtattgac ccatttggtg tgggatattt
     6841 accctcaagg catacgccaa ttgttggaaa accgttatgt gtttcaggct ttttgggatc
     6901 accacaatgg catgctcagt gaagaagcgt ggcaagccag ttttaacaaa gccaagcgca
     6961 aaggtttgga agccttaaag aataaggaca ccgccgatat cttgatgatg gtgtatgagc
     7021 gaatttacac cttgcgcaat caaatctttc acggtggtgc cactttcggt agcaaagtga
     7081 accgtctgca attgaaaagc gccttgggca ttttggccga caccataccc gtgtttttag
     7141 tggtgatgtt aaagcaccca aatgagactg tgtggggcag accctattat cctttggtca
     7201 aagattgagc taccaagcgg cctgtacagg ccggtggtct gagcctaacc atgggccgta
     7261 gctcactgtc gcgccatgcg gcgagcgcaa taagatgtgg tctatgctga tgtggcctgg
     7321 tcgccaagtg ggcatggcgc gcgaagtgtt tacggcgctg gggctggaca tnnnnnnnnn
     7381 ncaggctact gatttgggtg aagcggcgga atgagggtgc gaaagcggtg ttgttcatgt
     7441 cgcccatcac caatacctcg ccttgttcat ggctcacttg cttggctatg gtttgcaaca
     7501 cgcggtcgcg ctcggtggcc atgtaagccg aaattggtgg cggcgggtgt atggcgtaga
     7561 cgatgcggcc gttgcccaat tcggcacgaa tggcagggaa attttgcaag ctgccatgtt
     7621 gcaagacggt gcatgatttc aatgcgtgct tggacagcan nnnnnnnnna gccaaatggc
     7681 gagtcatcca agcgctcgca gccatgagga taagcagcct gtaaacgctg gacgatttgc
     7741 atggaaggcg cagcttcggt gagaaacacc acatcagcag actgcctctc cagccagttt
     7801 aaatcggttt caacctgtag gttgttgatg tagaggttgt aactgatgaa gcgctgttgt
     7861 gtgggcgcgg gcgttggtgg gttggccagt ggcgtgaaca tccatattgc caataacacc
     7921 gcgctcagtc caaaccagca tttttgccat gttttggggc tgaatagggt ggccagcaag
     7981 agtgttgcag cgtaataagg cacgaaatgg ctgaacaatt ccagtagcca ataaaaccga
     8041 cccaattccc ccaaaatcac agcgaccaga ctagcataag ccaaaaacac ggtgcgtttg
     8101 cgcacgaaag tgagtaagcg cgagctgttc atggtttaca ggacgctggt gacgctcaag
     8161 ccatcgtagg caacggcaat gccagctggg cagcgggctt gtaattcgtg gtagcctagg
     8221 cggtgggtca tgtggatgag gtatgtctgt ttggcggcaa tgcgctgagc aaaggcaaaa
     8281 gcccgttcta cgctcaagtg actggggtaa tcgtgctcca tcaggcaatc caaaaacaaa
     8341 taatccaaac cttgtaattg ggcgatgacc tcatcgctga tgtcgttcaa atcggtgagc
     8401 catgcgatgt tgcctatgcg aaacgccgac acgctccaac gaccgtgtgg cacggtaaac
     8461 gcctgcacag gcaggccagc gatggtttgc gcggcctgta aaccgtgggc tttgagcacg
     8521 ggtttgtccc aaaaatcacg tggttctaaa aatgcgtaat caaagcggct ttggatgttg
     8581 gccaaggtaa aaccattgcc atacacgggt atggcggctt tttgttggta gcaaaatgcg
     8641 cgcaaatcat cgatgccatt caaatggtcg gcatgtggat gggtgtataa caccgcatca
     8701 acatgcggga tgcgttcgcg caaggcttgg gtgcgaaaat cggtgcctgt gtcgatgacg
     8761 atggaggcac catcatcgct gtgtagccag gcagcgcagc gactgcgctg gttttcgggt
     8821 aggccactta aacacgtgtc gcaatcacaa ttgatgacag gcgtgccaga agagctgcca
     8881 caacccaata ccgttagggt gagcgtactc atgcgtttgc ctcaatgcgt ggcactttgt
     8941 tgaacaagcg gtagaagttt tcacaagtat agtgttccac ttcggcgaag ctttcaccgc
     9001 gcaattgtgc caaaaattca gcggtgtatt tcacgtaagc gggctcattg ggcttgccgc
     9061 gtttgggcac gggggccaag aatggcgcat cggtttccac caaaatgcgg tcttgcggca
     9121 caaatttggc ggcttcttgg atttgttgcg cgtttttaaa cgtcaagatg cctgaaaacg
     9181 agatgtagaa acccaaatcc aaggcttggc gagcaaagtc cacgtcttcg gtgaagcaat
     9241 gaatcacgcc tgcttgggct tgttcttcac tgagcaaacg caaagtatct gcgcctgcat
     9301 cgcgggtgtg cacaatcaaa ggcaaaccgc tggcattgct ggcttggatg tgggtacgaa
     9361 agcgattgtg ttgccaagtc aaatcgcctt ggcaccaatg gtaatctaaa cctgtttcgc
     9421 ctatgcccac cactttgggt aaagaagcgg cctgtaacaa ttcttctaag ctgaattcgg
     9481 cggcgttttc ttcatcaggg tgcacaccaa tggtgcagta gatgttttcg tgtgcattgg
     9541 ccaattgttg cacttgggca taactgtcgc ggctgacgct gatggccaaa gcttggcgga
     9601 tgtcgttgct gtgcatgttt gccaacactt cgggcaaacg cgccaccaaa tcgggaaaat
     9661 ttaagtggca atgtgaatcg atgagcatgg ttttaaatcg tgtaagtagg gcgaggtaag
     9721 tctaacaagc cagcaaggct gttttcaatg taatggcgtg cctgtttggc ggtatcactg
     9781 gcatcgaatg ccaagcccac accgcgtgga cgggtggaac tgccacgtat agggttgatc
     9841 cagcacactg tggttttgag cgggaaacgg tcggcggctt cgcccacttc caataccaac
     9901 aacacctcat cgcccatgtg gaattcgtca tcactggcaa tgaacacgcc accgttgttc
     9961 aaaaaactca tgtaagagtt gtaaatgctg gtgcggtctg ccaaagcaac ttgcaccatt
    10021 ttgccgggaa cttgtggttg tgccatgtta ttttgcctct ttatgcgtca tcaggttgag
    10081 atagtctatg cttaagtctt ctaattgtag cttaacattc aaagtgtgtt gcccaaacgg
    10141 catcagttga tgcattttat ccaccaattg ttgccacaat tgtggtgaat gttgggccaa
    10201 ggcgttcagg ccgctttgtt gctcgggata atacaatgct ggtaaacctt ggcgggccaa
    10261 gcccacatcg accagccatt tatacagcca atccaaagcc tctccgagcg gtcgcttgtg
    10321 cttatcgaat tcagtggcaa aatccaataa agccaatagg cgtggcttgg ccaaaattgc
    10381 caaccactgc gcgcgcaagt cttgcaaact gccttcttct tgcaacagtg gcgcacctgc
    10441 ttgaaacgcc aattggcttt tggaaagggt gatgccttgc tgttgtaaaa aattcaaagc
    10501 cacatcggtt gacggcgtgg gcaaatgcca cacttgcaag cggctcaaaa tggtgggtaa
    10561 caagcgttcg ctgtggtgcg ataccaagat aaacaccacg ttggcgggcg gttcttccaa
    10621 aacttttaac aaagcattgg cggcctgtag gttcaaggct tcggcaggct caataatgat
    10681 gacccgccgc ccgccttggt ggctgctcaa ttgggcaaat gccaatacct cgcgcacagc
    10741 atcgactttg atttgctgca gcttgcgggc attgccttct tcggtttcag gccgcatcac
    10801 catgcaatct ggatggctgt gttgcttgaa caagtgacaa gcagaacaca tgccacaagc
    10861 gtttttatcg gcgtcgatgt gtttgcacaa caaagcctgc gccaaatgtt ccgcaaatac
    10921 cattttaccc gtgccttgtt gaccactgag taaaatagcg tgtggtaagg cttgccagcg
    10981 ctgtgtggct tgttgccaca cggtttgttg ccaaggataa atcatgacta tgcaccctca
    11041 gcagcaaaca cacgggccac ggctgccaac acatcggcgc tgaccagctc gcgttcacgg
    11101 tgggcattga ttaaggcgta gcgctcaggc gcagcttgtg cccgttgctg gtaagcattg
    11161 cgcacgcggg tgaaaaattc ggcttgttct tgctcaaagc ggtctttctc acgcgatttt
    11221 tcaatgcgcc ccatggacac ttccaaaggc acatctaata acaaggtcaa atcaggctgt
    11281 aagccggcct gtacccatag ttctaaggtt gcaattttct gccaaggcaa gccgcgaccg
    11341 ccgccttggt aggcaaatgt ggcatcggta aagcggtcgc tgatgacatg ggtgccttgc
    11401 gctagagcag gctcaatcac atcttgcaag tgttgttggc gtgcggcaaa catcagcaag
    11461 cattcggttt cattgctaat gtgggtggct gggtcgagca gcaaggcgcg caaagattca
    11521 cccaaaggtg tgccaccagg ttcgcgggtc atcacaaatg gcaggttttg ctgcgtcatc
    11581 caagcttgta tggcttgtaa atgcgtggtt tttcctgcgc catcaatgcc atccaagctg
    11641 ataaacacgc cgcgttcagc gtttgcggat gtatttgttg acggcatcat tgtgttcctt
    11701 taaatcgtga ctgaaatagc tctcacctgt gctgtccatt ttggacacga aatacaggta
    11761 tttctcagca gagggatgtg ccgccgcttc caaagccgcg gtgctgggta aagagatggg
    11821 ggtagggggc aagccatcta ttacataggt attgtat
//
LOCUS       DAOMVK010000012        11697 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_1298_length_11697_cov_3.223501, whole genome shotgun sequence.
ACCESSION   DAOMVK010000012 DAOMVK010000000
VERSION     DAOMVK010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 11697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11697
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1298_length_11697_cov_3.223501"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..149
                     /locus_tag="PK856_00990"
     CDS             <1..149
                     /locus_tag="PK856_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRL00978.1"
                     /translation="IAGFGTFAISERAERTGRNPKTGEPITIAAAKKPAFKAGKALKE
                     AVNG"
     gene            217..630
                     /locus_tag="PK856_00995"
     CDS             217..630
                     /locus_tag="PK856_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein GemA"
                     /protein_id="HRL00979.1"
                     /translation="MENRRKKLIAKVKIAQQQLGLDDDTYRDLLEGAVGKRSAAKLNE
                     KELTTVIEVMKSKGFQDQASGHEADKPDVTEAKQALVGKIEALLADGNKPWRYAEAMA
                     ERMFNRKRIQWLNTDQLHRLVAALQINANREKEKS"
     gene            627..1094
                     /locus_tag="PK856_01000"
     CDS             627..1094
                     /locus_tag="PK856_01000"
                     /inference="COORDINATES: protein motif:HMM:NF020345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HRL00980.1"
                     /translation="MNELLEKDDFLGLAHLLPEGAKELVRLIGFEPTFLLIKHHGGTH
                     FPVGQNYNKNGKALFASLAEVIGEANAEKITLTFATKRHFFVPKCQVLMTELRRRKVR
                     AEFDELTKDQPAYMAVKDLARKYDLVESTIWYILKTTDVETITDISQMDLFSK"
     gene            1230..1763
                     /locus_tag="PK856_01005"
     CDS             1230..1763
                     /locus_tag="PK856_01005"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRL00981.1"
                     /translation="MGNIITITAGHSNTDPGAVNGIHREADKAQDMRNIVAYYLKQAG
                     VEFRVDGEGKGNLPLSKAIALIKGSKLAVEFHCNASSNKTAQGVEALSQLKDKSISQK
                     LCKAVADVMGIPLRGDRGWKPENSGQHSRLGYVANGGIILELFFISNDTELATWEERK
                     WLVGKAVATVLIEVANG"
     gene            1756..1950
                     /locus_tag="PK856_01010"
     CDS             1756..1950
                     /locus_tag="PK856_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00982.1"
                     /translation="MARKRNRYLLVGLLHVIALSLREASLPLQNLTYGRFAGYVTPER
                     VSRNSGVAQAKRAKRKAKQQ"
     gene            2196..2555
                     /locus_tag="PK856_01015"
     CDS             2196..2555
                     /locus_tag="PK856_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00983.1"
                     /translation="MKNKKPNGFGLLRLLNERFKAINTILLNKVDTSAKTVDFALSLH
                     ELVSRRDPEAQVMVNHNLDFVIVTGCPLPLLREDIANMGCYLIKAETDKKVWVMDLDE
                     CPNGGHRRITQLIQAEA"
     gene            2558..2971
                     /locus_tag="PK856_01020"
     CDS             2558..2971
                     /locus_tag="PK856_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00984.1"
                     /translation="MLTKIGQQLLEMLPGLFFCAMFFGSYLLGFSSGVNKMQEEVVQL
                     KLDHAEAKNTANELLVAQLTQSQSEMAKLQTKAQQTSKALAEANQTIQRQAAETKKGI
                     QDAIAKDKSAADGKCIDGLGANGLRQYNQAFGYTH"
     gene            2991..3161
                     /locus_tag="PK856_01025"
     CDS             2991..3161
                     /locus_tag="PK856_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00985.1"
                     /translation="MPPVPGELMVQPLRPNAPAKGTTQALLEHAVELGAYVGQLENQN
                     RAWRLWATGGTP"
     gene            3158..3403
                     /locus_tag="PK856_01030"
     CDS             3158..3403
                     /locus_tag="PK856_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00986.1"
                     /translation="MTIEMAFNILMSIAAAAIGLSVRRLFSQLDEQAKTIIDMRLDYQ
                     TRTEARQYMDDLKQGLADIKADLNKLNDKLDKKADKP"
     gene            3400..3657
                     /locus_tag="PK856_01035"
     CDS             3400..3657
                     /locus_tag="PK856_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00987.1"
                     /translation="MNENNNETIALLRKIDSKVDALGEQVSKVQKIAEEAGAKAGANA
                     GKKSGALYGLIAGTAGGAVGGTVGAGLLETGIAMLKAKWGM"
     gene            3658..4155
                     /locus_tag="PK856_01040"
     CDS             3658..4155
                     /locus_tag="PK856_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003797422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HRL00988.1"
                     /translation="MAHPQAVRDKLRQAYVFDQHPLEIAALQAGVGFGTARRWKADAK
                     ESGDDWDKVRAAHVLAGNSMEDLSRGILSAFLLQFNATMTDLQTSDLPPGEKVDKLTS
                     LADAFNKTAAASKKVLPETNQLATAMQVLQKLGEFIQVKHPQHLALFAEILEPFGAEL
                     QKMFS"
     gene            complement(4152..4715)
                     /locus_tag="PK856_01045"
     CDS             complement(4152..4715)
                     /locus_tag="PK856_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00989.1"
                     /translation="MINWLDRNLQLDILKYLQPLYPNEVSSKNIAAKLFPEHSFTFEP
                     RVNEGMADAMGIELKPEDDKYTQIQKNIHYLYDSGLINLKIQKTMSFIKYECSINNRG
                     IDFLEDDGGLSSILGVVTIKLHADTIKALIQQKIEAADITDSEKSALKSTVSKIGDAA
                     LATLTEKAIEAVPIMYFFSVLKSLGNG"
     gene            4735..6342
                     /gene="terL"
                     /locus_tag="PK856_01050"
     CDS             4735..6342
                     /gene="terL"
                     /locus_tag="PK856_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HRL00990.1"
                     /translation="MKTKEFLQSLVDYAAQLRRMVEAEVDGFDPSPEAQTERRNRVLD
                     PVNGFEYFVNTYFPHYVRRPEKSQFHEYLFERLPEVLRAEKGKHEVVAAPRGEAKSTL
                     VTQMFDLWCIVTGLKKFIVIVMDSIDQAYPMLEVIKTELEFNSRLIGDFPESTGQGRV
                     WQAGTIVTANNIKVQVAGSGKKLRGLRHGPYRPDLVVLDDIENDEQVRNPVQRDKLES
                     WLKMTVLPLGGVGQKFDVIYIGTILHYDSVLSRTLKNPGWNGVKFKALVSYPNNMVLW
                     DKWEELYNNEGEKVATAYYETHKKAMDAGAVTSWAARGVLDLMTIRARDGHATFDSEY
                     QNDPVSGDNAPFANSIQFWVNRLDEWIFYGSLDPSLGKAGNSRDPSALLVGGLNKRTG
                     ILDVVEALIKKRLPDRIISDTIELQKQYNCLLWAVETVQFQEFLKDELVKRSAAMGIP
                     VPARGVKPTADKHLRIESLQPHMQNGLLRLHPSQSTLIEQLRHFPMAAHDDGPDALHM
                     LWMISKTGIATSNARVIHIPEPNLPMF"
     gene            6376..7794
                     /locus_tag="PK856_01055"
     CDS             6376..7794
                     /locus_tag="PK856_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00991.1"
                     /translation="MKFRKKKIAATVQTLITDTDNALESLMGSGESADQLLTRLGVSR
                     QQAFETISSDDEVESCREDLRTAMLSKTFRLWGEDANDDEINRLWRIMRKHLPVLVEA
                     VLTAKLNGYAVIRYVYEQEEDGFLSVGKVSDKSTELDKYTPKADGSLTYYSDNGEVTV
                     DTNVVFLLLTNRATSTNPAGEMAGARLYPAVAIRKQGFIYAAQFIKRYAQPYMVGYVE
                     GETETFSSKLFSFIAGGAIAVNREDRIEMLQNNADGQAFQRLEQVANRRIQKALLGKV
                     KTSDLENGSRAAQETEEETKGDRIDGYLYMLGLAAQHMVDALLMVNEAYGKTIHAPKG
                     LWFEFNKKTEIDIKRADRDTKYAKDANLRFTEDYYIDVLGFEPNHFVLAEEATAPTSG
                     NASLSARLSDKYKQGNPLNAFSQPKIDAIASALDECESFADFEAKLSGMDLSEGDNAL
                     IQRVLGDAVHEFIKGSEAQNHG"
     gene            7787..9088
                     /locus_tag="PK856_01060"
     CDS             7787..9088
                     /locus_tag="PK856_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HRL00992.1"
                     /translation="MDEIELNPIGLIDRAALEFLKSKKLLPSFDYQDVWMHEHAVAFT
                     VAKMLDQDLLAEVKTALEDAITSGSDFNAFKKRLKPYLMSRGWWGEQIMVDPLDEQAK
                     VVQLGSTRRLETIFQTNMATAYAAGQWQRIQSTKSALPYLRYNASAASNPREAHKRYY
                     GLVLPVEHELWNTIYPPNGYGCLCTVIQLTRKQAEKYGISDEPDVEVVEVVNQRTGEV
                     VNIPADITPSFAHNHGDRLGALNALFGDKHGTDALDSLIQSRESWLAKRFSMPDSIKT
                     TILPAKVSKKELNRLLVDVLDNIAKSSEADAAARWQVAHNVKLERYTLDNETPPDFYI
                     VDADKPKGEWTTIDFMYTMYTQDPSNLFKVEKFNQYFADTELRWKDHQLNIQKHLAKA
                     DIVPLDLRQLNQLNRMKLVAYVLSLPQEQQAKIQFLVSESL"
     gene            9085..9390
                     /locus_tag="PK856_01065"
     CDS             9085..9390
                     /locus_tag="PK856_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL00993.1"
                     /translation="MSIDNLIIHADGAIRDVQLSKRAVYDLFNFMLPKLQTQLPGFSD
                     EFEGQYYGEVLSISHVPAEHFPLVSDFIMQACDEFDSLKPHKAVLQEALQSDPRHKQ"
     gene            9646..10623
                     /locus_tag="PK856_01070"
     CDS             9646..10623
                     /locus_tag="PK856_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter C-terminal domain-containing
                     protein"
                     /protein_id="HRL00994.1"
                     /translation="MTTKTPTPQKNIEAKLSSGINVDINPVNADGPRKFSGVATSGLP
                     FSLWGWNTVIDLDNIQHKPKVAILHEHKQPAGSGELSVDPDKGLLVTGTFLSNEWGQF
                     IAQASDEGFPWEMSVHVVAGRSEELAAGATTTVNGKEVSGPLSIWRDCVVREASFTAV
                     GVDQNTSATALSGLLPQPSEEHVPMTPEEQKEMDALKTKVATLEQEKADLETANQKLE
                     TEKADAEKAAQEAEVDAELSAAGFQRHEDGKGFQGVSAATYGVLLSAKPDDAKAMIAD
                     LAPGGHGHKPAVPGGLLSDSTLKDKPHGAALSGLAANAAERKNNGANYV"
     gene            10616..10960
                     /locus_tag="PK856_01075"
     CDS             10616..10960
                     /locus_tag="PK856_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003741297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase alpha chain"
                     /protein_id="HRL00995.1"
                     /translation="MSKSNPATLGPSISDFLKWEATRLTRTTVPAAIGTKAGALVTFD
                     LRDMPLVALTDESDGQVVVQPHSCVIDLSLISEAAINTALGGTEELPKTVEDLAKQGD
                     AFGIVYQGTPLA"
     gene            10980..>11697
                     /locus_tag="PK856_01080"
     CDS             10980..>11697
                     /locus_tag="PK856_01080"
                     /inference="COORDINATES: protein motif:HMM:NF015802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HRL00996.1"
                     /translation="MPLSSDSRFGVQAMTDAINALPVNPTQIRSLGLFTPKPLTTTHV
                     DVEHLEGTLKLVQSKERGLPGEPVASRKRNIRTFKIPHLPVDDVIRADEVQNVRAFGG
                     TNAQTVDSVVNDKLADSKSNLEFTREHMQLGALQGKILDANGDLLYDIYDEFKIQRKV
                     FQWKLGTAGTKVGAVMDETSTDMRKHLKGEFLTKWLVLCSPEFMAQLKYHDSIKELYE
                     RYRDGAAYREANENQIMFEHN"
BASE COUNT         3422 a         2606 c         2812 g         2857 t
ORIGIN      
        1 cgattgcagg ctttggaaca tttgcaatca gtgaacgtgc agaacgcaca ggccgcaacc
       61 ccaaaacagg tgaacccatc accattgccg ccgccaaaaa accagcattc aaagctggta
      121 aggctttgaa agaggctgtt aacggttaaa acccaaatcc taagcggttg gcaacagccg
      181 tttagtgtgt gcgttttatc aaaacaggag agattaatgg aaaaccgtcg caaaaaactg
      241 attgctaaag tcaaaatcgc ccaacaacaa cttggtttgg atgacgatac ctaccgcgat
      301 ttactggaag gcgctgttgg taagcgctca gccgccaaat tgaacgaaaa agaattgacc
      361 acggtgattg aagtcatgaa aagcaaaggc ttccaagacc aagccagtgg ccacgaggca
      421 gacaaacccg atgtgacaga ggctaaacaa gcactggtgg gcaaaataga agccttattg
      481 gccgatggta acaagccatg gcgttatgcc gaagccatgg ccgaacgaat gtttaaccgc
      541 aaacgcattc agtggttaaa caccgatcaa ctgcatcgct tggtagctgc cttacaaatc
      601 aatgccaacc gagaaaagga gaaatcatga acgagttgtt ggaaaaggat gactttctgg
      661 gcttggcaca cttgttacca gaaggcgcga aggagctggt gcggttgatt ggattcgagc
      721 ccactttttt gttgattaaa caccacggtg gcactcactt tccagttggt caaaactaca
      781 ataaaaatgg caaagcctta tttgcctcac tggctgaagt cataggcgaa gccaatgctg
      841 aaaaaattac cctgaccttt gccacaaaac gccatttctt tgtgccaaaa tgccaagtgc
      901 tgatgactga attgcgccgc cgcaaagtaa gagccgaatt tgatgagtta accaaagacc
      961 agcccgctta tatggcagtg aaagatttgg ccagaaaata tgatttggtc gaaagcacca
     1021 tatggtacat tttgaaaacc actgatgttg aaaccatcac agacatcagc caaatggatt
     1081 tattttccaa ataattgaag cccccaagtt taaacagctt gggggttttt tcttgtactc
     1141 tcattcagta agacacaccc aaaccatcac ctaaccataa tgaaacccta tacagattaa
     1201 tttgatattc aacttaataa agggttttta tgggcaacat catcaccatc accgctggcc
     1261 acagcaatac cgatcctggt gccgtgaatg gcattcatcg cgaggcagac aaggcgcaag
     1321 acatgcgcaa tatcgtggcc tattacctga agcaagctgg cgtggaattt cgtgtcgatg
     1381 gcgaaggcaa aggcaatttg ccattgagca aagccattgc cctaatcaaa ggcagcaaac
     1441 tggcagttga gttccattgc aatgcctctt caaacaaaac cgcccaaggc gttgaagcct
     1501 tatcccaact caaagacaaa tccatcagcc aaaagctatg caaagccgtt gccgacgtca
     1561 tgggcattcc attgcgcggt gatcgtggct ggaagccaga aaactcgggg cagcatagtc
     1621 gtcttggtta tgtggccaac gggggcatta ttttagagct gtttttcatc agcaatgata
     1681 ccgaactggc tacttgggaa gaacgcaaat ggttggttgg caaagcagtg gccaccgttt
     1741 tgatagaggt tgccaatggc taggaaacgc aaccgttacc tgttggtggg tttgcttcat
     1801 gtgattgcgt taagtctgcg tgaagcaagc ttgccattac aaaacctgac ttacggccgt
     1861 tttgctggct atgtcacccc tgagcgcgta agccgcaaca gtggcgtggc acaagccaaa
     1921 cgtgcaaaaa gaaaggcaaa gcagcagtga acaatccact tacatcacct tgggtgaagt
     1981 catttattaa agctttggcc ggtaatgaag tcactggagc cccatcgcat accaaaattt
     2041 gggccagtgt tgcctgcgcc actgccactt ggaagtttat tcagatccct gagccttcgg
     2101 tagttatttg ggtttggtcg gcgggtatgc attggggcgt cgttggatag ctgccaagca
     2161 agatgtggaa accaaaaaaa ttgaaggtgc caacaatgaa gaataagaaa cccaacggct
     2221 ttggcttatt gcgtttactg aatgagcgct tcaaagccat taataccatt cttttaaaca
     2281 aagttgatac aagcgccaag acagtagatt ttgcattgag cttacatgag ttggtgagta
     2341 gacgagaccc agaagcacag gtgatggtca atcataactt ggattttgtg attgtcactg
     2401 gctgtccatt gccattgtta cgagaagaca ttgcaaacat ggggtgttac ttaatcaaag
     2461 ccgaaaccga taaaaaagta tgggtgatgg atttggatga atgccccaat ggtggccatc
     2521 gccgcatcac ccaactgata caagcggagg cataaagatg ttgaccaaaa ttgggcaaca
     2581 gcttttagaa atgcttccag gcttgttttt ttgtgcgatg ttctttggtt cctatttgct
     2641 tggtttttcc agtggcgtga acaaaatgca agaagaagtg gtgcagttga aactggacca
     2701 tgcggaagcg aagaacaccg ccaatgagct gcttgtggca cagctcactc aatcacaaag
     2761 tgaaatggcc aagctgcaaa ccaaagccca gcaaaccagt aaagcattgg ccgaagccaa
     2821 tcaaaccata cagcgccaag ccgctgaaac caaaaaaggg attcaagatg cgattgcaaa
     2881 agataaaagt gctgctgatg gtaagtgtat cgacggcctc ggcgctaacg gcttgcggca
     2941 atacaaccaa gcctttggat acacccatta aggtgattga aaaagcagtg atgccaccag
     3001 tgccaggtga attgatggtg caaccattgc gccccaatgc ccccgccaaa ggcaccaccc
     3061 aagccttatt agaacatgcg gtcgaacttg gtgcctatgt gggccaatta gaaaaccaaa
     3121 accgagcatg gcgcttgtgg gccactggag gcacaccatg accatagaaa tggcttttaa
     3181 cattttgatg tcaattgccg ctgctgcgat tggcttaagt gttcgacgtt tgttttcaca
     3241 gcttgatgaa caagcaaaaa cgattattga catgcgactt gattaccaga cccgtactga
     3301 agctcgtcag tatatggatg acttaaaaca gggactggct gacatcaaag cagacttaaa
     3361 caaactaaac gataaattgg acaaaaaagc agacaagcca tgaacgagaa taataacgaa
     3421 acaattgctt tacttcgcaa aattgacagc aaagttgatg cattaggaga gcaagtaagc
     3481 aaggtgcaaa aaattgctga agaagcaggt gcaaaagctg gggcaaatgc tgggaaaaag
     3541 tcaggtgcat tgtatgggct gattgccggt acagctggcg gtgccgttgg cggaactgta
     3601 ggcgctgggc ttcttgaaac aggcattgct atgcttaaag ctaaatgggg aatgtagatg
     3661 gcacaccctc aagctgtgcg cgacaagcta cgacaggcat atgtgtttga ccaacacccc
     3721 ttagaaatag ctgcattaca agctggcgtt ggttttggaa ccgcaaggcg ctggaaagct
     3781 gatgccaaag aaagtggtga cgattgggac aaggtacgtg ctgcccatgt gcttgctgga
     3841 aatagcatgg aagatttaag ccgcgggatt ctcagtgcct ttttgctgca atttaacgcg
     3901 accatgactg acttgcagac cagcgactta ccgccaggag aaaaagtaga caaattaacc
     3961 agcttggcgg acgcatttaa caaaactgcg gcggcaagta agaaggtctt gcctgagacc
     4021 aatcaacttg ccaccgcaat gcaggttttg caaaagctag gggagtttat ccaagtaaag
     4081 cacccgcaac atttggcctt gtttgctgaa attttggagc cgtttggtgc tgaactgcaa
     4141 aagatgttca gttagccatt acctagtgat ttgagcacgg agaaaaaata catgattgga
     4201 actgcttcta tagccttctc tgtaagggta gctagtgcag cgtctccgat tttagatacg
     4261 gttgatttta aggcggattt ttcagagtcg gtaatatcag ccgcctcaat tttttgttga
     4321 atgagcgctt taattgtatc tgcatgtaac ttaattgtaa ccactcctag tatcgaagaa
     4381 aggccaccat catcttctaa aaagtcgata ccacgattat ttatagaaca ttcatattta
     4441 ataaaggaca ttgttttttg aattttaaga ttgattaaac ctgagtcata caagtagtga
     4501 atgttttttt gaatttgtgt gtatttatca tcctctggtt ttaactctat acccatagca
     4561 tcagccatac cttcattaac acgaggctca aatgtaaaag agtgctccgg aaatagtttt
     4621 gcagcaatgt tttttgaaga tacttcatta gggtacagag gctgtaaata tttcagaata
     4681 tccagctgca gattacggtc tagccaatta atcattaatg aaagtccttt taaagtgaaa
     4741 accaaagaat ttttacagtc tttagttgat tacgcggccc aactcagacg tatggtcgaa
     4801 gctgaggttg acggttttga cccatcacca gaagcacaaa cagaacgccg caacagggtt
     4861 ttggaccccg ttaacggttt tgaatatttt gttaatactt atttcccgca ttatgtacgt
     4921 cgcccagaaa aatcacaatt tcacgagtat ttgtttgagc gtttgcctga agtgttgcgc
     4981 gctgaaaaag gcaagcacga agtagttgca gctcctcgtg gtgaagccaa atcaaccttg
     5041 gtgacacaga tgtttgacct gtggtgcatt gttacaggcc tgaagaaatt tattgtcatt
     5101 gtgatggaca gcatagacca agcttaccca atgcttgaag tcattaaaac cgagcttgaa
     5161 tttaattccc gtttgattgg tgattttcct gaatccacag gccaaggccg agtgtggcaa
     5221 gccggcacaa ttgtgaccgc aaacaatatc aaggtgcaag ttgccggctc aggcaaaaag
     5281 ttgcgcggtt tacgccacgg cccataccgt cctgatttgg tggttcttga tgacattgag
     5341 aatgatgaac aagtaagaaa ccctgtgcaa cgggataaat tagagagctg gcttaagatg
     5401 accgtattgc ctttgggcgg tgttggccaa aagtttgacg tgatttacat aggcacaatc
     5461 ttgcactatg actccgtctt aagccgaact ttaaaaaatc ctggctggaa tggtgtgaaa
     5521 tttaaagcct tggtttccta cccaaataac atggtgttat gggataagtg ggaagaactg
     5581 tacaacaatg aaggtgaaaa agtcgctacc gcttattatg aaacccataa aaaggcgatg
     5641 gatgcgggcg cggtgacaag ctgggccgca cgcggtgtat tagacctgat gaccatccgc
     5701 gcccgtgatg gccacgctac atttgattcc gaatatcaaa atgaccctgt atcaggtgat
     5761 aacgccccat ttgctaacag catacagttc tgggtgaacc gcttagatga gtggattttt
     5821 tatgggtcgc ttgatccatc gcttggtaaa gctggcaact cacgcgaccc gtcagccctt
     5881 ttggttggcg gcctgaataa acgcactggc attttagatg tggtcgaagc tttgattaag
     5941 aaacgcctac ccgaccgcat catatccgac acgatagaac tgcaaaaaca gtacaactgc
     6001 ttattgtggg ctgttgaaac cgtacagttc caagagttct taaaagatga actggtaaaa
     6061 cggtcagctg cgatgggcat acctgtgcca gctcgtggcg ttaagccaac tgctgataag
     6121 cacttacgca ttgaatccct acagccacac atgcaaaacg gtttactgcg actgcaccca
     6181 agccaatcca cgttaataga gcagctccgg cattttccaa tggctgccca cgatgatggc
     6241 cccgatgcgc tccatatgct ttggatgatt tctaaaacag gaattgcaac cagcaatgct
     6301 cgtgttatcc acatcccaga acccaatttg cccatgtttt aaacggcctg aaagtattga
     6361 ggtgacttta tgttaatgaa atttagaaaa aagaaaattg cggcgacggt tcaaacactg
     6421 attactgata ctgacaatgc attagaaagc ttgatgggca gcggcgaaag cgcagaccaa
     6481 ttgttgacac gcttgggtgt tagccgccaa caagcatttg aaaccatcag ctctgacgat
     6541 gaagttgagt catgccgtga agacttacgc acggcaatgc tgtcaaagac ttttagactg
     6601 tggggagaag atgccaacga cgatgaaata aaccgcttat ggcgcatcat gcgcaagcat
     6661 ttgcccgtat tggttgaggc cgttttaacc gctaaattga acggttatgc agtcatacgc
     6721 tatgtatacg aacaggaaga ggacggattc ttaagtgttg gtaaagtcag cgacaaatca
     6781 actgaattgg ataagtacac gcccaaggct gatggcagct taacgtatta cagcgataac
     6841 ggcgaagtca cagtagacac caatgttgtg tttctgttgt taaccaaccg cgcaacaagc
     6901 accaatccag caggtgaaat ggccggggca cgcctttacc ccgctgtggc catacgcaaa
     6961 caaggcttta tctacgccgc gcaattcatt aaacgatatg cacagcccta tatggttggc
     7021 tacgttgaag gcgaaacaga gacctttagc agcaaactgt tttcgttcat cgccggcggc
     7081 gcaatagcag tcaaccgcga agatcgcata gagatgttgc aaaacaacgc tgatgggcaa
     7141 gcctttcaac gcttggaaca agtggccaat cgccgcatac aaaaagccct attgggtaaa
     7201 gtcaaaacat cagatttgga aaacggcagc cgtgcggcac aggaaacgga agaagaaacc
     7261 aaaggcgacc gaatcgatgg ctatttgtac atgcttggtt tggccgcaca acacatggtc
     7321 gatgctttgc tgatggtgaa tgaagcctat ggtaaaacca tacatgcacc caaaggcttg
     7381 tggtttgaat ttaacaaaaa gactgaaatt gacatcaagc gtgccgaccg tgacaccaaa
     7441 tatgccaaag atgccaattt gcggtttacc gaagattact acattgatgt gttgggattt
     7501 gaacctaatc actttgtgct ggctgaagaa gctaccgcac ccacttctgg caatgcatcg
     7561 ttgtcggcca ggctgtcaga taaatacaag caaggcaatc cactgaatgc gttttcacaa
     7621 cccaagattg atgccattgc ttctgcccta gacgagtgtg agtcgtttgc tgactttgag
     7681 gccaagctgt cagggatgga tttaagcgaa ggcgacaatg cgttgatcca gcgtgtgctt
     7741 ggtgatgcgg tgcatgagtt tatcaaaggc agtgaggctc aaaaccatgg atgaaattga
     7801 actaaacccc attggactga tagaccgtgc tgccttagag tttttgaagt ctaaaaagct
     7861 gttgcccagc tttgattacc aagatgtgtg gatgcatgag catgcggtgg cttttacagt
     7921 ggccaaaatg ctggaccaag acttgttggc tgaagtgaaa acagctttgg aagacgccat
     7981 tacttctggc agtgatttca atgcgtttaa aaagcgttta aaaccgtatt taatgagccg
     8041 tggttggtgg ggcgagcaaa ttatggtgga tcctttggac gagcaagcaa aagtcgttca
     8101 gcttggcagt actcgccgtc ttgaaaccat tttccaaacc aacatggcca cagcatacgc
     8161 agctggacaa tggcaacgaa ttcaatccac caaatcagct ttgccatatt tgcgttacaa
     8221 cgccagtgcg gccagtaatc cacgagaggc ccacaagcgc tattacggtt tggtgttgcc
     8281 tgttgagcat gagctgtgga ataccattta cccaccaaac ggctatggct gtttgtgtac
     8341 tgtgatccaa ctgacacgca agcaggccga aaaatacggc atttcagatg agccagatgt
     8401 tgaagtggtc gaagtggtga atcagcgcac tggtgaagtg gtcaatatac ctgctgatat
     8461 tacccccagc tttgcccaca accatggtga tcgccttggt gcattgaatg ctttgtttgg
     8521 tgacaagcat ggcacagacg ctttagacag cttgattcaa tcacgagaat cttggttagc
     8581 caaacgattc agtatgcctg acagcatcaa aaccaccata ttgcctgcaa aggtttccaa
     8641 gaaagagctt aaccgtctat tggttgacgt gttggacaat atcgccaaat cctccgaggc
     8701 cgacgcagca gctcggtggc aagtcgctca caatgtgaaa ctggagcgtt atacactgga
     8761 caatgagacg cccccagact tttatattgt tgatgctgac aaacccaaag gggaatggac
     8821 cacgattgac ttcatgtaca ctatgtacac tcaggaccca agcaatctgt ttaaagttga
     8881 aaaatttaac caatactttg ctgatactga gctgcggtgg aaagatcacc agctaaacat
     8941 ccaaaagcat ttggccaaag cagatattgt tccattggat ttacgtcaat tgaatcaatt
     9001 aaaccgaatg aaattagttg cctatgtgtt atcattgccg caagagcaac aagcaaaaat
     9061 acaattttta gtaagcgagt cattatgagt atagacaatt taatcatcca tgcagacgga
     9121 gctatccgtg atgtgcagtt gagtaagcgt gctgtctatg atttgtttaa cttcatgttg
     9181 cccaagctgc aaacccagtt gcctggtttt agcgatgagt ttgaaggtca atactacggt
     9241 gaggttttgt caatcagcca tgtgcctgcc gaacacttcc cacttgtttc agatttcatc
     9301 atgcaagcat gtgatgagtt tgacagtttg aaaccccata aagccgtctt acaagaagcc
     9361 ttgcaatctg acccacggca taagcaataa gtcttaaaat tagaaaccgc cacagaagcc
     9421 cttaaacggg cttttttcat gcgagtaatg caaaggtata cccaaggcat taaacccctc
     9481 ttaaagacgc tctttacagg gtctttacag ccatcgcaaa tacaagacag agttacaaag
     9541 tcctatttgg taagtttcat ggctttggcc tgcttatttc cccctgattc tcgtactcgt
     9601 atcccgtcag acacctgaat accatcatgg cggcacaatt gccccatgac tacgaaaacc
     9661 cccacccccc aaaaaaacat tgaagccaag ttgtccagtg gcatcaatgt agacatcaat
     9721 ccagtcaacg cagatggtcc acgtaaattc agtggtgtgg ccacaagcgg ccttccattt
     9781 tcactttggg gctggaatac cgttattgac ctcgacaaca tccaacacaa gcccaaagtg
     9841 gccatcttgc atgaacacaa gcaaccggct ggcagtggtg agttatcagt agaccccgac
     9901 aaaggtttat tagtaactgg cacttttttg agcaatgagt ggggtcagtt cattgcccaa
     9961 gcttcggacg aaggcttccc ttgggagatg tcagttcatg ttgttgcagg ccgttctgaa
    10021 gagttggccg caggggctac cactaccgta aatggtaaag aagtgtctgg accattgagc
    10081 atttggcgcg attgtgttgt gcgtgaagca tcatttacgg ccgtgggggt ggatcaaaat
    10141 acaagcgcta cggcgttatc tggtttatta ccacaaccat cagaggagca tgtacccatg
    10201 acacccgaag aacagaagga aatggacgct ttgaaaacca aagtggcaac actggagcaa
    10261 gaaaaagcgg atttagaaac agccaatcaa aaattggaaa ctgaaaaagc agacgctgaa
    10321 aaagcagcac aagaagccga agtggatgcg gagttgtctg cggctggttt ccaacgccat
    10381 gaggatggca aaggctttca aggtgtttca gctgccactt atggtgtgtt gttgtcggcc
    10441 aagccggatg acgccaaagc gatgattgcc gacttggcac ctggcggcca cggccacaaa
    10501 cctgcagtgc ctggtggctt gttaagtgac tcaactttaa aagacaagcc gcatggagct
    10561 gctttgtcgg gattggctgc caatgcagca gagcgcaaaa acaatggagc caactatgtc
    10621 taaaagtaac cctgccaccc ttggtcccag catcagtgat tttttgaagt gggaagccac
    10681 ccgtttaacc cgcaccacag tgcccgcggc cattggtaca aaagccggtg ctttggtgac
    10741 gtttgatttg cgtgacatgc cgcttgtggc attgacggat gaaagcgatg gccaagtggt
    10801 ggtgcaacct cacagttgcg tcattgattt atcactgatc agtgaagccg ccatcaatac
    10861 agctttgggt ggcactgaag aattgcccaa aacggttgaa gacttggcca aacaaggtga
    10921 tgcctttggg attgtctacc aaggcacccc attagcctaa taacaaaagg aaaacaaaaa
    10981 tgccattaag cagcgacagc cgtttcggcg ttcaagcaat gaccgatgcg attaacgcat
    11041 tacccgtgaa tccaacgcaa atccgcagct tgggtttgtt cacacctaag ccattaacca
    11101 ccacccatgt ggatgttgag cacttggaag gcacattgaa gttggtgcaa tccaaagaac
    11161 gtggtttgcc tggcgagcca gttgcaagtc gcaagcgcaa tattcgcact ttcaaaatcc
    11221 cacatttgcc ggttgatgat gtcattcgtg ccgatgaagt ccaaaacgtg cgtgcgtttg
    11281 gtggcaccaa tgcccaaacc gttgactctg tggtcaatga caaattggcc gactccaaat
    11341 ccaatttgga attcacccgt gagcacatgc aattgggtgc actccaaggc aagattttgg
    11401 atgccaatgg cgatcttttg tatgacatct atgatgaatt caaaattcaa cgcaaagtgt
    11461 tccaatggaa attgggtacg gcaggcacaa aagtcggtgc ggtgatggat gaaacatcaa
    11521 ccgacatgcg caaacacttg aaaggtgagt ttcttaccaa gtggttggtg ttgtgtagcc
    11581 cagaattcat ggcgcaattg aaataccacg actccatcaa agagctgtat gagcgctatc
    11641 gtgatggtgc ggcataccgt gaagccaatg aaaatcaaat catgtttgaa cacaatg
//
LOCUS       DAOMVK010000013        11655 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_1302_length_11655_cov_3.281897, whole genome shotgun sequence.
ACCESSION   DAOMVK010000013 DAOMVK010000000
VERSION     DAOMVK010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 11655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11655
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1302_length_11655_cov_3.281897"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..1321)
                     /locus_tag="PK856_01085"
     CDS             complement(62..1321)
                     /locus_tag="PK856_01085"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HRL00997.1"
                     /translation="MNNPQLIHTERLIAEVSLFRSPADMVLSKFFKDHKKLGRQDRHE
                     IAETVYAALRHWQKLHMVFGAKAPARLLALGALVIGRGVSIASLSDIINEAERTALSG
                     LKAAKSEFTGRLNTASELPEWLVERMQGQYSDEEIIAIGQSLSQAAPLDTRVNTIKGK
                     REKVLAQLEADGFKAAATPFSPWGIRFQDKAAINKNALFLDGFLEVQDEGSQLLALIS
                     GAKRGEVVVDFCAGAGGKTLALAAMMNNTGRLYAMDVSEKRLANIKPRIARAGVGNIT
                     PQRLDHERDLRLKKLWNKADRVLVDAPCSGLGTLRRNPDLKYRQSNDSIDELVTLQTR
                     ILDSAAHLVKRGGFLIYATCSILAEENQKQVEHFLSSHPDFQLVNCSDLLKSNKIALD
                     TGEFLQLNPATHHTDGFFAAVMQKSSS"
     gene            complement(1322..1999)
                     /locus_tag="PK856_01090"
     CDS             complement(1322..1999)
                     /locus_tag="PK856_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003824640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HRL00998.1"
                     /translation="MQHFIKALGMTTVLLASSAYAETVSFPKNAALTYSGNGGVTATM
                     AYGRSASSYNINTTFKIPLYSMQFRSSGSMSGNTMVPNSYSDTRRGKLYAQAKFNHGS
                     KTITYGKAGENKTAPMRGVPMDLFSLAWQLALNNGKVSGVSQFTNGKKVYNEATNIRA
                     AGTQNVKFKGKPLSVNMYRATRGGDVMEYGLAPSLGNIPALIRYTDDGKTYQLNLTAA
                     TLDGKKY"
     assembly_gap    2068..2167
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2196..2828)
                     /gene="purN"
                     /locus_tag="PK856_01095"
     CDS             complement(2196..2828)
                     /gene="purN"
                     /locus_tag="PK856_01095"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HRL00999.1"
                     /translation="MNKNVVILISGRGSNMQAIVNAGIAGADIKAVISNRPDAAGLAW
                     ASERGITTEVVNHRDYTSREAFDAVLQQTIDAYQPDLVVLAGFMRILTADFCLHYQNR
                     MLNIHPSLLPSFKGLHTHQAAIDAGCKLAGCSVHFVTPELDHGPIISQGAVPILPDDT
                     ADSLATRTLIVEHSLYPQAVADFVNNRLHIKDGKVINQQPAPAESKLLAF"
     gene            2913..3728
                     /locus_tag="PK856_01100"
     CDS             2913..3728
                     /locus_tag="PK856_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HRL01000.1"
                     /translation="MAFDIKRFLVDGDKKWQWKDYPCKYSKDIPKAKEDRNKLAAELS
                     TELDGLQDRLYGEGKRKLLVILQGMDTSGKDGTIRHVFQNVDPLGVYVEAFKAPSEAE
                     LARDYLWRVHQVVPKNGEIAIFNRSHYEDVLVTRVKEWIDADTEAKRIDHINAFETLL
                     SDTGTEVVKIFLNISKDEQRERLQQRIDDPSKNWKFNLGDLEDRQLWDKFQSQYARVM
                     RKTSSKNAPWYVVPADSKSTRNIIVLQILLHHLRQMNPQYPMVDTSAWPKQIK"
     gene            3746..4267
                     /locus_tag="PK856_01105"
     CDS             3746..4267
                     /locus_tag="PK856_01105"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HRL01001.1"
                     /translation="MVKHQDEGAAKQAFAPIIGVQPRVMVLGSLPGDASLAVGQYYAH
                     PRNQFWRLLSDISGVDLLSLSYADKLQQVQQMPLIVWDVVAEAKRQGSLDSAIREAAI
                     RDIAALQRLHPSVVQVWFNGGQAAKWGIKSLQAAQLDIISVVLPSSSPAHTMAYELKK
                     QQWQQAWNEVFLE"
     gene            4297..5115
                     /locus_tag="PK856_01110"
     CDS             4297..5115
                     /locus_tag="PK856_01110"
                     /inference="COORDINATES: protein motif:HMM:NF020795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwqG family protein"
                     /protein_id="HRL01002.1"
                     /translation="MDKAEMASLFEAFGLTELWSHLLPTLRASIKIKLESQLILQLGD
                     SKIGGMPDLPVTIEWPHAHGKPLAFIAQINLAEVHPLDFENQLPTQGWLYFFYDAANE
                     HWGDVPEDYWHFAVRYFAGEVSELYPAEAPANLASENQFLSAGVALQPQITFPDESSL
                     AWPKVGLSRKQWMALGELEEHVYEMNSGRYNQLLGHSTNLQDGMERQCTRMAKQFFEA
                     DGTKNDWVLLLQLDSNDKECGMMWGDCGVLYFWIRKEDLQQQRFERCWCVMQCF"
     gene            5129..6388
                     /locus_tag="PK856_01115"
     CDS             5129..6388
                     /locus_tag="PK856_01115"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HRL01003.1"
                     /translation="MTDVVALVRQYGIDARAASRVLAAATSAQKNQALLKMADLLEVQ
                     QADVLSANAQDTAAAAANGLDAAMVDRLRISAKSIAQMAEGVRQVAALADPVGEMGDF
                     KLLDNGLQLGKMRAPLGVVGMIYESRPNVTIDAAALCLKSGNACLLRGGSESFHSNQA
                     IAKVIYQALSETGLPGACVRLFETTDRAAVGAMLALSEFIDVVIPRGGKGLVKRITEE
                     ARMPVIKHLDGVCHVFINRDADVEMAVNIAMNAKTSRYGTCNTMETLLVHQDIAATVL
                     PRLQAAYAEKEVELRGCERTLAVLNDIVAATEEDWFTEYLAPVLSIRIVDGLDMAIAH
                     INHYGSHHTDSIVTNDYADIQRFLREVDSASVMVNASTRFADGFEYGLGAEIGISTDK
                     IHVRGPVGLEGLTNQKWIVLGSGQVRG"
     gene            6803..7648
                     /gene="htpX"
                     /locus_tag="PK856_01120"
     CDS             6803..7648
                     /gene="htpX"
                     /locus_tag="PK856_01120"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HRL01004.1"
                     /translation="MKRIFLFIATNMAVMIVIGIILSVLGISGNPNNLAGLLAFSAVV
                     GFTGSIMSLMMSKWMAKKSTGAYVIEQPRNETEAWLLNTVDNQARQLGLKRPEVAIYD
                     APEENAFATGPSKNNSLVAVSTGLLHGMTREEVEAVLAHEMSHVANGDMVTLTLIQGV
                     VNTFVVFLSRLVANMVAGNSDEEEGSSMAYFVVSMVLQVLFGFLASFIVMWFSRQREY
                     RADAGAAKLVGAPKMIAALQRLKGNGESTLPREMNAMGIAGGEKDSLLSTHPTLDNRI
                     AALQK"
     gene            complement(7960..9318)
                     /locus_tag="PK856_01125"
     CDS             complement(7960..9318)
                     /locus_tag="PK856_01125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRL01005.1"
                     /translation="MLRSDDLINLQQQFQFLNTPIVILDTEATGGNLYLDRLTEVALV
                     RFDENGITTYQQLINPEMPISDFIEHLTGISNEMVTEAPRFADIANELLAFLQDAVLV
                     AHNSRFDYQFLRLAFKRCGRHLSMLQLCTVKLSRQLYPQHFKHNLDSIIERFDIHLPQ
                     RHRAMADVAALTQFLSHASTEKAEQWQSAVRKHINPGLFPYWLTHALATQIKALPDEY
                     GVVLIWHAGSKEPKVRVCEHMFQDMCQKFQAKQAQDWFASIERIEGVSAIGPIDAAMI
                     ASRYHNAQKNAFYTVQFVANEHHIIQAKIRPLASERSSHPPFGVFAHPKAAKKALLEW
                     ARKHGFCPKQLDILPQTLAQDAKCPIQLSKQCHGMCAHLNDEATVSQGLQQLVQELPI
                     AGWQAKWAPHISETDPVTGVSKEWTLSRSAVQCEDGSWLWDAAVFQALKDSLRKSKNI
                     HS"
     gene            9450..9526
                     /locus_tag="PK856_01130"
     tRNA            9450..9526
                     /locus_tag="PK856_01130"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9484..9486,aa:Arg,seq:acg)
     gene            9537..9611
                     /locus_tag="PK856_01135"
     tRNA            9537..9611
                     /locus_tag="PK856_01135"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9570..9572,aa:Glu,seq:ttc)
     gene            9787..10317
                     /locus_tag="PK856_01140"
     CDS             9787..10317
                     /locus_tag="PK856_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01006.1"
                     /translation="MTKLAVCAAVAMGLTACASDGGKKPSSKSTEQATTPVATNVKFD
                     SFTEVAYQCRGGSQVKGGKVLALYGIKDGVVGGVQVVYQGSQSPLLLRTTEPKYNEFS
                     SSGRHITWTQNLAAPNQVAVVDAKGLTRLVQNKKADGTMDIGVGIVTDNCKVDKAMTA
                     KLPNMKDDDKVKWLGQ"
     gene            complement(10397..>11655)
                     /gene="ettA"
                     /locus_tag="PK856_01145"
     CDS             complement(10397..>11655)
                     /gene="ettA"
                     /locus_tag="PK856_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HRL01007.1"
                     /translation="DNVEHQLEIAADALRLPDWDAKIEHLSGGEKRRVALCKLLLSKP
                     DMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAVTHDRYFLDNAAEWILELDRGHGIP
                     WKGNYSSWLEQKENRLATESKQEAARIKSMKQELEWVRQNAKGRQAKSKARIARFEEM
                     SSYEYQKRNETQEIFIPTAERLGNEVIEFTNVTKSFGDRVLIDDLSFRIPAGAIVGII
                     GPNGAGKSTLFKMITGKETPDSGEVKVGQTVQLSYVDQSRGNLGNDKTVFDEIAEGRD
                     ILQVGKFETPARAYLGRFNFKGADQSKIVGNLSGGERGRLHLAKTLISGGNVLLLDEP
                     SNDLDVETLRALEDALLEFAGSVMVISHDRWFLDRIATHILAAEGDSKWVFFDGNYHE
                     YEEDKKRRLGEEGAKPKRIRYKPVTR"
BASE COUNT         2878 a         2587 c         3010 g         3080 t
ORIGIN      
        1 acaccacttt gtggctgtta acgatgtctt gtatggtttg ttgcacgctc atgctctgtt
       61 cctaagatga agatttttgc atcaccgccg cgaaaaaacc gtcggtatgg tgggtagcag
      121 gatttaattg caagaattcg cccgtatcga gcgctatttt attacttttc agcaagtcac
      181 tgcaattgac caactgaaaa tcagggtgac tggataaaaa atgttcgact tgtttttggt
      241 tttcttctgc caaaatactg caagtggcgt aaattaaaaa gccgccacgt tttaccaaat
      301 gggcagccga atccaaaatg cgcgtttgca aagtcaccaa ttcgtctatg ctgtcattgc
      361 tttggcggta tttcaaatca ggattgcggc gcaaggtgcc caagcctgaa caaggtgcat
      421 ccacaagtac acggtcggct ttgttccaca attttttcaa acgcaaatcg cgttcgtgat
      481 ccaagcgttg cggggtgatg ttgcccacgc ctgcgcgcgc tatgcgtggc ttgatgttgg
      541 ccaagcgttt ttcagacaca tccatggcgt acaagcggcc tgtattgttc atcatcgctg
      601 ccaaagccaa ggttttgcca cctgcgcctg cacagaaatc caccacgacc tcgccgcgtt
      661 tcgcgccaga aatcaaggcc aataactggc tgccttcgtc ttgtacttct aaaaagccat
      721 ctaagaacaa ggcattttta ttgatggcgg ctttgtcttg aaagcgaatg ccccaaggtg
      781 aaaacggtgt ggctgcggct ttgaaaccat ctgcttcaag ttgagccaat actttttcac
      841 gcttgccttt aatggtgttg acacgtgtat ccagcggcgc cgcttggctt aaagattgac
      901 ctatggcaat gatttcttca tcgctgtact gaccttgcat gcgctctacc aaccattcag
      961 gcaattcact ggcggtattt aagcggcctg taaactcaga tttggcggct ttcaggccgc
     1021 ttaaagcggt gcgttctgct tcattgatga tgtcactcaa agaagcaatg ctgacgccgc
     1081 ggccgatgac caaagcaccc aatgccaaca agcgcgcagg ggctttggcg ccaaagacca
     1141 tgtgtagttt ttgccagtgg cgcaaggctg catacacggt ttcagcgatt tcgtggcggt
     1201 cttggcgacc cagttttttg tggtctttga aaaatttcga caacaccatg tctgccggtg
     1261 agcgaaacag gctcacttcg gcaatcaggc gttcggtatg gatgagttgc ggattgttca
     1321 tttagtattt cttgccgtct aaagtggcgg cagttaagtt aagttgatag gttttaccat
     1381 catcggtata gcgtatcaac gcagggatgt tgccgaggct aggagccaaa ccgtattcca
     1441 tcacatcgcc accacgggta gcgcggtaca tgttcacact caaaggtttg cctttgaatt
     1501 tgacgttttg ggtgcctgcg gcacggatgt tggtggcttc gttatacact tttttaccat
     1561 tggtaaattg tgacacgccg ctgaccttgc cattgtttaa agccaattgc caagccagac
     1621 tgaataagtc catcggcaca ccacgcatcg gggcggtttt gttttcgccc gctttgccat
     1681 aggtgatggt tttgctgcca tggttaaatt tcgcttgggc atacagcttg ccacggcggg
     1741 tatcgctgta gctgtttggc accatggtgt tgccactcat gctgccgctg gagcgaaatt
     1801 gcatgctgta cagcggaatt ttaaacgtgg tgttgatgtt gtaactgctg gcgctgcggc
     1861 cgtaagccat ggtcgcggtc acgccgccat tgccagagta agtcagcgcg gcatttttcg
     1921 gaaaagacac ggtttctgcg taggcgctgc tggccaataa tacagtggtc atgcccaaag
     1981 ctttgatgaa atgttgcatg caatcctcag tttctaataa ttaagccatt attagtctgc
     2041 ttaaaaggcc aaaagtttgc tttcggcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnntct aataattaag ccattattag tctgcttaaa aggccaaaag tttgctttcg
     2221 gctggggcag gctgttggtt gatgaccttg ccgtctttaa tgtgtaaacg gttatttaca
     2281 aaatccgcca ccgcttgtgg gtacaaactg tgttccacaa tcaaagtacg cgtggccaag
     2341 ctgtcggctg tgtcgtcagg caaaatcggc accgcacctt gtgaaatgat ggggccatgg
     2401 tctagctcag gggtgacaaa atgcacgctg caacctgcca atttacagcc cgcatcaatc
     2461 gctgcttggt gggtgtgtaa acctttgaaa gaaggcagta aagaagggtg aatgttgagc
     2521 atgcgatttt ggtagtgcag acagaaatcc gcagttaaaa tgcgcatgaa tcctgccaaa
     2581 accaccaagt caggctgata agcatcgatg gtttgctgta agacggcatc aaatgcttcg
     2641 cggctggtgt aatcgcggtg gttcacaacc tcagtggtga tgccgcgctc gcttgcccac
     2701 gccaagcccg ctgcgtcagg acggttactg atgacagctt tgatgtcagc gcccgcaatg
     2761 cctgcgttca caatggcttg catattgctg ccacggccag agattaaaat caccacattt
     2821 ttattcattg cattgctgtc ctgccgattt tgaagcggcc tgtaaattca tcaagctgcg
     2881 tattatagcc gacagcgaaa ggaaaagaaa aaatggcttt tgacattaaa cgttttttgg
     2941 ttgatggcga taaaaaatgg caatggaaag actacccttg taagtacagc aaagacatcc
     3001 cgaaagctaa agaagaccgc aataaattgg cggcggaatt gtctaccgag ttggatggat
     3061 tgcaagaccg attgtatgga gagggcaaac gcaagctgtt ggtgattttg caaggcatgg
     3121 acacctcggg caaagacggc accatacgcc atgtgtttca aaacgtagac cctctgggcg
     3181 tgtacgtgga agcattcaaa gcgccgagcg aagcggaatt ggcgcgagac tatttgtggc
     3241 gcgtgcatca agtggtgcca aaaaatggtg aaattgccat ttttaaccgc agccattacg
     3301 aagatgtgct ggtgacacgg gtgaaagaat ggatagatgc agataccgaa gccaaacgca
     3361 tagaccacat caatgcgttt gaaaccttgt tgtcagacac aggcacggaa gtggtgaaaa
     3421 tctttttgaa catttccaaa gacgagcagc gtgaacgctt gcaacagcgc attgatgacc
     3481 cgagtaaaaa ttggaaattc aatcttggtg atttggaaga ccgacaattg tgggacaaat
     3541 ttcaaagcca atatgcccgt gtgatgcgca aaacctccag caaaaacgcg ccttggtatg
     3601 tggtgcccgc cgattccaag tccacccgca atattattgt gttgcaaatt ttgctgcacc
     3661 atttgcgcca aatgaaccct cagtatccaa tggtggacac atctgcatgg cccaagcaaa
     3721 tcaaatagca ggaaatgatt gcgacatggt aaagcaccaa gatgagggcg ctgccaaaca
     3781 ggcttttgcg cccatcattg gcgtgcaacc gcgggtgatg gttttgggca gtttgcctgg
     3841 cgatgcgtct ttggcagtcg ggcagtatta cgctcaccca cgcaatcagt tttggcgatt
     3901 gctgagtgac atcagtgggg tagatttgct atcgttgtct tatgcggaca aattgcagca
     3961 agtgcaacag atgccgttaa tcgtgtggga tgtggtggca gaagcgaaac gacaaggcag
     4021 cttggacagt gccatacggg aagctgcaat ccgagacatt gcggctttgc agcgattgca
     4081 tccaagtgtg gtgcaagtgt ggtttaatgg aggacaagct gccaaatggg gaataaaaag
     4141 tttacaggcc gctcaattgg acataatcag cgtggtattg ccttcgagca gccctgctca
     4201 caccatggct tatgaactga aaaagcagca atggcaacaa gcatggaacg aggtatttct
     4261 tgaataactg atgcagtttg atgagaggag tggcgcgtgg acaaagcaga aatggccagt
     4321 ttatttgagg cctttggttt aacagagttg tggagccatt tgcttccgac tttgcgcgcc
     4381 tccattaaaa tcaaactgga gtcgcaactc atcttgcagc tgggtgacag taaaattggt
     4441 ggcatgcctg atttgcctgt caccatcgag tggccgcatg cccatggcaa gcccctggcg
     4501 ttcatagccc aaatcaattt ggcagaagtc catcctttgg attttgaaaa tcaattgccc
     4561 acgcaaggtt ggttgtattt cttttacgat gctgcgaacg agcattgggg cgatgtacct
     4621 gaggattatt ggcattttgc ggtacgctat tttgcaggag aggtcagtga attgtatcct
     4681 gctgaagcac ccgcaaactt ggcttcagaa aaccagtttt tgagtgcagg cgtggctttg
     4741 cagccgcaaa tcacttttcc agacgaaagc agcttggcgt ggcctaaagt gggtttgtca
     4801 cgcaagcaat ggatggcctt gggcgagctg gaagaacatg tgtatgaaat gaattcaggc
     4861 cgctataacc agcttttggg ccactccacc aatctgcaag atggcatgga gcgccaatgt
     4921 acccgcatgg ccaagcagtt ttttgaggct gatggcacca aaaatgactg ggtgttgttg
     4981 ctgcaattgg acagcaacga caaagagtgt ggcatgatgt ggggcgattg tggcgtcctg
     5041 tatttttgga tacgcaaaga agatttgcag caacagcgat ttgagcgttg ttggtgcgtg
     5101 atgcaatgtt tttagtgaag ggtagaacat gaccgatgtg gtggcgctgg tgcgtcaata
     5161 cggtatcgat gccagagccg catctcgggt gttggcagcg gcgacaagtg cgcaaaaaaa
     5221 tcaggcattg ttgaaaatgg ccgatttgct cgaggtgcaa caagccgatg ttttgagcgc
     5281 gaatgcccaa gacacggcag cggccgcagc caatggtttg gatgcggcca tggtagaccg
     5341 cttgcgcatt tctgccaaat ccatcgcaca gatggccgag ggcgtacgac aagtggctgc
     5401 tttggccgat cctgtgggcg aaatgggcga tttcaagctg ctagacaatg gtttgcaatt
     5461 gggcaaaatg cgcgcgcctt tgggtgtggt gggcatgatt tacgaatcgc gccccaatgt
     5521 caccattgat gcggcggctt tgtgtttgaa atctggcaat gcctgcttgc tccgtggcgg
     5581 ctcagaatcg tttcacagca accaagccat cgccaaagtc atctaccaag ccttgagtga
     5641 aacaggcttg cccggagctt gtgtgcgttt gtttgaaacc accgaccgtg cggccgtagg
     5701 cgcaatgttg gctttgagtg aattcattga cgtggtgata ccgcgcggtg gcaaaggctt
     5761 ggtgaaacgc attaccgaag aagcgcgcat gcctgtcatc aagcatttgg acggtgtgtg
     5821 ccatgtgttc atcaaccgtg atgccgatgt ggaaatggcg gtgaacattg ccatgaatgc
     5881 caaaacctcg cgctacggca catgcaacac catggaaacc ttgttggtgc accaagacat
     5941 tgccgccacg gtattgccac gtttacaggc cgcttatgcg gaaaaagaag tggaattgcg
     6001 tggttgtgag cgcacacttg cagtgttaaa cgacattgta gctgccacag aagaagattg
     6061 gtttaccgag tatttggcgc ctgttttgtc catacgcatc gtcgatggtt tggacatggc
     6121 gattgctcac atcaaccatt acggcagcca ccataccgac agtatcgtca ccaacgatta
     6181 tgccgacatt caacgcttct tgcgagaggt cgattcagcc agtgtgatgg tgaacgcttc
     6241 tacgcggttt gccgatggct ttgaatacgg cttgggtgcg gaaattggca tctctaccga
     6301 caaaatccat gtgcgcggcc cagtgggatt ggaaggcttg accaatcaaa aatggattgt
     6361 attgggcagc ggtcaagtgc gcggttaaat ctcattcgtt ttaaaaagcc atatttgcct
     6421 gcaaatatgg cttttttgtt atgagcctgt ttttgaatag cagcgtgtac aagcggcctg
     6481 taggtattgt gtagcagtta caggccgcat gatggttaat accatgatta aaatagcata
     6541 tgcactcacc accacagtgt ctggcctgct tgagctgcag gcatttgcga gtgtgtgcat
     6601 cagggatttt atgcatgtct ctaggggttt agggacttgt aaaaacgagg ctttaaatta
     6661 gagtgggcga ttactttaaa tattcagtat taaacggttg ctatgtgtat gtaaaattgc
     6721 ttaaaaattc tgaaatttga aaaaaatgcc ttaatattat tcacgttgaa acacacttca
     6781 catattttaa aaaagggaac tcatgaagcg gatttttttg tttattgcca ccaatatggc
     6841 ggtgatgata gtcatcggta ttattttgtc ggtattgggc atcagtggta accccaataa
     6901 tctggcgggc ttgttggctt tttcagcagt ggtgggtttt acaggctcca tcatgtcttt
     6961 gatgatgtcc aaatggatgg caaaaaaatc cacgggcgct tatgtgatag aacagccacg
     7021 caatgaaacc gaagcatggt tattgaatac ggtggacaat caagcgcgcc aattgggttt
     7081 gaagcgtcct gaagtggcaa tttacgatgc acctgaagag aatgcctttg ccacaggccc
     7141 tagcaaaaac aattcattgg tggcggtgag cacaggtttg ttgcatggca tgacgcgcga
     7201 agaagtggag gcagtgttgg cacatgaaat gtcgcacgtg gccaatggtg acatggtgac
     7261 tttgaccttg attcaaggtg tggtgaacac gtttgtggtg ttcttgtcgc gcttggttgc
     7321 caatatggtg gcgggcaaca gcgatgaaga agagggcagc tctatggctt actttgtggt
     7381 gagcatggtg ttgcaagtgt tgtttggctt cttggccagc tttattgtga tgtggttcag
     7441 ccgccaacgc gagtaccgtg ccgatgctgg tgcagccaaa ttggtgggtg cgcccaaaat
     7501 gattgcggcc ttgcaacgct taaaaggcaa tggcgagagc actttgccac gagaaatgaa
     7561 tgccatgggc attgccggtg gtgaaaaaga ctctttgtta agcacccatc ctactttgga
     7621 caaccgcatt gcagctttgc aaaaataagc cttcaaactg aatgctgaaa gcggcctgta
     7681 acttacaggc cgctttttta tgggattctg ggtcatggat gattagatga aatgggtgtg
     7741 ttgatgttgc caagcaatat tttcttagtg ttgggtaaat tgcagatagt gcgatgagat
     7801 aggtcatgag cggggaggta tggctgatgc caacatccaa tattattggt gtgcaattgt
     7861 aagagccatt tcatcatggc tgtattcaat cagagaggcg gcctgtaaag cttcaatatt
     7921 tacaggccgc tgatgatttc caaatggctg actacacatt taactgtgaa tatttttgga
     7981 tttacgcaag ctgtctttca gtgcttgaaa taccgccgca tcccaaagcc atgaaccatc
     8041 ttcacattgc accgctgagc gcgatagtgt ccactctttg ctcactcccg ttacgggatc
     8101 ggtttcactg atgtgcggcg cccatttggc ttgccagccg gcaataggca gctcttgtac
     8161 cagttgttgc aaaccttgtg acaccgttgc ctcatcattc aaatgagcgc acatgccatg
     8221 gcattgcttg ctcagttgta tcgggcattt ggcgtcttgg gccaaagtct gcggcaatat
     8281 atccaattgc ttggggcaaa acccatgttt gcgcgcccat tctaacaagg cttttttggc
     8341 agctttggga tgggcaaaaa ccccaaatgg cgggtgcgaa ctgcgttcac tggccaaagg
     8401 gcgtattttg gcttggataa tgtggtgttc attggccaca aactgaaccg tgtaaaacgc
     8461 atttttttgg gcattgtggt agcggctggc tatcatggcc gcatcaatgg gccctatggc
     8521 actgacgcct tcaatgcgct ctatgctggc aaaccagtct tgcgcttgct tggcttgaaa
     8581 tttttgacac atgtcttgaa acatgtgttc acacacgcgc accttaggct ctttactgcc
     8641 cgcatgccaa atcaacacca cgccatattc gtcaggcaaa gccttaattt gtgttgccaa
     8701 ggcgtgggtg agccagtatg gaaataagcc aggattgatg tgtttgcgca cagcgctttg
     8761 ccactgttcg gctttttctg tgctggcatg gctgagaaat tgcgtcaatg cggccacgtc
     8821 tgccatggcg cggtggcgtt ggggtaggtg gatgtcaaaa cgctcgatga tgctgtcaag
     8881 attgtgtttg aaatgctgcg ggtagagctg gcgcgacaat ttcaccgtac acaattgcaa
     8941 catgctcaag tgacggccac aacgcttgaa ggccaaacgc aaaaactggt aatcaaagcg
     9001 gctgttgtgc gctaccaata ccgcatcttg taaaaaggcc aacaattcat tggcgatgtc
     9061 ggcaaagcgt ggtgcttctg tcaccatctc attgctgatg cccgttaaat gctcaataaa
     9121 atcactgatg ggcatttctg ggttaatcaa ttgctggtaa gtggtgatgc cgttttcatc
     9181 gaaacgaacc aaagcgactt cggtgagtcg gtccaagtac aagttgccac ctgtggcttc
     9241 ggtgtccaag atgacgatgg gggtattgag aaattgaaac tgttgctgca gattgatcaa
     9301 atcatcagag cgtaacatgg ggcgctgcca aaaacaaaaa cacgccattt tagcgctaaa
     9361 atcagttgca taccgaattt tatgaatatt tttataaaaa gcacttgacg catacggggt
     9421 ctcttcttat aatgctttct ctttcagatg cacccgtagc tcagttggat agagtatctg
     9481 gctacgaacc agagggtcgg gcgttcgaat cgctccgggt gcaccactga agatacgcgc
     9541 tcatcgtcta gcggttagga cactgccctt tcacggcggc aaccggggtt cgattccccg
     9601 tgggcgtacc aattttaaaa gccagcttgc aaaagctggc tttttcatat tgggttcaag
     9661 cagtttgttt tcatgctatt catgtcacga tgatttttgc atgtcttaac ttggaactta
     9721 tatctaagca tggtaacctg attttacttt tttaagcttg agtaggatga ctttatgcaa
     9781 gtaaacatga ctaaattggc ggtatgtgcg gcggtggcaa tgggtttgac agcttgtgct
     9841 tccgatggtg gtaaaaaacc ttcttctaaa tctaccgaac aagccaccac tcctgtggct
     9901 accaatgtga aatttgattc gtttactgaa gtagcgtacc aatgcagggg cggtagccaa
     9961 gtcaaaggtg gtaaagtatt ggctttgtat ggcattaaag acggtgtggt tggcggcgtg
    10021 caagtggtgt accaaggctc acaatcgcca ttgctgttaa gaaccactga gccgaaatac
    10081 aatgaatttt cttctagcgg ccgccacatc acttggaccc aaaacctggc tgctccaaac
    10141 caagtggctg tagtggatgc aaaaggtttg acgcgtttgg ttcagaataa aaaagctgat
    10201 ggcactatgg acattggcgt gggcattgtg actgacaact gtaaagtaga taaagccatg
    10261 actgccaagt tgcctaatat gaaagatgac gacaaagtga aatggttggg acaataattt
    10321 aaattgttcc atagatatta aagcggcttg taaatgattt acaggccgct tttgttactg
    10381 ttgaagttac accagattaa cgtgtcacag gtttgtagcg tatgcgtttc ggtttcgcac
    10441 cttcttcgcc caaacggcgt tttttgtctt cttcatactc atggtagtta ccatcaaaga
    10501 acacccattt tgaatcgcct tcagctgcca aaatgtgtgt ggcgatgcgg tctaagaacc
    10561 agcgatcatg tgaaatcacc atcactgagc cagcaaattc caacaaggca tcttcaagtg
    10621 cacgcaaggt ttccacgtcc aagtcgttag acggttcgtc caagagcagt acgttaccac
    10681 cagaaatcag ggttttggcc aaatgcaagc ggccacgttc accacccgaa agattaccga
    10741 cgattttgct ttggtctgcg cctttgaagt tgaagcggcc caaatacgca cgggcagggg
    10801 tttcaaattt acccacttgt aagatgtcac ggccttcagc gatttcatca aaaacggttt
    10861 tgtcattgcc caaattacca cggctttggt ctacataaga caattgtacg gtttgtccta
    10921 ctttcacttc accagagtct ggggtttctt tgcctgtgat cattttgaac aatgtggatt
    10981 tacccgcacc attcggcccg atgatgccga caatggcacc agcgggaatg cggaagctca
    11041 aatcatcaat caatacacgg tcaccaaacg attttgttac attggtgaat tcaatcactt
    11101 cattgcccaa acgctcagcg gtagggatga aaatctcttg ggtttcattg cgcttttggt
    11161 attcgtaact cgacatttct tcaaaacggg caatacgcgc tttggacttg gcttggcggc
    11221 ctttggcgtt ttggcgtacc cattccaatt cttgtttcat cgatttgatg cgcgcggctt
    11281 cttgttttga ttcggtggcc aagcggtttt ctttttgctc caaccaagac gagtaattgc
    11341 ctttccaggg aatgccatga ccacggtcca gttccaaaat ccactcggca gcattgtcta
    11401 ggaagtagcg gtcatgcgtt actgcaacca cggtgcctgg gaagcgcacc aagaattgct
    11461 ctaaccactc tactgattcg gcatccagat ggttggtggg ttcgtccaaa agcaacatgt
    11521 caggcttaga cagcaacaat ttgcacaagg ctacacggcg tttttcaccg ccagacaggt
    11581 gttcgatttt ggcatcccaa tcaggcaagc gcagcgcatc ggcggcgatt tccaattggt
    11641 gctccacatt gtcgc
//
LOCUS       DAOMVK010000014        10681 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_1489_length_10681_cov_3.285244, whole genome shotgun sequence.
ACCESSION   DAOMVK010000014 DAOMVK010000000
VERSION     DAOMVK010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 10681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10681
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1489_length_10681_cov_3.285244"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..970
                     /gene="cysM"
                     /locus_tag="PK856_01150"
     CDS             59..970
                     /gene="cysM"
                     /locus_tag="PK856_01150"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HRL01008.1"
                     /translation="MAQNNVFAHTDYLLDHFVGGTPLVRLQRLSQPEHGTVLAKLEGN
                     NPAGSVKDRPAYNMIMQAEKRGDIQPGDTLIEATSGNTGIALAMVAAMRGYKMQLIMP
                     DNMSQERKDAMRVYGAELIEVTKEQGMEGARDLALQLQAAGKGIVLDQFANPDNLEAH
                     YLTTGPELWQQTQGKITHFVSSMGTTGTIMGVSKYLKEQNPDIQIVGLQPAEGSSIPG
                     IRRWPQEYLPKIFDASRVDVIMDVEQSDAEATMRALATQEGIAAGVSSGGAAWAALQI
                     AQQHPDAVIVCIICDRADRYLSTGVFS"
     gene            1130..1678
                     /gene="lspA"
                     /locus_tag="PK856_01155"
     CDS             1130..1678
                     /gene="lspA"
                     /locus_tag="PK856_01155"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRL01009.1"
                     /translation="MHNEKPPVALGKYLIVSVVAILLDQLTKWLVIKNIPAPVYDAQG
                     YFLGATRINVIPDFFDLTHVYNPGAAFSFLANAGGWQVVFFSALAFIISGWLLWSIRK
                     AQFACWGNWGAAAIVGGALGNVVDRFVHGHVIDFLLFYVEPHYYPAFNIADSFICVGA
                     VMLVIDGFAQAKKDKQNKQVSV"
     gene            1689..2618
                     /gene="ispH"
                     /locus_tag="PK856_01160"
                     /gene_synonym="lytB"
     CDS             1689..2618
                     /gene="ispH"
                     /locus_tag="PK856_01160"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRL01010.1"
                     /translation="MPQPILLANPRGFCAGVDRAISIVERALEEYGAPIYVRHEVVHN
                     KFVVDDLRNKGAIFIEELNDVPEGSTLIYSAHGVSLAVQEEAIRRGFRIFDATCPLVT
                     KVHKQVQRLNEDGYQIIMIGHKGHPEVEGTMGQVREGGMLLVEVVEDVANLVVDDVEK
                     LAHVSQTTLSVDETFDIIEALKARFPHIKSPNKEDICYATTNRQEAVKKLAADCDIVI
                     VVGSPNSSNSNRLREVAALRGVDAYMVDNASFLQEEWFAGKNHVGVTAGASAPELLVQ
                     EVINQIQAWGHGSIEELNGVEESIVFVLPKELR"
     gene            2838..3815
                     /locus_tag="PK856_01165"
     CDS             2838..3815
                     /locus_tag="PK856_01165"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRL01011.1"
                     /translation="MKLKQFYTGGALALGLAVAGSAWAQTSTAAVTQGAQAQDNRLVL
                     LGTQGGPRVGVKRANPANLIVINGKKFVVDAGYGVSMQMVKAGYRLQDLDYIFITHMH
                     SDHTLEYGNLFYNAWVTGGLKKPVQVYGPTGMKNMTEAFFKTNEIDITTRMADEDRAD
                     PRKLIQVHEIQKLNPVFNRDGIKVSAIKSIHPPFDETYSFKFEFNGKTVVFSGDTAYN
                     KDMVAFAKGADYLVHEVQYEDGIRRLAKKLAGDKQSAEQQYQHFKGSHTNTEELGRLA
                     QAAGVKNLVLTHYVPGDDPLITDVMWEEGVKQFYRGNVVVGKDLTSIDL"
     gene            3911..5005
                     /locus_tag="PK856_01170"
     CDS             3911..5005
                     /locus_tag="PK856_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRL01012.1"
                     /translation="MQFKFIFGMGLCACLLHANADVVIYGQIKGGIGSNHNTDGGSIT
                     KIEDYRSKIGFKGQEKLGDATDVVWQIENAISIAGDSTGKQNSWNSFDIFVGVKNNDW
                     GTMKLGYMADGMLGVPNQKMQVDPWESNTDINDPKGMKIFTRGEKRLTGIRYDMPTVH
                     GVDFNITHQLADSSQTEAFDAETTVNQATVLAAAYKHGPWYAKGAAGLYKHQFVEHGD
                     IEDAQLYRLLAGYDDKKTMVGVGFQYMDGFKGLWGRGMATVDKPVTPASTTREAIVTA
                     SHKIGRLTPRVAYAHGWDEKQADKGQIDNTSYDQVIVGANYAFSKTTTGLLTAGWIRK
                     PMAYTTLSDEQLSADKQSIYGLGVGLKTVF"
     gene            complement(5059..6252)
                     /locus_tag="PK856_01175"
     CDS             complement(5059..6252)
                     /locus_tag="PK856_01175"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL01013.1"
                     /translation="MQQHASSSSLFSNKTLWILMLGTFAMGNAKFSIMPLVPVIAQDF
                     HTTEDAVLQTILAYFWGGLIAAPLIAIFCRHWAKHHILVMLSLWCFAGNLLSSFATNV
                     DMLYWLRWFSGIPHAVFLAFACLLAGEMAPPQQRGRYMGWALLGLAVATMVAVPFNAW
                     IGNSYGWQIAFRFVALVDLLIVLLIHDLIPATKPVVQRPPLAAQLRQLRQPAIWLLFG
                     IGLCLITGMTVIWSFSLPLFTQVAHLPAHLSTVLLVLLGLGFVASQFMGGYLADRHAN
                     TAVLLCVLWALMAAAILWVFLLDYRLALLGAFFISTISMINVVVQTRLLHMPHIPLYL
                     VFCLYNACIQLGNFIGSLIAHAISQSTWPMSSVIIISLLCSVLAGLLYWAMQQQQNRL
                     TVPAA"
     gene            6757..7590
                     /locus_tag="PK856_01180"
     CDS             6757..7590
                     /locus_tag="PK856_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRL01014.1"
                     /translation="MMKTVNKYLCSVVGVVVLGMVLSSCAQKLFYYPDAIEHGHGPAQ
                     YQQPYQDLVFNSHDGTKLHGWFVPSRLHANSKLAKGTVVHFHGNAQNISSHYALVSWL
                     PDQGYNVFTFDYRGFGSSEGHVGFAGIVDDADAALDYVRKLPGVDEQRLYVVAQSIGG
                     NKAIAAIGRGNRAGIRAMVVDSTFYGYDAIANAKFSGASVLVNDQLSAHRYIGAISPI
                     PLLMLHGTADNVIPYQQGQRLFTQAREPKQFITIPNGTHITALANPVYQQQVLQWLNQ
                     Y"
     gene            complement(7623..8831)
                     /locus_tag="PK856_01185"
     CDS             complement(7623..8831)
                     /locus_tag="PK856_01185"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL01015.1"
                     /translation="MPPSADANDTNASPMRCLWVLMFGAFAMGNAKFSIFPLVPAIAA
                     DLNSSPSEILHAVFAYLWGGLIAAPIMAIAFRNWSKPRILTLLSVWCFLGNLLTSFAT
                     SADMLYWLRWFSGIPHAAFLAFSTLLIAEIAPPNKRGRYLGLSLVGISLSTLFVVPFN
                     TWIGLDYGWQISFRFVALLDLLIFLLIHDLLPQSRVPAIKQTISMQLHALKNRRLWLL
                     FAVGLCILISTSAAWTYSIAWFNLHPHPSLNLRILVLIILGIGFIVGQIAGGWAADHQ
                     IDRYIGFNTLYTMFAAIFALSFLFDYRLGLVGVFLISIAFSLVNVMMQIRLLEYPPAA
                     LYMVLCLFNACIQLGNFSGSALAKFAAQNFNTPTTTLSILLAALAATAAILWFWIIKH
                     PTSPQKQQAS"
     gene            complement(9283..9657)
                     /locus_tag="PK856_01190"
     CDS             complement(9283..9657)
                     /locus_tag="PK856_01190"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRL01016.1"
                     /translation="MNLAKVSALTDVSPRMLRYYEELGLIAPKRGDNNYRSYSAHDIE
                     CINKIKILNDAGMNLKAIHWLLPCFDLDSQSFKLCRIANELVEQELVSIEQKLEQLQQ
                     SQSLLQSFIAKSKQLQAETPPI"
     gene            9804..>10681
                     /locus_tag="PK856_01195"
     CDS             9804..>10681
                     /locus_tag="PK856_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL01017.1"
                     /translation="MTTDKQSSSSLWLMVLAGSLILMITMGVRNSLGLYIQPISLSTS
                     VNIASISLALAVGQFLWGAIQPIAGALADRYGSRPILLIGMVILILGLVIAPMWTSSF
                     GLMVSLGVLSAIGSGIGSFSVLMGAVAQHITPQMRGNATGILNAGGSFGQVVFSPLIQ
                     VLIKSVGWMQSLFVVAAITLSTIPLIFAATKPKSAASTVAQDNNGIGLKQTVLQALQN
                     RDYLLLHLGFFTCGFHIAFLVTHLPGEVAICGLNAKVAGWSLAIIGLANVAGSLLAGR
                     WVSRYRSKYILFWMYA"
BASE COUNT         2899 a         2395 c         2807 g         2580 t
ORIGIN      
        1 ggtacaataa cggcttcttg cggcgagaag ccgctcattg cagttgagaa agacaaacat
       61 ggcacaaaac aatgtgtttg cacataccga ttatttatta gaccattttg tgggtggcac
      121 gccgttggtg cgtttgcaac gcttaagcca gcctgaacac ggcacagtat tggccaagct
      181 tgaaggcaac aatcctgcgg gctcagtcaa agaccgtcct gcttacaaca tgattatgca
      241 agcggaaaag cgcggcgaca tccagcctgg cgacaccttg attgaggcga cttctggcaa
      301 cacaggcatt gccttggcga tggtggcggc gatgcgtggc tacaaaatgc agctcatcat
      361 gcccgataac atgagccaag agcgcaaaga cgcgatgcgc gtgtacggtg cggaattgat
      421 tgaagtcacc aaagaacaag gcatggaagg cgcgcgcgat ttggctttgc aattacaggc
      481 cgctggcaaa ggcatcgtgc tcgaccaatt tgccaatcct gataatttgg aagcgcatta
      541 cctcaccaca ggccccgaat tgtggcaaca aacccaaggc aaaatcaccc attttgtcag
      601 ctccatgggc accacaggca cgattatggg cgtgtcgaaa tacctgaaag agcaaaaccc
      661 tgacatccaa atcgtcggtt tgcaacccgc agaaggctca agcattccag gcatacgccg
      721 ctggccgcaa gaatatctgc ccaaaatctt cgacgcctcg cgcgtggacg tgattatgga
      781 tgtggaacaa agcgatgccg aagccaccat gcgtgccttg gccacacaag aaggcattgc
      841 cgcaggcgtg tcttcaggtg gcgctgcatg ggcagctttg caaattgccc aacaacatcc
      901 cgatgcggtg atagtgtgca tcatctgtga cagagccgac cgctatttgt cgactggggt
      961 gttttcctaa aacttacagg ccgcttttaa gcggcctgta aatgcggcaa tacaatatat
     1021 ccgcttttac aattgcaatt tcagccatca tgcccatttg caaactcagt cgctcaagct
     1081 gccacaggtg tgcgacaata cgcccgtttt gatgaaagtt atttttgcta tgcacaatga
     1141 aaaaccgccc gttgctttgg gcaaatacct gattgtttcg gtggtggcga ttttgctcga
     1201 ccaattgacc aaatggctgg tgattaaaaa catccccgcg cctgtgtacg atgcacaagg
     1261 ctattttttg ggcgcgacgc gcatcaatgt catccccgat ttctttgatt tgacccatgt
     1321 gtacaaccct ggcgcggcgt tcagcttttt ggccaatgca ggcggctggc aagtggtgtt
     1381 tttctcggct ttggccttca tcatcagcgg ctggttgctg tggtcgatac gcaaagccca
     1441 gtttgcctgt tggggcaatt ggggcgcggc ggcaatcgtc ggcggtgctt tgggcaatgt
     1501 ggtcgaccgt tttgtgcatg gccatgtcat cgactttttg ttgttttatg tggagccgca
     1561 ttactaccca gcgtttaaca ttgccgacag cttcatctgc gtgggtgcgg tgatgttggt
     1621 gatagacggt tttgcgcaag cgaaaaaaga caaacaaaac aaacaggttt cagtttaagg
     1681 acacagccat gccacaaccg attttacttg ccaatccgcg cggcttttgt gcgggcgtag
     1741 accgagccat cagcatagtg gagcgtgcct tagaagaata tggcgcgccg atttatgtgc
     1801 gccatgaagt agtgcacaac aaatttgtgg tcgatgattt gcgcaacaaa ggtgcaattt
     1861 tcattgaaga attgaacgac gtgcccgaag gctcaacctt gatttattcg gcacacggtg
     1921 tgtctttggc ggtgcaagaa gaagcgatac gtcgtggctt ccgcattttt gacgccactt
     1981 gcccattggt gaccaaagtg cacaaacaag tgcagcgcct gaatgaagac ggctaccaaa
     2041 tcatcatgat cggccacaaa ggccacccag aagtggaagg cacgatgggg caagtgcgcg
     2101 aaggtggcat gttgttggtg gaagtggtgg aagacgtggc caatttggtg gtagacgatg
     2161 tggaaaagct tgcccacgtt agccaaacca cgctgtctgt ggacgaaacc ttcgacatca
     2221 tcgaggcctt aaaagcccgt ttcccccaca tcaaaagccc gaacaaagaa gacatttgct
     2281 acgccaccac caatcgccaa gaagcagtga aaaaattggc ggccgattgt gacattgtga
     2341 ttgtggtggg ctcgcccaac tcatctaaca gcaaccgctt gcgcgaagtg gctgctttgc
     2401 gcggcgtcga tgcttatatg gtagacaatg cgagcttctt gcaagaagaa tggtttgcag
     2461 gcaaaaacca cgttggcgtg accgcaggcg catccgcacc cgaattgttg gtgcaagaag
     2521 tcatcaacca aatccaagct tggggccacg gcagcattga agaactcaat ggtgtggaag
     2581 aaagcatcgt gtttgtgcta ccgaaagagt tgcgttaaac aaacatgcga ttgcacacaa
     2641 tcgcagcaat caaataaagc ggcctgtaag tgtttacagg ccgctttatt tcgttttaag
     2701 cacctatgca attgaattga tggcagatgt gtgtgattgt agataagagt tatgccgatt
     2761 ttaatagcag aaaatagctg gaatgcgttt atatttctcc attcatacta aaaatacaca
     2821 tgatggaaga gaagacgatg aagctaaaac aattttacac aggtggtgca ttggctttgg
     2881 gcttggctgt tgctggctct gcttgggcgc aaacgagtac tgctgcggtt acgcaaggtg
     2941 cgcaagcaca agacaatcgc ttggtattgt taggcacaca aggtggcccg cgtgtgggcg
     3001 tgaaacgcgc caatcctgcc aatttgattg tcatcaatgg caaaaaattt gtggtggatg
     3061 cgggctatgg ggtgtcgatg caaatggtga aagcaggcta ccgcttgcaa gatttggatt
     3121 acattttcat tacccacatg cattctgacc atactttgga atacggcaat ttgttttaca
     3181 acgcttgggt gacgggaggt ttgaaaaagc ctgtgcaagt gtatggccca acaggcatga
     3241 aaaacatgac tgaggcgttt ttcaaaacca atgaaattga cattaccaca cgcatggccg
     3301 atgaagaccg tgccgaccca cgcaaactca ttcaagtgca tgaaattcaa aagctgaatc
     3361 cagtgttcaa tcgagatggc atcaaagtca gcgccatcaa atccatacac ccaccttttg
     3421 acgaaaccta cagttttaaa tttgaattca atggcaaaac agtggtgttc tcaggcgaca
     3481 cggcttacaa caaagacatg gtggcgtttg ccaaaggcgc tgattatttg gtgcatgagg
     3541 tgcaatacga agatggcata cgccgtttgg ccaaaaaatt ggcaggcgac aagcaatcgg
     3601 ctgagcagca ataccaacac tttaaaggct cgcacaccaa taccgaagaa ttgggccgtt
     3661 tggcacaggc tgcgggcgtg aaaaatttgg tgttaacgca ttatgtgcca ggtgatgatc
     3721 cgttgattac cgatgtgatg tgggaagagg gcgtgaagca gttttaccga ggcaatgtgg
     3781 tggtgggcaa agacttgacc agcattgact tgtaacactg ctcagacaga aacacatcct
     3841 ctttttaatc ctcaatgggg tttacaggcc gcttgaatgg cttgtttgcc ctgtgcttgg
     3901 gaatgtacac atgcaattca aatttatttt cggcatgggc ttatgcgcat gtctgcttca
     3961 tgccaacgca gatgtggtga tttacggcca aatcaaaggc ggcatcggca gcaatcacaa
     4021 tactgatggt ggttccatca ccaaaataga agactaccgc tcaaaaattg gctttaaggg
     4081 acaggaaaag ttgggcgacg ccaccgatgt ggtgtggcaa attgaaaacg ccatttcgat
     4141 agcaggcgac agcacaggta agcaaaacag ctggaattca ttcgatattt ttgtgggggt
     4201 gaaaaacaat gattggggca cgatgaagct cggttacatg gccgatggca tgttgggtgt
     4261 gcccaatcaa aaaatgcaag tagacccatg ggaatcaaac accgacatca acgaccccaa
     4321 aggcatgaaa atctttaccc ggggggaaaa gcggttaaca ggcatacgct acgacatgcc
     4381 tacggtacac ggcgtggatt tcaacattac ccaccagttg gcggacagtt cacaaacaga
     4441 ggcatttgat gccgaaacca cggtgaacca agccaccgtg cttgccgcag cttacaaaca
     4501 cggaccttgg tatgccaaag gtgccgctgg tttgtacaaa caccaatttg tagaacacgg
     4561 cgacattgag gacgcacagt tgtatcgcct attggcggga tacgatgaca agaaaaccat
     4621 ggtaggcgtg ggttttcaat acatggatgg attcaaaggt ttgtgggggc gaggcatggc
     4681 cacggttgat aagcctgtga caccagccag caccacacga gaagccattg ttaccgcctc
     4741 gcacaaaatc ggccgattga ccccacgtgt ggcctatgcg cacggatggg atgaaaagca
     4801 agcagataag gggcagattg acaatacctc ttacgaccaa gtcatcgtgg gcgccaatta
     4861 cgcgttttca aaaaccacga caggtttgtt gacggcgggt tggatacgca aacccatggc
     4921 ttataccacg ttgtcagatg aacaattaag tgctgacaaa caatcgattt atggattggg
     4981 cgtgggttta aaaaccgtat tttgatgtgt catcaagcat gtaaaagcgg cctgtaaatg
     5041 agtatacagg ccgttttatt atgcagcggg gactgtgagg cgattttgtt gctgttgcat
     5101 ggcccaatac agcaagcccg ccaatacact gcacagcaaa gaaatgatga tgacgctgct
     5161 catgggccaa gtggattgtg agatggcgtg ggcaatcaaa gagccgatga agttgcccaa
     5221 ttgtatgcag gcattgtaca aacaaaacac cagatacaaa ggtatgtgtg gcatgtgcag
     5281 caagcgggtt tgcaccacga cattgatcat ggaaatggtg ctgatgaaaa atgcgcccaa
     5341 taaagccaag cggtagtcca acaaaaagac ccacaagatg gctgcggcca tcaaagccca
     5401 cagcacacac agaagaacag cggtgttggc atggcggtcg gccaaatacc cacccatgaa
     5461 ttggctggca acaaaaccca atcccaacaa taccaacagc actgtgctca aatgcgcagg
     5521 caggtgagcg acttgggtaa acaaaggcaa gctgaatgac cagatgacgg tcatgcctgt
     5581 aatgaggcag aggccaatgc caaacagcaa ccagatggct ggttggcgta attggcgcag
     5641 ctgtgcggcc aaaggtggac gctgaacaac tggtttggtg gcaggaatca agtcatgtat
     5701 cagaagaacg atcagcaaat caacgagcgc tacaaaccga aatgcaattt gccaaccata
     5761 gctgttgcct atccaagcat tgaacggtac ggccaccatt gtggccacag ccaagcccag
     5821 caaagcccaa cccatgtaac gcccacgttg ttgtggtggt gccatttcac ctgccagcaa
     5881 acaggcaaag gccaaaaata ccgcatgggg gatgccactg aaccagcgca gccaatacag
     5941 catgtccaca tttgttgcga atgaactgag taaattgccc gcaaaacacc ataagctgag
     6001 catgaccaaa atgtggtgtt tggcccagtg gcggcaaaaa atggcaatca acggtgctgc
     6061 aatcagccca ccccagaagt aagccaagat ggtttgtaag accgcatctt cggtagtgtg
     6121 aaagtcttgg gcgatgacgg gcaccaacgg catgatggag aatttggcat tgcccatggc
     6181 gaacgtgccc agcatcaata tccacagggt tttgttggag aagagtgagg aagaagaggc
     6241 gtgttgctgc atgtggtttc caaatgaatg tgttcaactt gggtgtggaa tacaaaaatt
     6301 gttttaacta cccattaata gaatagccac acttatgtca tgagcgtatt ttaacctttt
     6361 ttgacacaag ttggcacaat ggcttgatcg tcaagccatt ttataagtat tggaggtgtg
     6421 ttttgttggg ttgagagcat tgcaaaccga ttgttggtgt aggtagaaaa gtttgaagct
     6481 accaataatg cacattgtct cacaattaaa aattgctcca tgttgggttg ggtcaaggtt
     6541 ggtgttaatt gtgagagtaa ggacttaatt tcaatgacaa actgagccga tatcctttca
     6601 tattcagtta aaatgtttgt tacacaacaa aaaagcggcc tgtaaacatt tacaggccgc
     6661 ttttttaacc tgaagtggcg aaatatgctg ccataagcct gcacattgca tgaatgggtg
     6721 ttgctggttt tatgatgggc tagaggaggc cagttgatga tgaaaacagt caataaatac
     6781 ttgtgcagtg tggtgggtgt ggtggtgttg ggaatggtgc tcagttcttg tgcgcagaag
     6841 ctgttttact atccagatgc catcgaacat ggccatggcc ctgcgcaata tcaacagcct
     6901 taccaagatt tggttttcaa cagccatgat ggcacgaagt tgcatggttg gtttgtgcca
     6961 tcgcgcttgc atgccaattc gaaattggcc aaaggaaccg tggtgcattt tcatggcaat
     7021 gcacaaaata tcagcagcca ctatgctttg gtgagttggt tacctgacca aggttacaat
     7081 gtgttcactt ttgattaccg tgggtttggc agctcggaag gacacgttgg ctttgcgggc
     7141 atcgtggatg atgccgatgc ggctttggat tatgttcgga agctgcctgg ggtggacgag
     7201 cagcgtttgt atgtggtggc acagtctatt ggtggcaaca aagccattgc tgccataggc
     7261 cgaggcaata gggcggggat aagggcaatg gtggtggatt caaccttcta tggatacgat
     7321 gccattgcca atgccaagtt ttcaggggcg agtgtgttgg tgaacgatca attgagtgca
     7381 catcgctata ttggcgccat cagcccgatt ccattgttga tgctgcatgg tacggcggat
     7441 aatgtgattc cttatcaaca ggggcaacgt ctgttcacac aggcgcgtga gcccaaacaa
     7501 ttcattacca tcccgaatgg tactcacatc acggcattgg caaatcccgt ctaccaacag
     7561 caggttttgc agtggttaaa tcaatattga atgtgattta aagctttaag gcggcctgta
     7621 aatcaggagg cctgttgttt ttggggcgag gtggggtgtt tgataatcca aaaccacaaa
     7681 atggctgcgg tcgctgccaa tgcggctaag aggatggaca gcgttgttgt cggggtgttg
     7741 aaattttgtg cggcgaattt ggccaaagcc gagccactga agttgcccaa ttgtatgcag
     7801 gcattgaaca agcacaaaac catgtacaag gctgcgggtg ggtactccaa caaacggatt
     7861 tgcatcatca cattgaccaa tgaaaaggca atgctgatga gaaacacgcc caccaagccc
     7921 aaacgataat caaacaagaa cgacaaggca aagatggcag caaacatcgt gtacaaagta
     7981 ttgaagccaa tgtagcggtc gatttgatgg tcggcagccc aaccgcctgc gatttgccca
     8041 acgataaatc cgatgcccaa aataatcaat accaagatgc ggaggttgag gcttgggtga
     8101 gggtgcaaat taaaccaagc tatgctgtaa gtccaagccg cagaagtact gatgagaatg
     8161 cacaagccca ctgcaaacag cagccataag cggcgatttt ttaaggcatg caattgcatg
     8221 ctgatggttt gtttgatggc ggggactctg ctttgtggca ataagtcatg aatcaataag
     8281 aaaatgagca aatccaataa ggcaacaaat cgaaaagaaa tttgccagcc ataatccaaa
     8341 cctatccatg tgttgaatgg caccacaaat aaagtggaca aggaaatacc caccaaactc
     8401 aaacccaaat accgcccgcg tttattgggc ggggctattt cggctatcag taaggtggaa
     8461 aatgccagaa atgccgcatg cggaatgcca ctgaaccacc gcaaccaata caacatgtcg
     8521 gcgctggtgg cgaaagaagt cagtaaattg cctaaaaaac accacacgct gagcaaggtt
     8581 aaaatgcggg gtttagacca attgcgaaac gcaatggcca taatgggcgc cgcaatcaag
     8641 ccgccccaaa gataagcaaa aaccgcatgt aaaatttcac tggggctgct gtttaaatca
     8701 gcagctatgg cgggaaccaa aggaaaaatg gagaattttg cattgcccat ggcgaacgca
     8761 ccaaacatca acacccacaa gcaacgcatg gggctggcat tggtgtcatt ggcatcggca
     8821 gaggggggca tatacattcc aatcaggcgg tagggcagtg aaaacacatc acagacacat
     8881 tcagactctc aaacgtgtca ataatttccg tagggctcaa gtctttgaag cagcgaaatc
     8941 agcattttca aggcgacatc ttattccagt tttttgtttg ctgaaaagat tttctgcgag
     9001 gaaggatggc aatttgaaat agcgattgct tacacttgaa cagggtaaca tttatgttct
     9061 tagatttaaa agaataaatg agacagtttg tctaataccc ttgttgcaga aaggtgcaca
     9121 tgcttctgat actggcaatg gtgacaggcc aaaatgagct gtttcatgtg ccataacata
     9181 aagtgattag gcggttgcca gtttttgaat catggaagcg gcctgtaaac ctttacaggc
     9241 cgctttgagg ctttttctct cgcgcccagc gatgtttcaa ttttagatgg gcggggtttc
     9301 ggcttgtaac tgtttgcttt tggcgatgaa cgattgcaat aggctttgag attgctgtaa
     9361 ctgctccaac ttctgctcaa tcgaaaccaa ttcttgctca accaactcat tggcaatgcg
     9421 gcagagtttg aacgattggc tgtccaaatc gaaacaaggc agcagccagt ggatggcttt
     9481 gaggttcatt cctgcatcat tcaagatttt gattttgttg atgcattcga tgtcgtgcgc
     9541 actgtagctg cggtaattgt tgtcaccgcg cttgggcgca atcaaaccca attcctcgta
     9601 atagcgcaac atgcgtgggc tcacgtctgt gagcgccgat actttggcca aattcatgac
     9661 tttcctcttg actgtaacag cactgttata gtttaccatg actgacctga tagatgctgg
     9721 acagcggctt taccaattgt tttggcacga taccaacgga aagcctgtca ggataccttt
     9781 caatacccct caatggagcc atcatgacta ccgataaaca atccagctca tctttatggc
     9841 tcatggtctt ggctggcagc ctcatcttga tgattaccat gggcgtgcgc aattctttgg
     9901 gtttgtacat acagcccatc agtctatcca ccagcgtcaa tatcgcttcc atcagcttgg
     9961 ctttggcggt ggggcaattt ttatgggggg cgatacaacc catcgcaggg gccttggccg
    10021 accgttatgg ttcgcgcccc atcttgttaa taggcatggt gatattgatt ttgggtttgg
    10081 tcatagcacc gatgtggacc agctcgtttg gcttgatggt gagcttgggg gtgttgtctg
    10141 ccattggttc gggcataggc agcttttcag tcttgatggg tgcggtggcg caacacatca
    10201 cgccgcaaat gcgcggcaat gccacgggaa ttttgaacgc aggcggctcg tttggtcagg
    10261 tggtgttttc accattgata caggtgttga ttaaatcggt ggggtggatg cagtcattgt
    10321 ttgtggtggc ggcgattacc ttaagcacga taccgttgat ttttgccgcc accaagccca
    10381 aatcggcggc cagtactgtg gctcaagaca ataatggcat cggtttgaag caaacggtgt
    10441 tacaggcttt gcaaaaccgt gattatttgc tgttgcattt gggttttttc acttgcggct
    10501 ttcacatcgc tttcttggtg acccatttgc caggcgaggt ggcgatatgc ggcctgaacg
    10561 ccaaagtagc gggttggtcg ctggccatca taggcttggc caatgtggcg ggcagcttgc
    10621 tggctgggcg ttgggtatcg cgctaccgca gcaaatacat cttgttttgg atgtatgcaa
    10681 t
//
LOCUS       DAOMVK010000015        10318 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_1577_length_10318_cov_2.971938, whole genome shotgun sequence.
ACCESSION   DAOMVK010000015 DAOMVK010000000
VERSION     DAOMVK010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 10318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10318
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1577_length_10318_cov_2.971938"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(406..1218)
                     /gene="prmC"
                     /locus_tag="PK856_01200"
     CDS             complement(406..1218)
                     /gene="prmC"
                     /locus_tag="PK856_01200"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRL01018.1"
                     /translation="MSTIRDWLQHSPLPKLEARMLLQYVGDFSRAYLVAHDDEVLPDM
                     MVWQLNDMQQARLDGVPMAYLLGEREFYGRVFQVTPAVLIPRPETELLVETVMQYLPQ
                     GGRVWDVGTGSGIIAITTALERSDAQVHASDISAEALDIARNNTGRLGAQVAFHLGSW
                     YAVEPSQNELVDVLVSNPPYIHKDDGHLQQGDLRFEPQQALTDFDDGLSAYRALAADC
                     AQVVKPQGWLMVEHGFEQAADIQAIFQAAGLQQVHTLQDYAGLDRITLGQRA"
     gene            complement(1215..2645)
                     /gene="mgtE"
                     /locus_tag="PK856_01205"
     CDS             complement(1215..2645)
                     /gene="mgtE"
                     /locus_tag="PK856_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HRL01019.1"
                     /translation="MVDKEPDFQPSEERNEWDMERIHELAERLLPIYADIATGQTQEP
                     KTQRYLNELIGILHDLHPADMATVLESLPQQERVMVWKLSEPDEDGEVLLEVSDAVRE
                     TLIESMGQRELLAAIEDLDADELAELAPDLPKEVVHEALESLDAEERAQVVSALSYEE
                     DEVGALMDFEMVSIRADVTCEVVLRYLRRFESLPDHTDKIFVVDDDDVLQGVLPIRSL
                     LVSDPEELVENVMSTDVVRFRPDDDVEEAAAAFERYDLVTAPVVDNNKKLIGRITVDE
                     MLDVIREESEADMLNMAGLKEEEDLFAPIIDSFKNRWMWLVINLCTAFVASRVIGMFE
                     GSIEKIVALAALMPIVAGIGGNSGNQTITMIVRSLAMGQLNLSQSGRLLRKEFGVALL
                     NGIIWGSVMGVVSYLLYGSIGIGLVMVAAMTLNLVLAATMGVLIPVIMEKFGRDPALG
                     SSVLITAVTDSGGFMIFLGLATIFLL"
     gene            2817..3674
                     /locus_tag="PK856_01210"
     CDS             2817..3674
                     /locus_tag="PK856_01210"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRL01020.1"
                     /translation="MKLITLIASLFSVTLGLTACQPTQTELQAASGAKTIPQNTNNTS
                     NPVDTHQTHSIQVVLEWEHLQAETITAPEHGQLEQVLVQEKQQVIAGQMLAQFKADAT
                     SSVKRISGSSVNQAAKAQAYQKWQQDKKLQAQGFISEAAVAKSEAAYHIAAQAQTTVT
                     QTIVPAPPSKPVWIQSPFNGVIKPFTVSAGSKVKNGQQLFTIEPQDTKRLKIIVAQEN
                     NLPLQVGQRLHLHKKGFDLWLQISELPLNSDKQYTVAYAIWPNDVSKVDLGTVNAEIV
                     LNKNTTKPN"
     gene            3697..4215
                     /locus_tag="PK856_01215"
     CDS             3697..4215
                     /locus_tag="PK856_01215"
                     /inference="COORDINATES: protein motif:HMM:NF028042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HRL01021.1"
                     /translation="MQTLAISKQRGFTLLEAMVVVIIIGILAAIAYPSYLRYVRDARL
                     QNAKTELALAQNMLEKYYAQNRSFRKSGVSPAAYPTEILTNIQGNNTYFNIAFYSQDK
                     SGTPAMCNPANASGEQYCLYAAPISATNNGETRFVFADATGVIQECERGSANISNTGV
                     LTNKWDAICEAK"
     gene            4269..4706
                     /locus_tag="PK856_01220"
     CDS             4269..4706
                     /locus_tag="PK856_01220"
                     /inference="COORDINATES: protein motif:HMM:NF028042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HRL01022.1"
                     /translation="MSNKNQNSRYEASGFTLMEAMVVVIIIAILAAIALPSYHKFILN
                     ARLKEAKTTLIEDARFLERYYIQNARFTQNATTWPTLPFLNTDYFDISFKGAARGAAA
                     NTFRLQAIAKNTAEEPRYLTIDQNHIIQECIKVGSSTRCHSPE"
     gene            4799..5077
                     /locus_tag="PK856_01225"
     CDS             4799..5077
                     /locus_tag="PK856_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HRL01023.1"
                     /translation="MSDENTPHETVMEFPCEFPLKIMGTHHDEFAQTILAVVQEHAPD
                     TQEHHVSTRLSSKGNYIGATVVITATSQEQLDNIYRSLTSHPMVKVVY"
     gene            5077..5697
                     /gene="lipB"
                     /locus_tag="PK856_01230"
     CDS             5077..5697
                     /gene="lipB"
                     /locus_tag="PK856_01230"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HRL01024.1"
                     /translation="MLIKHLGRVEYEPTYQAMVSFTDTRTDETVDELWIVEHPPVFTQ
                     GMAGKPEHLLQHSQIPVVQVDRGGQITYHGPGQLVVYTMIDFKRRHITVRDLVSRLEN
                     SIIATLAHFGIEAHNDPKRPGVYVDERKIASLGLRIKRGSVYHGLALNIDMDLTPFHQ
                     INPCGYAGLEMVQMAELVSPAPELNQVADILADQLQQQLTVEEAHT"
     gene            5694..6647
                     /gene="lipA"
                     /locus_tag="PK856_01235"
     CDS             5694..6647
                     /gene="lipA"
                     /locus_tag="PK856_01235"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HRL01025.1"
                     /translation="MSQDNAQGVKHKGAAKTARIPIKVVPLETKLKKPEWIRAKLPSG
                     GQFMEIKRILREQKLHTVCEEASCPNIGECFSKGTATFMIMGDICPRRCPFCDVGHGR
                     PNPLDPEEPKHLAETVAALKLKYVVITSVDRDDLRDGGAGHFADCIKAIRELSPSTQI
                     EVLVPDFRGRLDIALEILAETAPDVMNHNLETAPRLYKQARPGADYKHSLELLKRYKA
                     MRPDVATKSGIMVGLGEEDEEVREVMRDMREHDIDMITIGQYLQPTDGHLPVLRYVSP
                     QMFKTFETEALDMGFKHAAVGAMVRSSYHADEQAKDAGFAE"
     gene            6727..8229
                     /gene="ppx"
                     /locus_tag="PK856_01240"
     CDS             6727..8229
                     /gene="ppx"
                     /locus_tag="PK856_01240"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HRL01026.1"
                     /translation="MPRFPLLATVDLGSNSFRLQICAVEEGQLRIVDSIKEMVRFAAG
                     LDENKNITLDAQQRALKCLSRFGQRLAGFKPEQVRVVATNTFRVARNIASFLPQAEEA
                     LGFPVEIIAGREEARLIYLGVKHTQPPTNDNMLVVDIGGGSTEFVIGNALQPDIMESL
                     PLGCVTYSMRFFSDGKITSERFERAVSSARIEIQRISPELKLHGWDFAIGSSGSARSI
                     RDVIEAYGLSKHHLDAASMRVLAKKVIQAGSVKKAKLLNLKPDRTEVFLGGLAVMIAV
                     FEELSLESMSVTDAALRDGVFFDMLGRDLDHDLRDDTVKQFQHRYHIDITQAARVNQV
                     AQQCFAGLSQNLPRNQLESWQKYLAWSAQLHELGMSIAHSGYHKHSAYILDNADMFGF
                     SRQEQHLLAVLVLTHRGDTKKLLEVVIDNQWWPAVLSLRLAALFCRGRQDIQFPSDSS
                     LYYDFTEKACTLQLSAKWLQDNPLIAGSLEQEAQQWQYINRKLIIEQQAA"
     gene            8241..9224
                     /gene="corA"
                     /locus_tag="PK856_01245"
     CDS             8241..9224
                     /gene="corA"
                     /locus_tag="PK856_01245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HRL01027.1"
                     /translation="MSETGLQVQLHQAALTGDAEVVTDLSCTQTVPKPASPHWLNISG
                     KASLADLQLLLGEYRLHALVLEDMASKNQRAKIEDYGDFVFLVLRGVLFQDSQLKTQL
                     LYLIIGKDFLISYQPKSLLMRPVMKQRIAQKPLWYQQSNLEYLMYLYIDCWVDTLMAS
                     VETFSSKVDKLDGTLLQVKDTDLLPRLHRMKHDAMRLRRSVLPLRDVLTILMRSDFEV
                     LSDRYHLYMRDTFDHCMQLMENLDFSRDALLTMMEVTLGAQSNRLNRQMRLLTAVSIT
                     FMPLTLITGIYGMNFDNMPELHWEYGYFYVLATITIIAISSIAFLIHRKWL"
     gene            9257..9766
                     /locus_tag="PK856_01250"
     CDS             9257..9766
                     /locus_tag="PK856_01250"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRL01028.1"
                     /translation="MPKLDHPTIVEAQSELTMSILMTPDMANFSGNVHGGAILKLLDQ
                     VAYACASRFSGSYVVTLSVDKVTFKEPIYVGELVNFLASVNHVGRTSMEIGIRVEAQD
                     IRNRTVRHTNSCYFTMVAVDENGKPVEVPRLNLDNNLKLCRFEAAEHRKQMRLETVQH
                     SSCSIFKDQ"
     gene            9766..>10318
                     /locus_tag="PK856_01255"
     CDS             9766..>10318
                     /locus_tag="PK856_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase subunit alpha"
                     /protein_id="HRL01029.1"
                     /translation="MFTGIVQGLAEITAIEDRGEFRRHTITLPPELAQDMTIGASVAH
                     NGCCLTITEFTGQQVSFDLIEETLRCTNLGDLKVGDVVNIERAARFGDEIGGHVMSGH
                     IATTTHIVAIEKTDFNTTVWFVLPDKLKPYVLTKGFVGLDGCSLTIGDVLSDRFNVHL
                     IPETLERTLFGSRQVGDAINIEID"
BASE COUNT         2880 a         2660 c         2268 g         2510 t
ORIGIN      
        1 ccccatcatc gaagctgaac gccaattctt tggccagcac aaaaggggca aaacacaaaa
       61 ccgccaaggg caacaacttt ttcaacatac gccatcctaa tctgaagtgg aagctgggtt
      121 tatatcactg gcatgcgcag caatggcaag ctttgccatg aatttacatg cgatgcatgg
      181 caataatcgg ctgggctggc agatggtatg gcaccaattt ttgtgatgtg tcctcaaaat
      241 acccgacaca cccattcaaa cgaatacctg ttaacaccaa gccacatggc atccttattc
      301 attctgcaat gctttgagtt ttatacatgc ttacaggccg cattaagcag cctgtaattg
      361 tgatgctgca catacgagtg gcaccaaatc acacgcattg acagcctatg cccgttgtcc
      421 caaggtaatg cggtctaagc ccgcatagtc ttgcaaggta tgcacttgct gcaatcctgc
      481 agcttggaaa atggcttgta tgtctgccgc ttgttcaaaa ccatgctcaa ccatcagcca
      541 accttgtggt ttcacaacct gagcgcaatc agccgctaaa gcgcggtatg cgcttaaacc
      601 atcgtcaaaa tcggtgagcg cttgctgtgg ctcaaagcgc aaatcgcctt gttgcaaatg
      661 gccatcgtct ttgtggatgt aaggcgggtt cgacaccaac acatcaacca actcgttttg
      721 cgatggctcc accgcatacc aagaacccaa atgaaacgcc acttgcgcgc ccaagcggcc
      781 tgtattgtta cgggcaatgt ccaaagcctc ggcgctgatg tctgaggcat gcacttgggc
      841 atcgctgcgt tctagcgctg tggtgatggc gatgatgcca ctgcctgtgc ccacatccca
      901 cacccgcccg ccttgcggca ggtattgcat cacggtttcc accagcaatt cagtttcagg
      961 ccgcggtatc aacaccgcag gcgtgacttg aaacacgcgc ccataaaatt cgcgctctcc
     1021 caacaaatat gccatgggca cgccatctaa gcgcgcttgt tgcatgtcgt tgagctgcca
     1081 caccatcatg tcaggcaaca cttcatcatc gtgcgccacc aaataggcgc gagaaaaatc
     1141 accgacatat tgcaataaca tgcgggcttc aagtttggga agtgggctgt gctgcagcca
     1201 atcgcgtatc gtactcataa taggaatatt gttgccaagc ctaagaaaat catgaatccg
     1261 cccgaatcag tgacggcggt aatcaaaacc gaactgccca aagcgggatc gcgaccaaac
     1321 ttttccatga tgacgggaat caacacgccc atggtggcgg ccaacaccaa attcagcgtc
     1381 atcgcggcca ccatcaccaa accgatgcct atgctgccgt aaagcaaata cgacaccacc
     1441 cccatgaccg agccccagat gatgccattg agcaaggcca cgccaaactc tttgcgcagc
     1501 aagcggcctg attggctcaa attcaattgt cccatcgcca aagagcgcac aatcatggtg
     1561 atggtttggt tgcccgagtt accgcctatg cctgccacaa tcggcattaa tgctgccaaa
     1621 gcaacaattt tctcaatcga gccttcaaac atgccaatga cgcgactggc cacaaatgcg
     1681 gtacacaaat taatcaccaa ccacatccaa cggtttttga aggaatcaat aatcggcgca
     1741 aacaaatcct cttcttcttt caaacccgcc atattcagca tgtcggcttc ggattcctct
     1801 cgaatcacgt ccaacatttc gtcgacggtg atgcggccta tcagtttttt gttgttgtcg
     1861 accacaggcg cggtcaccaa gtcgtagcgc tcaaacgcgg cagccgcttc ttccacatca
     1921 tcatcaggcc gaaaacgcac cacatcggtg gacatcacgt tttcaaccaa ttcttccggg
     1981 tcggacacca gcaagctgcg tatcggcaac acaccttgca acacatcgtc gtcatccacc
     2041 acaaaaatct tatctgtatg gtcaggcagg ctttcaaaac ggcgtaaata gcgaagcacc
     2101 acctcacaag tcacatcagc acgtatgctg accatttcaa agtccatcaa cgcaccgact
     2161 tcgtcttctt catacgacaa agccgacacc acttgagcac gctcttcagc atctaaagac
     2221 tctaatgctt catgcaccac ttctttgggc aagtcaggcg ccagctctgc caactcatct
     2281 gcatccaaat cctcaatcgc agccagcagc tcacgctgcc ccatgctctc aatcagtgtt
     2341 tcgcgcaccg catccgacac ttccaacaat acttcgccgt cttcatctgg ctcagacagc
     2401 ttccacacca tcacccgttc ttgctgtggc aacgactcca acaccgttgc catatccgca
     2461 ggatgcaaat cgtgtaaaat gccgattaat tcattcagat agcgctgggt tttgggctct
     2521 tgggtttggc cagtggcaat gtcagcataa atgggcaata agcgctctgc caattcatgg
     2581 atgcgctcca tatcccattc attgcgctct tcagatggct ggaaatcagg ctctttatcg
     2641 accataaaac aactctttca acaacgggaa catcggatta ggggaagtgc gggctattgt
     2701 acctgtttta agaccaagac ttaagccaag actgcaagtc agacaatact tagacttgct
     2761 gaacatgaaa atcgataacg atgtcaaaat gatgacaata tgccatgatg ccaaaaatga
     2821 aattaattac tctaatcgcc agcttgtttt cagttacttt aggcttaaca gcatgtcagc
     2881 ctacacaaac ggagttacag gccgcatctg gcgccaaaac aatacctcaa aatacaaaca
     2941 acacaagcaa tcccgttgat acccatcaaa cgcactccat acaagtggta ttagaatggg
     3001 aacatttaca agccgaaacc attaccgcac cagagcatgg tcagttagag caagtgctgg
     3061 ttcaagaaaa gcaacaagtg attgcaggac aaatgttggc acagtttaaa gccgatgcca
     3121 cgtcatccgt caaacgcatc agcggcagtt cagtcaatca ggctgctaag gcacaagcct
     3181 atcaaaagtg gcaacaagat aaaaaattac aagctcaggg gtttatctca gaagccgcag
     3241 tggccaaaag cgaagccgct taccacattg cggcacaagc ccaaaccaca gtgacacaaa
     3301 ctattgtacc tgctcctcca agcaaaccgg tttggataca atcaccgttt aatggtgtaa
     3361 tcaaaccatt tacagtaagt gctggtagta aggtaaaaaa cggtcaacaa ttgtttacaa
     3421 ttgagccaca agacacaaaa cgcctcaaaa tcattgtagc tcaagagaac aatctgcctt
     3481 tacaagttgg ccaacggcta catctacata aaaaagggtt tgatttgtgg ttacaaatca
     3541 gtgaattgcc cttaaacagt gacaagcaat acactgtggc ttatgccatt tggcccaatg
     3601 atgtttcaaa agtagattta ggcacagtaa atgcagagat tgtactaaat aagaatacga
     3661 caaaaccaaa ttaatcaaat ctaatggatt ttaagcatgc aaacattggc aatcagcaaa
     3721 caacggggat ttactttatt ggaagccatg gtggtggtca tcatcatcgg gattttggcg
     3781 gcgattgcct atccaagcta tcttcgatat gtacgtgatg cccgtttgca aaatgccaaa
     3841 acagaattgg cattggctca aaatatgtta gaaaaatact atgctcaaaa ccgcagtttt
     3901 agaaaatctg gcgttagccc tgcggcttac cccactgaaa tattaaccaa tattcaagga
     3961 aataatactt atttcaacat cgccttttac agtcaggaca aatccggcac tccagccatg
     4021 tgtaatcctg ccaatgcaag tggtgaacaa tactgtttgt acgcagcgcc aatcagtgcc
     4081 accaacaatg gagaaactcg ctttgtcttt gctgatgcta caggagtcat acaagaatgt
     4141 gaacgaggct ctgcaaacat cagtaatact ggagtcttaa ccaacaaatg ggatgcaata
     4201 tgcgaggcca aatagtttag tagagtcatc ttctttgact ctcaaactga gaggcttagg
     4261 agatgaacat gtctaacaaa aatcagaatt ctcgttatga ggcttcagga tttaccttga
     4321 tggaagcaat ggttgtagtc attatcattg ctattttggc ggccattgca cttcctagct
     4381 accataaatt tatactcaac gcccgcttga aagaagccaa gaccacgctt attgaagatg
     4441 ctcggtttct tgagcgttat tacatccaaa atgcccgttt cacccaaaat gccactactt
     4501 ggccaacctt accctttctt aataccgatt attttgacat cagttttaaa ggggccgcac
     4561 gtggtgccgc agccaataca tttcgactgc aagcaatagc aaaaaataca gcagaagagc
     4621 cacgatacct caccatagac caaaaccata tcatccaaga atgcatcaaa gtgggtagca
     4681 gcacccgttg ccatagccca gaataatggc ttttatagac tttccatacg gtgcttgggt
     4741 agtgacgcaa actagcgtaa aatgatgcct ttagatacct ctaattaaag gcgttatcat
     4801 gtctgatgag aacacacccc acgaaaccgt gatggaattt ccttgtgagt ttcctctgaa
     4861 aatcatgggt acccatcacg atgaatttgc gcaaaccatt ttggctgtgg ttcaagagca
     4921 tgccccagac acccaagaac accatgtcag cactcgctta tccagtaaag gcaattacat
     4981 tggtgcaaca gtggtcatca cagccaccag ccaagagcaa ctggacaata tctaccgcag
     5041 cctcacctca caccccatgg tgaaggtggt ttactgatgt tgataaaaca cctaggacgc
     5101 gtggaatatg agcccactta ccaagccatg gtgtccttca ccgacacgcg cacagatgaa
     5161 acggttgatg agttgtggat agttgagcac cccccagtat tcacacaagg tatggcaggc
     5221 aaacccgaac acttgttaca acacagccaa atccctgttg tccaagtcga tcgtggcggc
     5281 caaatcactt accatggccc tggccaattg gtggtttata ccatgataga ctttaagcgc
     5341 cgccatatta ccgtgcggga tttggtcagt cgtttggaaa acagtatcat tgccactttg
     5401 gctcattttg gtattgaagc ccacaacgat cctaaacgcc ctggtgtgta tgtggatgaa
     5461 cgcaagattg cctctttagg tctcagaatc aagcgaggct cggtttacca tggattggct
     5521 ttaaatatcg acatggactt aacccctttt catcaaatca atccctgtgg ctatgctggc
     5581 ttagaaatgg tacagatggc cgaattggtg tcacccgcac ctgaactcaa tcaagtcgcc
     5641 gacatactcg ccgaccaatt acaacaacaa ttaaccgtgg aagaagcaca cacatgagcc
     5701 aagacaatgc ccaaggcgtg aaacacaaag gcgcagcaaa aaccgcacgc attcccatta
     5761 aagtggtgcc tttggaaacc aagttaaaaa aaccagaatg gattcgtgcc aagctgccta
     5821 gcggtggcca attcatggaa atcaagcgca tcttgcgcga acaaaaattg cataccgtgt
     5881 gcgaagaagc ttcttgcccg aacataggtg aatgtttcag caaaggcaca gccaccttca
     5941 tgattatggg tgacatatgt ccccgccgct gcccattctg tgatgtgggt cacggtcgtc
     6001 ctaacccgct agatcccgaa gaacccaaac atttggcaga aactgtggcc gcattgaaat
     6061 tgaaatatgt ggtgattacc tcggttgacc gcgatgactt gcgtgacggt ggtgcgggcc
     6121 actttgccga ttgcatcaaa gccatacgcg aattgagccc cagcacccaa attgaagtgt
     6181 tggtaccaga cttccgtggc cgcttggaca ttgctttgga aattttggcc gaaaccgccc
     6241 ctgatgtgat gaaccacaac ttggaaaccg caccacgcct ctacaaacaa gcacgaccag
     6301 gtgctgacta caagcactca ttggaattgc tcaaacgcta caaagccatg cgtcctgatg
     6361 tggccaccaa atcaggcatc atggtaggtt tgggtgaaga agatgaggaa gtgcgtgaag
     6421 tgatgcgtga catgcgtgaa cacgacatcg acatgattac catcgggcaa tatttacaac
     6481 cgaccgatgg ccatttaccc gtgttgcgct atgtgtcgcc acaaatgttc aaaacctttg
     6541 aaacagaagc gttagacatg ggcttcaaac acgcggctgt tggtgccatg gtgcgctcta
     6601 gctaccatgc cgatgaacaa gccaaagatg ctggctttgc tgaataaccc gtatcactga
     6661 gctaaggcgg cctgaactga caggccgctt taaagactca tccaatattt caataggatt
     6721 accggcatgc ctcgctttcc acttttggcc accgtcgact tgggttctaa cagttttcgc
     6781 ttacaaattt gtgcggttga agaaggtcaa ttgcgcatcg tcgactccat caaagaaatg
     6841 gttcgctttg ccgcaggcct ggatgaaaat aaaaacatca cattagacgc acaacaacgg
     6901 gcattaaaat gcttgtcacg ttttggtcaa cgccttgctg gctttaaacc agaacaagtg
     6961 cgcgtggttg ccaccaatac tttccgcgtt gcacgaaaca tcgccagctt tttgcctcaa
     7021 gccgaagaag ccttgggttt cccagtggaa atcattgcag gtcgtgaaga agcacgcttg
     7081 atttatttgg gtgtgaaaca cactcagccc cctaccaatg acaatatgtt ggtggtggac
     7141 attggtggcg gctcaaccga atttgtgatt ggcaatgcct tacagccaga catcatggaa
     7201 agcctgcctt tgggctgtgt cacctacagc atgcgttttt tcagcgacgg caaaatcacc
     7261 tcagaacgct tcgaacgcgc tgtttcaagt gcccgcatcg aaatccaacg catcagtccc
     7321 gagctgaagt tacatggctg ggattttgcc ataggcagct caggctctgc ccgctccatt
     7381 cgcgatgtga ttgaagccta tggtttgagc aaacaccact tggatgctgc ttccatgcgg
     7441 gtattggcca aaaaagtcat acaggccggt tcggttaaaa aagccaagct gctcaattta
     7501 aaacccgacc gtaccgaggt tttcctcggc ggcttggccg tcatgattgc cgtatttgaa
     7561 gagctgagcc ttgaatccat gtcagtcacc gacgccgctc ttcgtgacgg ggtctttttt
     7621 gacatgttgg gccgtgattt agaccatgat ttgcgcgacg acaccgtgaa gcaatttcaa
     7681 caccgttacc acattgacat cacccaagcg gcccgagtga atcaagttgc ccaacagtgt
     7741 tttgctggct tgtcgcaaaa cttgccacgc aatcaattgg aatcttggca aaaatatttg
     7801 gcttggtctg cgcaactaca tgaattgggc atgagcattg cccattctgg ttaccacaaa
     7861 cattcagcct acattttgga caatgccgat atgtttggct tttcacgcca agagcaacat
     7921 ttgctggctg ttttggtatt gacccaccgt ggcgatacca aaaaattgtt ggaagtggtc
     7981 atcgataacc aatggtggcc tgcagtattg tctttgcgct tggcagcttt gttctgtcgt
     8041 ggccgccaag acattcaatt tccaagcgac tccagtctgt attatgactt tacggaaaaa
     8101 gcctgcacct tgcagctcag tgctaaatgg ctacaagaca atccattgat tgcaggcagc
     8161 ctagagcaag aggcgcagca atggcaatac atcaaccgca aattgatcat tgaacagcaa
     8221 gcagcctaat ggaacccaaa atgtctgaaa cgggcttgca agtacaatta caccaagccg
     8281 cgctcacggg tgatgctgag gtggtgaccg atttgtcttg cacccaaacc gttcccaaac
     8341 cagcttcacc ccattggctc aacatcagtg gcaaagcctc tttggccgat ttgcaattat
     8401 tactgggaga gtatcggctg catgctttgg ttttggaaga catggccagt aagaaccagc
     8461 gcgccaaaat cgaagactat ggcgattttg tgtttttggt cttgcgtggc gtgttgtttc
     8521 aagacagcca actcaaaacc caattattgt atttaatcat tggtaaggat tttctgattt
     8581 cctatcagcc caaatcccta ttgatgcggc ctgtaatgaa gcaacgcatt gctcaaaagc
     8641 ccttgtggta tcagcaaagc aatttagaat acctgatgta tttgtacatt gactgctggg
     8701 tagacacctt aatggcgagt gtggaaacct tcagtagcaa agttgacaag ctcgatggca
     8761 ccttattgca agtcaaagac accgacttac tgccccgttt gcaccgcatg aagcacgatg
     8821 ccatgcgttt gcgccgctcg gtattgccct tgcgcgacgt attgaccata ttgatgcgtt
     8881 ccgattttga agtgttgtct gaccgttacc atttgtacat gcgcgacact ttcgaccact
     8941 gtatgcaatt gatggagaat ttggatttca gtcgcgatgc cttgctcacg atgatggaag
     9001 tcacactggg cgcacaatcg aatcggctga accgccaaat gcgtctgctg acagcagtca
     9061 gcattacctt tatgccgctg accttgatta caggcattta cggcatgaat tttgacaaca
     9121 tgccagaatt acactgggaa tacggttatt tctatgtttt ggcaaccatc actatcatag
     9181 ccataagttc aattgcattc ctgattcacc gaaaatggct ataatcacaa tcattaagaa
     9241 atgaattgag tagaacatgc ccaaattaga ccaccctacc attgtagaag cccaatcaga
     9301 attaacgatg tccatcttaa tgacacctga catggccaat ttctctggca atgtgcacgg
     9361 tggcgcgatt ttaaaattgc tagaccaagt ggcttatgct tgcgccagcc gcttttctgg
     9421 cagctatgtg gtgaccttat cggtagacaa agtcaccttc aaagagccga tttacgtggg
     9481 cgaattggtc aacttcttgg ccagtgtcaa ccatgtgggc cgcacttcca tggaaattgg
     9541 cattcgtgtt gaagcacaag acattcgcaa ccgcaccgtg cgccatacca acagctgtta
     9601 tttcaccatg gtggcagtag acgagaacgg caaacctgtc gaagtgccgc gtttgaattt
     9661 ggacaacaac ttgaagctct gccgctttga agccgcagaa caccgcaagc aaatgcgttt
     9721 agaaaccgtg caacacagtt cttgcagcat ttttaaggac caataatgtt tacaggcatc
     9781 gttcaaggtt tggctgaaat cactgccatt gaagaccgtg gtgagtttcg ccgccacacc
     9841 atcaccctgc ctcctgaatt ggcacaagac atgaccatag gcgcctcagt ggcccacaat
     9901 ggctgctgcc tcaccattac cgagtttaca ggtcaacagg tctcttttga cttgatagaa
     9961 gaaaccctgc gctgcaccaa tttaggcgac ctgaaagtcg gcgatgtggt taacattgaa
    10021 cgtgccgcac gctttggtga tgaaattggt ggccatgtga tgagtggcca cattgccacc
    10081 accacccaca tcgtggccat tgaaaaaacc gatttcaaca ccactgtgtg gtttgtactg
    10141 ccagacaagc tcaaacccta tgtgttaacc aaaggctttg tcggcttaga tggctgctca
    10201 ctaaccatag gtgatgtgtt atccgaccgt ttcaatgtgc atttaatccc tgaaacccta
    10261 gaacgcacct tgtttggcag ccgccaagtc ggcgatgcca tcaatattga aatagatg
//
LOCUS       DAOMVK010000016        10293 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_1586_length_10293_cov_3.468744, whole genome shotgun sequence.
ACCESSION   DAOMVK010000016 DAOMVK010000000
VERSION     DAOMVK010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 10293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10293
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1586_length_10293_cov_3.468744"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /gene="minD"
                     /locus_tag="PK856_01260"
     CDS             <1..611
                     /gene="minD"
                     /locus_tag="PK856_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HRL01030.1"
                     /translation="LNQALIKDKHCDNLYILPASQTRDKDALNRDGVGRVLKELTENM
                     GFEYVVCDSPAGIEQGALMALYYADEAIVTTNPEVSSVRDSDRILGILSSRSRRAEMS
                     EAPIKEHLLITRYAPARVVTGDMLSVDDITDILRIPLLGVIPESGAVLQASNSGSPAI
                     HLANTDVSDAYKDVVARLMGESRPMRFVTDEKKGLLKRLFGG"
     gene            615..887
                     /gene="minE"
                     /locus_tag="PK856_01265"
     CDS             615..887
                     /gene="minE"
                     /locus_tag="PK856_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HRL01031.1"
                     /translation="MSLLDLLLGRKKNTAATAKDRLQIIIAQERVQSQAPDYLPMLKK
                     ELMEVLARYVNISPDDIRISQEQHDGLEMLELNITLPEPKKEKAST"
     gene            884..1804
                     /locus_tag="PK856_01270"
     CDS             884..1804
                     /locus_tag="PK856_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRL01032.1"
                     /translation="MTLTELRYIVAVAQEHHFGRAAQRCFVSQPTLSIAIKKLEEELG
                     VALFDRSSNEIRPTDAGLRILDQAQRVLEEADMIKQLAKEEQNELEGSFKLGLIFTVA
                     PYLLPKLIVSLRDLAPKMPLMLEENHTAVLTDMLKKGELDAIVVADPYQEAGVVTIPL
                     YDEPFFVITPKGHPFEEMDEITTQELADEQVLLLTEGNCMRDQVLDSCVELASRQRIK
                     GLTNTLQGSSINTIRHMVASGLGISVLPATSLTENDHMLFSIIPFKAPAPERRVLLAA
                     RRHFVRQKALDTIQTAIFHSQLAGVRFVNE"
     gene            1860..1936
                     /locus_tag="PK856_01275"
     tRNA            1860..1936
                     /locus_tag="PK856_01275"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1894..1896,aa:Met,seq:cat)
     gene            2054..2314
                     /locus_tag="PK856_01280"
     CDS             2054..2314
                     /locus_tag="PK856_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01033.1"
                     /translation="MNTSDNNPFALWQQFWQNANPQMAPFLPPMSAEEVQKKITELQS
                     VELWLSFNLQMVQSQLAVLEQQKLFFNSLNQMSENLSNTKPD"
     gene            2357..5815
                     /gene="dnaE"
                     /locus_tag="PK856_01285"
     CDS             2357..5815
                     /gene="dnaE"
                     /locus_tag="PK856_01285"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRL01034.1"
                     /translation="MTSPRYIPLRMHTEFSIEDGMIRIKDAVKHASGLGFAALGISDL
                     MNLFGMVKFYKACRGAGIKPIIGVDVWVQDDQTDDGDNKPYRLLLIARNQPGFKRLCE
                     LITQAYVDDDRDKMDFRLRYSWLESGDNSGLICLSGAHLGSVGQHLLSGRPEQAKQAA
                     SALSELFPNRFYLELQRLPEKPEWENAVVGSLNLAQELDLPIVATHPAQFLNKDDFQA
                     HEARVCIASGYVLADTRRPKNFYPSQYFAPADEFEARFADVPEALQNSVEIAKRCNVE
                     IVLGENFLPQFPTPDGMTLDDYLSHLSNEGLAERMAVLYPDETERAQRYPEYQQRLDY
                     ELNTIVQMGFPGYFLIVQDFINWAKQNGCPVGPGRGSGAGSLVAYALKITDLDPLKYA
                     LLFERFLNPERVSMPDFDIDFCQANRGRVIHYVRDHYGQDAVSQIVTFGTLSSKAVIR
                     DVGRVLDLPFSMCDRLSKLIPLEQNKPLSLKKAMETEPQIAEIIEAEEADELLVLAQK
                     LEDLTRGTGMHAGGVLIAPGKITDFCPVYQAQGEDTSPVSMFDKDDVEQAGLVKFDFL
                     GLRNLTIIEMAQNFIKDVTGDIVDVNHIPLDDADTYKHIFAEGNTTAVFQFESAGMRR
                     MLMEAEPSKFEEIIAFVALYRPGPMDLIPDFIRRMHGEKFEYLHELLSNVLEPTYGIM
                     VYQEQVMQAAQVCGGYTLGGADLLRRAMGKKKVEEMVKHRGIFVEGAAKNGITEKNAN
                     EIFDYMEKFAGYGFNKSHAAAYALVSYQTAWLKYHYPAEFMAATMSSELDNTDQLKIF
                     YDDATSRINGIRFLPPSVNASFYRFQPEAGKKIRYALGAIKGTGEAAVNAIVEAREKS
                     GAFTSLFDFCERVGKQHLNKRTVEALIRGGAFDEIEPNRAMLLGNVGLAMEYADQKAA
                     NANQGGLFDFIDDASAMPEVEMEPVAAWSESDKLAEEKQALGFYYSGHPFTPYRATIR
                     GFAKKNLADIKPADHKQWVGGFVTGVRTIMGRRGKMAIVIVEDETQKLEVVVAGSSYE
                     QHQHLLKTDQVLLFECKVARDEYNGGGGVRINSDGIYDFDGARSAFARSLCLTMTPNT
                     DVVKLSALLGNSRGQGYVGLKVSYEHPEVSAELKPKSPWQIGIDSALLKQLSELLGQD
                     AVEVVW"
     gene            6122..6664
                     /locus_tag="PK856_01290"
     CDS             6122..6664
                     /locus_tag="PK856_01290"
                     /inference="COORDINATES: protein motif:HMM:NF025639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRL01035.1"
                     /translation="MKQGMFVNVWQTAGLKHDEVRNSKVLQWFLNPNGNHGQSDALLK
                     QFITLLPIEFQNLDTQSSRVIAESCPLGEGTERVDIEVDAQHFLLFIEIKINAYEGQE
                     QLNRYLNIAQRKAHNRPWMVVYLTNQPQLPVMHRNKQNLIAISWQQVAKAFLQYSKQA
                     ETHNRSAWLVQQFAEHIQAF"
     gene            6680..7348
                     /locus_tag="PK856_01295"
     CDS             6680..7348
                     /locus_tag="PK856_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01036.1"
                     /translation="MSNKQAGKLIIENIQMLDEAVKLLSGDLSQQIYEGIEKVLQDKL
                     SEFSEEWSRSNELLSDFSYPFAPKKWRLIDSEQFRHKDYFARYCLTWESTHSEQETNQ
                     YLLTAFFKNSYDVIIFKFFPWFGCYARCTNGLWKKFATAQNEKYPEIESLGFKYSAKE
                     GEWYLPIDSLSQQLVADCYVEDNLADAMGPITEALEKLYQAHPYFDKIVEAAKAEFGV
                     NVPD"
     gene            7481..7933
                     /locus_tag="PK856_01300"
     CDS             7481..7933
                     /locus_tag="PK856_01300"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRL01037.1"
                     /translation="MKTIRSAIEIARPQEVVFDLTQNYAKRLEWDPYLAEAYLMKDAA
                     EPAVGVDAYCKNHNGSVMVSRYISFNRPAVAAVNMVKGPKILKRFSGAWNVHKMDDQH
                     SELIFTYHFDLRGGIAGEMITPIVRWHFQREMNKRLSAFKAYLEAESA"
     gene            7974..9104
                     /locus_tag="PK856_01305"
     CDS             7974..9104
                     /locus_tag="PK856_01305"
                     /inference="COORDINATES: protein motif:HMM:NF016973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF695 domain-containing protein"
                     /protein_id="HRL01038.1"
                     /translation="MMGFLQKLMGKHQAIVLDKVVHDKDTFWAWFQTQAAAFHHAVIT
                     QEKIDTDFFDPLSASLDRMHEGIYFLAGEAEAGVAELILTADGVYRNFAFIEDLVAAA
                     PKLPAWRFVALKPACAIDGFGINMNGLVYSDDTLHFYVREDAKCPQVVDVVMVHSDAT
                     ATEEQLTSGVYVYLDNLLGEWVAVTQIDQMQIINQQQLPANQTLQPMAELAPYLHSRH
                     EAWQKAYGGVYYNSDQDEFASFEGEADNGQLIVGIIDSTLLAWPHKPSHPWMLTLRIA
                     YDGSGLNGMPSKQIYQRMEEFEDALMAELPEHEGYLNVGRETGDDIRYIYFACKQFVQ
                     ATRVLERLMPQFSQEMDISYDVFQDKYWQSLQRFEHLSSAED"
     gene            complement(9179..9670)
                     /locus_tag="PK856_01310"
     CDS             complement(9179..9670)
                     /locus_tag="PK856_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HRL01039.1"
                     /translation="MPIRPADASDLPAIVAIYNSTVAGRQVTADLQPVSVDERLNWFD
                     AHQQPHRPMMVLCDEQDCVLAWATFSDYYARAAYDISAEISIYVDESQRGKRLGQTLL
                     DYMLALAPSLGIRNVIAVIFAHNQPSVRMFEKNGFSLWGTLPNVCELDDKEVSVVLLG
                     KRL"
     gene            9846..10265
                     /locus_tag="PK856_01315"
     CDS             9846..10265
                     /locus_tag="PK856_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009118038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HRL01040.1"
                     /translation="MKHVLLLSILCSAGLTMAQDISGKWRTIDDETKKPKAVVQISKN
                     GDSYSGRIISLEAGVENKCAGCSGAQKGGPLVGLTVVRGLKEDGKAYSGGSIFDPKSG
                     KTYKANAELANGGNSLKVRGYVGVSALGRTQTWQRVQ"
BASE COUNT         2794 a         2177 c         2661 g         2661 t
ORIGIN      
        1 ctttgaacca agccttaatc aaagacaagc attgcgacaa tttgtacatt ttgccggctt
       61 cacaaactcg tgacaaagat gcgttaaacc gtgatggtgt tggacgggtg ttgaaagagt
      121 tgactgaaaa catggggttt gaatacgtgg tgtgtgactc acccgcaggg attgaacaag
      181 gcgcactgat ggctttgtat tatgcggatg aagccattgt cactaccaat ccagaagttt
      241 caagtgtgcg cgattctgac cgtattttgg gcattttgtc tagccgaagc cgccgtgctg
      301 aaatgagtga agcgcctatc aaagagcact tattgattac ccgctatgct ccagcacgcg
      361 tggtgacggg tgacatgttg tcggttgatg acattaccga tattttgcgc attcctttgt
      421 tgggtgtgat tcccgaatcg ggtgcggtgt tgcaagcgtc aaactcaggt tcgccggcca
      481 ttcatttggc caataccgat gtttcggatg catataaaga tgtggtcgct cgtttgatgg
      541 gggaaagccg tccgatgcgt tttgttactg atgagaaaaa gggtttatta aaacgcttgt
      601 tcggaggata aggtatgtca ttactggatt tgttgctggg tcgaaagaag aacaccgcag
      661 ccacagcaaa agaccgtttg caaatcatca ttgctcaaga gcgtgttcaa tcacaggcac
      721 cagattattt gcctatgctc aaaaaggaat tgatggaagt attggcacgg tatgtcaata
      781 tttcacctga tgatattcgt atctcacaag agcaacacga tggcttggaa atgttggaat
      841 tgaacattac tttgccagaa ccgaagaaag agaaagcctc tacatgaccc taaccgagtt
      901 gcgctatatt gttgctgtgg cacaagaaca tcattttggg cgtgctgccc aacgttgttt
      961 cgtgagccag ccaacgcttt ccattgcgat taaaaaattg gaagaagagt tgggtgtggc
     1021 tttgtttgac cgcagcagca atgaaatacg tccaaccgat gctggtttgc gcattttgga
     1081 tcaagcgcaa cgggtgttgg aagaggcgga catgatcaag caattggcca aagaagagca
     1141 aaacgagttg gaaggttctt tcaaactcgg tttgattttc acggttgcac cgtatttgtt
     1201 gcccaaattg attgtgtctt tgcgtgactt ggcgccgaaa atgccgctga tgttggagga
     1261 aaaccataca gcggtgttga cagacatgtt gaaaaaaggg gagttggatg ccattgtggt
     1321 ggctgacccg tatcaagaag cgggtgtggt gaccatacct ttgtacgatg agccattctt
     1381 tgtgattaca ccgaaaggcc atccgtttga agaaatggat gaaatcacta cccaagagct
     1441 agcggatgaa caggtgttgt tgctcactga aggcaattgc atgcgtgatc aagtgttaga
     1501 cagttgtgtg gaattggcca gtcgccaacg catcaaaggt ttgaccaata ccttgcaagg
     1561 cagctccatc aataccattc gccacatggt ggcctcgggt ttgggcatca gtgtattgcc
     1621 agcaacctcg ttgactgaaa atgatcacat gttatttagt atcatcccgt tcaaagcgcc
     1681 tgcgcctgaa cgccgtgtgt tattggctgc tcgtcgtcat tttgtgcgtc aaaaagcatt
     1741 ggataccata caaaccgcta tttttcattc gcaattggca ggggtgagat tcgttaatga
     1801 ataaaattaa cgaaaacact tgacgcagtg cagcagatga actatagtta catttctgac
     1861 gcggggtgga gcagcatggt agctcgtcgg gctcataacc cgaaggtcgt aggttcaaat
     1921 cctgcccccg caaccagttt cagaaaaacc agcctaatgg ctggtttttt acatttcagg
     1981 acgcgcattt cagtggggct cagttacaat ggtggcataa ctgccatttt gcaaaacacc
     2041 acaaaggagc gctatgaata caagtgacaa caatccattt gccttgtggc aacaattctg
     2101 gcaaaacgcc aatccacaga tggccccatt cttgccaccg atgtctgccg aagaagtgca
     2161 gaagaaaatt actgaattgc aaagtgtgga actgtggtta agtttcaatt tgcaaatggt
     2221 acaatcgcaa ttggctgtgt tagagcagca aaaactgttc tttaattctc tcaatcaaat
     2281 gagtgaaaat ctcagcaaca ccaaaccaga ttaatctcca gcggctttac aggccgcttc
     2341 tattaggttg taacccatga cttccccacg ttatattccc ttgcgcatgc acactgagtt
     2401 ttccattgag gatgggatga tacgcatcaa agatgcggtc aaacatgcca gtggattggg
     2461 ctttgcggct ttgggtattt cagatttgat gaacctgttt ggcatggtca agttttacaa
     2521 agcctgtcgt ggggcaggca tcaagcccat tatcggggtg gatgtgtggg tgcaagacga
     2581 ccaaactgat gatggcgaca acaaacctta tcgtttgctg ttgattgccc gcaatcaacc
     2641 gggttttaag cgtttgtgtg aattgattac ccaagcctat gtggacgacg atcgcgacaa
     2701 aatggatttt cgtttgcgct attcttggct ggagagtggc gataacagtg gcttgatttg
     2761 tttgtctggt gcccatttgg gcagtgttgg tcagcatttg ttgtcaggcc ggcctgaaca
     2821 agccaaacaa gcggcttcgg ctttatccga attgttcccc aatcggtttt atttggaatt
     2881 gcaacgcttg cccgaaaagc ctgaatggga aaatgcggtg gtgggcagtt tgaatttggc
     2941 gcaagagttg gatttgccca tcgtggcgac ccatccagcc caatttctga ataaagacga
     3001 ctttcaagcc cacgaggcgc gggtgtgcat tgcctcgggt tatgtgttgg ccgatacccg
     3061 tcgccccaaa aatttttacc catcccagta ttttgcgcct gccgatgaat ttgaggcacg
     3121 gtttgccgat gtgcctgaag ccttgcaaaa ctctgtggaa atagcgaagc gctgcaatgt
     3181 ggaaattgtg ttgggtgaaa atttcttgcc gcaattcccc acacccgatg gcatgacgct
     3241 ggatgactac ctgtctcacc tatcaaatga gggtttggcc gaacgcatgg cggttttgta
     3301 tcccgatgaa accgagcggg ctcaacgcta tccagagtac caacagcgtt tggattatga
     3361 actcaatacc attgttcaaa tgggtttccc tggctatttt ctcatcgttc aagacttcat
     3421 taattgggca aaacaaaatg gctgccccgt gggcccaggt cgaggctcgg gagcaggttc
     3481 cttggtggcg tatgcactga aaatcaccga tttggacccg ttgaaatacg ctttgctgtt
     3541 tgagcgcttc ttgaatccag agcgggtgtc catgcctgac tttgacatcg acttttgcca
     3601 agccaatcgt ggccgtgtga ttcactatgt acgtgaccat tatggacagg atgcggtgag
     3661 tcaaatcgtg acgtttggca ccttgtcatc caaagcagtg atacgtgatg tgggacgggt
     3721 gttggatttg ccattcagca tgtgtgaccg tttgtccaaa ttgattccgt tggagcaaaa
     3781 caaaccgctc agtttgaaaa aagcgatgga aaccgagcca caaatcgccg aaatcattga
     3841 ggctgaagaa gctgatgagt tgttggtgtt ggcacaaaag ctggaagatt tgacccgtgg
     3901 cacgggcatg catgcgggtg gcgtattgat cgcacctggc aaaatcaccg atttttgccc
     3961 tgtttaccaa gcgcaaggtg aagacacatc tcctgtgtcc atgttcgaca aagatgacgt
     4021 ggaacaagcg ggcttggtga agtttgactt cttgggtttg cgcaacctga ccatcattga
     4081 aatggcgcaa aacttcatca aagatgtcac aggtgacatt gtggacgtga accacattcc
     4141 gttagatgac gcggatactt acaagcatat tttcgccgag ggcaatacca ctgcggtatt
     4201 tcaattcgag tcggcaggca tgcgccgcat gttgatggag gcggagccga gcaagtttga
     4261 agagatcatt gcctttgtgg ccttgtaccg tcctggtcct atggatttga ttcccgactt
     4321 tattcgccgc atgcacgggg aaaagtttga atacctgcac gagctgttgt ccaatgtgct
     4381 tgagccgact tacggcatca tggtgtatca agagcaagtg atgcaggccg ctcaagtgtg
     4441 cggtggctat acgctcggcg gtgccgactt attgcgccgt gccatgggca agaaaaaagt
     4501 cgaagagatg gtgaaacacc gcggcatttt tgtggaaggt gcggccaaga atggcattac
     4561 cgaaaaaaat gccaacgaga tttttgacta catggaaaaa tttgcgggct acggtttcaa
     4621 caagtcgcat gccgctgcct atgccttggt gtcttaccaa accgcttggt tgaaatacca
     4681 ttacccagct gaattcatgg cagccacgat gtcgtcagag ttggacaata ctgaccagtt
     4741 gaagattttt tatgacgatg ccaccagtcg catcaatggc attcgcttct tgcccccgtc
     4801 agtgaatgct tcgttttacc gtttccaacc agaagcaggc aaaaaaatcc gctatgccct
     4861 cggcgcgata aaaggcacgg gtgaggcagc tgtgaacgcc atagttgaag cgcgtgaaaa
     4921 aagtggcgca ttcaccagct tgtttgattt ctgcgaacgg gtgggcaagc aacacctgaa
     4981 caaacgcacg gttgaagctt tgatacgcgg cggtgctttt gacgaaattg agcccaatcg
     5041 cgccatgttg ctgggcaacg tgggtttggc aatggaatat gccgaccaaa aagccgccaa
     5101 tgccaatcaa ggaggcttgt ttgatttcat cgacgatgcc agcgccatgc cggaagtgga
     5161 aatggagcct gtggcagcct ggtctgaatc agacaaattg gccgaagaaa aacaagcctt
     5221 gggcttttat tattcaggcc atccgttcac gccgtaccgc gccaccatac gtggctttgc
     5281 caagaaaaat ttggccgaca tcaagcctgc cgaccacaaa caatgggtgg ggggctttgt
     5341 cacaggtgtg cgcaccatca tgggacgtcg cggcaaaatg gccatcgtca ttgtggaaga
     5401 cgaaacccaa aagcttgaag tggtggtggc gggcagctcg tatgagcaac accaacacct
     5461 gctcaaaacc gaccaagtgc tgttgtttga atgcaaagtg gcgcgcgatg agtataacgg
     5521 tggcggcggt gtacgcatca attcggatgg tatttacgat tttgatggcg cacgcagcgc
     5581 gtttgcacgc agcctgtgtt tgaccatgac accaaacacc gatgtggtga aactctcggc
     5641 cttgttgggc aatagccgtg gccaaggtta tgtgggcctc aaagtatctt acgaacaccc
     5701 agaggtgagc gccgaactca agcccaaatc gccgtggcaa atcggcatag acagcgcttt
     5761 gctcaagcag ctgagtgagt tattgggaca agatgcggta gaagtggtgt ggtgatgagt
     5821 ggttaatatg cggcctgtaa acgatttaca ggccgcataa gtaattttgg taagggtgag
     5881 tatgaacgaa gcgtcttcaa ggcaattggt ctgttttgta gaacaatgga ctgcgataca
     5941 acagcattca gatgagccat tgcaaaataa attggctatt ttttttcaaa agtgggtatt
     6001 ggaaactact gagcttacag atgtcatcaa gccagaggcc attcaacatg atattaatca
     6061 tctagcgctg tctcattttt ttaatgcgta ccagcatgcc agacagccta tgttggaagc
     6121 catgaagcaa ggcatgtttg tcaatgtttg gcaaacggca ggtttgaagc atgatgaggt
     6181 tcgtaacagc aaagtattac agtggttttt aaacccgaat ggaaatcatg gtcaaagcga
     6241 tgcattgctc aaacaattta tcacgttatt accaattgaa tttcaaaatc tggacacgca
     6301 aagcagccga gtgattgcag aaagctgtcc attgggagaa ggcactgaac gcgtagacat
     6361 tgaagttgat gcacagcatt ttttgctgtt tattgaaatc aaaatcaatg cctatgaagg
     6421 gcaagagcaa ttaaatcgat atttgaacat tgcgcaacga aaagcacaca acaggccatg
     6481 gatggtggtg tatttaacca accaacctca attacctgtt atgcatcgaa acaagcaaaa
     6541 tttaatcgcg atttcatggc agcaagtggc caaagctttt ttgcaatatt ccaaacaggc
     6601 tgaaacacac aatcgaagtg catggttggt tcagcaattt gctgaacata tccaagcttt
     6661 ttaaaattta ggggaaagta tgagtaacaa acaagcaggt aaattaatca tagaaaacat
     6721 tcaaatgctt gatgaggcag taaagctatt aagtggagat ttatctcaac agatttatga
     6781 agggatagag aaagttttac aagataaatt gagtgaattt tcagaggagt ggagccgaag
     6841 caatgaattg ttatcagatt tttcctatcc atttgcgcct aaaaaatgga gactgattga
     6901 ttctgaacaa tttaggcaca aagattattt tgcccgttat tgcctgactt gggaaagcac
     6961 tcattcagaa caagaaacaa atcagtattt gctaactgca ttttttaaaa attcttatga
     7021 tgtcataatt tttaaatttt ttccttggtt tggctgctat gcacgatgta caaatggttt
     7081 gtggaaaaaa tttgcaactg cccagaatga aaaatatcct gaaatcgaaa gtttgggatt
     7141 taaatactct gctaaagaag gtgaatggta tttaccgatt gacagcttga gtcaacaatt
     7201 ggtggctgat tgttatgtgg aagataattt ggccgatgca atgggaccca ttacagaggc
     7261 tttggagaag ttgtatcaag cccatcccta ttttgacaag attgttgaag ccgccaaagc
     7321 cgaatttggt gtgaatgtgc cagattagtc ttaaactgca taacaaagcg gcctgtaaat
     7381 catttacagg ccgctttgtt atgcagttta ttttatgaga atgaaatagt tggttttcat
     7441 cttgcttgct ttaatgtaga gcattcaaat aggagatgtg atgaaaacca tacgttcagc
     7501 cattgaaatt gcacggccac aggaagtggt gtttgacctc acgcaaaact acgccaaacg
     7561 cttggaatgg gatccttatt tggccgaggc ctatttgatg aaagatgcgg ctgagcccgc
     7621 agtgggtgtg gatgcatatt gcaaaaacca caatggctcg gtgatggtgt cgcgctatat
     7681 ttcatttaac cgccctgcgg tggctgcggt gaacatggta aaagggccca aaatcctcaa
     7741 gcgcttcagc ggggcttgga atgtgcacaa aatggatgat caacattcag aactgatttt
     7801 cacctaccat tttgatttgc gtggaggcat agcgggggaa atgatcacgc cgattgtgcg
     7861 ctggcatttt caacgcgaaa tgaacaaacg gctgtcagcc tttaaggcgt atttggaagc
     7921 cgaatcggct tgatggtcat gtctgtatcg atagaaattt gtttctgggg gtcatgatgg
     7981 gttttttgca aaaattaatg ggcaaacatc aagccattgt gttggataaa gtggtgcacg
     8041 acaaagacac tttttgggca tggtttcaaa cgcaagcagc ggcgttccat catgctgtca
     8101 tcacccaaga aaaaattgac actgattttt ttgacccact ttcagccagt ttggatcgca
     8161 tgcacgaagg catttatttt ttggcaggtg aagcagaggc tggtgtggcg gagctgattt
     8221 tgaccgccga tggtgtgtac cgcaattttg cgtttattga ggatttggtg gccgctgcgc
     8281 ctaaattacc tgcttggcgg tttgtggctt taaagccagc ttgtgcgatt gatggatttg
     8341 gcatcaacat gaatgggctg gtttacagcg atgacacttt gcatttttat gtgcgcgaag
     8401 atgccaaatg tccacaagtg gtggatgtgg tgatggtaca cagcgatgcc acggccacag
     8461 aagaacagct caccagcggt gtgtatgtct atttggacaa tttattgggc gaatgggtgg
     8521 cggtcacaca aatagaccag atgcaaatca tcaatcaaca acagcttcct gcaaaccaaa
     8581 ccttgcagcc gatggccgaa ttggcgccgt atttacacag caggcatgag gcttggcaaa
     8641 aagcgtatgg cggcgtgtat tacaacagcg atcaggacga attcgcgagt tttgagggcg
     8701 aggccgacaa tggacagctg attgtcggca tcattgacag caccttattg gcatggcctc
     8761 ataagccgtc gcatccttgg atgctgacac tgcgcattgc ctatgacggc agtggtttga
     8821 atggcatgcc ttcaaaacag atataccaac gcatggagga gtttgaggat gccttgatgg
     8881 ccgaattgcc agaacatgaa ggctatttga atgtgggccg tgaaactggg gacgatattc
     8941 gttacattta tttcgcctgc aaacaatttg tgcaagcgac aagggtattg gagcgattga
     9001 tgccgcaatt cagccaggag atggacatca gctatgatgt gtttcaagac aaatattggc
     9061 agtctttgca gcggtttgaa cacctgagca gtgcagaaga ttgagagtaa atcttaaaag
     9121 ctgagacaca taagcggcct gtaaaccatt tacaggccgc ttattcaatt caggttcact
     9181 ataaacgctt gcccaataac accacgctga cctccttgtc atccaattca cacacattgg
     9241 gtaaagtgcc ccacaaactg aagccatttt tttcaaacat gcgcacactg ggttggttgt
     9301 gggcaaaaat caccgcgatg acattgcgta tgcccaatga tggggccaat gccaacatgt
     9361 aatcgagcag ggtttgcccc aagcgtttac cgcgctgaga ttcatccaca taaatgctga
     9421 tttcggcact gatgtcataa gcggcgcgag catagtaatc gctgaaagtg gcccaagcca
     9481 aaacgcagtc ttgttcatca cacaacacca tcatggggcg gtggggctgt tggtgggcat
     9541 cgaaccagtt gaggcgctcg tctacgctga caggctgcaa atcggcagtg acttggcggc
     9601 ctgcaaccgt gctgttgtaa atggccacga tggcgggtaa gtcgctggca tcggctgggc
     9661 gtatgggcat ggtgtgtatc cttgttacaa cattgaaata aggccaacag tagcagaata
     9721 aggaaagagg gtggttactc taaaatttca aacacttgtt tgaaaaaacg tgattcaata
     9781 gcggaatcca tcaaaacaat accaaaacga tggcaagttt ttcccaacat tggagatgaa
     9841 aaatgatgaa acatgtttta ttgctcagta tcttgtgttc agcaggcttg accatggcac
     9901 aagacatcag tggcaagtgg cgcaccatcg atgacgaaac caaaaaaccg aaagccgtgg
     9961 tgcaaatcag caaaaatggc gacagctatt caggccgcat tatcagccta gaggcgggtg
    10021 tggaaaacaa atgtgcgggc tgtagtggcg cgcaaaaagg cggccctttg gtgggcttga
    10081 ccgtggtacg tggcctgaaa gaggatggca aagcttacag cggcggcagc atttttgatc
    10141 ccaaatcagg caaaacttac aaagccaatg ccgaattggc caatggcggc aattcattga
    10201 aagtgcgcgg ctatgtgggc gtttccgcct tgggccgcac ccaaacttgg caacgggttc
    10261 agtaaacaga tgcaaaaata agtaaggcca cct
//
LOCUS       DAOMVK010000017        10239 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_1600_length_10239_cov_3.048606, whole genome shotgun sequence.
ACCESSION   DAOMVK010000017 DAOMVK010000000
VERSION     DAOMVK010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 10239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10239
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1600_length_10239_cov_3.048606"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..881
                     /locus_tag="PK856_01320"
     CDS             57..881
                     /locus_tag="PK856_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HRL01041.1"
                     /translation="MLFSSAVRYSAAVLALGLGLSACGGQSADTAKAASSAPAAASDA
                     APAAGKTYKIALNAEFAPFESITSEGKMEGFDIDLMDALGKAGGFQVEYKHQPWEGLF
                     TTINTGDADAIISAVTMTDERKQTMAFTDPYYTITQIVLVPQGKSVASVNDLKNLDKV
                     GVVTGQTGDLAVQKILGATSDKIVRYETFALAAKEIENGSVDAVVSDSAVVANYVKLN
                     SSKGFTMVKVPDFTEEHYGIAVAKDNQELLTVFNAALKQVKDSGEYQKIADKYFAH"
     gene            956..1786
                     /locus_tag="PK856_01325"
     CDS             956..1786
                     /locus_tag="PK856_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003791415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HRL01042.1"
                     /translation="MLFTQFKGMGLALAAASILAACGGQSSSSSTAPAASAPASASAT
                     STASVDGKEYKVAMNAQFPPFESMNSSGQIEGFDVDVMNAIAKEEGFKVTYHQQAWDG
                     IFASLKAGDNDILISGITITDDRKKEMLFSEPYYKITQVVLVPQGKNIASVEDLKKMD
                     KVGVTIGTTGDLAAQKILGATSEKIIRLENLPLMLKEVETGAVDAVISDSAVVADYVK
                     KKGDKGFTMVEVPDFTVEYYGIAVRQDDKALMDKINSGLKKIQASGEYDKISDKYFAK
                     "
     assembly_gap    2399..2455
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2456..3084)
                     /locus_tag="PK856_01330"
     CDS             complement(<2456..3084)
                     /locus_tag="PK856_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRL01043.1"
                     /translation="MENSFKSRLLFLWQDTKPAQIAKDIGLTISGVLRILEKDTMPKA
                     ETLIRIKTLKKCDWNWLMTGEGDPFPDAQHSQLQVNKDIPSHIPMVYDTLGYEVDINE
                     FVFIPRYNVHAAAGGGHFNDVEKPMFTMAFRRKWIEQYLRADPKELSVIDVKGDSMDG
                     VLNERDVILVNHYLNTPDDGLYVLRIGENLIVKQTQQLPNGRLLVKSANP"
     gene            3314..3586
                     /locus_tag="PK856_01335"
     CDS             3314..3586
                     /locus_tag="PK856_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRL01044.1"
                     /translation="MQKKSASIVLADWHPADIKAELNKKGLTLRALSLANNMHQDSLK
                     TALRTPYPKGERIIAAALGYEPEEIWPNRYAKRMFKAVIPPQAINA"
     gene            3647..4132
                     /locus_tag="PK856_01340"
     CDS             3647..4132
                     /locus_tag="PK856_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01045.1"
                     /translation="MRNGKRISQKHKATSLDHAIELTEEYAEQYRMPSKRIAELMGVE
                     YKTYRRWMLDGTLPLNRLVTLEHIVGAQFISEYLCVFQGNKVVIDIARGKKSDVVDIA
                     ELQAQIAQSMACLAQFYAGKANADDTIAELTASITSLAYQRENVRKVETPELLLGDRD
                     E"
     gene            4440..6191
                     /locus_tag="PK856_01345"
     CDS             4440..6191
                     /locus_tag="PK856_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HRL01046.1"
                     /translation="MALAIDEANGLNDKQRDIAHARMAIVAEVNKMHQVGGLPLKEAA
                     FYVTEQIKTGVAPESLMYMVEVANARGNKKRTVSFSSLYQWTLKFREAKTPNERLLAL
                     APKLSSVEKPLIECEWLPEFMRFWAKPNHPTQTQAYKDFKEQWQKKGMEMGKLPSVDT
                     VSRTLRKMPLIMRERGRKTGGAYKAIMPYVKRDWLIFEPNDIWVGDGHGFKAKVAHPI
                     HGQPFQPEITAIIDTATRFVVGWTVSLSESTVAHADALRVAMSFNDPPLMYYTDNGSG
                     PTGKMVDADITGILPRLGIEHPTGLPGNPQGRGIIERLWETVTIPLARTYATYVGHDA
                     DNSTKTLTLRKVNSAMNAENKRKELSPEQRRYRAMLPTFQQFMVDLAKAFDDYNNRHE
                     HSELMKASNKQYGTPAAYRAAKLQALGKTLVPLSQDELDMMFRPEEIRTANRGQVELF
                     TNIYFSQGLAEFGGEKVRVGYDLHNPEMVLIKRMDGQFICKALWDGNKVAAFPVSVID
                     QQRKKRVEGITKRKQDAIAIAQAELYPTIEHQPDFGLLLANGANEVETVSVQKPPLFM
                     FESEREAWEIEQRNKSK"
     gene            6213..7148
                     /locus_tag="PK856_01350"
     CDS             6213..7148
                     /locus_tag="PK856_01350"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRL01047.1"
                     /translation="MKRTNKSLTANTQVESIRLELQRYIDEHGISQVTVARGIGKTHP
                     VISQFLNNKYPGDVEEVARAIESWLTKQHEKAASKKVAINYVQTSVAKKVREILRIAH
                     VEAETVALIGQAGLGKSTALKAYSSENPDAILIDTDPTFTAKVMMQTLAASIGEDSHL
                     PLHDLNENIINRLKGSGRIILVDEAENLPLRALECLRRLHDKAGVGLVLAGMPRLMIN
                     LRGKNGELKQLYSRVAFKLDMGEAIPDSDLRMIVDQALPGIEEDVALEFVAAADGNTR
                     KLSKLIGGVTRLARINDNADISPDMVRQFKEMLIH"
     gene            7162..7404
                     /locus_tag="PK856_01355"
     CDS             7162..7404
                     /locus_tag="PK856_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01048.1"
                     /translation="MKALIAKLLANICQRQIQYNQDQWQLIDAALSYEMRNMPVYMVQ
                     ERVATRQNAFEMQRLHTQKRVDWSKRLNRIQQWAKG"
     gene            7516..7989
                     /locus_tag="PK856_01360"
     CDS             7516..7989
                     /locus_tag="PK856_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01049.1"
                     /translation="MKELKFTPEMQVAILLGKKQQTRRIVKPLNGGEIIGHGGVGLAM
                     ELDGEELDEDGEAISLVNTVPCPYGQVGDVLNAPQFPCLKLQITEVRVERLQKITEQD
                     CLKEGVGNPVLRQCNQSEFIALWNAIYGYQAWDVNPYVWVIEFEKTEQLNGECNG"
     gene            7982..8680
                     /locus_tag="PK856_01365"
     CDS             7982..8680
                     /locus_tag="PK856_01365"
                     /inference="COORDINATES: protein motif:HMM:NF022432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HRL01050.1"
                     /translation="MDKKTALAKIKKCLALAKSANEHEAAQALKQAQALMREFEVTEQ
                     DVEWSQVNEASQPYNSRCVMWQVGLVNMCAKAFGCSQFSHTKWDNGQFKTRINFVGIG
                     ARPELAAYAYEVLLRQVRKARSEYRKTALSRLRKTKTINYRLDAFSVGWVRSVRDLVT
                     TFASSPTEQAILNGYMKKNYPDLIEQEVKPKSATSALKTAANMDYLHGQKAGESAQLN
                     HAMGHSPKQIGFHS"
     gene            8677..9165
                     /locus_tag="PK856_01370"
     CDS             8677..9165
                     /locus_tag="PK856_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019390191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01051.1"
                     /translation="MKTRCPCCGAENSLDALLNHDAARLAMWELTKMGDEMTRLAVNY
                     VGLFRPAKSTLSFDRMAKLLEELRVQMDDGFIERDGQRIEVSRAAWAYGFRVMLEKRA
                     TGLKLPLKSHGYLYEVLASYQAPATAVAQKEALAITGKSSQTMAGLAALESMKYGGSI
                     SK"
     gene            9143..9571
                     /locus_tag="PK856_01375"
     CDS             9143..9571
                     /locus_tag="PK856_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01052.1"
                     /translation="MAVPFLNEAVITGLQRLLALRLVGSPPADAIELTASVWIDAMDR
                     YPIAWNEQLDGWRINAGFDQLVWQSERWPTVKHLLDSLPKRIPPPPLPKPAMTKQMQK
                     EGKKKLQQILNELETKRMPPPHDGRQALNQAIETMKLKKD"
     gene            9574..10116
                     /locus_tag="PK856_01380"
     CDS             9574..10116
                     /locus_tag="PK856_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003789995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0042262 - DNA protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HRL01053.1"
                     /translation="MLMKASKTRRKTEAAPAITQTRDEVIENIKRMGDLVRNRERVQA
                     EMNDAIAQVQERAAEEIAPLDIELQELEASILMYCTTNRDVLTDGGKVKFADLITGKV
                     QWRNNPPKVQIRGNDAVLALLEQDEKYERFVRTKREVNKEAVLNEPDFFAGNPVPGLS
                     IVQGKEFFVIEPYNQQLPEA"
BASE COUNT         3039 a         2098 c         2442 g         2603 t
ORIGIN      
        1 atgttaacca tctggccgac ctcaaggttg gcgtttgttt tttaatggag tgaaatatgt
       61 tgttctcgtc tgctgttcgt tacagcgcag ccgtattggc tttgggtttg ggtttaagtg
      121 catgtggtgg tcaatctgca gataccgcta aagcggcaag cagcgcacca gctgctgcca
      181 gtgatgcggc acctgctgca ggtaagacat acaaaattgc gttgaatgcc gaatttgccc
      241 catttgaatc catcacgtct gaaggcaaaa tggaaggctt cgacattgat ttgatggatg
      301 ccttgggcaa agcgggtggt ttccaagtgg aatacaaaca ccaaccttgg gaaggtttgt
      361 tcaccaccat caacacgggc gatgccgatg ccatcatttc tgcagtcacc atgaccgatg
      421 agcgcaaaca aaccatggcc ttcaccgatc cgtattacac cattacccaa attgtgttgg
      481 tgccacaagg caagagcgtg gcctcggtca atgacttaaa aaatttggat aaagtgggcg
      541 tggtaacggg ccaaacgggc gatttggcgg tgcaaaaaat cttgggcgcg accagtgaca
      601 agattgtgcg ttacgaaacc tttgctttgg ctgccaagga aattgaaaat ggttctgtgg
      661 acgcggtggt gagcgacagc gccgtggtag ccaattatgt gaaactcaac agcagcaagg
      721 gttttaccat ggttaaagtg cctgatttta ccgaagagca ttatggcatt gcagtggcca
      781 aagacaacca agaattgttg accgtgttca atgcggcttt gaaacaagtc aaagacagcg
      841 gcgaatacca aaaaattgcc gacaaatatt tcgcgcactg atgtgaattc gggtaacctg
      901 tttttgaggt tgcccttatt tttaaccaca atacaacact acatagggag aaaacatgtt
      961 attcacacaa tttaaaggca tgggtttggc attggcagcg gccagtattt tggctgcttg
     1021 tggtggtcaa tcgtcatcta gtagcacagc gcctgctgcg agcgcacctg cttcggcatc
     1081 tgccacttca acggcaagcg tggacggcaa agagtacaaa gtggcgatga atgcccaatt
     1141 cccaccgttt gaatccatga attcgtctgg tcaaatcgaa ggctttgacg tggatgtgat
     1201 gaatgcgatt gccaaagaag aaggcttcaa agtgacgtat caccaacagg cttgggatgg
     1261 tattttcgcc agcttgaaag ccggtgacaa cgacatcttg atttctggca tcactattac
     1321 cgatgaccgc aaaaaagaaa tgttgttctc tgagccttat tacaaaatca cccaagtggt
     1381 tttggtacca caaggcaaga acattgcttc ggttgaagac ttgaaaaaaa tggacaaagt
     1441 cggtgtgacc attggtacca cgggcgactt ggccgcgcaa aaaatcttgg gcgcgaccag
     1501 tgaaaaaatc atccgtttgg aaaacctgcc tttgatgctg aaagaagtgg aaaccggtgc
     1561 ggtggatgcg gtgatttctg acagtgcagt ggtggccgat tacgtgaaga aaaaaggcga
     1621 caaaggcttc accatggttg aagtgcctga ctttaccgtt gaatattatg gcattgcggt
     1681 tcgccaagat gacaaggctt tgatggacaa aatcaactca ggtctgaaga aaatccaagc
     1741 cagcggtgag tatgacaaga tttctgacaa gtattttgcc aaataatcct ttgctttaag
     1801 tagcaaaaat cgattcactg cgtcctgtaa attgcactga ttgtgttctc tatggtcttg
     1861 tgacctgttt tagggaatag tgcgatttac aggccgcttt tatttggcaa actcaaagaa
     1921 taaatagtaa caagtcggtt aggtaacgta ttgatgcaca attaatttat gtgtgttgcc
     1981 ccaaacccgc taaactgatg ataaaatgcg gcgtgaatgg aataattaat ccaatttaac
     2041 aattgatttg gcttaaggac aaacgatgaa aacgatgtta aaactgatgg cagcgactgt
     2101 aatcatgtgg gggggtgtcc gcttttaaac cagagtgaga gtaaataaaa atggcctgaa
     2161 atccttaact gtattggatt tcggccattt tattttgtat tgttacacgc attgcctttt
     2221 aaaccagaat aggcaaatat tgatattatt ctgaaatata atgagcaggt tttaaactga
     2281 agtaagcaaa ttttaaacag atttaaaccg gtgttaaaag gtatttaaac ttgtcggcca
     2341 aaccaaacca ctctgccaat aatttttacg tcggtgtctg tttcgagtag atttatttnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggatt
     2461 ggctgactta actaataagc ggccattcgg cagttgttgg gtttgtttca caattaagtt
     2521 ttcacctatt ctgaggacgt ataaaccgtc gtccggtgta ttcaagtaat gattcaccaa
     2581 aatcacatca cgttcattta aaacaccatc catgctgtcg cctttaacat ctatgacgga
     2641 aagctcttta gggtcggctc ttaagtattg ttcaatccac ttacggcgaa atgccattgt
     2701 aaacattggc ttttctacat cattgaaatg cccgccacca gcagcagcat gtacgttgta
     2761 gcgaggaata aatacaaatt catttatatc cacttcataa cctaatgtgt cataaaccat
     2821 aggaatatga gagggaatat ctttatttac ttgcaactga ctgtgttgtg catccggaaa
     2881 tggatcacct tctccagtca tcagccagtt ccagtcacat ttttttaagg ttttaatcct
     2941 aattaatgtc tctgcttttg gcatcgtatc tttttcaaga attctcagca ctccactaat
     3001 agtcaaacct atatctttag cgatttgagc tggttttgtg tcttgccata ggaataagag
     3061 tcgcgattta aagctatttt ccatatatca gcccataaat tcttttgttt agaaggtgca
     3121 tcgttactaa acaaaaaact aaacaaaaga gttcgttttt tgtttagtaa atttactttt
     3181 aagattcaat atcttgcaaa ttaactgtac aaaaaagaaa ctttaactta acaaaagatt
     3241 tgcattgtac ttttttgttt agtaatatcc cctacagata acccgtcaag ataacccaca
     3301 caggtgaatt actatgcaaa aaaaatcagc ctctattgtg ttagccgatt ggcacccagc
     3361 cgatattaag gctgagttaa ataaaaaagg tttaacgcta cgtgctttgt ctcttgcaaa
     3421 caatatgcat caagactctt taaaaacagc cctgcgaact ccctatccaa aaggtgaacg
     3481 catcattgct gcagcacttg ggtatgagcc tgaagagatt tggcctaatc gatacgcaaa
     3541 acgtatgttt aaagcagtca ttccacctca agcaatcaat gcctgaatga tagtggattt
     3601 actaaacaaa tgcacataaa acgattcgca cactaaggtt ttgatgatga gaaatggcaa
     3661 acgaatttcg caaaagcata aagcaacatc gcttgatcat gcgattgagt taacagaaga
     3721 gtatgcagaa cagtatcgca tgccttccaa acgcatcgct gaactgatgg gtgttgaata
     3781 taaaacatat cgccgctgga tgctggatgg cactttaccg ttgaaccgct tggtaacact
     3841 tgagcacatt gttggtgcgc agtttatcag cgagtaccta tgtgtgtttc aaggcaacaa
     3901 agtggtgatt gatattgcac gaggcaaaaa aagcgatgtg gtggatatag ctgaattaca
     3961 ggcacaaatt gctcaatcaa tggcatgttt ggctcagttt tacgcaggca aagccaatgc
     4021 agacgacacc atagcagagc ttacagccag catcaccagc ttagcttacc aacgtgagaa
     4081 tgtgcgcaaa gttgaaacgc cggaattgtt gttaggtgat agagatgagt aataagtttt
     4141 cctccgctga gttagccgaa atgaacttac tgaacatgcc aaaaagtaag caagctattg
     4201 aatatattgc gaagcgtgat ggttggcaat atgagtatgt gcttggtttg gggcgtggag
     4261 gtaagaaaaa agtatttttg ttaaatggct taccaatgga attacaggat gccattaagc
     4321 aaaaacaact tgatgacctc atgcaaaaaa gccagccagc accattgcca agtgttgtta
     4381 agaaaaagca gtcacctaca aaaaagaagc aattaccagt tagtaaaaag agccaacagt
     4441 tggctttggc aatagatgaa gccaacggtt taaacgataa acaacgtgat attgcccatg
     4501 cccgtatggc cattgttgct gaggtcaaca aaatgcacca agtaggtggt ctaccgctta
     4561 aagaagcagc tttctatgtg actgagcaaa tcaagactgg tgttgcacct gaatccttga
     4621 tgtacatggt tgaagtggcg aatgcccgcg gcaacaaaaa gcgcacagtc agttttagca
     4681 gtttgtatca gtggactttg aagtttcgtg aagccaaaac acccaatgaa cggttgctgg
     4741 cattggcccc aaagctaagc agtgttgaaa aaccgttaat tgaatgtgag tggttgccag
     4801 agtttatgcg gttttgggcg aaaccaaacc atcccacaca aacacaggca tacaaagact
     4861 ttaaagaaca gtggcaaaag aaaggaatgg aaatgggcaa gctgcctagc gttgatacgg
     4921 tatcacgtac tttgagaaaa atgccgttaa tcatgcgtga acgtggccgt aaaaccggtg
     4981 gcgcttacaa agccatcatg ccctacgtta aacgtgattg gctgattttt gaaccaaacg
     5041 acatttgggt gggtgatgga catggtttta aagccaaggt ggcacatccc atacatggtc
     5101 agccgtttca gcctgaaatc accgcaatca tcgacacagc cactcgattt gtagtgggtt
     5161 ggacggtgag tttgtcagaa agcactgttg cccatgctga tgcactgcgt gtggccatga
     5221 gctttaacga tcctccgctg atgtattaca ccgataacgg tagtggtccc acgggcaaga
     5281 tggttgatgc agacatcaca gggattttgc cgcgtttggg aattgaacac ccaacaggct
     5341 taccaggcaa tccacaaggc cgcggcatta ttgagcgctt atgggaaacc gtaacgatac
     5401 cgttggcacg tacctatgcc acatatgtgg gccacgatgc cgacaactca accaaaaccc
     5461 tgacattgcg caaggtaaac agcgccatga atgcagaaaa caaaagaaaa gaattaagcc
     5521 ctgagcaacg ccgttaccgc gccatgttgc ccacttttca gcagtttatg gtggatttgg
     5581 ctaaggcttt tgatgattac aacaatcgcc atgagcacag cgaattaatg aaagccagta
     5641 ataagcagta tggaacgcca gcagcgtacc gagctgccaa gttacaagca ttgggcaaaa
     5701 ccttggtgcc tttaagccaa gatgaattgg acatgatgtt taggccagaa gaaatccgca
     5761 cggccaatcg tggacaagtt gagctgttta ccaacattta ttttagccaa ggtctggctg
     5821 agtttggcgg cgaaaaagtc cgcgttggct atgacttgca caaccccgaa atggttttaa
     5881 tcaagcgcat ggacggtcag tttatttgta aggctttatg ggatggaaac aaagtggcgg
     5941 catttcctgt ttcggttatt gaccagcaac gtaaaaaacg tgttgagggc atcaccaaac
     6001 gtaagcaaga cgccattgcc attgcccaag cagagctata cccaaccatc gagcaccaac
     6061 ctgattttgg tttgctgtta gcaaatggtg ccaatgaagt agaaaccgta tcggttcaaa
     6121 agccaccgct ttttatgttt gaaagtgaac gtgaagcttg ggaaatcgaa cagcgtaaca
     6181 aatcgaaata acacttactc aggaaaatac aaatgaaacg taccaataaa agcctcacag
     6241 ccaataccca agtggaaagc attcgacttg aattgcaacg ctacattgat gagcacggca
     6301 tttcccaagt cacagtggca cgcggcatcg gcaaaaccca ccctgtgatc agtcaatttt
     6361 taaacaataa atatcctggt gatgttgagg aagtggcccg tgcaattgag agttggttaa
     6421 ccaaacaaca cgaaaaagcg gccagtaaga aagtggcaat caattatgtt caaacatcag
     6481 tggccaaaaa agtgcgtgag attttgcgta tcgcccacgt tgaagctgaa accgtggcat
     6541 tgattggcca ggctggtttg ggcaaatcca ctgcattgaa agcctattca agtgaaaacc
     6601 cagacgcaat tttaatcgat accgacccga cttttaccgc aaaagtgatg atgcaaacct
     6661 tggcagcatc cattggtgag gactcgcacc ttccattgca tgacttgaat gaaaacatca
     6721 tcaatcgttt gaaaggttca ggccgcatta ttttggtgga tgaagcagag aacttaccct
     6781 tgcgtgcatt ggaatgtttg cgccgtttac atgataaggc tggagtgggt ttggtcttgg
     6841 caggtatgcc acgcctgatg attaacttgc gcggtaaaaa tggcgagttg aaacagcttt
     6901 acagccgtgt ggccttcaag ctggacatgg gcgaagcaat accagattca gacttgcgca
     6961 tgattgtgga tcaagcattg ccaggcattg aagaagatgt cgccttggaa tttgtcgcgg
     7021 cagcagatgg caacactcgc aaattatcca aattgatagg tggtgttacc cgtttggcaa
     7081 gaatcaacga taacgccgac attagcccag acatggtgcg ccagtttaaa gaaatgttga
     7141 tccactgata aggagctgca aatgaaagca ctgattgcaa agttattggc caatatctgc
     7201 caacgccaaa ttcaatacaa ccaagaccag tggcaactga ttgatgcggc tctttcttat
     7261 gaaatgcgca acatgccagt ttacatggtg caagagcgtg tggccacgcg ccaaaacgcc
     7321 tttgaaatgc aacggttgca cacacaaaag cgtgttgact ggagtaagcg tttgaaccgc
     7381 atccagcaat gggcaaaagg ataaagccat tatgagattc atacgcactt tatggtttta
     7441 cttttgtttg ttcgtgattt catcgccgtt tttaatttgt tttgtttggt tggttttggt
     7501 gcttaaggat ttgttatgaa agaactcaaa tttactccag aaatgcaagt ggccatcttg
     7561 ttgggcaaga aacaacaaac ccgccgaatt gttaagcccc ttaatggtgg tgaaattatt
     7621 ggacatggtg gtgttggctt ggccatggag ttggacggtg aagagttgga tgaagatggc
     7681 gaagcaattt ctttagtcaa caccgtgcca tgtccttatg gccaagtggg tgacgttttg
     7741 aacgcgccgc aattcccttg tttgaaattg caaattactg aggttcgtgt tgagcgtttg
     7801 cagaagatta cggaacagga ttgtttaaaa gaaggtgttg ggaatccagt tttgcggcaa
     7861 tgcaatcaat ctgaatttat agccttatgg aatgccattt atggctatca ggcttgggat
     7921 gttaaccctt atgtgtgggt aatcgaattc gaaaaaactg aacaattgaa tggtgaatgt
     7981 aatggataag aaaacagctt tagcaaaaat caagaaatgt ttagcactgg ccaaatcagc
     8041 caatgagcat gaagcagctc aggcgttgaa gcaagcacaa gccttgatgc gtgaatttga
     8101 agtgactgag caagatgtgg aatggtcgca agtgaatgaa gcatcacagc catacaacag
     8161 cagatgtgtg atgtggcagg taggcttggt caatatgtgt gctaaggcgt ttggatgttc
     8221 tcagtttagc catacaaaat gggacaacgg ccaatttaaa acccgtatca actttgtggg
     8281 cattggggca cgcccagagc tggctgctta tgcttatgag gtgttattgc gccaagtacg
     8341 caaagcacgg tctgagtatc gtaaaacagc tttaagccgg ttacgcaaaa ccaaaaccat
     8401 taactaccgc ttggatgcct tcagtgttgg ttgggttcgc agtgtgcgtg atttggtgac
     8461 cacctttgca agttctccaa cagagcaagc cattctaaac gggtacatga agaaaaatta
     8521 cccagacttg attgagcaag aagtaaaacc aaaatcagcc acatcggctt taaaaacagc
     8581 agccaatatg gactatttgc acgggcaaaa ggcaggtgaa agcgcacagc ttaaccatgc
     8641 aatgggccat tcacctaagc aaataggatt tcactcatga aaaccagatg cccatgctgt
     8701 ggggctgaaa acagcttaga tgctttgtta aatcacgatg cggcgcgttt ggccatgtgg
     8761 gaattgacga agatgggcga tgaaatgacc cgtttggccg tgaattatgt cggtttgttt
     8821 cgtcccgcca agtccacatt gagttttgac cgtatggcca agttgcttga ggagttgcgg
     8881 gtgcagatgg atgacggctt tattgagcgt gatgggcaac gcattgaggt tagccgagca
     8941 gcttgggcct atgggttccg ggtgatgtta gaaaagcgtg ccactggttt gaagctgccc
     9001 ctgaagtccc atggttattt gtatgaagtg ttggccagct accaagcccc tgcgacggca
     9061 gtagcccaaa aagaagcttt ggccataaca ggaaaaagca gccaaaccat ggcaggactg
     9121 gctgcactgg agagtatgaa atatggcggt tccatttcta aatgaggcag tcataacggg
     9181 tttgcaaagg ctgcttgcct tgcgcttggt gggttcaccc cctgcggatg cgattgagtt
     9241 aactgcctct gtttggatag atgccatgga ccgttaccca attgcttgga atgaacagct
     9301 ggatggttgg cgcatcaatg ctggatttga ccagctggtg tggcaaagcg aacggtggcc
     9361 aaccgtgaag cacttgttag acagcctacc caaacgaata ccaccaccac cattgcccaa
     9421 accggccatg acaaaacaaa tgcaaaagga agggaaaaag aaattgcagc aaattttaaa
     9481 tgaattagaa accaaacgaa tgccaccacc ccacgatggt cggcaagcct taaaccaagc
     9541 cattgaaacc atgaaactga aaaaggactg attatgttga tgaaagcctc caaaacccgc
     9601 cgtaaaaccg aagccgcgcc agcgattacc caaacccgtg atgaagtgat tgagaacatc
     9661 aaacgcatgg gtgacttggt ccgtaaccgt gaacgtgtgc aggcagagat gaacgatgcc
     9721 attgcccaag tgcaagagcg tgctgctgaa gaaattgctc ctttggatat tgagttgcaa
     9781 gagttggaag ccagcatttt gatgtattgc accaccaacc gcgatgtttt aactgatggt
     9841 ggcaaggtga agtttgccga tttgattaca ggcaaggtgc aatggcgcaa caacccgccc
     9901 aaggtgcaaa tccgtggcaa cgatgcggtc ttggccttgt tggaacagga tgaaaagtat
     9961 gagcgctttg tacgtaccaa acgcgaagtg aataaagaag cggttttgaa tgaaccagac
    10021 ttttttgcag gcaacccagt gccaggctta agcattgtac aaggcaaaga gttttttgtg
    10081 attgagccat acaaccagca attgcctgaa gcataaaccc aaacccgtgc ggcgcggtac
    10141 gccgctttta aaccactttt aaataggatt taaaccatga ataaaactga atttattaaa
    10201 gcaatggcaa caaacagtgg catgacacaa ggcgatgca
//
LOCUS       DAOMVK010000018        10109 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_1634_length_10109_cov_3.442013, whole genome shotgun sequence.
ACCESSION   DAOMVK010000018 DAOMVK010000000
VERSION     DAOMVK010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 10109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10109
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1634_length_10109_cov_3.442013"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            351..1880
                     /locus_tag="PK856_01385"
     CDS             351..1880
                     /locus_tag="PK856_01385"
                     /inference="COORDINATES: protein motif:HMM:NF017470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HRL01054.1"
                     /translation="MDTYSPSEKLENFIHLGIQAKRQRRYDDAIALYQRALEVDSTYI
                     HTYQAIAKVLFLKGDRQKAIEYYLEYLLRNIGGSDLDIHMLGTEDYRSELIEQYFSTT
                     NHIGHTYVDLDREQNNLLVEYILKQTGFIKKDVVARYLTHFQESYRTVVNGSTDINSE
                     AVISNVKELIIDPDIDVSTVQELAQIYYNKIGQAVSGKFLNWKRIEEMATKDHFINAP
                     SKPVKVINQKPQELNVEQLFELGMTAYASKHLEEHINALKYFHMAAVQEHVMSQYMLG
                     LMYESGIGTEMDVEKSLSWLYKAAKQNLEAAQLAFIRIKYFNKDQQKLLASPDAAMEL
                     TEMHFGANYEHNFEPSRDFKNNPPVVKSNQVGSQTGQKKRIIKAQKQPDGSEEIALWE
                     YAISAVKDNYANFSGRARRREYWGFTLFAYISIIILAILTGAIPIFGIPYFIFCLGIF
                     IPSLSIAVRRLHDVGKSGWFLLLLLIPFVGPIWLLVLNCTDGVVGENEYGPNPKGSLQ
                     A"
     gene            complement(2002..2622)
                     /locus_tag="PK856_01390"
     CDS             complement(2002..2622)
                     /locus_tag="PK856_01390"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRL01055.1"
                     /translation="MNVQRLNSLRHWIFDMDGTLTVPSHDFPYMRRQLGMAEGDLDIL
                     AYVARHNEAEAAQMNQWLWDYEVDLAENAEPATGAADLVQHLHEQERKLAILTRNDKK
                     LALITLQAIGLLHYFDESVILGRDEAAPKPHPAGIDHILQHWQAANDDAIMVGDYEYD
                     LSAGRSAGVHTLLVHPKANHWPELSDYYFEHCGGVLSSLVQGIEIK"
     gene            complement(2763..3266)
                     /locus_tag="PK856_01395"
     CDS             complement(2763..3266)
                     /locus_tag="PK856_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01056.1"
                     /translation="MKKHVFLWVIVGSCLTGAQAESLFPAWRNTAAQFPAATYTGAHA
                     TPKLTAQTRPYRKLFHLMRKGSINYAGEYVLEFASCGADCSMGLIYNARTGATQLLPT
                     GPISSCVDHPNLMPLAETRKEANSRWLQIVATTDHADSPNAPACYTKTFMVERGKIRL
                     IHQQHLY"
     gene            complement(3366..3851)
                     /locus_tag="PK856_01400"
     CDS             complement(3366..3851)
                     /locus_tag="PK856_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01057.1"
                     /translation="MKIWKTVLAASSVLLLMPLAQAAPPSFEQYPAKRYQGKNAAVKL
                     NADTRSFRTRFRDLSHEKANFAGHYAVGYAGCGTGCVLGMMVDVKSGATQFIGSVSDC
                     PPKEGFEGWRDKDIAFKPNSRLLIITGIINEDDNEHPVCSKQYWLEQNGKLKRIRTEV
                     L"
     gene            4313..5335
                     /locus_tag="PK856_01405"
     CDS             4313..5335
                     /locus_tag="PK856_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01058.1"
                     /translation="MDEKVYTELKMKSFLMAISGVLILWVPVLLLHLSIMLFAALITY
                     SASSLIAEKLRKIWPQAKHLPLVGVLSLLVLLALAGYVFGDWLVDKTGSLTITSLLTQ
                     AAVILDQVHTHLPASIAEHVPHSLESLKVSLSHAMKSHAGQVQTAGLHTLRGLGYLIA
                     GFVIGAVAVIQTSGERAAYEKPLSRMVHAEFTELLHGFTDIFYAQIKISAINTVLTAV
                     YLLGILPLIGKPLPMAGTLVMVTFFAGLLPVVGNLISNTFIVILSLSDSLAVTIMSLL
                     WLVGIHKLEYFLNAHIIGHKIKAAMWELLIVMIVLEATFGMAGLVSAPIIYAQAKRSL
                     HNHGWI"
     gene            5522..6103
                     /locus_tag="PK856_01410"
     CDS             5522..6103
                     /locus_tag="PK856_01410"
                     /inference="COORDINATES: protein motif:HMM:NF022995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin domain-containing protein"
                     /protein_id="HRL01059.1"
                     /translation="MSVNNLLEIVDDIKSGMPPETLFSSKDAALFIANKDLLISLGED
                     LVQGFYGIVLSHDKTKAIFKDDEIEMRAEDLKRWWVQSVAGKFDNEYWIWQAYVGVIH
                     VKRGVKNAMMMSMWGWMFDFLSKALFIKTTPENASSLLQAFSRLSATSQALTADTYLE
                     TYLTGILKATGFSKNLLDKMVENEINDLVDAVK"
     gene            complement(6286..6474)
                     /locus_tag="PK856_01415"
     CDS             complement(6286..6474)
                     /locus_tag="PK856_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HRL01060.1"
                     /translation="MRLLIALFLPWLTFFTIGRPFAGLICLILQITLLGWLPATIWAV
                     YALSQYKTDQKIKKALRA"
     gene            6676..7827
                     /locus_tag="PK856_01420"
     CDS             6676..7827
                     /locus_tag="PK856_01420"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HRL01061.1"
                     /translation="MSTILRQLTINQQGLGQTPRFDAGLAGSLQAIEHMGYVQIDTIS
                     VVERAHHHVLWNRVPEYDNSHLNQLVQEQSVFEYWYHAAAYLPMRDYRFALPQMEAVR
                     AGNSRYFSRGDATLMREILARAKDEGSIRLRHVDKKASDQSGGWWNTGPARRSIEQLF
                     MQGDLMVCERNGMEKVYALRENCLPAKLNVTTPNTQECAEYLFETTRRAHGVFTWKQL
                     LHLKTGSTMREAMRAVVNEHLDAGDIVEVMLENGDKAYVEALLMQDFLGETQVKILSP
                     FDNVLIHRERLSALFGFDFRIECYVPAAKRQFGYFCLPILYGDCMVGRIDCKAHRRSK
                     VLQVLSVHWESKTKRSEQLLALLQAELLRFSQFNQCIELDDVILKKAVA"
     gene            8261..8578
                     /locus_tag="PK856_01425"
     CDS             8261..8578
                     /locus_tag="PK856_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01062.1"
                     /translation="MAIIAFDDKSLPKTQTKSNKWLESALLHLEEMVTVLNAKNAVMF
                     ESEGSFIHLATGGVLFKGAKHQQWRFKPFPGERTVQVLQSDELNQQINRSFSAAMAAK
                     KIA"
     gene            complement(8600..9382)
                     /locus_tag="PK856_01430"
     CDS             complement(8600..9382)
                     /locus_tag="PK856_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01063.1"
                     /translation="MSLQRIFGMAFASLGLMAYAQAQDEIPAGVKEECAQLPAVTQQG
                     DKWYRAGQYRKALDAFQKQAGLMSFCLSNIDDGSDDAAKTKRLNVAYNNVGLSHAKLG
                     EYRWARAWYELHPDVAPSPFNLQHLPAIKPSRDKRGQYVRYAGQGQWNYLTVRDGNKG
                     YDIDFEGLRMGLMGWLNGPNMGNFSTSMKKNSQTASYHYEDNGKYQCDISMQFLPANA
                     KGERIEVTQEGDSNCGFGHGVYAGGTYLKISDTVPKERPETY"
     gene            complement(9648..9935)
                     /locus_tag="PK856_01435"
     CDS             complement(9648..9935)
                     /locus_tag="PK856_01435"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRL01064.1"
                     /translation="MQITQTDDGKHGLFRLQDGDNSAGEMKYTWAGPTMFIIDSTHVD
                     DAYRGQNVGRTLLDALVAFAREKNLKVIPLCPYAKAQFDKDASLRDVLREA"
BASE COUNT         2676 a         2023 c         2541 g         2869 t
ORIGIN      
        1 aagactacgc caaacgccgc ggcgtgagct tggaacaagc cgaacgcgac ttggctcctg
       61 tattggggta ttgagggttt gaagtggtaa gtgtgaagtg ataattaaag cggcctgtaa
      121 atgtttacag gccgctttct tattacctga atgacatatt attttgttta aataatagat
      181 atgatatagg ctgaataaat tgcataagtg caacgtttta aataggtata tttaaggttt
      241 tagaattttc taggagacat gtttaagaga atttaaagac ttaaatattc aaaaataggc
      301 atctgaaaat catttttcaa ctaaattaaa taaagtttaa agggaagcgt gtggatactt
      361 actctccgtc agaaaaattg gagaatttta tacatttagg gattcaagca aagcgtcaaa
      421 gacgatatga cgatgctatt gcactgtatc agcgagctct tgaagtagat tcaacttata
      481 tacacactta tcaagcaata gcaaaagtat tgtttttaaa aggtgatcga caaaaggcaa
      541 ttgaatatta tttagaatat ttgcttcgta atataggagg tagtgattta gatattcata
      601 tgcttggcac tgaagattat cgcagtgagc taattgaaca gtatttcagt acaactaatc
      661 atattggtca tacttatgtt gacttggata gggagcagaa taatttattg gtagagtaca
      721 ttttgaagca aactggcttt attaaaaaag atgttgttgc aaggtattta acacattttc
      781 aagagtcata tcggacggtt gttaacggat caacggatat taattccgaa gctgttataa
      841 gtaatgttaa agaattgatt attgatcctg atattgatgt gagtactgta caagagcttg
      901 cgcaaattta ttacaataaa ataggacaag cagtttctgg aaaatttctt aattggaaaa
      961 gaatagagga gatggctact aaggaccatt ttataaatgc tcccagtaaa cctgtaaagg
     1021 taatcaatca aaagccgcaa gagttgaatg ttgagcagct ctttgaactt ggaatgacgg
     1081 cgtatgcatc taagcacttg gaagagcata tcaatgcttt gaaatatttt catatggccg
     1141 cagttcaaga acacgtcatg tctcaatata tgcttggatt gatgtatgaa agtggtattg
     1201 gcacagaaat ggatgttgaa aaatctttat cttggctcta taaagcagcc aagcaaaatt
     1261 tagaagcggc tcaattagcc tttatcagaa ttaaatattt taataaagac caacaaaaac
     1321 ttttggcctc tcctgatgct gccatggagt tgaccgagat gcattttgga gctaattatg
     1381 aacataattt tgaaccaagt cgcgatttta agaataaccc accagtagtc aaaagcaatc
     1441 aagttggctc acaaactggt caaaaaaaga gaattataaa agctcaaaag cagccagatg
     1501 gctctgagga aatagcatta tgggaatacg ccatcagtgc tgtaaaagac aattatgcta
     1561 actttagtgg aagggcgcga agaagagagt attggggatt tactttattt gcttatatta
     1621 gcatcatcat tttggctata ttaacagggg ccatacctat atttggtatt ccttatttta
     1681 ttttttgctt aggaattttt attcctagtt tatctatcgc agtacgaaga ttacatgatg
     1741 ttggtaaatc aggatggttt ttactactgc ttttaatacc atttgttggg cctatttggc
     1801 ttttagtatt aaattgtacg gatggggtag taggtgaaaa tgaatatggg ccaaatccta
     1861 aaggtagttt gcaagcttaa attgtaaatt tctatttatt tggtattgaa atggttttat
     1921 taaaaaccaa taaaaagcgg cctgtaaact tttacaggcc gcttttaaca tggttcaaat
     1981 acattcaaca acaataccac gctattttat ttctatacct tgcaccaagc tgctcaacac
     2041 gccgccgcaa tgttcaaaat aataatcgct gagttctggc cagtggttgg ctttggggtg
     2101 aacgagtaag gtgtgcacgc ctgcgctgcg gcctgcgctg aggtcgtatt cgtagtcgcc
     2161 caccatgatg gcgtcgtcgt tggcggcttg ccagtgttgc aagatgtggt ctatgcctgc
     2221 ggggtggggc tttggagcgg cttcgtcgcg gccgaggatg acgctctcgt caaaataatg
     2281 cagcaagcct atggcttgta aggtaatcag ggcgagcttt ttatcgttgc gggtgaggat
     2341 ggcgagtttg cgctcttgtt cgtgcaggtg ttgcaccaag tctgcggcgc ccgtggcggg
     2401 ctcggcattc tcagccaaat ccacttcgta atcccacagc cattggttca tttgcgcggc
     2461 ttcagcttca ttgtggcggg cgacgtaggc gaggatatcc aaatcgcctt cggccatgcc
     2521 caattggcgg cgcatgtagg ggaaatcatg gctggggacg gtgagggtgc catccatgtc
     2581 gaaaatccag tggcgtaagc tgtttaagcg ttgaacattc atgacggttg tgatggaagt
     2641 gaataataag cctactgtag ggggaatcat gcggcctgta aactctaggt gactttgtga
     2701 ggtgtttaca ggccgcttgt tggcggtgtg ggcatgtcac gaacggcctt tgtgaacgcc
     2761 catcagtaca ggtgttgttg gtggatgagg cgtattttgc cgcgctcgac cataaaggtt
     2821 ttggtgtaac acgcgggcgc gtttgggctg tctgcatggt cggtggtcgc gacaatttgc
     2881 agccaacggc tattggcctc tttgcgggtt tcggccaatg gcatcaagtt gggatggtca
     2941 acacaactgg aaatcgggcc tgtgggcaat aactgggtcg ctcctgtgcg ggcattgtaa
     3001 atcaagccca tgctgcaatc tgcaccgcaa ctggcaaatt caagcacata ttctcccgcg
     3061 taattgatgc tgcctttgcg catgaggtga aacagtttgc ggtaagggcg ggtttgtgcg
     3121 gtgagcttgg gggtagcatg cgcccctgta taagtggcgg ctgggaattg ggcggcggtg
     3181 ttgcgccaag cgggaaacaa ggattcagct tgagcacccg tcaagcagct gcccacaatc
     3241 acccacagga aaacgtgttt tttcatgctg ttagccttgg tgcaaaatag aggtttatgg
     3301 gcatccaatg agtgtgcctc aatgggggag ccaaacattt acaggccgct ttgataagtc
     3361 tgggcttaca aaacttcggt acggatacgt ttgagtttgc cgttttgttc caaccaatac
     3421 tgtttgctgc aaacggggtg ttcgttatcg tcttcgttga tgatgcctgt gatgatgagc
     3481 aagcggctgt tgggtttgaa tgcgatgtct ttgtcgcgcc atccttcaaa tccttctttg
     3541 ggtgggcaat cactgactga gccgatgaac tgggtggcac cgcttttgac gtccaccatc
     3601 atgcccaaga cgcaccctgt gccacagcct gcatagccca ccgcataatg gccggcaaaa
     3661 ttggcttttt cgtggctcaa gtcacgaaag cgggtgcgaa agctgcgtgt gtcagcattg
     3721 agtttgaccg ccgcattttt gccttgatag cgcttggcag ggtattgctc gaagctaggc
     3781 ggtgctgcct gtgccaacgg catcagcagc aaaacgctgc tggctgctaa gactgtcttc
     3841 catattttca tctgacatct cctgctaggt gggtgggaca caatgaagtg ggtttacagg
     3901 ccgcttgttg tgatggcgct cgtttgagtg aacgcttggt tttagcatca taaacagaaa
     3961 atggattagg gtgtgtaaac ctactgtgga atttgagtgc tttttgctta gcagcagagc
     4021 agggctcatg aaccgagttt gccatttaca gatagcatta aaaaattcca ctcgctagcg
     4081 tttatggatg gtggcgtatt gcagctgtgg tgttgccttg atattgatga gtccgcgtca
     4141 tgaactggaa tgtcgtgtcc aaagtaagcc caacgatggg ccagtttgaa tgggtgatta
     4201 acttgctcaa caactgcata tgtgcggcca attctggtga aattattctc ttttcttggg
     4261 tgaaaatagg catagaatat tgttgccact cgatttgggt ggtgtaacag agatggatga
     4321 aaaggtgtat acagaattga aaatgaaatc gttcttgatg gcgatttcgg gggtgttaat
     4381 cttgtgggtg cctgtgctgt tattgcattt gagcatcatg ttgtttgcag ctttgattac
     4441 ttacagcgcc agtagcctga ttgcggaaaa attgcgcaaa atatggccac aagccaagca
     4501 tttgcctttg gtgggggtgt tgtctttgtt ggtcttgctt gccttggcgg gctatgtgtt
     4561 tggggattgg ttggtagaca aaacgggcag cctcaccatc accagcttgc tcacacaagc
     4621 ggcagtgata ctcgatcaag tgcacacgca tctgcctgcg agcattgctg aacatgtgcc
     4681 acattccttg gaaagcctga aagtgagcct ctctcatgcg atgaaaagcc atgcgggaca
     4741 ggtgcaaacg gccgggctgc acaccttgcg cggcttggga tatttgattg cgggttttgt
     4801 gattggggcc gtggcggtga tacaaaccag tggtgagcgg gcggcttatg aaaagccatt
     4861 gagccgcatg gtgcatgctg aattcaccga gttgctgcac ggatttaccg atatttttta
     4921 tgcccaaatc aaaatttctg ccatcaatac cgtgttaacc gcggtgtatt tgttgggcat
     4981 cttgccttta atcggcaagc ctttgcccat ggcaggtact ttggtaatgg tcacgttttt
     5041 tgcaggcttg ttgcctgtgg tgggcaattt aatttccaat accttcatcg tgattttgag
     5101 tttgagcgac agcttggcgg tgaccattat gtcgttgctg tggttggtgg gcatacacaa
     5161 attggagtat tttttaaacg ctcacatcat cggccacaaa atcaaagcgg cgatgtggga
     5221 gttgctcatc gtgatgattg tattggaggc aacatttggc atggcaggat tggtgagcgc
     5281 gcccattatc tatgcccaag ccaaacgcag tttgcacaat cacggttgga tttgaagatg
     5341 aagtgcccat tacataccac ttcaaggttt gaaatcgatg gggtattgag ctgggtttaa
     5401 catttgatat gcagtatttg atgcaaatca aataaattcc atataggttt ttttgatctt
     5461 gaattgggca ttggttctgc tgaaacgagt ctacttactc atttttcagg gaaccatgcc
     5521 catgtctgtg aacaatcttt tagaaatcgt tgatgatatt aaatcgggaa tgccgcctga
     5581 aacactgttt tccagtaagg atgcggcgct gttcatcgcc aataaagatt tgttgatttc
     5641 gcttggcgaa gatttggtac aagggtttta tggcatagtg ctgtcgcatg ataaaaccaa
     5701 agccattttc aaagacgatg aaattgaaat gcgggcagaa gatttgaaac gctggtgggt
     5761 gcaatcggtg gcgggcaaat ttgacaatga gtattggatt tggcaggcgt atgtaggcgt
     5821 gattcacgtg aaacgtgggg tgaaaaacgc gatgatgatg tcgatgtggg gttggatgtt
     5881 tgatttttta tccaaagcct tattcatcaa aaccacgcct gaaaatgcct ccagtttgtt
     5941 gcaagcattc agccgtctct cagccacctc gcaagcattg actgccgaca cctatttgga
     6001 aacctattta acgggtattt taaaagccac gggtttttct aaaaatctgt tggacaaaat
     6061 ggtggaaaac gaaatcaatg atttggttga cgcagtgaaa tagacaccac atgcatgtga
     6121 ttcaaacaat gttttgatat tgcgaatgat ggcagcggaa cattgtcttc acttactacg
     6181 tgtactacgt gcttgtgttg aaataaacag cggctgctgc catacaaaag gcctgtgatt
     6241 gtgaataaca gttacaggcc gctttattat ctcagatact ctgaatcaag ccctcaaggc
     6301 ttttttaatt ttctggtcgg ttttgtattg tgacaaagca tacactgccc aaatggttgc
     6361 aggcagccaa cccaataggg taatttgcaa aatcaaacaa atcagccccg caaacggcct
     6421 gcctatggta aaaaacgtca accatggtaa gaacaatgca atcagtaatc tcattctacg
     6481 cctttcatta agttattatt ctagcgacat catagcctaa ctgagcttat gtcaccaagc
     6541 atggtaggac aacagtcgtg gatagcatgg ctaatgattg aataaagccc gtaaaatgaa
     6601 cgcagcccgc caccaatgcg gtgatttcaa tttggtattt ttttcaaact agcagacgtt
     6661 ttcgactgag aatttatgag cacaatattg cgccaactga ccatcaacca acaaggattg
     6721 ggccaaactc cacgttttga tgctggcttg gctggtagtt tgcaagcgat tgaacacatg
     6781 ggttatgtgc aaatcgacac catttccgtg gtagagcggg cacatcatca tgtgttgtgg
     6841 aatcgagtgc ctgaatacga taacagccat ttgaatcaat tggtacaaga gcaaagcgtg
     6901 tttgaatatt ggtatcacgc agccgcttat ttgcctatgc gcgactaccg ctttgcattg
     6961 ccacaaatgg aggcggtgcg tgcaggcaac agccgatact tcagtcgagg cgatgccact
     7021 ttgatgcgag aaatcttggc cagagccaaa gatgagggca gcatacgttt gcgacatgtg
     7081 gataaaaaag ccagcgatca gagcggtggc tggtggaata cagggcctgc acgccgatct
     7141 attgaacaat tgttcatgca aggtgatttg atggtgtgtg agcgcaatgg catggagaaa
     7201 gtctatgctc tgcgcgaaaa ctgtttgccc gccaaattaa atgtgaccac accaaacaca
     7261 caagagtgtg cggaatattt gtttgaaact acgcgcagag cacatggcgt gttcacttgg
     7321 aagcaattgc tgcatttaaa aacaggcagc accatgcgag aggccatgcg tgccgtggta
     7381 aacgagcatt tggatgcagg tgacattgtt gaggtcatgc ttgagaacgg cgataaggca
     7441 tatgtggagg ccttactgat gcaagacttc ttgggcgaaa cccaagtgaa aattctttct
     7501 ccattcgaca atgtgctgat acacagagag cggttgagtg ctttgtttgg gtttgatttt
     7561 cgcattgaat gttacgtgcc tgcggcgaaa cggcagtttg gctatttttg cttgcccatc
     7621 ttgtatggtg attgcatggt ggggcgcatt gattgcaagg cgcacaggcg cagcaaagtt
     7681 ttacaagtgt tgagtgtgca ttgggaatcc aaaaccaagc gatcagaaca attgttggca
     7741 ttgttgcagg cagagttatt gaggtttagc cagtttaatc aatgtatcga attggacgat
     7801 gttattttga aaaaagccgt ggcttgagtt gtgccacgat tcattgcgtc atgaatgaat
     7861 agccagtcaa taaatcggtg tctgaaatga acagtataga ttttcaagca ataggcctat
     7921 tgaaatttga atggaaattt caaagcaaaa aacagtttac aggccgctat tgcagagagt
     7981 atgcaaaaaa acaaatatgc gtgtgtaaga tgtaaattgg tatttaagtc tgatattggc
     8041 aaatacgtga gaattttaag gcttagtctc ggcattgctt gtgatgtgtc aaaagataat
     8101 gctgcggttg gcgctagact gtattcattg ttttcaccgt tcattcagct ttggtgtgaa
     8161 ccaaattttg ccataacata agttttcaat tttggcatta aatttggtat ggtttatgct
     8221 gtatggatgc tcattattca cttcacattt cagtattgcc atggctatca ttgcttttga
     8281 cgacaaaagt ctgcccaaaa cccaaaccaa atccaacaaa tggttggaat cggctttgtt
     8341 gcatttggaa gaaatggtca cggtgttgaa tgcaaaaaat gcggtgatgt ttgaatcaga
     8401 aggcagcttc atacacttgg ccaccggtgg cgtgttgttc aaaggcgcga aacaccaaca
     8461 atggcgtttt aaaccttttc ctggtgaacg cactgtgcaa gtattgcaaa gcgatgaact
     8521 caaccaacaa atcaaccgca gtttcagtgc ggccatggcg gcgaagaaaa tcgcctaatc
     8581 ctaggcgcac catgatcttt taatacgttt caggccgctc tttgggcacg gtgtcgctga
     8641 ttttcaagta cgtgccaccc gcatacaccc catgaccaaa accacagttg ctgtcacctt
     8701 cttgagtcac ttctatgcgt tcacctttgg cattggcagg taaaaactgc atgctgatgt
     8761 cacattggta tttcccattg tcttcataat ggtagcttgc cgtttggctg tttttcttca
     8821 tgctggtgct gaaattgccc atattggggc cgttgagcca gcccatcaaa cccatgcgca
     8881 aaccctcaaa atcaatgtcg taacccttgt tgccatcgcg cacggtgagg taattccatt
     8941 gcccttgccc tgcatagcgc acgtattggc cacgcttgtc acggctgggc ttgatggcag
     9001 gcaagtgttg caagttgaat gggctgggtg cgacgtcggg atgcaattcg taccaagcac
     9061 gtgcccagcg gtattcgccc agtttggcat ggcttaagcc gacgttgttg taggcgacat
     9121 ttaggcgttt ggttttggcg gcatcatcgc tgccatcgtc tatattgctc aaacaaaacg
     9181 acatcaatcc cgcttgtttt tgaaatgcat caagcgcctt acggtactgg cccgcacgat
     9241 accatttgtc tccttgttgg gtgaccgcag gcagctgcgc gcactcctct ttaacacctg
     9301 caggtatctc gtcttgcgcc tgcgcataag ccatcaaacc caagctggca aatgccattc
     9361 caaaaatgcg ttgcaaagac atggtgtgct ccttaagaaa atgaaaccca atcagataag
     9421 tgaggtgttt gtggaggcat tatttttgat tgcagtgggc atgtaaatcg cgcggaacaa
     9481 atgcacaaaa aatgccactg caagcggcag aatacaaact gatcgatgca agcaagatgg
     9541 cttaatagca atttgtctgt ttacaggccg catgatgtgt tgcaaacagg tatgcaaacc
     9601 cacacatgcg ttcaccagcg gcctgtaaca tggttacagt ccttgcgtta agcctcgcgc
     9661 aacacgtcgc gcaagctggc gtctttgtca aactgcgctt ttgcataagg gcacaaagga
     9721 atgactttga ggtttttctc acgcgcgaat gccaccaagg catccaataa ggtgcggccc
     9781 acattttgac cacggtaagc atcgtccaca tgggtggaat cgatgatgaa catggtcggg
     9841 cctgcccaag tgtatttcat ttctccggcg ctgttgtcgc catcttgtaa gcgaaacaag
     9901 ccgtgtttgc catcgtcggt ttgggtgatt tgcatgagtg tctccatgag aaagctaaag
     9961 ggtttagagc aagagccatc ttagcaccag ttgcactgga tgaggcaatt catccaaagg
    10021 agcgatgcta tactcatgcc atccgatttt tgaattgtga agcgccatgt ctgaatccca
    10081 actgagccgt gaaatttctg ttgcccgtc
//
LOCUS       DAOMVK010000019        10062 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_1654_length_10062_cov_2.887079, whole genome shotgun sequence.
ACCESSION   DAOMVK010000019 DAOMVK010000000
VERSION     DAOMVK010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 10062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10062
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1654_length_10062_cov_2.887079"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3016)
                     /gene="mfd"
                     /locus_tag="PK856_01440"
     CDS             complement(<1..3016)
                     /gene="mfd"
                     /locus_tag="PK856_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRL01065.1"
                     /translation="MSGGLFTIPTAGQKSVWSELSLGSAPWQLATHLPNKRPVMVLTE
                     DVDQALRIQEALSFYQPELKTAFFPDWETLAYERFSPHQDLVSERLAVLWQLKNQQID
                     MVLLPVATAMQKLAPLSFIQGRTFWLKVGEALNDEQFKAQLVDAGYNHVSNVVQAGEF
                     TVRGGLLDVFPMGSEWPYRIDLFGDEIDSIKTFDPDTQRTITPVSEIRLLPAHEFPTD
                     ETTQELFRSQFREHINGDPTRALVYQAVSKGQFGSGVEYYLPLFFEQPLATVFDYLPE
                     NTLLVSLQDALAVAHTHWLDVKSRYEMAGADPSYPPLPPQALFLSEDELGAHLKPLAH
                     IVLRPQSHDFDLPVVAVDRSKEQPLQALQQFQATFNGKILLCAESLGRRETMLAFLQQ
                     QQLGVQTVASWQDFLHSEAGLNLAVAPVARGFQATGEKLAVISEADLYQHVVRSGRRQ
                     KRRKHADVSDSGLRDLAEINTGDPVVHEEHGIGRYMGLVSLDLGEGDNEMMLLEYADG
                     GQLYVPVAQLHLISRYAGHAADNVHLHKLGNASWGKAKRKAAEKARDTAAELLNLYAQ
                     RAALEGHAFGLNEQDYQQFADGFGYEETEDQASAIVATIKDLLAGKPMDRLICGDVGF
                     GKTEVALRAAFVAVMGGKQVAVLAPTTLLVEQHAQNFADRFADFPIKVAQLSRFNSSK
                     ETKAALAGLQDGTVDIVIGTHKLVQPDIEFNNLGLVIIDEEHRFGVRQKEQLKRLRAN
                     VDVLTMTATPIPRTLSMALEGLRDFSLITTAPSRRLAVKTFVKPFSEATVREAIVREL
                     KRGGQVYFLHNEVDTIENVFERLSELVPEAKIAIAHGQLRERELEQVMRDFMQHRYNV
                     LLCSTIIETGIDIPNANTIIINRADKFGLAQLHQLRGRVGRSHHQAYAYLLAPEYITK
                     DAEKRLDAIAAADELGSGFTLAMQDLEIRGAGEILGEGQSGEILQVGLTLYSEMLRQA
                     VRDLKKGRVPDLDAPLGVTTEIKLHSPALLPEG"
     gene            3200..3754
                     /locus_tag="PK856_01445"
     CDS             3200..3754
                     /locus_tag="PK856_01445"
                     /inference="COORDINATES: protein motif:HMM:NF022995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin domain-containing protein"
                     /protein_id="HRL01066.1"
                     /translation="MSLNINTANDADLAEFLSHNGFCEADANCLQALHPHISPLLPAL
                     TEAFYERILSSDAMRPFVIDRVDSLKQTHLRWMNMLFSGPFDDQFVQIHQHIGEVHLK
                     QKIPPLFVASSMAFLRAEMPKLFTPELLAKFPQYDYAFCTSALLRLIDTCQFLVDRTY
                     YQSLMELLGISPKLFLRLMTSSSK"
     gene            complement(3799..4383)
                     /gene="metW"
                     /locus_tag="PK856_01450"
     CDS             complement(3799..4383)
                     /gene="metW"
                     /locus_tag="PK856_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HRL01067.1"
                     /translation="MRELREDLRLIYDWIAPNSKVLDLGCGDGQLLAALVEEKNCIAY
                     GVELDTDSVIAAMDLGVNVIQSDLESGLRHGFADNSFDVVVLSQTIQAMKNTEIILQE
                     MKRVARQAIVTFPNFGYWRNRLQIAQGRMPVSESMPYEWYNTPNIHWCTLADFDALCA
                     KNGLAVLERTVMSGGKRIDFLPNLLGSLAFYRVG"
     gene            complement(4383..5522)
                     /locus_tag="PK856_01455"
     CDS             complement(4383..5522)
                     /locus_tag="PK856_01455"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HRL01068.1"
                     /translation="MTNNSGSVGVVTPQKIAFEQPLALQNGQVLPRFDLMVETYGVLN
                     AAKSNAVLICHALSGNHHVAGKHAESDKHSGWWDNMVGPGKPIDTNAFFVIALNNLGG
                     CHGSSGPLSLNPETGKTYGADFPMVTVKDWVQTQARLADYFGIDKWAAVIGGSLGGMQ
                     TMQWAIDLPERVRHAVIIASAPKLSTQNIAFNDVARQSIITDPDFHAGHYQEHQTLPR
                     RGLRIARMMGHITYLSEDGLGKKFGRALRNDDYQFNYDIDFEVESYLRYQGDKFAEIF
                     DANTYLLMTKALDYFDPAAAFNDDLQAAFKHITAKCFVVSFSTDWRFSPERSHEIVRA
                     LIAAEKPVQYLEVLSQHGHDAFLMEDAAYMRGMHAYMGKIAKEVA"
     gene            5788..6621
                     /locus_tag="PK856_01460"
     CDS             5788..6621
                     /locus_tag="PK856_01460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRL01069.1"
                     /translation="MITDQIPNKPTLLSAALIVKNEAAKLEACLQSLVGWVDEIVILD
                     SGSTDATLDIAKKYGAAVYSHTDWQGFGPQRQRAQSHVQGEWVMWLDADERVTPELKQ
                     SLQAALKQYQHDTNVIFEFNRYSWAFGKFIRHCGWYPDRVLRVYRPAYTGYNNALVHE
                     SVVKPHDANVINLRGDLLHYTFDSLPHYMTKQTQYASAWAEQKQQHGKKVSYGSALSH
                     AAFTFVKMYLLKRGFLDGKQGLLLSVLSAQFVYVKYMDLWLKQHTQSAQDYEAEHNSH
                     Q"
     gene            complement(6610..6984)
                     /locus_tag="PK856_01465"
     CDS             complement(6610..6984)
                     /locus_tag="PK856_01465"
                     /inference="COORDINATES: protein motif:HMM:NF016161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HRL01070.1"
                     /translation="MTYLHLKYMHMTLVLVSVLFFNLRFFMRMGKPSDSLPKWFKILP
                     HVNDTFLLVSGLTLVGYSGWVPFGNAPWLGWKLVLLVVYVLLGHKAMRTIPERQAWLW
                     WALGMACVTAMAVLALYKPLLV"
     gene            7248..>7792
                     /locus_tag="PK856_01470"
     CDS             7248..>7792
                     /locus_tag="PK856_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HRL01071.1"
                     /translation="MSALTLYDKLWNSHIVNEADDGTVLLYIDRHLVHEVTSPQAFEG
                     LKLNERPLWRKDSIVSTADHNTPTDHWDEGIKDPISKLQVDTLDANIKEFGALAYFPF
                     KDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIEVNGELATGITA"
     assembly_gap    7793..7802
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7803..8650
                     /locus_tag="PK856_01475"
     CDS             <7803..8650
                     /locus_tag="PK856_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HRL01072.1"
                     /translation="IIGQIGTAGGTGYAIEFGGSAIRSLSMEGRMTLCNMAIEGGARS
                     GLVAVDQTTIDYVKGRPFAPKAEQWEQAVTYWKTLVSDEGAQFDKVYRFDAADIQPQV
                     TWGTSPEMVLSVDGTVPNPADEQDHVKREGIERALQYMGLQAGTALTDIQIDSVFIGS
                     CTNSRIEDLREAAAVTKGHKKADNVKRVLIVPGSGLVKEQAEQEGLDQVFIEAGFEWR
                     EPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGNGGRTHLVSPAMAAAAAIKGHFVD
                     VRSFL"
     gene            8796..8921
                     /locus_tag="PK856_01480"
     CDS             8796..8921
                     /locus_tag="PK856_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003791058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HRL01073.1"
                     /translation="MKKLTLLVCAAVLVLAGCNTIKGAGQDISSGGQAVSNAASQ"
     gene            8957..9097
                     /locus_tag="PK856_01485"
     CDS             8957..9097
                     /locus_tag="PK856_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HRL01074.1"
                     /translation="MKRLVLIACAAVVFLAGCNTFKGAGEDISRGGQAVSNTATDVQQ
                     RM"
     gene            9164..9805
                     /gene="leuD"
                     /locus_tag="PK856_01490"
     CDS             9164..9805
                     /gene="leuD"
                     /locus_tag="PK856_01490"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRL01075.1"
                     /translation="MKAFTTINAVVAPLDRANVDTDAIIPKQFLKSIKRSGFGPNCFD
                     EWRYLDHGEPGMDNSQRPLNPDFVLNQARYASVNILLTRKNFGCGSSREHAPWALDDY
                     GFRAVIAPSFADIFFNNCYKNGLLPIVLPEEVVDQLFQEVEANEDYQLTVDLPNQTVT
                     TPSGQSFGFEITEHRKHCLLNGLDEIGLTLAHAEEIKAFETKHRNAQPWLFQD"
     gene            9899..>10062
                     /locus_tag="PK856_01495"
     CDS             9899..>10062
                     /locus_tag="PK856_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRL01076.1"
                     /translation="MTQTIAVLPGDGIGPEIIAQAVRVLEVFKARGFDVQFQFAPLGG
                     AAYDEHGAPYP"
BASE COUNT         2655 a         2640 c         2284 g         2473 t
ORIGIN      
        1 agccttcagg caataaggca ggactgtgca atttgatttc cgtggtcacg cccaaaggtg
       61 cgtccaaatc gggcacacgt ccttttttca aatcgcgcac cgcttgtctg agcatttccg
      121 aatacagggt caagcccact tgcaagattt cccccgattg gccttcgccc aaaatctcgc
      181 ccgcaccgcg aatttccaag tcttgcatcg ccaaagtaaa gcctgagccc aactcatcgg
      241 cggcggcaat cgcatcgaga cgtttttccg cgtctttggt gatgtattcg ggcgcaagca
      301 aataggcata ggcttggtga tgcgaacgcc cgacacggcc acgcaattgg tgcaattggg
      361 ccaagccaaa tttgtcggcg cggttgatga tgatggtatt ggcattgggg atgtctatgc
      421 ctgtttcgat aatggtagag cacagcaaca cattgtaacg gtgttgcatg aagtcgcgca
      481 tcacttgttc caattcgcgt tcgcgcaatt ggccgtgggc aatggcaatt ttggcctcgg
      541 gcaccaactc actcaagcgc tcaaacacgt tttctatggt atcgacctca ttgtgcaaga
      601 aatacacttg gccaccacgc ttgagctcgc gcacaatcgc ttcgcgcacc gtggcttctg
      661 aaaatggctt cacaaacgtt tttaccgcca aacggcgact cggtgcggtg gtaatcaatg
      721 aaaaatcgcg caagccttcc aaagccatgg acagcgtgcg cggtattggc gtggcggtca
      781 tggtgagcac gtccacattg gcgcgcaaac gcttgagctg ctctttttgg cgcacgccaa
      841 agcggtgttc ctcatcaatg atgaccaaac ccagattatt gaattcaatg tcgggctgca
      901 ccaatttgtg cgtgcctatt acaatgtcta ccgtgccgtc ctgtaaacct gccagagcgg
      961 ctttggtttc cttgctggaa ttgaagcgcg acaattgcgc cactttaatc ggaaagtcag
     1021 caaaacggtc ggcaaagttt tgcgcgtgtt gctccaccaa cagcgtggtt ggcgccaaca
     1081 ccgccacttg cttgccaccc attaccgcca caaaagcagc gcgcagcgcc acttcggttt
     1141 tgccaaagcc cacatcgcca caaatcaacc tgtccatagg cttgcctgcg agcaagtctt
     1201 tgatggtggc gacaatggca ctggcttggt cttcggtttc ttcatagcca aagccatcgg
     1261 caaattgctg atagtcttgc tcatttaagc cgaaggcatg gccttcaagc gcggcgcgtt
     1321 gggcatacag attgagcaat tcggcggcgg tatcgcgcgc tttttcggcg gctttacgct
     1381 tggccttgcc ccatgaggca ttgcccaatt tgtgcaaatg cacattatcg gccgcatggc
     1441 ctgcatagcg cgaaatcaaa tgcaattgcg ccacgggcac gtacaattgc ccgccatcgg
     1501 cgtattcaag caacatcatt tcgttgtcgc cctcgcccaa atccaagctg accaagccca
     1561 tgtagcggcc tatgccatgc tcttcatgca ccacagggtc acctgtgttg atttcagcca
     1621 aatcgcgcaa gccgctgtct gacacatctg cgtgtttgcg gcgtttttgg cggcgaccag
     1681 aacgcaccac gtgttggtat aaatcggctt cactgatgac ggccagtttt tcgcctgtcg
     1741 cttgaaaacc acgcgccaca ggcgctaccg ccaaattcaa tcccgcttcg ctgtgcaaaa
     1801 aatcttgcca cgaggccacg gtttgcacgc ccaattgctg ttgctgcaag aaagccaaca
     1861 tggtttcacg tcgccccaag ctctcggcac acaacagtat tttgccattg aaagtggcct
     1921 gaaactgttg caaggcttgt aaaggctgct ctttactgcg gtctactgcc accacgggca
     1981 aatcgaaatc atgactttgc ggacgcaaca caatgtgggc aagcggcttt aagtgtgcgc
     2041 ccagttcgtc ttcactgaga aacaaggctt gcggtggcag cggcggatag cttgggtctg
     2101 cgccagccat ctcatagcgc gacttcacat ccagccaatg ggtgtgcgcc acagccaaag
     2161 catcctgtaa actcaccaac agtgtgtttt caggcaaata atcaaaaaca gtcgccaaag
     2221 gctgctcgaa aaacagcggc aaataatact ctacgccact gccaaactga cctttgctga
     2281 cggcttggta taccagcgca cgggttgggt caccattgat gtgttcgcga aactggctgc
     2341 gaaacaattc ttgcgtggtt tcatcggttg ggaactcatg cgcgggcaag aggcggattt
     2401 cagacacagg tgtaatcgtg cgttgggtat cggggtcgaa agttttgatg ctgtcgattt
     2461 catcgccaaa caaatcaata cggtatggcc actcagaacc cataggaaac acatccagca
     2521 aaccgccacg cacggtaaat tcaccggctt gcaccacatt ggaaacgtgg ttatagcctg
     2581 catcgaccaa ctgcgctttg aattgctcat cgttgagtgc ttcgcccact ttgagccaaa
     2641 aggtgcgccc ttgtatgaaa gacagtggcg ccaatttttg catggcggtc gccacaggca
     2701 gcaaaaccat atcaatttgc tgatttttca attgccacaa cactgccaaa cgctcagaca
     2761 ccaaatcttg gtgcggtgaa aaacgctcat acgccaaagt ttcccaatca ggaaaaaacg
     2821 cggttttcag ctcgggttgg taaaacgaca aagcctcttg tatgcgcagt gcttggtcta
     2881 cgtcttcggt taaaaccatt acaggtcgct tgtttggcag gtgtgtggcc aattgccaag
     2941 gcgcgctgcc caaggacaat tcagaccaaa cggatttttg acctgctgtg ggaattgtaa
     3001 ataaaccacc agacatactg tttaccttaa gtttgatgcc catcaaatag ctgggattat
     3061 ccgattgtaa caaaagactt atttttgttt atatattaaa atgctgtgtt tcaccttatc
     3121 catatcacat attacatgcc caatatggcg gtcgaaaaca tcacacacac ttgttttatt
     3181 agtatcgaaa gcacacatca tgagtttaaa catcaacacg gccaatgatg cagatttagc
     3241 cgaatttttg agtcacaatg gcttttgcga agcagatgcc aactgtttac aagcactgca
     3301 tcctcacatc agcccattgc tgcccgcctt aaccgaggcg ttttatgagc gcattttgtc
     3361 atctgatgcc atgcgcccat ttgtcatcga ccgtgtagac agcctgaaac aaacacactt
     3421 gcgctggatg aacatgttgt tcagcggtcc atttgacgac cagtttgttc aaatacacca
     3481 acacattggt gaagtgcatt tgaaacaaaa aattccaccg ttatttgtgg catcaagcat
     3541 ggcttttttg cgcgctgaaa tgcccaaatt gttcacgcct gaattgttgg cgaagtttcc
     3601 acaatacgat tatgccttct gcacctcagc cttgttacgc ttaatcgaca cttgccaatt
     3661 cttggtagac cgtacttatt accaatcttt gatggaattg ttgggcattt cacccaaatt
     3721 gttcttgcgc ttgatgactt cctcaagcaa ataaaaagcc aaatcaaaat gccccgcgtt
     3781 tgcggggttt ttcatgcctt aaccaactcg gtaaaacgcc aagctgccca ataaattagg
     3841 caaaaagtct atgcgtttgc cacccgacat caccgtgcgc tccaacactg ccaagccatt
     3901 tttggcacac aaagcatcaa aatccgccaa ggtacaccaa tggatgttgg gcgtgttata
     3961 ccactcatac ggcatggatt cagacacagg catgcgccct tgggcaattt gcaaacggtt
     4021 gcgccaatag ccaaaattcg ggaaggtcac aatggcttgt ctggccaccc gcttcatttc
     4081 ttgcaaaatg atttcggtgt ttttcatcgc ctgtatggtt tgggacaaca ccaccacatc
     4141 aaaactgttg tctgcaaagc catggcgtaa tccgctttcc aaatcagact gaatcacgtt
     4201 cacgcccaaa tccatcgctg caatcacact gtcggtgtcc agctccacac cataggctat
     4261 gcaatttttc tcttcaacca aggcagccaa caattgaccg tcgccgcagc ccaaatccaa
     4321 caccttagaa ttgggtgcaa tccagtcata aatcaagcgc aagtcttctc gcaattcacg
     4381 catcaggcca cctctttcgc aattttgccc atgtaggcat gcatgccacg catgtaagca
     4441 gcatcttcca ttaaaaacgc atcatggcca tgttgcgaca acacttccaa atactgcaca
     4501 ggtttttctg ccgcaatcaa agcgcgcacg atttcgtgcg aacgctctgg gctgaaacgc
     4561 caatcggtac tgaacgacac cacaaaacat ttggccgtaa tgtgtttgaa agcggcctgt
     4621 aaatcatcgt taaacgcggc ggcagggtca aaataatcca aagccttggt catcaacaaa
     4681 taggtattcg catcgaagat ttccgcaaat ttatcgcctt ggtagcgcag atatgactcc
     4741 acttcaaaat caatgtcgta attgaattgg taatcatcgt tgcgcagcgc acggccaaac
     4801 tttttgccca agccatcttc cgacaaatac gtgatgtgac ccatcatgcg ggcaatgcgc
     4861 aagccgcggc gtggcaaagt ctggtgctct tgataatggc cggcatgaaa atcaggatcg
     4921 gtaatgatgg attgacgcgc cacatcatta aacgcgatgt tttgtgtcga caatttgggc
     4981 gcagaggcaa tgatgacagc gtggcgcaca cgttcaggca ggtctattgc ccactgcatg
     5041 gtttgcatac cgcccaaact accccctatc actgccgccc acttgtctat gccaaagtaa
     5101 tcggccaaac gcgcttgcgt ttgcacccaa tctttgacgg tgaccatggg gaaatcggca
     5161 ccataagtct tgcctgtttc tggattgagg ctcaaaggcc cactgctgcc atgacagccg
     5221 cccaaattgt tcaacgcaat cacaaaaaac gcattggtat cgatgggttt gccaggacca
     5281 accatattgt cccaccagcc gctgtgttta tcgctttcag catgcttgcc tgccacatgg
     5341 tggttgcccg acaaggcatg gcaaatcaac acggcattgc ttttggcggc attcaaaaca
     5401 ccataagtct ctaccatcaa atcaaatcgg ggcaatactt ggccattttg caatgccaac
     5461 ggctgttcaa atgcaatttt ttgtggcgtg accacaccaa cactgccaga attattcgtc
     5521 atcgtgtcaa cacaagttag cgcatctgcg caaatgatgt cattataatg gttttggcat
     5581 gattggctag gtggaacggg tgcagatact gacaaaatta ttgcacattg cagcatggat
     5641 tttcgctgcc attgccttaa tcagcctgtt cctgaatcgc aatcagcgtt caatcatcca
     5701 caaacatgtg cgcctcgttg ccattgtttt gctcaccatc agtgctggca cacttttgct
     5761 gcatttattg acatagttaa taccatcatg atcactgacc aaatccctaa caaacccacc
     5821 ctattaagtg ctgctttaat cgttaaaaac gaagccgcca aactggaggc ttgcttgcaa
     5881 agcttggttg gctgggtgga tgagattgtg attcttgatt ctggcagcac cgatgccact
     5941 ttggacatcg caaaaaaata cggtgctgca gtctattccc acaccgattg gcaagggttt
     6001 ggcccgcagc gccagcgagc gcaaagccat gtgcaaggtg aatgggtgat gtggttggat
     6061 gccgatgagc gggtcacccc tgagttaaaa cagtctttac aggccgcatt aaaacaatac
     6121 cagcatgaca ccaatgtgat ttttgaattc aaccgctaca gctgggcatt tggcaagttt
     6181 attcgccact gtggttggta tcccgaccgc gtgttgcgcg tgtaccgccc tgcttacaca
     6241 ggctacaaca acgccttggt gcacgaatcc gtggtcaagc cacatgatgc caacgtgata
     6301 aacctgcgtg gcgacttgct acactacaca ttcgacagtt tgcctcacta catgaccaag
     6361 caaacccaat acgccagcgc ttgggctgag caaaaacaac aacatggaaa aaaagtgagc
     6421 tatggcagcg ccttaagtca cgctgcattt acctttgtta agatgtatct tcttaagcgc
     6481 ggttttcttg atggcaaaca aggcttgctc ttgagtgtgc tgtctgcaca gtttgtctat
     6541 gtgaaataca tggacttgtg gctcaagcaa catacccaat cggctcaaga ttatgaagcc
     6601 gaacataatt cacaccaata aaggcttgta caaggccaga actgccatcg ctgttacgca
     6661 agccattccc agtgcccacc acaaccatgc ttgccgctct ggaatggttc tcatcgcttt
     6721 gtgccccaat aacacataca ccaccaacaa aaccagcttc caacccaacc acggtgcatt
     6781 gccaaacggc acccatccgc tgtagcccac caaggtcaaa cccgatacca ataagaacgt
     6841 atcattcaca tgcggcaaaa ttttgaacca tttgggcaaa ctgtctgacg gcttgcccat
     6901 gcgcatgaaa aatcgcaggt taaagaacag cacactcacc aagaccaaag tcatgtgcat
     6961 gtatttcaag tgtagataag tcatattata gccacatcaa tatttttact gattataaag
     7021 tgcaaactta cctaaaacct tgatacaaat ctacaagact tcagcaacct tattttgctg
     7081 ccttatttta tacaatttgt ccaaattact catattgtcc aaataaatat tagcaaacgc
     7141 ggcctgcaaa tatttatttt ggacaaattc ttgccaaatg ttgcacagca acctaatatg
     7201 agcgagcacg aacgtctaat aatcagttaa ccaaccggat gcaccgcatg agcgctttaa
     7261 ccttatacga taaattatgg aacagccaca tcgtgaacga agccgacgat ggcacagttc
     7321 tgctttacat agaccgtcac ttggtacatg aagtcaccag cccacaggca tttgagggtt
     7381 tgaagttaaa tgaacgtcca ttgtggcgca aagacagcat tgtcagcaca gccgaccaca
     7441 acacccctac cgaccattgg gatgagggca tcaaagaccc catctccaaa ttgcaagtcg
     7501 atactttgga tgccaacatc aaagaatttg gcgcactggc ttatttccca ttcaaagaca
     7561 aaggccaagg aatagtgcat gtgatgggcc cagagcaagg tgcaactctg ccaggcatga
     7621 cagtggtttg tggcgactca cacacttcta cccatggtgc atttggcgca ttggcacacg
     7681 gcataggcac gtctgaagtg gaacacacca tggccactca atgcattact gctaaaaaat
     7741 ccaaatccat gttgattgaa gtcaacggtg aattggctac aggcatcacg gcnnnnnnnn
     7801 nnacatcatt ggtcaaatcg gcactgctgg cggcacaggc tatgcaattg aattcggcgg
     7861 cagcgccata cgttcactca gcatggaagg ccgcatgact ttgtgcaaca tggccattga
     7921 aggcggcgcc cgctctggct tagtagctgt agaccaaacc accatcgatt atgtgaaagg
     7981 ccgtcctttt gcgcccaagg ctgaacaatg ggaacaagcc gtgacctatt ggaaaacact
     8041 ggtttctgat gaaggtgcac agtttgataa agtttaccgt ttcgatgctg ccgacattca
     8101 acctcaagtc acttggggca catcacctga aatggtcttg tcagtggatg gcaccgtacc
     8161 caatcccgct gatgagcaag accatgtcaa acgcgaaggc atagaacgtg ccttacaata
     8221 catgggccta caggccggca cagccttgac cgacatccaa attgattcgg tattcatagg
     8281 ttcttgtacc aacagccgca ttgaagactt gcgcgaagcc gctgccgtca ccaaaggcca
     8341 caaaaaagct gacaacgtga aacgtgtact catcgtacct ggttcaggct tggtaaaaga
     8401 gcaagcggaa caagaaggct tggaccaagt gtttatcgaa gcaggctttg aatggcgtga
     8461 gccaggttgc tccatgtgct tggcgatgaa tgccgaccgc ttggaaccag gcgagcgctg
     8521 cgcttccact tcaaaccgca actttgaagg ccgtcaaggc aatggtggtc gcacccattt
     8581 ggtcagtcct gccatggctg ccgctgccgc catcaaaggc cattttgttg atgtacgcag
     8641 tttcttgtaa aaattaaacc accaaggaac cattgccgca ctcaggtatc agaaacactg
     8701 ttaattgctt gtcatttaga ggctggcact gacactcagc ttctcatgta tcaagcaaga
     8761 aggctgacat tttgaaacca acaaaaggag ttgaaatgaa aaaattaact ttacttgtgt
     8821 gtgctgcggt attggtattg gcgggttgta acaccatcaa aggtgctgga caagacatca
     8881 gttctggcgg ccaagctgtc agcaatgccg cttcgcaata gttttagact tggacttcca
     8941 tttaaggaga tgtgtcatga aacgattggt cttgattgct tgtgcagcag ttgtgttttt
     9001 agctgggtgc aatacgttta aaggtgcagg cgaggacatt tctcgcggcg ggcaagcggt
     9061 gagcaacacc gctaccgatg tacaacaacg catgtaagct tgttttcagg cggctacagg
     9121 ccgcctgtca ttgcacacac ccagctttta tttggaaacc ctcatgaaag cttttaccac
     9181 catcaatgct gtagtggcac cgctagaccg tgcgaatgtg gacaccgatg ccatcattcc
     9241 aaaacaattc ttgaagtcca tcaaacgctc aggttttggc cccaattgct tcgatgaatg
     9301 gcgctattta gaccatggcg aaccaggcat ggacaacagc caacgcccat taaaccctga
     9361 tttcgtcctc aaccaagccc gttacgcctc tgttaatatt ttgctcacgc gcaaaaactt
     9421 tggctgtggt tccagccgtg aacatgcacc ttgggctttg gatgattatg gcttccgtgc
     9481 cgtgattgcc ccatcttttg ctgatatttt cttcaacaac tgttacaaaa atggcttgtt
     9541 gcccatcgtg ttgcctgaag aagtggttga ccaattgttc caagaagtgg aagccaacga
     9601 agactatcaa cttactgttg atttgcccaa ccaaaccgtg accacgccca gcggtcaaag
     9661 ctttggcttt gaaatcaccg aacaccgcaa gcattgcttg ttaaacggtt tggatgaaat
     9721 aggcctgact ttggcacatg cggaagaaat taaagcattt gaaaccaagc atcgcaacgc
     9781 acaaccttgg cttttccaag attaatcctc taaaataccg ctgaatcttg caggtatgca
     9841 cttacaggac gcttcaagcg tcctgtaaaa cactcgatag caaacaaggc aaacaaacat
     9901 gactcaaacc attgcagttt tacccggcga cggcatcggc ccagaaatca ttgcacaagc
     9961 agtgcgcgta ttggaagtgt tcaaagcgcg tggctttgat gtgcaattcc aatttgcccc
    10021 attgggcggc gcggcttacg atgaacacgg cgcaccttac cc
//
LOCUS       DAOMVK010000020         9726 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_1754_length_9726_cov_2.893393, whole genome shotgun sequence.
ACCESSION   DAOMVK010000020 DAOMVK010000000
VERSION     DAOMVK010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 9726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9726
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1754_length_9726_cov_2.893393"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="PK856_01500"
     CDS             <1..708
                     /locus_tag="PK856_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HRL01077.1"
                     /translation="FLCMFIGYLLAKRLVPKYSMLILLIIAVVVTLVGGGWTSSGIDF
                     SLAQPSWMAPSFDWLAIVNLSLPLYVLTMLSQNLPGVAMLKSHGYEAPVKPVLIGTGL
                     LNMAFAPLGGFSVNLAAISAAICMNEHVDTDSKQRYRAAIWAGVFYVIAGLWATAVVA
                     LFLALPKDVSNMLAGFALLGTLLMCLNISFREGGFQEAALMTFLITLSGVTFLGIGST
                     LWGLLTGLVLVRFSPTK"
     gene            826..1353
                     /locus_tag="PK856_01505"
     CDS             826..1353
                     /locus_tag="PK856_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01078.1"
                     /translation="MSLYFEEIIKKYKSELFSPVNQWVDYEDENRRIICLSGRVVKNH
                     AANLSPPVLNVVVDINKKYIVGHYLTFPIKHHRFLTINRYLEFNLNKEESFFSNFYEK
                     IYFLDSGKDVLIETYIHGENLFCSSISYLDEFNEQGVKAFENIKMSYCDLDEYLKAAG
                     VNERKALKKISGFYN"
     gene            complement(1430..2536)
                     /locus_tag="PK856_01510"
     CDS             complement(1430..2536)
                     /locus_tag="PK856_01510"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRL01079.1"
                     /translation="MNAFWHYYVLTFKRIFGNGSVLVTMVLSVVFYSFFYPAAYQSQQ
                     GGLLPVVVVDEAPSQLSARLINAVEKNENVEVVAVVSQFSDATSMLKQQQADAIVLLP
                     AHMENNLRQGGEGLGVYVSTAYFLRTKQIAAGLVGSMASVVNEYLARFSSASAYKPEQ
                     LIHPLPLFNPLSGYGSYIVPAVAPLIIHQTILLGLSMLVAGWRETGWRLRKGEFAGMV
                     GSVMTIGCLGCLYLFGFMFWLNDYPRAGNFSLMLIAIPLFVLAAIGLALVLASYLDMA
                     ERSGHLLVFTSVPLFLLSGVAWPLQAMPAWLAGFSSSLPSTVGINTFVRINQMGVTGS
                     DVVWQLVYLGLVGIGLNAWAYKRLRGASKLQNDN"
     gene            complement(2533..3663)
                     /locus_tag="PK856_01515"
     CDS             complement(2533..3663)
                     /locus_tag="PK856_01515"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRL01080.1"
                     /translation="MWRVWRREWAYLWREPWDFCLLMLAPLLVIVVFASMFYAGKAEH
                     LPIALVDQDHSQLSRTIYKHLSLNHTVYIALQTDSMAEVEQAINRTEVWGYVLIPKGA
                     EAKMAALQDPSIAIAYNQSYFSIGNSISIAMQLSTVEAMADYVRNGYLSQNIPALHGP
                     MPGIKLSTLYNPDMSYELFLEPFVVPAVLHLLLCCCVAFTIGQEQKRGTMRSWLQAAS
                     IPQALLGKVLLYVTVFWLWTGVWLVWLVGIRGWFVAGSLALLLLAMFLFYLAYALIAA
                     ALVLLTQDMARTFGILAVYGGSSLSFAGVTLPLNNAPWFTQFWSSIIPYTPYIRLQTE
                     QWVVGSPVSTSLPHLAALLLYCALWGGLAYWGLQRMIKREAV"
     gene            complement(3663..4733)
                     /locus_tag="PK856_01520"
     CDS             complement(3663..4733)
                     /locus_tag="PK856_01520"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRL01081.1"
                     /translation="MNETQQTQETQETQETEPHPEANAVEPTSGGKKKMLLGLVLLLV
                     LGVIAFGLYQAFKPQEVMLQGRVEADTVLVSTKLPSRIESIKVEEGQTVKAKQALAIL
                     SSPEVEAKKQQAQASLQSALAMQATAERGAQQENIDSLYANWQSVLAQANLASSSHKR
                     ASVLYKEGVISRQRLEELAAANLSANALAEAAKQQYLRAKRGSTSEQQTIADSHVSIA
                     QAAVKETEALEAETQLFSPVDGVVANVYGHNTEFVAPGIPVVAILASKSMKVDLTVRE
                     DQYKTLYQRKQIEGYVPALDKSFVFNIKSIDAEGEFSTIKNTRQSGGYDIRSFKFHLE
                     PQTPIAELKVGMSVVFKVQQAR"
     gene            complement(4747..6207)
                     /locus_tag="PK856_01525"
     CDS             complement(4747..6207)
                     /locus_tag="PK856_01525"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRL01082.1"
                     /translation="MRKKQTLLLSALCAWSLQWAQADALSYEQAESHYLEMGHAGKAS
                     AALQQASELQAQAVKHLGMPRVDLNVRAYKLHAENEVPLDGIKRHLTDTIHDNIGKRV
                     DQLQSDLGIPPVVAEGLEDAATQAVDEGIATIPDQTEVRVDDSRVSSSLSMTWPIYTG
                     GIIHSTQKIAQINADKASISLEQQLDMERLELVQMYFGMQLNQQLTQVAAANLAGMQQ
                     HVKNALALERQGFISKGQRMQFEVARNTSERLYNNSKVALQNSRFELLNALQSRDNVS
                     LSTPLFVNTERHRTIDTWLQSYPDGSRLLQKTQLDTDIAKQNITIQKAAFKPKVFAFG
                     EYALDADKKWIVGLGLSYNLISGLDRRKSTQAAESTYLAAQLSKERVQQEIEKTIFKA
                     YSEMEVSQQNHTLLKENMLAAIENRRIQTISFREDSGTATQVIDAQNMVASLKAEQAL
                     NAYKYIMALAVLLNSTGNMGQFKQYTQAKHTDYIHD"
     gene            6413..7522
                     /locus_tag="PK856_01530"
     CDS             6413..7522
                     /locus_tag="PK856_01530"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01083.1"
                     /translation="MKHFNILDLDTMGQGVAKADGQITFIAKTLPNEVVAAEVYRNKK
                     NIHFAHMTEVLTPAPERQEAPCPHYAQCGGCDYQHVDYPTELAFKQKALRRHLRHFPD
                     VDITVHAAPQRWHYRNRIQLHYHADKKVMGLMDVNYEPLPVQNCQLIQADVREKMLEL
                     YQDNAWLDLLQDEPKRGHLELYCLNEEVQVSVNEPYANGGFTQVFPHMNHYLTTWLAH
                     QVNQYVADNAVIYDLFGGNGNLSLPLSRPTVVVDQYGEHTPQNNDSQTFVSQNLYAKD
                     AIQRLQAAIPAELNQPTCLIIDPPRSGLKNLKDFVATFKPKAVIFIACEPTSFVRDMA
                     TCLEHYTLMKVDLFDLFPTTQHYETVGVLVLNELV"
     gene            7509..8114
                     /locus_tag="PK856_01535"
     CDS             7509..8114
                     /locus_tag="PK856_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HRL01084.1"
                     /translation="MSLFEANHRANLIPQDGDLNDYGCVLDLQAALEWFETLLEKTPW
                     QHDEVVVFGKRITTARKVAWYGDAPYYYSGALKQPLPWSEAMLALKALVETHTGHSFN
                     SCLVNLYENGSQGVGWHSDDETTLGSAPTIASLSLGASRRFCFKHKINGKKCELTLAS
                     GQLILMHGDLQRYWLHAIPKMAGVTEPRINLTFRCIHTVSV"
     gene            complement(8245..8682)
                     /locus_tag="PK856_01540"
     CDS             complement(8245..8682)
                     /locus_tag="PK856_01540"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRL01085.1"
                     /translation="MSQRFDMETVAPVGYKAMIGLEMYMAKTSLNHTQKELIKLRASQ
                     INGCAFCLDMHSKDALKYGETMQRIVLLNAWPEAEVFSAEEKALLAMVEEITLISEHG
                     LSDETYAVAQQFFSETQIGEIIMAIVVINAWNRIAISTNKALG"
     gene            8914..9276
                     /locus_tag="PK856_01545"
     CDS             8914..9276
                     /locus_tag="PK856_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01086.1"
                     /translation="MLPVEEAIKNTNYYESIEQFIDFESNEILMDDVWLWRSKEGVVE
                     KLSGCSDYDIRKMLCLIIEQIFLKGGFPIDEDFGLAKPLLSEIEGLSINEKAVKLAHF
                     QMDNPDYGEDGNGIWFFS"
BASE COUNT         2554 a         2283 c         2230 g         2659 t
ORIGIN      
        1 ttcttgtgca tgttcattgg ctacttgctt gccaaacgct tggtgcccaa atacagcatg
       61 ctgattttgt taatcatagc cgtggttgtg acgcttgttg gcggcggttg gacgagctcg
      121 gggattgatt ttagcttggc ccagcccagc tggatggcac ccagttttga ttggttggcc
      181 atcgtgaatt taagtttgcc tttgtatgtg ttgacgatgt tgtcacaaaa cctgcctggt
      241 gtggccatgc tcaaaagcca cggctatgaa gcacctgtaa aacctgtgct catcggaacg
      301 ggtttgttaa acatggcatt tgcgccattg ggcggcttta gtgtgaattt ggctgcgatt
      361 tcagccgcca tttgcatgaa tgagcatgtg gatacagatt ccaagcagcg gtatcgcgct
      421 gccatttggg cgggtgtttt ctatgtgatt gcagggttgt gggccacagc agttgttgcc
      481 ttgtttttgg ccttgcccaa agacgtgtcc aatatgttgg caggctttgc cctactgggc
      541 actttgttga tgtgcttgaa catttccttc cgtgaaggcg gatttcaaga agccgcttta
      601 atgacttttt taatcacctt gtctggggtg acttttcttg gcataggctc tacgctgtgg
      661 ggcttgctaa cgggcttggt gttagtgcgt ttcagcccta ccaaataagg cagtcttcca
      721 ctattaaatg atataagcgg cctgtaaaaa cttacaggcc gcttatttga tacaacaagc
      781 aagtgttaga catacaaatg ttagacataa tagaggatta aatccatgtc gctatatttt
      841 gaagaaatta tcaagaaata caaaagtgaa ttattttctc ctgttaatca atgggtggat
      901 tatgaagatg aaaatagaag aataatttgc ctgtctggtc gagttgtgaa aaaccacgct
      961 gccaatcttt cacctcctgt tttaaatgta gttgtggata ttaataagaa atacattgtt
     1021 gggcattatt tgacgtttcc tattaaacac catagatttt taactattaa tagatattta
     1081 gaatttaatt taaacaagga agaatcattt ttctccaatt tttatgaaaa aatttatttt
     1141 cttgatagcg gtaaagatgt gttgattgag acttatattc atggtgaaaa tttattttgc
     1201 tcatctattt cttatcttga tgagtttaat gagcaggggg tgaaagcatt tgaaaatatt
     1261 aagatgagtt attgtgattt ggatgaatat ttaaaggccg ctggtgtaaa tgaaagaaag
     1321 gcattgaaga aaataagtgg tttctataat taatgaataa ttttttcatt atttgcgttg
     1381 attagatatt tccggagata aatagacaca acaaatattt atgctaaggc taattgtcat
     1441 tttggagctt ggaagcccca cgtaatcttt tgtaagccca tgcattgagt cctataccca
     1501 ccaatcccaa atacaccaat tgccatacca catcgctgcc agtcacgccc atttggttga
     1561 tgcgcacaaa agtattgatg ccaacagtgg atggcaatga ggaggaaaac cctgccaacc
     1621 acgcaggcat cgcttgcaaa ggccacgcca cgcctgagag caaaaacagt ggcactgaag
     1681 tgaaaaccaa caaatgccct gaacgctcgg ccatgtccaa atagctcgcc agcaccaaag
     1741 ccaaacctat tgctgccaat acaaacaagg gaatggcaat cagcattaag ctaaaatttc
     1801 ccgcgcgagg atagtcattc agccaaaaca tgaagccaaa caaatacaag cagcccaaac
     1861 agcctatggt catcacactg cccaccatgc ccgcaaattc ccctttgcgc aaacgccatc
     1921 ctgtttcgcg ccaacctgct accagcatgc tcaagcccaa taagatggtt tggtgaatga
     1981 ttaatggggc cacggcaggt acgatgtagc tgccatagcc agacaaaggg ttgaacaatg
     2041 gcaatgggtg tatcaactgc tcaggcttgt aagcactggc tgaggaaaag cgggccaggt
     2101 attcgttgac taccgatgcc atcgagccca ccaatcccgc agctatctgt ttggtacgca
     2161 gaaaataagc cgtgctcaca tacacgccca agccttctcc accttggcgc aaattgtttt
     2221 ccatgtgtgc aggcaaaagc acaatggcat cggcttgttg ttgtttgagc atggaagtgg
     2281 cgtcactgaa ctggctcacc acagccacca cttccacgtt ttcatttttt tctaccgcat
     2341 tgatgaggcg ggcagacagc tgactgggcg cttcatccac cactaccact ggcaataagc
     2401 ctccttgttg tgattggtag gctgcaggat aaaaaaaact gtagaaaacc acagacaaaa
     2461 ccatggtcac caaaaccgag ccattgccga agatgcgttt gaaggtgagc acataatagt
     2521 gccaaaatgc attcataccg cctccctttt gatcatgcgc tgcaaacccc aataggccaa
     2581 gcctccccac agagcacaat aaagcagcaa tgccgccaaa tggggcaaag aagtcgacac
     2641 agggctgcca accacccatt gttcagtttg caagcgaatg taaggggtat agggaatgat
     2701 ggaagaccaa aactgggtaa accaaggtgc gttgttcaag ggtaaagtca cccctgcgaa
     2761 actcaaagat gagcctccat acaccgccaa tatgccaaag gtacgagcca tgtcttgggt
     2821 aagcaaaacc aaagctgctg caatgagtgc ataggccagg taaaacaaaa acatggccaa
     2881 caataagagg gccaagctgc ccgcgacaaa ccagcctctt atgcccacaa gccaaaccaa
     2941 ccacacgccc gtccacagcc aaaacacggt gacatacaac agaactttgc ctaacagtgc
     3001 ttgaggaata gatgcggcct gtaaccatga ccgcatcgta cctctttttt gctcttggcc
     3061 tatggtaaag gccacgcaac aacacagcag caaatgcaac acagcaggca ccacaaacgg
     3121 ctctaaaaac aattcataac tcatgtcggg attgtacaaa gtagacagct tgatgccagg
     3181 catagggcca tgtaaggcag gaatgttttg gctcaaatag ccattgcgga catagtctgc
     3241 cattgcctcg acagtgctca actgcatggc gatggaaatg ctgttaccaa tgctgaaata
     3301 cgattggttg taggcaatgg caatgcttgg gtcttgcaat gccgccatct tcgcttcagc
     3361 ccctttgggt atcaacacat atccccacac ttcggttcgg ttgatggcct gttccacttc
     3421 cgccatggag tcggtttgta aggcgatgta tacggtgtga ttcagtgata agtgtttgta
     3481 aatggtgcgg ctcaattggc tgtggtcttg gtctaccaat gcaatgggca aatgctctgc
     3541 tttacccgcg taaaacatgc tcgcaaacac cacaatcacc agcaagggag ccagcatcag
     3601 caagcaaaag tcccaaggct cgcgccacaa ataagcccac tcgcgtcgcc atacccgcca
     3661 cattatcggg cctgctgcac tttaaacacc acactcatgc ccaccttaag ctctgcaatc
     3721 ggtgtttgtg gctctaaatg aaacttgaag ctgcggatgt cgtaacctcc gctttggcgg
     3781 gtgtttttga tggtggaaaa ctcaccttcg gcatctatgc ttttgatgtt aaacacaaag
     3841 gatttgtcca atgcaggcac ataaccttcg atttgcttgc gttggtacag ggttttgtat
     3901 tggtcttccc gcacagtcaa atccactttc atgcttttgc tcgccaaaat cgccaccaca
     3961 ggaatgccag gcgccacaaa ttcagtgttg tgcccataca cattggccac cactccatcg
     4021 acggggctga acaattgggt ttccgcttcc agtgcttcgg tttctttgac tgctgcttgg
     4081 gcaatggaga catgtgagtc ggcaatggtt tgttgttcag aggtgctgcc tcgtttggca
     4141 cgcaaatact gttgtttggc tgcttcagcc agagcattgg cagacagatt ggccgctgcc
     4201 aattcttcca gtctttgtct tgaaatcaca ccttctttgt acaaaacact ggcgcgtttg
     4261 tggctgctgc tggccaaatt tgcttgagcc aaaacgcttt gccaattggc atacagtgag
     4321 tctatgttct cttgttgcgc gccgcgctct gctgtggctt gcattgccaa agcggactgt
     4381 aaagatgctt gtgcctgttg cttcttggcc tcgacttcgg gactggataa aatggccaaa
     4441 gcttgcttgg ccttaaccgt ttggccttct tcaaccttaa tcgattcaat tcggctgggc
     4501 aatttggtgc tcaccaacac cgtatccgct tccaccctac cctgaagcat gacttcttga
     4561 ggcttgaacg cttggtacaa gccaaaagct atgacaccca ataccaaaag caataccagc
     4621 cccaacagca ttttcttttt accgccagag gtaggctcga cagcattggc ttcaggatgg
     4681 ggttctgtct cttgggtctc ttgggtctct tgggtttgtt gtgtttcgtt catcgccttg
     4741 tcctgctcaa tcatgaatgt agtccgtgtg cttggcttgt gtgtattgtt tgaattgccc
     4801 catattgcct gtgctgttaa gcaacacagc caaagccatg atgtatttgt aagcattgag
     4861 tgcttgctcg gctttcaagg aggcaaccat gttttgggca tcaatcactt gggtggccgt
     4921 gcctgagtct tcacgaaagg agatggtttg aatgcggcga ttctcaatgg ccgccaacat
     4981 attttccttt agcaaggtgt ggttttgctg tgagacttcc atttcagaat aggctttgaa
     5041 aatggttttt tcaatttcct gttgcacccg ctctttggat aattgggcgg cgagataagt
     5101 cgattctgcg gcctgcgtac tcttgcgtct gtccaaacct gatatcaggt tatagctcaa
     5161 acccaaacca acaatccatt ttttgtcagc atccaatgcg tattcaccaa aggcgaagac
     5221 tttgggctta aaggccgctt tttggatggt gatgttttgt ttggcaatgt ctgtgtccaa
     5281 ttgcgttttt tggagcaaac gggaaccatc gggatacgat tgcaaccatg tatcaatcgt
     5341 gcgatggcgt tctgtattga cgaacaatgg ggtcgacaag ctgacgttgt cacggctttg
     5401 caaggcattg agcaattcaa aacggctgtt ttgcaaggcc actttgctgt tattgtacaa
     5461 gcgctcgctg gtattgcgcg ctacttcaaa ctgcatgcgc tggcctttgc tgatgaagcc
     5521 ttgtcgctct aaggccaatg cattttttac atgctgctgc atgcccgcca aattggctgc
     5581 tgctacttgg gtcagctgtt gattcaactg catgccaaaa tacatttgca ccaattccaa
     5641 tcgctccatg tccaattgct gctcaagact gatggatgct ttatcggcat tgatttgggc
     5701 aattttttgg gtgctgtgta tgatgccacc agtgtaaatt ggccaagtca tggacaatga
     5761 tgaactgaca cggctgtcgt ccacccgaac ttcggtttgg tcagggatgg tggctatgcc
     5821 ttcgtccaca gcttgtgtgg ccgcatcttc taaaccctca gccaccacag gcggaatgcc
     5881 taagtcactt tgtaattggt caacacgctt accgatattg tcatgtatgg tgtcggtaag
     5941 gtgtcttttg atgccatcta agggaacctc attttcagca tgaagcttat aagcacgcac
     6001 atttaaatcg actcttggca tgcccagatg ttttaccgct tgagcctgta attccgatgc
     6061 ttgctgtaag gccgcactgg ctttgcctgc atgccccatc tccagataat gggattccgc
     6121 ttgttcatag cttaaagcat cggcctgagc ccattgcaat gaccatgcac aaagcgcgct
     6181 cagcagaagt gtttgtttct tacgcatttt gttccaagga gtacacgatg catgcccatg
     6241 cctttacaac aaaacaaggc tggcttggtg tacattccct ttcttaaaaa actttaacga
     6301 tagcctaatt ttacccgttt tgccttttac catgtggttt tttgccgatg gcttgctcgg
     6361 ttttgcctta caatagcggc aaattattta tagaaacaga gtttgcactg ccatgaagca
     6421 ttttaacatc ctcgatttag acaccatggg acaaggcgtg gccaaagctg atggccaaat
     6481 caccttcatc gccaaaaccc tgccaaacga ggtcgttgcc gcagaagttt accgcaataa
     6541 gaaaaacatc catttcgctc acatgaccga agtgttaaca cccgcacctg agcgtcaaga
     6601 agcgccttgc ccacattatg cccaatgtgg cggctgtgat taccaacatg tggattaccc
     6661 taccgaattg gcgttcaagc aaaaagcctt gcgccgtcat ttgcgccatt ttcccgatgt
     6721 agacatcacc gtccatgccg caccgcaacg ctggcattac cgcaaccgca ttcaattgca
     6781 ctaccacgcc gacaaaaaag tcatgggttt gatggatgtg aattacgagc ctttgccggt
     6841 gcaaaattgc caattgatac aggccgatgt gcgcgaaaaa atgttggagt tatatcaaga
     6901 caatgcatgg ctggatttgc tgcaagatga gcccaaacgt ggccacttag aattgtattg
     6961 cctgaatgaa gaagtgcaag tgagcgtgaa cgagccttat gccaatggcg gcttcaccca
     7021 agtgtttccg cacatgaatc actatctcac cacttggctt gcccaccaag tcaaccaata
     7081 cgttgccgac aatgcggtga tttatgactt gtttggtggc aatggcaatt tgagcttgcc
     7141 gctgagtcgc cccaccgtag tggtagacca atatggcgag cacacaccgc aaaacaacga
     7201 cagccaaacc tttgtgtcgc aaaatttata tgccaaagac gcgattcagc gtttacaggc
     7261 cgccattcct gcagaattga accaacccac ttgtctcatc atagacccgc cgcgttcagg
     7321 tttgaaaaac ctcaaagact ttgtcgccac cttcaaaccc aaagcagtga ttttcatcgc
     7381 ctgtgagccc accagttttg tgcgcgacat ggccacatgt ttggagcact ataccttgat
     7441 gaaagtggat ttgttcgatt tgttccccac cacccaacac tacgaaaccg tgggcgtgtt
     7501 ggtgctcaat gagcttgttt gaggccaatc accgtgccaa cctcatcccg caagatgggg
     7561 atttgaacga ttacggctgc gtattggatt tacaggccgc attagagtgg tttgaaacct
     7621 tacttgaaaa aacaccgtgg caacacgatg aggtggtggt gtttggcaag cgcatcacca
     7681 ccgcgcgtaa agtcgcatgg tatggcgatg cgccctatta ttacagcggc gcgctcaagc
     7741 agccactgcc ttggagcgag gccatgttgg ccttgaaagc tttggtggaa acacatacag
     7801 gccacagctt caattcttgt ttggtcaatc tgtatgaaaa tggcagccaa ggcgtgggct
     7861 ggcacagcga tgacgaaacc actttgggca gcgcgcctac catcgcctca ttgagtttgg
     7921 gcgcgagtcg gcgcttttgc ttcaagcaca aaatcaatgg caaaaaatgt gaattgacct
     7981 tggcgtctgg ccaattgatt ttgatgcacg gtgatttaca acgctattgg ctgcatgcga
     8041 taccgaaaat ggcaggtgtc actgagccac gtatcaattt gacgtttcgc tgcatacaca
     8101 cagtttctgt ttaaaccaac gctttagcaa tacaagcggc ctgtaaacct tatgatatat
     8161 ttacaggccg ctgtttatgt ctgattacat cttgtctatg cggagattca cctatatcca
     8221 cacagcgaat agcatcaact gaatttagcc caaagcctta ttagtactga tggcaatacg
     8281 gttccaagca ttgatgacca cgattgccat gatgatttca ccgatttggg tttcgctgaa
     8341 aaactgttgt gccacagcat aggtttcatc gctcaaaccg tgctcgctga tgagggtgat
     8401 ttcttccacc atagccaaca aggctttctc ttcggcgctg aacacctcgg cttcaggcca
     8461 cgcattcagc aacacaatgc gctgcatggt ttcaccgtat ttcagcgcgt ctttgctgtg
     8521 catgtccaaa caaaacgcgc agccattgat ttgtgaggcg cgcagcttaa tcaattcttt
     8581 ttgggtgtgg ttgaggctgg ttttcgccat atacatctct aaacctatca tcgctttata
     8641 gcccactggt gcgacggttt ccatgtcaaa acgttggctc atgttgttgc tccattcagt
     8701 gttggttcga ttgtataatc aatcaagagt ttttgagttg tggccgcaag caccagttca
     8761 atccctgagt ggatttattt tggctagcaa acgtcattca tctaaccgat tcttaattga
     8821 tgccactgtc agttaaaagt gccaatcaaa agccagttta tgattagtca gggagcatca
     8881 tcaagaaata gcatcatatt tagggagtca gttatgttac cagtggaaga agccataaaa
     8941 aataccaatt attatgaaag tatcgaacaa ttcatagatt tcgagagtaa cgaaattcta
     9001 atggatgatg tttggctttg gcgaagcaaa gaaggggttg tggaaaaatt atcaggctgc
     9061 tctgattacg atataagaaa aatgctgtgt ttaatcattg agcaaatatt cttaaaagga
     9121 ggctttccta tagatgaaga ctttggactg gctaagccat tattgtctga gattgaaggc
     9181 ttaagcatta atgaaaaggc agtgaaattg gctcacttcc aaatggataa tcccgactat
     9241 ggtgaggatg gaaatggcat atggtttttt tcctaaaaac tgtaatgagg caatgaggtt
     9301 gacactcttt catataagct catctctgat gacaattcct gatggatgaa atgtcacaac
     9361 aaggccgaat aaggcattgt gaaaagcatg caaatgtttc aagtaatgtc tttgtaagca
     9421 tgggctgctt tattgagatg gccgaaaaat aagtcaaaga atgctgaaaa aatctgtcag
     9481 agctacactt caatgccatg gttttggcaa ctcaccgcaa tgtttgaatc agtcaatgat
     9541 tttggtttta aaacacaaat aagcggcctg taaactttac aggccgcttg tatcacaaca
     9601 ctttcaatca gaattatttg gtggtgtcac gcacttgttt gtcacctggc acacccaagc
     9661 cagaccaacg catgatgttg gccacttcaa attcgttcaa ttcgtagaag ccaccgcgtt
     9721 tcaagc
//
LOCUS       DAOMVK010000021         9697 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_1762_length_9697_cov_3.464841, whole genome shotgun sequence.
ACCESSION   DAOMVK010000021 DAOMVK010000000
VERSION     DAOMVK010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 9697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9697
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1762_length_9697_cov_3.464841"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..1410)
                     /locus_tag="PK856_01550"
     CDS             complement(100..1410)
                     /locus_tag="PK856_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HRL01087.1"
                     /translation="MSRTTLIHAGWILPMADTPVVLREHALVLKDSRIEAIVTADEAK
                     SVVADEVLHLPQHVLMPGLVNLHGHSAMTLLRGLADDLALMDWLNNHIWPAEGKHVSD
                     AFVHDGSVLAMAEMLLGGTTTINDMYFYHGAVARAGLKAGMRTFVGCSILEFPTNYAQ
                     SSVQYIEKSLAEAQEFVGEDLVKFVWAPHAPYTVSDDTFCKVVELADKNDWLIHCHIH
                     ETVFEVESSVKEHGKRPLARLDDLGVLNSRLIAAHMVHTNVEEVALAAERGISVAHNP
                     ASNMKLASGCAPIQAMLDAGVNVGLGTDGAASNNKLDMFADMRLGALIAKGFSQNPTD
                     VKAYDVLKMATINGAKALHMGDKIGSLEVGKQADVIALDLSAIATQPVFDPISHLVYA
                     ADRNQVTHVWVNGEAVVQERVLTRLNLVELLSNAQEWQQKIMAK"
     gene            complement(1423..3033)
                     /locus_tag="PK856_01555"
     CDS             complement(1423..3033)
                     /locus_tag="PK856_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRL01088.1"
                     /translation="MLLAFVWLWPGIVDHDLWKPNEPVIHAAIQSILAGGNVWAPEVA
                     GQAYVAQPPLYAWIGGLFQAAFSPWLMSEFTAIRLTTVLFMSIGLACAGGAGRHLLGK
                     RHGRSVVLILIGCVGLMLAGHRMDTWAITFAGFAMSLYAMTRANDHTALAGFGLALGW
                     FLVFMSSSLSELILVMVIAMGMMVFARWRSRRYAIILVMALMMAVPLVSIWPYAMYQT
                     DPAVFQLWWNDYAFGGFGGWEHSAIGHEFGYYFEVALWYVLPAWPLALWTFIKRDKVD
                     HRVWQVCGVWLLIGILWLGLMRKTYTENALILVLPLAVLGAAQLDSLRRGAVAFLNWF
                     GVMLFGALAIFLWLGFAAMNFGWPAKLAERSAYFSPYYRPDFDYFPIVIAILFTLLWL
                     WAVTRKHVRGRQAVTNWAAGMTLGWSLLMTLFLPWLDAAKSYRPVVEQFETALHPNTL
                     QRLKSGQDCLFIDAQSNLSAVLAWQVYSKVTINTQDAQTCQYHLLKHKADDAQVAGGW
                     QILWQGHRPREDYEHFILVHKQQFDALF"
     gene            complement(3221..3436)
                     /gene="rpmE"
                     /locus_tag="PK856_01560"
     CDS             complement(3221..3436)
                     /gene="rpmE"
                     /locus_tag="PK856_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HRL01089.1"
                     /translation="MKADIQPNYHEVKVTCSCGNAFVTKSTMDKENFAIEVCSECHPF
                     YTGKQKIVDTAGRVDRFNQKFGNMFKR"
     gene            complement(3533..4066)
                     /locus_tag="PK856_01565"
     CDS             complement(3533..4066)
                     /locus_tag="PK856_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01090.1"
                     /translation="MKKMYLGALLVLGATSWAQAQTPQQWEQCAERVGRSVDVQETGQ
                     VGYELAIKDKCGVRPISNAGMAKPDGKLPFDVVQAAPWKGRFQQLTGKTYGSIKESLA
                     VSSAMQRQGDWLVGSGYDPRGAGATKAAIAVNTKTGKVMAAYASSDGVKFFGFNENSK
                     GVPDKLWQWVSEETAAG"
     gene            complement(4114..5532)
                     /gene="cysS"
                     /locus_tag="PK856_01570"
     CDS             complement(4114..5532)
                     /gene="cysS"
                     /locus_tag="PK856_01570"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRL01091.1"
                     /translation="MLTVYNTLTRQKEQFKPLDPNHVRMYVCGMTVYDYCHLGHARVM
                     VVFDMVARWLRESNYPLTYVRNITDIDDKIIARAAQNGETIGELTARFIQAMDEDAAA
                     LGVQKPDVEPKATEHIKQMIEMIERLIANGKAYPADNGDVYYAVREFPAYGQLSGKSL
                     DDLRAGERVEVDSFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMGEEMFG
                     RVFDIHGGGADLQFPHHENEIAQSCGATGVCDHSHDDLAGKHTQSHVKYWLHNGFIRV
                     DNEKMSKSLGNFFTIREVLQKYDAEVVRFFILRAHYRSPLNYSDAHLDDAKGALTRLY
                     TTLKNTAAADVKVSADSNEYTQRFFAAMDNDFGTVEAMAVLFELAAEVNKSNDAHLAG
                     VLKALANILGLLERDPVAFLQGGAGSDDGLSNEAIDALIAERQAARAAKNWAESDRIR
                     DELTSAGIVLEDAAGVTTWRRQ"
     gene            complement(5703..6335)
                     /locus_tag="PK856_01575"
     CDS             complement(5703..6335)
                     /locus_tag="PK856_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HRL01092.1"
                     /translation="MNAQTSMVEQLLSQLQGAPMQQIASQLGADQQQTESAVGAALPM
                     LLGMLGNNANQGGGADALFGALMRDHAPAQAQGFGGGDLLGSVLGSVLGGGQGQNAGS
                     AILGHIFGGQQQQAESSLGQASGLGGGNAGQLLAILAPIVMSFLANKVQGNGLDAGGL
                     GQVLQQEQAQAQQQGSVGGSLLTSMLDQDGDGQLGAGDLFKLAGSFLNKR"
     gene            complement(6362..7504)
                     /gene="obgE"
                     /locus_tag="PK856_01580"
     CDS             complement(6362..7504)
                     /gene="obgE"
                     /locus_tag="PK856_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009119875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRL01093.1"
                     /translation="MKFIDEAKIEVVAGKGGNGASSFRREKFVPMGGPDGGDGGKGGS
                     VIAIADENINTLVEFRFVKKYMAKNGEKGHGADRYGKGADDIMLHMPVGTLIRDVDTD
                     EVVADLTVHGQEVVLAKGGKGGLGNIHFKSSTNRAPRKCTPGEEGINRLLHLELKVLA
                     DVGLLGMPNAGKSTLISAVSAARPKVADYPFTTLHPNLGVVRMDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLRHLSRTGLLLHVVDLAPFDEEVDPAAEALALAEELRKYDEALYD
                     KPRWFVLNKLDVVPEEERAERVAAFLAAVGWNHSTPDDEFEFDVETPRVFEISAVNRQ
                     GTADLVYAIHRYIQRKKELAAKLLAADEVDANAVVSTTDRNVLKPE"
     gene            complement(7667..7939)
                     /gene="rpmA"
                     /locus_tag="PK856_01585"
     CDS             complement(7667..7939)
                     /gene="rpmA"
                     /locus_tag="PK856_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRL01094.1"
                     /translation="MATKKAGGSSKNGRDSEAKRLGVKAFGSELIPAGSIIVRQRGTR
                     FHAGNNVGMGKDHTLFAKVDGYVEFTVKGALNRKTVNVVPYTGEAE"
     gene            complement(7958..8266)
                     /gene="rplU"
                     /locus_tag="PK856_01590"
     CDS             complement(7958..8266)
                     /gene="rplU"
                     /locus_tag="PK856_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRL01095.1"
                     /translation="MYAVIKTGGKQYKVTVGEKLKVEQIPAELDSQIVLEEVLMVADG
                     DNVQIGAPLVAGAKVTATVVSHGRHDKVRIFKMRRRKHYQKRQGHRQNFTEIRIDAIA
                     "
     gene            8531..9505
                     /locus_tag="PK856_01595"
     CDS             8531..9505
                     /locus_tag="PK856_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRL01096.1"
                     /translation="MLETVPYFQSHLEDDLARVNVVINKAVMSDVALISQIGQYIISA
                     GGKRLRPIMTILSGKVLGYDNEQLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRSTAN
                     NMFGNAAAVLVGDFLYTRAFQLMVHTKDMRILEVMADATNIIAEGEVMQLMNIGNTDI
                     SEAEYLQVIQYKTAKLFEAAAQVGALLAKATPEQERALKDYGMHVGTAFQIIDDILDY
                     SGDPEQIGKNVGDDLAEGKPTLPLIYLMRQGSEQAAKDVRHALEHADRDYFDAIYAHV
                     QNSDALAYCHQEAQKAVNNAVACLENLPDNEVRFAMRELAEQSIARLN"
BASE COUNT         2622 a         2745 c         1985 g         2345 t
ORIGIN      
        1 gaaattgtac caaaccttgt aacagtgttt acaggccgct tgaataaaca agccgctcaa
       61 atgagcggtt tgtttatttg ttttcatgtc aaatagaggt tacttagcca taattttttg
      121 ctgccattct tgggcattgc tcaaaagctc taccagattc aaacgtgtta acacacgctc
      181 ttgcaccact gcctcgccat tcacccacac atgggtcact tgattgcggt ctgctgcata
      241 gaccaaatgt gaaattggat caaacactgg ctgggtagca atggctgaca aatccaatgc
      301 aatcacatcg gcctgtttgc ccacttccaa tgaacctatc ttatcgccca tgtgcaaagc
      361 cttcgcacca ttgatggtgg ccattttcaa cacatcatag gctttcacat cggtcggatt
      421 ttggctaaag cctttggcta tcaacgcacc caagcgcatg tcggcaaaca tgtccaattt
      481 gttgttggaa gccgcaccat ccgtacccaa gcccacattc acacccgcat ccaacatcgc
      541 ttgtatcggc gcgcagcctg aggccaattt catgttagac gcagggttgt gcgccacact
      601 gatgccgcgt tctgcggcca acgccacctc ttctacattg gtgtgcacca tgtgcgccgc
      661 aatcaagcga gaattcaaca cacccaaatc atccaagcgc gccaatggac gcttaccatg
      721 ctcttttacc gagctttcca cctcaaaaac ggtttcatgg atgtggcaat gaatgagcca
      781 atcattttta tccgccagct ctaccacttt acagaaggta tcatctgaca cggtatatgg
      841 agcatgcggt gcccatacaa atttaaccaa gtcttcgccc acaaactctt gcgcctcagc
      901 caaagatttt tcaatgtatt gcactgagct ttgcgcgtaa ttcgtaggga attccaaaat
      961 actgcagccc acaaatgtgc gcatccctgc ttttaggccg gcacgggcca ctgctccgtg
     1021 gtagaaatac atatcgttaa tggtggtggt gccacccaac agcatttccg ccatcgccaa
     1081 taccgaacca tcgtgcacaa atgcgtcgct gacatgttta ccttctgctg gccaaatgtg
     1141 gttgttcaac caatccatca aggccaaatc atcggccaaa ccgcgcaaca aagtcatggc
     1201 gctgtggcca tgcaaattca ccaaacctgg catcaacaca tgttgtggca aatgcaaaac
     1261 ctcatcggcc accactgatt tcgcctcatc agcggtaacg atggcttcaa tacggctgtc
     1321 tttcaacacc agcgcatgct cacgtaaaac cacaggcgta tctgccatgg gcaaaatcca
     1381 acctgcatga ataagagtgg tacggctcat actgcttccc ttttaaaata aggcatcaaa
     1441 ctgctgtttg tgcaccaaaa taaagtgttc ataatcttca cgtggccgat ggccttgcca
     1501 caaaatttgc cagcctccag ccacttgtgc atcatctgct ttgtgcttga gtaaatggta
     1561 ttggcaagtt tgcgcatctt gcgtgttgat ggtgaccttg ctgtaaacct gccaggccaa
     1621 cacagccgac aagttgcttt gcgcatcaat aaacaggcaa tcttgaccag atttcaagcg
     1681 ctgtaaggta ttgggatgca aagcggtttc aaattgctca acaaccggcc tgtaactttt
     1741 ggcagcgtct aaccacggca aaaacagtgt catcaacaag ctccaaccca aagtcatgcc
     1801 tgctgcccaa ttggtcacag cttgacgtcc acgcacgtgt tttcgagtaa ccgcccacag
     1861 ccacagtaag gtgaacaata tggcaatgac aatggggaaa taatcaaaat caggccgata
     1921 atatggacta aaatacgctg aacgctcggc caatttggct ggccagccga aattcatggc
     1981 agcaaaaccc agccacagaa aaatagccaa agccccaaac aacatgaccc caaaccaatt
     2041 gagaaatgcc actgctccgc gacgcaatga atcgagctgc gcagcgccta ataccgctaa
     2101 aggcaacact aaaatcaatg cattttcagt ataggttttg cgcatcaagc ccaaccacaa
     2161 aataccaatg agcaaccata ccccgcaaac ctgccatact cgatgatcga ccttatcgcg
     2221 cttgatgaat gtccacaacg ccaacggcca agcaggcagc acataccaca aggccacctc
     2281 aaaataatag ccaaactcat gcccaatggc actgtgctcc cagccaccga atccaccaaa
     2341 cgcataatca ttccaccaaa gctgaaagac tgcaggatcg gtttgataca tggcgtaagg
     2401 ccaaatactc accaagggca ctgccatcat caaagccatc accaagatga tggcataacg
     2461 gcggctgcgc caacgtgcaa acaccatcat ccccatggca atgaccatca ccaaaatcaa
     2521 ctcactcaag cttgacgaca tgaataccaa aaaccagccc aaagccaaac caaaacccgc
     2581 caaagccgtg tgatcattgg cgcgggtcat ggcgtacaaa ctcatggcaa aaccggcaaa
     2641 ggtaatcgcc caagtgtcca tgcggtgccc cgccaacatc aaacccacac aacctatcag
     2701 tatcaacacc acactgcggc catggcgctt acccaacaaa tgccgaccag cgccaccagc
     2761 acaagccaag cctatgctca taaacaatac tgtcgtcaaa cgaatggcag taaactcgct
     2821 catcaaccat ggtgaaaatg cggcctgaaa caagcctcct atccacgcat acaaaggtgg
     2881 ttgcgccacg tatgcttgcc cagcaacttc aggtgcccaa acattacccc cagccaaaat
     2941 cgactgtatt gctgcatgta ttacaggttc attgggcttc cataaatcgt gatcgactat
     3001 gcctggccac aaccacacaa atgccaacaa caacaataac cagggctttt ccgtgggttt
     3061 gattgaaggc cttcgttcag gaggaatata agttaacatc gaaaacaccg cttaagaatc
     3121 gctgctcagt ttagcacttt caccaccgat tccaaactga gcagaaacag atggcaataa
     3181 aaaagacagc ctaagctgtc tttttggcag atgtaacgaa ttaacgtttg aacatattgc
     3241 cgaatttttg gttaaagcga tcaacacgac cagccgtgtc aacgattttt tgtttaccag
     3301 tgtaaaatgg atggcattca gaacaaactt caattgcaaa gttttctttg tccattgtag
     3361 acttggtgac aaaagcgtta ccgcaagaac aagtcacttt aacttcatgg tagttaggtt
     3421 gaatatcagc tttcatgtgc attccttgag ttggcgggta tagggattgt gcctatactg
     3481 cattaaacga atcggctatt atagtctcta tatttagcca aatcaaggat tttcaacctg
     3541 ccgcagtctc ttctgatacc cattgccaca gcttatctgg tacgcctttg gaattctcat
     3601 tgaatccaaa aaatttcacc ccatcgctgc ttgcataagc cgccatcacc ttgcccgttt
     3661 tggtgttcac ggcaatggcc gccttagtag cacccgctcc tcgcggatca taaccgctgc
     3721 ctactagcca atcaccttga cgctgcattg cactcgacac cgccagtgat tctttgatag
     3781 agccataggt tttacccgtt aattgctgaa aacgcccttt ccatggcgca gcttgcacca
     3841 catcaaatgg caatttccca tctggcttgg ccatgcctgc attggaaatg gggcgaacac
     3901 cacatttgtc tttaatcgcc aattcgtaac caacctgccc agtttcttgc acatccacac
     3961 tacggcccac gcgctcagca cattgctccc attgctgagg cgtttgtgct tgcgcccaag
     4021 aagtggcacc caacaccaac aaagcaccca aatacatttt tttcatactg gtctccttgt
     4081 taacaggccg cttgtcttaa cgctgttctt actttactga cgacgccaag tggtcacgcc
     4141 tgctgcatct tccaacacga ttcctgcaga tgttaactca tcgcgaatgc gatctgactc
     4201 tgcccaattt tttgcggcac gtgccgcttg acgctcggca atcaaagcgt caatcgcctc
     4261 attgctcaaa ccatcgtcac tgcctgcacc accttgcaag aatgccacag ggtcacgctc
     4321 tagcaaaccc aaaatattgg ctaaggcttt taacacgccc gcaaggtgcg catcattaga
     4381 cttgttcact tcagcagcca actcaaacaa caccgccatc gcttctaccg taccgaaatc
     4441 gttgtccatc gccgcaaaaa agcgctgcgt gtattcattg ctgtccgcgc tcaccttcac
     4501 atcggcagcg gcggtgtttt tcagagtggt gtacaaacgg gttaatgcgc ctttggcatc
     4561 gtccaaatgg gcatcagagt aattcaatgg actgcggtaa tgtgcacgca aaataaagaa
     4621 gcgcaccact tcagcatcgt atttttgcaa gacttcacgg atggtgaaaa aattacccag
     4681 agatttggac attttctcgt tgtccacgcg aataaagcca ttgtgcagcc agtatttcac
     4741 atggctttgc gtgtgtttgc ccgccaaatc atcatgactg tggtcgcaca cacccgtcgc
     4801 gccgcaactt tgggcgattt cgttttcatg gtgagggaat tgcaagtctg cgccaccacc
     4861 gtggatgtca aacacgcgac caaacatttc ctcacccatg gctgaacatt caatatgcca
     4921 acctgggcga ccattacccc aagggctttc ccaagcaggt tcgcctgctt tggcggcttt
     4981 ccacaacacg aaatccaatg ggtcgcgctt gaaactgtct acttccacac gctcacctgc
     5041 gcgcaaatca tccaaagatt tgcccgacaa ttggccataa gctggaaact cgcgcacagc
     5101 gtaatacaca tcgccattgt cagcaggata ggctttgcca ttggcaatca aacgttcaat
     5161 catctctatc atttgcttga tgtgctcagt ggctttaggc tccacatcgg gtttttgcac
     5221 acccaaagct gctgcatctt cgtccatggc ttgaataaaa cgtgcggtca attcaccaat
     5281 ggtttcgcca ttttgcgccg cacgcgcgat gattttgtcg tcgatgtcgg taatgttgcg
     5341 cacataagtc aaaggatagt tagattcacg caaccaacgt gccaccatat caaacaccac
     5401 catcacccgc gcatgaccca agtgacagta gtcatacacc gtcatcccac acacatacat
     5461 gcgcacgtga ttgggatcta agggtttaaa ctgttctttt tgacgggtta aggtgttgta
     5521 tacggttaac atatcggtat gcttacattc ttgtgttcat gaagaaatct gattatacgg
     5581 tatttgtacg ctgcacaaac catagcatca ttctaaataa gatattttgc atgcaaatgc
     5641 ggcctgtaaa cacatttaca ggccgcatta actgcattaa atcaggctaa aaatcgctca
     5701 aatcaacgct tgttcaaaaa gctgccagcc aatttgaaca aatcacccgc gcccaattgg
     5761 ccatcgccat cttggtctag catgctggtc aacaaactgc cgcccacact gccttgctgc
     5821 tgtgcttgag cttgttcttg ttgcaatact tgacccaaac cacctgcatc caagccattg
     5881 ccttgcactt tattggccaa gaaagacatc acgatgggtg ccaaaatcgc caacaattgg
     5941 cccgcattgc cgccacccaa accagaagcc tggcccaaag aagattcggc ttgctgttgt
     6001 tggccgccaa agatgtggcc caaaatcgca gaaccagcgt tttgaccttg accaccaccc
     6061 aataccgagc ccaacacgct gcccaacaaa tcgccgccgc caaagccttg agcctgagca
     6121 ggcgcatggt cgcgcatcaa tgcaccgaac aacgcatcgg caccaccgcc ttggttggca
     6181 ttgttgccca acatacccag caacatcggc aaagccgcgc ccacagcact ttcggtttgc
     6241 tgttggtcag cgcccaattg cgatgcgatt tgttgcatcg gtgcgccctg taattgagac
     6301 aacaactgtt ccaccatcga tgtttgtgca ttcatgctgc ttactcctga gtgtaggggt
     6361 tttattccgg ttttaagaca ttgcggtctg ttgtgctcac aacagcgttt gcgtcaactt
     6421 catctgctgc tagcagtttt gctgccaatt cttttttgcg ctgaatataa cggtgtatcg
     6481 catacaccaa atctgccgtg ccttggcgat tgaccgctga gatttcaaac acccgtggtg
     6541 tttccacatc aaattcaaat tcatcgtctg gagtcgaatg attccaaccc acggccgcca
     6601 aaaatgccgc cacgcgctct gcgcgttctt cttcaggcac cacatccaat ttattcagca
     6661 caaaccaacg tggcttatcg tataaggctt catcgtattt tcgcaactct tcagctagag
     6721 ccaaagcctc agcagctgga tccacctcct catcaaatgg cgccaaatcc accacatgca
     6781 agagcaatcc tgtgcgtgac aaatggcgca agaagcggtg gcccaagcca gcgccttcag
     6841 ccgcaccttc aatcaagcct ggaatgtccg ccatcacaaa gctgtggttt tcatccatgc
     6901 gcaccacacc caaattcgga tgcaaagtcg tgaacggata atccgccact tttgggcgcg
     6961 ccgcagacac cgcactgatg agtgtagatt tacccgcatt gggcatgcca agcaaaccca
     7021 catcggccaa cactttcaat tccaaatgca acaaacggtt gatgccttct tcgccgggtg
     7081 tgcacttgcg tggtgcacgg ttggtagatg atttgaaatg gatgttgccc aagccacctt
     7141 tgccgccttt ggccaacacc acttcttgcc cgtgcacggt caaatcggcc accacttcat
     7201 cggtatccac atcgcgtatc aacgtaccaa ctggcatgtg caacatgatg tcgtccgcac
     7261 ccttgccata acggtcggca ccgtggcctt tttcgccgtt tttcgccatg tattttttca
     7321 caaaacgaaa ttccaccaag gtgttgatgt tttcatcggc aatcgcaatc acgctgccac
     7381 ctttgccacc gtcaccaccg tctgggccgc ccatgggcac aaatttctcg cggcgaaagc
     7441 tggaagcacc gttaccgccc ttgcctgcca ccacttcaat cttggcttcg tcaataaact
     7501 tcatgtgtaa acctttatta gtctgtattg cgtatggttt gtacaaaacc gattaagcgg
     7561 cctgtaagcc tataaatgcc cttacaggcc gcttaatcag tcttgatgca tattataaat
     7621 aaaaaaagcc ccatcatgcg acagggcttt ttcacattct aacgaattat tcagcttcgc
     7681 cagtgtaagg aacaacgttc acagttttac ggttcaaagc acctttaaca gtgaattcaa
     7741 cataaccgtc aactttagcg aacaaagtgt ggtctttacc catgcccaca ttgttaccag
     7801 cgtggaaacg agtaccacgt tgacgcacaa taatagaacc agctggaatc aactcgctac
     7861 cgaaggcttt aacgcccaag cgtttggctt ctgaatcgcg accgtttttt gagctaccgc
     7921 ctgctttttt ggttgccatt taattttact cctaagatta agcgattgcg tcgatgcgaa
     7981 tttcagtgaa attctgacga tgaccttggc gtttttggta gtgtttacga cgacgcattt
     8041 tgaaaatgcg tactttgtcg tgacgaccat gagaaaccac tgtagcagtg actttagcac
     8101 ctgcaaccaa tggagcacca atttgaacat tgtcaccgtc cgcaaccatc aatacttctt
     8161 caagtacgat ttggctgtcg agttcggctg gtatctgttc tacttttaat ttttcaccta
     8221 cggtaacttt gtactgttta ccgccggttt ttatgaccgc gtacatactc aactccagat
     8281 taataacatt ctgccaacac ggcaaaaaga atcggagatt ataagagatt tttctaagtt
     8341 cgtcaaaaat aaaacccaaa tcaagtgatt aagcggcctg caactgccaa tttgccacaa
     8401 atcttaacct gatacaccac attcacacca acactaaccc tctctgccca tggcaaaact
     8461 gtacagggcg ggcaacaatg ctataatcaa cgccgttgtt ttgcccccct cattatcaaa
     8521 agtcgccaat atgttagaaa cagtgcctta ttttcaaagc catttagaag atgatttagc
     8581 ccgtgtcaac gtggtcatca acaaagctgt gatgtcagac gtggcattga tttcacaaat
     8641 tggccaatac atcatcagcg caggtggcaa gcgtttgcgc cccatcatga cgattttgtc
     8701 gggcaaagta ttgggctatg acaatgagca attgtattca ctggccgcca tggtggagtt
     8761 catccacacg tctaccttat tacacgacga tgttgtcgat gaaagcgatt tgcgccgtgg
     8821 ccgctccacc gctaacaaca tgtttggcaa cgccgccgcc gtcttggtgg gtgacttctt
     8881 atacacccgc gccttccagt tgatggtaca caccaaagac atgcgcatct tggaagtgat
     8941 ggccgacgcc accaacatca tcgccgaagg cgaagtgatg caactcatga acatcggcaa
     9001 caccgacatc agcgaagccg aatacctgca agtcatccaa tacaaaaccg ccaaactgtt
     9061 tgaagccgcc gcacaagtgg gcgccttact ggccaaagcc acacccgaac aagagcgcgc
     9121 cctcaaagac tacggcatgc acgtgggcac cgccttccaa atcatcgatg acatattgga
     9181 ttactcaggc gacccagaac aaatcggcaa aaacgtcggc gacgacttgg ccgaaggcaa
     9241 accaaccttg ccattgatat acctgatgcg ccaaggctca gaacaagccg ccaaagacgt
     9301 gcgccacgcc ttggaacacg ccgaccgcga ctacttcgat gccatctatg cccatgtaca
     9361 aaactcagac gccttagcct attgccacca agaagcacaa aaagccgtga ataatgccgt
     9421 ggcttgcttg gaaaacctgc ccgacaatga agtccgcttc gccatgcgcg aactggccga
     9481 gcagtctatt gcccgtttga attaatgcca agcggcctgt aagcctctat cacagcaaaa
     9541 cactgcctca agagatttac aggccgctta tagtaagtac tgttgcaacc gctttaataa
     9601 atccgtaaac acgttgcacc acatccccgc tctcacacac tcgccatagt tcaacggata
     9661 gaacgtatgc ctcctaagcg taaaatacag gttcgat
//
LOCUS       DAOMVK010000022         9607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_1783_length_9607_cov_3.337940, whole genome shotgun sequence.
ACCESSION   DAOMVK010000022 DAOMVK010000000
VERSION     DAOMVK010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 9607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9607
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1783_length_9607_cov_3.337940"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..91)
                     /locus_tag="PK856_01600"
     tRNA            complement(1..91)
                     /locus_tag="PK856_01600"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(55..57),aa:Ser,seq:gga)
     gene            291..944
                     /locus_tag="PK856_01605"
     CDS             291..944
                     /locus_tag="PK856_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019956995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRL01097.1"
                     /translation="MKMWKTFSIVAVCSTLALSACVTDPVTGQTNRNNTVIGALGGAA
                     TCGIVGALTHGSKGARNSALACGAIGAGAGAYMDYQERQLRQSLANTQVQVDRVGDEI
                     RLVMPESITFATGSAALNSSSTSTLTSVADVLAKYTDTTIQVIGHTDSTGSDAINEPL
                     SRNRAAAVANFLSNRGVAANRISATGMGSRQPIASNTTADGKAQNRRVEIKVNPIQR"
     gene            complement(1136..2131)
                     /locus_tag="PK856_01610"
     CDS             complement(1136..2131)
                     /locus_tag="PK856_01610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HRL01098.1"
                     /translation="MKTSPANFNNGFLDNASKLTTIGLIMLIVSAVLLINTIETTFNQ
                     IWGANQPRPLLTRLLVYWALLTLGPLILGLTISQIGWFMRQSVFAFQYPNTAWILHFV
                     ISTLVNTALLWLMYRVVPARYVPSKHALAGALWSAIILELAKRIFSYYFSHFGSYEVI
                     YGAFAAIPLFLLWINVLWAILLSGAILTNALSYWQGDAFQRHAGQHGRFDDVLKILIL
                     LAQAQCQGSSLPTRAFRSHINMGYDELGELLEQLQHYHFVTRDNDGWLLKTNPYEINL
                     RDVFEIFVYRVKSNQDDHIGLMVQNFMQPSLSTFSMTLADFVQQSNLIKSPQQEA"
     gene            2406..>2818
                     /locus_tag="PK856_01615"
     CDS             2406..>2818
                     /locus_tag="PK856_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019956997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRL01099.1"
                     /translation="MLKILVLYYSQNGSVLNLAREIAKGIDSMAGCEAVLRTVPKVST
                     VCEQVADSIPDSGAPYVSIEDLRTCAGLAMGSPTRFGNMAAAMKYFLDGTSGVWLSGD
                     LIGKPASVFTSSSSMHGGQESTLLTMMVPLLHQGM"
     assembly_gap    2819..2844
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2845..3032
                     /locus_tag="PK856_01620"
     CDS             <2845..3032
                     /locus_tag="PK856_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019956997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HRL01100.1"
                     /translation="VPYSEADVGSTKTGGTPYGASHVSGSEGKNQLSPAEKRIAFAQG
                     KRLAQTALQLSLGQNQL"
     gene            3484..3636
                     /locus_tag="PK856_01625"
     CDS             3484..3636
                     /locus_tag="PK856_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01101.1"
                     /translation="MSIYDMRHQEQLDDDVENQVAYDDQGRSSMRQLVLCLALAVPFM
                     IILVLW"
     gene            4117..4770
                     /locus_tag="PK856_01630"
     CDS             4117..4770
                     /locus_tag="PK856_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01102.1"
                     /translation="MKMWMVAVCVVLGLAACGTSGGGLRNDAYKLITEKREEVAAYFN
                     AEGAVANAKVANGYYRKLLGKTADGSFVAQDFYSRNDVKQSDPFIISNESGLKAFTNQ
                     YTHGELFLYYANGAKMEHSVYEKGQLVSTHTAYYPSGTVFQVNQFENGKLNGRSQFYH
                     PDGKLAANLEYVDGALVNAEGWSTDGQSVSASNANSLLQQLQGYDDANMQELRRLAQ"
     gene            complement(4832..6364)
                     /locus_tag="PK856_01635"
     CDS             complement(4832..6364)
                     /locus_tag="PK856_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HRL01103.1"
                     /translation="MLRLAILGFCLVLSACATLPPAEPVTEPVVRITDTNNELAKVVS
                     TLRTNRDNLTGIYPLSDSHDSFAARASLIQAAEHTLDLQYYIWHNDASGQLLARALMQ
                     AADRGVKVRLLLDDNNTNGLDALMLRLDAHPNIQVRLFNPFVNRKFRFVGYLTEFKRL
                     NHRMHNKAFIVDQQAAILGGRNIGDEYFGNGDGVMFMDLDVLAVGEVTEDVSADFERY
                     WRSPSSYALAQILGEKKVYTPKEKLKPEERAKRQEDRQTYLQKIQESDFANELQEQRL
                     PLMWSKAKLVSDDPAKALGNSRKTDRHIVDDLSQELGEPKQSFLIVSPYFVPTKTGTH
                     ELIQLANSGIDITILTNSLAATDVAPVHSGYAKYRKPLLRAGINLYELKPTVIKPVIF
                     KDRGLTGSSGSSLHAKTIAVDNETLFIGSFNMDPRSVNLNTENGVVIHNPELTRHFKQ
                     TLLSNVPLNAYEVQLDEQQRLVWLEHSDNGVTVYHHEPKAGWFKRAMVRFLSWFPLEP
                     LL"
     gene            complement(6792..9386)
                     /gene="leuS"
                     /locus_tag="PK856_01640"
     CDS             complement(6792..9386)
                     /gene="leuS"
                     /locus_tag="PK856_01640"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HRL01104.1"
                     /translation="MQEIYQPSAIEPAAQKLWDDARLFNVEADETKPKFYSLSMFPYP
                     SGKLHMGHVRNYTITDVLSRYKLLNGYNVMQPMGWDAFGLPAENAAIKNQVAPAAWTY
                     SNIDYMKTQLQSLGFAIDWERELATCTPEYYKWEQWFFTKLFEKGIVYKKNGVVNWDP
                     VDQTVLANEQVENGRGWRSGALIEKREIPMYYFKITDYAEELLQDLDKLEHWPEQVKT
                     MQRNWIGKSRGMQVRFTVAEDSKTDLPEGWNEYLQVYTTRPDTLMGATYVAVAAEHPL
                     ATAAAKDNAELQAFIAECKAGSVAEADMATMEKKGLPTGRFVINPLNGDVLPVWVANY
                     VLFSYGDGAVMAVPAHDERDFEFAHKYDLPIKTVISPSEGEALAEPLQAAYTEHGVLV
                     NSGEFDGLDFQAAFDAMAAKLEGLNAGAPKTQFRLRDWGISRQRYWGCPIPIIHCACC
                     GDVPVPEDQLPVVLPENVVPDGAGSPLNKMPEFYETTCPKCGGAARRETDTMDTFMES
                     SWYFARYASPKCDTAMVDPAAAKYWMAVDQYVGGIEHAILHLLYARFFTKLMRDEGLL
                     AVDEPFTRLLTHRINANGTKEYYSAEELDLKRTDKGHITATLKADGEPVEYGGFLKMS
                     KSYKNGVDPQALINEYGADTARLFMMFAAPPELSLEWSEHGVKGAYNFLRRLWRTVYD
                     FQQNGLSKAFTGSQEGLDAGLKDLRYKLHTTIAKVSDDYGRRQQFNTAIAAVMELMNT
                     FDKTDTSSEQGRAVAQEVLEAITRLLWPIAPHACQALWQILRPDTLLWDAAWPQVDET
                     ALVKSELEVMVQVNGKLRGKITVAADASKEALEAAAMANEGAIKFMEGKAPKKVIVVP
                     GRLVNIVV"
BASE COUNT         2525 a         2099 c         2410 g         2547 t
ORIGIN      
        1 tggcggagag gaagggattc gaaccctcga tacgctataa acgtatacac gctttccagg
       61 cgtgcgactt caaccactca tccacctctc cgaaagaaag cgcattatac cgataatttg
      121 gatttatgca aaataccgat aaggagtgtt aatcgtttag cgttattaag atagggcgaa
      181 actttttagc catgacagaa tcctatcgtg gtattgagca aattgtgtaa cagtgtcact
      241 attgcgttgt gaattatgtg ttttacataa ccattacaag gaatttaaaa atgaaaatgt
      301 ggaaaacctt cagtatcgtt gcagtgtgtt caactttggc cttaagcgcg tgtgtgactg
      361 atcccgtgac ggggcaaacc aaccgtaata ataccgtgat tggtgcattg ggtggtgcgg
      421 cgacttgcgg tatcgtgggt gctttgactc atggcagcaa aggtgcgcgt aactctgcat
      481 tggcttgtgg tgcgattggt gcgggtgcag gtgcttacat ggattaccaa gagcgtcaat
      541 tgcgtcaaag tttggccaat acccaagttc aagtggatcg tgtgggcgat gaaatccgtt
      601 tggtcatgcc tgaaagcatt acatttgcca ctggcagcgc tgctttgaac agctcgtcta
      661 catctacctt gacttcagtt gccgatgttt tggccaaata caccgatacc acgattcaag
      721 taatcggtca cactgacagc actggttctg atgccatcaa tgaacctttg tcacgcaacc
      781 gcgcggcagc tgttgctaat ttcttgagca accgtggcgt ggcggctaac cgcatttctg
      841 caactggtat gggttcacgt caacctatcg ccagcaacac cactgccgat ggtaaagcac
      901 aaaaccgtcg cgtagaaatc aaagtaaacc cgattcaacg ctaatgcatt gaatgagata
      961 ttaagcggcc tgtaaatatt tacaggccgc ttttttatgg attaagagat tatgaggttt
     1021 gaagtaggtg ttgagcgata cgaagtgttc tacataatgc cattcacact caagagtgga
     1081 gtgtacatac ctatcattca cacaagcaaa tacatcaagc ggcctgtaca tgcccttaag
     1141 cctcttgctg tggggactta atcaaattgg attgttgaac aaagtcagcc aaagtcatcg
     1201 aaaaagtgga gaggctgggc tgcataaaat tttgtaccat caaaccaatg tggtcgtctt
     1261 gattgctttt gacccgatac acaaaaattt caaacacatc gcgcaaattg atttcgtatg
     1321 ggttggtttt taagagccag ccatcgttat cgcgggtgac aaagtggtag tgttgcagtt
     1381 gttccagtaa ttcgcccaat tcatcgtagc ccatattaat atggctgcga aatgcgcgcg
     1441 tgggcaaact gctgccttgg cattgcgctt gcgccagtaa aatcaaaatt ttcaacacat
     1501 catcaaaacg accatgctgc cccgcgtggc gttgaaaggc atcgccttgc caataagata
     1561 aggcattggt caagatggct cccgataata agattgccca caagacattg atccacaaga
     1621 ggaacagcgg tattgcggca aacgccccgt atatgacttc atagctgcca aaatggctga
     1681 aataatagct gaagatgcgc ttggccaatt ccaaaatgat cgcactccac aaagcccctg
     1741 ccagtgcatg tttcgatggc acatagcgcg caggcaccac gcggtacatc agccacagta
     1801 aagcggtgtt gaccaaggtg gaaatcacaa aatgcaatat ccatgcggtg tttggatatt
     1861 gaaatgcaaa taccgattga cgcatgaacc agccaatttg tgaaatggtc aatcccaaaa
     1921 tcaatgggcc taaagtcaat aaggcccagt acaccaacaa gcgggttagc aaagggcggg
     1981 gttgattggc tccccaaatt tggttgaatg tggtttcgat ggtgttgatt aacagcaccg
     2041 ccgacacgat gagcataatc aagcctatgg tggtgagctt ggaggcattg tccagaaagc
     2101 cattgttgaa attggcgggt gatgtcttca aacatcggaa aagctgatac cacagtcaag
     2161 gctatggtga aaaaagggac caatgccaat aatgtagtga aggtgaggct agacgagact
     2221 tgaaacaggc gcaattccaa acagcgcttc aaaacgaaca caccaaattg ccataagcgc
     2281 tgttgcttga ggcgcatgaa gagggtattc atcccagcaa tgttcataag gcgtcctgta
     2341 aatacacatg gctgttcatt gtagcgtatt atctgccttg aattgaaatt gtcagaaagt
     2401 gaacgatgct gaaaatattg gttttgtact acagtcaaaa tggcagtgtg ttgaatttgg
     2461 cacgagaaat cgccaaaggc atagacagta tggcaggttg tgaggcagtg ttacgcaccg
     2521 tgcccaaggt gtctactgtg tgtgaacagg tggcagacag cattcctgac agtggtgcac
     2581 cgtatgtcag cattgaagat ttgcgtacct gtgcaggttt ggccatgggc agtcccactc
     2641 gttttggcaa tatggcggcg gcaatgaagt attttttaga tggaaccagt ggggtgtggc
     2701 tgtcaggaga cttaattggc aagcctgcca gcgtgtttac cagcagcagc agtatgcatg
     2761 gaggacaaga gtccacatta ttgaccatga tggtgccatt gttgcatcaa ggcatgatnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnngtgtgc cttacagtga agccgatgtg ggcagcacca
     2881 agactggggg aaccccgtat ggcgcgtcgc atgtttctgg cagcgagggg aaaaatcaat
     2941 tgagccctgc tgaaaaacgc attgcctttg cacaaggtaa gcgtttggcg caaacggcgt
     3001 tgcaactttc gttagggcaa aatcagcttt gagcacttga tgcaagaatg aaaatgtcag
     3061 ttttgacaaa acagacgcaa ttggtcagtt gttgtgttga aaatgcacag gcactgagtt
     3121 taagcaagtg tttaacatga ttcatttttg atggccacgg tctttttagg gactgtggct
     3181 tttttatgca gtgaaatgtt gttgaatgat ttgtgttaag ttatggatta ttttgaatgt
     3241 ttagtgcatt tttataataa aaatgcaaaa acagtgcgta aaaaaaggaa tatttgatta
     3301 tgtggcatat ttattgcagt aatgcaaata ctcagtcact taagtcagag attaagaatc
     3361 aagagcaaaa cagcggaaca attctttgga ttacagtggc gctgattttt aataaggaca
     3421 aataagatgt taagcaattt gttcttggtt tttatattgt taatggctgg gttgttggtg
     3481 ggtatgagca tttatgacat gcgtcaccaa gaacagctgg atgatgatgt ggaaaaccaa
     3541 gtggcatatg atgatcaagg cagaagctca atgcgccaat tggtgttgtg tttagcttta
     3601 gcggtaccat tcatgattat tttggtattg tggtagtgtg cctactttgt ctttttcagt
     3661 acattgaatg tcatcatgtc atttaattgt agtggattga atgatgttgc gcttatgatt
     3721 tatcaaaaat gaaacccttg gttgggttcc tgtcagtgaa gtgttcaaaa taccgttttg
     3781 gtttaagtct ctcgctttta aaatcccgca aacttagcta tctgtgattt cacggtgtta
     3841 aatcacaggc tgtttgtata gagccatccg attatagaaa caaataattc atggtgcggg
     3901 ctttctctct caaaactaag aatagacacc aatagcctgt cgatgcttgg gtcttgaata
     3961 tttgggttta tctttagatc atggttaggc gcgagacaag tcaacgctta ttggctgaac
     4021 atttaccgtc acttacacct caaaatggta tcatgcctgg tcaaaaatcc atcgccaagt
     4081 gtgatgagat ggctaatcag caagaggatg attgcaatga aaatgtggat ggtggcagtg
     4141 tgtgtggtct tgggcttggc ggcatgtggt accagtggtg gtggcttgcg caacgatgct
     4201 tacaaactca tcactgaaaa aagagaagaa gtggcggcgt atttcaatgc ggaaggagcg
     4261 gtggccaatg ccaaagtggc caatggttac taccgcaagt tgttgggtaa gacggctgat
     4321 ggcagctttg ttgcacaaga cttttacagt cgcaatgatg tgaaacaaag tgacccattc
     4381 atcatcagca atgaaagcgg cctgaaagca tttaccaatc aatacacgca tggcgagttg
     4441 ttcttgtatt atgccaatgg tgccaaaatg gaacacagtg tgtatgaaaa aggccaattg
     4501 gtcagtaccc acacggcgta ttatccaagc ggcacagtgt ttcaagtcaa ccagtttgaa
     4561 aatggcaaac tcaatggccg ctcccagttt taccatcctg atggcaaatt ggccgccaat
     4621 ttggaatatg tggatggtgc gttggtcaat gccgagggct ggagtaccga tggtcaatcg
     4681 gtttctgcga gcaatgccaa cagtttgttg cagcaattgc agggctatga tgatgccaat
     4741 atgcaagagt taagacgatt ggctcagtaa ttcaatataa aacaaagcgg cctgtaaatc
     4801 ttttacaggc cgctttgttt atcacttgta tctataacaa tggttctaac ggaaaccaag
     4861 acaaaaatct caccatggca cgcttgaacc aaccggcttt cggttcatgg tggtaaaccg
     4921 tcacaccatt gtcgctgtgt tctagccata ccagacgttg ctgctcatcg agctgtactt
     4981 cgtatgcatt gaggggcaca ttgctgagca aggtttgttt aaagtggcgg gtgagttcag
     5041 ggttgtgtat gaccacacca ttttcagtgt tcaaattgac tgagcgggga tccatattga
     5101 aagagcctat gaacagggtt tcattgtcta ccgcaatggt tttggcatgc aagctagagc
     5161 ctgagctgcc cgttaagcca cggtctttga agatgacggg cttgatgaca gtgggcttga
     5221 gttcatacaa attgatgcct gctcgcaata agggtttgcg gtatttggcg taaccagaat
     5281 gaacgggtgc cacatcggtg gcggccagcg agttggtgag tatggtgatg tctatgccgc
     5341 tgttggccaa ctgaatcaat tcatgggtgc cagttttggt gggcacaaaa tatggagaca
     5401 cgatcaaaaa gctttgtttg ggttcgccca actcttgcga taagtcatcc acaatgtggc
     5461 ggtctgtttt gcggctgttg cccaaggctt tggctgggtc gtctgacacc agttttgctt
     5521 tagaccacat cagcggcaag cgctgttctt gcaactcatt ggcaaagtcg gattcttgga
     5581 ttttctgtaa ataggtttgt ctgtcttctt ggcgtttggc ccgctcttct ggttttaatt
     5641 tttcttttgg cgtgtatacc tttttttcgc ccaaaatttg tgccaatgcg tagctagaag
     5701 ggctgcgcca atagcgttca aaatcagcgg acacatcttc tgtgacttcc ccaaccgcca
     5761 gtacgtccaa atccatgaac atcacgccat cgccattgcc aaaatactca tcgccaatat
     5821 tgcgtccgcc taaaatggcg gcctgttggt caacaataaa agccttgttg tgcatgcggt
     5881 ggttcaggcg tttgaattcg gtcaaatagc caacaaagcg aaattttcgg ttcacaaagg
     5941 gattgaacag gcgcacttgt atgttgggat gggcatccaa gcgcaacatc aaagcatcaa
     6001 gaccgttggt gttattgtcg tccaacagca aacgaacctt taccccacgg tcagccgctt
     6061 gcatcaaagc acgagccagc aattgccctg aggcatcgtt gtgccaaatg tagtattgca
     6121 aatccaaggt gtgctcagcg gcctgtatca aactggcacg tgcggcaaac gaatcatggc
     6181 tatcggagag tgggtaaatg cccgtgaggt tgtcgcgatt ggtacgcaag gtggaaacca
     6241 ctttggccaa ttcattgttg gtgtcggtga tgcgcaccac aggctcggta acaggttcgg
     6301 caggggggag cgtggcgcaa gcactgagca caaggcagaa acccaagatt gccaagcgca
     6361 acatggaatt tttgcttaaa gcaggaaaag acaagcacag tgacttgaac aacacaaaca
     6421 tggatgaacc ttagcgtgaa tgaaatgtgt gggtgtgtgg ttattgggag caaatgccaa
     6481 gccaaaatcc agctgcaaaa atgggtgtac tggagcttgt aatttaaaga aaccaagacc
     6541 gctagcatga taagggtttg ccagcattaa aaccacccag tgttgctgaa aagccaagtt
     6601 atcgcgttca ggacaaagtt gtgttgtgtt tgcaaacagg cattttgata aaagatagaa
     6661 tatataaaat attgtttgtc atcattggca gagacatatg cgtgcaaggc aaaaagagtg
     6721 attgctatga ctgcatataa caagcggcct gtaaagcttt acaggccgct tcatgctaca
     6781 aaacttcaga cttaaacgac gatattcacc aaacgacctg gtaccacaat cactttcttg
     6841 ggcgctttgc cttccatgaa tttgatggcg ccttcattgg ccatggctgc tgcttcgagt
     6901 gcttctttgc tcgcatcggc ggcaacggtg attttgccac gcaatttgcc attcacttgt
     6961 accatcactt ccaactcaga tttcaccagt gcggtttcgt ctacttgtgg ccaagccgca
     7021 tcccataata aagtgtctgg gcgtaagatt tgccacaaag cttggcaagc gtgtggcgca
     7081 atcggccaca ataagcgggt aatggcttcc aacacttctt gagccaccgc acggccttgt
     7141 tcgctgctgg tgtcggtttt gtcaaaggtg ttcatcaatt ccatcaccgc ggcaatagca
     7201 gtgttgaatt gttggcggcg accgtagtca tcgctgactt tggcgatggt ggtgtgcaat
     7261 ttgtagcgca agtctttcag gccggcatcc aagccttctt ggctgcctgt aaatgctttg
     7321 ctcaagccgt tttgttggaa gtcatacacc gtgcgccaca agcggcgtaa gaagttatac
     7381 gcacccttca caccatgctc agaccattcc aatgacagct ctggaggagc ggcaaacatc
     7441 atgaacaagc gtgcggtgtc tgcgccatac tcgttaatca aagcttgtgg atcaacgcca
     7501 tttttatacg attttgacat cttcaagaag ccaccgtatt ccacaggttc gccatcggct
     7561 ttcaatgtgg cggtgatatg gcctttgtcg gtacgtttga ggtctagctc ttcggcagag
     7621 taatactctt tggtgccatt ggcattgatg cggtgggtca gcaagcgggt gaatggctca
     7681 tccacagcta gcaagccttc gtcacgcatc aacttggtga agaagcgtgc atacagcaag
     7741 tgcaaaatcg catgctcaat gccgcccacg tattggtcga ccgccatcca gtatttggcc
     7801 gcagcagggt ctaccatggc cgtgtcgcat ttaggcgagg catagcgggc aaaataccag
     7861 cttgattcca tgaaagtgtc catggtgtcg gtttcgcgtc gcgctgcacc accgcatttc
     7921 gggcaagtgg tttcgtaaaa ctcaggcatt ttgtttaatg gcgaaccagc gccatcgggt
     7981 accacgtttt caggcaagac cacaggcaat tggtcttctg gcactggcac gtcaccacaa
     8041 caggcacagt ggataatcgg aatagggcag ccccaatagc gttggcggct gatgccccag
     8101 tcgcgcaagc ggaattgggt ttttggcgca ccagcgttta agccttccaa tttggcggcc
     8161 atcgcatcaa atgcggcttg gaaatccaaa ccatcaaatt caccgctgtt caccaacacg
     8221 ccatgctcgg tatatgcggc ctgtaaaggc tctgccaagg cttcaccttc gcttggactg
     8281 atcacggttt taatcggcaa atcgtatttg tgggcaaatt caaaatcgcg ctcatcgtgg
     8341 gcaggcacag ccattaccgc gccatcgcca tagctgaaca acacataatt cgccacccac
     8401 acaggcaaca cgtcgccatt gagtggattg atgacaaaac ggcctgtagg caagcctttt
     8461 ttctccatcg tggccatgtc ggcttcggcc actgagcctg ctttacattc ggcaataaaa
     8521 gcttgcaatt cggcattgtc tttggcagcg gccgttgcca atgggtgctc ggcggccacg
     8581 gccacataag ttgcacccat caatgtgtct ggacgggtgg tgtagacttg caaatactcg
     8641 ttccagcctt ctggcaaatc ggttttgctg tcttcggcaa cggtaaagcg cacttgcatg
     8701 ccgcgcgatt tgcctatcca gttgcgttgc atggttttga cttgttctgg ccaatgttcc
     8761 aatttgtcca agtcttgcaa caattcttca gcgtaatcgg tgattttgaa gtaatacatt
     8821 ggaatttcgc gtttttcaat caacgcgccc gaacgccaac cacggccgtt ttccacttgt
     8881 tcattggcaa gcacggtttg atcgactggg tcccagttca ccacgccgtt tttcttgtac
     8941 acaatgcctt tttcaaacag cttggtgaag aaccactgtt cccatttgta atattcaggt
     9001 gtgcacgtgg ccagttcgcg ctcccaatca atggcaaaac ccaaagattg caattgcgtt
     9061 ttcatataat caatattgga atacgtccat gccgcaggtg ccacttggtt ttttattgcc
     9121 gcattttcag caggcaaacc aaacgcatcc caacccatgg gctgcatcac attgtagcca
     9181 ttgagcaatt tgtagcgcga caacacgtcg gtgatggtgt aattgcggac atggcccata
     9241 tgcaatttgc cagatgggta agggaacatc gacaagctgt agaattttgg tttggtttcg
     9301 tcggcttcga cattgaacaa gcgggcgtca tcccacaatt tttgcgcggc aggttcgatt
     9361 gccgatggtt ggtagatttc ttgcatggtg ttgtgcgttc gttgagagtc aaacaaagca
     9421 agaagtataa cagtttcagg gggctagggg gctggtatcg gggcagaata tcgtcatgga
     9481 attgcggaca aaaaaaacca cctacgccaa gggagaaagc agcaggtggt caattcattg
     9541 cggggaaaac gtcttactca cttcagtcac caacttcgga gcaaagaggt gatgtaagcc
     9601 atcttac
//
LOCUS       DAOMVK010000023         9330 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_1862_length_9330_cov_3.347062, whole genome shotgun sequence.
ACCESSION   DAOMVK010000023 DAOMVK010000000
VERSION     DAOMVK010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 9330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9330
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1862_length_9330_cov_3.347062"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..614
                     /locus_tag="PK856_01645"
     CDS             3..614
                     /locus_tag="PK856_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HRL01105.1"
                     /translation="MKKALTAALIGLSLTQLVSGCAALAVGGIAAGGLSALDRRSTGA
                     QADDQMMELQVSNTVNAYLKEQSGKDVYVNVKTVSYNRNILLMGVVPNQDAKDFAERV
                     ARSQTAVNNVYNHITIGQARDINAAQDTWLTSKIRTMLLKPQGYNPNHVKITTFNGVT
                     YAQGILTPDEQAAVSAQISTTLGVQKVVTLYETFVPTTSPASP"
     gene            733..1248
                     /locus_tag="PK856_01650"
     CDS             733..1248
                     /locus_tag="PK856_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01106.1"
                     /translation="MENSTTAIASKPKPRRSRISKAFWGLIGLMAVVLIGLISMAVVS
                     MNKYTYATENTQKPKDPDVEIMSLGGGEGKVIAGNTLPANPIGVDESVVDSGTSGKTA
                     ETAVEQKAVGTALVDKVAVKPVTAPKKQQVAVAAVNEDKPKSKANASANKQEKVTVQH
                     KESNKQMDNLF"
     gene            complement(1277..3028)
                     /gene="ptsP"
                     /locus_tag="PK856_01655"
     CDS             complement(1277..3028)
                     /gene="ptsP"
                     /locus_tag="PK856_01655"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003747937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HRL01107.1"
                     /translation="MSIVLHGIAVGGGIAIGRALLLQQGLDNIPHYAIEASEVKEECL
                     RFQTAVKLTRRQLEQLRNTIPENAPTELGAFISLHLMLLTDVTLSREPIDIIEEKEIN
                     AEWALQVQLERLSQQFDVIEDEYLRERKQDMLQVGERIFKNLRGHEIELNVNSDLFDD
                     TILIAHDISPADTIFFKDNRVAAFVTDVGGPTSHTAILARSLDIPSVIALHNARQIIQ
                     DDEWIIVDGIDGVLIIDPDEKILAEYRKRAKIYRNQKRQLNKLKNTAATTADDVNIQI
                     FANIESPNDITSAHKFSIDGVGLFRSEFLYLNRDTLPTEDEQYEIYLDAVKRMKGKPL
                     TLRTVDLGVDKNPRWFSHGDALNPALGLTGIRLCLAEPIMFRTQMRAVLRAAVHGPIR
                     MLWPMIGSLSELRQCHAHLELAKQQLSDRGEAFQHDIPVGCMIEVPSAALMVTSLLRQ
                     VDFLSVGTNDLIQYTLAVDRSDDAVSYLYQPAHPAVLKLLTHILRSAIKAGKPVSVCG
                     EMAGDDRYTRLLLGMGLRQFSMHPSNILRVKNMIVHSNTDEVEKQVWRILRNDDPNKV
                     DDLLKKLNQLETQKTAA"
     gene            complement(3042..3311)
                     /locus_tag="PK856_01660"
     CDS             complement(3042..3311)
                     /locus_tag="PK856_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HRL01108.1"
                     /translation="MISADVTIENKLGLHARASSKFTQTAAQFQSEIWVSRKDRRVNG
                     KSIMGLMMLAAAQGTVITLEVDGLDEEAAMTALKKLIADYFGEEE"
     gene            complement(3308..3724)
                     /locus_tag="PK856_01665"
     CDS             complement(3308..3724)
                     /locus_tag="PK856_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HRL01109.1"
                     /translation="MIGLLIVTHETLGHAFSSLVSHFFGPCPDNVRLLNVAKDDTPDL
                     ILERASALIHEVNQGQGVLILTDIFGATPCNISRKLIQSDDVMMLTGLNAPMMVKAIQ
                     YSALRNDLNSLTEEVKEAALHGIMALTYEPEEATRT"
     gene            complement(3783..4613)
                     /gene="metF"
                     /locus_tag="PK856_01670"
     CDS             complement(3783..4613)
                     /gene="metF"
                     /locus_tag="PK856_01670"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003785451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HRL01110.1"
                     /translation="MNRTPLSFEFFPTRTEEGRNKVAITRQQLRQFNPEFFSCTFGAG
                     GTTRDGTLQTVLDIMAEDTPAAPHLSCVGSSSEEIADILNTYRSHGIKHIVALRGDIP
                     SGMGMMDGFRYANELVTFIREQHGDWFHIEVAAYPEYHPQAKSAQADIDFFVQKCHAG
                     ANSAITQYFYNIDAYFAFVDAVRAKGIDIPIVPGIMPIASFSNLCRFSDMCGAEVPRW
                     LRLKLQAYGDDTASIKALGLDVVTDLCDRLLSQGAPSLHFYTLNQAGLVSTVCQRLGY
                     "
     gene            complement(4898..5566)
                     /locus_tag="PK856_01675"
     CDS             complement(4898..5566)
                     /locus_tag="PK856_01675"
                     /inference="COORDINATES: protein motif:HMM:NF040019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6198 family protein"
                     /protein_id="HRL01111.1"
                     /translation="MKTIYKRLILYVTGLFVLALGCSLSIMANLGVSPVSSLAYSIHL
                     MTPWSMGLTSIVVFAAYIVIQMILNRRVDLREACLQMILSVIFGFFMDATLWMMAFLP
                     VADNWWLQAAYLFISLWVISCGLIAYFTAKLPLLPYDALSFAIKNRFDIAFSRAKISG
                     DVINVLISAILCWAVLHQWGAIGIGTAVAAISIGKILGFLNPKIQPLLMSWLHPRSQQ
                     QNAH"
     gene            complement(5780..6022)
                     /locus_tag="PK856_01680"
     CDS             complement(5780..6022)
                     /locus_tag="PK856_01680"
                     /inference="COORDINATES: protein motif:HMM:NF028342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HRL01112.1"
                     /translation="MIFIFGISPKQTALQTGEFHCPICQTTRTYQLRSQRSCVSVFFL
                     PLFSVGKEQRILHCTHCHTVLPEKFLPNSKSLQDSN"
     gene            6121..6981
                     /gene="purU"
                     /locus_tag="PK856_01685"
     CDS             6121..6981
                     /gene="purU"
                     /locus_tag="PK856_01685"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HRL01113.1"
                     /translation="MSSEHTARLLIHCQDQAGIVQAVSGFLYHSGANITSLDQYTTEA
                     VGGDYFMRVEFVLPQLTTQLNSLSDNFTRNVASRYQMNWRFSLVQERKKIALLVSRHD
                     HALMELLWRHARGQLPCDIGCVISNHEDLRHSVEHFDIPFHVVNVQADTKAVAEAQIN
                     EWVADCDVLVLARYMQILSAEFTEQWAMKIINIHHSFLPAFVGADPYRQAYEKGVKLI
                     GATAHYVTADLDQGPIIEQDVKRVDHRYTVTELRQLGAEVERQVLARAVLWHVEDRII
                     VFGNKTVVFE"
     gene            complement(7061..7420)
                     /locus_tag="PK856_01690"
     CDS             complement(7061..7420)
                     /locus_tag="PK856_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HRL01114.1"
                     /translation="MVRYALLMAGFLSLALGTIGIFLPLLPTTPFVLLAAVCFAKASP
                     RFHNWLLQHRTFGPIVSAWQQHRAVPLPAKILSSTMMALSTWMVYYRFPEYVWVWSAC
                     GITCVCVAIWIWRLPNH"
     gene            complement(7422..7982)
                     /locus_tag="PK856_01695"
     CDS             complement(7422..7982)
                     /locus_tag="PK856_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HRL01115.1"
                     /translation="MTDTDLNHIAEETLHWLQTAVIGLNLCPFAKAVFNKGLIRIEVS
                     HARHLDDFLEHLDRELLLLRDTPADEIETTLLVEPQLFANFELFNDVLDITDQVLVEH
                     GLEGVIQIAPFHPQFQFADSEPNAISNYTNRSPYPTLHLIREDSIAKAMQAFPDADTI
                     FERNIALLEQMGIEGWQALWKQDKAS"
     gene            complement(7975..8457)
                     /locus_tag="PK856_01700"
     CDS             complement(7975..8457)
                     /locus_tag="PK856_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRL01116.1"
                     /translation="MQNIVLDKIDLKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGVIQRYAALLSPAAIELGLQAFIRVTLNKAGGGREKFDEAVQGWQEILSCFAL
                     TGETDYMLHAFFVDMEHFSHFVLEVLLNQEGVQDARSSFVLREVKKTTALPLNHLHND
                     "
     regulatory      8785..8882
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      8911..9004
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            9053..>9330
                     /gene="gcvT"
                     /locus_tag="PK856_01705"
     CDS             9053..>9330
                     /gene="gcvT"
                     /locus_tag="PK856_01705"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="catalyzes the transfer of a methylene carbon from
                     the methylamine-loaded GcvH protein to tetrahydrofolate,
                     causing the release of ammonia and the generation of
                     reduced GcvH protein; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRL01117.1"
                     /translation="MSEPQKTPFYNAHVAADGKMVDFSGWDLPIHYGSQIKEHEFVRT
                     DAGMFDVSHMVVTDVSGEKAKAWLQYLLANDVDKLKFVGKALYSPMLN"
BASE COUNT         2542 a         2041 c         2339 g         2408 t
ORIGIN      
        1 gcatgaaaaa agcattaaca gcagcattaa tcggtttgag tttaacgcaa ttggtaagtg
       61 gctgtgccgc attggccgtt ggcggcattg ccgcaggtgg attgtctgcg ctagaccgcc
      121 gcagtacggg ggcgcaagca gatgatcaaa tgatggaatt gcaagtaagc aataccgtta
      181 atgcctattt gaaagaacaa agtggcaaag atgtgtatgt taatgtgaaa accgtcagtt
      241 acaaccgcaa tatcctgttg atgggtgtgg tgccgaatca agatgccaaa gattttgctg
      301 agcgagtagc gcgttcacag acggcagtta acaatgtgta taaccacatc accatcggtc
      361 aggcacgtga catcaatgct gcgcaagaca catggttgac atctaaaatt cgtaccatgt
      421 tattaaagcc tcaaggctat aatcccaacc atgtgaaaat caccactttc aacggggtga
      481 cttacgctca agggatactt accccagatg agcaagccgc agtcagtgcg caaatcagca
      541 cgactttggg cgtacaaaaa gtcgtgacct tatacgagac ctttgtgcct acgaccagtc
      601 ctgcttcacc ataagtctca ttattcggcc gcgttgcggt cgaatgcaga cgttctaaag
      661 tatttgtgtt aaagtgtcga cgcaatttcg ttaaattaat cagagtcata ggacactttg
      721 gatttgaaaa tcatggaaaa tagtactact gcaatagcaa gcaaacccaa gccgcgtcgt
      781 tctcgcatca gcaaagcttt ttggggtttg attggtttga tggcggttgt tttgatcgga
      841 ctcatcagta tggctgttgt ttctatgaac aaatacactt atgccaccga aaacacgcaa
      901 aagcctaaag atcctgatgt ggaaatcatg agcttaggtg gcggagaagg caaagtgatt
      961 gcaggcaata ctttgccagc caatccgata ggtgtggatg agtcggttgt agacagtggc
     1021 acttctggta aaaccgctga aaccgctgtt gagcaaaaag ctgtgggcac ggctttggtg
     1081 gataaggtcg ccgtaaaacc tgtcactgct cctaaaaaac agcaagtggc agtggctgcg
     1141 gtgaacgaag ataagcctaa atcaaaagcc aatgcttctg cgaacaagca agaaaaagtc
     1201 actgttcagc acaaagagtc caacaaacaa atggacaatt tgttttaatg cggcctgtaa
     1261 ataaaaaacc gctgccttag gcagcggttt tttgtgtttc taactgattg agttttttca
     1321 ataaatcatc gactttgtta gggtcatcat tgcgcaagat gcgccaaact tgtttttcaa
     1381 cctcatcagt attgctgtgt acgatcatgt ttttcacccg caaaatattt gatgggtgca
     1441 tggaaaattg gcgcaaaccc atgcccagta ataaacgggt atagcgatca tcgcctgcca
     1501 tttcaccaca caccgatact ggtttacctg ctttgatggc gctgcgcaaa atgtgcgtta
     1561 acagctttaa caccgcagga tgagcgggtt ggtacaaata gctgacggca tcgtcactgc
     1621 ggtctaccgc cagtgtgtat tgaatcaaat cattggtgcc tactgacaaa aagtcaactt
     1681 ggcgcaataa gctggtgacc atcaaagccg ctgatggcac ttcaatcatg catcctacag
     1741 gaatatcgtg ttggaatgct tcgccacggt ctgacagttg ttgtttggcc agctccaaat
     1801 gggcatggca ttgacgcaac tcgcttaaag agccaatcat gggccacagc atgcgtattg
     1861 ggccgtgtac cgccgcgcgc aatacggcgc gcatttgggt gcggaacatg atgggttcgg
     1921 ccaagcagag gcgtatgccc gtcaaaccca aagcaggatt taaggcatca ccatggctga
     1981 accagcgcgg atttttgtcc acccccaagt ccaccgtgcg caaagtcagt ggtttgcctt
     2041 tcatgcgttt gaccgcatct aagtaaattt cgtattgctc gtcttcggtg ggtaaggtat
     2101 cgcggttcaa atacaaaaat tcgctgcgga acaagcccac gccatcgatg gagaatttgt
     2161 gagcgctggt aatgtcattg ggcgattcaa tgttggcgaa tatttggatg ttgacatcgt
     2221 ccgcagtggt ggcagcggtg tttttcagtt tgtttaactg acgtttttga ttgcggtaaa
     2281 ttttggcgcg cttgcggtat tccgccaaaa ttttctcatc tgggtcgatg atcagtacgc
     2341 catcgatgcc gtcaacaatg atccactcat catcttgtat gatttggcgg gcattgtgta
     2401 aggcgataac cgatggaatg tccaagctgc gcgccaaaat cgcggtatga ctggtggggc
     2461 cgccaacatc ggtgacaaat gccgcgacac ggttgtcttt gaagaagatg gtgtcggcag
     2521 gagagatgtc gtgggcaatc aaaatggtgt cgtcgaacaa gtcgctattc acattcaatt
     2581 caatttcatg accacgcaaa tttttaaaaa tacgctcgcc cacttgcagc atgtcttgtt
     2641 tgcgttcacg caaatactcg tcttcaatca catcaaattg ctgagaaaga cgctctaatt
     2701 gaacttgcag tgcccactcg gcattgattt ctttctcttc aatgatgtct atgggttcgc
     2761 gtgaaagcgt gacatcggtt aacaacatca aatgcaagga gatgaaagcc cccagttcgg
     2821 tgggtgcatt ctcagggatg gtgttgcgca actgttcaag ctgccgtcgt gtcagtttga
     2881 cggcggtttg aaagcgcagg cactcttctt tgacctcact cgcttcgatg gcgtaatgag
     2941 gaatgttgtc caatccctgt tgcaataaga gggcgcggcc aattgcgatg ccgccgccca
     3001 cggcgatgcc gtgtaacacg atgctcatgc tgctccttag cctattcttc ctcgccgaaa
     3061 taatcggcaa tgagtttttt caaggcagtc atggcggcct cttcatctaa gccatccact
     3121 tccaaagtaa tcactgtgcc ttgcgcggct gccagcatca tcaaacccat gatgctttta
     3181 ccgtttacac ggcggtcttt gcgcgacacc caaatttccg attgaaactg cgcggcggtt
     3241 tgggtgaatt tgctggaggc acgggcgtgt aagcccaatt tgttttctat ggttacgtct
     3301 gcgctgatca tgttcgtgtc gcttcttctg gttcgtaggt gagggccata atgccgtgta
     3361 aggcggcttc tttcacttct tcggtgagtg agttcaagtc attgcgtaat gccgagtatt
     3421 gaatggcttt taccatcatc ggagcattta agcccgtgag catcatcaca tcatcggatt
     3481 gaatcagttt gcgtgagatg ttgcacggtg tggcgccgaa gatatcggtt aatatcaaaa
     3541 ccccttgtcc ttggttgacc tcatgaatca aggctgaggc ccgttctaaa atcaaatcag
     3601 gcgtgtcatc tttggccaca tttaacagcc tgacattgtc tgggcaaggt ccaaaaaagt
     3661 ggctgaccaa tgaactgaag gcatggccta aggtttcatg ggtcacaatc aacaatccta
     3721 tcatgttgtt aactcactga agcttacagg ccgcatggta tcagcggcct gtaaatgggt
     3781 tgtcaatagc ccaatcgttg gcacacagtc gaaaccaaac ctgcttgatt caaggtataa
     3841 aagtgcaggc tgggtgcgcc ttggctgagc aagcggtcac acaaatcggt gaccacatcg
     3901 aggcccaagg ctttgatcga tgcggtgtca tcgccataag cttgtaattt caagcgcagc
     3961 caacgtggca cttcagcgcc acacatgtct gaaaatcggc acaaattact gaagctggca
     4021 atcggcataa tgcctggaac gatggggatg tctatgcctt tggcgcgaac ggcatccaca
     4081 aaggcaaaat aggcgtcaat attgtaaaaa tactgggtaa tggcggagtt ggcccctgca
     4141 tggcattttt gcacgaaaaa atcgatgtcg gcttgggcac ttttggcttg aggatggtac
     4201 tcagggtagg ccgcaacttc aatgtgaaac caatcaccgt gttgctcgcg gatgaaggta
     4261 accaattcat ttgcatagcg gaagccatcc atcatcccca tgccagaggg aatgtcgcca
     4321 cgcaaagcca caatgtgctt gatgccatgc gagcgatagg tattcaaaat gtcggcaatt
     4381 tcttctgaac tcgaacccac acaagacaaa tgcggtgcag ctggtgtgtc ttccgccatg
     4441 atgtcgagca cggtttgcaa agtgccatcc cgagtggtgc cgcctgcgcc aaacgtacaa
     4501 gagaaaaact caggattgaa ttggcgcaat tgctggcgcg taatcgcgac tttattccgt
     4561 ccttcttcgg tacgggttgg gaagaattca aagcttaaag gggtgcggtt catttgttaa
     4621 tattctggcc agaagaaagg tcttaaccat acctcagcat ggcggtttgg gcaattgtta
     4681 gcttggataa aatgcctaat ccattgcacc aagcacattg ctccatcaaa caatcgcatg
     4741 cggcctgtaa aaccaaggtt tgtcagcccg catgtgagcc atttgggtta aataaggtat
     4801 gcggtttaga ggtttcatca tacaagaaaa acacatgcgg cttgaattat tttacaggcc
     4861 gcttgtgagg tggttttggc tgttgcccag aacgggttta gtgagcgttt tgttgttggc
     4921 tgcgagggtg tagccaagac atgagcaagg gttggatttt cggatttaaa aaaccgagaa
     4981 ttttgcctat gctgatcgct gcaacggccg tgcctatgcc tatggctccc cattggtgta
     5041 agactgccca acacaaaatg gcagaaatca gcacattgat gacgtctcca ctgattttgg
     5101 cgcggctaaa agcgatgtca aagcggtttt tgatggcgaa actcaaggca tcatagggca
     5161 gaagtggcaa ttttgcggtg aagtaagcta tcaggccaca ggaaatcacc cacaaactga
     5221 tgaacaaata agcggcctgt aaccaccaat tgtctgccac aggtaagaag gccatcatcc
     5281 acaaggttgc atccataaaa aagccaaata tcacgctgag tatcatttgc aaacaggctt
     5341 cacgcaaatc aaccctgcgg ttcaaaatca tttggatgac aatgtaagcc gcaaacacca
     5401 caatactggt cagacccatg gaccatggcg tcatcaaatg gatggagtag gccaaagacg
     5461 aaacgggcga tacgccaaga ttggccataa tggagaggct gcagcccaaa gccagcacaa
     5521 acaggccagt tacataaaga atcaatcgtt tgtatatggt tttcattaat acattatatc
     5581 aatatattca cgatgaaaaa gcagtgagac tcaataaaat catttaaata atgtttaaaa
     5641 ttaaatgagt tagattattt gttggtgaaa tgacggtttg tttctgttga ttttcatgaa
     5701 tctattacat tgttaattgg gtgaacgatg tgtgtgatgg aaggaagtag attatgaggg
     5761 tgcaaccaag acgattggct taattggaat cttgcaaaga cttgctgttc ggcagaaatt
     5821 tttcgggcaa taccgtatgg cagtgtgtgc aatgcaaaat acgctgttct ttgccaacgg
     5881 aaaacagagg gaggaaaaac acagaaacgc aagagcgctg gctgcgcaat tggtaagtgc
     5941 gggtggtttg gcaaatcggg caatggaatt caccagtttg taatgctgtt tgcttggggc
     6001 tgatgccgaa aataaaaatc atgaggaaag acgcactaaa taccaatgaa gacgcgacat
     6061 tttagcctga gcatggctac aatagggtga aatgttttca tcagaaagtc actgcaaaat
     6121 atgagtagcg aacacacagc aagattgttg attcattgcc aagaccaagc cggcatcgta
     6181 caagctgttt cggggttttt gtaccacagt ggtgccaaca tcacgtcttt agaccaatac
     6241 acaaccgagg ccgtaggcgg tgactatttc atgcgcgttg aatttgtgtt gccacaattg
     6301 acgacgcagt taaacagtct gtccgataat ttcacccgca acgtcgccag tcgctatcaa
     6361 atgaactggc gcttcagttt ggtgcaagag cgcaagaaaa ttgctttgtt ggtgtccagg
     6421 cacgaccatg cgctgatgga gttgttgtgg cggcatgcgc gtgggcaatt gccatgtgat
     6481 attggctgtg tcatcagcaa tcacgaagac ttgcgccatt cggttgagca ttttgacatt
     6541 ccgtttcatg tggtgaatgt acaggccgat accaaagctg ttgctgaggc gcaaatcaat
     6601 gagtgggtgg ccgattgtga tgtgttggtg ttggcgcgct acatgcaaat tttaagtgct
     6661 gaatttaccg agcaatgggc gatgaaaatc atcaatatcc accattcttt cttgcctgca
     6721 tttgtggggg ccgatccata tcgccaagcc tatgaaaaag gcgtgaaatt gataggcgca
     6781 acagctcact atgttactgc tgatttggat caagggccca tcattgagca agatgtgaaa
     6841 cgggtagacc accgttacac tgtgactgag ctgcgccaat tgggtgcaga agttgagcgc
     6901 caagtcttgg cgcgggcagt gttgtggcac gtggaagacc gcatcattgt gtttggcaat
     6961 aaaacagtgg tgtttgaata acacatttga gtatgtgaaa aacaatggat aagcggcctg
     7021 taaacagttt acaggccgct tgtttgcatg ctggagtgac tcagtgattc ggtaagcgcc
     7081 aaatccaaat ggccacgcaa acacaggtga tgccgcaggc gctccaaacc catacatatt
     7141 cgggaaaacg gtaatacacc atccatgtgg acaaagccat catggtgctg gagaggattt
     7201 tggcgggcag tggcacggca cggtgttgtt gccatgcgct gactatcggg ccgaacgtgc
     7261 ggtgttgcaa cagccagttg tgaaaacggg gcgaggcctt ggcaaaacac actgctgcca
     7321 ataacacaaa tggcgtggtg ggcaacaggg gcagaaaaat acctatggtg cccaaggcca
     7381 gtgacaaaaa tcccgccatc aataaggcat agcgcaccat atcaagaggc cttgtcttgt
     7441 ttccataagg cttgccaacc ctctatgccc atttgttcca ataaggcaat gttgcgttca
     7501 aaaatggtat cggcatctgg gaaggcttgc atggctttgg caatgctgtc ttcgcggatt
     7561 aaatgcaaag tggggtaggg tgagcggttg gtgtaattgc tgatggcatt gggctctgaa
     7621 tcggcaaatt gaaattgtgg gtgaaacggc gcaatttgaa tgacgccttc caagccatgt
     7681 tccaccaaga cttggtcggt aatgtcgagc acgtcgttga acaattcaaa attcgcaaac
     7741 aattgcggtt ccaccaataa cgtggtttca atttcatcag cgggggtgtc gcgcaataac
     7801 agcaactcac ggtctagatg ttctaaaaaa tcatccaaat ggcgggcatg actgacttca
     7861 atgcgtatca accccttgtt gaacacggct ttggcaaacg ggcacagatt cagaccaatg
     7921 actgcggttt gcagccaatg gagggtttct tctgctatgt ggtttaaatc ggtatcagtc
     7981 attgtgcaaa tggttcaaag gcaaggcagt ggttttcttc acttcgcgca atacgaagct
     8041 agagcgagcg tcctgtacgc cttcttgatt gagcaatact tccagcacga agtgagagaa
     8101 gtgctccatg tcgacaaaaa aagcatgcaa catgtagtcg gtttctccag tgagtgcgaa
     8161 acagctcaaa atctcttgcc agccttgtac cgcttcgtcg aatttttcgc ggcctccacc
     8221 tgctttgttt aaggtcacgc gaataaacgc ctgtagaccc agttcaatgg cggcagggct
     8281 gagcaaggct gcatagcgct gaatcacacc tgcatcttcc aattgtttta agcgacgtaa
     8341 gcaaggagaa ggagacaggg cgacacgttc agacagttct acgttggtta aacgcccatt
     8401 ttcttgcaat acttgcaaga ttttcaaatc gattttgtcc aatacgatgt tttgcatatg
     8461 agtggccccc tttgtgcgcc atttttttat ttgggtacga gtagactttt gtattattta
     8521 tttgtgtgag tgttcacaaa tatcacgatt cagcgattca ggaacataat atgacaatat
     8581 tttaactcaa aagccattat tttttataaa aaattataaa aaaccaaaat tgatgcgaaa
     8641 tgttgcgatt tttaaattgt tttagtgggg ttggtttgct cgtttatcca agagcaaagt
     8701 tgcaccgtgc ttagagcaaa agtttgattt taaaataagt atgtcggcaa cccttgccaa
     8761 aagattcgat tcattcaata atggcggcgt ttagcaggag aatgacgaaa atgaaccaat
     8821 tctcgtctac cgaaggcgca tagaccctta aatcgctcag gtaacaggac tgcttaaccc
     8881 tcatcttcgc aatcatatta aataagaaga cgaggcaaat ctggagagtg gcttacgccc
     8941 accgaagggg gaaagaatga ggcaatcatt cgaaaatctc aggtatcagg acagataggg
     9001 aatggctgtt gtatggacaa caagcattct taattctatg gagtgatggc aaatgagtga
     9061 accccaaaaa accccatttt ataacgccca cgttgctgcc gatggcaaaa tggtcgattt
     9121 ctctggctgg gatttaccca tccactatgg ctcccaaatc aaagaacacg aattcgtgcg
     9181 taccgatgcc ggtatgtttg acgtgtctca catggtggta accgatgtgt ctggcgaaaa
     9241 agccaaggca tggttgcaat acctgttggc taatgatgta gacaaattga aattcgttgg
     9301 caaggctttg tattcaccga tgttgaatga
//
LOCUS       DAOMVK010000024         9326 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_1865_length_9326_cov_3.683206, whole genome shotgun sequence.
ACCESSION   DAOMVK010000024 DAOMVK010000000
VERSION     DAOMVK010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 9326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9326
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1865_length_9326_cov_3.683206"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..169)
                     /locus_tag="PK856_01710"
     CDS             complement(<1..169)
                     /locus_tag="PK856_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HRL01118.1"
                     /translation="MNETFDLQAHEHIALCDLLKITGATESGGQAKAMISDGLVWRNG
                     EVETRKTAKIKD"
     gene            437..1018
                     /locus_tag="PK856_01715"
     CDS             437..1018
                     /locus_tag="PK856_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01119.1"
                     /translation="MHSKFAPSPYLPIMNQVYYNKSMLLTALLPMLLIVVSTLYLLLD
                     GADAVRKTFLFGVLDSPWLFYPILLTICLTFVQYSYPSLRKLLRKKPAIRWGAGGVAL
                     AKDARVAWAAVEGVELVEHKKNPYILLHIDHPFAFMDMQVGERKTEAKQCYKLFNTPV
                     AIACSELAVDAHDLYLALQSQHQSFQAEQCSKP"
     gene            1003..1329
                     /locus_tag="PK856_01720"
     CDS             1003..1329
                     /locus_tag="PK856_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HRL01120.1"
                     /translation="MLKTLILFFATALMELLGCFLPYVWLRQHGSVWLLPLAAVCLIA
                     FVYLLSLHPEASGRVYAAYGGVYVVSALLWLRWVDKIALQTSDFIGAVVILLGVVCII
                     APWQQS"
     gene            complement(1427..1843)
                     /locus_tag="PK856_01725"
     CDS             complement(1427..1843)
                     /locus_tag="PK856_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01121.1"
                     /translation="MLIVLVIAIILLMGSVVLSAIVPHPITWLMTGILALAIAFIIWP
                     KYQTETAVQQVGVEVQATVSNVREWDRKIGDGQLRTQYEVTATWTHPDTGKTYEFISV
                     PYLKRPAVAIGDTVNVSIVWDTPEQYQMQDLHEDKP"
     gene            1887..2519
                     /locus_tag="PK856_01730"
     CDS             1887..2519
                     /locus_tag="PK856_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRL01122.1"
                     /translation="MSLLDDLQWRYAAKHLNGTPVPQADVDYIVEAARMAPTSSGLQP
                     FHVFVVRNADLLAQLSQCVYGNQQVKNCSPLLVFAAWDNYSEARVNEYFSRQNLERKI
                     PDSMTDGYRTRLLEHIARESEAENHHHAAKQAFLAAMCAMMAAAERRVDCTPMEGFEA
                     EKIDALLDLPAQGLRTTLLLPLGYRDENNDWLLRMKKVRREGNTFATQLD"
     gene            2702..3100
                     /locus_tag="PK856_01735"
     CDS             2702..3100
                     /locus_tag="PK856_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01123.1"
                     /translation="MNTIKFSYYGLILEVPYLGSHQCLVMNAQGDIFMFDHEPTISER
                     NASWIPLHGQSYFVSSAPEILQHVDWRSSYALVNELAAETAQDASKVNSVSLQLICSL
                     VQHQGLNDIEHHIDQCYELAVKIVHKQKSF"
     gene            3284..3577
                     /locus_tag="PK856_01740"
     CDS             3284..3577
                     /locus_tag="PK856_01740"
                     /inference="COORDINATES: protein motif:HMM:NF023148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="I78 family peptidase inhibitor"
                     /protein_id="HRL01124.1"
                     /translation="MLKYLSLAGLMALTAACAQITPPVGNIPAQPSKCVAEAAANLIG
                     SHGLDNAQIMQRTGASEVRRLHPNSPATMDYRETRITVVNDPLSKKIIRASCG"
     gene            complement(3632..4654)
                     /locus_tag="PK856_01745"
     CDS             complement(3632..4654)
                     /locus_tag="PK856_01745"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HRL01125.1"
                     /translation="MNKLLPTSTAGSLPKPSWLAEPEKLWSPWKLSEEVLLEGKQDAL
                     RVSLQEQLLAGVDIVSDGEQTRQHFVTTFIEHLSGVDFEKRETVRIRNRYDANVPTVV
                     GEVARSRPVFVEDAKFLRQLTSQPIKWALPGPMTMIDTLYDAHYQSREKLAWEFAKIL
                     NQEAKELEAAGVDIIQFDEPAFNVFFDEVNDWGVATLERAIEGLKCETAVHICYGYGI
                     KANTDWKKTLGSEWRQYEEAFPKLQQSNIDIVSLECHNSHVPMDLIELIRGKKVMVGA
                     IDVASNVIETPEEVANTLRKALQFVDADKLYPSTNCGMAPLARSVARGKLQALSAGAE
                     IVRREL"
     gene            complement(4688..5671)
                     /locus_tag="PK856_01750"
     CDS             complement(4688..5671)
                     /locus_tag="PK856_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1852 domain-containing protein"
                     /protein_id="HRL01126.1"
                     /translation="MNKEFTFEIKSICFDENYHPSDNTRITTNFANLARGSSRQENLR
                     NTLKMIDNRFNDLASWDNPKGDRYSVGIEIVSVDMKIGTASGEHSFPTIEILKTNILD
                     HQTNKRIEGIVGNNFSSYVRDYDFSVLLLEHNKNQEKFSIPANFGDLHGKLFQYFVGS
                     DVYKQHFNKPPVICLSVSSSKVYRRTENRHPVLGVEYVQDESSLTDQYFGKMGLQVRY
                     FMPANSVAPLAFYFHGDLLADYTNLELIGTISTMETFQKIYRPEIYNANAAAGKWYQP
                     SLNNQDHSLTQIVYDREERGQLAIKQGKFTEEHFIKPHQNTLEQWAANYAI"
     gene            complement(6146..6604)
                     /gene="pyrI"
                     /locus_tag="PK856_01755"
     CDS             complement(6146..6604)
                     /gene="pyrI"
                     /locus_tag="PK856_01755"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HRL01127.1"
                     /translation="MNTQQRSVEAICDGTVIDHIPAGLGLVILRQFGLNDLGERVTVG
                     FNLSSKNMGHKDIIKVENVRFDAEQANRLALFAPNATVNVIADYKVVDKLSLNLPEHV
                     KGLFRCPNDNCASHKEPVISSFKVKTANGETKLRCHYCEKTYPRLAVTEI"
     gene            complement(6601..7527)
                     /gene="pyrB"
                     /locus_tag="PK856_01760"
     CDS             complement(6601..7527)
                     /gene="pyrB"
                     /locus_tag="PK856_01760"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HRL01128.1"
                     /translation="MPNPLYRQHVISVADLSADTLTQLVKTALHLKQHPQPDLLSDQL
                     VATCFFEPSTRTRLSFETAAHRLGANVIGFADGANTSAKKGETLADTARIISGYADAI
                     VVRHNKDGAARVMAEFSSVPVVNAGDGTNQHPSQTLLDLITIFETQGRLEHLNIVLVG
                     DLKYGRTVHSLAQALSNWNNTFYFVAPPQLAMPDYITEELDEKGCAYQILDSFEEALN
                     VADIVYMTRVQRERFDEQEFLKIQGLYVLKAAMLSNVKDNMRIMHPLPRVDEIATDVD
                     ATQHAYYFEQAKNGVFARQAILSAVLNEELPA"
     gene            complement(7662..8258)
                     /locus_tag="PK856_01765"
     CDS             complement(7662..8258)
                     /locus_tag="PK856_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003822987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HRL01129.1"
                     /translation="MNLGYLCVMLALGVCAPAEAKRIAAIEAPAVQYTWATPVEQSVN
                     LFRVTPNLYRSEQLTAKDEALLQQHNIKTIINLRFFDRDDDKEELSHLKLNLVNHPLK
                     SWRIKPKEIAAVLYDIEKAQKQGGVLVHCYHGADRTGLIIGMYRIVNQGWSVEEAKRE
                     MVEGPYGFHTVWRNIPKMYNQDTVNQVRLELARLRAAK"
     gene            8373..>8660
                     /locus_tag="PK856_01770"
     CDS             8373..>8660
                     /locus_tag="PK856_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRL01130.1"
                     /translation="MTTPIRILGIDPGSRVTGFGVIDLLGRQQTYVASGCIKTPPKAA
                     LSERIAVLFEHISEVIDTYQPTVSAIEQVFVNVNPAATLMLGQARGACVAAL"
     assembly_gap    8661..8670
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8671..8917
                     /locus_tag="PK856_01775"
     CDS             <8671..8917
                     /locus_tag="PK856_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRL01131.1"
                     /translation="GKLPVHEYTALQVKQAVVGQGKAAKEQVQHMVVQMLRLNGTPSS
                     DAADALAVAITHALRNHNLAAQIQQGLSVKRGRFTTR"
     gene            8989..>9326
                     /gene="ruvA"
                     /locus_tag="PK856_01780"
     CDS             8989..>9326
                     /gene="ruvA"
                     /locus_tag="PK856_01780"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRL01132.1"
                     /translation="MISQLTGSIIDKQPPLIVIDVNGVGYEVFVSMQTFYGLPATGEK
                     VRIYTQMVVREDAHLLFGFGNTIERETFRLLIKVSGIGAKTALGILSVLSSDDLARAI
                     ADEDIKVLSSV"
BASE COUNT         2291 a         2369 c         2195 g         2461 t
ORIGIN      
        1 cgtctttgat tttggcggtt ttgcgggttt ccacctcgcc attgcgccac accaaaccat
       61 cgctgatcat ggccttggct tggccgccgc tttcggtcgc accggttatt ttcaataaat
      121 cacacaaggc gatgtgttca tgcgcttgca gatcaaaggt ttcgttcatg tttgctttct
      181 ctggctgctg caaaccccat gtttgcaaaa aaagctgttt ttcataatat gggggcaaca
      241 ggtgtttttt taaatcgact gtctgacaag catgtcgtga gtctgtgcgg atgctcaatc
      301 catgactgct tggcatgggc atcatgcccg ctcggtgaga ttttttaacc attactcagt
      361 cttttaggct attttacgcg aaaacacgac cgcttgtgct gcatcaagcc caccatcgcc
      421 gcgcttgcaa taccatatgc attcaaaatt tgcaccaagc ccctatttac ccatcatgaa
      481 ccaagtctat tacaacaaat ccatgttgct caccgcactg ctgcccatgc tgctgattgt
      541 ggtcagtacc ctgtatctgc tgttggatgg ggccgatgcc gtacgcaaaa cctttttatt
      601 tggtgttttg gattcaccat ggctgtttta cccaatattg ctcaccattt gcctcacctt
      661 tgtccaatac tcttatccgt ctttgcgcaa gctgctgcgc aaaaaacccg ccatacgctg
      721 gggcgcaggt ggggtggctt tggccaaaga tgcacgcgtg gcatgggctg ctgttgaagg
      781 agtggaattg gtagaacaca agaaaaaccc ctacatcctc ttacacatag accacccttt
      841 tgcctttatg gacatgcaag tgggcgagcg caaaaccgaa gccaaacagt gttacaaact
      901 gttcaacacc cccgtcgcca tcgcctgctc tgaactggca gtggatgcac acgatttgta
      961 tttggcctta caatcgcaac atcaaagctt tcaggccgaa caatgctcaa aaccctaatc
     1021 ctgtttttcg ccaccgccct catggaattg ttgggctgtt ttttacccta tgtgtggctg
     1081 cgccaacatg gctcggtgtg gctgttgccg ctcgcggcgg tgtgcttgat tgcatttgtg
     1141 tatttgctga gtttgcatcc cgaggcatcg ggacgggttt atgctgctta tggtggagta
     1201 tatgtggtca gcgccttgct gtggctgcgc tgggtggaca aaatcgcctt gcaaaccagt
     1261 gactttatcg gagcagtcgt gattttgctg ggcgtggtgt gcatcattgc cccttggcaa
     1321 caaagctaat ctggcattta catcaaacaa gcggtctgta aatatttaca ggccgcttgt
     1381 ttcggtatgt tacacatgat ttatggtaat actgactgga tgggcactaa ggcttgtctt
     1441 catgcaaatc ttgcatctga tattgctcag gtgtgtccca cacaatactc acatttaccg
     1501 tgtcacctat cgccaccgca ggccgtttga gatacggcac actgataaat tcataagtct
     1561 tgcccgtatc aggatgcgtc catgtggcgg tgacttcgta ttgggtacgc aattgcccat
     1621 cgcctatttt tctgtcccat tctcggacat tgctcaccgt ggcttgcacc tccacgccca
     1681 cttgttgcac cgccgtttca gtttggtatt tgggccaaat tatgaatgcg atagccaaag
     1741 ccaaaatacc cgtcatcaac caagtgatgg gatgcggtac aatagccgat aaaaccacac
     1801 tgcccatcaa caaaatgatg gcaatcacca acacaatcaa cacggctatt ccttcatgaa
     1861 gaggcggcct gaaagaccac acaagcatga gtttactgga cgatttacaa tggcgctatg
     1921 ctgccaaaca tttaaatggc acgcctgtgc cacaagcaga cgttgactac atcgtagaag
     1981 ccgcacgcat ggcgcccact tcatcgggcc ttcagccctt ccatgtgttt gtggtacgca
     2041 atgccgattt gctggcccaa ttgtcgcaat gtgtgtatgg caatcaacaa gtgaagaatt
     2101 gctcaccttt attggtgttc gccgcgtggg acaactattc cgaagcccga gtgaacgaat
     2161 atttcagccg ccaaaatttg gaacgcaaga tacccgacag catgaccgat ggctaccgta
     2221 cccgcttgtt agaacatatt gccagagaga gtgaagccga aaaccaccac catgcggcca
     2281 aacaagcctt cttggcagcg atgtgcgcga tgatggcagc ggcggaacgc cgcgtggatt
     2341 gcacaccgat ggaaggcttt gaagccgaga aaatagacgc gctgttggat ttgcccgccc
     2401 aaggcttgcg taccactttg ctgttgcctt tgggttaccg cgatgaaaac aacgattggt
     2461 tattgcgcat gaaaaaagtg cgccgtgaag gcaacacctt cgccacccaa ttggattaat
     2521 atccatcatc atgaaacagc cttgctcacc acaaggctgt cctcattcaa ttcattgatt
     2581 tataaaatat tatctcacga ttcaaatctg cgatttgctg tttacaggct gcttgttatt
     2641 gggtatttta tcctgattca atgcgtgttt tcaccattga aagcaagtat ggaggcgaat
     2701 catgaacacc attaaattca gttattatgg gttgattttg gaagtgcctt atttgggcag
     2761 ccaccagtgc ttggtgatga atgcccaagg cgatattttt atgtttgacc acgaacctac
     2821 catcagtgag cgcaatgcct cgtggatacc gttgcatgga caaagctatt ttgtcagctc
     2881 cgctcccgaa attttgcaac acgttgattg gcgttcgtct tatgcactgg tcaatgaatt
     2941 ggcggcagaa accgcacaag atgccagtaa ggtcaacagc gtgtctttgc agttgatttg
     3001 cagcttggtt caacaccaag gtctaaacga cattgaacac cacattgacc aatgctacga
     3061 attggccgtg aaaattgtgc acaagcaaaa aagcttttaa gccacagtca cgcttgtcca
     3121 atgcccattt gaaaatccgc tggtttgcag gctgtggcgg gttttggcgg cttcaaacct
     3181 gaatcgctca tgcaagactg tgcaaatctt gcggcacatt tcactcaagt gacatatctc
     3241 tcactataat gaacactcac tccacacact tggaaggacc agtatgttga aatacttgag
     3301 tttggccggc ttgatggcct taaccgcggc ctgcgcccaa attacgccgc ctgttggcaa
     3361 cattcctgct cagccaagca aatgcgttgc cgaagccgcc gctaatttaa taggcagcca
     3421 tggcttggac aacgcccaaa tcatgcaacg cacaggtgct tccgaagtgc ggcgtttaca
     3481 cccaaacagc ccagccacca tggattaccg tgaaacccgc atcaccgtgg tcaacgaccc
     3541 tttgtctaaa aaaatcattc gtgccagctg cggctaaaat gtttacaggc cgctttaagc
     3601 ggcctgtaaa ctgacggtca ctgtttcagt atcacagctc tcgtctcacg atttcagcgc
     3661 ctgcgctcaa tgcttgcagt ttgcctctgg ccactgagcg tgccaacggt gccatgccgc
     3721 aattggtcga aggatacagc ttgtccgcgt cgacaaattg caaggctttg cgcaaggtat
     3781 tggccacttc ctcgggcgtt tcaatcacat tactggccac atctatcgca cccaccatca
     3841 ctttcttgcc ccgtatcaac tcaatcaaat ccatgggcac atgcgagttg tgacattcca
     3901 aggacacgat gtcgatgttg gattgctgta attttgggaa ggcttcttca tattgtcgcc
     3961 attcagaacc cagtgttttt ttccaatcgg tgttggcttt gatgccataa ccatagcaaa
     4021 tgtgcaccgc tgtttcacat ttcagccctt caatcgcgcg ctccaatgtg gcaacgcccc
     4081 aatcattcac ttcatcaaaa aacacattga atgcaggctc atcaaattga atgatgtcta
     4141 cgcctgccgc ctccaattct ttggcttctt gattgagaat tttggcaaat tcccaagcaa
     4201 gcttttcacg gctttggtaa tgggcatcgt acaacgtgtc tatcatggtc atcggcccag
     4261 gcagcgccca tttaatcggc tgcgaggtca attggcgtaa aaacttggca tcctcaacaa
     4321 acacaggccg agaacgcgcc acctcaccca ccaccgtggg cacattggca tcatagcggt
     4381 tgcgtatccg cacggtttca cgtttctcaa aatccacgcc actcaaatgt tcaataaacg
     4441 tggtgacgaa gtgttggcgg gtttgttcgc catcgctgac gatgtcaacg cctgccaaca
     4501 actgctcttg caaagacaca cgcaaagcat cttgtttgcc ttccagcaaa acctcttctg
     4561 acagcttcca aggtgaccaa agtttttcag gctccgccag ccaagagggt ttaggcaaac
     4621 tgcctgcggt agaagtgggt aatagtttat tcataatcat gcatctgttc tgtgtcattt
     4681 taagagatta aatcgcgtaa tttgcggccc attgttccag tgtattttgg tgtggcttga
     4741 tgaaatgctc ttcagtgaac ttgccttgct taatggccag ttgaccacgc tcttcacggt
     4801 catacacaat ttgagtcaag gaatggtctt gattgttcaa actaggctga taccatttac
     4861 cggccgctgc attggcgttg taaatttcag ggcggtaaat tttttgaaag gtttccatcg
     4921 tgctgatggt gccgatgagc tcaagattgg tgtagtccgc caacaagtcg ccgtgaaagt
     4981 aaaaagccaa aggggcaacg ctgttggcag gcataaaata gcgcacttgc aagcccattt
     5041 tgccgaaata ctgatcggtc aaagacgact catcttgcac atattccacg cccaaaacag
     5101 gatggcggtt ttcagtacga cgatagactt tgctgctgga aacactcaag caaatcactg
     5161 gtggcttgtt aaaatgctgt ttgtaaacat cggaacccac aaaatactga aacaacttgc
     5221 catgtaaatc cccaaaattg gcgggaatgc tgaatttctc ttggtttttg ttgtgctcca
     5281 acagcagcac actgaagtca taatcccgca cataagaaga gaaattattg ccaacaatgc
     5341 cttcaatgcg cttattggtt tggtgatcca atatgttggt tttcagtatt tcgatggtgg
     5401 gaaatgagtg ctcaccgctg gcagtgccaa ttttcatgtc caccgaaaca atctcaattc
     5461 caaccgaata gcgatcgcct ttgggattgt cccagctcgc caaatcattg aaacggttgt
     5521 ctatcatttt cagcgtgttg cgcaaatttt cttgacgact gctgcctctg gccaagttgg
     5581 cgaaattggt ggtgatgcgt gtgttgtctg acggatggta attctcatca aaacaaatgc
     5641 tcttaatctc aaaagtaaat tctttattca tggtaatccg ctaccttaat gtctgaggca
     5701 agactgcgat ggatggccca ttcaggtctt cccgattcaa ttcggtcttt gcctgtcatc
     5761 aactgtgtcg atgtttcatg ctgtgtcacc caacagctct atcaaaacat ctgcaaacac
     5821 agcccacttc aaggcgcttg gtaggattta tactggaatc aaaacatgaa taaaaatgat
     5881 attatttcat caaatcatga acatcattca tcaattcaat ataaagttga aatgaaatgc
     5941 ccattcttta aacagattct tgcgaacttt acccattaac tgaattgttc aagcagttaa
     6001 aagcaatctg gattgatggt caatgccaca tccaattgca ggtgacacat acccatcact
     6061 gcatgaaaaa tcaaatgcat acacataaaa acaagcggcc tgtaacattt acaggccgct
     6121 tgtttgctta cacgattagg ctgctttaga tttcagtcac tgccaagcgt ggataggttt
     6181 tttcacaata gtgacaacgc aatttggttt caccgttggc ggttttcact ttgaacgagc
     6241 tgatgacagg ctctttgtgc gaggcgcaat tgtcattcgg acagcggaac aagcctttca
     6301 catgctcggg caaattcaaa ctgagcttgt ccaccacttt gtagtcggct atcacgttca
     6361 ccgtggcatt gggcgcgaac aaggccaagc gattggcttg ttcggcatca aagcgcacgt
     6421 tttccacttt gatgatgtct ttgtggccca tgtttttgct gctcaaatta aagcccacgg
     6481 tcacgcgttc gcccaaatcg tttaaaccga attggcgcaa aatcaccaag cctaggcctg
     6541 cgggaatgtg gtcgatgacc gtgccgtcac aaatcgcttc tacgctgcgt tgttgggtat
     6601 tcatgctggc aactcctcat tcaaaaccgc agacaaaatc gcttgacggg caaacacgcc
     6661 gtttttcgcc tgttcaaaat aatacgcatg ttgggtggca tccacatcgg tagcgatttc
     6721 gtccacgcgt ggcaaggggt gcatgatgcg catattgtct ttgacattgc tgagcatggc
     6781 ggctttgagt acgtacaaac cttggatttt caagaattct tgttcgtcaa aacgctcgcg
     6841 ctgtacgcgc gtcatgtaca cgatgtcggc cacgttcaag gcttcttcaa agctgtctaa
     6901 gatttggtaa gcacagcctt tttcatccaa ctcttcggtg atgtagtcgg gcatcgccaa
     6961 ttgcggcggc gccacaaagt aaaacgtgtt gttccaattg ctcaaggctt gcgccaatga
     7021 atgcacggtg cggccatatt tcaaatcgcc caccaacaca atattgaggt gctccaagcg
     7081 gccttgggtt tcaaaaatcg tgattaaatc gagcaaggtt tgcgatgggt gttggttggt
     7141 accatcgcct gcattcacca cgggcacgct ggaaaattcc gccatcacgc gcgcggcgcc
     7201 gtctttgttg tggcgcacca ctatcgcatc ggcataacct gaaatgatgc gcgcggtgtc
     7261 ggccaaggtt tcgccctttt tggcgctggt gttggcgccg tcggcaaaac caatcacatt
     7321 cgcgcccaag cggtgggcag cggtttcaaa cgaaagacgg gtgcgggtag aaggctcgaa
     7381 aaaacaggtg gcgaccaatt ggtcggagag caaatcaggt tgaggatgtt gtttcaaatg
     7441 aagcgcggtt ttgaccaact gagtcaaggt atcggccgac aaatcggcca ccgagatgac
     7501 gtgttggcga tagagcggat tgggcatggt tttccttcaa ggcaactgaa tcgcaagacg
     7561 gtggcggcga tgaaaccgcc acgcaaacgg ctgacatgat acgctcaatc gcagccagaa
     7621 aaaagccacc atcgcttcaa accaaccgtt tacaggtcgc tttatttggc tgcgcgcaaa
     7681 cgcgctaatt ccaaacgcac ttggttcacg gtgtcttggt tgtacatttt tggaatgttg
     7741 cgccacacgg tatgaaaacc atatgggcct tccaccattt cgcgtttggc ttcttctaca
     7801 ctccagcctt ggttgacgat gcggtacatg ccgatgatta agccagtgcg gtcggcgccg
     7861 tggtagcaat gcaccaacac cccgccttgt ttttgcgctt tttcaatgtc gtacaacacg
     7921 gcagcgattt ctttgggctt gatgcgccat gacttcagcg gatgattcac caaattcagc
     7981 tttaaatgtg acaactcttc tttgtcgtca tcgcggtcaa aaaagcgcaa attgatgatg
     8041 gttttgatgt tgtgttgttg tagcaaagcc tcgtctttgg cggtgagttg ctcactgcgg
     8101 tacaaattgg gtgtgacacg gaacaaattc acgctttgct ccaccggtgt agcccacgtg
     8161 tattgcaccg caggcgcttc aatcgcggca atgcgcttgg cctcagcagg cgcgcacaca
     8221 cccaaggcca gcatcacaca caaataaccc aaattcattg catccacagc attcccctgt
     8281 taatcagatg aaaggcactg taacacaatc gggttttaac tttggttaag ccatcgcttt
     8341 gacacggggt tgatttccgt tacaatccgc acatgactac tcccatccgc attttaggca
     8401 tagaccctgg cagccgcgtc acgggctttg gcgtgattga tttgcttggc cgccagcaaa
     8461 cctatgtggc ttcaggctgc atcaaaaccc cgcccaaagc ggcattatcc gagcgcattg
     8521 cggtgttgtt tgagcacatt tccgaagtca tagacacata tcagcccacc gtcagcgcga
     8581 ttgaacaggt gtttgtgaac gtgaaccctg cggccacttt gatgctcggt caggcacgtg
     8641 gtgcgtgtgt ggcggctttg nnnnnnnnnn cggcaaattg cctgtgcatg aatacactgc
     8701 cttgcaagtg aaacaagcgg tggtgggcca aggcaaggct gccaaagaac aggtgcaaca
     8761 catggtggtg caaatgctgc gcctcaatgg tacgccatcc agtgatgctg ccgatgcttt
     8821 ggccgtggcg attacccatg cgctgcgcaa ccacaatttg gcggcgcaaa tccaacaagg
     8881 cttgagcgtg aagcgcggtc gcttcaccac ccgttaaacc ttgtaccccc tctttacagg
     8941 ccgcttattt cacatgcggc ctgtaaaccc aaaacactaa gggaaaccat gatcagtcaa
     9001 ctgacaggca gcatcatcga caaacaaccg cccctcatcg tgattgatgt gaatggcgtc
     9061 ggctatgaag tgttcgtgtc catgcaaacc ttttacggct tgcctgccac gggcgaaaaa
     9121 gtgcgcatct acacgcaaat ggtggtgcgc gaagacgcgc atttgctgtt tggctttggc
     9181 aacaccatag aacgcgaaac cttccgcttg ttgattaaag tgtcgggcat aggagcgaaa
     9241 acggctttgg gcattttgtc ggtgttgtcc agcgatgact tggcgcgcgc gattgccgat
     9301 gaagacatca aagttttgag ctcagt
//
LOCUS       DAOMVK010000025         9207 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_1901_length_9207_cov_3.282780, whole genome shotgun sequence.
ACCESSION   DAOMVK010000025 DAOMVK010000000
VERSION     DAOMVK010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 9207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9207
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1901_length_9207_cov_3.282780"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..1441)
                     /gene="waaA"
                     /locus_tag="PK856_01785"
     CDS             complement(176..1441)
                     /gene="waaA"
                     /locus_tag="PK856_01785"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003683901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HRL01133.1"
                     /translation="MYLGVYQLLWKIAPPLVRRYLRKRGRNAPAYLQHWGERFGEPHP
                     APIQGAIWIHAVSVGETRAAAPIIAALKKHFPAAPLLLTQMTPTGRETAEHLYPDAQC
                     RYLPYDRPDYVAQFLQEHRPMFGVLMETELWPNWILGADQAHVPLFLANARLSEKSLH
                     GYQKAASLFTPVMAKLSAVFAQTEADAERLRLLGAQQVSVCGNSKYDIAPPQAALDLA
                     ADFKTRIGMRPVIVCGSTRVDKQGVDEAELMLKAWQSADSNALLVVVPRHPERFDAVF
                     QLALNLGFKTQKRSDGESVAADTQVWIGDSMGELFAYYACCDVAFVGGSLVDTGCQNV
                     IEPIACGKPTLFGYSTYNFQAACDAAVAAGVAKQVHTADEWRVSTLALLQNAEQQVAM
                     QQQAQAFIHAHQGASLRIAEQIAQCYRKP"
     gene            complement(1506..5255)
                     /locus_tag="PK856_01790"
     CDS             complement(1506..5255)
                     /locus_tag="PK856_01790"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HRL01134.1"
                     /translation="MKRLVWTLMSVLIILMLTLLWLTKTESGLRFTLFRIPAWFGVEI
                     KADKVSGSVWNGFKGENWVVKTEGANINLRNLDIDWASSELFSRTIHVRKIDIGEMHI
                     DVMAAEPRPDQESTFPQSLRLPANVHLDEINIAKITMGKNHDVILNQAKLNYHYQHGQ
                     AHKVVIDKLDSTFGIADGHIALGESKPFATDAKLNVLGSRDGITVKGGVGISGDLDKT
                     LVQGKFTGEGVLLDIDGVLHPFAKTLNKQFDHLNISGVGLNPKTIIPTLPEADLKLNL
                     AIEPIAGSNELSGMLNVTNGKPLPADGNGIPVKSVVGKFKLDHDGKIVISPSSITTLK
                     QGIVTAQGTVDVPKQQLDLNVGLASLTLADVIQNPMVGTLNGDVAVTGQFDTPQADWQ
                     LKTERLQSTDRSNGQQSLKISRARIATPDGGAVGVAGTMNLYKEQEIKLALVSHRFNP
                     AALSKDYPQGNVNLDARVMGTLAGEPVIRSILDLGNSTLSGAKLVGNGDVIFQQQHLS
                     RANLNVLLDGNRIKTDGSIGKTGDKLNVDIHAPRLDQFGFGLSGLLTAKGHVAGDPKK
                     LDIQLAGNAQGLKFQDDVNVQQLRFDVSASPDMAAPMKVDIHGNTMKFGGTAVDIINL
                     AVKGTGRNHNIQADASLKMDNKPYKLLLAAGGGLDDKMQWKGAVSTLDISGAFNAKLR
                     NRMQLEAGSERVAMSSASWGLMGGGLNLQNFVWEAKTGLRTKGNATGLDIRQIDNLVQ
                     MPLEQNVIIGGDWDLSYGHAMSGFLRLNRQSGDIKLPQRNQMLGLSRLQTDTRFTPGN
                     ISTKVDIVTQYARANGQINIGQNYGNDINNAPLSGGVKVIIDDIAKVRNFLPVGMEVT
                     GRAQADVRLGGRLGAPQLSGPFTGSNLSFLDRSSGLYLKNGSLQSHFSGQSWVIEGLT
                     FREGAGSARVHGRVNLIGTDPDVDVKVTLDRFEALAKPTQQLTLSGEANLLYLPVKGL
                     TLTGLVKVDKARFDFPKSSAPSLSDDVVVLGRPEKEQATSTPINLDLTLDLNNAFRFS
                     GQGLDVLMGGQLRLLAKPGEDVQGQGQIKVVRGRYKAYGQDLIIRRGYITFIDELTNP
                     TLNIRAERRASPVGAGVEVTGSLEKPNTVLVADEAMSDKDKLSWLILGRPSSGDSDDA
                     AIAAAAGAWLAGNINDKVGIVDNIGLETRRTRNTETGEMNAAEQVINVSKRLTNRLSV
                     GYEYGLTSAESTVKLIYFISRSFQVIGRVGTNSSGGEVRYTRRFD"
     gene            complement(5409..7292)
                     /locus_tag="PK856_01795"
     CDS             complement(5409..7292)
                     /locus_tag="PK856_01795"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HRL01135.1"
                     /translation="MPMLLCPVSGWASAVASQNGLSGTKQANWETVSITARPLAVAEQ
                     EVVNERKKAAGKDGKEIELAKPSELQYPILVETTDKQATEMLNEHLPLIVQQQMNNSI
                     DAEQIQFLVEDTPKNAKNMLDTLGYFNATVSVTPKDKGYAIKVELGSRTKIESVTLNL
                     AGPILDDEKLGEYYANAMEGWTANVGEPFLQDTWSSSKSSAVSGVRRKKYPLAKMTHS
                     KATIDPAQNSAKMEAEVNSNQAIFFGELIVKGTQRYPESIVHKLSKFKAGDPYDSDQV
                     VDLQNALEQDSHYSGVFVSPKFDQLQGDRVPMEVTVQEVPRQKFDAGLSYDSEEGPGM
                     RLGYEHYNVLNRGYVGSSFMSYNKYEQSFGVGLSQPREVNGSYNTASINYKNSTVQKV
                     KTKAWSAGVWHVKERDRSEIRLGLEHYRDDSFVENGPDYGVNYATMLTASWRYNTIET
                     KMRPQHGYYFYGKVGSTVGNLLSSSSMQMAKADAAYYFTPEKKKYGTFIVRGQGGLVN
                     VSDERNAPQSLLFRTGGANSVRGYEYESIGIRGFEDAVMGGKVMATASLEYQLPIKQD
                     YALAVFHDVGGVSNSIGDFTLKHGTGIGGRWFSPFAPIAFDLAYGHDDQKIRWYIGLG
                     TQF"
     gene            7698..8123
                     /locus_tag="PK856_01800"
     CDS             7698..8123
                     /locus_tag="PK856_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01136.1"
                     /translation="MQNTTTQPTVAATTSNMAVDQALVALTDDVTIIPKSTQDVVMKV
                     DLRHTEPVAVKTKVKNQPSATKVAYRAAPVKAVAVKPQLTRSQIVRNELNREKAALRT
                     AENNLASARKTGKNASQLERQVADRRASISAMQSELSRL"
     gene            complement(8125..9030)
                     /gene="rbsK"
                     /locus_tag="PK856_01805"
     CDS             complement(8125..9030)
                     /gene="rbsK"
                     /locus_tag="PK856_01805"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HRL01137.1"
                     /translation="MSSNILVVGSINMDLITVLERFPKVGETLLGQTFTTALGGKGSN
                     QAVAAARLGAQVAMIGAVGDDANGDAALKILQQEGVDCSGIAVKTGMSTGIASITVAE
                     GDNHIIVVSGANFALSLADIDAHADLFAAADVVLCQLEIPMDCVLHAAQLAKQHGATF
                     VLNPAPAQALSAELLALVDVMTPNHTELKQAFALSETASDADIVQMLPTHMQLVMTEG
                     ANGASHYSAAGKRHWPAFKVDVVDTTGAGDTFNAGLAVYWTQGLDVAVQHAQASAALA
                     VQKLGAQTAMPTASAVGKFIKQQTS"
BASE COUNT         2306 a         2532 c         2012 g         2357 t
ORIGIN      
        1 agcacatcga gtcgggcgga ttttacgtca aatagtgaat tcattctaat ctacaagaaa
       61 agctttattt tgagtggtta gagtttaccg tgatagttgt gtcgacacaa tcaagcacgg
      121 cctaatttac tgcactgtca tgcgcaaggc ttttgttacg gcctgtgcta tcaaactaag
      181 gcttacgata acactgggct atttgttctg caatgcgcaa actcgctcct tggtgcgcat
      241 ggataaacgc ttgcgcttgt tgctgcatcg ctacttgttg ttcagcattt tgcagcaaag
      301 ccaatgtact gacccgccat tcgtctgcgg tgtgcacttg cttggccaca cctgcagcca
      361 ctgcggcatc acaagcggcc tgaaaattgt aagtagaata gccaaacaaa gtgggcttgc
      421 cacaggcaat gggctctatc acattttgac agcccgtatc gaccaaactg ccgccaacaa
      481 acgccacatc gcaacaggca taataagcaa acaattcacc catgctgtcg ccaatccata
      541 cttgcgtgtc ggccgccaca ctctcgccat cgctgcgctt ttgtgttttg aaacccaaat
      601 tcaatgcgag ctgaaacacc gcatcaaacc gctcaggatg gcgcggcacc accaccagca
      661 aggcattgct gtctgcgctt tgccaagcct taagcatcaa ttcagcctcg tccacacctt
      721 gtttgtccac acgggtgctg ccgcaaacga ttacaggccg catgccaatg cgtgtcttga
      781 agtcagcagc caaatccaag gcagcttggg gtggtgcaat atcgtatttg ctgttgccac
      841 acacactcac ttgctgcgct cccagtaagc gcaaacgctc tgcatcggct tcggtttgtg
      901 caaacaccgc actcaacttc gccatcacgg gcgtgaacaa tgaagcggct ttttggtagc
      961 catgcaaaga tttctcagac aaacgggcat tggccaaaaa caaaggcaca tgtgcttggt
     1021 ctgcacccaa aatccaattc ggccacaatt cggtttccat caacacgccg aacatggggc
     1081 gatgctcttg taaaaactgc gccacataat cgggcctgtc ataaggcaaa taacggcact
     1141 gcgcatcggg atacagatgt tcagcggttt ctcgcccagt tggcgtcatt tgcgtgagca
     1201 acaatggcgc agcggggaag tgttttttta aagccgcaat gatgggtgcg gcggcacggg
     1261 tttcccccac agaaacagca tgtatccaaa tagcgccttg tatgggcgcg ggatgaggct
     1321 caccaaaccg ctcaccccag tgctgcaaat acgcaggggc attgcggcca cgtttgcgca
     1381 aataacggcg caccaaaggc ggtgctattt tccacaacaa ttgataaaca cccaaataca
     1441 tggtgtgtga catcctttac acaagcggcc tgtaatgatt tacaggccgc ttgtttcaac
     1501 ttcaattaat caaaacgacg ggtatagcgc acttctccac cgctggaatt tgtgcccacg
     1561 cgacctatca cttggaaaga acggctgatg aaataaatca atttcactgt cgattccgca
     1621 ctggttaagc catattcata acccacactc aaacggttgg ttaaacgctt gctgacatta
     1681 atcacctgct cagcagcatt catttcaccc gtttcggtat tgcgggtacg gcgggtttcc
     1741 aaaccaatgt tgtcaacaat gcccacttta tcgttgatgt tgcccgccaa ccacgcacct
     1801 gccgcagctg caattgccgc atcatcactg tcgccagatg aaggtcgacc caaaatcagc
     1861 cacgacaact tgtctttatc actcatcgcc tcatcggcca ccaacacagt gttgggtttt
     1921 tccaaactgc ccgtgacctc cacgcctgcg cccactggag aagcgcggcg ttcagcacga
     1981 atgttcaagg ttggattggt caactcgtct atgaatgtga tgtagccacg gcggataatc
     2041 aaatcctgac cataggcttt gtagcgaccg cgcaccactt tgatttggcc ttggccttgc
     2101 acatcctcac ctggtttggc caacaagcgc aattgtccgc ccatcaacac atccaaacct
     2161 tgaccgctga agcgaaatgc attgttcaaa tccaacgtta aatccaaatt aatcggcgtg
     2221 ctggtagcct gttctttttc aggccgcccc aacaccacca catcatcgct gagcgaaggc
     2281 gcactggatt taggaaaatc aaaccgagct ttgtccactt tcaccaaacc cgtcaaagtc
     2341 aatcctttga ctggcaaata cagcaaattg gcttcaccgc ttaaggtcag ttgttgagtc
     2401 ggtttggcca aggcttcaaa tcggtctaga gtcactttca cgtccacatc agggtcggtg
     2461 cctatcaaat tcacccgccc atgcacacgc gccgaacccg ctccctcacg gaaagttaag
     2521 ccctctatca cccacgattg accagaaaaa tgcgattgca agctgccatt tttcagatac
     2581 aggccgcttg aacggtctag gaaactgagg ttactgcctg tgaacgggcc tgataattgc
     2641 ggcgcgccca agcgcccacc caaacgcaca tcggcttgag cacgacccgt cacttccatg
     2701 cccactggca agaagttgcg cactttggcg atgtcatcaa tgatgacttt cacaccgcca
     2761 ctcaaaggcg cattgttgat gtcattgcca tagttttgac caatgttgat ttgtccattg
     2821 gcacgggcat attgggtgac aatgtctact ttggtgctga tgttgcctgg tgtgaaacgg
     2881 gtgtcggttt gcaatcggct caaacccaac atttgattgc gttgcggcaa tttgatgtca
     2941 cccgactgac ggttcaaacg caggaagcca gacatcgcat gtccatacga caaatcccaa
     3001 tcgccaccga taatcacgtt ttgctcaagt ggcatttgca ccaaattgtc gatttggcgg
     3061 atgtccagcc ctgtggcatt gcctttggtg cgcaaacctg ttttggcttc ccaaacaaag
     3121 ttttgcaaat tcaagccgcc acccatcaat ccccatgaag cactgctcat ggccacgcgc
     3181 tcactgcctg cttccaattg catgcgattg cgcagtttgg cattgaatgc gccgctgatg
     3241 tccaaagtag aaacagcgcc tttccattgc atcttatcgt ccaaaccacc acccgcagcc
     3301 aacaacaatt tatacggttt gttgtccatt ttcaggctgg catcggcctg tatgttgtga
     3361 ttgcggcctg tacccttcac agccaaattg atgatgtcta ctgccgtacc accaaacttc
     3421 atggtattgc catgtatgtc cactttcatc ggtgccgcca tgtctggaga cgcactcaca
     3481 tcaaaacgca attgctgcac attgacatcg tcttgaaatt tcaagccttg ggcattgcct
     3541 gccaactgga tgtcgagttt tttcgggtcg cccgccacat ggcctttggc cgtgagcaag
     3601 ccactcaaac caaaaccaaa ttgatccaag cgcggagcat gaatgtccac attcagtttg
     3661 tcacccgttt tgcctatgct gccatcggtt ttgatgcggt taccgtccag caaaacattc
     3721 aaattggcac ggctcaaatg ctgttgctgg aaaatcacat cgccattgcc caccaatttg
     3781 gcacccgaca aggtactgtt gcccaaatcc aaaatgctgc gtatgacagg ttcacccgcc
     3841 aaagtcccca tcacccgagc atccaaattg acattgcctt gtggataatc tttagacaga
     3901 gcggcagggt tgaagcggtg gctgaccaaa gccagcttga tttcctgttc tttgtacaaa
     3961 ttcatcgtac ctgccacccc cacagcaccg ccatcaggtg tggcaatgcg ggcacggctg
     4021 attttcaaac tttgctggcc attgctacgg tctgtcgact gcaatcgctc ggttttcaat
     4081 tgccaatcgg cttgtggggt atcaaattgc cctgttaccg caacatctcc attcagagtg
     4141 ccgaccattg ggttttgtat cacatcggcc aaggtcaaac tggccaagcc cacattcaaa
     4201 tccaactgtt gcttcggtac gtctaccgta ccttgggcag tcacgatgcc ttgttttaag
     4261 gtggtgatgc ttgagggtga aatcacaatt ttgccatcgt gatccaattt gaattttccg
     4321 accactgact tcacaggaat gccattacca tctgcgggca agggcttgcc attggtgaca
     4381 ttcaacatgc ccgataactc attgctgcct gcaatcggct ctatcgccaa atttaatttc
     4441 aggtctgctt caggcaaagt gggaatgatg gttttcgggt tcaaacccac gccactgatg
     4501 ttcaaatgat cgaattgttt gttcaaggtt ttggcaaatg ggtgcaacac gccatcgatg
     4561 tccaacaaaa cgccttcacc cgtaaattta ccctgaacca aagtcttgtc caaatcgccc
     4621 gaaattccga caccaccttt aactgtgatg ccatcacggc tgcccaagac attcaatttg
     4681 gcatcggtag caaacggctt gctctcaccc aaggcgatgt gtccatcggc aataccgaag
     4741 gtgctgtcca acttatcaat aacgactttg tgcgcttgac cgtgttggta atggtaattg
     4801 agcttggctt gattcaagat cacatcgtgg tttttgccca tggtgatttt ggcaatgttg
     4861 atttcatcca aatggacatt ggcaggcaaa cgcaaacttt gcggaaacgt ggattcttga
     4921 tcagggcgtg gttctgctgc catcacatcg atgtgcattt cgcctatgtc gattttgcgc
     4981 acatgaatgg tgcgtgaaaa caactcactg gaggcccaat cgatgtccag attgcgtaaa
     5041 ttgatgttgg ctccctcggt tttcaccacc cagttttcgc ctttaaaacc attccacact
     5101 gagccagaaa ccttatccgc tttgatttcc acgccgaacc aagccggtat gcggaacaaa
     5161 gtaaagcgca aacccgattc ggttttggtc agccacaaca aggtcagcat caagatgatt
     5221 aacacagaca tcagcgtcca caccaaacgc ttcaaccacc gccattttga aactggtttt
     5281 tttggcggag gtgtttggct ttctggctgt tctaatgttt cacttgttac actgatttgg
     5341 ctcatacacc gtactcaata ctctgataaa tggacacaga cacatgcggc ctgtaaaaac
     5401 tgaacggctt aaaactgagt gcccaagccg atgtaccacc gaattttttg atcgtcatgg
     5461 ccataggcca agtcaaaggc aatgggagca aaagggctga accaacgtcc acctatgccg
     5521 gtgccatgct tcaaggtaaa atcacctatg ctgttggaaa ccccacccac atcgtgaaac
     5581 accgccaagg cgtaatcttg tttgatgggc aattggtatt ccaagctggc ggttgccatc
     5641 accttgcccc ccattaccgc atcctcaaag ccgcgtatcc ctatgctttc atattcatag
     5701 cctcgcaccg aattggcccc gccagtacga aatagcaagg attgtggtgc attccgctca
     5761 tcactgacat tgaccaaccc accctgacca cgcacgatga acgtgccgta ttttttcttt
     5821 tcaggcgtga agtaataagc ggcatcggct ttggccattt gcatagaaga actggacagc
     5881 aaattgccca cagtgctgcc tactttgccg taaaaataat agccatgctg cggccgcatt
     5941 ttggtttcaa tggtgttgta gcgccaagat gctgtcaaca tggtggcata attcacaccg
     6001 taatcagggc cattctccac aaaactgtca tcacgataat gctccagacc caagcggatt
     6061 tcactgcggt cacgttcttt cacgtgccac acgcctgcgc tccaagcctt ggttttcact
     6121 ttttgaaccg tgctgttttt gtaattgatg gacgcggtgt tatacgaacc attgacctca
     6181 cgtggctgac tcaagcccac accaaaactt tgttcgtatt tgttataaga cataaaactc
     6241 gagcccacat acccgcgatt gagcacattg taatgttcat agcccaagcg cataccgggg
     6301 ccttcttcag aatcgtagct taggcctgcg tcaaattttt gacgaggcac ttcttgtacc
     6361 gtcacttcca tcggcactct gtcaccttgc aattggtcaa atttggggct gacaaacaca
     6421 ccgctgtaat gactgtcttg ctctaaggca ttttgcaaat ccacaacttg gtcactgtca
     6481 tacggatctc ccgctttgaa tttagacagt ttgtgcacaa tgctttcagg gtagcgctgt
     6541 gtacccttta cgattaattc gccaaagaaa atagcttgat tactgttaac ttcggcctcc
     6601 attttggccg aattttgcgc aggatcgata gtggctttgg agtgggtcat ttttgccaat
     6661 gggtatttct tacggcgcac gccactgacc gcggagctct tgctgctgct ccaagtgtct
     6721 tgtaagaagg gttcgcccac attagcggtc caaccttcca ttgcgttggc gtaatactca
     6781 cccaattttt catcgtctaa gatgggtcca gccaagttca aggtcacact ttcaattttg
     6841 gtgcgcgatc ccaattccac tttgatggca taccctttgt ctttgggcgt gacactgaca
     6901 gtggcattga aataacccaa agtatccaac atgtttttgg cgtttttagg ggtgtcttct
     6961 accaaaaact gaatttgttc cgcatcaatg ctgttgttca tttgctgttg cactatcaat
     7021 ggcaaatgtt cattgagcat ttcggtggct tgtttgtctg tggtttcaac caaaatgggg
     7081 tattgcaact cactgggttt ggccaattca atttctttac catctttacc ggccgctttc
     7141 ttgcgctcat tcaccacttc ttgttcggcc acagccaagg gacgagcagt aatggacaca
     7201 gtttcccaat tggcttgttt ggtgccgctt aatccatttt gtgaggccac tgccgaagcc
     7261 cagccactca cggggcataa caacattggc attaacagat acgtccaaca ctgcctaggt
     7321 ttaaacacca tattctgaac acctaacctc atctactggt ccacacaagc cacaaatgag
     7381 gccaattcta acacaggtat ttactcagtt tcagacacca cctgatggag cagaatgatt
     7441 tagcgggttt taacgcagct ttgcatgaaa cccatgccaa ccaacaaaaa aagtcttata
     7501 attcattggt tttcaattac aagccgtctc aaacaaaaaa tatgcacata cttcgtcaca
     7561 gccatacggt attgacttgt ttattcatta ctttttcact gactggagcc atagcctctc
     7621 agccagcgca attaccttgc tcagccttac aacaaaacaa taaccaatct gtctgtatgc
     7681 gcagccacaa tctgagcatg caaaacacca caacccaacc cactgttgct gccaccactt
     7741 caaatatggc agtagaccaa gccttggtag ccttaaccga tgacgtgacc attattccca
     7801 aatcaaccca agatgtggtg atgaaagtgg acttgcgcca taccgaaccc gtggcagtga
     7861 aaaccaaagt taagaatcaa ccgtctgcga ccaaagtggc ttaccgagct gcgcctgtga
     7921 aagccgtggc ggtgaagcca caattgacgc gctcacaaat cgtgcgcaac gaattaaacc
     7981 gtgaaaaagc cgcactgcga acagctgaaa acaacttggc cagcgctcgc aagacaggta
     8041 aaaatgccag ccaattggaa cgccaagtgg ccgaccgccg cgcgagcatt tctgccatgc
     8101 agtctgaatt atcccgcctg taatttaact tgtttgctgt ttgataaatt tgcccactgc
     8161 tgatgcagtg ggcattgctg tttgtgcgcc caatttttgc acagccaaag ccgctgatgc
     8221 ttgcgcatgc tgcaccgcca catccaagcc ttgcgtccaa tacaccgcca agccagcatt
     8281 aaaggtatcg cctgcgcccg tggtatcgac cacgtccact ttaaacgcag gccaatggcg
     8341 cttgcccgcg gcgctgtaat ggctggcacc attcgcgcct tccgtcatca ccaattgcat
     8401 gtgagtgggc aacatttgca caatgtcggc atcgctggca gtttcgctca aagcaaaagc
     8461 ctgttttaac tcggtatgat tcggtgtcat cacgtctacc aaagccaaca attcggctga
     8521 caaggcttgt gcaggtgcag ggtttaacac aaacgtcgca ccatgttgct tggccaattg
     8581 tgccgcgtgc agcacacaat ccattggaat ttccaattgg cacaacacca catcggcagc
     8641 tgcaaacaaa tcggcatgcg catcaatgtc ggccaagctc aaggcaaaat tggcaccaga
     8701 caccacgatg atgtggttgt cgccttcggc cacagtgatg ctggcaatgc ctgtgctcat
     8761 gcctgttttc acggcgatgc cactgcaatc cacgccttct tgctgcaaaa ttttcaaagc
     8821 ggcatcacca ttggcatcgt cacccaccgc acctatcatc gccacttgtg cacccaagcg
     8881 cgccgctgcc accgcttggt ttgagccttt gccgcctaaa gccgtggtaa aagtttgccc
     8941 caataaggtt tcgccgactt taggaaaacg ctccaacacc gtgattaaat ccatattgat
     9001 actgccgaca accaaaatat tgctgctcat aatgtgcttt cactttttaa ggtgttcaga
     9061 ataacaaatt ttctgcgaca ggaaaaagcg gcctgtaacg cttacaatgt aaacccatat
     9121 tgtttctgca ggtttcgccc ccatgtgagg gccaattccc ttttactttc cgtttcagaa
     9181 aaggcacgct cgtggacccg atacaaa
//
LOCUS       DAOMVK010000026         9095 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_1945_length_9095_cov_2.869027, whole genome shotgun sequence.
ACCESSION   DAOMVK010000026 DAOMVK010000000
VERSION     DAOMVK010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 9095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9095
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1945_length_9095_cov_2.869027"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..931)
                     /locus_tag="PK856_01810"
     CDS             complement(20..931)
                     /locus_tag="PK856_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRL01138.1"
                     /translation="MNPALQLVQKQLNGLILGKNAILDLVLACVLARGHLLLEDVPGV
                     GKTTLATGLAAVLGLTYQRVQFTNDMLPSDLLGVNVFHPQEARFTFHQGPVFNQFLLA
                     DEINRASPKVQSALLEAMEEGQVSVDGTTYVLPKPFMVIATQNPTEQLGTFPLPESQL
                     DRFMMRLSLGYPVAQAEKYLYAHGDRRQLLPKLQAALNSADLLALQQQVQDVQTNEAL
                     ADYVYRLVQETRKPGMFVLGLSPRSGLAIIAAAKAWAFLQQRQFVIPEDVQAVFPAVA
                     NHRLHSLQSNVSVDQLLQKIIKHVVVA"
     gene            complement(1007..1666)
                     /locus_tag="PK856_01815"
     CDS             complement(1007..1666)
                     /locus_tag="PK856_01815"
                     /inference="COORDINATES: protein motif:HMM:NF019960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HRL01139.1"
                     /translation="MRRSKNDAEKTKQLLVQAACEVFYREGVVRATLEQIAREAGLTR
                     GALYWHFNNKVDILDELFQWIMPNVDVLQTNMLVWPTECYWDNLIAHFMEFFQELQED
                     SSFRKFFTVMHLKCEMTESNAQVIELLRKYELIWERQVEGVISMAVSKKVLSEQTDVV
                     LTSKLLRSHLLGIIVVFLGDVEEKGLTETNYQVAIVQLQHMLRVLKDHTILKQGTIST
                     L"
     gene            1923..3191
                     /locus_tag="PK856_01820"
     CDS             1923..3191
                     /locus_tag="PK856_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003824296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRL01140.1"
                     /translation="MSSAHHPENRFKQPTFKATLLALVIATSLAACGGGDGKQAAAGQ
                     TPPPPVVSVVTVENTTVEVSNELPGRLEASREAQVRARAAGIVQKRLFTEGSYVKAGQ
                     PLFQIDNAPYVANLETARAQLATAEANLAKANADVARYAPLVSEGAVSKQEYDAAIAQ
                     QRLGRAQVQAAKAAIKTAQINVGYAYVTAPISGHIGRAMVTEGALVGQGEATQLATIQ
                     QTSTLYVNLTQATGEIMKLRQAMADGNITLVDGRPEVTILLEDGSEYGQKGRLLFTDV
                     TVDENTGQMALRAEVPNPDNILLPGMYVRVKVPQAEIANASLIPQQAVTRSNQGDIVM
                     VVNADGSFAPRPVKISRSQGSYWVVTSGLKQGDKVIVDGMAAVGMTQAKKVQTQAWKD
                     PSKNKAAPAAQAPATASAPAAKAASQPEAK"
     gene            3203..6529
                     /locus_tag="PK856_01825"
     CDS             3203..6529
                     /locus_tag="PK856_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRL01141.1"
                     /translation="MSKFFIDRPIFAWVVAIFIILAGVISIRGLPVEQYPQVTPPTIQ
                     LNVAYAGADAQTLQDSVLSIIERQMNGLEGMDYMESSAMSNGTGSLTITFKPGVNEDI
                     AQVNVQNRLSQAESRLPEAVKQVGVTVNKTTSNFLMFVMLYDESGKTPVGDIIDYISR
                     NVQPELQRIEGIGNVQVFGSEKAMRVWIDPAKLQGLNLSANDVNTAIRAQNAQVAPGT
                     LGDLPSTISATITVPGQLKTVEEFENIVLRANSDGSVVRLKDVAKVELGQQAYAAEAR
                     LNGKPAAGIGIQLTSTGNAVQASDDIRAKMEELKPFFPEGVQWKAPYDSSTFVGISIE
                     KVIHTLIEAMVLVFIVMFVFLQNIRYTLIPTIVVPIALLGAFAIMNLAGMSINVLSMF
                     AMVLVIGIVVDDAIVVVENVERIMAEEHLSPLEATKKAMSQIQGAVIGITVVLVSVFI
                     PMAFFTGTTGNIYRQFSLVMAVSILFSAFLALSLTPALCGTLLKPVDKDHHVKKGFFG
                     KFFNGFNRVFDRNAKRYESFVGKLLRRAGRIMVIYLVLIGVAAFVMTRIPTGFLPNED
                     QGFALSIIQLPPGATMERTLRTVETYEGFVTKQPEVENVVTVAGFSFAGQGQNMGMSF
                     VTLKDWAERGGKGQDAFALSGRITGAMMQQVKDGFVFATTLPAIPALGNGNGFSFRLQ
                     DRGEHGHLALVDARNKLMAAARQSPILVPQSVRAEGLEDAPQLRLDIDRDAANAQGVS
                     FASINSALSTAVGSSYVNDFPNQGRMQRVVVQSKPDSRMQSEDILAISVLNNQGKSIP
                     LSTFATATWVVGPMEVVQYNGYPAMKITGEAAPGYSTGDAMAEMERLSADLSSGFGYE
                     WTGVSKEEKAAGNQAAILYVFSILAIFLCLAALYESWSVPLAVILVVPLGLLGTVLGV
                     WGRGITNGLFPGSIYSFNNDVYFQVALITVIGLSAKNAILIIEFAKDLQAQGKSAAQA
                     ALAAAHLRFRPILMTSFAFILGVVPLYIASGASSASQRAIGTGVFWGMVIGTLLAVLF
                     VPVFYLIVRKLFKQTARQNERDAAHATAAGVTQEMSDHYVQKVEASLSDEDREALKEE
                     IKTELWAEYRAKKEKEDKHD"
     gene            6522..7991
                     /locus_tag="PK856_01830"
     CDS             6522..7991
                     /locus_tag="PK856_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRL01142.1"
                     /translation="MTDGKSTMVAKLSGLTVTVAAAMVLSACNLAPKYQQPQVFTDTS
                     EFKYATQTNSDIKAYDLGWQEYFADPQLKQLIGLALENNRDLRVAALNVEAVEKQYRI
                     QRSDLFPGINASGNMNRSRTPADFRGPNATAISKQYNVGLGVTAYELDLWGKIRNLSD
                     AALNEYFATSEARDSAQISLIATVAKAYYSERFANEAMKVSQNVLTTREKSYRLADLK
                     YKHGVSSKIDLRTAETLVESAKADYQSQLRAREQARNALVTLIGGQYPQDIATQASLD
                     KQFAYASLPSGLPSEMLLYRPDIRQAEYGLKAANANIGAARAAFFPSISLTGTIGSGS
                     VHLGDLFTGPNRAWSFMPQINIPIFNWGANKAALDLTRVRKEISVVQYEQAVQDAFQD
                     VSDVLVANATLKQQYASQLKSTNAEVDRNRLMQMRFNQGIADSLTMLDSQRQSYAAQL
                     ALLGVQQQWLNSRVDLYKVLGGGLKAYNDGKVQRLFTGE"
     gene            complement(8049..8900)
                     /locus_tag="PK856_01835"
     CDS             complement(8049..8900)
                     /locus_tag="PK856_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003769580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HRL01143.1"
                     /translation="MQPHAQEQAINAVIEHIRAAGLHEHVSVGAERVIIGAVGDERVL
                     NVQVLEQMPEVERAVRIVHDWRIISREAQPQNSVYVCRGVALGQEMLHIDIKRPVNAS
                     IVYLDPFYLPSNPYQVGVKSEEQQLRDLSGVVTDSHHNNQPVMARVRDLRQLDGVLLA
                     GADIVYLGGEWLDSRSMQQEIGRLNLPVVLCKDKHHTLEQWLVAAERVAMYGNHQIIL
                     GEAGTLSINHRYPYRLDIEAIAAAVKHSHLPVLANVIRLGNGLLNQNQLGQLAEIAGA
                     KIVLQQG"
BASE COUNT         2373 a         2266 c         2104 g         2352 t
ORIGIN      
        1 gcctttttgg cgcttgattc taggccacga ccacatgctt gatgattttc tgtaagagtt
       61 ggtctacgga aacattgctt tgcaaagagt gcaggcgatg attggccact gcgggaaaca
      121 cggcctgtac gtcttctgga atcacaaatt gacgctgctg caagaaagcc caagctttgg
      181 cagcggcaat gatggccaaa cccgagcgtg ggcttaagcc caaaacaaac atgccaggct
      241 tacgggtctc ttgtaccaat cgataaacat agtctgccaa tgcttcgttg gtttgcacgt
      301 cttgaacctg ttgctgcaat gccaataaat cggcactgtt caatgcggcc tgtaacttag
      361 gcaacagctg tcgtctgtct ccatgcgcat acaaatattt ttccgcttgt gccacggggt
      421 agcctaaaga caaacgcatc atgaagcgat caagctggct ttctggcaaa ggaaatgtgc
      481 ccagttgttc ggtgggattt tgggtggcaa tgaccataaa aggcttgggt aagacataag
      541 tcgtgccatc cactgaaact tgcccctcct ccatcgcctc aagcaatgcc gattgaactt
      601 tgggagaagc acggttgatt tcatcagcaa gtaaaaactg attgaagaca ggaccttggt
      661 ggaaagtgaa acgggcttct tgcgggtgaa acacattcac ccccaataaa tctgatggca
      721 acatgtcgtt ggtgaactgt acccgttggt aagtcaaacc caaaaccgct gccaaacctg
      781 ttgccagtgt ggttttgcct acgccaggaa catcttctaa caacaaatgc cctctggcca
      841 aaacgcaagc caataccaaa tccagtattg catttttacc cagaatcaaa ccattgagtt
      901 gtttttgaac gagctgcagt gctggattca tgcattattc ctgtgtatta atgtatttaa
      961 actaatttat gacgtgaata taccatcaaa cacagctaaa tcgcacttac aacgttgaaa
     1021 ttgtcccttg ctttaagatg gtgtggtctt ttaaaacacg caacatgtgt tgcaactgca
     1081 caatcgctac ttggtaattg gtttcggtca atcccttttc ctccacatcc cctaaaaaca
     1141 ccacgatgat gccaagcaaa tggctgcgca atagtttgga agtcagcacc acatctgttt
     1201 gctcagacaa cacctttttg ctcacagcca tagaaatcac accttcaacc tgccgctccc
     1261 aaatcaattc atactttcta agcaattcaa tcacttgggc attgctttca gtcatctcac
     1321 acttcaagtg catcacagtg aaaaatttac gaaacgaact gtcttcttgc aactcttgaa
     1381 aaaactccat gaagtgggcg attaaattgt cccaatagca ttcagttggc caaaccaaca
     1441 tattggtttg caacacatcc acattgggca tgatccattg gaacaactca tccaaaatgt
     1501 cgaccttgtt gttgaaatgc caatacaggg cgccacgcgt caatcctgcc tctcgcgcaa
     1561 tctgctccag cgtggctctg accacgcctt cacggtaaaa cacttcacaa gcggcctgta
     1621 ccaatagctg cttggttttc tcagcatcgt ttttgcttcg tctcatttgt ttttcttcga
     1681 cctcttagca ctgttaaaca aggaactttt accacataaa tccgcaccaa acttcgggct
     1741 tatcatgcaa tctttacctg cgtttaacat gaaatttcat tgactgaaca tacatcagtg
     1801 tatgtataat agcagattga cattcatcat tgcatccaca aacggtgctt tgtactaaaa
     1861 ttaacaacaa cctgttttac gcacaggatg cttacaccat taatcactag ggataatcaa
     1921 acatgtcatc cgcacatcac cctgagaacc gttttaaaca acccacattc aaagccacct
     1981 tattggcact cgtcattgcc acttcattgg ccgcttgcgg tggtggcgat ggcaagcagg
     2041 ctgctgctgg tcaaacacct ccgccacctg tcgtatcggt ggtaacggtt gagaacacca
     2101 cagttgaagt cagcaatgag ttgccaggcc gtcttgaagc cagccgcgaa gcccaagtgc
     2161 gcgcccgcgc agcaggcatc gtccaaaaac gcttgtttac cgaaggcagc tacgtaaaag
     2221 caggccaacc tttgttccaa attgacaatg caccttatgt ggccaacttg gaaaccgcaa
     2281 gagcccaatt ggcaacagct gaagccaatt tggccaaagc caatgcagat gtggctcgct
     2341 atgcaccatt ggtcagcgaa ggtgcggtaa gcaaacaaga atacgatgca gcgattgccc
     2401 aacaacgttt gggtcgcgct caagtacagg ccgctaaagc agccattaaa accgcacaaa
     2461 tcaatgtggg ttatgcctat gtaacggccc caatatctgg ccacattggc cgtgccatgg
     2521 tgacggaagg tgcgttggtt ggacaaggtg aagccactca attggccacc attcaacaaa
     2581 cctcaacctt atatgtcaac ctgacacagg ccacgggcga aatcatgaaa ctgcgccaag
     2641 ccatggcaga tggcaacatc accttggtag acggtcgccc tgaagtgacc attttgttag
     2701 aagatggctc ggaatacggt caaaaaggcc gtttgttgtt taccgatgtc actgtggatg
     2761 aaaacacggg tcaaatggcc ttgcgtgcag aagtccctaa ccctgacaac atcttgctcc
     2821 caggcatgta tgtgcgtgtg aaagtgccgc aagcagaaat tgcaaacgcg tctttgattc
     2881 cacaacaagc cgttacccgc agcaaccaag gtgacatcgt aatggtagtc aatgccgatg
     2941 gcagttttgc accgcgtcct gtcaaaattt ctcgctcaca aggcagttac tgggtagtga
     3001 caagcggcct gaaacaaggt gacaaagtca tagtggatgg catggcagca gtgggcatga
     3061 cccaagcaaa aaaagtccaa acccaagctt ggaaagaccc gagtaagaac aaagcggcac
     3121 cggccgccca agcccctgct acagcttcag ccccagcggc caaagctgcg agtcaacctg
     3181 aagccaaata aaggacggtt ccatgtctaa gttttttatt gaccgcccca tttttgcttg
     3241 ggtggttgcc attttcatca ttttggcagg ggtgatttcc atccgtggct tgcctgtgga
     3301 gcaataccca caggtaaccc ctcctaccat acaattgaat gtggcttacg cgggtgctga
     3361 tgcccaaaca ctacaagact cggttttgtc catcattgaa agacaaatga atggtcttga
     3421 aggcatggac tatatggagt cttcagccat gtccaatggc acaggctctc tcaccatcac
     3481 ctttaaacca ggtgtgaatg aggacattgc ccaagtcaac gtgcagaaca ggctgtcgca
     3541 agcggaatcc cgtttgcccg aagccgtgaa acaagtcggg gtaacggtca acaaaaccac
     3601 ctccaacttc ttgatgtttg tgatgctgta cgacgaatcg ggtaaaaccc ctgttggcga
     3661 catcattgac tacatttcac gcaacgtcca accagaattg caacgtattg aaggcatcgg
     3721 taatgtacag gtattcggct ctgaaaaagc catgcgcgtg tggatagacc cagccaagtt
     3781 gcaaggcttg aatttgtctg ccaacgatgt caacaccgcc attcgtgcac aaaatgccca
     3841 agtggctcct ggtactttgg gtgatttgcc ctcaaccatc agcgccacca tcacggttcc
     3901 tggtcaactc aaaaccgtgg aagagtttga aaacatcgtt ttacgtgcca acagcgatgg
     3961 ttcagtggtg cgcttaaaag atgtggccaa agtggaattg ggccaacagg cttatgctgc
     4021 tgaagcccgt ttgaatggca aacctgcggc tggtattggt atccaattga cctctacagg
     4081 taatgccgtt caagcatctg acgacatacg cgccaaaatg gaagagttaa aacctttctt
     4141 ccctgaaggt gtgcaatgga aagcaccgta tgacagctct actttcgtcg gcatttcaat
     4201 tgaaaaagta atccatacct tgattgaagc gatggttttg gtgtttatcg tgatgtttgt
     4261 gttcttgcaa aacattcgct acaccttgat tccaaccatt gttgtgccca ttgccttgct
     4321 tggcgcattc gccatcatga atttggcagg catgtccatc aacgtcttga gcatgtttgc
     4381 gatggttttg gtgatcggta ttgtcgtcga tgatgccatc gtggtggtgg aaaacgtgga
     4441 acggatcatg gcggaagagc atttatcgcc gctggaagcc accaaaaaag ccatgagcca
     4501 aattcaaggt gcggtaattg gtattacggt ggtcttggtc tctgtattca ttcccatggc
     4561 cttctttaca ggcaccacgg gtaatattta ccgccaattc tcattggtaa tggctgtatc
     4621 cattttgttc tcggctttct tggccctgtc tctgacacct gccttgtgcg gcaccttgtt
     4681 gaaacccgtt gacaaagacc accatgtgaa aaaaggcttc tttggcaagt ttttcaatgg
     4741 ctttaaccgt gtgtttgatc gcaatgccaa acgttatgaa agttttgtcg gcaagttgct
     4801 gcgccgtgca ggccgcatca tggtgattta cttggtgttg ataggtgtgg cagcctttgt
     4861 catgacccgt attccgacag gcttcttacc caatgaagac caaggctttg ctttgagtat
     4921 catccaattg cctcctggtg caaccatgga gcgtactttg cgcactgttg aaacctatga
     4981 aggctttgtg accaagcaac cagaagttga aaacgtggta acagtagcag gcttcagctt
     5041 tgctggccaa ggccaaaaca tgggcatgag ttttgtgacc ttaaaagact gggcagagcg
     5101 tggtggtaaa ggccaagatg catttgcatt gtcaggccgc atcacgggtg ccatgatgca
     5161 gcaagtgaaa gatggttttg tgtttgccac cactttgcct gccatcccag ccttgggcaa
     5221 tggcaatggc tttagcttcc gcttgcaaga ccgtggtgaa catggtcact tggctttggt
     5281 tgacgctcgc aataaactga tggccgctgc gcgccaaagc cccatcttgg taccacaatc
     5341 ggtgcgtgcc gaaggcttgg aagacgcgcc gcaattgcgc ttagacatag accgtgatgc
     5401 agccaatgcc caaggtgtga gctttgcttc catcaactcg gcattgtcta cggctgtcgg
     5461 ctccagctat gtgaatgact tccctaacca aggtcggatg caacgtgtgg tggttcaatc
     5521 caaacccgac tcacggatgc aaagtgaaga catcttggcc atttcagtac tcaataacca
     5581 agggaaatcc atccccttgt ccacgtttgc taccgctaca tgggtagtag gtccgatgga
     5641 agtggtgcaa tacaacggtt accctgccat gaaaatcacg ggtgaagccg ctcctggcta
     5701 cagtacgggt gatgccatgg ctgaaatgga gcgcttgtca gcggatttga gcagcggttt
     5761 tggttatgaa tggacgggcg tttctaagga agaaaaagcc gcgggcaacc aagctgccat
     5821 cttgtatgtg ttctcgattt tggccatttt cctgtgtttg gcagccttgt acgaaagctg
     5881 gagtgtgcct ttggcagtga ttttggtcgt gcctttgggc ctgttaggta ctgtgttagg
     5941 tgtgtggggt cgtggcatta ccaatggtct cttccctggc agcatctaca gtttcaataa
     6001 cgacgtgtat ttccaagtgg cattaattac agtgattggt ttgtcggcaa agaatgcgat
     6061 tttgattatt gagtttgcca aagacttgca agcccaaggc aaatccgcag ctcaagcggc
     6121 tttggctgct gcacacttgc gtttccgccc tattctgatg acttcatttg ccttcatctt
     6181 gggtgttgtg cctttgtaca ttgcttctgg tgccagctct gccagccaac gtgccattgg
     6241 tacaggcgtg ttctggggca tggtcattgg cacgctgcta gccgtgttgt ttgtgccagt
     6301 gttctacttg atagtgcgca aattgttcaa acaaaccgca cgtcaaaatg aacgcgatgc
     6361 tgcgcatgca actgctgctg gtgtgactca agagatgagc gatcactatg ttcaaaaagt
     6421 ggaagcctca ttaagcgacg aagaccgcga agccttgaaa gaagaaatca aaaccgaatt
     6481 gtgggccgaa taccgcgcca agaaagaaaa agaggacaag catgactgat ggtaaatcta
     6541 ccatggtcgc caaattgtct ggtttgacag tgactgttgc tgcggcaatg gtactgtcgg
     6601 cctgtaactt ggcaccgaaa taccaacaac ctcaagtgtt tactgacact tctgagttta
     6661 aatacgccac ccaaaccaac agtgacatca aagcctatga tttgggttgg caagagtatt
     6721 ttgctgatcc gcaattaaag caattgatag gtctggcatt agagaataac cgtgatttgc
     6781 gcgtggctgc cttgaatgtg gaagcagtag aaaagcaata ccgtattcaa cgctcagacc
     6841 tgttcccagg catcaatgcc agtggcaaca tgaacagatc acgtactcct gctgatttcc
     6901 gtggccctaa tgccaccgca atcagcaaac aatacaatgt gggcttgggc gtgacggctt
     6961 acgaattgga tttgtggggc aaaatccgca acctcagcga tgcggcctta aacgagtact
     7021 tcgctacaag tgaagcacgt gactcagctc aaatttcttt gattgccact gttgccaaag
     7081 cctattattc agagcgcttt gccaatgaag ccatgaaagt ttctcaaaat gttttgacca
     7141 cgcgtgaaaa aagctatcgc ttggccgatt tgaaatacaa acatggtgta tcgtccaaga
     7201 ttgatttgcg tacggccgaa accttagtgg aatcagccaa agcagactac caatcacaat
     7261 tgcgtgcccg tgagcaagcc cgtaatgcat tggtaaccct gataggtggt caatatccac
     7321 aagacattgc tacgcaagca agcctagaca aacagtttgc ttatgctagc ctgccttctg
     7381 gcttgccatc tgaaatgttg ctctatcgtc ccgacatacg ccaagctgaa tatggcttga
     7441 aagctgccaa tgccaacatc ggtgccgctc gtgccgcatt cttccccagc atcagcttaa
     7501 ctggcaccat aggctcaggc agtgtgcatt tgggtgattt gttcacagga cccaaccgtg
     7561 cttggtcttt catgccgcaa atcaacatcc cgattttcaa ttggggtgct aacaaagcgg
     7621 ctttggattt gactcgcgta cgcaaagaaa tcagtgtggt gcagtatgag caagcggtac
     7681 aggacgcatt ccaagatgtg tctgatgttt tggttgccaa tgcaaccttg aaacagcaat
     7741 atgcctctca gctcaaaagc accaatgccg aagtggatcg caatcgtttg atgcaaatgc
     7801 gtttcaacca aggcattgct gactctttaa ccatgttgga ttctcagcgc caaagttatg
     7861 cagctcaact ggctttgctt ggtgttcagc aacagtggtt aaacagccgt gtggatttgt
     7921 ataaagtatt gggaggcggc ctgaaagcct ataacgacgg caaagtccaa cgccttttta
     7981 caggcgaata aacagccgta ttgattgctt ttatctaaaa aatgccacct tagcggtggc
     8041 attttctatt atccctgttg caacacaatt tttgctcccg caatttcagc caattgtcct
     8101 aactggtttt gattgagcaa accattgcct aaccgtatca cattggccaa tacaggcaaa
     8161 tggctgtgtt tgactgccgc cgcaatcgct tcaatgtcca aacggtaggg ataacggtgg
     8221 ttgatgctta aggttcctgc ttcacccaaa ataatttgat ggtttccata catggccacg
     8281 cgctcagctg caaccagcca ctgctctaaa gtatggtgtt tatccttaca caacaccacg
     8341 ggcaaattca ggcgacctat ttcttgttgc atactgcgac tgtctaacca ctccccaccc
     8401 aaatacacaa tgtctgcccc tgccaataac acgccatcca attgtctcaa atcacgcacc
     8461 ctagccatta caggctgatt gttgtgatgg ctatccgtca ccacaccaga caaatcgcgt
     8521 aattgttgtt cctcactttt cacccctact tgataagggt tactgggcag ataaaaaggg
     8581 tctagataca caatgcttgc gtttacaggc cgcttaatgt caatgtgtaa catctcttgc
     8641 cccaaagcca caccacggca tacatacaca ctgttttgag gttgggcttc acggctgatg
     8701 atacgccaat cgtgcacaat gcgcaccgca cgctccactt ctggcatttg ttccaacacc
     8761 tgcacattca aaacccgttc atccccaaca gccccaataa tgacacgctc agccccaacc
     8821 gacacatgct catgtaaacc agccgcacgt atgtgctcga tgaccgcatt gattgcctgt
     8881 tcttgggcat gtggttgcat aactattatc atagtgctct ctgaagattt ttattctcgc
     8941 aaattgtgcc attttatagc ccttgttgca gctgttttta agcaaaactt gcataagtgc
     9001 ttaaataggt ttacattcag cgcctgcttt aatcctcaat gcgggtgtag ttcaatggta
     9061 gaacggcagc ttcccaagct gcatacgagg gttcg
//
LOCUS       DAOMVK010000027         9091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_1947_length_9091_cov_3.002213, whole genome shotgun sequence.
ACCESSION   DAOMVK010000027 DAOMVK010000000
VERSION     DAOMVK010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 9091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9091
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1947_length_9091_cov_3.002213"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="PK856_01840"
     CDS             complement(<1..595)
                     /locus_tag="PK856_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRL01144.1"
                     /translation="MSKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRSNQQVDERVMD
                     NGDIEKERGITILAKNTAIDYEGYHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQ
                     EGPMPQTRFVTKKALALGLHPIVVVNKVDKPGARPSWAIDQTFELFDNLGATDEQLDF
                     PIVYASGLSGFAKLTEDETSDTMRPLFETILKHTPEPA"
     gene            773..1708
                     /locus_tag="PK856_01845"
     CDS             773..1708
                     /locus_tag="PK856_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HRL01145.1"
                     /translation="MKILQIGRLSNDVNQHLEREYGALALWQQTQIDEFLQQFGADFE
                     VVVTSAAYGLTAAQIALLPNLRAVCSFGVGYDSIDVAALQQKNIVLSNTPDVLTDCVA
                     DLAMGLMIDVSRGISAGDRYIRKGLWGSKQRMSMQVRVSGKRLGILGLGRIGAAIAGR
                     AIGFNMEIAYHNRSKHPESDYVYLPSVQALAQWADYLVVACPGGAATEHLVDAAVLST
                     LGEHGYLINIARGSIVDEQALVQALDNKQIAGAALDVFEHEPKVSQALMENDSVVLTP
                     HIGSATQETRMEMDKLLLDNVAAFVQEGRLLTAVG"
     gene            1786..2259
                     /locus_tag="PK856_01850"
     CDS             1786..2259
                     /locus_tag="PK856_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01146.1"
                     /translation="MVNLYEILNIPENANVIAIQRAIRLARDRQDIDERHLKLAENYL
                     LDDKRRAQYNKQIGVKTHAGRNGLSVGTDWRYVGGGITLVLVLFIPLMFVMFGQQKAT
                     EQLYQQKDGEYKEAQEAMEKAWGVHQASGETKPQAVERTLDEITDKQNQQLEGME"
     gene            2355..2834
                     /locus_tag="PK856_01855"
     CDS             2355..2834
                     /locus_tag="PK856_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRL01147.1"
                     /translation="MTVENGKVVSFHYTLTNAQGDVLDQSQEHPMPYLHGAGNIIPGL
                     EKELAGKKVGDKLTVNVPAAEAYGEYHEQLVNDVPREAFQGVDQIEPGMQFQANTPEG
                     VQVITVKAVSGETVTVDANHPLAGQDLNFDVEIVEVREATEEESAHGHVHGVGGHHH"
     gene            complement(3144..3614)
                     /locus_tag="PK856_01860"
     CDS             complement(3144..3614)
                     /locus_tag="PK856_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRL01148.1"
                     /translation="MLDQIDLKILRALQANGRLSNQDLADQVALSPSACLRRVKSLEQ
                     AGYIDGYHALLNAAKLGYQVEAFVQVRIDQGNDDWHDQFLKTIANLDEIADAYVITGN
                     ANYLLRIRTRDFEHFSNFIIHTLNRIASVRDIQSNMVMQNLKNNGKLLPLPSSK"
     gene            3786..4829
                     /locus_tag="PK856_01865"
     CDS             3786..4829
                     /locus_tag="PK856_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HRL01149.1"
                     /translation="MSTQRPPVYYAEYLQLDNILNAQQPESAKEGIRADDEMLFIIIH
                     QTYELWFKQILHEINIVREIFLKPNVSDNSPDIYNAVCRLKRVCEILEVLVKQISVLE
                     TMTALDFLDFRDFLRPASGFQSIQFKTIEALLGLEYANRHGQEYYLSQLTEQDRQQVQ
                     DAEKQTSLLVLLGQWLERMPLAEHNQHWSSSHAENHGFWQQFRSAYAADLSAAEAQNL
                     AVFDQLFMQADTYPAERRLSQTASKNALFIMMYRDYPLLQLPYELLSLLLEIDEQLSL
                     WRYRHIHMVGRTIGKRVGTGGSSGAGYLKAAADSHMIFKEIAELTSFLMPRHVLPTLP
                     KAVIAAMSYPSSN"
     gene            4874..6124
                     /gene="kynU"
                     /locus_tag="PK856_01870"
     CDS             4874..6124
                     /gene="kynU"
                     /locus_tag="PK856_01870"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HRL01150.1"
                     /translation="MLTLEYCRQLDAADPLATLKQQFALPENTIYLDGNSLGARPKVA
                     LERATDVVTREWGADLINSWNKNHWWDLPVRIGNKVAKLVGGKDNEVVVTDTTSSNLF
                     KVLATALRIQMEQAPERKIIIAETEAFPTDLYIIQGMAEWLQQGYELQLVAGPEALSA
                     ALNDKTAAVILSHVNYRTGFLWPMQETTAEIHRHGGLIIWDLCHSIGAVDIDLNAADA
                     DFAVGCTYKYLNGGPGAPAMLWVPEKHVNRYGQPLSGWWGHAKPFAMSTNYEPAAGIR
                     RYLSGTQPIVSMSLVECGVDVFLQADMNQIRKKSLALTDLFIALVEQECGQHGLEMIT
                     PREHAIRGSHVSLRHEHGFAIIQALIARGVIGDYREPEVLRFGITPLYLSHEDIWTAV
                     QHLKDVLDTGAWNKAEFHLRGQVT"
     gene            6206..7594
                     /locus_tag="PK856_01875"
     CDS             6206..7594
                     /locus_tag="PK856_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRL01151.1"
                     /translation="MSDFGKIQAREAGLHKKLTAKQMSMIAIGGAIGTGLFLGSKFAI
                     SFAGPGVIVSYAIGGLIALFLMGCLAEMTIAHPTSGSFGAYAEHYISPLAGFIVRYSY
                     WACIVLAVGTEVTAVAEYMKMWFPDVSSWWWIVGFSAALIGVNAYSVKAFGSVEYWFS
                     TIKVFAILAFILLAAVVLFGQHGVSSGVNNLSAHGGFAPNGFWGIWVGVIISIFSYLS
                     IEMIAVAAGEAENPEESVKKAFRATIYRLVIFYLLSLTLIVMLVPWNVLTAKGATSPF
                     ITVMRELGIPYADGILNFIVIIAALSAMNSMLYISTRMMFSLSRAGDAPAFLGKIKEN
                     GVPMNALLLSASGIAVAAIVYTANPDKAFPVMIALSMFGAMVTWGMIFVTHFFFRREV
                     ERTGKQLTFKVPGFPIGTILGACLMLAIMITTYFTNEFKSTMIFGVPFILALVVLFYV
                     MRRNPKAKRLSY"
     gene            complement(7694..8470)
                     /locus_tag="PK856_01880"
     CDS             complement(7694..8470)
                     /locus_tag="PK856_01880"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRL01152.1"
                     /translation="MRLYDNSRTIEDEAAILADFQQRSREAYTHHPHSTHAYGEHPRQ
                     QYDVFTCGKAEAPLLVFIHGGYWQWCDKSDFGFIVNAPLAAGFDVILLEYPLTPEANF
                     TEVVNSIGLALDFLAQKPEYKNRQVHLSGHSAGGHLSAYWQRHPWLNNVCAISGIYDL
                     DPLQQTHLNEALNLSADDIQDFSPMYFPAIKKPLHLVYGGQELAELQWQTTQYAHELS
                     EHSLQVSLKELSACNHYNILDEVFGEQGYWLTHVAYPLAG"
     gene            8599..>9091
                     /locus_tag="PK856_01885"
     CDS             8599..>9091
                     /locus_tag="PK856_01885"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL01153.1"
                     /translation="MKSTMALSSLFTAVALLFTGVGLFIFFSNIELTNAGVSSEKIGL
                     INAGFYLGALLSGVIAQRWVAKVGHIRAFALFTAFITIGILLHVVVQNEIALIVFRVM
                     VGFSDYSLLMVVESWLNARSSNQNRSSILAVYTMIFYMAFMLASIVIATPIESATMYV
                     LCAI"
BASE COUNT         2234 a         1920 c         2371 g         2566 t
ORIGIN      
        1 cagctggttc tggagtgtgt ttcaaaatgg tttcaaacaa tgggcgcatg gtgtcgctgg
       61 tttcgtcttc ggtcaatttg gcaaagccag acaagcctga agcgtaaaca atagggaaat
      121 ccaactgctc atcggtcgca cccaagttgt caaacaattc aaatgtttga tcaatcgccc
      181 agctaggacg agcacctggt ttgtctactt tgtttaccac gacgataggg tgtaagccca
      241 aagccaaggc ttttttggtc acaaagcggg tttgtggcat agggccttct tgtgcgtcaa
      301 cgagcaatac cacgcagtca accatgccca atacacgttc tacttcacca ccgaagtccg
      361 cgtgtcctgg cgtgtcgacg atgttgatgt ggtaaccttc gtagtcaatg gcagtgtttt
      421 ttgccaaaat ggtgatgcca cgttcttttt cgatgtcgcc attgtccatg acgcgttcgt
      481 cgacttgttg gttagaacgg aaagtgcccg attgacgcaa taattggtca accaatgtgg
      541 ttttgccatg atcgacgtgg gcgatgatgg cgatgttgcg aatttgtttg ctcataatct
      601 cgttgatatc taatcgtttt tttgtaacgg cgcattataa caccattcaa aaaaccatga
      661 cggcttttct attgcaatgc agcaagtgga ttaagcggcc tgtaaattct gttcgggagg
      721 cgagtggaat gcttttgatg gtgtcattta gccgaaattt aaggaagttg cgatgaaaat
      781 cttgcaaatt ggtcgattgt ccaatgatgt gaatcagcat ttggagcgtg aatatggcgc
      841 acttgccttg tggcaacaaa cacaaatcga cgaattttta caacagtttg gtgcggattt
      901 tgaggtggtg gtgacttcgg cagcttatgg tttgactgcc gctcaaatag ccttgctacc
      961 gaatttgcgt gcggtgtgca gttttggcgt tggttatgat tcaattgatg tggctgcact
     1021 gcagcaaaaa aacatcgtat tgagcaatac cccagacgtg ttgactgatt gtgtggccga
     1081 tttggccatg ggtttaatga tagatgtctc tcgcggcatc agtgcgggtg accgttatat
     1141 ccgtaagggc ttgtggggca gcaagcaacg tatgagcatg caagtgcgtg tcagtggaaa
     1201 gcgcttaggg atattgggtc tggggcgaat aggtgctgcg atagcagggc gggcgatcgg
     1261 cttcaatatg gaaattgcct atcacaaccg ttccaaacac cctgaatctg attatgtgta
     1321 tctgcccagt gtgcaggctt tggctcagtg ggccgattat ttggtggtgg cgtgtcctgg
     1381 tggggcagca accgaacatt tggtggatgc cgctgtttta agtactttgg gtgagcatgg
     1441 ttatttgatt aacattgccc gtggctcaat agtggacgag caagctttgg ttcaggcttt
     1501 ggataacaag caaattgcag gtgcggcatt ggatgtgttt gagcatgagc ccaaggtgtc
     1561 gcaagccttg atggaaaatg attctgtggt gttgacgccg cacatcggca gcgcaaccca
     1621 agaaacacgc atggagatgg ataagttact gttggacaat gtcgcggctt tcgtgcaaga
     1681 agggcgtttg ctcactgcgg ttggctaaaa ataaattcag tggttggtgt ggtatttaca
     1741 ggccgcttca taaccatgat ttgaacatta cacgaggaca ccaatatggt gaatttgtac
     1801 gagattttaa acatacccga aaacgccaat gtgattgcca tacaaagggc aatacgtttg
     1861 gcacgggatc gccaagacat agatgaacgg catttgaaat tggcagaaaa ctatttgctc
     1921 gatgacaaac gccgtgcaca gtataacaag caaatcggtg tgaaaaccca tgctggacgc
     1981 aatggtttgt cggtaggcac ggattggcgc tatgttggcg gtggtattac tttggtactg
     2041 gttttgttca tcccgttgat gtttgtgatg tttggtcaac aaaaagccac agagcagttg
     2101 taccagcaaa aagacggtga atacaaagag gcgcaagagg caatggaaaa ggcttggggc
     2161 gtgcaccaag ccagtggtga aaccaagcca caagcggtag agcgaacttt ggatgaaatc
     2221 accgataagc aaaatcagca attggaaggt atggagtagt acaataattt gagccttagg
     2281 cttgaaatct cgcgtttggt gcttatttag aaacaaggta agatgcccaa acatttaacg
     2341 tgaaggaaca cataatgaca gttgaaaacg gcaaagtcgt aagttttcat tacactttaa
     2401 ccaatgctca aggcgatgta ttggaccaat ctcaagaaca tcctatgcct tatttgcatg
     2461 gcgctggcaa catcattcca ggtttggaaa aagaattggc tggcaaaaaa gtgggcgaca
     2521 aattaaccgt taacgtgcct gctgctgaag cttacggcga gtaccacgaa caattggtaa
     2581 acgatgtgcc gcgtgaagcc ttccaaggtg tggaccaaat tgagcctggt atgcaattcc
     2641 aagccaacac ccctgaaggt gtgcaagtga tcactgttaa agccgtgagt ggcgaaaccg
     2701 tgactgtgga tgcaaaccat cctttggcgg gtcaagattt gaactttgat gtggaaatcg
     2761 ttgaagtgcg cgaagccact gaagaagaat ctgctcatgg tcatgtacac ggcgttggtg
     2821 gtcatcacca ctaagccttg ctgatagcgt tttgaaaagc ggcctgtaac ttacaggccg
     2881 cttttttgtg tatcttgtat tcaatattgt tggttcttgt ttagattgtg caaggctgca
     2941 gcaggattac ttgactgtcc ataactattc gtaactattc atattctggt aatttttgta
     3001 gaggccatca ccctcagtcc tgagtatgta atcccacttt ggatacgggt ttaacaaggt
     3061 gtttgctacc gtgttcgatt tcaaaccagt gactaactcg catctttgtt tacaggccgc
     3121 cttggctatt gattgtctat tgtttatttg ctgctgggta gaggcaataa tttgccgttg
     3181 ttcttcaggt tttgcatcac catattggat tgaatgtcgc gcacactggc gatgcgattc
     3241 aaggtatgga tgatgaaatt ggaaaagtgt tcaaaatcgc gggtgcgtat gcgcagcaaa
     3301 tagttggcat tgcctgtaat cacataggca tcggcaatct catccaagtt cgctatggtt
     3361 ttgagaaatt ggtcgtgcca atcatcgttg ccttggtcta tgcgcacttg cacaaaagcc
     3421 tcgacttgat agcccagttt ggccgcattc agaagagcat gatagccatc aatataaccc
     3481 gcttgttcca ggcttttgac ccgccgcaaa caggcagaag gcgacaaggc gacttggtcg
     3541 gccaaatctt ggttgctcaa gcgaccattg gcttgtaagg cgcgcaaaat tttcaaatca
     3601 atttggtcta acatcgtcat tcctgcggtt tttagtgcgt aaagcagaat attatttgaa
     3661 aactgatgtt tttgctgcgt tattatgcaa ttttctgcat atgcccatgt gttacactgc
     3721 tgtaatccta aggcccaatc aggccaaaac acgatgtatt ctttccaaat agagccaatg
     3781 acaccatgag tacacaacgc ccgcccgttt attatgccga gtatttgcag ctggataaca
     3841 tactcaatgc ccaacagcct gaaagtgcca aagagggcat acgcgctgat gacgaaatgt
     3901 tgttcatcat cattcaccaa acctatgagt tgtggttcaa gcaaatattg catgaaatca
     3961 atattgtgcg ggaaattttc ctgaaaccca atgtatcgga caactctccc gatatataca
     4021 atgcggtgtg tcgtctgaag cgggtgtgcg aaattctgga agtgttggtg aagcaaatat
     4081 cggtgttgga aaccatgacc gcactggatt ttttggattt ccgcgacttt ttgcgtcctg
     4141 cctctggttt tcaaagcata cagtttaaaa ccattgaagc cttattgggt ttggaatatg
     4201 ccaatcgcca tggtcaagaa tactatttgt cacaattgac cgagcaagac agacaacaag
     4261 tgcaagacgc tgagaagcaa acctcattgt tggttttgct aggacagtgg ttagaacgca
     4321 tgcctttggc cgagcacaat cagcattgga gcagcagtca tgctgaaaac catggctttt
     4381 ggcagcagtt tcgcagtgca tacgccgccg atttgtctgc tgcggaagcg cagaatttgg
     4441 cggtgtttga ccaattgttt atgcaggccg atacctatcc cgctgaacgc cgtttgagcc
     4501 aaaccgcgag caagaatgct ttgttcatca tgatgtaccg tgattatcct ttattacaat
     4561 tgccgtatga gttgctcagt ttgttattgg aaattgatga gcaattgtca ttgtggcgct
     4621 accgtcacat ccacatggtc ggccgtacca taggcaaacg cgtgggtaca ggcggcagtt
     4681 ctggcgcggg ttatttaaaa gccgcagcag acagccacat gatattcaaa gaaattgcgg
     4741 aattgacttc gtttttgatg ccaagacatg ttttgccgac tttgccaaaa gcagttatcg
     4801 cggccatgtc ttatccttct tcaaattagt ttctaaggtt tcattcaatc aaaatcaaca
     4861 taggagaaac accatgttaa cgctagagta ttgccgtcaa ttggacgcgg ctgacccatt
     4921 ggccacgtta aagcaacaat ttgccttgcc tgaaaacacg atttatttgg atggtaattc
     4981 actgggggct cgccctaagg tggctttgga gcgagccacc gatgtggtga cccgtgagtg
     5041 gggcgctgat ttgattaaca gttggaacaa aaaccattgg tgggatttgc ccgtgcgcat
     5101 tggtaacaaa gttgccaagt tggtgggcgg gaaagacaac gaggtcgttg tcaccgacac
     5161 caccagttct aacctgttca aggttttggc aacggcgttg cgcattcaaa tggaacaagc
     5221 cccagagcgc aaaatcatca tcgccgaaac cgaagccttt cctaccgatt tgtacatcat
     5281 tcaaggcatg gcggaatggt tgcaacaagg gtatgagttg caattggtgg caggtcctga
     5341 agcgttatca gcggccttga atgacaaaac cgcagcggtg attttgtcgc atgtgaatta
     5401 ccgcactggc tttttgtggc ccatgcaaga aaccacggca gaaattcacc gccatggtgg
     5461 tttgattatt tgggacttgt gccattcgat aggtgcggta gacatagatt tgaatgccgc
     5521 agatgccgat ttcgccgttg gttgtactta taaatatttg aatggtggcc ctggtgctcc
     5581 tgccatgtta tgggtgcctg aaaaacatgt gaatcgttac gggcaacctt tgtctggttg
     5641 gtggggacat gccaagccgt ttgcaatgtc aacaaactac gaacccgcag caggcatacg
     5701 ccgttacctc agtggtactc agcctattgt gtccatgtct ttggttgaat gtggtgtcga
     5761 tgtgttttta caggccgata tgaaccaaat ccgcaaaaaa tcattggctt tgaccgattt
     5821 gttcattgca ttggtagagc aagaatgtgg tcaacatggc ttggaaatga ttacgccacg
     5881 tgagcatgcg atacgtggta gccatgtgag tttgcgccat gaacatggtt ttgccatcat
     5941 tcaggccttg attgcgcgag gtgtgattgg tgattaccgt gagcctgagg ttttgcgctt
     6001 tggcattacg cctttgtatt taagccatga agacatttgg acagcggtac aacacttgaa
     6061 agatgtgttg gatacggggg cttggaacaa ggctgagttt catttgcgtg gccaagtgac
     6121 ttaaacaccc gcgttgccga tgcctgtttg ggcatcggca gtttgtacac aacataatca
     6181 taaaattaaa atagagagaa acagcatgag cgattttggc aagatacagg cgcgtgaggc
     6241 aggcctacac aaaaaactca cggccaagca aatgagcatg attgccatcg gtggggcaat
     6301 tggcacgggt ttgttcttag gcagcaaatt tgccatcagt tttgcaggcc ctggcgtgat
     6361 agtcagttat gccataggag gcttgattgc tttgttcttg atgggttgtt tggcggagat
     6421 gaccattgcc caccctacgt ctggctcgtt tggggcgtat gccgagcatt acatcagtcc
     6481 attggcgggt tttatcgtgc gctacagtta ttgggcctgt attgtgttgg cggtgggcac
     6541 tgaggtgacg gcagtcgcgg agtacatgaa aatgtggttc cccgatgtca gttcatggtg
     6601 gtggattgtg ggcttttctg ctgccttgat aggggtgaat gcctacagcg tgaaggcgtt
     6661 tggttcggtt gagtattggt tttctaccat caaagtgttc gcgattctgg cctttatctt
     6721 gttggcggcg gtggtgttgt ttgggcagca tggtgtcagc agtggtgtga acaatttgtc
     6781 tgctcacggt gggtttgcac cgaatggttt ttggggcatt tgggtgggtg tcatcatctc
     6841 gattttcagt tatttgagca ttgaaatgat tgctgtggca gcaggtgagg cggaaaaccc
     6901 tgaagagtca gtgaagaaag cctttagagc caccatttat cgcttggtga ttttctactt
     6961 gttgtcgtta actttgattg tgatgttggt gccttggaat gtgttgactg ccaaaggtgc
     7021 gaccagccct tttattacgg tgatgcgtga attgggcata ccttacgccg atggtattct
     7081 gaattttatc gtcatcatcg cagccctgtc ggcgatgaat tccatgctct acatttccac
     7141 acgcatgatg ttcagcttgt cacgagcggg tgatgcgcct gcgttcttgg gtaaaatcaa
     7201 ggaaaatggc gtgcccatga atgccttgtt gctgtctgcc agtggcattg ctgtggcggc
     7261 aattgtttac accgccaatc ctgataaagc cttccctgtg atgatagcgc tgtcgatgtt
     7321 tggcgcaatg gtgacatggg gcatgatatt tgtgacccat ttcttcttcc gccgcgaagt
     7381 ggagcgcaca ggcaaacagc tgacattcaa agtacccggt ttccccatag gtacgatact
     7441 gggcgcatgt ttgatgttgg cgattatgat caccacttac ttcaccaatg aattcaaatc
     7501 cacgatgatt tttggtgtgc cgtttatttt ggctttggtg gtgttgtttt atgtgatgcg
     7561 tcgcaatccc aaggcaaaaa gactcagtta ttaagcttta ctgatgttga atgagtttcc
     7621 acaaatgttt acaggccgct tctaagcggc ctgtaaacat ttaagtcgaa tcaattgcaa
     7681 agaatataag gctttatcct gccagcggat acgccacatg agtgagccaa taaccttgct
     7741 caccaaacac ctcatccaaa atattgtaat gattgcaagc actcaattct tttaagctca
     7801 cttggagtga gtgctcactt agctcatgcg catactgggt ggtttgccat tgcagctcgg
     7861 ccaactcctg cccgccatac accaaatgca gtggcttctt gatggcgggg aaatacatgg
     7921 ggctgaaatc ttgaatgtca tcagcgctca ggtttaaggc ctcattcaaa tgtgtttgct
     7981 gcaatgggtc taaatcgtaa atgccgctga tggcacagac attgttcaac caaggatggc
     8041 gctgccaata agcgctcaga tgaccgcccg cagagtggcc gcttaaatgt acttggcgat
     8101 ttttatactc tggtttctgc gccaagaagt ccaaggccaa gcctatgctg ttcaccactt
     8161 cggtaaagtt ggcttcaggg gtgagggggt attccaggag tatgacatca aagcctgctg
     8221 ccaatggggc attgactatg aaaccaaaat cgctcttgtc acaccattgc caatagccac
     8281 catgtatgaa caccaacagc ggtgcttcgg ctttcccgca ggtgaacaca tcgtattgtt
     8341 ggcgtgggtg ttcgccatag gcatgggttg aatggggatg gtgtgtataa gcttcgcggc
     8401 tgcgctgttg aaagtcagcc agaatcgccg cttcgtcctc tatggtgcgg ctgttgtcgt
     8461 ataaacgcat agtttatcca ggtggcaaaa ttattcttgt taagtgatgg ggttttctct
     8521 gtatttctgt gataatccag ctgtcttgca gactcaattt aaacgccaga tgtacctaaa
     8581 tgatattgga attaacacat gaaatccacc atggctttgt cttcactctt cactgctgtt
     8641 gcattgttgt tcacaggcgt tggtctgttc atcttttttt ccaatataga attaaccaat
     8701 gccggcgtca gctctgaaaa aatcggcctg attaacgcgg gtttttattt gggcgctttg
     8761 ctcagtggcg tcatcgcgca gcgttgggtg gccaaagtgg ggcacatccg cgccttcgcc
     8821 ttgtttactg ccttcatcac cataggcatc ttgttgcatg tggtggtgca aaatgaaatc
     8881 gccttgattg tgtttcgggt gatggtcggt ttcagcgatt acagcctgct gatggtggtg
     8941 gagagctggc tcaatgcccg cagcagcaac caaaaccgca gcagcatttt ggcggtgtac
     9001 acgatgatat tctacatggc tttcatgctc gcctcgattg tgattgccac gccgattgaa
     9061 agcgcgacca tgtatgtgtt gtgcgcgatt t
//
LOCUS       DAOMVK010000028         8930 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_2003_length_8930_cov_4.058479, whole genome shotgun sequence.
ACCESSION   DAOMVK010000028 DAOMVK010000000
VERSION     DAOMVK010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 8930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8930
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2003_length_8930_cov_4.058479"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PK856_01890"
     CDS             complement(<1..309)
                     /locus_tag="PK856_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRL01154.1"
                     /translation="MLFTSAVRYSTAALVLGLGLSACGGQSTDTAKASSSAPAESASG
                     ATTASGKTYKIALNAEFAPFESITPDGKMEGFDIDLMDAMAKAGGFQVEYKHQPWEGL
                     F"
     gene            complement(589..2778)
                     /locus_tag="PK856_01895"
     CDS             complement(589..2778)
                     /locus_tag="PK856_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRL01155.1"
                     /translation="MDTSLLKGLNAEQLAAVTWPPSAALVLAGAGSGKTRVLTTRIAW
                     LLDTEQASVHSIMAVTFTNKAAKEMQHRLGEMVPYSTRAMWLGTFHGLCHRFLRIHYQ
                     DAGLPSAFQILDSGDQLSVIKRLLKGLNIADEVLAPKSLQYFINNCKENGQRASVLTA
                     NDVTMRQQVQCYQAYDDLCQQEGLVDFAELMLRSYEVMQAHAHIRLHYQQRFNHILVD
                     EFQDTNNLQYRWLKLFAGDSAAVFAVGDDDQSIYAFRGANVGNMSALMRDFGIDAPIK
                     LEVNYRSVGHILTAANTLIANNEQRLGKNLRLPDESADAHGEKLRFYWANTDFLEADF
                     VVKEAKARVQDGVAFDNVAVLYRSNAQSRVLEQALFAANVPYKIYGGLRFYERQEIKH
                     ALAYLRMVVHPQDDNAVLRIINMPPRGIGARSIEAVQAVAQDLQTSLWEALLHLATNP
                     KNSKLIAFVNLIQSLQTQAQGLDLAETVQAALNLSGLVEHYQNAKGDNQERIDNLQEL
                     ISAAMAFKPDEVVLDEWASPETETLPLVAFLSNASLEAGERQAGEGEAAMQLMTVHAA
                     KGLEFDTVFLTGLEEGLFPSDYSLQERGGLEEERRLMYVAITRARQRLYMSAAQQRLL
                     HGQSHYAMVSRFVQEIPEAVVQSLSSGLKRAQPSLRTREEPLIKAKSKQDSFAGFALG
                     QNVRHEKFGVGVIIDATHKGEHANLKINFGKNGVKELSTQFAKLESV"
     gene            complement(2880..3317)
                     /locus_tag="PK856_01900"
     CDS             complement(2880..3317)
                     /locus_tag="PK856_01900"
                     /inference="COORDINATES: protein motif:HMM:NF023204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HRL01156.1"
                     /translation="MKANLWGMLLCAVALNTPAETVWSITSSPSQPSQQGHKPHRPYP
                     DRPRPPHHHHPAPPYYPYYPPYAYPNQTVVVQQPLEPVAIENLRVGDRMPPDYRYSDY
                     AVDDYRGHGLNKPASHQRWALIAGQYYLYDLKSWQIKDIVAKR"
     gene            3638..4789
                     /gene="argE"
                     /locus_tag="PK856_01905"
     CDS             3638..4789
                     /gene="argE"
                     /locus_tag="PK856_01905"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009115278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HRL01157.1"
                     /translation="MNTVDWLSRLIAFDTTSRYSNVQLIDCVDEYLRGLGLQPWRSWN
                     AEQSKANLFVTLPAHNGNITGGTVLSGHTDVVPVDGQQWTSNPFQAVIRDGKLYGRGA
                     CDMKGFIAACLNMVPLALQTKLREPIHLALSYDEEIGCLGAPVMLEQLQQHGLSPKQC
                     IVGEPTSMQMVVAHKGINVFSCKVHGHSAHSSLTPQGVNAIDYAARIIVFLQGLGKQL
                     QTQHEQDAAFDVPFSTLNVGRIRGGTAVNIIPNECELQFDYRNLPTTNTDAVLLPLKA
                     YLQELIQEMQQVHGDATIVLKQLEQVPAMQSVEGNALYTLIEALVDEHKRSKVAYATE
                     GGHFQLAHMDTVICGPGSIVQAHKADEYIALSQLERCDRFLQQLLQTFS"
     gene            4806..5501
                     /locus_tag="PK856_01910"
     CDS             4806..5501
                     /locus_tag="PK856_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - oxidoreductase activity, acting
                     on NAD(P)H, nitrogenous group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HRL01158.1"
                     /translation="MDHTAVDQAITGRHSVRAFTSQAVDKATLLELLNVAARAPSGTN
                     CQPWKVYVATGAARDRIVTEVCAIHDAARHDKELAAQYTEAYPYYPEEWISPYIDRRR
                     ENGWGLYGLLGIEKGQKDKMHEQHQRNFKLFDAPVALFFTVNKVMGIGSKMDIAMMMQ
                     NLMVAAQARGLATCPQAAWNHYHKIVFPIVGAGDDEEMVCGMALGYADETELVNKYIT
                     PRVKAEEFTHFVD"
     gene            5702..5974
                     /locus_tag="PK856_01915"
     CDS             5702..5974
                     /locus_tag="PK856_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01159.1"
                     /translation="MKKFALSSAVAVLALTTSLANAAVGVNHNGPQLNPYAQSNAQVQ
                     GNEVVLELVSNDARLNSEAINPLYKVVSPATTGSAEGQAYSVRVSH"
     gene            complement(6042..6620)
                     /locus_tag="PK856_01920"
     CDS             complement(6042..6620)
                     /locus_tag="PK856_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HRL01160.1"
                     /translation="MDAFLYSTAAVALAEVGDKTQLLTLFLAARFRNKWAIIAGIFVA
                     TVINHFVSGWLGAWAGSAFNENLLAWIVGISFVVVGLWLLVPDKEDEEDGKWLAYGAF
                     FATTVLFFLAEIGDKTQIATVLLAAKYQAVFWVVAGSTLGLMIANVPMVFLGDWLLKK
                     LPLQVIRIAACVLFVVLGLITLYQPVLALFAQ"
     gene            complement(6953..7705)
                     /locus_tag="PK856_01925"
     CDS             complement(6953..7705)
                     /locus_tag="PK856_01925"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRL01161.1"
                     /translation="MEQQQIFAVLKQQLHAQGKTYADLAKHLDVSESSIKRLFANQSV
                     TLERLLEICQYLQLNFAELARLVDEQRPVLDQLTLAQEKQLMQDPKLILVAVCMMNHW
                     PIEAVIEHYQVDLPECILKLTQLDKMGMLQLLPNNRVRLRVSQQFNWQPNGPIQRYIE
                     EQGIGDFFDAHSDEEGHEEILFGHGMLSNDSVHAMRTVLKKCQQQMAQAHRQSLPVPQ
                     SQKRGMAMVLALRTWEPKWFRQLRRVEKQIDK"
     gene            7839..8624
                     /locus_tag="PK856_01930"
     CDS             7839..8624
                     /locus_tag="PK856_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01162.1"
                     /translation="MSASSPLNASPSWALLATITLALLATGHINQLAHYHDFADQTHW
                     LMLPNGKDVWSNMGLILIGLWACYGLKRAHIPFTGRISAYVLASAIVLTGLGSSYYHL
                     QPNDFSLIWDRLPISLICAGLLGMVFQYTHQRSDGIVLSIAIIFAVLGVWYWHVSGDL
                     RLYLGMQVFTILALPLWLWQARAPRAITLALLWAIGFYIASKVTEALDVQLFALSSHI
                     ISGHTLKHLFATMAMACVVVAMRAMVLTPISASGVRCLNRKFG"
     gene            complement(8581..>8930)
                     /locus_tag="PK856_01935"
     CDS             complement(8581..>8930)
                     /locus_tag="PK856_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HRL01163.1"
                     /translation="KPLTEAQTEQVTQAWPAAKTFIIRSSDKVSPLLRGNGRNKLAVR
                     VPAHDDARLLSKLAASPLVSTSCNRSGKRFCTTTREVKRQFGRQVMVVTGRIGSNKQP
                     SQIYDLDSGRRLR"
BASE COUNT         2229 a         2388 c         2114 g         2199 t
ORIGIN      
        1 gaacaaacct tcccaaggtt ggtgtttgta ttccacttgg aagccaccgg ctttggccat
       61 ggcgtccatc aaatcaatgt caaagccttc cattttgcca tcaggagtga tggattcaaa
      121 cggggcaaat tcggcattca aggcgatttt ataagtcttg ccagatgccg tagtagcacc
      181 actggcggat tcagcaggag cactgctgct ggctttggcc gtgtcagtag attgaccacc
      241 acaagcactc aaacccaagc ccaaaaccaa cgcggcagtg ctgtaacgaa cagcagacgt
      301 aaacaacata attcactcca taaaaaacaa aacgccaacc ctcgggtcag ccagatgcta
      361 cgcattataa aacaatgcac tgcacaatgg ggataaaatg tgacttcatc tccttgattc
      421 ggcaatacaa accactctaa atttcaccag cactcttcag gcatgcaagc ggcctgtaaa
      481 cccattccaa ccctgtttac aggccgcttg ttgaaacacc ccataacaaa gggctgcaca
      541 accatgattg atgcagccct ttttcgtgtc accagatggc gacaggattc acacgctttc
      601 caatttggca aattgtgtgc tcaattcctt cacgccgttt ttgccaaaat tgattttcaa
      661 attggcgtgc tcgcctttgt gggtggcgtc gataatcacg cccacgccaa atttttcatg
      721 gcgcacgttt tgccccaagg caaagcctgc aaaactgtct tgtttgcttt tcgctttaat
      781 caggggttct tcacgggttc tgaggctggg ctgggcacgt ttcaggccgc ttgaaagcga
      841 ctgcaccacg gcttcgggaa tctcttgcac aaagcgcgac accatcgcgt aatggctttg
      901 cccatgcaac aaacgctgtt gtgccgcgct catgtacaag cgttggcgcg cacgcgtaat
      961 cgccacatac atcaaacgtc gctcttcttc caagccaccg cgctcttgca aggaataatc
     1021 gcttgggaat aagccttctt ccagacccgt taaaaacacc gtgtcaaatt ccaaaccctt
     1081 ggctgcatgc accgtcatca attgcatcgc cgcttcgcct tcacccgctt ggcgctcgcc
     1141 tgcctccaaa gaagcgttgc tgagaaacgc caccaagggc aaagtctcgg tttcggggct
     1201 ggcccattca tccaacacca cctcatcggg cttgaacgcc atggccgcac tgatgagctc
     1261 ttgcaagttg tcgatgcgct cttggttgtc gcctttggca ttttgataat gctcaaccaa
     1321 gccactcaga ttcaaagcgg cctgtacggt ttctgccaaa tccaagcctt gcgcttgggt
     1381 ttgcaggctt tgtatcaggt tcacaaacgc tatcaatttg ctgtttttgg ggttggtagc
     1441 caagtgcaac aaagcttccc acaaactggt ttgcaaatcc tgcgccaccg cttgcactgc
     1501 ctcaatgctg cgcgcaccga tgccgcgcgg tggcatgttg atgatgcgca ataccgcatt
     1561 gtcgtcttgc ggatgcacga ccatgcgcaa atacgccagc gcgtgtttga tttcttggcg
     1621 ctcgtaaaag cgcaagccgc cgtaaatttt atacggcacg ttcgctgcaa acagcgcttg
     1681 ctccaacacc cgcgattgcg cattgctgcg gtataacacc gccacattgt caaaagccac
     1741 gccatcttgc acgcgcgcct tagcttcttt caccacaaaa tcggcctcga gaaagtcggt
     1801 attggcccaa taaaagcgca atttttcacc gtgtgcatcg gcagactcat cgggcaaacg
     1861 caaatttttg cccaaacgct gttcattgtt ggcgattaag gtgttggcgg cagtcaaaat
     1921 atgccctacc gagcggtaat tcacttccaa tttgattggc gcatctatgc caaagtcgcg
     1981 catcaaggcg ctcatattgc cgacattggc gccgcgaaac gcgtaaatgc tttggtcgtc
     2041 gtcgcccaca gcaaacaccg ccgcgctgtc gcctgcaaac aatttgagcc agcggtattg
     2101 caaattgttg gtgtcttgaa actcgtccac caaaatgtga ttgaaacgct gttggtaatg
     2161 caaacgaatg tgcgcatggg cttgcatcac ctcatagctg cgcagcatca attcggcaaa
     2221 atccaccaag ccttcttgtt ggcacaaatc atcgtaagcc tgataacact gcacttgttg
     2281 gcgcatggtc acatcattgg ccgtcaacac actggcgcgt tggccgtttt ctttgcagtt
     2341 gttgatgaag tattgcaagg atttcggtgc caacacttca tcggcgatgt tcagcccttt
     2401 gagcaaacgc ttaatcaccg acaattggtc gccgctgtcg agaatttgaa acgcactggg
     2461 caagcccgcg tcttggtaat ggatgcgcaa aaagcggtgg cacagaccgt gaaacgtgcc
     2521 caaccacatc gcgcgcgtgg aatacggcac catctcaccc aagcggtgtt gcatttcttt
     2581 ggcggctttg ttggtaaacg tcaccgccat gatgctgtgc acactggctt gctcggtatc
     2641 caaaagccac gcgatgcgcg tggtcaacac acgggttttg ccactgcccg caccagcgag
     2701 caccaatgca gcagacggcg gccaagtcac ggcagccaat tgctcggcat tcaagccttt
     2761 gagtaaagaa gtatccatgg cggtattgta acggtttcca agtgggcgcg gaatgtcaat
     2821 gcggcctgta aacgatttac aggccgcaca tgatggctta ttgctttgct agcatcacat
     2881 taccgcttgg caacaatgtc tttgatttgc caactcttta aatcgtacaa ataatactgc
     2941 cctgcaatca aggcccagcg ctgatgtgat gcgggtttgt tcaaaccatg accgcgataa
     3001 tcgtctaccg cgtaatcgct gtaacgatag tcaggcggca tgcggtcgcc cacgcgcaaa
     3061 ttttcgatgg ccacaggttc aagcggttgt tgcaccacca cagtttggtt tggatacgca
     3121 tacggtgggt aatacggata atacggtggt gcgggatggt gatggtgcgg tgggcgcggt
     3181 ctatcagggt aaggccgatg tggcttatgc ccttgctgtg agggttgcga aggagagctg
     3241 gtaatcgacc acacggtttc agcaggcgtg ttcagtgcca ccgcacacaa caacatgccc
     3301 cataaatttg ctttcatttc acccctcttt tcttcaaatc tgtcactacc caccatataa
     3361 gcacgatata ggtgattgca acagttaatc caagtaaaac cgctgtttca cggctcaaac
     3421 ggtgaacatg cagcctgtaa accgccaata gacttcatct acatctattg tggtgtgccc
     3481 gtaccatgat ttcacgtgaa acatttgaat gccaacaacg taatgtccat ctgtttgact
     3541 aaaacacttg ctcaagccgt attacaggcc gcttggtaca cgagttttga tggtttgtgg
     3601 taaggtgaat gcttcttgtt cacactgagc cgccaccatg aatacagtag attggttaag
     3661 ccgattaatc gctttcgaca ccaccagccg ttattccaat gtgcagctga ttgattgcgt
     3721 tgatgagtat ttgcgcggtt tgggtttgca gccgtggcgc tcttggaacg cagaacaaag
     3781 caaagccaat ttatttgtga ccttgcccgc ccacaatggc aacatcactg gtggcacagt
     3841 gttatcaggc cacaccgatg tggtgcctgt ggatggacaa cagtggacga gcaatccttt
     3901 tcaggccgtc attcgcgatg gcaagctgta tggccgcggt gcttgtgaca tgaaaggctt
     3961 tatcgcggcc tgtttgaaca tggtgccact ggctttgcaa accaagctgc gcgagccgat
     4021 acacttggca ctcagttacg atgaggaaat tggctgtttg ggcgcgcccg tgatgctgga
     4081 acaattgcaa caacacggtc tgtcccccaa gcaatgcatt gtgggcgagc ccaccagcat
     4141 gcaaatggtg gtggcgcaca aaggcatcaa tgtgttttcc tgcaaagtac atggccattc
     4201 cgcccattct tcgctcaccc cacaaggtgt gaacgccatt gattacgccg cccgcatcat
     4261 tgtctttttg caaggcttgg gcaaacaatt gcaaacccag catgaacaag atgcggcgtt
     4321 tgatgtgcca ttcagcacgc tgaatgtcgg tagaatacgc ggtggtacgg cggtgaacat
     4381 cattcccaac gaatgtgaat tgcagtttga ttaccgcaat ttgcccacca cgaacaccga
     4441 tgctgtgttg ttgccgctca aagcctatct gcaagaattg atacaagaaa tgcagcaagt
     4501 gcatggcgat gccaccatcg tgctcaagca attggaacaa gtgcccgcaa tgcaaagcgt
     4561 ggaaggcaat gccttgtaca cattgattga ggctttggtg gatgaacaca aacgcagcaa
     4621 agttgcctat gccaccgaag gtggccattt tcaattggcc catatggaca cggtgatttg
     4681 tggcccaggc agcattgttc aagcacataa agccgacgaa tacattgcct tgtcgcagct
     4741 tgagcgttgc gaccgctttt tacagcaatt gttacaaacc tttagttgag attttaggaa
     4801 ttcgcatgga tcacacagca gtagaccaag ccattacagg ccgccattca gtacgtgcct
     4861 tcacctccca agccgtggac aaagccaccc tattggaatt gttgaacgtt gccgcccgcg
     4921 ccccatcagg caccaattgc caaccttgga aggtgtatgt ggctacgggt gccgcgcgtg
     4981 accgcatcgt cactgaagtg tgtgccatcc acgatgcggc tcgccatgac aaagaattgg
     5041 ccgcccaata caccgaagcc tatccctatt acccagaaga atggatttca ccttacatcg
     5101 accgccgccg tgaaaatggc tggggcttgt atggcttgct cggcatagaa aaaggccaaa
     5161 aagacaaaat gcacgaacaa caccaacgca atttcaagtt gtttgatgcg cctgtcgcct
     5221 tgtttttcac cgtcaacaaa gtcatgggca taggctcgaa aatggacatt gccatgatga
     5281 tgcaaaacct gatggtggcc gcccaagcgc gcggcttggc cacttgccca caagccgcgt
     5341 ggaaccacta tcacaaaatc gtgtttccca tcgtcggcgc aggcgacgat gaagaaatgg
     5401 tgtgtggcat ggctttgggt tatgccgacg aaaccgaatt ggtgaacaaa tacattaccc
     5461 ccagagtgaa agcggaagag ttcacccatt ttgtggattg aagcacacca agcttgtttt
     5521 ctttttttac aggacgctga atcagcgtcc tatttttgtt actgcttcac caaccaccat
     5581 catttcgctt taattattaa cttaaaatca acaatcattt aattaaaccg ctcttactgt
     5641 acgaattaat aaacattaca ctgtgtacac attaagaaaa acagctttaa aggtaaacac
     5701 aatgaaaaaa ttcgcattga gcagtgcagt agccgtattg gctttgacta cttccttggc
     5761 caacgccgcc gtgggcgtga accacaatgg cccacaatta aatccctatg cccaaagcaa
     5821 tgcccaagtt caaggcaatg aagtggtatt ggaattggtg tccaacgatg cccgattgaa
     5881 ctcagaagcc atcaatccct tgtacaaagt ggtgagccca gccaccacag gctcggctga
     5941 aggccaagcc tatagcgtac gtgtcagcca ctaagatgcc atgcaaaata ggcctgtaaa
     6001 ccctttaaaa ggtttacagg ccgctttgtt tatataactg cttactgcgc aaacaatgcc
     6061 aacacaggtt ggtataaggt aatcaagccc aacaccacaa acaacacaca cgctgcgatg
     6121 cggatgactt gcaaaggcaa ttttttcaat agccaatcgc ccaaaaacac catgggcacg
     6181 ttggctatca tcaagcccaa ggttgagccc gccaccaccc aaaacaccgc ttggtatttc
     6241 gctgccaaca acaccgttgc gatttgggtt ttgtcaccga tttccgccaa gaaaaacaat
     6301 acggttgtcg cgaaaaaagc accgtaagcc agccatttgc cgtcttcctc gtcttcttta
     6361 tcgggcacca acagccacaa accgaccacc acaaagctga tgccaacaat ccaagccaac
     6421 aggttttcat tgaaagccga gcccgcccaa gcacccaacc agcccgatac aaaatggttg
     6481 ataacggtgg ccacgaaaat gccagcaatg atcgcccatt tattgcgaaa acgcgccgcc
     6541 aaaaacaaag tcaacaattg ggttttgtcg cccacttcag ccagcgcaac ggcggcagtg
     6601 gaatagagaa atgcgtccat agaatgaggt tccgtttgtg ggctgatttt gtgtacaaaa
     6661 gaccagacaa gcgccgccca catgggtcgt ttgtccagtc ttaagtctca tcagcctcat
     6721 ccgttaccga atcagacatc ccaaccatgc gatgcgtcgc aaatgtgttg actgggattt
     6781 caagccaccg tgaaagcggc ctgtaatgat tactccctca agagttggcg cgattatagg
     6841 aaaaatcatt tgattaagca agtccatggc agggccacag ataacaaagc gacctgtaaa
     6901 tcatttagaa agtttacagg ccgctttaaa atcagcgttt atcgatggcg atttacttgt
     6961 cgatttgctt ttccacccga cgcagttggc ggaaccattt tggctcccaa gtacgcagtg
     7021 ccaacaccat cgccatgcca cgcttttggc tttgcggtac gggcagagat tgacggtgag
     7081 cttgcgccat ttgctgttgg cattttttca acaccgtgcg catcgcgtgt acgctgtcat
     7141 tgctgagcat gccgtggcca aacaaaattt cctcatgccc ttcttcatcg gagtgggcat
     7201 caaaaaaatc gcctatgcct tgttcttcga tgtagcgttg aatcgggcca ttgggctgcc
     7261 aattaaattg ctggctcacg cgcaaacgta cacggttatt cggcaacaat tgcaacatgc
     7321 ccattttgtc taactgggtc agcttcaaaa tgcactcagg caaatccact tggtaatgct
     7381 ctatcaccgc ttcaatcggc caatggttca tcatgcacac cgccaccaaa atcaattttg
     7441 ggtcttgcat caattgcttt tcttgcgcca gcgtcagttg gtcgagtaca ggccgctgct
     7501 catccaccaa acgcgccaat tcggcgaaat tcaattgcaa atactgacaa atttccaata
     7561 agcgctctaa ggtcacgctt tggttggcaa acaaacgctt gatgctggac tcagacacat
     7621 ccaagtgctt ggccaaatcg gcatacgtct tcccttgcgc atgcaattgc tgttttaaca
     7681 ccgcaaaaat ttgctgctgc tccatgtttt gctcccctta ttcatcccca gtagcgtcat
     7741 atgatacttt aaagccagta ctctgcgcaa aatttgcacg ttttacaccc ttcttgtgtg
     7801 gacatggcag catgtttcaa acacttagga gaaataccat gtctgcttca tcacctctca
     7861 acgctagtcc cagttgggcc ttgctcgcca ccatcacatt ggccttattg gcaacaggtc
     7921 acatcaacca attggcgcat taccatgatt ttgccgacca aacgcactgg ctaatgctgc
     7981 ccaatggcaa agacgtgtgg tcgaatatgg gtctgatact cataggctta tgggcatgtt
     8041 acggcttaaa acgtgcgcac atcccgttta caggccgcat cagtgcgtat gtgttggcca
     8101 gtgccattgt gttaacaggt ttgggctcaa gttattacca tttgcagcca aatgatttca
     8161 gcctgatttg ggacagattg cccatttcat tgatttgcgc aggactgctg ggcatggtgt
     8221 ttcaatacac ccatcaacgc agcgatggta tcgtgctcag tattgcaatc atcttcgcgg
     8281 tgcttggtgt gtggtattgg catgtcagtg gcgatttgcg cctgtacttg ggcatgcagg
     8341 tgttcaccat tttggctttg cccttgtggc tgtggcaagc gcgggcgcct cgcgccatca
     8401 ccttggcctt gttgtgggcc ataggctttt acatcgcctc caaagtcacc gaagcattgg
     8461 acgtacagct gtttgctctc agcagccata tcatcagcgg ccacactttg aagcacttgt
     8521 tcgcgaccat ggcaatggct tgcgtggtgg tggccatgcg cgccatggtg ctaacaccaa
     8581 tcagcgcaag cggcgtccgc tgtctaaatc gtaaatttgg ctaggttgct tgttgctacc
     8641 gatgcggcct gtaaccacca tcacttggcg accaaactga cgtttcactt cacgcgtggt
     8701 agtacaaaag cgtttgccac tgcgattgca tgaggtcgaa accaatggtg aggccgccaa
     8761 tttgctcaac aagcgggcat cgtcgtgagc aggcacacgc accgccaatt tgttgcggcc
     8821 attgccgcgc aataagggcg ataccttgtc ggaagagcga atgatgaagg ttttggcagc
     8881 gggccacgct tgggtgactt gctcggtttg cgcctcagtc agcggtttca
//
LOCUS       DAOMVK010000029         8917 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_2005_length_8917_cov_3.096367, whole genome shotgun sequence.
ACCESSION   DAOMVK010000029 DAOMVK010000000
VERSION     DAOMVK010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 8917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8917
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2005_length_8917_cov_3.096367"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(514..1806)
                     /locus_tag="PK856_01940"
     CDS             complement(514..1806)
                     /locus_tag="PK856_01940"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HRL01164.1"
                     /translation="MVKNVVVVGSQWGDEGKGKIVDWLTDQASGVVRYQGGHNAGHTL
                     VVGGKKTVLRLIPSGILHAGKRCFIGSGVVVSPEALLSEMAELESAGIDVAKQLTIAP
                     TCPLILPYHVALDHAREAAKGDKKIGTTGRGIGPAYEDKVARRAVRVMDLFQPELLRQ
                     KIEDVLAIYNCQLVGLYGQEAIQADAIYDLAMSLAEKIRPMVGDVSRLLNEINDKGES
                     LVFEGAQGTLLDIDHGTYPYVTSSNCVAGAASAGAGVGPHKLNYVLGIVKAYTTRVGS
                     GPFPTELFDDVGAGLAKRGNEFGAVTGRPRRCGWFDAALMKRSIQVNGISGMCVTKLD
                     VMDGMDEIKICVGYKLRGEIIDIFPFGADDVAACEPIYETMPGWTESTFGVKEYDALP
                     ANAKAYLDRLEAICGAKIAIISTGPDREETIVLEHPLQ"
     gene            complement(1827..2987)
                     /locus_tag="PK856_01945"
     CDS             complement(1827..2987)
                     /locus_tag="PK856_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HRL01165.1"
                     /translation="MQSWQLPEYIADILPSTARQLESAKEALLGLFRVHGYELVSPPL
                     LEYTSSLLLDIDEGLALKTVRIVDQISGRQLGVRADITPQVARIDAHLLAGNDGINRL
                     CYAGSTVHARPDSLLSTREPLQVGAELYGYEGIEADVEIIDLMLKTLTVAHAQDVYLS
                     LGHVGIFQALADKAALTQVQRDSLLYLMKDKDSEAVLAAMQDWDIDTIWKETFAVLPT
                     LYGDVETVLRRAREDLPAIKGVQDALDALKAVCKQFKTYKVHIDLSILRNDSYHTGLL
                     FAAYAPGWPDALARGGRYDGLGSQFGRARPATGFSLDLRDLIHVLPQRPARQGIKVAA
                     AVSNEAREEMNKLRHNGEVVVIDYLQDSAEALHCDRELVLKDGAWKIVPCDC"
     gene            complement(3118..3927)
                     /locus_tag="PK856_01950"
     CDS             complement(3118..3927)
                     /locus_tag="PK856_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRL01166.1"
                     /translation="MNFFEAIIIAIVEGLTEFLPVSSTAHMIFTSSFFGIQEDAFVKM
                     FMVSIQLGSILSVVVMYWRKFFDKNNLGLYVKLACAVLPALVLGKLFDDKIEAVLGSP
                     RPIAVMLIIGGVILLFVDKWFTKPVVFSEKEVTIRKAVAIGFWQCLAMMPGTSRSAAS
                     IIGGMQQGLSRQAAAEFSFFLAVPTMLAVTTYSLFVKEWGTEGQGLTGIEMMMATPQN
                     LPIFLVGIVVAFLVALVAMKFFIGIIKKYGFKPWGWYRIIAGIILFTYFSL"
     gene            complement(3999..4685)
                     /locus_tag="PK856_01955"
     CDS             complement(3999..4685)
                     /locus_tag="PK856_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003774026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HRL01167.1"
                     /translation="MLFKKLMAAALMTVGMAGVSQAALVEGTDFVVLPEAIPQLKQDK
                     IEVLEFFGYFCIHCKNLEPTMTAHVKTFPTDTYFRAEHVVWRPEHLGLARIAAAVNAT
                     GMKAQANPAIFKAVMDEGIDLSNPETFKQWAAQQSAFDGQKLLAAFNGFQNQTEAKNM
                     ADLSTKYKIQGTPTIIVGGKYQLQSQDMAKLDELIQKVRAERGMPAAQAKPVLKVKAT
                     GSRLAGQANQ"
     gene            complement(4708..5511)
                     /locus_tag="PK856_01960"
     CDS             complement(4708..5511)
                     /locus_tag="PK856_01960"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRL01168.1"
                     /translation="MAKKTSPRQSKSKSLGQTLNGILIGLILGAIIIAILLWFLNKQK
                     MPIEPPKASEPTKTEIMVPNQAELPHTDPTPTASETGTLGTENTGAASELVEHEESET
                     EKPVIVRPPQNKHETGSKAQTGEKKPQTTPPVDKKPEGKSTDNKTSSKPTPEQILEHG
                     SVEKARAANQKEAVKKQTEKAPAKEEAKASTGRSVKLQAGSFGSKEQAEAQRAKLALL
                     GISVQVETGQVNGKPVHRVRTARLNEDKAAQVRKQLSNNGIDSIAVGAN"
     gene            complement(5547..7073)
                     /locus_tag="PK856_01965"
     CDS             complement(5547..7073)
                     /locus_tag="PK856_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HRL01169.1"
                     /translation="MSLATVFSRALNGMDAPQVEVEAHLANGLPAFNIVGLPDTEVKE
                     SRDRVRAAILQSGFDFPSKKITVNLAPADLPKDSGRFDLPIAIGILVASGQVMADDLA
                     QYEFAGELALSGRLRPVRGALAMAISCAQSERSFVLPTESASQAALLERADIYAAESL
                     SEVAAHLNRISLLEPVLPTASVDDWVGLADLSEVKGQYSARRALEVAAAGGHNLLMMG
                     PPGTGKSMLAQRLPSILPPLTMDERLSVASIRSLVLQHSHEVLPKARPFQMPHHSASS
                     VALVGGGSDPRPGAISMAHFGVLFLDELPEFDRKVLEALREPLESGEIHISRASRNVV
                     FPARFQLVAAMNPCPCGYYGHASKPCRCSVETIQRYRDKISGPLMDRIDLIIDVPQLQ
                     EHDLTSDKRPESSNDVRERVLRAHERQMQRQGKSNSALSVDDLEQLALIATEAKLYLS
                     KLMNDLNLSARSYHRLLRVARTCADLHADDVVETHHIVEAIQFRRAWTNQLQLGQKPW
                     "
     gene            complement(7093..7419)
                     /locus_tag="PK856_01970"
     CDS             complement(7093..7419)
                     /locus_tag="PK856_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HRL01170.1"
                     /translation="MIAKKIFEEISSKLGDTIAQSPAKDLEKNVKSLMGSAFNRMDLV
                     TREEFDIQQQVLIKTRELLTQLEQRVAVLEAAQAFSSTTVEESVQTAEETAAKVVAEE
                     QQNKED"
     gene            complement(7487..7918)
                     /locus_tag="PK856_01975"
     CDS             complement(7487..7918)
                     /locus_tag="PK856_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01171.1"
                     /translation="MKKHVYLLIAIVAVAFVFAKVVAVLAFEDIKEGQALDAPMLPCQ
                     LQQGCRLGSETQVKSREELKLNQPFHLELTTTEAVPEVSVSFSMVEMEIGYNRYKLTS
                     EDGKHWQAEIRLPICTLNRADYIATWRVGEQKYQTALNVNR"
     gene            complement(7915..8670)
                     /locus_tag="PK856_01980"
     CDS             complement(7915..8670)
                     /locus_tag="PK856_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRL01172.1"
                     /translation="MTQKLVHVVAAVILNEQGEFLLSSRPEGKAYAGYWEFAGGKVEA
                     GETELQALQRELHEELGIHIHAATPWLTKIHHYEHASVLLRFFRVLPNQWHGEVMCRE
                     AQQYSWQQAGRFDVEPMLPANGPILRALSLPLSLHGNAHEGWLGVNGVGEVSMGFTNQ
                     DIRLLPINTNQTDYAGLQLQCLQAASEMQACTTDGWWWQVASTTEVDEVLAQLNQGCN
                     VPIVALLDEHHQHEAERLQAAGVHIAYTVSKQA"
     gene            complement(8667..>8917)
                     /gene="acpS"
                     /locus_tag="PK856_01985"
     CDS             complement(8667..>8917)
                     /gene="acpS"
                     /locus_tag="PK856_01985"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HRL01173.1"
                     /translation="QPVAFLAKRFAAKEAFSKAMNTGIRNPVTLGNIGVIHDEMGKPE
                     FWVAEPLQAWMDERGVKHVHLSLTDEGHYSVAFVVAEQ"
BASE COUNT         2383 a         2328 c         1816 g         2390 t
ORIGIN      
        1 tgttattcaa gtaacaattt gtattatttt tgtcagatta ccaacaaagt taaaagtggt
       61 aaagcaaatt tttagcatgt cacttaccac cccagacaca acttcaagtc atttttttgt
      121 taataatgga atataattta tgtttgcaaa ttatgtttgc tgaaaactat gaatccatat
      181 ttttagtttt gaaaatagac agatgaatta ccaataaaat tagctatcga ttcgcaacct
      241 tccttaaaaa tgcaattcaa atctgactga ggatattttt ataacataga aataactttc
      301 tctattttct cacctcaata taaccttacc aaatttactg tgattgtaaa ttcaacagtt
      361 agcgctcact ctctagccag cttcaggctt tcaaatgctt caagaaacat tgtagtcaat
      421 ataatagtga ggatatttct aactcagata ggtaaaaaaa gcggcctgta aattttacag
      481 gccgcttttt actgattggt tacattacaa atcttattgc aacgggtgtt ccaaaacgat
      541 ggtttcttca cggtctggac ccgttgaaat aatggcgatt ttcgcgccac agattgcttc
      601 caaacggtcc aaatacgctt tggcgttggc tggcaatgca tcgtactctt tcacaccgaa
      661 agtagactct gtccaacctg gcatggtttc gtagataggt tcgcaagcgg ccacatcgtc
      721 tgcaccaaat gggaagatgt caatgatttc gccacgcaat ttgtagccca cacagatctt
      781 gatttcatcc atgccatcca tcacgtccaa tttggtcacg cacatgccag agatgccgtt
      841 cacttgaatc gaacgtttca tcaaggctgc atcaaaccaa ccacaacgac gtgggcggcc
      901 tgtaacggca ccgaattcgt taccgcgttt ggccaagcct gcgccaacat catcaaacaa
      961 ttctgttggg aatgggcccg aacccactcg tgtggtgtaa gccttaacaa tgcccaacac
     1021 atagtttaat ttgtgtggac ccacacctgc gccagcagaa gcggcaccag ccacacagtt
     1081 agatgaagtc acgtatggat aagtaccatg gtcgatgtca agcaacgtgc cttgtgcacc
     1141 ttcaaacacc aatgattcac ctttatcgtt gatttcattc aacaagcgcg aaacatcgcc
     1201 taccataggg cggattttct cagccaaaga catggccaaa tcatagatgg catcggcttg
     1261 aatagcttct tggccataca atcccaccaa ttgacagttg taaatggcca aaacatcttc
     1321 gattttctga cgcaataact ctggttggaa caaatccatc acgcgcacag ctcgacgtgc
     1381 cactttgtct tcataagcag ggccaatacc acgacctgtg gtaccgattt ttttgtcgcc
     1441 tttggccgct tcacgcgcat ggtccaaagc cacgtggtaa ggtaaaatca atgggcaagt
     1501 tggggcaatg gtcaattgtt ttgccacatc aatgccagct gactctaact ctgccatttc
     1561 acttaacaag gcttcagggg aaaccaccac acctgagcca ataaaacagc gtttgcctgc
     1621 atgcaaaata ccacttggaa tcaagcgtaa aacggttttc ttaccgccta ccaccaaagt
     1681 atgacccgca ttgtgaccgc cttgataacg taccacgcca ctggcttggt cagttaacca
     1741 atcgacaatc ttgcctttac cttcgtcacc ccattgtgag ccgaccacga caacattctt
     1801 aaccattgtt tgttcctgca taaaaattaa caatcacaag gcacgatttt ccatgccccg
     1861 tcttttaata ccaattctct gtcacaatgc agtgcttcag cactgtcttg taaataatca
     1921 atcaccacca cttcaccatt gtggcgcaat ttattcatct cttcacgggc ttcatttgac
     1981 accgctgccg caaccttaat cccctgtctt gctggtcgtt gcggcaaaac atgtatcaaa
     2041 tcacgcaaat ccaagctgaa gccagtcgca gggcgcgcac gaccaaactg actgcccaag
     2101 ccatcataac gaccacctcg agccaaggca tcgggccatc ctggtgcata agctgcaaac
     2161 aataggccag tgtggtagga gtcgttgcgt aaaatcgata agtcaatgtg tactttgtat
     2221 gttttaaact gcttgcacac agctttcaaa gcatccaatg cgtcctgtac gcctttgatg
     2281 gcaggcaaat cctcacgcgc gcgacgcaat accgtttcca catcaccata taaggtgggc
     2341 aaaaccgcaa aggtttcttt ccaaatggta tcaatatccc aatcttgcat cgccgccaaa
     2401 accgcttcgc tgtctttgtc tttcatcaaa tacaacaaac tgtcgcgttg tacttgggtt
     2461 aaagcagctt tatcagccaa agcttggaag atacccacat gtcccaatga caaataaacg
     2521 tcttgagcat gggctactgt caatgttttc aacatcaaat cgatgatttc aacatccgcc
     2581 tctatgccct catagccgta taactcagcc ccgacttgca gcggttcacg tgtactgagc
     2641 aagctgtctg gacgtgcgtg taccgtagag ccagcgtagc aaagtcggtt aatgccatcg
     2701 ttcccagcca gtaagtgggc atcaatacgc gccacttggg gggtaatgtc tgcgcgcaca
     2761 cccagttgac ggccactgat ttgatcgacg atgcgcactg ttttcagtgc caaaccttcg
     2821 tctatgtcga gcaataatga ggaggtgtat tcgagtaaag gcggactcac aagctcatag
     2881 ccgtgtaccc taaaaagacc taaaagtgct tctttcgcac tttccaattg ccgtgcagta
     2941 gacggcaaaa tatcggcaat gtattcaggt aattgccaag attgcatgtg cgactcgctt
     3001 tgtttttaca aaagaaaagg cggggtttgc acccaccttc gacagtttta gtcctgatag
     3061 taacatatgc agcgaaaatc cgcagctaaa acctgtatca aacaggcatt attgcgatta
     3121 caaactgaaa tatgtgaaca agatgatacc tgcaatgatg cgataccaac cccatggttt
     3181 aaagccatat ttcttgatga tgccaatgaa aaatttcatc gccaccaagg ccaccaaaaa
     3241 tgccaccacg atgcctacca agaaaatggg caaattttgt ggggtagcca tcatcatctc
     3301 aatgcctgtc aaaccctgcc cttccgtgcc ccactcttta acgaataaag aataagtggt
     3361 cacagccaac atggtcggca cagccaagaa gaatgagaac tcagcagcgg cttgacgcga
     3421 taagccttgc tgcatgccac caataatcga tgccgctgag cggctggtgc ctggcatcat
     3481 tgccaaacat tgccaaaaac cgatggccac agccttacgg atagtgacct ctttctcaga
     3541 aaacaccaca ggcttggtaa accatttgtc aacaaacaat aagatgacac caccaatgat
     3601 gagcatcaca gcaatgggac gtgggctgcc taaaaccgcc tcgattttgt catcaaacag
     3661 ctttcccaac accaaagcag gaagaaccgc acaggccagt ttcacataca aacccaagtt
     3721 atttttatca aagaacttgc gccaatacat gacaaccacc gacagaatgc tgcccaattg
     3781 tatggacacc atgaacattt tgacaaatgc atcttcttgt atgccaaaga acgagctggt
     3841 gaagatcatg tgtgctgttg aagacacagg taaaaactca gtcaaacctt caacaatcgc
     3901 aatgatgata gcttcaaaaa aattcattat taattcaacc ttaaatgcgg cctgtaacaa
     3961 caaacaggca gctgtaagct gcctgttcta cgctaaaatt attgattggc ttggccggcc
     4021 aagcgtgagc ctgtggcttt gactttcaat actggcttgg cttgggctgc tggcataccg
     4081 cgctcagcac gcactttttg aatcaactca tccaatttgg ccatatcttg agactgcaat
     4141 tggtatttgc cacccacaat aatggtaggc gtgccttgaa ttttgtattt ggttgacaaa
     4201 tcggccatgt ttttggcttc ggtttggttt tgaaagccat tgaaagcagc caataatttc
     4261 tgaccatcaa acgcactttg ttgagctgcc cattgtttga aggtttctgg attgctcaaa
     4321 tcaatgcctt cgtccattac cgctttaaaa atagcgggat tggcctgagc cttcatgcct
     4381 gttgcattga ctgctgcggc aatgcgtgcc aaacccaaat gttcaggacg ccataccaca
     4441 tgctcagcac ggaaataggt atcggttggg aaggttttca catgagccgt catggttggc
     4501 tcaagatttt tacaatggat acagaaatac ccaaaaaact ccaacacttc aattttatct
     4561 tgcttcaatt gcgggatggc ttctggcaaa acaacaaaat ccgtgccttc taccaaagca
     4621 gcttgtgaca cgcctgccat acccactgtc atcaacgctg ctgccatcaa ttttttaaac
     4681 aacatacaca ttccttattt taaatgacta atttgcacca acggcgatgg agtctatgcc
     4741 attattgctt aactgtttgc gtacctgcgc agccttgtct tcattcaaac gggctgtgcg
     4801 tacacggtgg acgggtttac catttacttg gccagtttct acctgaacag aaatgcccaa
     4861 caaagccaat ttggcacgtt gtgcctcagc ttgctctttg ctgccaaacg agcccgcttg
     4921 caacttcact gagcggcctg tactggcctt tgcttcttct ttagcagggg ctttttcagt
     4981 ttgtttttta accgcttctt tttggttggc tgcgcgtgct ttttcaacac tgccatgctc
     5041 taaaatttgc tcaggtgttg gcttactgct ggttttattg tctgtggatt tgccctctgg
     5101 ttttttatcc acaggaggag tcgtttgtgg tttcttctca cccgtttggg ctttggatcc
     5161 cgtttcatgc ttgttttgcg gcggtctcac aatgactggc ttttctgttt cactttcttc
     5221 gtgttcaacc aattcacttg ctgcgccagt attttcagtg cctaaagtac ccgtttctga
     5281 tgcggtaggc gtaggatcgg tatgaggcaa ttcagcctga ttgggcacca tgatttcggt
     5341 tttggttggt tcagaagcct taggcggctc gataggcatt ttttgcttgt tcaaaaacca
     5401 caataaaatt gcaatgatga tggcacctaa aatcaaacca atcaaaatgc catttaaagt
     5461 ttgtcccaaa gatttggatt tgctttggcg cggactcgtt tttttagcca tgtaatgaaa
     5521 ctctctcagc gcttatgcgc ataatgttac caaggttttt gccccaactg tagttgattt
     5581 gtccaagccc gtcggaactg tatggcttca acaatgtgat gggtctccac cacgtcatct
     5641 gcatgtaaat ctgcacaagt acgtgccacg cgcagcaatc gatggtagct gcgggccgac
     5701 aaattcaaat cattcattaa tttggacaaa tacaattttg cttcggtagc aataagtgcc
     5761 aattgctcta agtcgtccac actcaatgct gaattgcttt taccttgacg ctgcatttgt
     5821 ctttcatggg ctcgcaacac ccgctcacgc acatcattgc tgctttcagg ccgcttatca
     5881 ctggttaaat cgtgttcttg caactgtggc acatcaataa tcaaatcaat gcgatccatt
     5941 aatgggccag atattttatc gcgataacgc tgtatcgttt ccacagagca ccgacaaggc
     6001 ttgcttgcat gcccataata accacagggg caagggttca tcgccgccac caattgaaat
     6061 cgcgcaggaa acaccacatt gcggctggca cgagaaatgt gtatttcccc actctccaat
     6121 ggctcacgca gcgcttccaa aaccttacga tcaaactcag gcagttcgtc caaaaacaac
     6181 acaccaaagt gagccatcga gatggctcct ggccttgggt cagaaccacc gcccaccaat
     6241 gccactgatg acgctgagtg gtgtggcatt tgaaacggcc tcgctttggg caacacctca
     6301 tggctgtgtt gtaaaaccaa agaacgtatg cttgccacgc tcaaacgttc atccatggtc
     6361 aatgggggca agatactggg cagtcgctgt gccaacatgg acttacctgt acctggaggc
     6421 cccatcatca acaaattgtg cccaccagcc gcagccactt ccaacgcacg gcgtgcgcta
     6481 tactgccctt taacctcact taaatctgct aagccaaccc aatcgtctac agatgctgtt
     6541 ggcagaactg gctccaacaa gctgattcgg ttcaaatgcg ccgccacctc actcagactt
     6601 tcggccgcat aaatatcagc tctctcaagc aaagccgctt gtgatgcact ctcagttggc
     6661 aacacaaatg atcgctcaga ttgtgcacac gatatcgcca tcgccaaagc accccgaaca
     6721 gggcgcaagc ggcctgaaag tgccaattca cccgcaaatt catattgcgc caaatcatca
     6781 gccattactt gacccgaagc aaccaatata ccgatggcga tgggcaaatc aaagcggcct
     6841 gaatctttgg gtaagtctgc aggtgccaaa ttaaccgtga tttttttaga aggaaaatca
     6901 aaacccgatt gcaatatggc cgctctaacc cgatcacggc tttctttgac ctcggtatcg
     6961 ggcaaaccca caatattaaa agcgggcaaa ccattggcaa gatgggcttc tacttccacc
     7021 tgaggtgcat ccatgccgtt caaagcccgc gaaaacaccg ttgccaaaga catggtttgc
     7081 tcctaaagag aatcagtcct ctttattttg ctgctcttct gccaccactt tggcagcagt
     7141 ttcttctgcg gtctgaaccg actcttcaac ggtagtgctt gaaaatgcct gtgccgcttc
     7201 caacaccgcc acacgttgct ccaactgggt cagcaactca cgggttttaa tcaaaacctg
     7261 ttgctggata tcaaactctt cacgggtcac caagtccatg cgattaaacg ccgaacccat
     7321 caaggatttg acattttttt ccaaatcttt ggctgggctt tgtgcaatgg tgtcaccgag
     7381 tttagacgaa atttcttcga atattttttt cgcaatcatt tgtgtgtcct tagttcaatt
     7441 gtgtttgatt cgctgccatt gtaatagaaa acgcttggct cgcgcatcaa cggtttacat
     7501 tcaaagccgt ctgatacttt tgttcaccca cccgccaagt agcaatgtaa tcggctcggt
     7561 ttaaagtaca aatgggcagt cgaatttcag cttgccaatg tttgccatcc tcactggtga
     7621 gcttgtagcg gttgtagccg atttccatct ccaccatgct aaaactcact gaaacctcag
     7681 gaaccgcttc agttgtggtc aactcaagat ggaatggctg attcaatttc aattcttcgc
     7741 gactctttac ctgagtttca ctgcccaagc gacacccctg ttgcaattgg catggcaaca
     7801 tcggcgcatc caatgcttgg ccctctttaa tgtcttcaaa ggccaaaacg gcaaccactt
     7861 tggcaaacac aaatgccact gccacgatgg caatgagcaa atacacatgt tttttcatgc
     7921 ctgcttgctc acagtatagg cgatgtgcac accagcggcc tgtaatcgct cagcctcatg
     7981 ctgatggtgc tcatcaagca aagccacaat tggaacatta cagccttgat ttagctgggc
     8041 caacacctca tcaacttcgg tggtacttgc aacttgccac caccatccat ccgtggtgca
     8101 agcctgcatt tcagaagcgg cctgtaagca ttgcaattgc aaacccgcat aatcggtttg
     8161 atttgtgtta attggcaata agcgaatgtc ttgatttgta aagcccatgc tcacttcgcc
     8221 gacgccattc acacccaacc atccctcatg tgcattgccg tgcaatgaca gtggtaaact
     8281 caacgccctt aaaatagggc cattggcagg caacattggc tctacatcaa atcgccctgc
     8341 ttgttgccat gagtattgtt gcgcctcacg gcacatcact tcaccgtgcc actgatttgg
     8401 caatacccga aaaaaccgca gcaacacact ggcatgctcg taatggtgga ttttggttag
     8461 ccatggcgtg gcagcatgaa tgtgtatgcc caattcttca tgcagttcac gttgcaaagc
     8521 ctgcaattcg gtttcacctg cttccacttt gccgccagca aactcccaat aaccagcata
     8581 agccttgcct tcggggcggg aactcagcaa aaattcgcct tgttcattca gtatcacagc
     8641 cgccaccaca tgtaccaatt tttgtgtcat tgctcagcca ccacaaaggc cacactgtaa
     8701 tggccttcat cagtcaaact taagtgcaca tgtttgacac cacgctcatc catccaagct
     8761 tgcaatggtt cggccaccca gaattcaggc ttacccattt catcgtgtat cacgccgatg
     8821 ttgcccaaag tcactggatt gcgtatgcca gtattcatgg ccttactgaa tgcttctttg
     8881 gctgcaaaac gcttggccaa aaaggccaca ggttgcg
//
LOCUS       DAOMVK010000030         8888 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_2015_length_8888_cov_3.405412, whole genome shotgun sequence.
ACCESSION   DAOMVK010000030 DAOMVK010000000
VERSION     DAOMVK010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 8888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8888
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2015_length_8888_cov_3.405412"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PK856_01990"
     CDS             complement(<1..423)
                     /locus_tag="PK856_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRL01174.1"
                     /translation="MSYPVRRFDAVIVGGGGAGLRAALQTSKAGLNTAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMTRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRT"
     gene            complement(420..770)
                     /gene="sdhD"
                     /locus_tag="PK856_01995"
     CDS             complement(420..770)
                     /gene="sdhD"
                     /locus_tag="PK856_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HRL01175.1"
                     /translation="MVDRKLTGAHYGLRDWAMQRITAVIMVIYTVAMIVILFTLPHEY
                     DAWRAFFTQGWVRMFTQITFIAVALHAWVGIRDLWMDYISKSAGLRLTLHVATIIWLV
                     ACVIYSFKVIWGLA"
     gene            complement(764..1138)
                     /gene="sdhC"
                     /locus_tag="PK856_02000"
     CDS             complement(764..1138)
                     /gene="sdhC"
                     /locus_tag="PK856_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HRL01176.1"
                     /translation="MHKQRPVYIDVVKTRLPIPGIVSILHRVSGVGLFICLPLLLCLF
                     AGTLSTEEAFNQYQSLVSNPIIKLLLLGLLWAYLHHFLAGIRFLFLDMHKGLELNTAR
                     TTAKVVFGAALILTVILGALLW"
     gene            complement(1274..2008)
                     /locus_tag="PK856_02005"
     CDS             complement(1274..2008)
                     /locus_tag="PK856_02005"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRL01177.1"
                     /translation="MPKSPTRNPLYAQVADYVMQQIAAGSYRPHQAIPTEWQLAKKLD
                     VSQGTVRKGLDELVFKQILYRQQGVGTFVADQYGDWGLYGLVGMFEKESHSFFPKSEV
                     LSVVAVHAGNEISDLLYLRAANPLVWKVLVLWRHQIHLVALDEIFLPYERLQDLNRRQ
                     LMTRLGIESLLLKEYELRLNDASCMVSVGGLSVDLANVLKVSAEEPVLYMQIKKTDAY
                     GEMMMLKRRYILTRHYQLNLSANSIV"
     gene            2226..3206
                     /locus_tag="PK856_02010"
     CDS             2226..3206
                     /locus_tag="PK856_02010"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HRL01178.1"
                     /translation="MKAPVRVAVTGAAGQIGYALLFRIASGEMLGKDQPVILQLLDLP
                     QAQNAVNGVMMELQDCAFPLLAGMVATDNPEVAFKDAQVAILVGARPRSKGMERADLL
                     AANAEIFTVQGAALNKVADRNVKVLVVGNPANTNAYIAMKSAPDLNPKNFTAMLRLDH
                     NRALSQVAEKTGKAVGDIQKLCVWGNHSPTMYADYRFATINGENVKDLINNQEWNANV
                     FLPTVGKRGAAIIEARGLSSAASAANAAIDHIHDWWLGTDGAWVTMGIPSDGSYGIPE
                     GTMFGFPVTCANGEYKIVEGLEIDAFSQERINITLAELEEEKAGVAHLLK"
     gene            complement(3258..3689)
                     /locus_tag="PK856_02015"
     CDS             complement(3258..3689)
                     /locus_tag="PK856_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01179.1"
                     /translation="MMSLRGIINFVLLAAFAYAAYCGYQYFQIRSAILEEIKGKPGVR
                     KMIGDIENVKIDLSQYPPVSCDEKKAVSWNDFVFKHHSKGCFRFSLGVQSDTLRTVKI
                     STDYDTNKLIGERFSYVMLCQKPGEIAAKDNGMPREYINCE"
     gene            complement(3686..4831)
                     /locus_tag="PK856_02020"
     CDS             complement(3686..4831)
                     /locus_tag="PK856_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HRL01180.1"
                     /translation="MAALKIFITCPRGLEEVLVGELNALALQHIHAVDGGVDCVGSWE
                     QVYQINLHSRVASRVLIELVRGVCAREDQMYQLAKKVVWTDWFTVDDSIKMHVTGKRT
                     PFKRIDILGLKIKDAVCDVFRDACGKRPSVDKMFPDVRIHAFVEGTQARIMLDTSGEA
                     LFKRGYRDETGEAPLRENLAAGLLMLAGYDGTQAFMDPMCGSGTIAIEAALMAKNQAP
                     GLYRQFGFEKLKGFKAEMWTKIKQQALSVMKEPVASLQAADVDRRVIKAAIANAHQAG
                     VDDVLAFKVQDMVNAKAGEAAGVMVSNPPYGIRLEDEHFLNGFYPQLGGVLKQQFHGW
                     QVGLISADMSLPKGLRLKPERKIPLFNGKLDCRLFIFKMVQGSNRGE"
     gene            4880..5953
                     /gene="mutY"
                     /locus_tag="PK856_02025"
     CDS             4880..5953
                     /gene="mutY"
                     /locus_tag="PK856_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HRL01181.1"
                     /translation="MSSVSFAHNFPLSHFSHALIAWQQQSGRHNLPWQQTDPYLVWVS
                     EIMLQQTQVSTVLNYYPRFITRFPTVQSLAEASIDDVTALWAGLGYYSRARNLHHAAQ
                     QVCEEFGGTFPHTRTDLEHLKGVGRSTAAAIAAFAFKQRETILDGNVKRVLCRLFALY
                     GNPQDKAFENSLWELAEQLLPEQAQDMPSYTQGLMDLGATVCTRSKPKCEACPMQSMC
                     RAHEQDLTAELPVKKTSVQVKQMPVYWLCVIDEKQRILLAKRPVKGIWSQLYCPPMIN
                     VDEWTNWLLEHDIADEGLVYLPPIAHKLTHRAMNITAAILYSNEDLGMVKNGQFYDLQ
                     AALQLGLPKPLQNLLPHLLTELP"
     gene            6092..8305
                     /locus_tag="PK856_02030"
     CDS             6092..8305
                     /locus_tag="PK856_02030"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HRL01182.1"
                     /translation="MVSLTRVNSPEQTLTPQQWLEHFSSKFAGEDLVLLQGALSLAQE
                     YYPEDAKTQMGEPMLSHVLSATYFVDELHIFADALAATVLSALPVYCKDWQALTTEKC
                     NANITRMVEGIDQVQKLTKFASIEKINTPEEHQQQAESMRKMLIAMVSDIRVVLIKLA
                     MRTRTMHFMASIPDSDLKREIAKETLDIFAPLANRLGVWQLKWLLEDLGFRYQNPEAY
                     SRIAKLLDEKRTERLEYINNVVGVIERELGRLDIHCEVAGRPKHIYSIYKKMTKKKLD
                     FSGLYDIRAVRVLVDIIPQCYTTLGIIHSMWQPIPGEFDDYIAHPKSNDYQSLHTVVV
                     GPKDKGVEIQIRTFDMHQFAEFGVAAHWRYKEGGEGDKAYEQKISWLRQLLEWRENVA
                     DNEQDSNLAEAFQTELFNDTIYVLTPHGKVLSLPVGATPIDFAYAVHTDLGHRCRGAK
                     VNGQIVPLSTALENGQRVEIITVKEGHPSVNWLLDGWVKSSKATSKIRAFIRAQNAES
                     VRENGRTLLDKQLNKLSIRPNLQQLCETLGYTKLDDLYLALGHGEVTPRNISNAANQL
                     MRPEEATTEFTEANIVRKSRAQTDKSGVLVDGEGGLLTFLAKCCKPAPPDAIAGFVTK
                     GRGISVHRQNCAAYQNLAQLHSDKIVSTQWSELKSGQVFPIDIEITARDRNGLLRDVS
                     EAFTRNKLNVIGVNTITKDALATMRFTVEIKQVEELPRVMSNISDVKGVYTVNRL"
     gene            complement(8603..>8888)
                     /locus_tag="PK856_02035"
     CDS             complement(8603..>8888)
                     /locus_tag="PK856_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRL01183.1"
                     /translation="AENVLNKAEALAKSLGATQIQRHITVSVKGARDMAQQLVDYAAQ
                     NSADLLVLGTHGRTGLMHLLMGSFAETVMRQTTLPLLIIRGTDEDADDEE"
BASE COUNT         2550 a         2271 c         1816 g         2251 t
ORIGIN      
        1 ggtacggtcg gccactgcac aagcacgctc taccgcgcgt ttaccgtgtt cggcagtgtg
       61 gccaccgaat ggacgttggt agattttgcc gctttctacg cggtcaaaag gcatacccat
      121 gtgttccaat tcgataacgg cttcaggtgc tgcacgggtc atgaattcga tggcatcttg
      181 gtcgcccaac cagtccgaac ctttaacggt atcgtacatg tgccaatccc aacggtcttc
      241 ttgcacattg cccaaagaag cagaaatgcc cccttgtgcg gcaactgtgt gtgagcgagt
      301 tgggaatact ttagacaaaa ccgctgtgtt caggccggct ttagaagtct gcaaggctgc
      361 acgtaaacct gcaccaccgc caccaacaat aacggcatca aaacgacgaa caggataact
      421 catgccaacc cccaaataac tttaaatgaa taaataacgc aagcaaccaa ccaaataatg
      481 gttgccacat gcaaggtcaa acgcaatcca gcagatttac tgatgtaatc catccacaag
      541 tcacgaatac ctacccatgc atgcaatgcc acagcaatga aagtgatttg cgtgaacatg
      601 cgcacccaac cttgggtaaa gaaagcacgc catgcatcgt attcatgcgg caaagtgaac
      661 aaaataacaa tcatagcaac agtgtaaatc accatgatga cagctgtgat gcgttgcatt
      721 gcccaatcac gtaaaccgta atgtgctcct gttaatttac gatctaccat aacaaggctc
      781 ctaaaatcac agtcaaaatc aaagccgcac caaacacaac tttagcggtg gtgcgagcgg
      841 tattcaattc caaaccttta tgcatatcca agaacaaaaa gcggatacca gccaaaaaat
      901 ggtgcaagta agcccacaac aaacccaaaa gtaacaattt aatgattgga ttgcttacca
      961 agctttggta ttggttaaaa gcttcttctg tgctcagcgt gcctgcaaac aaacacagca
     1021 ataacggcaa gcagatgaat aagcctacac cactgaccct gtgtaaaatg gacacaattc
     1081 caggaattgg cagacgcgtc ttcacaacgt ctatatagac aggtctttgt ttatgcattg
     1141 aggggttcct taaaaaaact gcgtaagtta ctaaataaac aactttttaa ttttaatacc
     1201 aatgaataca ttaatttacg ctagcgcaat ttactctttt ttaaacacca tgaaaagtat
     1261 tcattaccag aatttataca atggagttgg cagataaatt caattgataa tggcgtgtca
     1321 gtatgtatct tcgtttcaac atcatcattt caccataggc atcggtcttt tttatctgca
     1381 tatacagcac aggttcttct gcagagactt tcaaaacatt ggccaaatca acagacaatc
     1441 cacccacact caccatacaa cttgcatcgt ttagcctaag ctcatattct tttaataaca
     1501 gactttctat tccaagtcgc gtcattaact ggcgccgatt caaatcttgc aaacgctcat
     1561 aaggcagaaa aatctcatcc aacgccacca aatgaatttg atgccgccac aaaaccaaca
     1621 ccttccagac caatgggttc gctgcgcgca gatacaataa atcactgatt tcattacccg
     1681 catgtaccgc caccacgctt aacacttcac tcttagggaa aaaactgtgc gactcttttt
     1741 caaacattcc caccaagccg taaagtcccc aatcgccata ttggtctgct acaaaagtcc
     1801 ctaccccttg ttgacgatac aagatttgct tgaataccaa ttcatccaaa cctttacgca
     1861 cagtaccttg actgacatct agctttttag ccaattgcca ctctgtcggt atggcttgat
     1921 gcggcctgta acttccagca gcaatttgtt gcatgacata atcagcaact tgagcataca
     1981 aggggttacg tgtaggtgat tttggcatgc gtgtattgta acgcctgaaa ttaaaaagta
     2041 aattatgtct tatataagat ataagatatt caactaaata tattaataaa atcttttacg
     2101 agagtcatca agttttgcta tgattcatgt cggctcgaac cgccctgcca tgtaaattta
     2161 tgtgactgta aagacggtat cgatactaaa ttgtcgtaac tcgttattca tacgtggaga
     2221 taaaaatgaa agcacctgta cgtgttgctg taaccggcgc agctggtcaa attggctatg
     2281 ccttattgtt ccgtatcgcc agtggtgaaa tgttgggcaa agaccaacct gtgattttac
     2341 aattattgga tttaccacag gcccaaaatg ccgttaatgg cgtgatgatg gaactgcaag
     2401 actgtgcatt cccattattg gctggtatgg ttgcaaccga caaccctgaa gtggctttta
     2461 aagatgcgca agtggccatt ttggttggtg ctcgccctcg ctctaaaggc atggaacgtg
     2521 cagacttatt ggctgctaat gctgaaatct ttactgttca aggtgcagca ctgaataaag
     2581 tagcagaccg caatgtaaaa gtattggtag taggtaaccc agccaataca aatgcttaca
     2641 tcgctatgaa atcagctcct gatttgaacc cgaaaaactt cactgccatg ctgcgcttag
     2701 atcacaaccg tgcattaagc caggtggctg aaaaaactgg caaagcagtt ggtgacattc
     2761 aaaaattgtg tgtttggggc aatcacagcc ctaccatgta tgccgactac cgttttgcta
     2821 ccatcaatgg tgaaaacgtt aaagacttga tcaacaatca agaatggaat gctaacgtgt
     2881 tcctacctac cgtgggcaaa cgtggtgcgg ccatcattga agcccgtggc ttgtcttctg
     2941 ccgcttctgc tgccaacgca gccatcgatc acatccacga ctggtggttg ggcactgatg
     3001 gcgcttgggt aaccatgggt attccttctg atggctctta tggcattcca gaaggcacta
     3061 tgttcggctt cccagtgact tgtgctaatg gcgaatacaa aatcgttgaa ggtttggaaa
     3121 tcgatgcttt cagccaagaa cgcatcaaca tcacattggc tgaattggaa gaagaaaaag
     3181 cgggcgtagc tcacttgttg aaataacctc ttaagcttca caaaaaaagc cgcttaatta
     3241 agcggctttt tatttaatca ctcgcaattg atatactcac gcggcatacc attatctttc
     3301 gcagcaatct ctcctggttt ttggcacaac atcacatatg aaaaacgctc acctatcaat
     3361 ttattggtat cataatcagt agagattttc actgttctca aagtatcact ttgcactccc
     3421 aatgaaaatc taaaacatcc tttactgtga tgtttaaaaa caaaatcatt ccagctgact
     3481 gcttttttct catcacaact aactggtgga tattgactca aatcaatttt aacattttct
     3541 atgtcaccaa tcattttcct cacaccaggc ttgcctttga tttcctccaa aatagcagag
     3601 cgaatttgaa aatattgata gccacaatag gcggcatacg caaacgcagc cagcagcaca
     3661 aaattaatta tcccccgtaa actcatcatt ctcccctatt tgatccctgt accattttaa
     3721 atataaacaa acggcaatcc aatttgccat tgaacaatgg aattttgcgc tcaggcttta
     3781 aacgcaaccc ttttggcaaa ctcatgtccg cactgattaa acccacttgc cagccatgaa
     3841 actgctgttt caagacccct cccagttgtg ggtaaaaccc atttaagaaa tgctcatctt
     3901 ccaaccgtat tccataaggt ggatttgaga ccatcacgcc agcagcctcc cctgcttttg
     3961 cattgaccat atcctgaact ttaaatgcca atacatcatc cacacctgct tgatgcgcat
     4021 tggcaatcgc ggctttaatc acccttctat ccacatcagc ggcctgtaag ctcgccacgg
     4081 gctctttcat cactgacaaa gcttgctgct tgattttggt ccacatctca gctttgaacc
     4141 cttttaactt ttcaaaacca aactggcggt ataaaccagg tgcttgattt tttgccatca
     4201 acgccgcttc aatggcaatc gtgccactgc cacacattgg gtccataaat gcctgagtgc
     4261 catcgtatcc agctaacatc aacaagcctg ctgccaaatt ttcacgcaat ggcgcttcac
     4321 ctgtttcatc gcggtagccg cgcttaaaca aagcctctcc actggtgtct agcataatcc
     4381 gtgcctgcgt gccttctaca aatgcatgaa tgcgtacatc aggaaacatt ttgtcaacac
     4441 tggggcgctt gccacacgca tcacgaaaca catcacacac cgcatctttg attttcaaac
     4501 ccaaaatatc gatgcgttta aatggcgtgc gcttgcctgt cacatgcatt ttgatgctat
     4561 cgtcaacggt aaaccaatcg gtccacacca cttttttggc cagttggtac atctgatctt
     4621 cgcgcgcgca cacgccacgc actagctcta tcaacacccg cgaggccaca cgagaatgca
     4681 agttaatttg atagacttgt tcccaacttc ccacgcaatc cacaccacca tcaacagcat
     4741 gtatgtgttg caatgccaag gcattcaact caccaaccaa aacctcttcc aaaccacgtg
     4801 ggcaagtaat aaaaatttta agtgcagcca taaccaaact ttgtgacaaa atagcgcttt
     4861 attgtaactg aatcacgtca tgtcatcggt atcatttgcg cataattttc cactttcgca
     4921 cttttctcac gcattaatcg catggcagca acaatcaggc cgccacaacc tgccttggca
     4981 acaaaccgac ccttatttgg tgtgggtatc tgaaatcatg ttgcaacaaa cccaagtcag
     5041 cactgtgctg aattattatc ctcgcttcat cacccgcttc ccaaccgtac agtccttggc
     5101 agaggcttca atcgacgatg tcacagcgct ttgggcgggc ttgggctatt acagccgcgc
     5161 tcgcaatttg caccatgctg cccaacaagt ttgtgaagaa ttcggaggaa catttccgca
     5221 cacacgcacc gatttagaac atttaaaagg cgttggccga agcacggctg ccgccattgc
     5281 ggcttttgcc ttcaagcaac gcgaaaccat tttagatggc aatgtaaaac gggtgttgtg
     5341 ccgattgttt gcactgtatg gcaatccaca agacaaggcg tttgaaaaca gtttatggga
     5401 gctggccgaa caattgctgc ctgaacaggc acaagacatg cccagttaca ctcaaggctt
     5461 aatggatttg ggcgcgacag tttgcacccg tagcaaaccc aaatgcgagg cttgccccat
     5521 gcaatccatg tgtagggctc acgagcagga tttaaccgcc gaactgcctg ttaaaaaaac
     5581 cagcgtacag gtgaaacaaa tgcctgtgta ttggttgtgc gtgattgatg agaagcagcg
     5641 tattttatta gccaagcggc ctgtaaaagg catttggagc cagttgtatt gtccacctat
     5701 gatcaatgtg gatgaatgga caaactggct gcttgagcat gacattgcag acgaaggttt
     5761 ggtctatttg ccgcccattg cgcataaact cacccaccgc gccatgaaca ttacagcagc
     5821 catcttgtac agcaacgagg acttgggtat ggttaagaac gggcagtttt atgatttaca
     5881 ggccgccttg caattgggct tacccaaacc cttacaaaac cttttgccac acctgctgac
     5941 cgagttacct taaagcaatg tttttttatc ctcataatct gacatctgaa ctcggttaaa
     6001 ccgataatta actatgccaa aatcatataa agtttgcggt ataatgtaaa taatccttcc
     6061 gaatacagca tttccagcac atggagcaga gatggtatcc cttaccagag tcaattcacc
     6121 tgagcaaaca ctgacgccac aacagtggct ggagcacttc agtagcaagt ttgctggtga
     6181 agacttggtt ctgttgcaag gtgctttgtc tttggcgcaa gaatattacc cagaagacgc
     6241 caaaacacag atgggtgagc ccatgctctc gcatgtgtta agcgccacct attttgtcga
     6301 tgaattgcat atttttgccg atgccttggc tgcgacggtt ttatcagccc tgcctgttta
     6361 ctgcaaagac tggcaagcac tcaccactga aaaatgcaat gccaacatca ctcgtatggt
     6421 agagggcata gaccaagtcc aaaaactgac caaattcgcc agcatcgaaa aaatcaacac
     6481 cccagaagag caccaacagc aagccgaatc catgcgcaaa atgttgattg ccatggtgtc
     6541 agacatacgt gtggtgttga ttaaattggc aatgcgtacg cgcaccatgc acttcatggc
     6601 cagcatccca gacagcgatt taaaacgtga gatagccaaa gaaaccttag acatttttgc
     6661 gccactggcc aaccgcttgg gtgtgtggca attgaaatgg ttgctcgaag acttgggctt
     6721 tcgctaccaa aatcccgaag cctatagccg catcgccaaa cttttggatg agaaacgcac
     6781 cgagcgcttg gaatacatca acaatgtggt gggtgtcatt gaacgcgaat tgggtcgtct
     6841 cgacattcat tgtgaagtcg caggcagacc gaaacacatt tactctatct acaaaaaaat
     6901 gaccaagaaa aaactcgatt tttctggcct gtacgacatt cgtgccgtgc gcgtgttggt
     6961 ggacatcatt cctcaatgtt ataccacctt gggcattatc cacagcatgt ggcaaccgat
     7021 acctggtgag tttgacgatt acatcgccca ccctaagtct aatgattacc aaagcctgca
     7081 caccgtggtg gttggcccca aagacaaagg ggtggaaatc caaattcgca cgtttgacat
     7141 gcaccaattt gccgaatttg gcgtagcagc gcactggcgc tacaaagaag gcggagaagg
     7201 cgacaaggct tacgaacaaa aaatttcgtg gctcagacaa ttattggaat ggcgcgagaa
     7261 tgttgccgac aatgaacaag acagtaatct ggctgaagcc ttccaaaccg aattgttcaa
     7321 cgacaccatt tatgtgttaa cccctcacgg taaggtgttg tctttgcctg tgggcgccac
     7381 acccatagac ttcgcctacg cggtacacac agatttgggc caccgctgtc gcggcgccaa
     7441 ggtcaatggt caaatcgtgc ctttgtccac tgctttggaa aacggccaac gcgtagaaat
     7501 catcaccgtc aaagaaggcc acccttcagt caactggttg cttgatggct gggtaaaatc
     7561 ctccaaagcc accagtaaaa tccgtgcctt tattcgcgcc cagaatgccg aatcggtacg
     7621 cgaaaatggc cgcactttgt tggacaaaca attaaacaaa ttatcgatac gccccaactt
     7681 gcaacaactg tgtgaaacat tgggctacac caaactcgac gatttgtatt tggctttggg
     7741 tcatggcgaa gtcacgccgc gcaacatcag caatgcagcc aaccaattga tgcggcctga
     7801 agaagccacc accgaattca ccgaagccaa tattgtgcgc aaaagccgcg cccaaaccga
     7861 taaaagcggt gtgttggtcg atggtgaagg tggcttactc accttcttgg ccaaatgctg
     7921 taaacccgca ccgcccgatg cgattgcagg ctttgtcacc aagggacgcg gcatttcggt
     7981 acaccgtcaa aattgcgccg cctaccaaaa cttggcgcaa ttacactctg acaaaatcgt
     8041 ttccacccag tggagcgaac tcaaatcagg gcaagtcttc cccatcgaca tcgaaatcac
     8101 cgcgcgcgac cgcaatggct tgctgcgcga tgtctccgaa gcctttaccc gcaacaagct
     8161 caatgtgata ggtgtgaata ccatcaccaa agatgccttg gccaccatgc gtttcactgt
     8221 ggaaatcaaa caagtggagg aattgccacg ggtaatgagc aacatcagtg atgtcaaagg
     8281 cgtgtacacg gtcaaccgtt tgtaagcagc tcttgcatat cactagtagc ggcctgtaag
     8341 tcccaagcat ttacaggccg cttctgtgct gattcactgc ggaatgctac acctgttaat
     8401 ccacatcttc atactgatgc ttctgcatcc ttgctgctca cagattggta actgtggaaa
     8461 cctaaacaca aatccaacct ttacatttat ccaacaatga aatgcttggg acaactcaat
     8521 taggcagcac tccctacccc aaacaatcca aataaaagcg gcctgtaaca ttacaggccg
     8581 ctttacttat ttaaaatcag acttattctt cgtcgtcagc atcttcatcc gtgccacgaa
     8641 tgatgagcaa tggcaaagtg gtttgacgca tcacagtttc ggcaaagctg cccatcaaca
     8701 agtgcatcaa gcctgtgcga ccgtgcgtgc ccaacaccaa caaatccgcg ctgttttgcg
     8761 cggcataatc caccaattgt tgcgccatgt cgcgcgcacc ttttaccgat acggtgatgt
     8821 ggcgttggat ttgggtcgcg cccaaagact tggccaaagc ctcagccttg ttcaacacat
     8881 tctcagca
//
LOCUS       DAOMVK010000031         8837 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_2028_length_8837_cov_3.485994, whole genome shotgun sequence.
ACCESSION   DAOMVK010000031 DAOMVK010000000
VERSION     DAOMVK010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 8837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8837
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2028_length_8837_cov_3.485994"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="PK856_02040"
     CDS             complement(<1..584)
                     /locus_tag="PK856_02040"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRL01184.1"
                     /translation="MSKYLQIAVSAALISVSSLSYAQDAAYAVPDGYSAKVVEDIFKK
                     GGNAVDAAVATAFTLAVTYPEAGNIGGGGFMTVYMNGKPYFIDYREVAPKAATKDMYL
                     DEKGEVIENMSVVGAKAAGVPGTVMGLWEAHKKFGKLKWAEVVQPAIGYAENGFEVAD
                     TQSLYRTDVEKMYDGKTNFSKYFGDLKTGVNFKQP"
     gene            complement(701..1324)
                     /locus_tag="PK856_02045"
     CDS             complement(701..1324)
                     /locus_tag="PK856_02045"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009118390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HRL01185.1"
                     /translation="MMNQSATVLTINQTRAAEQAAVDAGSSFWQLMETAGTAAALDLL
                     KRPVNAQSYWILCGKGNNGGDGLVMARVLAQAGKHVTVSLMQGEVSSDLASQNLAALP
                     DNVLVQAPAVALLLNHDCVIDAVFGIGFEGELSEDLQAVFHALASRPCLRVALDIPSG
                     IHADCGHIAAHSFQADVCYAFHSLKPAHVMPSVQTHCGDIKVLDLGM"
     gene            1441..1953
                     /locus_tag="PK856_02050"
     CDS             1441..1953
                     /locus_tag="PK856_02050"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRL01186.1"
                     /translation="MKFKFRSMAAVLALSLPNAAWAWQQECEPQFDSYAQVLKEANKG
                     NAEAQYWLGEAYFDGESQFEQPSAVDYTQARAWYDKAVAQKDACAQHAIGWMYDSGTG
                     AEEDKAQAFAWYEKAAKQGLSKSQFNLGVLYRDGLGVAQNMKSAKSWFEKAAAQGDEE
                     AVAELADWSE"
     gene            complement(2055..3500)
                     /locus_tag="PK856_02055"
     CDS             complement(2055..3500)
                     /locus_tag="PK856_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01187.1"
                     /translation="MINLYEILKIEPTASEKSIQAGIAIYRLREDADEKIVAAARQWL
                     LNPAVRDQYNRKLFAEIGMPNTAPTAEPVATAAPQYHVDSKLIPTTQLNDYEHLYQFL
                     GVRTKATYAELADAIDEAVLEGLNGKTIQLARETLLDPDSRAAYDAEHGIESAAAIMD
                     AAEPVSRLPHTGLRLPAWLSFGKRKAKPSTTSVPVTPTEETEPTTSHQTNELDIPHSA
                     QPFDDDTGVKNDIQAAQKATPAPTIAPKPQFKRDNWYHFLGIRGDASFQEIQVAIRNA
                     RLKGRDKELIRQAQNMLQKPGNRRLYDKQNNIAAYIAAQNGDVTGHVKATDYRPAPQL
                     LGMSLQKILLAVFALIGLASALMPWAQNVLGLRFGLAWPLFGLSILTLACVASGDFRD
                     PVYRPASRILIVAMLPVAILMYMLGARQFQYGVYWGMAAMIGIAVLSELLGQQSKNVS
                     YRAKGQQLFAILALVLNLIALLMVWWFVRSH"
     gene            3704..4642
                     /locus_tag="PK856_02060"
     CDS             3704..4642
                     /locus_tag="PK856_02060"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRL01188.1"
                     /translation="MKKQIAMALMLSAMTYGASAAEFMKPNTEINNAGKHVVINIPQL
                     RLFVYENGKLAKSWPIAVGKGRTQTPPGEYDIGVKAFNPTWHIPASIQKERASKGLPA
                     LKTIPPGPKNPLGPVFVRFGDPKLGLGIHGTSAPASVPSFASHGCVRLRSENALEFAK
                     FIDKGSRVSVIYNESALNLDANNNLWVSAYKDPYNLKKMNPAAVKAQAQTIAQKYGKT
                     VNEKMLTQVLANRSGQLNCLTCAKGSAKVQGELRPVSMTFKTPPPPPVTANPTPLPSS
                     APITEPGDNGDDYIKPHKPVDWSSQLEVRDSFARLW"
     gene            complement(4737..5294)
                     /locus_tag="PK856_02065"
     CDS             complement(4737..5294)
                     /locus_tag="PK856_02065"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HRL01189.1"
                     /translation="MTTRNREETQSILDTADLLFSEAQCDEALERMAAEITRDLGDTY
                     PLVLPVMGGAVVFTGKLLPLLRFPLDFDYVHVSRYGDKLEGGNFSWTRAPQDKNIAGR
                     DVLILDDILDEGQTMRVIKEQVMAMGAKSCKTAVFANKLLDHEKPITADYVGLDVPNR
                     YVFGYGMDVSGAWRNLSAIYALKGH"
     gene            complement(5662..6123)
                     /gene="dtd"
                     /locus_tag="PK856_02070"
     CDS             complement(5662..6123)
                     /gene="dtd"
                     /locus_tag="PK856_02070"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HRL01190.1"
                     /translation="MRAVVQRVAHAQVDVLSANSKHTSGEIQQGLMVLLGVAHGDTDS
                     DARYIADKIAHLRVFTDAEDKMNLSVLEVGGSVLLVSQFTLYGDARNGRRPSYIEAAR
                     PEKAAHVYELVAKLLRAHGLIVATGEFQTHMQVSLCNDGPVTLLLDSDKQF"
     gene            complement(6230..6433)
                     /locus_tag="PK856_02075"
     CDS             complement(6230..6433)
                     /locus_tag="PK856_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003791841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HRL01191.1"
                     /translation="MKTMVAKFALIGVLGASLMGCANLDRQQRNTAAGAVIGGAAGHL
                     IGGNTGSTLGGAALGGVIGSQVR"
     gene            complement(6795..7247)
                     /locus_tag="PK856_02080"
     CDS             complement(6795..7247)
                     /locus_tag="PK856_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRL01192.1"
                     /translation="MSFGSINDGGNDTPMAEINVTPLVDVMLVLLIVFMVTMPVLTHS
                     IPLNLPTAAEKQQESQKEQVKEPLRLSITADGKYYIGANKEEEITPEALSQRLKAALA
                     KDPETVLAIAADKVSEHEALTKVLSAAQEAGLTKVGFVTETPAVLPAK"
     gene            complement(7257..7919)
                     /locus_tag="PK856_02085"
     CDS             complement(7257..7919)
                     /locus_tag="PK856_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRL01193.1"
                     /translation="MNLGLVFQQGDGVLIFVFLLLVAMSIATWSIIIWRSYRLIKAKK
                     ANRIEAEKFWEASSVEDAQRAANPQYSPLAQMITESIECKNTFQERSSKGNLSAQISF
                     DAYMVRHIRNAMTKALRPFESGLTTLASIGATAPFIGLFGTGWGIYHALINISNSGQM
                     SIAAVAGPIGEALVATAAGLFVAIPAVLAYNALVRGNKTLSNELDSFAHDLHAQLADK
                     NH"
     gene            complement(7990..>8837)
                     /locus_tag="PK856_02090"
     CDS             complement(7990..>8837)
                     /locus_tag="PK856_02090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRL01194.1"
                     /translation="HSQLQLATMKKNTHFALTITAVSLLHIAGLWAMSNFDDKPAELA
                     APQEELTFVELGGGAPAPAPAPLPVAQAKPEPKPEPKRERKPEPDVKRTIVKKPQEKT
                     VVERKPEPKPEQKPERKPEPERKPEQKTEKSNQRQQSANNQSSAQQSDNKGNAAQGTG
                     TNANNNGKGGDNNKGGNDGKGSGGGSGPSSNVKIAGSANCLSPEYPQEALDRGETGVV
                     RVRWVVGADGRINSVDVVGSSGSSRLDKAAKRKAATCKFKPAVKNGVPVQDSFAASYR
                     FNIKQ"
BASE COUNT         2155 a         2151 c         2280 g         2251 t
ORIGIN      
        1 ggttgtttga aattcacgcc tgttttcaaa tcgccaaaat acttggagaa attggttttg
       61 ccgtcgtaca ttttttccac atcggtgcgg taaagcgatt gtgtgtctgc cacctcaaag
      121 ccgttttcag catagccgat ggcaggctgc accacttcgg cccatttgag tttgccaaat
      181 tttttgtgcg cttcccacaa gcccatcacc gtgccaggca cacctgcggc tttggcgccg
      241 accacgctca tgttttcgat gacttcgcct ttttcatcca aatacatgtc tttggtggcg
      301 gctttgggtg cgacttcgcg gtagtcgatg aagtagggtt tgccattcat gtacaccgtc
      361 atgaagccac cgccaccgat attgcctgct tcagggtagg tcaccgccaa ggtaaatgcg
      421 gtggccacgg ccgcatccac ggcattgccg ccttttttga agatgtcttc caccactttg
      481 gcgctgtagc catctggcac ggcataagcg gcatcttggg cataggataa ggagctgacc
      541 gaaatcagcg cggcagacac cgcaatttgc aagtatttgg acatgtgtag cgcttcctct
      601 atttgttctt tgattatgac gatggcgcaa aaccatcagt ggagcatcat aaagaaatcg
      661 cttgaaattg cctagctgct tgaatgtgca cctgcaaaca tcacatgcct aggtccaaca
      721 ccttgatgtc gccacaatgc gtttgtacag aaggcatcac atgggcgggc tttaaactgt
      781 gaaacgcata acacacatcg gcttgaaaag aatgggcggc gatgtggcca cagtcggcat
      841 ggatgccact ggggatgtcc aaggccacgc gcaggcaagg gcgcgatgcc agcgcatgaa
      901 acacggcctg taaatcctcg ctcaattcgc cttcaaaacc gatgccgaaa accgcatcaa
      961 tcacgcaatc atgattgagc aacaatgcca ccgctggcgc ttgtaccagc acattgtctg
     1021 gcaatgccgc caaattttgg ctggccaagt cgctgctcac ttcgccttgc atcaagctca
     1081 cggtgacgtg tttgcctgct tgcgccaata cccgcgccat caccaagcca tcgccaccat
     1141 tattgccttt gccacacaaa atccaataac tttgtgcgtt tacaggccgc ttgagtaagt
     1201 ctagtgctgc ggctgtgcct gcggtttcca tcaattgcca aaacgagctg cccgcatcga
     1261 cggcagcctg ttccgctgca cgggtttggt taatggtaag tacggtagca gattggttca
     1321 tcataagcgg cctgtaaaaa agaatataaa tctgtttgat tttatgggga attcagtgct
     1381 gcgattttga cgcagatgtc gctatactcg cgtattcaca tctatcataa gggaatggtc
     1441 atgaaattca aatttaggag catggcggct gtgttggctt tgagcttgcc caatgccgct
     1501 tgggcatggc aacaagagtg tgagcctcag tttgacagct acgcgcaggt gctgaaagaa
     1561 gcgaataaag gcaatgccga agcacagtat tggttaggcg aggcgtattt cgatggtgaa
     1621 agccagtttg agcagccatc tgctgtggat tacactcagg cacgggcttg gtatgacaag
     1681 gccgtggcgc aaaaagacgc gtgcgcgcaa catgccatcg gctggatgta cgacagtggt
     1741 acaggtgcag aagaagataa agcacaagct tttgcttggt atgaaaaagc cgccaagcaa
     1801 ggcttgagca agtcacaatt caatttgggt gtgttatacc gcgatggctt gggtgtggcg
     1861 caaaacatga agtctgccaa atcatggttt gaaaaagcag cggcgcaagg cgatgaagaa
     1921 gctgtagccg aattggcaga ttggtcagaa taggcttttt aaaacatagc gtgtttgttg
     1981 aataaaacgt gcaaacaaag cggcctgtaa accttgcgag gatttacagg ccgcttgtgt
     2041 gttgtgtgct tggcttaatg gctgcgcaca aaccaccaca ccatcaataa ggcaatgagg
     2101 tttagtacca acgccaatat cgcaaacaat tgctggcctt tggcacggta agacacgttt
     2161 ttgctttgct ggcccaacag ttcagacagc acggctatcc ctatcatggc ggccatgccc
     2221 caatacacac cgtattggaa ttggcgcgca cccagcatat acatcaagat ggcaactggc
     2281 agcatggcga caatgaggat acggctagcg ggacggtaca caggatcgcg aaaatcgcca
     2341 ctggccacgc aggccaaagt taagatggat aagccgaata aaggccaagc caatccgaag
     2401 cgcaaaccca acacattttg tgcccacggc atcaatgcgc tggccaagcc gatgagggca
     2461 aaaaccgcca agagtatttt ttgcagactc atgcccaaca attgtggcgc agggcggtaa
     2521 tccgtggctt tcacatggcc agtgacatcg ccattttgtg cggcaatgta agctgcaatg
     2581 ttgttttgct tgtcgtacaa acggcggttg cctggttttt gcaacatgtt ttgtgcttgg
     2641 cgtatcaatt ctttgtcgcg gcctttgagg cgtgcattgc gtatggccac ttgaatttct
     2701 tgaaaactgg catcgccacg tatgcccaag aagtgatacc aattgtcacg cttgaattgg
     2761 ggtttgggag ctatagtggg agcaggcgtg gccttttgag cggcctgtat atcgtttttg
     2821 acacctgtat cgtcatcaaa tggctgtgcc gaatggggaa tgtccaactc attcgtttga
     2881 tgcgacgttg taggctctgt ttcttctgtt ggtgtgacag gaaccgaggt ggtgcttggt
     2941 ttggctttgc gtttaccaaa tgacagccaa gcaggcaaac gcaagcctgt gtgtggcaaa
     3001 cggctgacag gttcagcagc atccatgatg gcggcggccg attcaatgcc atgttcggca
     3061 tcgtaagcag cgcggctgtc ggggtcgagc agggtttcac gcgccaattg aatggttttg
     3121 ccattcaaac cttccagtac cgcttcatca atggcatcgg ccaattcggc ataagtggct
     3181 ttggtacgca cgcccaaaaa ttgatacaaa tgctcgtagt cgtttagctg agtggtggga
     3241 atgagtttgc tgtcgacatg gtattgcggc gcagcggttg ccacgggttc ggcagtgggc
     3301 gcggtgttgg gcataccgat ttcggcaaac agtttgcggt tgtattggtc tcgcaccgca
     3361 ggattcaaca gccattgtct tgcagccgcc acgatttttt catcggcatc ttcacgcaaa
     3421 cggtaaatag cgataccggc ctgtatcgat ttttcactgg cggtgggttc aattttgagg
     3481 atttcgtaca aatttatcat ggctgcacat cggcaattca gtgggctcca ttatgccgtc
     3541 ttttaaggca aatacccaat ttaaatgagc aagcaatgaa atgagcgttc gtgctgaggg
     3601 atgagcggtt gaattagaga atatcaccca aaccccgctt gtttatcgct ttgatatcag
     3661 cgacaatgag cacgcaaaag caaccgaatt tagggaaaac ttggtgaaaa aacaaatcgc
     3721 aatggcgctg atgttgtcag cgatgactta cggcgcatcg gctgccgaat ttatgaagcc
     3781 caataccgaa atcaacaatg ccggtaagca tgtggtgatt aacatcccac aattgcgttt
     3841 attcgtgtac gagaatggca agttggccaa atcttggccg attgcggtgg gtaaaggccg
     3901 cacccaaacg cctcctggtg agtacgatat tggtgtgaag gcttttaatc ccacgtggca
     3961 cattcccgcc agcatccaaa aagagcgtgc cagcaaaggt ttgcctgctc tgaaaaccat
     4021 cccacctggc cctaaaaatc cattaggccc tgtgtttgtg cgctttggcg atcctaagct
     4081 gggcttgggc atccatggca cgagtgcacc cgccagcgta ccgagttttg ccagccacgg
     4141 ttgtgtgcgt ttgcgcagtg aaaatgcgtt ggaatttgcc aaattcatcg acaaaggttc
     4201 acgtgtatcg gtgatttaca atgaatcggc cttgaacttg gatgcgaaca acaatttgtg
     4261 ggtgtcggct tacaaagacc cttacaactt gaaaaaaatg aatcctgccg cggtgaaagc
     4321 ccaagcacag acgattgcac aaaaatacgg caaaaccgtc aatgaaaaaa tgctgaccca
     4381 agtattggcc aaccgttctg gacaattaaa ttgtctgacc tgcgccaaag gctcagccaa
     4441 agtgcaaggt gaattgcgcc cagtgagcat gacgttcaaa acgccgcctc caccaccagt
     4501 tacagccaat ccaaccccat tgccttcttc agcgccgatt accgagccag gcgacaatgg
     4561 tgacgactac atcaagccac acaaacctgt ggattggagc agtcaattgg aagtgcgaga
     4621 ttcgtttgcc cgtttgtggt aagacagaga tgcgccaata aaaaacagga tgggtgttcc
     4681 atcctgtttt ttattgtaaa taagcggcct gtaatggttt acaggccgct tattttttaa
     4741 tggcctttta acgcgtaaat ggcagaaaga ttgcgccaag cgccgctcac atccatgccg
     4801 taaccgaaca catagcggtt gggcacatcc aagcccacat agtcggcagt gatgggtttt
     4861 tcatggtcga gcaatttgtt ggcgaacacc gcggttttgc aagatttcgc acccatggcc
     4921 atcacctgtt ctttgatgac gcgcatggtt tggccttcgt ccaaaatgtc gtccaaaatc
     4981 aacacatcgc gaccagcaat gttcttgtct tgtggtgcgc gggtccagct gaaattgccg
     5041 ccttccaatt tgtcgccgta gcgagaaacg tgtacataat cgaaatccaa agggaagcgg
     5101 agcaaaggca ataatttgcc agtgaatacc accgcgccgc ccatcactgg caagaccaat
     5161 gggtatgtgt cgcccaagtc tcgagtgatt tcagcggcca tgcgttccaa tgcctcatcg
     5221 cattgggctt cgctgaacaa caaatcagca gtgtcgagta tggattgggt ttcttcgcgg
     5281 ttgcgggtgg tcatggcgtg actccaaaat gggctcatta taccgagcta agtgggcaga
     5341 tgaaatcaat acgcgattga aaaaaagtat caatcaagac ttaaagtgtt ggcgcatgtt
     5401 cagtgagggt ttggtgcggt gtttttcaga cacaacaaca gcgtggctga gcagctatca
     5461 agcaagggta gaaatggcgc aaagcagtga agcaatgttg gcgctgatac aacccgcatt
     5521 ttagacacaa cttgttgcgt tatctctagt gttcattaca cagacgagta tatgaacaag
     5581 cattagccca gccagttagc actgcagagc gcctatcatt aagcggccag taagtctgtt
     5641 ggtggtcagc acattagtgc atcaaaattg tttgtcgctg tcgagcaata aagtcacggg
     5701 gccatcattg cacaggctca cttgcatgtg ggtttgaaac tcacccgttg ccacaatcaa
     5761 gccgtgtgct cgcagcaatt tggccaccag ctcataaaca tgggcggctt tttcagggcg
     5821 ggccgcttcg atataagaag ggcgacggcc attgcgggca tcgccataca aagtgaattg
     5881 cgacaccaat aaaaccgagc cgcctacctc caataccgac aaattcattt tgtcttcggc
     5941 atcggtaaat acccgcaaat gggcgatttt atcggcaatg tagcgggcat cactgtcggt
     6001 gtcgccatgg gccacgccca ataacaccat caaaccttgt tggatttcgc cactggtgtg
     6061 tttgctgttg gcggataaca cgtctacttg ggcatgggcc actctttgaa ccacggcacg
     6121 catggggctg ttcctttggg taacacgttc aaagcattgt aacagatgga atgaacaaaa
     6181 aagcggcctg taactgttta caggccgctt tcagggtcac aacaagcaat cagcgcactt
     6241 ggctgccaat gacgccgccc aaagctgctc cgcccaaagt agagccagtg ttaccaccga
     6301 ttaaatggcc agcagcgcca ccgatgacag cgccagcagc ggtattgcgt tgttgtctat
     6361 ccaaattggc acaacccatt aacgatgcac ctaacactcc gattaaggca aattttgcca
     6421 ccattgtttt cattgtttgc tcctaatggc tgacaccgcg attgatttgc ggctcagcga
     6481 aattgaattc tgacagacgg ttttattttc tgatgaatat ggcattttgt ctgtgtttaa
     6541 gcggttaatc atggttaaaa caggataaaa ccagtttaat ttgctcaata tcgcctgatt
     6601 gttaatcact tatgctgagt ttgactggta tttgagcaga agcgaaataa aatgcccatc
     6661 tggagtgttg gaggattagg gcttagtcag cgttgtgatg cgagacacat ggttttgtcg
     6721 atttcatagt tgtatagcca caaaaaagcc gctggctctg ttcgagcaag cggcctgtaa
     6781 acggtttgca atgcctattt ggcaggcaat accgcagggg tttcggtgac aaagcccact
     6841 ttggtcaaac ccgcttcttg cgccgcagac aagactttgg tcaaggcttc gtgttctgaa
     6901 accttgtcag cggcaatcgc caatacggtt tcagggtctt ttgccaaagc cgctttcaag
     6961 cgttggctta aggcttcagg ggtgatttct tcctctttgt tggcgcctat gtagtatttg
     7021 ccatccgcgg taatgctcaa gcgcaaaggc tctttcactt gctctttctg cgattcttgc
     7081 tgcttttctg ccgccgtagg caaattcaat ggaatcgaat gggtaagcac aggcatggtc
     7141 accatgaata cgatcagcaa caccaacatc acgtccacca gtggggtaac gttgatttca
     7201 gccatggggg tgtcgttgcc accgtcattt attgagccaa aagacatggt gttctcctag
     7261 tggtttttgt cagccaattg cgcatgcaaa tcatgggcaa agctgtcgag ttcattggac
     7321 agagttttgt tgccgcgcac caaggcgttg taagccaata ctgcaggaat ggcgacaaac
     7381 aagcccgctg cggtggccac caaagcctca ccaattgggc ctgcgacggc agcaatgctc
     7441 atttggcctg aattggaaat attgattaag gcatggtata tgccccaacc cgtgccaaac
     7501 aagccgatga aaggcgcggt ggcaccaatt gatgccaaag tggtgaggcc gctttcaaac
     7561 gggcgcaagg ctttggtcat ggcattgcgg atgtggcgta ccatgtaggc atcgaaagag
     7621 atttgtgccg acaaattgcc tttgctgctg cgctcttgga aggtgttttt gcattcaatc
     7681 gattcggtaa tcatttgtgc caacggcgaa tattgtgggt tggctgcgcg ttgagcgtct
     7741 tcaacagacg aggcttccca aaatttttcc gcttcaatgc gattggcttt cttggcctta
     7801 atcaaacggt aagagcgcca aataatgatg ctccaagtgg cgatgctcat ggccaccaac
     7861 aataagaata caaaaatcag aacaccatcg ccctgttgaa ataccaaacc taagttcatg
     7921 ttgaaacttc ctgtcttaaa taaattctgt gtttgccacg ctggggcgcg gcagcaaaaa
     7981 aaccattgct tattgtttaa tgttaaatct gtaagaggcg gcaaaactgt cttgtacagg
     8041 cacaccattt tttaccgcag gcttgaattt acaggtcgct gctttgcgtt tcgctgcctt
     8101 gtccaaacga ctggaaccgc ttgagccaac cacatccacg ctgttaatgc gaccgtctgc
     8161 acccaccacc caacgcactc tcaccacacc tgtttcgcca cggtctaagg cttcttgcgg
     8221 gtattctggg ctcaggcaat tggccgatcc tgcaattttc acattggaac ttgggccact
     8281 gccgccacct gaacctttgc cgtcattgcc acctttattg ttgtcgccac ctttgccatt
     8341 gttattggca ttggtgcctg tgccttgtgc cgcgttgcct ttgttgtctg actgctgcgc
     8401 agaagactgg ttgttggcag actgttggcg ctgattggat ttctcagtct tttgctcggg
     8461 tttgcgctca ggctcaggct tgcgttcggg cttttgttct ggtttgggct caggcttgcg
     8521 ctctaccacc gttttttcct gtggcttttt cacgatggtg cgtttgacat ctggctcagg
     8581 tttgcgctca cgtttgggct cgggtttggg ctcgggtttg gcttgagcca cgggaagcgg
     8641 tgcaggcgct ggggcaggtg cgccaccgcc aagctcgaca aaagtcaatt cttcttgcgg
     8701 tgcggccaat tcggcgggct tgtcgtcaaa attggacatg gcccatagcc cagcaatgtg
     8761 cagcaaactg acggcggtaa tggttaaggc aaagtgcgtg ttttttttca tggtggccaa
     8821 ttgtaactga gaatggt
//
LOCUS       DAOMVK010000032         8799 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_2049_length_8799_cov_2.912054, whole genome shotgun sequence.
ACCESSION   DAOMVK010000032 DAOMVK010000000
VERSION     DAOMVK010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 8799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8799
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2049_length_8799_cov_2.912054"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PK856_02095"
     CDS             <1..218
                     /locus_tag="PK856_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HRL01195.1"
                     /translation="FEHFDFLVAMDSDNLHVLEQQFGHHPDKMFVITDLVPELGYQGV
                     PDPYFTHDFQETQTLLQAACQVLLQRF"
     gene            321..686
                     /locus_tag="PK856_02100"
     CDS             321..686
                     /locus_tag="PK856_02100"
                     /inference="COORDINATES: protein motif:HMM:NF033674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NirD/YgiW/YdeI family stress tolerance protein"
                     /protein_id="HRL01196.1"
                     /translation="MRKLLLTLAMVYLSQSAFAEGFTKSVPIDSYQTISTVKAAKQQA
                     DGNTVVLEGHISDRAHTVNPEEFWFQDETGGIKIEINQNIWRGQEVNPQQKIRILGEV
                     DHNDFNNSVEIEVKQLDLM"
     gene            842..2092
                     /gene="glyA"
                     /locus_tag="PK856_02105"
     CDS             842..2092
                     /gene="glyA"
                     /locus_tag="PK856_02105"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRL01197.1"
                     /translation="MFSRSVTIEAFDPELAQAIAQEVTRQQDHVELIASENYVSPAVM
                     EAQGSQLTNKYAEGYPGKRYYGGCEYVDVAEQLAIDRIKQLFGAEYANVQPHSGSQAN
                     QAVYTCVLQPGDTILGMNLAHGGHLTHGASVNISGKLYNVVSYGLGADEAIDYDEVER
                     LAVENKPKMIVAGASAYALEIDFKRFREIADKIGAYLFVDMAHYAGLIAAGEYPNPMP
                     HAHFVTSTTHKTLRGPRGGIVLTNDEALAKKINSAIFPSLQGGPLMHVIAAKAVAFKE
                     ALDPSWKDYAKQVKANAKVLAEVLTERGLRIVSGRTESHVFLVDLRAKNITGKDAEAA
                     LGAAHITVNKNAIPNDPEKPFVTSGVRLGTPAITTRGFDENDTRELAHLIADVLDAPQ
                     DEANLKAVAAKVTALCDKRPVYGN"
     assembly_gap    3323..3332
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3333..4057
                     /gene="pnuC"
                     /locus_tag="PK856_02110"
     CDS             <3333..4057
                     /gene="pnuC"
                     /locus_tag="PK856_02110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HRL01198.1"
                     /translation="QECTTFEKIYCVAFTVTSIAISIIQDASFISALASITGMWCVFL
                     VAKGNLNNFYFGAVFLTLSGIIMWQYGLYITLAIHVGFYLPMQLVGYWFWKRSPPLYQ
                     IHHEDIVMRELFPRGWLAILIAVIISSLIYIQFLTLFNVHYSQWDSVTLIISIVGQIL
                     TTFRFVEQWVTWLCLDIFNVIWWFLVLYPQNGDLTMAVMWAFKLINVFYGWYTWKKLG
                     KAQTKLVSGPYCPYEPQFTRHY"
     gene            4120..5265
                     /locus_tag="PK856_02115"
     CDS             4120..5265
                     /locus_tag="PK856_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRL01199.1"
                     /translation="MTALRTDIDPNGLLEYSVVYTDRSLNHMSQKFQQVMRDLSATLK
                     QVYNADAVAIVPGSGTYAMEAVARQLTQDKTCLVIRNGFFSFRWSQIFDMARLPEQEI
                     VLKARQQDPNNPTSPFAPIPVEEAVAAILANKPNVVFAAHTETASGMILPDDYIRQIA
                     AATHEVGGLMVLDCIASGCIWADMKDLGVDLLISAPQKGWSAPPCCGMVMMSEAAKTA
                     VQNSQSNSFAMDLKKWLEIMQAYENGGHAYHATMPTNALEVLRDVMVESAKIGFAELK
                     QRQQTLGTRIREVMKERGVQSVAADGFAAPGVVVCFTDDDDIANTKKFIAEGVQIAGG
                     VPLKVDEPASYKSFRIGLFGLDKLEDIDRSVALFSEALDKVLAKQIA"
     gene            complement(5473..6366)
                     /locus_tag="PK856_02120"
     CDS             complement(5473..6366)
                     /locus_tag="PK856_02120"
                     /inference="COORDINATES: protein motif:HMM:NF009888.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HRL01200.1"
                     /translation="MQRFDNKQTEALLAVLDHGSFERAAQELRLTPSAVSQRVRALEA
                     QLGMPLVERTRPCRPTASGRALLQYLRRSKLLAEEFASTFADSEAFIHAPIATNHDSL
                     ETWLLPALAPVANAEKVLLQLSVDDQEFTHQKLLDGSVVAAISSKDSPMKGCEAKPLG
                     TVRYRLMASPEFQQRYFAAGLNHEALRAAPLIRYNHKDFLQHDFLYAFCHFNADASPQ
                     LTIPSTQAFYRAVELGLGYGMILDVQQQQLQGELLDLLPEAFVDVPLYWHYWKVQSPK
                     MQRLNQALIHHASAYLLPLSA"
     gene            6498..7109
                     /locus_tag="PK856_02125"
     CDS             6498..7109
                     /locus_tag="PK856_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009116239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="HRL01201.1"
                     /translation="MVSAGLIIAIGAQNAYLLKQGLSKQHVAVVAGICFLGDFCLMTF
                     GIMGAGSLFAKFPWLSLTLAIVGALFLSVYAYRCFKSAYTGASGLHLQAEQTQKQTRK
                     QVAMMALALTFLNPHVYLDTVVILGGIAGTLSIADKWSFLAGALLASLTWFFGVGYGA
                     RLLLPWFAKARTWRMLDTGIGLLMLWLAIGLYRYAYTLSTASA"
     gene            complement(7191..7694)
                     /locus_tag="PK856_02130"
     CDS             complement(7191..7694)
                     /locus_tag="PK856_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01202.1"
                     /translation="MHGILDEVPHTLRSIHTNATHLMLATGQAAQTPYVCHVPYVWHE
                     DAYCVWIADVTEPIRHLRLAQAASVMLLEHGHGQQSTQLIWIVEASEVAYQSPQYREL
                     LQQMCLHSNVDLQAALAAECQAIYRLKPIKGRLITDLNEIIALIAKDLHEAMAITQQK
                     TLAIANV"
     gene            complement(7872..8756)
                     /locus_tag="PK856_02135"
     CDS             complement(7872..8756)
                     /locus_tag="PK856_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRL01203.1"
                     /translation="MSSYKIKTINTFFKRAVSVLERLDAWLPQPLQEPNWAAVAFRWQ
                     QVGQQGILQALPTPHTFPLSRLAAVDTQMKQLVRNTEQFLAGRPANNALLSGARGTGK
                     SSLVKALLHEYAEQGLRLIEVDKQDLASLPSLLKLLQDRPEKFIVFCDDLSFEHGDDG
                     YKALKTALDGGLSVRAENVIVYATSNRRHLLPEYFADNTGYTGDKGEVHPQEAVEEKV
                     SLSDRFGLWLSFYPFDQIAYLQAVENWLQAADVVFDETARKAALNWSLSRGGRSGRVA
                     WQFVCDWVGRLPEERIVD"
BASE COUNT         2359 a         2203 c         1951 g         2276 t
ORIGIN      
        1 attttgaaca ttttgatttc ttggttgcga tggatagcga taatttgcat gtgctggaac
       61 aacaatttgg ccaccatccc gacaagatgt ttgtcattac cgacttggtg cccgaattgg
      121 gttaccaagg cgtgccagac ccttacttca cccatgattt ccaagaaacc caaactttgt
      181 tacaggccgc ttgccaagtg ttgctacagc gtttttgagt tcgctttcca aaaacaaata
      241 ccgaacttgt gcgatttttc atggcaacgc cgctgatatc tctacaatcg acacttcctc
      301 aaccctctgt tggaatccac atgcgcaaac ttttgcttac ccttgctatg gtctatttaa
      361 gccaatcggc atttgctgaa ggtttcacca agtcagtacc tattgatagc taccaaacca
      421 tttctaccgt caaagccgcc aagcagcaag ccgatggcaa cacagtcgta ctggaaggcc
      481 acatttccga ccgtgcccac accgtcaatc cagaagagtt ttggtttcaa gatgaaacag
      541 gcggcatcaa aatagaaatc aaccaaaaca tttggcgagg ccaagaggtc aatccccaac
      601 aaaaaatccg tattttgggc gaagtcgatc acaatgattt caataacagc gtggaaattg
      661 aagtgaaaca attggactta atgtgagaaa tcagaggcat tggcgagttc ctgtcacatt
      721 cagtcggctt taagctattt tcacgtcaat acgggcatgc acatccagca aatatcggct
      781 agaatggcgc cttactccta ggctctgcct tattttacgc actctcattg gaaggatcgt
      841 catgttctct cgcagtgtta ccatcgaagc ttttgaccct gaattggccc aagccattgc
      901 tcaagaagtc actcgtcaac aagaccatgt tgaattgatt gcatctgaaa actacgtgtc
      961 tcctgcagtg atggaagcac aaggctcaca attgaccaac aaatacgccg aaggctatcc
     1021 tggcaagcgt tattatggcg gctgtgagta cgtggatgtg gcagaacaat tggccataga
     1081 ccgcatcaag cagttgtttg gtgcagaata cgctaacgta caacctcact caggctcaca
     1141 agccaaccaa gcggtttaca cctgtgtatt gcaaccaggc gacaccatct tgggcatgaa
     1201 cttggcacat ggtggtcact tgacccacgg tgcatcagtg aacatttctg gcaaattgta
     1261 caatgtcgtg tcttacggct tgggagcaga cgaagccatc gactatgacg aagtagagcg
     1321 tttggccgtt gaaaacaaac ctaaaatgat tgtggcgggt gcttcagctt acgccttgga
     1381 aattgatttc aaacgtttcc gtgaaattgc cgacaaaatt ggtgcttatt tgttcgtgga
     1441 catggcccac tatgctggtt tgattgctgc aggtgaatac cctaacccaa tgcctcacgc
     1501 gcatttcgtc acttctacca cccacaaaac cttgcgaggc cctcgtggcg gcatcgtgtt
     1561 gaccaatgac gaagccttgg ccaaaaaaat caactcagcc atcttccctt cattgcaagg
     1621 cggcccgttg atgcacgtga ttgcagccaa agccgtggca ttcaaagaag ctttggatcc
     1681 aagctggaaa gactatgcca aacaagtcaa agccaatgcc aaagtgttgg cagaagtatt
     1741 gaccgaacgc ggcttgcgta ttgtttcagg ccgcactgaa agccatgtgt tcttggtgga
     1801 cttgcgtgcc aaaaacatca cgggcaaaga cgcagaagcc gctttgggtg ctgctcacat
     1861 caccgtgaac aaaaatgcca tccctaacga ccctgaaaaa ccatttgtga cttcgggcgt
     1921 gcgtttgggc actccagcca tcaccacccg tggtttcgat gagaacgaca cgcgtgaatt
     1981 ggcacatttg atcgcagatg tgttggatgc accgcaagac gaagcaaact tgaaagcggt
     2041 tgcggccaag gtcactgctt tgtgcgacaa acgtcctgtt tacggcaact gattaaaaaa
     2101 ccattaaata tcaaaagcgt cctgaattta gggcgctttt tttaggtgat tttaggcaca
     2161 aaacaaaccg attaaagtgc aaagtctagc tatatttttg ctgagattga tgcattttgc
     2221 ataatggcat aattaatgct tgcctaaaat acacacacga caccttcaca cagtgtcaca
     2281 ccacacacaa ggttacaaaa tgcaatcatg gaaagagtgc acctggctcg aagggctgtg
     2341 ggtgttgttc ttcacatcac tgtttttgcg gctctgcggt tttgtctcaa tacgcacatt
     2401 gtcagcacgc tttgctgaca tcgcctatcg tttcaatact tggcaagcgc attcgtgcag
     2461 gaaaaacata cttttcaacc actcacggcc gtctctgaaa cgaacgccac gcaactctca
     2521 atgccatgat tcaccgcggc cctaccgtct tcctcttgtt attcttcatt taagactgct
     2581 tcatacacac agatacctgc agtcatacac cgtgaaacca tctgtggcat ataaaaatac
     2641 agctcatgat gttcgtttgc cctcaccaaa gtatgcatgg tcacaagaga cccatgagat
     2701 cccacgccac accacaataa gcattactca gccaaaagcc tgctatgacc aattatctgg
     2761 cattccagtg tttacaggcc gcttattaaa gcttgaaatg agcctcaatc ttaggctctt
     2821 aacacaaatg gcaagattgc ccagcagcat caaaaaatcc ctcacaaatc gcttgctaag
     2881 aaagttgggt acagtgctca acggtttaaa atccttaaga ttgagattcg ataaaaaaca
     2941 attcgcaatc tgtttggctc tgttgcaacc gccttggctt tgtcacactt ttttgactta
     3001 acacaatata ttattatcat taagcaaata gcataattta cggcattagg tttgctttta
     3061 aacatggtaa gaacttatat agaatcaata caaaactggc tttaagtact gaaaatattt
     3121 tcattttaat aatgaagaaa tacctttaag tgacagcttc ttaataaaat aataaaaaat
     3181 accaagcaaa agctctgttt gatttttaat tttacaacaa aacaaacggt ttatttctga
     3241 cttaaatgga cgttttttgg cactcttgaa aagcacaatg aaagctcaat aatgagattt
     3301 ttggataaaa tcgcctgatt ttnnnnnnnn nnggcaagaa tgcacgacat tcgaaaaaat
     3361 atactgcgtg gcctttacgg tcaccagcat tgccatatcc atcattcaag atgcttcctt
     3421 catcagtgcc ttggcatcga taacaggcat gtggtgtgta tttttggttg ccaaaggcaa
     3481 tttgaacaac ttctattttg gcgccgtctt cctcaccctg tccggcatca tcatgtggca
     3541 atacggcctg tacattacgc tggccataca cgtaggcttt tatttgccca tgcaattggt
     3601 tggttattgg ttttggaaac gctcgccgcc tttgtaccaa atacaccatg aagacatcgt
     3661 gatgcgggag ttgtttccac gcggatggtt ggctatcttg attgctgtca ttatcagctc
     3721 attgatttac atccaatttt tgactttatt caatgtgcac tattcgcagt gggacagcgt
     3781 taccctcatc atcagcattg tggggcaaat tctcaccaca ttccgattcg tggaacaatg
     3841 ggtcacttgg ctgtgcttag acattttcaa tgtgatttgg tggttcttgg tgttgtatcc
     3901 ccaaaatggg gatttgacca tggccgtcat gtgggcattt aaactcatca atgtcttcta
     3961 tggctggtac acgtggaaaa aattgggtaa agcacaaacc aaattggtat caggacctta
     4021 ctgcccatat gagccccaat tcactcggca ttattgacat gacaaacacc ccataagccg
     4081 ttaagctgtc atctcgacac tcatgattaa ggactgaaga tgactgcttt gcgcaccgat
     4141 atcgatccca atggtttgtt ggaatattca gtggtgtata ccgaccgctc actgaatcac
     4201 atgtctcaaa aattccaaca agtcatgcgg gatttgtctg ccaccttaaa acaagtctat
     4261 aacgccgatg cagtggccat cgtacctggt tctggcactt atgccatgga agccgtggcg
     4321 cgccagttga cccaagacaa aacctgcttg gtcatccgca atggtttctt cagttttcgc
     4381 tggtcacaaa tttttgacat ggcacgtttg cccgaacaag aaattgtctt gaaagcacgc
     4441 caacaagacc caaacaatcc cacctcccca tttgccccca ttccagtcga agaagccgtt
     4501 gccgccattt tggcgaacaa acccaatgtg gtgttcgcag cccacaccga aaccgcttct
     4561 ggtatgattt tgcccgatga ttacatccgc cagattgcgg ctgccaccca tgaagtcggt
     4621 ggcttaatgg tgctagactg cattgcctca ggctgtattt gggcagacat gaaggacttg
     4681 ggtgtggatt tactgatttc agcgccgcaa aaaggctggt ctgccccacc ttgctgcggc
     4741 atggtgatga tgagtgaggc cgccaaaaca gcagtgcaaa acagccaaag caacagtttt
     4801 gccatggatt tgaaaaaatg gctggaaatc atgcaagcgt atgaaaatgg cggccatgcc
     4861 taccacgcca ccatgcccac caatgcctta gaggtcttgc gtgatgtgat ggtggagtcg
     4921 gccaaaattg gttttgccga attgaagcag cgccaacaaa ccttgggcac acgcattcgt
     4981 gaggtgatga aagaacgtgg tgtgcaaagc gtggcagcag atggttttgc cgcaccaggc
     5041 gtggtggtgt gtttcaccga tgacgacgac attgccaaca ccaaaaaatt catcgccgaa
     5101 ggcgtgcaaa tcgctggcgg tgtacctttg aaagtcgatg aacccgcctc ttacaaatcc
     5161 ttccgcatcg gcctgtttgg tttggacaaa ttagaagaca tcgaccgctc agtggcattg
     5221 ttcagcgagg ctttggacaa agtattggcc aaacaaatcg cgtaatcctc acatgcagcc
     5281 tgtaatgcat aggcaagaat cgtaaaaccc acacaagcgg cctgtaaacc aacattgcgt
     5341 ttacaggccg cttattaatg ctcgcagcag cccgttaaga tttaaaacca ctcgtcgatt
     5401 taatacacgc gttactcgcc aatttaaatg actgaaaccc tacaagcggc ctgtaaacat
     5461 caatacccaa catcaagcac tcaaaggcaa caaataagcg ctggcatgat gtatcaaagc
     5521 ctgattcaag cgctgcattt ttggcgattg cactttccaa taatgccaat acaaaggcac
     5581 atccacaaaa gcctcgggca gcaaatccaa tagttcaccc tgcaattgct gctgttgcac
     5641 atccagtatc atgccgtagc ccaaacccag ctccaccgcg cggtaaaagg cttgggttga
     5701 tgggatggtc aattgcggac tggcatcggc attgaaatgg caaaaagcgt acaaaaagtc
     5761 gtgctgtaaa aaatctttgt ggttgtaacg tatcaatggc gccgccctca gcgcctcatg
     5821 gtttaagcca gctgcaaaat agcgttgctg aaactctggg ctcgccatca aacggtaacg
     5881 caccgttccc aagggcttgg cctcacagcc tttcatcggt gaatctttgg atgaaatggc
     5941 cgccaccacc gaaccatcga gcaatttttg gtgggtaaac tcttggtcgt ccacactcag
     6001 ctgtagcaag actttttcag cattggccac aggtgccaat gcgggcaata accaggtctc
     6061 caaagaatcg tggttggtgg caatcggcgc atggatgaag gcctcgctgt cggcaaatgt
     6121 ggaggcaaac tcttctgcca acagtttcga gcggcgcaag tattgcaata aagcgcggcc
     6181 tgaagcggtc ggccgacaag gccttgtgcg ctccaccaaa ggcatgccca attgcgcctc
     6241 caaagcccgc acccgttgcg acaccgctga aggggtcaaa cgcaattctt gcgccgcacg
     6301 ttcaaaactg ccgtgatcca aaaccgccaa taaggcttcg gtttgtttgt tgtcaaatcg
     6361 ctgcatggcc gcacacaaat gaagaaaaat ttaatcttac taaaatttat tcagttttgt
     6421 ttcatttatt tcaccaaact ttgataatgc ccacacacaa caaagggaat acggtcatgg
     6481 ttttttagca tggattgatg gtgagcgcgg gtttaatcat cgcaataggg gcgcaaaatg
     6541 cgtatttgct caaacaaggc ttgagcaagc agcatgtggc tgtggtggcg ggcatttgtt
     6601 ttctgggcga tttttgcctg atgacgtttg gcatcatggg cgcaggcagc ttgtttgcca
     6661 aattcccatg gctaagcttg acccttgcca tagtgggggc gttgttttta agcgtgtatg
     6721 cctaccgctg ctttaaaagc gcctacacgg gtgcctctgg cttgcattta caggccgaac
     6781 aaacccaaaa gcaaacgcgc aaacaagtgg cgatgatggc tttggcctta acctttctca
     6841 atccgcacgt gtatttggac acggtggtga ttttaggcgg catcgcggga acactcagta
     6901 ttgccgacaa atggagtttc ttagcaggtg ccctactggc ttcacttact tggttttttg
     6961 gcgtgggtta tggcgcccgc ttgctattgc cttggtttgc caaagcccgc acttggcgca
     7021 tgctggacac gggcatcggt ttattgatgt tatggttggc catcggcctg taccgctacg
     7081 cttacacact gagcacagct tcagcttgaa cagactatca atgcctttaa cgcaaaaaaa
     7141 cgcccccgac atcgtcgggg gcaaacggaa cacacctcat aaactctgta ctaaacgttt
     7201 gctatagcaa gcgtcttttg ttgggtaatc gccattgcct catgcaaatc cttggcaatc
     7261 aacgcaataa tctcattcaa atcggtaatc aagcggcctt ttatcggttt caagcggtaa
     7321 atggcttggc actctgccgc caaagcggcc tgtaaatcca cattgctgtg cagacacatc
     7381 tgttgcagca actctctgta ttggggcgac tgatacgcca cttcgctggc ttccacaatc
     7441 caaatcaatt gtgtggactg ctggccatga ccatgttcca acaacatcac actggctgct
     7501 tgggccaaac gcaagtggcg tatgggttca gtcacatcgg ctatccacac acagtaagcg
     7561 tcttcatgcc acacatacgg cacatgacac acataaggtg tttgtgctgc ttgccctgtc
     7621 gccagcatca aatgcgtggc atttgtgtgt atgctgcgta aggtgtgtgg gacttcatcc
     7681 aatataccgt gcatgtttgc ctcccctcgc tcgttcgttt taagcttgag tgcatcataa
     7741 tccagtcgat ttattttgta aatgataatt attactattt actaataaaa accgcctcat
     7801 ttccatactt tattgttttt aataataaaa aaatccagcc taagctggat tgtttacatt
     7861 ctttgcaaaa ctcagtcaac aatacgctct tcaggcaaac gccctaccca atcacacaca
     7921 aattgccacg ccacacggcc agaacgaccg ccgcggctga gtgaccaatt caaagcggct
     7981 ttgcgggcag tttcatcaaa caccacatca gcggcctgta accaattttc cactgcttgc
     8041 aaatacgcga tttggtcgaa tgggtaaaaa ctcagccaca aaccaaagcg gtcggataaa
     8101 gacactttct cctccaccgc ttcttgcggg tgcacttccc ctttgtcgcc tgtgtaaccc
     8161 gtgttgtcag caaaatactc gggcaacaaa tgacggcgat tgctggtggc atacacaatc
     8221 acattctcag cccgcaccga caagccacca tccagcgcgg ttttcaaggc tttgtagcca
     8281 tcatctccgt gctcaaacga caagtcatca cagaacacaa tgaatttttc aggccggtct
     8341 tgtagcaatt tcagcaagct tggcaaagaa gccaaatctt gtttgtccac ctctatcaag
     8401 cgcaaacctt gctcggcgta ttcgtgcaac aaggctttta ccaaagagga tttgcccgta
     8461 ccacgcgcgc ctgaaagcaa ggcattgtta gcgggacgac ctgccaagaa ttgctcagtg
     8521 ttgcgcacca attgcttcat ttgcgtgtcg acagcggcca aacgcgataa aggaaaagtg
     8581 tgcggtgtgg gcaaggcttg caaaatgcct tgctgcccca cttgttgcca acgaaacgcc
     8641 accgccgccc aattaggttc ttgcaaaggc tgtggcaacc aagcgtccaa acgctccaat
     8701 accgataccg cacgcttgaa aaacgtgttg atggttttaa ttttgtaact gctcatggtt
     8761 tcctttcagg ccgcagctta agcgcggtgc tggtgtgtg
//
LOCUS       DAOMVK010000033         8769 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_2057_length_8769_cov_3.224352, whole genome shotgun sequence.
ACCESSION   DAOMVK010000033 DAOMVK010000000
VERSION     DAOMVK010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 8769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8769
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2057_length_8769_cov_3.224352"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..67)
                     /locus_tag="PK856_02140"
     CDS             complement(<1..67)
                     /locus_tag="PK856_02140"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:NF020132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HRL01204.1"
                     /translation="MTIAGSDSGGGAGIQADLKTFQ"
     gene            complement(76..831)
                     /gene="thiM"
                     /locus_tag="PK856_02145"
     CDS             complement(76..831)
                     /gene="thiM"
                     /locus_tag="PK856_02145"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HRL01205.1"
                     /translation="MSLNHIRRHAPLVHCISNIVVANFKANGLLAIGASPVMADAPEE
                     VAQMVAIAGVLSINMGTLNSESVKAMLAAGRAANALNKPVVFDPVGAGATEFRRNTVR
                     ELMREVKLSVLRCNVGELAAVAEVPWQQKGVDSGEGDLAIVEVAKQVAKQYHTVVAVT
                     GETDIVTDGEHVAEISGGSAMTPLVTGTGCLLSAVVAASLVGSDDVFAATVSVLQDYK
                     RVAEIAAAEGKQVGDFQVAFLNALQKVSEGEGA"
     regulatory      complement(914..1014)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1135..2064
                     /locus_tag="PK856_02150"
     CDS             1135..2064
                     /locus_tag="PK856_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01206.1"
                     /translation="MMLNLYALLGVSEIATAANIAEAIDTAEKQGEFSSKVLQSARFY
                     LLQVDKRTEYNRALSRAKQKGTVTTDWQAALAESERKQAMPATVSPKQNTVAVYQQAS
                     GFGSTPAAHIPMPEETTHKMGKRHWLALLLCVLITGVLVAVLWQMAQVQLHQNEQITA
                     LQKMTAERRLPEDWPTWEQLDGETFLSAKEQANTKLTIYKNATQDLPVMLLPSSKRID
                     CGERGNQSCAVTLAFDGSNPRTFEAQIVGSSLVFNDMKQLPVLMHELSGASSIAASFP
                     KAKDMTTLTFERPNPTGSASEPKNSLKNKAQKG"
     gene            2067..3035
                     /locus_tag="PK856_02155"
     CDS             2067..3035
                     /locus_tag="PK856_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HRL01207.1"
                     /translation="MFDFQFLGTSAGIPTKTRNVTALAIGQTASKPWLLVDCGEATQQ
                     QLLHTPWSANTLSAICITHVHGDHCYGLPGMLASAGLNKRTQPLTIIAPLHLWQWLQH
                     TIDLTDLFLSFELQFVNVTSLLNQSVTVDGFEISAVALNHRVPSYAYKISHQRQVQQL
                     DRDALFAHGLPAGPLWGMLQNGQDVYYEGRNYPASLYLEQHEQQVCALIGGDNDTPAL
                     LQAACDGVQVLVHEATYTQAVADKVGAFPMHSSAKQTAEFAYQAAIPNLVLTHFSARY
                     NNAEALEPLLNEAKRHYHGHVHLAEDFAQYRLLPTGEFQLLTSRDA"
     gene            3047..3883
                     /locus_tag="PK856_02160"
     CDS             3047..3883
                     /locus_tag="PK856_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01208.1"
                     /translation="MINLYALLNLGPYVDAPSIEAAIGKNRQTGSINAAILDKAEQWL
                     LNPAIRNRYDAQLKQQQPEFFLATPATDTWKLDDGEDEVRLGVLEEQKADGVHLETAT
                     QFDNLVYIHEQNRYNLLTKTIIYGGIAMAILGIVLAIAWPMLKKTVFATSDWDKQQYL
                     QQFVQKGWQEDAEMGEVYLESEEYTSRLAVKYKDNDAVAVLTNTSCDMSKKGLCTIRI
                     EVDGQVLNNDADVGVGLMDDMPLATRADRESTLERLRAAKKIKVSYLSQGEPITQTFQ
                     TP"
     gene            complement(3942..5345)
                     /locus_tag="PK856_02165"
     CDS             complement(3942..5345)
                     /locus_tag="PK856_02165"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRL01209.1"
                     /translation="MDKPGNYYQSINRVLFHQIPETAMRILEIGCASGMFGAALKEQN
                     PQRYVVGVEYVPAVAEVAKIRLDEVYAFDIQVQDLPDSIALQSFDCIVFGDVLEHLTE
                     ATSVLRKLLPYLKPDGQVVACIPNVQHFSVIQQLLTQNFQYHGAGLLDKTHVNLMTLP
                     NMFKMFLDAGYLPQLKQVIHNGAIDEGFMNSLNPVLGYLGIAPERAKLTLEAFQYVLS
                     ARIQQANLNRLAAWQNKVKPQACTVAVAANNALQYNNNIAASPELARNDRQHQLLPIT
                     GARHMAEAWQYALAHAEHNWIVLVHQDVYLPAGWLEQVQYAWHEAEEKLKQQVAVLGV
                     AGVSYIKDADFCQLGSCVDRVMTFAPSAALPELCQSLDDMVLVFRKDRFLPELSADLG
                     FQFYGTDAVMRAQEAALNAVVVDLPCFSNSSRVENHMQDDDLKQKALAFHRLWQHRLP
                     VFTLVAQFQPEQIVFNH"
     gene            complement(5419..6759)
                     /locus_tag="PK856_02170"
     CDS             complement(5419..6759)
                     /locus_tag="PK856_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003770376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HRL01210.1"
                     /translation="MVNETLNIAVVGHTNTGKTSLLRTLLRDEAFGEVKNAPATTRHV
                     EVAEITHQGQTLVKLFDTPGLEDAGGVLDWLEQHTSSRSDGIERLQQLLQADVAQHEF
                     SQEAKVLRQVLASDVSLYVIDAREPVLAKYKDELTVLSWCAKPVMPVFNFTEQGDASA
                     WHDMLARRGLHVHSQFDTVAFDFDGEMRLWKQLDILLNRKPVLEQLQTVRTLEWQALQ
                     DTAWALMANFLLEVAAFVETIKDEDAVEPVLAQMQNKVRQRERHFQQDLLQAFRFYQQ
                     ADLPADWMPKAFSQDPFDPELLKQFGLRTSTGAAVGALLGLGVDAATAGLSGGMGMAL
                     GGVIGGVLSNMGSLSDKLSGVKTIHIDPATITLLATRAQALILALLQRGHAAQQTLSL
                     QAADVIWPNSNLPGPLRRARSHSEWSALNGVKASKAESNRREAAQELQRNLRRS"
     gene            complement(6749..8062)
                     /locus_tag="PK856_02175"
     CDS             complement(6749..8062)
                     /locus_tag="PK856_02175"
                     /inference="COORDINATES: protein motif:HMM:NF022518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HRL01211.1"
                     /translation="MIHLHHAYIELIRRLEEQHFVFPSDPARINAELKHTDADFDSKL
                     WLRAQRIDSDQHVHTMLQQYTGRAVLLRQILWVMWLVLGIFTAVGLMQTPSLNFFYVL
                     VSVLGLNALMFVAWLWYLLKPNTHVPAWLQAGLGLGAQRKPEHQALFALLREQLAQPQ
                     MKWWVGCISHGMWLASLGGLLLGVVVMLLVRQYTFSWQSTLLDQGSLNVVVAALAWLP
                     SVLGSNVPDAHTVAASQSVGEVAFSRQWANLLWSSLLVYGLLPRLLAWLWCRWRLRAY
                     SPSLPLNLPYYQRLKRLWQTHIVDNSNDYRADAKREQTRPVQTAAQTVFAAWESLPCA
                     ALLTKMRPVNAQDQGVIDSRTEQEALLQHLQAAPARLVLAVRLQGLPDRGSMRRFDAY
                     ADAAQGGMLVCLWGEGSDGERLRLWQQALNEQAIEWQWMDSNDGE"
     gene            complement(8165..>8769)
                     /locus_tag="PK856_02180"
     CDS             complement(8165..>8769)
                     /locus_tag="PK856_02180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HRL01212.1"
                     /translation="GRWQHQNLWLNGQVYYANLDVDSHRRVQLGAAERIHHGSADGDQ
                     YGGKISAGMNWQQGKISHGPVLSLSAQKATVKSLREQGKLSTAMSFDEQEHTSIQSGL
                     GYQAQWAVNDAWQVYGSAQWQHEFKDVDDVVGARLNSVDDMHFVLPVNTGDAKNSGVL
                     ELGVNGNISEHWQVGAGVLAEVGDAPNAQTSVFVNTSYRF"
BASE COUNT         2302 a         2473 c         1964 g         2030 t
ORIGIN      
        1 cttgaaacgt cttcaaatcg gcctgtatgc ctgcgccgcc gcccgaatcc gagcctgcaa
       61 tggtcatggt cacattcatg cgccttctcc ttcagacact ttctgtaagg catttaaaaa
      121 cgccacttga aaatcgccca cttgtttgcc ctcggcagcg gcgatttccg ccacacgctt
      181 gtagtcttgc aacactgaca cagtggccgc gaaaacatcg tcactgccca ccaagcttgc
      241 tgccactacc gcactgagca aacagcccgt gcctgtgacc aaaggtgtca tagccgagcc
      301 gccactgatt tcggcgacat gctcgccatc ggtaacgatg tcggtttcac ccgttactgc
      361 caccacggtg tggtattgct tggccacttg tttggcgacc tcgacaatgg ccaaatcgcc
      421 ttcgccgctg tccacgcctt tttgttgcca tggcacttcg gccacagccg cgagctctcc
      481 aacattacaa cgcaacacac tgagcttcac ttcgcgcatc agctcgcgca cggtattgcg
      541 gcgaaactcg gtggcacctg cgcccacagg gtcaaatacc acaggtttat tcagcgcatt
      601 ggcagcgcgg cctgcggcca gcatggcttt gacagattcg ctgttgagcg tgcccatgtt
      661 gatggacaat accccagcga tggccaccat ttgcgccacc tcttcaggcg catcggccat
      721 cacaggtgag gcaccaatgg cgagcaagcc attggctttg aaatttgcca cgacaatatt
      781 ggaaatacaa tgcaccaaag gcgcatggcg acggatatga ttcaagctca tgttggttac
      841 cttgaaataa agacaaaaaa ccaacaccgt gcaaagtggt gagtcatgcg gcctgtaaat
      901 caattaaaac tttacagata agacgcttgc ttcctacgcc ggtattaacc gacaggttca
      961 aagggtcaga cgggtgtctt tctcaacgcg aagtgcgttc ccctgcaacg atgtgatgga
     1021 cgaaactata acactgtggc acaaacttgt cagtgtcgag tatcatgtca ggacacgctt
     1081 ggaatgcgtg tgtgtatcct acctgactca caatcacacc agaataatat caatatgatg
     1141 ctgaatttat atgcgctatt gggtgtgtca gaaattgcca cggcggccaa cattgccgag
     1201 gccatagaca cagccgaaaa acaaggggaa ttttcaagta aggtgttgca atcggcacgc
     1261 ttttacttgt tacaagtcga caaacgcacc gaatacaatc gggctttgtc gcgtgccaag
     1321 caaaaaggca cggttaccac cgattggcaa gcagctctgg ccgaaagcga gcgcaaacaa
     1381 gccatgcccg ccacagtgtc gccaaaacaa aacactgttg ccgtttatca gcaagccagc
     1441 ggctttggtt ccactcctgc tgcccacatc cccatgccag aagaaaccac ccacaaaatg
     1501 ggtaaacgtc actggctggc cttgctgttg tgtgtattaa tcacaggcgt tttggtggca
     1561 gttttgtggc aaatggcaca agtacaattg caccaaaatg aacaaatcac cgccttacaa
     1621 aaaatgacgg ctgaacgacg cttgcctgaa gattggccca cttgggaaca actggatggt
     1681 gaaacctttt taagcgccaa agagcaagcc aataccaaac tcaccatata caaaaatgcc
     1741 acccaagatt tgcctgtgat gctgttgcct tcaagcaaac gcatagattg tggcgagcgg
     1801 ggcaatcaaa gctgcgccgt caccttggct tttgatggca gtaacccgcg tacgtttgaa
     1861 gcacaaatcg ttggctccag cctcgttttc aacgacatga aacaattgcc cgtattgatg
     1921 cacgagttga gcggcgccag cagcatcgcc gccagtttcc ccaaagccaa agacatgacc
     1981 accctcacgt ttgagcgccc caatcccact ggctccgcca gtgaaccaaa aaacagcctc
     2041 aaaaacaaag cacaaaaggg ttaaacatgt ttgattttca gtttttgggc acttctgcgg
     2101 gcatccccac caaaacccgc aacgtcaccg ctttggccat aggtcaaaca gctagcaaac
     2161 cttggctgtt ggttgattgt ggcgaggcga cgcaacaaca gcttttacac acaccatggt
     2221 cggccaacac cttgagtgcg atttgcatca cccatgtaca tggcgaccat tgctacggtt
     2281 tgcctggcat gctcgccagt gccggcttga acaagcgcac ccagccttta accattatcg
     2341 caccactaca tctgtggcaa tggctgcaac acacgataga tttaaccgat ttgtttttgt
     2401 cgtttgaact gcaatttgtc aacgtgacct ctttgcttaa ccaaagcgtg acggtggatg
     2461 gctttgaaat ctcagcagtg gcactgaacc atcgcgttcc gagttatgcc tacaaaatca
     2521 gccatcagcg ccaagtgcaa caactcgacc gcgatgcctt gtttgcacac ggtttgcccg
     2581 caggcccatt gtggggcatg ttacaaaatg gtcaagatgt gtattacgaa ggacgcaact
     2641 accctgcttc gctgtatctc gagcaacacg agcagcaagt atgcgcctta attggcggcg
     2701 acaatgacac gcctgcattg ttacaggccg cttgtgatgg ggtacaagtg ttggtgcacg
     2761 aagccactta cacccaagcc gtggctgaca aagtgggcgc tttccctatg cacagttcgg
     2821 ccaaacaaac cgctgaattc gcataccaag ccgccattcc caatttggta ttaacccatt
     2881 ttagtgcccg ttacaataat gccgaagcct tagagccact tttgaatgaa gccaagcgcc
     2941 attaccacgg ccatgtgcat ttggccgaag acttcgccca atatcgcttg ttgcctaccg
     3001 gcgaatttca actattgaca tcgagagacg catgagccca tcaccaatga ttaatttgta
     3061 cgccttgctg aacctcggcc cttatgttga cgcgccaagc attgaggctg cgataggcaa
     3121 aaaccgccaa acaggcagta tcaatgctgc aatattggac aaggcagaac aatggctgct
     3181 caaccctgcc atacgcaacc gctatgatgc tcaattaaaa caacagcaac cagaattttt
     3241 cttggccacc cctgctaccg atacatggaa actggacgat ggcgaagacg aagtcagact
     3301 gggcgtattg gaagagcaaa aagccgatgg cgtgcacttg gaaaccgcca cacagttcga
     3361 taatttggtc tacatccatg agcaaaaccg ttacaacctg ctgaccaaaa ccatcattta
     3421 tggcggcatt gccatggcga tactgggcat cgtgttggcc atcgcttggc ccatgcttaa
     3481 aaaaaccgtg tttgccacca gtgattggga caagcagcag tatttacagc agtttgtaca
     3541 aaaaggttgg caagaagatg ccgagatggg tgaagtgtat ttggaatcag aagaatatac
     3601 ctctcgcttg gccgtcaaat acaaggacaa cgatgctgtc gcggttttaa ccaacacctc
     3661 ttgcgacatg agtaaaaaag gcttgtgcac catacgcatc gaagtcgatg gtcaagtctt
     3721 gaacaacgat gcagatgttg gtgtgggcct catggacgac atgccattgg cgactcgcgc
     3781 tgacagagaa tccactttag agcgtttacg ggccgctaaa aagattaagg tttcttattt
     3841 gagtcagggc gaacccatta cccaaacctt ccaaacgcct taaaacaaaa cagctgctca
     3901 ctgaattcgg tgagcagctg cttggctttg ttttgatacc gtcaatggtt aaaaacaatt
     3961 tgctcaggtt gaaactgcgc caccaaagta aacacaggca aacggtgttg ccacaaacgg
     4021 tgaaaagcca aggctttttg ttttaaatca tcatcttgca tatgattttc aacccgagaa
     4081 gaattgctaa aacaaggcaa gtccaccact acagcattca aggccgcttc ttgagctcgc
     4141 atcactgcat cggttccata aaattgaaaa cccaaatcgg cagacaactc aggtaaaaag
     4201 cggtctttac ggaacaccaa caccatgtca tccaaagatt gacataattc tggcaacgca
     4261 gccgatggtg caaaagtcat caccctatca acacatgacc ccagttggca aaaatcggca
     4321 tctttgatgt agctcacgcc tgccacaccc aataccgcca cttgttgttt gagtttctct
     4381 tctgcttcat gccaagcata ttgcacttgt tctagccatc cagctggcag gtacacgtct
     4441 tgatgcacca acacaatcca attgtgctcg gcatgtgcca gcgcatattg ccaagcttca
     4501 gccatgtggc gcgcacctgt gattggcagc aactggtgtt gtctgtcatt gcgagccaat
     4561 tccggtgaag ccgcaatatt gttgttgtat tgcaaagcat tgttggcagc caccgccaca
     4621 gtacacgctt gtggcttgac tttattttgc catgccgcca atcgatttag attggcctgt
     4681 tgtatgcgtg cactgagcac atattggaat gcttccagtg tcagcttagc ccgctcaggt
     4741 gcaatgccca aatatcccaa cactggattc aagctgttca tgaaaccttc atctattgca
     4801 ccattgtgta tgacttgctt gagttgcggc aagtacccag cgtctaaaaa cattttgaac
     4861 atattgggca aagtcatcaa attgacatgg gttttgtcca acaaacccgc accatggtat
     4921 tgaaaattct gcgtcaacaa ctgttgtatc accgaaaaat gctgcacatt gggaatacaa
     4981 gcaaccactt ggccatcagg ttttaaataa ggcaacaatt tgcgtaatac cgacgtggct
     5041 tcggttaaat gctccaacac atcaccaaac acaatgcaat caaacgattg caaagcaatg
     5101 ctatcgggca agtcttggac ttggatatca aatgcataca cttcatccaa acgtattttg
     5161 gccacttcgg caacagcagg cacatactcc acacccacca cataacgctg tggattttgc
     5221 tcttttaagg ccgcaccaaa catgccactg gcacaaccaa tttccaaaat gcgcatggca
     5281 gtttcaggta tttggtgaaa caacactcgg ttgatgcttt gataataatt gcccggttta
     5341 tccatgaaat tcccttaatt tttggtgtag cctagtcagt tcaaacactg aaaccacttg
     5401 cttaatctca attgcccgtc agcttcgtct caaattgcgt tgtaattctt gtgctgcttc
     5461 acggcgattg gattccgcct tgctggcttt caccccattc aatgccgacc attcagaatg
     5521 gctgcgggct cggcgcaatg ggccaggcag attgctgttg ggccaaatca catcagcggc
     5581 ctgtaaactc aaggtttgct gcgcggcatg ccctcgttgc agcaaagcca ggatcaaagc
     5641 ctgcgctcgg gtcgcaagca aggtaatggt agcgggatcg atgtgtatgg ttttgacgcc
     5701 actgagttta tcggacaaac tgcccatatt cgacaacacc ccccctatca caccacccaa
     5761 tgccatgccc atcccacccg acaaacccgc agtggcggca tccacaccca agcccagcaa
     5821 agcgcctact gcggcacctg tgctggtgcg caagccaaat tgcttcagca attcgggatc
     5881 aaaagggtct tggctaaagg ctttgggcat ccaatcggca ggcaaatcgg cttgctgata
     5941 aaaccgaaac gcttgtaaca aatcttgctg aaaatggcgt tcacgctgac gcactttatt
     6001 ctgcatttgc gcaagtacag gctctaccgc gtcttcgtct ttgatggttt ccacaaatgc
     6061 cgccacctcg agcaaaaagt ttgccatcag tgcccaagcc gtgtcttgca aggcttgcca
     6121 ttccaaagtg cgcacagttt gcaattgctc taacacaggc ttgcggttca gcaaaatgtc
     6181 caattgcttc cacaaacgca tctcaccatc aaaatcaaat gccacggtgt caaactggct
     6241 gtgcacatgc aagccgcgcc gtgccaacat atcatgccat gcactggcat caccttgctc
     6301 ggtgaaattg aacacaggca tcacaggctt ggcgcaccaa gacaacaccg tcaattcgtc
     6361 tttatacttt gccaatactg gctcacgcgc gtctatcaca tacaggctga catcgctggc
     6421 caacacctgt cgcaacacct tggcctcttg gctgaactcg tgttgcgcca catcggcctg
     6481 taacaattgc tgcaaacgct caatgccatc actgcgactg gaagtgtgtt gttccagcca
     6541 atccagtacc ccgcccgcgt cttcaaggcc aggtgtatca aacaacttca ccaaagtctg
     6601 accttggtgg gtgatttctg ctacctcgac atggcgcgtg gtggcagggg catttttgac
     6661 ttctccaaac gcctcatcac gcagcaaggt acgcaacagc gaggttttac ccgtattggt
     6721 gtgcccaacc accgcgatgt ttaaggtttc attcaccatc gttactatcc atccattgcc
     6781 attctatggc ctgctcattc aatgcttgct gccacaaacg caaacgttca ccatcgctgc
     6841 cttcgcccca caagcacacc agcatgccgc cttgcgccgc atcagcataa gcgtcgaagc
     6901 ggcgcatact gcccctgtca ggcaagcctt gcaagcgcac tgccaatacc aaacgtgcag
     6961 gagcggcctg taagtgttgt aacaaagcct cttgctcagt gcgactgtct atcacccctt
     7021 ggtcttgagc atttacaggc cgcatcttcg tcaacaatgc ggcacagggc aaactttccc
     7081 acgcagcaaa cacagtttgg gccgcagtct gtactggacg ggtttgctca cgcttggcat
     7141 cggcacggta gtcattgctg ttgtcgacga tgtgggtttg ccataaacgc tttaagcgtt
     7201 ggtaatacgg caagttcaag ggcaaactgg ggctgtaagc ccgcaaacgc caacggcacc
     7261 acagccatgc caacaaacgt ggcagcaagc catacaccaa caaagacgac cacagcaaat
     7321 tcgcccattg ccgtgaaaag gccacctcac ccacgctttg gctggcggcc accgtgtgtg
     7381 catcgggcac attcgagccc aacacactgg gcaaccatgc caaagccgcc accaccacat
     7441 tcaaactgcc ttggtctagc agcgtggatt gccaactgaa agtgtattgg cgcaccaata
     7501 acatcaccac cacccccaat aacaaaccac ccaaactcgc caaccacatg ccgtgactga
     7561 tacaacccac ccaccatttc atttgtggct gcgccaattg ttcgcgcaac aacgcaaaca
     7621 aggcctgatg ctcaggtttg cgctgcgccc ctagccccaa accggcctgt aaccatgcgg
     7681 gaacatgggt gttgggcttc aacagatacc acaaccaagc cacaaacatc agcgcgttca
     7741 atcccaacac gctgaccaaa acataaaaga aattcaatga cggggtttgc atcaaaccca
     7801 cagcggtaaa aatacccaat accaaccaca ttacccacaa gatttggcgc aacagcaccg
     7861 cacgacccgt gtattgctgc aacattgtgt gcacatgctg gtcgctgtct atgcgctgcg
     7921 cccgcagcca cagcttgctg tcaaaatcgg catcggtgtg cttgagttcg gcattgatgc
     7981 gcgcagggtc actgggaaaa acaaaatgtt gttcttccaa gcgccgtatc aactcaatgt
     8041 atgcatgatg taaatgaatc acatgaacct tactcaaatc ggatgtcgca ttgtatcggg
     8101 agaaattggc aatagacaaa tgtttacagg ccgcttgatg cggcctgtaa gctgagcgca
     8161 atctttaaaa acggtaggaa gtgttcacaa acaccgaagt ttgcgcattc ggcgcgtctc
     8221 ccacttcagc caatacccca gcacccactt gccaatgctc gctgatattg ccgttcaccc
     8281 ccaactccaa cacaccactg tttttggcat cacccgtgtt aactggtaag acaaaatgca
     8341 tgtcgtccac gctgttcaaa cgcgcgccca ccacatcgtc cacatctttg aattcatgtt
     8401 gccactgggc cgagccatac acttgccacg catcgttcac cgcccattgc gcttgatagc
     8461 ccaagcccga ttggatggag gtgtgctctt gttcatcaaa actcatcgcg gtagacaact
     8521 tgccttgctc tcgcaacgac ttcaccgtgg ctttttgtgc actcaaagac aacactgggc
     8581 catggcttat cttgccttgc tgccaattca tgccagcgct gattttaccg ccatattggt
     8641 caccatcggc gctgccatgg tgtatgcgct cagctgctcc taattgcaca cggcggtggc
     8701 tgtccacatc caaattggca taatagactt gaccattgag ccacagattt tggtgttgcc
     8761 aacggccat
//
LOCUS       DAOMVK010000034         8732 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_2072_length_8732_cov_3.474588, whole genome shotgun sequence.
ACCESSION   DAOMVK010000034 DAOMVK010000000
VERSION     DAOMVK010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 8732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8732
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2072_length_8732_cov_3.474588"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..354)
                     /locus_tag="PK856_02185"
     CDS             complement(184..354)
                     /locus_tag="PK856_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01213.1"
                     /translation="MKWLCGIIACLFVLTACSSNKSGSISSHTGRERKAMRVHAVDAP
                     PPVLLAPSDSTE"
     gene            complement(367..1143)
                     /locus_tag="PK856_02190"
     CDS             complement(367..1143)
                     /locus_tag="PK856_02190"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRL01214.1"
                     /translation="MIDSHCHLLDEPLYQKLPEIIQAAFTSGVDRFIVPCSHANDFQT
                     LAKLTAEYSCILPAFGIHPYYVAETELHEALDQLQHCLATHPHALVGEIGLDFADKFL
                     SPVQRQQQHAYFEAQLQLAQQHQRPVILHHRQSLDAMLKTMQAQGFECAGFLHAYSGS
                     IEQARPWLQLGFKLGLGSVLLKPNARKVFQLLPHLKHSDYVLETDAPYMVSSSMNQAY
                     NEPKFVRAVAETLAEFYQCSWEEIATHSNENIASVLNPSR"
     gene            complement(1151..1567)
                     /locus_tag="PK856_02195"
     CDS             complement(1151..1567)
                     /locus_tag="PK856_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HRL01215.1"
                     /translation="MQNETSIGAALKAAVQTMSKKKQTDMIADHVYEKYDVFKKFKPL
                     AVGIDQDLIAALPQFEATLIARVLANHCRRPRYIKALARGGKRFDLNGRFKGEVTAEE
                     QQIAQNHPCMQTNKAETAEATSTEADSADVKETDNN"
     gene            complement(1712..2722)
                     /locus_tag="PK856_02200"
     CDS             complement(1712..2722)
                     /locus_tag="PK856_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01216.1"
                     /translation="MVKAEPIRVVKDSTPVVFAQATDTKSKRASSVFNLDHDAKTKAE
                     TSAFEASTKPAVARFTESTPTATLTDVALKESISATPDNNTWDRGDLTQDDMSIIAPA
                     TAAVITPAMAIAAKNVAASKDTPRSSTYANSPSSSRSSNSKAANTAKPNTNTVKAVES
                     SKSTTATRKKQEANKEASNKATNTKSINKTNAKQAAKGKQNNTQTEVENRKNSKTNSK
                     NNRNKTETGKNQRNNDKSAATETKSTSKNSRNKAENTNDTKASKGKNANERNTKSDNK
                     DSKTNSKKPVNKTSESTKNNKKATQEQDNNSKSKNNNSKTKTTDKKTDSAKKTPKKDK
                     AK"
     gene            2703..3035
                     /locus_tag="PK856_02205"
     CDS             2703..3035
                     /locus_tag="PK856_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01217.1"
                     /translation="MGSAFTNGVALVGTEAILGLLLVAKLFSGLFRAVKSTAFCDEVL
                     DLFLALVSALASVVVLPWPHLLGNCFHARDSKLPLSYSLISLLLPLFTTSMVMPSTLV
                     NGLIKPTL"
     gene            3491..>3568
                     /locus_tag="PK856_02210"
     CDS             3491..>3568
                     /locus_tag="PK856_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid decarboxylase"
                     /protein_id="HRL01218.1"
                     /translation="MTSHTTIQTITLGLTGASGMPYGIRL"
     assembly_gap    3569..3578
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3579..4116
                     /locus_tag="PK856_02215"
     CDS             <3579..4116
                     /locus_tag="PK856_02215"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HRL01219.1"
                     /translation="ECLLHLDKTVWLVYSQAAQIVAQQEIGLQLPSSASACEAMLHEK
                     YGVDDSRLRVFGQNQWSAPCASGTHVADAMVVCPATMGAVAAIAHGTSDHLLERAADV
                     SIKEGRPLIIVPREAPFSAIHLENLLKLARLGVCVLPPSPGFYQHPKTVDDIVDFVVA
                     RILDQLRIPHDLLPRWGE"
     gene            4441..4971
                     /locus_tag="PK856_02220"
     CDS             4441..4971
                     /locus_tag="PK856_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004286343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01220.1"
                     /translation="MTQLATCEPTETQTQTMQTLCLVVDGNSHASELLQDALPHHGML
                     VSEALLPQIRLVAMMIAASRLDFSHSTPEQITEEADWFAARMLVLGVNQFHLDVSLLP
                     MLHRANQRAAHFAKLHGLVFQEASMKMSLHAGRASNVLLMEVAAETLSTDLGLVANSL
                     ALKQQVSLQSGFCNLM"
     gene            4979..6169
                     /gene="dapC"
                     /locus_tag="PK856_02225"
     CDS             4979..6169
                     /gene="dapC"
                     /locus_tag="PK856_02225"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HRL01221.1"
                     /translation="MNSLMQQLNPYPFARLRALMQDINPPENVQPVMLHIGEPKHATP
                     QVIQDALVNNLSGLAKYPASKGIPELRAACSAWLKRRYQIDVDAETQILPVNGSREAL
                     FAFVQVVLDAQSDVKPVVISPNPFYQIYEGATLLAGGEVVYVNCNAPDFMPAWQDIEE
                     ATWQRTQLVFVCSPGNPSGAVMQLEDWQQLFALQDQYGFVIASDECYSEIYFGDDAPL
                     GSLQAASKLARSWDGLVMFTSLSKRSNVPGLRSGFVAGDAKLMAAFLEYRTYHGSAMG
                     LPIQHASIAAWNDEEHVRLNQALYQQKFDLFVSKLQAAYKISIPDASFYVWLKVPNGD
                     DVAFTQRLWQTQGVQVLPGRLLARDTPQGNPGQGYVRIALVATVEECEQAAERLLTFA
                     QRGF"
     gene            complement(6144..>6834)
                     /locus_tag="PK856_02230"
     CDS             complement(6144..>6834)
                     /locus_tag="PK856_02230"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HRL01222.1"
                     /translation="PRAEDLLQQMHQLDAYAGSIKNEYHLQADIGIIQMLPPSLLAEC
                     VGLIKEHFPHIHQTFSLGLSGIILNQVRRKELQGGFFVGDNPYRNLHVIDLLPLQFEV
                     VMPSDWQNQFANSSEAMQNLPWISLSAFSALTKFEQKIWRQWKVKPTTALQCDDMYAL
                     LALVEQKHGLALLPSHYLDQHPNALIHRLPDIQLSEMLSFIYPIELELDPVITAWRTI
                     LEQVKNHAVQM"
     assembly_gap    6835..6844
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7312..7689
                     /locus_tag="PK856_02235"
     CDS             7312..7689
                     /locus_tag="PK856_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HRL01223.1"
                     /translation="MENNIARQVLNNPKFQKMAKTKRMYCWSFSILMFVIYVIYILYI
                     GASPESFGVPVAAGKTTTVGIYVGLFVILFAISITGIYVRQANGPLEEITQEVVREVT
                     AANAPATHKPSVQSADSDTAKEA"
     gene            7691..>8732
                     /gene="actP"
                     /locus_tag="PK856_02240"
     CDS             7691..>8732
                     /gene="actP"
                     /locus_tag="PK856_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283355.1"
                     /note="member of the sodium:solute symporter family;
                     cotranscribed with the acs gene which encodes acetyl
                     coenzyme A synthase; mutations affect acetate uptake;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HRL01224.1"
                     /translation="MKKLLLTSLAMAASTLVMADALTGDVEKQAVNIPAIVMFFIFVA
                     GTLYITFWAAKQNKSTKDFYTAGGGISGFKNGLAIAGDYMSAASFLGISAMVYTTGYD
                     GLIYSTGFLVGWPIVLFLIAERLRNLGKFTFADVAAYRLKQKPVRMFAASGTLLVVII
                     YLIAQMVGAGKLIELLFGLDYMYAVIIVGVLMVLYVLFGGMLATTWVQIIKAVMLLSG
                     ATFMAIMVLKMADFSPEVMFTKAVAVHPKGDAIMSPGGLVKNPIDALSLGLALMFGTA
                     GLPHILMRFFTVKDAKQARKSVFVATGFIGYFYILTFIIGFGAIMMLTNNPNFFHEVM
                     KDGKPVMELIGGT"
BASE COUNT         2021 a         1865 c         2213 g         2613 t
ORIGIN      
        1 cgctcacgtg gccgccgatt tgctgtgttt gatgcgcaac cgcaaattgg caggaaagtt
       61 gtaacttgta atacgccgat gcataagtgg cctgtaacat ggctgatgta gagggtagca
      121 tcggtgatat gcacaaaaaa gcggcctgta atctgtttac aggccgcttt tgatatggct
      181 gggttattca gtgctgtcac tgggtgcaag caatacggga ggaggggcat ctacggcatg
      241 tacacgcatg gctttgcgtt cacgacccgt gtggctactg atgctgccac ttttattgga
      301 gctgcatgcg gttaacacga acagacaagc gatgatgcca cacaaccatt tcatattgat
      361 ttcctctcag cgactggggt ttaacacgct ggcaatgttt tcattgctgt gagtggcaat
      421 ctcttcccag ctacattggt aaaactccgc caatgtttct gccacagcgc gcacaaattt
      481 tggctcatta tatgcctgat tcatgctgga cgataccata tatggggcat cggtttccag
      541 tacataatcg ctgtgtttta aatggggcag taattggaaa accttgcgtg cattgggttt
      601 gagtaaaacc gaacccaagc ccaatttgaa tcccagctgt agccatggtc tggcttgttc
      661 aatgctgccg ctgtaagcat gtaaaaatcc agcacattca aaaccttgtg cttgcatggt
      721 ttttaacatg gcatccaaag attggcggtg atgtaaaatc acagggcgtt gatgctgttg
      781 tgcaagctgc agttgtgcct cgaaataagc gtgttgttgc tgtcgttgca ctgggctcag
      841 gaatttgtct gcaaagtcca agcctatctc gccaaccaaa gcatgcgggt gtgtagctag
      901 gcaatgttgc aactgatcca aagcctcatg cagctccgtt tctgcaacat aataaggatg
      961 gatgccaaat gcaggcaaga tacagctgta ttcagcagtc aatttggcta aggtctgaaa
     1021 atcgttcgca tggctgcatg gcacaataaa tcgatcaaca ccagacgtaa aagctgcctg
     1081 aatgatttca ggcagctttt ggtacaaagg ttcatcgagt aaatgacaat ggctgtcaat
     1141 caatcgtgtt tcagttgtta tcggtttctt tgacatcggc actgtcagct tctgtcgaag
     1201 tggcttctgc agtttcagct ttgttggttt gcatgcaagg gtgattttgc gcgatttgct
     1261 gctcttccgc tgttacctca cctttgaagc ggccattcaa atcaaaacgt ttaccgccac
     1321 gagccaatgc tttgatgtag cgtgggcggc ggcaatggtt ggccaagaca cgtgcaatca
     1381 atgtggcttc aaactgtggc aatgcagcaa tcaaatcttg atcaatgcca acagccaaag
     1441 gtttgaattt tttgaataca tcatatttct cataaacatg atcggcaatc atgtctgttt
     1501 gttttttctt gctcattgtt tgcacagcgg ctttcaaagc ggcgcctatg ctggtttcat
     1561 tttgcatgtt tgcttcagtc cttaccacat caagacaacg acggcagcat aagtgccgtc
     1621 tgacctagga gcatcttaac cgattttaac cgcgggagca aaaaaactgt acacaagatg
     1681 cgattggaat atcggttgag agtatgaatg cttatttggc cttgtctttt ttaggtgttt
     1741 ttttcgcaga atcagttttc ttgtcagtgg tttttgtttt gctgttattg ttttttgatt
     1801 tactgttgtt atcctgttct tgagtggctt ttttgttgtt ttttgtgctt tcgctggttt
     1861 tgtttactgg ttttttactg ttggttttgc tgtctttatt gtctgatttt gtgttgcgct
     1921 cattggcatt tttgccttta ctggctttag tatcattggt attttctgct ttgttacggc
     1981 tgtttttact ggtgcttttt gtttcagtcg cagcagattt atcgttgtta cgctggttct
     2041 tgccagtttc ggttttgtta cgattgtttt tactgttggt tttgctgttt ttgcgatttt
     2101 ctacttcagt ttgggtattg ttttgtttgc ccttagcagc ttgtttagca ttggttttgt
     2161 tgatggattt tgtgtttgtg gctttattgc ttgcttcttt attggcttct tgttttttac
     2221 gagttgctgt ggtggattta ctcgattcca cagctttgac cgtgttggtg ttgggtttgg
     2281 ccgtgttagc cgctttgcta ttgctgctgc gacttgagct tgggctgttt gcataagtgc
     2341 ttgaacgcgg cgtgtctttg cttgctgcca cattctttgc cgcaatcgcc atggctggag
     2401 tgatgactgc cgcggtagca ggggcaataa tgctcatgtc gtcttgagtc aaatcgcctc
     2461 ggtcccaagt gttgttgtct ggtgtggctg agatgctttc tttcagagcc acatcagtaa
     2521 gtgttgcagt tggggtgctt tctgtaaatc tggcaactgc aggcttggta cttgcttcaa
     2581 aagctgaagt ttcagctttg gtttttgcgt catggtcaag gttgaatacc gatgatgcac
     2641 gcttggattt tgtatcagta gcttgtgcaa agacgactgg ggtgctgtct tttacgacgc
     2701 ggatgggttc ggctttcacc aatggcgtgg ctttagtggg cactgaggca atattgggtt
     2761 tgctgctagt agcgaaactg ttttcaggtt tgttcagggc agtaaaatcg acagcatttt
     2821 gtgatgaggt gcttgatttg tttttagcac ttgtttctgc tttggcaagc gtggtggttt
     2881 tgccttggcc acatttgctg ggcaattgct tccatgcacg cgacagcaaa ttgccattgt
     2941 cgtatagctt gatttcgctg ctgctgcctt tgttcaccac atccatggtg atgccttcaa
     3001 ctttggtaaa tgggttaatc aagcctacac tgtgattgtt gtcggtatag actttgtgcg
     3061 acacaaattc aggaggaagt ttgaattcac tgcttaagtg ttgttgtaag cacaaggcgg
     3121 tgttgttggc atcttgagtt gaaaccgtgg agctgaatgc tgcgctgctg cgcttaggca
     3181 tcaactggta atccagtgca tccactgaac ctatagaggt gcgttgcttc agattgggat
     3241 ttttgtctac cacaaacgtc gacgtaggcg cagcacaagc agcaagagac gctaacacag
     3301 cgcaaaccaa gaggcgacgt acaaacataa tgtatcccaa gcagatgaag tatggaacat
     3361 tgtaaccaat tacactgctt aggttcacgg aaaaaatgca aaattaatta aagtcactgt
     3421 gcattcggtt tgaatttgca ttaaaaccgc cattacaatg tcaccattcc cgtttacagg
     3481 ccgcttatta atgacgtctc atacaaccat acaaaccatc acccttggac taaccggcgc
     3541 atccggtatg ccttatggta tccgcttgnn nnnnnnnngg agtgcttgct gcatttggac
     3601 aaaacggtgt ggctagtgta ttcgcaagca gcgcaaatcg ttgcgcagca agaaataggc
     3661 ttgcaactgc catcgtcagc cagtgcttgt gaggcgatgc tgcatgaaaa atatggggtt
     3721 gatgacagcc gtttgcgtgt gttcgggcaa aatcagtggt cggctccgtg tgcatcaggc
     3781 acacatgttg ccgatgcgat ggtggtgtgt cctgcgacca tgggcgctgt ggctgcgata
     3841 gctcatggaa cttctgatca tttgttggag cgcgccgccg atgtgagcat caaagaaggg
     3901 cggcccttaa tcatagtgcc gcgtgaggca cctttttcag ccatacattt ggaaaacctg
     3961 ctcaaactcg cgagattggg cgtgtgtgtg ttgccaccgt cgccaggttt ttatcaacac
     4021 cccaaaaccg tggatgacat tgtggatttt gtggtggcac gtatcttaga ccagttgcgc
     4081 attccgcacg atttgttgcc acgttggggt gagtagtgga gggtttgcgg caattattgg
     4141 aattgctgtg gcgttttgtg tcatggatta tgtggctttt ggaaaaatta gctggctagg
     4201 tttgacaatt tttaaaaata ccgttagtgt ggcaggcatg aataccttaa tgacgcaatc
     4261 tttctctttt tcttactatt ggtaccgctc aaatccagcg gcgcttagtc gtgtttaaga
     4321 gaaacacgca gtcataagca aataagccgc tcagttgagg cggctttttt tgtacccacc
     4381 atcgtctcaa ctggaagtgt tgcaagccac tacgcaggaa aaccatacag gagacgagcc
     4441 atgacccaat tagcaacatg tgaacccact gaaacacaga cccaaaccat gcagactttg
     4501 tgtttggtgg tggatggtaa tagtcatgcc agtgaattgt tacaagacgc tttgcctcat
     4561 cacggcatgt tggtcagtga agccttgttg ccacaaattc gcttggtggc aatgatgatt
     4621 gcggcatcgc gtttggattt cagtcacagc acacctgagc aaatcaccga agaggctgat
     4681 tggtttgcgg cccgcatgtt ggtgttgggt gtcaaccaat ttcatttgga tgtgtctttg
     4741 ctgcccatgc tgcaccgtgc caatcagcgc gcggcacact ttgccaagtt gcatggtttg
     4801 gtgttccaag aggcgagcat gaaaatgagt ttgcatgcag gtcgcgccag caatgtgttg
     4861 ttgatggaag tggctgcgga gaccttaagc actgacttgg gtttggtggc aaatagtttg
     4921 gcgcttaagc aacaagtttc gttacaatcg ggtttttgca acttgatgta agcgactcat
     4981 gaattcatta atgcagcaac tcaatccgta tccgtttgca cgtttgcgtg ctttgatgca
     5041 agacatcaac cctcctgaaa acgtgcaacc agtgatgttg cacatcggtg agcccaagca
     5101 tgcaacgccg caagtcatcc aagatgcttt ggtgaacaac ctgtctggtt tggccaaata
     5161 cccagccagc aaaggcatcc ctgaattaag agcggcctgt agtgcttggc tcaaacgccg
     5221 ttaccaaatt gatgtggacg cggaaacgca aatcttgcct gtcaatggca gtcgcgaagc
     5281 cttgtttgcg tttgtgcaag tggtgttaga tgcgcaaagc gatgtcaaac ccgtggtcat
     5341 cagcccgaat ccgttttacc aaatttacga aggtgcgacc ttgcttgcag gcggcgaggt
     5401 ggtgtacgtc aattgcaatg cccccgattt catgcccgct tggcaagaca ttgaagaagc
     5461 cacatggcag cgcacccaat tggtgtttgt ttgcagccct ggcaacccat cgggtgcagt
     5521 gatgcagctt gaagattggc aacaattgtt tgccttgcaa gaccagtatg gttttgtgat
     5581 tgcgtctgac gaatgttatt ccgagattta ttttggcgat gatgcgcctt tgggcagttt
     5641 acaggccgca tccaaactgg ctcgttcatg ggatggcttg gtgatgttca ccagcttgtc
     5701 taagcgttct aatgtgccag gtttgcgttc aggctttgtg gcaggggatg ccaaactgat
     5761 ggctgcattc ttggaatacc gtacctacca tggcagcgcc atgggtttac cgatacaaca
     5821 cgccagcatc gctgcatgga acgatgaaga acatgtgcgc ctgaatcagg ctttgtacca
     5881 acaaaaattt gatttgtttg tgagcaagtt acaggccgca tacaaaatca gcatacccga
     5941 tgcctcattc tatgtgtggc taaaagtacc caatggtgac gatgtggcct ttacccagcg
     6001 tttgtggcaa acacaaggtg tgcaagtatt gcctggtcga ttgttggcac gagacacgcc
     6061 tcaaggcaat cctggtcaag gttatgtgcg cattgccttg gtagcgacag tggaagagtg
     6121 cgaacaagca gccgaacgct tgctcacatt tgcacagcgt ggtttttaac ctgctctaag
     6181 atggtgcgcc atgcagttat cactgggtcg agttccaatt caatcggata gataaacgac
     6241 agcatttcgc tcaattgaat gtcgggcagg cggtgaatga gagcattggg gtgttggtcc
     6301 agataatggc tgggcagcaa ggccaaaccg tgtttttgtt ctaccaaggc caataaagcg
     6361 tacatgtcat cgcattgcaa ggcggtagtg ggttttactt tccactgccg ccaaattttt
     6421 tgttcaaact tggtcaaagc cgaaaaagcc gacagcgaaa tccatggcag gttttgcatc
     6481 gcctccgagc tgttggcaaa ctgattttgc caatcactgg gcatcaccac ttcaaattgc
     6541 aacggcaaca aatcaatcac atgcaagttg cggtaggggt tgtcacccac aaaaaagccc
     6601 ccttgcagtt ctttgcgcct gacttgattc aatataatcc cactcaagcc caagctaaag
     6661 gtttggtgta tgtgtgggaa atgctcttta attaagccca cacattcggc cagcaaactc
     6721 ggtggtagca tttgtatgat gccgatatcg gcctgtaaat ggtattcgtt tttgatgctg
     6781 cctgcgtagg catcgagctg gtgcatttgt tggagtaagt cctcggcgcg aggcnnnnnn
     6841 nnnnaaggat tgtcccgccg cggtgaggct catgccttgc gcattgcggt caaataactg
     6901 cacatccaag tgtttttcta aggctttgat ttgggcagac acagcgggtt gggacaaatg
     6961 caaggtttca gcagcttggg tgagattctg ctggtgggcc actgcaacaa aacttttcaa
     7021 ctgatttaaa tccaacgtct tctccgctgt agatttctgt tttttattaa taaaactttt
     7081 ttaggttttg cacaagtttt tcaacaggaa aatttttttt gtttgagtta acgcaaaaaa
     7141 cctgtgcgcc tgaaaatgtc gaaaatcaat ctaaataatt gtttttaatg aaatgttgcg
     7201 gcgcatcatc agtaatatta atccaagcaa tccaaaaaaa aaattatgct tcaccgattt
     7261 ctgcctttac attttctcac gtgcgcaagt caacgtacaa aggagagata agtggaaaac
     7321 aacattgcac gacaagtgct gaacaatccc aagtttcaaa aaatggccaa aacgaaaaga
     7381 atgtattgct ggagcttttc gattttgatg tttgtcatct acgtgattta cattttgtac
     7441 ataggagcga gtcctgaatc ttttggtgtg cccgttgcag caggtaagac caccacagtc
     7501 ggtatttatg tgggtttgtt tgtgattttg tttgccatca gcatcactgg catctatgtg
     7561 cgccaagcca atggcccact ggaagaaatc acccaagaag tggtgcgtga agtgactgcg
     7621 gccaatgccc cagcaacaca caaaccgtct gttcaatctg cggacagcga tactgccaag
     7681 gaggcgtaga atgaaaaaac tcttgctcac aagtttggcc atggcggcca gtaccttggt
     7741 catggctgat gctttaactg gggatgtgga aaaacaagcg gtgaacattc ccgccattgt
     7801 gatgttcttc atttttgtgg ccggtacttt gtacatcact ttttgggctg ccaaacaaaa
     7861 caaatccacc aaagacttct ataccgcagg cggcggcatt tcaggcttta aaaatggctt
     7921 ggcgattgcc ggtgactaca tgtcagccgc gtctttcttg ggcatttccg ccatggtgta
     7981 taccacaggc tacgacggct tgatttactc tactggtttc ttggtgggtt ggcctattgt
     8041 gttgtttttg attgcagagc gtttgcgcaa cttgggcaaa tttacctttg ccgacgtggc
     8101 cgcttaccgt ttgaaacaaa aacctgtgcg catgtttgct gcttcaggca ctttgttggt
     8161 ggtgatcatt tacctgattg cgcaaatggt gggagcaggc aagctgattg aattgttgtt
     8221 tggcttggat tacatgtatg ccgtgattat tgtgggtgtg ttgatggttt tgtatgtgtt
     8281 gtttggcggt atgttggcca ccacatgggt acaaatcatc aaagcagtga tgttgctctc
     8341 tggtgcgact ttcatggcga ttatggtttt gaaaatggcc gacttcagtc ctgaagtgat
     8401 gttcaccaaa gcagtggcag tgcacccgaa aggcgatgcc atcatgtctc ctggcggctt
     8461 ggtgaaaaac ccgattgatg ccttgtcatt gggcttggct ttgatgttcg gtacggctgg
     8521 tttgccacac attttgatgc gcttctttac cgtaaaagac gcgaaacaag cacgcaaatc
     8581 ggtgtttgtg gccacaggtt tcattgggta cttctacatc ttgaccttta tcatcggttt
     8641 tggcgccatc atgatgttga ccaacaatcc taacttcttc catgaagtga tgaaagacgg
     8701 caagcctgtg atggaattaa tcggtggtac ca
//
LOCUS       DAOMVK010000035         8595 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_2123_length_8595_cov_3.467330, whole genome shotgun sequence.
ACCESSION   DAOMVK010000035 DAOMVK010000000
VERSION     DAOMVK010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 8595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8595
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2123_length_8595_cov_3.467330"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..1006
                     /locus_tag="PK856_02245"
     CDS             104..1006
                     /locus_tag="PK856_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HRL01225.1"
                     /translation="MWFKQISFFVLPQDIDLNLEEAQTKLEDASFTPCQGLDWKSEGF
                     ARAVPFREDKVFNAQDSWRISLRQEDKVLPASVIREVLDEKIAEIQATDGRNVGRKEK
                     MELKEQITDDLLPRALTRSRYTEAIIDVPGKLLLVNQSNSNKAENFVSQLRQALGSFP
                     ATLPRTAESPTSLMTAWLEQSEAAGNFELDSDCELKGVGDAAPVIKISKQDLAADEVK
                     QHLEHGKVCTQLGLIWNEQIRFVLNEDMSLKRIQYLDMLQEEAANQGDDMESLMTATQ
                     IIMTQNLSLLIQELIDHLGGLIQA"
     gene            1106..1321
                     /locus_tag="PK856_02250"
     CDS             1106..1321
                     /locus_tag="PK856_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01226.1"
                     /translation="MSSLSGCIGYGYQQAVITPEQREQMAYDSQRVQDAERSERAERR
                     AERHEERMSQAEAYRRATSGRNVYILH"
     gene            1372..1698
                     /locus_tag="PK856_02255"
     CDS             1372..1698
                     /locus_tag="PK856_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01227.1"
                     /translation="MKNLLIVLTASAVLLTGCATGGSVGDMAIGDDSTAIKAGRNRAE
                     AQLSRAELDQHRRQRTNVSEELDLEQKKRSNKQQQIGGAMGTIIGGAATVGAVAGTVK
                     LLGSWF"
     gene            complement(1737..2636)
                     /gene="rarD"
                     /locus_tag="PK856_02260"
     CDS             complement(1737..2636)
                     /gene="rarD"
                     /locus_tag="PK856_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003797562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HRL01228.1"
                     /translation="MSLSTPEQEKQRGLLYAVSCYVLWGLFPLYWYPLTQTAMPASQI
                     LAQRVIWSTVFALFLVIVLRQFGPILQAIRTPKLLGVFAISAFLLGSNWLIYLWAITN
                     SHVLDASLGYFMSPLFSILLGRLFFNERMTRLQAASIGIAVLGVLWLVFLYGSVPWIA
                     LGLTISFGFYSLVRKLAPLAALPGLALETLCMLPIAVLYLLWQSQQGSLFVDLSPLNW
                     GLLIGSGIVTSVPLLLFAAGAKRISMTEMGIIQYISPTAQFVTGLLLFHEAFNTQRFV
                     GYALVWLAVIVYVATSLKKSKSA"
     gene            complement(2700..3953)
                     /locus_tag="PK856_02265"
     CDS             complement(2700..3953)
                     /locus_tag="PK856_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HRL01229.1"
                     /translation="MPNDTNELLSMLNDVAAQSSAEQELPAASLNANISNAGPQQLQQ
                     AYLNLQKIMLYAAEKNASDIFIAPEFPPAVKINGKITPLSSKPYSAETTRDTVLSTMT
                     PKQIKDFQNELELNYAIKANSDVRFRVNAYHEQGRIGMVMRKINTEIATLDELGLPPI
                     LKDLSLEKRGLIIVVGATGSGKSTSMAGMVDHRNKLLAGHIITIEDPVEYVHRHHKSI
                     ITQREVGLDTLSWHAALKSAMREAPDVVIVGEVRDEESMSRAMELAQTGHLCMCTLHA
                     TNANQAIERVINFFDEARHAQIFMDLALNLKAIISQRLVVKKDGTGRTVAMDIMLNNP
                     AVQDLVFKGDISGIKDIMNRAQEEGMQTFDQHLLRLYAADEIDYEEALRNADSINDLR
                     LSIKLWEEGRDKKHVFDRVDNLNLI"
     gene            complement(3967..5007)
                     /locus_tag="PK856_02270"
     CDS             complement(3967..5007)
                     /locus_tag="PK856_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003791105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HRL01230.1"
                     /translation="MQITDLLAFSVKNKASDLHISTGLPPMIRVHGDVRRINLPAANQ
                     EQVFDMITDVMNDNQRKIFQQNLETDFSFELPNIARFRVNAFMSNRGPAAVFRTIPST
                     VLTLEQLNAPKVFEKISDTPRGLVLVTGPTGSGKSTTLAAMINYINENHPAHILTIED
                     PIEFVHQSKKALVNQRELHEHTHSFANALRSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLRAVISQTLLKTKDGSG
                     RVAAHEIMLSTPAVRNLIRENKIAQIGSVMQTGQQHGMQTLDQCLQNLVRRNQISIDM
                     ARSKASNPDLIN"
     gene            5150..5836
                     /locus_tag="PK856_02275"
     CDS             5150..5836
                     /locus_tag="PK856_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRL01231.1"
                     /translation="MSQTTLIERYQTVIAHIKQLSHQYRQDETAVQLIAVSKTFPSAD
                     IRTLYQQGQRHFGENYIQEWSQKTDELADLDIIWHIIGQVQSNKSRTVAEKEAWVHTI
                     DREKIAKRLSEQRPVDCLPLNVCIEVNTSGSADKHGVNAENVLALARQVSAMSQLRLR
                     GLMCVPSHPEDAKQLEQEFALMQQLFKALQADGLNVDTLSMGMSADMEQAIASGATLV
                     RIGTAIFGHR"
     gene            5882..6691
                     /gene="proC"
                     /locus_tag="PK856_02280"
     CDS             5882..6691
                     /gene="proC"
                     /locus_tag="PK856_02280"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009118814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRL01232.1"
                     /translation="MKKLYFLGGGNMCQAIVSGLVKQADFDITVIDNNADKRAHLEQT
                     YGVSTGTDLPELQADDVLMLAVKPQDMAAATASVKSNGALILSIAAGLDIATLSAMLG
                     NERIIRIMPNTPAQVGLGVAGLFAPQGLQAADKQIAEQIMQAVGQVHWLDDESRIHAI
                     TGISGSGPAYVFYLMEALAQAAQAQGFDEKTARELSLATFKGAVTLAEQSGLPFGQLQ
                     KNVTSKGGTTFAAISSFEASNIAAEIEAGVQACVARSNEMQSQLAQVKQNG"
     gene            6684..7247
                     /locus_tag="PK856_02285"
     CDS             6684..7247
                     /locus_tag="PK856_02285"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HRL01233.1"
                     /translation="MVDAIYMLALAFGIVCFARMLLQVGRLHIDHPLAQFCMKTTQWL
                     IKPLRRIVPPLGRFDMASVVAAVLVIYLAYILVAVWRFQGFTELGNMRFLAGAVFASL
                     LDALKAFSYALLLGLLIQSVLSWQQRPTHLLTVLQNIYRPLTKPFAWMRYRQFDFSAA
                     VLALILWWWLVSVLPYLQQNLNNWSLQ"
     gene            7460..7876
                     /gene="dksA"
                     /locus_tag="PK856_02290"
     CDS             7460..7876
                     /gene="dksA"
                     /locus_tag="PK856_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HRL01234.1"
                     /translation="MAKLTEKDIINWSGPEEDYMNEDHLAFFKELLTKLQEEIIDNAS
                     ATTNHLQEQVVTPDPADRATLEEEYALELRTRDRERKLLTKVQASLRQIEDGSYGFCK
                     DTGEPIGLKRLLARPTANLSVEAQERREQVKKQFAD"
     gene            8014..>8595
                     /locus_tag="PK856_02295"
     CDS             8014..>8595
                     /locus_tag="PK856_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRL01235.1"
                     /translation="MLSAVIIEDEVLAAERLRVLLQDCQVTLLHHFEHPQTALDWLSQ
                     HQVDVAFVDIGLPEINGLQLVERLNRSMGVPPKVIFTTAYEEHALKAFDLAAIDYLLK
                     PIKMTRLQAALERIEPHKNTDEFTAFKVMSRDRMVEIPWQKSRYLVAEEKSVILVTAD
                     GQTYDLPKTLIYWEELLSERVIRIHRNALVMRHG"
BASE COUNT         2355 a         1862 c         2100 g         2278 t
ORIGIN      
        1 cgttagctat ggtgaagtct tgccaggttt gattgaagat tgagttatta aacgatgaca
       61 tttcagccac cagattggtg gcttttttat taggagattt caaatgtggt tcaaacaaat
      121 tagctttttt gtcctacccc aagatattga tttgaatttg gaagaggccc aaaccaagct
      181 tgaagatgcc agtttcacac cttgccaagg cttagattgg aaaagcgaag gctttgctcg
      241 tgctgtgccg tttcgagaag ataaggtatt caatgcccaa gacagctggc gcatcagtct
      301 acgccaagaa gacaaggtgt tacctgccag tgtaatacgt gaggttttgg atgaaaaaat
      361 tgccgaaatc caagccactg acggccgcaa tgttgggcgc aaagagaaaa tggagttgaa
      421 ggagcaaatc acggatgact tgttaccacg tgctttgacc cgctcacgct atacagaagc
      481 catcattgat gtgcctggta aattgctttt ggttaaccaa agcaatagca ataaagctga
      541 aaactttgtc agccagttgc gccaagcttt gggtagtttc cctgctactt tgcctcgtac
      601 agctgaatca ccaacctcat tgatgactgc atggctggag caaagcgagg ctgctggtaa
      661 ttttgagtta gacagcgact gtgagctcaa aggcgtcggt gatgcagcgc cagtcatcaa
      721 aatcagcaaa caagacttgg ctgctgatga agtcaaacaa cacctagaac atggcaaagt
      781 gtgtactcaa ctgggtttaa tttggaatga gcaaatacgc tttgtattaa atgaagatat
      841 gagcttaaag cggattcaat atttagacat gctgcaagaa gaagctgcga atcaaggcga
      901 tgatatggaa agtttaatga cagccaccca aatcatcatg actcaaaatc tcagcctctt
      961 aatacaagag ttaatagatc atttaggtgg ccttatacag gcataaaagt tacagcattt
     1021 ggttcaaaag cgaatgcttt ttgcagtaag atttggttta aaagcgagcg ccgattagtt
     1081 caaaagcgct cgccgcctta caatcatgag cagcttgtct ggctgtattg gctatggtta
     1141 ccaacaagca gtgattacgc ctgagcagcg tgaacaaatg gcttatgatt cacaacgtgt
     1201 acaagatgcg gagcgttcag aacgcgctga acgccgtgct gaacgtcatg aagagcgcat
     1261 gagccaagcg gaagcgtacc gtcgtgcgac cagcggccga aatgtttata ttttgcattg
     1321 atgtgacata tcaacaacca ttgatgattc atttatttta aggacttaat catgaaaaat
     1381 ttgctgattg tattgacggc atctgcggta ttgttaacag gttgcgcgac tggcggttca
     1441 gtgggtgaca tggccattgg tgatgactct actgccatta aagcaggccg taaccgtgct
     1501 gaagcacaat tgtcgcgtgc tgaattagac caacaccgtc gtcaacgcac gaacgtgtct
     1561 gaagagttgg atttggaaca gaaaaaacgt tctaacaaac aacaacaaat cggtggtgca
     1621 atgggtacga tcattggcgg tgcagccact gtgggcgctg ttgcaggaac ggttaaactc
     1681 ttgggttctt ggttctaaga gacccaacag aaaaaagctg ccttgggcag ctttttttat
     1741 gcagatttgc tttttttcag tgatgtcgcc acatacacaa tgacggcgag ccagaccaag
     1801 gcataaccga caaaacgttg ggtattaaaa gcttcatgaa acaacagcaa gccagtgaca
     1861 aattgagcgg tgggagaaat gtattgaatg atgcccattt ccgtcatgga aatgcgtttc
     1921 gcacctgcag caaacagcag taacggcaca gaagtcacaa tgcctgagcc gatgagcaat
     1981 ccccagttga gtgggcttaa atccacgaac aaactgcctt gttggctttg ccaaagcaaa
     2041 tacagtactg caatgggcaa catgcacaag gtttctaacg ccaagcctgg caaggctgct
     2101 aaaggcgcta atttgcgaac taagctgtaa aaaccgaaac tgatggttaa gcccaaagca
     2161 atccaaggta cgctgccata taaaaacacc aaccacaaca cgcccaatac cgcgatacca
     2221 atagatgcgg cctgcaaacg ggtcatgcgt tcattgaaaa aaagccgacc taataagatg
     2281 ctgaacaaag gcgacataaa atagcccaag ctcgcatcca aaacatggct gttggtgatg
     2341 gcccacaggt aaatcaacca gtttgaaccg agtaaaaagg ctgaaatcgc aaacacgccc
     2401 aataatttgg gagtgcgtat ggcctgtaaa ataggaccaa actgacgtaa tacgatgacc
     2461 aaaaacagag caaatacagt agaccaaatc acgcgttggg ccaatatttg gctggcaggc
     2521 atggctgttt gggtaagggg ataccaatac aagggaaata aaccccataa gacgtaacaa
     2581 ctgacggcgt ataacaagcc ccgttgtttt tcttgttcgg gggtggatag agacatttgg
     2641 gttcctcaga aaaatgaagc cacctgaggg tggcttgttt gaaggtaggt aactcgattc
     2701 taaatcaaat tcaaattgtc cacacggtca aatacatgtt tcttatcacg gccttcttcc
     2761 cacaatttga tagacaagcg caaatcattg atggagtcag cattgcgtaa ggcttcttca
     2821 taatcgattt catctgcggc atacaagcgt aacaagtgct ggtcaaaggt ttgcatgcct
     2881 tcttcttggg cacggttcat gatgtctttg atgccgctga tgtcaccttt gaacactaag
     2941 tcttgtaccg cagggttgtt cagcatgatg tccatggcaa cagtgcggcc tgtaccgtct
     3001 tttttcacca ccaagcgttg cgaaataatg gcttttaagt tcaaggccaa gtccatgaaa
     3061 atttgggcat ggcgggcttc gtcaaagaag ttaatcacac gttcaatggc ttggttggca
     3121 ttggtcgcgt gcaaagtaca catacacaag tgacccgttt gcgccaattc catcgcacgg
     3181 ctcatgcttt cttcatcacg cacctcaccc acaatcacca catcgggcgc ttcacgcatg
     3241 gcacttttca gcgcggcatg ccagctcaat gtgtccaaac ccacttcgcg ttgggtaatg
     3301 atgcttttat gatggcggtg cacatactcg actgggtcct caatggtgat gatgtgaccc
     3361 gccaataatt tgttacggtg gtctaccatg cctgccatac tggtggattt acccgaacca
     3421 gtggccccca ccacaatgat caaaccgcgt ttttccaacg acaaatcttt cagtatgggt
     3481 ggtaaaccca attcatccaa ggtagcgatt tcggtattga ttttacgcat taccatgcct
     3541 atacggcctt gttcatggta ggcgttcaca cggaaacgca catcagaatt ggccttaatg
     3601 gcatagttca attccaattc attctgaaaa tctttgattt gcttgggtgt cattgttgac
     3661 agcacagtat cccgcgtggt ttcagctgaa tagggcttgg aagacaacgg cgtgattttg
     3721 ccgttgattt tgacggcagg aggaaattca ggtgcgatga aaatatctga agcgtttttt
     3781 tctgccgcat acaacatgat tttttgcaaa ttcaaatagg cttgttgcaa ttgctgtggg
     3841 cctgcattgg aaatgtttgc attcagtgat gcggccggta gctcctgctc ggcacttgat
     3901 tgggcagcaa catcattgag catggataag agttcgttgg tatcgttagg cataatcgtc
     3961 actcaatcag ttaattaaat cagggttaga tgctttgctg cgcgccatgt ctatagagat
     4021 ttggttacgg cgtaccaaat tttgcaaaca ctgatctaag gtttgcatgc cgtgttgttg
     4081 gccggtttgc atcacagagc caatttgcgc aattttgttt tcacgaatca ggttacgcac
     4141 ggccggtgta ctcaacatga tttcatgtgc cgccacacgg cctgaaccat ctttggtttt
     4201 gagcaaggtt tgtgaaatca ccgcacgcaa actctctgac aacattgagc gcaccatctc
     4261 tttttcacct gcagggaaca cgtccacaat acggtcaacg gttttggctg cacctgtggt
     4321 atgcaaagta ccaaatacca agtgacctgt ttcggctgcg gtcaaggcca aaccgatggt
     4381 ttcaggatca cgcatctcac ccaccagaat cacgtctggg tcttcacgca aagccgatct
     4441 taaggcattg gcaaaactgt gggtgtgttc gtgcaattca cgctgattga ccaaggcttt
     4501 tttggattga tgcacaaact caatcgggtc ttcaatggtt aatatgtgag caggatggtt
     4561 ttcattgatg tagttaatca tcgcagcaag cgtggttgat ttaccagagc ccgttgggcc
     4621 tgtcaccaat accaagccgc gaggggtatc ggaaattttt tcaaatactt tgggtgcatt
     4681 caattgttcc agcgttaata ccgtgctggg aatggtacga aacacggcag ctgggccacg
     4741 gttcgacatg aaagcattga cacgaaaacg ggcaatattc ggcaattcaa acgaaaagtc
     4801 ggtttctaaa ttttgttgga agattttgcg ttggttgtca ttcatcacat cggtaatcat
     4861 gtcgaacact tgttcttgat tggccgctgg caagttgatg cgacgcacat caccatgtac
     4921 ccgtatcatc ggtggcaagc ccgtgctgat gtgtaagtct gatgccttgt ttttaacgct
     4981 gaacgctaat aaatcagtaa tttgcatgtt tttcccttaa gcgcacaaac ctcattttag
     5041 ccttatggat gctgcttctc agccgtgctg acaggctttg attgtgttaa ggttagaagt
     5101 tgtttgtgtt gttgttgtca gtccatccgt tacatcgtgg agccgtctta tgtctcaaac
     5161 caccttaata gaacgatatc aaacggttat cgcccacatt aagcaattat cacaccaata
     5221 ccgtcaagat gaaacagctg tgcagctgat tgctgtcagt aaaacgtttc caagtgctga
     5281 tattcgcacg ctttatcagc aaggacaacg tcattttgga gaaaattaca tccaagaatg
     5341 gtcacaaaaa accgatgaat tagccgattt ggacatcatc tggcacatca ttggccaagt
     5401 ccaatcgaat aaaagtcgta cagtggcgga aaaagaggct tgggtacata ccatagaccg
     5461 tgaaaaaatt gccaagcgtt tgagcgaaca gcggcctgta gattgcctgc ctttgaatgt
     5521 ttgcattgaa gtgaatactt caggcagtgc ggataaacat ggagtgaatg ctgaaaatgt
     5581 gttggcattg gcgcggcaag tgagtgcaat gtctcaattg cgtttgcgtg gtttgatgtg
     5641 tgtgcccagt catccagaag atgccaaaca attagaacaa gaattcgcat taatgcaaca
     5701 attattcaaa gccttacagg ccgatggtct gaatgtggat actttgtcca tgggcatgag
     5761 tgccgatatg gagcaagcca tagcctctgg tgccacgctt gtgcgcatcg gcaccgccat
     5821 ctttggccat cgctaaacat tcaaaattca cagcatccaa atatcaagaa agaataaaaa
     5881 catgaaaaaa ttgtattttt taggtggagg caatatgtgc caagccatcg tctctggttt
     5941 ggttaaacaa gccgattttg acatcactgt gattgataac aatgcagaca aacgcgccca
     6001 tttggaacag acttatggcg tgagcacagg cacagatttg cctgagttac aggccgatga
     6061 tgtgttgatg ttggcggtaa aaccgcaaga catggctgct gccacagctt ctgtgaaaag
     6121 caacggggcg ctgattttat cgattgctgc aggcttggac attgccactt tgagcgcgat
     6181 gttgggcaat gaacgcatca tacgcatcat gcccaatacc cccgctcaag tgggtttggg
     6241 tgtggctggt ttgtttgcgc ctcaaggctt acaggccgct gataaacaaa tagccgaaca
     6301 aatcatgcag gctgtggggc aggtgcattg gttagatgat gaaagccgca tccatgccat
     6361 cactggcatc agtggcagtg ggcctgccta tgtgttttat ttgatggaag ccttggcgca
     6421 agccgctcaa gcgcagggat ttgatgaaaa gactgcacga gagctgagtt tggccacttt
     6481 caagggcgcg gtgacgctgg cagaacaatc gggcttgccc tttggccaat tacaaaaaaa
     6541 cgtgacttca aaaggcggga ccacatttgc agccatttcg tcttttgaag caagcaacat
     6601 tgctgcagag attgaagcgg gcgtgcaagc ctgtgtggcc cgatcaaatg aaatgcaaag
     6661 ccaattggcg caggtaaagc aaaatggttg atgccattta tatgctggcc ttggctttcg
     6721 gtatcgtgtg ctttgcccgc atgctattgc aagttggccg tttacacata gaccacccat
     6781 tggctcagtt ttgtatgaaa accacgcaat ggctcatcaa gcctttgcgg cggattgtgc
     6841 caccattggg gcgctttgac atggcgtctg tcgtggctgc ggtgttggtc atttatttgg
     6901 catacatatt ggtagcggta tggcgtttcc aaggctttac cgagttgggc aatatgcggt
     6961 ttttggcggg tgcggttttt gccagcttgc tggatgcact gaaagcattt agctatgcct
     7021 tgttattggg tttgctcata cagtcggtat tgagttggca gcaaagaccc acacatttgt
     7081 tgacggtttt gcaaaatatt tacaggccgc ttactaagcc atttgcctgg atgcgttacc
     7141 gccagtttga cttttcggct gcggtattgg cattgatttt gtggtggtgg ttggtgtcgg
     7201 tattgccata cttgcaacaa aacttaaaca attggtcttt acaataagcg gtttatgtgg
     7261 tttaaagtag ggcttcatta aacctatgtg cgattgatag gtcttaggct catgcgatca
     7321 gccttaactt taaaacagga acttagatta aatcggatgg cgtcaaagtg tgttactatg
     7381 ccgcgctatt ttttataaat caaaccgatt taactgactt gccgtttcgc gcggcgattg
     7441 ataagtggag gggctaatca tggctaaact aaccgaaaaa gacatcatca actggtcagg
     7501 accggaagaa gattacatga atgaagatca cttggcgttc ttcaaagaat tattgaccaa
     7561 attgcaagaa gaaatcattg ataacgccag tgccaccacc aatcatttgc aagagcaagt
     7621 ggtgacacct gatcctgccg accgtgccac gttggaagag gaatatgcgt tggaattgcg
     7681 cacccgtgac cgcgaacgca aattgttgac caaagttcaa gcttctttgc gccaaatcga
     7741 agacggttct tacggttttt gcaaagatac gggtgaacct ataggcttga aacgcttgtt
     7801 ggcacgccca acagccaatt tgtccgtaga ggcgcaagag cgtcgcgaac aagtgaagaa
     7861 acaatttgct gattgatatc aacagcatca aaagccgctg ccaaaatggg gagcggcttt
     7921 ttttattcgg ttgaattgag gcaattggca taatttgtgt taggttctta ttttgaatca
     7981 caaacacaac aacacattca aaagggaata agcatgttaa gcgcagtgat tattgaagat
     8041 gaggtgttgg cagccgagcg cttacgcgtt ttgttgcaag attgtcaggt gaccttgctt
     8101 caccattttg agcatccgca aacagcatta gactggctca gccagcacca agtggatgtg
     8161 gcatttgttg acataggctt gccagaaatc aatggtttgc aattggtaga gcgtttaaac
     8221 cgttctatgg gcgtgccacc caaggtgatt tttaccactg cttatgaaga acatgcgctc
     8281 aaagcctttg atttggctgc aatagactat ctgttaaaac ccatcaagat gacacgctta
     8341 caggccgctt tggagcgtat cgagccgcat aaaaacaccg atgaattcac ggcctttaaa
     8401 gtcatgagcc gtgatcgcat ggtggaaatt ccgtggcaaa aaagccgtta tttggtcgcc
     8461 gaagagaaga gtgtgatttt ggtcaccgcc gatggccaaa cctatgattt gcccaaaacg
     8521 ctgatttatt gggaagagct gttgtctgaa cgtgtcatcc gtattcaccg caatgccttg
     8581 gtgatgcgcc atggc
//
LOCUS       DAOMVK010000036         8325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_2203_length_8325_cov_2.897219, whole genome shotgun sequence.
ACCESSION   DAOMVK010000036 DAOMVK010000000
VERSION     DAOMVK010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 8325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8325
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2203_length_8325_cov_2.897219"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="PK856_02300"
     CDS             complement(<1..839)
                     /locus_tag="PK856_02300"
                     /inference="COORDINATES: protein motif:HMM:NF022518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HRL01236.1"
                     /translation="MAYIELVRRLEEQHHVFPSDPARINTELKHSDADFESKLWLRAQ
                     RIDSDQRVHGLLQQFLSRAVLLRQVLWVLWLVLGIFTAVGLMQTPALNFFYVLVSVLG
                     VNALMFVWWLVYLFKPQVHLPAWLQASMGLGAQRKAEHQALLALLREQLAQPQMKWWV
                     GSISHSMWLASLGGLLLGVVMMLLVRQYTFSWQSTLLDQGSLNTVVAVLAWLPSVLGL
                     NVPDAHTVAASQSVGEVAFSRQWANLLWSSLLVYGLLPRLLAWLCCRWCVQSYQPSLP
                     LALP"
     gene            complement(946..2721)
                     /locus_tag="PK856_02305"
     CDS             complement(946..2721)
                     /locus_tag="PK856_02305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HRL01237.1"
                     /translation="MRKTHLAVLLMVLGAGTVAEAGPFSQTYFLGDSLTDSGQFMGQR
                     FTTNPGKVWSQQLAEELGTAADPSGKKGTNYAVGGARVGVNSSTTVGGVTVPIPSVRT
                     QLQQLRAVEGNRLDGDALYTVWGGANDLFAVAAGAPAQTTITSAIIENVTVVSALSQA
                     GARYILVPNIPDLGMTPAFSGNVAAGTQLSTAYNQALYGQLADMKANVIPLDVASLLH
                     EAAANPSQFGLKNVTTPACGAVSSLQCGPGDLVEAHADQTYLFADGVHPTAAGHKVLA
                     DYATAVLTAPQQVVVLAPAANQAGIAQMQHIDRRLHNTAHIQDERFALWGQGTAVLGS
                     GDALGTELDGTGSAWMLGADKRMGNWTAGAYLAFDNLDGKLAKGSGYEEQRWAGGLYG
                     RWQHQNLWLNGQVYYANLDVDSHRRVQLGAAERIHHGSADGDQYGGKVSAGMNWQQGA
                     FTHGPVLSLSAQKATIKSLQEQGKQSTAMSFDEQEHISIQSGLGYQVQWAASNAWQVY
                     GSAQWQHEFKDVDDMVGARLNSVDNMHFELPVDTGDAKNSGVLELGVNGNITERWQVG
                     AGVLAEVGDAPNAQTSVFVNTSYRF"
     gene            2870..4171
                     /locus_tag="PK856_02310"
     CDS             2870..4171
                     /locus_tag="PK856_02310"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRL01238.1"
                     /translation="MNTPPLNIAIVGSGMAGLAAAYVLKQSGHHVTLYESQAKSGLDA
                     HSTTFAGGMVDAPLRVMNPQLWSHTLALAEHLGIQMYRVRTDMACSWMEGERIGATWF
                     SSRRQAHVGFPVLGSWRFAKQLPALALGVYQLKQALLHTEGLASTLSLAEWMQQYPIH
                     PLLWHGCVVPVLLTVCTCDVQTLLRWPARPLLVFVDLLLRDAGLYRLQGGTQALANAL
                     KQGVSLLNHTSVSAIHQNHAQIFVCDAAEHTQAFDHVIVATPTQACGFLDAEQFGLEK
                     QLLQQLPYSSGELVSHTDSSVMPRKRRDWSPLHYNMRRDFSAHSFSVWLNPIETSLSE
                     TAPAVFQSWNSLSPIADKTIIQQTCLTRAVCTPTSLQVVKQLQGLQADVQLNQRRLWL
                     CGSWSCEGLPILESAVTSALAVCKQLGAVLPVDLPSTSPLA"
     gene            4168..4965
                     /locus_tag="PK856_02315"
     CDS             4168..4965
                     /locus_tag="PK856_02315"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRL01239.1"
                     /translation="MSSKRRERINHSILGDARKQAWSNLGLWRTGQSDYRTAAQALAL
                     QLAEQMSLQAASSLLDVACGYGASLNVWHEMGIREITGLEPQTACVQAWQTDHRFQCW
                     HDSMQNISLLSQDQPFEAIIAIDAAYQWDVQLWLQQAHAALCPHGRLGFHFLLLADDF
                     AQRPLWQKLCTRALLRMVGVKSYLTQNQLQAALNAHFHQTWSEVSITDLSDEVLGGFG
                     HYALRLPTYPLDFDHLKIQATGHLCRYLHRQKSVRYVSICLQKTAPR"
     gene            complement(5000..5425)
                     /locus_tag="PK856_02320"
     CDS             complement(5000..5425)
                     /locus_tag="PK856_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01240.1"
                     /translation="MRLKHVALLGLLCMSANTWALMSKKCDEPKHKEDYVRYVCWYSE
                     TMVREAYAHHLKDMKEKLPAHLHPYLLTTLPPYIRETIPVSANEALVYERVQHIDPEG
                     YYILWVQNGVTRYRMEIRHKNPGFFDRSSGAVVNERVYR"
     gene            complement(5451..6620)
                     /locus_tag="PK856_02325"
     CDS             complement(5451..6620)
                     /locus_tag="PK856_02325"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRL01241.1"
                     /translation="MNGLNVLVCLVAVLGLANACVAEEPQAATNASSATSKTAIETQA
                     PFSTKALQTFHRPWAMAVLPDHKLLVSEKSGKLWWADLKTGLKHEIAGVPAVVDAGQG
                     GLGDLALHPDFARNQWLYFSYVEAGESHLQGAVVARAYLDLSADGGRLSDVKVIWRQV
                     PKVTGDGHYGHRLLFGADGKLWISSSERQKFTPAQDMNSNLGKIVRLNDDGSVPTDNP
                     FSDQGGVAAQIWSLGHRNVLGMAFDQQGRLWANEMGPKGGDELNLIEKGGNYGYPIVS
                     NGDHYSGRVIPDHNTRPEFIAPKISWTPVISPSSMVFYTGQQFPMWQGQLLIGGLSSK
                     SLLRIAIHGGEAEEVARYDMGERIRAVAQGQEGEVWLLEDGNTGRLLQLQPKAPR"
     gene            complement(6779..7225)
                     /locus_tag="PK856_02330"
     CDS             complement(6779..7225)
                     /locus_tag="PK856_02330"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HRL01242.1"
                     /translation="MLEILLVGIAVVACNVTIQALSTLYFMKIFIPQYKRWHEKNESL
                     CAFVMLVVLMMFFTLLHGGQCTLWAAVYYFNPEIGSLSSFSESVYFSLITFTTIGYGD
                     VVIDSDWRILAGLEAINGIILVGWSTAMVFSFLQIIYKNGKMPTFD"
     gene            complement(7374..8246)
                     /gene="accD"
                     /locus_tag="PK856_02335"
     CDS             complement(7374..8246)
                     /gene="accD"
                     /locus_tag="PK856_02335"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HRL01243.1"
                     /translation="MSWLDKILPPKIKSKDTSSRSSVPEGLWVKCPSCAAVLYATDLQ
                     QNMQVCPKCGHHHAISARERLNIMLDEEGRQEIGATVKPVDILKFKDSKKYPDKLTAA
                     RKATGEDDAMVVMSGKMSGLAVVIAVFEFKFIGGSMGSVVGERFVRGVREAVRLNCPF
                     ICVAASGGARMQEGLNSLMQMTKTAAALHLLSDKKLPFISILTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRRELNQSLLS
                     LLTLLNRSDAIKAA"
BASE COUNT         2042 a         2388 c         1887 g         2008 t
ORIGIN      
        1 ggcaaggcca aaggcagact gggttgatag gattgcacgc accaacgaca acacagccac
       61 gcaagcaaac gcggtaacaa accatacacc aacaaagacg accacaacaa attcgcccac
      121 tggcgcgaga atgccacttc gcccacgctt tgactggccg ccaccgtgtg cgcatcaggc
      181 acattcaaac ccaacacgct gggcaaccaa gccaagaccg ccaccaccgt gttcaaactg
      241 ccttggtcga gcagcgtcga ttgccagctg aacgtgtatt ggcgcaccaa caacatcatc
      301 accacaccca ataacaagcc gcccaaactc gccagccaca tgctgtggct gatgctgccc
      361 acccaccatt tcatttgcgg ctgcgccaat tgttcccgca acaaggccaa caaggcttgg
      421 tgctcggctt tgcgctgcgc ccccaaaccc atgctcgctt gcagccatgc aggcagatgc
      481 acttgtggct taaacaaata caccagccac cacacaaaca tcagcgcatt cacgcccagc
      541 acactgacga gcacataaaa gaaattcagc gccggcgttt gcatcaagcc gacggcggta
      601 aaaatgccca aaaccagcca caacacccac aacacctgtc gcaacaacac cgcacggctc
      661 aaaaactgtt gcaacaagcc atgcacgcgc tggtcgctgt ctatgcgctg cgcccgcagc
      721 cacagcttgc tttcaaaatc ggcatcgctg tgtttgagct cggtattgat gcgcgcaggg
      781 tcgctgggga acacatggtg ttgttcctcc aagcgccgca ccaactctat gtatgccatc
      841 cgtaaattaa tcacagcaac ccatcttgat tcagttgtgg cattgtatcg ggggaaaaca
      901 gatttagaca aatgtttaca ggccgcatca agcggcctgt aaggtttaga aacggtaaga
      961 agtgttcaca aataccgacg tttgcgcatt gggtgcatca cccacctcgg ccaatacccc
     1021 agcgccaacc tgccaacgct cggtaatatt gccgttcaca cccagctcca acacaccgct
     1081 gtttttagca tcacccgtat caacaggcaa ttcaaaatgc atgttgtcca cactgttcaa
     1141 acgcgcaccc accatatcgt ccacgtcctt gaattcgtgt tgccattgtg ccgaaccata
     1201 cacttgccaa gcattgcttg ccgcccattg cacttgatac cccaagccag attgaatgga
     1261 aatgtgctct tgctcatcaa aactcatggc cgtggattgc ttgccttgct cttgtaagga
     1321 tttgatcgtg gctttttgtg cgctcagaga cagcacaggg ccatgcgtaa atgccccttg
     1381 ctgccaattc atgccagcgc tgactttgcc accatactgg tcaccatcgg cactgccgtg
     1441 atggatgcgc tccgccgcac ccaattgcac acgacggtgg ctgtccacat ccaaattggc
     1501 gtaatagact tgaccattga gccacagatt ttggtgttgc caacggccat acaggccgcc
     1561 tgcccaacgc tgttcttcat agccgctgcc tttggccaat ttgccatcca aattatcaaa
     1621 agccaaatag gcacccgccg tccaattgcc catgcgtttg tccgcgccca acatccacgc
     1681 gctgcccgtt ccatccaatt ctgttcccaa ggcatcgcca ctgcccaaga ctgccgtgcc
     1741 ttgcccccac agtgcaaacc gctcatcttg aatgtgcgcg gtattgtgca aacggcggtc
     1801 tatgtgttgc atttgtgcaa tgcccgcttg attggccgca ggcgccaaca ccaccacttg
     1861 ttgtggtgcc gtcaacactg cggtggcgta atcggccaaa actttgtggc cagcagccgt
     1921 tggatgtacc ccatcggcaa acaaataggt ttggtcagca tgtgcttcta ccaaatcacc
     1981 agggccgcac tgtaaagacg aaacagcacc acaagcaggc gtggtgacat ttttcaaacc
     2041 aaattgtgat ggattggccg ccgcctcatg taacaaactc gccacatcca atggaatcac
     2101 attggctttc atgtcggcca attgtccata caaagcctga ttgtatgctg tgctcaattg
     2161 cgtgcctgcc gctacattgc cagaaaacgc aggagtcatg cccaaatcgg gaatgttggg
     2221 caccaaaata tagcgtgccc ctgcttgtga cagcgcactg accacagtaa cgttctcaat
     2281 gattgccgag gtgatggtgg tttgcgcagg tgcaccagcc gcaacagcaa acaaatcatt
     2341 ggcaccgccc cacaccgtgt ataaggcatc accgtccaag cgattgcctt caaccgctcg
     2401 caactgttgc aattgggtgc gcaccgatgg aatcggcact gtcacaccac caacagtggt
     2461 ggaactgttc acacccaccc gcgcaccacc tacggcataa ttggtgcctt ttttgccact
     2521 ggggtcggca gctgtgccca attcttcagc caattgttgt gaccaaacct tacctggatt
     2581 ggtggtaaag cgttgaccca tgaattgccc actgtcggtc aaactgtcac ccaaaaaata
     2641 ggtttgggaa aatggcccag cctctgccac agtgcccgca cccaatacca tcagcaacac
     2701 cgccaaatgt gtttttctca tgtttgctcc ttgtgttgtt ttttgttttc ttgttttctc
     2761 ctacggcttt gagcatagca gaaagccagc atccaccatg cggcctgtaa gcagagtgag
     2821 aaacaaacaa catatttaca acaccgctgc atcggtttat gttgtcgcca tgaacacacc
     2881 ccctctgaat atcgccattg ttggcagtgg catggcaggc ttggccgcgg cctatgtctt
     2941 gaaacaatcg ggtcaccatg tcaccttata cgaaagccaa gccaaaagcg gcttggatgc
     3001 acacagtaca acgtttgcag gcggcatggt ggatgcgccc ctgcgggtaa tgaatccaca
     3061 attgtggtcg cacacccttg ccttggcgga acaccttggc attcaaatgt atcgggtgcg
     3121 aacagacatg gcttgcagtt ggatggaggg tgaacgcatt ggggctacgt ggttcagcag
     3181 ccgcagacag gctcatgtgg ggttccccgt tttgggcagt tggcgttttg ccaaacagct
     3241 tcccgcattg gctttgggcg tgtatcagct caaacaggct ttgctgcaca cggaagggct
     3301 agccagcaca ctgagcttgg cagaatggat gcagcaatac cccatacacc cgctgctttg
     3361 gcatggctgt gtcgtgcctg tgctgcttac ggtgtgcacg tgtgatgttc agaccttatt
     3421 gcgttggcct gcacggccct tattggtgtt tgtggatttg ctgttgcgtg atgccggcct
     3481 gtaccgttta caaggtggca cacaggcgtt ggcaaatgcc ttaaagcaag gcgtgtcttt
     3541 gctgaaccac accagcgtca gtgcgataca ccaaaaccac gcgcagattt ttgtgtgtga
     3601 tgctgccgag cacacacaag ccttcgacca cgttatcgtc gctacgccca cgcaagcatg
     3661 tggttttttg gatgctgaac aattcggcct tgaaaaacag ctgctgcaac aactgcctta
     3721 cagcagcggt gaactcgtca gccacactga cagcagtgtg atgcctcgca agcggcgcga
     3781 ttggtcgcct ttgcattaca acatgcgccg tgatttctcc gcccacagct tcagcgtgtg
     3841 gctcaatccg atagaaacca gcttgtcaga gacggcgcca gctgtattcc aaagttggaa
     3901 cagcttaagt cccattgctg acaaaaccat tattcagcaa acctgcctga cccgtgccgt
     3961 ctgcactcct acctctttac aagtggtcaa gcaattacaa ggcttacagg ccgatgttca
     4021 gttgaatcaa cggcgtttgt ggttgtgtgg ctcatggagt tgtgagggtc tgccgatatt
     4081 ggagtcggca gtgacttctg ccttagccgt ttgcaaacaa ttgggtgccg tcttgcctgt
     4141 tgatttgccc tccacaagcc ctctcgcatg agttccaaac ggcgtgagcg catcaaccac
     4201 tcgattttgg gcgatgcccg caagcaagct tggagcaatt tgggtttgtg gcgaacaggt
     4261 caaagcgatt accgcactgc cgcacaagca ttggccttgc aattggcaga gcaaatgtcc
     4321 ttacaggccg cttcttcgct gttggatgta gcgtgtggct atggtgcttc gttgaatgtg
     4381 tggcacgaaa tgggtatccg cgaaatcaca ggattggagc cgcaaacagc gtgtgtgcaa
     4441 gcttggcaaa ccgaccatcg ttttcaatgt tggcatgaca gcatgcaaaa cattagcctg
     4501 ctctcgcaag accagccgtt tgaggccatc atcgccatag atgcggccta ccaatgggat
     4561 gtgcagctgt ggctgcaaca agcacatgca gcgctttgcc cgcatggtcg tttgggcttt
     4621 cattttttac tgcttgccga tgattttgcc caacggccgc tgtggcaaaa actctgtacc
     4681 cgcgccttat tgcgcatggt tggtgtcaaa tcttacctta cacaaaatca attacaggcc
     4741 gctttaaatg cccactttca tcaaacgtgg tcagaggtga gcatcaccga tttgagcgat
     4801 gaagtattgg gtggttttgg gcactatgcc ttgcgtttgc ctacataccc tcttgatttt
     4861 gaccacttga aaatacaggc caccggccat ttatgccgtt atttacaccg ccaaaaaagc
     4921 gtgcgctacg tcagcatctg cctgcaaaaa actgcgcccc gttaaacacc catcacatca
     4981 atggccctat gcctgttggt taacggtaga ctctttcatt caccaccgcg ccactgctgc
     5041 gatcaaaaaa accaggattt ttgtgacgta tttccatgcg atagcgggta accccatttt
     5101 gtacccataa aatgtaatag ccttctggat caatgtgctg cacgcgctca tacaccaaag
     5161 cctcgtttgc cgacacgggt atggtttcac gaatataagg gggtaacgtc gtcaataaat
     5221 aagggtgcag gtgtgctggc aatttttcct tcatgtcttt gaggtggtgg gcataagcct
     5281 ctcgaaccat tgtttctgaa taccagcaca catagcgcac atagtcttct ttgtgttttg
     5341 gctcatcaca tttcttgctc atcaaagccc atgtattggc actcatgcac agcaaaccca
     5401 gcaaggccac gtgtttcaac cgcatttcat tctcccatca ttcataggca ttatcttggt
     5461 gccttcggct gcaattgtag taagcgccca gtgtttccat cttccaataa ccacacttcc
     5521 ccctcttgcc cttgtgccac tgctcgtatg cgctcaccca tgtcatagcg agccacttct
     5581 tctgcctctc ccccgtggat ggcaattctt agcagcgact tggaagacaa gccaccgatt
     5641 aacagctgcc cttgccacat gggaaactgc tggccggtat aaaacaccat gctggatggg
     5701 gaaatcacgg gtgtccaaga gattttgggg gcaatgaatt cagggcgggt attgtggtcg
     5761 ggtatcactc gtccactgta atgatcgcca ttggaaacaa tggggtaacc ataattgccg
     5821 cctttttcaa tcagattcaa ctcatccccg cctttgggcc ccatctcatt cgcccacaat
     5881 cggccttgtt gatcaaaagc catgcccaat acattgcggt ggcctaaaga ccaaatctgt
     5941 gcggccacac caccttgatc ggaaaatgga ttgtctgtgg gcacactgcc atcgtcattc
     6001 aatcgcacaa ttttgcccaa attactgttc atgtcttggg ctggagtgaa tttttgccgc
     6061 tcactcgaag aaatccacag ttttccatcc gcaccaaaca acaatcgatg accataatgc
     6121 ccatcacctg tcacttttgg cacttgtcgc caaatcacct ttacatcgct caatctgccg
     6181 ccgtctgcac tcaagtccaa ataggctctt gccaccaccg caccttgcaa gtgcgattca
     6241 cccgcctcaa cgtaactgaa atacagccac tgattgcgtg cgaaatcagg gtgtaacgcc
     6301 aagtctccta agccgccttg ccctgcatcc accaccgcag gcacacccgc tatttcgtgt
     6361 ttcaatcctg ttttgaggtc tgcccaccac agcttgccag atttttcgct gaccaacagt
     6421 ttgtggtctg gcaacaccgc cattgcccaa ggcctatgaa atgtctgtaa ggccttagtt
     6481 gaaaatgggg cttgggtctc tatggctgtt ttcgatgttg cgctgctcgc gttggttgca
     6541 gcttgcggct cctcagccac acaagcattg gccaaaccca gcacagccac caaacacacc
     6601 aatacattca aaccattcat tgtgttttcc cctattttca ggctttgctc ccactgtcgt
     6661 gtcactcaat gcggcctata aacagcatac aagctgcatc aatcggttca cactgcgctc
     6721 acacccatac ccacagcttg caccacaaaa tcaccatgca cgcagaacca cgctggcatc
     6781 agtcaaacgt gggcattttg ccatttttat aaatgatttg taagaatgag aacaccatgg
     6841 cagtagacca cccgaccaag atgatgccat tgatggcctc caagcccgcc aatatacgcc
     6901 agtcagaatc tatcaccaca tcaccgtaac caatggtggt gaaggttatc agtgaaaaat
     6961 acaccgactc tgaaaaactc gacaagctgc ctatttctgg attgaagtaa tacaccgcag
     7021 cccacaaagt gcattgccca ccgtgcaata aggtgaagaa catcatcaac accaccaaca
     7081 tcacaaacgc acacaaagac tcattctttt catgccaacg cttgtattgc gggatgaaga
     7141 ttttcatgaa atacagtgtg gacaaggcct gtatggtcac attacaagcg acaaccgcaa
     7201 tacccaccaa gagaatttct aacatattcc aaactcgtgt tctcgtgctg ttttgaaaaa
     7261 atggcttgcc atcatcaccg ccctattgca ttaaaccatt tgctgacaca gatacaaaag
     7321 cggcctgtaa ggtttacagg ccgcttttca aatattgacg ctacatccaa tcatcaagct
     7381 gctttgatgg catcgctgcg attgagcaag gtcaacaaac tcaacaggct ttgattcagc
     7441 tcacgacggt cgacgatttg gtcgatggcg cctttttcca acaaaaattc tgcgcgttgg
     7501 aagccttcgg gcaaggtttc acgcacggtt tgttcaatca cgcgtgggcc tgcaaaacca
     7561 atcaaggcat tgggttcagc caagaccaca tcgcccaaga acgcgaaact ggcagacaca
     7621 ccacccatgg tagggtcagt caaaatggaa atgaaaggca attttttatc gctgagcaaa
     7681 tgcaaagccg ctgcggtttt ggtcatttgc atcaacgaat tcaagccttc ttgcatgcgc
     7741 gcaccacccg aggccgccac acaaataaac gggcaattca aacgcacggc ctcacgcaca
     7801 ccgcgcacaa aacgctcacc caccacagag cccatagagc caccaatgaa cttgaattca
     7861 aacacggcaa tcaccacagc caagccactc atcttgccac tcatcaccac catggcatcg
     7921 tcttcgcctg ttgctttgcg cgcggcagtc aatttgtctg ggtatttttt gctgtctttg
     7981 aacttcaaga tatcaacagg tttgaccgtg gcaccaattt cttgacggcc ttcttcgtcc
     8041 aacatgatgt tcaagcgctc acgtgcgctg atggcatgat ggtggccgca ttttgggcac
     8101 acttgcatgt tttgttgtaa atcggtggca tacaatactg cggcacacga tgggcatttc
     8161 acccacaaac cttcaggcac agacgaacgg ctgctggtgt ctttgctttt gattttgggt
     8221 ggtaaaattt tgtctaacca actcacgtca gtccccttat acggctgttt tcacagcttg
     8281 gttcaattct ttaacaatgg ctgcgactgc agtcgtttct tgttg
//
LOCUS       DAOMVK010000037         8299 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_2217_length_8299_cov_3.131126, whole genome shotgun sequence.
ACCESSION   DAOMVK010000037 DAOMVK010000000
VERSION     DAOMVK010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 8299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8299
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2217_length_8299_cov_3.131126"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="PK856_02340"
     CDS             <1..1131
                     /locus_tag="PK856_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRL01244.1"
                     /translation="RTFNRATQAWRQPGSTFKPFIYSAALAKGVTAATPVNDAPISLP
                     GMGSGGRPWEPKNSDGRYAGYISMHSALVASKNMVTIRLLMMLGVKNAKDYIQRFGFA
                     AKDHPDSLSMALGAGSATPMQMAEGYAVFANGGYKVTAYVIDKIYDGSGRLRAQTQPL
                     VAGQNAQQAIDPRNAFIMYTILKDVTRRGTAARANALGRSDIAGKTGTTNDMKDAWFV
                     GFNPKLVTAVYVGFDKPRTLGRAGYGGVIALPVWMEYMKFALKGTPMVEVKPPKGLVQ
                     HGGEYFMEEFQSLNPNLNIDNHASAPIKAEERDTETVGSDSNKGVTVDPINGLGEPGG
                     GTTVEPINGAGPQEVRPSNSTGQGGGNKPPPQQKTDAIDDLF"
     gene            complement(1538..1885)
                     /gene="erpA"
                     /locus_tag="PK856_02345"
     CDS             complement(1538..1885)
                     /gene="erpA"
                     /locus_tag="PK856_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HRL01245.1"
                     /translation="MSDVETSAAIVFTDSCCTKVKDLIAEENNPELKLRVFVNGGGCS
                     GFQYGFTFDEIVNSDDFEIKKDELTFLVDPMSYQYLVGAEIDYTESLHGSQFVIRNPN
                     ATTTCGCGSSFSV"
     gene            complement(1907..2959)
                     /gene="argC"
                     /locus_tag="PK856_02350"
     CDS             complement(1907..2959)
                     /gene="argC"
                     /locus_tag="PK856_02350"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRL01246.1"
                     /translation="MSKSKTQVGIVGATGYTGVELLRLLAHHPEVEVHTVTSRQEAGK
                     AVADYFPSLRGAYDLVFQAPNDADLSQCDVVFFATPNGVTMKEAPALLNQGIKVVDLS
                     ADYRIQDVATWQAYYKMDHASPEWIAKAVYGLSEWKQAEIATAQLIANPGCYPTCVGL
                     PLLALLQNNSLKADANIIADCKSGVSGAGRKAEIGSLFSEAGDNFKAYGVAGHRHLPE
                     IRQTISAFSPEIAQRFTFVPHLTPLIRGMHATIYVELNEGVDALQVLQAAYQDSVFVD
                     VMPQGSLPETRSVRGANTCRITAYRAPETKANQWIILSVIDNLVKGAAGQAVQNMNIM
                     LGLPEQTALNGMPLLP"
     gene            complement(3099..3491)
                     /gene="rpsI"
                     /locus_tag="PK856_02355"
     CDS             complement(3099..3491)
                     /gene="rpsI"
                     /locus_tag="PK856_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRL01247.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLQKGTGQIIVNGRPVDEFFSRETG
                     RMVVRQPLTLTENLESFDIKVNVCGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(3501..3929)
                     /gene="rplM"
                     /locus_tag="PK856_02360"
     CDS             complement(3501..3929)
                     /gene="rplM"
                     /locus_tag="PK856_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRL01248.1"
                     /translation="MKTFSAKPHEVKREWYVVDAQDKVLGRLAAEIAHRLRGKHKPEY
                     TPHIDTGDYIIVINAEKLRVTGNKALDKKYYRHSGYPGGIYERNFTEMQQQFPERVLE
                     KAVKGMLPKGPLGYAMIKKLKVYAGADHGHEAQQPKVLDI"
     gene            complement(4180..4358)
                     /gene="ssrS"
                     /locus_tag="PK856_02365"
     ncRNA           complement(4180..4358)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PK856_02365"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(4374..4664)
                     /locus_tag="PK856_02370"
     CDS             complement(4374..4664)
                     /locus_tag="PK856_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HRL01249.1"
                     /translation="MSISQVDVNILGRQFTIGTPDEEAETLRQAVYLLNQKIGVIQDT
                     GRIVETDKIVIMAALNLAHDFLKMTTKDGLEFAQFERKISDMITACEKTLAE"
     gene            complement(4661..4996)
                     /locus_tag="PK856_02375"
     CDS             complement(4661..4996)
                     /locus_tag="PK856_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01250.1"
                     /translation="MENQLEHLQICLNALIDKFNAQSGDNASLTSRIQELEQEKRQLI
                     EQYDAQLASKEQVHTEQVNTLQTTSDKQISDLKVENTVLRATLIDTADAIKTLLSRLP
                     KVVQEEIEQ"
     gene            complement(5029..5880)
                     /locus_tag="PK856_02380"
     CDS             complement(5029..5880)
                     /locus_tag="PK856_02380"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HRL01251.1"
                     /translation="MQKILIILWMSCLTVLSQAAISVQDGTGQTVTILHEIRKVVALT
                     PHAAELAYSAGAGSKLVGVTQESDYPEPVKALPKVGTYVQINLESIIKLKPDLVIAWQ
                     DGGQAPLIQALHQAGIPVFMSHPLSPQDIATEIHALGVLNGTPNIAKQNTAPFRLYLK
                     TLEQTPKTPTVNSMFLVGYNPIYAISDNGFLGKMMQACGANNVFGHIKQPAFTLNPEA
                     LLQQTAQIAFISNEGDLKQWQAFYSKSRQPPKIHSINADLRPSLRIKSSLEQMCLIID
                     TERRSRH"
     gene            complement(6329..8197)
                     /locus_tag="PK856_02385"
     CDS             complement(6329..8197)
                     /locus_tag="PK856_02385"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRL01252.1"
                     /translation="MQIKPIAAALALLACQSAAYADTTSAETELNDIVVTATRVSQNP
                     LKSLNDVSVITRSDIDNSNAQSLPELLQQTIGMETVSNGGSGTVSSLYTRGTNTNQTV
                     ILIDGVRLVSATTGAASYNHIPLNQIERIEVVRGAASSVYGADAIGGVVQIFTRKPKD
                     GAIHGSIGAKVGTDGQRGVDANFGGRTQGFSYQISAGTERTDGGFSSTNSDYDSAFSV
                     HNPDEDGYKNQNVSGRLAYEWAEGHEISASALRTRGDAEFDEGASDPTTWMPIAVDNE
                     HYITDLSQFTIASRDKITDSIQSNLQLSISREKGKSQTDEKTNYYVNSKDKRAQWIIS
                     GDHEHVQWQVGTEWLKQQVDSNTVYVRDSRINRAFFGAVNGNFNHFLIESSLRQDRDS
                     QYGSHTTGRLALGYEITDQLVGRVSYGTAFRVPTFNELYYPADPFFGGGGNPDLNPEK
                     SRSWEAGLKYHTQDLALDWTVFHTKLTDMINGWPAQNVNDATMVGTSLAASKQWQQVK
                     LYGNVTYQDPRDKTNERLLVRRARVFGNIGVEYQPHEQWRLNANMHVHGRSYADAANT
                     DSLGGYAILGAGVEYKPSEQWSVKLSGNNLLDKQYTTVRGYNTQGRSVLLSGRYSF"
BASE COUNT         2061 a         1790 c         2025 g         2423 t
ORIGIN      
        1 cgtactttca accgtgctac ccaagcgtgg cgtcagccag gttcaacatt taagccattt
       61 atttactctg ctgccttggc caagggtgtc actgcagcta ctccagtcaa tgatgcacca
      121 atttctttgc cagggatggg ttctggtggt cgaccatggg agccgaaaaa ctcggatggt
      181 cgttatgctg gctatatttc catgcactca gctttggtgg cttctaaaaa catggtcacc
      241 atccgcttgt tgatgatgtt gggcgtgaag aatgccaaag attatattca gcgttttggt
      301 tttgccgcca aagaccatcc cgatagtttg tccatggcct tgggtgcggg ttccgccaca
      361 ccgatgcaaa tggcggaagg ctatgccgta tttgccaatg gcggctataa ggtaacggct
      421 tatgtcattg ataagatata cgatggttca ggccgcttgc gtgcgcaaac ccagccattg
      481 gtagcagggc aaaatgcgca gcaagccatt gatcctcgca atgcctttat catgtatacc
      541 attcttaaag atgtgacacg tcgtggtacc gctgcacgcg ccaatgcatt gggtcgttct
      601 gatattgcgg gtaaaacggg taccactaat gacatgaaag atgcctggtt cgtgggcttt
      661 aaccctaaat tggtcactgc tgtctatgtg ggttttgaca agccgcgtac gcttggtaga
      721 gcaggttatg gtggtgtgat agccttgcct gtgtggatgg aatacatgaa gttcgcgttg
      781 aaaggcacgc ctatggtgga agtgaagcca cctaagggct tggtgcagca tggtggcgaa
      841 tactttatgg aagaattcca aagtcttaat ccaaacctga acattgacaa tcatgccagt
      901 gcgcctatca aggcagagga gcgcgacact gagactgtgg gtagcgacag taataaaggc
      961 gtgactgttg atcctatcaa tggtttgggt gaacctggag gtggtaccac tgttgagccc
     1021 attaatggtg ctggcccgca agaagtgagg cctagcaact ctactggcca aggcggtggc
     1081 aacaaacctc caccacagca aaaaaccgat gcaatagatg atttgtttta agtaaatgtg
     1141 acatctcaag cggcctgtaa gtgtttacag gccgcttttt tgtgtgcgaa gaacttgaat
     1201 gataggtgag aaaggtttga gtttaaattg atgctgttta tgcattattt ctcactttac
     1261 caaaataatt ccatatatct accagtatgt cttttcatct ggtttgatgg ggtgttttca
     1321 cttaagctaa atggtttgaa gttgtgaaag tcaatgtaag tggcgctagt aggatgcacc
     1381 gcattttacg atgtaaaggt aaggcttgag aaagtatggc agaagtgggt aggatttaag
     1441 ataaacttat gggattgtgc taaatgaata tcatcatgat gtgcgtacta aagaaagcgg
     1501 cctgtaaaca ttacaggccg cttattagat tgttgtctta aactgagaaa gaagagccgc
     1561 agccacatgt ggtggtagcg ttggggttgc gaatcacaaa ttgtgagcca tgtaggcttt
     1621 cagtgtaatc aatttccgcg cctaccaggt attggtagct cattgggtct actaagaaag
     1681 tcaattcgtc ttttttgatt tcaaaatcgt cggaattaac gatttcatca aaggtaaagc
     1741 catattgaaa gcctgagcag ccaccgccgt ttacaaacac gcgcagtttc aattctggat
     1801 tgttttcttc tgcaatcaag tcttttactt tggtgcagca gctgtctgta aaaacaattg
     1861 ctgcgctggt ttcaacatca ctcatagtgt ttcctttcat gtggtgttaa ggcagtaggg
     1921 gcataccgtt caatgcggtt tgctcgggta ggcccagcat aatattcata ttttgtacgg
     1981 cttggcctgc tgcgcctttg accaaattat caatgacaga caatataatc cattggttgg
     2041 ccttggtttc aggggcgcgg taggcggtaa tgcggcaagt gttggcacca cgcactgagc
     2101 gtgtttcagg caagctgcct tgtggcatga catccacaaa cacgctgtct tggtaggcgg
     2161 cctgtaacac ttgtagtgca tccacacctt cattcaactc cacatagatg gtggcatgca
     2221 tgccgcgtat caagggggtg aggtggggga caaaggtaaa tctctgtgct atttcaggtg
     2281 aaaaagccga aatggtttgg cgtatttcag gcaaatgacg gtggcctgca acgccatatg
     2341 ctttaaaatt gtcacccgct tcgctgaata ggctgccaat ttcagctttg cgtcccgctc
     2401 cagatacgcc tgatttgcaa tcagcaatga tgtttgcatc agcttttagg ctgttgtttt
     2461 gcagtaaggc caataaaggc aagcctacgc aggttggata gcagcctggg ttggcaatca
     2521 attgggcagt ggcaatttca gcttgtttcc attctgatag gccatatacc gctttggcaa
     2581 tccattcagg acttgcatga tccattttgt aatacgcttg ccaagtagcc acatcttgaa
     2641 tgcgataatc agccgataaa tccacaacct taatgccttg gttcagtaag gcgggggctt
     2701 ctttcatggt cacaccatta ggggtggcga agaaaaccac atcacactgg ctcaaatcag
     2761 catcattggg tgcttgaaac accaaatcat atgcgccgcg caagctaggg aaataatcgg
     2821 ctactgcttt gccagcttct tggcggcttg tgactgtatg gacttctact tctgggtgat
     2881 gtgccaatag gcgtagtaac tccactccag tgtagcctgt tgcgccaaca atgcctactt
     2941 gggttttgct tttgctcatt atctgtcctg tcatcgtcat tcatgtctat tttacgctgt
     3001 attgcaacat agggggaatg cttgtgtgta tcggactgat agttaagcac aaataaaaaa
     3061 ccgcccgaag gcggcttttc catgcaaaac gtaaaaaatt aacgttttga gaattgtttt
     3121 gcgcgacgtg ctttgcgtaa accaggtttt ttacgctcaa cttcacgggc atcgcgggta
     3181 acgaagcctg cttgagacaa cgctggtttc aatgctgcat cgaagtcgat caaagcgcga
     3241 gtgataccgt gacgaatggc accagattgg ccagtttcac cgccgccaca tacgtttact
     3301 ttgatgtcaa agctttccaa gttttcagtc aaagtcaatg gttggcgaac caccatgcga
     3361 ccagtttcgc ggctgaaaaa ttcgtctact ggacgaccat taacaatgat ctgaccagta
     3421 cctttttgca aaaatacacg tgctactgaa cttttgcgac ggcctgtgcc gtagtagtat
     3481 ttaccgttca tcgtgtatcc ttaaatatcc aaaactttag gttgttgtgc ttcgtgaccg
     3541 tgatcggcac cagcatacac ttttaacttt ttgatcattg catagcccaa aggaccttta
     3601 ggcaacatgc ctttcactgc tttttccaac acgcgttctg ggaactgttg ttgcatttca
     3661 gtgaaattgc gctcgtagat accaccaggg tagccagagt gacggtagta ttttttgtct
     3721 aatgctttgt taccagttac acgcaatttt tcagcattga taacgatgat gtaatcacca
     3781 gtatcgatgt gaggagtgta ttctggtttg tgtttaccgc gtaagcggtg agcgatttca
     3841 gcagccaaac gacccaaaac tttgtcttga gcatcaacta cgtaccattc gcgcttcacc
     3901 tcgtgcggtt tagctgaaaa ggttttcata ggaatagtcc aaaaatagtg aatactgtaa
     3961 accgtcgatt ttatgtgaga gtcggcttac tgtcaatata aacaaaggac ttgctttaaa
     4021 gatttgcaca gcttgtggtg agaattaagc aatttgagca ctggagcaca aagatttgag
     4081 tgaaatccaa gtgcatattg gtgggattta cttaggaagt gggaacttaa actagagatg
     4141 aggctaaaat caagctgttt agtggagaag taggatgggg aatcccccgc tggtgccggt
     4201 aggcataatt cgcttgaacc ttggggacaa ggctagtcac atgggtatgt ttcgggtgcg
     4261 ttcgcgagga acgctcgcgc ccacatacca ctttgcaact ataaagcgaa cttattggtt
     4321 caaaggaatg taagcccttt cgaacactgc aggggaaaat ttaactgttt agattattca
     4381 gccagtgttt tttcacaagc tgtaatcatg tcgctaattt tacgctcaaa ctgcgcaaat
     4441 tccaagccat ctttggttgt catcttcaaa aagtcatgcg ccaagttcag ggcagccatg
     4501 atgacgattt tgtcggtttc cacgatgcgg cctgtgtctt gaatcacacc tatcttttga
     4561 ttcaataagt aaacggcttg acgcagggtt tctgcttctt cgtctggcgt accgatggtg
     4621 aattggcggc ctaaaatatt cacatcaact tgtgaaatac tcattgttca atctcctctt
     4681 gtaccacttt aggcaggcgt gacaacaagg ttttaatcgc atcagcagtg tcgattaaag
     4741 tggcacgcaa gacagtattt tcaactttta aatcgctgat ttgtttgtct gaagtggttt
     4801 gcaaggtgtt tacttgctca gtgtgcactt gctctttgct tgccaattga gcatcgtatt
     4861 gctctatcaa ttgacgtttt tcttgctcca gctcttgaat gcggctggtc aaggaagcat
     4921 tgtcgccact ttgcgcatta aacttatcga ttaaagcgtt taagcagatt tgtaagtgtt
     4981 ctaattgatt ttccatggct cagagaatat cgtagtggga tgagtaaatc aatgccggct
     5041 tcggcgttcg gtgtcaatta taaggcacat ttgttctaat gacgacttaa tacgcaaact
     5101 tggacgtaaa tcagcgttaa tgctgtgtat tttaggaggc tgacgagatt tggaataaaa
     5161 agcttgccat tgttttaaat caccttcatt gctgataaat gctatttgtg cggtttgttg
     5221 taataaggct tctggattca aggtgaatgc aggttgtttg atgtgaccaa acacattgtt
     5281 ggcgccacac gcttgcatca tttttcctaa aaaaccgttg tcactgatag cataaatggg
     5341 gttgtaacct actaaaaaca tactattgac ggttggtgtc ttaggcgttt gttccaaggt
     5401 ttttaagtac aaacgaaatg gcgcggtatt ttgctttgca atattgggcg tgccattgag
     5461 tacgcccaaa gcatgaattt ctgtggcaat atcttgtggg gagagtgggt gcgacatgaa
     5521 aacaggaata cctgcttggt gcaatgcctg tatcaatggc gcttgcccac catcttgcca
     5581 agcgatgacc aaatctggtt tgagcttgat gatggattcc aaattgattt gcacataagt
     5641 acccactttg ggcagtgctt tgacaggctc agggtaatcg ctttcttgag tgacgcctac
     5701 cagtttgctg ccagcccctg cgctgtaggc caattcggca gcatgtggtg tcaatgctac
     5761 gaccttgcgt atttcatgca gaatggtgac ggtttgtccc gttccatctt gtacgctgat
     5821 ggcagcttgg ctgagcactg tgaggcagct catccataag attatgagta ttttttgcat
     5881 aatgtctcga tttacaggcc gcatatttcc tattggaaac ttgaaacaca atgcttgtac
     5941 caacactttt aagctttgtt tcattcagtg ttgcactagt gatgctaatt gcagagtgat
     6001 agcgagtagc gtttttgagt acaatgtatt tgtgctgcat gtgtcagaat gatggtatcc
     6061 gcatccagat tgctgtttga gctgaaagat ggctaccact tcgtatcgtt ccatctgcgc
     6121 ctttgtgcta ttggacactt atttaagtgt atcgctctgt tattttggta atgcaatctg
     6181 tttttcggaa tgcatgcaat gctgtgcaat aggaaaagat attttaaaga atatatgttt
     6241 tagcaccatc atttaattta caggccgctt atgacacaca tgcggcctgt aaatgtttct
     6301 aagtgttttt tctaaagctt aaacgcactt agaaactgta acgtcctgac aacaatacac
     6361 tgcggccttg agtgttgtat ccgcgcacgg tggtgtattg cttgtccaac agattgttgc
     6421 cagataattt tacagaccat tgttcagatg gtttgtactc tacacctgca cccaatatgg
     6481 cataaccacc caaactgtca gtattagcag catctgcata gctgcggcca tgcacatgca
     6541 tgttggcatt caagcgccat tgctcatgcg gctggtattc aacaccaata ttgccaaaaa
     6601 cgcgagcgcg tctaaccaat aatctctcat tggttttgtc gcgaggatct tggtaggtca
     6661 cgttgccgta caattttact tgttgccact gtttactggc ggcgaggctg gtgccaacca
     6721 tggttgcgtc attcacgttt tgagctggcc agccattgat catgtccgtg agcttggtgt
     6781 ggaacactgt ccagtctaag gccaaatctt gggtgtggta tttcaggcct gcttcccatg
     6841 agcggctttt ttcagggttt aagtcaggat tgccgccgcc accaaaaaat ggatcggctg
     6901 gataatacaa ctcattgaaa gtagggacac ggaaggctgt accataactc actcgaccaa
     6961 ccaattggtc ggtaatttca tatcccaagg ccaggcgacc agtggtatgg ctgccatatt
     7021 ggctgtcacg gtcttggcgc agagaagatt cgatgagaaa gtgattgaag ttgccattga
     7081 cagcaccaaa gaacgcacgg ttgatgcgag aatctcgaac gtagacagtg ttggagtcga
     7141 cttgctgctt cagccactcg gtacccactt gccactgtac atgctcgtgg tcaccactga
     7201 taatccactg tgcccgtttg tctttgctgt tgacatagta attggttttc tcgtcggttt
     7261 gacttttgcc tttttcacgg ctgatggaca attgcagatt agattggatg ctgtcggtga
     7321 ttttgtcacg tgaagcgatg gtgaactgag acaagtcggt aatatagtgc tcattgtcaa
     7381 cagcgatggg catccaagtg gtagggtctg aagcgccttc atcgaattct gcatcaccac
     7441 gagtgcgcag cgctgaagcg ctgatttcat gaccttctgc ccattcatat gctaagcggc
     7501 ctgaaacatt ttggttttta taaccatcct catcagggtt gtgtacgctg aaggcagagt
     7561 catagtcact attggttgat gagaaaccgc cgtctgtgcg ctctgtgcct gcacttatct
     7621 ggtaactgaa gccttgtgtg cggccgccaa agttagcatc gacaccacgc tgaccgtcag
     7681 tgccaacttt ggcaccaata ctgccatgaa tggcaccatc tttgggcttg cgagtgaaaa
     7741 tttgcaccac accaccaatg gcatccgcgc cataaacgct gctggctgca ccacgcacca
     7801 cttcaatgcg ttcaatttgg tttaaaggta tgtgattgta agaggccgct cctgtggtgg
     7861 ctgataccaa acgcacgcca tctatcaaaa tcacggtttg gttggtattg gtcccgcgag
     7921 tgtacaagct gcttaccgtg ccactgccac cattggaaac ggtttccatg cctatggttt
     7981 gttgcagtaa ttcaggcaga gattgggcat tgctgttgtc tatgtcactg cgggtaatca
     8041 cgctgacatc attcaggctt tttaatgggt tttggctgac gcgagtggca gtgaccacaa
     8101 tgtcgttgag ttcggtctcg gctgaggtgg tgtccgcgta tgccgctgat tggcacgcga
     8161 gcagcgctaa agcggctgcg atgggtttta tttgcatgaa acactccaag tattgtgtat
     8221 accgagtgcg aaagaatgtg tatttaaccg caatgaccaa acataacagt caaatatcgc
     8281 ctccccgcga tactcgtgt
//
LOCUS       DAOMVK010000038         8285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_2222_length_8285_cov_3.140583, whole genome shotgun sequence.
ACCESSION   DAOMVK010000038 DAOMVK010000000
VERSION     DAOMVK010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 8285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8285
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2222_length_8285_cov_3.140583"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="PK856_02390"
     CDS             complement(<1..650)
                     /locus_tag="PK856_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRL01253.1"
                     /translation="MKIVILGAGVIGVTTAWYLSQEGHQVQVIERHGHAACDTSFANA
                     GQISFGYSTPWAAPGIPQKALKWMFKEHSPLLMRPDASLYQLKWMMMMLQNCGEKVYE
                     INKERMLRIADYSRERFAALRAETGIEYEGRQQGTLQVFRTEHQLEVVENDMKVLAEC
                     GIPFEKLSPEACAAAEPALGRVQHKLTGGLRLPEDETGDCYTFTNRLAQMCQDAGV"
     gene            809..1291
                     /locus_tag="PK856_02395"
     CDS             809..1291
                     /locus_tag="PK856_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HRL01254.1"
                     /translation="MTLPAPTLDKIDMKIIQLLQLNARMSIADLADKVGLSATPIKER
                     MRRLQDQGVITGYHAKVNPHLIGQGLLVFVEIKLKDKSASLFAEFKRDVLRISQIMEC
                     HLVSGEYDYLIKVRMTDIAAYRQMLGDILLQLPAILESRSYIVMEEVKETHLLPMSNT
                     "
     gene            complement(2174..3625)
                     /gene="tldD"
                     /locus_tag="PK856_02400"
     CDS             complement(2174..3625)
                     /gene="tldD"
                     /locus_tag="PK856_02400"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HRL01255.1"
                     /translation="MSTPDVFQHASEHLLARNGISATVLDRILAEVGQHQVDFADVYC
                     QHSAFESWQLEEGIVKSGSFNIDEGFGIRAIVGDKTAFAYADSIVPESLKQAASTVRA
                     IARQGQSNAMRGAQVSTQVLAQYPMLNPMHSLDATQKVMLLEQVEALARAKDPRVVQV
                     MATLACEHDIVYIARLDGRHAADVRPLVRLSVTVIVEQNGRREQGSSGGGGRFDFAYF
                     DAAMVQYYVDKAVDQALVNLEARPAPAGAMTVVLGNGWPGVLLHEAVGHGLEGDFNRK
                     ETSAFSNKIGQQVAAKGVTVIDQGNIEKRRGSLSLDDEGNATGATVLIEDGILKNYLQ
                     DETNARLMGMAVTGNGRRESYASTVMPRMTNTYMANGSHDPDEILASVDKGIYAVNFG
                     GGQVDITSGKFVFSASEAWWVENGKLQYPVKGATIIGNGPDVMQHISMIGNDMALDSG
                     VGVCGKEGQSVPVGVGQPTLRIDEGLIIGGSQA"
     gene            complement(4062..4808)
                     /locus_tag="PK856_02405"
     CDS             complement(4062..4808)
                     /locus_tag="PK856_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01256.1"
                     /translation="MQLLKWLFAVLVVLNLIVFANVVTQKMNGNRPKQNPAVVAVPIG
                     GGTDASGNPIPAQNAGTTSGSRVVAPAITRRPAAVAGGGGGNTTLETAALPKNAPSCS
                     AQITLPEDAYHRLKNFIGGWNHAASRNVVENKQKGKASGVQYQVLVAGSSDEALQQLR
                     SAGISASPQGGSISLGVYPDAGKANSVRDRAIAAGVGGVIVSERGSAQSALTSAQYTV
                     SFRNITNQDAQKINEVLGRYGRLQRSACAQ"
     gene            complement(4796..6721)
                     /locus_tag="PK856_02410"
     CDS             complement(4796..6721)
                     /locus_tag="PK856_02410"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRL01257.1"
                     /translation="MNRWANHAWNLWGALADGKRQHVRDLATLLGCDAHALNGHWKQL
                     PAEVQKHLRQQDGWWQLKQAMAVVSPLQAASVSDSSGFQVDVLPETTSTNDVLWQRWQ
                     EGYNIHKHMVCALVQSQGRGRLQRPWQNVLGQTLMFSLAYQVEQGSAVLAALPLLVGL
                     LVQQVLAEQQVHAQLKWPNDIVVDSRKLCGILVESKQRGERWHVVIGIGVNVMQPQQE
                     ALREIATACNAQTADFSCTSFLAQLLPQLDQAMQTFFKQGFEVFQAAYTAQMRDLNQP
                     VNLFERGKLIGQGQMLGVNSMGGLRVQDAAGQEQVYLNGEISMRPEHASDVPQTVTPS
                     AMPVAEPEPILDMQDVSLFNTSAHLETVVTAKTTTNEHFVMPKPVSNQGKDRKQAGAA
                     RYILLDGGNSQLKWAWVDAEQQLHFGGRAPYANLQVFAEFMAAQPQDLPLIGCAVCGA
                     KKMALVEEAATRSVRWLPSMRHALGVTNHYYKVQQHGSDRWFNVLGSRLFSQNSCVVV
                     SCGTAITIDAITHDNHYLGGSIMPGFNLMKEAMAAKTANLNQPIGKAYPYATSTPNAL
                     ASGMHDAASGAVILMHQRLKERQQGQPVDLILTGGGASKIEQHLPKALILDSQVKIVD
                     NLVLFGLQNWVEHTCNY"
     gene            complement(6718..7215)
                     /gene="rfaE2"
                     /locus_tag="PK856_02415"
     CDS             complement(6718..7215)
                     /gene="rfaE2"
                     /locus_tag="PK856_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HRL01258.1"
                     /translation="MQHFPAPVFEHKICAPEELSTRIAQLAQPLVFTNGCFDILHRGH
                     VTYLAQARALGGSLVLALNTDASVKRQGKGDDRPINGLENRLAVVAALQSVDLVTWFD
                     EDTPANLIELIQPQVLVKGGDWAIEHIVGAKETQARGGKVVSIAFEHTTSTTQTLAKI
                     REAQA"
     gene            complement(7257..>8285)
                     /locus_tag="PK856_02420"
     CDS             complement(7257..>8285)
                     /locus_tag="PK856_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRL01259.1"
                     /translation="LDIVGDVHGEWAVLQQLLRHLGYDERGEHLQGRRLVFIGDIVDR
                     GPDSPAALAWVMRAREQHGVLSVLGNHELNILAGDPKDGSAWFFDSRMPEDTQYLPYQ
                     RLPIDERSGVLADLQTWPLVLQREDLRLVHAAWLPESINAIHGLDPERNIAQWYNYFD
                     EHVHELVHNKPWYPQYQQEYQQYDALLGKEDLHPPMLSGHTEFELSRWKQNPVRALVS
                     GSEIPVSEPFYAGARWRFTGRSAWWEDYHDDVPVVMGHYWRLWQNHTAAKTRHAGLMP
                     EDGSVWFGAKNNVFCIDFSIGARWRDRQQNIAPAQSKFHLAALRWPERTIVMDNGAQY
                     ASTGFEAA"
BASE COUNT         2174 a         2270 c         1758 g         2083 t
ORIGIN      
        1 tccacacccg cgtcttgaca catttgcgcc aaacggttgg taaacgtgta gcaatcgccg
       61 gtttcgtctt ctggcaaacg caaaccaccc gtcaatttgt gttgcacacg gcccaaagca
      121 ggctcagcag ccgcacaagc ttctggcgac aatttctcaa atggaatgcc acactcagcc
      181 aacaccttca tgtcgttttc aaccacttcc aattggtgct cggtacggaa cacttgcaaa
      241 gtaccttgtt ggcggccttc gtattcaatg cctgtttcag cacgcaaagc agcaaaacgc
      301 tcacgactgt aatccgcaat gcgcaacatg cgctctttgt tgatttcgta aactttctca
      361 ccacagtttt gcaacatcat catcatccat ttcaactggt ataatgatgc atctgggcgc
      421 atcaacaatg gagaatgttc tttaaacatc cacttcaatg ctttttgggg aataccaggc
      481 gcagcccaag gcgtggagta accaaatgag atttggcctg cattggcaaa actggtgtca
      541 catgctgcat gcccgtgacg ctcgatgact tgaacttggt ggccttcttg gctcaaatac
      601 catgccgtcg ttaccccaat cacaccagcg cctaaaatca caattttcat aaccattcct
      661 ctgtatagac tgtgccgcat tgttgcaaag caacaatttc gcagcttatt tatatagttt
      721 atcggataat gtgtggtgaa tttcactaaa aaaatggaat ttacgatata ttaattatta
      781 tttttaactt ttaaaatttg aattcaccat gaccctaccc gcacccacac tcgataaaat
      841 cgacatgaaa atcatccaac tgttacagct caatgcccgc atgagcatcg ccgaccttgc
      901 cgacaaagtt ggactctctg ccacccctat taaagagcgc atgcgtcgcc tacaagacca
      961 aggagtcatt actggttacc acgccaaagt gaatcctcat ctaatcggcc aaggcctatt
     1021 ggtatttgtg gaaatcaaac tcaaagacaa atcggccagc ctgtttgctg agttcaaacg
     1081 tgatgtgttg cgcatctcac aaatcatgga gtgccatttg gtgtctggtg aatacgatta
     1141 cttgattaaa gtccgcatga cagacattgc cgcataccgt caaatgctcg gcgatatttt
     1201 attgcaatta ccagccattt tagagagccg ctcgtatatt gtgatggagg aagtcaaaga
     1261 aacccattta ttgcccatga gcaatacctg agtatgatga aattggtcat taactgacaa
     1321 actggcaaaa aaaatgcgca catttgttta aatgtgcgcc aagtctacaa aggagaaata
     1381 gaggagaaac tgttaaacca gctatgcact gattcaacag ttgctattat ctattccaca
     1441 cgaatgaaag tcaacatagt gaaagtaaat ttacgatagc atttgataaa aatcatatct
     1501 actttaatga aaaaataaaa atataatcat aacttactga taatattgaa tataaattta
     1561 attcacaccc aataaaatgt tttgatattt gttgcttttc aatgtgaatg aacagacatt
     1621 cacaaggaat gttgtgttaa ttgttgcaaa gcaaaaaaat gcgcacattt aataaaatgt
     1681 gcgccaagtc tacaaaggag aaatagaaga gaaactatta agtaggtcat tgcaaccact
     1741 caatgaagcg tattatgaac acatatgaaa aaactgtcaa acattatttt catttttatt
     1801 tgttaaattt atgtaaatcg aaaatattaa caagcaatct cgagaataca ataaattcag
     1861 ttccaaatcg aaacaaaacg aacaatttta ataaatgttc tataatttca cttctgaaac
     1921 gaacattcca aacattctat agtgattctg cttgcttttg catgcctcag aatcaccatc
     1981 cacttcaagc ccagcaacga acaaactaac ccgctaagcg cttacaatca aaaaaccact
     2041 catacatttt ttctactcag tgcttcattc agaaaactga agctgtttca tcttatctgt
     2101 ttgtaaccac acaaagaaag cggcctgtaa tgtttacagg ccgcttaatg tatttactgt
     2161 tgatggatgc gtatcaggct tgagagccac caatgatcaa accttcgtca atgcgcaaag
     2221 taggttgtcc cacgcccaca ggcacgctct gcccctcttt accacacaca cccacgccac
     2281 tgtccaaggc catgtcgttg cctatcatgg aaatgtgctg catcacatct gggccattgc
     2341 caatgatggt ggcccctttc acagggtatt gcagcttacc gttttccacc caccacgctt
     2401 ctgaggctga aaacacaaat ttgccactgg tgatgtccac ttgcccgccg ccaaaattca
     2461 ccgcataaat gcctttgtcc accgatgcca atatttcatc aggatcatgg ctgccattgg
     2521 ccatgtaggt attggtcatg cgcggcatca ctgtagacgc atagctttca cggcggccat
     2581 taccagttac tgccatgccc atcaaacgtg cgttggtttc gtcttgcaag taattcttca
     2641 agatgccgtc ttcaatcaac acggtagcgc ctgtggcatt gccctcatca tcaagactca
     2701 aagagccgcg gcgtttttca atattgcctt gatcgatgac agtaacgcct ttggcagcca
     2761 cttgttgacc gattttattg gagaacgcac tggtttcttt gcgattgaaa tcgccttcca
     2821 aaccgtggcc caccgcctca tgcaacaaca cgccaggcca accattgccc aacaccaccg
     2881 tcattgcgcc tgcaggcgct ggacgcgctt ccaaattcac caaagcctgg tctaccgctt
     2941 tgtccacata gtattgcacc atggcggcat caaaataagc gaagtcaaag cgcccaccac
     3001 caccgcttga gccttgttca cggcgaccat tttgctccac aatcaccgtc accgataaac
     3061 gcaccaacgg gcgcacatcg gccgcatgac ggccatccaa gcgcgcaatg tacacaatgt
     3121 catgctcaca cgccaaagtc gccatcactt gcaccacacg cgggtctttc gcacgcgcca
     3181 aagcctcaac ttgctctaac agcatcactt tttgggtggc atccaaagaa tgcatgggat
     3241 tgagcattgg gtattgggcc agcacttgtg tggacacttg cgcaccgcgc atggcattgc
     3301 tctgcccttg tctggcaatc gctcgcaccg ttgacgcggc ttgcttcaag ctttctggca
     3361 cgatggaatc ggcataggca aaagcggttt tgtcaccaac aatggcgcgt atgccaaagc
     3421 cttcatcaat gttaaagctg ccgcttttca caatgccctc ttccaattgc caactttcaa
     3481 atgcggagtg ctggcaataa acatcagcaa aatccacttg atgctggcct acttctgcca
     3541 aaatgcggtc taagactgtt gcggaaatgc cgttgcgtgc caacaagtgc tcgctcgcat
     3601 gttgaaatac gtctggtgtg ctcattgttt gaaaccaaaa cctaaagaaa tatggtgtcg
     3661 actattgtag cggatttcat cgcacacaac gcaagccacg cacaataaac ttccactcaa
     3721 tctgaattac ctgccgtatt gagacttaaa tcgaaaacgg ctgcatatgc tggcttgagt
     3781 gcctttccaa tgcctctgtg tctcttcctt atttacaggc cgcatgtgaa gttttgttta
     3841 accaaatgtc agcattttga aattacttag attgatgaaa tattcattgc caccatccaa
     3901 acaccagcac ggtacccaga acagtatttc atttgaatgc tgatattagg tacaaagtca
     3961 tgcccagcct taagtcccta ctaaacgctc tgcgcccata caaacaagcc gcttgtcatc
     4021 cttggatcac aagcggcctg taaacacacc ttacctaatg gctactgagc acaagcgctg
     4081 cgttgcaaac gcccatatct gcccaaaacc tcattgattt tctgtgcatc ttgattggta
     4141 atgttgcgga acgacacagt gtattgcgca ctggtcaaag ctgattgcgc cgaacctcgc
     4201 tcagacacga tgacgccacc cacacctgcc gcaatggcac ggtctcgcac agaattggct
     4261 ttgcctgcat cagggtacac acccaaactg atgctgccac cttgtggact ggcactgatg
     4321 cccgcgctgc gcaattgttg taaagcctca tcactgctac ctgccaccaa aacttggtat
     4381 tgcacgccag atgccttgcc tttttgtttg ttttctacca cattgcgact ggcagcatgg
     4441 ttccaaccgc caataaaatt cttcaagcgg tggtaagcat cttcaggcaa ggtgatttgc
     4501 gcactgcaac tgggcgcatt tttgggcaaa gcagcggttt ccaaagtggt attgccacca
     4561 ccaccgcccg ccacagccgc aggcctgcgg gtaattgcgg gcgcaaccac gcgtgaaccc
     4621 gaggttgttc ccgcattttg cgccggtatc ggattgccac tggcatcagt gccgccacca
     4681 atgggcactg ccacaactgc tgggttttgt ttaggccgat tgccattcat tttttgtgtc
     4741 accacattgg cgaacacaat caaatttaaa accactaaaa ccgcaaacaa ccacttcaat
     4801 aattgcatgt gtgctctacc cagttttgca agccaaataa caccaaatta tccacaattt
     4861 tcacttggct gtctaagatt aaagcctttg gcaggtgttg ttcaattttc gaagcgccac
     4921 cacccgttaa aatcaaatca acaggttggc cttgttggcg ttcttttaag cgctggtgca
     4981 tcaaaatcac cgcaccgctg gccgcatcgt gcataccgct ggccaaggca ttcggtgtgg
     5041 aagtggcgta tggataagcc ttgcctatgg gctgattcaa attggcggtt ttggccgcca
     5101 tcgcctcttt catcaaatta aagcctggca tgatgctacc acccaaataa tggttgtcat
     5161 gggtgatggc atcgatggtg atggcggtgc cgcaagacac caccacacag gaattctgac
     5221 tgaacaggcg actgcccaag acgttaaacc aacggtctga gccatgttgc tgaactttgt
     5281 aatagtgatt ggtcacaccc aaagcatggc gcatagacgg cagccaacgc acgctgcgtg
     5341 tggcagcttc ttcaaccaaa gccatttttt tggcgccaca caccgcgcaa ccaatcaaag
     5401 gcaagtcttg tggctgcgca gccataaact ctgcaaagac ctgcaaattg gcatacggag
     5461 cccgcccacc aaagtgcaac tgctgctctg catccaccca agcccatttc aactggctgt
     5521 taccaccatc caataaaatg tatctcgcag cgcccgcttg tttacggtct ttgccctgat
     5581 tgctgacagg cttaggcatg acaaagtgtt catttgtggt tgtcttggct gtgactacgg
     5641 tttctagatg cgcggaggtg ttaaatagtg acacatcctg catgtctagt atgggctcag
     5701 gctctgcaac tggcattgct gacggtgtaa ccgtttgcgg cacatcgctc gcatgttcag
     5761 gccgcatgct gatttcgcca ttcagataca cttgctcttg cccagccgcg tcttgcacac
     5821 gcaagcctcc catgctgttg acacccaaca tctgtccttg cccgattaac tttcctcgct
     5881 caaacaagtt gactggttga ttcaaatcgc gcatttgtgc ggtgtaagcg gcctgaaaca
     5941 cctcaaaacc ctgtttgaag aaagtctgca tggcttggtc taattgcggt aaaagctgcg
     6001 ccaaaaaact ggtgcagctg aaatcggcgg tttgggcatt gcaagccgtg gcaatttcgc
     6061 gcaatgcctc ttgctgaggc tgcatcacat tgacaccgat gccaatgacc acatgccaac
     6121 gctcaccgcg ttgcttgctt tccaccaaga tgccgcacag cttgcgtgag tccaccacga
     6181 tgtcattggg ccatttcaac tgtgcatgca cttgctgctc tgccaacact tgttgcacca
     6241 acaagcccac caacaaaggc aaagccgcca ataccgcact gccttgctcc acttggtagg
     6301 ccaagctgaa catcagggtt tgtcccaaca cgttttgcca cggcctttgc aaacgaccgc
     6361 gcccttggct ttgcaccaag gcacacacca tgtgtttgtg tatgttgtac ccttcttgcc
     6421 agcgttgcca caacacatca ttggtagacg tggtttcagg caacacatcc acttgaaaac
     6481 ccgagctgtc gctgacagat gcggcctgta acggcgacac cactgccatc gcctgcttca
     6541 attgccacca accgtcttgc tgtcgcaagt gtttttgcac ttctgcaggc aattgtttcc
     6601 aatgcccgtt taaggcatgg gcatcacagc caagcagcgt ggccaaatcg cgcacatgtt
     6661 ggcgtttgcc atcggctaaa gcaccccaca aattccatgc atggtttgcc caacggttca
     6721 cgcttgcgcc tcacggattt tggccaaggt ttgcgtggta gaagtcgtgt gttcaaatgc
     6781 aatcgacacc actttaccgc cacgtgcttg ggtttctttc gcgcccacaa tgtgctctat
     6841 cgcccaatca ccgcctttga ccaacacttg tggctgtatc aattcaatca aattcgcagg
     6901 cgtgtcttca tcgaaccaag tcaccaaatc cacactttgc aaagccgcca ccacagccaa
     6961 gcggttttcc aagccattga tgggtctgtc atcgcccttg ccttggcgtt tcactgaggc
     7021 atcggtgttc aaagccaaca ccaagctgcc acccaaagcc cgtgcttggg ccaaatacgt
     7081 cacatggcca cggtgcaaaa tgtcaaaaca gccattggtg aacaccaacg gctgagccaa
     7141 ttgggcaatg cgggttgaca attcttctgg cgcacagatt ttgtgctcaa acactggggc
     7201 aggaaaatgc tgcatgagtt ctcttttctg tctgtgtaag cgacctgtaa acgatttcag
     7261 gccgcctcaa atccggtact ggcgtattgt gcaccattat ccatcacaat ggtacgctca
     7321 ggccaacgca aagcagcgag gtggaatttc gattgtgctg gtgcaatgtt ttgctggcgg
     7381 tcgcgccaac gcgctcctat tgaaaaatca atgcaaaaca cattgttttt ggcgccaaac
     7441 cacacgctgc catcctctgg catcaagcct gcatggcggg ttttggcggc agtatggttt
     7501 tgccacaagc gccaataatg ccccatcacc acaggcacat catcgtgata gtcctcccac
     7561 caagccgagc ggcctgtaaa acgccaacgc gcgcccgcat aaaacggctc actcacgggt
     7621 atttcactgc ccgataccaa tgcgcgcaca gggttttgtt tccagcgact caattcaaac
     7681 tcggtgtggc ccgacaacat tggtggatgt aaatcctctt tgcctaacaa ggcatcgtat
     7741 tgttggtatt cttgttggta ttgcgggtac caaggcttgt tgtgcaccaa ttcatgcaca
     7801 tgctcatcga aatagttgta ccactgggca atgttgcgct cagggtctag gccatggatg
     7861 gcattgatgg attcgggcaa ccaagccgcg tgcaccaaac gcaaatcctc gcgctgcaac
     7921 accaaaggcc aggtttgcaa atcggccaat acgccgctgc gctcatcaat tggcaaacgc
     7981 tgatacggca aatactgcgt gtcttcgggc atgcggctgt cgaaaaacca cgccgagcca
     8041 tctttggggt cgcccgccaa aatattcaat tcatgattgc ccaacacact caacacacca
     8101 tgctgctcac gcgcacgcat cacccaagcc aaggccgcag gcgaatcggg gccacggtca
     8161 acaatgtcgc caataaacac caagcgacgc ccttgcaggt gctcaccgcg ctcgtcgtaa
     8221 cccaaatgac gcaataattg ctgcaacacc gcccattcgc catgcacatc gccaacaatg
     8281 tcgag
//
LOCUS       DAOMVK010000039         8158 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_2291_length_8158_cov_3.181538, whole genome shotgun sequence.
ACCESSION   DAOMVK010000039 DAOMVK010000000
VERSION     DAOMVK010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 8158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8158
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2291_length_8158_cov_3.181538"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /gene="thiD"
                     /locus_tag="PK856_02425"
     CDS             <1..798
                     /gene="thiD"
                     /locus_tag="PK856_02425"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HRL01260.1"
                     /translation="GSDSGGGAGIQADLKTFQELNTFGTSAITALTAQNTLGVTGVHA
                     APPEFVLAQIDAVLADFDVQAIKTGMLFSAEIIEVVAKRMADYQGVLVVDPVMIAKGG
                     ASLLQQEAVAAMRTLLLPVATVIMPNIPEAEVLSGLKIETVADMAAAAQVLLDLGVKN
                     VVMKGGHSQEEMARDYVFLADGRRFSMATPRIDTPNTHGTGCTFSAALTAFVAQGLGL
                     PEAMVEAKRFIHLGIVHDLHLGHGHGPTNHFAYQQQKGECEVDCVWA"
     gene            773..1423
                     /gene="thiE"
                     /locus_tag="PK856_02430"
     CDS             773..1423
                     /gene="thiE"
                     /locus_tag="PK856_02430"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HRL01261.1"
                     /translation="MRWTVFGREQLKLYFIMGSQDMGGDLQAALNVLEQALKDGITLF
                     QWREKGAGSLAGQAAEYEQFARDCLALCRQYGVPFLVNDDVALALKLDAEGVHIGQDD
                     GDVAATRACIAHKILGVSAHSVEEVHSAAAAGADYVGLGPIYATVSKADANTPTGLVW
                     LEEARAAFPNLPIVAIGGINLERAPAVFAAGADGIAVISAICRKPSFVAEFQQLAL"
     gene            complement(1502..2599)
                     /locus_tag="PK856_02435"
     CDS             complement(1502..2599)
                     /locus_tag="PK856_02435"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRL01262.1"
                     /translation="MNYLAIALLLMLIMVVAELAYIQWVKRQAAPWADMVFNLNSGHV
                     LLWLFRGVELWVYGLVFHYANMHVIDGLPVWAQWLLAFIAWDFGFYCLHYFHHKSRLL
                     WAIHLVHHTGQDFNLSLGIRNSWYSSLSSIPFFWWLAVLGFTPTQFLMVSVAHYAIQF
                     YNHNSLYTQPTLLDKLLITPYSHRVHHAAQKPYLNTNFGSCFNVWDRLFGTYQTPQAD
                     VPMSYGAAFPHTHDPYVANQWPLQRLFGHTPKTQQNLQGKHAASPLLIASAGVALFLT
                     VIYYVLFEHQWQGLQVGVWVVGLTAATVAIGRYSDGHRSQAKLWCGLAVLLAAWVAWQ
                     GFGDGRWLVVAVLLAQVVLSLRLLRLPHLRY"
     gene            complement(2596..3258)
                     /locus_tag="PK856_02440"
     CDS             complement(2596..3258)
                     /locus_tag="PK856_02440"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRL01263.1"
                     /translation="MRTLWTYRCKQLFWAWAFVGCCYTLASLWPSSAPSIIQPSFVDD
                     AIGFQALWLPVYVSFFVFIPYGFFTLPHTVLPVLRTAFQVCGVLSALVFIAFPTTLVY
                     PDVSATSPSMGSWWAWWHQMDTPQNCLPSLHASLTTMVWLAVLRFAPQAACTQKAVMT
                     VWYGLILFSILACKRHFALDVAAGMALGGMCFAISLRGFNTCRSSASTEPDPLKPYKV
                     KT"
     gene            complement(3498..4361)
                     /locus_tag="PK856_02445"
     CDS             complement(3498..4361)
                     /locus_tag="PK856_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRL01264.1"
                     /translation="MKQHTLGWINGFIGVAIFSGTLPATRVAVQQLPPLFVTSARACI
                     AALLGGLLLCLLKQTRPHAAQLRSLILVSMGVVLGFPLFTSLALQHASSAHMIVFVGL
                     LPLSTALFAVWRGGERPKPLFWAFALVGAISVASYALLNSDGAPWFSDVLMLAAIVVC
                     GMGYAEGAKLSRELGGWQVISWALLLSLPVILPLALWSWPSPIAPVHSNAWWSLAYVS
                     VFSMLIGFVFWYQGLAQGGTAAVGQLQLLQPFMGLGLAALLLQEHIHPSMLVVTVVAV
                     LCVAGAKRFAH"
     gene            4480..5874
                     /locus_tag="PK856_02450"
     CDS             4480..5874
                     /locus_tag="PK856_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010327192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRL01265.1"
                     /translation="MATQQLTGRIGQVMAHVRTQIGNRTLVAGARLLSVRAQAKQLQV
                     SVSTVVEAYERLVAEGVILAKAGSGYYVLGSTAPLTLMAAGKPVDRAIDPLWISRQAL
                     EAEDQLLRPGCGWLPPQWLFEDGMRRALRAVARAPASSLSEYATPFGLPALRNLLARR
                     LSSTGLEVSPEQIMLTESGTQAIDLICRLFLQAGDTVLVDDPCYFNFHALLKAHRVKV
                     VSVAYTEHGPDLEAFQAALSEHKPRLYITNSGIHNPTGATLSPVFAHRLLKLIDGSDM
                     VVVEDDIYAEFELMPAARLSVFDGLERVIQIGSFSKTLSAAVRVGYVVAKPEWLDGLV
                     GLKIATSFSGGRLAQELVWHALTDSAYRKHLDAMRVRLAQAMQTVLPKLAAVGITPVF
                     MPLAGMFLWCRLPAGFDAAQVAQACLQQGVVLAPGNAFSLSQQAADCVRFNVAQCWDE
                     KIFAVLADVLKANA"
     gene            6029..6691
                     /locus_tag="PK856_02455"
                     /pseudo
     CDS             6029..6691
                     /locus_tag="PK856_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777701.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
     gene            6714..7859
                     /gene="hflX"
                     /locus_tag="PK856_02460"
     CDS             6714..7859
                     /gene="hflX"
                     /locus_tag="PK856_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRL01266.1"
                     /translation="MTQYKMDNSTVRPERIVLLAVDLTDFAGNRVVRQNDFQAAVAEA
                     KELIRATGGELVETVTCQKDRPSPALYIGSGKAEEIAELIETLEADLLVVNHPLTPTQ
                     ERNLEKACQCRVIDRVGLILDIFAQRARTQEGKLQVELAQLTHLSSRLVRGYAHLQSQ
                     KGGIGMKGPGETQLETDRRLIGNKITRLKRELQQVKKQRQTQRKSRYRNGTPTFALVG
                     YTNAGKSSVFNRLTKAEVLAKDQLFATLDTTARRLYLAPEVNVVITDTVGFVRDLPHE
                     LVEAFAATLEETAEADVLLHVVDRSHPEWSRQIDDVNEVLNQIGAEGIPCLLVLNKID
                     LLDEADALPAGAVLNAQGDVVAMHISVHNGAGLADLRQWMTEQAQSL"
     gene            7872..>8158
                     /locus_tag="PK856_02465"
     CDS             7872..>8158
                     /locus_tag="PK856_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRL01267.1"
                     /translation="MEQDDKQVTETVAETASDAVATQDEHKQKRLRDNAIYLLPNAFT
                     IAALFAAFQAIILATVHNEFEKAAFFIFASMILDGMDGRVARMTNSQSAFG"
BASE COUNT         2006 a         2031 c         2346 g         1775 t
ORIGIN      
        1 ggctcggatt cgggcggcgg cgcaggcata caggccgatt tgaagacgtt tcaagaattg
       61 aacacgtttg gcacatcggc gattaccgca ttgacagcgc aaaacacctt gggtgtgacg
      121 ggtgtacacg ctgcgccacc tgagtttgtg ctggcgcaaa tcgatgcggt gttggcagat
      181 tttgacgtgc aagccatcaa aacaggcatg ttgttttcgg ctgaaatcat cgaggtggtg
      241 gccaagcgca tggcggatta ccaaggcgtg ttggtggtag atccagtgat gatagccaaa
      301 ggtggcgcgt ctttgttgca acaagaagcc gtggcggcga tgcgcacgct gttattgcct
      361 gtggccactg tcatcatgcc gaatattccc gaggctgagg tgttaagcgg cctgaaaatt
      421 gaaaccgtgg ccgacatggc ggcggcggcg caagtgttgt tggatttggg cgtgaaaaat
      481 gtggtgatga agggcgggca cagccaagaa gagatggcac gtgattacgt gtttttggcc
      541 gatggtcgcc gtttcagcat ggcgacgccg cgcatagaca ccccgaatac ccacggtaca
      601 ggctgcacat tctccgcggc cttgaccgca tttgtggcgc aaggcttggg cttgcccgag
      661 gcaatggtgg aggcgaagcg ctttattcat ttgggcatcg tgcacgattt gcacttggga
      721 catggccatg ggccaaccaa ccattttgct taccaacaac aaaaaggcga atgtgaggtg
      781 gactgtgttt gggcgtgagc aattgaaact gtatttcatc atgggcagcc aagacatggg
      841 cggtgattta caggccgctt taaacgtgtt ggaacaagcc ttaaaagacg gcattacctt
      901 gtttcaatgg cgtgaaaaag gcgcgggcag tttggcaggg caggcggctg agtatgagca
      961 atttgcccgt gattgtttgg ccttgtgccg ccaatacggc gtgccttttt tggtgaacga
     1021 tgacgttgct ttggctttga aactcgatgc cgaaggcgtg cacattggtc aagacgatgg
     1081 cgatgtggcg gccacgcggg cgtgcatagc ccataaaatc ttgggcgtgt cggcacacag
     1141 cgtggaagaa gtgcacagcg cggcagcggc gggcgcggat tatgtgggct tggggccgat
     1201 ttatgccacg gtttccaaag ccgatgccaa cacaccgacg ggcttggtgt ggttggaaga
     1261 ggcgcgagcg gcgtttccca atttgcccat cgtggccatc ggtggcatca atttggaacg
     1321 tgcgcctgcg gtattcgcag cgggggccga tggcattgcg gtgatttctg cgatatgccg
     1381 caagccttca tttgtggctg aatttcagca attggcgctg taaatacagc atttgcaatc
     1441 tctatcatcc aagcggcctg taagtgttta caggctgctt gtttgtgtat gtgggttaca
     1501 ttcaataacg caggtgtggc agcctgagca aacgcagcga caacaccact tgtgcaagca
     1561 ataccgccac caccagccag cgcccgtcgc caaacccttg ccaagccacc catgccgcca
     1621 gcaataccgc caagccacac cacagcttgg cttggctgcg atggccatcg ctgtagcgac
     1681 cgatggcaac cgtggccgcc gtcaatccca ccacccacac gcccacttgc agcccttgcc
     1741 attggtgttc aaacaacacg tagtaaatca cagtgagaaa caaggccacg cctgcgctgg
     1801 caatcagtaa aggcgaagcg gcgtgtttac cctgcaagtt ttgctgtgtc ttgggtgtgt
     1861 gtccgaacaa gcgttgcagc ggccattggt tggcaacata agggtcgtgg gtgtgtggaa
     1921 atgcggcgcc atacgacatg ggtacatcgg cttgcggcgt ttggtaagtg ccaaacagcc
     1981 tgtcccacac attgaagcag ctgccaaaat tggtgttcag atacggcttt tgcgcggcgt
     2041 ggtgaacgcg gtggctgtaa ggcgtaatca agagtttgtc cagcaaagtc ggttgggtat
     2101 aaaggctgtt gtggttgtaa aactggatgg cgtaatgcgc caccgatacc atcaaaaact
     2161 gggtgggcgt gaagcccaat accgccagcc accagaaaaa tggaatcgag ctgagggagg
     2221 aataccacga attgcgtatg cccaaagaca ggttgaaatc ttgaccggtg tggtgcacca
     2281 agtgtatcgc ccacagcagg cgcgatttgt ggtggaagta atgcaggcaa taaaagccaa
     2341 aatcccaagc gataaaggcg agcaaccact gcgcccacac aggcagaccg tctatcacat
     2401 gcatgttggc atagtgaaac accaagccat acacccacaa ttccacgcca cgaaacagcc
     2461 acagcaacac atggccagaa ttgaggttga ataccatgtc tgcccatggt gcagcttggc
     2521 gcttgaccca ttggatgtag gccaattcgg ccaccaccat gatgagcatc agcaataagg
     2581 cgatggcaag ataattcatg ttttcacctt gtagggtttg agtgggtcgg gctcggttga
     2641 ggcagagctg cggcaggtat taaacccacg caggctgatg gcaaaacaca ttccacccaa
     2701 tgccatgccc gcggccacat ccagtgcaaa atggcgtttg cacgccaaaa tgctgaacaa
     2761 aatcagccca taccacaccg tcatcacagc cttttgtgta caggccgctt gtggagcaaa
     2821 acgcaataca gccaaccaca ccatggtggt caaactcgcg tgcagcgagg gcaggcagtt
     2881 ttgtggcgtg tccatttgat gccaccaagc ccaccatgac cccatacttg gactggtggc
     2941 actgacatcg gggtaaacca aggtggtagg gaaggcaatg aagaccaagg ccgacaagac
     3001 gccacagact tgaaatgcag tgcgcaatac aggtaacaca gtgtgcggca gcgtgaaaaa
     3061 accataagga atgaacacaa aaaacgacac atacacaggc agccacagcg cttggaagcc
     3121 aattgcatcg tctacaaacg agggttgaat aatgctaggg gcgctgctgg gccagagcga
     3181 ggctaaggtg tagcaacagc ccacaaaagc ccaagcccaa aacaattgct tacagcggta
     3241 agtccacagg gtgcgcatgg tgttagcgtt cagtgtcgtg atggtcgatg gtcagaggtg
     3301 tagcaagagc cataccttgg tggcaagatt tggcgcatgg ggtggtggtg tttgctgctt
     3361 ggggttttat gcggcgaacc taaaaccatt atttgaggag cgtgggttga acactgatat
     3421 caggattggt aagggataag cggcctgtaa aaaacttaca ggccgcttat tcagaaacac
     3481 tttgttgtaa ttaatattta gtgagcaaag cgcttggcac cggccacaca caataccgcc
     3541 accactgtca ccactaacat gctggggtgg atgtgttctt gcagcagcaa ggccgccaaa
     3601 cccaagccca taaatggctg taacaattgc aattgaccca cggcggccgt accaccttgc
     3661 gccaaacctt gataccaaaa tacaaatcct atcaacatgc tgaacaccga tacataggcc
     3721 aagctccacc aagcgttgct gtgtacgggc gcgatgggcg agggccaaga ccacaatgcc
     3781 aaaggcaaga tgacaggcaa agagagcagc aatgcccatg aaatcacttg ccaaccaccg
     3841 agttcacgcg acagttttgc gccctcggca tagcccatgc cgcacaccac aatggctgcc
     3901 aacatcaaca catcgctgaa ccacggggca ccatcgctgt tgagtaaggc atacgaagcc
     3961 acactgatgg cgccgaccaa ggcaaatgcc caaaacagcg gcttgggccg ttcacccccg
     4021 cgccacacgg caaacaaggc tgtgctcaaa ggcagcaagc ccacaaacac aatcatgtgt
     4081 gccgaggatg cgtgttgtaa ggccaaagag gtgaacaagg ggaaacccaa aaccacgccc
     4141 atgctgacca agatgagcga gcgcagctgc gcggcatggg gccgggtctg tttgagcaaa
     4201 cacaagagca agccgccaag caatgccgcg atacaggcgc gggcgctggt gacaaacaaa
     4261 gggggcaatt gttgtaccgc cacgcgggtg gctggcaaag tgcctgaaaa aatggccacg
     4321 ccgatgaaac cattaatcca gcccaaggtg tgttgcttca ttgttgactc ctgttgttga
     4381 tgtgcacagt aaaccatgca cagtggcggc aaaacagaga caatccagta caattgcaaa
     4441 gaactgtcat ggtatttgag caatacaatt ggaggtgaaa tggcaacgca acagcttaca
     4501 ggccgcatag ggcaggtgat ggcgcatgtg cgcacacaaa ttggcaatcg cacgctggtg
     4561 gcgggggcgc gtttgctttc ggtgcgggcg caggccaagc aattacaggt atcggtgtcg
     4621 accgtggtgg aggcgtatga gcgcttggtg gctgagggag tgatattggc caaggcgggt
     4681 tcaggctatt atgtgttggg cagtacggcg cccttgacct taatggcagc aggcaaacct
     4741 gtggacaggg ccatagaccc tttgtggatt tcgcggcagg ctttggaagc ggaagaccag
     4801 ttattgcgac caggttgtgg ttggttgccg ccacaatggc tgtttgaaga cggcatgcgc
     4861 cgcgctttgc gggcggtggc ccgcgcacct gccagcagtt tgagcgagta tgccacgcct
     4921 tttggtttgc cagctttgcg caatttgctg gccagacgtt taagcagtac aggcttggaa
     4981 gtgtcgcccg agcaaatcat gttgacggaa tcgggcacgc aagccatcga tttaatctgc
     5041 cgcttgttct tgcaagcggg cgatacggtg ttggtagacg atccgtgtta tttcaatttt
     5101 catgctttgc tcaaagcgca cagggttaaa gtggtcagtg tggcctatac cgagcatggc
     5161 cctgatttag aggcttttca ggccgccttg agtgaacaca aaccgcgttt gtacatcacc
     5221 aattcgggca tacacaatcc aacaggcgcg actttgtcgc ctgtgtttgc ccaccgttta
     5281 ctcaagttga tagacggcag cgatatggtg gtggtggaag acgacattta cgccgaattt
     5341 gaactcatgc ctgcggcccg attgagcgtg tttgatggct tggagcgggt catccaaatc
     5401 ggcagctttt ccaaaacctt gtcggcggcg gtgcgcgtgg gctatgtggt ggccaaaccc
     5461 gagtggctgg atggcttggt gggtttgaaa atcgctacca gtttttcagg tggccgattg
     5521 gcgcaagaat tggtgtggca tgccttaaca gacagcgctt atcgcaaaca cttggatgca
     5581 atgcgcgtgc gtttggcgca agccatgcaa actgtgttac ccaaattggc ggcggtgggc
     5641 atcactcctg tgttcatgcc tttagcggga atgtttttgt ggtgtcgcct gcctgcaggc
     5701 tttgatgctg cgcaagttgc gcaagcgtgt ttgcagcaag gcgtggtgtt ggcgccaggc
     5761 aatgccttta gcttgtcgca acaagccgcc gattgtgtgc gcttcaatgt ggcgcagtgt
     5821 tgggatgaga aaatatttgc ggtattggcg gatgtgttga aagccaatgc ttgagccatg
     5881 tgtggacttg gcttacaggc cgattgagaa gaatgatgaa aagccgcgta aacaagcggc
     5941 ctgtaaagcg ctcagccatt attggcagca gtttgttgaa tgcggttata atggcgaact
     6001 taattgaccg agtatccaag gagccgctgt ggccaaagca cattatccgc acccgatttt
     6061 agcccgcgaa ggctggccat tcatcatagt taccgtgttg gcaagtttgt tgattacctg
     6121 cctgtttggc gcgtggtctt tgccattttg gttgattacc gcttttgtgg tgcaattttt
     6181 ccgcgaccct gcgcgtgaaa ccccaaacaa tgatgccaac gccatcacca gccccgctga
     6241 tggtcgcata gtggttatcg aaaaaaccca agacccttat cgccattgcg aagccttgaa
     6301 aatcagcgtg ttcatgaatg tgtttaacgt gcattctaac cgtgcaccga ttgcaggtac
     6361 ggtagagcgc gtggaatatt ttgctggctc ttttgttaat gccgatttgg acaaagccag
     6421 tttggaaaac gagcgcaatg cggtggtgat gaaaaccgac agcggccacg acatcacgtt
     6481 tgtgcaagtg gcaggcttgg tggcgcgtcg catcttgtgt tacaccaaag tgagcgacca
     6541 tttgtaccgc ggtcaacgct atggcttcat ccgctttggc tcacgcgttg atttgtattt
     6601 gcccgtggac tcacaagcac aagtgaccat cggtcaaaaa gtgtcagctt ccaccaccat
     6661 cttggcacgt ttgcctgacc aaaacgctga ataagcggcc tgaaaaaccc acaatgacac
     6721 aatataaaat ggataacagc acggtgcgcc cagagcgcat cgtgcttttg gctgtggatt
     6781 taaccgactt cgcgggcaac cgcgtggtgc gccaaaacga ttttcaagca gcggtggccg
     6841 aagccaaaga attgatacgt gcgacaggcg gcgaattggt cgaaaccgtg acctgccaaa
     6901 aagaccgacc ttcacctgct ttgtacatcg gcagtggtaa ggccgaagaa atcgccgaat
     6961 tgatcgaaac gctggaagca gatttgttgg tggtaaacca tccgttgacg cccacgcaag
     7021 agcgcaattt ggaaaaagcc tgccaatgcc gtgtcatcga ccgcgtgggt ttgattttgg
     7081 acatctttgc ccaacgcgcc cgcacgcaag agggcaagct gcaagtagag ttggcacagc
     7141 tcacccactt atccagccgc ttggtgcgtg gctatgcgca tttgcaaagc caaaaaggcg
     7201 gcataggcat gaaaggccca ggggaaaccc agctggaaac cgaccgccgc ttaattggca
     7261 acaaaatcac ccgcctcaag cgcgaattgc agcaggtgaa aaaacagcgc caaacccagc
     7321 gcaaaagccg ctaccgcaat ggcacgccca cattcgcttt ggtcggctac accaatgcag
     7381 gcaaatccag tgtgttcaac cgcttgacca aggccgaagt attggccaaa gaccaattgt
     7441 ttgccacact cgacaccacc gcgcgccgct tgtatttggc gcccgaagtg aacgtggtca
     7501 tcaccgatac cgtcggtttt gtgcgcgatt tgccgcatga gttggtggag gcttttgccg
     7561 ctactttgga agaaaccgcc gaagccgatg tgttgttgca tgtggtcgat cgctcgcatc
     7621 ccgaatggtc gcgtcaaatt gatgacgtga acgaggtgtt gaaccaaatc ggggcggaag
     7681 gcatcccatg tttgttggta ttgaacaaaa ttgatttgtt ggatgaagcc gatgccttgc
     7741 ctgcgggcgc ggtgttgaat gcccaaggcg acgtggtggc gatgcacata tcggtgcata
     7801 atggtgctgg cttggcggat ttgcgtcaat ggatgactga gcaagcacaa tctttataga
     7861 ggacaagcag catggagcaa gacgacaagc aagtgacaga aactgtggct gaaaccgctt
     7921 ctgacgctgt cgcaacacaa gacgaacaca agcaaaaacg cttgcgtgac aatgcgattt
     7981 atttgttgcc caatgcgttc acgatagcgg ctttgtttgc cgcgtttcaa gcgattattt
     8041 tggcaacggt acacaatgag tttgaaaaag ccgcgttttt catcttcgcc tccatgattt
     8101 tggatggcat ggacggccgc gtggcgcgca tgaccaacag ccaaagcgcg tttggtga
//
LOCUS       DAOMVK010000040         8071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_2321_length_8071_cov_3.079466, whole genome shotgun sequence.
ACCESSION   DAOMVK010000040 DAOMVK010000000
VERSION     DAOMVK010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 8071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8071
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2321_length_8071_cov_3.079466"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..1266
                     /locus_tag="PK856_02470"
     CDS             70..1266
                     /locus_tag="PK856_02470"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HRL01268.1"
                     /translation="MFEHVDYYPGDPILGLNDLYNKDTRAEKVNLGVGVYYDEEGRLP
                     LLGSVLKAEEARAKAPSARPYLPMDGLPAYNKAVQRLLFGADSAAVAADRVATIQSIG
                     GSGALRVGAEFIKQYFPQSKVYVSKPTWGNHISIFEATGLEVGEYPYYDAATGGVAFD
                     ALIEKFNSLNKHDVVLLHPCCHNPTGVDLTPAQWDKVIAVVQARELIAFMDIAYQGFG
                     DDFETDVYAIRAAEKAGLSYFVSSSFSKNLSFYGERLGALSVVSPNAEEKKLVLSQLK
                     YIVRRLYSNPPCHPAIIAADVLNDEVLFKEWTDEVAEMRVRIKAMRQRLFEVLSAKVP
                     GRDFSYITTQRGMFSFSGLSPAQVERLINDYGIYLVKNGRICVAGLNTKNIEYVANAF
                     ADVLKD"
     gene            complement(1330..1755)
                     /locus_tag="PK856_02475"
     CDS             complement(1330..1755)
                     /locus_tag="PK856_02475"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HRL01269.1"
                     /translation="MDNSEAVRKHQQIMSQQALFERHVQALKKNLSDIEFIVVASDDG
                     FPITHTLADDKDAARKAAMTASLGGLSTSVSEESGLKGAMATTIECEDGLVFSRHVML
                     DHEKSVELLVACTRHANYATALWTIKKTINDIIEDFNRR"
     gene            complement(1759..2319)
                     /locus_tag="PK856_02480"
     CDS             complement(1759..2319)
                     /locus_tag="PK856_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HRL01270.1"
                     /translation="MSVSNDTAKSAPLLKILFIGPMGAGKTTAIRCISDSDPIGTEAS
                     NSDSGQSTKSTTTVAMDYGSIELGPEEVVHLYGIPGQDRFKFMWPILAKGALGCILMI
                     DDTRADPMADLTLYLHEFNDLVSKQAFVVGVGKTNKDGSSLEKYNQYFAERDMYAPIM
                     EVDARDKADVLMLLEVILANTEIKGN"
     gene            complement(2339..3988)
                     /locus_tag="PK856_02485"
     CDS             complement(2339..3988)
                     /locus_tag="PK856_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01271.1"
                     /translation="MSDVENFNVNMNLVGFGLDATEKFTKQIDTINRMNAAQDRLKYV
                     WGVGKQIQNCQVHFVISSPLPAYLENTADPVLTNQNIETDNIIPCPFPVTTFDLMDIL
                     AYVESLIPESRAQSNSTNTSQTEAHEIETDNQQDTPVDFSSMSLYERARLGLNNKLSQ
                     IEATSPAATSNAEEADLNTGNKTDFTFKSYQATPQPTEAPKKGTFTTYGSFSMEGSVH
                     KNTSSEPSVEATEKPVVSSWTNTYTNTPKKTEPIEPPSSTSAPTTEVKQHKTETFIRY
                     GSLKNNVADEPAKPTPSISDEIKITTAPTNAEAPTPNFDTTTAEENTDLSAQALSEEK
                     NHTASKQSSTSEAASYTDIWLDFLAYQQSLIHVKEPEFYCDAEQKNISSNYKNASELA
                     QGMIKQTEIVSDTVNNIPNDLKKLPLSAVLWSYGLHHPHAEQLLQAMDIENHEWKLKG
                     FPRFGLWETHPTWLFLATFFAQKHQSIFAAQAKGKADATQIINFICAAKLAGLTWDSR
                     PLATELKQQQMAEVSEYPHKESASWISRLRSKLHINEHFES"
     gene            complement(4075..4452)
                     /locus_tag="PK856_02490"
     CDS             complement(4075..4452)
                     /locus_tag="PK856_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003785259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01272.1"
                     /translation="MQRKGFTTNFDKTISELLSVDGALAAAVVDYSSGMLLAGGGSSN
                     VDLEIAAAGNTEVMRAKMKTMGMLGLNDIIDDILITLGEQYHLLRPLQKHDGLFLYHV
                     LDKSKSNLALARRALREAEKNLV"
     gene            complement(4708..5490)
                     /gene="cysE"
                     /locus_tag="PK856_02495"
     CDS             complement(4708..5490)
                     /gene="cysE"
                     /locus_tag="PK856_02495"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HRL01273.1"
                     /translation="MTAALSTEQVWQTILAETVEAADHEPTLASFLHLTVLRHSSLNR
                     VLAFHLSSKLASPVMDARTLYEMFHHALVSDQNIHDAAIADIVAYYERDPACDAYSLP
                     LLYFKGYHAIQAHRINHYLWLQGRKTLAYFLQNRASEVFGVDIHPAAVFGKGLMIDHG
                     TGIVIGETAVLGDNISLLHGVTLGGSGKESGDRHPKISDGVMIGANASVLGNIRINQC
                     AKVGAGSVVVEDVPAYTTVVGVPAREVGKTGSIPAQEMDQRL"
     gene            complement(5508..7004)
                     /gene="rng"
                     /locus_tag="PK856_02500"
     CDS             complement(5508..7004)
                     /gene="rng"
                     /locus_tag="PK856_02500"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HRL01274.1"
                     /translation="MLQNAIALAKDVPRPPETVLVNVTPQETRVAILENSTICELHIE
                     RNSGRGLVGNIYLGVVKRVLPGMQSAFIDIGLDRAAFLHIVDILEQRQHPTETQRIEH
                     MLFEGQAVLVQVIKDPINSKGARLSTQISLAGRFLVPLPQDEHIGISQRIEDESERAH
                     LRSRLEALLPQESCHGYIIRTSAETATDQELQADIDYLSRLWENIRKRSKIAPPETLL
                     YQDLPLSRRVLRDMFSDNTQRILVDSNENFESMLEFAQMYVNNAISKIEKFDGARPLF
                     ETYGVEEEIDKALHPRVNLKFGGYLIIDPTEAMTTIDVNTGGFVGTRNFDETIFKTNL
                     EACHAIARELRLRNIGGIVIVDFIDMEQEQHREAVLNELTRVLSLDRTRVTLNGFTSL
                     GLIELTRKRTRESLNHLLSEPCPCCNGRGTLKTPQTVCYEIQREIVGEARRYPAKSYK
                     ILASQAVIDLLLDEESQSLAMLIDFIGAPITLAVETSYNQEQFDIILM"
     gene            complement(7519..>8071)
                     /locus_tag="PK856_02505"
     CDS             complement(7519..>8071)
                     /locus_tag="PK856_02505"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HRL01275.1"
                     /translation="AQLLAQIGLHITKIASDIDETPHENELALAYTERMAWQKSEQAL
                     ALFLASHGAKPELPVLTADTSVAIDGLILGKPDNHAHAVTMLRMLSGRSHQVISSVVM
                     WHQGQRSQATQVSDVTFRPLTEAEIQAYVDSGEADDKAGAYGIQGAAAVFIEHLSGSF
                     SGVMGLPLFETAALLRQLNVSKQ"
BASE COUNT         2023 a         1728 c         1974 g         2346 t
ORIGIN      
        1 gatgggtgca ggttgcctta caacgacaac ttggactgtt ttaatctgca actttaggac
       61 aaaaacctca tgtttgaaca tgttgattat tacccaggtg acccgatttt gggtttgaat
      121 gacttgtaca acaaagacac acgtgctgag aaagtaaact tgggcgtggg tgtttattac
      181 gatgaagaag gtcgcttgcc tttgttgggc tcggtgttga aagcggaaga ggcacgtgcc
      241 aaagccccat cagctcgccc atatttgcct atggatggct tgccagccta taacaaagcg
      301 gtgcaacgct tgttgttcgg tgctgacagc gccgcagttg ccgctgatcg cgttgccacc
      361 attcagtcta tcggtggctc aggtgctttg cgtgtgggtg ctgaattcat caaacaatac
      421 ttcccacaaa gtaaagtgta tgtgagcaaa cccacttggg gcaatcacat cagtattttt
      481 gaagcgaccg gcttggaagt gggtgagtac ccgtattacg atgcagccac tggtggcgtt
      541 gcattcgatg ccttgattga aaaattcaac agcttaaata agcatgatgt ggtgttgtta
      601 cacccttgct gtcacaatcc aacgggtgtg gatttgacgc ctgcacaatg ggacaaagta
      661 atcgccgtgg tgcaagcgcg tgaattgatt gcgttcatgg acattgctta ccaaggtttt
      721 ggcgacgatt ttgaaaccga tgtatacgca atccgtgcgg ctgaaaaagc gggcttgagt
      781 tattttgtca gcagttcttt ctctaaaaac ctgtcattct acggcgaacg cttgggtgct
      841 ttgtctgtgg tgtctccaaa cgcggaagag aaaaaattgg tgttgagcca attgaaatat
      901 atcgtgcgcc gtttgtattc taacccacct tgccacccag ccatcatcgc tgccgatgtg
      961 ttgaacgacg aagtcttgtt caaagagtgg acggatgaag tggccgaaat gcgtgtacgc
     1021 atcaaagcca tgcgccaacg tttgtttgag gtgttgtcag ccaaagtacc tggccgtgat
     1081 ttcagctaca tcaccactca acgtggtatg ttcagcttct ctggcttgag ccctgcgcaa
     1141 gtggagcgct taatcaatga ctacggcatt tacttggtga aaaacggtcg catttgtgtg
     1201 gctggcttga acaccaaaaa cattgaatac gtggccaatg cgtttgccga tgtgttgaaa
     1261 gactgagtat taaagtattg aaagcggcct gtaaatcatt tacaggccgc ttttttatgc
     1321 ttttaaacct tatctgcggt taaagtcttc aatgatgtca ttgatggttt tcttaattgt
     1381 ccacaaggca gtggcgtaat tggcatggcg ggtacaggcc accaacaatt ctacggattt
     1441 ctcatgatcg agcatgacat ggcgactgaa aaccaagcca tcttcacatt ctatggtggt
     1501 tgccatggcg cctttgaggc cagactcttc cgatactgaa gtactcaaac cacccaaaga
     1561 ggcggtcatg gccgcttttc gagcggcatc tttgtcatct gccaaagtat gggtgatggg
     1621 aaaaccatca tcgctggcaa cgacaatgaa ttcaatgtcg ctgaggtttt ttttcaaagc
     1681 ttgcacatgg cgctcaaata aggcttgttg cgacatgatt tgttgatgct tgcggactgc
     1741 ttcgctgtta tccattgctt aattcccttt gatttctgtg ttcgctaaaa tgacttccaa
     1801 tagcatcaat acatcggctt tgtctcgcgc atcgacttcc atgatggggg cgtacatgtc
     1861 gcgctctgca aagtattggt tgtatttttc caatgatgag ccatctttat tggttttgcc
     1921 cactccgacc acgaaagcct gcttggagac caagtcattg aattcatgta agtaaagtgt
     1981 caggtcggcc attgggtctg cgcgagtatc gtctatcatc aagatacaac ccaaagcccc
     2041 tttggccaaa atcggccaca tgaatttgaa acggtcttgg cctggaatgc catataagtg
     2101 caccacttct tcaggtccca gttcaatact gccgtaatcc attgccacag tggtggtgga
     2161 tttggtggat tggcctgaat cagagttgct ggcttctgtg ccgatggggt cgctgtcact
     2221 gatgcagcgg atggctgtgg ttttacctgc ccccatgggg ccaataaaca agattttcaa
     2281 cagcggtgct gatttcgcag tgtcgttgct tacagacatg atggagcttt ccaattaatt
     2341 atgattcaaa atgttcatta atatgcagtt tgctgcgcaa gcgggaaatc caagaggcac
     2401 tttctttgtg cgggtattcg gatacttccg ccatttgttg ttgcttgagt tcagtcgcaa
     2461 gagggcggct atcccatgta agtccagcca gttttgcagc gcaaataaaa ttgatgattt
     2521 gtgtggcatc ggctttgcct ttggcttgcg cggcaaaaat actttggtgt ttttgggcaa
     2581 aaaatgtcgc caaaaacaac caagtgggat gggtttccca taagccaaaa cgtgggaagc
     2641 cttttagctt ccattcgtga ttttcaatat ccatggcttg aagtagctgc tcagcatggg
     2701 gatggtgtaa gccataagac cataagactg ctgacaatgg caactttttt aagtcgtttg
     2761 gaatattatt taccgtgtct gacacaattt cggtttgttt aatcatgcct tgtgccaatt
     2821 cagaggcatt tttgtagttg ctgcttatat ttttttgctc ggcatcacag taaaactctg
     2881 gttctttaac gtgtatcaag ctttgttgat acgctaagaa gtctagccaa atatcggtat
     2941 aggaagcagc ttcagaggtt gagctctgtt tacttgccgt gtggtttttt tcttcagaca
     3001 atgcttgagc acttaaatca gtattttctt ctgctgtggt tgtatcgaaa ttgggtgttg
     3061 gcgcttctgc gtttgttgga gcagttgtga tttttatttc atctgagatc gaaggtgttg
     3121 gttttgctgg ctcatcggcc acattgtttt ttaagctgcc atagcggatg aaggtttcag
     3181 ttttgtgttg cttgacttca gtggtgggag ctgacgtgct tgagggcggt tcaatgggtt
     3241 cggttttctt gggcgtattg gtataggtat tggtccaaga gctgaccaca ggtttttcag
     3301 tagcttcaac actgggttca gagcttgtat ttttgtggac actgccttcc atgctgaagc
     3361 taccatacgt ggtgaatgtg ccttttttag gtgcttcggt gggttgtggt gtcgcttgat
     3421 acgatttaaa agtaaagtca gttttgttgc cagtgtttaa atctgcttct tcagcatttg
     3481 aggtggcggc agggcttgtg gcttcaattt ggctgagttt gttgttaagt cctagacgtg
     3541 ctcgttcata cagcgacata gaggagaaat ccacaggagt atcctgttgg ttatctgttt
     3601 ctatttcgtg tgcttctgtt tgagaagtgt tggtggaatt ggattgagct cggctttcag
     3661 gaatgagaga ttccacatag gccaaaatat ccattaaatc aaatgtggtg acagggaaag
     3721 ggcacggaat aatgttgtcg gtttcaatgt tttgattggt taaaactggg tctgcagtgt
     3781 tttccaaata agcaggcaaa ggagagctga tgacaaaatg cacttggcaa ttttgaattt
     3841 gcttaccaac accccaaaca tatttgaggc ggtcttgtgc cgcattcatg cggttaatgg
     3901 tgtctatttg cttggtgaat ttttctgtgg cgtccagtcc gaaacccacc aagttcatat
     3961 tcacattgaa attttcaaca tccgacattt taactcgctc aagtagaatc gcctcggcaa
     4021 taacgtatta ccgaggcgtg tgtgcatacc tatcaaactg gtgacgcgaa acggttatac
     4081 caagtttttc tcagcttcac gcaaggcgcg acgagccaaa gccaagttag atttgctctt
     4141 atccaacaca tggtataaaa ataaaccatc gtgtttttgt aatggacgca ataagtggta
     4201 ttgctcaccc aaagtaatca agatgtcgtc gatgatgtca ttcaagccca gcatgcccat
     4261 ggttttcatt ttggcgcgca tcacttcagt attgcctgct gccgcaattt ccaagtccac
     4321 attagaagag ccgccaccag cgagcaacat gccgcttgag tagtctacaa ctgctgctgc
     4381 taacgcaccg tctacactta ataactcaga gatggtttta tcaaagttcg tggtaaatcc
     4441 ttttcgttgc acgaaattat ttaaaaaaat ttaatacagg tgatgcaatg aaagtgcagt
     4501 tgatgttgac ggtttttaat tccagttttg atctgcttga aagcagcttg aaattaataa
     4561 gcttcaaaac cgcgcgcaat catcatatca cactaaaagt aactttcaat ctatgtgaaa
     4621 cagattctat gtgataaata tctatttgac gattgaattt ttttactaca aaggcataaa
     4681 ggtttttaag gatttatcag cttaaaatca aagacgttga tccatttctt gtgctggaat
     4741 actgccagtt ttgcccactt ctcttgccgg cactccaacc actgtggtgt aagcaggaac
     4801 gtcttcaact acaacgctgc cagctcccac ttttgcacac tggttaatgc ggatattgcc
     4861 caataccgat gcattggcac ctatcattac accatcactg attttaggat gtcggtcgcc
     4921 tgattctttg cctgaaccac ctaatgtaac accatgcagt aaggagatat tgtcacccaa
     4981 tacggcggtt tcaccaatga cgatgcctgt accatggtct atcatcaaac ccttaccaaa
     5041 tactgctgca ggatggatgt ctaccccaaa aacctctgag gctctgttct gcaaaaagta
     5101 tgccaatgtt tttcgccctt gtagccacaa atagtggtta attcgatggg cttggatggc
     5161 gtgatagcct ttaaaataaa gtaaaggcag tgaatatgca tcacatgcag ggtcgcgttc
     5221 ataataagcc acgatgtcag caatcgcagc atcatgaatg ttttggtcag acaccaaagc
     5281 gtgatgaaac atttcataca aagtacgtgc atccatgaca gggctggcca atttgctgga
     5341 caaatggaaa gccaagaccc gattcagcga tgagtgacga agcacggtca agtgcaaaaa
     5401 gctcgcgaga gtcggttcgt ggtctgcggc ttcaacagtt tccgccaaaa tggtttgcca
     5461 gacttgttct gtgctcagtg ctgcggtcaa aatacttgct ccagaaatta catcaaaata
     5521 atatcaaatt gttcttggtt gtatgaggtt tccacggcca gtgtgatcgg tgcgccaata
     5581 aaatcaatga gcatggccaa agattgggat tcttcatcca ataacaaatc tatcactgcc
     5641 tgagaagcca atattttata gcttttggca gggtagcgcc gtgcttcacc aacgatttca
     5701 cgctgaattt cataacacac agtctgaggt gtttttaaag tgcctcggcc attgcagcat
     5761 gggcaaggct cactcagtaa gtggttcaaa ctttcacgtg tacgcttgcg agtcaattca
     5821 atcaaaccca agctggtaaa gccatttaag gtgacgcgtg tgcgatccaa acttaaaaca
     5881 cgggtcagct catttaaaac agcctcacgg tgttgttctt gctccatgtc gatgaaatcg
     5941 acgatgacaa tgccaccaat attgcgcaag cgcagttcac gcgcgatggc atgacaagcc
     6001 tctaaattgg ttttgaaaat ggtttcatca aagttacgtg tgcccacaaa accgcctgta
     6061 ttgacatcta tggtggtcat ggcttccgtg gggtctatga tgaggtagcc accaaacttc
     6121 aaattcacac gagggtgtaa ggctttgtcg atttcttctt caacgccata agtctcaaac
     6181 aaagggcgcg cgccatcgaa tttttcgatt ttgctgatgg cattgttcac atacatttgc
     6241 gcaaattcga gcatggattc aaagttctca ttggaatcca ccaaaatgcg ttgtgtattg
     6301 tcactgaaca tgtcacgcaa tacccgtctg ctcaaaggca aatcttggta taacaaggtt
     6361 tcaggagggg cgattttgct gcgcttgcgg atgttttccc ataagcgcga caggtaatca
     6421 atatcggcct gtaactcttg gtcagtagcg gtttcggcac tggttctgat gatgtagcca
     6481 tgacacgatt cttgtggcaa taaggcttcg aggcggctgc gtaaatgagc acgttcgctt
     6541 tcatcctcga ttctttgtga aatgcctatg tgttcatctt gtggcagggg gaccaaaaag
     6601 cgcccagcca aagagatttg ggtggaaagt cgtgcgcctt tgctgttaat ggggtctttg
     6661 atgacttgca ccagcaccgc ctgtccttca aacaacatgt gttcgatgcg ctgagtttca
     6721 gtagggtgtt ggcgctgttc taagatgtcg acgatgtgta gaaaggcggc tctgtccaaa
     6781 ccaatgtcga tgaacgcgct ttgcatgcca ggtaagaccc gtttgaccac acccaaataa
     6841 atattgccga ccaagccacg gcctgaattg cgctcaatgt gcaattcaca aatggtggag
     6901 ttttccaaaa tggcaacacg ggtttcttgg ggggtgacgt tgacgagaac cgtttcaggt
     6961 gggcgcggca catcttttgc caaggcaatg gcattttgta acatgatgca tcctttgatt
     7021 tcaatgtggt ttgttcagta ggcgccaacc atgcctgctg agcaaacaac actaccgcta
     7081 aaagcgcttt atcttaacac aagcacatgc tgcgagagta gtcagcattg tttagtaatg
     7141 gtgcagattg acagttttac aaggtggtgg attttaaagt gctgaaatta caattaaatc
     7201 cacatgcgat ttcatttgag ggcagtaagc gcagatgtgt cagtggtgtg tttgtgagtt
     7261 aaaaacacca tggtaaaggc ggtgatttag gtgttggatt ctatttgcac tgttggcgtt
     7321 attgatggct atccatgcgg cctgtaaatg cattttaaga tggcttatgg tatcggctga
     7381 atgaattcat gcagcagatt gctgtgcatt gaacattgag atggagaaaa tgagcgactt
     7441 agactagatg taaagataca aatattgaga aataataagt ggctggggag acttgcgcac
     7501 accccagcca gagagcgttt actgcttgct cacattcagt tggcgcagca gggcagcggt
     7561 ttcaaacaaa ggcaagccca tcacgccgct gaagctgcca cttaagtgtt cgataaacac
     7621 cgctgctgcg ccttgtatgc cataagcgcc tgctttgtca tcggcttcac cgctgtccac
     7681 gtaagcttgg atttcggctt cggtaagcgg cctgaaggtg acatcactga cttgtgtggc
     7741 ttggctgcgt tgaccttgat gccacatcac caccgagctg atgacttgat gcgagcggcc
     7801 tgaaagcatg cgcagcatgg ttactgcatg ggcatggttg tcgggtttgc ctaagataag
     7861 cccatctatg gctaccgagg tgtcggcggt taacacgggt agctcgggtt ttgcgccatg
     7921 agacgccaaa aacaaagcca aggcttgctc ggacttttgc catgccatgc gttcggtata
     7981 ggccaaagcc aattcgtttt cgtggggtgt ctcatcgatg tcgctggcga ttttggtgat
     8041 atgtaggcca atttgtgcca ataattgggc g
//
LOCUS       DAOMVK010000041         7890 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_2390_length_7890_cov_3.281812, whole genome shotgun sequence.
ACCESSION   DAOMVK010000041 DAOMVK010000000
VERSION     DAOMVK010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 7890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7890
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2390_length_7890_cov_3.281812"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..152
                     /locus_tag="PK856_02510"
     CDS             <1..152
                     /locus_tag="PK856_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HRL01276.1"
                     /translation="TSVNPARSTGVALFQGGWAIGQLWLFWIAPIVGAVLGAVIYKAM
                     AGSKN"
     gene            290..1960
                     /locus_tag="PK856_02515"
     CDS             290..1960
                     /locus_tag="PK856_02515"
                     /inference="COORDINATES: protein motif:HMM:NF028537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase"
                     /protein_id="HRL01277.1"
                     /translation="MRLAQSLESVSSRPVDKVTIKRSLPEVGYLLGAAALFTFWINSA
                     YWLRTYQLLNIHGVMDVVFLLSQTLLVWGVTTTVLLLLCWGKLTKPLLTVLFVISAAC
                     AYFSFNYQVYIDRHMLTNVMRTDVHEATGLLSWKLLVWMVIFLSPALAYVWGIKRRPI
                     TKWWRNLGLHLLFAIITLALGLAASLPIYKSYASFFRNNKQVVKMLTPFNFIISSVSY
                     AQHQYRDAHQVFVHVGMDAKQVKPASVTKPTVFVVVVGETGRGDRFSLNGYGRDTNPL
                     LSKQQGLVSFKNAQSCGTATAYSVPCMFSDLSRQDINIDNAPMRDNLLDIVQRTGVKV
                     EWVDNNSGCQETCGRVPTQQLKQDCPEGLCYDEQLVTALQQRLKQPLQADQFLVLHMN
                     GSHGPAYYQRYPKQFEVFKPACNTNEINTCNQEALNNVYDNTVVYTDSVLNGLIDTLK
                     QQQNVNTALIYLSDHGESLGEKGLYLHGTPYAIAPKEQTHIQMLFWANADFYQQHGVQ
                     NTCLQNMANQPEISQDNLFHTVLGGLNVKTQVYNAQLDLFNACSQGKA"
     gene            1970..2644
                     /locus_tag="PK856_02520"
     CDS             1970..2644
                     /locus_tag="PK856_02520"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRL01278.1"
                     /translation="MKVLLVEDDALLQSGIKEALRRDGWFCEATATVREAKKWADATE
                     YALILLDLGLPDGNGLEVLKYVRSQQKNVPIIILTARDEVTDRVMGLDAGADDYLVKP
                     FAISELLARMRSVLRRSEGRSLNLLQSGDLSLDLGQKTVYWAGEDLKLAGREYALLNR
                     LLLRVDQIVARELLLSDVYTWSDQLSSNTLEVYVHHLRQKLPVGAIETVRGQGYRLVS
                     TCLHAA"
     gene            2626..3702
                     /gene="pmrB"
                     /locus_tag="PK856_02525"
     CDS             2626..3702
                     /gene="pmrB"
                     /locus_tag="PK856_02525"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF008025.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HRL01279.1"
                     /translation="MFTRSLKLRLLLVVVTVLVVTQTITALWLWHESQEQVAILVQAD
                     LSDEEMVAQIEHEKFESVLAFVLPALFCVLLSAGLLYFLIRRLTEPLEHLAQGVNQRS
                     AHDLATLPLEKNASAEVVTIVDSTNRLLQRISLGLEHERRFTADVAHELRTPLAGIRM
                     HLELLAFESPDTIGPLIQRIDDLMQLTDQLLQLARASQQSRAGQLQMDDMDLVAEVCL
                     PLQQEFDWYSDLHMEWHLPEHAYCVASAPLLQIALKNLIANARKYGGERPEIKVSVVA
                     EQNEWVLRVCDNGPGVAPELLGQLTDSFFRADQVKQGYGLGLSIVKRITDLHQADLQL
                     TNLAGSGLQVAFRLKRVEALVNVQ"
     gene            complement(3668..4183)
                     /locus_tag="PK856_02530"
     CDS             complement(3668..4183)
                     /locus_tag="PK856_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HRL01280.1"
                     /translation="MVVAARSQIGKTVSYDPAYTVLAYPNGDVDMHKGVCTDVVIRAL
                     RTQGMDLQQLVHEDMRQNFNSYPKLWDLSQADANIDHRRVPNLRRYFERHDYAVQDGR
                     FQAGDIITWDLGKGLVHIGIASDKNNAQGEPLIIHNIGRGTQEEDILRRFKMTGHYRI
                     PTERLQAPQLA"
     gene            complement(4439..5296)
                     /locus_tag="PK856_02535"
     CDS             complement(4439..5296)
                     /locus_tag="PK856_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01281.1"
                     /translation="MGFFSKLFGQKSAERKLSKREFTEQLAQEIQQSIPDILVKVMDA
                     DNDDEISIHVEHPNSQSNIVYPDNLYASYLQEREDFAAYKQNIINNIHDMYFGEEESA
                     TLIPNIKHIDWVKEIEKVSSQSTSDEPAKLVTFPFAGDLVIVIAMDYASKMTYLFESD
                     LPNYTPDGNKDTALQLAQDNLRAHSEHVEIEPIHNTWQQIRFDQTYDASWVLLFEEIA
                     PKLNLVGEPVLALIARDRFIVADSNVPEDVATLKQFAAEQTSQVPHALSAHLYHYQSG
                     HIRLYEALQ"
     gene            complement(5447..5647)
                     /locus_tag="PK856_02540"
     CDS             complement(5447..5647)
                     /locus_tag="PK856_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01282.1"
                     /translation="MSDPTYPKELRFCTNCQQETVHNLHGVYIQTPHFIRLPALNCAR
                     STVCEHYAIDAQGLTVYQHISE"
     gene            complement(5701..7056)
                     /locus_tag="PK856_02545"
     CDS             complement(5701..7056)
                     /locus_tag="PK856_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRL01283.1"
                     /translation="MKLNSIRSFWQQFLLFLLPLIATNILQSLSGTINTVFVGQMMGV
                     NAIAAISVFFPILFCLMAFIIGLSAGATVLVGQAWGKGENEQVQAITGATLFMTLIGG
                     SIIAAVGVYFSRGILVGLGTDASLIPLALPYVQTMLAGSPILFVYIIYTSILRGVGDS
                     KTPLLALGLTSGIGLIVTPTLIQGWFGMPKLGILAPAVATILGYVLVMLFLTVYLNRR
                     RHILSINRTLLSHIRFSPSLSPLILKLGVPTGIQMVTNSLAGLVIIGLVNGFGPQATA
                     AYGAVNQVNNYVQFPAISISIAASIFAAQAIGSGQVQRLAKVTLTALSMNLYLTGSLI
                     VLAYLFADRLMALFITDAAVVALGGELLHIVLWSVLFFGAGSIFAGIMRASGTVLVPM
                     LINMVAVIGIELPLAYWFSHLWGLHGIWMAYAFSFVCLCIMQGQYYHWVWKKKSIEAL
                     V"
     gene            7265..7465
                     /locus_tag="PK856_02550"
     CDS             7265..7465
                     /locus_tag="PK856_02550"
                     /inference="COORDINATES: protein motif:HMM:NF018961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HRL01284.1"
                     /translation="MGIAKIIEISSSSKKSFDDAVKSGIEKASKTIDNIEGAWVNEQK
                     VVVQDGKVVEYRVNLKITFVLK"
     gene            complement(7554..7871)
                     /locus_tag="PK856_02555"
     CDS             complement(7554..7871)
                     /locus_tag="PK856_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HRL01285.1"
                     /translation="MNVLFVCSKNQWRSPTAEKIFAEDYGIRTRSAGTSSSARHRINQ
                     RDLAWADIIMVMEERHKTIIKQQFHPHDYKKIVVLDIADDYLFMDAELIALLQTSVSP
                     YLP"
BASE COUNT         2031 a         1699 c         2099 g         2061 t
ORIGIN      
        1 atacctcagt taaccctgct cgttcaactg gcgtggcctt atttcaaggc ggctgggcca
       61 tagggcaatt gtggctgttc tggattgcgc cgattgttgg tgcggtgttg ggtgcagtga
      121 tttacaaagc catggcaggc agcaaaaact aagctgttta tgcatcacaa gcggcctgta
      181 aacctaaaat gggtttacag gccgctttgt attaaaggtt tcagtggctt aaggtttcat
      241 taagcttggc tctctaaact gagcgcattc tgtttttaga aagactggta tgcgcttggc
      301 tcaatcattg gaatcggtat ccagcagacc tgtagacaaa gtaaccatca aacgcagctt
      361 gcctgaggtg ggctatttgc ttggtgcagc agccttgttt acattttgga ttaacagcgc
      421 ttattggttg cgtacttacc aactgctcaa tatacatggc gtgatggatg tggtgttttt
      481 gctgtcacaa acgttattgg tatggggcgt gaccacaaca gtgttgctgc tgctttgttg
      541 gggaaaactc accaaaccct tgttgacggt gttatttgtc atcagtgcgg cctgtgcgta
      601 tttttcattc aactatcagg tttacataga cagacacatg ctcaccaacg tgatgcgtac
      661 cgatgtgcat gaggccacgg gattgttgtc atggaagctc ttggtgtgga tggtcatttt
      721 cttaagccct gcgctggcgt atgtgtgggg catcaaacgt cggcccatca ccaaatggtg
      781 gcgcaatttg ggcttacact tgttgtttgc catcatcacc ttggccttgg gtttggccgc
      841 cagtttgcct atttacaaaa gttacgccag ttttttccgt aacaacaagc aagtggtgaa
      901 aatgctcacc cctttcaact tcattatctc tagcgtgagc tatgcccaac accaataccg
      961 tgatgcacat caagtgtttg tgcatgtggg catggatgcc aagcaagtga agccagccag
     1021 cgtgaccaag cctacggtgt ttgtggtggt ggtgggtgaa actggccgtg gcgaccgttt
     1081 ctcactcaat ggctatggcc gtgataccaa tcctttgttg agcaagcaac aaggcttggt
     1141 cagctttaag aatgcgcaat cgtgtggcac agccaccgcc tattcggtac cgtgtatgtt
     1201 ttccgatttg agccgccaag acatcaacat cgacaatgct ccgatgcgtg acaatttgct
     1261 cgacatcgtt cagcgtacag gggtaaaagt ggaatgggtg gacaataatt cgggttgcca
     1321 ggaaacatgt ggtcgtgtgc ccacacagca attgaaacaa gattgcccag aaggtttgtg
     1381 ttacgacgag caattggtca ctgccttgca acagcgttta aaacagccat tacaggccga
     1441 tcaattcttg gtattgcaca tgaacggtag ccatggccct gcatattatc agcgctatcc
     1501 aaaacaattt gaggtgttta aaccagcctg taacaccaat gaaatcaata cctgtaatca
     1561 agaagcattg aacaatgtgt atgacaacac tgtggtctat accgattcgg tattgaatgg
     1621 tttgatagac accttaaagc aacagcaaaa tgtgaacacg gcattgattt acctgtctga
     1681 ccacggtgag tctttgggag aaaaaggctt gtatttgcat ggcacacctt atgcgattgc
     1741 gccgaaagag caaacccaca tccaaatgct gttttgggcc aatgccgatt tttaccaaca
     1801 acatggcgtg caaaacactt gtttgcaaaa catggccaat cagcctgaaa tttcgcaaga
     1861 caatttgttt cacacggtat tgggtggttt gaatgtgaaa actcaggttt ataatgcaca
     1921 attggatttg tttaacgcat gttcacaagg caaggcataa ggatagaaaa tgaaagtgtt
     1981 gttggttgaa gacgatgcct tattgcaaag tgggattaaa gaagctttgc gccgtgatgg
     2041 ttggttttgt gaagccacgg ccacggtacg agaggccaag aaatgggcag atgctactga
     2101 gtatgccttg attttgctgg atttgggctt gcctgatggc aatggtttgg aagtattgaa
     2161 atacgtgcgc agccagcaaa aaaatgtgcc catcatcatt ttaaccgcgc gagacgaggt
     2221 gaccgaccgt gtgatgggtt tggatgcggg tgcggatgat tatttggtta agccgtttgc
     2281 catcagtgaa ttgcttgccc gcatgcgttc agtgttgcgc cgcagtgagg gacgcagtct
     2341 gaacttgttg caaagcggtg acttgagctt ggatttgggg caaaaaaccg tgtattgggc
     2401 gggagaggat ttgaaattgg ctgggcgtga atacgcctta ctcaaccgac tgttgctcag
     2461 ggtagaccaa attgtggcgc gcgaattatt gctcagcgac gtttatactt ggagtgatca
     2521 attgagctcg aataccttgg aggtgtatgt gcaccatttg cgccaaaaat taccagttgg
     2581 tgccattgaa accgtgcgtg gtcaaggtta ccgtttggtg tcgacatgtt tacacgcagc
     2641 ttaaaactac gtttgttatt ggtggtggtg acggtgttgg tggtgactca aaccattacc
     2701 gcattgtggc tgtggcatga gagccaagag caagtggcca tattggtaca ggccgatttg
     2761 agcgacgagg aaatggtggc tcagattgag catgagaaat ttgaatcggt gctggcattt
     2821 gtgttgccag cccttttttg tgtgctgttg tcagcgggct tgttgtattt tttgatacgc
     2881 cgcttgactg agccattaga gcatttggcg caaggcgtga accaacgctc ggcccacgat
     2941 ttggctacgc tgccattgga aaaaaatgcc agtgcagaag tggtaaccat cgtggacagc
     3001 accaatcgtt tgttgcaacg catctccttg ggtttggaac atgagcgcag gtttactgcg
     3061 gatgtggcgc acgaattgcg cacgcctttg gcaggcatac gcatgcattt ggaattattg
     3121 gcctttgagt caccagacac cattgggccg ctgatacagc ggattgatga tttgatgcag
     3181 ttaaccgacc aattgttgca attggccaga gccagtcaac aaagccgcgc agggcaattg
     3241 caaatggatg atatggattt ggtggcagag gtgtgtttgc cattgcagca agaatttgat
     3301 tggtacagtg atttgcacat ggaatggcat ttgcctgaac atgcttattg tgtggcttcg
     3361 gcaccattgt tacaaattgc cttgaaaaat ctgatagcaa acgcacgcaa atatgggggt
     3421 gagcggcctg aaattaaggt gagtgtggtg gctgaacaaa acgaatgggt gttgagggtg
     3481 tgtgacaatg ggcctggtgt tgctcccgaa ttattggggc agctaaccga cagctttttt
     3541 cgagccgatc aagtcaagca aggctatggt ttgggtttga gcatagtgaa gcgcattact
     3601 gatttgcacc aagccgattt gcagctgacc aatctggcag gttcgggttt gcaagtggcg
     3661 ttcaggctta agcgagttga ggcgcttgta aacgttcagt aggaatgcgg tagtggccgg
     3721 tcattttgaa gcggcgcaat atgtcttctt cttgggtgcc acggccgatg ttatgaatga
     3781 tgagtggttc accttgcgcg ttgtttttgt ctgaagcgat gcctatatgt accaaacctt
     3841 tgcccaaatc ccaagtgatg atgtcgcctg cttgaaagcg gccatcctgc acggcatagt
     3901 catggcgctc aaaatagcgg cgcaaattgg gcacgcggcg gtggtcgatg ttggcatcag
     3961 cttgggataa atcccagagt ttggggtagc tgttaaaatt ctgtcgcatg tcttcatgca
     4021 ccaattgctg taaatccatg ccttgggtgc gcagcgcccg tatgaccaca tcggtacaca
     4081 cgcctttgtg catgtccaca tccccattgg ggtaagccaa aacagtgtaa gcagggtcgt
     4141 aggagacggt cttgccaatt tggctgcgag ccgccaccac caatggtggt gagggctgag
     4201 tcgcttgggc ttgcgtggct gaggcgctgg ggtttaatgg gttaggaagg atgatgacat
     4261 cgtgcccatt ttcgggcgta ggcgagacac ttggtgtggt gttgattggc ggcgctgcat
     4321 ctggccatgc tgcccaaatg accaagccta atagtgcggc tgcaaaaggg tagcgcagtt
     4381 tcatggtaat acctcctctg gggttaaaac gatatggtca ccttaatggt gaccatgctc
     4441 attgtaaagc ttcatacaaa cggatgtgcc cactttgata atggtataaa tgagcggaca
     4501 gcgcatgggg aacttgcgag gtttgttccg ctgcaaattg ctttaaggtc gcaacgtctt
     4561 cagggacatt gctgtctgcc acgatgaaac ggtcgcgagc aatgagtgcc aatacaggct
     4621 cgcccaccaa attcaacttt ggcgcaattt cctcaaacaa caacacccag cttgcatcgt
     4681 aagtttggtc gaaacggatt tgttgccaag tgttgtgtat gggttctatt tcaacgtgtt
     4741 ctgagtgagc ccgtagattg tcctgagcca gctgcaaggc cgtgtcttta ttgccatctg
     4801 gggtgtaatt tggcaaatcg gattcaaaca aataagtcat tttgctggca taatccatcg
     4861 caatcacaat gaccaagtct ccagcaaaag gaaaagtcac cagtttggcg ggctcatcgc
     4921 tcgtgctttg agaggagact ttttcaattt ccttgaccca atcaatgtgt ttgatgttgg
     4981 gtatcagcgt ggcgctttct tcctcaccaa aatacatgtc gtgaatgttg ttgatgatgt
     5041 tttgtttgta agcggcaaaa tcttcccgtt cttgtagata actggcatac agattgtcgg
     5101 gatagacaat gttggattga gagttggggt gctctacgtg gatgctgatt tcatcgtcat
     5161 tgtctgcatc catgactttg accaagatgt cgggtatgga ttgctggatt tcttgagcca
     5221 attgctcagt gaattcgcgt ttggaaagct tgcgttcggc actcttttgg ccgaacagtt
     5281 tggaaaaaaa tcccatgatg gtctcctgtg gcagggcaaa tattgttgct tgcgtaagca
     5341 acactgtgca ggtggaacat tgtgctttaa ttcttcaatg atactgtgtt ttgaatcggt
     5401 ttacaggccg cagtgtgagc ataagcggcc tgtaaaagac agctatttat tcagaaatat
     5461 gttggtacac ggttaggcct tgggcatcaa tcgcataatg ctcacacacc gtactgcgtg
     5521 cacagttcaa tgcgggcagg cggataaagt gtggggtttg gatgtaaacc ccgtgtaagt
     5581 tgtgtacagt ttcttgttgg cagttggtac agaagcgcag ttctttgggg taggtagggt
     5641 cagacatgtg ggctccaaaa tgaaatgaga gaaacacatt caagcaagat gggcggcatc
     5701 ttacaccaaa gcctcaatgg attttttctt ccatacccaa tggtagtatt gcccttgcat
     5761 gatgcacaaa cacacaaacg aaaaagcata agccatccaa atgccgtgta aaccccatag
     5821 gtggctaaac cagtaggcca gcggcaactc tatgccgatt actgccacca tattaatcag
     5881 catcggcacc agcaccgtgc cactggcccg catgatgccc gcaaaaatac ttcccgcacc
     5941 aaaaaacaac acactccaca aaacgatgtg taacagctcg ccgcccaacg caaccacggc
     6001 agcatcggta atgaacaatg ccattaaacg gtcggcaaac aaatacgcca acacaatcaa
     6061 actgccggtt aaatacaaat tcatgctcaa ggccgttaaa gtgactttgg ctaagcgctg
     6121 tacttgaccc gaacctatgg cttgagccgc gaagatggag gctgcgatgg aaatggaaat
     6181 ggcaggaaac tgcacataat tgttcacttg gttgacggcg ccataagcgg ctgtcgcttg
     6241 tgggccaaac ccattgacca aaccgataat aaccaagcct gcaagcgagt tagtcaccat
     6301 ttgtatgcct gtgggtacgc ccaattttaa gattaacggt gacagcgagg gcgagaaacg
     6361 tatgtggctg agcaaggtgc ggttgatgct caaaatgtgg cgacggcgat tgaggtaaac
     6421 ggttaggaac agcatcacca gcacatagcc caaaatcgtc gccacagcag gcgccaaaat
     6481 gcccaacttt ggcatgccaa accagccttg aatcaaggtg ggggtcacaa tcaaaccgat
     6541 gccactggtc aggcccagag ccaagagtgg ggtcttgctg tcgccgacgc cgcgcagtat
     6601 tgaggtgtag atgatgtaga caaacaagat ggggctgccc gccaacatgg tttgtacata
     6661 cggcagtgcc aatggaatca ggctcgcgtc tgtgcccaag cctactaaaa taccgcgtga
     6721 aaaatacacg ccaacggcgg cgatgatgct gccgccgatt aaagtcatga acaaggtggc
     6781 acccgtaatg gcttgcactt gctcattttc ccctttgccc caagcctgac ccaccaacac
     6841 ggtggcccca gcagacaagc cgatgatgaa ggccatcagg caaaacaaaa tcgggaaaaa
     6901 caccgaaatc gccgcgatgg cgttcacccc catcatctgc cccacaaaaa cagtgttgat
     6961 ggtgccagag agcgattgca aaatattggt tgcaatcaaa ggcagaagaa acagcaaaaa
     7021 ttgttgccaa aaagaacgga ttgaattcaa tttcatcggg ttgttttagg gatgaagttc
     7081 gaatgtttct ttgtatcatg taaaacaacg cgggtggtga ggtttggctg gattaatggg
     7141 gtggtgagtg taaatatttg ttttaagtta gcctaagaga ataacgggct gaactttgtg
     7201 gcaggcatgt ccagtctaat cgctgcgttt gaacgcagaa aaacaactac caaaggagaa
     7261 gagtatgggc atagccaaaa tcattgaaat cagctccagc agcaaaaaaa gctttgacga
     7321 tgctgtgaaa agcggcatcg aaaaagccag taaaaccata gacaacatcg aaggtgcttg
     7381 ggttaacgaa caaaaagtgg tggtgcaaga cggtaaggta gtggaatacc gtgtgaattt
     7441 gaaaatcacc tttgtattga aataagtact gctttaaaac tgttttgtat gcggcctgta
     7501 aacctaggtt ttctttcgaa acttaaggtt tacaggccgc attgacatgg gacttagggc
     7561 aagtaggggc tcacactggt ttgtaacaat gcgatgagtt ctgcatccat aaataagtag
     7621 tcatcggcga tgtccaacac gacgattttc ttgtaatcat gtgggtgaaa ctgttgtttg
     7681 atgatggtct tgtggcgctc ttccatcacc atgatgatgt cggcccaagc caaatctcgt
     7741 tggttaatgc ggtggcgggc actgctgctg gtgcctgcgg agcgtgtccg tatgccataa
     7801 tcttcagcaa aaatcttctc ggcagtaggg ctgcgccatt ggtttttact gcacacaaat
     7861 aaaacattca taagcggcct gtaaactggt
//
LOCUS       DAOMVK010000042         7822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_2424_length_7822_cov_2.348268, whole genome shotgun sequence.
ACCESSION   DAOMVK010000042 DAOMVK010000000
VERSION     DAOMVK010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 7822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7822
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2424_length_7822_cov_2.348268"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PK856_02560"
     CDS             <1..268
                     /locus_tag="PK856_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003769012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRL01286.1"
                     /translation="FVAFGITFETPVIVVLLCRMGVVSVAQLRAARAYVIVGAFVVAA
                     IVTPPDVISQIMLAVPIIVLYEAGVQVCRFVRPKAAELVEVVDE"
     gene            312..638
                     /locus_tag="PK856_02565"
     CDS             312..638
                     /locus_tag="PK856_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003791322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HRL01287.1"
                     /translation="MLCVISLVLLIAVCVAWESFLAPLRSGGSWMVLKVLPLLAILPS
                     ILKGRRYTYQYSSMLILFYFTEGVMRLFDADALSRVCAGLEVLFSVIFFVACLIFCRN
                     TRVSHE"
     gene            631..>1527
                     /locus_tag="PK856_02570"
     CDS             631..>1527
                     /locus_tag="PK856_02570"
                     /inference="COORDINATES: protein motif:HMM:NF027197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin glycosylation ligase domain-containing
                     protein"
                     /protein_id="HRL01288.1"
                     /translation="MNSLVSFYRKSFGQALAIASLMCLMSVPFLMNVRVGPQGGFIIE
                     STSAILALCMVLGVTLMGKFTVKPPLVSVMFAVFALIISLQARLMDISWVSQVDLTSS
                     VLLLAALSAWALASWQAQSETPLMRWIALGLLIGVLLQTAVMWIQYFGITTHFKGIIS
                     YSTTTNIMGQLGQRNHLGHYLMWGMVAVLYLNHTKTLSRWLSMAAVFWLGLSMGLIGS
                     RTLIAYLMAISLLAIIWRFWAGRAYQSNLYWILLTLLWVLLVQFGLKKVLDAFNQSKT
                     TTGLERLVNAADVVDTARSVEWQ"
     assembly_gap    1528..1537
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1538..2424
                     /locus_tag="PK856_02575"
     CDS             <1538..2424
                     /locus_tag="PK856_02575"
                     /inference="COORDINATES: protein motif:HMM:NF023274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
                     /protein_id="HRL01289.1"
                     /translation="PWVGHGWGSSSSQSVILHGALPDFAGLRTVGVLFTHSHNSLLQL
                     LSEVGLLGTLWLAGCFAVMVVVCFKYAKQPESFFILALASITVCHSLLEYPLWYVYFL
                     LPFALFWALVPRSAEARLVPQDGCKKLNLAAALTTVMALAVCIRLLFAYNDIMSVYGV
                     AKNESPEVAASKKAKIHALIEREYLLDYYARMALIERSFDAYQGAEVADEVENARLVA
                     EYRPYNSYAIRWGMYQYRTGQEAAARKWMEEVWLYYPASIAGNVTAMQKSLWYTDLIP
                     LAKQRCLQYQQKVKITCP"
     gene            complement(2506..3693)
                     /locus_tag="PK856_02580"
     CDS             complement(2506..3693)
                     /locus_tag="PK856_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HRL01290.1"
                     /translation="MLDNSLSRRQLMRFGGAGLGAAVLTACGTNKTPVVVEPIKSRPK
                     TQTGRGGNQLLRIFACSGFLDKSERVNTAVNRLNACGFQVSNAEAAYRRYQRFAGSDF
                     QRVQDLQDVATGRVATPKVLLGARGGYGAIRLLPQINWDGLGGRMREQGTLLMGYSDV
                     CAIQLALLAQTGVPSFAGPMLYSEFGSPNPSTFTMQNFIDGTTQAEHTVNIGQIQSRE
                     INVSGTFWGGNLSVLQSLAGTPYMPDINGGILFIEDVNEQPYRIERALQTLHLAGILK
                     KQQAIVVGDFRMGNIRDAYDSSYTLSSVMQTIQSAVKVPVLTGFPFGHISNKVTMPLG
                     ARAQIRSSSNGGYQVRFSDYPTLNPQSLNLSQLLPQQSFDGLDFIGGHTGSAQDAQII
                     EAE"
     gene            complement(3680..4111)
                     /locus_tag="PK856_02585"
     CDS             complement(3680..4111)
                     /locus_tag="PK856_02585"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HRL01291.1"
                     /translation="MSACPLCEPQAETVLFQNEQLRVILVTDQVLVPAYCRVIWLKHV
                     AEMTDLSANERQALMDMVYFVESQMRHILQPQKINLASFGTMVPHQHWHVIARFSDDA
                     YYPDSIWSVAHHQNTPALPDDWITRLQAALNAYEGASHAGQ"
     gene            complement(4223..6508)
                     /locus_tag="PK856_02590"
     CDS             complement(4223..6508)
                     /locus_tag="PK856_02590"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HRL01292.1"
                     /translation="MDELLKEAALNFHEYPNPGKIQITPTKPLATQYDLSLAYSPGVA
                     APCMEIYNDPLASYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEINETDPDKLVDIIASLEPTFGGINLEDIKAPECFYIEKKLRERANIPVFH
                     DDQHGTAIITAAAVLNGLRVIEKDIKEVHVVCSGAGAAAIACLDLLVALGMTRENITV
                     CDSKGVIYKNRDDKMDASKQRYAIEDNGQRVLADAVNGKDIFLGLSGPNVLSVEMLKT
                     MKQDPIVFALANPQPEIWPPEAIAARPDVIIGTGRSDFPNQVNNVLCFPFIFRGALDV
                     GATTINEEMKVACVLAIADLALAEQNDQVASAYGNASLSFGREYLIPKPFDPRLIVKI
                     APAVAKAAMDSGVATRPIEDFDAYVEKLDQFVYKSNLFMKPVFTKAKTQLKRVVLCEG
                     EDLRVLHATQQIVSQKLAFPVLIGRPEVIERRIKELGLNLQAGKDFELVNNQRDPRYR
                     EYWGEYYQSMKRKGITEEMARRRVIGNTTLIGALMVNRGDADAMICGTFGRYREHFDT
                     IEQVIGYNNPEKVAGAMNALILPHGNIFITDTYVNAKPNAAQLAKITEMATEDMRRFG
                     IEPKVALMSNSNFGSIDNDTSRLMQDTLELVRAAQPDLEVDGEMQGDVALMEEMRHSI
                     LPDSTLNGSANLLVMPNVEAANISYNLLRVSAADGVTIGPILMGLNKPVHILTPISSV
                     RRIINMVAVAAVEAQRQDEEA"
     gene            complement(6538..7086)
                     /locus_tag="PK856_02595"
     CDS             complement(6538..7086)
                     /locus_tag="PK856_02595"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HRL01293.1"
                     /translation="MAVLKTQTVSKPWYKEAWAWGILSGPLLVIPAGLITYYIAASGA
                     DPLVSDDYYKDGKNIHLQMDRDSAASSHHIQAKVLFSPDGSKVRVFLQGDYDPKNTLH
                     FSLQHPTMAQYDQAVALTLINADAKLYEAQLKPLPPMNSWYVRIENDQATWRVQEKWL
                     VQNGNSLALTPKYATVAASSPK"
     gene            complement(7089..>7822)
                     /locus_tag="PK856_02600"
     CDS             complement(7089..>7822)
                     /locus_tag="PK856_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003787256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FixG Ig-like domain-containing protein"
                     /protein_id="HRL01294.1"
                     /translation="MFDKDTLLVSYDNERGEPRGARKKNQEATPLGDCVNCTLCVQVC
                     PTGIDIRDGLQYQCIGCGACIDACDEVMDKIGSPRGLIRYTTEAALAHEYADKDIKKR
                     LLRPRVLGYGLILFLVSCGLLYGIFSRDLMNIDIIKDRGVMVRQNNEGLLENAYNLRL
                     SNASEKTLYVQTKVSGFDHIELTGLPETLEIKPGDIVNIPVQVATYPEYATKGSHPIE
                     FSFSYHTEDKQEWRTINEDSNFIGE"
BASE COUNT         1916 a         1766 c         1921 g         2209 t
ORIGIN      
        1 gtttgtggct tttggcatca cgtttgaaac ccctgtgatt gtggtattgc tgtgccgcat
       61 gggtgtggtc agtgtggcac agctgcgcgc agcccgtgca tacgtcattg ttggggcatt
      121 tgtcgtggcg gcaatcgtga ccccgcccga tgtgatttca caaattatgt tggcagtgcc
      181 catcattgtg ttgtacgaag cgggggtgca agtgtgccga tttgtcagac ccaaagcggc
      241 agagttggta gaggttgtcg atgagtaatg aggtggtttt ggctttgaat gcgcgtatac
      301 agcaagcgcg catgctgtgt gtcatcagtt tggtgttgtt gattgcggtg tgtgtggcat
      361 gggagtcgtt tttggcaccg ctgcgcagtg gcggctcatg gatggtgtta aaagtattgc
      421 ctttactggc catattgccc agtattttaa aaggccgtcg ctatacctac caatacagca
      481 gcatgttgat attgttttat tttaccgaag gcgtgatgcg cctttttgat gccgatgctt
      541 tgagccgcgt gtgtgcgggt ttggaagtgt tgttcagtgt gattttcttt gttgcgtgtc
      601 tgattttctg ccgcaatacg cgtgtgtccc atgaatagtc ttgtcagttt ctaccgtaaa
      661 tcgtttggcc aagctttggc cattgcctct ttgatgtgtc tgatgtccgt ccctttctta
      721 atgaatgtgc gtgttgggcc acagggtggg ttcattattg agtcgacttc agccatcttg
      781 gccttgtgca tggtgcttgg ggtgacattg atgggtaaat ttactgtcaa accgcctctt
      841 gtcagcgtga tgtttgcggt atttgccctc atcatttctt tacaagcccg cttgatggac
      901 atcagttggg tgtctcaagt ggacttaact tccagcgtgt tgttattggc ggcattgagc
      961 gcttgggcac tggccagttg gcaggcacaa agtgaaactc ctttgatgcg atggattgcc
     1021 ttaggtcttc tgataggtgt gttgctacaa acggcagtga tgtggattca gtattttggt
     1081 ataacgactc actttaaagg cattatttca tattcgacga ctacaaatat catggggcaa
     1141 ttggggcaac gcaaccattt gggccattat ttgatgtggg gaatggttgc agttttgtac
     1201 ttgaaccata ccaagacatt atcccgttgg ctaagtatgg cagcagtgtt ttggttggga
     1261 ttgagcatgg gcttgattgg ctcacggact ttgattgcgt atttaatggc aatcagcctc
     1321 ttggccataa tctggcgttt ttgggcaggg cgtgcctatc aatccaattt gtattggatt
     1381 ttgctaactt tgctgtgggt tttactggtg cagtttgggt tgaaaaaagt attggatgct
     1441 tttaatcagt ctaaaacaac aacgggtttg gaacgacttg ttaatgcggc cgatgtggtt
     1501 gatactgcgc gcagtgttga gtggcaannn nnnnnnngcc catgggtagg gcatgggtgg
     1561 ggaagttcat cctcccaaag tgtcatatta catggggctt tacctgattt tgcgggtttg
     1621 cgcacagtag gggtgttgtt cacccatagt cacaattctt tactgcaatt gctttccgag
     1681 gtgggtttat tggggacatt gtggttagca ggctgttttg ccgtcatggt agtggtgtgc
     1741 tttaagtatg ccaagcagcc tgaaagcttt tttatcttgg ctttggcgag catcaccgtg
     1801 tgccacagtt tgttggaata cccattgtgg tatgtgtatt tcttgttgcc attcgccttg
     1861 ttttgggctt tggttccgcg ttcagctgaa gctaggctgg tgccacaaga tggctgtaaa
     1921 aaattaaatt tggcagcagc gcttaccaca gtaatggctt tggcagtgtg catccgttta
     1981 ttgtttgcat ataacgacat catgtcggta tatggtgtgg ccaaaaatga aagccctgaa
     2041 gtggctgcaa gcaaaaaagc caagatacat gctttaattg aacgtgagta tttgttggac
     2101 tactatgccc gcatggcctt gatagagcgc agttttgatg cttaccaagg agcagaggtc
     2161 gctgatgagg tagaaaatgc gcgtttggtg gccgaatacc ggccttataa ttcttatgcc
     2221 atacgctggg gcatgtacca ataccgtaca gggcaagaag cagcggccag aaaatggatg
     2281 gaggaggtgt ggttgtatta ccctgcttcc atcgctggta acgtgacggc gatgcaaaaa
     2341 tcgttgtggt ataccgattt aattccactg gcgaagcaac gctgtttgca ataccagcaa
     2401 aaagtgaaaa ttacctgtcc ttaatattaa gtcagtacca attaaagcgg cctgtaaact
     2461 tacaggccgc tttattgatt catgaggtaa cgtttcttaa tactgctatt cagcttcgat
     2521 gatttgcgcg tcttgcgctg aacctgtgtg gccaccaatg aaatccaagc catcaaacga
     2581 ttgctgtggc aacagttgtg acaggtttag actttgtgga tttaaagtag ggtagtcact
     2641 gaagcgcact tggtaaccac cgttggaaga agagcgtatt tgtgctcgcg ctcccaaagg
     2701 catggtgact ttattgctga tgtggccaaa tggaaagcct gttaacactg gcactttcac
     2761 ggcactttgt atggtttgca tcacgctgga cagcgtatag cttgaatcgt aagcatctcg
     2821 aatattaccc atgcggaaat caccaaccac aatggcttgc tgttttttca aaatgcctgc
     2881 caggtgcagg gtttgtaaag cccgttcaat gcgataaggc tgttcattca cgtcttcaat
     2941 gaacaagatg ccaccattga tatcaggcat ataaggggtg cctgccagtg attgcaacac
     3001 gctcaagttt ccgccccaga aggtgccgct gacattgatt tcacgcgatt gtatttggcc
     3061 aatattcaca gtgtgttcgg cttgggtggt gccatcaatg aagttttgca tggtgaatgt
     3121 gcttgggttt ggactgccaa actcgctgta cagcatgggg cctgcaaagc ttggtacgcc
     3181 tgtttgcgct aacaaagcca attggatggc acacacgtca ctgtaaccca tcaacaaagt
     3241 gccttgttcc cgcatgcggc caccgaggcc atcccaattg atttggggca ataaacgtat
     3301 cgcaccatag ccgccacggg cacccagcaa cacttttggg gtggccacac gacctgtggc
     3361 cacgtcttgt aagtcttgca cacgttggaa atcagaacct gcgaagcgtt ggtagcgtcg
     3421 gtaagctgct tcagcattcg atacttggaa accacaggca ttcaaacgat tgactgcggt
     3481 attcacgcgt tccgatttgt ctaggaagcc tgagcaggcg aaaatacgta acaattggtt
     3541 gcctccacgg ccagtttggg ttttgggacg tgatttgatt ggctcaacca cgacaggggt
     3601 cttgttggtg ccacatgcgg ttaataccgc agcacccaat ccagcgccac caaaacgcat
     3661 caattgacgg cgacttaagc tattgtccag catggcttgc tccttcatag gcattcagtg
     3721 ctgcttgcag gcgtgttatc caatcatcgg gcagtgcagg agtgttttga tggtgggcca
     3781 cagaccagat ggaatcagga taatacgcat catcgctgaa tcgggcaatc acatgccagt
     3841 gttggtgtgg caccatggtg ccaaagctgg ccaaattgat tttttgaggc tgtaaaatgt
     3901 ggcgcatttg gctttcgaca aaatacacca tgtccattaa ggcctggcgc tcattggccg
     3961 ataagtcggt catttctgct acatgtttca gccaaatcac acggcaataa gctggaacca
     4021 acacttggtc agtcaccaaa atcacccgca gttgttcatt ctgaaacaac acagtttcgg
     4081 cttggggttc acacaatggg caagcagaca tggtaagctc aaaagtaaaa gaaagcctat
     4141 tatagcaatg gctccaatgc tattgggttt agcaatgaaa aagccgctct aaaagagcgg
     4201 cctgtaaatg cttcaaaatc aattaagctt cttcgtcttg gcgttgggct tcaaccgcag
     4261 ccacagccac catattgatg atgcggcgta ccgaagaaat aggcgttaag atatgaactg
     4321 gtttgttcaa acccatcaaa atcgggccta tggtcacacc atctgcagca gaaacacgca
     4381 ataagttgta gctaatgttt gcagcttcaa cattaggcat taccaacaag ttggcagagc
     4441 cgtttaaggt actgtctggc aaaatagagt ggcgcatttc ttccatcaaa gccacatcgc
     4501 cttgcatttc gccgtctact tccaaatcag gctgtgccgc gcgcaccaat tctaaggtgt
     4561 cttgcatcaa acgtgaggtg tcgttgtcga tagaaccaaa gtttgagtta gacatcaagg
     4621 ccactttagg ctcaatacca aaacggcgca tgtcttcggt agccatttca gtgatttttg
     4681 ccaattgagc ggcattcggc ttggcgttga cataggtgtc ggtgatgaag atgttgccat
     4741 gtggcaaaat caaggcattc attgcaccgg ccactttttc aggattgtta tagccaatca
     4801 cctgctctat ggtatcaaaa tgctcacggt agcggccaaa cgtgccacaa atcatggcat
     4861 cagcatcacc acggttgacc atcaaagcac caatcagtgt ggtattgcca atgacacggc
     4921 gacgcgccat ttcttcggta atgcctttgc gtttcatcga ttggtaatac tcgccccagt
     4981 attcacggta acgtggatcg cgttggttat tcaccaattc aaaatcttta cccgcttgca
     5041 agttcaaacc caactctttg atgcggcgct caatcacttc agggcggcca atcaacacag
     5101 ggaatgccaa tttttgtgac acaatttgct gggtcgcatg caacacacgc aaatcttcac
     5161 cttcacacaa caccacacgt ttcaattggg ttttggcttt ggtgaacaca ggcttcatga
     5221 ataaattgga tttataaaca aattgatcca atttctccac ataagcatca aagtcttcga
     5281 ttgggcgggt tgccacacct gaatccatag ctgctttggc cacagcaggg gcaattttta
     5341 caatcaaacg aggatcaaaa ggttttggaa tcaggtattc acgaccaaac gacaaagaag
     5401 cattgccata ggcagaagcc acttggtcat tttgttcggc caaggccaag tcggcaatgg
     5461 ccaacacaca ggccactttc atttcttcat tgattgtagt ggcacccaca tccaatgcac
     5521 cgcggaaaat gaaggggaag cacaatacat tgttcacttg atttgggaag tcagagcggc
     5581 ctgtaccaat gatgacatca gggcgagctg caatggcttc aggtggccaa atctctggtt
     5641 gtggattggc caatgcgaaa acaatagggt cttgtttcat ggttttgagc atttctacgc
     5701 tcaagacatt cgggccagac aggcctaaga aaatgtcttt accattcacc gcgtcggcta
     5761 atacgcgttg accattgtcc tcaatggcat aacgctgctt ggacgcatcc attttgtcgt
     5821 cgcggttttt gtaaattaca cctttggaat cgcacaccgt gatgttttca cgggtcatgc
     5881 ccaaggccac caacaagtcc aaacatgcaa tggccgctgc acctgcacca gagcacacca
     5941 catgaacttc tttaatatct ttttcaatca cgcgcaggcc gtttaaaaca gctgctgcag
     6001 taatgatggc agtaccatgt tggtcatcat ggaaaacagg aatattggcg cgctcacgca
     6061 gtttcttttc aatgtagaaa cactcgggcg ctttgatgtc ttctaagtta atgccgccaa
     6121 acgtaggctc aagtgaggca ataatgtcga ccagtttgtc agggtcagtt tcatttattt
     6181 caatgtcaaa cacatcaatg cccgcgaact ttttgaacag tacgcctttg ccttccatca
     6241 caggtttgcc tgccaaagca ccaatattac ccaagcccaa aaccgcagta ccgttagaga
     6301 tgaccgctac caaattgcca cgcgcagtgt atttgtaaga ggccaatgga tcattataaa
     6361 tttccataca cggagccgcc acgccaggcg aatatgctaa tgacaaatcg tattgggttg
     6421 ccaaaggctt ggtaggggtg atttgaatct tacccggatt agggtattca tgaaaattga
     6481 gcgcggcttc tttgagcaac tcatccataa agcatttctc caaaattctg ttatgtgcta
     6541 cttaggtgac gaggcggcga cggtagcgta cttgggtgtc agtgccaggc tattaccatt
     6601 ttgcaccagc catttttctt gtacacgcca agtcgcttga tcgttttcga ttctaacata
     6661 ccaactattc attgggggca atggtttcag ctgggcttca tacagcttgg catcagcatt
     6721 tatcagtgtc aatgccaccg cctgatcata ctgcgccatg gttggatgtt gcaaggagaa
     6781 atgcagggtg ttcttagggt cataatcacc ttgtaaaaac acccgaacct tactgccatc
     6841 agggctgaaa agcacttttg cttggatgtg gtgactggat gcggcgctgt ctctgtccat
     6901 ttgtaaatga atgtttttgc catctttgta atagtcatcc gacaccaatg gatcggcacc
     6961 actggccgca atgtaatatg tgattaatcc agcaggaatg accagtaatg ggcctgataa
     7021 tatgccccaa gcccaagcct ctttatacca aggtttgctt accgtttgtg ttttcaaaac
     7081 cgccatgatc attccccgat aaaattggag tcttcgttaa tggtgcgcca ttcttgtttg
     7141 tcttcagtat ggtaactgaa ggaaaactct atgggatggc tgcctttggt tgcatattct
     7201 ggataggttg ccacttggac agggatgttg acaatgtctc ctggcttgat ttccaaggtc
     7261 tcaggtaagc cagtcaattc gatgtgatca aatccactga ctttggtctg tacatacagg
     7321 gttttttcag aggcattact caaacgcagg ttgtaagcat tttccaataa accttcgttg
     7381 ttttgcctta ccatcacccc tcggtctttg atgatatcaa tgttcatcaa atcccttgaa
     7441 aaaatgccat acagcagtcc acaactgacc aaaaataaaa tcagtccata gcccaaaaca
     7501 cgtgggcgca ataaacgctt tttgatgtct ttatcggcat attcgtgcgc taaagcagct
     7561 tcagtggtgt aacgtatcaa gccacgtggg ctgccaatct tatccatgac ctcatcacag
     7621 gcatcaatac acgcaccaca gcctatgcat tggtattgca aaccatcacg tatgtctatg
     7681 cctgttgggc acacttgcac gcacaaagta cagttgacgc agtcgcccag tggggttgcc
     7741 tcttggtttt ttttgcgtgc accgcgtggc tcaccgcgct cattgtcata tgaaaccaat
     7801 aaggtgtctt tatcgaacat gg
//
LOCUS       DAOMVK010000043         7791 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_2447_length_7791_cov_3.066313, whole genome shotgun sequence.
ACCESSION   DAOMVK010000043 DAOMVK010000000
VERSION     DAOMVK010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 7791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7791
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2447_length_7791_cov_3.066313"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PK856_02605"
     CDS             complement(<1..514)
                     /locus_tag="PK856_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HRL01295.1"
                     /translation="MAVHLKPKNAQELLTIDGVQLFVGQAGIKKPDYNDVTLMVLGPN
                     SRVAGVFTQNRYCAAPVRVCQELLPSTNIRALVVNTGNANAGTGEDGLKRARAVCAAV
                     AEQIKCEANQVLPFSTGVILEPLPHEKIQAAIKKMKPVHWDIAAKAIMTTDTVAKSGS
                     RELVVDGEHVR"
     gene            675..1310
                     /locus_tag="PK856_02610"
     CDS             675..1310
                     /locus_tag="PK856_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003782675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HRL01296.1"
                     /translation="MSIIAIDVDPQRTFSPLCPNELPVLEGDQIVDELNLQAALASKR
                     VLTKDAHNPHAAWVVASHEQIGTPIGLPNSDIGWVRHAEVGTDGFKALPGLPEPEQYD
                     FLVYKGVENNMHPYGACYHDLGNTISTGLLEWLTVNGAEHVLVGGLATDYCVKNTVLQ
                     LCAYNPNWTVWLNLAACRGIAAETVISALDEMRTAGAHIVNNSAALRQQLA"
     gene            1374..1724
                     /locus_tag="PK856_02615"
     CDS             1374..1724
                     /locus_tag="PK856_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HRL01297.1"
                     /translation="MIYQGSCHCGATRYELEADPITEVIECNCSHCQRKGLLLSFHPQ
                     TQLRIHSGEAQLTQYFFNKHVVQHMFCPVCGCEPFAIGQDPEGYAVAAINVRCLENID
                     LDELKRQPFDGKSV"
     gene            1783..2364
                     /locus_tag="PK856_02620"
     CDS             1783..2364
                     /locus_tag="PK856_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009115889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRL01298.1"
                     /translation="MSKKILMLVGDYAEDYETMVPFQFLLGVGYQVDAVCPDKKAGDS
                     IKTAIHDFEGAQTYSEKPGHNFALNADFAKVNTADYVGLVIPGGRAPEYLRLNPRVLD
                     IVREFHQAQKPIAAVCHGAQILAAAEIIEGKTVSAYPACAPEVRLAGGVYAEIAIDAA
                     VTDGHLVTAPAWPAHPQWLAQLIAALGAKIDLG"
     gene            complement(2444..2791)
                     /locus_tag="PK856_02625"
     CDS             complement(2444..2791)
                     /locus_tag="PK856_02625"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HRL01299.1"
                     /translation="MSDELNQMQCEACRAGAPQVSDEELPELLRQIPDWQPIVVDGVL
                     QLQRVYTFRNFKQAWAASNKVAELAEQEGHHPALLLEWGKLTVTWWTHAISGLHKNDF
                     ICAAKTDAVLSAE"
     gene            complement(2853..3320)
                     /locus_tag="PK856_02630"
     CDS             complement(2853..3320)
                     /locus_tag="PK856_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01300.1"
                     /translation="MTQLDVEMEAVLLQAIDVHIVLAHELMHKLITETDQPEKALIAN
                     GYFDEIQNADVYAGGETLSGAWWFDVHGEHCLFHNLETGQKLEVALGNPDCVATVDAY
                     FFDQFLASSKKFQSLSLCTEQQFGGVLPVFEQLTRENKMQHVRGVVFSKAWLA"
     gene            complement(3378..4166)
                     /gene="phhA"
                     /locus_tag="PK856_02635"
     CDS             complement(3378..4166)
                     /gene="phhA"
                     /locus_tag="PK856_02635"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004505 - phenylalanine 4-monooxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HRL01301.1"
                     /translation="MDEPFVYTARPSDEHGHIDWSETEDHTWHQLITRQLGNLQDKAC
                     DEYLQGLERLDLPLDHIPALKDINRVLYDATGWQCAEVPALISFDRFFKLLSERKFPV
                     ATFIRRPQDMDYLQEPDIFHEIFGHCAMLTHPAFADFTAHYGKLGVAASKQERAYLAR
                     LYWFTVEFGLLNTAQGLKIYGGGILSSPQETEYAHASAVPERREFNISDVLRTRYRID
                     VLQPIYYQLNSVNDLYDLANTDIMAEVAKARELGLFPLPESIAH"
     gene            complement(4526..5158)
                     /locus_tag="PK856_02640"
     CDS             complement(4526..5158)
                     /locus_tag="PK856_02640"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRL01302.1"
                     /translation="MDDLNNIYHSTQVEVAAAIDDSNELTLATAVQRIVAWLINRVIE
                     VVLMIPAFGLIIVLLFKSGGKLDDFENMQTVYASLFWGIGLVMLLYLIYGVVQVYYMS
                     KFGQSIGKKLMKIRVVRENGDVAGFVHNVLLREFVYGLICSVIMMVLMGIFFAVFGLE
                     ANSGVAMVVDALLQIVSYLPTIICFIMLFMESRQRQTLQDMLAKTYVVQM"
     gene            complement(5360..5929)
                     /locus_tag="PK856_02645"
     CDS             complement(5360..5929)
                     /locus_tag="PK856_02645"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRL01303.1"
                     /translation="MTDLNNVYQSTQVDYEPEGADEMELASPGKRIAAYLINCLIGAV
                     AYIPMIWGAVSMSGSYAAAMDPENPVQMEPSGFAMGMIGLGSVLILAFLIFQAVLMSK
                     TGQSLGKRIMKIKVVNEDGDNPGFAGTVAMREIVPNLVLTVVGMIPFLGIIAQLGFWI
                     ACLVMLFLVDRDRRTLQDMIAKTYVVDAE"
     gene            6188..7309
                     /gene="asd"
                     /locus_tag="PK856_02650"
     CDS             6188..7309
                     /gene="asd"
                     /locus_tag="PK856_02650"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRL01304.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMQEEQDFKHIPESVFFTTSNIGGTA
                     PDFGQTEKTLLDANNITELAKMDIIVTCQGGDYTKAVFEPLRASGWNGYWIDAASSLR
                     MQDDAIIILDPVNRDVIDAGLKNGVKNFIGGNCTVSLMLVALGGLFQNDLVEWASSMT
                     YQAASGAGAKNMRELISGMGAIENEVAAELADPASAILDIDRKVSDFLRSDRFPDANF
                     GVPLAGSLIPWIDADLGNGQSKEEWKGGVETNKILGRSDNPTVIDGLCVRVGAMRCHS
                     QAITLKLKKDLSVAEIEALLAGANDWVKVIPNKKQASMEELTPAKVTGTLSVPVGRIR
                     KMGMGGEYITAFTVGDQLLWGAAEPLRRMLRIILGNLDQ"
     gene            7478..>7791
                     /locus_tag="PK856_02655"
     CDS             7478..>7791
                     /locus_tag="PK856_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HRL01305.1"
                     /translation="MRYLGWLFPAFFIAEIISIVLVSQWLGGWTLVLMALQFFAGLFL
                     LRNLGFSNVLVVGETLRNKQQGLSLYQLMWPIRFIVAGLLLLSPGFVSTVFAAGLMLP
                     LKA"
BASE COUNT         2019 a         2004 c         1737 g         2031 t
ORIGIN      
        1 agcgcacatg ctcaccgtct accaccaatt cacggctgcc cgatttggcc acggtgtctg
       61 tggtcatgat ggctttggcg gcaatgtccc aatgcacagg cttcatcttt ttgatggcag
      121 cctgtatttt ttcgtgtggc aacggctcca aaatcacgcc agttgaaaac ggcaacactt
      181 ggttggcttc gcacttgatt tgttcggcca cggcggcgca caccgcgcgc gcgcgtttca
      241 agccgtcttc gcctgtgccg gcattggcat tgcctgtgtt caccaccaaa gcacggatgt
      301 tggtgctcgg caacaactct tggcacacgc gcacaggcgc agcgcaatag cggttttggg
      361 tgaacacacc tgccacgcgt gaattcgggc ccaacaccat caaagtcacg tcgttgtaat
      421 ctggtttttt aattcccgct tggcccacaa acaattgcac cccatcaatg gtgagcaatt
      481 cttgggcatt tttaggcttc aagtgcacgg ccataacagc atcatccttt atcaagtgtt
      541 ggcttttttt gtaaactgcg tttataccac tttttgccct gtcaatcata gccaccccag
      601 ccatgacact tgccgcccca tccaagctga tgcagtatgt ggggcacgcc tcattccaat
      661 cagaaagtcc aaacatgagc atcatcgcca tagatgtaga cccacaacgc acgttcagcc
      721 ctttgtgtcc caatgaattg cccgtgcttg agggcgacca aattgtggat gaattgaatt
      781 tacaggccgc attggctagc aaacgcgtgc tcaccaaaga cgcacacaat cctcacgccg
      841 cttgggtagt ggccagccat gagcaaatcg gcacgcccat aggtctgcct aacagcgaca
      901 tcggttgggt tcgccatgcc gaagtcggca ccgatggttt caaagccttg cctggtttgc
      961 ctgagcctga acaatacgat ttcttggtct acaaaggtgt ggaaaacaac atgcaccctt
     1021 acggcgcgtg ttaccacgat ttgggtaaca ccatcagcac aggtttattg gaatggctga
     1081 cggtgaatgg cgctgaacat gtgttggtgg gcggcttggc caccgactat tgcgtgaaaa
     1141 acaccgtgtt gcaattgtgc gcttacaatc cgaattggac ggtgtggctg aacctcgctg
     1201 cctgccgagg cattgccgca gaaaccgtga tctcggcatt ggatgaaatg cgcacagcgg
     1261 gtgcgcacat cgtgaacaac agtgctgccc tgcgccaaca attggcttaa actgatctaa
     1321 ccttcagtgc tgtcattctg ttttgatgtt gaatgtgaac caaggagatg gacatgattt
     1381 accaaggaag ctgtcattgt ggtgccaccc gctacgaatt ggaagcggac cccattaccg
     1441 aagtcatcga atgcaattgc tcgcactgtc aacgcaaagg cttgttgctg tcttttcacc
     1501 cgcaaacaca gttgcgcatc cacagtggcg aagcccaact cacccaatat ttcttcaaca
     1561 aacacgtggt tcaacacatg ttctgccccg tttgtggttg cgaacctttt gccataggcc
     1621 aagaccccga gggttatgcg gttgctgcca tcaatgtgcg ttgcttggaa aacatcgatt
     1681 tggacgagct caaacgtcag ccgtttgatg gcaagtcagt ctgaacacca tgttccaatc
     1741 aaagtaacgt gtcaaaactt gttttaactg gagaatgaat acatgagtaa aaaaattctg
     1801 atgttggtcg gcgactatgc cgaagactac gaaaccatgg tgccgtttca gtttctactg
     1861 ggcgttggct accaagttga tgcggtgtgt ccagacaaaa aagcgggcga cagcatcaaa
     1921 accgccatcc acgactttga aggtgcgcag acttacagcg aaaaacctgg ccataatttt
     1981 gccctcaatg ccgactttgc caaggtcaat actgccgatt atgtgggctt ggtcatccct
     2041 ggtgggcgtg cgcccgagta tttgcgcttg aacccgcgtg tgctcgacat tgtgcgcgaa
     2101 tttcaccaag cgcaaaaacc catcgccgcc gtgtgtcacg gtgcgcaaat cttggctgcg
     2161 gctgaaatca tcgaaggcaa aaccgtgtct gcctaccctg cttgtgcgcc ggaagtgcgc
     2221 ttggcgggcg gtgtgtatgc cgagattgcc attgacgccg ctgtgaccga cgggcatttg
     2281 gtcaccgcac ctgcttggcc tgctcatccg cagtggctgg ctcaactcat cgcagctttg
     2341 ggtgcgaaaa tcgatttggg ttaaaagcca tgatttgctt ggtttaaacc ataaaaagcg
     2401 gcctgtaaac atttacagac cgcttttact cacacacctt tgtttattct gcactcaata
     2461 ccgcatcggt tttagcggca caaataaaat cattcttgtg caagcctgaa atggcgtgcg
     2521 tccaccacgt caccgtcaat ttgccccatt ccaataacaa ggcaggatgg tggccttctt
     2581 gttcggctaa ttcggccact ttgttgctcg ccgcccatgc ttgtttgaaa ttgcggaatg
     2641 tgtacacacg ctgcaattgc aacacgccat ccaccactat gggctgccaa tcggggattt
     2701 ggcgcaacaa ttcaggcaat tcctcatcgc tcacttgtgg cgcgcccgcg cggcaagcct
     2761 cacattgcat ttggtttaat tcatcactca tgtgtttctc catcatcaat tgtttttttg
     2821 agggtgcagc ccaagccatc accctgtctt aattaagcca gccatgcttt gctaaacacc
     2881 acgccacgca catgctgcat tttgttttcg cgagtaagtt gttcaaacac gggcaacacc
     2941 ccaccaaact gctgttcagt acataggctt aaactctgaa attttttgct gctggccaaa
     3001 aattggtcaa agaaatacgc atccacggtg gccacacaat ctgggtttcc caaggctact
     3061 tccaattttt gccccgtctc caaattatga aacaaacaat gttcgccatg cacatcaaac
     3121 caccatgcgc ctgacagtgt ttccccgcct gcatacacgt ccgcattttg gatttcatca
     3181 aaatacccgt ttgcgatgag agctttctca ggttggtcgg tttcggtgat gagtttgtgc
     3241 atcaactcat gtgccaatac tatgtgaaca tcaatggctt gcaacaaaac cgcttccatc
     3301 tccacatcca actgagtcat gccgattttc ccttaatgtg catcattatc aggcatgcac
     3361 gctaatgaat tttgacatta atgggcgatg gattcaggca atggaaacaa gcccagctct
     3421 cgcgctttcg ccacttcggc catgatgtcg gtattggcca aatcgtataa atcgttgacg
     3481 ctgttcaatt ggtagtaaat tggttgcaac acatcaatgc ggtagcgcgt gcgcagcaca
     3541 tcactgatat tgaactcacg ccgctcgggc acggctgaag catgcgcata ctcggtttct
     3601 tgtggcgaag acaagatgcc accgccgtaa attttcaaac cttgtgcggt attgagcaaa
     3661 ccaaactcta ccgtaaacca atacaaacgc gccaaatacg cccgctcttg cttgctcgcc
     3721 gccacaccca gtttgccata atgcgcggta aagtcggcaa aggcaggatg ggtaagcatg
     3781 gcacaatggc caaaaatttc atgaaaaata tcgggctctt gcaagtaatc catgtcttgt
     3841 ggacgacgaa taaacgtggc cacaggaaat ttgcgctcag acaagagctt aaaaaaacgg
     3901 tcgaagctga ttaaggccgg cacttcggca cattgccaac ccgtggcatc gtacagcacg
     3961 cggttgatgt cttttagggc aggaatgtgg tccagcggca aatccaagcg ctccaagcct
     4021 tgtaagtatt catcacaagc cttgtcttgc agattgccca attgacgcgt aatcagctga
     4081 tgccaagtgt ggtcttcggt ttcagaccaa tcgatgtggc cgtgttcatc gcttggtcga
     4141 gcggtgtaaa cgaatggctc atccataagg ctctcacttt gtattaattt caatgaatta
     4201 ttaattattg gatttattat ccataatttt tatatttcat gaatttatac aataaattat
     4261 ttcataaatc aacaagatta aaccctataa aacaccattc ccaagcatat tcatactcac
     4321 agaccacaca tctaagcaac aaatggcctt gggtcttcaa agacgatgcc cttaacacca
     4381 agttcccaac agcgcctcaa gcagggttca cacccttgcc ccatctcaat tcaagacccc
     4441 aaaacccatt gtatgaatta taaaaagcgg cctgtaaaca aatgttacag gccgcttcaa
     4501 taataaataa ggtgtcattt cgtgtttaca tttgcaccac ataggtttta gcaagcatgt
     4561 cttgcaaggt ttggcgctga cggctttcca tgaacaacat gatgaaacag atgatggttg
     4621 gaagatagct gacaatttgc agcaacgcat ccaccaccat ggcaacacca ctgttggctt
     4681 ccaagccaaa caccgcaaaa aagatgccca tcaacaccat catgatgaca gaacaaatca
     4741 aaccgtagac aaactcacgc agcaacacat tgtgcacgaa accagccaca tccccatttt
     4801 cgcgcaccac ccgtatcttc atcaatttct tgcctatgga ttgaccgaat ttgctcatgt
     4861 aatacacctg caccacccca taaatgaggt ataacagcat gaccagccct atgccccaaa
     4921 acaaagaggc ataaaccgtt tgcatgtttt caaagtcatc aagtttcccg ccgcttttaa
     4981 acaacagcac aatgatcaag ccaaatgcag gaatcatcaa caccacttca atcacacggt
     5041 taatgagcca cgccacaatg cgttgcaccg cagtcgccaa cgtcaactca ttgctgtcat
     5101 ctattgctgc agccacctcg acttgggtgg agtggtaaat attgtttaaa tcatccattc
     5161 aatgcccttt ttcacgaatc gtatttgata acccaatgcg aatcatctta agctgtattg
     5221 cagccagtat tttgtctgaa caagtcagct aatttcaatc aacacagtaa tcaggcttgt
     5281 gttgatacat tgtgattcat cttaaataac aaagcggcct gtaacgcttt acaggccgct
     5341 ttatggtcta gccaacaact tactcggcat ctaccacata ggttttggca atcatgtctt
     5401 gcaaggtacg gcgatcacgg tctaccaaga acaacatcac caaacacgca atccagaaac
     5461 ccaattgtgc aatgatgccc aaaaatggaa tcatgcccac aaccgtcaac accaaattgg
     5521 gaacgatttc acgcatcgcc acagtacccg caaagcctgg gttatcacca tcttcattca
     5581 ccactttgat tttcatgatg cgcttgccca aagattggcc tgttttactc atcaatacgg
     5641 cttgaaaaat caaaaaagcc aaaatcaaca cactgcccaa acctatcata cccattgcaa
     5701 aaccactcgg ttccatctgt actgggtttt ctgggtccat ggctgcggca tagctgcccg
     5761 acatgctcac cgcaccccaa atcatgggaa tgtaagcaac ggcaccaatc aaacagttaa
     5821 tcagatacgc ggcaatgcgt ttacctggcg aagccaattc catttcatca gcgccctcag
     5881 gctcgtaatc aacttgcgtc gattggtaaa cattgttcaa gtctgtcata tcccactcct
     5941 aaaaataaaa atgtatgtat ttatgctaaa aatgtatctc aaagacatta aaccttatac
     6001 tcaaattcgc acccatttta agttaatgca tgttaaatag caaaatttac tcaggtcctt
     6061 gaatcgaatt ttgtatctgc aacattttgt gcgctttttt tcgaaattat cctgtagtac
     6121 tgccaatttt tgggttatag ttttcacttc tattgatttt aagtcgtatc cgcaaaagga
     6181 gctcgttgtg aaagtaggtt ttgttggttg gcgcggcatg gttggttcgg ttttgatgca
     6241 acgcatgcaa gaagagcaag atttcaaaca cattcctgaa agtgtgtttt tcaccacctc
     6301 aaacattggt ggcactgcac ccgacttcgg tcaaaccgaa aagacattgc tcgatgccaa
     6361 caatattacc gaattggcca agatggacat catagtcact tgccaaggcg gcgattatac
     6421 caaagccgtg ttcgagcctt tgcgtgccag cggttggaat ggctattgga tagacgccgc
     6481 ttcaagcttg cgtatgcaag acgacgccat catcattttg gatccagtca atcgcgatgt
     6541 gattgatgcg ggcttgaaaa atggcgtgaa aaacttcata ggcggcaact gcaccgtgtc
     6601 tttgatgtta gtggctttgg gcggtttgtt ccaaaacgat ttggttgagt gggcgtcaag
     6661 catgacttac caagcggctt cgggcgcggg tgcgaaaaac atgcgtgaat tgatttcggg
     6721 catgggcgcg attgaaaacg aagtggcagc agaattggcc gaccctgcca gcgccatctt
     6781 ggacatagac cgcaaagtgt ctgacttctt gcgttcagac cgcttccctg atgcaaactt
     6841 tggcgtgcct ttggctggca gcttgattcc ttggattgat gccgacctag gcaatggtca
     6901 atccaaagaa gaatggaaag gcggcgtgga aaccaacaag attttgggcc gcagcgacaa
     6961 ccctactgta attgatggtt tatgcgtgcg cgtgggcgca atgcgttgcc acagccaagc
     7021 gattaccctg aaattgaaaa aagacttgtc ggtagccgaa attgaagcct tattggcggg
     7081 tgccaatgac tgggtaaaag tgatacccaa taaaaaacaa gcttctatgg aagaattgac
     7141 ccctgccaaa gtcactggca ctttatctgt accagttggc cgcatccgca aaatgggcat
     7201 gggcggcgaa tacatcacag ctttcaccgt gggcgaccaa ttgttgtggg gcgctgctga
     7261 gccactgcgt cgcatgctcc gcatcatctt gggcaacctc gatcaataag acagcttcgc
     7321 tacaaaaaag cctgaaccgc ttgggttcag gcttttttat tttatctcgt ccacatcgtc
     7381 tgcttggttt acttaatttg acttacaggc cgcttgaatt atgcccgtct tgcttatatg
     7441 ttgattaaac acttgataca caaatacagg aaaacacatg cgttaccttg gttggttgtt
     7501 tccggccttt ttcatagctg aaatcatttc catcgttttg gtcagccaat ggctgggtgg
     7561 ttggactttg gtgttgatgg cactgcaatt ctttgctggc ctgtttttac tgcgtaatct
     7621 gggcttttcc aatgtcttgg tggtgggtga aaccctgcgc aacaaacagc aaggtttgtc
     7681 gctctaccaa ttgatgtggc ccatccgctt catcgtcgca ggccttttgt tgctgagccc
     7741 tggctttgtg tccaccgtgt ttgccgctgg tttgatgttg cctttaaaag c
//
LOCUS       DAOMVK010000044         7785 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_2450_length_7785_cov_3.014360, whole genome shotgun sequence.
ACCESSION   DAOMVK010000044 DAOMVK010000000
VERSION     DAOMVK010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 7785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7785
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2450_length_7785_cov_3.014360"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="PK856_02660"
     CDS             complement(<1..681)
                     /locus_tag="PK856_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003743135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HRL01306.1"
                     /translation="MNIIKYLQAQGLGSRKQCQNWVKGGWIEIDGEIVDDTKAEVHLD
                     GRRVLKLDEDNIPTVDLPYFYILLNKAPDTETSHKPQQYPSVFSLLPNNFRDIDIQAV
                     GRLDADTTGAIILTNDGQFNHRVTSPKHHVAKIYRVTLKHAAEQSLCDKLIKGVLLDD
                     EHETIAAAAAELLDPNTLLMTLTQGKYHQVKRMIAAAGNRVEGLHRDQFHQWRVDDFA
                     VGEWRFLDV"
     gene            complement(748..2148)
                     /locus_tag="PK856_02665"
     CDS             complement(748..2148)
                     /locus_tag="PK856_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003772498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HRL01307.1"
                     /translation="MFTKLVTTALLTLPLSAQAAGYNPADMSLLWGLPFACILLSIAL
                     FPLFAPKIWHHSFGKITAMWTLLFLIPFAIVFGMGETVHTVAHAIIAEYIPFILLLWA
                     LYTVSGGILVKGNIHGSAKTNTIILALGTVLASLMGTTGAAMLLIRPLLRANDNRKHQ
                     VHVVVFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVEHMALPVLISSVILLAVF
                     YAIDSHYWGKETERKRVDPTPDSKISIAGKLNFVWLLGIIAAVLMSGIWKSGVEFDIL
                     GTPVTLQNIVRDVLFIIIGILSLKTTAQEIRKANDFDWGPILEVGKLFLGIFITIAPV
                     LAMLKVGMDGHFAPLVSLAHNAQGEPINAVYFWMSGMLSAFLDNAPTYLVFFNLASGD
                     AVDLMSHMSSTLLAVSMGSVFMGALSYIGNAPNFMVKAIAEQNRVKMPSFFGYMAWSF
                     GILVPLFIIHTLIFFI"
     gene            complement(2264..2659)
                     /gene="gloA"
                     /locus_tag="PK856_02670"
     CDS             complement(2264..2659)
                     /gene="gloA"
                     /locus_tag="PK856_02670"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HRL01308.1"
                     /translation="MRLLHTMLRVGDLDRSVAFYQEVLGMKLLRKNDYPEGKFTLAFM
                     GYGDESDHTVLELTHNWDTSSYDLGNAYGHIAIEVEDAYAACDAARAKGGNVVREAGP
                     MKHGTTVIAFVEDPDGYKIEFIQRNSGQD"
     gene            complement(2798..3490)
                     /locus_tag="PK856_02675"
     CDS             complement(2798..3490)
                     /locus_tag="PK856_02675"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HRL01309.1"
                     /translation="MITWQFEDDLGVCHVRVNQRGKTQIRLRVKERDLIEISAPKHLT
                     AKRLHSFLAEHQAWLYQQRSQLTSDTQPEHIWYQGRQLPILMRPVNTEIEFQSDACVV
                     SSSLERPYRSLLQHLQQHAHKQLLPRLKTWLPTQHANTDLRLSNAQSYWGICRPQQIK
                     LNWRLIGAPSAVMDYVCVHEMCHLNHPNHSAAFWQAVAEHMPDYRTHRLWLKQHGSAI
                     MQSCKLLVQHSQ"
     gene            complement(3487..4251)
                     /locus_tag="PK856_02680"
     CDS             complement(3487..4251)
                     /locus_tag="PK856_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRL01310.1"
                     /translation="MTLLKNLLYWLCVSLFTVVYFWYIAVCLAFGVKAANRACTNWAM
                     TLLWLLEHIIGLKYEVKGLDNIPDEPVVICCKHQSGWETLILQKFFRPIVFVAKKELF
                     KIPFFGWGLKLAGTIGIDRKDRLGASRQLLEVGGLRKKQGFSIAIFPEGTRIKPGLRG
                     RYKLGGARIANTLQMPIVPVALNSGEFWPKNSFMKYPGTISVVIGKPIHPSEQGPEAV
                     MQQVETWIESQQRVIEGRGPFANKEVAAEQDERTGS"
     gene            complement(4254..4835)
                     /gene="gmhB"
                     /locus_tag="PK856_02685"
     CDS             complement(4254..4835)
                     /gene="gmhB"
                     /locus_tag="PK856_02685"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HRL01311.1"
                     /translation="MTKLVILDRDGVINEDRDDFVKSVDEWVALPGSMDAIAFLNQAD
                     FRVVVATNQSGIGRGYFTTHDLNEMHTKMHRLVQQAGGQIEAIWFCPHTAADKCECRK
                     PLPGMVEDILQRYLNTDPDELYMVGDSLRDLQAIEAVGGQPVLVLTGKGEKTLRDPAL
                     PEDTIVYHDLLEFAQAMLHNHQKRQAAQAEQEE"
     gene            complement(4832..5293)
                     /locus_tag="PK856_02690"
     CDS             complement(4832..5293)
                     /locus_tag="PK856_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HRL01312.1"
                     /translation="MEFEIIDHQEWHTEEDFPADLNPVHALTHMGMYFEWAAHKQLLH
                     PDVAADLAPTLKKLDNREVTGRDIVEQALGKQLLLSFFNDVGQRFTAYYYADDDEGYG
                     QFLSDYVTTLRTDLLPSFYHVDNSQAAYDAIASQFDIAYARWSDSLRTTAS"
     gene            5726..7108
                     /gene="dbpA"
                     /locus_tag="PK856_02695"
     CDS             5726..7108
                     /gene="dbpA"
                     /locus_tag="PK856_02695"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HRL01313.1"
                     /translation="MSNTPFSSLPLPQALLSNLTDLGYEAMTSVQAQALPEVLAGKDV
                     IAQAKTGSGKTATFGLGLLAQLDVKYFRVQSLILCPTRELADQVATELRKLARSIHNI
                     KILTLCGGAPMRPQITSLEHGAHIIVGTPGRVMDHLDQGRLSLDDLRCLVLDEADRML
                     DMGFQTELDSVIAKAPLKRQTLLFSATYPDSIAALSARLLNKPVHIKVEEQHGNSSIA
                     QKFFQVNDNRDRMNALRLLLLKYQGQSSIVFCTTKRETQEVADLLTSYGFSVLALHGD
                     LDQKERDQALVKFANGSACVLVATDVAARGLDISSVDAVFNYQLAHDADTHTHRIGRT
                     GRAGQQGMALSLYADSDALRMIALEEGLGLKIEAQPLPDESVLQTKPTKPAMATLQLD
                     SGKKNKLRPGDIVGALTASKQLTGDDLGKIQVYDIWSFVAVKRSAAGLALQMLQDSKI
                     KGKSVRARVL"
BASE COUNT         1894 a         1746 c         2092 g         2053 t
ORIGIN      
        1 cacatccaag aagcgccatt cgcccacggc gaagtcgtcg acgcgccatt ggtggaattg
       61 gtcgcggtgc aagccttcga cgcggttgcc tgcggcggca atcatgcgtt tgacttggtg
      121 gtatttgcct tgggtcagcg tcatcaataa ggtgttgggg tcgagcaatt cggctgcggc
      181 tgcggcgatg gtttcgtgtt cgtcatcgag caacacgcct ttgatgagtt tgtcgcacag
      241 gctttgctcg gccgcgtgtt ttaaggtgac gcggtagatt ttggccacgt ggtgtttggg
      301 cgaggtcacg cggtggttaa actggccatc gttggtgagg atgatggcgc cggtggtgtc
      361 ggcatctaag cggccgacgg cttggatgtc gatgtcacga aaattattgg gcagcaagga
      421 aaacacgctt gggtattgct gcggcttgtg cgaggtttcg gtgtcgggcg ctttgttgag
      481 caagatgtag aaatacggca aatccacggt ggggatgttg tcttcatcga gtttcaacac
      541 gcggcggcca tcgagatgga cttcggcttt ggtgtcgtca acgatttcgc catcgatttc
      601 tatccagcca cctttgaccc agttttgaca ttgtttgcga ctgcccaaac cttgggcttg
      661 taagtatttg atgatgttca tatgcattct aaacaaaagc ggcctgtaaa catttacagg
      721 ccgcttgatt gattgtaaac gcgcttatta tataaagaaa atcagcgtgt gtatgataaa
      781 caagggcacc aagatgccaa acgaccatgc catatagcca aaaaagctcg gcattttcac
      841 gcggttttgc tcggcgatgg ctttgaccat gaagttgggt gcgttgccaa tatagctcaa
      901 agcgcccatg aacaccgagc ccatggacac ggcgagcaag gtagacgaca tgtggctcat
      961 caaatctacc gcatcgcctg aggccaaatt gaagaacacc aaataggttg gcgcattgtc
     1021 caagaaggcc gacaacatgc ccgacatcca gaaatacacc gcattgatgg gctcaccttg
     1081 ggcattgtgt gccaagctca ccaaaggcgc gaaatggccg tccatgccca cttttaacat
     1141 cgccaacaca ggtgcgatgg tgatgaagat gcccaagaac aattttccca cttccaaaat
     1201 cgggccccag tcaaaatcgt tggccttgcg gatttcttgt gcggtggttt tcaaggacaa
     1261 gatgccgatg atgatgaaca acacatcgcg tacgatgttt tgcaaggtga caggcgtgcc
     1321 caagatgtca aattctaccc ctgacttcca aatgccagac atcaataccg cggcgatgat
     1381 gccgagcagc cacacaaaat tgagcttgcc tgcaatgctg attttgctgt caggcgtagg
     1441 gtctacccgt ttgcgttcgg tttctttgcc ccagtaatgg ctgtctatgg catagaacac
     1501 cgccaataaa atcaccgagc tgatgagcac aggcaaggcc atgtgttcca ctgtccacat
     1561 gaagtccacg ccttttaaga agcctaagaa caagggtggg tcgcccaatg gggtcaagcc
     1621 gccgccgatg ttggccacca agaagatgaa gaaaaccacc acatgcactt ggtgtttgcg
     1681 attgtcattg gcgcgcaaaa gtgggcgtat taacaacatc gccgcgcctg tggtacccat
     1741 taatgaggcg agcaccgtgc ccaaggccaa gataatggta ttggttttgg cgctgccgtg
     1801 gatattgcct ttgaccaaaa tgccgccaga cacggtgtac aaggcccaca gcagcaaaat
     1861 aaatggaatg tattcggcaa tgatggcatg ggccacggtg tgcacggttt cacccatgcc
     1921 gaacacaatg gcaaagggaa tcaaaaacag cagcgtccac atggcggtga ttttgccaaa
     1981 actgtggtgc cagattttgg gggcaaacaa gggaaataag gctatggaaa gcaggataca
     2041 ggcaaaaggc aagccccaca gcaagctcat gtctgcaggg ttgtatcctg cggcttgggc
     2101 gctgaggggc aaagtgagta atgccgtggt gaccagtttg gtaaacatgg acgtcctttc
     2161 gtggttgcaa tgatgggccg cagcattgta tttgtgtgtg acgagggctg cgtgcgccaa
     2221 aaagggcgac cgcttggcat gagcgcaagc ggtttaaaac caatcagtct tggcctgagt
     2281 ttctttggat gaattcgatt ttgtagccat ctgggtcttc gacaaaggca atgacggtgg
     2341 tgccgtgctt catcgggcct gcttcgcgca ccacattgcc acctttggcg cgggcggcat
     2401 cacaagcggc ataggcatct tctacctcaa tggcgatgtg gccataggca ttgcccaagt
     2461 catagctgct ggtgtcccaa ttgtgggtga gttccaaaac cgtgtggtcg ctttcatcgc
     2521 catatcccat aaaagccaag gtgaatttgc cttctggata atcgtttttg cgcaataatt
     2581 tcatgcccaa cacttcttgg tagaacgcca ctgagcggtc taaatcgccc acgcgcaaca
     2641 tggtgtgtaa cagtcgcata acttggcctt ttgatggttt gatatttgta aggcatgaat
     2701 aataggcgac attgggtggg tttgccaatg ctgcgatgca gcattattcg gtattttgtg
     2761 ccatgattga tggctttttt aaaatactgt tttagaatta ttgtgaatgc tgcaccaata
     2821 gtttgcacga ttgcatgatg gcagagccat gttgtttgag ccacaaacga tgtgtgcggt
     2881 agtctggcat gtgttcggcc accgcttgcc aaaatgctgc cgaatgattg gggtggttta
     2941 agtggcacat ttcatggaca cacacataat ccatcaccgc actcggtgca ccgattaagc
     3001 gccagttaag cttgatttgc tgcggccgac aaatgcccca atagctttgg gcattggaca
     3061 agcgcaagtc ggtgttggcg tgttgtgtgg gaagccaagt tttgaggcgg ggtaagagtt
     3121 gcttgtgggc gtgttgctgc aggtgttgca agagggagcg gtaagggcgt tccaagcttg
     3181 aactcaccac acaggcatcg ctttgaaatt caatttctgt atttacaggc cgcatgagta
     3241 tggggagttg ccgaccttgg taccaaatgt gttcgggttg ggtatcactg gtcagttggg
     3301 agcgttgctg ataaagccaa gcttggtgtt cggcaagaaa agaatgtaag cgcttggctg
     3361 tcaggtgttt gggtgcgctg atttcaatca ggtcacgctc tttcaccctg agcctgattt
     3421 gggttttgcc gcgttgattg acacgcacgt ggcacacacc caaatcgtct tcaaattgcc
     3481 aagtgatcat gagccggtgc gttcatcttg ttcggctgcg acttctttgt tggcaaatgg
     3541 gccacggcct tcaatgacac gttgttggct ttcaatccaa gtttccactt gttgcatcac
     3601 cgcttcaggg ccttgttcgc ttgggtgaat cggcttgcct atgaccacac tgatggtgcc
     3661 tgggtatttc atgaacgagt ttttcggcca aaattcacca ctgttaagcg ccacgggcac
     3721 gatgggcatt tgcaaggtgt tggctatgcg tgcgccaccc aatttgtagc gaccgcgcaa
     3781 accaggcttg atgcgcgtgc cttctgggaa gatggcaatc gagaagcctt gcttcttgcg
     3841 caagccaccc acttccaaca attggcgcga tgcgcccaag cggtctttgc ggtctatgcc
     3901 tatggtgccc gccagtttca agccccaacc aaaaaagggg attttgaaca attctttttt
     3961 ggccacaaac acaatcgggc ggaagaattt ttgcaagatg agggtttccc agcccgattg
     4021 gtgtttgcaa caaatcacca caggttcatc gggaatgttg tccaaacctt tgacttcgta
     4081 tttcaggccg atgatgtgtt ccaataacca cagcaaagtc atcgcccaat tggtgcatgc
     4141 gcggttggcg gctttcaccc caaaggccaa acacactgcg atgtaccaaa aatacaccac
     4201 ggtaaacagg ctgacgcaca gccaatacag taaatttttc agtaaagtca tttttattct
     4261 tcttgctctg cttgtgccgc ttggcgtttt tgatggttgt gtaacatggc ttgtgcaaat
     4321 tctaacaaat catggtagac aatggtgtct tctggtaaag cagggtcacg caaggttttc
     4381 tcgcccttgc cagttaaaac caacacaggt tggccaccca cagcctctat cgcttgcaag
     4441 tcgcgcaagc tgtcgcccac catatacaat tcgtctgggt cggtgttcaa atagcgctgc
     4501 aaaatgtcct caaccatgcc tggcagcggt ttgcggcatt cgcatttgtc ggccgctgtg
     4561 tgcgggcaaa accatattgc ttcgatttga ccgcccgctt gctgtaccaa gcggtgcatt
     4621 ttggtgtgca tctcattcaa atcgtgggtg gtaaaatagc cgcgccctat gcccgattga
     4681 ttggtcgcca ccaccacccg aaaatcggct tgattcaaga atgcaatcgc atccatgctg
     4741 ccaggcaagg ccacccactc atccaccgat ttaacgaaat cgtcgcggtc ttcattgatg
     4801 accccatcgc ggtctaaaat caccaatttg gtcatgatgc agtggtcctt aaactgtcgc
     4861 tccagcgtgc ataagcaatg tcaaattgag aggctatggc atcgtaggcc gcttggctgt
     4921 tgtccacatg gtaaaacgag ggcaataaat cggtgcgtaa ggtggtgaca taatcgctca
     4981 aaaactgacc ataaccctcg tcatcatccg cataataata agcggtaaag cgctggccga
     5041 catcattaaa aaagctcagc aacaattgtt tgcccaaggc ttgttccaca atgtcgcggc
     5101 ctgtcacttc gcggttgtcc agtttcttca aggttggtgc caaatcggct gccacatcag
     5161 ggtgcagcaa ttgcttgtgc gcggcccatt caaaatacat gcccatgtgg gtcaatgcgt
     5221 gtactgggtt caaatccgcg ggaaaatctt cctcagtatg ccattcttgg tggtcgatga
     5281 tttcaaattc catattcggc tcagctgcag tcggaaaagg acaaaactat aacattgtgt
     5341 agagcacatg cacatagctg tcgacagctt tgcttgggta tcttctccat ggcgtgtgtt
     5401 tgatgatttg attactgact ttatttatat gtgtgtacac atgatgtgca ggcactgatt
     5461 gagtgtgcct aaattggtga agtggcttta caggctgctt gttcagaatg ttttgcgaca
     5521 tgtgatgcag cagctacata taaattgcac catgctaaag aagaggcttg gacaatattg
     5581 gcagtgaata cataatcttg tgcgttgatg cggcctgtaa aaattaacgg gcgcacgaat
     5641 gatggtcagg cgattctgtt acaatgctcg gttttgtgat ttcgggtgtg gctttagccg
     5701 caccaaacga cagtggagca gtaacatgag caataccccg ttttccagcc tgcctttgcc
     5761 gcaagcctta ttaagcaatt tgaccgattt gggctatgaa gctatgacca gtgtgcaagc
     5821 acaagccttg ccagaggtat tggcgggtaa agatgtgatt gcgcaggcaa aaacgggctc
     5881 gggcaaaacc gccactttcg gcttgggttt gttggcacag ttggatgtga agtatttccg
     5941 tgtgcaaagc ctgattttgt gcccaacacg cgagttggca gaccaagtgg ctaccgaatt
     6001 gcgcaaattg gcgcgcagca tccacaacat caaaatttta actttatgtg gcggcgcacc
     6061 gatgcgacca caaatcacct cgttggaaca tggcgcacac atcattgtgg gcacgccagg
     6121 tcgggtgatg gatcatttgg atcaaggccg tttgagctta gacgatttgc gctgcttggt
     6181 attggacgaa gccgatcgca tgttggacat gggttttcaa accgaattgg acagcgtcat
     6241 cgccaaagca ccgttaaaac gccaaacctt attgttcagc gcgacttatc ctgattcgat
     6301 tgcggctttg agtgcgcgct tgttgaacaa acctgtgcac atcaaagtag aagagcaaca
     6361 tggcaacagc agcatagcgc aaaaattttt ccaagtgaac gacaatcgcg atcgcatgaa
     6421 tgccttgcgt ttattgctgt tgaaatacca aggccaaagc agcatcgtgt tttgcaccac
     6481 caagcgtgaa acccaagaag tggccgattt gctcacttca tacggtttca gtgtgttggc
     6541 tttgcacggt gatttggatc aaaaagagcg cgatcaagcc ttggtgaaat ttgccaatgg
     6601 cagtgcttgt gtattggtcg caaccgatgt ggcagcgcgt ggcttggaca tcagcagtgt
     6661 tgatgcggtt tttaattatc aattggccca cgatgccgat acccacaccc accgcattgg
     6721 ccgtacaggt cgcgcaggtc agcaaggcat ggctttgagc ctgtacgcgg acagcgatgc
     6781 cttgcgcatg attgccttgg aagaaggctt gggtttgaaa atagaagccc agcctttgcc
     6841 tgatgagtcg gtattgcaaa ccaagcccac caagcccgct atggcgactt tgcaattgga
     6901 cagtggcaaa aagaacaagc tgcgccctgg tgacattgtt ggagcattga ccgccagcaa
     6961 acaattgacg ggcgatgatt tgggtaaaat tcaggtctac gacatctgga gttttgttgc
     7021 agttaagcgt tcagctgcgg gtttggcttt gcaaatgctg caagacagta agattaaagg
     7081 caagtcagtg cgcgcacgcg tgttgtaagt ggtattgaat taaaacattt gtcagtatga
     7141 atgtgattga ataaaagcgg cctgtaaata agtttacagg ccgcttttat tgggattttt
     7201 caaatctcac tattaatgtg taatgtgtgt tgtgatgaga tgagatgaga tgagatgaga
     7261 tgagatgaga tggataaagc tatggctaat tgggtgtgac tgcatatgta tggctatata
     7321 gattgatagg agtatcggta tttggaaagt gggcagtgaa ggtttgagat acagggcttc
     7381 aatgcagaaa tatctatata tagattgtat tttgcaaatt tgctcagagt gattgttgta
     7441 tccactttgt aagcggcctg taaggttgaa atgcttggta atgggttctg aatgatggaa
     7501 tttgtgagtt gttggaaacg gtaatggatt atctataata tgtcttgtgt attaaagtgg
     7561 gattatccat aaagcggcct gtaaatgtgt attgcacgat ttacaggccg ctttggtgtt
     7621 tgggtacgct gtttttatgt gttttcaaaa agggttttgg atggttttgg gttgtctgaa
     7681 tgggtttggc tggtggtgac tggagagaaa tgcaggtgta gcgagcaagt ttttgtgggg
     7741 atggtgatga atttcagcca atttgtagat ttttgaggtt ttctt
//
LOCUS       DAOMVK010000045         7735 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_2468_length_7735_cov_2.653125, whole genome shotgun sequence.
ACCESSION   DAOMVK010000045 DAOMVK010000000
VERSION     DAOMVK010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 7735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7735
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2468_length_7735_cov_2.653125"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PK856_02700"
     CDS             <1..403
                     /locus_tag="PK856_02700"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003824962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRL01314.1"
                     /translation="DFVAADHLPLVVYFYPKDSTPGCTTEAQGFRDSIAAFKAAGYTV
                     VGISRDSVKSHQNFVCKQELNFPLLSDKEETVCTQFDVIKEKNMYGKKVMGIERSTFV
                     LDANGDLLKEWRKVKVAGHVDEVLAFVQQPA"
     gene            complement(582..>772)
                     /locus_tag="PK856_02705"
     CDS             complement(582..>772)
                     /locus_tag="PK856_02705"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRL01315.1"
                     /translation="GLSFARWRQQVQLLHALLQLAAGKSVQQVSMDLGYESVSAFITV
                     FKKRLGTTPKRYFSAEPV"
     assembly_gap    773..782
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<783..1403)
                     /locus_tag="PK856_02710"
     CDS             complement(<783..1403)
                     /locus_tag="PK856_02710"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HRL01316.1"
                     /translation="MVDLLQHLNQTHQEAQEQRISVTRVTADACDYESPQHEHAQGQL
                     VMALAGGVRCAIQGQVWMVPPQGAVWIPPHQTHTNYVTHNAEVCIAFIATAAGLPEVP
                     VGTIAVSPLLRELLLQLAGLQVASIATLTPPTSALMTEDVYQQCLVDLLLLELSRAQP
                     QAYAIPVLHHPRLQLLYQSLLAEPQNRLGSSAWAERLAMSERTLLRL"
     gene            1501..2673
                     /locus_tag="PK856_02715"
     CDS             1501..2673
                     /locus_tag="PK856_02715"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL01317.1"
                     /translation="MKSNSVPWLLFGVLLVASNLRLPITSIGPVIQNIGADWQLGSSA
                     LGLLTTLPLLAFALISPLTPPLAQRFGLNRTLAGCLVLIILGFTGRLLPSLPMLYIGT
                     ALIGAGIAIGNVLLPSLVKAHFPEKIALITSVYAFTMGLAAAAGSALVIPLMQSFAWT
                     WAQALFAFVGLTIISLLVWLPQLKQQAVHTAVTNQSVKSTEKAVWRYGLAWEVAAFLG
                     MNSLIFYSIITWLPSMLQAAGYSASAAGSIHGLLQLFSALPGFVIVPLMARFQDQVIP
                     ATLVGLCTLLGLLGLNLWPEAAHVWAAMIGFGSGGTIILGLMFVGLRTHTPERAAALS
                     GMAQSLGYLLAAVAPPLLGLLQQAQHHWHSVLWILSAAAIIVLINGLLSGRNRYIP"
     gene            2856..4253
                     /locus_tag="PK856_02720"
     CDS             2856..4253
                     /locus_tag="PK856_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRL01318.1"
                     /translation="MTTKKLFVLDTNVLLHDPQSLFRFDEHDIYIPLITLEEMDAHKV
                     GMTDVARNARQASRYIDELFSKEPDSLTSGIPLNLAGHKEARGKLILQTSVETASLPD
                     SLLGEKADNLILGLVKYLQEQKNGYEHVALVSKDINIRIKARALGLYAEDYFNDKVLE
                     DSDVLYTGYRQLDGEFWLRNGSELQSWQEQGSVFYRIQGEDALQMLPNELLLDEDGEK
                     TLQARVIEVNRTEKVATLQTVKDYTHPKNSVFGITARNWGQNYALNLLMDPEIDLVTL
                     LGQAGTGKTLLTLASALTQVLDQQRYSEIIMTRVTVPLGNDIGFLPGTEEEKMNPWMG
                     ALEDNLEVLTHSDDMGGDWARAATHDMVASKIKIKSINFMRGRTFQHKFLIIDEAQNL
                     TSKQMKTMITRAGEGTKIVCMGNLAQIDTPYLSEGSSGLTYLVDRFKNWPHFGHITMQ
                     HGERSRLAAHAVAVL"
     gene            4645..6471
                     /gene="dsbD"
                     /locus_tag="PK856_02725"
     CDS             4645..6471
                     /gene="dsbD"
                     /locus_tag="PK856_02725"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003746482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HRL01319.1"
                     /translation="MALKQSVVYFLALCVVFFSNLAHAAIDPNSILPAEEAYKTQLFA
                     TEDGVSLQFTIADGYYLYKDKLEFNSDGPSLGKPEFSPAQSTTDEFFGTQAVYFKVAQ
                     INIPFSKAHNPAQPYRFHLNYQGCAEVGICYPPTKVEFAVDKVSLIAPLASGKINVFS
                     NSSTPNFGSPSNKTATTSPTSSQTASNSSTGFNLNQDAVWRSLLTFFIAGIGLSLTAC
                     MYPLLPIVSSIVVGNKRSKRIAFWLSLLYVQGLALTYTVVGVITGLTGSLLTVWLQQA
                     WVVLAAAALLVVLALAMFDVIQIQLPNRIQSYFQQQSSRMSGGKLASVFGMGMLSALI
                     VGPCVAPPLAFALGYIAQSGNAVLGGAALYAMAMGTGLPLILVATFGAHVLPKAGTWM
                     NSIKVVFGCLLLAAAVYMARPFLPYWLTISLYAAILFGLGLYLITRSFKVVSKWKYLP
                     LTLGAALLAVSAYFSGETVRGNTTPLHQALSLQAPSQAHFGRQYTDVQALQAAMNEAL
                     AQDPSRPVILDFYADWCVTCIQMQMNTFNTDTVKSQVDEQRFFTIDVTANTAEHQALL
                     KEYGLFGPPGIFKINHDGKRSAAVLGFVGADEFVKWVKEPLP"
     gene            complement(6487..7461)
                     /locus_tag="PK856_02730"
     CDS             complement(6487..7461)
                     /locus_tag="PK856_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01320.1"
                     /translation="MGIGYENYVFWCFMALVFMRVVLYVFTPKLCSHQRKCGYLIRDV
                     LMLLLAYQVCGILNDFQWRYDDQDLNWLVLWAILMGIFAWSFADFLRMFNSRLALKRV
                     ALPKPHKGTIKFKSKPFNPVDSAYVSVHLLVQMMLYTVLLGFWIFLGASVWGYVQPMQ
                     KFSAQKQVVEYTLEEDVALKLLRYRDGTFRGMKFHTEGGEYVFMDITTPESIWRKTLP
                     DLFRVYPGSEKAFFSSTFVEQLHFYSVNDIQSSVLVSKMKGGMRHREMVYSVDPKLLN
                     QYVGQTNPLPPKHTHGVALLLFVFLAAGITIKLVKKIRQVMVYDLENA"
BASE COUNT         1911 a         2016 c         1828 g         1970 t
ORIGIN      
        1 agattttgtc gctgccgacc acttgccttt ggtggtgtat ttctacccca aagattccac
       61 cccaggctgc accaccgaag cccaaggctt tcgtgacagc attgctgcct tcaaagccgc
      121 aggttatacc gtggtgggca tttcacgcga cagcgtgaaa tcgcaccaaa attttgtctg
      181 caaacaagaa ttgaatttcc ctttgctctc cgacaaagaa gaaaccgtgt gcacgcaatt
      241 tgatgtcatc aaagaaaaaa acatgtatgg caaaaaagtg atgggcatag aacgctccac
      301 atttgtgcta gatgccaacg gcgacttgct caaagaatgg cgcaaagtca aagtggcagg
      361 ccatgtggat gaagtcttgg cttttgtaca acagcctgct tgattgctgg ttttcacatt
      421 tctctcacgc tgcctgagcc tcaaaactcg cagcgtgttt ttcaatgcaa aacaggctta
      481 caggccgcat caaaacctgt tttcaagctt tcattgattt tttctacgcg atgttcccaa
      541 taccagcagg ccatttacag gccgctttaa tgggtattac atcacacggg ctcagcagaa
      601 aaatagcgtt tgggcgtggt gcccaagcgt tttttaaaca cggtaataaa cgcgctcacc
      661 gactcatagc ccaaatccat cgacacttgt tgcacgcttt tgcccgcggc caattgcagc
      721 aaggcatgca aaagctgcac ttgctgtcgc cacctcgcga aactcaaacc cgnnnnnnnn
      781 nncaagcgca ataaggtgcg ctcactcatg gccaagcgtt cggcccatgc gctgctgccc
      841 agccgatttt gtggttctgc cagcaagctt tggtaaagca attgcaaacg cggatggtgt
      901 aacacgggta tggcataggc ttgcggctgc gcccgcgaca actccaataa caataaatcc
      961 accaaacact gttggtacac atcttccgtc atcagtgcgg atgttggcgg tgttaaggtt
     1021 gcgatagaag cgacttgcaa acctgccagc tgcagtagca attctcgtaa caaaggcgac
     1081 acagcgattg tgcccacggg cacttcaggc aaacccgctg ccgtggcaat gaatgcgatg
     1141 cacacttcgg cattgtgggt gacataattg gtgtgggttt ggtgcggcgg tatccacaca
     1201 gccccttgcg gcggcaccat ccagacctga ccttgtatgg cgcagcgcac accaccagcc
     1261 aaagccatga ccaattgccc ttgggcgtgt tcgtgttgtg gcgactcata atcacaagcg
     1321 tcggccgtga cccgagtcac gctgatgcgc tgttcttggg cttcttgatg ggtttggttg
     1381 aggtgttgga gcaagtcgac catattgtct gatttcagtg attttttggc attatagcta
     1441 aatttttcct aatcacgatg tattacactg tcggcctgta ccctaacaag tgattccatc
     1501 atgaaatcga attccgttcc atggctattg ttcggcgtgt tgttggttgc cagcaatttg
     1561 cgtctgccca tcaccagcat aggccctgtg atacaaaaca ttggcgctga ttggcaattg
     1621 ggcagctcgg ccttgggttt gctcaccacc ttgcccttgc ttgcctttgc cttgatttcc
     1681 cctttaaccc cgccattggc acaacgcttc gggctcaacc gcaccttagc gggctgtttg
     1741 gtgctcatca tactcgggtt tacaggccgc ttattgccct ctttacccat gctctacatc
     1801 ggcacggcct tgataggcgc aggcatcgcc attggcaatg tgttgttgcc cagcttggtg
     1861 aaagcgcatt ttcctgaaaa aattgcgctg attaccagcg tgtacgcgtt taccatgggc
     1921 ttggcggcgg cagcaggctc ggctttagtg attccattga tgcagtcgtt tgcatggact
     1981 tgggcacagg cgctgtttgc ttttgtcggc cttaccatca tcagcttgtt agtgtggtta
     2041 cctcaattga agcaacaagc cgttcacaca gctgtcacaa atcagtctgt aaagtctact
     2101 gaaaaagccg tgtggcgcta tggtttggct tgggaagtgg cggcgttttt gggcatgaat
     2161 tcgttgattt tttacagcat cattacttgg ttaccatcca tgttacaggc cgcaggttac
     2221 agcgccagtg ccgcaggctc aatccatgga ttgttacaat tgttttccgc cttgcctggc
     2281 tttgtcatcg tgcctttgat ggcgcgcttt caagaccaag tcatccctgc gactttggtg
     2341 ggtttgtgca ctttgctagg cttgctgggc ttgaatctgt ggcctgaggc ggcacatgtg
     2401 tgggcggcca tgataggttt tggttcaggc ggcaccatta ttttgggctt gatgtttgtg
     2461 ggtttgcgca cccacacacc cgaacgggca gcggccttat cgggcatggc gcaaagcttg
     2521 ggctatttat tggcagccgt tgcgcccccg ttattgggtt tattacaaca agcccaacac
     2581 cactggcact cggttttgtg gattttaagt gcggcggcaa tcatcgtatt aatcaatggc
     2641 ttactctcag gacgcaatcg ctacatccct tagtcacgtt actgtcatac aaggattcta
     2701 tgctgtgttg gcattgacag ggtttgccaa tcacagcaca taggctcaca ctgtcaataa
     2761 ggcacttctg taaccaattg accatcaagc ttacaggccg catcgctgag cttgctacct
     2821 aaggatgaca ttcctgtcac aagtggaggg tttacatgac gaccaagaaa ttatttgtgc
     2881 tcgataccaa tgtattgtta cacgacccac aaagcctgtt tcgattcgac gagcacgaca
     2941 tctacattcc cctcatcact ttagaagaaa tggacgccca caaagtgggc atgacagacg
     3001 tggcacgcaa tgcccgtcaa gccagccgtt acatagacga gttgttcagc aaagaacccg
     3061 attcactcac ctctggcatt cctctcaatc tcgccggcca taaagaagcg cgtggcaaac
     3121 tcattttgca aacctcggta gaaaccgcct ctttgcccga ttctttattg ggcgaaaaag
     3181 ccgacaattt gattttgggc ttggtgaaat acctgcaaga gcaaaagaat ggctatgaac
     3241 atgtggcttt ggtgtcgaaa gacatcaaca tccgcatcaa agcccgtgcc ttgggtttgt
     3301 atgccgaaga ttatttcaac gacaaagtgt tggaagacag cgacgtactt tacactggct
     3361 accgccaatt agacggcgaa ttctggttgc gcaatggcag tgaattacaa tcgtggcaag
     3421 agcaaggctc ggtgttttac cgcatccaag gcgaagatgc cttgcaaatg ctgcccaatg
     3481 aattgctttt agacgaagac ggcgaaaaaa ccctgcaagc acgggtgata gaggtcaacc
     3541 gcactgaaaa agtggccacc ttacaaaccg tcaaagacta tacccatcct aaaaacagcg
     3601 tgtttggcat caccgcccgc aattgggggc aaaactacgc cttaaacctg ttaatggacc
     3661 ctgaaatcga cttggtgacc ttgcttggtc aagcaggcac aggcaaaacc ttgctcacct
     3721 tggcctcagc cttaacccaa gtactggacc agcagcgcta cagcgaaatc atcatgaccc
     3781 gcgtcaccgt gcctctgggc aacgacatcg gcttcttgcc tggcacagaa gaggaaaaaa
     3841 tgaatccgtg gatgggcgca ctcgaagaca atttggaagt gctcacccac agcgatgaca
     3901 tgggcggcga ttgggcgcgc gcagccaccc acgacatggt ggcgagcaaa atcaaaatca
     3961 aatcgattaa cttcatgcgc ggccgcacct tccagcacaa atttctcatc atagacgagg
     4021 cgcaaaacct cacctcaaaa caaatgaaaa ccatgattac ccgcgcaggc gaaggcacaa
     4081 aaattgtgtg catgggcaat ttggcccaaa tcgacacgcc gtatttaagc gaaggctcaa
     4141 gcggcctgac ctatttggta gaccgattca aaaactggcc gcactttggc cacatcacca
     4201 tgcaacacgg cgaacgctca cgcttggccg cgcatgcggt ggcggttttg taaggtttta
     4261 caggccgcat ggcagtatga aagtaatagc agtaaggtat tgagcctgtg tagcatccca
     4321 agcggcctgt aaactcaggc cgttttgtat tacaccctgt taatacaggt tcatagactt
     4381 gtcccctgct gccaccatac acatcggttt gaacaaggta aggcttgatt tgtgtctgta
     4441 tgtgtttcaa atgcgcactg catcacaaac accaacgctt atacttttcc aatacgcggc
     4501 aagtgtctga atgtacttgt cataggttta catgggttta caggccgctt acaggcttcc
     4561 acaggggcgt cagaagcggc tgtgttagaa tgatggcttt gatgaatcct caccctgacg
     4621 cgggtgaagc aagtatgggc gggtatggca ttgaaacaaa gcgtggttta ttttttggcc
     4681 ttgtgtgtgg tctttttcag caatttggct cacgccgcca tagaccccaa ctccatctta
     4741 cctgctgaag aagcctacaa aacccagctg tttgccaccg aagacggcgt ttctttgcaa
     4801 ttcaccatcg ccgatggcta ttacctctac aaagacaaat tggaattcaa cagcgacggc
     4861 ccaagcttgg gcaagcctga attcagccct gcccaatcca ccaccgatga atttttcggc
     4921 acccaagccg tgtatttcaa agtggcgcaa atcaacatcc cgtttagcaa agcacacaat
     4981 cccgcccagc cttaccgctt tcatttgaat taccaaggtt gcgccgaagt gggcatttgc
     5041 tatccgccca ccaaagttga atttgcggtg gacaaagtca gcttgattgc gccactagcc
     5101 agcggcaaaa tcaatgtgtt cagcaattca agcaccccca attttggcag cccatccaac
     5161 aaaaccgcaa cgacatcacc cacttccagc caaaccgctt ccaatagcag cacgggcttt
     5221 aacctcaacc aagatgcggt gtggcgcagc ttgcttacct tcttcatcgc aggcataggc
     5281 ttgagtttga ccgcgtgcat gtaccctttg ctgcccatcg tgtccagtat cgtcgttggt
     5341 aacaagcgca gcaaacgcat cgcgttttgg ctgtcattat tgtatgtaca aggcttggct
     5401 ttgacctata ccgtggtggg cgtcatcacc ggtttaaccg gttctttgct cacggtgtgg
     5461 ttgcaacagg cttgggtggt gttggcggca gcggctttgt tagtggtgtt ggctttggcg
     5521 atgtttgatg tgatacaaat ccagttgcca aatcgcatcc aatcgtattt ccaacaacaa
     5581 tccagccgca tgagcggcgg caagcttgcg tctgtgtttg gcatgggcat gttgtcagcc
     5641 ttgattgtgg ggccttgtgt cgcgccacca ttggcttttg ccttgggcta catcgcccaa
     5701 agcggcaatg cggtcttggg cggcgcggct ttgtatgcca tggcgatggg cacaggcttg
     5761 cccttgattt tggtggccac ttttggcgcg catgtgttgc ccaaagcagg cacatggatg
     5821 aacagcatca aagtggtgtt tggctgtctg cttttggcag cagcggtgta catggcgcgg
     5881 ccatttttgc cgtattggct caccatttcg ctgtatgccg ccatcttgtt tggactcggc
     5941 ctgtatttaa tcacccgcag cttcaaagtg gtgagcaaat ggaaatacct gccactgact
     6001 ttgggtgctg ctttgctcgc ggtttcggct tatttctcag gcgaaaccgt gcgcggcaac
     6061 accacgcccc tacaccaagc cttgagcttg caagcaccga gccaagcgca ttttggccgc
     6121 caatacaccg atgtacaggc tttacaggcc gctatgaatg aagccttggc gcaagaccca
     6181 agccgccccg taattttgga tttttatgcc gattggtgcg tgacctgcat ccaaatgcaa
     6241 atgaacactt tcaataccga cacggtgaaa agccaagtgg atgagcagcg cttcttcacc
     6301 atagacgtga ccgccaacac cgccgaacac caagccttgc tcaaagaata cggcttgttc
     6361 ggcccgccag gcatcttcaa aatcaaccac gatggcaaac gcagcgcagc ggtattgggc
     6421 tttgttggcg cggatgagtt tgtgaagtgg gtaaaagagc cattacctta agctaaactc
     6481 ctcaactcaa gcattttcaa ggtcataaac cattacttga cggatttttt taaccagttt
     6541 aatggtgatg cctgccgcca agaaaacaaa taacaacagt gccaccccat gcgtgtgctt
     6601 ggggggtaaa gggttggtct gccccacata ttggttcagt aatttaggat caaccgaata
     6661 caccatttct ctatgacgca ttccaccctt cattttggaa accaatactg aagactgtat
     6721 gtcgttcaca ctgtaaaaat gtaattgttc aacaaaagta ctggaaaaaa atgctttttc
     6781 acttccggga tagactctaa ataaatcggg gagggttttc cgccaaatgg attctggtgt
     6841 ggtgatgtcc ataaagacat attcaccccc ttcagtatga aacttcattc cacgaaaggt
     6901 cccatcccga tacctcaaga gtttcagagc cacatcttct tctaatgtgt attccaccac
     6961 ctgcttttga gccgaaaatt tttgcatggg ttgcacataa ccccaaaccg atgcccccaa
     7021 aaaaatccaa aaaccaagca ataccgtgta tagcatcatc tgcaccaata aatggacgct
     7081 tacataggct gaatcaactg ggttgaatgg cttgctttta aactttattg tacctttgtg
     7141 aggcttaggc aaagctactc gtttcaaggc gagcctagaa ttgaacatac gcagaaaatc
     7201 tgcaaaactc catgcaaata tgcccattaa aatggcccac aacaccagcc aattcaagtc
     7261 ttggtcatca tagcgccact gaaaatcatt caatatgccg cacacttgat aagcgagcaa
     7321 caacatcaac acatcacgaa tcaaataccc acattttctt tgatgactgc acagcttggg
     7381 ggtgaacaca tacaagacca cacgcataaa caccagtgcc ataaaacacc aaaatacgta
     7441 attctcatac ccaattccca ttatgactcc ccatgtgttc tccgtctttc ggcttttctt
     7501 attgtgttac aggccgcatt aagcggcctg tagtgtgttg ctgtgcatct actaaatcaa
     7561 tgcactagca tacatggtca tgggtggatt gtgtaattcc aacaatttta cgttatgccc
     7621 accaattact gttacggctg tacccacaga ccgtggcgct ccaaattggc tttttcatat
     7681 ggcgcgtctt cagcgggata gcgcaagcgc ttttcaccct tggctttggc caaag
//
LOCUS       DAOMVK010000046         7719 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_2477_length_7719_cov_2.588857, whole genome shotgun sequence.
ACCESSION   DAOMVK010000046 DAOMVK010000000
VERSION     DAOMVK010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 7719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7719
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2477_length_7719_cov_2.588857"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..623)
                     /locus_tag="PK856_02735"
     CDS             complement(132..623)
                     /locus_tag="PK856_02735"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRL01321.1"
                     /translation="MLNLRDFQMRDMPHLLQAIVDEKMLMQFAGPAYVFPLTAAQVAV
                     DLHADDVRVFSMVTAADEVVGHAQMQVLSDSVLLRRILIWDGSWRGQGWGQHMVRQLL
                     EVAFARPEVKQVRLNVFAWNTPAIRCYQRLGFVMSVESAKTVSVAGETWQSVGMVCVK
                     PVC"
     gene            complement(732..1856)
                     /locus_tag="PK856_02740"
     CDS             complement(732..1856)
                     /locus_tag="PK856_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003296525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRL01322.1"
                     /translation="MQTLWNIYRLGVKELWSLWRDPIMLVLIVFSFTFSVYSSSKAVS
                     ETLTRAPIAIVDEDQSQLSNKIAAAFYPPHFTEAAMVSFQDIDRGMDEGLYTFALIIP
                     HGLQADVVAGKAASVQLNVDATRMSQAFSGSGAIQQIVNAEVAEFSQRYRGNSSLPVE
                     LALRARFNPTLDNTWLSSIMQVVNNVTMLSIILTGAALIREREHGTVEHLLVMPVTVF
                     EIMTAKVWSMSVVVLLASFVSLHVVVRWALGVPITGSLSLFFMGAVLLLFATTSMGIF
                     MATVAKSMPQFGLLLMMVLLPLQMLSGGQTPRESMPDIVQNIMLAAPTTHFIKLAQAI
                     LFRGAGFEVVWPSFLSLFVIGSVFFYLALKRFKNTIHTMA"
     gene            complement(1860..>4375)
                     /gene="rbbA"
                     /locus_tag="PK856_02745"
     CDS             complement(1860..>4375)
                     /gene="rbbA"
                     /locus_tag="PK856_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HRL01323.1"
                     /translation="YHSLSVEENLQFFSRLFGHNAAERRRRIDDLTQSTGLFKFLDRP
                     AGKLSGGMKQKLSLCCALIHDPDLLILDEPTTGVDPLARAQFWDLIQRIRVDRPHMSV
                     MVATAYMDEAQTFDWLVMMDAGKVLATGTPEELLGAVACSDLESAYIQLLPAEKKQNH
                     QAIHIPPLQVDENEAYAIEAQDLTIRFGDFTAVDHVNFKIRRGEIFGFLGSNGCGKST
                     TMKILTGLLPATEGKAWLFGQEVNSGNIETRRKVGYMSQAFSLYTELTVLQNLVLHAR
                     LYHVPEGELDARVAAMMQRFELVEIQNALPDALPLGVRQRLSLAVALVHNPEILILDE
                     PTSGVDPVSRDNFWHYLIQLSRQDKVTIFISTHFMNEAERCDRMSMMHAGKVLDSDAP
                     AALVAKRQASSLEDAFIGYLLEAGAGTDVPANTVDTATASPQTHADTTPVALDTDTAQ
                     MPQPPLQAAYRPNRFSLQRLLSYSWCEALDLKRDRIRAIMALLGSIILMLVMGYGISM
                     DVEDLSYAVLDRDQSTLSSNYALSLSGSPYFIEKPMLHNEAEMDRRLQNGELALAIEI
                     PPNFAQKTGSGQPVAVAAWIDGAMPSRAETVQGYVQAIHQTWLAEQIKAKTGASLGSA
                     VTVETRYRYNPDVKSLPAMVPAVMPLLLLMLPAILTALSVVREKENGSIINLYVTPVS
                     KAEFLLGKQMPYIALGFVNYLLMTLLAVTLFGVPIKGSFFILSAAALLYVIIATGMGL
                     LASTLTKSQIAAMFFTLIGTITPAIQFSGMLNPVSSMQGFGRVFGEVYPATAMINISR
                     GVFNKALGFADLHHNLWLLALTIPVVLGMAIVLLKKQER"
     assembly_gap    4376..4385
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4386..4688)
                     /locus_tag="PK856_02750"
     CDS             complement(<4386..4688)
                     /locus_tag="PK856_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRL01324.1"
                     /translation="MEAQARGGQTDTVVANISAVSLRYGATLALDAIDLQLPAGKMVG
                     FIGPDGVGKSSLLSLIAGSRQLQEGSVWVLGGDMRAKKHRQRVCPNIAYMPQGLGKN"
     gene            complement(4688..5758)
                     /locus_tag="PK856_02755"
     CDS             complement(4688..5758)
                     /locus_tag="PK856_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HRL01325.1"
                     /translation="MNPAKIKTYIPYLLGAVVVAAVAYFVWQSQQEGDYAGFAKGNGR
                     IEATEIDLSAKIGGNVEDIFVKEGDFVKAGQVLAKIKAHALEAQLKEAKAQLEQANNA
                     VLTTQAQVQMGKSDKQAAIASVAQRNTELDAAKRRVARSEVLAKEGAASKQQLDDDRA
                     SVQAAVATLAAARAQVDTAEASIKAVEAQVVGAKSNVNAVQATIERIEAELSDTEIVA
                     PRDGRIQYLVAQKGETVASGGKIMNVVDLSDVFMTFFLPETVAGKVPVGTDVRLVLDA
                     LPTYAIPAKVSHVADTAQFTPKTVETESERQKLMFKVKAKIPPELLQQHLQLVKTGLP
                     GVAWVRLDAKQDWPTGITKKAE"
     gene            complement(5769..7169)
                     /locus_tag="PK856_02760"
     CDS             complement(5769..7169)
                     /locus_tag="PK856_02760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HRL01326.1"
                     /translation="MNKSVCGALLLSLMACQSPQKQHAIPQDTEQLVGAYTYAPAAQR
                     QVLQWDAYFQDPVLKDLLGQALAANKDLQLASLQILEAQALYGIQRADALPSINAGMD
                     AQRARSAADLRLPTYPAISQQYSASMGLQNWELDLWGRIASLNRAALQRFFASEANAL
                     AVRNSIMSQVSNQYLALAELDERIALATQSRVTFQESVRIFKRRYEVGSGAKTEYVQA
                     QTLLSNADTLLQQLQQQRDKHEHMLAVLVGGPVHKLTGRLDTQHFVSQAVLAGTPSDL
                     LLNRPEIIAAEHQLQAAHADIQAARAAFFPRIALTANVGTASNEFNQLFSSGNGIWSL
                     VPSISVPIFNAGRLRANVDLAQIRQSVAVVNYEKAIQTSFREVADALSDQHWLQQQLR
                     TQQQGLQAASERVRLARISYDYGVVTYLEVLDAERELLNAQQNWVQSKRQLLQAQTAL
                     YFALGGDAYLTSSKNQ"
BASE COUNT         1905 a         2192 c         1824 g         1788 t
ORIGIN      
        1 aagcatcgca acaacacttg ggcaaaatcg tgctcaacca tcctgtggca gcgcaatagc
       61 attcttctcc aatcaaacat aaaagcggcc tgtaaaccct aatatggttt acaggccgct
      121 tggccattgg attaacacac aggttttacg cacaccatgc ccacagactg ccatgtttca
      181 cctgccacag acactgtttt cgcggattct acgctcatca caaatcccaa gcgttggtag
      241 caacgaatgg ccggcgtgtt ccaagcaaac acattcaagc ggacttgctt tacttcaggt
      301 ctggcaaaag ccacctccaa caattgccgc accatgtgtt ggccccagcc ttgtccacgc
      361 caagacccat cccaaatcaa aatgcgccgc agcaacacac tgtctgacaa aacctgcatt
      421 tgcgcatgtc ccaccacctc atctgctgca gtgaccatgc tgaacacgcg cacatcatcg
      481 gcgtgtaaat ccactgccac ttgcgcagcc gttaagggaa acacatacgc agggcctgca
      541 aattgcatca gcattttttc atcgactatg gcttgcagca aatgcggcat gtctcgcatt
      601 tgaaaatctc tgaggtttaa catacacagc ccttgggcag acagtttgcg caggcgccaa
      661 tctaacatgg ctcttgggca cagcaccagc tcaaacccaa tcaatacccc atacatgcgg
      721 cctgtaaagg cttaagccat ggtgtgaatg gtgtttttaa agcgttttaa agccaagtaa
      781 aaaaacacgc tgccaataac aaacaaactc aaaaacgatg gccacaccac ctcaaaccct
      841 gcgccacgaa acaaaatcgc ttgcgccaat ttgatgaagt gggtagtggg tgcagccaac
      901 atgatgtttt gcacaatatc gggcatgctt tcgcgcggcg tttgaccgcc cgacagcatt
      961 tgcagcggca acaacaccat catcagcaac aagccaaact gcggcatgga ttttgccacc
     1021 gttgccataa aaatgcccat ggaagtggtg gcaaacaaca gcagcaccgc acccatgaaa
     1081 aacaagctta gcgaacccgt gatgggcaca cccaaagccc agcgcaccac cacatgcaaa
     1141 gacacaaacg atgccagcaa caccaccaca ctcatggacc agactttggc cgtcatgatt
     1201 tcaaacaccg tcacaggcat caccagcaaa tgctctaccg tgccgtgttc acgctcgcgt
     1261 atcaaagcgg cacccgtcaa aatgatgctc agcatggtga cattgttcac cacttgcatg
     1321 atgctgctga gccaagtgtt gtccaaagtg gggttaaaac gcgcccgcaa agccagctcc
     1381 acaggcaagc tgctgttgcc gcggtagcgt tggctgaatt cggccacctc ggcattgacg
     1441 atttgctgta tcgcaccgct gcccgaaaaa gcctgactca tgcgcgtggc atccacattc
     1501 aattgcacac tggccgcctt acccgccacc acatcggcct gtaaaccatg cggtatgatt
     1561 aaggcaaacg tgtacaagcc ctcatccatg ccgcggtcta tgtcttgaaa actgaccatc
     1621 gccgcttcgg taaaatgcgg tggataaaaa gccgcagcga ttttattcga caattgcgat
     1681 tggtcttcat ccacgatggc gatgggcgca cgggtcaggg tttccgaaac cgctttggag
     1741 ctggaataca ccgaaaaagt gaaggaaaac acaatcaaca ccaacatgat ggggtcgcgc
     1801 cacaaactcc acaattcttt cacgcccaag cggtaaatgt tccacagagt ttgcatcaat
     1861 caacgctcct gctttttcaa taacacaatg gccatgccca ataccacagg gatggtcaag
     1921 gccaacagcc acagattgtg gtgcaaatcg gcaaatccca aggctttgtt aaacacaccg
     1981 cggctgatgt taatcatggc ggtggcagga taaacctcgc caaacacccg cccaaagcct
     2041 tgcatggacg acacgggatt gagcatgccc gagaactgta tcgcgggcgt gatggtgcca
     2101 attaaggtga aaaacatcgc ggcaatttgg cttttggtta aggtagacgc caacaagccc
     2161 atgcccgtgg cgatgatgac gtacaacaaa gcggccgcag acaagatgaa aaaactgcct
     2221 ttaatcggca cgccaaacag cgtcaccgcc aacaaagtca tcaacaaata attgacaaaa
     2281 cccaaggcga tgtaaggcat ttgcttgccg agtaaaaatt ccgccttgct cactggcgtg
     2341 acatacagat tgatgatgga gccattttct ttttctcgca ccaccgacaa ggcggttaag
     2401 atggcgggca acatcaacag caacaaaggc atcaccgcag gcaccatcgc aggcaggctt
     2461 ttcacatcgg gattgtaacg atagcgggtt tccaccgtta ccgcgctgcc caagcttgcg
     2521 cctgttttgg ctttgatttg ttctgccagc caagtttggt gtatggcttg cacatagcct
     2581 tgcacggttt ctgcccgcga aggcatggca ccatctatcc acgccgccac tgccacaggc
     2641 tggcctgagc cggttttttg ggcaaaattg ggcggtatct ctattgccaa agccagctcg
     2701 ccattttgca aacgcctgtc catctcggcc tcattgtgca acatgggttt ttcaataaag
     2761 taaggtgaac cagacaggct caaggcataa ttggaactga gggtggattg gtcacggtcg
     2821 agcaccgcat acgacaagtc ttccacatcc atgctgatgc catagcccat taccagcatc
     2881 aatataatcg agcccaacaa agccatgatg gcgcggatgc ggtcgcgctt caaatccaag
     2941 gcctcgcacc aactgtagct gagcaaacgc tgcaaactga agcgattggg gcgataagcg
     3001 gcctgtaaag gtggctgagg catttgtgcc gtgtctgtat ccaatgccac gggtgtcgtg
     3061 tctgcatggg tttgcggcga tgctgtggca gtatcaacag tattggcagg cacatccgtg
     3121 cccgcgcctg cttccaacaa atagccgatg aacgcgtctt ccaaactgct ggcctgacgt
     3181 ttggccacca aagccgcagg ggcatcgctg tccaacacct tgcctgcgtg catcatggac
     3241 atgcggtcgc aacgctctgc ctcgttcatg aaatgggtag aaataaaaat cgtcaccttg
     3301 tcttggcgcg acaattgaat caaataatgc caaaaattgt cgcgtgacac agggtctacc
     3361 cccgaagtgg gctcatccaa aatcaagatt tcagggttgt gtaccaaagc caccgccaaa
     3421 gacaagcgct gtcttacccc caaaggcaag gcatcgggca aggcattttg tatctcaacc
     3481 aattcaaacc gctgcatcat cgccgccacg cgcgcatcca gctcgccctc aggcacatgg
     3541 tacaaacgcg catgcaaaac caagttttgc aacacggtca attctgtgta cagcgaaaac
     3601 gcctgcgaca tatagcccac cttgcggcgg gtttcaatat tgccactgtt gacctcttgc
     3661 ccaaacagcc aagccttgcc ttcagtggca ggcagcaagc ccgtcaggat tttcatggtg
     3721 gtggatttac cgcagccatt cgagcccaaa aagccaaaaa tctccccacg gcgtatctta
     3781 aaattcacat ggtctacggc cgtaaaatcg ccaaagcgta tggtcaagtc ttgcgcctcg
     3841 atggcatagg cttcgttttc atccacttgc aagggcggaa tgtgtatggc ttggtggttt
     3901 tgcttttttt cagctggcaa caattggatg taagccgact ccaaatcgct gcaagccaca
     3961 gcaccaagca attcttctgg cgtgcctgtg gccaatacct tgcccgcatc catcatcacc
     4021 agccagtcaa atgtctgcgc ttcgtccatg taagccgtgg ccaccatcac actcatgtgc
     4081 ggtctgtcca cgcgtatgcg ctgtatcaaa tcccaaaact gcgcgcgggc caaagggtct
     4141 acgccggtgg tgggctcatc caaaatcagc aaatcggggt cgtgtatcaa ggcgcagcac
     4201 aagctcagct tttgcttcat gccacctgac agcttgcctg caggtcggtc taaaaactta
     4261 aataagccag tgctttgggt caaatcatcg atgcgacggc ggcgctcagc cgcattgtgg
     4321 ccaaacaagc gtgaaaaaaa ttgcaggttt tcctccaccg acaggctgtg gtacannnnn
     4381 nnnnngttct tgcccagacc ttgcggcata taggcaatgt taggacacac gcgttggcgg
     4441 tgtttcttgg cgcgcatgtc gccgcccaac acccacaccg agccttcttg taattggcgc
     4501 gaacctgcaa tcaaagacaa taagctcgac ttgcccacac catcagggcc aatgaagccg
     4561 accattttgc ctgcaggcag ctgcaaatcg atggcgtcca aagccaaagt tgcaccatag
     4621 cgcaagctga ccgcgctgat gttggccacc acggtgtctg tttgaccccc tcttgcttgt
     4681 gcctccatca ctcagccttt ttggtgatgc ctgtcggcca atcttgcttg gcatccaagc
     4741 gcacccaagc cacgcctggc aggccggttt tcaccaattg caaatgctgt tgcaacaatt
     4801 caggtgggat tttggccttg accttaaaca tcagcttttg gcgttcactt tcggtttcca
     4861 cggttttggg ggtgaattgt gcggtgtcgg ccacatgcga tactttggcg ggaatggcat
     4921 acgtgggtaa ggcatccaaa accaaacgca catcagtgcc cacaggcact ttgccagcaa
     4981 cggtttcagg caggaaaaac gtcatgaaca catcagacaa atccaccaca ttcatgattt
     5041 tgccacccga agccacggtt tcgccttttt gcgccaccaa atactggatg cggccatcgc
     5101 gaggcgcgac aatttcagtg tccgacagct cggcctcaat gcgctctatc gtggcttgga
     5161 ccgcattcac attcgatttc gcccccacca cttgcgcttc aaccgctttt atcgatgcct
     5221 ccgccgtgtc cacttgcgca cgtgctgctg ctaaagtcgc cacggcggcc tgtacgctgg
     5281 cacggtcgtc gtccaattgc tgtttggaag ccgcgccctc tttggccaac acttcagaac
     5341 gggccacgcg gcgtttggcc gcatccaatt cggtattgcg ctgcgccacg ctggcgatgg
     5401 cggcttgttt gtcgctcttg cccatttgta cttgcgcttg cgtggtcaat acggcgttat
     5461 tggcttgctc caattgcgct ttggcttcct tcaattgggc ttctaaggca tgggctttga
     5521 ttttggccaa cacttgccct gctttgacaa agtcgccttc tttcacaaag atgtcttcaa
     5581 cattgccgcc aattttggca gacaaatcga tttccgtggc ttctatgcgg ccattgcctt
     5641 tggcaaagcc tgcataatcg ccctcttgct ggctttgcca cacaaaatat gccacggcgg
     5701 caaccaccac cgcgccgagc agatagggaa tgtatgtttt gatttttgct ggattcatga
     5761 tgcaagacct attgattctt cgaggatgtt aagtaagcgt cgccacctaa ggcgaaatac
     5821 aaagccgttt gcgcttgcaa gagttggcgt ttgctttgca cccagttttg ttgggcgttc
     5881 agcaattcgc gctcagcatc caaaacttcc aaatacgtca ccacgccata gtcataactg
     5941 attctggcca aacgcacccg ctcagatgcg gcctgtaagc cttgttgttg tgtgcgcaat
     6001 tgctgttgca gccaatgctg atcactcaag gcatcggcca cctcgcgaaa cgaggtttgg
     6061 atggcttttt cataattcac caccgccacg ctttggcgga tttgtgccaa atccacattg
     6121 gcacgcaaac gccctgcgtt gaaaatcggc acactgatgc tgggcaccaa gctccagatg
     6181 ccattgccac tgctgaacaa ttgattgaat tcgttgctgg ctgtgcccac gttggcggtt
     6241 aacgcgatgc ggggaaaaaa tgcggcgcga gcggcctgaa tgtccgcatg tgccgcttgc
     6301 aattggtgtt ctgcggcaat gatttcaggg cgattgagca gcaaatccga tggcgtaccc
     6361 gcgagcacgg cttggctgac aaagtgttgc gtgtccaagc ggcctgtaag cttgtgcaca
     6421 ggccctccca ccaataccgc aagcatgtgc tcatgcttgt cacgctgttg ctgtaattgc
     6481 tgcaacaaag tatcggcgtt gctgagtaag gtttgcgcct gcacatactc ggttttcgcc
     6541 cccgaaccca cttcataacg gcgcttgaaa atgcgcaccg attcttgaaa cgtgacgcgt
     6601 gattgtgtgg ccaaggcgat gcgttcatcc aattctgcca aggccaaata ttgattgctc
     6661 acttggctca tgatgctgtt gcgcacagcc aaggcattgg cttcgctggc aaaaaagcgt
     6721 tgcaaagccg cgcggttcag gctggcaatg cgtccccaca aatccaattc ccaattttgc
     6781 aaacccatac tggcgctgta ttgctgagaa atggcaggat acgtgggcaa gcgcaaatca
     6841 gcggcgctgc gagcgcgctg cgcatccatg ccagcattga tgctgggcaa ggcatcggcg
     6901 cgctgtatcc catacagcgc ttgtgcctca agtatttgca aactggccaa ctgcaagtct
     6961 ttgtttgccg ccaacgcttg accaagtaag tcttttaaga ctgggtcttg aaaataggca
     7021 tcccattgca aaacctgtct ttgcgccgcg ggtgcatagg tgtatgcccc gaccaattgt
     7081 tcggtgtctt gtggtattgc gtgttgcttc tgcgggcttt gacaagccat caggcttagc
     7141 aacaaggccc cacaaacact tttattcatc aaaccgcctc tcacctgtcc tcctgtctgt
     7201 caaccatgcg gcctgtaaac aaaaacaaca ccgctgtgag cttgattaca gcagtttagt
     7261 tggctaattt ttcagggtca aaatcgcgaa tagcctctat tttatgttaa tcattacaga
     7321 ttattttgca tccatcccca tatacgacat catttgcgca acaatatacc aatgcctcaa
     7381 catctgttac ccacccctga aacattgttc atctacaagc gcatctctat gctgcatgaa
     7441 taaatctttt gccgcgcatg ctttgatttg catcagggtt ttgggattta ttcttgcagc
     7501 tcagtgtggt atttaacata catgcaagta tgtattgata tgcgccaact gcgcccacac
     7561 aacccgcaag tcgccgcaag ccgccatacc aatcccaaac tcatgtaccg cagtagcaca
     7621 aatcacgttt cagcagtttg cgcagccatg ccgaaagtaa gcatagatta attcccatct
     7681 acagcgccaa agatgccgct ataatcaacg cggctgatg
//
LOCUS       DAOMVK010000047         7667 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_2508_length_7667_cov_3.079480, whole genome shotgun sequence.
ACCESSION   DAOMVK010000047 DAOMVK010000000
VERSION     DAOMVK010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 7667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7667
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2508_length_7667_cov_3.079480"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(205..588)
                     /locus_tag="PK856_02765"
     CDS             complement(205..588)
                     /locus_tag="PK856_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01327.1"
                     /translation="MKPILQFSQVLHTLQQERANCIAALRHQNDDATALAYKQQLDAA
                     IACLQWCERYQVSGNSPVQVLPFPRNAFGEYVLLEADESGDYLPEPVRDSHQHAIAPD
                     AGDLIVRLSHVLNLQNNAEKPTSGL"
     gene            679..1578
                     /locus_tag="PK856_02770"
     CDS             679..1578
                     /locus_tag="PK856_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01328.1"
                     /translation="MKTWDTLFWQCEDLAALQAHIFSADELWVRAQLLQQFAALKTYH
                     NAEEWNRLVRVCEGLRMVGWGEAEPVEAIAQQWINGAWYTRLQNSAFVCVDGGQEQEW
                     RVQQGVQVLALQPGSIGGLAKLHSQRNPLPKSPIRWQYSGNYDRELQALIDSFERLKQ
                     LLIAHSRPETYGHDFAYVAWHWHFSHLNDEYIPAEYYHHEADVPEAQRSSQHPAYWIR
                     PEYKVGRMALAKYETRLSITRHFNTAFSHAGLAAQQAQIAADLCFGAAQVRKKLQAKK
                     SAYDCDAWEHDLHQILNMWQQSQ"
     gene            1624..2274
                     /locus_tag="PK856_02775"
     CDS             1624..2274
                     /locus_tag="PK856_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01329.1"
                     /translation="MLKMPYTRPVLLLLLCASAFSAHAAAPSDKQIVNWLTNKDKIVA
                     EKVNSNTVTINQTEAITLQSGEQAYLSAVEYADSGRNFSNGYILTRPQLKQSQHLESF
                     AGQTSSIEVLSRKRKATLLELESSGSGQGTMASAVSLVTFNGWKPVVHYKSESYDDFG
                     YASETRGCTSIEAKIKFNDGNPAKVTETIKTLHQKNCDNPKTAKTKTETKVKTIAW"
     gene            complement(2361..3713)
                     /locus_tag="PK856_02780"
     CDS             complement(2361..3713)
                     /locus_tag="PK856_02780"
                     /inference="COORDINATES: protein motif:HMM:NF025089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRL01330.1"
                     /translation="MYFHQEDAAVSQLLLDTLKPLVNTTSNIVIIGNTSLDFITRVQD
                     LCPKAHVHAFQAGQNWWLGDFHGVLQQFLQDLPEQSVDCLLLKDFMQLQDHSIAWLQA
                     ARRALNPQTTVIANIKNSQHHQYIQQLLSQNSHNYTTVKFGNVAKLSLMEGIKVFLDA
                     GMLPYLNGSLPQVADDAFITSLQAALNLLGMQERVSKTRLNQLQYIFTAKMLPERLQN
                     PVAEPCSVLCATNNALQYQNNLAVSPDLLQEDTPHQLLALSNAPNIAAAFAEGIKHCE
                     HTWIVLVHQDVYLPQGWLADIQHAWHAAEQKLGHAIGVAGVAGMVQNAAGNFDFAGCA
                     MDRIYTFHHSADQLPAQAHSLDELVLVYRRDRFIPELNPELGFHYYATDSVFKAEAAG
                     LVGAILPVPCLHNTSRYDTTDNDADVRQKSQIFRQNWQHRLPIRTTIAHLTDEGCQYY
                     "
     gene            complement(3765..4523)
                     /locus_tag="PK856_02785"
     CDS             complement(3765..4523)
                     /locus_tag="PK856_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRL01331.1"
                     /translation="MFDWLWPESISHATTWFLIVFSCLTSAITASLGAGGGVLLLATM
                     AMVLPPAVIIPVHGIVQLGSNFNRAIMTLKDLDKRLIGIFALGSALGAWLASLFLVQL
                     PIQTTQLTIAALILFLCWGPALPNVALGKLGTFVAATITTFISMFAGATGPLVGAFVK
                     QQQHGDRARTVANFSAAMTVQHLPKTIVFGATGFVFQEWLGLIVLMVISGAIGTWVGL
                     KIAHKISNRNFGLIFNLLLTALALRLVWQAWQSS"
     gene            complement(4548..4904)
                     /locus_tag="PK856_02790"
     CDS             complement(4548..4904)
                     /locus_tag="PK856_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01332.1"
                     /translation="MMPTTHPLSAYIRASMHLQDLPNVVSQQRQEAQQQDGAYTILTQ
                     TTKYIFDNGVHMVAEVEQEWSQHDDIQGDVCPPCDISYAITEAAGFDIQPATKSFKNT
                     CQLHFWVQAHHLHPPQ"
     gene            complement(4945..5865)
                     /locus_tag="PK856_02795"
     CDS             complement(4945..5865)
                     /locus_tag="PK856_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HRL01333.1"
                     /translation="MKLQQLRYTLEVYKHNLNVSEAAEALFTSQPGISKQIRLLEDEL
                     GIQIFIRNGKRVVSVSEPGKQVLQVAERMLRDAQNIKRIGAEFSEQDAGSLTIATTHT
                     QARYVLPKIIAEFVARFPKVQLSIKQGPPNADFAIATEEIDDYPELSKLPCYQWNRSV
                     VVPDGHPLLDLDRPLRIADIAQYPLVTYEFAFTGQSQIARGFIRAGSPPPHVALSAVD
                     TDVIKTYVRLGLGVGLIASMAFLPDEDKGLHLINAEHLFEPSTTQIALRQDSYLRGFA
                     YDFIQLFEPSLSRERISQILFAPINEDFSI"
     gene            6322..6981
                     /locus_tag="PK856_02800"
     CDS             6322..6981
                     /locus_tag="PK856_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRL01334.1"
                     /translation="MTKQLKLGALIAALVASTGALAHEKPGYTISEQSREVVRNSYGE
                     CWENTYLDKAANGLAECGDAPAKQVITEEVNETVRLQSEFLFGFDKYNLRPEAITTLD
                     SLAARLGQTNVQAVRVEGNTDFMGSEQYNQALSERRANTVANYLVSRGVAASKISAVG
                     LGESQQQLQAQCEAEVAKLGKKVSKAKKRSALIACIEPDRRVDVQIRTLVTREVQKTA
                     N"
     gene            complement(7040..>7667)
                     /locus_tag="PK856_02805"
     CDS             complement(7040..>7667)
                     /locus_tag="PK856_02805"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003787791.1"
                     /note="AckA utilizes acetate and can acetylate CheY which
                     increases signal strength during flagellar rotation;
                     utilizes magnesium and ATP; also involved in conversion of
                     acetate to aceyl-CoA; also known to act on propionate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HRL01335.1"
                     /translation="WRHRHAEYSVCMVVAHLGNGASVTAIKNGISVDTSMGLTPLEGL
                     IMGTRSGDIDPSIFEFLFDNKHMSIRQINSMLNKDSGLLGISELSNDCRTLEEHAALG
                     HDGAILALNMFAYRLAKYIASMTVATGRLDALVFTGGIGENSASMRKRVMQHLGFLGV
                     HVDDTLNQTMVGGQAGHINLVTDTVASLVIPTNEELMIAKDTYALTIK"
BASE COUNT         1969 a         1724 c         2024 g         1950 t
ORIGIN      
        1 ccgcctgcaa tggttggtgc acgattgcca tcaccaaaac caagacttgc ctgaaagcgt
       61 gaatgcaagt gtggtgcaag agcgtttgta cgcgctcaat tggttaacag gctttgatgt
      121 ggccgagtgg gacgacattg atacgcctgc ttaaatatgt gatttaggaa tgattaagcg
      181 gcctgtaaat actacttaaa acatttatag gccgcttgtg ggcttctcag cattattttg
      241 cagattcaac acatgactta agcgcacaat caaatcgcct gcatcaggtg caatggcgtg
      301 ttgatgagag tcgcgaacag gctcaggcaa ataatcgccg ctttcatcgg cttcgagcaa
      361 cacatattct ccaaacgcat tgcgcggaaa cggcaacact tgcacagggc tgttgccaga
      421 cacttggtag cgttcgcacc actgcaagca ggcgatggcg gcatcgagtt gttgtttgta
      481 ggccaaggcg gttgcgtcat cgttttggtg gcgcaaggcg gcgatgcagt tggcacgttc
      541 ttgctgtaag gtgtgcaaga cttgagaaaa ttgtaagatg ggtttcatga ctggctcagc
      601 aggtttacag gccgcttgat tatcaggtgc acgcgcacat ttgaacagct tcttttggat
      661 gacatcgtgg acacatacat gaagacgtgg gatacattgt tttggcagtg tgaggattta
      721 gccgccttgc aagcgcacat cttcagtgcc gatgagctgt gggtgcgggc acaattgttg
      781 caacagtttg ctgccttaaa aacctatcac aatgccgaag aatggaaccg attggtgcgg
      841 gtgtgtgagg gtttgcgcat ggtgggctgg ggcgaggccg agcctgtgga ggcgattgcg
      901 cagcaatgga taaacggcgc ttggtatacg cgtttgcaaa acagtgcgtt tgtctgtgtg
      961 gacggtgggc aagagcaaga gtggcgtgtg cagcaaggtg tgcaagtttt ggcgctgcag
     1021 ccaggcagca taggcggctt ggccaagttg cactcccaac gcaatccctt gcccaaatcg
     1081 ccgatacgct ggcagtattc aggcaattat gaccgcgagt tgcaagcttt aattgacagt
     1141 tttgagcgct taaaacaatt gctcattgcc cacagccggc ctgaaaccta tggccatgat
     1201 tttgcgtatg tggcttggca ttggcatttc agccatttga atgatgagta cattcctgcc
     1261 gagtattacc accacgaggc cgatgtgcca gaggcgcagc gcagcagcca acaccctgct
     1321 tattggatac ggcctgaata caaagtgggg cgtatggcct tggccaaata cgaaacccga
     1381 cttagcatca cccgccattt taataccgcg ttttcacatg cgggtttggc tgcgcagcaa
     1441 gcgcaaatcg cggctgattt gtgttttggt gcggcgcaag tgcgcaagaa attgcaggcc
     1501 aaaaagagtg cttatgattg tgatgcttgg gaacacgatt tacaccaaat tttaaacatg
     1561 tggcagcaat ctcaataagc tgcgtgccat cttattcata tttcaaaaac agaaaggtcg
     1621 accatgctaa aaatgccata cacccgtcct gtattgctct tgttgctgtg tgccagcgca
     1681 ttcagcgccc atgctgctgc gcccagtgac aagcaaatcg tcaattggct caccaacaaa
     1741 gacaaaatcg tggccgaaaa agtgaacagc aacactgtga ccattaacca aaccgaagcc
     1801 atcaccttgc aatcgggtga gcaagcatat ttgtcggcag tggaatatgc cgattcgggc
     1861 cgcaattttt ccaatggcta tattttgaca cgcccgcaat tgaagcaatc acaacacttg
     1921 gaaagtttcg cagggcaaac gtccagcatt gaggtgttga gccgcaagcg caaggcgact
     1981 ttattggagc tggaaagctc gggctcggga caaggcacga tggcctcagc cgtgagcttg
     2041 gtcactttca acggctggaa gcctgtggtg cattataaat cggagtctta cgatgatttt
     2101 ggctacgcct cagaaacccg tggctgtacc tcgatagagg ccaaaatcaa attcaatgac
     2161 ggcaaccctg ccaaagtcac cgaaaccatc aaaacactgc accaaaaaaa ctgtgacaat
     2221 cccaaaaccg ccaaaaccaa aaccgaaacc aaggtgaaaa ccattgcttg gtagttgaat
     2281 tggtaaggat ggaaatcaat acacaagcgg cctgtaacat ttacaggccg cttgtttgct
     2341 gtttgagcga gtggatgggt tcaataatat tgacagcctt catcggtgag gtgggcgatg
     2401 gtggtgcgta tgggcaaacg gtgttgccag ttttggcgaa agatttggct tttttgcctc
     2461 acatcggcat cgttatcagt ggtgtcgtaa cgcgaagtgt tgtgcagaca aggtactggc
     2521 aagatggcac caaccaaacc cgcagcttcg gctttgaaga ctgagtcggt ggcgtaatag
     2581 tgaaaaccca gctcaggatt gagctcagga atgaagcggt cgcgtcggta taccaaaacc
     2641 aattcgtcta aagagtgtgc ttgcgctggt aattggtcgg cagaatggtg gaaggtgtaa
     2701 attcggtcca tggcgcagcc cgcaaagtca aaattgccag ctgcattttg caccatacct
     2761 gccacaccag ccacacctat ggcatggccc aatttttgtt ctgctgcgtg ccaagcatgc
     2821 tgaatatccg ccagccaacc ttgtggcaaa tacacatctt ggtgtaccaa gacgatccat
     2881 gtgtgttcgc aatgtttgat gccttctgca aaagccgcgg cgatgttggg ggcattggag
     2941 agggcaagca attgatgtgg ggtgtcttcc tgtaacaaat caggcgaaac cgccaaattg
     3001 ttttggtatt gcaaggcatt gttggtggcg cacaacacac tgcacggctc ggcgactggg
     3061 ttttgcaaac gttcgggcaa cattttggcg gtgaagatgt attgcaattg gtttaagcgg
     3121 gtttttgaga cgcgctcttg catgcctaat aggtttaatg cggcttgtaa gctggtgatg
     3181 aaggcatcat cggcgacttg aggtaaagaa ccgttcaaat aaggtaacat gcctgcgtct
     3241 aaaaagactt tgatgccttc catcagcgat aacttggcca cattgccgaa ttttactgtg
     3301 gtgtaattgt gactgttttg gctgagcaat tgttgaatgt attggtggtg ttggctgttt
     3361 ttgatgttgg cgatgacggt cgtttgtggg tttaaagcac ggcgagcggc ctgtaaccaa
     3421 gcaatgctgt ggtcttgcag ctgcataaag tcttttaaca acagacagtc gaccgattgc
     3481 tctggcaaat cttgcaagaa ttgctgtaac acgccatgaa aatcacccaa ccaccagttt
     3541 tgtcctgctt gaaaggcatg aacatgtgcc ttggggcaca agtcttgtac acgagtgatg
     3601 aaatccaagc tggtgttgcc gataatgaca atgtttgatg tggtattgac cagcggcttg
     3661 agcgtgtcca gcagcagttg gcttacggca gcgtcttctt ggtggaagta catgcgttaa
     3721 tctttcagtt gggcagggtg tcagtgatgc atcttatgtg aagctcagga cgattgccat
     3781 gcttgccaca ccaaacgcag tgccagcgcg gttaatagta ggttaaatat caggccaaaa
     3841 ttgcggttgc tgattttgtg ggcaattttc aggcctaccc aagtgccgat ggccccgctg
     3901 atgaccatta acactatcaa gcccaaccat tcttgaaaca caaaacccgt ggcaccaaat
     3961 acgatggttt tgggcaggtg ttgcacggtc atggcggcgg aaaaattggc gacggtgcga
     4021 gcacggtcgc catgttgctg ttgtttcaca aaagcaccca cgagcgggcc tgttgcccct
     4081 gcaaacatgc tgatgaaggt ggtgatggta gccgccacaa aagtacccag ttttcccaag
     4141 gccacattgg gcaaggctgg gccccaacac aaaaacaaaa tcagcgcggc gatggtcaat
     4201 tgggtggttt gtatgggcag ttgcaccaaa aacaaactgg ccaaccaagc acccaaggca
     4261 gaacccaagg caaaaatgcc tatcaagcgt ttgtccaagt ctttgagggt catgatggca
     4321 cggttgaagt ttgagcccaa ttgcacaatg ccatgcacgg gaatgatgac agcaggcggt
     4381 aacaccatgg ccatggtggc caacagcaac acaccgcccc ctgcgcccaa gctggccgtg
     4441 atggcggagg tcaggcatga gaagacaatg agaaaccagg tggtggcatg ggaaatggat
     4501 tctggccaca gccaatcaaa catctaatac tcctttggag tgtggactta ttggggtgga
     4561 tgtaaatggt gggcttgcac ccaaaaatgc agttggcacg tattcttaaa tgatttggtg
     4621 gcgggctgta tgtcaaagcc agccgcttcg gtgatggcat agctgatgtc gcagggaggg
     4681 cacacatcac cttgtatgtc atcgtgttgt gaccattctt gttcgacttc ggccaccatg
     4741 tggacaccat tgtcaaaaat gtatttggtg gtttgggtga ggatggtgta ggcaccgtct
     4801 tgttgttgcg cttcttgacg ctgctggctc accacattgg gcaagtcttg taagtgcatg
     4861 ctggcacgga tgtaagcact taatgggtgg gtggtgggca tcatgtgttg taagcggcct
     4921 gtaaaccgtt acaggccgta ttccttatat gctgaagtct tcattaatgg gtgcaaacaa
     4981 aatctgactg atgcgctcac ggctcaacga aggctcgaac aattggatga agtcataggc
     5041 gaaaccacgc aaatagctgt cttggcgcag cgcgatttgg gtggtagaag gctcaaacaa
     5101 atgttccgca ttgattaaat gcaagccctt gtcctcatca ggtaagaagg ccatggaggc
     5161 aatcaagccc acgcccaagc ccaaacgcac ataggtttta atcacgtcgg tgtctaccgc
     5221 agataaggcc acatgcggag gtggcgagcc tgcgcgaata aagccacggg cgatttgcga
     5281 ttgaccagtg aaggcaaatt cgtaagtcac caatgggtat tgggcaatgt cggcgatgcg
     5341 cagtgggcgg tccaaatcca ataaagggtg gccatcaggc acgaccacac tgcggttcca
     5401 ttggtagcac ggcagtttgg acaattctgg atagtcatcg atttcttcgg tggcaatggc
     5461 aaaatcggca ttgggcggcc cttgtttaat cgataactgc actttgggaa aacgtgccac
     5521 aaactccgca atgattttgg gcaagacata acgcgcttgg gtatgggtgg tggcaatggt
     5581 gagtgagcct gcatcttgct cggaaaactc agcgcctatg cgcttgatgt tttgggcatc
     5641 acgcaacatc cgctctgcca cttgcaaaac ttgtttacca ggctcggaca ccgataccac
     5701 gcgtttgcca ttgcggatga agatttggat gcccaactca tcttctaata agcgaatttg
     5761 tttggaaatg cctggttgcg atgtaaacaa agcctcagca gcttcagaca cattcaaatt
     5821 gtgtttgtaa acttctaagg tatagcgcag ttgttgtaat ttcatatgct tagactttca
     5881 gtgcactgca ccaaaacagt taaatcacag atttgtggcg tatttgccgc atggatacat
     5941 gggtggaaac atgcagataa tctgacttta tgccaaacca ttttaacatt gtttgcctca
     6001 ctctcttggt atcggttgag ttgaaggcga gagtttgtcg gttaagtcgt ggaactgttg
     6061 ttgataacct acaaagcaag gaatatgttt ggcttaatgt tgtattaaag tgacaaaaga
     6121 ggtatttttg accctatgtt ctgtatgggt agttttgctg gcacttggga ttatgaagat
     6181 tgtattgaca gttttcagcc ttattcttat agtgcttact tgacatacct tagtgctccg
     6241 ccttgatggc gagtcagatt tttgtaatcg cttatgcact cccaagtgtt gcataaatga
     6301 aattttagat gagggaaaac catgaccaaa cagcttaaat tgggtgcatt gattgctgca
     6361 cttgttgctt ctaccggtgc attggcacat gaaaagcctg gctacaccat cagcgaacaa
     6421 tcacgtgaag tggttcgcaa cagctacggc gaatgctggg aaaacactta cttagacaaa
     6481 gcggcaaacg gtttggctga gtgtggtgac gctcctgcta aacaagtgat cactgaagaa
     6541 gtaaacgaaa ctgttcgttt gcaatctgaa ttcttgttcg gttttgacaa atacaacttg
     6601 cgtcctgaag ccatcactac tttggattct ttggctgctc gtttgggtca aaccaacgta
     6661 caagctgtac gtgttgaagg taacaccgac ttcatgggtt ctgaacaata caaccaagca
     6721 ttgtctgagc gccgtgctaa cactgttgct aactacttgg ttagccgtgg tgttgcagcc
     6781 agcaaaatct ctgcagttgg tttaggtgaa agccaacaac aattgcaagc tcagtgtgaa
     6841 gctgaagttg ctaaattggg caaaaaagtg tctaaagcga aaaaacgctc tgctttgatc
     6901 gcttgtatcg aacctgaccg tcgcgttgac gttcaaatcc gtactttggt aactcgcgaa
     6961 gttcaaaaaa ctgcgaacta atccaaacgg attaaaaaac ccgctgcttg cagcgggttt
     7021 tttgtttgaa cagtgagtat tatttgatgg ttaatgcgta agtgtctttg gcaatcatca
     7081 actcttcatt ggttggaatc accaagcttg caacggtatc ggtaacgaga ttgatgtggc
     7141 ctgcttgccc acctaccatg gtttgattca aggtgtcatc cacatgcact cctaaaaagc
     7201 ccaagtgttg catcacgcgt ttgcgcatgc tggctgagtt ttctccaatg ccgcctgtaa
     7261 acaccaaggc atccaagcgg cctgtagcca ctgtcatgga ggcgatgtac ttggccaagc
     7321 ggtaggcaaa catgttcaag gccaaaattg caccgtcatg acccaaggct gcgtgttctt
     7381 ccaaggtacg gcaatcgtta gacaattcag aaataccaag taagccactg tctttgttca
     7441 gcatgctgtt gatttggcgg atgctcatgt gtttgttgtc aaacaaaaat tcaaaaatcg
     7501 atgggtctat gtcaccactg cgggtgccca tgatcaggcc ttctaagggg gttaagccca
     7561 tactggtgtc gacagagatg ccatttttaa tcgccgttac cgatgcgcca ttccctaagt
     7621 gggcaaccac catgcaaacg ctatattcgg catgtcgatg gcgccat
//
LOCUS       DAOMVK010000048         7654 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_2518_length_7654_cov_2.941966, whole genome shotgun sequence.
ACCESSION   DAOMVK010000048 DAOMVK010000000
VERSION     DAOMVK010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 7654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7654
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2518_length_7654_cov_2.941966"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PK856_02810"
     CDS             complement(<1..320)
                     /locus_tag="PK856_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HRL01336.1"
                     /translation="MNKYLAEFFGTFWLVFGGCGSAVLAAAYPELGIGFAGVALAFGL
                     TVLTMAYAVGHISGGHFNPAVSVGLFVGGRFDGKDLLPYIVSQVLGAIAAAGVLYIVA
                     SGKAG"
     gene            complement(480..1322)
                     /locus_tag="PK856_02815"
     CDS             complement(480..1322)
                     /locus_tag="PK856_02815"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HRL01337.1"
                     /translation="MYTTLINANELQQLLHTDSRVLLLDVRFDLNDATFGQQVYDVGH
                     IAGAHYLHLDRELSGVVSGHNGRHPLPERKHLAHWLRNHGLTSDTQVVIYDQGSAMFA
                     ARAWALLRWLGHQAVAVLNGGLPAWLRAGGALSTELPKQPEVTDWQAGDSLLQVVSAD
                     EVMANIQSQEALVVDARSAERFDGEPHPLDKVSGHIPRAANLFFQQNLDAQQQFLSAE
                     ALQKLWQAVAHGKPLIHQCGSGVTACVNLLAQKHAGLGDGRLYVGSWSEWTSDNSRPI
                     VMAE"
     gene            1460..2194
                     /locus_tag="PK856_02820"
     CDS             1460..2194
                     /locus_tag="PK856_02820"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HRL01338.1"
                     /translation="MIAIIGGSGLTRLPEMNLSRRQIVRTPYGLPSSTLLFGNIGKLE
                     VVFLARHGFGHTIAPHKINYRANVWALHHVGARSIISAAAVYSMNEAIRPGSLVLPSD
                     ILDYSYDREHTFYQGEHQQVLHTAFDEPYDSGLKKMIAAAATKMSPPDIKHQAVYACL
                     QGPRFPSKAEVQRLIRDGADVFGMTGMPEAILAKELGMRYVHLCGVIAWAPGMEDTCT
                     TCSRIPSDETEYQAIAHIRALLAALN"
     gene            complement(2386..2808)
                     /locus_tag="PK856_02825"
     CDS             complement(2386..2808)
                     /locus_tag="PK856_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01339.1"
                     /translation="MKTSVIIGLLGVLCVSSVQAAVGDPKDSGQWQLIAKDAFGQKTF
                     MDTAHFQAGFPAQFLVKTDIVQCRSKFRMSTCTTVALFEADCKKGKLKYVRDMTRDVS
                     GEVVKEWENLRAKMVYPPSGTSMETLLIKACQRNKKLI"
     gene            complement(2809..3222)
                     /locus_tag="PK856_02830"
     CDS             complement(2809..3222)
                     /locus_tag="PK856_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRL01340.1"
                     /translation="MIITSQWVDGNCFVASNAMGHTVVMDGGATQGEKRGASPMELLL
                     MGMAGCSSIDVVSIAQKQRQDIVDCKARVEAVRADTTPRVFTKIHIHFIVSGRQLSEE
                     AIGKAVSLSADKYCSASIMLGKAAEVTHSFEVVEL"
     gene            complement(3276..3707)
                     /gene="rraA"
                     /locus_tag="PK856_02835"
     CDS             complement(3276..3707)
                     /gene="rraA"
                     /locus_tag="PK856_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009116083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HRL01341.1"
                     /translation="MPLYQAIQTADLIDLHPETPSCEVQFRRFGKHQRFSGRIRTVKC
                     FRDNGLVKQLANSEGKGAVMVVDGGGSLASALMGDQIAEAACKNNWAGVIIFGAIRDS
                     VAISRMEFGLKALGTNPRKSAKEGAGEIDVPICTVTKMVFW"
     gene            complement(3849..5273)
                     /gene="hemN"
                     /locus_tag="PK856_02840"
     CDS             complement(3849..5273)
                     /gene="hemN"
                     /locus_tag="PK856_02840"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HRL01342.1"
                     /translation="MEQVHNVHIPQRQVDFDRHLVETLAKNGPRYTSYPTADRFHAGF
                     NQQHYQQALQQRNVGALNRPLSLYVHVPFCNTICYYCGCNKIITKDTSRADDYIEYLG
                     RELDLIAPHLQGQHQLSQLHFGGGTPTFLSDDQLARVFEHIKCHFKFTPRGEYSIEID
                     PRKVSTDTVYRLRELGFNRMSVGIQDFDPKVQAAVNRVQSIEETQAVMTAARDAGFKS
                     ISVDVIYGLPHQTLASMQTTLEQVLALNPDRLALYHYAHMPHLFKPQRRIDNEDLPSS
                     AEKLDILQWAVEWLTEQGYVFIGMDHFAKPTDELALALEQGRLQRNFQGYSTHADCDL
                     IGIGVSSIGKVGVSYIQNQKDLPEYYASIDRGELPVMRGLLLKQDDLLRRNLIQTLMC
                     QFSLLFAPYEEQYGIRFKEYFAQEWQELLQQQQQGLLEITKHGLNVTGKGRFLIRNIA
                     MVFDQYLRQRQAEASEIPRYSQTV"
     gene            5535..>5889
                     /locus_tag="PK856_02845"
     CDS             5535..>5889
                     /locus_tag="PK856_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRL01343.1"
                     /translation="MQLKTLCSTCSLRELCLPVGLNPEEITQLDAVVRQSRRIKRGEY
                     LFRAGEAFTSLFAIRIGFFKTVIASQDGRDQVTGFSMSGELIGLDGISSGLHTCDAVA
                     LEDSEVCELPFDRMED"
     assembly_gap    5890..5989
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5990..6406
                     /locus_tag="PK856_02850"
     CDS             <5990..6406
                     /locus_tag="PK856_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRL01344.1"
                     /translation="VALEDSEVCELPFDRMEDLTQEIPSLQTHFYRLMSREIVRDQGV
                     MLLLGNMKSEERIAAFLLNLSQRLQKRGFAATDFILRMSREEIGSYLGLKLETVSRNL
                     SKFHQSGLINVDHKHIRINDIQKLKEMVSGCSHQAH"
     gene            complement(6457..7410)
                     /locus_tag="PK856_02855"
     CDS             complement(6457..7410)
                     /locus_tag="PK856_02855"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HRL01345.1"
                     /translation="MNTTPSKWPYTHKPWLMCLWLWMLLFSMACSWAGDEEGGFVWQI
                     EKTGQPKSYLVGTVHLGKTGSRLSPRLQTLLQQNQVLVVESNDADWEGSKGLILQQQM
                     GRLMAAEVPLKQSLGAKRLAAMNHSLAKNGSSIRLQGDAKMAPWMAWMLMATDYNMPG
                     YSMQAGIDVLLLQAAHKRKMRVVALETLEPMYVFKRIPNAVLLRGIDADLAYSEQGQK
                     EQLQMLQHYHQGDSKALIAKVLDAEDSVSHYPKQDRLFWRQWMFGDLLQQRNVAWMPK
                     LVGQIKQGPTVIAVGAAHLYGGSGLIVLLRERGYQVTPYRI"
BASE COUNT         1942 a         2041 c         1674 g         1897 t
ORIGIN      
        1 cccgctttgc ccgaagcaac aatgtacaac acccctgctg ctgcgatggc gcccaatact
       61 tgagacacta tgtagggcaa caaatccttg ccatcaaaac gaccgcccac aaacaagccc
      121 acagacaccg caggattaaa atgtccgcca gagatgtggc ccacagcata agccatggtc
      181 aacaccgtca aaccaaacgc caaggccaca cctgcaaagc ctatgcccaa ctcaggatag
      241 gccgcagcca aaaccgccga gccacaacca ccaaaaacca accaaaatgt gccaaaaaac
      301 tcggccaaat acttgttcat actgaatact cctcttgtgt catgcacatg ccacacacca
      361 ccgccttaca tatgattcga ggttacgcac cccttgtttg gcaatgtcgc ccagccctta
      421 atcaactgca aactgcattc atcactgcag ggcaataccc atcactcaat ttcaacaccc
      481 tattccgcca tcacaatggg gcggctgttg tcactggtcc actcactcca tgagcccaca
      541 tacaaacgcc catcccccaa accagcgtgt ttttgtgcca ataaattcac acaagccgtc
      601 acaccgctgc cacattggtg tatcaagggt ttaccatggg ccaccgcttg ccacagtttt
      661 tgcaaggctt ccgcacttaa gaactgttgt tgtgcatcca aattctgttg aaaaaacaaa
      721 ttcgcagcac gcggtatgtg gcctgagacc ttatccaacg gatgtggttc accatcaaag
      781 cgttcagcac tgcgcgcatc caccaccaaa gcctcttggg attgaatatt ggccatcacc
      841 tcatccgcac ttaccacttg caataaactg tcgcctgctt gccaatcggt cacttcaggc
      901 tgcttgggca actccgtgct taaggcacct cccgctcgta accaagcagg caagccacca
      961 ttcaataccg caacagcctg atgccccaac caacgcaaca aagcccacgc acgcgcagca
     1021 aacatggcag aaccttggtc gtatatcacc acttgtgtat ccgaagtcag gccatggttg
     1081 cgcaaccaat gcgccaaatg tttgcgctca ggcaagggat ggcggccatt gtgaccactg
     1141 accacacccg acaattcacg atccaaatgc aaataatggg cacccgcaat gtgaccgacg
     1201 tcataaacct gttgtccaaa tgtggcatca ttcaaatcaa agcgcacatc aagcaacaaa
     1261 acgcggctgt ctgtgtgcaa taactgttgt aactcattgg cattgatcag tgtggtatac
     1321 atactatgct ccttttatgc agatgcgctg tgtcagctta cgctagccca agcccagcat
     1381 tttgtccagt gggcggcggg cgaatttgcc ctagtttgcc acttatggct acaatcgcca
     1441 caagcttgga ggaaacacca tgattgcaat tatcggtggc agcggcttga cccgcttgcc
     1501 agaaatgaat ttgagtcgtc gccaaattgt gcgcacgcct tatggcttac ccagctccac
     1561 tttattgttt ggcaacattg gcaaactgga ggtagtgttt ttggcgcggc atggcttcgg
     1621 tcataccatc gcgccccaca aaatcaatta ccgcgccaat gtgtgggctt tgcaccatgt
     1681 aggcgcacgc tccatcatct cggcagcggc ggtgtacagc atgaacgaag ccatacgccc
     1741 aggcagcttg gttttgccct cagacatact cgattacagt tacgaccgtg aacacacgtt
     1801 ttaccaaggt gagcaccaac aggtattgca cacggcgttt gacgagcctt acgacagcgg
     1861 cctgaagaaa atgatagccg ctgctgccac caagatgtca cctcccgaca tcaaacacca
     1921 agcggtgtat gcttgcttac aagggccgcg ttttcccagc aaagctgagg tacagcgttt
     1981 gatacgtgac ggtgccgatg ttttcggcat gacgggcatg cctgaagcga ttttggccaa
     2041 ggaattgggc atgcgctatg tgcatttgtg tggcgtcatc gcttgggcac ctggcatgga
     2101 agacacttgc accacctgca gccgcatacc gagtgacgaa accgaatacc aagccatcgc
     2161 ccacattcgg gcattgctgg ctgcattaaa ctgatttgtc atcagtaaat acttgctttt
     2221 aatgtttaca ggccgcttta aagcggcctg taaataaaca gacaagtgtt actccagcac
     2281 gtctgagcat cgaaacactc tttgctgctt aagctcaaac aacacccttt attcagtgtt
     2341 tgcaacctct aatgcattac aggccacatt aagcggcctg taaactcata tcagtttttt
     2401 attgcgttgg caggccttga ttaacaaggt ttccattgaa gtcccactcg gtggatacac
     2461 catcttggcc ctaaggtttt cccactcttt caccacttcc ccactgacat cacgggtcat
     2521 gtcgcgcaca tatttgagtt tgcctttttt acaatcggct tcaaacaagg ccacggtggt
     2581 gcaagtgctc atccgaaact tgctgcggca ttggacaatg tcggttttca ccaaaaactg
     2641 cgcgggaaaa cctgcttgaa agtgggctgt gtccatgaag gttttttgac caaaggcatc
     2701 tttggcaatc aactgccatt gcccactgtc tttgggatca cccactgcgg cctgtacgct
     2761 ggaaacacac aacacgccca ataagcctat gatgacagag gttttcattt acagctctac
     2821 cacctcaaaa ctgtgggtca cttccgcggc tttgcccaac ataatcgaag ccgagcagta
     2881 tttgtcggca gacaggctca cggctttgcc tatggcctct tcactcaatt gacggccact
     2941 gacgatgaag tggatgtgga ttttggtgaa cacgcgcggt gtggtgtcgg cgcgcacagc
     3001 ttccactcgg gctttacaat caacgatgtc ttggcgctgt ttttgggcaa tgctgaccac
     3061 atcaatgcta gagcagcccg ccatgcccat taacaacaac tccattgggc tggcgccccg
     3121 tttttcacct tgggtggcgc cgccatccat caccacagtg tgtcccatgg cattgctggc
     3181 cacgaaacaa ttgccgtcta cccattgcga ggtaatgatc atgttctgtc tttcttcaat
     3241 ctgtttatca atatcttaag ccaaaggctc ggcactcacc aaaataccat cttcgtcact
     3301 gtacaaatag gcacgtcgat ttcgcccgcg ccctctttcg cacttttgcg cggattggtg
     3361 cccaaagcct ttaagccaaa ttccatgcgg ctgatggcca cactgtcgcg aatggcccca
     3421 aagatgatga cgcctgccca gttgttttta caggccgctt cggcaatttg gtcgcccatc
     3481 aaggcactgg ccaaagaacc tccaccatcc accaccatca ccgcgccttt gccctcactg
     3541 ttggccaatt gcttgaccaa gccattgtcg cgaaaacact tcaccgtgcg gatgcggcct
     3601 gaaaagcgtt ggtgcttacc aaaacgccga aactgcacct cacaagaagg ggtttcaggg
     3661 tgtaaatcaa tcaaatcggc ggtttgtatg gcttggtaca gtggcattgt gtgctcccgt
     3721 ctggatagaa aaccatttca acagtgtttt ttcaacgcgc agcaaacgcg gtgcgacgca
     3781 agtttgcatc aacaagcgcc caaagtaaag gcgtccacaa gatggggacg cctttagcaa
     3841 aaatcatgtt aaaccgtttg cgaataccgc ggtatctcgc tggcttcagc ctggcgttgg
     3901 cgcagatatt ggtcaaacac catggcaatg ttgcggatta aaaaccgacc tttgccagtg
     3961 acgttcaggc cgtgtttggt gatttccaac aagccttgtt gctgttgctg taacaattcc
     4021 tgccactctt gggcgaaata ctctttaaaa cgaatgccat attgttcttc atacggtgca
     4081 aacaacaaac tgaattgaca catcaaggtt tgtatcaagt tgcggcgcag caagtcatct
     4141 tgcttgagca ataagccacg catcacaggc aactcgcctc ggtctatgct ggcataatac
     4201 tcaggcaagt ctttttggtt ttgaatgtaa ctcacgccga ctttgcctat gctcgacacg
     4261 cctatgccta tcaaatcgca atcggcatgg gtggaatagc cttggaaatt tcgctgcaaa
     4321 cgcccttgct ccaaagccaa agccaattca tcggtgggtt tggcaaaatg gtccatgccg
     4381 atgaacacat agccttgctc agtcaaccac tccaccgccc actgcaaaat atccagtttt
     4441 tcagcgctgg agggcaagtc ctcattgtca atgcgccgtt gcggcttgaa caagtgcggc
     4501 atgtgcgcat agtggtataa ggccaaacgg tctgggttta aggccaaaac ctgctccaat
     4561 gtggtttgca tactcgccaa agtttggtgc ggcaagccat aaatcacgtc cacgctgatg
     4621 gatttgaagc cagcatctct cgctgccgtc atcaccgctt gggtttcttc tatgctttga
     4681 actcgattga cagcggcctg tactttggga tcgaagtctt gtatgcccac gctcatgcgg
     4741 ttaaaaccca attcacgcaa acggtaaacc gtgtcagtgc tgaccttgcg cgggtctatt
     4801 tcaatggaat actcacctct gggggtgaat ttgaagtggc acttgatgtg ttcaaacact
     4861 cgggccaatt ggtcatcact gagaaatgtt ggtgtgccac caccaaaatg caattgcgac
     4921 aactgatgtt gcccttgtaa atgcggtgca atcaaatcca actcacgccc caaatactca
     4981 atatagtcat cggcacggct ggtgtctttg gtgatgattt tgttgcaacc acagtaatag
     5041 caaatggtat tgcaaaatgg cacatgcaca tacaaagaca agggacgatt gagcgcaccc
     5101 acattgcgtt gctgcaaagc ttgttggtaa tgctgctggt tgaaacctgc gtgaaaacgg
     5161 tctgctgtgg gataggaagt gtatcttggg ccatttttgg ccaaggtttc taccaaatgc
     5221 cgatcaaagt ctacttgacg ctgtggaatg tgcacgttgt gaacctgttc gattttgtca
     5281 ttataaacaa attaataaga acgagtttgc cacttcctag cttaacaagg tttgacttat
     5341 atcaatacat catagtaaac aagcaatttg taaggttttt ttgattcaca tcatgtactt
     5401 agtaaagtag aatagtaaaa gtatcatatc aagtcgttag aatgttaatt cggtaattgc
     5461 atccaatcaa aacacatgca atgttaaagc cgtgatttga tactttttgt ccatcattta
     5521 tgaaaagtct ctgcatgcaa ctgaaaacat tgtgctcgac ctgctctttg cgcgaactgt
     5581 gtttacctgt tgggttaaac ccagaagaaa tcacccaatt ggatgcggtc gtgcgccaaa
     5641 gtcgccgcat caaacggggc gaatacctat tccgtgctgg tgaagcgttt acctctttat
     5701 ttgccatccg cattggcttt ttcaaaaccg tgattgccag ccaagacggg cgcgaccaag
     5761 tgacgggctt ttcaatgtcg ggcgagctca tcggtttgga tggcatctct tctggcctgc
     5821 acacgtgtga cgcagtggcc ttggaagaca gtgaagtgtg cgaattgcca tttgaccgca
     5881 tggaagactn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng tggccttgga
     6001 agacagtgaa gtgtgcgaat tgccatttga ccgcatggaa gacttgaccc aagaaattcc
     6061 ttctttgcaa acgcattttt accgcttgat gagccgtgaa atcgtacgcg atcaaggcgt
     6121 gatgctgtta ttgggcaata tgaaatccga agaacgcatt gcggctttct tattgaattt
     6181 gtcacagcgc ttgcaaaaac gtggctttgc cgccaccgat tttattttgc gcatgtcacg
     6241 tgaggaaatt ggcagctatt tgggtttgaa attggaaacc gtcagtcgca atttgtcgaa
     6301 gttccaccaa agcggcttga tcaatgtcga tcacaagcac atccgcatca atgacattca
     6361 aaaattaaaa gaaatggttt caggatgtag ccatcaagca cactgatggt gttttgattt
     6421 aacacgacag cgccctgttt acaggacgct gctgtgttaa atacggtacg gtgtcacctg
     6481 atatcctcgc tctctgagca acacaatcaa accactgcca ccatacaaat gagcagcccc
     6541 caccgcaatg acggttggcc cttgtttgat ttgtccaaca agcttgggca tccacgccac
     6601 attgcgctgc tgcaacaaat ccccaaacat ccattgtcgc caaaacaacc tgtcttgctt
     6661 aggataatgg ctcacgctgt cttcagcatc caaaacctta gcaatcaatg ccttgctatc
     6721 gccttgatgg taatgctgca acatttgcaa ttgctctttt tgcccttgct cactgtaagc
     6781 caaatcggca tcaatgccac ggagcaacac cgcattcgga atgcgtttga acacatacat
     6841 cggctccaag gtttccaaag ccaccacacg catttttcgt ttgtgagcgg cctgtaacaa
     6901 caacacatct atgcctgctt gcatcgaata gcctggcata ttgtaatcag tcgccatcag
     6961 catccaagcc atccaaggcg ccatcttggc atcgccctgt aaacggatgc tgctgccatt
     7021 tttggccaat gagtggttca tggctgccaa gcgtttcgct cccaagcttt gcttcagcgg
     7081 cacttccgcc gccatcagcc tccccatttg ttgctgcaat atcaggcctt tgctgccttc
     7141 ccaatctgca tcattcgatt ccaccaccaa tacttggttt tgctgcaaca aagtctgcaa
     7201 acgaggcgac aaacggctgc ctgttttgcc caaatgcaca gtaccaacca aataggattt
     7261 gggttgacct gttttctcaa tttgccaaac aaaaccgcct tcttcatccc ccgcccaact
     7321 gcaagccatg ctgaacagca acatccacaa ccacaggcac atcaaccaag gtttgtgcgt
     7381 gtacggccat tttgatgggg tggtgttcat ttgatactcc taaaaaatgt agactccatg
     7441 caaaccttct gtcgattttt tatctgtcgc cccttgacaa aagccaagac aaacccatat
     7501 gggatacata agagaacttt atacaggagt gtgcaatgcg ttttgaacag ttaacacagc
     7561 gtttccagca agccttacag gacgcccaaa gtttggcgat gcgcaatgat aacggctatc
     7621 tggaagcggt gcatgtctta aaagtattac tcga
//
LOCUS       DAOMVK010000049         7598 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_2540_length_7598_cov_3.601750, whole genome shotgun sequence.
ACCESSION   DAOMVK010000049 DAOMVK010000000
VERSION     DAOMVK010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 7598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7598
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2540_length_7598_cov_3.601750"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..581
                     /locus_tag="PK856_02860"
     CDS             84..581
                     /locus_tag="PK856_02860"
                     /inference="COORDINATES: protein motif:HMM:NF014639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HRL01346.1"
                     /translation="MYSKKHLWLIVLVSAVVIAAALYSQYVLKMDPCPLCIFQRVAVI
                     FVGLFALLMALLPQRKSGMGLFSAVLISIPALIGLGIAIRQRYIQGLPPSEVPACGPG
                     LDFMMDTLPLQGVIQTVLSGSGECAAVEYVLGVPLPIWSALFFVFAILVAWGGWFKFR
                     RGRRY"
     gene            600..728
                     /locus_tag="PK856_02865"
     CDS             600..728
                     /locus_tag="PK856_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01347.1"
                     /translation="MKLALLAVTVLLAVSACSSTGGGSSNQVYGEVSGGVGYTTSK"
     gene            complement(790..2802)
                     /locus_tag="PK856_02870"
     CDS             complement(790..2802)
                     /locus_tag="PK856_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRL01348.1"
                     /translation="MNSDPYAQFENLDDPYVAQYAHSAHQRTLDAFVDDATMAMADDM
                     LAQIQNDKQIPFCQEHRARMYHHFQSADYSKGVYRVCSAASFRSGLPDWDILFAVEAF
                     DEVLGDDVYLNHVTHCTLAPTRALLELSSGGGDAAYTLEFDLTEKRIVDGGFHFPQGK
                     NHIAWRDENSVWVCPAWHEGQLTHSAYPREVWMLERGHGMDEAIPVLQADVDDMLVAA
                     CRYLDPQGRDLDVIEVAHDFYRKTYYWVDEDIQAHALKLPDTCDLIGYLGGQLLVWLK
                     SDWQRANQRYSSGSLLAVKLHKGELGQASVLFAPDAHSVLESVETTRQFVVMHVLNNV
                     QSELRAQQFKQGAWHEAHAPSLPKGTIELVDQPYGGDVVYAAISDFTTPLTLLCVDFS
                     LQDVAVLRKQPKQFDAKNMLTQQLWANSADGTAIPYFHVGTPSEQPVPTIVYVYGGFA
                     VAELPHYLGLLGRHWLSLGYAFVVANVRGGGEFGPAWHQAAILKNKHKSVEDLLAVVH
                     DLHTRGLSTAKQTGLQGGSNGGLMALAAMVDEPDSVGAVVAEVPLADMLRYPHLSSGA
                     SWLAEFGDPHSEDEDEQRALYDLSPYHRIAHQRTYPATLLTTSLSDDRVHPAHAFKTY
                     ARLQQLEQASWLYCPEDGGHTGNTTQEELADETAVIMTFFAKQLAG"
     gene            2990..3193
                     /locus_tag="PK856_02875"
     CDS             2990..3193
                     /locus_tag="PK856_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRL01349.1"
                     /translation="MFVCICTGVTDTEIKQSIADGCRDMASLQAELEVATCCGRCGDM
                     CEKLLSEAHHVHTRPIEIAIQVA"
     gene            complement(3199..3675)
                     /gene="bfr"
                     /locus_tag="PK856_02880"
     CDS             complement(3199..3675)
                     /gene="bfr"
                     /locus_tag="PK856_02880"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HRL01350.1"
                     /translation="MQGSKLVKRELNKVLAVSLIAINQYFLHARMLKNWGVEALGKSI
                     YKQSIEEMKMSDDIIERVLLLEGLPNLQDLGKLLLGENVKEILECDLQLEQIKHTALI
                     EAIKVCEAESDFVSREMLLKLKDENEEHTDWLEMQLNLIGEMGLENFIQQSVGEND"
     gene            complement(3695..3805)
                     /locus_tag="PK856_02885"
                     /pseudo
     CDS             complement(3695..3805)
                     /locus_tag="PK856_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003786850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
     gene            3794..3904
                     /locus_tag="PK856_02890"
                     /pseudo
     CDS             3794..3904
                     /locus_tag="PK856_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003786850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
     gene            3924..4400
                     /gene="bfr"
                     /locus_tag="PK856_02895"
     CDS             3924..4400
                     /gene="bfr"
                     /locus_tag="PK856_02895"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HRL01351.1"
                     /translation="MQGSKLVKRELNKVLAVSLIAINQYFLHARMLKNWGVEALGKSI
                     YKQSIEEMKMSDDIIERVLLLEGLPNLQDLGKLLLGENVKEILECDLQLEQIKHTALI
                     EAIKVCEAESDFVSREMLLKLKDENEEHTDWLEMQLNLIGEMGLENFIQQSVGEND"
     gene            complement(4406..4609)
                     /locus_tag="PK856_02900"
     CDS             complement(4406..4609)
                     /locus_tag="PK856_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRL01352.1"
                     /translation="MFVCICTGVTDTEIKQSIADGCRDMASLQAELEVATCCGRCGDM
                     CEKLLSEAHHVHTRPIEIAIQVA"
     gene            4797..6809
                     /locus_tag="PK856_02905"
     CDS             4797..6809
                     /locus_tag="PK856_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRL01353.1"
                     /translation="MNSDPYAQFENLDDPYVAQYAHSAHQRTLDAFVDDATMAMADDM
                     LAQIQNDKQIPFCQEHRARMYHHFQSADYSKGVYRVCSAASFRSGLPDWDILFAVEAF
                     DEVLGDDVYLNHVTHCTLAPTRALLELSSGGGDAAYTLEFDLTEKRIVDGGFHFPQGK
                     NHIAWRDENSVWVCPAWHEGQLTHSAYPREVWMLERGHGMDEAIPVLQADVDDMLVAA
                     CRYLDPQGRDLDVIEVAHDFYRKTYYWVDEDIQAHALKLPDTCDLIGYLGGQLLVWLK
                     SDWQRANQRYSSGSLLAVKLHKGELGQASVLFAPDAHSVLESVETTRQFVVMHVLNNV
                     QSELRAQQFKQGAWHEAHAPSLPKGTIELVDQPYGGDVVYAAISDFTTPLTLLCVDFS
                     LQDVAVLRKQPKQFDAKNMLTQQLWANSADGTAIPYFHVGTPSEQPVPTIVYVYGGFA
                     VAELPHYLGLLGRHWLSLGYAFVVANVRGGGEFGPAWHQAAILKNKHKSVEDLLAVVH
                     DLHTRGLSTAKQTGLQGGSNGGLMALAAMVDEPDSVGAVVAEVPLADMLRYPHLSSGA
                     SWLAEFGDPHSEDEDEQRALYDLSPYHRIAHQRTYPATLLTTSLSDDRVHPAHAFKTY
                     ARLQQLEQASWLYCPEDGGHTGNTTQEELADETAVIMTFFAKQLAG"
     gene            complement(6871..6999)
                     /locus_tag="PK856_02910"
     CDS             complement(6871..6999)
                     /locus_tag="PK856_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01354.1"
                     /translation="MKLALLAVTVLLAVSACSSTGGGSSNQVYGEVSGGVGYTTSK"
     gene            complement(7018..7515)
                     /locus_tag="PK856_02915"
     CDS             complement(7018..7515)
                     /locus_tag="PK856_02915"
                     /inference="COORDINATES: protein motif:HMM:NF014639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HRL01355.1"
                     /translation="MYSKKHLWLIVLVSAVVIAAALYSQYVLKMDPCPLCIFQRVAVI
                     FVGLFALLMALLPQRKSGMGLFSAVLISIPALIGLGIAIRQRYIQGLPPSEVPACGPG
                     LDFMMDTLPLQGVIQTVLSGSGECAAVEYVLGVPLPIWSALFFVFAILVAWGGWFKFR
                     RGRRY"
BASE COUNT         1897 a         1902 c         1902 g         1897 t
ORIGIN      
        1 taggctccat catgatcagt gaaatgactg tggcgggtga tgaagcggcc tgaaacccct
       61 aactgacttc caaaggaaga ctgatgtaca gtaaaaaaca tttgtggctc atcgtgctgg
      121 tatcggccgt ggtgattgcc gctgcactct attcgcaata cgtattgaaa atggatcctt
      181 gccctttgtg tatcttccaa cgggtggcag tgatttttgt gggcttgttt gccttgttga
      241 tggctttgtt gccacaacgc aaaagcggca tgggtttgtt ttcagcagtg ctcatcagca
      301 ttcctgcctt aatcggcttg ggcatcgcca tccgtcaacg ctatatccaa ggtttgccac
      361 cgtctgaagt gcccgcttgt ggacctggtt tggatttcat gatggatacc ttgcctttgc
      421 aaggcgtgat acaaaccgtg ttgtcgggct cgggtgagtg tgctgcagtg gaatatgttt
      481 tgggcgtgcc tttgccaatt tggagcgctt tgttctttgt gtttgccatc ttggtggcat
      541 ggggcggttg gttcaagttt cgtcgcggcc gccgttattg atttaaaaaa ggaaatgaca
      601 tgaaattagc attattggct gtgacagttt tgttggcggt gtctgcttgc agcagcacag
      661 gtggcggctc gtctaatcaa gtgtatggcg aagtgtcggg tggtgtgggt tacaccactt
      721 ctaaataagc gttttgacat aataagcggc ctgtaaaaat ttacaggccg ctttttttat
      781 ttataggatt taacctgcca attgtttggc aaagaaagtc atgatgacag cggtttcatc
      841 cgccaattct tcttgggtgg tgttgcctgt gtggccgcca tcttcagggc aatacagcca
      901 tgaggcttgt tccaattgtt gcaagcgagc ataagttttg aaggcgtggg cagggtggac
      961 acggtcatcg ctcaagctgg tggtgagtaa ggtggcagga tacgtgcgct ggtgggcaat
     1021 gcggtggtaa ggagacaaat catacaaagc gcgttgctcg tcttcgtctt cactgtgtgg
     1081 gtcgccaaac tcggccaacc aagaagcgcc gctggacaaa tgtggatagc gcaacatgtc
     1141 ggccaaaggc acttctgcta ccactgcacc cacgctgtca ggctcatcca ccatggctgc
     1201 caatgccatc aggccaccat tgctgccacc ttgtaagcct gtttgcttgg cagtgcttaa
     1261 gccgcgggtg tgtaaatcgt gtaccacagc cagcaagtct tctaccgatt tgtgtttgtt
     1321 tttgaggatg gcggcttggt gccaagcggg gccaaactcg ccgccgccgc gcacattggc
     1381 caccacaaaa gcataaccca agctcaacca atggcggccg agcaagccca aataatgcgg
     1441 caattcggcc acggcaaatc caccgtagac atacacgatg gtgggtacag gctgctcgct
     1501 cggtgtgccg acgtgaaaat acggaatggc agtgccatcg gcgctgttgg cccacaattg
     1561 ttgcgtgagc atgtttttgg catcaaattg tttgggctgt ttgcgcaaca ccgccacatc
     1621 ttgtaaggaa aaatcgacac acagcaaggt caaaggcgtg gtgaaatcgc tgatggcggc
     1681 atataccaca tcgccaccgt aaggttggtc aaccagttcg atggtgcctt tgggcaagga
     1741 tggggcatgg gcttcatgcc aagcgccttg tttgaattgc tgcgcgcgca attcgctttg
     1801 cacattgttc aatacatgca tcaccacaaa ctggcgcgtg gtttccaccg attccagcac
     1861 gctgtgtgca tcgggtgcaa acaacacgct ggcttgcccc aattcacctt tgtgcagctt
     1921 cactgcgagc aaactgccgc tgctgtaacg ctggttggca cgttgccaat cgcttttgag
     1981 ccacaccagt aattgtccac ccaaatagcc gattaaatcg caggtgtctg gcagttttaa
     2041 ggcatgggct tggatgtctt catcgaccca gtaataggtt ttgcggtaga aatcgtgcgc
     2101 aacttcgatg acgtccaaat cgcgcccttg tgggtcgaga tagcgacaag cggccaccaa
     2161 catgtcgtcc acatcggcct gtaacacagg aatggcttca tccatgccat gaccgcgctc
     2221 cagcatccac acctcacgcg ggtaagcact gtgggtgagt tggccttcgt gccaagcagg
     2281 gcaaacccac acgctgtttt catcgcgcca cgcaatgtgg tttttacctt gcggaaaatg
     2341 aaagccgcca tcgacgatgc gtttttcagt caaatcaaat tcgagggtat aagccgcatc
     2401 gccgccaccg ctgctcagtt ccaataaggc gcgagtgggg gcgagggtgc agtgggtaac
     2461 gtggttcaaa tacacatcgt cgcccaaaac ctcgtcaaag gcttctacgg caaacaagat
     2521 gtcccaatcg ggcaggccag aacggaagct cgcggcgctg cacacgcggt acacgccttt
     2581 gctgtagtcg gcgctttgga aatggtggta catgcgtgcg cggtgttctt ggcaaaaggg
     2641 aatttgtttg tcgttttgga tttgcgccag catgtcgtct gccatggcca tcgtggcatc
     2701 atcaacaaac gcatctaaag tgcgttggtg ggcggaatgg gcatactggg ccacatatgg
     2761 gtcgtctagg ttttcaaact gagcgtaagg atctgaattc atcgcagcat gggagtgtca
     2821 acaaggccgt tattatagcc tgaatcggca gaggctttat ctgcttgatt gtgtgcgcaa
     2881 ttcaattaaa tccgactaaa tttgtaaaga ttaccgaatg cacttgcaaa tgatagggac
     2941 tatcattatt atagcgattc aatatgacga agattttttg gagactgacg tgtttgtttg
     3001 catttgcaca ggtgtgaccg atactgaaat caaacaaagc atcgccgatg gttgccgcga
     3061 tatggcgagt ttacaggccg agttggaagt agcgacttgc tgtggtcgct gtggtgatat
     3121 gtgtgaaaaa ttattgagcg aggcgcacca tgtgcacact cgcccaatcg agattgcgat
     3181 acaagtggct taagccgctc agtcgttttc gcccacagat tgctgaataa agttttccaa
     3241 gcccatttca ccaattaaat tcaactgcat ttccaaccag tcggtgtgtt cttcgttttc
     3301 atctttcaat ttgagcagca tttcacggct gacaaaatcg ctttctgctt cacaaacttt
     3361 aatcgcttca atcaaagccg tgtgtttgat ttgttccagc tgtaaatcac attccaaaat
     3421 ttctttgacg ttttcaccga gcaacaattt gcccaagtct tgcaaattcg gcaagccttc
     3481 cagcaacaac acacgctcga tgatgtcatc agacatcttc atttcttcaa tcgattgttt
     3541 gtaaatcgac ttgcccaatg cctctacacc ccagtttttc aacatgcgtg catgtaagaa
     3601 atactgattg atggcaatca aagacacggc caagactttg ttcaattcac gtttgacgag
     3661 tttgctgcct tgcatgatgt gtgtcctttc gcgctcagat ttggctttgc aaatagtttt
     3721 gcaagcccat caattcgatt aagcccaatt gttgttcgag ccaatgtgcg tggtcttctt
     3781 cggtgtcgcg caattgttgc aacaattgcg cgacaccgaa gaagaccacg cacattggct
     3841 cgaacaacaa ttgggcttaa tcgaattgat gggcttgcaa aactatttgc aaagccaaat
     3901 ctgagcgcga aaggacacac atcatgcaag gcagcaaact cgtcaaacgt gaattgaaca
     3961 aagtcttggc cgtgtctttg attgccatca atcagtattt cttacatgca cgcatgttga
     4021 aaaactgggg tgtagaggca ttgggcaagt cgatttacaa acaatcgatt gaagaaatga
     4081 agatgtctga tgacatcatc gagcgtgtgt tgttgctgga aggcttgccg aatttgcaag
     4141 acttgggcaa attgttgctc ggtgaaaacg tcaaagaaat tttggaatgt gatttacagc
     4201 tggaacaaat caaacacacg gctttgattg aagcgattaa agtttgtgaa gcagaaagcg
     4261 attttgtcag ccgtgaaatg ctgctcaaat tgaaagatga aaacgaagaa cacaccgact
     4321 ggttggaaat gcagttgaat ttaattggtg aaatgggctt ggaaaacttt attcagcaat
     4381 ctgtgggcga aaacgactga gcggcttaag ccacttgtat cgcaatctcg attgggcgag
     4441 tgtgcacatg gtgcgcctcg ctcaataatt tttcacacat atcaccacag cgaccacagc
     4501 aagtcgctac ttccaactcg gcctgtaaac tcgccatatc gcggcaacca tcggcgatgc
     4561 tttgtttgat ttcagtatcg gtcacacctg tgcaaatgca aacaaacacg tcagtctcca
     4621 aaaaatcttc gtcatattga atcgctataa taatgatagt ccctatcatt tgcaagtgca
     4681 ttcggtaatc tttacaaatt tagtcggatt taattgaatt gcgcacacaa tcaagcagat
     4741 aaagcctctg ccgattcagg ctataataac ggccttgttg acactcccat gctgcgatga
     4801 attcagatcc ttacgctcag tttgaaaacc tagacgaccc atatgtggcc cagtatgccc
     4861 attccgccca ccaacgcact ttagatgcgt ttgttgatga tgccacgatg gccatggcag
     4921 acgacatgct ggcgcaaatc caaaacgaca aacaaattcc cttttgccaa gaacaccgcg
     4981 cacgcatgta ccaccatttc caaagcgccg actacagcaa aggcgtgtac cgcgtgtgca
     5041 gcgccgcgag cttccgttct ggcctgcccg attgggacat cttgtttgcc gtagaagcct
     5101 ttgacgaggt tttgggcgac gatgtgtatt tgaaccacgt tacccactgc accctcgccc
     5161 ccactcgcgc cttattggaa ctgagcagcg gtggcggcga tgcggcttat accctcgaat
     5221 ttgatttgac tgaaaaacgc atcgtcgatg gcggctttca ttttccgcaa ggtaaaaacc
     5281 acattgcgtg gcgcgatgaa aacagcgtgt gggtttgccc tgcttggcac gaaggccaac
     5341 tcacccacag tgcttacccg cgtgaggtgt ggatgctgga gcgcggtcat ggcatggatg
     5401 aagccattcc tgtgttacag gccgatgtgg acgacatgtt ggtggccgct tgtcgctatc
     5461 tcgacccaca agggcgcgat ttggacgtca tcgaagttgc gcacgatttc taccgcaaaa
     5521 cctattactg ggtcgatgaa gacatccaag cccatgcctt aaaactgcca gacacctgcg
     5581 atttaatcgg ctatttgggt ggacaattac tggtgtggct caaaagcgat tggcaacgtg
     5641 ccaaccagcg ttacagcagc ggcagtttgc tcgcagtgaa gctgcacaaa ggtgaattgg
     5701 ggcaagccag cgtgttgttt gcacccgatg cacacagcgt gctggaatcg gtggaaacca
     5761 cgcgccagtt tgtggtgatg catgtattga acaatgtgca aagcgaattg cgcgcgcagc
     5821 aattcaaaca aggcgcttgg catgaagccc atgccccatc cttgcccaaa ggcaccatcg
     5881 aactggttga ccaaccttac ggtggcgatg tggtatatgc cgccatcagc gatttcacca
     5941 cgcctttgac cttgctgtgt gtcgattttt ccttacaaga tgtggcggtg ttgcgcaaac
     6001 agcccaaaca atttgatgcc aaaaacatgc tcacgcaaca attgtgggcc aacagcgccg
     6061 atggcactgc cattccgtat tttcacgtcg gcacaccgag cgagcagcct gtacccacca
     6121 tcgtgtatgt ctacggtgga tttgccgtgg ccgaattgcc gcattatttg ggcttgctcg
     6181 gccgccattg gttgagcttg ggttatgctt ttgtggtggc caatgtgcgc ggcggcggcg
     6241 agtttggccc cgcttggcac caagccgcca tcctcaaaaa caaacacaaa tcggtagaag
     6301 acttgctggc tgtggtacac gatttacaca cccgcggctt aagcactgcc aagcaaacag
     6361 gcttacaagg tggcagcaat ggtggcctga tggcattggc agccatggtg gatgagcctg
     6421 acagcgtggg tgcagtggta gcagaagtgc ctttggccga catgttgcgc tatccacatt
     6481 tgtccagcgg cgcttcttgg ttggccgagt ttggcgaccc acacagtgaa gacgaagacg
     6541 agcaacgcgc tttgtatgat ttgtctcctt accaccgcat tgcccaccag cgcacgtatc
     6601 ctgccacctt actcaccacc agcttgagcg atgaccgtgt ccaccctgcc cacgccttca
     6661 aaacttatgc tcgcttgcaa caattggaac aagcctcatg gctgtattgc cctgaagatg
     6721 gcggccacac aggcaacacc acccaagaag aattggcgga tgaaaccgct gtcatcatga
     6781 ctttctttgc caaacaattg gcaggttaaa tcctataaat aaaaaaagcg gcctgtaaat
     6841 ttttacaggc cgcttattat gtcaaaacgc ttatttagaa gtggtgtaac ccacaccacc
     6901 cgacacttcg ccatacactt gattagacga gccgccacct gtgctgctgc aagcagacac
     6961 cgccaacaaa actgtcacag ccaataatgc taatttcatg tcatttcctt ttttaaatca
     7021 ataacggcgg ccgcgacgaa acttgaacca accgccccat gccaccaaga tggcaaacac
     7081 aaagaacaaa gcgctccaaa ttggcaaagg cacgcccaaa acatattcca ctgcagcaca
     7141 ctcacccgag cccgacaaca cggtttgtat cacgccttgc aaaggcaagg tatccatcat
     7201 gaaatccaaa ccaggtccac aagcgggcac ttcagacggt ggcaaacctt ggatatagcg
     7261 ttgacggatg gcgatgccca agccgattaa ggcaggaatg ctgatgagca ctgctgaaaa
     7321 caaacccatg ccgcttttgc gttgtggcaa caaagccatc aacaaggcaa acaagcccac
     7381 aaaaatcact gccacccgtt ggaagataca caaagggcaa ggatccattt tcaatacgta
     7441 ttgcgaatag agtgcagcgg caatcaccac ggccgatacc agcacgatga gccacaaatg
     7501 ttttttactg tacatcagtc ttcctttgga agtcagttag gggtttcagg ccgcttcatc
     7561 acccgccaca gtcatttcac tgatcatgat ggagccta
//
LOCUS       DAOMVK010000050         7512 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_2588_length_7512_cov_3.350677, whole genome shotgun sequence.
ACCESSION   DAOMVK010000050 DAOMVK010000000
VERSION     DAOMVK010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 7512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7512
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2588_length_7512_cov_3.350677"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..>679)
                     /locus_tag="PK856_02920"
     CDS             complement(149..>679)
                     /locus_tag="PK856_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01356.1"
                     /translation="QAWQLGQSNWLFGSFDKINYTFVFHPHNIFLQWWTEYGVLGIYL
                     AIISIVFVLAILKQSCVLPPLSVAGIISISVNAMLSGAMVYPHSQIFNMIYFAYVVSL
                     LPQIEKPLFWEKQRKYIVGLVIVLVGLLLFYQHSNFLIWNGTSELYQSSGSHDSIVAP
                     YWWQYGGSEWLGNMNE"
     assembly_gap    680..709
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<710..1510)
                     /locus_tag="PK856_02925"
     CDS             complement(<710..1510)
                     /locus_tag="PK856_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01357.1"
                     /translation="MKNLFSHIVLICSLVYTFFVFSGYNIGWGHFPYDEWRLLANSLL
                     VIIVCIALKPEIWICNVVIVRLIGPLVILFIIVIIYILFIPKQINSISFLLVYSLLYF
                     AIFIYSFVFQINRSKEFYLIILSCMPLLTVMWLPIESILMYMFPEEIASKAWLGNFLN
                     KRQYDDAILPFLFILALLKVPKNFEIFRWALWVLILSGSIQNGSRAVIFSLIFGLIFA
                     AIWYRSYRWSRIFIIGTISAVLLALVFTFLQNQPALTALQRIDDSGRGH"
     gene            complement(1568..3082)
                     /gene="ubiB"
                     /locus_tag="PK856_02930"
     CDS             complement(1568..3082)
                     /gene="ubiB"
                     /locus_tag="PK856_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HRL01358.1"
                     /translation="MKWLKRFRLIVHTVWYFGLINLLDGQLRHPLLRKLLSCLPESKE
                     TLEYSNAVRLRLALENLGPIFVKLGQVLSTRPDVVGPEYAFELAKLQDRVPAFDGGIA
                     IAQIEKAFGKPIEQIYYAFEREPIASASIAQVHQAFLFNEDGTQGKKVAVKVLRPDIK
                     PVIEQDLALMHWGAVWLEKLFRDGKRLKPREVVQEFDKYLHDELDLMHEAANASQLGR
                     QFENSPMLIVPKVYFDFCSRSVMTMEWMDGTPISDVDTLVAKGIDLKKLARFGVEIFF
                     TQVFKNGFFHADMHPGNIFVADDGRYIALDFGIVGTLDEYDKRYLAVNFLAFFNRDYH
                     RVATAHIESGWVPADTRAEELEAAVRTVCEPIFNRPLSQISFGLILMRLFETSRRFNV
                     EIQPQLVLLQKTLLNIEGLGRQLDPNLDLWATAKPFLVSWMNEQMGWKALKRNLEKEM
                     PDWANILPSLPRKLSDAISNSNKKYDIGLQKKIHNCQILITMIFIILVLIWFKL"
     gene            complement(3118..3678)
                     /locus_tag="PK856_02935"
     CDS             complement(3118..3678)
                     /locus_tag="PK856_02935"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HRL01359.1"
                     /translation="MWISHALINHLLLQQPELKQALSKLTGRSLRLTALGVNTDMHIR
                     EDGYVEAISAEQTFECHISIHASTWSKMVQGEAFGVGDVSITGDTDLAMQLLPVLGQL
                     RYEPYVDATRLFGNTVASHLDHQLKSLWRNTQQMVSRVEGEVRDYLQESDTPLVSQAQ
                     WQQRNDDIDALRDDVARLQARLQRLE"
     gene            complement(3689..4429)
                     /locus_tag="PK856_02940"
     CDS             complement(3689..4429)
                     /locus_tag="PK856_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRL01360.1"
                     /translation="MEKSVNRVWIAGAGYLGVPLVQQLQADDYAVSASSRTPREGVVW
                     HAWDFAAHSALQDIQAANTWVVLLPPSSGSDYVAHMRHLCAQAQQLHTAHVVYASSTS
                     VYGSEARVCDEESCVAPESDHAKQIVICEQIWQNSGLPCVSILRLGGLYDDERHPVRR
                     LSGRVAIQGGKHPVNVVSRSGAVAALAHHVAHAQGGIFNVVETAHPSRSEFYSQEALR
                     LSLPLPEFDASDESDGKTVISRHQCEHI"
     gene            complement(4414..5001)
                     /locus_tag="PK856_02945"
     CDS             complement(4414..5001)
                     /locus_tag="PK856_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRL01361.1"
                     /translation="MQKIAVYCGSNFGESKAYFDAAQELGRLLAQEQIGLVYGGGKVG
                     LMGTVADAVLAAGGQVTGVIPTFLREMEKAHVGLTELIETPDMTVRKQKMIDLADGYI
                     AMPGGVGTFEEWFEVLSMAQLRLQQKPIGLLDVNGFYQPLLRLIEQTITAGFMPESNL
                     SLFVVDSTPQGLLEKVRAYAPVYTQKWHDPAWKKV"
     gene            complement(5001..6077)
                     /gene="pheA"
                     /locus_tag="PK856_02950"
     CDS             complement(5001..6077)
                     /gene="pheA"
                     /locus_tag="PK856_02950"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019389377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HRL01362.1"
                     /translation="MSTDDLLAPHREAIDHIDQTILSLLNERAAHARAIGELKGSGVV
                     YRPEREAQVLRRIQSLNQGLLSNEAVARLFREIMSECLAVERPLTITYLGPEGTFTQQ
                     AAIKHFGHAAHTQPCATIDEAFRLVEARQADYVVAPIENSTEGAVGRTLDLLVASPLK
                     ACGEVVLRIHHHLLRKVAHQDGITKVYAHAQALSQCHEWLNKNLPANVARIAVASNAR
                     AAQLAAEDENAVAIASQAAAERFSLCKLASNIEDEPNNTTRFLVLGHQGTTPSGLDKT
                     SLIVSAPNKVGTVHELLQSLSKNGVSMTKLESRPSKAGLWEYVFFIDVEGHAEDEPVH
                     RALNDLQEEAAFLKVIGAYPKAVL"
     gene            complement(6184..6603)
                     /locus_tag="PK856_02955"
     CDS             complement(6184..6603)
                     /locus_tag="PK856_02955"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HRL01363.1"
                     /translation="MKALWMVLASLGLAACASTGTGSAEGNRSLAGSWRLTEVATQNV
                     PMTGGSAKLLQFEAATNKVSGNVGCNRLFGVYQSEGERLSFNGIATTRMACDPISMQM
                     ETNVVQNMEKVQTWSINGSELQLKDQAGQTILKFKRL"
     gene            complement(6627..>7512)
                     /gene="nagZ"
                     /locus_tag="PK856_02960"
     CDS             complement(6627..>7512)
                     /gene="nagZ"
                     /locus_tag="PK856_02960"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003791061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HRL01364.1"
                     /translation="HEGGRVQRFIQGFTRLPAMAVLKDVWQAQGKNAALTQAEEIGWV
                     LAAELRACGIDLSFTPVLDLDWDQCAVIGNRSFSADAQEVSDLALALQSGLKRGGMNS
                     CGKHFPGHGFVEGDSHHVLPQDTRGFDTLWQNDIVPFVHMSAAGMGAVMPAHVVYPQV
                     DDQPAGFSLFWLQDILREKIGFDGIIFSDDLTMEGACGVGGITVRTELSLQAGCDIAL
                     ICNRPDLVDELLSEWTWDVSTQPKLAERWERVAGQGLQADFEALLETAAFQQAQQRVA
                     ELATPKDIQNGVKVGEAF"
BASE COUNT         2119 a         1936 c         1495 g         1932 t
ORIGIN      
        1 ttttaaaaca attagtaaga gtgaatttag cctagttatc taggacagcc cctaatatta
       61 ttaagaagaa tgaatttcgt aagcctctta ccttaaacca ttaataatac aataatttct
      121 gcaaccaaag agcaactttt aggagagctt actcattcat gttgcctaac cactcactac
      181 ccccatattg ccaccaatat ggagctacaa ttgaatcatg acttccagaa gattggtaaa
      241 gttcactagt accgttccaa atcaaaaaat tagagtgttg ataaaataat aataatccca
      301 ccaaaacaat taccaaccca acaatatatt ttcgttgctt ttcccaaaat aaaggttttt
      361 ctatttgtgg taataaactt actacatatg caaaataaat catattaaaa atttgcgagt
      421 gcggataaac catagctcct gataacatcg cattcacact tatagaaata ataccagcaa
      481 cagaaagggg ggggagaaca caactctgtt tcaatattgc aagcacaaat acaattgaaa
      541 ttattgccaa atatatacct aatacaccat actctgtcca ccactgtaag aaaatattat
      601 gtggatgaaa aacaaaagta taattaattt tatcaaaact accaaatagc caattggact
      661 gccctaattg ccaagcttgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tgacctctac
      721 ctgaatcatc tattctttgt aaagccgtaa gtgctggttg attttgcaaa aaggtaaaca
      781 ctaatgctaa aagtactgca ctgatagtac caattataaa tatcctactc caacgataac
      841 ttctgtacca tattgcagcg aatatcaaac caaatatcaa tgagaaaatc actgctcttg
      901 aaccgttttg aatacttcca ctcaaaataa gcacccaaag agcccatcga aaaatctcaa
      961 agttttttgg tacttttaat aatgccaaaa taaaaagaaa cgggagaatc gcatcatcat
     1021 actgtctttt attaagaaaa ttacctagcc atgctttaga agcgatttct tctgggaaca
     1081 tatacatcaa gatactttca atcggaagcc acattacagt taaaagcggc atgcaactta
     1141 aaattatcaa atagaattct ttacttctgt ttatttgaaa tacaaatgaa taaataaaaa
     1201 ttgcaaaata tagtaatgaa tagactaata aaaaacttat ggaattaatt tgcttaggaa
     1261 taaataaaat ataaataata actattataa aaaggattac taaaggccca attaaccgta
     1321 ctataacaac gttacagatc cagatctctg gttttaatgc aatacaaaca attattacca
     1381 acaaagaatt tgctaaaagt ctccattcat catatgggaa atgtccccat ccaatgttat
     1441 acccactaaa aacaaaaaag gtataaacta acgaacaaat taatacaata tgagagaata
     1501 aatttttcat tccatgtctc tattgataaa gcttattcat atgccattaa ctcaagttat
     1561 gtataaacta caatttaaac caaatcaaaa ctaatataat aaagatcata gtaattaaga
     1621 tttggcaatt atgaattttt ttttgcaatc caatatcata ttttttattt gaattactaa
     1681 tcgcatctga taactttctc ggcaaactcg gcaaaatatt tgcccaatct ggcatttctt
     1741 tttccagatt gcgctttaag gctttccagc ccatttgttc gttcatccaa ctcaccaaaa
     1801 atggttttgc tgttgcccat aagtctaaat tggggtcgag ttggcggcct aggccttcga
     1861 tgttgagcag ggttttttgt aataagacca attgcggttg gatttcgacg ttgaagcgac
     1921 ggctggtttc aaacaagcgc atcaagatta agccgaatga aatttgcgat aaagggcgat
     1981 tgaaaattgg ctcacacacg gtgcgaacgg cggcttccaa ttcttctgca cgggtatcag
     2041 ctggcaccca accagattca atgtgcgctg tggccacacg gtgataatcg cggttgaaaa
     2101 atgccaagaa attcacggcc aaatagcgtt tgtcgtattc atccaaagtg ccgacaatgc
     2161 cgaaatccaa agcaatgtag cggccatcat cggccacaaa aatatttcct gggtgcatgt
     2221 cagcatggaa gaagccgttt ttgaacactt gggtaaagaa aatttccacc ccaaagcgtg
     2281 ccaatttttt caaatcaatg cccttggcaa ccaaggtgtc aacgtcagaa atcggcgtgc
     2341 catccatcca ctccatggtc atgacgctgc ggctgcaaaa atcaaaatac actttgggaa
     2401 cgatgagcat ggggctgttt tcaaattggc gccccaattg gctggcattg gccgcttcgt
     2461 gcatcaaatc caattcatcg tgcaggtatt tatcaaactc ttgcaccact tcgcgcggtt
     2521 tcaagcgttt gccatcgcgg aacaactttt ccaaccaaac cgccccccaa tgcatcaagg
     2581 ccaaatcctg ttcaatcaca ggcttgatgt caggtcgcaa tactttgacc gctacttttt
     2641 tgccttgtgt accatcttcg ttgaacaaaa aggcttggtg tacttgcgca atggaagcgc
     2701 tggcaatcgg ctcgcgttca aaagcgtaat aaatctgctc gattggtttg ccaaaagctt
     2761 tttcgatttg ggcaatggca atgccgccat cgaacgcagg cactcggtct tgcaattttg
     2821 ccaattcaaa ggcgtattca ggccctacga catcgggtcg ggtcgataaa acctgtccca
     2881 atttcacaaa tatcgggccc aagttttcca aggccaaacg caagcgcacg gcattgctgt
     2941 attccaaggt ttcttttgat tcgggcaaac aactgagcag cttacgcaac aaaggatggc
     3001 gcaactggcc atccaacaag ttaatcaaac caaaatacca aacggtatgc actatcaaac
     3061 gaaatcgttt taaccacttc atgctttaat ccaatgttta caggccggcc tgtaaggtta
     3121 ttcaagtctt tgcaaacgtg cttgcaagcg cgccacatca tcacgcaagg catctatgtc
     3181 gtcattgcgc tgctgccatt gcgcttgcga caccaaaggc gtatcgcttt cttgcaaata
     3241 gtcgcgcact tccccttcta cccgactcac catttgctgg gtattgcgcc acagcgattt
     3301 caattggtgg tctaaatggc tggcaacggt gttgccgaac aaacgggtgg catccacata
     3361 tggctcgtaa cgcaattgcc ccaacacggg cagcaattgc attgccaaat cggtgtcccc
     3421 cgtgatgctg acatcgccca caccaaacgc ttcaccctgc accattttgc tccaagtgct
     3481 ggcatggatg ctgatatggc attcaaacgt ttgctctgcg gaaatggcct caacatagcc
     3541 atcttcacga atgtgcatgt cggtattgac gcccaatgcg gtcaaacgca aagagcggcc
     3601 tgtaagcttg ctcaatgcct gttttaattc aggttgttgc aacagcaaat gattgatgag
     3661 tgcatgactg atccacatca tttttccttt aaatatgctc acattgatgg cgactgatga
     3721 ctgttttgcc atcgctttca tcgcttgcat caaattcggg tagcggtaag ctcaagcgca
     3781 atgcttcttg cgagtaaaac tcagaccgac tggggtgcgc agtctccacc acattgaaaa
     3841 taccaccttg cgcatgtgcg acatgatgtg ccaatgcggc aacagcacct gaacgtgaca
     3901 ccacattcac ggggtgtttg ccgccttgaa tggcaacacg cccgctcaag cggcgcacgg
     3961 gatggcgttc atcatcatac aggccgccta ggcgcaagat gctgacacaa ggcaagccac
     4021 tgttttgcca tatttgttcg cagatgacaa tttgcttggc gtggtcagac tctggcgcca
     4081 cacagctctc ttcgtcgcac actctcgctt ctgaaccata caccgaagtg ctagaagcat
     4141 acacaacatg ggctgtgtgc aattgctgtg cttgcgcaca caaatggcgc atgtgcgcca
     4201 catagtcgct gccgcttgaa ggtggcagca acaccaccca agtgtttgca gcttgaatgt
     4261 cttgcagcgc actgtgggca gcaaagtccc aagcatgcca caccacaccc tcacgtggtg
     4321 tgcgcgaact ggcgctgacg gcataatcat cggcctgtaa ttgctgcacc agcggcacac
     4381 ccaaatagcc agcgcctgca atccacacgc gatttacact tttttccatg ctggatcgtg
     4441 ccatttttgc gtataaacag gagcgtaagc acgcactttt tccaacaaac cctgtggcgt
     4501 gctgtctacc acaaacaaac tcaaattact ctcgggcatg aagcctgctg tgatggtttg
     4561 ctcaatcaag cgcaacaaag gctgataaaa accattcaca tccaacagtc ctatgggctt
     4621 ttgttgcaag cgcaattgcg ccatagacaa cacctcaaac cactcttcaa acgtgcccac
     4681 cccacctggc atggcaatat agccatcggc aaggtctatc attttttgct tgcgcaccgt
     4741 catgtcaggc gtttcaatca actcagtcaa gccaacatgg gctttttcca tttcacgcaa
     4801 aaatgtcggt atcacacctg tgacttgccc acccgccgcc aacaccgcat cggcgaccgt
     4861 gcccatcaag ccgactttgc cgccaccata caccaaaccg atttgctctt gcgccaacaa
     4921 ccgtcccaac tcttgggccg catcaaaata cgctttggat tcaccaaagt tagaaccaca
     4981 atacactgct attttttgca tcacaaaacc gccttagggt atgcgccaat gactttcaaa
     5041 aatgccgcct cttcttgcaa atcattcaaa gcgcggtgaa caggctcatc ttcagcatgg
     5101 ccttccacat caataaaaaa cacatattcc cacaagcctg ctttggatgg acgactctct
     5161 agcttggtca tggacacgcc atttttagac aaagattgca gcaactcatg taccgtgccc
     5221 actttatttg gcgcagacac tatcaatgag gttttgtcca agccgctagg cgttgtgcct
     5281 tggtggccca ataccaagaa acgggtggtg ttgttcggct cgtcttcaat attcgaagcc
     5341 aatttgcaca aactaaaacg ctcagccgcc gcttgtgagg ctatcgcgac cgcgttttca
     5401 tcttctgctg ccaattgcgc cgcacgggca ttgctggcca cagcaattct cgccacattg
     5461 gcaggcagat ttttgttcag ccattcatgg cattgtgaca aggcttgtgc atgggcatag
     5521 actttggtga tgccatcttg gtgcgctact ttgcgcagca aatggtggtg tatgcgcaat
     5581 accacttcac cgcaagcctt caaaggtgat gccaccaaca aatccaaagt gcggcccact
     5641 gcgccctcgg tcgagttttc aatcggtgcc accacataat cggcttgacg tgcctccacc
     5701 aaacggaacg cctcgtctat cgtggcacaa ggttgggtgt gggcggcatg accaaaatgt
     5761 ttgatggcgg cttgttgggt aaacgtgccc tcaggcccca aataagtgat ggtcaatggg
     5821 cgctctaccg ccaagcattc actcatgatt tcgcggaaca aacgcgccac cgcttcatta
     5881 gagagcaaac cttggtttaa ggattgaata cggcgcaata cctgtgcttc acgctcggga
     5941 cggtaaacca caccgctgcc ttttaattca cctatggcac gggcatgcgc agcgcgctca
     6001 ttgagcaagc ttaaaatggt ctgatcaatg tggtctatcg cttcgcgatg cggcgctaac
     6061 aagtcgtccg tactcatggg catcatccgt aataaaagcg ctcattttaa cccatattcc
     6121 aagccaaaag cggcctgtaa ggttttacag gccgctttta catgtattct agcaagttta
     6181 tgcttagagg cgtttaaatt ttaaaatggt ttgtcctgct tggtctttca attgcaactc
     6241 actgccattt atcgaccaag tctgcacctt ttccatgttt tgcaccacat tggtttccat
     6301 ttgcatggaa atcggatcgc aagccatgcg cgtggtggcg atgccattga aactcaagcg
     6361 ctcgccttcg ctttggtaca cgccaaacaa gcggttacaa cccacattgc cgctgacttt
     6421 gttggtagcc gcttcaaatt gcaataattt ggccgaaccg cctgtcatcg gtacgttttg
     6481 tgtagccact tcggtcaagc gccagcttcc tgccaatgag cgattgccct cagcagaccc
     6541 tgtacccgtg cttgcgcacg ctgccaaacc caaacttgcc aataccatcc acaatgcttt
     6601 catgctatcc ctttccttgc ttttcgttaa aacgcttcgc ctactttaac cccgttttga
     6661 atgtctttgg gtgtggccaa ttctgccacg cgttgttgcg cttgttgaaa cgcagcggtt
     6721 tccaacaagg cctcaaaatc ggcctgtaaa ccttggcctg ccacacgctc ccaacgctcg
     6781 gccaatttcg gctgcgtgct tacatcccac gtccattcag acaacaattc atccaccaaa
     6841 tcaggacggt tgcaaatcaa ggcaatatca caaccggcct gtaaagacag ctcagtgcgc
     6901 actgtgatac cgcccacgcc gcaagcgcct tccatggtca aatcatctga gaagatgatg
     6961 ccatcaaagc cgattttctc acgcaaaatg tcttgcaacc aaaaaagcga aaaacccgca
     7021 ggttgatcat ctacttgtgg gtataccaca tgcgcaggca tgaccgcgcc catgcctgcg
     7081 gcactcatgt gcacaaaagg cacgatgtcg ttttgccaca aggtatcaaa gccacgcgtg
     7141 tcttgtggca atacatggtg gctgtcaccc tcaacaaaac cgtgcccagg gaaatgcttg
     7201 ccacaactgt tcatgccacc acgtttcagg ccgctttgca aagccaaagc caaatcgctc
     7261 acctcttgcg catcggcaga gaaactgcga ttgccgatga ccgcacactg gtcccaatcc
     7321 aaatccagca caggggtaaa cgacaaatca atgccacacg cgcgcaattc tgccgccaaa
     7381 acccaaccaa tttcttcggc ttgcgtcaaa gccgcgtttt tgccttgtgc ttgccacaca
     7441 tctttcaata ctgccattgc aggcaaacgg gtaaagcctt gaatgaaacg ctgcacacgg
     7501 ccgccttcat gg
//
LOCUS       DAOMVK010000051         7473 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_2599_length_7473_cov_2.703963, whole genome shotgun sequence.
ACCESSION   DAOMVK010000051 DAOMVK010000000
VERSION     DAOMVK010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 7473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7473
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2599_length_7473_cov_2.703963"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PK856_02965"
     CDS             <1..546
                     /locus_tag="PK856_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HRL01365.1"
                     /translation="DQKAVKAAAETFRSNPDVDVVTTIAELGTGEALVSFLDEKGMPT
                     PVERAWILPPSSLLSPLQADVLTNKVQSDDLYATYKDAVDAYSAYEAILDFEKQQTAT
                     AEATEAATVTKTEATSAKEAEDPGLLDGLLTGLFGGRRKNNANISNKIGAQVGHTISN
                     KLTKSISDSITRTILGVIKGK"
     gene            612..2708
                     /gene="pta"
                     /locus_tag="PK856_02970"
     CDS             612..2708
                     /gene="pta"
                     /locus_tag="PK856_02970"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009118882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HRL01366.1"
                     /translation="MSAFIIVPTSNRAGLTSVSLGVLRAFEQSNIKPLFFKPIEQSYV
                     TQLEQQIDPAVHFAQELFHMPTVAPLALNEVEMLISQGKDDDMIELVVQRMSELRLQQ
                     AHDVVVIEGLAPDSDRPFLADKNLALARALQAEIILVVSALDVTAEQVAEQVSLYVHD
                     YDDDHVRIAGFVLNSINVPETEFAPFVEEVSKRIAANRAPLACLGAIQYAAEKSMFRM
                     KDLARYLNARVLHGSEYLDGSRVKELVVAGRSATHMVERFKAGAVIIASGDREDVMMA
                     TALRVISGTPLAGLILTCNEVPSPNLQALIAPALKADVPVLLTEHDTFNTANILSHMP
                     NGVPADDLCRMGNMVDYVAENLQINALIQNLDQPKDVRLSPPAFRYRMMQLARTANKR
                     IVLPEGTEPRTVQAAIICQQKGIARCVLLAKPEEVKDVAEKLNVALPLDLEILDPDLL
                     RQKYVEPMVKLRKHKGLTPELALRQLEDTVVLGTMMLAQNDVDGLVSGAIHTTANTIR
                     PALQLIKTKPDASLVSSVFFMLMPDQVLVYGDCAVNPEPSASELADIAIQSANSAQAF
                     GVEPKVAMISYSTGTSGMGAEVEKVKEATDLAKQKRPDLLIDGPLQYDAASVLSVGRQ
                     KAPDSPVAGQATVFVFPDLNTGNTTYKAVQRSANVLSVGPMLQGLNKPVNDLSRGALV
                     DDIVYTIALTAIQATQ"
     assembly_gap    3153..3162
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3399..3851)
                     /locus_tag="PK856_02975"
     CDS             complement(3399..3851)
                     /locus_tag="PK856_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01367.1"
                     /translation="MTDTTVFERVIWRLHADKLMLIHDNNVHTAMLGFQMVANWFNQS
                     PPSSLDIEYAIQSIEDGLAPLQAKLPAKAMYYSDDAMLVDLYKRLGISETRLTREQLE
                     ADFELVVQNLNRPMAMASMDLNKSAIAYELILREAMHHLDIAVLQRGI"
     gene            complement(3844..5289)
                     /locus_tag="PK856_02980"
     CDS             complement(3844..5289)
                     /locus_tag="PK856_02980"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRL01368.1"
                     /translation="MVRRQSGLNQSLQFRLALTISVVLLILAASVSVWSFQRTYNRVQ
                     TMQDRQMHALVNMVQDDNVSLVEDDAAQVSTASGPVSKPDAASSHTKPLPTPPTATPG
                     RIAPPHEQQTIIDTIDNPDLALLFDYPNVTAHLASLPDGFNDLQGKTDTWRAYLLTQK
                     DQRVIVAQPNGFRNRLARNNAWETLIPLLVLLPLLILVVYVTVYRVFKPIRSLTEEIA
                     SVEQLHAPSLAKHNIPTELRPFTQAVDDLFTRLNEAMQLNRRLIANAAHQLRTPLTAL
                     SLQAEQLQQHDLSPEELTQRLAQLQAGLARQRDLVLKLLALARSEEVLTQQQPVNLHI
                     AEEVGMVLQQCLPLALEKNIDLGVTELMEVKLALSSLDFQTLLQNLIENAVRYTPEGG
                     QVDVAITQLPQHIQISVTDNGIGIDDAHKDLVWDAFYRVDDNRHNSSGLGLAIVKNLV
                     DRYHGSIVLSDHQPQGLVVTVTFPISEHGHD"
     gene            complement(5265..5927)
                     /locus_tag="PK856_02985"
     CDS             complement(5265..5927)
                     /locus_tag="PK856_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003682209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRL01369.1"
                     /translation="MRILLVEDDLMIGQMVYEGLQQQAYAVDWVKDGEAASLALAAHD
                     YDVGLFDIGLPKKNGLQVLAELRSKRATLPVLLLTARDTVDDRIIGLDAGADDYLVKP
                     FEFKELLARIRAISRRSQGQADPILDNGVLKLNPATKEVSRGEQVVRLSSREFSLLLA
                     LMQHPGTIMSRSDLEERIYGWNEEVESNAIEFLIHSLRKKLGSDCIKNVRGLGWFVDK
                     AV"
     gene            complement(6019..7299)
                     /gene="efeB"
                     /locus_tag="PK856_02990"
     CDS             complement(6019..7299)
                     /gene="efeB"
                     /locus_tag="PK856_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003772433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HRL01370.1"
                     /translation="MTDKEDLQAASVANPSRRCLFKTAAVGAAGLAVGAAGGYLVSQQ
                     SNTANAGHSTQSIDCHGAHQAGILNPAHQPCGIFTAFDITGKTRGELQKLFRIITDRI
                     EFLTKGGKYPDAHPQLPPAGSGIIGINVPPDELTITVSLGSSVFDERFGLAALKPKHL
                     QPMMRFPNDMLRAEYCHGDFSLQFCADNHETNMHALRDIMKALTGLAVVRWSIDGFLP
                     KGEPGAAPRNLLGFKDGSANPKPKQADDFLWTGLSANSQGEPAWAKGGTYQAVRLIRN
                     LVEFWDRTPLKEQEMIFGRDKAHGAPLGMKGEYDEPDYSKDTDGKKIPMDAHMRLANP
                     RTKDTQKSLMLRRPYNYSLGLQKSGQLDVGLVFICYQANLQDGFIDVQKRLDGEPLEE
                     YIKPFGGGFFFTLPGFKKGEFVGQSLLMAAEQLA"
BASE COUNT         1839 a         1781 c         1951 g         1892 t
ORIGIN      
        1 gatcaaaaag cggtaaaagc cgcggctgaa accttccgca gcaatcccga tgtggatgtg
       61 gtcaccacca tcgccgaatt gggcacaggc gaagccttgg tttccttttt ggatgagaaa
      121 ggcatgccaa caccagtcga acgggcgtgg atattgccac cgagcagcct gttatcgcct
      181 ttacaggccg atgttttgac caataaagtt caatccgatg atttgtatgc cacttacaaa
      241 gatgccgtgg atgcatattc tgcgtacgaa gccattttgg attttgaaaa gcaacaaact
      301 gcaacggctg aagccactga agccgcaact gtcactaaaa ccgaagccac atctgcaaaa
      361 gaagcggaag acccaggatt gttagatggt ctgctcacag gtctgtttgg cggacgtcga
      421 aagaacaatg ccaatataag caataagata ggggcgcaag ttgggcacac catttccaat
      481 aagctcacca agtccatcag cgacagcatc acccgcacca ttttaggggt gataaaaggg
      541 aagtaatcgg gtaaggtaaa cggctttcag gcaactgaaa gcctttttgt ttgttaacga
      601 gcggaatcca tatgtcagca ttcatcatcg taccaaccag caaccgcgcg ggcttgacca
      661 gtgtcagctt gggcgttttg cgtgcttttg aacaaagcaa tatcaaaccc ttgtttttca
      721 aacccataga acaaagttat gtgacgcagt tggagcaaca aattgaccct gcggtacatt
      781 ttgcacaaga gctgtttcat atgcccacag tggcaccatt ggctttgaat gaagttgaga
      841 tgctgatttc tcaaggcaaa gacgatgaca tgattgaatt ggtggtgcag cgcatgagtg
      901 aattgcgttt gcaacaagcc catgacgtgg tggtgattga aggcttggct ccagacagtg
      961 ataggccttt cttggccgat aagaatttgg cattggcccg tgctttacag gctgagatta
     1021 ttttggtggt cagcgctttg gatgtcactg ctgaacaagt ggcagaacaa gtgagtttgt
     1081 atgtgcacga ctacgatgac gaccatgtgc gcatcgctgg ctttgtgctg aattccatta
     1141 atgtccccga aaccgagttt gccccttttg ttgaagaagt ctcaaaacgg atcgcggcga
     1201 atcgcgcacc attggcctgt ttgggcgcaa tacaatatgc ggcagaaaag tccatgttcc
     1261 gcatgaaaga cttggcccgc tatctgaatg cccgtgtgct gcatggctcc gagtatttgg
     1321 atggctcgcg tgtgaaagaa ttggtcgtgg cagggcgttc ggccacacac atggtggagc
     1381 gctttaaagc aggcgcggtg atcattgctt cgggtgatcg agaagatgtg atgatggcca
     1441 ctgccttgcg cgtcatcagt ggcacgcctt tggcgggttt gatactgacc tgtaacgaag
     1501 tgccgtcccc caatttgcag gccttgattg cccctgcctt gaaagccgat gtgcctgttt
     1561 tgttaacaga gcacgatact ttcaataccg ccaatatttt gtcgcacatg cccaatggtg
     1621 ttcctgcgga tgacttgtgc cgcatgggca atatggtgga ttatgtggcg gagaatctgc
     1681 aaatcaatgc cttgattcaa aacctagatc agcccaaaga tgtgcgtttg tcgcctcctg
     1741 ctttccgcta tcgcatgatg caattggcgc gcaccgcgaa taagcgcatc gtcttacctg
     1801 aaggcaccga gccacgtacg gtacaggccg ccattatttg tcaacaaaaa ggcattgccc
     1861 gctgtgtgtt gttggccaag cctgaagaag tcaaagacgt ggctgaaaaa ctcaatgtgg
     1921 ccttgccttt ggatttggaa attcttgacc ctgatttgct gcgccaaaaa tacgttgagc
     1981 ccatggtgaa attgcgcaag cacaagggtt tgacgcccga gttggccttg cgccaattgg
     2041 aagacacggt ggtgttgggc acgatgatgt tagcgcaaaa cgatgtcgat ggcttggtgt
     2101 caggtgccat acacaccacc gccaatacca tacgccccgc cttgcaattg attaaaacca
     2161 aacctgatgc cagcttggtt tccagcgtat ttttcatgtt aatgccagat caagtgttgg
     2221 tttatggcga ttgcgcggtc aacccagaac cgtcggcttc ggaattggca gacatcgcca
     2281 ttcaatcggc gaattcagcc caagcatttg gcgtggagcc caaagttgcg atgatttcat
     2341 attccacggg cacgtctggc atgggtgctg aggttgaaaa agtgaaggaa gccaccgatt
     2401 tggccaaaca aaaacggcct gatttgctga tagatgggcc tttgcaatac gatgccgcca
     2461 gtgtgttgag cgtgggtcgt cagaaagcgc ccgacagccc tgtggcaggt caagccacgg
     2521 tgtttgtgtt ccccgatttg aacacaggca ataccactta caaagccgta cagcgtagcg
     2581 ccaatgtctt gagcgtggga cctatgttgc aaggcctgaa caagcctgtg aacgatttgt
     2641 cgcgtggtgc tttggttgac gacattgtct acacgattgc tttaaccgcc atacaggcga
     2701 cgcaataagt cagatggttt gaagcgttga taaataaatg cggcctgtaa acttacaggc
     2761 cgcatttgtc attgatatga aggatatgaa gcagttgaac aagagtatgg ttttaatctc
     2821 gtcacacaag cgtgggattt ggtacatgag gtaagtaagc gcagctgtac caggcttgag
     2881 gtgaataagc gtggttgcgc aacgcattgt aggaagcacg atacaaaacc taaatatgaa
     2941 aaagcggcct gtaaatgttt acaggccgct tttgatgcat ggtcattcat aatcatcttg
     3001 ccatgaagac gaattcacag ggccgtagcg gccttgtcca tttttatgaa tgcgcaattt
     3061 tttgctacga taaattttgc tgccctcggc acgctgttcc tcagatgctt ggggacggtc
     3121 tttctttttt tgagacttgc cctgctcagg gtnnnnnnnn nntaagctga tgaggctgaa
     3181 acacacacac agcaacagtg gtaatttcat ggtgtactcc aaaagaggct ggtttgcggc
     3241 agcaggcaga ttgtctgtca gggaaatccg cctgctgatg ggttcgcaaa accgatatga
     3301 acatgtgttg gatgcttcgt tcatatggct tgcagtatag gcaagcagac ttagtacaaa
     3361 cttagaagta tgttaaatag ggttaaaatc aaaatctttt aaatgccgcg ttgtaacacc
     3421 gcaatgtcca aatggtgcat ggcttcgcgc aaaatcaatt cgtaggctat ggccgatttg
     3481 ttcaaatcca tgcttgccat ggccatgggc cgattcaggt tttgtaccac caattcaaaa
     3541 tcggcttcca attgttcgcg ggtcaatcgg gtttcgctga tgcccagccg tttgtacaaa
     3601 tccaccaaca tggcatcgtc actgtaatac atggcctttg cgggcaattt cgcttgcagc
     3661 ggcgccaaac cgtcttctat ggattgtatg gcgtactcaa tgtccaatga ggatggtgga
     3721 ctttggttaa accaattggc caccatttga aaacccagca tggcggtatg cacattgttg
     3781 tcgtggatca acatcaattt atcggcatgt aaacgccaaa tcacgcgctc aaacacagta
     3841 gtatcagtca tggccatgct cacttattgg aaaagtcacg gtaaccacca agccttgcgg
     3901 ctgatggtcg ctcaaaacaa tactgccatg gtagcggtct accaaatttt tgacaattgc
     3961 caagcccaaa cccgagctgt tgtgacggtt gtcatcgacg cgatagaacg catcccaaac
     4021 caagtctttg tgggcatcgt ctatgcctat gccattgtcg gtgacgctga tttgtatgtg
     4081 ttgtggcaat tgggtaatgg ccacatcgac ttggccacct tcgggtgtgt agcgcaccgc
     4141 attttcaatc aggttttgca ataaggtttg aaaatccaaa ctgctcaaag ccaatttcac
     4201 ttccatcaat tcggtcacac ccaaatcaat gtttttttcc aaggccaaag gtaggcattg
     4261 ttgcaacacc atgcccactt cttcggcaat gtgtaagttg acaggctgtt gctgggttaa
     4321 tacttcctca ctgcgtgcca aagcaagcag cttcaacacc aaatcgcgtt ggcgtgccaa
     4381 accggcctgt aattgagcca aacgctgtgt cagctcttca gggcttaaat cgtgttgttg
     4441 taattgctcg gcctgtaaac tcaaggccgt gagaggggtg cgcaattggt gggcggcatt
     4501 ggcgattaaa cgccgattta actgcatggc ttcattcaaa cgtgtgaata agtcatctac
     4561 tgcttgggtg aaggggcgca gttctgtggg tatgttgtgc ttagccaacg atggggcgtg
     4621 caattgttcc acgctggcga tttcttcggt gaggctgcgt atgggtttga acacacggta
     4681 aaccgtgacg tacaccacca aaatcaacaa aggtaacaag accaataagg gaattaaggt
     4741 ttcccatgcg ttgttgcgcg ccagccgatt gcgaaagcca ttcggttgcg ccacgatgac
     4801 gcgttggtct ttttgggtaa gcagataggc tcgccaagta tcggttttgc cttgtaaatc
     4861 attgaaacca tcgggtaggc tggccaagtg tgctgtgaca ttgggatagt caaacagcaa
     4921 ggccaaatcg ggattgtcta tggtgtcaat gatggtttgc tgctcatgcg ggggggcaat
     4981 gcgaccaggc gtggcagtgg gtggtgtggg caggggtttg gtgtggctgg atgcagcatc
     5041 aggtttgctt acagggccag aggcagtgct cacctgagca gcatcgtctt ctaccaagct
     5101 gacattgtcg tcttgcacca tattgaccaa ggcatgcatt tgcctgtctt gcatggtttg
     5161 gactcggtta taggtgcgtt ggaacgacca cacgctgacc gaggccgcca aaatcagcaa
     5221 aaccacgctg atggtcaagg ccaaacgaaa ctgtaaagat tgatttaaac cgctttgtcg
     5281 acgaaccatc ccaagcctct gacgttttta atgcagtcgc tgcccaactt cttgcgcaag
     5341 ctgtggatta aaaattcaat cgcattgctt tccacttctt cgttccagcc ataaatgcgc
     5401 tcttccaaat cgctgcgcga catgatggtg ccaggatgtt gcatcagcgc gagcagcagg
     5461 gaaaattcgc gcgaggacaa acgcaccact tgctcgcctc ggctgacttc tttggtggct
     5521 gggttgagtt tcaacacgcc attgtcgagt atcgggtcgg cttggccttg gctgcgacgg
     5581 ctgatggcac ggatgcgagc aagcaattct ttgaattcaa atggtttgac caaatagtcg
     5641 tcagctcccg catccaaacc gatgatgcgg tcgtctacgg tgtcgcgtgc ggtcaacagc
     5701 aaaacaggca aggtggcgcg tttgctccgt aattcggcca agacttgcaa gccatttttc
     5761 ttgggcaagc caatatcaaa caagcccaca tcgtagtcat gggcagccag agccaaactg
     5821 gcggcctcgc catctttgac ccaatcaacc gcgtaagctt gctgttgtaa gccttcatag
     5881 accatttggc ctatcatcaa atcgtcttca accagtaata tgcgcatgcc aatttctttg
     5941 tgaggatagg ggtgaattat gacacagcca cacctcaaaa cggggtgtgg ctgtgtgaat
     6001 acaggtaagg aagcgaggtt acgccagttg ttcagctgcc atcaataagg attggccaac
     6061 aaactcgcct tttttgaagc ctggcaaggt gaagaagaag ccaccgccaa aaggcttgat
     6121 gtattcctct aaaggctcgc catccaagcg cttttgcaca tcaataaaac cgtcctgtaa
     6181 attggcttgg tagcaaataa acaccaaacc cacgtccaat tgccctgatt tttgcaagcc
     6241 caaagagtaa ttgtagggac ggcgcaacat caggcttttt tgggtgtctt tggtgcgcgg
     6301 attggccaag cgcatgtggg catccatggg gattttttta ccatctgtgt ctttgctgta
     6361 atcaggctcg tcgtattcgc ctttcatgcc caatggcgca ccatgggctt tgtcacgacc
     6421 gaaaatcatt tcctgttctt tcagcggcgt gcgatcccaa aattcaacca agttgcgaat
     6481 taagcgcacg gcttggtaag ttccaccttt ggcccaagca ggttcgcctt ggctgttggc
     6541 cgacaggccc gtccacaaaa agtcatcggc ttgtttgggc ttcgggttgg ctgagccatc
     6601 tttaaagccc agtaaattgc gcggtgcggc gcctggttcg cctttgggca aaaagccatc
     6661 aatgctccaa cgcaccactg ccaagcctgt taaggctttc atgatgtcgc gcagcgcgtg
     6721 catattggtt tcgtggttat cagcgcaaaa ctgcaaggag aaatcgccat ggcagtattc
     6781 agccctgagc atgtcattgg gaaagcgcat catcggttgt aagtgtttgg gttttagcgc
     6841 agccaaacca aagcgttcgt caaacacact actgcccaaa gacacggtga tggtgagctc
     6901 atcaggggga acattgatcc caatgatgcc gctgcctgct ggtgggagct gggggtgggc
     6961 atcgggatat ttgccacctt tggtcaaaaa ctcaatgcgg tcggtgatga tgcgaaacag
     7021 tttttgcaat tcaccgcggg ttttgcccgt gatgtcaaac gcagtgaaga tgccgcaagg
     7081 ctgatgagca gggtttaaaa tccctgcttg gtgcgcaccg tggcagtcta tggactgggt
     7141 tgaatggcct gcattggcgg tgttgctttg ctggctgacc aaatagccac ctgctgcgcc
     7201 cacagccaaa ccagcagcgc ccacagcagc ggttttaaac aagcaacgac ggctgggatt
     7261 ggccacagat gcggcctgta aatcttcttt atcggtcatg tgaccacttc ctttacaaaa
     7321 ggctgcctaa aaggcagccc gtgtcttcat agcgctaagc cgcttagtct aagcccaaag
     7381 tgccgcgcaa tttagccaat tcttccgcca aggtttggac aggtgctttc aaagcggtgc
     7441 ggtcggcgtc gctcaaagca tcataggttt taa
//
LOCUS       DAOMVK010000052         7445 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_2618_length_7445_cov_3.085386, whole genome shotgun sequence.
ACCESSION   DAOMVK010000052 DAOMVK010000000
VERSION     DAOMVK010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 7445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7445
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2618_length_7445_cov_3.085386"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1343
                     /locus_tag="PK856_02995"
     CDS             <1..1343
                     /locus_tag="PK856_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009116856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRL01371.1"
                     /translation="NGTEKEWHYPNGLESYLRDLIEQNNAEMAVPIFHTANYIGKKHE
                     DFQAGEGAAFAITFLEDGHINNESYVNLIPTPLGGTHVAGLKQAMFTAVSNFIEHHSL
                     LPRGVKLQSDDVFAKSAFVLSARVLDPQFQGQTKDKLTNRDALRLVSTVSVDAMELAL
                     NQDTEIGKRIAELAIKQAQARQRSVKKIEKRKGSGVAVLPGKLTDCESEDIRENELFL
                     VEGDSAGGSAKLARDKATQAILPLRGKVLNSFETHPDQLFANNEIHDISVAIGVDPHQ
                     KGDKVDLSGLRYGKIAILSDADVDGAHIQVLLLTLFYRHFPELIAHGHIYVSQPPLFR
                     VDVNAIGKSKPARKIYALDQQELDSILERLVQEGVKESAYSISRFKGLGEMNPDQLKD
                     TTMHPDTRRLMQVSIPEDGHDDTLAIFVKLMGKGEAASRRAWMEAEGNHVEVDV"
     gene            1413..2543
                     /gene="nemA"
                     /locus_tag="PK856_03000"
     CDS             1413..2543
                     /gene="nemA"
                     /locus_tag="PK856_03000"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HRL01372.1"
                     /translation="MKAYPFLFQNILIGSMNVPNRVIMAPLTRLRSSLPGDIPTDLMA
                     EYYTQRASAGFIITEATQVSAQAKGYQGAPGLHSDAQQHAWAKVTQAVHAKGGRIGVQ
                     LWHTGLISHQSLQPNLQAPISASATQDLLSRTSLQDSAGQIYRVVTTPSRAASSDDIQ
                     QVIADFAAATLRARAAGFDFVEIHGAHGYLLHQFLVAALNQRNDAYGGSRENRARLLL
                     DVVDACIAAWDAEHIAIRISPIGKFNGMPGDFGAEDTLWLVDELNKRGLMYLHISEPD
                     YAGAASLSDAYRVKIRRRFKQAIIGAGLYTPAKANRLIEAGLIDAAAFGRDFIANPDL
                     PERFATRAELNLYRGELAYGGGAAGYTDYPFLETQCEGATHD"
     gene            2536..3420
                     /locus_tag="PK856_03005"
     CDS             2536..3420
                     /locus_tag="PK856_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HRL01373.1"
                     /translation="MTETTASLLPYLGVLKVSGEDRAPFLHNQLSNDISNLPASHACY
                     ATYSTPKGRVLANMLVLNTGEELWLIMSADLIDAIAKRLRMFVMRSKAVLENVSDRLG
                     VASSLIPTDEDYALPAEPPLLLNAEFRNGVWHIALPHAGVLCVGELTALPVHNEKAEL
                     QWRSHEINHGYPWVRAANSETCVAQMLNQHLLGGVHFRKGCYPGQEIIARAQYRGQVK
                     RGLVMYGSNQLVEPGEKLFDDAGEEVGVVINAIPQAMGSLILAVVKYAAADESLNTEK
                     AATLDFKRRFYTVAEEAA"
     gene            3417..3911
                     /locus_tag="PK856_03010"
     CDS             3417..3911
                     /locus_tag="PK856_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HRL01374.1"
                     /translation="MIGALKAWAFAKPTRMRRFMNVWPPFLFAGIYVQELSEDYTFCR
                     VKLRAWVATRNVNGSQFGGNLFAMTDPIYPLMLLGIFGKDYFVWDKEATIEFIKPAFH
                     AAYFECRLSLAEIAAIKQACADGDKHFPVLENHIVDAQGNILAKVKRTLYIRLKPDHR
                     PQQS"
     gene            4143..4565
                     /locus_tag="PK856_03015"
     CDS             4143..4565
                     /locus_tag="PK856_03015"
                     /inference="COORDINATES: protein motif:HMM:NF024922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HRL01375.1"
                     /translation="MQARLTASFGKAYNYSQMTYPEQTMLAVLADLLPIIETEVGFLP
                     NNCLINHYANGQARMGYHSDQTDILLEDTGIVILSLGSERNLVFRNTDEPEQKISYTL
                     AHGSLLYMNQAVQQLWQHAIPTSKAKDIRLSLTFRCLQ"
     gene            complement(4643..>4988)
                     /locus_tag="PK856_03020"
     CDS             complement(4643..>4988)
                     /locus_tag="PK856_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01376.1"
                     /translation="MEAFQGVFVATTNLMKHLDQAALRRFDFKIEFGFLRLEQRWSLF
                     QAYCQHFGIECPAALEYNVGKLHMLTPGDFAVVAKCARITPIASAQHLLDLLQAECAL
                     KENGSKGNFGFL"
     assembly_gap    4989..5021
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5022..6683)
                     /locus_tag="PK856_03025"
     CDS             complement(<5022..6683)
                     /locus_tag="PK856_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRL01377.1"
                     /translation="MSAIETPSILQQNQIDPKTADLLRAWVWQMMLPLGGQSDFIGEH
                     GFDDESVANLLQATQWFSDADAYEPVAARAHMKKHFLKWQKTIQWDEVYQHGVPEVLQ
                     QNIQAWQQLLGLNAVEAKILTFVILMYQTNVLSQAAQFMGELNSRQVVLCLSVVLQLT
                     EAEVRLALGHEQALFHTGLLKIDHSSEYPLRSKIDLMSSQFAEWMCQEVISPVELLKR
                     HVHTAPTTELSLQDFNHLGTLVPALQHYLPKVVAKQHKGCNILVYGVAGTGKTEFTRA
                     LSQDLGLECIEVAWADDEGNPASREARMSALRAAQHIFAKQNMLLLFDEVEDVFNDGQ
                     KDYQLNKGWLNRMLEQNAVPTVWITNRVDVMDRAFLRRFDMIIEMKAPPRAKRREMIA
                     RLTHGFLTEAEIEQLAEHEDLVPAMLERAHKVTEEVGDVLPEREQAALYKQLLHNTLK
                     AQGISSHLNRKADIGAVYDIQWINSRQNLQQLADGFAQSQVGSLCLYGPPGTGKSAYV
                     KWLAKQMDKPLLYKKGSDLLSKYVGESEQLIAAAFEEAARDNAVLI"
     gene            complement(6796..>7445)
                     /locus_tag="PK856_03030"
     CDS             complement(6796..>7445)
                     /locus_tag="PK856_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HRL01378.1"
                     /translation="HSNYRMIRAIKNRFGAANELGVFAMTDKGLKGVANPSAIFLASY
                     RDDVPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLTVGLEQNRLAMLLAVLNRHA
                     GIACFDQDVFLNAVGGVKIMEPAADLAVILAMLSSYRNKPLPEKMIAFGEIGLSGEVR
                     PVSRGQERLKEAEKLGFTHAIVPQANLPRHQQDFPQLTLYGVTSLQEAVDICRRD"
BASE COUNT         1875 a         1990 c         1737 g         1810 t
ORIGIN      
        1 ccaatggcac tgaaaaagaa tggcattacc ccaatggctt agaaagctat ttgcgcgatt
       61 tgatagagca aaacaatgcc gaaatggccg tgcccatatt ccacaccgcc aattacattg
      121 gtaagaaaca cgaagacttt caagcaggcg aaggcgcggc ttttgccatc acctttttag
      181 aagatggtca catcaacaat gaaagctacg tcaacctgat tcccacgcct ttgggcggca
      241 cacatgtagc cggcctgaaa caagcgatgt ttactgctgt gagcaatttc attgaacacc
      301 acagcttatt gccacgcgga gtgaaactgc aatcggatga cgtgtttgcc aaatcggctt
      361 ttgtcttgtc tgcgcgcgtg ctcgacccac aatttcaagg ccaaaccaaa gacaaactga
      421 ccaaccgcga cgccctgcgt ttggtgtcta ccgtttcagt cgatgcaatg gagttggcgt
      481 tgaaccaaga cactgaaatc ggcaaacgca ttgccgaatt ggcgattaag caagcacaag
      541 cgcgccaacg ttcggtgaaa aaaattgaga aacgcaaagg ctcaggcgtg gcagtattac
      601 ctggcaaact caccgattgc gaaagcgaag acatccgcga aaacgaattg tttttggtag
      661 agggtgactc tgcgggtggc tcagccaaac tcgcgcgcga caaagccacc caagcgattc
      721 tgcctttgcg cggcaaggta ttaaactcgt ttgaaaccca tcccgaccaa ttgtttgcca
      781 acaatgaaat ccacgatatt tccgtggcca taggagtaga tccacatcaa aaaggtgaca
      841 aagtcgattt atcgggtttg cgctatggca aaatcgcgat tttgtccgat gccgatgtag
      901 acggtgcgca catacaggta ttgttgctca ctctgttcta tcgccatttc cctgaattga
      961 ttgcccatgg ccatatttat gtgtcgcaac caccgttgtt ccgagtggat gtcaatgcca
     1021 taggcaaaag caaacctgca cgtaaaatct atgcgctaga tcagcaagag ttggacagca
     1081 ttctcgaacg cttggtacaa gaaggtgtga aagaaagtgc ttacagcatc agccgtttca
     1141 agggtttggg tgaaatgaat cctgaccagc tcaaagacac caccatgcat cccgacaccc
     1201 gtcgcttgat gcaagtgtct attccagaag atggccacga tgacactttg gccatttttg
     1261 tgaaattgat gggcaaaggc gaagccgcca gccgccgtgc atggatggaa gccgaaggca
     1321 accatgtcga ggtggatgtg taatccaaac ccttatggca agctgctcac ttgtttacag
     1381 gccgcatctt gcaccacaca gcaaggaacc acatgaaggc ttaccctttt ttgtttcaaa
     1441 acattctcat cggtagcatg aacgtgccca atcgtgtcat catggcgccg ctaacacgct
     1501 tgcgttcaag tttaccaggt gacattccca ccgatttgat ggccgagtat tacacccagc
     1561 gcgccagcgc aggcttcatc attaccgaag ccacccaagt gtctgctcaa gccaagggct
     1621 accaaggcgc acctggtttg cacagcgatg cccaacagca tgcatgggca aaagtcactc
     1681 aagcagtaca tgccaaaggt ggccgcattg gagtgcaatt gtggcacact ggcttgattt
     1741 cacaccaaag cctgcaaccc aacttacaag ccccgatttc tgcctcagcc acacaagatt
     1801 tactctcacg taccagtttg caagacagcg caggtcaaat ctaccgcgtt gtcaccacgc
     1861 catcgcgcgc cgccagcagt gatgacattc agcaagtgat tgcggatttt gctgccgcta
     1921 ccctccgcgc gcgcgccgca gggtttgatt ttgtcgaaat ccacggtgcc catggctatt
     1981 tattgcacca atttttggtg gccgctctca atcagcgcaa cgatgcctat ggtggcagcc
     2041 gcgaaaaccg tgcacgcttg ttattggatg tggtcgacgc ctgcatcgct gcttgggatg
     2101 ctgagcacat agccatacgc atttcgccga taggcaaatt caacggcatg ccaggcgatt
     2161 ttggcgcaga agacacgctg tggttggttg acgaactcaa caagcgcggc ttaatgtatt
     2221 tacacatctc tgagcccgat tatgcaggcg cagcctcttt aagcgatgct tatcgggtta
     2281 aaatccgtcg ccgtttcaaa caagccatca ttggagccgg cctgtacacc ccagccaagg
     2341 ccaatcgcct gattgaagca ggcctgattg atgccgccgc atttggccgc gatttcattg
     2401 ccaatccaga tttgcctgaa cgctttgcca cccgtgccga actcaacctc taccgaggtg
     2461 agctcgctta cggtggcggc gcagcaggct ataccgatta cccattttta gaaacacaat
     2521 gcgaaggagc cacacatgac tgaaaccacc gccagtttgt taccgtattt gggcgtgtta
     2581 aaagtcagcg gtgaagaccg cgccccattc ttgcacaacc aattgtccaa cgacatcagt
     2641 aatctgcccg cttcccatgc ctgttacgcc acctacagca cacccaaagg ccgtgtgttg
     2701 gcgaatatgt tggtgctcaa cacgggtgaa gagttgtggc tcatcatgtc cgcagatttg
     2761 attgatgcca tcgccaagcg cttgcgcatg tttgtgatgc gcagcaaagc ggtgttggag
     2821 aatgtcagcg accgcttggg tgtggccagc agcctgattc ccaccgatga agactatgcc
     2881 ttgcctgccg agccaccgtt gttgctcaat gccgaattcc gcaatggggt ttggcatatt
     2941 gccctgcccc acgcaggtgt gttgtgtgtg ggcgaactta ctgctctgcc agtgcacaat
     3001 gaaaaagccg agttgcaatg gcgcagccac gaaatcaacc atggctatcc atgggtacgt
     3061 gcggccaaca gcgaaacctg tgtcgcccaa atgctgaacc aacacttact cggtggcgta
     3121 cactttcgca aaggctgcta cccaggccaa gaaatcattg cccgcgcgca ataccgtggc
     3181 caagtcaaac gcggcttggt catgtatggc tccaatcaat tggtggagcc gggtgaaaaa
     3241 ctgtttgatg atgcgggcga agaagtgggc gtggtcatca acgccatacc acaagccatg
     3301 ggttcattga ttttggccgt ggtcaaatat gccgccgcag atgagagcct gaacactgaa
     3361 aaagccgcta ccttagactt caaacgccgc ttctacactg tggcagaaga agccgcatga
     3421 taggcgcgtt aaaagcttgg gcttttgcca aaccgacgcg catgcgtcgg tttatgaatg
     3481 tgtggccgcc gtttttgttt gcgggcattt atgtgcaaga attgtcggaa gactacactt
     3541 tttgccgtgt gaaactgcgc gcctgggtcg ccacacgtaa tgtcaacggc agccagtttg
     3601 gcggcaattt gttcgccatg accgacccca tctacccttt gatgttattg ggcatttttg
     3661 gcaaagacta ttttgtctgg gacaaagaag ccaccatcga attcatcaaa cctgcttttc
     3721 acgccgctta ttttgagtgt cgtctgagcc ttgcagaaat cgcggccatc aagcaagcat
     3781 gcgccgatgg cgacaagcac tttcctgtat tggaaaacca cattgtcgat gcccaaggca
     3841 acattcttgc caaggtcaaa cgcactttgt acatccgttt gaaaccagac caccgacccc
     3901 aacagtctta atgctgtgtt aaaaacattt acaggccgct tatctgcatg aaaagcggtc
     3961 tgtaactatt atgttctaac catgtcatgg ttgtgctata gatttaagca ttcaattcat
     4021 tgcccactct acccatgaat ccccatacac ttatcaaagg cgtttgctat ctacccaatt
     4081 ttattgctca tgcagacaac ttactgactc atttattaca agaagtcgtg tgggatactc
     4141 gcatgcaagc acgcttaacc gcgagttttg gcaaggctta caattactcg caaatgacgt
     4201 atcctgaaca aaccatgttg gctgtattgg cagatttact gccaatcatt gaaacagagg
     4261 ttggttttct gcccaataat tgtttgatta atcattatgc caatggtcag gctcgcatgg
     4321 gttatcactc cgaccagaca gacatattgc tcgaagatac aggcattgtg attctttctc
     4381 tgggctcaga gcgcaacctc gtttttcgta atacagacga gccagaacaa aaaatctcct
     4441 atactcttgc ccatggcagc ttgctctaca tgaaccaagc cgtgcaacag ctttggcaac
     4501 atgccattcc gacaagcaaa gccaaagaca ttcgcctgag cttaacattt cgttgcttac
     4561 aataaatgcc aagacggtta caggccgctt atgtcttaca caagcggcct gtaatctgta
     4621 actacacttc ttttatatca agctatagaa agccaaagtt gcctttagaa ccattttctt
     4681 tgagtgcaca ttcggcctgt aacaaatcca ataaatgctg agcagaagca atgggtgtga
     4741 tgcgtgcgca tttggccacc acagcaaaat caccaggcgt gagcatgtgc agcttgccca
     4801 cgttgtattc caatgctgca gggcactcaa taccaaaatg ttggcaatag gcttggaaca
     4861 agctccaacg ctgctccaaa cgcaggaagc caaattcaat tttaaaatca aaacggcgca
     4921 gcgccgcttg gtccaaatgc ttcatcaaat tggtggtggc gacaaacacg ccttgaaaag
     4981 cctccattnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naatcagcac ggcattgtcg
     5041 cgcgcggctt cttcaaatgc ggccgcaatc aattgctcgc tctcacccac gtatttcgac
     5101 agcaaatctg agcctttttt gtacagcaat ggcttgtcca tttgcttagc caaccatttt
     5161 acatacgcac tcttgcctgt gcctggcggg ccatataaac acaagctgcc cacttgcgac
     5221 tgagcaaaac catcggccaa ttgttgcaga ttttggcggc tgttaatcca ttgaatgtcg
     5281 tataccgcgc caatgtccgc cttgcgattg aggtgggaac tgatgccttg cgccttgagt
     5341 gtgttgtgca gcaattgctt gtacaatgcc gcctgctcac gctctggcaa cacatcgccc
     5401 acctcttctg tgactttgtg ggcacgctct aacatcgcag gcaccaagtc ttcgtgttcg
     5461 gccaattgtt cgatttcggc ttcggttaaa aagccgtggg tcaaacgggc aatcatttcg
     5521 cggcgcttgg cacgtggcgg cgctttcatt tcgatgatca tgtcgaagcg gcgcaagaac
     5581 gccctgtcca tcacgtcgac tcggttggta atccacaccg taggcacggc attttgctcc
     5641 aacatgcgat tgagccaacc cttgttcaat tggtagtctt tttgcccatc gttgaacaca
     5701 tcttccactt catcaaacaa cagcaacatg ttttgcttgg caaaaatgtg ttgcgccgca
     5761 cgcaaagcac tcatgcgtgc ctcacgactg gcaggattgc cttcatcatc agcccaagcc
     5821 acttcgatgc attccaaacc caagtcttgc gacaatgcac gcgtgaactc ggttttgcct
     5881 gtgcccgcca cgccgtatac caaaatatta cagcccttgt gctgtttggc caccactttg
     5941 ggcaaataat gttgcaacgc aggtaccaaa gtacccaaat ggttgaagtc ttgtaaactc
     6001 agttcagtgg tcggagcggt atgcacatgg cgcttgagca actcgacagg gctgatgacc
     6061 tcttggcaca tccactcggc aaattgactg ctcatcaaat cgattttact gcgcagcggg
     6121 tattcgcttg aatggtcgat tttcaataag cctgtgtgaa acaaggcttg ctcgtgtccc
     6181 aaagccaagc gcacttccgc ctcagtcaat tgcaacacca ccgacaagca caacaccact
     6241 tgacggctgt taagctcgcc catgaactgc gccgcttgcg agagcacatt ggtttggtac
     6301 atcaaaatga caaaagtcag gatttttgct tcaaccgcat tcaagcccaa caattgctgc
     6361 catgcttgta tgttttgttg caatacctca ggcacgccat gttggtagac ttcgtcccac
     6421 tgtatggttt tttgccattt caaaaagtgt tttttcatgt gcgcgcgtgc cgctactggc
     6481 tcgtacgcat cggcatcact gaaccactgc gtggcttgca acaaattggc caccgattca
     6541 tcatcgaagc catgttcacc gatgaaatcg gactgccctc ccaaaggcag catcatttgc
     6601 catacccatg cccgcagcaa gtctgctgtt ttggggtcga tttgattttg ctgcaagata
     6661 gatggggtct cgattgcgct cattgtttca ttcattgtat taatcaaaaa tcatgatggt
     6721 gcgaaaatat caggcatccg tttgaagcgg cctgtaaacc gctgtaacct catatggtga
     6781 caatggcgcc gactttcaat cccgacgaca aatgtccaca gcctcttgta atgaggtcac
     6841 accatacagg gttaattgtg gaaagtcttg ttgatggcgt ggcaaattgg cctgagggac
     6901 gatggcatgg gtaaagccca gcttctccgc ctctttcaaa cgctcttgcc cacgtgatac
     6961 aggccgcacc tcacccgaca accctatttc accaaaagca atcatttttt caggcagcgg
     7021 tttgttgcga tagctcgaga gcatggccaa aatcaccgcc aaatcggcag ctggctccat
     7081 gattttgaca ccgcccacgg cattgagaaa cacatcttgg tcaaaacagg caatgcccgc
     7141 gtggcgattg agcacggcca ataacatggc caagcgattt tgctccaaac ccacggtcaa
     7201 acgcttgggt gtaaaaccat gtgcatcatc caccaaagct tggatttcca ccaataaagg
     7261 ccgtgagcct tcttgcgtca ccaacacaca agagcctggc acatcatcgc ggtatgaggc
     7321 caagaaaatg gccgatggat tggccacgcc tttcaagcct ttgtctgtca ttgcaaacac
     7381 acccagctcg ttggcggcac caaagcggtt tttaatcgcg cgtatcatgc ggtaattgct
     7441 gtggc
//
LOCUS       DAOMVK010000053         7358 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_2661_length_7358_cov_2.881966, whole genome shotgun sequence.
ACCESSION   DAOMVK010000053 DAOMVK010000000
VERSION     DAOMVK010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 7358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7358
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2661_length_7358_cov_2.881966"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PK856_03035"
     CDS             <1..556
                     /locus_tag="PK856_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="Bcr/CflA family drug resistance efflux
                     transporter"
                     /protein_id="HRL01379.1"
                     /translation="GFCFSCMFVFLTESTWVYKNYYGLSNKQYALAFMFNIVAMASFN
                     RLTAYRLKKGAQPHKILLSGLAVQFTTGLILAIWCLIALPPFPVMVFLVMTTIGAQGL
                     LVPSNQAIYMSYFSREAGSANAVLGAGMQMIAFGIGWLTTEMHHITDGKITVMPLMMF
                     ASTLSAIAAIGLLSRNAFKAQRSQ"
     gene            616..1620
                     /locus_tag="PK856_03040"
     CDS             616..1620
                     /locus_tag="PK856_03040"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HRL01380.1"
                     /translation="MTFATGSRRNIAVLYTGGTIGMQAGEYGLRPSKESVLEALAAYQ
                     QDIDWHVQVCEPLIDSSLVAPHHWLAWQQWIEAQQCDGVLILHGTDTMAYTANVLAFA
                     LGDIAMPVVITGAQHPLGTAGGDAESNLNGAVQALLQMAAPKAVSLVFDGKIMAAVGS
                     RKISTRSLDGFSNKNQAPLAQWQHGHWQGLQAACDVSKPRLPIPLNPDVCIASHYCLP
                     VNEQWALRVLLQSGQADAVIVQSFGHGNGGFSEADVTAMQEFQQQGGTIINISQVADG
                     HVAAAYEASKSLRDAGVFMAGSWTVETAYAKALVGLSAGWRGDALREYLQLNVLGEWA
                     "
     gene            complement(1729..2220)
                     /locus_tag="PK856_03045"
     CDS             complement(1729..2220)
                     /locus_tag="PK856_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003683615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRL01381.1"
                     /translation="MSLPTILRPAKASDCDDIYQAHLYAVRYTCSSTYNETILTAWSA
                     LLSPESYLETMALPNKTLWVIEYKGHVQGFFQLDTAEAQLDALYVHPFVFKQGLGTAL
                     LQRAEKIAFEAGLSFIKLYASLNSVPFYELNDYSTLGDAALHLNSEVTVECQLMRKYL
                     QVD"
     gene            complement(2233..2838)
                     /locus_tag="PK856_03050"
     CDS             complement(2233..2838)
                     /locus_tag="PK856_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01382.1"
                     /translation="MKHILLLGCLSALMVPLAQANVYSCVVNGEKIYTNKKVGNCQSE
                     KLDKIGSYTSDRSIIERQNRLSNEKIAAQNGGNQDSNNSAPPVQQASASPPSSRPVRP
                     IAAAAVPTRPATNVPAVANNNAPAAPAQSNRDQGRREILMSELTNEKKALAQARLALT
                     NGKLIRNQADARKQQSMTSLQRAVEDRQQNIQALQNELARM"
     gene            complement(2849..4777)
                     /locus_tag="PK856_03055"
     CDS             complement(2849..4777)
                     /locus_tag="PK856_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003683445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRL01383.1"
                     /translation="MIYIQNLTLQRGTKVLFEQANLTINPNQRVGLIGKNGTGKSTLF
                     SLFKGELHSDGGDVLVPQHWLIASVAQNTPALECSALDYVLQGDAPLQSLQAALKQAE
                     ADNNGEEMAHMLAQLDEIDAYSAPARAGKLLNGLGFSTEEQQRPVKTFSGGWRMRLNL
                     AQALMCRADLLLLDEPTNHLDLETVLWLEQHLANLNCTQIIISHDRDFLNAVTNTTVE
                     LSQQKLTVYGGNYDFYERERALRLAQQQSAYVKQQAHIAHLQSFIDRFKAKATKAVQA
                     QSRIKALARLELIAPAHLDSEFAFEFIEPAHLPNPLLKLDEVNLGYGDTTIVQNVSLS
                     IESGARIGLLGVNGSGKSTLIKTIAGEITPMSGQIIRAEKLAIGYFAQHQLETLRSDQ
                     SPIWHIQQLSPQVREQEIRNFLGGFQFQGEMAMQAIAPFSGGEKARLALAMIVWQQPN
                     LLLLDEPTNHLDLDMRHALTVALQSFQGALIVVSHDRSLLEATTDGFVFMHEQRLHQF
                     DGDLNDYRLWRLQQEQLANQTSSGSAPSSSQNRKDSKRLQAQLRQQLSQQTKPLQKKI
                     ASTEATMAKLGAEQQELEAFLGTEDAYLADNKAQMQQSVTRLGEIKTQLATLEEDWLM
                     WQDELEQLSASIQAEFGE"
     gene            complement(4774..5010)
                     /locus_tag="PK856_03060"
     CDS             complement(4774..5010)
                     /locus_tag="PK856_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01384.1"
                     /translation="MASYGQTPTLAHDHASLPSVIIPKKTQPFTQRKLQESYQVFHRE
                     SALIAYQINKRPVNAHSTAADGVKMAARFMCVTS"
     gene            5161..5661
                     /gene="tpx"
                     /locus_tag="PK856_03065"
     CDS             5161..5661
                     /gene="tpx"
                     /locus_tag="PK856_03065"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HRL01385.1"
                     /translation="MAQVTLKGNAVEVGGNFPQAGAAAPDFQLVNGSLQSQSLADFAG
                     KRKILNIFPSVDTGVCAQSVRHFNQDAAGLDNAVVLCISADLPFAQARFCGAEGIENV
                     VMLSAFRSPEFKQAYGVDMTSGPLQGLCARSVVVLDTDNKVIYSQLVPEITEEPDYAA
                     ALAALK"
     gene            complement(5738..>6633)
                     /gene="tsaD"
                     /locus_tag="PK856_03070"
     CDS             complement(5738..>6633)
                     /gene="tsaD"
                     /locus_tag="PK856_03070"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRL01386.1"
                     /translation="YGGVVPELASRDHIHRLVPLTRACLAEAQVDLADIDAIAYTQGP
                     GLGGALLTGTSFANALAFANDKPVIPVHHLEGHLLSPMLAEDKPEFPFVALLVSGGHT
                     QIMAVTGVGQYELLGESIDDAAGEAFDKTGKLLGLPYPGGAKLSQLAKLGLTGRFEFP
                     RPMLHSHDLNMSFSGLKTAVLTAVNKVKAEHGEVDEQTRNDICLGFQDAVVDVLSRKV
                     QDALKQTGFDSLVVAGGVGANWKLRERLNQLKARVFFPPLSLCTDNGAMIAFAGAMHL
                     QDAHEVGAFDVKPRWPLIATR"
     assembly_gap    6634..6676
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7034..>7358
                     /locus_tag="PK856_03075"
     CDS             7034..>7358
                     /locus_tag="PK856_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HRL01387.1"
                     /translation="MRNTLIASAVSMVLLAACGGGNTGAATEKKTEHASASNNAVAAS
                     ETKTPLAALPETKELKVYNWSDYVDPATIKDFEKNSGIKVTYDVYDSNESLEAKVLTG
                     KSGYDL"
BASE COUNT         1761 a         1708 c         1938 g         1908 t
ORIGIN      
        1 aggcttttgc ttcagctgca tgtttgtgtt tttaaccgaa tccacttggg tgtacaagaa
       61 ttattatggt ctgagcaaca aacaatacgc gttggcattc atgtttaaca tcgtcgccat
      121 ggcgagcttc aaccgcctta ctgcctatcg tctgaaaaaa ggtgcccagc cgcacaaaat
      181 tctgctcagt ggtttggctg tgcaattcac cacgggttta atcttggcga tttggtgttt
      241 gattgcctta ccaccgtttc cagtcatggt gtttttggtg atgaccacca ttggcgccca
      301 aggcttactc gtgccatcta atcaagccat ttacatgtct tacttcagcc gagaggcagg
      361 cagtgccaat gcggttttgg gtgctggcat gcaaatgatt gcttttggaa taggctggct
      421 aaccacggaa atgcaccaca tcaccgatgg caaaatcacc gtcatgccat tgatgatgtt
      481 tgcctctaca ttgagcgcga tagctgccat tggcttgttg tcacgcaatg cgtttaaggc
      541 acagcgcagt caataatatt ttcaaataca ctggatgact gtgatttaca ggccgctatt
      601 tgatggagaa atgccatgac tttcgcaaca ggctcacgtc gcaatatcgc tgttttgtac
      661 acggggggca ccataggcat gcaagcggga gagtatggat tacgccccag taaagagtcg
      721 gtattggagg ctttggctgc ctaccaacaa gacatagact ggcatgtgca agtgtgcgag
      781 cccttgattg attcatcatt ggtcgctcca caccattggt tggcatggca acaatggata
      841 gaggcgcaac agtgtgatgg ggtattgatt ctccatggca cagataccat ggcctatacc
      901 gcaaatgtgt tggcttttgc cttgggcgac atcgccatgc ccgtggtcat cacgggggca
      961 caacacccgt tgggcactgc aggaggagac gcggaaagca atttgaatgg tgcggtacag
     1021 gctttgctgc aaatggctgc accaaaagcc gtgagtctgg tgtttgatgg gaaaatcatg
     1081 gcagctgtgg gcagccgcaa aatcagcact cgcagcttgg acggattttc caataaaaac
     1141 caagcgccgt tggcgcaatg gcaacatgga cattggcaag gcttacaggc tgcttgtgat
     1201 gtgtccaaac cacgtttacc aataccgctg aatcctgatg tatgcatcgc cagccattat
     1261 tgtctgcccg ttaatgagca gtgggcattg agggtgttat tgcaatcggg gcaagccgat
     1321 gcggtgattg tgcaaagttt tgggcacggt aatggcgggt ttagtgaggc cgatgtgact
     1381 gccatgcaag aatttcagca gcaaggtggc accatcatca acatttctca agtggccgat
     1441 ggccatgtgg ccgcagcata cgaggcatct aagagcttgc gcgatgcggg ggtgttcatg
     1501 gcgggcagtt ggacagtgga aacggcttat gcaaaagccc tggttggctt gagtgcaggt
     1561 tggcgagggg atgcattgcg agaatatttg cagctcaatg ttctgggcga atgggcgtaa
     1621 gaaaaagcaa atggccatgt acacaacatg gccattatta aataccgaaa caaatatgct
     1681 gaagtctttt attgggtgaa taagcggcct gtaagctctg tttgtgattc aatccacttg
     1741 taagtatttg cgcatcaatt ggcattccac cgtgacttct gaattcaaat gcaaagcagc
     1801 atcacccagt gtggaatagt cattgagttc ataaaacggc acagaattga gtgaggcata
     1861 cagtttgatg aagctcaagc ctgcttcaaa agcaattttt tcggcccgtt gcagcaaggc
     1921 cgtgcccaag ccttgcttga acacaaatgg gtgcacatac aaggcatcca attgggcttc
     1981 ggccgtatcc aactggaaaa aaccttgtac atggcctttg tattcaatca cccacaaagt
     2041 cttgttgggc aaggccatgg tttccaaata gctctcaggc gagagtaagg cagaccaagc
     2101 ggtcaaaatg gtttcgttat acgtgcttga acaggtatag cgcacggcgt ataggtgcgc
     2161 ttggtaaatg tcatcacaat ccgaagcttt ggcagggcgc aaaatggtgg gcaaactcat
     2221 gcagcattca tcctacatgc gcgccaattc gttttgtaag gcttggatgt tttgttggcg
     2281 gtcttctact gcgcgttgca agctggtcat ggactgctgt ttgcgtgcat cggcctgatt
     2341 acggatgagc ttgccattgg tcaaagccaa acgggcttgc gccaaggctt ttttctcatt
     2401 ggtcaattcc gacatcaaaa tctcacggcg accttggtca cggtttgatt gtgctggcgc
     2461 agcaggagca ttgttgttgg caacggctgg gacattggtg gcaggacgag tgggcacagc
     2521 tgccgcagca attgggcgca ctgggcgcga actcggcggg cttgcagatg cctgttgtac
     2581 gggaggggca ctgttgttgc tgtcttgatt gccaccattt tgtgcggcaa ttttttcatt
     2641 gctcaaacgg ttttgacgct caatgatgct gcggtcactg gtgtaagaac cgattttatc
     2701 caatttttcc gattggcaat tgcccacttt cttattggtg taaatttttt cgccattgac
     2761 gacacagctg tacacatttg cttgtgccaa aggcaccatc agtgcgctta aacagcccag
     2821 aagtaagatg tgtttcatga tgattccctt attcgccaaa ttcggcttgt atgctggcag
     2881 acaattgttc caattcgtct tgccacatca gccaatcttc ttccaaagtg gcgagttgtg
     2941 ttttgatttc gcccaaacgc gtgacacttt gttgcatttg ggctttgtta tcagccaaat
     3001 aagcatcttc tgtacccaga aacgcttcta gctcttgttg ttctgcgccc aattttgcca
     3061 ttgtcgcctc ggtagaggca atttttttct gtaaaggctt ggtttgttgt gacaattgtt
     3121 gtcgcaattg cgcttgcaag cgcttggaat ctttgcgatt ttggctgctt gatggcgcgc
     3181 tgcctgagct ggtttggttg gccaattgct cttgctgcaa acgccacagt cggtaatcgt
     3241 tcaaatcacc atcaaattgg tgcaaacgct gctcgtgcat gaacacaaag ccatctgtgg
     3301 tggcttcgag caagctgcgg tcatgggaga ccactatcaa agcgccctga aagctttgta
     3361 aagccacggt caaggcatga cgcatgtcca aatccaagtg gttggtcggt tcgtccagca
     3421 gcaataaatt cggttgttgc catacgatca tcgccaaagc caaacgggct ttttcgccac
     3481 cagaaaacgg cgcaatcgct tgcatcgcca tctcgccttg gaattgaaaa ccacccaaga
     3541 aattgcggat ttcttgctcg cgcacttgtg ggctcaattg ctgtatgtgc caaatcggcg
     3601 attggtctga gcgcagggtt tccaattggt gttgggcgaa atagcctatg gccagttttt
     3661 ctgcacggat gatttgtcca gacattgggg taatttcgcc cgcaatggtt ttaatcaatg
     3721 ttgacttacc actgccgttc acgcccaaaa ggccgatgcg cgcgccgctt tcaatcgaca
     3781 ggctgacgtt ttggacgatg gtggtgtcgc catagcccaa gttcacctca tccagtttta
     3841 aaagggggtt gggcaagtgg gcgggctcga tgaattcaaa tgcaaactcg ctgtccagat
     3901 gcgcaggggc aatcaattcc aagcgcgcca aggctttgat gcggctttgc gcttgcaccg
     3961 ctttggtggc tttggctttg aagcggtcga taaacgactg caaatgggcg atgtgggctt
     4021 gttgcttcac ataagccgat tgctgctgcg ccaagcgcag ggcgcgttcg cgttcgtaaa
     4081 aatcataatt gccgccatac accgtgagct tttgctgtga caattccacc gtggtgttgg
     4141 tgacggcatt gagaaaatcg cggtcgtggg aaatgatgat ttgggtgcaa ttgaggttgg
     4201 ccaaatgctg ctctaaccac aacaccgttt ccaaatccaa gtggttggtc ggctcgtcca
     4261 atagcaacaa atcggcgcgg cacatcaagg cttgcgccag attcaaacgc atgcgccaac
     4321 cacctgaaaa ggtttttaca ggccgctgtt gctcttcggt agagaagccc aagccattga
     4381 gcaatttgcc tgcacgggca ggggcgctgt aagcatcgat ttcatccaat tgcgccaaca
     4441 tgtgtgccat ttcctcgcca ttgttgtcgg cttcagcttg ttttaaagcg gcctgtaagc
     4501 tttgcaatgg cgcatcgcct tgtaacacat aatctaaagc cgaacattcc aaggcgggcg
     4561 tgttttgcgc caccgatgca atcagccaat gttgcggcac caacacatcg ccgccatcgc
     4621 tgtgcaattc gcctttgaat aaggaaaata aagtggattt ccctgtgccg tttttgccta
     4681 tcaagcccac gcgctgattg gggttgatgg tgaggttggc ttgctcgaac aagactttgg
     4741 taccgcgttg cagggtcagg ttttgaatgt aaatcatgat gtcacacaca taaatcgagc
     4801 cgccatttta acaccatctg cagcagtgct gtgggcgttt acaggccgct tgttgatttg
     4861 ataggcaatc aatgccgatt ctctatgaaa cacttgataa gactcttgta acttgcgctg
     4921 ggtaaatggc tgtgttttct taggaatgat gacggatggt aaagaggcat ggtcatgagc
     4981 aagcgtagga gtctgtccat aggaagccat ttgcttggca atgttttaat ctgcgtaaag
     5041 agcgcttgtg ccttgcgaag ctcaggctat aatgccgtgg aagctgagaa cagcggcgct
     5101 gttcatgggc attcacattc aatacaacac catcatcagt cacacatcta ggagagcttt
     5161 atggcgcaag tcacattaaa aggcaatgca gttgaagtag gcggaaactt cccacaagct
     5221 ggcgctgctg cgcccgattt tcaattggtg aacggcagct tgcaaagcca aagcttggcc
     5281 gattttgcgg gcaaacgcaa aattttgaac attttcccaa gcgtggacac aggtgtgtgc
     5341 gcgcaatcgg tgcgccattt taaccaagat gcagcaggct tggacaatgc ggtggtgctg
     5401 tgtatctctg ctgacttgcc atttgcacaa gcgcgttttt gcggtgccga aggcatagaa
     5461 aacgtggtga tgttgtcggc attccgcagc cctgaattca aacaggcgta tggcgtggac
     5521 atgacttctg gcccattaca aggtttgtgc gcgcgttcgg tggtggtttt ggacacagac
     5581 aacaaagtga tttactcaca attggtacca gaaatcaccg aagagcctga ttatgcagcg
     5641 gctttggcag cattgaaata ataccctgtt tgcaatatag caagcggcct gtaaaccttg
     5701 aaatgggttt acaggccact tttacgtctg tttgaactta acgcgtggca atcaaaggcc
     5761 agcgcggttt gacatcgaaa gcgccgactt cgtgcgcgtc ttgcaaatgc atggcccctg
     5821 cgaaggctat catcgcgcca ttgtcggtac acagtgacaa cggagggaag aacacgcggg
     5881 ctttgagttg gtttaagcgc tcgcgcagtt tccagttggc acccacgcca cctgccacca
     5941 ccaatgaatc aaaacctgtt tgtttgagtg catcttgcac tttgcggctg agcacatcga
     6001 cgacggcatc ttgaaagccc aagcaaatgt cattgcgggt ttgctcatcg acttcgccgt
     6061 gttcggcttt gactttgttc acggcggtta agacggcggt ttttaaacct gaaaaactca
     6121 tgttcaaatc gtgtgaatgc agcatggggc gcgggaactc aaagcggcct gtaagcccta
     6181 gtttggccaa ttgcgatagc ttggcaccgc caggataggg caagcccaag agtttgcctg
     6241 ttttgtcgaa ggcttcacct gcggcatcgt ctatgctttc acccaacaat tcgtattggc
     6301 ccacgcctgt gaccgccatg atttgcgtgt gcccacccga caccaataag gcgacaaaag
     6361 gaaactctgg cttgtcttct gcgagcatgg gtgagagcaa gtggccttcc aaatggtgca
     6421 caggaatcac aggtttgtcg ttggcaaacg ccaaggcatt ggcaaaactg gtgcctgtga
     6481 gcagggcacc acccaagcct gggccttggg tgtaggcgat ggcatcaata tcggccaaat
     6541 ccacttgcgc ttcggcaaga cacgcacggg tgaggggcac gagtctgtga atgtggtcgc
     6601 ggctggccaa ttcgggcacc acgccaccgt attnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnaaac cgcgctcggt gtcatacagt gccacaccgg tttcatcaca
     6721 agacgactct atacccaaaa ccaacatttt gttttcctta ttgatagacg tgtattgtag
     6781 cttaatttgc acccatttgc cctgatttat gtgcagttgc agcattggcg gagagctgag
     6841 ctgatggctt aagtgagtgt gaatctcttt acaatttaac aggatagaaa tctgtgtaag
     6901 cctattgtca tatgtctcaa tagcaggttt aatgaaagtg caactggtgt gtttggccag
     6961 tttacgacaa ggctcttgcc catactcaaa acaatacttg gcaaggtttg agcagaaatc
     7021 aaaggatgac aatatgagaa acactttaat cgcatcagct gtcagcatgg ttttgttggc
     7081 ggcgtgtgga ggtggcaata ctggtgctgc cacagagaag aaaactgagc atgcgagcgc
     7141 cagcaacaat gcggttgccg ccagtgaaac caaaacaccg cttgcggcat tgccagaaac
     7201 caaagagctc aaagtttaca attggtcgga ttatgtagac ccagccacca tcaaggattt
     7261 tgaaaaaaac agtggcatta aagtgactta tgatgtgtat gacagcaatg agtctttgga
     7321 agccaaggtt ttaacgggca agtcgggtta tgatttgg
//
LOCUS       DAOMVK010000054         7334 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_2673_length_7334_cov_3.618629, whole genome shotgun sequence.
ACCESSION   DAOMVK010000054 DAOMVK010000000
VERSION     DAOMVK010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 7334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7334
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2673_length_7334_cov_3.618629"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..320
                     /locus_tag="PK856_03080"
     CDS             93..320
                     /locus_tag="PK856_03080"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRL01388.1"
                     /translation="MKKAASPKSFEEAVARLEAITQAMQGNELSLEDALAAYKEGNEL
                     VRFCQQKLADVEQQLQVLDGDELKELTLNDD"
     gene            310..1200
                     /locus_tag="PK856_03085"
     CDS             310..1200
                     /locus_tag="PK856_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRL01389.1"
                     /translation="MTIDFPAWLTATQAQAEAAVERYLPTTSTGQDTLHEAMSYVSVG
                     GGKRFRPLLVAAAARLGAAEAEALSQAMAAIEYVHVYSLVHDDMPEMDNDSLRRGKPT
                     CHVQYDQATALLVGDALQTLAFDVLSRPVNLPAAQQLQRVQVLARASGSEGMAGGQGI
                     DLANVGKDLSLAQLQHMHGLKTGALIRAAVALGGLSCSDVSDGLLADLDVYADKLGLA
                     FQVIDDVLDCEQDTATLGKTAGKDEEANKPTYVKLLGLEGARTQATSLAEEAKAALAT
                     YGDAAAALLGLADYVIKRNH"
     gene            1314..1793
                     /locus_tag="PK856_03090"
     CDS             1314..1793
                     /locus_tag="PK856_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01390.1"
                     /translation="MKIKHAIVACATALCFGTVVAAPTATYHGQWKSVGGETLIVNAS
                     GVKSYTEMRKECTPQYFKLSNTQHSGAKLQSTLQTTLKHNQQDLASSQSSDNSNPYPN
                     NIRNIQRILPLVNTKQTYSSITVMCMDGAKELIFINPQNAVEIIDGGGETLFELYRK"
     gene            1986..2453
                     /locus_tag="PK856_03095"
     CDS             1986..2453
                     /locus_tag="PK856_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01391.1"
                     /translation="MVDKLTLFLLASILSSLSLADTKPLHNSWRNDSGYTVPSNGVTT
                     PTCSLAPSQNSTHTNTYQGSELIERLQHSLKESEAIITDDLASTPTSEQTMYRNNIQA
                     IKEVLPLIQPNTRYTSQASLCGTRQRSWLVINKHLALEEHSATDVVTYVIFRR"
     gene            2545..3042
                     /locus_tag="PK856_03100"
     CDS             2545..3042
                     /locus_tag="PK856_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01392.1"
                     /translation="MTLKQTVLMAICLSATASSFAAINNNAATHGQWRNSDGNTISVG
                     ADGVKQYADNADECRSMGYKMTGARFKGSEIKASMQSTLAYDRENLPDLKADNRYAES
                     ADSLAVNIQTIQRLLPMVDAKKTYAGIAMQCGDGSSELIFLDNHNAIEQNFGGGETYY
                     EHYRK"
     gene            3236..4231
                     /locus_tag="PK856_03105"
     CDS             3236..4231
                     /locus_tag="PK856_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01393.1"
                     /translation="MVTAEDIFKLVFFIYWGAMLSVIGLVWLFAWKLVSKGNKWWVGL
                     LGTAAAAAIFVLPMFKQDVKTKQEDKELQVRQEKYLAAKAVFDERCKDAGYKIYKTVE
                     NVEGVTLLNVWQGDPRNEDQMWEYAGLPRASGEDGYIQGFLYWRAWDFQTNDYNYYGN
                     GFVLDSKYQHKNLPNEVKQRYKNLNSYKYVDVAQNSIYQRYQYKELYDPSEISITTVQ
                     KPSRYTVEFENPIIPEDRNIWLATTKAFVRDTKTGTLLGEATWYSLHGGQGVKKYSTT
                     GIWDRAKTCPEIASAQDGPIQYFVLKVLKPKQFPPQLQSKPEETNDVETLKPKAQ"
     gene            complement(4356..5153)
                     /locus_tag="PK856_03110"
     CDS             complement(4356..5153)
                     /locus_tag="PK856_03110"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRL01394.1"
                     /translation="MQERYSHGYDDSVLNSYRSRSAANSAAYLLPHLQADMRLLDVGC
                     GPGSISADFADVLAQGQVNAVDVSPESIAAAQAMYARDNLQFETASAYALPFADASFD
                     VVHAHQVLQHLAEPARALREMARVLKPNGILAVRDTAYNSMTWQPATPALQQWLDVYQ
                     AIATHSGGNPNIGPELQRLCEAVGFTDIVTSTSPWHYHAANGSAQWWGEMWSVRVLKS
                     DFSKLAQEQGLLTDASLQQLSHGWQTWATLPNAEFTMTHHQILARKA"
     gene            complement(5175..6176)
                     /locus_tag="PK856_03115"
     CDS             complement(5175..6176)
                     /locus_tag="PK856_03115"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRL01395.1"
                     /translation="MTSHTVLRYSLSALLGLSATSWAQTPTQLAPQQQIPGVYKAQIG
                     QTRVIALFDGVVALKRSELAQISPEQVQESLTGRYVPETKDGLQTAVNAFLIDTGREQ
                     VLVDTGTANCFPGDLGHVAENLRYAGYRTEDIDAVVLTHGHPDHMCGLLSTDGKPIYA
                     NAQVWLDKAELQYWQSPQNKRKAPKKFAFLFDLANKSLQPYLQSQRVHTFSAGTTLPA
                     GLQVLPTPGHTIGHSAFVLPSKNERDNLMLWGDVVHYHSVQLQHPEATYEIDQDYAQS
                     VASRRKVLQQAAARGWWVAGAHMPFPGLGHVRPQGQGYEWVASEFSPLPASAALPQP"
     gene            complement(6257..7096)
                     /locus_tag="PK856_03120"
     CDS             complement(6257..7096)
                     /locus_tag="PK856_03120"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRL01396.1"
                     /translation="MDVVTEDAPSQTVYALLAQLQTVANTCAVVASRHSHVVRAVDIL
                     QPQLGVVLQGRKQISCGDTQLNIHVGEILLSKRACSVNVANLTDDYGLCYATVCIPIS
                     ETVLQAARLLQGEAVLTMATEPAIACEKIDVCAQALSAWAGAVLAEDEVAARLALTQV
                     VLHLVAQGHSALLLPPSKDLAAQIYDLVRNRPAHAWQSAEIEQHLHLSGATLRRRLAA
                     QGSSLKQVILHARLNCALDMLYTSTWPLKTIAAKCGYQSVSVFNQRFLQRYGLLPVDI
                     RSE"
BASE COUNT         1929 a         1899 c         1779 g         1727 t
ORIGIN      
        1 cgagaaagaa aaattattgg ctttgcgtcc tgaaacctac atcggcaaag ccgttgaatt
       61 ggccaaacgc atttaagcga taaggacagt ctgtgaaaaa agccgcaagc ccaaaaagct
      121 ttgaagaagc cgttgcccgt ttagaagcca tcacccaagc catgcaaggc aatgagttga
      181 gcctcgaaga cgcgttggcg gcctacaaag aaggcaatga attggtgcgt ttttgtcagc
      241 aaaagcttgc cgatgtcgag cagcaattgc aggttttgga cggagatgaa ttgaaggagc
      301 ttaccctcaa tgacgattga tttccctgct tggctcacgg ccacgcaagc ccaagccgaa
      361 gccgccgttg aacgctattt gcccaccact tccacaggcc aagacacgct gcacgaagcc
      421 atgtcttacg tcagtgtggg cggtggcaag cgtttccgtc ctttgttggt ggctgcggcc
      481 gcgcgtttgg gcgctgccga agcagaagcc ttgagccaag ccatggccgc catcgagtat
      541 gtgcatgtgt attctttggt acacgacgac atgcccgaaa tggacaacga cagcctgcgc
      601 cgcggcaagc ccacttgcca tgtgcaatac gaccaagcca ccgccttatt ggtgggcgat
      661 gccttgcaaa ccttggcgtt tgatgtgttg agtcgacctg tcaacttgcc cgccgcgcag
      721 caattgcaac gcgtacaagt attggcccgc gcctcaggca gcgaaggcat ggcaggcggt
      781 caaggcatag acctggccaa tgtcggcaaa gacttgagcc tcgcgcaatt gcaacacatg
      841 cacggcctca aaacaggcgc cttaatccgc gccgccgtgg ctttgggtgg cctctcttgc
      901 agcgatgtgt cggatggctt gttggccgat ttggatgtgt atgccgacaa actcggcttg
      961 gccttccaag tcatagacga cgtgctcgat tgcgaacaag acaccgccac cttgggcaaa
     1021 accgctggca aagacgaaga agccaacaag cccacttatg tgaagctgtt gggtttagaa
     1081 ggcgcacgca cccaagccac aagcttggcc gaagaagcca aagccgccct ggcgacttat
     1141 ggcgacgccg ccgcagcctt gctcggtttg gccgattacg tgattaagcg caaccattaa
     1201 ttgctaccgt gagtattctc aagcggcctg taagatacag gccgcttttt cttttggatt
     1261 gaattctgta tgctgatttt ctgtttactt ggctagccta aaaggaaacc aacatgaaga
     1321 tcaaacacgc gattgtcgct tgcgctacgg ctctgtgttt cggtacggta gtcgccgctc
     1381 ccactgccac ctatcatggt caatggaaaa gtgtcggtgg tgaaacattg atagtgaatg
     1441 cttctggggt taaatcgtat acagagatgc ggaaagaatg cactcctcaa tattttaaac
     1501 tctcaaacac ccaacattca ggtgccaaat tacaatccac actccaaaca acgcttaagc
     1561 acaatcaaca agacctcgcc tcctcccaat cttcagataa ctccaatcct taccccaaca
     1621 atattcgcaa tattcaacgc atattgccgt tggtcaatac caaacaaacc tacagcagca
     1681 tcacagtaat gtgcatggat ggcgcaaaag aactgatttt catcaaccca caaaatgcag
     1741 tagaaatcat tgatggtggt ggcgagacac tgtttgagtt gtatcgaaaa taatgtcgtc
     1801 agcaatgctg atcgtgtttg agctaacttg cataagaaac cagctcaact gatgaagaca
     1861 acagtgaaat gttcatgcaa acactttcaa ctgcattgtt taaacaaatt aaccgattgc
     1921 gcaataccca taggctcaca gtttgagtag ataccacggt gatttttact gcaaaggctc
     1981 tgaaaatggt tgataaatta actttatttt tactcgcaag catcttgtcc tccctcagtc
     2041 tcgctgatac caaacctcta cacaacagtt ggcgcaatga cagtggctat acagtgccat
     2101 ccaatggtgt cacaacaccc acatgctcat tggcaccttc gcaaaatagc actcatacca
     2161 acacctatca gggtagcgaa ctgatagagc gcttacaaca cagcttgaaa gagagcgaag
     2221 ccatcatcac tgatgattta gccagcaccc ccactagtga acaaaccatg tatcggaaca
     2281 acattcaggc aattaaagaa gttttgccct tgattcagcc caatactcgc tataccagtc
     2341 aggcttccct atgtggcaca cgacagcgaa gctggttagt catcaacaaa catttggcac
     2401 tcgaagaaca ttctgctacc gacgtggtaa cttatgtcat ttttcgccgc taagcccccc
     2461 caataagcag cattcttaac acctgattta tgggataaat tgggtttaaa gtaccgctac
     2521 atgagcaatc aaaaggaata tcacatgact ttgaaacaaa ccgtattgat ggcaatttgt
     2581 ttgtctgcca cggcatcctc tttcgccgcc atcaacaata atgccgccac tcacggacaa
     2641 tggcgcaaca gcgatggcaa taccatcagc gtcggtgccg atggggtgaa gcaatacgcc
     2701 gacaatgcag acgaatgccg cagcatgggc tacaaaatga cgggtgcccg cttcaaaggc
     2761 agcgaaatca aagccagcat gcaatctact ttggcatacg accgtgaaaa cctgcccgac
     2821 ctcaaagccg acaaccgtta tgcagagagt gccgatagcc tggccgtcaa cattcaaacc
     2881 atccagcgtc tgttgcccat ggtagacgcg aaaaaaacct acgcaggcat tgccatgcaa
     2941 tgcggtgacg gcagcagcga attgatattc ctcgacaatc acaatgccat cgaacaaaac
     3001 tttggcggcg gcgaaaccta ttacgagcat taccgcaaat aagccttcat ccatatccga
     3061 atcaaacaag cggcctgtaa acctttatag agtgtttaca ggccgcttgt ttgtatcttc
     3121 acatactttc aacagccgat tactgccatt gatttgcaca ttgccaccga caagagtttt
     3181 actctaagat gcattgggca tttgttccta accctatcaa cccgacaggc cacttatggt
     3241 tactgctgaa gacattttca aattggtttt tttcatttac tggggcgcca tgctatcggt
     3301 aataggactt gtatggttat ttgcctggaa attggtctct aagggaaaca agtggtgggt
     3361 aggcctgttg ggaactgctg cggctgcggc tatatttgtt ttgcccatgt tcaaacaaga
     3421 tgtcaaaacc aaacaagagg acaaagaact gcaagtcaga caagaaaaat atttggcagc
     3481 aaaagctgtg tttgatgagc gctgcaaaga tgcgggctac aaaatttaca aaacggttga
     3541 aaatgtggaa ggggtgacgt tattgaatgt atggcaaggt gaccctcgta atgaagacca
     3601 aatgtgggag tacgcaggat tacctagagc ttcgggtgag gatggttaca ttcaaggatt
     3661 tttatattgg agagcgtggg acttccaaac aaacgattat aattattatg gtaatggttt
     3721 tgtattagat tctaaatatc aacataaaaa tcttccaaac gaagtaaaac aacggtataa
     3781 aaatttaaat agttataagt atgtcgatgt agcgcagaat tctatttatc aacgttatca
     3841 atataaagaa ctttacgatc caagtgaaat ttccataaca acagtacaaa aaccaagcag
     3901 gtatacagtt gagtttgaaa atcccattat tcctgaagac cgcaatattt ggctagccac
     3961 gaccaaagcc ttcgttagag acacaaaaac aggaacctta ttgggagaag ccacttggta
     4021 cagtctacac ggtggacaag gggttaagaa atattcgacc acaggcattt gggacagagc
     4081 taaaacatgc ccagaaattg ccagcgccca agatgggcct attcagtatt ttgttttaaa
     4141 agtgttaaag cccaaacaat tccctcccca attgcaatcc aaaccagaag aaacaaatga
     4201 cgtggaaacc ttaaagccta aggctcaata atcacgggtg aagccagttc atgcgttttc
     4261 attgttgtaa tggttacaga ggtattgtcg gtaatgcgac tccatactat tgaagcggcc
     4321 tgtaaacctt tatagagtgt ttacaggccg cttgtttagg cttttcgcgc caaaatttgg
     4381 tggtgggtca tggtgaattc ggcattgggc aaggtcgccc atgtttgcca gccgtggctc
     4441 agttgttgca agctcgcgtc ggtcagcaag ccttgttctt gtgccagttt actaaaatcc
     4501 gatttgagca cgcgcacgct ccacatttcg ccccaccact gtgcgctgcc attggcggca
     4561 tggtaatgcc atggcgatgt ggaggttacg atgtcggtaa agcctactgc ttcacacaag
     4621 cgctgcaatt cagggccgat gttgggattg ccaccgctgt gggtggcaat ggcttggtac
     4681 acgtctagcc attgttgcaa tgcgggcgtg gcgggctgcc aagtcatgct gttgtaagcg
     4741 gtgtcgcgca cggccaagat gccattcggc ttcaataccc gcgccatttc gcgcaaggcg
     4801 cgcgcaggct cggccaagtg ttgcaacact tggtgggcat gcaccacatc aaagcttgcg
     4861 tcggcaaacg gcaatgcgta tgcgctcgcg gtttcaaact gcaaattatc gcgcgcatac
     4921 attgcttgcg cagccgcgat ggattcgggc gacacatcca ccgcgttgac ctgcccttgc
     4981 gccaacacat cggcgaaatc ggcgctgatg ctgccagggc cgcagcccac atcgagcaag
     5041 cgcatgtcgg cctgtaaatg gggcaacaag taagcggcgg aattggcggc gctgcggctg
     5101 cggtaagaat ttaaaacact gtcgtcatag ccgtggctgt agcgttcttg catggcttct
     5161 ccttggtatt gggtttaggg ctgaggcaag gcagcactgg caggcaaagg ggaaaactca
     5221 ctcgctaccc attcatagcc ttgtccttgt gggcgcacgt gccccaagcc agggaaaggc
     5281 atgtgtgcac ctgccaccca ccaaccgcgc gcagcggctt gttgcaacac cttgcgccgc
     5341 gatgccaccg attgtgcata gtcttggtct atttcgtacg tggcttcggg atgttgcaat
     5401 tgcacgctgt ggtaatgcac cacatcgccc cacagcatca gattgtcgcg ctcattctta
     5461 ctgggcaaca caaaagcgct gtggccaatg gtgtgaccag gcgtgggcaa cacttgcaaa
     5521 cctgcgggca aagtggttcc tgcactgaag gtatgcacgc gctgactttg taaataaggc
     5581 tgtaaagatt tgttcgccaa atcaaataaa aacgcaaatt tcttcggcgc tttgcgcttg
     5641 ttttgtgggc tttgccaata ctgcaactcc gccttgtcca accacacttg cgcgttggca
     5701 taaatgggtt tgccatcggt gctgagcaag ccgcacatgt ggtcgggatg gccatgggtc
     5761 aacaccaccg catcaatgtc ttcggtgcgg tagcctgcat agcgcaggtt ttcagccaca
     5821 tgacccaaat cacctgggaa acagttggct gtgcctgtgt ctaccaacac ttgctcgcgc
     5881 cccgtgtcga ttaaaaacgc gttaacggcg gtttgtaagc cgtctttggt ttcaggcaca
     5941 tagcggcctg taaggctttc ttgtacttgc tcaggagaaa tttgtgccag ttcactgcgc
     6001 ttcagcgcca ccacgccatc aaacaaggcg atgacgcgcg tctgaccgat ttgggctttg
     6061 tacacacctg gaatttgctg ctgtggcgcc aattgtgtag gggtttgcgc ccatgaagtg
     6121 gcactcaagc ccagcaaggc tgacaagctg taacgcaaaa cagtatgtga agtcatgtgg
     6181 atgttccgac caattgagat gccccattgt agccacagcc atggtttcgt ttcgctcaaa
     6241 tcaggcgaca aaacgctcac tccgaacgga tgtcgacagg aagcaagcca tagcgctgta
     6301 aaaaccgctg attgaacacg ctcaccgatt gatagccgca tttggcagca atggttttca
     6361 gcggccatgt gctggtgtac aacatgtcta aggcacaatt gaggcgggca tgcaaaatca
     6421 cctgcttcaa gctgctgcct tgtgcggcca agcggcggcg caaagtcgct ccactgaggt
     6481 gcaaatgctg ttcgatttcc gccgattgcc atgcatgcgc agggcggttt ctgaccaaat
     6541 cataaatttg agcggctagg tctttgctcg gcggcaataa cagtgctgag tggccttgtg
     6601 ccaccaagtg caacaccact tgggtcaagg ccaaacgcgc cgccacttca tcttccgcca
     6661 acaccgcacc cgcccatgcc gacaaggctt gcgcgcacac atcgattttt tcacaggcaa
     6721 tcgcaggctc tgtcgccatc gtcaacactg cctcgccttg caacaatctg gcggcctgta
     6781 acacggtttc ggaaatcgga atgcacacag tggcatagca cagcccatag tcgtcggtca
     6841 gattggccac gttgacagag cacgcacgct tgctgagcaa gatttcaccc acatgaatgt
     6901 tcaattgcgt gtcgccacaa cttatttgtt tgcgaccttg caacaccaca cccaattgcg
     6961 gctgcaaaat gtccaccgcc cgcaccacat gactgtggcg cgatgccacc accgcgcacg
     7021 tattggcgac ggtttgcaat tgtgccagca aggcataaac cgtttgcgaa ggtgcatcct
     7081 cagtcaccac atccatttat tctcttctcc cagtcctgaa tccataacat gcggcctgta
     7141 aacctaaaat tcaggcaaac acagtgtttc tatcccactg ctcagacaaa aatgccgcct
     7201 cagccacgct gaaacggcac aacttcagct gaatttcact catgccgatt cggttgccgc
     7261 ctctttggct actttttgtg tttcagcgat ggcggcatcg cgggcagcgg catcggcaaa
     7321 catcggactg gtgc
//
LOCUS       DAOMVK010000055         7315 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_2687_length_7315_cov_3.354821, whole genome shotgun sequence.
ACCESSION   DAOMVK010000055 DAOMVK010000000
VERSION     DAOMVK010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 7315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7315
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2687_length_7315_cov_3.354821"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="PK856_03125"
     CDS             complement(<1..400)
                     /locus_tag="PK856_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HRL01397.1"
                     /translation="MISKDLEQTLQEAYAYARVKRHELIGVEHLLLSLLDNETASQVL
                     VGCHANIELLQERLTESIVNDTPTVPEHLMNQVETQPTMGFQRVLHAAISHSQSAGKK
                     EVTGADVLVAIFSERESHAVYFLNQQSITRL"
     gene            complement(397..657)
                     /locus_tag="PK856_03130"
     CDS             complement(397..657)
                     /locus_tag="PK856_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003793401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HRL01398.1"
                     /translation="MPPKKYGVYLLNDDFTTMDFVMHVLQNVFFMEEARAFALMLVVH
                     EQGKGLCGVFNWDIATTKQSQVMDLAVEGEYPLQCTVEEATE"
     gene            934..1137
                     /locus_tag="PK856_03135"
     CDS             934..1137
                     /locus_tag="PK856_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HRL01399.1"
                     /translation="MATGTVKWFNDAKGFGFITPDEGGEDLFAHFSAINMNGFKTLKE
                     GQRVSFDVTEGPKGKQASNIQAA"
     gene            complement(1254..1838)
                     /locus_tag="PK856_03140"
     CDS             complement(1254..1838)
                     /locus_tag="PK856_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01400.1"
                     /translation="MRILIGVFVLVVLAACQSTKPNRQLDFKLDNGVTQSFDFTKRGA
                     LPADNGVYKVEGLGLMLYQQPQTKHVYFAWDVVVNMHAQDASKITVSQVLNNGQLQTL
                     LVDARPGLENAGKWRHLANGGKVNIMAKRWHAVTAGRPVSSVAWLSDAGNDSMFVFKI
                     DIEDTEYKHHVLYQPMVVSAESKVQYAKVIAAAE"
     gene            complement(1843..2349)
                     /locus_tag="PK856_03145"
     CDS             complement(1843..2349)
                     /locus_tag="PK856_03145"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HRL01401.1"
                     /translation="MEDLLLSDSVQKVHKVLNEQQVAHEFRVLSDSTRTAQMAADVLG
                     CTPSEIASSLIFENMETGALVLIVASGGHRVDLEYVSAQLGQPLQMAKGKRIKQEVGF
                     AIGGVPPLAHDVTLSTWLDVRLLQYATVWAAAGSPFAVFGVTPQELQRATGAKWMAVE
                     ASDAELLV"
     gene            2441..2917
                     /locus_tag="PK856_03150"
     CDS             2441..2917
                     /locus_tag="PK856_03150"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009116252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRL01402.1"
                     /translation="MIETQVALYVHPEFGRLQLHFQNEALVQVDLPFKPTYEGHFREL
                     PPEWQHKLDDYFTGRLQDFGCPVSPMGTAHDIKVWQAMIAIPYGQTRTYGDIAKEIGS
                     AAQAVGNACGRNPLPIIIPCHRIVGSSGIGGFSGSRGDETVSTKQWLLQHERTHAH"
     gene            2895..3782
                     /gene="xerD"
                     /locus_tag="PK856_03155"
     CDS             2895..3782
                     /gene="xerD"
                     /locus_tag="PK856_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003787248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HRL01403.1"
                     /translation="MNALTPTDYIDLLLEQLWLQERLSQNTLDAYRRDLHKLHDRLLT
                     EHVHWFNCDGVQLSHALFNNEEKPTSQARALSACKRLYAYFNEQNLKHQEPMFYLQRP
                     KIGRALPPIITEEQIDALLAAPDVETPHGLRDKALLELMYATGMRVSEAVGLQLGYMD
                     LQQGVVTTIGKGDKQRLIPLGEEACEWMAEYLLHARPSLLKQKPCDFVFVSQKRVGMT
                     RQLAWMIVDKYAEQIGIQHISPHKLRHAFATHLVNHGADLRVVQMLLGHADINTTQIY
                     THVANERLKHLITQHHPRG"
     gene            3804..4826
                     /gene="dinB"
                     /locus_tag="PK856_03160"
     CDS             3804..4826
                     /gene="dinB"
                     /locus_tag="PK856_03160"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HRL01404.1"
                     /translation="MNQPIRKIIHIDMDAFFASVELRDRPDLRDAPVVVAWEGARSVI
                     CAASYPARKFGLRSAMPLSVAKRLCPHVVCLPPHFEKYQQASRHIHRIFARYTDLIEP
                     LSLDEAYLDVTHNKQQMAYACDVATAIRHDILVETGLTASAGIAPNKFLAKIASDWRK
                     PNGQTTIAPQQIPTFLQHLSLSKIPGVGEKTQHKMAQLGLTMLTDLLTLSRGELVLYF
                     GKYGHRLYDLARGNETTLSVDAGLAEISAYIPKLCQEVWDSAAKHHYAARTVTVKLKT
                     ADFHTLTRSLSFSSDLHQIADFQAACMQLLQRMPQDLALRFRLIGVGLSQLHRTNEEG
                     HQYSMW"
     gene            4873..5700
                     /locus_tag="PK856_03165"
     CDS             4873..5700
                     /locus_tag="PK856_03165"
                     /inference="COORDINATES: protein motif:HMM:NF013652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRL01405.1"
                     /translation="MPTLNNHPEKRTYVTLLSDASYLQGVLTLHYSLMQTYPAYPLLV
                     LLNENASSHTQQALAQAKIPFKILPKLNYSEHSNQTMQQRGWPSALNNTADKLSVFTL
                     TEYDKVVYLDADMLVLKNIDYLFDYPHGSAVIDVANIIDITSNGNFTSEQYEYVSQFN
                     SGLFVFRPNLQDYQTCMDLLQTEVGFDQEILRSLWADWIHTPQRQLPQTCNIFASHLT
                     AYIQKGICGFTDVEVLHYTYLPKPWQQTQYNIHATRDFVYYIYREYLQKTLQAYQLT"
     gene            complement(5795..6241)
                     /locus_tag="PK856_03170"
     CDS             complement(5795..6241)
                     /locus_tag="PK856_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HRL01406.1"
                     /translation="MKKTIPIVTQAASACLCGHKVRYDGGDCATADVLAAEALPICPE
                     VMGGLPTPRAPAEIVGGDGHDVWAGRARVIGADGVDVTEAFKHGAELALQRLQAAHIE
                     TVYLKAKSPSCGQGQIYDGSFSGNLHDGDGVATALFKSHGIQVISV"
     gene            6352..6717
                     /locus_tag="PK856_03175"
     CDS             6352..6717
                     /locus_tag="PK856_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspG"
                     /protein_id="HRL01407.1"
                     /translation="MSLNRIATKQEVIARMVQGTDDVIAADAKIVRTDNGYWVAWHEH
                     TAAVIHEDAAEDEASFWVEGVATLDELLNMLTNGDFDEIEDFDGDDAEWDDVVLDSDA
                     HHDHEHGHDCDDPHCDHHH"
     gene            6727..>7315
                     /gene="coaBC"
                     /locus_tag="PK856_03180"
     CDS             6727..>7315
                     /gene="coaBC"
                     /locus_tag="PK856_03180"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009118552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HRL01408.1"
                     /translation="MTPHKHILLAITGGIAAYKSCELVRLLKKAGHEVTVAMSHSATE
                     FIHPNTFQALSGNPVLLDTHGSSGGNGMAHINLTREADVMLIAPASANTIAKIAHGIA
                     DNLISNLVAARTCPLVIAPAMNVQMWFNPANLRNIQQLKDDGVVILEPASGEQACGEI
                     GVGRMLEASQLADLLPDVWASKSLFGMKVMITTGAT"
BASE COUNT         1950 a         1965 c         1551 g         1849 t
ORIGIN      
        1 ccaaacgcgt gattgactgc tgatttaaaa aataaacggc atgtgattca cgttctgaaa
       61 aaatggccac caaaacatcg gctccagtca cttctttttt acctgcactt tgcgagtgtg
      121 aaatggccgc atgtaaaacc cgctgaaacc ccatggtcgg ctgtgtttcc acttgattca
      181 ttaagtgctc tggaacggtg ggcgtatcgt ttactatgct ttcggttaaa cgttcttgta
      241 aaagttcaat gttcgcatga caaccgacta acacttgaga tgcggtttca ttgtctagca
      301 acgacaataa caaatgttcc acaccaatta actcatgtcg cttcactcga gcgtaggcat
      361 aggcttcttg tagagtttgt tctaaatctt tcgaaatcat tctgtagcct cctccacagt
      421 acactgcaac gggtattcac cctccacggc caaatccatt acttgtgatt gcttggtggt
      481 ggcaatgtcc caattaaaca ccccacacaa tcctttacct tgttcatgca ccaccagcat
      541 caaagcaaaa gcccgagctt cctccatgaa aaacacattt tgtaaaacat gcatcacaaa
      601 atccatggtg gtgaagtcgt cgtttaataa atatacccca tatttttttg gaggcatcaa
      661 ttcatgtgcc aattctgttt caaattgtac tggcgccaca gttgtgtgat ggatagacat
      721 cgtaattggt tccactaatt tatgagaatg attctcaaga tcattgtatg ttttgtgttt
      781 tattgtcccc aattttaact tttttcgcaa actctgcttg acgcattggg gtaaacaacg
      841 gtaaaacgtc atcagtggta gggcaattga acaaaaattc atgccttgac tgcatttttt
      901 attatatttt gttttataga gttaggaagt ttcatggcaa caggtactgt taaatggttt
      961 aacgacgcta aaggttttgg tttcattact cctgatgaag gtggtgaaga tttgttcgct
     1021 cacttctcag caatcaacat gaatggtttc aaaactttaa aagaaggtca acgcgtatct
     1081 tttgatgtta ctgaaggccc taaaggcaaa caagcatcaa acattcaagc tgcataagct
     1141 agcgtgacat gcagccacca agcgggtggc ttttttatta aagcctgttg ctaagtttta
     1201 cccataaacc atgttacagc gaaaataagg gcacagaaat gtgctcttat tttttattct
     1261 gccgctgcaa tcacttttgc atactgcacc ttactttcgg cgctgaccac cataggctgg
     1321 tacaacacat ggtgtttgta ctcagtatct tcaatgtcta ttttgaacac aaacattgag
     1381 tcgtttcctg catcactcaa ccatgccaca ctgcttacag gccgacctgc ggtcaccgca
     1441 tgccaacgct tggccatgat atttactttg cctccattgg ccaaatggcg ccattttcca
     1501 gcattttcaa gtcccggtcg cgcatcaacc aacaaagttt gcaactgacc attgttcaat
     1561 acttgcgaca ccgtgatttt acttgcgtct tgcgcatgca tattgaccac cacatcccat
     1621 gcaaaataca catgcttggt ctgtggttgc tgatacaaca ttaagccgag gccttcaacc
     1681 ttatacacac cattatcagc aggcaacgca ccccgcttgg taaaatcaaa agattgggtc
     1741 acaccattgt ctaatttaaa atccagctgc cgatttggct tcgtgctttg acaagccgcc
     1801 aaaaccacca atacaaacac accaatcaaa atacgcatgg ccttacacca acaattcagc
     1861 atcagaagct tcaaccgcca tccacttggc acctgtggca cgctgtaact cttgcggcgt
     1921 cacgccgaaa acggcgaatg gagaacccgc tgccgcccac acggtggcat actgcaataa
     1981 ccgcacatcc agccaagtgc ttaaagtcac atcatgagcg agtggaggaa caccaccaat
     2041 ggcaaaacca acttcttgct tgatgcgctt acctttggcc atttgcaatg gctgacccaa
     2101 ttgtgccgat acatattcca aatcgactcg atgtccgcct gaagccacaa tcaacaccag
     2161 tgctcccgtc tccatatttt caaaaatcaa tgaactggca atttcactag gcgtacagcc
     2221 caatacatca gcagccattt gcgcggtgcg tgtgctgtca ctgagcacac gaaactcatg
     2281 cgccacttgt tgttcattga gcactttgtg gactttttgc acactgtcac ttaacagtaa
     2341 atcttccata ttttttcgct ttaatttgtg taaaaattgt ggcttgcaga ttattgttac
     2401 cgttttcaag tataatacaa caaacacaag gagtcgcgcc atgattgaga cacaagttgc
     2461 attgtatgtt catcctgaat ttggccgttt gcaattgcac ttccaaaatg aggcattggt
     2521 acaggtggat ttgccattca aacccactta tgaaggccat tttagggagc ttccgcctga
     2581 gtggcaacac aagcttgacg actattttac aggccgctta caagattttg gctgcccagt
     2641 cagccccatg ggcaccgctc acgacatcaa agtgtggcag gccatgattg ccattcctta
     2701 cggtcaaacc cgtacttacg gcgacattgc caaggaaatt ggttccgcag ctcaagccgt
     2761 aggcaatgcc tgtggtcgca atcccttacc catcatcatc ccttgccacc gcatcgtggg
     2821 tagctctggc ataggcggat tctcaggcag ccgaggtgat gaaaccgttt ccaccaagca
     2881 atggctatta caacatgaac gcactcacgc ccactgatta catcgactta ctgctggaac
     2941 aactgtggtt acaagagcgt ttaagccaaa acaccttaga cgcttaccgc cgtgatttac
     3001 acaaactaca cgacagactg ctcactgaac acgttcattg gtttaattgc gatggtgtgc
     3061 aattgtcgca tgcattgttc aacaatgaag aaaagcctac ttcccaagca cgggctttat
     3121 cggcctgtaa acgcttgtac gcctatttca acgaacaaaa tttaaaacac caggagccga
     3181 tgttttactt gcaacggccc aaaatcggcc gtgcgcttcc tcccatcatc accgaagaac
     3241 aaattgacgc cctacttgcc gcccctgatg ttgaaacgcc ccatggctta cgcgacaaag
     3301 cactgctcga attgatgtac gccacgggca tgcgggtttc cgaagccgtg ggtttgcagt
     3361 tgggttatat ggatttgcag caaggcgtgg tcaccaccat aggcaaaggc gacaaacagc
     3421 gcttgattcc tctgggtgaa gaagcctgtg aatggatggc cgaatacctg ttgcatgcta
     3481 ggccatcgct gttgaagcaa aaaccttgtg atttcgtgtt tgtcagccaa aagcgcgtgg
     3541 gtatgacacg acaattggcc tggatgatcg ttgacaaata tgctgagcaa atcggcattc
     3601 aacacatcag cccacacaaa ttgcgccatg cgtttgccac ccatttggtc aatcacggcg
     3661 ctgacttgcg cgtggtgcaa atgctgctcg gccatgccga catcaacacc acccaaatct
     3721 acacccatgt tgccaatgag cgcttgaaac acctgattac ccaacatcac ccacgcggct
     3781 aaaaccatgt ttacaggccg cttatgaacc agcccatacg caaaatcatc cacatagaca
     3841 tggatgcgtt ctttgcctca gtggaattgc gtgataggcc tgatttgcgt gatgcgcctg
     3901 tggtggtggc ttgggagggt gcgcgttcgg tgatttgtgc cgcctcttac cctgctcgaa
     3961 aattcggctt gcgctcggcc atgcctttga gtgtggccaa gcgtttgtgt ccgcatgtgg
     4021 tgtgcttgcc accgcatttt gagaaatacc aacaggcttc acgccacatt caccgcatct
     4081 tcgctcgcta taccgatctc attgagcctt tgtcactaga cgaagcctac ttggatgtca
     4141 cccacaacaa gcaacaaatg gcttatgcct gcgatgtggc caccgccata cgccacgaca
     4201 tcctcgtcga aacaggctta actgcttcag ctggcatcgc tccaaacaag tttttggcca
     4261 aaatcgcctc agattggcgc aaacccaatg gccaaaccac catagcccca caacaaatac
     4321 cgactttttt acaacacttg tctttgtcca aaattccagg cgtgggtgaa aaaacccaac
     4381 acaaaatggc acagcttggg ctgaccatgt tgaccgattt attgacctta agccgagggg
     4441 aattggtgtt gtattttggc aaatatggcc accgcctgta cgatttggca cgaggcaatg
     4501 aaaccactct cagcgtagac gcaggcttgg ctgaaatcag tgcttacatt cccaagttgt
     4561 gccaagaggt gtgggacagt gcagccaaac accactacgc cgcgcgaacc gtcaccgtca
     4621 aattaaaaac cgctgacttt cataccctca cccgttcctt gagcttcagc agtgatttgc
     4681 atcaaatcgc cgactttcag gccgcttgca tgcagttgtt acagcgcatg ccacaggatt
     4741 tggctttgcg ttttcgactg ataggcgtgg gcttgagcca attacaccgt accaatgaag
     4801 aaggccacca atattccatg tggtaatcgg atacaatcct ctcatccgca tccacatccc
     4861 tagcccatgc ccatgccaac tctaaacaac cacccagaaa agcgcactta tgtgaccttg
     4921 ctctctgacg caagctacct ccaaggcgtg ctcactttgc attactcatt gatgcaaacc
     4981 tatcccgcct acccactgtt ggtcttgtta aatgaaaatg cctcaagcca cacacagcaa
     5041 gcactcgctc aggccaagat tccatttaag attttgccaa aactcaatta cagcgaacac
     5101 agtaatcaaa ccatgcagca aagaggttgg cccagcgcac tcaataacac cgccgacaaa
     5161 ctcagcgtat tcacgctaac tgagtacgat aaagttgtgt atttagatgc cgacatgttg
     5221 gtgttaaaaa acattgatta tctgtttgat tacccgcatg gcagtgcggt catcgatgtc
     5281 gccaacatca tagacattac gtctaatggt aatttcacaa gcgaacaata tgagtatgtc
     5341 agccagttca actcaggctt atttgtgttt cgaccgaatt tacaagacta ccaaacgtgc
     5401 atggatttat tgcaaaccga agtgggtttc gaccaagaaa ttcttcgcag cctatgggca
     5461 gactggatac atactcccca acgccaatta ccgcaaacct gcaatatttt tgcctcacac
     5521 ctaaccgctt atattcaaaa aggcatttgt ggctttaccg atgttgaagt gttgcactac
     5581 acctatttac ccaaaccttg gcaacaaacc caatacaaca tccatgctac ccgcgacttt
     5641 gtctactata tttaccgcga gtatctgcaa aaaaccttgc aagcctatca actcacttaa
     5701 tccctgaaac cattccagta atggtttcaa aacacaaaca agcggcctgt aagtttacag
     5761 gccgcttgtt tgtataatag gcaactaaca tcactcaaac gctgattacc tgtattccat
     5821 ggcttttgaa taaagccgtg gccacaccat cgccatcatg caagttgcca ctgaagctgc
     5881 catcataaat ctgtccttgt ccacaagaag ggcttttggc tttcagatac actgtctcaa
     5941 tgtgagcggc ctgtaaacgc tgcaaagcca attctgcccc atgtttaaaa gcctcggtca
     6001 cgtccacgcc gtctgcaccg attactcgcg cgcgccctgc ccacacatca tggccatcgc
     6061 cgcccacgat ttcagcgggc gctcgcggcg tgggtaagcc acccatcact tctgggcata
     6121 tgggcaaagc ctcagccgcc aacacatcag cagtggcaca gtcgccacca tcgtacctga
     6181 ccttgtggcc gcacaaacac gcactggctg cctgagtgac tatgggtatc gtttttttca
     6241 tgggcaaatt ttagaatgaa tcaaagttta ttccaagcat gacgtgattc tcttgcctga
     6301 ttaaagcgtg taacatcgct acatctcagt ctcacagaca gaaagccaac tatgtcttta
     6361 aaccgtatcg ccaccaaaca agaagtgatt gcgcgcatgg tgcaaggcac agacgatgtc
     6421 attgccgccg acgccaaaat tgtgcgcacc gacaatggtt attgggtggc ttggcatgaa
     6481 cacaccgccg ctgtcatcca tgaagatgcc gcagaagatg aagccagttt ctgggtggaa
     6541 ggcgtggcga ctttggatga gttactgaac atgctcacca atggcgactt tgatgaaatc
     6601 gaagactttg atggcgacga tgccgagtgg gacgatgtgg tgctcgattc cgatgcccac
     6661 catgaccacg aacacggcca tgattgtgac gacccgcatt gtgaccacca ccactgacag
     6721 acagccatga caccacacaa acacatatta cttgccatca caggcggcat cgctgcttac
     6781 aaatcttgcg aattggtgcg cctgttaaaa aaagcgggcc atgaagtgac ggtggccatg
     6841 agccacagtg ccaccgaatt catccatccc aacacgtttc aagccttgag cggcaaccct
     6901 gtattgctcg atacccacgg cagctcaggc ggcaatggca tggcccacat caacctcaca
     6961 cgcgaagccg atgtgatgct gattgcgccc gcttctgcca acaccatcgc caaaatcgcg
     7021 cacggcatcg ccgacaatct catcagcaat ttggtcgccg cccgcacctg ccctttggtc
     7081 atcgcgcctg cgatgaatgt gcaaatgtgg ttcaacccgg ccaatttgcg caacattcag
     7141 cagctcaaag acgatggcgt ggtaatcctt gagcccgcat caggtgaaca agcctgcgga
     7201 gaaatcggcg tgggccgcat gttggaagct tcgcaattgg ccgatttatt gcccgatgtg
     7261 tgggcgagca aatcgctgtt tggcatgaag gtgatgatta ccacgggtgc cacgt
//
LOCUS       DAOMVK010000056         7189 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_2760_length_7189_cov_3.099804, whole genome shotgun sequence.
ACCESSION   DAOMVK010000056 DAOMVK010000000
VERSION     DAOMVK010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 7189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7189
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2760_length_7189_cov_3.099804"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /gene="bamA"
                     /locus_tag="PK856_03185"
     CDS             complement(<1..942)
                     /gene="bamA"
                     /locus_tag="PK856_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HRL01409.1"
                     /translation="MKLKLIAASIVGLGMSTAAWAVDPFTIQDIRIEGLQRTEPATIF
                     SYLPVQVGDRFSDAQAQAIIKDLYATGFFDDVQVETMGNQVLLTVVERPVITDLRVGG
                     GKAIKNDLIRTNLQNFGLATSQTYDEAIMSQAVEGLKREYANRGKYDAKITPKVARLD
                     RNRVEIELTIDEGETTTIKNIEFDGNEKFSDRTLRHQISHTESGLFTWFRKSNRFSQD
                     KMPADLESLSEFYQNRGYFDFKVTNAEVLPNPDEKSELNMKISVNEGQRYRFGQVSIA
                     GDLLDVPAEEINDLNKIKAGKWYERSKVMELVRDVQST"
     gene            complement(949..2289)
                     /gene="rseP"
                     /locus_tag="PK856_03190"
     CDS             complement(949..2289)
                     /gene="rseP"
                     /locus_tag="PK856_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HRL01410.1"
                     /translation="MIYLAAFVVAILLLVSLHEFGHYIVARWCGVKVLRFSVGFGKPF
                     WKKQRGDTEWCLAPIPLGGYVKMVDTREGEVAEADLPYAFDKQPPLKRIAIVVAGPLV
                     NLLLAVLLYVASFNLYGVTEVKPWVGTVEKDSIAAKAGFQVGDRITDINGKPVHDWMD
                     AQTELVIALQAGNVTVAVQDSQGKAQSRVMDVEANPASVEAATQGQGVGIWPIKVKSE
                     ISQVLPKSAAERAGLKAGDKVLSVNGQKLLDWNAWVEVIKASPGKDLNLEVQRQGQVL
                     NVMLRPDSEDLGQQLVGKAGVAASIDAVWEAQVKTKREVGVAESVTLAAHKTWNLSAL
                     TLKFLGKLIMGEASIKGISGPVSIGEMAGKSAMMGLETYIQFLAVISISLGVMNLLPI
                     PVLDGGHLLYYIIEWVRGKPLSEHVQTMGLKIGLCIMGLFMLVAFFNDFTRIFG"
     gene            complement(2314..3513)
                     /gene="ispC"
                     /locus_tag="PK856_03195"
     CDS             complement(2314..3513)
                     /gene="ispC"
                     /locus_tag="PK856_03195"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRL01411.1"
                     /translation="MKQVISILGATGSIGDSTLDLVRRHREQYEVFALAGNRQWQKLL
                     ELCTEFMPRYVVVMDEDVAHTLKQALQAASCSTEVLLGQEGLCDVASATEVTMVMAAI
                     VGAAGLPSAMAAAKAGKTILLANKETLVVSGQLFMDTVREHGARVLPVDSEHNAVFQV
                     LPPELTQHGRPITQDLADFGIQGIILTASGGPFWQRDVATMNDITPKEAVKHPNWSMG
                     HKISVDSASMMNKGLELIEARWLFNAKPNELTAVIHPQSVIHSMVRYRDGSVLAQMGT
                     PDMRTPIAYCMGEPQRIDAGVTPLNFTTLSQLTFAEPDLERFPCLRLAMEALAEGGDA
                     PCVLNAANEVAVAAFLQQQIGFTDIARVVEQVLAEANTQISHNVDDLLAKDAWARVAA
                     QQQLLRV"
     assembly_gap    3599..3698
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3699..4040)
                     /locus_tag="PK856_03200"
     CDS             complement(<3699..4040)
                     /locus_tag="PK856_03200"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRL01412.1"
                     /translation="MLKQRFVTAMVLIPLMVAMLFWSGDTVWLWFSGLIAITALWEYT
                     RLVGIVGLNQKLYLGLTAFIGLFIVRTDELNGVWQWLILLFWFVGMPYLLKRKQAITT
                     PWQKYALGLLLM"
     gene            complement(4047..4787)
                     /gene="uppS"
                     /locus_tag="PK856_03205"
     CDS             complement(4047..4787)
                     /gene="uppS"
                     /locus_tag="PK856_03205"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HRL01413.1"
                     /translation="MSSADSTVTDLIPRHIAVIMDGNGRWAKKRLLPRVMGHKRGLDA
                     LEATVKNCQELGVECLTVFAFSTENWRRPADEVGFLMNLFLQSLKKQVKRLHGNNIRL
                     RVIGKRDRFSVDIQSSIAEAEALTADNTGLLLSIAVDYGGHQDIVQAANQAIASGKTV
                     LTEEDIQTYISLNDVATPDLFIRTGGETRISNFLLWQLAYTELYFTETLWPDFDLAAL
                     QAAIHSFGQRERRFGRTSEQLPEHEQRK"
     gene            complement(4804..5361)
                     /gene="frr"
                     /locus_tag="PK856_03210"
     CDS             complement(4804..5361)
                     /gene="frr"
                     /locus_tag="PK856_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRL01414.1"
                     /translation="MINEIQKSAESKMQKSLEALKNNLAKVRTGRAHTGLLDQVSVDY
                     YGSPVPISQVANITLMDARTISVKPFETKMAGAIEKAIRDSDLGLNPSSMGDLIRVPM
                     PMLTEERRKDLIKVVRAEAEDGRVALRNVRRDANNEFKNLLKDKEISEDDARRGEEAI
                     QKLTDKSVAEVDKILAAKEADLMAV"
     gene            complement(5417..6139)
                     /gene="pyrH"
                     /locus_tag="PK856_03215"
     CDS             complement(5417..6139)
                     /gene="pyrH"
                     /locus_tag="PK856_03215"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRL01415.1"
                     /translation="MAEANLPYKRILVKLSGEALMGSDAFGINRDTIMQIVSQIKEVV
                     DLGVQVAVVVGGGNIFRGMAQSAKGMERATADYMGMLATVMNALALKEAFESIGVKAR
                     VQSALSMQQIAETYTRGKAMQYLEEGKVVIFGAGTGNPFFTTDTAAALRGIEMNCDIM
                     LKATNVDGVYNDDPKKNADAKRYQTVTFDEAIQKNLKVMDATAFALCREQKLNIKVFG
                     IFKDGALKRVVQGEDEGTLVHC"
     gene            complement(6306..7172)
                     /gene="tsf"
                     /locus_tag="PK856_03220"
     CDS             complement(6306..7172)
                     /gene="tsf"
                     /locus_tag="PK856_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRL01416.1"
                     /translation="MATITAKMVGDLRAATGLGMMECKKALVEADGDFDKAEEILRIK
                     SGAKAGKLAGRTAAEGIVVSSINGQVGALVEINCETDFVAKDDSFKGFAQAVADAVVA
                     ANPADVAALADAASGDSTVEELRKAVIAKLGENITVRRFVRYETADQLVAYMHGAKIG
                     VMVEFKGDAEVARQVAMHIAAARPQCVSADEVDPELVAKERHIYEEQAAQEGKPAEIV
                     AKMVEGRVRKYLAEVTLLGQQFVMNPDLTIEKFLKEKNTSVSAFTAYHVGEGIEKAVV
                     DYAAEVAAAAKI"
BASE COUNT         1915 a         1918 c         1389 g         1867 t
ORIGIN      
        1 ggtcgattgt acgtcacgca ccaattccat cactttactg cgttcatacc atttgcctgc
       61 tttgattttg ttcaaatcat tgatttcttc ggctggcaca tccaataagt cacccgcgat
      121 gctgacctga ccaaagcggt aacgctggcc ttcattcacc gaaattttca tgttcaattc
      181 agatttttca tctggatttg gcaacacttc tgcattggtc actttaaagt caaaataacc
      241 gcggttttga taaaactcgc tcaggctttc caaatcggcc ggcattttgt cttgtgagaa
      301 gcggttggat ttacggaacc aagtgaacaa accgctttct gtatggctga tttggtgacg
      361 caaagtacgg tcagagaatt tctcattgcc atcaaattca atgtttttga tggtggtggt
      421 ttcaccttca tcgatggtca attcaatttc gacacggttg cggtctaatc gggccacttt
      481 aggggtgatt ttggcatcgt atttaccacg attggcgtat tcacgtttca agccttcaac
      541 cgcttgcgac atgatggctt cgtcataagt ttgcgatgtc gccaaaccaa aattttgcaa
      601 attggtgcgt atcaaatcgt tcttaatcgc tttaccacca ccgacacgca aatcagtgat
      661 tacaggccgc tcaaccacgg ttaacaatac ttgattgccc atggtttcta cttgtacatc
      721 atcaaagaaa cccgtggcat acaagtcttt aataatggct tgtgcttgtg catcactgaa
      781 acgatcaccc acttgcacgg gtaaatagct gaatatggta gcaggttcgg ttctttgtaa
      841 accttcgata cgtatgtctt gaatggtgaa tggatccaca gcccaagctg cggtagacat
      901 gcctaaacca acaatacttg cagcaattaa ttttaatttc ataaagtttt atccaaaaat
      961 tcgcgtgaaa tcgttaaaga aggccacgag cataaacaaa cccatgatgc ataatccgat
     1021 tttcaaaccc atcgtttgca catgttcgct taagggtttc ccacgcaccc actcaataat
     1081 gtagtacaac aaatgtccac catccaatac tgggataggc aataagttca tcacacccag
     1141 gctgatgctg attacagcca gaaactgaat ataggtctct aaccccatca tggccgactt
     1201 acctgccatc tcaccaatag aaacagggcc tgaaatgcct ttgattgatg cctcacccat
     1261 aatgagcttg cctaaaaact tcaaagtcaa agcagacaaa ttccaagttt tatgggcagc
     1321 taaggtcacg ctttcagcca cacccacttc acgcttggtt ttgacttgcg cttcccatac
     1381 agcatcaatc gatgcggcaa ctccagcctt accaaccaac tgctgaccca aatcttctga
     1441 atctggtcgc aacatcacgt tcaaaacttg tccttgacgt tgcacttcca aatttaaatc
     1501 tttaccagga ctggctttga ttacttctac ccaagcattc caatccaaca atttttgacc
     1561 attaaccgac aagaccttat cgcctgcttt cagaccagca cgttcggctg cagacttagg
     1621 taaaacctga ctgatttcag atttgacttt aatcggccaa atacccacgc cttgaccttg
     1681 agtagcagct tctacgctgg cagggttggc ttcaacatcc atgacacggg attgtgcttt
     1741 tccctgggag tcttgtaccg ccaccgtcac attaccggcc tgtagagcaa tcaccaattc
     1801 ggtttgtgca tccatccaat catgtacagg tttaccattg atgtcagtga tgcgatcacc
     1861 gacttgaaag cccgctttgg cggcaataga atctttctca accgttccta cccatggctt
     1921 gacttcggtc acaccatata ggttgaaact cgccacatac agtagcacag ccaataataa
     1981 attcaccaac ggtcctgcca ccacaatggc gatgcgtttt aacggcggtt gcttgtcaaa
     2041 cgcataaggc aaatcggctt ccgccacttc accttcgcgc gtgtccacca ttttgacata
     2101 gccacccaat ggaattggtg ccaaacacca ctcggtgtcg cctctttgct ttttccaaaa
     2161 gggcttacca aagcccacac tgaaacgcag gactttgacg ccacaccagc gcgcaacaat
     2221 gtaatgacca aactcatgca agctgaccaa aagcaaaatt gccaccacaa aagcagccaa
     2281 ataaatcagt cctgtcatca caccttttcc agattaaacc cgcaacaact gctgttgtgc
     2341 ggcaacacgg gcccaagcat ctttggccaa taaatcatca acattatggc tgatttgggt
     2401 atttgcttca gccagaacct gttctaccac gcgcgcaatg tcggtaaaac cgatttgctg
     2461 ttgtaaaaat gccgcgacag ccacttcatt ggcagcattc aacacacaag gtgcatcgcc
     2521 accttcagcc aaagcctcca tcgccaacct caaacaagga aaacgttcca aatcaggctc
     2581 agcaaacgtc aattggctca aagtggtgaa attcaatggt gtcacaccag catctatgcg
     2641 ctgtggttca cccatgcaat acgcaattgg agtacgcatg tctggcgtgc ccatttgtgc
     2701 caaaaccgaa ccatcacggt agcgcaccat gctgtgtatc acgctttgtg gatgaatcac
     2761 agctgtcaat tcgttgggct tggcattaaa caaccaacgc gcctcaatca attccaagcc
     2821 tttgttcatc atgctggcag aatcaaccga tatcttgtgt cccatagacc aattggggtg
     2881 tttcaccgct tctttcgggg taatgtcatt catcgtagca acatcacgct gccaaaatgg
     2941 tcctcccgaa gcggttaaaa taatgccttg tataccgaag tctgctaagt cttgggtaat
     3001 cggcctacca tgctgcgtca actctggcgg caatacctga aaaactgcat tgtgctcact
     3061 gtcaactggc agcacccgtg caccatgttc gcgcaccgta tccataaaca attggcctga
     3121 aaccaccaaa gtttctttat tcgctaacaa tatggttttg cccgctttgg ctgctgccat
     3181 ggcggaaggc aagcctgccg cgcccacaat ggcagccatc accattgtta cctctgtagc
     3241 gctggccacg tcgcacaaac cctcttgccc caataacacc tcagtggaac aagatgcggc
     3301 ctgtaaagcc tgtttgagcg tgtgcgcaac gtcttcatcc atcaccacca cataacgtgg
     3361 cataaactca gtacacaact caagcaattt ttgccattgg cgatttcccg ccaaggcgaa
     3421 tacttcatac tgttcacggt gacggcgcac caaatccaaa gtactgtcac cgatgctgcc
     3481 agtggctcct aaaatactga tgacctgttt cataagcccc actgactcaa cacccaatac
     3541 acactcaata ccgcaatcaa tgaatccaca cggtcaaaca caccgccatg tcctggcann
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca tcaacagcaa acccaaggca
     3721 tatttttgcc aaggtgtggt gatggcttgt ttgcgtttca ataaatatgg catgccaaca
     3781 aaccagaaca gcaaaatcag ccactgccat acaccgttca attcatcggt acgcacaatg
     3841 aacaagccaa taaaggccgt caaacccaaa tacagttttt gattcaaacc cacaatgcca
     3901 accaagcggg tgtattccca caaagccgtg atggcaatca agccgctgaa ccacaaccac
     3961 acggtgtcac cagaccaaaa caacatggcc accatcaaag gaatcaaaac catggctgtg
     4021 acgaagcgct gtttcaacat attgtgttat ttccgttgtt cgtgttcagg caattgctca
     4081 ctggtgcgac caaaacggcg ttcacgctgt ccaaatgaat gaatagcggc ctgtaaagca
     4141 gccaaatcaa aatcgggcca cagtgtttcg gtaaaataca actcagtata ggccaattgc
     4201 cacaataaaa aattgctgat gcgggtttca ccacccgtgc gaataaacaa gtcgggcgtg
     4261 gccacatcat tcaaactgat gtaagtttgt atgtcttctt cagttaaaac cgttttacca
     4321 ctggcaatgg cctgattagc ggcctgtacg atgtcttgat ggccaccata atccaccgca
     4381 atcgacaata acaaacctgt attgtcagcg gttaaagcct ctgcctcagc aatgctgctt
     4441 tgtatgtcaa cactgaaccg gtcacgtttg ccaatgaccc gcaaacggat gttgttgcca
     4501 tgcaggcgtt tgacttgttt tttcaatgac tgcaaaaaca aattcatcaa aaacccgacc
     4561 tcatcggccg ggcgacgcca attttcagtt gaaaaagcga agacggttaa gcactccact
     4621 cccaactctt ggcaattttt taccgttgct tccagtgcat ccaagccccg cttatgcccc
     4681 atgacacgag gcaataagcg ctttttggcc caacgcccat tgccgtccat gatgacggca
     4741 atatgtcttg gtatcaaatc tgttacggta ctgtctgcac tgctcataat caaaacctcg
     4801 ggtttacact gccatcaaat ctgcttcttt ggcagccaaa attttgtcta cttcagcaac
     4861 acttttatcg gtcaatttct gaatggcttc ttcaccgcga cgcgcatcgt cttcagaaat
     4921 ctctttgtct tttaacaggt ttttgaattc attgttggca tcacggcgca cattgcgcaa
     4981 agccacacga ccatcttcag cttcagcacg cacgacttta atcaagtctt tgcggcgctc
     5041 ttcggtcaac ataggcatag gcacacgaat caaatcgccc atagatgatg gattcaaacc
     5101 caaatcagaa tcgcgtatcg ctttttctat cgcacccgcc attttggttt caaatggttt
     5161 cacactgatg gtgcgtgcgt ccatcaaggt aatgttagcg acttgactga taggcactgg
     5221 agagccgtag taatcaacgc tgacttggtc taacaaacct gtgtgagcgc ggcctgtacg
     5281 gactttggcc aaattgtttt tcaaggcttc cagcgatttt tgcatcttgc tttcggcact
     5341 tttttgtatt tcattgatca tttcaaaccc ttaattcttt aaatttaaca tgcttacagg
     5401 agggcttaga cccatcctag caatgcacca aagtgccttc gtcttcacct tgcaccacgc
     5461 gtttcaacgc gccgtctttg aagataccaa atactttgat gttcaatttt tgttcacggc
     5521 acaacgcaaa cgcggttgca tccatgactt tgagattttt ttgtatggct tcatcaaatg
     5581 tgacggtttg ataacgtttg gcatccgcat ttttcttagg atcatcgttg tacacgccgt
     5641 caacattggt ggctttgagc ataatatcac aattcatttc gataccgcgc aaagcagccg
     5701 ctgtgtctgt ggtgaagaaa gggttacctg taccggcacc aaaaatcacc actttaccct
     5761 cttctaagta ttgcatggcc ttgccacggg tgtaggtttc agcaatttgc tgcatgctca
     5821 gtgcagactg cacccgcgct ttcacgccaa tagactcaaa agcctctttc aaagccaagg
     5881 cattcatcac cgttgccagc atacccatgt agtctgcagt agcgcgctcc atgcctttgg
     5941 ccgattgtgc cataccgcgg aaaatattac cgccgccaac caccaccgcc acttgcacgc
     6001 ccaaatccac tacctcttta atctggctaa cgatttgcat gatggtatca cgattaatgc
     6061 caaatgcatc cgagcccatt aaagcctctc cagataattt gaccaatatg cgtttgtaag
     6121 gaagattggc ttcagccatg agggacacct ttaatagaaa gtcgttttaa acgaaagaat
     6181 tcaagctgag gcaggatgtt aaagcaatcg cttgcccatg acaaaccacc tcgctgaaat
     6241 tacaaaaatt ttacctaaaa aaacaccctg actcgaggaa tcagggtgct cttagtcaag
     6301 caggcttaga ttttagcggc agcagccact tcagcagcgt aatccaccac tgctttttca
     6361 atgccttcac ccacgtggta agccgtgaat gctgaaacag aagtgttttt ctctttcaag
     6421 aatttttcga tggtcaaatc aggattcatc acgaattgtt gacccaacaa agtcacttct
     6481 gccaagtatt tgcgcacacg gccttcaacc attttggcaa cgatttcagc aggcttgcct
     6541 tcttgagcag cttgttcttc gtagatgtga cgctctttgg ccaccaattc tggatccact
     6601 tcatcagcag acacgcattg tggacgagca gcagcaatgt gcatcgccac ttgacgagcc
     6661 acttcagcat cgcctttgaa ctcaaccatc acgccgattt tcgcaccgtg catgtaagcc
     6721 accaactgat cggcagtttc gtaacgaacg aaacgacgca cagtgatgtt ttcgcccaat
     6781 ttagcaatca cagctttgcg caattcttca acagtgctgt caccagaagc cgcatcagcc
     6841 aaagcagcaa cgtcagctgg attagcagcc acaactgcgt cagcaacagc ttgtgcaaaa
     6901 cctttgaagc tgtcatcttt ggcaacaaag tcagtttcgc agttaatttc taccaacgcg
     6961 cctacttggc cattgataga acttaccacg atgccttcag cagcagtacg gccagccaat
     7021 ttaccggctt tcgcgccaga tttgatgcgc aagatttctt cagctttgtc gaaatcacca
     7081 tcggcttcta ccaaagcttt tttacattcc atcatgccca agccagtagc tgcacgcaaa
     7141 tcgccaacca tttttgcagt aattgtagcc attgtttact ccttaaata
//
LOCUS       DAOMVK010000057         7175 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_2771_length_7175_cov_3.037921, whole genome shotgun sequence.
ACCESSION   DAOMVK010000057 DAOMVK010000000
VERSION     DAOMVK010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 7175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7175
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2771_length_7175_cov_3.037921"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(364..2076)
                     /locus_tag="PK856_03225"
     CDS             complement(364..2076)
                     /locus_tag="PK856_03225"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRL01417.1"
                     /translation="MKASQYFISTLKEAPAEAELPSHKLMLRAGLIKRVASGLYTWMP
                     MGLRVVRKVEAIVRDEMNQAGSIEVLMPAIQPAELWQESGRWEFYGKELLRLKDRHDR
                     DFCVGPTHEEVITDTMRREINSYKQLPKNFYQIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADFDSLKATYNVMYQAYCNVFDRLGLDYRPVAADTGSIGGTGSHEFQVLAASGE
                     DVIAYSTGSDYAANIELAPTLRQQGERVAAVEALTKIHTPNTKTIQALVDLLNISVEK
                     TVKSIVVEGEEESSLVLLLLRGDHEFNDIKAEKLAGVKSPLVMATPAAIKDAFGANGG
                     SLGPIGFKGKVYADYAVELLSDTVVGANEDDYHYTGFNFGRDAAEPEFVDLRNVINGD
                     ASPDGQGELKLVRGIEVGHVFQLRTKYAEAMNATFLDQNGKAQVMEMGCYGIGITRIV
                     AAAIEQNNDERGIIWTDAMAPFQAVIVPMNYKKSEAVREAADALYEQLKAAGVDVLLD
                     DRDERAGVLLNDSELLGIPHRVVIGDRGLKTGEVEYQARRDAESQAVALDAVATKVQA
                     ALGK"
     gene            complement(2479..3207)
                     /gene="trmB"
                     /locus_tag="PK856_03230"
     CDS             complement(2479..3207)
                     /gene="trmB"
                     /locus_tag="PK856_03230"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HRL01418.1"
                     /translation="MSEQEPDKHYQTSDIDNEVSHKRGIRSYVLRQGHMSDAQKAAMA
                     EGLPKWGIEYQPETIDLNAVFGRDNGKVFEIGFGMGTATAHIAKVLPEKDFIAIDVHA
                     PGVGNLLKLIDENELTNLRVMRYDAVEVVTHMLADESLAGIHVFFPDPWHKKRHNKRR
                     LIQTPFVEQLLPKLKIGGYIHLATDWEEYAVQMLEVLSGIDALQNTAADYAPTPEYRP
                     ETKFEARGIRLGHGVWDLVFKRVK"
     gene            complement(3297..4478)
                     /locus_tag="PK856_03235"
     CDS             complement(3297..4478)
                     /locus_tag="PK856_03235"
                     /inference="COORDINATES: protein motif:HMM:NF013729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HRL01419.1"
                     /translation="MKVFLSYAKEDKEFILECYEELKRKGFSPWMDEHDLLPGQAWDS
                     CLKANMKSTDVVLIFMSSVSVEKIGYVQREMKYFVDKRKDFPDDYIYIIPIQIDDCTV
                     PNLISSEIQFININKDLSGREWNKVLRSLGVAAEQRNIQKINNETSKKISVVLEEISE
                     SVFSFSGYDFKAIYPQVQTSLLGFNEINELVKNLIFNKLVIARSRQFDEIIDFERDND
                     EPMYFDIFQNEISGEIGYLKSYFMSVLFTEYEFKGGAHGSHYFYSRHYIIKDSKVSQI
                     SPFQIFSSDLLYEAITFIEDYCYQDLKKQISFRMNMLDDDLDNDWIKSGCSELIEQDE
                     IFIVKSNSLDVYFKPYSVTAYAFGDFIVSIPFYELNKWLNKGENSCYTKLHDFDSEFE
                     G"
     gene            complement(4600..5616)
                     /gene="ilvC"
                     /locus_tag="PK856_03240"
     CDS             complement(4600..5616)
                     /gene="ilvC"
                     /locus_tag="PK856_03240"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRL01420.1"
                     /translation="MKVYYDKDADLSLIKGKTVAIIGYGSQGHAHAQNLKDSGVNVVV
                     GLRKDGASWKKAENAGHKVLEVAEATKAADVVMILLPDESQPAVYRDSIAPNLKDGAS
                     LAFAHGFNIHYNQIVPPSNVDVIMVAPKGPGHTVRSEYLKGGGVPSLIAVYQDNSGKA
                     RDIALSYAAANGGTKGGVIETNFKEETETDLFGEQAVLCGGAVELVKAGFETLVEGGY
                     APEMAYFECLHELKLIVDLMYEGGIANMNYSISNNAEYGEYVTGVEVVTADTKAAMKK
                     ALHRIQSGEYAKMFINEGQLNYPSMTARRRMNAEHQIEIVGKQLRDMMPWIAKNKLVD
                     QEKN"
     gene            complement(5684..6175)
                     /gene="ilvN"
                     /locus_tag="PK856_03245"
     CDS             complement(5684..6175)
                     /gene="ilvN"
                     /locus_tag="PK856_03245"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HRL01421.1"
                     /translation="MRHILSILMENESGAMSRVIGLFSARGYNIDSLSVAMTEDPTLS
                     RMTIATRGDEEIIEQITKQLNKLIEVVKVIDLNDSHFVERELMLVKVRAVGKDRDEFL
                     RLAEIYRGRVVDVSERTYTIEVTGTTEKLDSFLQALTKSLILETVRTGAAGIGRGERM
                     LRI"
     gene            complement(6175..>7175)
                     /locus_tag="PK856_03250"
     CDS             complement(6175..>7175)
                     /locus_tag="PK856_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019389165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRL01422.1"
                     /translation="YYEANLAMQEADVVVAVGARFDDRVISVPSKFLATPKKVIHIDV
                     DPSSIAKRVKVNVPIVGDVKLVLQEMIALWKEQELMLNPGALEKWWKTIEAWRSRDCM
                     YFEQGDDVILPQYVVQTLAKVTGNQAIVTSDVGQHQMYAAQHYPFTRSRQWLNSGGLG
                     TMGVGLPYAMGAKLANPEQDVCCITGEGSIQMNIQELSTCYQYRIPVKVINLNNGYLG
                     MVRQWQEFYYHDRYSESYMDSLPDFVKLAEAYGHVGMRIEKPSDVEGALREALALKDR
                     MVFMDFITDRKQNVFPMVGNGKGLNEMVLPAHMRETVADSDVNDVNDRDYDTRSVP"
BASE COUNT         1912 a         1821 c         1401 g         2041 t
ORIGIN      
        1 ataatcaatg tgaaaatcgg tacgcagtgc atccacgccc tcatcggtag agggagtttt
       61 ggtgggcacc acgtcgtcac caatttgctt gaaacgctgc gcactcaata attgctgcca
      121 attcatcgcc ataagtcacc tcacttgaga ttgggtattt tacaggacgc atgccatgct
      181 gacaaagcgg cctgtaaacg cgtgtgactg ttttacggca tcgctgtgac catgcgatga
      241 caacaagacc actgcctaca tacccgcatc aacgcttccc cgctttatca catccatgcc
      301 tgcccacaaa aaaagccttg tgcagttggc acaaggcttt tgcatcactt caagcattca
      361 gcattacttg cccaatgcgg cctgtacttt cgtagccacg gcatccaatg ccaccgcttg
      421 tgattcggca tcgcggcgcg cttggtattc cacttcgccg gttttcaaac cacggtcacc
      481 gatgaccacg cggtgcggaa tgcccaacaa ttcagaatcg ttcagcaaca cgccagcgcg
      541 ctcgtcacgg tcgtctaaca acacatccac gcccgcggct ttcaattgtt cgtacaaagc
      601 gtcggccgct tcacgcacag cctctgattt tttgtaattc attggcacaa tcacggcttg
      661 gaacggtgcc atcgcatcgg tccaaatgat gccacgctcg tcgttgtttt gctcaattgc
      721 cgcagccaca atgcgcgtga tgccgatgcc gtagcaaccc atttccatca cttgcgcttt
      781 gccgttttgg tctaagaagg tcgcgttcat cgcttcagcg tatttggtgc gcaattggaa
      841 cacatggccc acttcgatgc cacgcaccaa tttcaactcg ccttggccat cagggctggc
      901 atcgccattg atgacgttgc gcaagtctac aaactcaggc tcggccgcat cgcgaccgaa
      961 attaaagcca gtgtagtggt aatcgtcttc gtttgcgccc accacggtgt cagacagcaa
     1021 ttccacagcg taatcggcat aaaccttgcc tttgaagccg atagggccca aagagccgcc
     1081 attcgcgcca aacgcatctt tgatggcagc aggtgtggcc atcaccaatg gtgatttcac
     1141 acccgccaat ttttcggctt tgatgtcgtt gaattcatgg tcaccgcgca acaacagcaa
     1201 gaccaatgaa ctctcttctt caccttccac cacgatggat ttcacggttt tttcaacaga
     1261 gatatttaac aagtccacca aagcttggat agtcttggtg tttggcgtgt ggattttggt
     1321 caaagcctca accgctgcca cacgttcacc ttgttggcgc aaagtcggtg ccaattcaat
     1381 gttggcagcg taatcagaac cagtgctgta ggcaatcacg tcttcaccgc tggcggccaa
     1441 cacttgaaac tcatgcgagc ctgtgccgcc tatgctgcca gtgtctgccg ccacagggcg
     1501 gtaatccaaa cccaagcggt caaacacgtt gcaatacgct tgatacatca cattgtaagt
     1561 ggctttcaat gaatcgaaat ctgcatggaa agaatacgca tctttcatca caaactcacg
     1621 cgcacgcatc acaccaaagc gcgggcgcac ttcatcgcgg aatttggttt gaatttggta
     1681 aaaattcttc ggcaattgct tgtaagaatt gatttcgcgg cgcatggtgt cagtaatcac
     1741 ttcttcatgg gttgggccca cgcagaaatc acggtcgtgg cggtctttca agcgcaacaa
     1801 ttccttgcca taaaattccc aacggccaga ctcttgccac aattcggcag gctgtatcgc
     1861 tggcatcaat acctcaatcg agcccgcttg gttcatttca tcgcgcacaa tcgcttcgac
     1921 tttgcgcacc acgcgcaaac ccataggcat ccacgtgtac aagccagaag ccacgcgctt
     1981 aatcaaaccc gcgcgcaaca ttaatttgtg acttggcaac tctgcttcag caggcgcttc
     2041 ttttaaagtt gaaataaaat attgactggc tttcatcaca ttctttccgc actctaatca
     2101 gtgctgttct aattgcttgt caagcagatt ttttatcttt attccttgac aaaccccaat
     2161 tcatacacag tgtcaaacaa gtgtcagaat ttagtcattt gtcagatatt actggcttga
     2221 caagacacat aatcatacta tccatttgat tttatttaat aatttttaaa agtctatatt
     2281 ttaggcaaag atgctgccaa gcttgatttt actgtctttt tggccatcac ccacaactta
     2341 tcaacatact tatccacaag gaatgtggac aattgccaaa agcttgatgg gacggcactt
     2401 agagctaaaa tacagcccag gtcacttatt ccaatcaacc aagattgccc gttgctcaaa
     2461 aaaaacatta atatcaaatt attttacacg cttaaatacc aagtcccaaa caccatgccc
     2521 taaacggatg ccacgggctt caaatttggt ttcaggacgg tactcaggcg tcggtgcata
     2581 atcggcagcc gtattttgca aggcatcaat gcccgacaac acttccaaca tctgcactgc
     2641 gtattcttcc caatcggtgg ccaaatggat atagccaccg attttcaatt tgggcagcaa
     2701 ttgttccaca aacggggttt gaatcagacg gcgcttgttg tggcgttttt tgtgccaagg
     2761 atcggggaaa aacacatgga tgccagccaa gctttcatct gccagcatgt gcgtcaccac
     2821 ttctaccgca tcgtaacgca tcacgcgcaa attggtgagt tcgttctcat caatcagttt
     2881 caacaaattg cccacgccag gcgcatgcac atcaatggca ataaagtctt tctcaggcaa
     2941 gactttggca atgtgtgctg tcgccgtacc catgccaaag ccaatttcaa acactttgcc
     3001 attgtcacgg ccaaataccg cgttcaaatc gatggtttcg ggctggtatt caatgcccca
     3061 cttgggcaga ccttcggcca tggccgcttt ttgcgcatca ctcatgtgac cttgacgcaa
     3121 aacatagctg cgtatgccgc gcttgtgact gacttcgttg tcgatatcgg aggtttggta
     3181 gtgcttatct ggttcttgtt cgctcatgct caaaactgca tttgatttca aagactgcca
     3241 ttatacaaaa taagcggcct gtaaggttac aggccgctta tttaattcct atatttttag
     3301 ccttcaaact ccgaatcgaa atcatgcaat tttgtataac aactattttc tcctttatta
     3361 agccatttat ttagctcgta aaatggtata gaaactataa aatcaccaaa tgcatacgca
     3421 gttacagagt aaggtttaaa atacacatct aaagaattgg acttaactat gaatatttca
     3481 tcttgctcta ttagttcaga gcaaccagat ttaatccaat cattgtccaa atcatcatcc
     3541 aacatattca ttctaaaaga aatttgcttc ttcaaatctt gataacagta atcttcaata
     3601 aacgtaatag cctcgtacaa caaatctgaa gaaaaaattt gaaaaggact gatttggctt
     3661 actttggaat ctttaattat atagtgtctc gaataaaaat aatggctccc atgcgcccct
     3721 cctttaaatt catactctgt aaataagaca ctcatgaaat aactctttaa atatccaatt
     3781 tcacctgaaa tttcattttg aaaaatatca aaatacattg gttcatcatt atccctctca
     3841 aaatcaataa tttcatcaaa ttgcctactt cttgcaataa ctaatttgtt aaaaattaaa
     3901 tttttaacta attcatttat ttcattaaaa ccaagtagtg aagtctgaac ttggggataa
     3961 atcgctttga aatcgtaacc cgaaaaagag aaaacacttt cggaaatctc ttccaaaact
     4021 accgagattt ttttacttgt ctcattatta attttttgaa tattcctctg ctcagctgca
     4081 acacctaaag aacgcaatac tttattccat tcacgtccag acaaatcttt attaatattg
     4141 ataaattgga tttcagatga aattagattt ggtacagtac aatcatcaat ttgaataggt
     4201 attatgtata tatagtcatc tggaaaatct tttcttttat ccacaaagta tttcatttct
     4261 ctttgaacat atccaatttt ctctacactg acagatgaca taaagattaa aaccacatca
     4321 gtgcttttca tatttgcttt tagacaacta tcccaagctt gccctggaag taaatcatgc
     4381 tcatccatcc aaggactaaa tcctttgcgc tttagttctt cataacattc aagaatgaat
     4441 tctttatctt cttttgcata actcagaaat actttcatat ccaaattttc caatacacaa
     4501 taaatactaa aaacaacatg atctttacca aacaagtatt aaagcggcct gtaaacattt
     4561 acaggccgct ttaagcttca aatcttcaaa gacttgagat tagtttttct cttggtctac
     4621 caatttgttt ttggcaatcc aaggcatcat gtcgcgcaat tgtttgccga cgatttcgat
     4681 ttggtgctcg gcgttcatgc ggcgacgggc agtcattgat gggtaattca actgaccttc
     4741 gttgatgaac atcttggcgt attcgccaga ttggatgcgg tgcaaggctt ttttcattgc
     4801 ggctttggta tcagctgtta ccacttccac accagtcacg tactcgccat actctgcatt
     4861 gttagagata gagtagttca tgttggcgat accgccttcg tacatcaagt ccacaatcaa
     4921 tttcaattcg tgcaagcact cgaagtaagc catttctgga gcgtagccgc cttcaaccaa
     4981 agtctcgaag cctgctttaa ccaattcaac cgcgccaccg cacaatacgg cttgttcgcc
     5041 gaacaagtcg gtttcggttt cttctttgaa gttggtttca atcacgccac ctttggtgcc
     5101 gccattggct gctgcgtaag acaaagcgat gtcacgggct ttaccagaat tgtcttgata
     5161 aaccgcgatc aacgatggca cgccgccgcc ttttaagtat tcgctgcgta ctgtgtggcc
     5221 tgggccttta ggcgcaacca tgatcacgtc tacgtttgat ggtggcacga tttggttgta
     5281 atggatgtta aagccgtgag caaaggccaa agaagcgcca tctttcaagt taggagcgat
     5341 agagtcgcgg taaacagctg gttggctttc gtctggcaac aagatcatta ccacgtcagc
     5401 ggctttggtg gcttctgcca cttccaatac tttgtgacct gcgttctcgg cttttttcca
     5461 agaagcgcca tctttgcgca aacccactac cacgttcaca cctgaatctt tcaggttttg
     5521 agcgtgtgcg tgaccttgtg aaccataacc aataatggca acggttttac ccttgatcaa
     5581 agataaatcc gcgtctttat cgtaataaac tttcattgtg tttcctttta tttcaaacag
     5641 gtgttatata aggcggcctg taaacgactg ccttgtgtgt caattaaatt cgcaacattc
     5701 tttcaccgcg cccgatgcca gcagcacctg tgcgcacggt ttccaaaatc aggcttttcg
     5761 tcaaggcttg caagaacgaa tcgagcttct ctgtggtgcc cgttacttcg atggtgtaag
     5821 tgcgctcact cacatccacc actcggccac ggtagatttc tgccaaacgc aagaattcat
     5881 cacggtcttt gcccacagcg cgcactttta ccaacatcaa ttcacgctca acaaaatgtg
     5941 aatcattcaa atcaatcact ttaaccactt caatcaattt gttcaattgc ttggtgattt
     6001 gttcaatgat ttcttcatcg ccacgtgttg caatggtcat gcgcgacaaa gttgggtctt
     6061 cggtcatcgc cactgacaat gagtcaatat tgtaaccacg ggcagaaaac agaccaatca
     6121 cacgggacat cgcgccagat tcattttcca ttaaaatcga taaaatgtgt ctcattatgg
     6181 cacgctccga gtatcgtaat cacggtcgtt aacgtcgtta acatctgagt ctgctacggt
     6241 ttcacgcata tgcgctggca gaaccatttc gttcaagcct ttgccattgc ccaccatagg
     6301 gaacacgttt tgtttgcgat cggtaatgaa gtccatgaac accatgcggt ctttcaatgc
     6361 caatgcttcg cgcaaagccc cttcaacatc gcttggtttc tcaatgcgca tgcccacgtg
     6421 accataggct tctgccaatt tcacgaaatc tggcaatgaa tccatgtacg attctgagta
     6481 gcggtcgtgg tagtagaatt cttgccactg acgtaccatg cccaaataac cgttattcag
     6541 gttaatcact ttcacaggaa tgcggtattg gtagcaagta gacaattcct gaatgttcat
     6601 ttgtatcgag ccttcacccg taatacagca tacatcttgc tcaggattcg ccaatttggc
     6661 acccatggca taaggcaagc ccactcccat cgtgcccaaa ccaccagaat tcaaccattg
     6721 acgtgaacgg gtaaacggat agtgttgtgc agcatacatt tggtgttggc ccacgtcaga
     6781 agtcacgatg gcttgattgc ccgtcacttt ggccaaggtt tgcaccacgt attgcggcaa
     6841 aatcacatca tcgccttgct cgaaatacat gcagtcacgg ctgcgccaag cttcgatggt
     6901 tttccaccat ttttccaatg cgcctggatt gagcatcaat tcttgctctt tccacaaggc
     6961 aatcatctct tgcaacacca gtttcacatc gcccacgatt ggcacgttta cttttacgcg
     7021 cttggcaatg ctcgatgggt ctacatcaat gtggatgact tttttcggcg tggccaagaa
     7081 tttgcttggt acggaaatca cgcggtcgtc aaaacgagcg cccaccgcca ccaccacgtc
     7141 ggcttcttgc atggccaaat tggcttcgta ataac
//
LOCUS       DAOMVK010000058         7103 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_2814_length_7103_cov_3.702043, whole genome shotgun sequence.
ACCESSION   DAOMVK010000058 DAOMVK010000000
VERSION     DAOMVK010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 7103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7103
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2814_length_7103_cov_3.702043"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /gene="metK"
                     /locus_tag="PK856_03255"
     CDS             complement(<1..1060)
                     /gene="metK"
                     /locus_tag="PK856_03255"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HRL01423.1"
                     /translation="MGEYLFTSESVSEGHPDKVADQISDAILDAILAQDPTARVAAET
                     LVNTGLAVLAGEVTTTAVVDYIKVARQTITDIGYNRSELGFDANGCAVLVGYDQQSPD
                     IAQGVNEGEGIDLDQGAGDQGLMFGYACDETDTLMPFAIYYSHRLMQRQSELRKNGTL
                     PWLRPDAKAQLTVVYDEETGQVKRIDTVVLSTQHDPDIDHKELEQAVIEHIIKPVLPE
                     NMITAETKYLVNPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVD
                     RSAAYACRYVAKNIVAAGLAKQCQIQVSYAIGVAEPTSISVHTFGTGAVSEAKLTELV
                     RAHFDLRPKGIVKMLDLLR"
     gene            1144..2037
                     /locus_tag="PK856_03260"
     CDS             1144..2037
                     /locus_tag="PK856_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRL01424.1"
                     /translation="MQVMLRLIFQFLAVLPLPLLHALGNFFGWLAYHFSAKTRSLTAD
                     NIRISQIAQDYQSMNDLVKSCLQETAKSGLELPIAWCRDSQDVVKLFQQVHGWEHVEA
                     AIANEQGLLLITPHIGSYDLAGRFISERLPFPLTAMYRPPKLAWLEPIMQAGRERDQG
                     HIAPANAQGVKIVLKALKNKEATIVLPDQVPTGGDGVWAHFFGQEAYSMTLAARLAQM
                     NDVATLFFVGERLPHGRGFALHVYPLEGEMGRDKLANVETINRNVEKLIRLFPSQYLF
                     SYNRFKTPAGAEPKPSSGLKD"
     gene            2039..2911
                     /locus_tag="PK856_03265"
     CDS             2039..2911
                     /locus_tag="PK856_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003741426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HRL01425.1"
                     /translation="MRIAFAFLYLIHLLPIRLIHALAKPVGWLCYYLVKPRRKVGQIN
                     LAHCYPELSEAERTHILKQHFYHMACLILEYGVCWYSKAERLHKLVRYVDKHHFDTVL
                     ERGDKVIILYPHFTAFEMALFKLNQDVPLTSMYGHQKNEVLDKQILAGRHRYNNVFLV
                     ARQEGLRGLIRMMRKRPEAPFTYLPDQDFGRKDSVFVNFFGIPTATITGLSRIAQMTD
                     AKIIPAIPTRLNDGTFELRFYPEWDNFPTADPLADTQRMNDFIEARVREQPAQYFWLH
                     KRFKTRPEGEKNWY"
     gene            2928..3461
                     /locus_tag="PK856_03270"
     CDS             2928..3461
                     /locus_tag="PK856_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01426.1"
                     /translation="MYTWNMPNTFPQSKACLYNQTNVDDYFSLQTGCELISVPACVYF
                     DSKTSRDFLSQHDYLPNNTGSLLLSGRLSDWLQQTVADCVQPIPAVIQCQDGPLQDYW
                     VVNIVQQVAAINEAKSMAGSFGIYHKLVYLSETDDSLFLARCKGNLAEVLVSNALKQA
                     LQQTDFRILKFYTCPTS"
     gene            3517..4044
                     /locus_tag="PK856_03275"
     CDS             3517..4044
                     /locus_tag="PK856_03275"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRL01427.1"
                     /translation="MLNTDRLILRHWQPSDLDNLHRLCSNIDVMRYFPNIQDRAASRQ
                     MLHRLQNAFLWHPDYGVWVLELKQTGEFVSMLGLQNCNLPFDFMPKIEILWRLLPKFW
                     SQGLALEAALCVQDYALNHLRLPALTAYTSYPNLPSQRLMERLGMHASLSFTHPMLPA
                     HHRLSRHIAYFSHVH"
     gene            complement(4120..4458)
                     /locus_tag="PK856_03280"
     CDS             complement(4120..4458)
                     /locus_tag="PK856_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01428.1"
                     /translation="MTQKGSRSIVVDGVSYRWMVRPKPTYMQEVFACPMTFAVVADGK
                     LNTTLWVVVGACRPEACKIVVMARTLSITPAIVTEAIRMALQQGWQAQHAGPSFELCL
                     ILDSELLMKA"
     gene            complement(4509..4808)
                     /locus_tag="PK856_03285"
     CDS             complement(4509..4808)
                     /locus_tag="PK856_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HRL01429.1"
                     /translation="MTTAMYVLLLVAVVLANSPFLSQKWFGVLPLPRKHFGHHLLELA
                     VGFALTGVLAYVFEAQAGPVHQKDWEFFVVTLALFVVFGFPGFVWRYFWHSRNHA"
     gene            complement(4898..6358)
                     /gene="nuoN"
                     /locus_tag="PK856_03290"
     CDS             complement(4898..6358)
                     /gene="nuoN"
                     /locus_tag="PK856_03290"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HRL01430.1"
                     /translation="MNWAELNLTPAIPEITLLCVLGLVLLIDLFLNDRNRWVTYVLSL
                     VGIVVVAATQVMVWHGQPEAAFGNLFVNDGISQLAKLAMYAGVFVLFVYSRRYLANRE
                     IFKGEFYTLTFFALLGMQVMVSANHFLTLYVGLELLSLALYAMVALRRDNLKATEASL
                     KYFVLGALASGLLLYGISFIYGATGHLQLNDVLNAIQSKADVNPWLLTIGMIFIVVGV
                     AFKLGVVPFHMWVPDVYEGAPTAVTAFVGTAPKIAAMVFAFRIVGTALPTLAADWTQM
                     FALLAVASLLLGNLAAIMQTNIKRMLAYSTVSHMGFVMLGFIGGFQNGGYAAALYYAI
                     TYMVMGLVAFGVLMALSNKDHECENISDLAGLNQKNPYLALMMLVSMFSMAGIPPLMG
                     FYAKLSILRSLIATGEGMYIWVSVFAVIMSLIGAFYYLRVVKVMYFDEPTNNIKGDFK
                     VDAWILLAVNSLALVLLGVFPSSVVNWCIEALKLTM"
     gene            complement(6370..>7103)
                     /locus_tag="PK856_03295"
     CDS             complement(6370..>7103)
                     /locus_tag="PK856_03295"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRL01431.1"
                     /translation="GAYGFLRFILPIVPDASHFFAPVVIVLSLVAVVYVGMVALVQTD
                     MKKLVAYSSISHMGFVTLGLFMFVDGTLNDWGLKGAIMQMISHGFVSAAMFMCIGVMY
                     DRMHTRNIADYGGVVNTMPKFAAFMMLFAMANAGLPATSGFIGEFMVIMGATEYNFWV
                     GMLAASTLIFGAAYTLWMYKRVIFGAIANEEVRQLKDVNGRELLILIILTVAVLGMGL
                     YPEPFIAVVHQAANTLIEQVAQSKI"
BASE COUNT         1979 a         1863 c         1540 g         1721 t
ORIGIN      
        1 ggcgcaacaa atccaacatt ttcacgatgc ctttagggcg caaatcaaag tgcgcacgta
       61 ccaattcggt caatttcgct tctgataccg cgcctgtacc aaatgtgtgc acactgatag
      121 aggtgggctc cgccacgcca atggcgtaag acacttgaat ttggcactgt ttcgccaagc
      181 ctgcggccac aatgtttttg gccacatagc ggcaagcata ggcagcagaa cggtctactt
      241 tggttgggtc tttgcctgag aatgcgccac caccatgtgg cgctgcgccg ccataagtat
      301 cgacaatgat tttacggcct gttaaaccac aatcaccttg tgggccacca atcacaaagc
      361 ggcctgtagg gttcaccaag tatttggttt ctgcggtaat catgttttct ggcaaaacag
      421 gcttgatgat gtgttctatc accgcttgct ctagctcttt gtggtcgata tcagggtcgt
      481 gttgggtaga caacaccacc gtgtcgatgc gtttcacttg gccggtttct tcatcgtaaa
      541 ccacggtcaa ttgggctttg gcatcaggac gcaaccatgg caaagtgcca tttttgcgca
      601 attcgctttg gcgttgcatc aaacggtgtg aatagtaaat ggcaaacggc atcaaggtat
      661 cggtttcgtc acaagcataa ccaaacatca agccttggtc gcccgcgcct tgatccaaat
      721 cgatgccttc gccttcgttc acgccctggg caatgtcagg cgattgttgg tcgtaaccca
      781 ccaataccgc acaaccattg gcatcaaagc ccaattcaga gcggttgtaa ccaatgtcag
      841 taatggtttg acgcgccact ttgatgtaat ccaccaccgc agtggtcgtt acttcgcccg
      901 ccaataccgc caaaccggtg ttcaccaagg tttccgccgc cactcgtgcg gttggatctt
      961 gtgccaaaat ggcatccaaa atcgcatctg aaatttggtc agccactttg tctggatggc
     1021 cttcagatac cgattctgaa gtaaataaat attcgcccat tgcgcttctt tcgtattcaa
     1081 aaactgtttg gattgcctgt gctagaataa gccattccga taaatggtga caacacacac
     1141 aatatgcaag ttatgcttcg gctgattttt caatttttgg cggttttgcc cttaccattg
     1201 ctacacgcac tgggcaattt tttcggttgg ctggcgtatc acttcagcgc caaaacacgt
     1261 tcacttaccg ccgacaacat tcgcatcagc caaatcgccc aagactatca aagtatgaat
     1321 gacttagtca aatcgtgcct acaagaaacg gccaaatctg gcttggaatt gcccattgct
     1381 tggtgccgcg attcgcaaga tgtggtgaaa ttgttccagc aagtccacgg ctgggaacat
     1441 gttgaagccg cgattgccaa cgaacaaggc ttgttgctta tcacgccaca tattggcagc
     1501 tatgatttgg caggacgttt catctctgaa cgcctgcctt ttccactcac tgccatgtac
     1561 agaccgccca aattggcgtg gctagaaccc atcatgcaag caggccgcga gcgtgaccaa
     1621 ggccatatcg ctcctgccaa tgcacaaggg gtgaaaatcg tgctcaaagc cttgaaaaac
     1681 aaagaagcca ccatcgtgtt accagaccaa gtgcccacag gtggcgatgg agtgtgggca
     1741 catttctttg gccaagaggc ttattccatg actttggccg cacgtttggc acaaatgaat
     1801 gatgtggcca ccttgttttt tgtcggtgag cgtttgccac acggccgtgg cttcgcctta
     1861 cacgtttacc cattggaagg tgaaatgggt cgcgacaaac tcgccaatgt ggaaaccatc
     1921 aaccgcaatg tggaaaaact catccgtttg tttcctagcc aatacctgtt cagctataac
     1981 cgttttaaaa cgcctgcagg tgccgaaccc aagccctcaa gcggcctgaa agattgatat
     2041 gcgcattgct tttgcatttt tatacctgat acacctctta cccatacgct tgatacatgc
     2101 actggccaag cctgtgggtt ggttgtgtta ttacttggta aaaccacggc gcaaagtcgg
     2161 ccagatcaat ctggcccatt gctatcctga actgtcagaa gccgaacgca cccacatcct
     2221 caaacagcat ttttatcaca tggcgtgttt gattttggaa tacggcgtgt gttggtacag
     2281 caaagcggaa cgcttgcata aattggtgcg ctatgtggat aaacaccact ttgatacggt
     2341 gttggaacgt ggtgacaaag tgatcatctt gtatccacac ttcaccgcat ttgaaatggc
     2401 tttgttcaaa ctcaatcaag atgtccctct caccagtatg tatggtcacc aaaaaaatga
     2461 agtgttggac aaacagattt tggcagggcg ccaccgttac aacaatgtgt ttttggtggc
     2521 gcgccaagaa ggtttacgtg gcctcattcg catgatgcgc aagcgccctg aagcaccgtt
     2581 tacctattta cctgaccaag attttggtcg caaagactcg gtattcgtga atttctttgg
     2641 catccctacc gccaccatca caggcttatc acgcattgcg caaatgacgg atgctaaaat
     2701 catccccgcc atccctaccc gcttgaacga tggcacgttt gagctgcgtt tttaccctga
     2761 gtgggataac tttcccactg cagacccatt ggccgatacc caacgcatga atgatttcat
     2821 cgaagctcgg gtgcgcgaac agcctgcaca atacttttgg ctgcacaaac gcttcaaaac
     2881 ccgccctgaa ggcgagaaaa actggtatta gcgagcgatt cattaccatg tatacctgga
     2941 acatgcccaa tacttttcct cagtccaagg cctgtctgta taaccagacc aatgtcgatg
     3001 actatttctc cttacaaaca ggatgtgaac tgatatcggt accggcttgt gtgtattttg
     3061 acagtaagac cagccgtgat tttttaagcc aacacgatta tttgcccaac aacaccggca
     3121 gtttgttgct ttcaggccgc ttgagcgact ggttacagca aaccgtagcc gattgcgtgc
     3181 aacccatccc tgcagtgatt caatgtcaag atgggccact tcaagactac tgggtggtga
     3241 acattgtgca acaagtagcg gccattaacg aagccaaaag catggctggc tcttttggga
     3301 tttatcacaa attggtgtat ttatcggaaa ccgatgacag cctgtttttg gcccgatgca
     3361 aaggaaatct ggcagaagtg ttggtatcga atgccctcaa acaggcatta caacaaaccg
     3421 attttcgtat tttaaaattt tatacttgcc caacttcatg atggtttaca tcctacagcc
     3481 cactttttgg ccgcacagcg gtaaatggaa ggtgtcatgt taaacaccga ccgtctgatt
     3541 ttgcgccatt ggcagcccag tgacttagac aatctgcacc gtttgtgcag caatattgac
     3601 gtgatgcgct attttcccaa tattcaagac cgcgctgcca gcagacaaat gctgcatcgc
     3661 ttgcaaaatg ctttcttatg gcaccctgat tatggggttt gggtgctgga gctgaaacaa
     3721 acaggtgaat ttgtcagcat gttgggttta caaaattgca atttgccatt tgattttatg
     3781 cccaaaatcg agatattgtg gcgcttgctg ccgaagtttt ggtcgcaagg cttggccttg
     3841 gaagcagcct tgtgcgtgca agactatgct ttaaatcatt tgcgcctgcc tgcactcacg
     3901 gcttacacca gctaccccaa cctgccctca caacgcttga tggaacgctt gggcatgcac
     3961 gctagtttga gcttcaccca tccaatgttg cctgcacacc accgcttgag ccgccacatc
     4021 gcttatttct cccatgtgca ctaattcaag catccaatac aagataagcg gcctgtaaca
     4081 tggtttacag gccgcttgtt tattgtaaac caatgtggtt catgctttca tcaacaattc
     4141 tgaatctaaa atcaaacata attcaaaact aggcccagca tgttgagctt gccaaccttg
     4201 ctgtaaggcc atgcgtatgg cttctgtcac aatagctggg gtaatggaca aagttcgggc
     4261 catgacgaca attttacacg cttcaggacg gcaagcaccc accaccaccc ataaagtggt
     4321 gttgagcttg ccgtcagcga ccacagcaaa cgtcatgggg catgcaaaca cttcttgcat
     4381 ataagtgggt ttcggccgca ccatccaacg gtaactgaca ccgtccacca caatggaacg
     4441 actgcccttc tgtgtcatgc ccatataccc tctcttagct ctgtcacttt cacctgctta
     4501 caagtcgctt aagcatggtt gcggctgtgc cagaaatagc gccacacaaa cccagggaaa
     4561 ccaaacacca caaacaaggc gagggtgacc acgaaaaact cccaatcttt ctgatgcaca
     4621 ggccctgctt gggcttcaaa cacataggcc agcacacctg ttaaggcaaa acccacggct
     4681 aactccagca aatggtgtcc aaaatgcttg cgcggcaagg gtaatacccc aaaccatttt
     4741 tggctgagaa atgggctgtt tgccaacacc accgccacca acagcagcac atacatcgct
     4801 gtcgtcataa caactccttc aactgaatca gactgcacta tagcaaaacg gcaaccacta
     4861 gggttgccgt aacacaaaca tcagtgaaat tgcatcatta catggtcaat ttcaaggctt
     4921 cgatacacca gttcaccacc gatgatggga acacacccag caataccaga gccaagctgt
     4981 tcactgccaa caaaatccaa gcatcgactt tgaagtcgcc tttaatgttg ttggtaggct
     5041 cgtcgaagta catcactttg accacgcgca agtagtagaa cgcgccaatc aaagacatga
     5101 tgactgcaaa cacagacacc cagatgtaca tgccttcgcc cgttgcaatc aatgaacgta
     5161 agatgctcaa tttggcgtag aagcccatca atggaggaat accagccata gagaacatgg
     5221 aaaccaacat catcaaagcc aagtagggat ttttttggtt caagccagcc aaatcgctga
     5281 tgttttcaca ttcgtggtct ttgttagaca aagccattaa cacaccgaac gctaccaaac
     5341 ccattaccat gtaagtgatg gcgtagtaca aagccgcagc gtaaccaccg ttttggaaac
     5401 caccgatgaa acccaacatc acaaaaccca tgtgggacac cgttgagtaa gccaacatac
     5461 gtttgatgtt ggtttgcatg atggcggcca agttaccaag cagtaatgaa gccactgcca
     5521 acaaggcaaa catttgtgtc cagtcggctg ccaaagtagg caaagcagtg ccaacaatgc
     5581 ggaaggcaaa caccatggcg gcaattttag gtgctgtacc gacaaatgca gtcaccgcag
     5641 taggtgcacc ttcgtatacg tcaggtaccc acatgtggaa aggcaccaca cccaatttga
     5701 atgccacgcc caccacgatg aaaatcatac cgatggtcaa caaccatgga ttcacatctg
     5761 ctttggattg gatggcattc aacacatcgt tcaattgcaa gtggcctgtg gcaccgtaaa
     5821 tgaatgaaat accgtataac aacaaacccg atgccaaagc acccaataca aagtatttca
     5881 acgatgcttc agtggctttc aagttgtcgc ggcgcaaggc caccatggcg tacaaagcca
     5941 aagacaacaa ttccaaaccc acatacaagg tcaagaagtg gttggcggaa accattacct
     6001 gcatgcccaa caaggcaaag aaggtcaaag tgtaaaattc acctttgaag atttcacggt
     6061 tggccaagta acgacggctg tacacaaaca gtacaaacac gcccgcatac atggccaatt
     6121 tcgccaattg tgaaatgcca tcattgacaa acaaattacc aaaagctgct tctggttgac
     6181 cgtgccaaac catcacttga gtagcggcca ccacaacgat gcccaccaag ctcaagacat
     6241 aggttaccca acggttgcgg tcgtttagaa acaagtctat caatagcacc aagcccaata
     6301 cgcataacag agtgatttct ggtatggcgg gggttaaatt taattctgcc caattcattc
     6361 aatacgtcct taaattttgc tttgtgcgac ttgttcaatt aaggtatttg ctgcttgatg
     6421 cacaaccgca ataaacggtt ctggatacaa acccataccc aatacagcca cggtcaaaat
     6481 gatcaaaatc aacaactcgc gtccattgac atcttttaat tggcgtactt cttcgttggc
     6541 aattgcacca aagatcacac gtttgtacat ccacaatgtg taagcagcac caaaaatcaa
     6601 tgttgacgca gccaacatac ccacccagaa gttatattca gtcgcgccca tgatgaccat
     6661 gaattcgccg atgaaacctg aagttgcagg caaacctgca ttggccatgg caaacaacat
     6721 catgaaagca gcaaacttag gcatggtatt caccacacca ccgtaatcgg cgatgttacg
     6781 tgtgtgcatg cggtcgtaca tcacaccgat acacataaac atggcagcag acacgaaacc
     6841 gtgtgaaatc atttgcatga tggcgccttt taagccccag tcattcaaag taccgtcaac
     6901 gaacatgaac aaacccaaag tcacaaaacc catgtggctg atcgaagaat acgccaccag
     6961 ttttttcatg tcggtttgaa ccaaagccac catacccaca tacacaaccg ctaccaaaga
     7021 caacactata accactggcg cgaagaaatg agaggcatct ggcacgatag gcaagatgaa
     7081 acgcaagaaa ccgtatgcac cga
//
LOCUS       DAOMVK010000059         7057 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_2839_length_7057_cov_3.437304, whole genome shotgun sequence.
ACCESSION   DAOMVK010000059 DAOMVK010000000
VERSION     DAOMVK010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 7057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7057
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2839_length_7057_cov_3.437304"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..767)
                     /locus_tag="PK856_03300"
     CDS             complement(168..767)
                     /locus_tag="PK856_03300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRL01432.1"
                     /translation="MNLPCLVHIVDDEAAVRESCAFLMEALGHAHQTWEHGQAFLDQA
                     DCYRPAVALIDLRMPFVTGEEVVQQLQQRHSCIAPLILTGHGDVATAVSLLKQGVVDF
                     LQKPVQVEPLQAAINQAWQHALKLQQLHVWRQCELSLTPKERHIVQLMVQGMTNKDMA
                     EALHVSQRTIEVHRANAMAKFQCEHLAQLVQIYFLLHPQ"
     gene            complement(764..>1308)
                     /locus_tag="PK856_03305"
     CDS             complement(764..>1308)
                     /locus_tag="PK856_03305"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRL01433.1"
                     /translation="LQHNVPNTMDIQAVLNRIVEQIDTSRHIIHNIRTWGKTLPAQPS
                     ERVPLLQVLTEVQQFVAWQHSQLPLLLQCEPDIHIDTEPLKLKQILVNCILNSIQAGA
                     TEVRLEALTGRLNIIDNGKGFAQAQLDFPFVPFRTGRNDGLGIGLVVCERLARSMDMR
                     MTLGNHSDGGAMVVLSWGAA"
     assembly_gap    1309..1318
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1319..2466)
                     /locus_tag="PK856_03310"
     CDS             complement(<1319..2466)
                     /locus_tag="PK856_03310"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HRL01434.1"
                     /translation="MNWSHFIHILLIYICLGLAPVQAATWLIGIQAPNGAAATQTQWQ
                     PWLDWLSAQFPDDDFTLVALNTEDFKNALQSHKLDFVIAQPVQLLMLPQQMPTHWLAS
                     VEHSTAYEISGDEVGSAVWVRQDSPLRQLSDLKKKRVAAVSEHAFGGHLAAMYVLQQH
                     GVRENDLEVVFQEYPVANALFALQQGQVDAAIAPLCLMEQLQRTGRMDAQQFRLLAPT
                     IHSAHCQGNVEFASNWLLAAMPDTPTELSKQVASVVLSAHTPPELPQWQSPQSTAAIE
                     QVLLQMGHHPYQQNLWQTMGFYAEKYRYWWLAAAVVLLLMLLNHALAVYLARRRQAQL
                     HQMYHNLAQTEQMMYHADRVHTLGEMMSGIGHELNQPLTAIQYYADGLR"
     gene            2576..3913
                     /locus_tag="PK856_03315"
     CDS             2576..3913
                     /locus_tag="PK856_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRL01435.1"
                     /translation="MKKIYIAFSGLVLAGLAAYGGSHAIMSSRTQSVQPKLKANDEVM
                     ARGEYVARSSDCFACHSVPGGQAYAGGLAMQTPVGAIYSTNITPDPETGIGKYSLAQF
                     TNAVKHGVRDDGAPLYPAMPYPSYTIMPDEDVEAMYAFFMHKVKPVKQANQASTMPWV
                     MSMRWPVAYWQGLFAPAREFVANPQWDTSTNRGAYLVEGPGHCGACHTPRGVAYQEQS
                     LSMADNDSFLSGAVVDGWRAKSLRGEGNGLGEWSENDIAQFLKTGRTDKIIAFGAMAE
                     VVEHSTQYMSAEDLGSMAKYLKALPPVAGRPVQRATQTDTTTQALLNGSDVSRGALLY
                     TENCTVCHRADGKGVARIFPALDDNSAVVANNAQSVIQVTLEGGRMAHTSSDAMAFTM
                     PAFKHLSDEDVKDIVNFVRTSWTNQAPTIATQDVTEIRRFLEKKSPHYVGEQP"
     gene            3910..4806
                     /locus_tag="PK856_03320"
     CDS             3910..4806
                     /locus_tag="PK856_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRL01436.1"
                     /translation="MKPLSKQLLWASVPVALALAACTGQPEAAKKNHYEVKVGKEVVK
                     TDYVVPGPTDVLGHENADLIVYGGRLLNDTKRLLPNNVGASMNCNSCHIANGKEQKGA
                     PYINSYFTYPKKMPRAGKVVDLQMRINGCFQRSMNGKPLDVNSKEMHAMIAYMAWLSE
                     GLPKGADIQMDVGGKINESLVPNPERGKQLYAQQCVTCHGDNGQGLKDARGNNVFPPL
                     WGDESFNIGAGMARTYKAAAFIRDNMPMSVQKNGAWGQGGVLSDQDAVDIAEYFTHQP
                     RPDFAGKVKDWPEGKKPKDARY"
     gene            5052..5564
                     /locus_tag="PK856_03325"
     CDS             5052..5564
                     /locus_tag="PK856_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01437.1"
                     /translation="MSDSDIDRKEVKEMSEEVQADLNADPITGEPGSHPVETGIAGTG
                     GAVIGAAIGAIGGPLGMLIGGAIGAMAGGLAGSAAGESIDPTKEDAYWREHFQEESYY
                     QAGYDYDTDYQPAYAVGYAMKSKYEQDTPFEHIEAELQQKWEEVKGTSRLTWLEAKDA
                     IRDGWNRVSN"
     gene            complement(5637..6806)
                     /locus_tag="PK856_03330"
     CDS             complement(5637..6806)
                     /locus_tag="PK856_03330"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRL01438.1"
                     /translation="MNYSNLKDIDVAGKTVLIRVDMNVPLNDGVITDDTRIRASLPSI
                     EYCLERGAGVVLMVHLGRPKEGILKPEDNVLPIAKRLSELLDQNIEIADNWRDVKPTP
                     KAGQVVMLQNVRVNVGEKANDPELGQAYAALCDVFVFDAFGAAHRAQASTHAVAQFAP
                     VACAGLLLSAELDALSQATEHPKPPVVAIVGGSKVSTKLTILESLSQKVDQLIVGGGI
                     ANTFLMAKGHNVGKSLAEADLVEEAKKIIASIEAKGGSVPLPTDVVVATSFSTDAEAV
                     IKDISEVGDDDMILDIGPNSQKVLADIIAGAGTVVWNGPVGVFEFDQFASGSKALAEA
                     IANSDAFSIAGGGDTLAAIAKFGVTEQISYISTGGGAFLEFLEGKELPAVAVLQK"
     gene            complement(6884..>7057)
                     /locus_tag="PK856_03335"
     CDS             complement(6884..>7057)
                     /locus_tag="PK856_03335"
                     /inference="COORDINATES: protein motif:HMM:NF012964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HRL01439.1"
                     /translation="GFSADCLETLEEIAISGKALFESAGGEQFDYIPCLNTEAVWTEA
                     LCLMVAQELSGWI"
BASE COUNT         1719 a         1723 c         1935 g         1670 t
ORIGIN      
        1 gtgatttttg acggtgagag cgatggcgaa gcggtgtatt tcaaaaacac tgaagccgta
       61 gacagctatt tgtcacctgc tgcggcaagc accactgcat aaattggttt gttttgaata
      121 agcggcctgt aaacgtttac aggccgctta tttgtgttta tcacacatta ttgaggatgc
      181 aataggaaat agatttgcac caattgtgcc aaatgctcgc attgaaattt ggccatggca
      241 ttggcgcggt gaacctctat tgtgcgttgt gacacatgca aggcttcggc catgtctttg
      301 ttggtcatgc cttgcaccat caattgcacg atatggcgtt ctttgggagt gaggctgagc
      361 tcacattgtc gccatacatg cagttgctgt aacttcagcg catgttgcca tgcttggttg
      421 atggcggcct gtaaaggctc aacctgaacg ggtttctgta gaaaatcgac cacgccttgt
      481 ttgagcagag acactgccgt tgccacgtca ccatgacccg ttaaaatcaa tggggcgatg
      541 cagctgtgcc gttgttgcaa ttgctgtaca acctcttcac cagtcacaaa cggcatgcgc
      601 aaatcaatca aggccacggc agggcggtag caatcggctt ggtctaaaaa tgcttgccca
      661 tgttcccaag tttggtgagc gtggcccaag gcctccatta agaaggcgca gctttcacgc
      721 accgcggctt catcgtcaac aatgtgtacc aagcaaggca ggttcatgct gctccccaac
      781 tcaataccac catggcaccg ccatcgctgt gattgcccaa agtcatgcgc atgtccatgc
      841 tgcgggcaag gcgctcgcac accaccaaac ctatgcccaa gccatcgttg cggccagtgc
      901 gaaacggcac aaagggaaag tccaattgcg cttgtgcaaa gcctttgcca ttgtcgatga
      961 tgttcaagcg gcctgtaagt gcttccaagc ggacttcagt cgcccctgct tggatgctgt
     1021 tcaaaatgca attcaccaga atttgtttga gtttcaaggg ttcggtgtcg atgtgtatgt
     1081 cgggctcaca ctgtagcaga agcggcaatt gactgtgttg ccatgcgaca aattgttgga
     1141 cttcggtcaa tacctgcaat aagggcacgc gctcactggg ctgagcaggc aaggttttac
     1201 cccaagtgcg gatgttgtga atgatgtggc gactggtgtc gatttgctcg acgatgcggt
     1261 tgagcacggc ttgaatgtcc atggtgttgg gcacgttgtg ttgcaattnn nnnnnnnncg
     1321 caagccatcg gcatagtatt ggatggcagt cagtggctgg ttcaactcat ggcctatgcc
     1381 tgacatcatt tctcccaaag tatgcacgcg gtcggcgtgg tacatcattt gttcggtttg
     1441 ggccaagttg tggtacatct ggtgcagctg tgcttgtctg cgtctggcca aatacacggc
     1501 caaggcgtgg ttgagcaaca tcaacagcaa taccacagcc gctgcaagcc accaatagcg
     1561 gtatttttcg gcataaaaac ccatggtttg ccacaggttt tgttggtagg gatggtggcc
     1621 catttgcagc aagacttgct ctatggctgc tgtggactgc gggctttgcc attgtggcaa
     1681 ttcaggcggg gtgtgagcag acaagaccac actggccact tgtttgctca gctcggtggg
     1741 ggtgtcgggc atggccgcca gcaaccaatt ggaagcaaat tcaacattgc cttggcaatg
     1801 ggcgctgtgg atggtggggg caagcaatcg aaactgttgg gcatccatgc ggcctgtacg
     1861 ctgcaattgt tccatcaagc acaacggcgc gatggcggca tccacttggc cttgctgcaa
     1921 ggcaaataaa gcattggcga cggggtattc ttgaaacacc acctcaaggt cgttttcacg
     1981 cacaccgtgt tgttgcaaca catacatcgc tgccaaatgg ccaccaaaag catgttctga
     2041 cactgcggcc acacgctttt ttttcaaatc actgagctgt ctgagcgggc tgtcttgtcg
     2101 cacccacaca gcactgccga cttcgtcgcc actgatttca tacgcagtgc tgtgttccac
     2161 gctggcaagc caatgggtgg gcatttgctg cggcaacatc agcaattgca cgggctgggc
     2221 gatgacaaaa tccagtttgt ggctttgtaa ggcatttttg aagtcttcgg tgtttaaggc
     2281 cactagggtg aaatcatcgt ctggaaactg ggcagagagc caatccagcc aaggttgcca
     2341 ttgggtttgg gtggcggcgg cgccattggg ggcttggatg cctatcaacc aagtggcggc
     2401 ttgaacgggg gcgaggccca aacaaatgta aatcagcaag atgtgaatga aatgtgacca
     2461 attcataaag ttttcctgct tgatgtaagt caacaagcat gtgggtatgt ggttttccac
     2521 aatatacagc agcggcctgt attgggaaca tggtgccatg aacaaggaaa cagccatgaa
     2581 aaaaatctac attgcattca gtggactggt gcttgccgga ttggccgctt atggtggctc
     2641 gcacgccatc atgtcatccc gaacccaaag tgtacagccc aaactcaagg ccaatgatga
     2701 ggtgatggcg cgaggggaat acgtggcgcg cagctcagat tgttttgctt gccacagcgt
     2761 gcctggtggc caagcatacg caggtggttt ggcgatgcaa acgcctgtgg gcgcgattta
     2821 ctccaccaac atcaccccag accctgaaac gggcataggc aaatacagct tggcacagtt
     2881 taccaatgcg gtgaaacacg gtgtacgaga tgatggcgcg cctttgtacc ccgccatgcc
     2941 ttatccatcg tataccatca tgcccgatga agacgtggaa gcgatgtatg cgtttttcat
     3001 gcacaaagtg aaacctgtga agcaggccaa tcaagcctct accatgccgt gggtcatgag
     3061 catgcgttgg cctgtggcgt attggcaagg cttgtttgca cctgcgcgtg agtttgttgc
     3121 caaccctcaa tgggatacca gcaccaaccg cggcgcgtat ttggtggaag ggcctgggca
     3181 ttgtggcgct tgtcataccc cgcgtggtgt tgcctatcaa gaacaatctt tgagcatggc
     3241 cgataacgac agcttcttgt caggcgcggt ggtagacggt tggcgcgcca agagtttgcg
     3301 tggtgagggc aatggtttgg gtgagtggag tgaaaacgac atcgctcaat tcctcaaaac
     3361 aggccgtacc gataaaatca tcgcttttgg tgcgatggcg gaagtggtgg agcacagcac
     3421 ccaatacatg agtgcagagg atttgggcag catggccaaa tacttgaaag ccttgcctcc
     3481 cgtagcaggg aggcctgtac agcgtgctac ccaaacagac accacgaccc aagccttgct
     3541 caatggcagc gacgtcagcc gtggcgcctt gttgtacacg gaaaactgta ctgtttgtca
     3601 ccgtgccgat ggcaaaggtg tggcgcgaat tttccctgcc ttagacgaca acagtgcggt
     3661 ggtggccaac aatgcccagt cggtgattca agtgactttg gaaggtggtc gcatggcaca
     3721 cacgtccagc gacgcgatgg ccttcaccat gcccgcattc aagcatttga gcgatgaaga
     3781 cgtgaaagac atcgtcaatt tcgtgcgcac cagttggacc aatcaagcgc ccaccatcgc
     3841 aacccaagac gtgaccgaaa ttcggcgctt tctggaaaag aaatcacccc attacgtggg
     3901 agaacaacca tgaaaccatt gagtaagcaa ttgttgtggg cgagtgtgcc tgtggcgttg
     3961 gccttggcgg cctgtactgg ccaacctgag gcagccaaaa aaaaccatta cgaggtcaaa
     4021 gtcggcaagg aagtggtgaa aaccgattat gtggtgccag gacctaccga tgtgttggga
     4081 catgaaaacg ccgatttgat agtctacggc ggccgcttgt tgaacgacac caaacgcttg
     4141 ttgcccaaca atgtcggtgc cagtatgaat tgtaacagct gccacattgc caatggcaaa
     4201 gagcaaaaag gggcgccgta catcaattcg tatttcacct atcccaagaa aatgccgcgg
     4261 gctggcaagg tggtggattt gcaaatgcgc atcaatggct gttttcagcg ttcgatgaat
     4321 ggcaagccac tggatgtaaa ttccaaagaa atgcacgcga tgattgccta catggcatgg
     4381 ttgagcgagg gtttgcccaa aggtgcagac atacaaatgg atgtgggagg caagataaac
     4441 gagagtctgg tgcccaatcc tgagcgcggc aaacagctgt atgcacagca atgtgtgact
     4501 tgccatgggg acaatggcca aggtttgaaa gacgcacggg gcaacaatgt gttcccaccg
     4561 ctgtggggcg atgagtcatt caacatcggt gcaggcatgg cgcgcacata caaagccgcg
     4621 gccttcattc gcgacaacat gccgatgtcg gtgcaaaaaa atggcgcttg ggggcagggc
     4681 ggcgtgttga gcgaccaaga tgcggtcgac attgccgagt atttcaccca ccaaccgcgg
     4741 cctgattttg ccggcaaagt gaaagactgg cctgaaggta aaaagcctaa agacgcgcgt
     4801 tattgagtat taatatccat gtagggtttg aggccgtgtt tacacggctt tttttgatgt
     4861 ataagcggcc tgtaaatgat tacaggctgc tttttacatg tcattaaaaa ccatggaata
     4921 gacttaaaaa tcaaacatta aaatgggtaa aaatgcgaat ttctcacaca gcttgggaaa
     4981 caaaacccac cactcgacct cagagcaaaa cggttcacag ccgtgacgtt tcacatcata
     5041 aggagaatac catgtcggat tctgacattg accgcaaaga agtgaaagaa atgtccgagg
     5101 aagtacaagc agacttaaac gccgacccga ttacgggtga gccaggttcc catcctgtgg
     5161 aaacagggat agctggcacg ggaggggccg tcattggagc ggccatcggt gccataggcg
     5221 gcccgttggg catgttgata ggtggggcaa ttggggcgat ggccggtggt ttggcaggca
     5281 gcgctgcggg tgaatccata gacccaacca aggaagacgc ttattggcgt gagcatttcc
     5341 aagaagaaag ctattaccaa gcgggttatg attacgacac cgattaccaa cccgcttacg
     5401 ccgtgggtta tgcgatgaaa tcaaaatatg aacaagacac gccttttgaa cacattgaag
     5461 cagaattgca gcaaaagtgg gaagaagtca aaggcacatc ccgtttgact tggttggaag
     5521 ccaaagacgc gatacgcgat ggttggaatc gggtttccaa ctgatgatga ttattcaaac
     5581 atgtgacaaa aaagcggcct gtaaacattt acaggccgct ttttatgagc aaaggcttat
     5641 ttttgcaaca ctgccactgc gggtaattct ttgccttcta agaattctaa gaaagcgccg
     5701 ccgccagtag agatgtagct gatttgttca gtcacgccga atttggcaat ggctgccaag
     5761 gtgtcgccgc cgcctgcaat ggagaaggca tcgctgttgg cgatggcttc ggccaaggct
     5821 ttgctgcctg atgcgaattg gtcaaattca aacacgccca ctgggccatt ccacaccacg
     5881 gtgcctgcgc cagcgatgat gtcggccaat actttttgtg aattggggcc gatgtccaaa
     5941 atcatgtcgt cgtcgcccac ttcgctgatg tctttaatca ccgcttcggc gtcggtgctg
     6001 aatgatgtcg ccaccaccac atcggtaggc aaaggcactg agccgccttt ggcttcaatg
     6061 gaagcgatga tttttttggc ttcttccacc aagtcggctt ctgccaaaga tttgcccacg
     6121 ttgtggcctt tggccatcaa gaaggtgttg gcaatgccgc cgcccacaat caattggtcc
     6181 actttttggc tcaaactttc caaaatggtg agcttggtag acactttgct gccgccaacg
     6241 atggcgacta cgggtggttt tgggtgttcg gtagcttggc ttaaggcatc gagttcggca
     6301 gataacaaca agcctgcgca ggccacaggg gcaaattgcg ccacagcatg agtagaggct
     6361 tgcgcacggt gggccgcgcc gaaggcatca aacacaaaca cgtcgcacaa ggcggcatag
     6421 gcttggccca attctgggtc attggctttt tcgcccacgt tcacgcgcac gttttgcaac
     6481 atcaccactt ggcctgcttt gggagtgggt ttgacatcgc gccagttgtc ggcgatttca
     6541 atgttttggt cgagcaattc gctcaaacgc ttggctatcg gtaacacatt gtcttcaggt
     6601 ttcaagatgc cttctttagg gcgacccaaa tgcaccatca ataccacgcc tgcgccgcgt
     6661 tctaaacagt attcgatgct gggcaaagaa gcgcggatgc gggtgtcgtc ggtaatcacg
     6721 ccatcgttca gtggcacgtt catgtcaacg cggatgagga cggttttgcc cgctacgtcg
     6781 atgtctttga ggttggaata gttcattgtt ttgatttctg agactaaaaa ggtataaaag
     6841 ctgaattatc ctatatcggc ttggttttcg ccaagtcctt gttttaaatc cagcccgata
     6901 attcttgcgc caccatcagg cacaaggctt cggtccacac tgcttcggta ttgaggcagg
     6961 gaatgtaatc aaattgctca ccgccagcgc tttcaaacag cgctttgccg ctgatggcga
     7021 tttcttccaa ggtttccaaa caatcggcgc tgaaacc
//
LOCUS       DAOMVK010000060         6989 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_2889_length_6989_cov_3.087395, whole genome shotgun sequence.
ACCESSION   DAOMVK010000060 DAOMVK010000000
VERSION     DAOMVK010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 6989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6989
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2889_length_6989_cov_3.087395"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PK856_03340"
     CDS             <1..243
                     /locus_tag="PK856_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated protein Cas7f/Csy3"
                     /protein_id="HRL01440.1"
                     /translation="LRSIDTWYPEFDTAQMAIAIEPYGAVTNLGKAYRTPKDKVDFYT
                     LFDQFAVGKTLDTVEQEHYVMAVLVRGGVFGQSGKD"
     gene            247..834
                     /gene="cas6f"
                     /locus_tag="PK856_03345"
     CDS             247..834
                     /gene="cas6f"
                     /locus_tag="PK856_03345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated endoribonuclease
                     Cas6/Csy4"
                     /protein_id="HRL01441.1"
                     /translation="MSHYQELTLLQHEEVPLFALWGKVYVQIHLALTELQAALGTGKV
                     GVAFPEYRFDAAVQMGRLGSKLRVFADDAAILQQLNLSQHLRRYADYVQLSAVAAVPA
                     EHGLACYARVQVKSGAERATRRLIKRHPELNYETELARLQALNAHSALPFIHMNSLSS
                     KQAFRLFIAKSEATVVQTGDFGTYGLSNSATVPDF"
     gene            1097..1432
                     /locus_tag="PK856_03350"
     CDS             1097..1432
                     /locus_tag="PK856_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01442.1"
                     /translation="MKKTMLMAMCLALAACSGQSVEQVVKKVQDKTIKDGDAIVVTGV
                     VGKFGAEPEPFVLLQSADKLNAVYGILPKEALAGLSEGKQIRLDCRSIHFQNNGKGML
                     VTSRNCVVK"
     gene            complement(1507..2925)
                     /gene="glnA"
                     /locus_tag="PK856_03355"
     CDS             complement(1507..2925)
                     /gene="glnA"
                     /locus_tag="PK856_03355"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HRL01443.1"
                     /translation="MSVQNVLDLIEENDVKFVDLRFTDTKGKQQHVSIPAHVVLADGE
                     EWFEEGQAFDGSSIGGWKGIQASDMTLRADPTTAFIDPFYDQPTVVLTCDVIDPANGQ
                     GYDRDPRSIAKRAEAYLKSSGIGDTAYFGPEPEFFIFDGIEWETTMQGTRYKIHSEEA
                     AWSSGESYDGQNTGHRPFVKGGYFPVAPVDSGQDLRSAMCLTLEALGIPVEVHHHEVA
                     TAGQMEIGTKFNTLVRRADWTQDMKYVIQNVAHNYGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDMALYYIGGIIKHAKALNAITNPSTNSYRRLVPHYEAPT
                     KLAYSAKNRSASIRIPHVASSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCADLDEALEALKADHEFLTRGGVFSKDWIDSYIAFK
                     ADDVARIRMAPHPLEFEMYYSL"
     gene            3174..4001
                     /gene="aroE"
                     /locus_tag="PK856_03360"
     CDS             3174..4001
                     /gene="aroE"
                     /locus_tag="PK856_03360"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRL01444.1"
                     /translation="MQPVARYAVFGHPIAHSRSPEIHLAFAKQEGVDIVYEKMLLPLD
                     GFATGVRAQLELGLQGANVTVPFKLDACAFADELSERADKAGAANTLIVRADGSVLGD
                     NSDGLGLMQDITVNQGMTLRNKKVLLLGAGGAVRGVLQPLLALQPGSVCVANRTLSKA
                     QDLADVFEIDALDLQAASQQHYDIIINGTSTGLSNVAPNLAAHSFAQCELAYDMVYGA
                     EPTPFMRMAAAAGAKSTTDGLGMLVGQAAVSYQWWRGFQPDTQTVLAAMRASLCSNH"
     gene            3986..4747
                     /gene="mtgA"
                     /locus_tag="PK856_03365"
     CDS             3986..4747
                     /gene="mtgA"
                     /locus_tag="PK856_03365"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HRL01445.1"
                     /translation="MFKSLKALIACVLGLVLVFQCYAYGSILFWRAFAPESTSFMRTR
                     AMQYSDESVKYDWVAYGNISNNLKQAVIASEDAAFTNHDGFDWRGIENAMKRNQRAGE
                     IKAGGSTISQQLAKNLFLWEGKSYVRKAEEAAITLMMEATTDKERIFEIYLNVIEWGP
                     NVFGAEAAAQHHFDKSADKLTKQQAAKLAAMIPRPRYFSENPKDRNLRGKTNIILRRM
                     GASDLPESDPMFQMPNLGALTQPQEEPVSKHKRKP"
     gene            4804..5220
                     /locus_tag="PK856_03370"
     CDS             4804..5220
                     /locus_tag="PK856_03370"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HRL01446.1"
                     /translation="MAEECELTDGLRRRLIQLECQLHHVERNNPVVLNQCLHTDFKEF
                     TQSGRVLNKHQMVAALLAEQDADKIYAQDFQLQVLSNHSALLTYLSHQVHAQTQAQYN
                     WALCSSIWHRNEQDVWQLLFHQGTPCAKPTVPVTGC"
     gene            5419..6597
                     /locus_tag="PK856_03375"
     CDS             5419..6597
                     /locus_tag="PK856_03375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HRL01447.1"
                     /translation="MVAAYENSIRDRRLKRIYNLHDFECRARKLLPRALAEYVFGGVE
                     DNVTLANNRRQFDDHSLLTGVLRDVSQRHTQHTVFGKSYAAPFGIAPIGVSALIAFNG
                     DNVLASAAYNLGIPMILSGSSLTRLEEVKQHHPNVWFQAYLPGDADLQAALIERVAAA
                     GIDTLVITVDIPVWANRENNMRAGFSLPLRPSLSLMVDGLLHPRWSLGTFWATLQSKG
                     MPHFENSFATRGAPVFSSAAVRDTTGRDHINWAHIKRIRRLWSGKLILKGVLSVADAR
                     LSQQYGAVGIIVSNHGGRQLDTVISPLQQLPLIRQAVGPDYVVMMDSGIRRGTDVIKA
                     LALGADFVFTGRPFISAAAVAGVSGVEHAITLLQQEVERNMGMLGITQLADINRECLA
                     "
     gene            6608..6841
                     /locus_tag="PK856_03380"
     CDS             6608..6841
                     /locus_tag="PK856_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01448.1"
                     /translation="MWKKLGNWLGTALIIVMLPAMLLQGVGEFLIYGMGYWPRRLFNR
                     KAKLTDDINAWLGILYWFLLVAAAFAYYSGGHS"
BASE COUNT         1675 a         1569 c         1935 g         1810 t
ORIGIN      
        1 ctgcgcagca tagacacttg gtatcctgaa tttgacacgg cacaaatggc gattgcgatt
       61 gagccttatg gcgcagtcac aaatttgggc aaggcttacc gcacgcccaa agacaaagtg
      121 gatttttaca ccttgtttga ccaatttgca gtgggcaaaa ccttggacac ggttgaacaa
      181 gagcattatg tgatggcggt gttggtgcgc ggtggtgtgt ttggacaaag tggcaaggat
      241 tagtctatga gccattatca agagctaacg ctgttgcagc atgaagaagt gccgctgttc
      301 gcgctgtggg gcaaggtgta tgtgcagatt catttggctt tgaccgagtt acaggccgca
      361 ttgggcacag gcaaagtggg tgtggcgttt cctgaatacc gttttgatgc cgcggtgcaa
      421 atgggtcgct tgggcagtaa attgcgggtg tttgcagacg atgcggccat tttgcagcaa
      481 ttgaatctgt cgcagcattt gcgccgctat gcggactatg tgcaattgag cgcagtggcg
      541 gccgtgcctg ccgagcatgg gttggcgtgt tacgcgcgtg tgcaggtgaa aagtggtgct
      601 gagcgggcga cgcggcgttt gattaagcgc catcctgagc tgaattatga aacagaattg
      661 gcgcgcttgc aggctttgaa tgcacacagc gccttgccgt ttattcacat gaacagcttg
      721 agcagcaagc aggcttttcg tttgtttatt gcaaagtcag aagcaacagt ggtgcagact
      781 ggcgattttg gcacctacgg cctcagcaac agtgccaccg tgccagattt ttaaggtaaa
      841 tgcgtgtatg aaaaacctcc tgcgggaggt ttttttatgg caggcaatgt ggcagggctc
      901 gatgtgtgta aacctagtac tgatgaaact gttgggtgtg tttaagcggt ctgtaaggtt
      961 taataatagt tttgggcgaa ttaaaagtga gtgtgtgttt gtgcatcgac taggcagtgt
     1021 ttgtctgtac agaaagcgct tacaggccgc tcttgggcaa tgttaaaatg ggccattcaa
     1081 tcaaatgggt ttgggcatga aaaaaacgat gttgatggcc atgtgcttgg cattggcggc
     1141 atgcagtggg caatcggttg agcaagtggt gaaaaaagtg caagacaaaa cgattaaaga
     1201 cggtgatgcg attgtggtga cgggagtggt gggcaagttt ggcgccgagc ctgagccatt
     1261 tgtgttgctg caaagcgcag acaaactaaa cgcggtgtat ggcatcttgc cgaaagaggc
     1321 tttggctggc ttgagtgaag gtaagcagat tcgtttggat tgtcgcagca tacacttcca
     1381 aaacaatggc aaagggatgt tggtcacgtc gcgcaattgt gtggtgaaat agcggtctgt
     1441 aaagctgctg caaaagaaaa aagcctgacc caatggtcag gcttttgtat atgcttacag
     1501 cagatattaa aggctgtaat acatttcaaa ctcaagcgga tgaggtgcca tgcggatgcg
     1561 ggcaacatcg tctgctttga atgcgatgta gctgtcgatc cagtctttgc tgaacacgcc
     1621 accgcgagtc aagaactcgt ggtcggcttt caatgcttcc aaagcttcat ccaagtcggc
     1681 gcatacggtt ggcaccaatg cgtcttcttc aggaggcaag tcgtacaggt ttttgtctgc
     1741 tggatcgcct gggtggattt tgttttggat gccgtccaag ccagccatca acaaggcagc
     1801 aaaggccaag tatgggttag cggtagggtc tgggaaacgt gcttcaatgc ggcgggcttt
     1861 tgagctggca acgtgtggaa tgcgaatcga agcagagcgg ttcttcgctg agtaagccag
     1921 tttggttggc gcttcgtaat gaggcaccaa acggcggtat gagttggtag acggattggt
     1981 aatcgcgttc aaggctttgg cgtgcttgat gatgccgccg atgtaataca aagccatgtc
     2041 gctcaaacct gcgtaaccgt cacctgcaaa caggttttgg ccgtctttcc aaatcgattg
     2101 gtgcacgtgc atgccagagc cattgtcgcc catgatcggt ttaggcatga aagtggcggt
     2161 tttgccgtag ttgtgagcca cgttttggat cacgtatttc atgtcttggg tccagtcggc
     2221 gcgacgaacc aaggtgttga atttggtgcc aatttccatt tgacctgcag ttgccacttc
     2281 gtggtggtga acctccacag gaatgcccaa ggcttccaaa gtcaaacaca tggcagagcg
     2341 caagtcttgt ccagaatcca cgggggcaac tgggaagtag ccgcctttaa cgaatgggcg
     2401 gtggcctgtg ttttgaccgt cgtagctttc gccgctagac caagccgctt cttcagaatg
     2461 gattttgtag cgggtgcctt gcatggtggt ttcccactcg atgccgtcaa aaataaagaa
     2521 ttctggttct gggccaaagt aagcggtgtc accgatgcct gaagatttca aataggcttc
     2581 tgcacgtttg gcgatagagc gtgggtcgcg gtcgtaacct tgaccattgg caggatcaat
     2641 cacatcacac gtcaacacca ctgttggttg gtcgtagaat gggtcgatga atgcagtcgt
     2701 agggtcggca cgtaaagtca tgtctgacgc ttgaatgcct ttccaaccac caatagaaga
     2761 accgtcaaac gcttgacctt cttcaaacca ttcttcacca tccgccaaca caacatgggc
     2821 agggatgctc acgtgttgct gtttgccctt ggtatcggta aaacgcaagt cgacaaattt
     2881 gacgtcattt tcttcaatga ggtccaatac attttggaca gacatagtgc agctccttta
     2941 aagcaatgcg aagtgtggga tataaattca atgcaagtgc cttgattgtg ccacactttt
     3001 tttatgaaac aaatgtcgca atgcaaaaaa aagagcaaat tctgacaaat ggctgagttc
     3061 gttgtaaaaa tattttcata ttatcattta tactcaaatt ggaactattt tgcacattca
     3121 ttaaaatggg cttaatatgg ctgccaactt gatttgacat agattttacc gccatgcagc
     3181 cagtcgctcg ttatgccgta tttggccatc ccattgccca cagtcgttcg ccagaaatcc
     3241 atttggcttt cgccaagcaa gagggggtgg acatcgttta tgaaaaaatg ctgttgccat
     3301 tagatggctt tgctacaggt gtgcgtgcac agcttgaatt gggcttgcaa ggggcgaatg
     3361 tgaccgtgcc tttcaagctt gatgcgtgtg cgtttgccga tgaattgagc gaacgtgctg
     3421 ataaagcagg cgcggcgaac actttgattg tgcgtgcaga cggcagcgtg ttgggcgaca
     3481 attccgatgg cttgggtttg atgcaagaca tcacagtcaa tcaaggcatg actttgcgca
     3541 ataaaaaagt attgttgttg ggagctggtg gcgcggtgcg tggggtattg caacctttgt
     3601 tggctttgca acccggcagt gtgtgtgttg ccaatcgtac cttaagcaag gcacaagact
     3661 tggccgatgt gtttgagatt gatgccttgg atttacaggc cgcatctcag caacattacg
     3721 acattatcat caatggcact tcaaccggcc tgagcaatgt cgctcccaat ttggcggcgc
     3781 acagctttgc gcagtgtgaa ctggcttacg acatggtgta tggagcagag cccacacctt
     3841 tcatgcgcat ggccgctgcg gctggagcga agtccaccac cgatggtttg ggcatgttgg
     3901 tgggacaagc ggcagtgtct tatcaatggt ggcgtggatt tcaacctgac acgcaaaccg
     3961 tcttagcagc tatgcgagcc agtctatgtt caaatcatta aaagccttaa tcgcctgtgt
     4021 attgggtttg gtgctggtgt tccagtgcta tgcctatggc agcatcttgt tttggcgcgc
     4081 gttcgcgcct gaatcgacca gtttcatgcg cacgcgggcc atgcaataca gcgatgagtc
     4141 tgtcaaatac gattgggtgg cctatggcaa catcagcaac aatctgaaac aagccgtcat
     4201 cgcttctgaa gacgctgcgt ttaccaatca cgatggtttt gattggcgtg gcattgagaa
     4261 cgcgatgaag cgcaaccagc gtgcggggga aatcaaagca ggcggctcaa ccatttccca
     4321 gcaattggcc aagaatctgt ttttgtggga aggcaaatcc tatgtacgca aggccgaaga
     4381 agccgccatt accttgatga tggaagccac caccgataaa gagcgcattt ttgagattta
     4441 cctgaacgtg attgaatggg gtccaaatgt gtttggtgcc gaagcggcgg cgcaacacca
     4501 ttttgacaaa tctgccgaca aattgaccaa gcagcaagcg gccaaattgg cagcgatgat
     4561 accgcgccca cgttatttca gtgaaaaccc gaaagaccgc aatttgcgtg gcaaaaccaa
     4621 catcatcttg cgccgtatgg gggcttctga tttgcctgag agcgacccca tgttccaaat
     4681 gcccaatttg ggtgctttga cccagcctca ggaagagcct gtgtctaaac acaagcgcaa
     4741 accataatct ggcgatgcgt tctgtcagcc acaagtttgg ttgggtttta ttaaggacag
     4801 ggtatggccg aggagtgtga actcacagat ggtttaagac ggcgattgat tcagctcgaa
     4861 tgccaactgc accatgttga gcggaacaat ccagttgtac tgaatcaatg cttgcacacg
     4921 gatttcaaag aattcaccca gtcaggccgt gtgctcaaca agcatcaaat ggtggcggca
     4981 ctgctggctg agcaagatgc agacaagatt tatgcccaag actttcaatt gcaggtttta
     5041 tccaatcaca gcgccttact cacctacctg agccatcaag tccatgctca aactcaggcc
     5101 caatacaatt gggctttgtg ctcgtccatt tggcacagga atgagcaaga cgtttggcaa
     5161 ttgctgtttc accaaggtac gccttgcgcc aaaccgactg tgcctgtgac tggctgctga
     5221 aatcgacatc atctgcttta tttaatttcc atcgaccctg attcaggaaa catgaaagaa
     5281 gcaggttcaa atgaaacagt gcagtatgat tgtttgcgag ccttgccatc tgaacgacaa
     5341 aacttgcttg attccattct tttggcatag catggttgcc actgattctg gtctttaacc
     5401 cctaaggagt gttgcctgat ggtggcggct tatgaaaatt ccattcgcga taggcgcttg
     5461 aaacgcattt acaatttgca cgattttgaa tgtcgcgctc gcaagttgct gccacgggct
     5521 ttggccgaat atgtgtttgg gggagtggaa gacaatgtga ctttggccaa taaccgccgc
     5581 caatttgatg atcacagctt actcactggc gtactgcggg atgtgtctca acgccacacg
     5641 caacacactg tatttggaaa atcctatgcg gcaccttttg ggattgcgcc gataggcgtg
     5701 agtgccttga ttgcgtttaa tggcgacaat gtgttggcca gcgccgccta taatttgggc
     5761 atacccatga tcttgagcgg cagttcttta acacgcttgg aagaagtgaa acaacaccat
     5821 cccaatgtgt ggtttcaagc ctatttgcct ggcgatgccg atttacaggc cgcattgata
     5881 gagcgcgtgg cggctgcggg catagacacc ttagtaatta ccgtggacat ccctgtgtgg
     5941 gcgaaccgtg aaaacaatat gcgcgcaggc ttttctttgc cgctgcgccc gtccttgtct
     6001 ttgatggtgg atggcttact gcacccgcgt tggagtttgg gcacgttttg ggccacgctg
     6061 caaagcaaag gcatgccgca ttttgaaaac tcgtttgcta cccgcggtgc accagtgttt
     6121 tcttcggcag cggtgcgcga caccaccggg cgggatcaca tcaattgggc gcatatcaag
     6181 cgcatacgca ggctttggtc aggaaaactc atcctcaaag gggtgttgtc cgtcgctgat
     6241 gcaaggctga gccaacaata cggtgccgtt ggcatcatcg tgtcgaatca tggtggccgt
     6301 caattggaca ctgtcatcag tccattgcag caattgcctt taatccgcca agcggtaggg
     6361 ccagattatg tggtgatgat ggattcaggc atacgtcgcg gcacggatgt gattaaggct
     6421 ttggccttgg gcgctgattt tgtgtttaca ggccgtcctt ttatttcggc ggcggctgtg
     6481 gctggcgtga gtggggtgga gcacgccatc accttgttgc agcaagaagt ggagcgcaac
     6541 atgggcatgt tgggcattac ccaacttgcc gacatcaatc gtgaatgttt ggcttgaatc
     6601 gaatcgtatg tggaaaaaat tgggcaattg gctgggtacg gcgctcatca tcgtgatgct
     6661 gcctgcaatg ttgctgcaag gtgtgggcga gtttttgatt tacggcatgg gctattggcc
     6721 acggcgtctg ttcaaccgca aagccaagct cactgacgac atcaatgctt ggttaggcat
     6781 cttgtattgg ttcttactgg tggcagcagc gtttgcttat tacagcggtg gacattccta
     6841 ataaagacat gaacaagcgg cctgtaaacg atttacaggc cgcttgtttg gtttgaatcc
     6901 tgattaaggt gcaaagctaa agaacaaata cgccgcaaaa atcaccaaat gcaccacgcc
     6961 tggcaagctg gtggtgcggc ctgtgcgca
//
LOCUS       DAOMVK010000061         6891 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_2955_length_6891_cov_2.469865, whole genome shotgun sequence.
ACCESSION   DAOMVK010000061 DAOMVK010000000
VERSION     DAOMVK010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 6891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6891
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2955_length_6891_cov_2.469865"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PK856_03385"
     CDS             <1..242
                     /locus_tag="PK856_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HRL01449.1"
                     /translation="AGDLLVVDKSLHAKQGDIVVADIGHEFTIKRLHLHGGMSLHPEN
                     HLENYPILYPQAEQEWRLVGVITYVIKSVHSSCMR"
     gene            230..1498
                     /locus_tag="PK856_03390"
     CDS             230..1498
                     /locus_tag="PK856_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HRL01450.1"
                     /translation="MYALIDGNHFYCSCERVFRPDLKSRPVVVLSNNDGCVVSRSPEA
                     KLLGIKMGVPYYQVAHLQKANELVVFSSNYPLYSHLSNHMMRSIASLVPHTEVYSIDE
                     CFADVTGLSDLAELATNIRERVLTWVGIPCCVGVAPTKTLAKLCNHLAKKHACFNSVV
                     VWGNWRSDIQQRALRSVDINEVWGIGRKFNQRLRLLNIHTAWDLCQADTASLRKQFGV
                     TLERTQRELQGIACLDLESQAPAKQSILCSRSFQSQVTRLDDLSAALTHHVQEATFKL
                     RHEQSHCHYLRVFAYSNRFREDLPQYYGHQGIHLPEPCDDTLWLQHLAQQQLRDLFQA
                     GISYKKCGVELAMLQKKHITQMDWIESTVTRKQRPELMQAWDAIKRKYGDKQLHLASE
                     DLGSEAWKMRQQFKSPNYTTNFKDILVIPN"
     gene            1775..2098
                     /locus_tag="PK856_03395"
     CDS             1775..2098
                     /locus_tag="PK856_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01451.1"
                     /translation="MNRFLIILMSTAIAACNAIPNTTLITVQKPLGTLQCQDNDNTEH
                     RLIQLSTQLKEAEIKIASAAISHDGRSRPTVCGAADGKIGLFKIPFKQLQQAQQLGYS
                     LRESD"
     gene            2481..3152
                     /locus_tag="PK856_03400"
     CDS             2481..3152
                     /locus_tag="PK856_03400"
                     /inference="COORDINATES: protein motif:HMM:NF025100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HRL01452.1"
                     /translation="MTATLHLKSLFHWSAQDDVPLPELAAIDAKQRRRLSSLAKLSLA
                     ARQALEQEVDLTLTDHILWCSSYGDEHKTLAIMQDIARDEAPSPTAFATSVHNAIAGL
                     YSMLYQDDVATTSMNVGHTEFGNALWYAHALLQTHAANQVLLVCADEPLPDVYAQQDL
                     QQAYAMTCVLCRPDEHHPANLHIGATEQRAQTQLLQAQAVRFAEFWQSNATTLHMDAW
                     QLCKR"
     gene            3140..3925
                     /locus_tag="PK856_03405"
     CDS             3140..3925
                     /locus_tag="PK856_03405"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRL01453.1"
                     /translation="MQTVDGRLAHYWRIIATGISFTVFGVGGLCMGLLAMPLLLLMCR
                     NREQRRLRAQYWIHRGFRWFIRLMQALGVMRLEVSGLDRLQANVPQLVLANHPSLLDV
                     VCLISIMPQADCIVKQALWRNPFMRGAVQAADYILNDQSEQLLHNSVNRLHGNNKMIV
                     FPEGTRTAKNTVLNPFQRGAAHIAIASEAEILPVLIRCTPSTLSKNEAWYHVPPQRFV
                     ISVDFQDSIDVATWRQIDAAPTIQVRRLNAFLHDYFLRKLGHE"
     gene            3918..4172
                     /locus_tag="PK856_03410"
     CDS             3918..4172
                     /locus_tag="PK856_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HRL01454.1"
                     /translation="MSDLHLEVKQMIIDVLALEDVSAADIDDAAPLFGDGLGLDSIDA
                     LELGLALKKRYNIKLNQDSAETKQHFSSVNALVALIQAQA"
     gene            4211..4453
                     /locus_tag="PK856_03415"
     CDS             4211..4453
                     /locus_tag="PK856_03415"
                     /inference="COORDINATES: protein motif:HMM:NF003757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRL01455.1"
                     /translation="MQTREDIFVALKQWMVDLFEIDPDKITMDAHLYEDLDIDSIDAV
                     DLVVKIREITGKQIQAEEFKHVRRISDVVDAIAKLN"
     gene            4468..4956
                     /locus_tag="PK856_03420"
     CDS             4468..4956
                     /locus_tag="PK856_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01456.1"
                     /translation="MQHSLPIKILLVVAFLCYPFLMLFGLSHWGITPIALVLMVATLL
                     KLLVSKNSQLKPLYVLGILCGLLSLLRQDEMWVKFYPACMNVGWLLVFAWSLHTDQSM
                     IERIARTHEPDLPLLVWHGHAKSLMSGVAFSCSMPLFPLIPPCTPLWLHGLGITALCL
                     MS"
     gene            5021..6673
                     /locus_tag="PK856_03425"
     CDS             5021..6673
                     /locus_tag="PK856_03425"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRL01457.1"
                     /translation="MNTLIQQLHHLAASKQVMAYLQADQRTYADFYQAVGQTAQQLAQ
                     QSESTWALWCEDSYDFTVHFFALLLAGKTVILPPNRQESTVKQLAEEGIAYFDGMTVH
                     TPDVSPPLTWPNIAWQQAQVVFYTSGSTGTPKKIHRTMGQLMCEIDVLHALFPWADKA
                     VVFATVSHQHLFGLCYKILLPLQQGCAFVNEQCLYPEDLEQVYASLNPTDAVLVSSPA
                     FLSRWQEPQAACKYLQIFSSGGPLPAQVPEQLQHDIVEIFGSSESGGIAWRPAPQAWQ
                     VFPNVRMRIDASEQLWIQTEHAYTADWISTGDAARALQDGHSFELLGRLDRMIKLEEK
                     RLSLDAIEKVLLQQTALTDVHVCVIPHKDTQQLAAVCALSADAWTQLREQGKHGFVQS
                     LKRRLQGQLESIAIPKRWRFIQALPRNSQSKLNHAAMLALFSLQKRPVSQVLQKNPDS
                     AEILLWFPPELQGFQGHFPGFAIYPGVAQLDMLQHHIRSLFLPEHVCVGVEQLKFQQP
                     IRPYDEAVLQLQYSDLKVLFKLMRDNTSLASGRLYFEAATGV"
     gene            6666..>6891
                     /locus_tag="PK856_03430"
     CDS             6666..>6891
                     /locus_tag="PK856_03430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRL01458.1"
                     /translation="MCKIRIGAVVPVYNHPRHLKRLVKHLHEQQLAVILVDDGSTDES
                     AMMIDVLQQEHAERVHVIRHPHNRGKGAAVR"
BASE COUNT         1830 a         1723 c         1564 g         1774 t
ORIGIN      
        1 atgctggaga cctactcgtc gtagacaaat ctttgcatgc aaagcaaggg gacattgtgg
       61 tggctgatat cggccatgaa ttcaccatca aacgcttgca cttacacggt ggcatgagtt
      121 tgcatccaga aaaccacctt gaaaactacc ccatactcta tccacaagca gaacaggaat
      181 ggcgattggt aggcgtgatc acctatgtca tcaaatcggt gcattcctca tgtatgcgct
      241 gatagatggc aaccattttt attgctcatg cgaacgggta tttcggcctg atttgaagtc
      301 acgtcctgtg gtggtgctca gcaataacga tggctgcgtg gtgtctcgaa gtccagaagc
      361 caaactattg ggcatcaaaa tgggggttcc ttattaccaa gtcgcacact tgcaaaaagc
      421 caatgaactg gtggtctttt ccagcaatta cccgctgtac tcgcatttgt ccaatcacat
      481 gatgcgcagc attgccagct tggttccaca cacagaggtg tattccattg atgaatgctt
      541 tgcagacgtt acgggcttga gtgatttagc cgaattggcc acaaacattc gtgaacgcgt
      601 gctgacttgg gtgggcatcc cttgttgtgt gggagttgca cccacgaaaa ccttggcaaa
      661 actgtgcaat catttggcca agaagcatgc ttgtttcaac agcgttgtgg tttggggcaa
      721 ctggaggagc gacatccaac aacgtgcctt gcgcagtgtc gacatcaatg aagtatgggg
      781 cataggacgc aaattcaacc aacgcttgcg tttgctgaac attcataccg catgggattt
      841 atgccaagct gacactgcct cattgcgcaa acaatttggc gtcaccttag agcgtaccca
      901 acgggaatta caaggcattg cctgtttgga tttggaatcc caagctccag ccaaacaaag
      961 catcttgtgc agccgcagct ttcaaagcca agtcacccgt ttggatgact taagtgccgc
     1021 actcacccac catgtgcaag aagccacctt caaactcagg catgaacaaa gccattgcca
     1081 ctacctgcgt gtcttcgctt acagcaaccg ctttcgtgaa gatttaccac agtattacgg
     1141 ccaccaaggc atccacttac ctgagccttg cgacgatacc ctatggctgc aacatctggc
     1201 tcaacaacaa ttgcgcgacc tgtttcaggc cggtatttct tacaaaaaat gcggggtgga
     1261 gttggcgatg ctgcaaaaga aacacatcac acaaatggat tggattgaga gcaccgtcac
     1321 cagaaagcag cggcctgaat tgatgcaagc atgggatgcc attaaacgca aatatggcga
     1381 caaacagctg catttggcca gcgaggattt gggctcggaa gcatggaaaa tgcgtcaaca
     1441 attcaaaagc cccaattaca ccaccaactt caaagacatc ttggtcatac ccaactgaaa
     1501 cacatcaaat atgcggcctg taacgcctat ttcacacgca aatccttttt aagcttcacc
     1561 ccttgtatgg ccatatgcag tattctcatt cagggtattg gtattgaaat cacccaaaaa
     1621 atacatttaa aattcagcga tacattcctc aaggctgatc tgtcgtacca tctagccgca
     1681 ttattactgc ttatgatgtc acctcaccaa taaaccctgt aatgcgtcag ccagaagcgc
     1741 cgttttacct tctttaaaaa gcaaaggaca agtcttgaat cgatttttaa tcatcctcat
     1801 gtctactgct attgcggcct gtaacgccat accaaataca acactcatta ccgtacaaaa
     1861 accgcttgga acattacagt gtcaagacaa tgacaacact gaacaccgat taattcaatt
     1921 atcaacacag ctaaaagagg cagaaatcaa gattgcatcg gctgcaatat cccatgatgg
     1981 tcgctcacgc ccaacagtgt gcggagcagc cgatggtaaa attggcttat ttaaaattcc
     2041 gttcaagcag cttcagcaag cacaacaact tgggtattct ttacgggaat cagattgaag
     2101 gttttataat tcatcaaaca gcgcctcatt aaggggcttt gttttggttg atagatactc
     2161 attaatcggc aacagataag aaataaggac aaccgcaatt caaaatattt acagaccgct
     2221 tgtacactcg cccacggtat ttttcaaaca ctccctgatt ctttctctcc cacccataag
     2281 tattgaacag tggttttata ggaatataaa aattcacttt gtgcattcaa acccttaacc
     2341 agtcgtgctt tcaatggcta taaaatcaac acagcataaa tattgcaacg caacattaga
     2401 gcactggctg cgataactat ttatgttaga attctatttc gtgtgattat tttaatttga
     2461 atgtttgcat tggttgcaat atgactgcca cactgcattt aaagtccttg ttccattggt
     2521 ctgcgcaaga tgatgtgccc ttaccagaat tggctgccat tgatgccaaa cagcgccgac
     2581 gcctgtcgtc attggccaag ctgtctttgg ctgcgaggca agccttagag caagaagtgg
     2641 acttgacact gaccgaccac attttatggt gttcaagcta cggtgatgaa cacaaaacct
     2701 tggccatcat gcaagacata gcccgcgacg aagcaccgtc gcccacggct tttgccacat
     2761 cggttcacaa tgccatcgct ggcttgtatt ccatgctgta ccaagacgat gtggccacca
     2821 ccagcatgaa tgtgggtcat accgagtttg gcaatgcgct ctggtatgcc catgccttgc
     2881 tacaaaccca tgcagccaat caagtgttgt tggtgtgtgc tgatgagcct ttgccagacg
     2941 tgtatgcaca acaagattta caacaggctt atgccatgac ttgtgtgctt tgccgacccg
     3001 atgaacacca tcccgcaaac ttacacatcg gcgctacaga acagcgggcg caaacccagc
     3061 tattgcaagc tcaagcggta cgttttgctg agttttggca aagcaatgcc accacgttac
     3121 acatggatgc ttggcaacta tgcaaacggt agatggtcga ctggcccatt actggcgcat
     3181 catcgccacg ggcatcagtt ttaccgtctt tggtgtgggt ggcttatgca tgggcttgct
     3241 tgccatgcct ttattattgc tgatgtgtcg caaccgtgag caacgccgat tacgggcgca
     3301 gtattggata caccgaggtt ttcgatggtt tattcgcttg atgcaagctt tgggcgtgat
     3361 gcgtttagaa gtcagcggtc tcgataggtt acaggccaat gttcctcaat tggtattggc
     3421 aaaccaccca agtttattgg atgtggtgtg tttaatcagc atcatgccgc aagccgattg
     3481 cattgtgaaa caggctttgt ggcgcaatcc atttatgcgt ggtgcggtac aagctgccga
     3541 ttacattcta aacgaccaat cggaacaatt gctgcacaac agcgtaaacc gcttacatgg
     3601 caacaacaaa atgattgtat ttcctgaggg cacacgcacc gccaaaaaca ccgtgctcaa
     3661 tccgtttcaa cggggagccg cacacattgc cattgccagc gaagcagaaa ttttacctgt
     3721 attgatacgc tgtacacctt ccaccctcag caagaacgaa gcgtggtacc atgtgccgcc
     3781 acagcgattt gtcatttctg tggattttca agacagcata gatgtggcaa catggcgaca
     3841 aatcgatgca gcgccaacca tacaagtcag gcgcttgaat gcttttttac acgattattt
     3901 tttaaggaaa ctcggccatg agtgatttac atttagaagt caaacaaatg atcatcgatg
     3961 tgttggcttt ggaagatgtc agcgctgccg acatagacga tgctgcgcca ttgtttggcg
     4021 atggattggg tttggactcc atcgatgcct tggaattggg tttggccttg aaaaaacgct
     4081 ataacatcaa gctcaaccaa gacagcgccg aaaccaaaca acactttagc tcagtcaatg
     4141 ccttggtcgc cttgattcaa gcacaagcct aattcaaacc gaacacagaa cacagcctcg
     4201 agtacacctt atgcaaacac gtgaagacat ttttgttgcc ttaaaacaat ggatggtgga
     4261 tttgtttgaa atcgatcccg ataaaatcac catggatgcc cacctatatg aagatttgga
     4321 catagacagc atcgatgcag tcgatttggt ggtgaaaatc cgtgaaatca cgggcaaaca
     4381 aattcaagcc gaagaattca agcacgtgcg tcgcatcagc gatgtggtgg atgccattgc
     4441 caaactgaac tgagcccatc cagatacatg caacactcct tacccatcaa aatcctgttg
     4501 gtggtagcat ttttgtgcta cccctttttg atgctgtttg gcctcagcca ttgggggatt
     4561 acccccattg ctttggtctt gatggtggct accctcttaa aactgctggt atccaaaaac
     4621 agtcagctca aaccgctgta tgttttgggc atcctctgtg gtttgctcag tctgctgcgc
     4681 caagatgaga tgtgggtaaa attttacccg gcctgtatga atgtgggctg gcttttggtc
     4741 tttgcttggt cgctacacac ggatcaatcc atgatagagc gcattgcccg cacccatgaa
     4801 cccgatttgc cccttctggt gtggcatgga cacgcaaagt cacttatgtc tggtgtggct
     4861 ttttcctgtt caatgccact gtttccgctt ataccgcctt gtacgcctct ctggctacat
     4921 ggacttggta taacggcttt gtgtcttatg tcttaatggg ctgtttgttc actggcgaat
     4981 ttgtattgcg ccattttcac aaagcccgcc ttgccaaaat atgaataccc tcatccaaca
     5041 attacaccac ttggccgcct ccaagcaagt gatggcctat ttacaggccg atcaacgcac
     5101 ttatgccgac ttttaccaag ctgtcggtca aaccgcacaa cagcttgccc agcaatctga
     5161 atcaacgtgg gcattgtggt gtgaagacag ctatgacttt accgtgcatt tctttgcctt
     5221 attgctggca ggcaaaaccg tgatactgcc acccaatcgc caggaatcca cggtcaaaca
     5281 attggctgaa gaaggcatcg cctattttga tggcatgaca gtgcatacac ctgatgtctc
     5341 tcccccctta acctggccca acatcgcttg gcaacaggct caagtggtgt tttacacctc
     5401 aggctcgaca ggcacgccga agaaaattca ccgcaccatg ggtcagttga tgtgtgaaat
     5461 cgatgtcttg cacgccttat tcccttgggc agacaaggcc gtggtctttg caaccgtgtc
     5521 acaccagcat ttgtttggcc tgtgttacaa aatcctgttg ccgcttcaac aaggctgtgc
     5581 ttttgtgaat gagcaatgtc tctaccctga agatttggaa caggtatacg cgtctttaaa
     5641 ccccactgac gcagtattgg tcagcagccc tgcctttttg agccgttggc aagagccaca
     5701 agcggcctgt aaatacctgc aaatcttttc ctcaggtggc cctttgcccg ctcaagtacc
     5761 tgaacaatta caacacgata tcgtggaaat tttcggctca tccgaatcag gtggcattgc
     5821 atggcgacct gcaccgcaag catggcaagt gtttcccaat gtgcgcatgc gcattgatgc
     5881 gtcggaacaa ctgtggatac aaaccgagca cgcttacact gccgattgga tcagcacggg
     5941 cgatgctgct cgtgcactac aagatggcca cagttttgaa ctgttgggca ggttggaccg
     6001 catgataaag ttagaagaaa aacgcctgtc tttggatgcc atcgagaaag tattgttgca
     6061 acagacggca ctgaccgatg ttcatgtgtg tgtgatacca cacaaagaca cacaacaatt
     6121 ggcagctgtc tgcgccttaa gtgcagatgc gtggacacaa ctgcgcgaac aaggcaaaca
     6181 tggtttcgtg caaagcctga aacgccgctt acaagggcaa ttggaaagca ttgccattcc
     6241 taaacgctgg cgttttatcc aagcattgcc ccgcaacagc caatccaaac tcaaccacgc
     6301 ggcgatgttg gccttatttt ccttacaaaa gcggcctgta agccaagttt tgcaaaaaaa
     6361 tcctgacagt gccgagatac tgttatggtt ccctcccgaa ttgcagggtt tccaagggca
     6421 ttttcctggt tttgccatct acccaggagt cgctcaacta gacatgttgc aacaccacat
     6481 ccgcagtctg tttttgcctg agcatgtgtg tgtgggtgtg gaacagctga aatttcagca
     6541 acccatacgc ccttatgatg aagccgtgtt gcaattacag tacagcgatt tgaaagtctt
     6601 gttcaaactg atgcgagaca acacttcttt ggcttcaggc cgcttgtatt ttgaggctgc
     6661 gacaggtgtg taaaatacgc ataggagccg tggtaccagt ttacaaccac cctcgccatt
     6721 taaagcgctt ggtcaaacac ctgcatgagc agcaactggc ggtgattttg gtggacgatg
     6781 gcagcaccga tgaatccgcc atgatgatag atgtgctcca acaagagcat gctgaacgtg
     6841 tgcatgtcat caggcatcca cacaatcgcg gcaaaggtgc ggcagtgcgc a
//
LOCUS       DAOMVK010000062         6860 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_2978_length_6860_cov_2.415283, whole genome shotgun sequence.
ACCESSION   DAOMVK010000062 DAOMVK010000000
VERSION     DAOMVK010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 6860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6860
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2978_length_6860_cov_2.415283"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="PK856_03435"
     CDS             <1..642
                     /locus_tag="PK856_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HRL01459.1"
                     /translation="DKKEVTRYCAIAALIAGVALAAVYLALGWAGNHYPLDAATLSQL
                     NADGTHLGVYLLVNIASATYGAIGPWLVAIIVLLACLTTAIGLIVAVSDYFNQQFSML
                     SYKSYVWIFTLISLLLANQGLNQVISLSIPVLLILYPIAMTVIVLLLLDPIFHPARWV
                     YRSTVGTAVVISLLSQFVPAVVQSLPLADASLAWLLPVLAVFIAGSVLKQIKP"
     gene            766..1308
                     /locus_tag="PK856_03440"
     CDS             766..1308
                     /locus_tag="PK856_03440"
                     /inference="COORDINATES: protein motif:HMM:NF027429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4822 domain-containing protein"
                     /protein_id="HRL01460.1"
                     /translation="MKITLRIAILGVSSVLALAACSGAKTQAVNTGTTAADMNVSELS
                     LLKTGTWVTRSIHDANGLIYSGKDEFVARLAGVTRYHNNGTFRSVSMDNTLVFEGNWN
                     ISDDGKILNLTGVGKDGRPGFTIAAPVLKLNNTEFSYRVYPNPNNRNQYFDVKLGPAS
                     IEESKILNRASSTNPNSMSN"
     gene            complement(1364..2266)
                     /gene="prmB"
                     /locus_tag="PK856_03445"
     CDS             complement(1364..2266)
                     /gene="prmB"
                     /locus_tag="PK856_03445"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRL01461.1"
                     /translation="MYTSAAQHLSTIRDVFRYTISQFNAAELFYGHGNDNAFDEAAYL
                     IVHCLQLPSDALDTFYDAKLLPDEIDAILSVVYERIDTRKPLAYLTNEAFQGEFDFYV
                     DERVLVPRSFIYELLGEPLSPWLEYPELVHNALDLCTGSGCLAIQMAHHYPAAEVDAV
                     DLSLDALEVAAINVERYGLEERVQLIHTDLFEGIEQTYDLIISNPPYVDAESVDALPE
                     EYLHEPEMALGSGDDGLDATREILLQAAKYLNPRGVLLVEIGHNREVLEAAYPELPFM
                     WMETSGGDGFVFLLTREQLLGQEA"
     gene            2720..4750
                     /gene="recG"
                     /locus_tag="PK856_03450"
     CDS             2720..4750
                     /gene="recG"
                     /locus_tag="PK856_03450"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRL01462.1"
                     /translation="MNLVDLGVTDKVQAKLAKLHIHNVWDAVLHLPLRYEDETHITMI
                     ADAKLGESVQVEGEVLLQEVQFKPRKQLVVKLQDDAGSVLMLRYIHFYPSQQQQLSTG
                     KRVRAVGEIKQGYYGDEMIHPKIRAADNHALSETLTPIYPTVSGLTQPMLRKIVHAAL
                     SKANLDDTLPKTLLQQLHLPGFADSVRLLHDPTPEYSTQQLHDDKLPAWQRLKFDELL
                     AQQLSMKLAYAQRCNGKAPILNGDGVWRARLLAALPFQLTAAQQKVLTEIEHDMLQSY
                     PMHRLLQGDVGSGKTIVSALAALNAIEAGAQVAVMAPTEILAEQHLQKFSQWLAPMGI
                     EVAWITGSTRKKAKDAAKAGLADGSIRIAVGTHALFQDDVVFDNLGLVIVDEQHRFGV
                     GQRLALKNKGAEVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPNRTPIKTKLINAG
                     RRDDIEAFVRHTVQKGQQVYWVCPLIEESETLQLQTAVDTHIELQAAFPEYSVGLVHG
                     RLKAVDKAAEMQKFIRNEHQVLVATTVIEVGVDVPNASLMVIEHAERMGLAQLHQLRG
                     RVGRGQAASVCVLMFTEPLSELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLGAKQS
                     GVPMLRFANLEEDVDLLVQARDVAQVLIANKSEVVEQHLQRWLASREAYLGV"
     gene            complement(4780..4992)
                     /locus_tag="PK856_03455"
     CDS             complement(4780..4992)
                     /locus_tag="PK856_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01463.1"
                     /translation="MSALQLIKGKQQEISLHEKIAQQLARLNEHNKKHCLEMIMALEA
                     EERLLFLNAFMMAVAALPATAAVAAA"
     gene            5257..6189
                     /gene="rarD"
                     /locus_tag="PK856_03460"
     CDS             5257..6189
                     /gene="rarD"
                     /locus_tag="PK856_03460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HRL01464.1"
                     /translation="MVVSRKGVAAVLLSNVLFGVLYLYSHFMQPMVGSDVFAWRMVAM
                     LVALSVMMSMSGGWAALGKFVRRINQQPKQWFLFVGSTFVVGGNLWVFMWAPVNGYGI
                     DVAVGYFLLPLTTLLMGMVVFGERLNKWQKWAVAFAGVGVMHEMWVAESVSWITLFCC
                     LSYPFYFGIRRHLQTPALVGLWMDLMLIAPFAIAYLVFFSDSMTLVASQWHFTPLIIS
                     LGVFSALAMCWVLYGARTLPFTLFSMLTYGEPILLFILAVVFMNAPLTGSAMITYGFV
                     WVGLMLMVWDGCLQMRQQRGDKAVSEASTSASSS"
     gene            complement(6356..6553)
                     /locus_tag="PK856_03465"
     CDS             complement(6356..6553)
                     /locus_tag="PK856_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01465.1"
                     /translation="MNNNRWITLRSVGLVWFAYAVLFWLLHTHTPWPGGSMGSMAVLS
                     VTLWILAAKLINRKQHPHTHT"
     gene            complement(6587..6754)
                     /locus_tag="PK856_03470"
     CDS             complement(6587..6754)
                     /locus_tag="PK856_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01466.1"
                     /translation="MYAVAIIIGLAVCLTTYYAIHRRQIVPVLASAAATMAALLIGKA
                     LPNAWQMDTEL"
BASE COUNT         1748 a         1450 c         1802 g         1860 t
ORIGIN      
        1 gataaaaaag aggttacccg atattgtgcg attgcggctt tgattgcagg ggtggctttg
       61 gcagctgtct atctggctct gggctgggcg ggcaatcatt acccacttga tgcggcgact
      121 ttgagccaac tcaatgccga tggtactcac ttaggagtgt atttgttggt caacattgct
      181 tcagcaacct acggggccat tggaccatgg ttggtggcca ttattgtgtt gttggcatgc
      241 ttgaccactg cgataggttt gattgtggcg gtatctgatt atttcaatca gcaattcagc
      301 atgttgtctt acaaaagcta tgtgtggatt tttactctaa tcagcttgct gttggccaat
      361 caaggcctga accaagtcat cagtttgtcg atacctgtgt tgttgatttt gtatccgatt
      421 gcgatgacgg tgattgtctt gttgttgctt gacccgattt ttcatcctgc ccgttgggtg
      481 tatcgcagta cggttggtac ggcggtggtc atcagtttgt taagtcagtt tgtgcctgct
      541 gtggttcagt ctttgccttt ggcggatgct tccttagctt ggttattgcc tgtgttggct
      601 gtgtttattg caggcagtgt tttgaagcaa atcaaaccat aatccagtga gacagaactt
      661 tataagcgtc ctgtaatcgt acaggacgct tattgtttat tggtggcaca gatgcaatga
      721 gcactatgat aaaaggacaa tgaacaacac atagaggaga tgggcatgaa gatcacctta
      781 cggattgcga tattgggtgt gagcagtgta ttggctttgg ctgcttgtag tggtgcgaaa
      841 actcaggctg tgaacacggg tacgacggct gccgatatga atgtttccga actgagcttg
      901 ctcaaaacag gcacatgggt tacacgcagc attcatgatg caaatggctt gatttacagt
      961 ggtaaagatg aatttgtggc acgtttggca ggtgttaccc gctaccacaa caacggcaca
     1021 ttccgcagcg tgagtatgga caataccttg gtatttgagg gcaattggaa catcagtgat
     1081 gatggaaaaa tcttgaattt aaccggtgtg ggcaaagatg gccgcccagg ctttacgata
     1141 gcggctcccg tgttgaaact gaacaatacc gaattttcat accgagtgta cccaaatccg
     1201 aacaatcgca atcagtattt tgatgtgaaa ttgggacctg ccagcataga ggaatccaaa
     1261 atccttaacc gcgcatcttc taccaatcca aacagtatga gcaattaaac ccaaattaag
     1321 cggcctgtaa atgtttacag accgcttaat agcaatgagg atgttaggct tcttggccca
     1381 acaattgttc acgcgtcaat aagaatacaa agccgtcccc accactggtt tccatccaca
     1441 taaacggcaa ttcaggataa gcagcttcca atacctcgcg attgtgacca atttcaacca
     1501 gcaatacccc gcgtgggtta aggtatttag cggcctgtaa caaaatttct cgagtcgcat
     1561 ccaagccatc atcgccagaa cccaaagcca tttctggctc gtgtaggtat tcttctggca
     1621 aggcatcgac actttcggca tctacatacg gcggattgga aataatcaag tcgtaggttt
     1681 gttctatgcc ttcaaacaaa tcggtgtgta tcaattgcac acgctcttct aagccatagc
     1741 gctccacatt gattgcggcc acttccaagg catccaagct caaatccacg gcatccactt
     1801 ctgccgcagg gtagtggtgc gccatttgta tggccaaaca gccgctgcca gtgcacaagt
     1861 ccaaagcgtt gtgtaccaat tcagggtatt ccaaccaagg tgataagggt tcacccaata
     1921 attcataaat gaatgaacga ggcaccaaaa cgcgctcatc cacatagaaa tcaaattcac
     1981 cctgaaaagc ttcgttggtt aaataagcca gaggtttgcg ggtatcaatg cgttcgtaga
     2041 ccacagataa gatggcatca atctcatcgg gcagcaattt ggcatcgtaa aaagtgtcga
     2101 gagcatctga gggcaattgc aaacaatgca caatcaaata ggcggcttca tcaaaagcat
     2161 tgtcattgcc gtggccatag aacaattcgg ctgcattaaa ctggctgatg gtatagcgaa
     2221 aaacatcgcg tatggtggat aaatgctggg cagcagaagt gtacatggct tgcctattca
     2281 aaacgataaa atggccgtca ttataacgta agcggcctga aagtgatatt tgtcaccata
     2341 cgggcaacat ggctacaaat ttaggcggta agcgatgaat taccctggtt ttgagcattt
     2401 atttgaggta tctcaatcta tttaacatga gaatgacata tactaattgc ataaaaattg
     2461 aatatcaatt gcatcacaaa atgcacaatc aaactgtaaa acagtcattg atggataagg
     2521 gaacaaaagc gtcttattgc taaggcgctt tttctttgcc tgatatttac aggccgcatg
     2581 tgactttgct gaaacaagga ttttcctgag ttggcatctg ggcgggcgtc atcatataat
     2641 tgtgattgat tatttattgg tagcaaacag tattgattta tgcaattttt tcatcacaca
     2701 caatcaggtc aaccatcata tgaatttagt tgatttgggt gttaccgaca aagtccaagc
     2761 caagctggcc aaactgcaca tccacaatgt atgggatgcg gtgttgcatc tgccgctgcg
     2821 ttacgaagac gaaacccaca tcacgatgat agccgatgcc aaactgggag aatcggtgca
     2881 ggtggaaggt gaggtcttgc tgcaagaagt gcaattcaaa ccacgcaaac aattggtggt
     2941 gaagctgcaa gacgatgcgg gcagtgtgtt gatgctgcgt tacattcatt tttacccgag
     3001 tcaacaacag cagttaagca caggcaagcg cgtgcgtgca gttggtgaaa tcaagcaagg
     3061 ctattacggc gatgaaatga tacacccgaa gattcgtgct gccgataacc acgctttatc
     3121 agaaaccctt accccaattt atccgactgt cagtggtttg acccagccga tgttgcgcaa
     3181 aatcgtgcac gccgcgttga gcaaagcgaa tctagatgac accttgccca agacattgtt
     3241 gcagcaactg catttgcctg gttttgcaga cagtgtgcgc ttattgcatg accccacgcc
     3301 agaatattcg acccagcaat tacacgatga caaattgcct gcgtggcagc gcttgaaatt
     3361 tgatgaattg ttggcgcaac aattgtcgat gaaactggct tatgcacagc gttgtaatgg
     3421 caaagcgccc atattgaatg gtgatggggt atggcgggct cgattgttag cagccttgcc
     3481 gtttcaattg actgccgcgc agcaaaaagt gttgacagag attgaacacg atatgttgca
     3541 atcttatccg atgcaccgct tgctgcaagg cgatgtgggc agtggcaaga ccatagtatc
     3601 ggcattggct gctctgaatg cgattgaggc tggggcgcaa gtagcggtga tggcacctac
     3661 cgaaattttg gccgagcagc atttgcaaaa attcagccag tggcttgcgc ccatgggcat
     3721 agaagtggca tggataacgg gcagtacgcg caagaaagcc aaagatgcgg ccaaggcagg
     3781 tttggcagat ggcagcatac gcattgcggt gggcacgcat gctttgtttc aagacgatgt
     3841 ggtgtttgac aacttgggtt tggtcattgt ggatgagcag caccgatttg gtgtggggca
     3901 acgcttggct ttgaaaaaca aaggtgcgga agtgcaccaa ttgatgatgt cggccacgcc
     3961 gataccgcgc actttggcca tgagtttttt tgctgacttg gacgtgtcgg taatcgatga
     4021 attgccaccg aatcgcaccc cgattaaaac caagctcatc aacgcaggca gacgcgatga
     4081 catagaagca tttgtgcgcc atacggtgca aaaaggccaa caagtgtatt gggtgtgccc
     4141 gttgattgag gaaagtgaaa ccttgcaatt gcaaaccgcg gtagacaccc atattgagtt
     4201 acaggccgca tttcctgagt attcggtagg cttggtgcat gggcgattga aagcagtgga
     4261 caaagcggct gaaatgcaaa aattcatccg caatgagcat caggttttgg tggccaccac
     4321 agtgatagag gtgggggtgg atgtgcccaa tgccagcttg atggtgattg aacacgctga
     4381 gcgcatgggc ttggcgcaat tgcatcaatt gcgtgggcga gtggggcgag gacaagcagc
     4441 aagtgtgtgt gtgttgatgt ttaccgagcc tttgagcgaa ttggccaaag cccgtttaaa
     4501 agtgatttat gagcacaccg atggttttga aattgccaga caggatttga atatccgtgg
     4561 cccaggtgaa tttttggggg ctaagcaaag tggcgtaccg atgctgcgtt ttgccaattt
     4621 ggaagaagat gtggacttgt tagtgcaggc gcgtgatgtg gcgcaagtgt tgatcgccaa
     4681 taagagcgag gtggtggagc agcatttaca acgctggttg gccagccgtg aagcttattt
     4741 gggggtgtaa atgcatcacg agttggcgaa gaatcaaacc taagctgctg ccacagccgc
     4801 agtggcaggt aaggcagcga ctgccatcat aaacgcattc aaaaacaaca agcgctcttc
     4861 agcctctaaa gccatgatca tttccaagca atgctttttg ttgtgttcgt ttaagcgagc
     4921 caattgttga gcaattttct cgtgcaagct gatttcttgt tgcttgcctt ttatcaattg
     4981 tagtgcgctc atggccattt ccttcaactt gcgggtgttt tctagagcaa tgcaagcgct
     5041 atgccaataa aataaagtaa gctgccaaaa ggcaaagcta agaaaagcgc tgtttcaact
     5101 catgttcatc aaggctctaa acggctcaga aatgatgact gaggtaaaaa attaacatta
     5161 tgcagggtaa agtgcagaaa caccttgtat tcaaagactt gttgatttga ataagatata
     5221 attaaacttt caaaaatttt gcttgggttg ggtgtcatgg tggtatcgcg taagggtgtc
     5281 gctgcggttt tattgtcgaa cgtattgttt ggcgtgctgt atttgtacag ccattttatg
     5341 cagcctatgg tgggtagcga tgtgttcgca tggcgcatgg tggcaatgct agtggcattg
     5401 agtgtgatga tgagcatgag cggtggttgg gctgcgctgg gtaaatttgt gcgccgtatc
     5461 aatcagcagc ccaagcaatg gttcttgttt gttggctcca cttttgtggt gggcggcaat
     5521 ttgtgggtgt tcatgtgggc gcctgtgaat ggctatggca ttgatgtggc ggtgggctat
     5581 tttctgttgc ctttaaccac cttgctgatg ggcatggtgg tgtttggtga acgcttgaac
     5641 aagtggcaaa agtgggcggt ggcgtttgct ggcgtgggcg tgatgcatga aatgtgggta
     5701 gccgaatctg tatcgtggat taccttgttc tgctgtttgt cttatccgtt ttactttggc
     5761 atacgccgtc acttgcaaac gcctgcactg gtgggcttgt ggatggattt gatgttgatt
     5821 gcgccttttg caatcgctta tttggtattc ttctctgaca gcatgacttt ggtggcgtcg
     5881 caatggcatt tcacgccgct catcatcagc ttgggtgtgt tcagtgcttt ggccatgtgt
     5941 tgggttttgt atggtgcgcg cactttgccg tttaccttgt tcagtatgtt gacgtatggt
     6001 gagccgattt tgctgtttat tttggcggtg gtgttcatga acgcgccatt aacgggcagt
     6061 gcgatgatta cctatggctt tgtctgggtg ggcttgatgc tgatggtgtg ggatggttgc
     6121 ttgcaaatgc gccaacagcg cggcgacaaa gccgtgagcg aggccagtac ttcggcttct
     6181 tccagctaag ttattgtgat tcaagcttaa agcctgttgt catatgcagc aggcttttta
     6241 attattaagc ggcctgtaag tctatttcat tgtgcttaca ggccgcttaa tgttctgatt
     6301 tcagattaag acagatgctt catggtcgtg tatacaagcg gcctgtaaac atggtttatg
     6361 tgtgtgtatg agggtgttgc ttacggttaa tcagtttcgc tgccaatatc catagtgtta
     6421 ctgacaacac cgccatgctg cccatgctac cacctggcca aggggtgtgg gtgtgcaaga
     6481 gccaaaacag cactgcatac gcgaaccaaa ccaagccaac actgcgcagg gtaatccaac
     6541 ggttgttgtt catgccacaa aaactgctgc caaaccaaaa caaatgctac agctcggtat
     6601 ccatttgcca agcgttgggc agagcttttc cgatgagtaa ggccgccatg gtggctgctg
     6661 ccgaagccaa aacgggcaca atttgtcgtc tgtgtatggc gtaatatgtg gtcaaacaca
     6721 ctgccaaacc gatgattatt gccactgcat acatgtgtgt acccccagca aaatcacgca
     6781 ggaaatgaag gcttgcatgc ccagacgacc gcctaatccg gttaacaagc ggttgctggc
     6841 caaatacacc atgctcgcca
//
LOCUS       DAOMVK010000063         6853 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_2983_length_6853_cov_3.300382, whole genome shotgun sequence.
ACCESSION   DAOMVK010000063 DAOMVK010000000
VERSION     DAOMVK010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 6853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6853
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2983_length_6853_cov_3.300382"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PK856_03475"
     CDS             complement(<1..453)
                     /locus_tag="PK856_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HRL01467.1"
                     /translation="MSSSKFNLSHITAGFTTVLVGYTSSVVIVIQAATAAGATAEQIQ
                     SWLWALGVGIGLICMLFSWWYKKPMVFAWSTPGAAMLVTVASQYDLPIVIGAFVMTGA
                     LIVLTGLITPLSHALARIPAYLGTAMLGAILLPFCLKAFQPLASQPISF"
     gene            557..1096
                     /locus_tag="PK856_03480"
     CDS             557..1096
                     /locus_tag="PK856_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HRL01468.1"
                     /translation="MNQDSFKTHIARHIKELRQQKGLSLDATAKLTQVSKAMLGQIER
                     EESSPTIATLWKIASGLETSFSAFFAEEAKLHEHEWAFPDDALMKVRTLFAFQADTGM
                     EMFEICLLNGHKQDSSPHGMGVIEHVLVLTGKMRVFYDKQWHELQAGESLRFFADQAH
                     AYEALGEEVVFQNIICYPK"
     gene            1165..1560
                     /locus_tag="PK856_03485"
     CDS             1165..1560
                     /locus_tag="PK856_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01469.1"
                     /translation="MKQFTTILGFALLGWCAQAMAEQCLLPKEHLRSVNCQIEAGAKS
                     HDWGSLVIRYENERVYFFQTNATTFLMNQREVQRLAQPTTANDNHASWGEAEGYDLNA
                     LKRPSKNQAEAVWACYAQTTGNTAICLSY"
     gene            1777..3378
                     /locus_tag="PK856_03490"
     CDS             1777..3378
                     /locus_tag="PK856_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HRL01470.1"
                     /translation="MLNMIQEQAKRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKSGKFATSDWMDIEKQRGISVASSVMQFDYRDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRMRNTPIVTFMNKYDREVRDSLELLDEVESV
                     LQIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGAEKLSHEFEVIQGINNPRLDELF
                     PLEMDNLRAEIELVQAASNEFNLEEFLAGELTPVFFGSAINNFGVREILDALVNWAPA
                     PHARAAVERVVEPGEEKFSGFVFKIQANMDPKHRDRIAFLRVCSGEFSRGMKIKHLRI
                     NREIAASSVVTFMSHERELVEEAFAGDIIGIPNHGNIQIGDSFSEGEMLTYTGIPFFA
                     PELFRGVRIKNPLKMKQLQKGLQQLGEEGAVQVFKPHNGGDLILGAVGVLQFEVVASR
                     LAAEYSVDAVFDSVSIWSARWVSCDDKKKLAEFERANAMNLSTDAGGNLTYLAPNRVN
                     LNLTQERWPDIVFHETRELNNALND"
     gene            complement(3537..4394)
                     /locus_tag="PK856_03495"
     CDS             complement(3537..4394)
                     /locus_tag="PK856_03495"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HRL01471.1"
                     /translation="MSPQRFVLNFAAPSQRGQVGRLTQILEQHGAYIEQFSVFDDNRC
                     QFFYLRSVFYIENPAFDLNALQNEYHEFLAAQGGAGHIWDANEAVKVLIMVSKTDHCL
                     NTLIAASKLGALNMDIVGIASNHLDLKPIADHHNIPYYHLPVNAETRARQEQQIIDLK
                     TQLDAEFVVLARYMQVLSNATCQILQKQAINIHHSFLPGFRGAKPYEQAYERGVKLIG
                     ATAHFVTPDLDEGPIIEQAVERVDHAYLPEDLLRTGRHSESVVLERALKSVLERRVFT
                     NGERTVILR"
     gene            4627..5901
                     /locus_tag="PK856_03500"
     CDS             4627..5901
                     /locus_tag="PK856_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HRL01472.1"
                     /translation="MSNGIFDKNNHYSWKSLLKNGLSNNQNWPQARRMATLQSQYDVI
                     IVGGGGHGLATAYYLAKNHNVGKIAVLEKGYLGGGNTARNTTLVRSNYLWEDAANLYE
                     HSMKLWEGLSDDLNFNTFFSQRGVFNLGHNLQDMRDIERRVNANNLLGIDAHVLTAQE
                     VQDKIPLLDCSRRSRYPVLGASWQPRGGIAHHDAVARGFARAASHLGVDLIEGCEVIG
                     MDISEGRIQGLQTTQGFVRANQVGVVAAGHTSVLAGMAGLRFPIESHPLQAFVSESMQ
                     KVFDTVVMSNAVHGYMSQSDKGEFVIGAGIDGYLGYGQRGSPHVIEHTAAAIVELFPT
                     LSRVRMNRQWGGIVDTTPDACPIISKTPVKGLYFNCGWGTGGFKATPGSGHVFAHTIA
                     NDNPHPLNRGFTLDRFHTGRLIDEHGAAGVAH"
     gene            5912..6220
                     /locus_tag="PK856_03505"
     CDS             5912..6220
                     /locus_tag="PK856_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HRL01473.1"
                     /translation="MLYIHCPFCAEKRDEQEFHYAGEAYIARPTDPENCSDEEWGDYV
                     FMRTNHKGWIWEQWEHVSGCRKVFVAKRNNVSNDIEATYNLVEGKQHFLEHFAVEESA
                     "
     gene            6217..>6853
                     /locus_tag="PK856_03510"
     CDS             6217..>6853
                     /locus_tag="PK856_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRL01474.1"
                     /translation="MSAKRILKPSVQVDGKQTLTFEFDGKNYSGLQGDTLASALLANG
                     VKALARSYKYGRLRGIMSAGAEEPNALVQMETGAHCSPNIKATQAELYAGLTANRTSG
                     WPSLQFDVKAVIGTVGKNMMAPGFYSKTFKWPQKMWPKYENVIRQFAGFGSTPEQADA
                     EWYDHLHHHVDVLVVGGGLAGLQAALISANNGLKVLLVDEQAQVGGWLLSDA"
BASE COUNT         1819 a         1586 c         1754 g         1694 t
ORIGIN      
        1 gaaagagatg ggttggctgg ccaacggttg gaacgccttc agacaaaaag gcaataatat
       61 cgcccccaac atggccgtac ccaaataagc agggatgcgt gctaaggcat ggcttaatgg
      121 agtgatcagg ccagttaaca cgattaaagc ccctgtcatc acgaaagcgc ctatgacaat
      181 gggcaaatcg tattggctcg ccacggtcac caacatggcg gctccaggcg ttgaccaagc
      241 aaacaccatg ggctttttat accaccacga aaacaacatg cagataaggc ctatgcccac
      301 acccaaggcc caaagccagc tttgaatttg ttcagccgtg gctcccgccg cagtagcggc
      361 ctgtatgacg atgacaacgg aactggtata gcccaccaag accgtggtaa atccagctgt
      421 gatgtggctg agattgaatt tagaagaaga catgtgcgta ctcgaatttg atgctaagat
      481 gatttgtgcg ttttaacgta cgcagaactg taacactgtg cgctttagcg cacaactgaa
      541 atgacaagga caaggtttga accaagacag ttttaaaacc cacatcgcca gacacattaa
      601 agagttgcgc caacaaaagg gattgagttt ggatgcaacc gcaaagttga cgcaggtttc
      661 caaagccatg ttgggccaaa tagagcgcga agaatccagc ccgaccatcg ccacattgtg
      721 gaaaattgcc agtggcttag aaacctcttt ctctgccttc ttcgcagaag aagccaagct
      781 gcatgaacat gaatgggctt ttccagacga tgcgctgatg aaggtgcgca cattgtttgc
      841 gtttcaggcc gatacaggca tggaaatgtt tgaaatttgc ttgctcaacg gccacaaaca
      901 agactcttcg ccgcatggca tgggcgtgat tgaacatgta ttggtattaa caggcaaaat
      961 gcgtgtgttt tacgataagc aatggcatga attacaagca ggtgagagtt tgcgtttttt
     1021 cgccgaccaa gcccatgcct atgaggcact gggtgaagaa gtggtgtttc aaaacatcat
     1081 ttgttacccg aaataattgg ggtttatggc ttaatctgaa tatctgacta ctggtatgta
     1141 agcgcatttt acaaggcaga aatcgtgaaa caattcacca caattttggg tttcgcgcta
     1201 ttaggctggt gtgcccaagc catggctgaa caatgcttgc ttcctaaaga acatctgcgc
     1261 agtgtcaatt gtcaaattga agcaggcgct aagtctcatg attggggcag tttggtcatc
     1321 cgctatgaaa acgagcgtgt gtatttcttc caaaccaatg ccaccacatt tctcatgaac
     1381 caacgagaag tacaacgctt ggctcaacca accacagcca atgacaatca tgcttcatgg
     1441 ggtgaggcag agggctatga tttaaacgca ctcaaacgtc ccagcaaaaa tcaagccgaa
     1501 gccgtgtggg catgttatgc acaaaccaca ggcaataccg ccatttgctt gtcttattaa
     1561 agcaatcctc aacgatgtat aggaaaaagg ccaaataaat ggttaatgat gtgagctgat
     1621 agaaagattt tcttaaatat cggcctgaat ttgctataat tgccgccatt atttttagac
     1681 ttggtcatta ccaagtcttt tgcattttta tacaacgacc aatcaaatct aatgtgtaca
     1741 tgattgtgta tgtgcataat ttcaaaaagc gtatttatgt taaacatgat tcaagagcag
     1801 gcaaagcgcc gcagaacgtt tgctatcatt tcacatcccg atgcgggtaa aaccacttta
     1861 accgaaaaac tgttgctgtt ttcgggtgcg attcaatcgg cgggtacggt taagggcaag
     1921 aaatcaggca aatttgccac ttcagactgg atggacatcg agaaacaacg tggcatttcg
     1981 gtggcttctt cggtgatgca gtttgattac cgcgaccaca ccgtgaattt gctcgacacc
     2041 ccaggtcacc aagacttctc ggaagacact taccgcgtgt taacggcagt agacagcgct
     2101 ttgatggtga ttgatgcggc caagggtgtg gaagcgcaaa ccatcaaatt gttgaacgtg
     2161 tgtcgcatgc gtaatacgcc gattgtcacc ttcatgaaca aatacgacag ggaagtgcgc
     2221 gactctttgg aattgctcga tgaagttgaa agtgtgttgc aaattcgctg tgcgcccgtg
     2281 acttggccta tcggcatggg caagaatttc aaaggtgtgt accacatctt gaacgatgag
     2341 atttacctgt ttgaagcggg tgcggaaaaa ttgtcacacg aatttgaagt gatacaaggc
     2401 attaacaacc cgcgcttgga tgaattgttc ccattggaaa tggacaatct gcgcgctgaa
     2461 atcgaattgg tacaggccgc ttccaatgag tttaatttgg aagaattctt ggcaggcgaa
     2521 ttgacacctg tgttttttgg ctcggcgatt aacaattttg gtgtgcgcga aatcttggat
     2581 gccttggtga actgggcgcc tgcgccgcat gcccgtgctg ctgttgagcg cgtggttgaa
     2641 cctggcgaag aaaaattctc gggttttgtg ttcaaaattc aagcgaatat ggacccgaaa
     2701 caccgcgacc gcatcgcgtt cttgcgcgtg tgttcgggtg agttttcgcg tggcatgaaa
     2761 atcaagcatt tgcgcatcaa ccgcgaaatc gcggcttcca gcgtggtgac cttcatgtcg
     2821 catgagcgcg aattggtgga agaagcattt gcgggcgaca tcatcggtat tcctaaccac
     2881 ggcaacatac aaattggcga cagcttctcc gaaggcgaaa tgctcactta cacaggcatt
     2941 ccttttttcg cacctgaatt gttccgcggc gtgcgcatta aaaacccatt gaaaatgaag
     3001 caattgcaaa aaggcttaca acaattgggt gaagaaggtg cggtacaggt attcaaacca
     3061 cacaacggtg gcgatttgat tttgggcgcg gtcggcgtgt tgcagtttga agtggtggct
     3121 tcgcgcttgg cagcggaata cagcgtggat gcggtattcg attcggtgag catttggtcg
     3181 gcacgctggg tgtcttgcga tgataagaag aaattggccg aatttgaacg cgccaatgcg
     3241 atgaatttgt ctaccgatgc cggtggcaat ttgacttatt tggcgcctaa ccgcgtcaat
     3301 ctgaacctaa cgcaagagcg ttggccagac attgtgttcc acgaaacccg tgaattgaac
     3361 aatgctttga acgattaaac ttgatggtat gttttgcaag taatgaataa gcggcctgta
     3421 aacagttaca ggccgcttat tgtatggctt aaacagttta tacattctaa accatggcat
     3481 ggcaaagcta aaaaagcgcg gccaattctt aaacctacca gtcacaaaaa atcttgttaa
     3541 cgcaagataa cggtacgttc gccattggtg aatacgcgac gttccaacac gctttttaaa
     3601 gcccgctcca acaccacgct ctcactgtgg cggcctgtac gcaacaagtc ttcaggtaaa
     3661 taagcatggt ctacgcgctc aaccgcttgc tcgataatcg ggccttcatc caaatcaggg
     3721 gtcacaaaat gcgcggtcgc acctatcagt ttcaccccgc gctcatacgc ttgctcatac
     3781 ggcttggcac cgcggaagcc tggcaagaat gaatggtgaa tgttgatggc ttgtttttgc
     3841 aaaatttggc aagtggcgtt gctcaatacc tgcatgtagc gcgccaacac cacaaattcg
     3901 gcatcgagtt gggtttttaa atcaatgatt tgttgctctt gacgcgcacg cgtttctgcg
     3961 ttcacaggca agtggtaata cggaatgttg tggtggtcgg caatgggttt caaatccaaa
     4021 tggttggagg cgatgccaac aatgtccatg ttcaaagcgc caagtttgct cgcggcgatt
     4081 aaagtgttca agcaatgatc ggtttttgaa accatgatca agacttttac cgcctcattg
     4141 gcatcccaaa tgtggcctgc gccgccttgt gcagccaaaa attcatggta ttcattttgt
     4201 aaggcattca aatcaaatgc ggggttttcg atgtaaaaca cgctgcgcaa atagaaaaat
     4261 tggcaacggt tgtcatcaaa cacggaaaat tgctctatgt aagcaccgtg ttgttccaaa
     4321 atctgggtta agcgaccgac ttggccgcgt tgacttgggg ctgcgaaatt caaaacaaat
     4381 cgttgtgggc tcatcatcgt cttccattgt aggttttatt caatatgcgc ttgtgctcgc
     4441 gcggtaaaac atcatggcaa attatgcctg tttgcaatca tccaattgcg acatctaagg
     4501 cgttttttgc gacaaatgat gtcgtgtcgg ttttagaaca gtttttgtct tgacatgacg
     4561 gttttaaagc cgccaatcgc caataatgca aaccataaca gcgtgctgat acatgggaga
     4621 aatacagtga gcaacggcat attcgataaa aacaaccatt actcatggaa gtcattgctt
     4681 aaaaatggct tgagtaacaa tcaaaattgg ccccaagcgc ggcgcatggc caccttgcag
     4741 tcgcaatacg atgtcatcat cgttggcggt ggcggtcatg gcttggcaac cgcgtattac
     4801 ttggccaaaa accacaacgt cggcaaaatc gccgtgttgg aaaaaggcta tttgggcggc
     4861 ggcaataccg ctcgcaacac cactttggtg cgttctaact acctgtggga agacgccgcc
     4921 aatttgtacg agcattcaat gaagttgtgg gaaggcttgt ctgacgactt gaatttcaac
     4981 acttttttca gccaacgtgg cgtgtttaac ttgggtcaca atttacaaga catgcgcgac
     5041 atcgagcgtc gcgtcaatgc caataacttg cttggaatcg acgcgcatgt tttgaccgcg
     5101 caagaagtgc aagacaagat tcctttattg gattgttcac gccgttcgcg ctaccccgtt
     5161 ttgggtgcga gctggcagcc gcgcggtggc attgcccacc atgatgcggt ggctcgtggc
     5221 tttgcccgcg ccgccagcca tttgggtgtg gacttgattg aaggctgtga agtcatcggt
     5281 atggacatct ctgaaggccg cattcaaggc ttgcaaacca cgcaaggctt tgtgcgcgcc
     5341 aaccaagtcg gcgtggtcgc cgcaggccat acctcggtat tggcaggcat ggcaggctta
     5401 cgcttcccaa tagaaagcca tccattgcag gcatttgtgt ctgaatccat gcaaaaagtg
     5461 ttcgataccg tggtgatgtc caatgcggta cacggctaca tgagccaatc ggacaaaggt
     5521 gagtttgtga taggcgcagg catagatggc tatttgggct atggccaacg cggcagcccg
     5581 catgtgattg aacacacggc ggcggcgatt gtggaattgt tcccaacctt gtcacgcgtg
     5641 cgcatgaacc gccaatgggg cggcattgtg gacaccacgc ctgatgcctg tcctatcatc
     5701 agtaaaacac cggttaaggg tttgtatttc aactgcggtt ggggcacggg cggctttaaa
     5761 gccaccccag gttcgggcca tgtgtttgcc cacaccattg ccaacgacaa tccgcaccca
     5821 ttgaaccgtg gctttacctt ggaccgattc catacaggcc gcttgattga cgagcacggc
     5881 gcagccggcg tggcccacta ggagaacacc tatgctatac atccattgtc cgttttgcgc
     5941 ggaaaaacgc gatgaacaag agtttcatta cgctggcgaa gcctatattg cacggcctac
     6001 cgaccctgaa aattgcagcg atgaagagtg gggcgactat gtgttcatgc gcaccaacca
     6061 caaaggctgg atttgggagc agtgggagca tgtttcgggt tgtcgcaaag tgtttgtggc
     6121 caaacgcaac aacgtcagca acgacattga agccacttac aatttggtag aaggcaaaca
     6181 gcatttctta gaacattttg cagttgagga gtcggcatga gcgcaaaacg aattttaaaa
     6241 ccatcggtac aggtagatgg caaacaaacg ctgacttttg aatttgatgg caaaaactac
     6301 agcggtttac agggcgatac cttggcttct gccttgcttg ccaatggcgt gaaagccttg
     6361 gctcgcagct acaaatacgg ccgtttgcgc ggcatcatga gcgcgggtgc tgaagagccg
     6421 aatgctttgg tgcaaatgga aacaggcgca cattgttcac ccaacatcaa agccacccaa
     6481 gcggaattgt acgcaggcct caccgccaac cgcacttcag gctggccgag cttgcagttt
     6541 gacgtgaaag ccgtgatagg caccgtgggc aaaaacatga tggcgccggg tttttacagc
     6601 aaaaccttca aatggccgca aaaaatgtgg cctaaatacg aaaacgtcat tcgccaattt
     6661 gcaggctttg gcagcacgcc tgagcaagcc gatgccgagt ggtacgacca tttgcaccac
     6721 catgtggatg tgttggtagt tggtggtggc ttggcaggtt tacaggccgc tttaatcagt
     6781 gccaacaatg gcttgaaagt gttgttggta gacgaacaag cgcaagtggg cggttggttg
     6841 ttgtccgatg cca
//
LOCUS       DAOMVK010000064         6833 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_2997_length_6833_cov_3.155798, whole genome shotgun sequence.
ACCESSION   DAOMVK010000064 DAOMVK010000000
VERSION     DAOMVK010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 6833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6833
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2997_length_6833_cov_3.155798"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1063)
                     /gene="nusA"
                     /locus_tag="PK856_03515"
     CDS             complement(<1..1063)
                     /gene="nusA"
                     /locus_tag="PK856_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRL01475.1"
                     /translation="MSKEILTLVDALASEKNVDRETVFSALEFALATAAKKRFEQDHI
                     EVRTEINRESGEYQNYRRWLIVKDEDYTYPDVEMTEEEIEEKFPGMHLDAGEYYEEKI
                     ENVEFGRIGAQTAKQVILQRIRDAEREQILNEFLQRRENLIYGTVKRVERHGAVIEVG
                     RLEALLPRDQMIPKENLRTGDRVRAYLKEVVQQGNRPVVMLTRTSGEFLAKLFEIEVP
                     EIENDLLEIKEVARDPGFRAKIAVKANDPRIDPQGTCIGVRGSRVNAVTNELAGERID
                     VVLWSPDTAQFIINALSPADVTRILIDEDRHTVDVVVAEDQLALAIGRGGQNVRLASD
                     LTGWKINIMDAAESAQKKAE"
     gene            complement(1099..1524)
                     /gene="rimP"
                     /locus_tag="PK856_03520"
     CDS             complement(1099..1524)
                     /gene="rimP"
                     /locus_tag="PK856_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HRL01476.1"
                     /translation="MDVQTILDKTLPGLGYELVDFELTAQGDLRVFIDSPNGITVEDC
                     ATVSNHLSRVFLVEDIDYKRLEISSPGLDRPLKKAQDFARFNGQLTKLKTRLPVDNQK
                     NFLGRIIASNDDSVTLAFDGKEVTIQLSNIDKARLKPEF"
     gene            1823..2932
                     /gene="serC"
                     /locus_tag="PK856_03525"
     CDS             1823..2932
                     /gene="serC"
                     /locus_tag="PK856_03525"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HRL01477.1"
                     /translation="MTMPIIYNFSAGPAVLPESVLRQAQSEMFDYNGTGFSVMTMSHR
                     SDVFMSILYHAEQDLRQLMNIPNNYKVLFMQGGASSQFNMVTMNLARGFKRVDSVVTG
                     NWSRIAHNEMGKLTDTKIHLAAHGGELYDYTDLPAPDTWDISPDSAFVHYVINETVHG
                     LQYRDVPKPFPNQMPPMVCDMSSEILSRPINVADFGLIYAGAQKNIGPSGATIVIIRE
                     DLLDRCSNAIPDVWNYKSHVERQGMYNTPATYPIYISGLVFRWLQSQGGVASVQTINA
                     LKAKKLYEAIDSSGGFYTNKVAARARSQMNVIFHAPTPELDKLFAQESTTRGLRLLKG
                     YKSMGGMRASIYNAMPLQGVEALIDFMGEFQRRYG"
     gene            3029..3481
                     /locus_tag="PK856_03530"
     CDS             3029..3481
                     /locus_tag="PK856_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01478.1"
                     /translation="MKKAILALALLGSMGAAWAETTLPANLDKRAGEPYVDAVLKATP
                     ALKINILALLGEHGLADAWVLSGFKSGNMVVGPIKTFEASQTVSVGTFCQPHNCGDTQ
                     WTFAYQKQSQALILQRGDKVLTNAQGTKVKNNLLQILKMNAAIDQMNW"
     gene            complement(3589..3933)
                     /locus_tag="PK856_03535"
     CDS             complement(3589..3933)
                     /locus_tag="PK856_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01479.1"
                     /translation="MALKTYQVFYHFYGSDTSVDSDAPISMNVADIYSPLLGQLSEDG
                     DFIGLIDAQGETLQVMYEAEDDVYWIEVINVSQQGSHGKHMGFDELTDFFKALPEHFQ
                     PDSIDGSEFIAW"
     gene            complement(4108..4989)
                     /locus_tag="PK856_03540"
     CDS             complement(4108..4989)
                     /locus_tag="PK856_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009118274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HRL01480.1"
                     /translation="MSFLLSPIDTVETISPEEFALSYLLPRKPVVIKNLTHSWPAFQK
                     WNLAYMKDVVGDITVPLYDSAKADPAAPINAASSEMRFSDYIDLIQREPTDLRIFLFD
                     PIKYAPKLLDDYVFPTDLMGGFLDKHPNLFFGGQGSETFLHYDLDLAHIFHSHFGGRK
                     RVLLFAPEWKQRLYHMPYATYALEDYDIANPDFNRFPALKHIEGIECTLEHGDTLFMP
                     SAWWHWMQYVDCGFSISLRAWDKSWRIKAQSLWNLTVQRQFDNFMKAKYQGKYMAWKE
                     HQAWQRADKALRNHQPK"
     gene            complement(5259..5519)
                     /locus_tag="PK856_03545"
     CDS             complement(5259..5519)
                     /locus_tag="PK856_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01481.1"
                     /translation="MRTLYTILIGLALVLILRFVGNEVAGLEGQRKALVWFFPIWFVA
                     MAGNLINGMRHGYGFDEELLFFMLNFGLPCLFAAFCLHKHKT"
     gene            complement(5818..>5914)
                     /locus_tag="PK856_03550"
     CDS             complement(5818..>5914)
                     /locus_tag="PK856_03550"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003824696.1"
                     /note="catalyzes the formation of porphobilinogen from
                     5-aminolevulinate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HRL01482.1"
                     /translation="LESLMAFKRAGADGVLTYFAIEAARLLRQQN"
     assembly_gap    5915..5924
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5925..6827)
                     /gene="hemB"
                     /locus_tag="PK856_03555"
     CDS             complement(<5925..6827)
                     /gene="hemB"
                     /locus_tag="PK856_03555"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HRL01483.1"
                     /translation="MNFPHRYTPSTRMRRMRRDAFSRQLMQENTLTVKDLILPVFILE
                     GNNVEQDVTSMPGVKRLSLDKLFYTCEECVELGIPLIDLFPADLNNKSLMAEEAYNPD
                     GLVPTAIREIRARFPDLGVMTDVALDPYTTHGQDGIIDADGYVMNDITIEVLIKQSLC
                     HADAGAHIISPSDMMDGRIGAIREALEDAGHELVRIMSYSAKYASCFYGPFREAVGSA
                     GSLGKADKKTYQMDPANSNEALHEVALDIQEGADMVMIKPGMPYLDVIRRVKDEFAVP
                     TYAYQVSGEYAMLMAAIQNGWLDRD"
BASE COUNT         1770 a         1534 c         1683 g         1836 t
ORIGIN      
        1 cttcggcctt cttctgtgcc gattctgccg cgtccatgat gttgatcttc caacctgtca
       61 ggtcagaagc caaacgcacg ttctggccac cacgaccgat ggccaaggcc aattggtctt
      121 cggccaccac cacatcaaca gtatgacggt cttcatcaat caagatgcga gtcacatcag
      181 caggtgacaa ggcattgatg atgaattgag cggtatcagg tgaccacaac accacgtcaa
      241 tgcgctcgcc cgccaattca ttggtcacgg cattcacgcg tgagccacgt acaccaatac
      301 aagtgccttg tgggtctatg cgcgggtcat tggctttgac tgcaattttg gcgcggaaac
      361 ctgggtcacg agcgacttct ttgatttcca ataagtcgtt ttcaatctct ggaacttcaa
      421 tttcaaacaa tttggctaag aattcgcctg aagtacgggt taacatcacc actgggcgat
      481 tgccttgttg taccacctct ttcaagtaag cacgaacacg gtcacctgta cggaggtttt
      541 ccttaggaat catttggtcg cgcggcagca aagcttctaa gcggcccact tcaatcactg
      601 caccatggcg ctcaacacgt ttcacagtgc cgtaaatcag gttttcacgg cgttgtaaaa
      661 actcgttcaa aatttgttca cgttcggcat cacggatgcg ttgcaaaatc acttgttttg
      721 cggtttgggc accaatgcgg ccgaattcta cgttttcaat tttttcttcg taatactcac
      781 ctgcatccag atgcatgcct gggaattttt cttcgatctc ttcctcagtc atttccacat
      841 cagggtaggt atagtcttcg tctttcacaa tcaaccaacg acggtagttt tggtattccc
      901 ctgattcacg gttgatttca gtgcgcactt cgatgtggtc ttgctcgaag cgtttcttcg
      961 ccgcagtcgc caaggcaaac tccagtgcgc tgaacacggt ctcgcggtct acgttttttt
     1021 cactggccaa ggcatcgact aaagttaata tttctttgct catttttttg ttatctccaa
     1081 acgtgatatt ccgatggatt aaaattctgg cttcaatcga gccttatcga tattgcttaa
     1141 ttgtatggtg acttctttgc catcaaaagc cagtgtcaca ctgtcgtcat tgctggcgat
     1201 gatgcgaccc aagaagtttt tttgattgtc tactggcaag cgtgttttta gcttggtcaa
     1261 ttggccatta aagcgcgcaa agtcttgtgc ctttttcaaa gggcggtcta gacctgggct
     1321 ggaaatttcc aagcgtttgt aatcgatgtc ttcaaccaaa aacactcggc tcaagtggtt
     1381 gctgactgtg gcacaatctt ccacagtgat gccgtttggg ctgtcgatga aaactcgcaa
     1441 atccccttgg gcggttaatt cgaaatcaac caactcataa cctaaaccag gtaaggtctt
     1501 gtctaatatg gtttggacgt ccatttggct cccagaaata aaaaaatggg cagatgccca
     1561 tttctcgaat ttgaaaaacg tgctgatttt acccgcttta agtgggaaaa gaaagtatta
     1621 aggtgttttt cacagagaaa atcgcacaaa cttaagcttg cctctattgt tgcaacgcac
     1681 tagaatccat aaacccctca tttttggtgt tttccgtgtc gcaatcagct gaaaatactg
     1741 gggtggagag aaaagtgtga cgttacaccc gtgagggcgg cgccaagttt tgccaaccaa
     1801 gccaaataaa ggccgattca ccatgaccat gcccattatt tacaattttt ctgctggacc
     1861 tgctgtgttg ccagaatctg tgttgcgtca ggcgcaatca gagatgttcg attacaacgg
     1921 tacgggtttt tccgtaatga ccatgagcca ccgttccgat gtgttcatga gcattttgta
     1981 ccatgcagag caagatttaa gacagttaat gaacatccct aataattaca aggtgttgtt
     2041 catgcaaggg ggggcgagct cccaattcaa catggtgacc atgaatttgg ccagaggttt
     2101 taaacgggtg gattcggtgg taacgggcaa ttggagccgc atcgctcaca atgaaatggg
     2161 caaactgacc gataccaaaa tccatttggc cgcgcatggc ggcgaattat acgattacac
     2221 cgatttgcca gctcctgata cttgggatat cagcccagat tctgcgtttg tgcattacgt
     2281 gattaacgag accgtacatg gtttgcaata ccgcgacgtt cccaagcctt tcccaaatca
     2341 aatgccgccc atggtgtgcg acatgtcgag tgagattttg tcacgcccga ttaatgtggc
     2401 cgattttggt ttgatttatg cgggtgcgca gaaaaacatc ggtccatcgg gtgcgaccat
     2461 agtcatcatt cgtgaggatt tgcttgatcg ctgttctaac gcgattcccg atgtgtggaa
     2521 ttacaaatcg catgtggaac gccaaggcat gtacaacacg cccgcaacct atccgattta
     2581 catttctggt ttggtgttcc gctggttgca atcgcaaggt ggcgtggcat cggtgcagac
     2641 catcaacgct ttgaaagcga aaaaactcta cgaagccata gacagcagcg gtggttttta
     2701 caccaataag gtggctgcgc gtgcgcgttc gcaaatgaat gtgattttcc atgcaccaac
     2761 gcctgaattg gataaattgt ttgcgcaaga atccaccaca cgcggtttgc gtttgctcaa
     2821 aggctacaaa tccatgggcg gcatgcgcgc gagcatttac aatgcgatgc cattgcaagg
     2881 cgtggaagcc ttgattgact ttatgggaga attccaaagg cgctatggtt gagcaagatt
     2941 aaattcacac attgaagcgg cctgtaaact gtgtttgtgg tttacaggcc gctttttagg
     3001 ccattaacag cgattaaagg gtgacagcat gaaaaaagcc atattggcat tggcattatt
     3061 gggcagcatg ggcgcggcat gggcagaaac cacattgcct gcaaatttgg acaaacgggc
     3121 gggcgagccg tatgtcgatg cggtgttgaa ggcgactcct gccttgaaaa tcaatatttt
     3181 ggcactattg ggcgaacatg gtttggccga tgcttgggtt ttgtcgggtt tcaaatcagg
     3241 caatatggtg gtagggccga ttaaaacgtt tgaagccagt caaaccgtca gtgtgggcac
     3301 gttttgccag ccacacaatt gcggcgatac ccaatggacg tttgcctatc aaaagcaaag
     3361 tcaggcattg attttgcagc gtggcgataa ggtgttgacc aacgcgcaag gcaccaaagt
     3421 caagaacaat ttgctgcaaa tcttgaaaat gaatgcggca atagaccaaa tgaattggta
     3481 atccaatcac ggaattgcca tatgcaagcg gcctgtaaat gagttcagca tttacaggcc
     3541 gcttgcatgg tgtattgttg tgaatgacaa ggatgatgag ataacgcgtt accaagcaat
     3601 aaattcgctg ccatcaatgc tgtctggctg gaaatgttct ggcaaggctt tgaagaaatc
     3661 ggtcagttca tcaaaaccca tgtgtttgcc atgcgagcct tgttgactga catttatcac
     3721 ttcaatccaa tagacatcat cttcggcttc atacatcact tgcaaggttt caccttgtgc
     3781 gtctatcaag ccgataaaat ccccatcttc actcaattgg ccgagtaaag gtgagtagat
     3841 atcggccaca ttcatgctaa ttggcgcatc agaatcaacg gatgtatcgc tgccataaaa
     3901 atggtaaaac acttggtagg ttttgagtgc catgctgttt ccttcgttga attggtaagg
     3961 cgcacattgg atgggtgttc aagcacagaa ctgcaatgtt ttatgcggcc tgtaaggatt
     4021 tacacgcttt ctaaatgcct ccatatcatg gtttgttgaa gagtcattta taccgattga
     4081 ttggtgcgat atacaaccgt gaaacgatta cttgggttgg tggttgcgca aagccttatc
     4141 ggcccgctgc caagcctgat gttctttcca tgccatgtat ttgccttggt acttggcttt
     4201 catgaagtta tcaaattggc gctgtacggt tagattccac aaagactggg ctttgatgcg
     4261 ccaagatttg tcccaagcgc gcagcgaaat ggaaaagcca caatcgacgt attgcatcca
     4321 atgccaccaa gcgctgggca tgaacaaggt gtcgccatgt tcgagagtgc attctatgcc
     4381 ttcaatgtgc ttcagagcag ggaaacggtt gaaatcggga ttggcaatgt cgtagtcttc
     4441 taaggcataa gtggcgtatg gcatgtgata aaggcgttgt ttccattcgg gagcaaacaa
     4501 cagcacgcgt ttgcggccgc caaaatggct gtgaaagatg tgggccaaat ccaagtcgta
     4561 atgcaaaaaa gtctcagagc cttggccacc aaaaaacaaa tttgggtgtt tgtctaaaaa
     4621 tccacccatc aaatcggtgg ggaaaacgta atcatcgagc agcttgggtg cgtatttgat
     4681 gggatcaaac agaaatatgc gcaaatcggt gggttcgcgt tgaatcaaat caatgtaatc
     4741 gctgaagcgc atttcactgc tggcggcatt gatgggggcg gcagggtcag ctttggcact
     4801 gtcatacaga ggaaccgtga tgtcgcccac cacatccttc atgtaggcta ggttccattt
     4861 ttgaaaagca ggccagctat gggtcagatt tttgatgacc acgggtttgc gtggcagcaa
     4921 atacgatagg gcaaattctt cagggctgat ggtttcaaca gtgtcaatcg gacttaacaa
     4981 aaaactcatg gatggtgtgg tcaaacggtt aatctcaaga tggcatgacg atagcagaat
     5041 cgtggccaat atcaagtatt tttgctcaat aaaccgctac tttgactaag ccgtgtatgg
     5101 agcattagaa aagtttgtga caaaaggtgt taattggatt ggagtaagcc catatttgga
     5161 tgggcaagat gaaggacatg cggcctgtaa atgtgttccg ataagcatgc aatctgggaa
     5221 tctgagtcca gtaagggttg ccattttttg gaggttcgtt aggttttatg tttgtgcaga
     5281 caaaaggcgg cgaacaagca aggcaagcca aaatttagca tgaaaaacag caattcttca
     5341 tcaaagccat agccatgtcg catgccgttg attaaattgc ctgccatggc cacaaaccaa
     5401 atggggaaaa accacaccaa ggctttgcgt tgaccttcca agcccgcaac ctcattgcct
     5461 acaaagcgca gtattaatac caaagccaag ccaattaaga tggtgtacag ggttctcatg
     5521 atgctcaggc tcctccagca taaagggtgt gattcggtgg catgagtcaa aaccaaagat
     5581 ttgggattaa ccatggtgtt gctatggttt aaaaccttgc aatcgctgtg ccttaatgtg
     5641 gatttcttgg caattaatca ctggtaccat gattgaatga gtaagctgta tgggttgggt
     5701 gaagtcaaaa tcttaagcgg tctgtaaatg ggtacaggcc gcttaaaaga catgtataaa
     5761 atgactgtgg ccgataaaat ttgaggttta tcggccacag tgggttttat cagggagtca
     5821 gttttgctga cgcaacaagc gtgctgcttc aatggcaaaa taggtcaaaa ccccatcagc
     5881 gcctgcacgt ttgaaagcca tcaggctttc cagannnnnn nnnngtcgcg gtctagccag
     5941 ccgttttgaa tcgcagccat cagcatggca tattcgcccg acacttggta agcataagtc
     6001 ggtacggcaa attcgtcttt gacgcggcgt atcacatcta aatacggcat gcctggtttg
     6061 atcatgacca tatcggcgcc ttcttgtatg tccaatgcca cttcgtgtaa ggcttcgttg
     6121 ctgttggctg gatccatttg gtaggttttc ttatccgctt tgcccaagct gccagccgag
     6181 cccacggctt cacggaaggg gccgtagaag caagaagcgt atttggccga ataagacatg
     6241 atgcgcacca attcgtggcc tgcatcttcc aaagcctcgc ggatggcgcc gatgcgaccg
     6301 tccatcatgt ctgacggtga aatgatgtgt gcgcccgcat cggcgtgaca caatgattgc
     6361 ttgatcaaca cttcaatggt gatgtcgttc atcacatagc catcagcatc aatgatgccg
     6421 tcttgaccat gggtggtgta ggggtctaag gccacatcgg tcatcacacc caaatctgga
     6481 aagcgggcac ggatttcacg aatggcggtg ggtaccaaac catcgggatt gtaggcttct
     6541 tctgccatca ggcttttgtt gtttaagtcg gcggggaaca agtctatcag tggaatgccc
     6601 agttctacac actcttcaca ggtataaaac aatttgtcca agctgagtcg cttgactcct
     6661 ggcatggaag tcacgtcttg ttcgacatta ttaccctcta aaatgaagac aggcaaaatc
     6721 aaatctttaa cggtcaaagt gttttcttgc atcaattggc gtgagaatgc atcacggcgc
     6781 atgcgacgca tgcgggtgga aggtgtgtag cgatgtggga aattcatggg ttt
//
LOCUS       DAOMVK010000065         6806 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_3015_length_6806_cov_2.464672, whole genome shotgun sequence.
ACCESSION   DAOMVK010000065 DAOMVK010000000
VERSION     DAOMVK010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 6806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6806
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3015_length_6806_cov_2.464672"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PK856_03560"
     CDS             complement(<1..201)
                     /locus_tag="PK856_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HRL01484.1"
                     /translation="MKKTTVALSLLSLMLALPSHAQTARKPSSFEAGKKEAATKIKTQ
                     TAALHKLGSKRDFDQMARVYEQD"
     gene            390..917
                     /locus_tag="PK856_03565"
     CDS             390..917
                     /locus_tag="PK856_03565"
                     /inference="COORDINATES: protein motif:HMM:NF027567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRL01485.1"
                     /translation="MATVHPRLMMWHLMNETRVAMVSFRKLDGRNQAQPMTLFNKEIP
                     KSVLVEQHLEEQHQLYFFVRTDSELCRSVEAGHSELGVTFCKHSADLYAYIEGRASIS
                     NDRKQIEQLWTPTAEAWFNGYQDPQLRLLLVHGESADVWHNTHSATQQWWHITKKVVG
                     AGDEAPLNTKDHLQL"
     gene            complement(1001..2371)
                     /locus_tag="PK856_03570"
     CDS             complement(1001..2371)
                     /locus_tag="PK856_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL01486.1"
                     /translation="MKINVQDFFNQHRLSSQQWIIFGFTFLVALFDGLDTAAIGYIAP
                     SLLTEWNLEKSHLAPVMSAALFGLAVGAVSFGPVADKIGRKTVLIIAVFIFSTCSIAS
                     AFAQNLEQLEILRFITGVGLGAAMPNAVTLLSEFCPDNKRSLLVNTMFCGFPVGAAVG
                     GFMSAWLIPQFGWQSVLIVGGTMPLILCVLMVFMLPESVRYMVIKNKPTAQIKSILAK
                     IDASANQAGEFVLTEKAAVNTNQKSGIAIVLSKHYLLGSVMLWASYFLGLMVFYAMIN
                     WMPILFKEANMPKELGPIVAGLFALGGLGAIANGWLMDRFNGNLLIAAFSFLTAVSVA
                     FIGLSLGWGLAAFIAVVIFAGIMQNTAQSSLPALAANFYPTAGRTTGVSWMTGIGRFG
                     GIAGSFLVAYMVEKHMGLVEIFYILAIPSIIMTVCLLVKNALYKNEAERMRNKDTETV
                     RHTPSH"
     gene            complement(2636..3310)
                     /locus_tag="PK856_03575"
     CDS             complement(2636..3310)
                     /locus_tag="PK856_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01487.1"
                     /translation="MKPIACLFALPFLLATPLALAESPAINDVVAQTQGDLDGDGIAE
                     YVEIRNTAANSELDGNGFQRALFVYKVKNGQKTLWQRSDTAVYASNAGGMMGDPFEDL
                     SIKKGSLFLTQSGGSSWKWGFTDQYRFQNGQFELIGYQSTSGRLCDYWEFVDANLSTG
                     KIVYRKEFDHDKECGNDENKGKKDIKETFVNKKIALNFANRHKQDVKIIAPKSKETIY
                     IATSSS"
     gene            complement(3666..6215)
                     /locus_tag="PK856_03580"
     CDS             complement(3666..6215)
                     /locus_tag="PK856_03580"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRL01488.1"
                     /translation="MSENLLPDSAPKPQPSIAETKLVQASKGNGKSDEALSLEQAMHD
                     NLAAMHKVASLVPSLRVLIGLSISTLVILGLYFGQDLLIPLALATLFGFLLNPAVSWL
                     KNKGLPRMLSILVVISVALGILIAGAAYLGMQLGSLSQELPTYQTTIRSKLQNLKTFS
                     EGPSVWDGAISTFTVIKQTLSSEPTPKQNHGNNLPSAQKKASAKETTKQDSNNTSTAN
                     DTSPVQEVRLSQNEHGPIDDVIMWLRAIANPIATAGIVLLFVVLILLDRSDLRDRILR
                     LLGNNLNTATDTLDEAAERIGTYLRMQLIVNFSYGIPMALGLWFIGVPAAVLWGMVAV
                     VMRFVPYVGPMLSSIFPLLLAFAVDPSWNMLLWTLGLIVALELISNNIVEPLLYGSST
                     GLSTLSIILAATFWTALWGPIGLILSTPLTACLLVLAHYIPALKFFEILLGNTPVLDA
                     PQRFYQRLLADNAEEALELAHADIEQNLPNDADETIMARKITAFYDGVSLPAMRLFSS
                     LHNDTATAEHRLRINNGLKQFTQEMRDEYPATVIPQHAYPRVLCVAARWEVDSKAADM
                     LVHSFQLQSYAAQAWPNPLLQHLSSIDQAQWQDFDVVCVSVFNPQPGAALRLICRHLR
                     KRWPHLRIMLATWNADTHKISASFIERYGVDCVVDNMQALGLHLDKIRLNSNESTAAS
                     APLPRNEDERVSALDKDKLLAPTLMPLYQEQIQQARSAFDTAYAQISWVDSDWVYTPA
                     SSLLPPDEQTAEAGVPREHTVCQYLIQQNSALVIEDTKRDPRFAEQQEFDQRVRFYAG
                     VPLRDEDGMVLGSLCVMDDKPRQISEEDLDLLQEMADDLMLALREQNHNGD"
     gene            6406..>6806
                     /gene="azu"
                     /locus_tag="PK856_03585"
     CDS             6406..>6806
                     /gene="azu"
                     /locus_tag="PK856_03585"
                     /inference="COORDINATES: protein motif:HMM:NF012355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HRL01489.1"
                     /translation="MNAKLKWMLLGSCLSLASVSVHAACSVNLTGNDAMQLSSKQIVV
                     PKTCSDFTIHYKNIGKLPKASMGHNVVVSKLSDMSGVLRDGMKAGLAQDYVKAGDSRV
                     LAKTRLLGGGESASLTFKVAKLQDGAYGFVCT"
BASE COUNT         1792 a         1548 c         1738 g         1728 t
ORIGIN      
        1 gtcttgctcg tatacccgcg ccatttggtc gaaatcccgt ttgctgccaa gtttgtgcag
       61 ggcggcggtt tgggttttga ttttggtggc cgcctctttt ttgcctgctt caaaagatga
      121 gggtttgcgt gcggtttgcg cgtggctggg caatgccaac atcaggctta acaggcttaa
      181 ggcaacagtg gtttttttca tggctgtctt tgcaaagagg ggttgcggcc attttaatga
      241 tggcgcatcg aaaaatcgag tgtagactgt cttggtgctg agttcatgaa tacagtagca
      301 gtttttactc aatttgagcg cattttgtgg aaccgatgca atttgcattt tgactaagta
      361 agaccgatac ctgtttgtag gagaacacca tggcaaccgt gcatcctcga ttaatgatgt
      421 ggcatttgat gaatgaaacc cgcgtggcga tggtttcttt tcgcaagctt gatggccgca
      481 accaagcgca gcccatgact ttgtttaaca aagaaattcc caaaagcgtg ttggtagagc
      541 agcatttgga agaacaacac caattgtatt tctttgttcg aaccgacagt gaattgtgcc
      601 gcagtgtgga agcgggtcat agcgaattgg gcgtgacgtt ttgcaaacac agcgccgatt
      661 tgtatgctta tattgaaggc cgcgccagca tcagcaatga cagaaaacag atagaacaac
      721 tgtggacgcc aactgctgaa gcttggttta acggctacca agacccgcaa ttgcgtttgt
      781 tactggtgca tggcgaaagc gccgatgtct ggcacaacac ccactccgcc acgcaacaat
      841 ggtggcacat caccaaaaaa gtcgtgggcg cgggtgatga agccccgttg aataccaaag
      901 accatttgca gttgtaattg aaacattgtt tcatgcgcca taaaacaagc ggcctgtaaa
      961 ccttgtgaga ggatttacag gccgcttttg tcactgtgag ttagtggcta ggagtatggc
     1021 gcactgtttc tgtgtctttg ttgcgcatgc gttctgcttc attcttatac agggcatttt
     1081 tcaccaatag gcacaccgtc atgatgattg aagggattgc caagatatag aaaatctcaa
     1141 ccaagcccat gtgtttttcc accatatagg ccacaagaaa cgagcctgca attccgccaa
     1201 agcggcctat gccagtcatc catgacacgc ctgtggtgcg gcctgcggta gggtagaaat
     1261 tggcggccaa agctggcaat gacgattggg cggtgttttg catgatgccc gcaaaaatca
     1321 ccacagcgat gaaggcagcc aagccccaac ccaaagacaa accgatgaac gccactgaaa
     1381 ctgcggttaa gaagctgaat gctgcaatca gtaagttgcc attgaatctg tccatcagcc
     1441 agccattggc aatcgcaccc aagccaccca aggcaaacaa gccagccact ataggaccta
     1501 attctttggg catattggct tctttaaata aaatgggcat ccagttaatc atggcgtaaa
     1561 acaccatcaa acccaagaag taagatgccc acaacatcac cgaacccaat aaatagtgtt
     1621 tggacaacac gatggcaatg ccgctttttt gattggtgtt cacggctgct ttttcggtta
     1681 atacaaattc gccggcttgg ttggcactgg catcgatttt ggccaaaatg cttttgattt
     1741 gggctgtggg tttgttttta atcaccatgt agcgcacgga ttcaggcaac atgaacacca
     1801 tcaacacaca caaaatcaaa ggcatggtgc cgcccacgat caaaacgctt tgccagccaa
     1861 attgtggaat caaccatgcc gacataaagc cgccaactgc cgcgcccaca gggaagccac
     1921 agaacatggt gttcaccagc aaagagcgct tgttatcagg gcaaaactca gacaataagg
     1981 tcaccgcatt gggcatggca gcgcccaaac ccacgcccgt aatgaagcgc aagatttcca
     2041 attgttccaa attttgggca aatgctgagg cgatgctgca agtggagaaa atgaataccg
     2101 caatgattaa aacggttttg cggccgattt tgtcggcaac agggccaaac gataccgcac
     2161 cgactgccaa gccaaacaag gcagcactca taacgggggc caaatggctt ttttcgagat
     2221 tccattcggt caataaactc ggtgcaatgt aaccgattgc cgcggtgtcc aagccatcaa
     2281 acaatgcgac caaaaacgta aagccaaata tgatccattg ttgagaggac aggcggtgct
     2341 gattaaagaa atcctgtacg ttgatcttca tagtgttctc ttctggtgat tgggggccac
     2401 ctcattatgg atgtgtgctg agtttgctca gcttagtatg gcgaggtgat gcttcatcat
     2461 attccgatta atgtgcaaat ttgtagcgga aaacgagact aagtgatatg accagaatgc
     2521 atagctgatg agggttagta ttgcaaaata aaaccacata ttgataaggc caagaaatgt
     2581 ttacaggccg cttaagtggc ggcctgtaat agggtggctt tcaaacgctt gaaagtcaag
     2641 aggatgaggt ggcgatgtaa atggtttctt tgcttttcgg ggcgatgatt ttgacatctt
     2701 gtttgtggcg attggcaaaa ttcaaggcaa tctttttgtt gacaaaggtt tctttgatgt
     2761 cttttttgcc tttgttctca tcgttgccac attctttgtc gtgatcaaac tctttgcggt
     2821 aaacgatttt tcctgtgctg aggttggcat cgacaaactc ccaataatcg cacaaacggc
     2881 cagaagtgga ttggtagcct atcagttcaa actgtccgtt ttgaaagcgg tattggtcgg
     2941 taaatcccca tttccaagag ctgccaccgc tttgggttaa aaacaaactg cctttcttga
     3001 tgcttaagtc ttcaaatggg tctcccatca tgccgccagc attgcttgca taaacggccg
     3061 tgtctgagcg ttgccacagg gttttttgac catttttgac tttgtacacg aataaggcac
     3121 gctgaaatcc gtttccatcc aattcagaat tggcagcggt attgcggatt tccacatact
     3181 cagcaatacc gtctccatcc aaatcgcctt gagtctgtgc caccacgtca ttgatggcag
     3241 gactttcggc taaggcaagt ggcgtggcca ataaaaatgg cagggcaaag aggcaagcaa
     3301 tcggtttcat ggtgaggctc ctgtgggttg tggagtcagt atacgaaaaa cggcccaaaa
     3361 gaaaacgtct attttgaaaa gtacaccttg ttgcgctgtc agtgtgcatg ttttgcccta
     3421 aatgcagttg aaacagttgt ggggcagtga aagggtagtt ggcaagcgtg cggtaagtgc
     3481 gaggtgttgt gtagagcaag gagacagact tgggtacaca tgaggactaa tgcaccatca
     3541 gactttgtga ctgtttgtga ctgattggat atgagttgaa gcaatggaca gatgccatga
     3601 aactatgcag cctgtaaacc ctttgcgagc ttacaggccg cttgtgtcaa taatcaggct
     3661 tgtgcttaat ccccgttgtg attttgttca cgcaaagcaa gcatcaaatc gtcggccatt
     3721 tcttgcagta aatccaaatc ttcttcactg atttgacggg gtttgtcatc catcacacac
     3781 aggctgccca ataccattcc atcctcatct cgcaaaggca cgcccgcata aaaacgcaca
     3841 cgttggtcaa actcttgttg ctctgcaaac cgagggtcac gtttggtgtc ttctatcacc
     3901 aaggcgctgt tttgctgaat caaatattgg cacacagtgt gttcacgggg cacgccagcc
     3961 tctgcagttt gttcatcggg cggcaataag gagctggcag gggtgtagac ccaatcgctg
     4021 tctacccaag aaatttgggc ataagcggtg tcaaacgcac tgcgtgcttg ttgtatttgt
     4081 tcttggtaga ggggcattaa tgtcggtgcc aataatttgt ctttatccaa cgccgatacg
     4141 cgttcatcct cattgcgtgg caaaggagca ctggctgccg ttgattcgtt gctgtttaag
     4201 cgaattttat ccaaatgcaa tcccaaggct tgcatattgt ccaccacgca gtctacccca
     4261 tagcgctcaa tgaatgaggc gctgattttg tgggtgtctg cattccaagt ggccaacatg
     4321 atgcgcaaat gtggccagcg tttgcgcagg tgacgacaaa tcaaacgcaa ggctgcgcct
     4381 ggttgtggat tgaatacgga cacacaaacc acatcaaaat cttgccactg ggcttggtct
     4441 atgctgctta agtgctgcaa caatgggtta ggccatgcct gtgcggcata agattgtaat
     4501 tgaaagctgt gtaccaacat gtccgccgct ttgctatcga cttcccaacg agccgccaca
     4561 cacaaaacac ggggatacgc atgttgagga atgacagtcg cgggatactc atcacgcatt
     4621 tcttgggtga attgtttcag gccgttgttg atgcgcaaac ggtgttctgc ggtggcggta
     4681 tcgttgtgca aactggaaaa caaacgcatg gcaggcaggc tgacgccatc gtaaaaagcc
     4741 gtgattttgc gcgccataat ggtttcatct gcatcattgg gcagattttg ctcaatgtct
     4801 gcgtgtgcaa gttccaatgc ttcttcggca ttgtcggcca gcaagcgctg gtaaaagcgt
     4861 tgaggggcat ccaacacagg ggtgttaccc aacaaaattt caaagaattt taaggcagga
     4921 atgtaatgcg ccaaaaccag caaacaagcg gtgaggggcg tggacagtat caagccaatg
     4981 gggccccata aagcggtcca aaatgtggcg gccaaaataa tggaaagcgt tgaaagcccc
     5041 gtgctgctgc catacagtaa aggctcaacg atattgttgc tgatgagttc cagggcaaca
     5101 atcaaaccca gagtccacaa cagcatgttc caagaggggt ctacggcaaa agccaacagc
     5161 agtggaaaaa tagacgacaa catcggcccc acatatggca caaaacgcat caccaccgcg
     5221 accatgcccc aaagcactgc agcaggcacg ccaatgaacc acaatcccaa agccatgggt
     5281 atgccatagc tgaaattaac aatcaattgc atgcgcaggt atgtcccaat gcgctcagcg
     5341 gcttcatcca gtgtgtcggt ggccgtattc aaattgttgc ccaagagccg caaaatgcga
     5401 tcacgcaaat cactgcgatc aagcaagatt aacacgacaa acaataatac gatgcctgcc
     5461 gtggcgatgg ggttggcaat tgcgcgcagc cacatgatga catcgtcgat ggggccgtgt
     5521 tcattttgac tcagcctcac ttcttgaacg ggtgaggtgt cattggctgt actggtgtta
     5581 ttgctgtctt gtttggttgt ttctttggcg ctggcttttt tttgagcaga ggggaggttg
     5641 ttgccatggt tttgcttggg tgtgggttca ctggagagcg tttgtttgat aaccgtgaac
     5701 gtgctgatgg caccatccca cacgctgggt ccttcagaga aggttttgag gttctgtaat
     5761 ttgctgcgta tggtggtttg gtatgtgggc aattcttgac tcaagctgcc caattgcatg
     5821 cccaaatacg cggcaccagc aatcaaaatg cccaaagcca cgctgatgac caccaaaata
     5881 gacagcatgc gtggcaagcc tttgtttttc agccaagaca ctgcgggatt caacaaaaag
     5941 ccaaacaaag tggccaaggc gagtgggata agcaaatctt ggccgaaata caggcctagt
     6001 atgaccaagg tactgatgct caagccgatg agtaccctca agctgggcac caaactggcc
     6061 actttgtgca tggcggccag attgtcgtgc atggcttgtt ctaagctgag cgcctcatca
     6121 gacttaccat tgcctttgct cgcttgaaca agcttagttt cagcgatgct gggttgaggt
     6181 tttggggctg agtcgggaag caaattttcg ctcatggttg gctttcatgt ggaaagactt
     6241 gggttagggt acaaatccag caagatatgc accagtaata tcagaatgcg tgcagagcca
     6301 aaagttcgtt gcaagcttat gaggctgagt aaaaggcttg actttgatta tttgttatag
     6361 taatttctgt aataacagaa attactataa cttaaaggat gcaaaatgaa cgcgaaactc
     6421 aaatggatgt tactgggctc atgcctcagt ttggccagtg tgtctgtcca tgcggcctgt
     6481 agtgtgaacc tcacaggcaa cgatgcgatg caattgagca gcaagcagat agtggtgccc
     6541 aaaacctgta gtgatttcac catccattac aaaaacatcg gcaaattgcc caaggccagc
     6601 atgggccaca atgtggtggt gagcaaactc agtgacatga gtggggtatt gcgcgatggc
     6661 atgaaggcgg gcttggcgca agattatgtt aaagcaggcg acagtcgtgt gctggcgaaa
     6721 acccgtttat tgggtggtgg cgagtcggcc agtctgacgt ttaaggtggc aaaattacaa
     6781 gatggggctt atggctttgt ctgcac
//
LOCUS       DAOMVK010000066         6749 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_3041_length_6749_cov_3.268151, whole genome shotgun sequence.
ACCESSION   DAOMVK010000066 DAOMVK010000000
VERSION     DAOMVK010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6749
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3041_length_6749_cov_3.268151"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PK856_03590"
     CDS             complement(<1..224)
                     /locus_tag="PK856_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRL01490.1"
                     /translation="MSMQTLVRGPVMADVVAFELTDVEKARLMRPEIGGVILFRRNFE
                     SVKQLKALVAEIKALRTPELIIAVDHEGGRV"
     gene            complement(283..1794)
                     /locus_tag="PK856_03595"
     CDS             complement(283..1794)
                     /locus_tag="PK856_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HRL01491.1"
                     /translation="MNKITIMAAVCITAFGLSACDKPDWLSKKDDRNNTEYVQEVKPA
                     DSESVAMLLPDFTKLVTEQGPTVVSIQATKSETQAQSYEGTSGEEDPMLEYFRRMIPN
                     FPNTPNSPSNPENPDEEDGASFGSGFIISADGYILTNTHVVTGSDSIKVVLADKREFE
                     AKLVGSDTQSDVSLLKIDAKDLPVIKLGQPDDLRVGEWVAAIGAPFGFDNTVTAGIVS
                     AKGRSLPNENYTPFIQTDVAINPGNSGGPLFNLKGEVIGINSQIYSRSGGFMGISFAI
                     PIDVATNVAEQLKKYGRVRRAQLGVIIQEVSYDLAKSFKMDKPSGALVVRVLPNGPAA
                     NAKLQVRDIVLKVNGEAIQRSSDLPVLIGAMQPGTKIELTVWRDGKEVVLPVELTELT
                     GESAIPTPEASGGGNADTSVRINVPEVGLMLTPLSATEATQREINGGLVVLDAQGLAA
                     ASGLRRGDVIISAGPNPITDEKTFKQALSEATTAAPLFVMRGQDTFFLPLRVK"
     gene            complement(1923..2555)
                     /gene="nth"
                     /locus_tag="PK856_03600"
     CDS             complement(1923..2555)
                     /gene="nth"
                     /locus_tag="PK856_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRL01492.1"
                     /translation="MNQYIRHEIFSRLQAANPHPTTELVFGDAFQLLIAVLLSAQTTD
                     VAVNKVTAVLFALAPTPQEMLSLGEETVRDTIKTIGLYRTKATHVIATCEMLLNNFGG
                     EVPQTRAELESLPGVGRKTANVVLNTAFKQPAMAVDTHIFRVSNRTGIAKGKNVREVE
                     DKLVKYIPKEFLLDAHHWLILHGRYTCKALKPQCAQCIIEDLCEYKAKAL"
     gene            complement(2552..3019)
                     /locus_tag="PK856_03605"
     CDS             complement(2552..3019)
                     /locus_tag="PK856_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HRL01493.1"
                     /translation="MSDYSKRNLKQYQGKVLRISDGDTLQIRDEHGQKQRVRLAFIDA
                     PETEQAGGRDSLRQLQNLLENQKVAVQVVDVDNYKRQVAIVWLQNQDINATQIATGMA
                     WQYASIAKRQQTPQGYQYYHCLEQSARNSKQGLWRNAKAQAPWEYRQQRRQAQ"
     gene            complement(3165..3719)
                     /locus_tag="PK856_03610"
     CDS             complement(3165..3719)
                     /locus_tag="PK856_03610"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRL01494.1"
                     /translation="MPYRLTQIITKTGDSGTTGLGDGSRVPKSSLRIIAIGEVDELNA
                     HLGVLLTMNLPDDIRAVLTKIQHQLFDLGSELAVPGWSLIDDTYLQFLEQAAATWHAQ
                     LTPLKEFILPGGSVAAAQVHVVRTVARRAERSVVALQDAAVAVSGNVQKYLNRLSDVM
                     FILAREVNRREGVSDVLWQKNQTE"
     gene            complement(3790..4464)
                     /gene="rpiA"
                     /locus_tag="PK856_03615"
     CDS             complement(3790..4464)
                     /gene="rpiA"
                     /locus_tag="PK856_03615"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009119346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HRL01495.1"
                     /translation="MTTQNDLKRQAARRALDYLPNDDYIGVGSGSTVSLFIEELVAKK
                     DRIRGVVAASLESRELLEKLGINVVALADVGKLPVYFDGADEVNHSLQMIKGGGAALL
                     GEKIVAHASRQFICMADESKYVSKLGKFPLPVEVVPFARSWVASQLVKLGGRPVWREG
                     VITRGGHEILDVHDLSIMEPMKLEDEINRIPGVMENGVFARHPADVLVLGHPDRAEII
                     TVGGAK"
     gene            complement(4519..4998)
                     /gene="ispF"
                     /locus_tag="PK856_03620"
     CDS             complement(4519..4998)
                     /gene="ispF"
                     /locus_tag="PK856_03620"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HRL01496.1"
                     /translation="MKIRVGQGYDVHQLVVGRPLILGGVTIAHDKGLLGHSDADQQGR
                     LRPGQQAGAAALGDIGHHFPDTATEFKGADSRVLLRAAYQSVQAQGWKVVNVDTTIIA
                     QQPKLKPHLLGMRENIAADLGLSVNEVNIKGKTNEKLGYLGREEGIEAQAVVLLVSA"
     gene            complement(5019..5726)
                     /gene="ispD"
                     /locus_tag="PK856_03625"
     CDS             complement(5019..5726)
                     /gene="ispD"
                     /locus_tag="PK856_03625"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HRL01497.1"
                     /translation="MTQRFFAVVPAAGVGSRFGSQIPKQYTEIAGQAVLLHTVQLLLA
                     NETLAGVAVVLSPDDGYFKDLNLPAWAQTERLQVLYCGGDSRAESVRNGINALMAQQL
                     VQNEDWVLVHDAARCCLPQAALQRLLAAAEGDCNGAILALPVADTLKKQALTGRIDHT
                     VDRTGLWQAQTPQMFRAAALQAALTQVDLQHITDEASAMEAQGVFAQLVQGDVRNLKL
                     TLAQDATLVELLLRHAD"
     gene            complement(5723..6451)
                     /gene="dnaQ"
                     /locus_tag="PK856_03630"
     CDS             complement(5723..6451)
                     /gene="dnaQ"
                     /locus_tag="PK856_03630"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003783195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HRL01498.1"
                     /translation="MAVRQIVLDTETTGLYANQGDRLIEFAGLEMIDRRLTGEYLHVY
                     VHPERGIAEEAIAVHGITLDMLADKPKFAGVATEIADFLRDSELIIHNAAFDVGFLDM
                     EFGRLGMPKLDTVVSKVTDTVAMARKLYPGQKVNLDALCNRLGVDKTKRVLHGALIDC
                     ELLAEVYLGMTRGQFSLDVKSADDGKDGGQALGQFKRPVNMSFKVIPMDEDETAAHEA
                     YVKVLDKAVGGESLYRQQMHESQA"
     gene            complement(6460..>6749)
                     /locus_tag="PK856_03635"
     CDS             complement(6460..>6749)
                     /locus_tag="PK856_03635"
                     /inference="COORDINATES: protein motif:HMM:NF017870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HRL01499.1"
                     /translation="VDPDVENPPSMEEVRDTARMLVRSTINNMASAGYLTQQNGIVDS
                     EMVVKNNELLLNGKRFETAPQQNIDDLMDEGAAAASEEQAASAPVAASAVK"
BASE COUNT         1615 a         1880 c         1508 g         1746 t
ORIGIN      
        1 acacgaccgc cctcatggtc taccgcaata atcaattcag gcgtgcgcaa ggctttgatt
       61 tcagccacca aggcttttaa ttgtttcacc gattcaaaat tgcggcgaaa caaaatcacg
      121 ccaccgattt caggccgcat caaacgggct ttttcaacat cggtcaactc aaaagccacc
      181 acatcggcca tcacagggcc acgaactaag gtttgcatac tcatctaatc cttaaaataa
      241 caacaggcag cggttttggc gctgcctgtt cacattcact acttacttga cacgcagtgg
      301 taagaagaag gtgtcttgac cgcgcatcac aaacaatggc gccgccgtgg tggcctcgct
      361 cagagcttgt ttgaaggttt tttcatcggt aatcgggtta ggacccgcgg agatgatgac
      421 gtcgccacga cgcaagcctg atgctgccgc cagaccttgg gcatccagca ccaccaaacc
      481 accattgatt tctcgttgtg tggcttctgt ggctgacaat ggggtcaaca tcagacccac
      541 ttcaggcaca ttgatgcgaa cgctggtatc ggcattacca ccaccactag cttcaggcgt
      601 tggtatcgcg ctttcaccgg ttaactccgt caattccact ggcaacacca cttctttgcc
      661 atcgcgccat accgtcagtt caattttggt acctggttgc atcgcaccta tcaacacagg
      721 caaatcactt gaacgctgaa tggcttcacc attcactttc aatacaatgt cacgcacttg
      781 caatttggca ttcgcagcag ggccgtttgg caagacacgc acgaccaaag ctccacttgg
      841 tttgtccatt ttgaaagact tggccaaatc gtaagacact tcttggatga tcacgcccaa
      901 ttgagcacgg cgcacacggc catatttttt caattgttcg gcaacattgg tggccacatc
      961 aatcggaatc gcaaacgaga tgcccataaa accacctgag cgactgtaaa tctgcgagtt
     1021 aatcccaatg acttcacctt tcaaattgaa caacgggccg cccgagttac cagggttaat
     1081 cgccacatcg gtttggatga aaggggtgta gttttcattg ggcaatgagc ggccttttgc
     1141 cgacacgatg cctgcagtca cggtgttatc aaagccaaac ggtgcaccaa tggctgctac
     1201 ccactcacct acgcgcaaat catcaggttg gcctaatttg atcacgggca aatctttggc
     1261 atcgattttc aataacgaca catctgattg cgtgtctgaa cctaccaatt tggcttcaaa
     1321 ttcacgtttg tcagccaaca ccactttgat ggaatcactg cccgtcacca catgggtatt
     1381 ggtcaaaatg tatccgtcgg cactgatgat gaagcctgaa ccaaaactgg cgccatcttc
     1441 ctcatctgga ttttctggat tagacggtga gttaggcgta ttcgggaaat taggaatcat
     1501 gcgacgaaag tattccaaca taggatcttc ttcgccactg gtgccctcat acgattgggc
     1561 ctgagtctcc gatttggtag cctgtatgct gacaactgta ggaccttgct ccgtcaccaa
     1621 cttggtaaaa tcaggtaaca acatcgccac cgattcgctg tcggcaggct ttacctcttg
     1681 tacgtattcg gtattgttgc ggtcatcttt tttgcttaac caatcgggtt tatcacaagc
     1741 actcaagcca aaagcagtga tgcacacggc ggccattatc gtgattttgt tcactcagaa
     1801 aatccttttt tgactacttt gtcgtccatc ttataggggg catttaccaa gttatcatca
     1861 tattggtaaa actgtgaaat tatggcattt tttgctgcaa ttgcacacgc aaatttacgc
     1921 attcataagg cttttgcctt atattcacac aaatcttcaa tgatgcactg agcgcactgg
     1981 ggttttaagg ctttacaagt gtaacgacca tgtaaaatca accagtgatg cgcatccagt
     2041 aaaaattcct ttggaatata ttttaccaat ttatcttcca cttcccgcac atttttaccc
     2101 ttggcaatgc cagtgcggtt agacacgcgg aaaatgtgtg tgtctacggc catggcaggc
     2161 tgtttgaatg cggtgttgag caccacattg gcggtttttc tgcccacccc aggcaagctt
     2221 tccaattcag ctcgggtttg cggcacttcc ccaccaaagt tattgagcag catctcacat
     2281 gtggcgatga catgggtggc cttggtgcga taaagtccga tggttttaat ggtgtcacgc
     2341 actgtctctt cgcccagtga cagcatttcc tgtggagtcg gtgccaaggc aaacaacacc
     2401 gccgtgacct tgttcaccgc cacgtctgtg gtttgtgctg acaacaacac cgcaatcaac
     2461 aattggaatg catccccaaa caccaactcg gttgttgggt gtggattagc ggcctgtaaa
     2521 cgactgaaga tttcatgacg tatgtattgg ttcattgtgc ttgcctgcgc tgttggcgat
     2581 attcccacgg tgcttgagcc ttggcattgc gccacaagcc ttgcttgctg ttgcgagcgc
     2641 tttgctccag acaatggtaa tactgatagc cttgaggggt ttgctgacgc ttggcaatgg
     2701 aagcatattg ccatgccatg cccgtggcaa tttgcgtggc attgatgtct tggttttgca
     2761 accacacgat ggccacttga cgcttgtaat tgtcaacatc caccacttgc acagcaactt
     2821 tttggttttc caacaggttt tgcaattgac gcaagctgtc tcgcccacct gcttgctcgg
     2881 tttcaggtgc atcaataaat gccaaacgca cccgctgctt ttgcccatgc tcatcgcgaa
     2941 tttgcaaggt atcgccgtcg ctgatgcgca agactttccc ttggtattgt ttcaaattgc
     3001 gtttggaata gtcgctcatt tgcgcgggtt gttggccaca ttgactggga ttaaccgcta
     3061 ccggcggtgt tttcccacaa gcactttgac ttaacaatat ccccaaacac agggtactta
     3121 aaaacagtgt attcacaact cagactttgc tctgtaacaa cagattattc ggtttggttt
     3181 ttttgccaca agacatcgct cacaccttcg cggcgattca cctcacgtgc caaaataaac
     3241 atcacatcac tcaagcggtt caggtatttt tgcacattgc ctgacacggc cacagcagcg
     3301 tcctgtaaag ccaccacact gcgctcagcg cgccttgcta cggtacgcac cacatgcact
     3361 tgcgcagcag ccaccgaacc acctggtaaa ataaactctt ttaaaggcgt gagctgagca
     3421 tgccatgtgg ccgcggcttg ttccaaaaat tgcaaataag tgtcatcaat caagctccaa
     3481 cctggcacag ccagctcaga acccaaatca aacaattggt gttgaatctt ggttaacacg
     3541 gcgcgaatgt catcgggcaa gttcatggtt aacaacacgc ccaaatgggc attgagctca
     3601 tccacctcgc caatggcgat gatgcgcaaa ctggacttgg gcacacgcga accgtcgccc
     3661 aatcctgtgg taccagagtc acctgttttg gtgatgattt gtgtcaaacg gtatggcatg
     3721 tttttcctta caaaattaaa aagccgatgg tgccttacag cccatcggct ccatctgatg
     3781 cggcctgtat tatttggcgc cgcctacggt gatgatttca gcacggtcag gatgacccaa
     3841 taccaacaca tcagcaggat ggcgggcaaa cacgccattt tccatcacgc cagggatgcg
     3901 gttgatttca tcttccaatt tcatgggttc catgatgctc aaatcatgca catccaaaat
     3961 ctcatgtccg ccacgggtaa tcacgccttc acgccacaca ggacggccac ctaatttcac
     4021 caattgtgac gccacccaag agcgtgcaaa cggcaccact tcaacgggca atgggaattt
     4081 acccaattta gacacgtatt tgctttcatc ggccatgcaa atgaattggc gtgaagcgtg
     4141 ggccacgatt ttttcgccca acaaagccgc accaccgcct ttaatcattt gtaatgagtg
     4201 gttcacttcg tctgcgccgt caaaatacac aggcaatttg cccacatcgg ccaaggccac
     4261 cacattgatg cccaattttt ccaacaattc acgcgattcc aatgaggccg caaccacacc
     4321 gcgaatgcgg tcttttttcg ccaccaattc ttcaataaac aaagacacag tagagcccga
     4381 acccacgccg atgtaatcat cgtttggcaa gtaatccaat gctcggcgag ccgcttgtcg
     4441 ttttaaatca ttttgagtag tcattttgtc tttccttctt gtggggtggg cgtttgactc
     4501 agttcacagt gtaaggtttt aggcgctgac aagcaacacc acagcctgcg cttctatgcc
     4561 ttcttcacgc cccaaatagc ccagtttttc gttggttttg cccttgatat tgacctcgtt
     4621 cacgctcaag cccaaatcgg cagcaatgtt ttcacgcatg cccagcaaat gcggcttgag
     4681 cttgggctgt tgcgcgatga tggtggtgtc cacattcacc actttccaac cttgcgcttg
     4741 cacgctttgg taagccgccc gcaacaacac gcggctgtct gcgcctttaa attctgtagc
     4801 ggtatcgggg aaatggtggc caatatcgcc caaagccgcc gcgccagctt gttggccagg
     4861 gcgcaggcgg ccttgttgat cggcatcgga atgccccaac aagcctttgt catgcgcgat
     4921 ggtcacacca cccaaaatta aaggtcggcc taccaccaat tggtgcacat catagccttg
     4981 gcccactcgt attttcattt gtcttcctag gtattctatc aatcagcgtg gcgcaacagc
     5041 agctctacca aggtcgcgtc ttgtgccaaa gtcagcttca aattgcgcac atcgccttgc
     5101 accaattgtg caaacacgcc ttgggcttcc atcgccgatg cttcatcggt gatgtgttgc
     5161 aaatccactt gggtcaatgc ggcctgtaaa gccgccgcac ggaacatttg tggcgtttgc
     5221 gcttgccaca aacctgtgcg gtctacggta tggtctatgc ggcctgtaag cgcttgcttc
     5281 ttcaaagtat cggccacagg caaagccaaa atcgccccat tgcaatcgcc ctctgccgca
     5341 gccagcaggc gctgcaaagc cgcttgaggc aaacagcaac gtgccgcatc gtgcaccaac
     5401 acccaatcct cattttgtac caactgctgc gccatcaagg cattgatgcc attgcgcacg
     5461 ctttcggcac ggctgtcgcc accacaatac aacacttgca aacgctcggt ttgcgcccat
     5521 gcaggcaagt tcaagtcctt aaaatagcca tcgtcaggcg acaacaccac tgccacaccc
     5581 gccagtgttt cattggccaa taaaagctgc acggtgtgca gcaacacggc ttggcctgca
     5641 atttcggtgt attgtttcgg aatctggcta ccaaaacggc tgcccactcc ggcagccggt
     5701 accacagcaa aaaatctttg tgtcatgctt gtgactcgtg catttgttga cggtagaggc
     5761 tctcaccacc gacagccttg tccaaaacct tgacataagc ctcatgtgct gcggtttcgt
     5821 cttcatccat ggggatgact ttgaaactca tgtttacagg ccgcttgaat tggcccaatg
     5881 cttgcccgcc gtccttgcca tcgtcggcac ttttcacatc caaactgaat tggccacggg
     5941 tcatgcccaa atacacttcg gccaacaact cgcaatctat caatgcgcca tgcaacaccc
     6001 gcttggtttt gtccacaccc aagcggttac acaaagcatc caaattgact ttctgcccag
     6061 gataaagctt gcgcgccatg gccaccgtat cggtgacttt gctcaccaca gtgtctaatt
     6121 tgggcatgcc aagacgacca aattccatgt ccaaaaagcc cacgtcaaac gcggcattgt
     6181 ggatgattaa ctcactgtcg cgcaaaaagt ctgcaatctc agtggcaacg cctgcgaact
     6241 tgggtttgtc cgccaacatg tccaaggtaa tgccatgtac cgctatcgct tcttcggcga
     6301 tgccacgttc cgggtgcaca tacacatgca aatactcacc cgtgagtcgg cggtctatca
     6361 tttctaaacc tgcaaactca atcaggcggt cgccttgatt ggcatacagg ccggtggttt
     6421 cggtatcgag tacgatttgt cttactgcca tgatatgcct tacttcaccg ccgaagcggc
     6481 aacaggtgct gaggccgctt gctcctcact cgcagccgca gctccttcat ccatcaaatc
     6541 gtcaatgttt tgctgcggcg cggtttcaaa gcgtttgccg ttaagcaaca attcattgtt
     6601 tttcaccacc atctcactgt caacaatgcc attttgttgg gtcaaataac ctgcactggc
     6661 catgttattg atggtagagc gcaccaacat gcgtgcggtg tcgcgcactt cttccatgct
     6721 tggtgggttt tccacatcag ggtctactg
//
LOCUS       DAOMVK010000067         6716 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_3059_length_6716_cov_3.094280, whole genome shotgun sequence.
ACCESSION   DAOMVK010000067 DAOMVK010000000
VERSION     DAOMVK010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 6716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6716
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3059_length_6716_cov_3.094280"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..1463
                     /gene="gshA"
                     /locus_tag="PK856_03640"
     CDS             165..1463
                     /gene="gshA"
                     /locus_tag="PK856_03640"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HRL01500.1"
                     /translation="MLNQFEQQTAAFQQVILDKQTEIEAWFRAKWQQHTPPFYGSVDI
                     RNAGYKMSSIDMNLFPGGFNNINSDFLATAVIAAQDAVERACPTAKSILLVPENHTRN
                     TFYLQNVYALSKILEAAGFEVRVGSLNPEITAATTFTTALENELVLEPLLRVGDRLQL
                     EGGFNPCLVLLNNDLSAGVPEILQGLDQVVLPPLKGGWTTRRKTQHFAAYDKVAADFA
                     QVIGIDQWDINPYFKQASGINFHEREGEDQLADVVQRLLDKIQAKYDEKGITDTPFVI
                     VKADAGTYGMGVMSVKSADEVRKLNRKNRNKMAVVKEGLEVSEVIVQEGIYTYETVGE
                     AVSEPVVYMMDRFVIGGFYRVNEARGIDENLNATGMRFIPMDQEIPYQSEDADKRHYA
                     QWHSLGVCDKESPANCEDNRLYVYGVMARLSLLAASIELE"
     gene            1708..2718
                     /gene="trpS"
                     /locus_tag="PK856_03645"
     CDS             1708..2718
                     /gene="trpS"
                     /locus_tag="PK856_03645"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRL01501.1"
                     /translation="MTDKRVLTGVTTTGIPHLGNYVGAIRPAIAATQEAGVESFIFLA
                     DYHGLIKCHEPEKIHESTLAVAATWLACGLDPEHVTFYRQSDIPEIMELNWILNCICA
                     KGLMNRAHAYKAAVQANLDAGQEDVDNGVEMGLFCYPVLMAADILIFNGTHVPVGRDQ
                     IQHLEMARDIGARFNHRFKELFVAPEAVVDETTAILTGLDGRKMSKSYGNTIPLFEGE
                     KKLRKSVMKIVTNSLEPGQPKEVDGSTLFEIYQAFANKEQQAAMKQAYADGIGWGDAK
                     QQLFELINAELAEKRDHFAELTANPSRIEDILQAGATKARKVAHERLQQVREAVGIRP
                     LK"
     gene            3059..4006
                     /locus_tag="PK856_03650"
     CDS             3059..4006
                     /locus_tag="PK856_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01502.1"
                     /translation="MIAPEDLIRRFALGYWVVMLAIVLFIWLFVWLLIPKGHKWWVGI
                     LTSIAVMAVFIVPIAKVDIQEKQENTVKQEKYLVAKAVFDARCKNAGYKIYKTVEDVE
                     GVTLLNQWPDEKKYSDKMWEYAGLPDAYGGDSYIRDFLLWRKWDYKINDFFLDGQGFI
                     RNKPNIPPKADEINRYKYFNGFQYVDVLQNQSYQRYQFKNLYDLDETATTVIKKPSRY
                     TVEFENPIIPEERKIWIATTKAYVKDSKTGELLGEAAWHSFHGPQGEIKYSGTGVWDR
                     AKVCPYIADGQNNPIQYFVLKVLKPKQLSQEHKDDTLKN"
     gene            4278..4850
                     /locus_tag="PK856_03655"
     CDS             4278..4850
                     /locus_tag="PK856_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3'-deoxyribonucleotidase"
                     /protein_id="HRL01503.1"
                     /translation="MLILVDQDGVLANFEQAFYQAWVNSPMQAEFPALLPEARRHFYP
                     RDDYPAEFAQATRELIASKGFFRNLPPMPGAVAALSAMQAAGHEVVICTAPINEYRYC
                     VPEKYEWVEEHLGVEWTRRMVVTKDKTLVYGDVLIDDRPEIHGHRQPQWQHVLYDQPY
                     NRRMTHTPRLTWDNWQEVLSSLSPQPKHSE"
     gene            4857..5120
                     /locus_tag="PK856_03660"
     CDS             4857..5120
                     /locus_tag="PK856_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01504.1"
                     /translation="MTILLILVLLAAAILVLALAALSFTGNSAEKQLGYPKRGESMQD
                     VQFLLSIGENVLAQRCYRRIHRCSMSTAKLAVEKLAASQSAGN"
     gene            complement(5551..6057)
                     /locus_tag="PK856_03665"
     CDS             complement(5551..6057)
                     /locus_tag="PK856_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01505.1"
                     /translation="MPAPDKRLIISNQLEPLLVHLQPHIPLHLWISSSVLTEKTDVAN
                     VYAQLRQRHIPLHTVYAVGQGAQAANLEDCFNALWEAQQENMDVLADKDVLLSIACPY
                     EDEDFEFITSVMFLNAHFEDEADAHAGAGQYAAWFNPDIAFETHAIASLLNMAKQTQT
                     TILHVSGL"
     gene            6203..>6716
                     /locus_tag="PK856_03670"
     CDS             6203..>6716
                     /locus_tag="PK856_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HRL01506.1"
                     /translation="MELKQYVVDAFTQQLFAGNPAAVVVLDAFLETDLMQKIAAENNL
                     SETAFVVCQNGVYQIRWFSPLTEIDFCGHASLASAYVLQRFYAAGEHIVFACALLGEF
                     KINIAADGLISMVFPNRAPTEIATIPEALLTELSLAPQAVMQSPQAYFAIYANEQEVR
                     DLRIDEAPLKT"
BASE COUNT         1819 a         1460 c         1715 g         1722 t
ORIGIN      
        1 tggcttgtgc cgtgtgatga tagctttagg ccataacctt gaacagaggc acttgaccgc
       61 tgtgttacac ttattttttg gtctgttgat ttgtcataaa ctttaatgag acagatgcag
      121 tccaatattt caatttttca gaaagcctgc cgcttatgac tacgatgttg aatcagttcg
      181 aacagcaaac tgctgcgttt caacaagtga ttttagacaa acaaaccgaa atcgaggctt
      241 ggtttcgggc aaaatggcag cagcacacgc cgccttttta tggctcggta gacatccgca
      301 atgccggtta caaaatgtct tctatcgaca tgaatttgtt cccaggtggc ttcaacaaca
      361 tcaacagtga ttttttggcc acggccgtga ttgccgcaca agatgcggta gagcgtgctt
      421 gccctacggc caaatcgatt ttgttggtgc ctgaaaacca cacgcgcaac acgttttacc
      481 tgcaaaacgt ctatgcgttg agcaaaatct tggaagccgc gggctttgaa gtgcgcgtgg
      541 gctctttgaa tccagaaatc actgccgcca cgacgtttac caccgcattg gaaaacgaat
      601 tggtgttaga acccttgttg cgtgtgggcg accgcttaca attggaaggc ggttttaacc
      661 cttgcttggt cttgctcaac aacgatttgt ccgcaggtgt gcctgaaatt ttgcaaggct
      721 tagaccaagt ggtgttgcca ccactcaaag gcggctggac cacgcgccgc aaaacccaac
      781 actttgccgc ctatgacaaa gtggcagcgg actttgccca agtcatcggc atagaccaat
      841 gggacatcaa cccgtatttc aagcaagcca gcggcatcaa tttccacgag cgcgagggcg
      901 aagaccaatt ggccgatgtg gtgcagcgct tgctcgacaa aattcaggcc aaatacgatg
      961 aaaaaggcat taccgacacg ccgtttgtga ttgtgaaagc cgacgcgggc acttatggca
     1021 tgggtgtgat gagcgtgaag tctgccgacg aagtgcgcaa actcaaccgc aaaaaccgca
     1081 ataaaatggc ggtggtgaaa gagggtttgg aagtgtcgga agtcatcgtg caagaaggca
     1141 tttacactta cgaaaccgtg ggtgaagcgg tgtcagagcc tgtggtctac atgatggacc
     1201 gctttgtgat tggcgggttt taccgcgtga acgaggcgcg tggcatcgat gaaaacctca
     1261 atgccacagg catgcgtttc attccgatgg accaagaaat cccgtatcaa tctgaagacg
     1321 cagacaaacg ccattatgct cagtggcatt ctttgggcgt gtgcgataaa gaatcgcctg
     1381 ccaattgtga agacaatcgc ctgtatgttt acggcgtgat ggcacgtttg tctttattgg
     1441 cagcgtcaat tgaattagaa tagtcgcgaa tttgcaaaat ccacggacac agaccatgtt
     1501 gaaccacact ttccatcact ttgcgccaca cgccaatgcc tcattggtgg tgtctgtgcg
     1561 tggttggcgt gtgtggatga gccgttggca agctgcgtct gtgccatcca tgtaaactta
     1621 cgagcgattt tgtaccctta ataagcggcc tgtaagttgt tacaggccgc ttttatcatg
     1681 ttgttaaaca cagaaagaag aaacgtcatg actgacaagc gagtgttaac cggtgtgacc
     1741 accacaggca taccgcattt gggcaattac gtgggcgcca tccgcccagc catcgcggca
     1801 acccaagagg cgggtgttga atcgtttatt ttcttggccg attaccatgg tttgatcaaa
     1861 tgccatgagc ccgagaaaat tcacgaatca accctggctg tggctgccac ttggctggct
     1921 tgcggacttg acccagagca cgtcactttt taccgccaat ccgacattcc cgaaatcatg
     1981 gaactcaatt ggattttgaa ttgcatctgc gccaaaggct tgatgaaccg cgcccatgct
     2041 tacaaagccg cggtgcaagc gaatttggat gcgggccaag aagacgtgga caacggcgtg
     2101 gaaatgggct tgttttgcta tcccgtgttg atggcggccg acattttgat ttttaatggc
     2161 acccacgtgc ccgtaggccg tgaccaaatc caacacttgg aaatggcacg tgacatcggc
     2221 gcccgtttca accaccgttt caaagaattg tttgttgcac cagaagccgt ggtagatgaa
     2281 accaccgcga ttttaaccgg cttagatggt cgcaaaatga gcaaatcgta tggcaacacc
     2341 atccctttgt ttgaaggcga gaaaaaactg cgcaaatcgg tgatgaaaat cgtgaccaac
     2401 agtttggaac caggccagcc caaagaagta gacggcagca cgctgtttga aatctaccaa
     2461 gcgtttgcaa acaaagagca gcaagcagcg atgaaacaag cttacgccga tggcatcggc
     2521 tggggcgatg cgaaacagca attgtttgaa ttgattaacg ctgaattggc tgaaaaacgc
     2581 gaccacttcg ccgaactcac tgccaaccca agccgcatcg aagacatctt gcaagcaggc
     2641 gccaccaaag cccgaaaagt ggcgcatgag cgcttgcaac aagtgcgcga agccgtgggc
     2701 atccgtccat tgaaataagt ctaagcggga tgcaagcggc ctgtaaacct tgagtatggg
     2761 gtttacaggc cgcttttgat ttgtcagtgc gcaccgtatg gctaatgttg caaacagcca
     2821 agggcttaca gaccaaaggg taaatccaaa ttcaagcaaa ctgggagtga caaacaggag
     2881 ggtatgaact ggcagcgagt aaaaaatgag atggaatatg aatcaaccat tcttatggta
     2941 tcttaactca gtgttgaaga attaacggca ccggtgtttt acaagttttg tcgctgccat
     3001 caagaagcaa gtttggtaca caaaccatga aatttgcttt tccaacaagg gaaactcaat
     3061 gattgcacca gaagacttaa tcaggcggtt cgcgttaggg tattgggtgg tcatgttggc
     3121 catcgtactt tttatatggc tattcgtgtg gttgttgata cctaaggggc acaagtggtg
     3181 ggtgggtatt ttgaccagca ttgctgtgat ggcagtattc attgtgccaa tagcaaaggt
     3241 agacatccaa gagaaacaag agaatacggt taaacaagag aaatacttgg ttgccaaagc
     3301 ggtgtttgat gctcgctgca aaaatgcagg ctacaaaatt tacaaaacgg ttgaagacgt
     3361 tgaaggggtg acgttgttaa atcagtggcc tgatgagaaa aaatattctg acaaaatgtg
     3421 ggagtacgcg gggttacctg atgcatatgg tggggattca tacatacgag attttctctt
     3481 atggagaaaa tgggattata aaatcaatga ttttttctta gatggacagg gctttattcg
     3541 taataaacca aatattccgc caaaagcaga tgaaatcaat cggtataagt acttcaatgg
     3601 cttccaatat gttgatgttt tacaaaatca aagctatcaa cgctatcaat ttaaaaattt
     3661 atacgatttg gatgaaactg ccaccacagt cattaaaaag cccagtcgct atacggtgga
     3721 atttgaaaac cccattattc cagaagagcg caaaatttgg atagccacga ccaaagccta
     3781 cgtcaaagac agtaaaacag gagagttatt aggagaagcc gcttggcaca gttttcatgg
     3841 gccacagggt gaaattaaat actcaggaac gggtgtgtgg gatagggcaa aagtatgccc
     3901 atacattgct gacggtcaaa ataatcctat tcaatacttt gttttaaaag tgttaaaacc
     3961 aaagcaactt agtcaagagc ataaagatga cacactcaaa aattaatgac tttactgaat
     4021 attgggtggg cgattagcaa tgtttctttg tgacaatcac ttttgataca aataccgttg
     4081 cggcttttat gcataaaaca gcaagaacaa agggtagcaa cattgaggca tccgcatcgt
     4141 ttgtatattg ttggtgagca tgcatatact gacttagcca caatataagt ggcctgtaag
     4201 cttttaaaat cagcattaca ggccgcttgt gcatcagttg tattagaata cgctgtctaa
     4261 attatatggg tgcggttatg ttgatattgg tagaccaaga cggggtgttg gcgaattttg
     4321 aacaagcgtt ttatcaggct tgggtaaaca gccccatgca agcagagttt ccagctttat
     4381 tgcctgaggc gcgccgccat ttttacccgc gcgatgatta tccggcggaa tttgctcagg
     4441 ccacgcgtga gttgatagcg agcaaaggct tttttaggaa tttaccgccc atgccaggtg
     4501 cggtagcggc tttgagtgcg atgcaagcgg cggggcatga ggtggtgatt tgtacggcgc
     4561 ccattaatga ataccgatat tgcgtgcctg aaaaatacga atgggtggaa gaacacttgg
     4621 gagtggagtg gacgcggcgc atggtggtca ccaaagacaa gacgctggtg tatggtgatg
     4681 tgttgatcga tgacaggcct gaaattcacg gccaccgtca gccgcaatgg caacacgtgc
     4741 tgtacgacca accgtataac cgccgtatga cgcacacgcc gcgcttgact tgggacaatt
     4801 ggcaagaggt gttgtcaagc ttgtcgccgc agcctaaaca ctcagagtaa cagaccatga
     4861 cgattttatt gatactggtc ttgttagcag ctgcgatttt ggtactggcg ttggcggcat
     4921 tgtctttcac aggcaacagt gctgaaaagc aattgggcta tcccaagcgg ggcgaatcga
     4981 tgcaagacgt gcaatttttg ttgtcgatag gtgagaatgt gttggctcag cgctgctacc
     5041 gccgcatcca tcgttgttct atgagtactg ccaagcttgc agtggaaaaa ttagcagcaa
     5101 gccaatctgc tggcaattga taaaccttaa ctagatgcgg cctgtaaaca cgtgtccatc
     5161 tgcgctgagt gtatgtaggt ttatggtatg cacatgccat tgggttgcgt ataggaacac
     5221 tactcatgcc attttcgagc tagattcaag attgtggttg ctctgcttgg cctgatattg
     5281 gacacagaat ctagaaaatt tcatggtagt ggtgtattgg tgtctaaaat ctaaagatga
     5341 acgctgtttg ataaatatca aatgccacga atggatttag aacaataatc tctcgtcggc
     5401 ttcaccatcc catctggttg aaaaagactt caaagttcag cctcccatat tggcttataa
     5461 acttggggag cttggtccgc tactcagttc gactgtcttt caaaagccca ttgtgtacga
     5521 gcagtgggct tttgtttgcg tggcggtttg ttacaggccg ctgacatgca gtatcgtggt
     5581 ctgtgtctgc ttggccatat tcaataaaga tgcgatggca tgggtttcaa aggcgatgtc
     5641 tgggttaaac cacgctgcat attgtcctgc gccagcatgg gcgtcggctt catcttcaaa
     5701 atgagcattg agaaacatga cactggtgat gaattcaaaa tcctcatctt cataagggca
     5761 ggctatgctc agcagcacat ctttgtcggc taagacatcc atattctctt gttgtgcttc
     5821 ccacagcgca ttgaaacagt cttctaaatt ggcagcttgt gcgccttgcc caacggcata
     5881 aaccgtgtgc aaggggatgt gacgctgcct gagttgggca tacacattgg ccacatcggt
     5941 tttctcagtc aacacgctgc tggaaatcca taaatgcaaa ggaatatgcg gctgtaagtg
     6001 aaccagtagc ggctcaagtt gattgctgat gatgaggcgt ttgtctggag caggcatgtg
     6061 gcatcgcaat ggtcagtatg gacaagacca atacagctta acagcaaatc gattgtatgc
     6121 ggcctgtaag ccccgtaatc ttataggccg cttatgttat gctcattgct tgtttttaca
     6181 tctgtagaaa agagcggcga aaatggaatt aaagcaatat gtggtggatg cgtttactca
     6241 gcaattgttt gcaggcaatc ctgcggcagt ggtggtgttg gacgctttct tagagactga
     6301 tttgatgcaa aaaatcgcgg cggaaaacaa tttgtccgaa acagcatttg tggtgtgcca
     6361 aaacggtgtg taccaaatac gttggttttc accactgact gaaattgatt tttgcggcca
     6421 cgcgagcctt gccagtgcat atgtgttgca gcgcttttat gcggcgggtg agcacattgt
     6481 gtttgcttgt gctttgcttg gtgaatttaa aatcaacatc gctgccgatg gtttaatcag
     6541 catggtgttt cccaatcgcg cgcccacaga aattgcgacc atacctgagg ctttgttgac
     6601 agaattgtct ctcgcgccgc aagcggtgat gcaaagccca caagcctatt ttgccatcta
     6661 tgccaacgag caagaggtgc gtgatttgcg catagacgaa gcgccgctga aaacct
//
LOCUS       DAOMVK010000068         6536 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_3162_length_6536_cov_3.501929, whole genome shotgun sequence.
ACCESSION   DAOMVK010000068 DAOMVK010000000
VERSION     DAOMVK010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 6536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6536
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3162_length_6536_cov_3.501929"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="PK856_03675"
     CDS             <1..1062
                     /locus_tag="PK856_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRL01507.1"
                     /translation="CAGQCPVKVNVPDFKARFLQLYHNRYPRPWRDYAIAQLEYTLPV
                     AARFPKTYRRVMDAMWMKRLQERVLKLVDAPVLHEHSVVDWAALGATLLRPEQLSRLD
                     AQRYHAGNSVLLVQDAFTRYFDTAVWLDWIRLLQTLGIHVLVLPFHINGKALQVHGFL
                     GQFQRLVARNTAFLEQAAATGITMVGLDPAMTLVYRQEYPKALKLRQLPFDVCLPQEY
                     LATQAERLQAASAHSDVEYVLAGHCTEKTNAPESSQQWQNIFAWAGLTLRTVQTGCCG
                     MSGTYGHEAEHQVLSAKIYAQSWQPMLEQQATRQQVLATGYSCRSQVKRYSQLPLQHP
                     LQALLQHLNRHPNDNRRTP"
     gene            1059..2465
                     /locus_tag="PK856_03680"
     CDS             1059..2465
                     /locus_tag="PK856_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRL01508.1"
                     /translation="MSVSTIAPAQHTISPDLIRARFSQAMSDMYQQEVPLYGDLINLV
                     AEVNADFLEKHPDEAAVLAHSQELPRLNLERHGAIRVGTAEELATIRRLFAVMGMHPV
                     AYYDLAPAGVPVHSTAFRAITAEAMGVSPFRVFCSLLRLELIENIELRERAAAILAKR
                     CIFTDEALRLIALSEEQGGLNDADANRFVEAALETFRWHHEATVSAAEYQALADQHRL
                     IADVVAFKGPHINHLTPRTLDIDTVQRLMPDRGIDAKLSVEGPPQRQCALLLRQTSFK
                     ALNEDIRFTDQQAGEAGKHTARFGEIEQRGVALTAKGRALYDQLLDEAREKIGGAPTP
                     ANVDTYYQELARIFSVFPDAHADMHAQGLAFYHYRATEQGKAAAGSVAADASVETLLV
                     QGWLAIEPITYEDFLPVSAAGIFQSNLGGVERGGYASMSNQQLFERDLGQAVLSEIAL
                     YEQDSQASLQAALQQLGR"
     gene            complement(2750..3688)
                     /locus_tag="PK856_03685"
     CDS             complement(2750..3688)
                     /locus_tag="PK856_03685"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRL01509.1"
                     /translation="MKSHLALVFIGLGIVWGCNPYFTQLALPYLSPLEVVWWRVLCGF
                     IPLLLAALLTRSLSWQHWRYSGHFFMMSLLATSLYYWLYAHGAKLLPSGIAGVLSAAI
                     PMFTFAASSVLLRQQESITRFALIGMVVASLGIVCIAQPWQQHGAINGTGVMAIMAGS
                     AILGLSFVYARKFLAPLHIKPLALSTYQMGFAWVSLSLVTPLQNLGAVFQAPSASVAL
                     ILGLGVMGTGVAYLAYYYLIEKMGAILASTSTYVPPAVALIVGFVFASESINAIETVG
                     IVIMLLGVICIQFGAMWWHKWQGTASKHAAISKEKC"
     gene            complement(3822..4997)
                     /locus_tag="PK856_03690"
     CDS             complement(3822..4997)
                     /locus_tag="PK856_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HRL01510.1"
                     /translation="MHIIKSLMYFGSLSTMALALAACNQGNNSQAGSSSEAASNGDGI
                     TVTIGTVNPLTGPLTHWGVDSANGIKMAIEDANAKKMVWDGKPVTFKLISEDDIADPR
                     IATQMADRLVDAKVSAVVGHFTTSTAMPASLIYNTAGIPHITYSVTGAKFTQQGLDNV
                     YRVIANDSQQAKGMAKFAVDKLGMKTFVIAHDKGAYGEGLATDFAKEAEKLGAKMLST
                     EYTTATSTEFGSMATAIKTKQPDFVLYGGMDAQAGPLAKEMRRQGVKAKFMGSDGIKS
                     DELIKLMGPIDDVYASTAGSTTDKMHGYKDFSDRYQKEFKQPIVSFAPYAYDATNVII
                     TAMEQAKSSDPAKYKSFIKQTNLQGVTGPIAFDDKGDLKESAQTLYIVKDGKWEVVQ"
     gene            5360..5659
                     /locus_tag="PK856_03695"
     CDS             5360..5659
                     /locus_tag="PK856_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01511.1"
                     /translation="MMSVRTPYEGLQKTAAVPLVGGVVLFVLALLPVPVAWLLHGVFG
                     WWDAAALTRAVALVAALWNLGLSLFARTTISLFAIPCWLLFLLIGSLRYCGVMGA"
     gene            complement(5715..6356)
                     /gene="bioD"
                     /locus_tag="PK856_03700"
     CDS             complement(5715..6356)
                     /gene="bioD"
                     /locus_tag="PK856_03700"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009119910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HRL01512.1"
                     /translation="MIPGTFFISGIDTDIGKTMATGHIAKTAAAQGLRVITHKPIQTG
                     NVDVSEDIAKHRAIMGFGLLPVDEQKLTMPQLFPYPCSPHLASRLAERAIDFAAIESS
                     IEQLQQQFDTVLIEGAGGLMVPMTEDLLFIDYIKAKGYPVILVTSGRLGSINHTLLSL
                     EALHSRDIPLYALAYNQFHNSQDATINQDTYQYLCKHLAQYFPSAHIIDIPVL"
     gene            complement(6353..>6536)
                     /locus_tag="PK856_03705"
     CDS             complement(6353..>6536)
                     /locus_tag="PK856_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01513.1"
                     /translation="PMAVLQHMRQTGVSGISKQIWTKSKLQAFCHDYQATYATADGGV
                     CLSQHPLYLIARKKVS"
BASE COUNT         1497 a         1562 c         1843 g         1634 t
ORIGIN      
        1 tgcgcgggcc aatgccctgt caaagtgaac gtgcctgatt tcaaagcgcg ctttttgcag
       61 ctgtatcaca accgttatcc cagaccgtgg cgcgattatg cgattgccca attggaatac
      121 actttgcctg tggcagcgcg ctttcccaaa acctaccggc gcgtgatgga tgcgatgtgg
      181 atgaagcgtt tgcaagagcg ggtgttgaag ctggtggatg cgcctgtgtt gcatgagcac
      241 agtgtggtgg attgggcggc tttgggcgct accttgctcc gccctgaaca attgtcgcgc
      301 ttagacgccc agcgttatca cgcgggcaac agtgtgttgt tggtgcaaga cgcgttcacg
      361 cgctattttg atacggctgt gtggctggat tggatacggc tgttgcagac cttgggcata
      421 cacgtgttgg tgttgccgtt tcacatcaat ggcaaggcct tgcaagtgca tggtttcttg
      481 ggccagtttc agcgcttggt ggcgcgcaat accgcgtttt tagaacaagc cgccgccaca
      541 ggcatcacta tggtgggctt agaccccgcc atgactttgg tgtatcggca agaataccca
      601 aaagccttga agctgcgcca attgccgttt gatgtgtgtt tgccgcaaga gtatttggcc
      661 acccaagccg agcgtttaca ggccgcttct gctcacagcg atgtggagta tgtgttggca
      721 gggcattgca ccgaaaaaac caatgccccc gaatcgagcc agcaatggca aaacattttt
      781 gcgtgggcag gattgacctt gcgcacggtg caaacaggtt gctgtggcat gtcgggcacg
      841 tatggccacg aggcagagca ccaagtcttg tcggcaaaaa tctatgccca atcgtggcaa
      901 ccgatgctag agcaacaagc cacccgccaa caggtattgg ccacaggtta ttcctgccgc
      961 tcacaagtca aacgctacag tcagttgcca ttacaacatc ccttacaagc tttattacaa
     1021 catctaaatc gtcaccctaa tgacaacagg agaacaccat gagcgtcagc accatcgccc
     1081 cagcacagca caccatcagc cccgatttga tacgcgcgcg tttttcacaa gcgatgtctg
     1141 acatgtacca acaagaagtg ccgttgtatg gtgatttaat caatctggtg gccgaggtca
     1201 atgcggattt cttggaaaaa caccctgatg aagccgcggt tttggcgcac agccaagaat
     1261 tgccgcgctt gaatttggag cgccatggcg caatacgcgt gggcacggca gaagaattgg
     1321 ccaccatacg ccgcttgttt gcggtgatgg gcatgcatcc tgtggcgtat tacgatttgg
     1381 cgcctgcagg tgtgcctgtg cattccactg cgtttcgagc catcacggcg gaagcaatgg
     1441 gcgtgagccc gtttcgggtg ttttgctctt tgctgcgttt ggaattgata gaaaacatcg
     1501 aattgcgcga acgtgctgcg gcaattttgg ccaagcgctg cattttcacc gacgaggcct
     1561 tgcgcttgat tgccttgagc gaagagcaag gcggcctgaa cgatgccgat gcaaaccgct
     1621 ttgtcgaagc tgccttggaa accttccgct ggcaccatga agccacggtg agcgccgccg
     1681 aataccaagc cttggctgac cagcatcgct tgattgccga tgtggtggca ttcaaaggcc
     1741 cacacatcaa ccatttgacc ccgcgcactt tggacataga cacggtgcaa cgcctgatgc
     1801 ctgaccgtgg cattgatgcc aaattgtcgg tagaagggcc gccacagcgc caatgcgcgc
     1861 tgttgttgcg ccaaaccagt ttcaaagcct tgaatgaaga catccgcttt accgaccaac
     1921 aagcgggcga agcgggcaag cacaccgcgc gttttggtga gatagagcaa cgtggtgtgg
     1981 ctttaaccgc caaaggccgt gccttatacg accaattgtt ggatgaggcg cgtgaaaaaa
     2041 ttggcggtgc gcccacgcct gccaatgtgg acacgtatta ccaagagttg gcgcgcattt
     2101 tcagcgtgtt cccagatgcc catgccgaca tgcatgcgca aggcttggct ttctaccatt
     2161 accgtgcgac tgaacaaggc aaggcggctg cgggcagtgt ggcggcagat gcctcagtgg
     2221 aaaccttgtt ggtgcaaggc tggttggcga tagagccgat tacttacgaa gacttcttgc
     2281 ctgtgagcgc ggcaggcatc ttccaatcca atttgggcgg tgtggagcgc ggtggctatg
     2341 cttctatgtc gaatcagcaa ttgtttgagc gtgatttggg acaggctgtg ttgagcgaaa
     2401 ttgcgctgta cgagcaagac agccaagctt ctttacaggc cgcattgcag caattggggc
     2461 gataaacagc gtttacttgg gtagttcttt tgggtcttga ccaagacttg caccatcgcg
     2521 caatctgtgg cgatggtgtt tttttgtgcg ggtttggtgc gtgactgaag cgcaagaaag
     2581 gtgttaatga acgaatgaat ccttgcttgt gtgcatgact gcaattgacc ttaaagcggc
     2641 ctgtaaatcc ttacaagccg cttatgaata ccaatatggc tcaaatgctt tacccaaacc
     2701 atcaaacagt gttacaggcc gcttcaatat gccaacacag gcacaggcat caacattttt
     2761 ctttagaaat cgccgcgtgt ttgctggctg taccttgcca tttgtgccac cacatcgccc
     2821 cgaattggat gcaaatcaca cctaacaaca tgatgacaat gcccacggtt tcgatggcgt
     2881 tgatgctttc gctcgcaaac acaaagccca caatcaaggc caccgcaggc ggcacatagg
     2941 tggaggtaga cgcgagtatc gcccccattt tttcaatcaa atagtaatac gccaaatagg
     3001 ccacgcccgt tcccatcacg cccaagccca agattaaggc cacgctggca ctgggtgctt
     3061 gaaacactgc gcccaagttt tgcaaaggtg tcaccaagct gaggctgacc caagcaaagc
     3121 ccatttgata agtcgacaaa gccaggggct tgatgtgcag cggcgccaaa aatttgcgcg
     3181 cgtacacaaa cgacaaaccc aaaatggctg atccagccat gatcgccatc acgcctgtgc
     3241 cgttgatggc accgtgttgc tgccacggct gcgcgatgca cacaatgccc aagctcgcca
     3301 ccaccatgcc gatgagcgca aaacgggtga tggattcttg ttggcgtaac aacaccgaac
     3361 tggcagcaaa agtgaacatc ggtatcgccg cgctcaacac gcctgcaatg cccgatggca
     3421 agagtttggc accgtgggca tacagccagt aatacaagga agtggccagc agcgacatca
     3481 tgaaaaaatg cccagagtag cgccaatgtt gccagcttaa agagcgggtc agcaaagctg
     3541 ccagcaataa gggaatgaag ccacacaata cccgccacca cacgacttct aaagggctta
     3601 aatacggcaa ggccaattga gtgaaatagg gattgcatcc ccaaacaatg cccaagccaa
     3661 tgaataccaa agcgaggtgg cttttcatag tgttccaagt aaagtcggtg tgttagcgct
     3721 tgcgacagcg gcgcaagcaa ggtggtgtgt tcaagcgttt acagcgcaaa acaccttatt
     3781 cacaaggaat aaggtgttgg tttggatggg agtgagttgg cttattgcac cacttcccat
     3841 ttaccgtctt tcacaatgta cagggtttgg gcactttctt tcaagtcacc tttgtcatca
     3901 aaggcaatcg gacctgtcac gccttgtaaa ttggtttgct tgatgaagga tttgtatttg
     3961 gcagggtcgc tgctcttggc ttgttccatc gctgtgatga tgacattggt agcgtcatac
     4021 gcataaggtg caaacgacac aatcggttgc ttgaattctt tttggtagcg gtctgagaag
     4081 tctttatagc catgcatttt gtcggtggta gagcctgcgg tagaggcgta tacatcgtca
     4141 atagggccca tcaatttgat taactcatcg ctcttgatgc cgtctgagcc catgaatttg
     4201 gctttcacgc cttggcggcg catttctttg gccaaaggcc ctgcttgggc atccatgccg
     4261 ccatacaaga caaaatctgg ttgtttggtt ttgatggcgg tggccataga gccaaactcg
     4321 gtggaagtgg cggtggtgta ttcggtagag agcattttcg cacccaattt ttcggcttct
     4381 ttggcaaagt ctgtggccaa gccttcgccg tatgcgcctt tgtcatgtgc aatgacaaaa
     4441 gtcttcatgc ccagtttgtc cacggcgaat ttggccatgc ctttggcttg ttgtgaatcg
     4501 ttggcgatga cgcggtaaac attgtccaaa ccttgttggg tgaatttggc gcctgttacc
     4561 gaataagtga tgtgaggaat gcctgcggtg ttgtaaatca agctcgctgg catggcggta
     4621 ctggtggtga agtggcccac caccgcagac actttggcat caaccaagcg gtcggccatt
     4681 tgggtggcaa tgcgcggatc ggcgatgtcg tcttcggaaa tcagtttgaa ggtcacaggt
     4741 ttgccatccc acaccatttt tttggcattg gcgtcttcaa tcgccatttt gatgccattg
     4801 gcagaatcca caccccagtg ggtgagtggg ccagtcaatg gattgaccgt gcctatggtc
     4861 acagtaatgc catcgccatt gcttgcggct tctgagcttg agcctgcttg gctattattg
     4921 ccttggttac aggccgctaa agccaaagcc atggtgctga ggctgccaaa atacatgagg
     4981 gatttgatga tgtgcatagc cgatttcctt tgtgatttgt gggttttgtt tttcttgcag
     5041 gctaattaac aatagggatg aggacaaacg aaaaaactga aactattcat tccgattcag
     5101 aatactgaac tgtgaacatc ttgtttgaat aaggttgaaa tcatgggtat tcaatgtctg
     5161 tatggcagtt aggagtaggt aagatattat tgatgagtag aggtttacag gccgcttgta
     5221 cgcgatagcg aagatatggc ttaaatatgc actgtcgaat gaatgtggtg tcgggcattt
     5281 tattgctcaa atgggtgaca ggtgatgcaa aataacggcg agacttaaag tgaaacaagc
     5341 agattgaaag gatgccaaca tgatgtcggt gcgcacacca tatgaaggtt tgcaaaagac
     5401 ggctgccgtg cctttggtgg gtggagtggt gttgtttgtg ttggctttgt tgcccgtgcc
     5461 tgtggcgtgg ttgctgcatg gggtgtttgg ttggtgggat gcggcggcgt tgacgcgggc
     5521 ggtggctttg gtggcagcac tgtggaatct tggcttgagc ctgtttgccc gcaccaccat
     5581 cagcctgttt gcgataccgt gttggctgct ttttttattg ataggcagtt tgcgttattg
     5641 cggggttatg ggtgcctgaa gtgggctgtt tgcatgccca agcggcctgt aaatctgtta
     5701 caggccgctt gggtttacaa cacaggaatg tcgatgatgt gcgcagatgg aaagtattgc
     5761 gccaaatgct tgcacagata ttggtaagtg tcttggttga tggtggcgtc ttggctgttg
     5821 tgaaactgat tgtaggccaa ggcatagagg ggaatgtcgc ggctgtgcaa ggcttcgagg
     5881 ctgagcaaag tgtggttgat gctgcccaag cggccgctgg tcaccaagat tacgggatag
     5941 cctttggcct tgatgtagtc gatgaagagc aagtcttccg tcattggtac cattaagccg
     6001 cctgcgcctt ctatcaatac ggtgtcaaac tgttgctgca attgctcaat ggaactttca
     6061 atcgcggcaa agtcaatcgc acgctcggcc aagcgactgg ccaaatgggg cgagcaaggg
     6121 tagggaaaca attgcggcat ggtcagcttt tgttcgtcca caggcaagag gccgaagccc
     6181 atgatggcgc ggtgtttggc gatgtcttcg gacacgtcca cattgcctgt ttggatgggc
     6241 ttgtgggtaa tcacgcgcaa gccttgggcg gcggcggttt tggcgatgtg gcctgtggcc
     6301 atggttttgc cgatgtcggt gtctatgccg ctgatgaaaa atgtgccggg tatcatgaaa
     6361 ccttctttct ggcgatgaga taaagcgggt gttggcttaa gcacacgccg ccgtctgcgg
     6421 tggcgtaggt ggcttggtaa tcgtggcaaa aggcttgtaa tttggatttg gtccatatct
     6481 gtttgctgat gccagacacc cctgtttggc gcatgtgttg caacacggcc atgggg
//
LOCUS       DAOMVK010000069         6527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_3169_length_6527_cov_3.217089, whole genome shotgun sequence.
ACCESSION   DAOMVK010000069 DAOMVK010000000
VERSION     DAOMVK010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6527
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3169_length_6527_cov_3.217089"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..442)
                     /locus_tag="PK856_03710"
     CDS             complement(161..442)
                     /locus_tag="PK856_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HRL01514.1"
                     /translation="MEQLEFFDIPSPCVGVCISNNRGYCKGCFRSRDERLYWLKLSDA
                     QKREVVRLCAQRKRKVWLAKMQNLSVDVEQEEHLDEPELNLDFKDEQAE"
     gene            complement(584..4414)
                     /locus_tag="PK856_03715"
     CDS             complement(584..4414)
                     /locus_tag="PK856_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HRL01515.1"
                     /translation="MTANARIREIPYNYTSYSDKEIIIRLIGQSAWDILEHLRSQRRT
                     GRSAIMLFEVLGDIWAVVRNPYLVDDLLEHKHRQEALVREMRHRLNEIQKRRDDNEEV
                     AELLRLATQAVDDFDASFEKTRVKREQIVQRLSRITKKHNIMFDGLARVSHVTDATDW
                     RVEYPFVVINPDSEAEIAPLVKSLIELDLTIIPRGGGTGYTGGAIPLDAMSAVINTEK
                     LDLHMGVQMTDLPGLEGKHPVIHCGAGVVTRRVEEAATAAGFVFAVDPTSADASCVGG
                     NVAMNAGGKKAVLWGTALDNLAYWKMVNPQGQWLLIERVNHNMGKIHDQEIAEFDVHT
                     LKDDGKTIVSTERLSIPGHKFRKVGLGKDVTDKFLSGVPGVQKEGTDGIITSVAFVLH
                     TMPKHTRTVCLEFFGTVANATPAIVEIRDYLLGHDSVKLAGLEHLDWRYVRAVGYATK
                     AAGKGRPKMVLLADLVSDDENAVQEAAAHIVRFANARDGEGFIAVSPEARKTFWLDRS
                     RTAAIAKHTNAFKINEDVVIPLERLGEYSDSIERINIELSIQNKLALVRALEAYLRNE
                     KLPVDKMGEDLPSKELLGERGGYALKHVEAIGERWQWLLDNLDAPLSEYVERFGEGVL
                     EDNSADLSNTCFIAMRDFKLRVSIKRDLMRHLGEIFSGHVDKKIMEQLGKIHGKVVRS
                     RVFVALHMHAGDGNVHTNIPVNSDDYEMLQVAHKAVDRIMKIARDLNGVISGEHGIGI
                     TKLEYLTDEEMQPFWDYKAQVDPTGRFNRGKLMRGADLRMAYTPSFELLGVESLIMEQ
                     SDIGKIAHSVKDCLRCGKCKPVCTTHVPRANLLYSPRNKILGVGLLTEAFLYEEQTRR
                     GVSLKHFEEFNNVADHCTVCHRCEKPCPVNIDFGDVTVDMRDFLRKQGKKKFNPTAAI
                     GMAFLNAKDPATVKALRVGVVNMGFKAQNAAYDMAKKFSFGKLKKQKKQPDATTQQPT
                     IQEQVVHFINRPLPRNVPIKTARALLNIEDNKTVPIIRNPQLHEDTEAVFYFPGCGSE
                     RLFSQVGLATQAMLWHVGVQTVLPPGYLCCGYPQKGAGNVEKAQEITTDNRVLFHRMS
                     NTLNYLDIKTVVVSCGTCYDQLQEYRFEEIFPGCRIIDIHEFLLEKGVKLEGVKGQQY
                     LYHDPCHTPIKTMQPAKLASELMGQNVPLSDRCCGEAGTFAVNRPDIATQVRFRKQEE
                     IEKGKAALPQGEPVKILTSCPACLQGLSRYKDDANVDADYIVIEMAKHVLGEQWQNEF
                     VAKAANGGIERVLL"
     gene            complement(4987..5062)
                     /locus_tag="PK856_03720"
     tRNA            complement(4987..5062)
                     /locus_tag="PK856_03720"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5027..5029),aa:Ala,seq:ggc)
     gene            complement(5370..5768)
                     /gene="msrB"
                     /locus_tag="PK856_03725"
     CDS             complement(5370..5768)
                     /gene="msrB"
                     /locus_tag="PK856_03725"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HRL01516.1"
                     /translation="MEKIHKTNAEWRELLPDDVYYITREAGTERPYTGRYNDFHETGD
                     YHCACCDALFFTHQSKFDSHCGWPAFFAEAEPEATYRIVDQSHGMVREEVRCSTCDAH
                     LGHVFPDGPPPTGERYCINSLSMTFVNAED"
     gene            5884..>6527
                     /gene="mltG"
                     /locus_tag="PK856_03730"
     CDS             5884..>6527
                     /gene="mltG"
                     /locus_tag="PK856_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRL01517.1"
                     /translation="MAKKWMVVLAAVLATMVAIFALLLFLPKSNSGTERILVKKGDTI
                     SSLSRQLDEKSLIYSRPFLVLSSKILGRSQVRPGQYRLPQSISTWDILQKLAGNPDSL
                     FVRIPEGSTFADMRAIINQLPHLEHETKGLSTQALLKKIDPEQGAQHPEGLFFPDTYE
                     YTPGMSDVELYKTSYTKMQNELQDAWGDRQSNLPYKTPYELLIMASIIEKETAHP"
BASE COUNT         1696 a         1739 c         1421 g         1671 t
ORIGIN      
        1 ctgctcatgg caatattggg tcgcaacaaa atcaaggttg cggcaatcgc caccacataa
       61 ttcactgcca ccgcttgagc ggcctgtaaa ccccgtggcc gcgcccattt gaacaacact
      121 gacaccatca cactgcacaa cacacacaac accaaagcca tcattctgct tgttcatcct
      181 taaaatctaa attcaattct ggctcatcca aatgctcttc ttgctcgaca tcaacactga
      241 ggttttgcat tttcgctagc cacactttgc gcttgcgctg cgcacacaag cgcaccactt
      301 cgcgcttttg cgcatcactg agtttgagcc aatacaaacg ctcatcgcgt gaacgaaaac
      361 agcctttgca atagccgcga ttgttggaaa tgcacacgcc cacgcatggg ctgggaatat
      421 cgaaaaattc cagttgttcc atcacggtgc acctcaaaat cagcggcctg aaaacggcca
      481 ttgtagccac attccgacaa taccgcagct accacacttc ttgccgccat aaaaaacgct
      541 gctgtggcat gccacaacag cgctgtactg gtttacaggc cgcttagagc aagacgcgct
      601 ctatgccacc attggcggct ttggccacaa actcgttttg ccattgctcg cccaacacat
      661 gcttggccat ctcaatcacg atgtagtcgg catcgacatt ggcatcgtct ttgtagcggc
      721 tcaagccttg caaacacgca gggcaagaag tgaggatttt cactggctcg ccttgcggca
      781 aggcggcttt gcctttttcg atttcctctt gtttgcggaa acgcacttgc gtggcaatgt
      841 caggacggtt caccgcaaat gtgcccgctt caccacaaca acggtctgac aatggcacgt
      901 tttgacccat caattccgac gccaatttcg ctggctgcat ggtcttgatt ggggtgtgac
      961 atgggtcgtg gtacaaatac tgctgacctt tcaccccttc caatttcacg cctttttcga
     1021 gcaagaattc atggatgtcg atgatgcggc aacctgggaa aatttcctca aagcgatact
     1081 cttgcaattg gtcgtaacac gtaccacagc tcaccaccac ggttttgatg tccaaataat
     1141 tcaaagtatt ggacatgcgg tggaataaca cgcggttgtc ggtggtgatt tcctgtgctt
     1201 tttccacatt gcctgcgcct ttttgcggat aaccacagca caaatagcct ggaggcaaca
     1261 cggtttgcac acccacatgc cagagcatgg cttgcgtggc caaacccact tgactgaaca
     1321 aacgctcaga gccacaacca gggaagtaga acaccgcttc cgtgtcttcg tgcaattgtg
     1381 gattgcgaat gatcggcacg gttttgttgt cttcaatgtt aagtaaggca cgcgcggttt
     1441 taatcggcac attgcgtggc aagggtcgat taatgaaatg caccacttgc tcttgtatcg
     1501 ttggctgctg cgtggtcgcg tctggctgtt tcttctgctt cttcaatttg ccgaagctga
     1561 atttcttggc catgtcataa gcggcgtttt gcgccttaaa gcccatgttc accacgccca
     1621 cacgcaaggc tttcaccgtg gcagggtctt tggcattcaa gaaagccatg ccgattgccg
     1681 ccgttgggtt gaatttcttt ttgccttgtt tgcgcaagaa gtcgcgcatg tccacagtga
     1741 catcaccaaa gtcgatgttc acaggacaag gcttttcaca acggtgacac acggtacagt
     1801 ggtcggccac attgttgaat tcttcaaaat gcttgagcga cacgccgcga cgggtttgtt
     1861 cttcgtacaa aaacgcttca gtgagcaaac ccacacccaa aatcttgttg cgtgggctgt
     1921 acaacaaatt cgcacgcggc acatgggtgg tacacacagg cttacactta ccacagcgca
     1981 aacagtcttt caccgaatga gcgatcttgc cgatgtcaga ttgctccatg atcaaggatt
     2041 ccacgccaag caattcaaac gatggtgtgt aagccatgcg caaatcggcg ccgcgcatca
     2101 atttaccgcg gttaaagcgg cctgtaggat cgacttgggc tttgtaatcc caaaacggct
     2161 gcatttcttc gtcggtcaaa tactccaact tggtgatgcc gatgccgtgt tcacccgaaa
     2221 tcacgccatt caaatcacgg gcgattttca tgatgcggtc tacggctttg tgggcgactt
     2281 gcaacatctc gtaatcatca gagttcacag ggatattggt gtggacattg ccatcgcccg
     2341 catgcatgtg caaggccacg aacacacgtg accgcaccac tttaccgtgt atcttaccca
     2401 attgctccat gattttcttg tcaacatggc cgctgaaaat ctcacccaaa tggcgcatca
     2461 aatcacgctt gatggacaca cgcaatttaa agtcccgcat agcaataaaa caggtattgc
     2521 tcaagtcggc gctattgtct tccagcacgc cttcaccaaa acgctccaca tattctgaca
     2581 aaggcgcatc caaattatcg agcagccact gccagcgttc gcctatggct tcaacgtgtt
     2641 tcaaggcata gccgccacgt tcacccaaca attctttgct cggcaaatcc tcgcccatct
     2701 tatcgacagg caatttttca ttgcgtaaat aggcttctaa ggcgcgcacc aaagccaatt
     2761 tgttttgtat cgacaattca atgttgatgc gctcgatgct gtctgaatac tcacccaaac
     2821 gctcaagcgg aatcaccacg tcttcattga ttttgaaggc attggtgtgt ttggcaatcg
     2881 ccgcggtgcg cgaacggtct agccagaagg ttttgcgcgc ttcaggcgac acagcaataa
     2941 aaccttcgcc atcacgggca ttggcaaaac gcactatgtg cgcagccgct tcttgcaccg
     3001 cattttcatc atctgataca aggtcagcca gcaacaccat tttcggacgg cctttaccgg
     3061 ccgctttggt ggcataaccc accgcacgca catagcgcca atccaaatgc tccaagccag
     3121 cgagtttcac actgtcatgg cccaacaaat aatcgcggat ttccacaatc gcaggcgtgg
     3181 cattcgcaac cgtaccaaaa aactccaaac acacggtgcg tgtgtgtttc ggcatggtgt
     3241 gcaacacaaa ggccacgctg gtgatgatgc cgtctgtgcc ctctttttgc acgccaggca
     3301 cgcctgacaa gaatttatct gtcacgtctt tgcccaaacc gactttgcgg aatttgtggc
     3361 caggtatgct caagcgttca gtagacacga tggtttttcc atcgtctttt aaggtgtgta
     3421 catcaaattc ggcgatttct tggtcgtgaa ttttgcccat attgtggttc acgcgctcaa
     3481 tcaacagcca ttggccttgc ggattgacca ttttccaata ggccaaatta tccaaggcag
     3541 tgccccacaa cacggctttt ttaccgcccg cattcatcgc cacgttaccg cccacacaag
     3601 acgcgtcggc tgaggtcggg tctactgcaa acacaaagcc tgccgctgtg gcggcttctt
     3661 ccacacggcg cgtcaccaca cctgcaccac aatggataac agggtgtttg ccttccaagc
     3721 ctggtaaatc cgtcatttgc acacccatgt gcaaatcgag tttttcggta ttgatcaccg
     3781 cactcatggc atccaacgga atcgcaccgc cggtataacc cgtaccacca ccacgcggaa
     3841 tgatggtcaa atccagctca atcaatgatt tcaccaaagg tgcgatttcg gcttcagaat
     3901 cgggattaat caccacgaat gggtattcca ctcgccagtc ggtggcatcg gtcacgtgcg
     3961 acacacgcgc caaaccatca aacatgatgt tgtgcttttt ggtgatgcgg ctcaaacgct
     4021 gcacgatttg ttcgcgtttg acacgggttt tctcaaaaga ggcatcaaaa tcatccacag
     4081 cttgggtggc caaacgcaat aattctgcaa cttcttcatt gtcgtcacgg cgcttttgga
     4141 tttcgttcag acggtgacgc atttcgcgca ccaaggcttc ttgacggtgt ttgtgctcca
     4201 acaagtcatc gaccaaataa ggattgcgca ccactgccca aatgtcgccc aaaacctcaa
     4261 acaacatgat ggcagagcgg cctgtacggc gttgcgatcg caagtgctct aagatgtccc
     4321 aagccgattg gccaatcagc cggatgatga tttctttatc tgaataagat gtgtagttgt
     4381 acggaatttc gcgaatgcgc gcgttagcag tcattggggt tccattaacg atttttgtta
     4441 tttgccgcca gaaaattgtt gcaaagcgac aattacacag tttaaccgat agaccccgtc
     4501 ctgaaaaccc aaaaccgaca gtattgtcat ctggctgcca tataaagcca atattttaaa
     4561 gaattcaaca aaatcatgtg tttttgacac atctgttatg tcgcttgccc gccgacattt
     4621 ggtgcaaata gtctgtgttt caacactcta aaatggctgc tttcaaacaa ggtgggccgc
     4681 catttttaac cgataaatgc gtttttatag cgtctacacc tactactcgc aaacaagcgg
     4741 cctgtaaaat tcacaggccg cttgtttgca gtcatttcaa aatacatgat ctgtacggta
     4801 tcaaccctac tcaccgacac catttgccat tttgatgttt atattaaatg gtgttagtac
     4861 cgcataattt gtcatacatt tcactaagtc agtgcatact cagcaaacca acttacaaag
     4921 gctaaaatgc ggccttaaca gatcaaatga aaaaagcctg acttgaaagt caggctttta
     4981 gaattttggt ggaggtaagc gggatcgaac cgctgacctc ttgcatgcca tgcaagcgct
     5041 ctaccaactg agctataccc ccaaagagat gtgcataata tcagcatttt ttaggtttgc
     5101 aaaccgtcca aattcaaaaa tcagtctgtt ttttgaattt taaaaataaa catttgatgt
     5161 atctgctcat ttttatgcat cttgcgctaa cgcaaaagga ctcatacctg aatcgatatg
     5221 aaaaatacaa caatggttgc agtttcgcgg cactttaaac aatgcctttg cattcactgc
     5281 aatactgaat agatgcttat tgagttaaac ctattaagcg gcctgtaaac atgaccatct
     5341 cgtttacagg ccgcttttat accatgcaat caatcttctg cattcacaaa cgtcatggac
     5401 aatgaattga tgcaatatcg ctcaccagtg ggcggcggcc catctggaaa tacatgcccc
     5461 aaatgggcat cacaagtgct gcaacggact tcttcacgca ccatgccatg gctttgatcc
     5521 acaatgcggt aagtggcttc aggctcagcc tcagcaaaaa aagcaggcca gccgcaatgg
     5581 ctgtcgaatt tgctctggtg ggtaaaaaac aaagcatcac aacaagcgca gtggtaatca
     5641 cctgtttcat gaaaatcatt gtaacggcct gtataagggc gttcagtccc agcctctcgc
     5701 gttatataat acacatcgtc cggcagcagc tctcgccact cggcattggt tttgtggatt
     5761 ttttccatgg tgatgatcct ttctatcgcc cgtctgcctt attattacac ccgtattgca
     5821 agccacttct tcagggcttg gcaatggttt tgaatacttc gtttaattac agaatgagtc
     5881 attatggcta aaaaatggat ggtggtgtta gcagcggtgc tggccaccat ggtagcaata
     5941 ttcgctttat tgttgttttt acccaaaagc aacagcggca ccgaacgcat tttggtgaaa
     6001 aaaggcgata cgatttctag cttgagccgt caattggatg aaaaaagcct gatttacagc
     6061 cgcccattct tggtgttgag cagcaaaatc ttggggcgta gccaagtcag acctggtcaa
     6121 taccgcttgc cgcaaagcat ttccacttgg gacattttac aaaaactggc gggcaaccct
     6181 gacagcttgt ttgtgcgcat tcctgaaggc agcacatttg ccgacatgcg cgccatcatc
     6241 aaccaattgc cccatttgga acacgaaacc aaaggccttt ccacccaggc cttgttaaaa
     6301 aaaatagacc cagaacaagg cgcacaacat cctgaaggct tgtttttccc agatacttat
     6361 gaatatacgc caggcatgag tgatgttgag ctgtacaaaa cctcgtatac caaaatgcaa
     6421 aatgaattac aggacgcatg gggagacaga caaagcaatt tgccatacaa aaccccatac
     6481 gaattgctga tcatggccag catcatcgaa aaagaaaccg ctcaccc
//
LOCUS       DAOMVK010000070         6517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_3173_length_6517_cov_3.376818, whole genome shotgun sequence.
ACCESSION   DAOMVK010000070 DAOMVK010000000
VERSION     DAOMVK010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 6517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6517
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3173_length_6517_cov_3.376818"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..2855
                     /locus_tag="PK856_03735"
     CDS             39..2855
                     /locus_tag="PK856_03735"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRL01518.1"
                     /translation="MEAAKYDPAQIESKWYQYWESNGFFEPQIDESKPSFAIQLPPPN
                     VTGTLHMGHAFNQTIMDGLTRYYRMRGYNTAWVPGTDHAGIATQIVVERQLSGQGINR
                     HDLGREAFLNKIWEWKEVSGGTITEQMRRVGCSVDWSREYFTMDDTRAEIVTDVFVRL
                     FEQGLIYRGKRLVNWDPVLGTAVSDLEVESVDETGSMWHIHYPLADDATQALIVATTR
                     PETLLGDVAVAVHPEDERYAAFIGKQVVLPLTGRTIPVIADEYVEKDFGTGCVKITPA
                     HDFNDYEVGKRHQTQLISVLDLTAKVLAEAEIYNFQGQAQGVIALPAAYAGLDRFDAR
                     KAIVADLETQGYMVKIDPHQLKTPRGDRTGSVIEPMLTSQWFVAMEKVAEGDETGLSL
                     AGKARQAVDSGEVKFVPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDDTGRCYV
                     AKTEAEAQALAGADVVLSRDEDVLDTWFSSALVPFSTLGWPEETPELKAFLPSSVLVT
                     GYEIIFFWVARMIMMTAHFTGKVPFKDVYIHGMVRDNEGKKMSKSEGNVIDPVDLIDG
                     IDLPHLLEKRTTGLRRPETAPKVIKATEKLFPEGIPVFGADALRFTMASYASLGRSIN
                     FDFKRAEGYRNFCNKLWNATRFVLMNVEGRDCGQDESLPLAYSFVDEWMLSRLQQTEA
                     AMADALNTYRFDIAAQTIYEFVWNEYCDWYVELAKVQLNSGDEASQRATRRVLVRVLE
                     VILRLLHPIMPFITEELWQTVAPLANAKHTDSIMVASWPVADASKIKQDSLDKMEVLQ
                     DLMGAVRNLRGEMNLGPAVKAPLFIEGGAELDICLRYLPMMARLSDVMRVDTLPEDDA
                     PVAVAYGARLMLKVEVDKAAETARLSKEQEKLQKALDKLTAKLEKPGYTDKAPAHLVE
                     KDRAELAQLNDKMAKIMEQLTKLK"
     gene            complement(2911..3195)
                     /locus_tag="PK856_03740"
     CDS             complement(2911..3195)
                     /locus_tag="PK856_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRL01519.1"
                     /translation="MKTFQNIDNIRDIRKKLGLNQQEFWSQIGVTQSGGSRYESGRNM
                     PKPVRELLRLVHVEQVDLAKVNHDDMVIAALLKVRHPDIYKTLKKEAKAK"
     gene            complement(3432..4208)
                     /gene="map"
                     /locus_tag="PK856_03745"
     CDS             complement(3432..4208)
                     /gene="map"
                     /locus_tag="PK856_03745"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRL01520.1"
                     /translation="MSVYINNDEDIAKMRASGTLAAELLDYIGDFVKPGVTTNELNQL
                     CHDYQLNVQGTIPAPLGYGEPPFPKSICTSVNHVICHGIPDDKALKKGDMLNIDVTII
                     KDGYHGDSSRMFMVGEVSPHAQRLSKVTHECMMLGIEQVKPGATLGDVGHAIQVHAEN
                     NGYSIVQEFCGHGVGKKFHCEPQVLHYGKRGTGLKLEPGMIFTIEPMLNQGKRHLRIL
                     ADGWTVVTKDRSLSAQWEHEVLVTETGFEILTVSPRTGQP"
     gene            complement(4258..5049)
                     /gene="trpC"
                     /locus_tag="PK856_03750"
     CDS             complement(4258..5049)
                     /gene="trpC"
                     /locus_tag="PK856_03750"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HRL01521.1"
                     /translation="MNDILKQILATKVEEVAAFRAQTSLEDIKAQAQDQEAARDFVGA
                     IRAKHAANLPAVIAEIKKASPSKGLIRPHDFNPANIAQIYEHAGAACLSVLTDEPYFQ
                     GAPEYLKQAKAACKLPVLRKDFMVDAYQIYQAKTWGADAILLIAAALEQTQLEEFEAI
                     AHELGMAVLLEVHHTDEWEKCQRLTTPLVGVNNRNLRTFAVDLQQTLDYLPVLDSNRI
                     IVTESGITTRAEVELMRKNGINTFLIGETFMRADDIGQAVTELLF"
     gene            complement(5046..6008)
                     /locus_tag="PK856_03755"
     CDS             complement(5046..6008)
                     /locus_tag="PK856_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HRL01522.1"
                     /translation="MQPYLAPAWLKGGGHVETILPKFIRSPALVFRRELIQDSTGQAH
                     VAYDFVDTPRPDTPLVVLFHGLEGNSNSHYAQALMNLVRQKQWLGVVVHYRGCGGVVN
                     LSDKAYHSGDSLECAWVLKLLTERHPNRRRYAMGVSLGGNVLAKYLGESGDEALLDAA
                     AVVSAPLDLVSASEALEHGLARTLYAPYFLRTLLPKVKQEQGFKATPELEWQKLFASR
                     SLKDFDNHYTAPVHGFSNADDYYQSSSAKPLLQHIQRPTLILNAQNDPFMPAKDLPTA
                     ADVSKQVCLYQPEQGGHVGFVSGSFPGHLNWLPQTVLTYFSEQT"
     gene            complement(5993..6265)
                     /locus_tag="PK856_03760"
     CDS             complement(5993..6265)
                     /locus_tag="PK856_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01523.1"
                     /translation="MHLNAACKPLQAASVHAKQHTHTPYARHSNAPHRQDEFTMLMPN
                     QETALLPMPSLCRTKLTLFMARPTMQFGTEQSVNCRLQAAYSCNHI"
     gene            complement(6265..>6517)
                     /gene="yjgA"
                     /locus_tag="PK856_03765"
     CDS             complement(6265..>6517)
                     /gene="yjgA"
                     /locus_tag="PK856_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HRL01524.1"
                     /translation="PIQAYLNKLKGDNAEHNAAMQRLELLRERLIESDDVLTELIADK
                     PELPISELRTLIRNARREKEQNKPPKAFRELFQLLKQHH"
BASE COUNT         1650 a         1486 c         1643 g         1738 t
ORIGIN      
        1 ttataatcat ggcttcgaat tttaaataaa gatgtgacat ggaagcagca aaatatgatc
       61 cagcgcaaat tgaaagcaag tggtatcaat attgggaaag caacggcttt tttgagcccc
      121 aaattgatga aagcaagcct tcttttgcca ttcaattgcc accacccaac gtaacaggca
      181 ctttgcacat gggccatgcg ttcaaccaaa ccatcatgga tggtttgacc cgctattacc
      241 gcatgcgagg ttacaacact gcttgggtgc caggtacaga ccatgcgggt attgccacac
      301 aaatcgtggt ggaacgtcaa ttgtcaggtc aaggtatcaa tcgccatgat ttgggtcgtg
      361 aagccttctt gaacaagatt tgggagtgga aagaggtttc aggtggcacc attacagagc
      421 aaatgcgccg tgtgggctgc tcagtcgatt ggtcacgcga gtatttcacc atggatgaca
      481 cacgcgctga aatcgtgacc gatgtgtttg tgcgcttgtt tgaacaaggt ttaatttatc
      541 gtggcaagcg cttggtaaat tgggaccctg tgttaggcac tgcggtttct gatttggaag
      601 tggagagcgt ggatgaaaca ggctctatgt ggcacatcca ttaccctctt gcagacgatg
      661 ccacccaagc tttaatcgtc gccacaaccc gtcctgaaac cttgttgggc gatgtggcag
      721 tggcggtgca tccagaagat gaacgctatg ctgctttcat tggcaaacaa gtggtgttgc
      781 cgcttacagg ccgcaccata cctgtaattg ccgatgagta tgtggaaaaa gactttggta
      841 caggctgtgt gaaaatcacg cctgcacatg attttaatga ctacgaagtg ggcaagcgcc
      901 atcaaaccca actcatcagt gtgttggatt tgactgccaa agtattggca gaggctgaaa
      961 tatacaattt ccaaggtcaa gcccaaggtg tgattgcctt gccggctgca tacgcaggct
     1021 tggatcgctt tgacgcccgc aaagcgatag tggccgattt ggaaacccaa ggctacatgg
     1081 tcaaaatcga tcctcatcaa ttgaaaaccc cgcgtggtga ccgcacgggt tcggtgattg
     1141 agcctatgtt gaccagccaa tggtttgtgg ccatggaaaa agtcgccgaa ggcgatgaaa
     1201 cgggtttgag tttggcaggt aaggcccgtc aagccgtgga cagtggcgaa gtgaagtttg
     1261 tgccagaaaa ctgggtaaac acctacaacc aatggatgaa caacattcaa gactggtgta
     1321 tctcacgcca attgtggtgg gggcatcaaa ttcctgcttg gtatgatgat acaggccgct
     1381 gctacgtggc caaaaccgaa gctgaagcgc aagccttggc tggtgcggat gtggtgttaa
     1441 gccgtgatga ggatgtgttg gacacttggt tcagctctgc tttggtgccg ttttctactt
     1501 tgggttggcc ggaagaaaca cctgagctca aagccttttt accaagctca gtgttggtca
     1561 ctggttatga aatcatcttc ttctgggtgg ctcgcatgat catgatgacc gctcacttta
     1621 ccggcaaagt accgtttaaa gacgtgtaca ttcatggcat ggtgcgtgat aacgaaggca
     1681 agaaaatgtc caaatctgag ggcaatgtca ttgacccagt ggatttgatc gatggcatcg
     1741 atttgccaca cttgttggag aagcgcacca ccggtttgcg ccgccctgaa accgcaccga
     1801 aagtgattaa ggccactgaa aagctgttcc ctgaaggcat acccgtgttt ggtgccgatg
     1861 ccttgcgttt caccatggcc agttatgcca gtttgggtcg ttcaatcaat ttcgatttca
     1921 aacgtgccga aggctaccgt aacttctgca ataagctgtg gaatgccacc cgttttgtgt
     1981 tgatgaatgt tgagggccgc gactgtggtc aagatgagag cttgccattg gcttattcgt
     2041 tcgttgacga atggatgttg tcacgcttac aacaaaccga agcggctatg gctgatgcac
     2101 tgaataccta ccgctttgat atcgcagcac aaaccattta cgagttcgtg tggaatgagt
     2161 attgcgattg gtatgtggaa ttggccaaag tgcagttgaa cagcggcgat gaggcttcac
     2221 aacgtgcgac acgccgagta ttggtgcgcg tattggaagt gattttgcgt ttgttgcacc
     2281 caattatgcc attcatcacc gaagagttgt ggcaaaccgt ggcacctttg gccaatgcca
     2341 aacacactga cagcatcatg gtcgcatctt ggcctgttgc ggacgcaagc aaaatcaaac
     2401 aagacagctt ggacaaaatg gaggtgttac aagacttgat gggcgcagtg cgcaatttgc
     2461 gtggtgagat gaatttgggt cccgcggtca aagcaccttt gttcattgaa ggtggtgccg
     2521 aattagacat ttgtctgcgc tatttgccga tgatggcgcg cttgagcgat gtgatgcgtg
     2581 tcgatacatt gccagaagac gatgcccctg tggctgtggc ttatggtgct cgcttgatgc
     2641 tgaaagtgga agttgacaag gctgctgaaa cggcccgttt gagcaaagag caagaaaaat
     2701 tgcaaaaagc attggacaag ctgactgcta aactggaaaa acctggttat accgacaaag
     2761 ctcctgcaca tttggtggaa aaagaccgtg cggagttggc gcagttgaat gacaagatgg
     2821 caaaaatcat ggaacaattg accaagctaa aataaatgct gaaatagaaa aagctgcctt
     2881 tgggcagctt ttttttggtg gagtaaacat ttacttggcc tttgcttctt ttttcagggt
     2941 tttgtaaatg tctggatggc gcacttttaa caaagcggca atcaccatgt catcatgatt
     3001 gaccttggcc aaatccactt gctcaacatg caccaaacgc agcaattcac gcactggttt
     3061 gggcatgttt cggcctgatt catagcgtga accacccgac tgagtcacac caatttggct
     3121 ccaaaactct tgttggttca gaccaagttt tttgcgtatg tcgcggatgt tgtcaatgtt
     3181 ttgaaaagtt ttcattgcga taccttagag tccttaagtt ttatcatggt tggtgtgtta
     3241 gtcacaccta ttattgccga tgacaaacgt aacaagatgt atgccatcag cttctttatg
     3301 agcataaatt atgccatgta gttttggaaa ctaaagtaaa gttttgtcac taaaagtgat
     3361 aaatcccaat atttaacaca atgaattatt tacagaaact ttgaaacacg aatttgatta
     3421 ataaatgaat actagggttg gccagttctg gggctgacgg tgaggatttc aaagccagtt
     3481 tccgtgacca agacctcgtg ttcccattgc gctgataaag agcggtcttt ggtcacaacg
     3541 gtccaaccat cggccaaaat gcgcaaatgg cgtttgcctt ggttcagcat cggttcaatg
     3601 gtgaaaatca taccaggttc caatttcaag cctgtcccac gtttgccata gtgcaaaacc
     3661 tgtggctcgc agtggaattt cttacccacg ccatgcccac agaattcttg cacgatggaa
     3721 tagccattgt tctcagcatg aacttgaatc gcatggccga catcacccaa agtagcgcca
     3781 ggtttcactt gctcaatgcc cagcatcatg cattcatggg tgactttaga caggcgttgt
     3841 gcatgtgggc tgacttcacc caccatgaac atgcggctgg agtcaccatg gtaaccgtct
     3901 ttgatgatgg tcacgtctat gttcaacatg tcgccttttt tcaaggcttt gtcatctggg
     3961 atcccatgac aaatcacatg gttgactgag gtgcaaatgg atttcgggaa cggtggttcg
     4021 ccatagccca aaggggcggg gatagtgcct tgtacgttta attgatagtc gtggcacaat
     4081 tgattcaatt cattggtggt cacgccaggt tttacaaagt cgccaatgta gtccaacaat
     4141 tcggcagcca aggtgcctga agcacgcatt ttggcgatgt cttcatcatt attgatgtat
     4201 acactcatga tgtttcctta tgtcatcggc ctgtaaagcg ttacaggccg cttatttcta
     4261 aaataataat tcggttactg cttgacctat gtcatctgct cgcatgaagg tttcaccgat
     4321 taagaaggta ttaatgccat ttttgcgcat taactccact tctgcgcgtg tggttatgcc
     4381 gctttctgtc acgatgatgc gatttgagtc cagcacaggc aggtaatcca aggtttgttg
     4441 taagtccact gcaaacgtgc gtaagttgcg gttgttcacc cccaccaacg gagtggtcaa
     4501 gcgttggcat ttttcccatt catccgtgtg atgcacttcc aataacacgg ccatgcctaa
     4561 ctcatgcgca atggcttcaa actcttccag ctgagtttgt tccaatgccg ctgcaatcaa
     4621 caaaatcgca tcggcccccc aggttttggc ttggtagatt tgataggcat ctaccatgaa
     4681 gtctttgcgc aatacaggca gcttacaggc cgctttggcc tgctttaaat attcgggcgc
     4741 accttgaaaa taaggctcat cggtcaacac cgataaacaa gccgctcccg cgtgctcata
     4801 aatctgagca atgttggcag gattgaagtc gtgtgggcgt atcaagcctt tggatgggct
     4861 ggctttcttg atttcagcaa tcaccgcagg caagttagcg gcatgtttgg cacgaatggc
     4921 accaacaaaa tcacgagccg cttcttggtc ttgtgcttgg gctttgatgt cttccaaaga
     4981 agtctgcgca cggaaagcgg ccacttcttc caccttggtg gccaatattt gttttaagat
     5041 gtcgttcatg tttgctcact gaaataagtt aataccgttt gcggtagcca atttaaatgc
     5101 ccaggaaagc tgccgctgac aaacccaaca tgcccacctt gctcgggctg gtacaaacac
     5161 acctgttttg acacgtcggc agccgttggc aaatccttgg caggcataaa aggatcgttt
     5221 tgcgcgttga gaatcaaagt cgggcgctgg atgtgttgca gtaaaggctt ggcactgctg
     5281 ctttggtagt aatcatcggc attgctaaag ccatgtactg gggcagtgta gtggttgtca
     5341 aaatctttta agctgcggct ggcaaacagt ttttgccact caagttctgg tgtggcctta
     5401 aacccttgct cttgcttcac tttgggcagc aaggtacgca gaaaataagg ggcatacaaa
     5461 gtacgcgcca gaccatgctc taaagcttcg ctggcactga ccaaatccaa tggagcagac
     5521 accacagcag ctgcatccaa caatgcctca tcaccgcttt cacccaaata cttggccaaa
     5581 acattgccgc ccaaagacac gcccatagcg tagcggcggc gattgggatg gcgctcggtc
     5641 aatagcttta acacccaagc acattctaag ctgtcccctg aatgatacgc tttatcactt
     5701 aggttcacca caccaccaca gccccgatag tgtaccacca cacccagcca ttgtttttgc
     5761 ctcaccaagt tcattaaggc ctgtgcgtaa tggctgttgc tgttaccttc tagtccgtga
     5821 aacaaaacca caagaggcgt gtctggccga ggcgtatcga caaagtcata ggccacatgg
     5881 gcttggcctg tagagtcttg gattaattcg cgacgaaaca caagcgcagg cgagcgtatg
     5941 aatttgggca atatggtttc tacgtgacca ccgcctttta gccaagctgg tgctagatat
     6001 ggttgcatga ataagcggcc tgtaaacggc aattcaccga ttgttcagta ccaaattgca
     6061 ttgtgggtct ggccatgaat aaagtcagtt ttgtacggca cagacttggc attggtaaca
     6121 atgcggtttc ctgatttggc atcaacatgg tgaattcatc ttggcgatgt ggtgcattag
     6181 agtgacgtgc ataaggtgta tgggtgtgtt gcttggcatg gacagaagcg gcctgtaagg
     6241 gtttacaggc cgcatttaaa tgcatcaatg gtgttgcttg agcaattgga acaactcacg
     6301 aaacgcctta ggtggtttgt tttgctcttt ttcacggcgg gcattgcgga ttaaggtacg
     6361 caattcgcta atgggcaact ctggtttgtc ggcgatgagt tcagttaaga catcatcaga
     6421 ttcaatcagg cgctcacgta acaactccaa acgctgcatc gccgcattgt gctcggcatt
     6481 gtcgcccttg agtttgttca aataggcttg aatcggc
//
LOCUS       DAOMVK010000071         6490 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_3191_length_6490_cov_2.988345, whole genome shotgun sequence.
ACCESSION   DAOMVK010000071 DAOMVK010000000
VERSION     DAOMVK010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 6490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6490
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3191_length_6490_cov_2.988345"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1525
                     /gene="priA"
                     /locus_tag="PK856_03770"
     CDS             <1..1525
                     /gene="priA"
                     /locus_tag="PK856_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRL01525.1"
                     /translation="ITGSGKTEVYFAAMAEVLARGQQVLMLLPEINLTPQLLARINSR
                     FFGYEPAVLHSQAAVGARSHDFVRAMKGEAKLIIGTRLSVLTPMPNLGLIVVDEEHDG
                     SFKQQNELRYHARDVALWRAKQAGCPIVLGSATPSLESWNNAITGRFALLSLPERANA
                     QAVLPKIHVIDVRQLPLEEGMCEPVKQALLKNWQQGGMSLVYLNRRGFAPVLACTACG
                     HTFDCPNCSAKMVWHQNQRQLRCHHCDLREPVPVFCPECHNSDLTPLGQGTQRVEEAL
                     KALMPHANIVRVDRDTTSGKQDWQQLYEQVQADAVDVLIGTQMLAKGHDFAKLNLVVI
                     LNADGALYSSDFRAPEHLFAQLLQVSGRAGRAEQKGKVLLQTQLPDHPLFAALQQHDY
                     IGFAEQELASRQLYEFPPFSHMLAVRVDAPQMLEAQQLLQQVLDELELPDDVSVFGPV
                     PMLMARLNGRERAQVFVQSPSRQSLHKAGRLFQAALSAILPHQSAWRYALDMDVQDA"
     gene            1538..2923
                     /gene="dacB"
                     /locus_tag="PK856_03775"
     CDS             1538..2923
                     /gene="dacB"
                     /locus_tag="PK856_03775"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HRL01526.1"
                     /translation="MGNSLKKKHLHGAWLASALLWGSVHSAWALDLSGLKANEVAVYV
                     QNMDTGRTVLEHRADAAMNPASTMKLLTAWYSLNRLSPEYRIRTYWHSKAKIENGVLQ
                     GDLVWRGSGDPTLDHNALQEMMQQLQAAGVKHIQGQLVLDKSLWQGVPTADAFHDDSD
                     AAFTVDPDTHMLDYHVVWLQFTNQEQERWKMFPPLAGINVDDSQLRWNTQKSCGQLKN
                     RVQSQWMGSTILLKGSINAACTDQTMYSKGMVGDEFVYRTFKGLWQGMGGTGADGFRA
                     SATPISAGRILAKHQSQPVAKVVETMNKYSNNVIARSLILLADTGNKVGAEHALQRLQ
                     QKMNEDGINDGQLVLENGSGLSRRERVSARMMGSLLNKAYHSPYREAFWNSLPIAGVD
                     GTLRKRFKPLTGRLYLKTGTLDNVHALAGYGVDDAGQTVSIVVMVNGPDTPQTLIQMD
                     NLVTKIMAMPH"
     assembly_gap    2933..2942
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3025..3285
                     /gene="grxC"
                     /locus_tag="PK856_03780"
     CDS             3025..3285
                     /gene="grxC"
                     /locus_tag="PK856_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HRL01527.1"
                     /translation="MQKVIMYTTAQCPFCTMAKNLLKQKGVNEVEEIRIDTDPKMRDH
                     MMDITGRRTVPQIFIGDTHVGGFDDLNALNRADKLDALLAGE"
     gene            3310..3771
                     /gene="secB"
                     /locus_tag="PK856_03785"
     CDS             3310..3771
                     /gene="secB"
                     /locus_tag="PK856_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HRL01528.1"
                     /translation="MAEENQAVETQPVFSIEKIYVKDLSLEVPHAPQVFLANESPEVE
                     MKVATRGDKIEDGFYNVSVTVTVTAKLENEDKTMFLSEVTQSGIFRIDNIDDEDVNLL
                     LGVACPNILFPYAREAISTTVTRAGFPPVMLAPINFEAMYQSQGQVTEGNA"
     gene            3823..3993
                     /locus_tag="PK856_03790"
     CDS             3823..3993
                     /locus_tag="PK856_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01529.1"
                     /translation="MKTILCCVWKQAWAWFAMGIDTRKQAEYWQAACKPDWVFTGRLI
                     QCVNAIKQTEHA"
     gene            complement(3971..4732)
                     /locus_tag="PK856_03795"
     CDS             complement(3971..4732)
                     /locus_tag="PK856_03795"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRL01530.1"
                     /translation="MPWNPQIYDQFKAERQQPFFDLMAHIQAKPNLRIVDLGCGTGDL
                     TVCLAEHFSDADVLGIDSSLEMLQQAPSHPRVRFEHQNITDYLASGEAADVLVANASL
                     QWLDQHAQLWPQLRHKLRPHGQLAIQMPNQAHNCLNQLLSELAQQPRYAKSLRHWHRP
                     SSVLDLDDYAQILFAENAINMTLYQKVYPQIAASHDDLYQFIAGSALIPYLEHLPQNE
                     HAVFIADFKHSIANTFPQLPALYAFKRLIMHAQFV"
     gene            4903..5664
                     /locus_tag="PK856_03800"
     CDS             4903..5664
                     /locus_tag="PK856_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRL01531.1"
                     /translation="MQTVIQTKLQQTERERHIQILWAIESGSRAWGFASPDSDYDVRA
                     VYVQAPERYLQVDAAEEGFEWIEAEWFDVGAWDLRKTLRLLRSSNAVVLEWLQSPIIY
                     QGDLAFHAEIWALALAYFQPKAALYHYRGIAKTASAAFQADGSIRLKKWFYVLRPLLA
                     ALWVAQHQTIPPMTLEELLTGQDDDIKQRLWDLVAFKNEQSEAYVFTPDAVLQQLIAD
                     LWQQTEVALPERAVPDAEPLNRFFRTWVERYADPA"
     gene            5648..>6490
                     /locus_tag="PK856_03805"
     CDS             5648..>6490
                     /locus_tag="PK856_03805"
                     /inference="COORDINATES: protein motif:HMM:NF021631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRL01532.1"
                     /translation="MLTLPELEADNRHLLLRAISGSRAYGLDTPESDTDIKGVFVLPE
                     TDFYALNPLEQLNNPSNDVVYYEWRKFVALLHGSNPNILELLNTPEHALLYRHPLLEQ
                     LRPEWLLSKQCLHTFAGYAYGQIKKARGLNKKIVNPMSQEKKTVLDFCHVLQAAATVP
                     VTHWLQQQGWTETQIGLVKLNHAHDVYALFVDVEGTHGFHGIAQAESNSVRVSSVPVS
                     MPMQAYLSFNHDGYSSYLREYQSYWRWVGERNEVRYQTNLAHGAAYDSKNMMHTFRLL
                     HTALD"
BASE COUNT         1605 a         1523 c         1807 g         1545 t
ORIGIN      
        1 catcaccggc agcggcaaaa ccgaggtgta ttttgccgcc atggccgaag tgttggcgcg
       61 tgggcaacaa gtcttgatgc tgttgcctga aatcaacctc acgccgcaat tgcttgcccg
      121 catcaacagc cgtttttttg gctatgagcc tgcggtattg cacagtcaag cggcggtggg
      181 tgcgcgcagt catgattttg tgcgcgcgat gaaaggcgag gccaaactca tcatcggcac
      241 gcgtttgagc gtgctcacac ccatgcccaa tttgggcttg attgtggtgg atgaagagca
      301 cgatggctcg ttcaaacagc aaaacgaatt gcgctaccat gcgcgcgatg tggcgctgtg
      361 gcgtgccaaa caagcgggct gtcccatcgt gttgggcagc gccacgccca gtttggaaag
      421 ctggaacaat gcgattacag gccgctttgc attgttaagc ctgcctgaaa gggccaatgc
      481 gcaagcggtg ttgccaaaaa ttcacgtcat tgatgtgcgc caactgcctt tggaagaggg
      541 catgtgcgag cctgtgaagc aggcgctgct gaaaaactgg caacaaggcg gcatgtcgtt
      601 ggtgtatttg aatcggcgtg gctttgcacc tgtgttggcg tgtactgcgt gtggccacac
      661 ttttgattgc cccaattgct cggccaaaat ggtgtggcac caaaatcagc gccaattgcg
      721 ctgccaccat tgtgatttgc gcgagcctgt gcctgtgttt tgccccgaat gccacaacag
      781 cgatttgacc ccgcttgggc aaggcacaca gcgggtggaa gaggcgctca aggccttgat
      841 gccgcatgcc aatatcgttc gcgtagaccg cgacaccacc agtggcaaac aagattggca
      901 acagctgtat gagcaagtac aggccgatgc tgtggatgtg ttgataggga cgcaaatgtt
      961 ggccaaaggc catgattttg ccaagctcaa tttggtggtg attttgaatg ccgatggcgc
     1021 tttgtactcc agcgattttc gtgcgcccga gcatttgttt gcgcaattgt tgcaagtgtc
     1081 gggtcgggcg gggcgcgccg agcaaaaagg caaggtgttg ttgcaaaccc aattgccaga
     1141 ccatcctttg tttgcggcct tgcagcaaca cgattacatt ggttttgccg agcaagaatt
     1201 ggcttcgcgc caattgtatg aattcccacc ttttagccac atgctggcgg tgcgggtgga
     1261 tgcgccgcaa atgttggagg cacagcagtt attacaacag gtcttagacg aattggaact
     1321 gcctgacgac gtgagcgtgt ttggccctgt gcccatgttg atggcacgct tgaatgggcg
     1381 tgagcgcgcg caagtgtttg tgcaatcgcc gtcgcgacag tctttgcaca aggccgggcg
     1441 cttgtttcag gccgcattga gtgcgatatt gccacatcaa agcgcatggc gttatgctct
     1501 ggacatggat gtacaagatg cgtaaggcgg taaacacatg gggaacagtt tgaagaaaaa
     1561 acatctgcac ggggcttggc tggcgagcgc gttgttgtgg ggcagcgtcc acagcgcgtg
     1621 ggccttggat ttaagcggcc tgaaagccaa tgaggtggcg gtgtatgtgc aaaacatgga
     1681 cacaggccgc accgtgttgg aacaccgcgc cgatgcggcg atgaatcccg cctccaccat
     1741 gaaattgctc accgcttggt acagcctcaa ccgcctcagc cctgaatacc gcattcgcac
     1801 ctattggcac agcaaggcca agatagaaaa cggcgtgttg caaggcgatt tggtgtggcg
     1861 tggcagtggc gaccccactt tagaccacaa tgccttgcaa gaaatgatgc agcaattgca
     1921 agcagcaggg gtgaagcaca tacaagggca attggtattg gacaaaagcc tgtggcaagg
     1981 cgtgcccaca gccgatgcgt ttcacgacga cagcgatgcc gcattcaccg ttgaccccga
     2041 tacccacatg ctggactacc atgtggtgtg gttgcagttc accaatcaag aacaagagcg
     2101 ctggaaaatg tttccaccgt tggcaggaat caatgtcgat gacagccaat tgcgttggaa
     2161 cacccaaaaa agttgcggcc agttgaaaaa ccgcgtgcaa agccaatgga tgggcagcac
     2221 gattttactg aaaggcagca tcaatgcggc ctgtaccgac caaaccatgt acagcaaagg
     2281 catggtgggc gatgaatttg tgtaccgaac cttcaaaggc ttgtggcaag gcatgggtgg
     2341 tacaggcgca gatggttttc gcgccagcgc tacgcccatc agtgcggggc gcatcttggc
     2401 caaacaccaa tctcagcctg tggccaaagt ggtggaaacc atgaacaagt attccaacaa
     2461 tgtcattgcc cgcagtttga tattgttggc tgatacgggc aataaggtcg gagcggaaca
     2521 cgctttgcaa cgcttacagc agaaaatgaa tgaggatggt atcaatgacg ggcaattggt
     2581 gttggagaac ggttctggtt tatcacgccg tgagcgggtg agcgcgcgca tgatgggcag
     2641 tctgctcaat aaggcctacc acagcccata ccgcgaggcc ttttggaaca gtttaccgat
     2701 tgcaggcgtg gatggcacat tgcgcaagcg ttttaaaccg cttacaggcc gcttgtattt
     2761 gaaaacaggc acgttggaca atgtgcatgc actggcaggc tatggtgtgg atgatgcagg
     2821 gcaaaccgtg tccatcgtgg tgatggtgaa tggcccagat acgccacaaa ccctgatcca
     2881 aatggacaat ttggtgacta aaatcatggc catgccgcat taagacaggt gannnnnnnn
     2941 nngcattaac cagactgacc cacttgcaat ttgccccgtt tgcccctatc ttaaaaatca
     3001 tgttttgtta acgggacaga tgcgatgcaa aaagtgatta tgtatactac cgctcagtgc
     3061 ccattttgca ccatggcaaa aaacttgctc aagcaaaaag gcgttaatga agtggaagaa
     3121 attcgcattg ataccgatcc gaaaatgcgt gaccacatga tggacattac aggccgccgt
     3181 accgtgcctc aaatcttcat cggtgacacc catgtcggtg gtttcgatga tttgaatgcg
     3241 ctcaatcgcg ccgacaagct tgatgctttg cttgctggcg aataatttgt aacttagatg
     3301 gaaatccaaa tggcagaaga aaaccaagca gttgaaaccc aacctgtgtt cagcattgaa
     3361 aaaatttacg ttaaagattt gtctttagaa gtgccacatg caccgcaagt atttttagcc
     3421 aatgagtctc cagaagtgga aatgaaagtg gccacccgcg gcgacaaaat tgaagatggt
     3481 ttttacaatg tgagcgtgac tgtgaccgtg accgcgaaat tggaaaacga agacaaaacc
     3541 atgttcttgt cagaagtgac gcaaagcggt attttccgca tagacaacat cgacgacgaa
     3601 gacgtgaatt tgttgttggg cgtggcttgt cctaacatct tgttccctta tgcccgcgaa
     3661 gcgatttcta ccaccgtgac ccgcgctggt ttcccacctg tgatgttggc tccgattaac
     3721 ttcgaagcca tgtaccaatc acaaggccaa gtgactgaag gcaacgctta agttttacaa
     3781 ctaaaaacac catcttgcgc ggcaaggtgg tgttttttat gggtgaaaac catactctgt
     3841 tgtgtgtgga aacaggcttg ggcatggttt gctatgggta ttgatactcg gaaacaagca
     3901 gaatattggc aagcggcctg taaacccgat tgggtcttta caggccgctt gattcaatgt
     3961 gtgaacgcta tcaaacaaac tgagcatgca tgatgaggcg tttgaaggcg tacaaggcgg
     4021 gcagctgtgg aaatgtgttg gcgatgctgt gtttgaaatc ggcgatgaag acagcgtgtt
     4081 cgttttgagg caggtgctca aggtagggta tcaaggccga gcccgcaatg aattggtaca
     4141 aatcgtcgtg gctcgcggcg atttgtgggt agactttttg atataaggtc atgttgatgg
     4201 cgttttcagc aaacaggatt tgggcataat cgtccaaatc caataccgag cttggtctgt
     4261 gccagtgtcg caatgatttg gcgtagcgcg gttgttgtgc caattcgctc agcaattggt
     4321 tcaaacagtt gtgggcttga ttgggcattt gtatggccag ctgtccatgt ggacgcaatt
     4381 tgtgtctcaa ttggggccac aattgcgcgt gttggtctag ccattgtaag gacgcattgg
     4441 ccaccaacac atcagccgcc tcgccagagg caaggtagtc ggtgatgttt tggtgttcaa
     4501 agcgcacacg cggatgagac ggggcttgtt gcaacatttc gagcgagctg tctatgccca
     4561 aaacatcggc atcgctgaag tgttcggcca agcacactgt caagtcgcct gtgccgcagc
     4621 ccaaatccac aatgcgcaaa ttgggtttgg cttgaatgtg ggccatcaaa tcaaagaatg
     4681 gttgttggcg ctctgctttg aattggtcat agatttgcgg attccaaggc atgacggcat
     4741 ccatgaaaag tgatgggaaa gcaacaatat aaaacatcac acccctgctg caaagagggt
     4801 gttttaaaac ccaattctgc acgcaatatg cggcctgtaa gcacatcaaa acgcttacag
     4861 gccgcatctg ttatgatgct tacagtgaaa aaaggacatg ccatgcaaac cgtgatacaa
     4921 accaagttgc aacagacaga gcgcgagcgc cacatccaaa ttttgtgggc gatagaaagc
     4981 ggcagccgcg cgtggggttt tgcttcacct gacagcgatt acgatgtgcg ggcagtgtat
     5041 gtgcaagcgc ctgagcggta tttgcaagtg gatgcggctg aagaaggctt tgaatggata
     5101 gaagccgagt ggtttgatgt gggcgcttgg gatttgcgca aaaccttgcg cttgttgcgc
     5161 agctcgaatg cggtggtgtt ggaatggctg caatcgccca tcatttacca aggcgatttg
     5221 gcgtttcacg ctgaaatctg ggcattggca ctggcctatt tccaacccaa agccgcctta
     5281 taccattatc gtggcatcgc caaaaccgcc agtgcggcgt ttcaggccga tggatccatt
     5341 cgtttgaaaa aatggtttta tgtgttgcgg ccattgttgg cggctttgtg ggtggcgcaa
     5401 caccaaacca tcccacccat gactttggag gagttgttga cagggcaaga tgacgatatc
     5461 aagcagcgct tatgggattt ggtggcgttt aaaaacgagc agtctgaagc atatgtgttt
     5521 acgcccgatg cggtattgca acagttgatt gctgatttat ggcagcaaac agaagtcgct
     5581 ttgcctgagc gtgcagtacc cgatgccgag cctttgaatc gttttttccg tacatgggta
     5641 gagcgctatg ctgaccctgc ctgagctcga agccgacaac cgccatttgc tgttgcgcgc
     5701 catctctggc tcgagagcct atggcttgga cacgccagag tcagacaccg acatcaaagg
     5761 cgtgtttgtg ctgcccgaaa ctgattttta tgccctcaat ccgctagagc aactgaataa
     5821 tcccagcaac gatgtggtgt attacgaatg gcgcaagttt gtcgccttgt tgcatggcag
     5881 caatccgaat attttggaat tgttgaacac acctgaacat gccttgctgt atcgccaccc
     5941 attacttgag caattgcggc ctgaatggct gctctccaaa cagtgtttac acacgtttgc
     6001 aggctatgcc tatgggcaaa tcaaaaaagc gcgcggcttg aacaagaaaa ttgtgaatcc
     6061 aatgtcgcag gaaaaaaaga cggtgttgga tttttgccat gtgttacagg ccgctgctac
     6121 cgtgcctgta acgcattggc tgcaacaaca gggctggaca gaaacacaaa ttggcttggt
     6181 aaagctcaat catgcccacg atgtgtatgc gctgtttgtc gatgtagagg ggacgcatgg
     6241 ttttcatggc attgcacagg ctgagtcgaa ctcggtacgc gtgagttctg tgcctgtatc
     6301 catgcctatg caggcttatt tgagcttcaa tcacgatggc tacagcagct atttgcgcga
     6361 gtatcagtca tattggcgct gggtaggaga gcgcaatgag gtgcggtacc aaaccaatct
     6421 tgcccacggc gcagcatacg acagcaaaaa catgatgcac acgtttcgct tattgcacac
     6481 ggctttggac
//
LOCUS       DAOMVK010000072         6411 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_3249_length_6411_cov_3.191945, whole genome shotgun sequence.
ACCESSION   DAOMVK010000072 DAOMVK010000000
VERSION     DAOMVK010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 6411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6411
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3249_length_6411_cov_3.191945"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1375)
                     /locus_tag="PK856_03810"
     CDS             complement(<1..1375)
                     /locus_tag="PK856_03810"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRL01533.1"
                     /translation="MQLDIARLIDYFGGVNALAAAWKEHYPTEVVSTAAIYKWRARAS
                     LPLAQLQKLTALAELQGRPLDLNAFIQHQPQLERTTMSSNNRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRIARQLEKLGVDVIEAGFAAASPGDFDAIQTIAKTITKSTVCSLSRANE
                     RDVRAAGEAIQPAVKKRIHTFIATSPLHMEHKLKMTPQQVREAAVKAVKIACEYTDDV
                     EFSCEDALRSDIDFLVEICSDVIAAGATTINIPDTVGYAIPFQTEKFFRELISRTQGG
                     DKVVWSAHCHDDLGLSVSNSLAAVMGGARQVECTINGLGERAGNASLEEIVMALKTRQ
                     DVFGLETSIDATQIVPTSKLVSTITGYPVQPNKAIVGANAFAHESGIHQDGILKCRET
                     YEIMSAESVGWSHNRLTLGKLSGRNAFKTKLTELGIHLDTEEALNAAFARFKELADKK
                     REIFDEDL"
     gene            1520..2668
                     /locus_tag="PK856_03815"
     CDS             1520..2668
                     /locus_tag="PK856_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRL01534.1"
                     /translation="MSKNTSNLGLQQQQFDHQGQTLTWLSHSNIKPSLQLQFAGKHQT
                     LSAEQALKLWRDKTATVWQGDYHQAKALLDAVKRRLNKPLKTSNDILDTFHKWRLQQQ
                     QRSQLLSHLLVAVDANWQLNLARAPNIQAACEQAFGFRNENTVLLPLHALLGYMGAEQ
                     WHQRGVSIAALNGSNIHVPFGVFSPLRGEYLDLLSNTALPTTCQTAWDIGTGSGVLAA
                     ILAKRGIDHITATDTNPVAIATAAANFARLGVTEQISLCEQDLFPVGCADLIVCNPPW
                     LPAKPSSLIETAIYDPNSQMLRAFLSDAAAHLNEQGQAWLIMSNLAELIGLRGAEDLN
                     TWIADAGLRIVAKHDTAPKHGKAQDSSDVLHAVRSQEITSLFVLAKQA"
     assembly_gap    2836..2845
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2975..4501
                     /locus_tag="PK856_03820"
     CDS             2975..4501
                     /locus_tag="PK856_03820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRL01535.1"
                     /translation="MYHCKIATTAAEFRAIASLNYATFVEEIPQHPPNPDKVLVDKFH
                     DENTYLVFYQNEELIGMLAFRDVRPFSLDGKIGAMEQHLDADTCRKLCEIRLLAIKPA
                     HRNSFVFLRLAQALYAYILHQNYSACVISGTTRQQKLYHRIGFRQFAPEVGAAEARFQ
                     PMVLTANAARDFHAQFHRKQRIFYPGPVTQHTELSHTHVSHRSLLFDDLRAQTLSRLQ
                     QMSGAPKVALLLGSGTLANEMMLQQIKAKHSQALGLVCSNGEFGERLIKQAQALGLRF
                     QVYAEDWGQRFQAERLASHAQGVAWITAVHGETSTGCLNELSIFTDYKTQTHPECTLA
                     LDCVSSFGALPFSLKNVDFASATSGKALGALAGLSMVFYHQALAMQAEQGTYCQLAYY
                     DDQTPFTLPAYLLANVLAALTAYPQRYADLAQHLHTVLNHPLLQTHAIHTQHYPTAIS
                     YQLPSVWRQNAALNGLLLHQDSAYLQQRLWSQISTIQPDFAEDFAALDAWLQQQHLLT
                     "
     gene            complement(4575..5177)
                     /locus_tag="PK856_03825"
     CDS             complement(4575..5177)
                     /locus_tag="PK856_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01536.1"
                     /translation="MSDMWDTLRLRYRPFETYQFPAWVYVLALLLSGISMAAVMQSNA
                     NAQPEFSLPFVNQTGMVLYYVAFVVGQWWCLAYILPKILKHLGAEVPNMWGYMLVTQS
                     FSMFTILYTNLPKELMVVGSFFGVWTFWVQIFGLFNVVGRVISPWKVLLSYVLSMLSF
                     LLVLMLISSVFMIAGQMDTKELEHKMQQIMQEQQQLQQKK"
     gene            complement(5266..6117)
                     /gene="folD"
                     /locus_tag="PK856_03830"
     CDS             complement(5266..6117)
                     /gene="folD"
                     /locus_tag="PK856_03830"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HRL01537.1"
                     /translation="MSATILDGKAMSQQLLADVAAKVSQRSISGKRAPCLAVVLIGSD
                     AASAVYVNNKKKACEKVGIKSLSYEYGSEFTQAQLLALVDELNANDEVDGILVQLPLP
                     EHLDVQQIIERIRPDKDVDGFHPYNVGRLAVKMPLMRPCTPRGVMIMLEQSGVDVMGK
                     NVVIVGASNIVGRPQALEMLLARATITVCHSKTKDLAAEVGRADIVVAGVGIPNFIKG
                     EWIKPGAVVIDVGINRLETGKLCGDVEFAVAKERASMITPVPGGVGPMTIATLMQNTL
                     DACELHD"
BASE COUNT         1670 a         1741 c         1390 g         1600 t
ORIGIN      
        1 gcaagtcttc atcgaagatt tcacgctttt tatcggccaa ttctttaaag cgggcaaaag
       61 cagcattcaa tgcttcttcg gtatccaaat ggatgcccaa ttcggttaac ttggttttga
      121 aggcattgcg gcctgaaagc ttgcccaaag tcaggcggtt gtgtgaccaa cccacagact
      181 ctgctgacat gatttcgtaa gtttcacggc atttcaaaat accatcttgg tgaatgccag
      241 actcgtgtgc aaacgcattc gcaccaacga tggctttatt cggctgcaca gggtagccag
      301 tgatggtaga caccaattta gacgtaggca cgatttgcgt ggcatcaatg ctggtttcca
      361 agccaaacac atcttggcgg gttttcaaag ccatcacgat ttcttccaag ctcgcattgc
      421 ctgcacgctc gcccaagcca ttaatcgtac attccacttg gcgcgcaccg cccatcaccg
      481 ctgccaagga attggacaca gacaagccca aatcatcgtg gcaatgtgcc gaccaaacca
      541 ctttgtcacc gccttgggtg cggctgatca attcacggaa gaatttttcg gtttggaatg
      601 gaatcgcata gcccacagta tctggaatat tgatggtggt ggcacctgcg gcaatcacat
      661 cgctgcaaat ttccaccaag aaatcgatgt cagagcgcaa tgcgtcttcg caagagaatt
      721 ccacatcatc ggtgtattca catgcaattt tcactgcttt tacagcagct tcacgcactt
      781 gctgcggcgt catcttcagt ttgtgttcca tgtgcaatgg actggtggca ataaaggtgt
      841 ggatgcgctt tttaacagcg ggttgtatcg cttcacctgc cgcacgcaca tcgcgctcat
      901 tggcacgaga cagcgaacac acggtggatt tggtgatggt tttggcaatg gtttggatgg
      961 catcaaaatc gccaggactc gccgcagcaa aacccgcttc aatcacatca acacccaatt
     1021 tttctaactg gcgcgcaatc cggatttttt cttctttggt catggctgcg ccaggcgatt
     1081 gttcgccatc gcgcaaagtg gtatcaaaaa tgataacgcg gttattgcta gacatggtgg
     1141 tacgctccaa ttgaggttgg tgttgtataa aagcattcaa atccaaaggt ctgccctgta
     1201 gctcggccaa ggcagttaat ttttgtagct gcgccaatgg caaagatgca cgtgcacgcc
     1261 atttgtaaat ggctgctgtc gataccacct ctgtgggata atgctctttc cacgcagcgg
     1321 cgagtgcatt cacaccgcca aagtaatcga ttaaacgtgc aatatccagt tgcataatag
     1381 accttttgtc taaattagtt atcaaaactg ctaaatcagc aaaacgtgtc tccattgtag
     1441 tcacattttg gacaaaagca agactatttt gcagattttt tgcgattatt tcgcaatcta
     1501 caagcaagct ttatacataa tgtccaaaaa tacttccaat cttggtttac aacagcaaca
     1561 attcgaccac caaggtcaaa ccctgacttg gctcagccac agcaacatca agccgtcact
     1621 ccaattgcaa ttcgcaggca aacaccaaac cttgtctgct gaacaggctt taaaactgtg
     1681 gcgtgacaaa accgccaccg tgtggcaagg cgattaccac caagccaagg ccttgcttga
     1741 tgcggtcaaa cgccgtttga acaagccctt aaagacttct aacgatatct tagacacgtt
     1801 tcacaaatgg cgtttgcagc aacaacagcg cagccaattg ctaagccatt tattggtggc
     1861 agtagatgct aattggcaat tgaatcttgc ccgcgcgcct aacatacagg ccgcttgtga
     1921 gcaagcattt ggctttcgca atgaaaatac agtgttattg cctctacatg cactgctcgg
     1981 ctacatgggt gcggagcagt ggcatcagcg cggtgtgagc atagcggcat tgaatggcag
     2041 caacatacac gtaccctttg gggtgttttc accactgcgc ggcgaatatt tggacttgtt
     2101 gtccaacaca gccttgccaa caacatgtca aactgcttgg gacataggca caggttcagg
     2161 tgtactggca gcgattttgg ccaagcgtgg catagaccac atcaccgcca ccgacaccaa
     2221 ccctgttgcc attgccacgg cggccgccaa ttttgcacgc ttgggtgtga ctgaacagat
     2281 tagcttgtgt gaacaagact tatttcctgt aggatgtgcc gatttgattg tgtgtaatcc
     2341 gccatggctg cctgccaaac ccagctcact gattgaaacc gcgatctacg accccaacag
     2401 ccaaatgctg cgcgccttct taagcgacgc cgccgcacat ttgaacgaac agggtcaagc
     2461 ttggctcatc atgtctaact tggccgaact cataggcttg cgcggtgcag aagatttgaa
     2521 cacttggatt gcagacgcag gtttgcgcat agtggccaag cacgacactg ccccgaaaca
     2581 tggcaaggcg caagacagca gcgacgtgtt gcacgcagta cgctcccaag aaatcacgag
     2641 tttatttgta ttggccaaac aagcttaaac aacgctgtca tagcaagcgg ccagtaagac
     2701 tcataccagt cttactggcc gcttgtgctc aattgcacaa cttttgtcaa aatttagggt
     2761 gagtacaaag cctataacac accatcatgc taagctaaag cccatcttgt cagccgagct
     2821 tgcccatgta ccactnnnnn nnnnnaccat aaagtggttt acaggccgct ttttgcagtg
     2881 tgattgcgca gcttgtgtca aaaatcgcgg gcaaccaagc ccatcacatg ccagcgtgtt
     2941 aagctaaggc caatcttgtc cgccgagctt gcccatgtac cactgcaaaa ttgccaccac
     3001 cgcagccgaa ttccgtgcca ttgcgagctt gaattacgcc acttttgtgg aagaaattcc
     3061 gcaacacccg cccaatccag acaaagtctt ggttgacaaa tttcatgacg aaaacaccta
     3121 tttggtgttt tatcaaaatg aagaactcat aggcatgttg gcgtttcgcg atgtgcgccc
     3181 tttctcgctc gacggcaaaa ttggcgccat ggagcagcat ttggatgccg acacttgccg
     3241 taagttgtgc gaaattcgcc tgctcgccat caaacccgcc caccgcaaca gcttcgtgtt
     3301 tttgcgcttg gctcaggctt tgtatgctta catccttcac caaaactaca gtgcctgcgt
     3361 gatttcaggc accacgcgcc aacaaaaact gtatcaccgc atcggctttc gccaatttgc
     3421 acctgaagtc ggcgcagccg aagcccgttt ccaacccatg gtcttaaccg caaacgccgc
     3481 ccgcgatttt catgctcaat tccatcgcaa gcagcgcatt ttctacccag ggccagtgac
     3541 gcaacacaca gaactgagcc acacccatgt gtcacaccgc tctctcctgt ttgatgattt
     3601 acgggcacaa accctgtctc gtttacaaca aatgagcggt gcgcccaaag tggctttatt
     3661 gctcggcagc ggcactctgg ccaatgagat gatgttgcaa caaatcaagg ccaaacatag
     3721 tcaagcttta ggattggtgt gcagcaatgg tgaatttggc gaacgcttaa tcaagcaggc
     3781 acaggctttg ggcttgcggt ttcaggtgta tgcagaagat tgggggcagc gttttcaggc
     3841 cgagcgcctg gccagtcatg cccaaggtgt cgcttggatt acggctgtgc atggagaaac
     3901 atcgacaggc tgcttgaatg agctatcaat atttaccgac tacaaaaccc aaacacaccc
     3961 cgaatgcacc ttggccttgg actgtgtttc ctcatttggc gccctgcctt tttccttaaa
     4021 aaatgtcgac tttgcctcgg ccacatcagg caaagccttg ggtgcattgg caggcttgag
     4081 catggttttt taccaccaag cgcttgccat gcaagccgaa caaggcactt attgccaatt
     4141 ggcctattac gatgaccaaa ccccatttac cctgcccgcg tatttattgg ccaatgtgtt
     4201 ggcggctttg acagcctacc cgcaacgcta tgccgatttg gctcagcatt tacacacggt
     4261 tctgaatcat cccttgttgc aaacccatgc cattcacacc caacactacc ccactgccat
     4321 cagttaccaa ttgccttctg tatggcggca aaatgccgct ctgaatggct tattgctgca
     4381 ccaagacagc gcttatttgc aacagcgctt atggtcgcaa atcagcacca tacaacctga
     4441 tttcgctgag gattttgctg ctttggatgc gtggttgcag cagcaacact tactcacata
     4501 atcaacctta cctgctccca atcaaatacg gcctgtaaat gtttacaggc cgcttgcatg
     4561 atgcattgtt tggattattt cttttgttgc aattgctgct gttcttgcat gatttgctgc
     4621 attttatgct ccaattcttt ggtatccatt tgtccagcaa tcatgaacac gctgctgatg
     4681 agcatcaata ccagtaaaaa cgacaacatg gacaacacgt aagacagcaa aactttccac
     4741 ggtgaaatca cgcgccccac cacattgaac aaaccaaaaa tttgcaccca aaatgtccac
     4801 accccaaaaa agctgcccac taccatcagc tcttttggca aattggtgta caaaatggtg
     4861 aacatggaaa atgactgcgt caccaacata tagccccaca tattgggcac ttcggcacca
     4921 agatgcttga gtatcttggg caaaatgtag gccaaacacc accactgccc caccacaaac
     4981 gccacataat acaaaaccat gccagtttga ttgacaaatg gcaggctgaa ttcaggctgc
     5041 gcatttgcgt tgctttgcat caccgccgcc atgctgatgc ctgacaacaa caaggccaac
     5101 acatacaccc aagcaggaaa ttggtaagtc tcaaatggac ggtagcgcaa gcgcagcgtg
     5161 tcccacatat cactcaaaag ctgattcatt ggcacttatc tgtagtaaat aaaacggcaa
     5221 aaagaaaagc ttacaggccg gcctgtaagc tcatgtcaac acacatcaat cgtgcaattc
     5281 acacgcatcc aaagtgtttt gcatcaaggt ggcaatggtc ataggcccga caccacctgg
     5341 cacaggggta atcatgctgg cgcgctcttt ggccacagca aattccacat caccgcataa
     5401 tttacccgtt tccaaacgat taatgcccac atcaattacc accgcacctg gcttgatcca
     5461 ttcacctttg atgaaattgg gtatgcccac gcctgccacc acgatgtcgg cacgacccac
     5521 ttcggcagcc aaatctttgg ttttagaatg acacactgtt atcgttgcac gcgccaacag
     5581 catttccaag gcttgtggac ggccgacaat attcgacgca cccactatca ccacattttt
     5641 acccatcaca tccacgccag attgttccaa cataatcatc acgccgcgcg gtgtgcaagg
     5701 gcgcatcaat ggcattttca ccgccaaacg tcccacgttg taaggatgga atccatccac
     5761 atctttgtca gggcgaatgc gctcaatgat ttgctgcaca tccaaatgtt ctggcaatgg
     5821 caattgcacc aaaataccat cgacttcatc attggcattc aattcatcca ccaaagccaa
     5881 caattgcgct tgggtgaatt cgctgccata ttcatacgac aaggatttga tgcccacttt
     5941 ttcgcaggct tttttcttat tattgacata tacagcactg gcagcatcac ttccaatcaa
     6001 taccactgcc aaacacggcg cccgtttgcc agagatactg cgttggctca ctttggctgc
     6061 cacatcggcc aacaattgtt gtgacatggc tttgccgtct aatatcgtcg cactcatgaa
     6121 tccatctttc ttaaataagg tgcacaccgc acccaagcaa aggcggtatt ttgccatttt
     6181 ttgaggtttt tcgcacctac caattccaga aaaaagccaa gtgtttgttc aaacacctgc
     6241 cattgtgcgt ttccgcacaa gaaaaatacc actgtttatc aaacccctat tcaattgcgc
     6301 ccattttctt ccgcgacaac gcttgacact ttctgagcag ctgcgtatag ttcggcttct
     6361 cagtcggggc gtagcgcagc ctggttagcg catctgcttt gggagcagag g
//
LOCUS       DAOMVK010000073         6316 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_3312_length_6316_cov_3.112762, whole genome shotgun sequence.
ACCESSION   DAOMVK010000073 DAOMVK010000000
VERSION     DAOMVK010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 6316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6316
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3312_length_6316_cov_3.112762"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="PK856_03835"
     CDS             <1..1057
                     /locus_tag="PK856_03835"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRL01538.1"
                     /translation="IFKVIPNHNLDIRYRPAALWHFKNPLMQYWYYSDPKRFKTVVAE
                     WSTLIEHCTETHDEMMQAAHAEHLVRKTGWLQLHRTTETYAKAIAEAEATRAYGVQSR
                     LVNMQEVHEMEPDLNPSLFVGGIHWQNTWKVTNPSALVKAYVENFVAAGGEFKQCQMY
                     NMQQENGEWQVNTDQGRLSAKHLVVATGPWSADVLKGLGYDYPLFPMRGYHKHYNIAE
                     GKQLHHSVLDYDCGYVIGPKAAGIRITTGAEFTFQNAPADERQLDDVEKKAREYLPLT
                     TAVEDKAWIGHRPCMPDMKPVIGPAHKHGNLWFAFGHAHQGFTLGPATGKLLTQMMLG
                     QATYVNPQPFAANRFEK"
     gene            complement(1148..1411)
                     /locus_tag="PK856_03840"
     CDS             complement(1148..1411)
                     /locus_tag="PK856_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01539.1"
                     /translation="MNKEKKMLLIGIVLLALGLVLKFALPDSSAENLAIAQQAQSAQE
                     AAQAISSNNQSDILTNSLGMFLLGLGGVLTVVGGLKVVSSRKA"
     gene            complement(1509..2099)
                     /locus_tag="PK856_03845"
     CDS             complement(1509..2099)
                     /locus_tag="PK856_03845"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRL01540.1"
                     /translation="MYLWNLQPIKAVVFDAFGTVIDYPVKLSPYALLRRHWRSEQGFK
                     HACLTQNIALPDLLQQLELDALWPEMQSILTEERKHLALYPEVAEVMQKLQQLQVKTA
                     ICSNLAHAYGADVQRLLPDVDATILSYEVGHAKPEMAIYAVVEQQLNLPKEQILFVGD
                     TQRCDYDGPKAYGFQAAHLQRRLGQDLYSILFPSTH"
     gene            complement(2159..2626)
                     /locus_tag="PK856_03850"
     CDS             complement(2159..2626)
                     /locus_tag="PK856_03850"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRL01541.1"
                     /translation="MDLILWRHAEAREGSPDLTRPLTRKGCQQAKEMAEFLNLHLAKN
                     TQVWTSEATRSIETAAYLNRPSQLQAALNPTADYSDILPLLMAEHKQPLLVVGHQPWL
                     GQVWEHCMTGGVGSEDYWTVKKGAVVWLKMKMQADGLDCKLVAALSPVFLGVI"
     gene            2681..3187
                     /locus_tag="PK856_03855"
     CDS             2681..3187
                     /locus_tag="PK856_03855"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: protein motif:HMM:NF016248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HRL01542.1"
                     /translation="MKPLNTWLSLENGFVLDDNMAYLSDILQAIPLTLALKAKCPQFS
                     VDLLHLGGSEIEQDENVYVNTDQGYSREVALCLNQDAVVWARSVCAATDAAWHEIMDC
                     GTMPLGMRLYELPLERTDFEYAYLSPEHPANPSDDIIVSRRSCFYLQAAPLLLIESFL
                     PDLKRQYE"
     gene            3180..4064
                     /gene="ubiA"
                     /locus_tag="PK856_03860"
     CDS             3180..4064
                     /gene="ubiA"
                     /locus_tag="PK856_03860"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HRL01543.1"
                     /translation="MNDTVKMNWQQKLQVYGRLMRVEKPIGTLLLLWPTYWALLIASD
                     GSPDWLMVLVFTLGTFLMRSAGCVINDYADRDIDGAVARTKNRPFAMGQVGKKEALLL
                     TLILCLLAVMCLLPLNTLTWWMSLPAVFLALTYPFTKRFFPIPQLYLGFAFSFGVPMA
                     FAAETNAVPALAWLIFTANVLWTIAYDTCYAMADKEDDLKLGNIKTSAITFGDHDITA
                     VMICHGLFVFLMALVGWNIDATWSYWLALIITMAMQYWQYTQIKGRDRQKCFEIFLKN
                     NRIGWVISLGLLLHYWWS"
     gene            4158..4607
                     /locus_tag="PK856_03865"
     CDS             4158..4607
                     /locus_tag="PK856_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003743926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRL01544.1"
                     /translation="MNVIGEILPLDHIVLDLPVSSKKRLFEQIGLLIENKAEMARSDV
                     FECLFAREKLGTTALGQGVAIPHGRYAGLKRPIGAFIRTAEPIGFDAPDGKPVNLIFA
                     LLVPEGSNSVHLEVLSHLASRFSSKAVREQLLQANDTQAVQNILISE"
     gene            4619..5581
                     /gene="hprK"
                     /locus_tag="PK856_03870"
     CDS             4619..5581
                     /gene="hprK"
                     /locus_tag="PK856_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HRL01545.1"
                     /translation="MPSISVRRLYQDNQSKLKLAWAAGTAGIDNRIGIEADKPLLALV
                     GHLNFIHPNQVQVLGSAEVKYLNDLEASKIRTSLNELFGKTIAVVIVANDLAVPFLLR
                     DYCHTHNVPLLTSKLESPYLMDVLRIYLQRALAASTVMHGVFLDVFEVGVLLMGKSGL
                     GKSELALELISRGHGLVADDAVEIYRIGPETLEGRCPPVLRDFLEVRGLGVLNIRAIF
                     GETAIRPKKPLKLIINLVSADDQLMQSLDRLSIQSEKQDILNVHIRKVSIPVAAGRNL
                     AVLVEAAVRNFILQSRGLDSTKQFIERHTQVLKEDQLNDESHSD"
     gene            5622..>6316
                     /gene="rapZ"
                     /locus_tag="PK856_03875"
     CDS             5622..>6316
                     /gene="rapZ"
                     /locus_tag="PK856_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HRL01546.1"
                     /translation="MEDLGFFCVDNLPLELLPQLVTLYQAKPGVTDLAVCLDMRTRIS
                     QQYFLPMLEAIEHAVSRFDVLFLEAQKEVLLHRFSETRRRHPLTNDDCTLEEALAYER
                     EWLMPLRERSHVVDTTLLTTPKLRQYITSWVGLPNRNLQIVFESFGFKHGMPSDADFM
                     FDVRSLPNPHYEPAIKHLTGKDAEIQAYFAGYPQVQAMIDDIAQFLCKWLPSIDQENR
                     SYVTIAIGCTGGQ"
BASE COUNT         1622 a         1478 c         1543 g         1673 t
ORIGIN      
        1 catattcaaa gtcatcccca atcacaattt ggacatccgt taccgtcctg cggctttgtg
       61 gcattttaaa aatccgttga tgcaatattg gtattactcc gatcctaagc gtttcaaaac
      121 cgtggtggcg gaatggtcaa ccttgattga gcattgcacc gaaacccacg atgagatgat
      181 gcaagcagcg catgcggagc atttggtgcg caaaacaggc tggttgcaat tgcaccgcac
      241 caccgaaacc tatgccaaag ccattgctga agcggaagcc acccgtgctt atggcgtgca
      301 atcgcgcttg gtaaacatgc aagaagtgca tgaaatggaa cctgatttga atccgagttt
      361 gttcgtgggc gggattcatt ggcaaaacac ttggaaagtg accaatccaa gcgctttggt
      421 caaagcctat gtggaaaact ttgtggccgc aggcggtgaa ttcaaacaat gccaaatgta
      481 caacatgcag caagaaaacg gcgaatggca ggtgaatacc gatcaaggcc gcttgagcgc
      541 gaaacacttg gtggtggcaa ctggcccatg gtctgccgat gtgctcaaag gcttgggcta
      601 cgattaccca ttgttcccga tgcgtggcta ccacaagcat tacaacattg ccgaaggcaa
      661 acaattgcat cacagcgttc tcgattacga ttgtggctat gtcatcggtc cgaaagcggc
      721 gggcatacgc atcaccacag gcgcggaatt caccttccaa aatgcccctg cggatgagcg
      781 ccaattggac gatgtggaga aaaaagcgcg tgaatacctg cctttgacca ccgccgtgga
      841 agacaaagcg tggataggcc atagaccgtg catgccagac atgaaacccg tcatcggccc
      901 tgcacacaaa cacggcaatt tgtggtttgc ctttggccat gcccaccaag gcttcacctt
      961 gggccctgct acgggcaaat tgctgacaca aatgatgttg ggacaagcga cttatgtgaa
     1021 cccacagcct tttgcagcca accgttttga gaaataaacg cttttagtga aaatgaaatg
     1081 agaaaagcgg cctgtaaacg tttacaggcc gctttttatt gttaattaag gctgttaaat
     1141 aaagccatta cgccttgcgg gaagacacca ctttcaagcc acctacaacg gttaataccc
     1201 cacccaagcc caataaaaac atgcccaagc tgttggttaa aatatcgctt tggttattgc
     1261 tggatatggc ttgtgccgct tcttgcgcac tttgtgcctg ttgggcgatg gccaaatttt
     1321 cagcgctgct gtcgggcaaa gcaaacttca agaccaaacc caatgccaac aacacgatac
     1381 cgatgagcaa cattttcttt tctttgttca tgtaagacag tccagtttca aaacaagcgc
     1441 cattctaaaa accccgcaac catctgtcaa aacagcttgg gtaagcatgt tgcgcctgag
     1501 agaggtattc agtgcgttga cgggaataga atgctgtaca aatcttgacc caaacgccgt
     1561 tgtaaatggg cggcctgaaa gccataagcc ttaggcccat cgtaatcgca gcgctgggta
     1621 tcgcccacaa acaaaatctg ttccttgggc agattcaact gttgttccac cacagcataa
     1681 atggccatct caggtttggc atggcccact tcataactca aaatcgtagc atccacatcg
     1741 ggcaataaac gctgcacatc agcgccataa gcatgggcaa gattgctgca gatggcggtt
     1801 ttaacctgta attgctgcaa tttttgcatc acttcagcca cttcagggta caaagccagg
     1861 tgttttcgtt cctcagtcaa tatagactgc atttcaggcc acagtgcatc gagttccaat
     1921 tgttgcaaca aatcgggcag ggcgatattt tgagtcaaac aggcatgttt gaagccttgt
     1981 tctgagcgcc aatggcgacg caataaggca taaggactga gtttgacagg ataatcaatg
     2041 acggtgccaa acgcatcaaa caccactgct ttgatgggct gaagattcca taagtacata
     2101 cacacacttt ctatcaagcg gcctgtaaac caatattcaa gctgccatca cgttgaaatt
     2161 aaatcacgcc caaaaacaca ggcgacaaag ccgccaccag tttgcaatcc aagccatcgg
     2221 cttgcatttt catctttaac caaacaacag cgcctttttt gactgtccaa tagtcttcgc
     2281 tgcctacacc acctgtcatg cagtgttccc aaacctgacc caaccacggt tggtgcccca
     2341 ccaccaataa gggttgtttg tgctctgcca tcaacaaggg cagaatgtcg ctgtagtctg
     2401 ctgtgggatt gagcgcggcc tgtaattggc ttgggcgatt caagtaggcc gcggtttcta
     2461 tgctgcgggt ggcttcggaa gtccaaactt gggtgttttt cgccaagtga aggttcaaaa
     2521 actctgccat ttccttggct tgttggcagc ctttgcgcgt taaggggcga gtcaagtcgg
     2581 gactgccttc tcgtgcctca gcatggcgcc ataaaatcaa atccatgttt gctccaatag
     2641 gggtttgcgt tgcctgattt gcatgggcat aatgcgcgtc atgaaacctt taaatacttg
     2701 gctatcgctt gaaaacggct ttgtgttaga tgataacatg gcttatcttt ccgacatatt
     2761 acaagccata ccattgactc tggcattgaa ggccaaatgc ccacaattca gcgtggattt
     2821 gttgcatttg ggtggcagtg aaatagagca agatgagaac gtttatgtga acacagacca
     2881 aggttacagc agagaagtag ccttgtgttt gaaccaagac gcggtggttt gggcccgcag
     2941 tgtgtgtgca gccactgatg cggcttggca tgaaatcatg gattgcggca ccatgccttt
     3001 gggcatgcgt ttgtatgaat taccgctgga gcgtaccgat tttgaatacg cttatttaag
     3061 ccctgagcat cctgccaatc ccagtgatga catcatcgtg tcgcgccgct cgtgttttta
     3121 tttacaggcc gctcccttat tgttgattga aagcttttta cccgatttaa aacgtcaata
     3181 tgaatgatac tgtgaaaatg aattggcagc agaagctgca agtatacggt cgcttgatgc
     3241 gagtagaaaa acccataggc actttgctgc tgttgtggcc aacgtattgg gccttgctga
     3301 ttgcgtctga tggcagccca gattggctga tggtgttggt gtttaccttg ggcacgtttt
     3361 tgatgcgcag tgcgggttgt gtcatcaatg actatgccga tcgcgacatt gatggtgcgg
     3421 tggctcgcac caaaaaccgt ccgtttgcaa tgggtcaagt cggtaagaaa gaagccttgt
     3481 tgttgacgct gatattgtgt ttgttggcgg tgatgtgttt attgccattg aacacattga
     3541 cgtggtggat gagtttgcct gcggtgtttt tggccttgac ctatccattc accaaacgct
     3601 tttttccaat accgcaattg tatttgggct ttgccttttc attcggcgtg cccatggcgt
     3661 ttgcagctga aaccaatgcc gtgcctgcgt tggcgtggtt gattttcact gccaatgtgc
     3721 tttggaccat tgcttacgat acttgctacg ccatggccga caaagaagac gacttgaaac
     3781 tcggcaacat caaaacctca gccatcacgt ttggcgacca tgatattact gcggtcatga
     3841 tttgtcatgg tttgtttgtg tttttaatgg ctttggtggg atggaacata gacgccactt
     3901 ggtcatattg gctcgcttta atcatcacca tggccatgca atactggcaa tacacgcaaa
     3961 ttaaagggcg tgatcgccag aaatgctttg aaatattctt aaaaaacaac cgcattggtt
     4021 gggtaatcag cttagggttg ttgctgcatt attggtggtc gtgataacgt ctttttaccc
     4081 taaagtcaac attgtcgcgc cgccatttag cgcgtaaaat ccgaaacgca tcgtaaagaa
     4141 agtggaaaat aatggtaatg aatgtgatag gcgaaatctt gcctctcgac cacatcgttt
     4201 tggatttacc ggttagcagt aaaaaacgtt tgtttgaaca aattggcttg ttgattgaaa
     4261 acaaagccga aatggccaga agtgacgtgt ttgaatgttt gttcgcccgt gaaaagttgg
     4321 gcaccaccgc tttgggtcaa ggcgttgcca tcccacatgg taggtatgca ggcttgaagc
     4381 gcccgatagg tgctttcata cgtacagctg agcccattgg ttttgatgca ccagacggca
     4441 agcctgttaa tctgattttt gctttattgg tgccagaagg ctctaacagc gtgcatttgg
     4501 aggtgttgtc ccatttggcc agtcgtttct cttcaaaagc agtgcgagaa caattattac
     4561 aggccaatga cacccaagcg gttcaaaaca tcttaatttc agaataaatg gagtgaccat
     4621 gcccagtatt tccgtgcgac gcctctacca agacaatcaa agcaaactca aattggcttg
     4681 ggcggcaggc acagcgggca tagacaatcg cattggcatc gaagccgata agcctttatt
     4741 ggctttggtt ggccatctta atttcataca ccccaatcaa gtgcaggttt tgggctcagc
     4801 cgaagtcaaa tacctgaatg acttggaagc cagtaaaatt cgcaccagtt tgaacgaatt
     4861 gtttggcaaa accatcgccg tggtaatagt cgcgaatgat ttggcagtgc catttttgct
     4921 gcgtgattat tgtcacacac acaacgtgcc tttgctcacc tccaagctgg aaagcccgta
     4981 tttgatggat gtgttgcgaa tttatttgca acgcgctttg gctgcatcca ccgtcatgca
     5041 tggtgtattc ttggatgttt ttgaagtggg cgtgttgttg atgggcaaat caggtttggg
     5101 taagagtgag ttggctttgg agttgatttc acgtgggcat ggtttggtgg ctgatgatgc
     5161 cgttgaaatt taccgcatcg gccctgaaac cttggaaggg cgttgcccac ccgtgttgcg
     5221 cgatttttta gaagtacgcg gcttgggtgt gttgaacatt cgtgccatct ttggtgaaac
     5281 cgccatccgt cccaagaagc cattgaagct catcatcaat ttggtcagtg ccgacgacca
     5341 attgatgcaa agcttggaca gactcagtat ccaatccgaa aaacaagaca ttttgaatgt
     5401 ccacatacgc aaagtcagca ttcctgtggc agctggccgc aacttggcag tgttggtgga
     5461 agcagcggtg cgcaatttta ttttgcaaag ccgtgggctc gacagcacca aacaattcat
     5521 cgaacgccac actcaggttt tgaaagagga tcagttaaac gatgagagtc attctgatta
     5581 gtggtttgtc cggttcaggt aaatcggtcg cgcttaagtt aatggaagac ttaggctttt
     5641 tttgtgtcga caatttaccg ctggaattgc tgccacaact ggtaactttg taccaagcca
     5701 aacctggtgt gacagatttg gctgtttgtc tggacatgcg cacccgcatc agtcagcaat
     5761 acttcttgcc catgcttgaa gccatagagc atgcagtgtc acgctttgat gtgttgttct
     5821 tagaggcaca aaaagaggtg ttattacacc gtttttctga aacccgccgc cgccatccct
     5881 taaccaatga cgactgtact ttggaagaag cattggctta tgaacgcgaa tggctgatgc
     5941 cgctacgtga gcgctcacac gtggtagaca ccacgttgct gaccacgcca aaattacgcc
     6001 aatacatcac atcgtgggtt ggtttgccta accgcaactt gcaaatagtg tttgaatctt
     6061 ttggttttaa acatggcatg cccagtgatg ccgattttat gttcgatgtg cgcagcttac
     6121 cgaatccaca ttacgaacct gccatcaaac atttaacggg caaagatgcg gaaattcagg
     6181 catatttcgc aggctatccg caagttcagg ccatgataga cgacatcgcc caatttctgt
     6241 gcaaatggct gcccagcata gaccaagaaa accgcagtta tgtcaccatt gccatcggtt
     6301 gcacgggcgg acaaca
//
LOCUS       DAOMVK010000074         6289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_3330_length_6289_cov_4.344562, whole genome shotgun sequence.
ACCESSION   DAOMVK010000074 DAOMVK010000000
VERSION     DAOMVK010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 6289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6289
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3330_length_6289_cov_4.344562"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="PK856_03880"
     CDS             <1..729
                     /locus_tag="PK856_03880"
                     /inference="COORDINATES: protein motif:HMM:NF024293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
                     /protein_id="HRL01547.1"
                     /translation="GSTQGIDNLLTAYKKQNLNSGTPKDPIMYLNTSMLRNMLGNLDS
                     GTKIKVGGDYAALEAAATKVLATGTQLKQQADSLEAYFKSKKYLDDGYAKSKAENAAF
                     EKQWEQFNADYEVFNTELSKVEKVQRLASIESFTKAGKLREAAQEKALMSANEVLETL
                     SSVEDLNNAEKMATADAEMQKLEAALTELNDANGKTKDSDSYKFEGTFDYLTSFTGYW
                     REMKAEKDPSRYERMVDSYNSAVR"
     gene            complement(816..1589)
                     /locus_tag="PK856_03885"
     CDS             complement(816..1589)
                     /locus_tag="PK856_03885"
                     /EC_number="3.5.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR04448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047789 - creatininase activity [Evidence
                     IEA];
                     GO_process: GO:0046449 - creatinine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase"
                     /protein_id="HRL01548.1"
                     /translation="MSYLLNEISWYDYQDKIKNQHILIIPIGATEQHGPHLPLGVDAM
                     LAKAFSLALAKQINGVVAPTLNYGYKSLPLSGGGPFFPGTIDLNGNTLIQLTKDIVNE
                     FARDGWKHIVIFNCHYENEAFVLEGADLASKEAEFNSQIFIVNWWDNLDSHIVDQVFN
                     QVPFPGWALEHAAISETSLMMYFHPNLVDSTKIPNDHIEELLHFHSYPPLEGVIPATG
                     CLHTSHSSSSEKGELIVNNVIHNIVKHMIPRFKIVVLTH"
     gene            complement(1611..2990)
                     /locus_tag="PK856_03890"
     CDS             complement(1611..2990)
                     /locus_tag="PK856_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRL01549.1"
                     /translation="MSQELKKSLQLRHLTMIAFGGSIGAGLFVGSGALIKLTGPAAVV
                     TALIAGLLVIMVMRMLGEMVVAKPSLGSFSDYAREALGNWAGFSVGWLYWYFWVIVIA
                     VEAIAGAAILKDWLFPDTSLWVLNLSIMVLLTITNLFSVRAYGEFEYWFSLIKIVAIV
                     LFLICASAFVLGLLPNTKMDFSNLTAHGGFAPLGYSAAFLAVATIIFSFVGSEVVTIA
                     AAESVESERAVVRAVNSVIVRIMTFYVGSVFLVVMIVPWTDHQALVNPYVSALKTIGI
                     PYADKIMNLVIVTAVLSCLNSGIYTASRMLFALADKQDAPQAIKKLTNNGVPVRAILT
                     CTVFGFVSVAMAFVSPDTVFQFLLNSSGAIAIFVYLLIAISQLILRKKYERECPEKLK
                     IRMWCFPYLTIFTIVSLCLIILTMAYNPDTRSQLFLSVVSFAVVIILYRIKTVVQSRN
                     QKNDALTKV"
     gene            3981..>6289
                     /locus_tag="PK856_03895"
     CDS             3981..>6289
                     /locus_tag="PK856_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HRL01550.1"
                     /translation="MQSTPDTQAINNALSLDDKFTQDHGRIYISGIQALVRLPMLQKQ
                     RDARAGLNTAGYVSGYRGSPLGGVDLALWQAKEHLQKNDIVFQPGLNEDLAATAVWGT
                     QQTNLYETATRDGVFGMWYGKGPGVDRSMDVLKHANAAGTSPHGGVLVLAGDDHAAKS
                     SSVAHQSEHVLLAAGIPVLYPSNVQEYLDFGLHGWAMSRYSGLWVAMKCVTDVVESSA
                     SVEVNPERVDIKIPTDFQMPADGLNIRWPDTPLAQEQRLYDYKWYAALEYIRLNKLNR
                     IEIDAPQARFGIMTAGKAYLDVRQALADLGLSDEVCEKIGIRLFKVGCVWPLDARDAR
                     EFATGLDEILVVEEKRQILEYALKEELYNWREDVRPSVYGKFDERDHAGGEWSQPRGQ
                     WLLPARYELSPAIIAKAIASRLLRMDLPEDIVAKINARLDVIRAKEQEAKVPRITVER
                     KPWYCSGCPHNTSTKVPEGSRAMAGIGCHYMVMWMDRNTETFTQMGGEGVTWLGQMHF
                     TRDKHVFVNLGDGTYFHSGLLAIRASIAAKANITYKILYNDAVAMTGGQPVDGVLTVP
                     QIAQQVLAEGAAKLIIVSDEPEKYAERMGIPDSVTIHHRDEFDGLQKQMREVEGVSII
                     IYDQTCATEKRRRRKRGTYPDPAKRVFINTDVCEACGDCSVQSNCLSIEPKPTSHGNK
                     RQINQSTCNKDFSCVKGFCPSFVTLNGAQLRKPKVSEQKFDVAALPEPVLPKLDHAYG
                     IVITGVGGTGVITVGALLGMAAHLEQKGVT"
BASE COUNT         1817 a         1410 c         1544 g         1518 t
ORIGIN      
        1 ggcagcaccc aaggcataga caatttgctc acggcgtaca aaaagcaaaa tctgaactca
       61 ggcacaccca aagaccccat catgtatttg aatacctcca tgttgcgcaa tatgctcggc
      121 aatttggaca gcggcaccaa aatcaaagtg ggcggcgatt atgcggcttt ggaagcggct
      181 gccaccaaag tgttggcgac aggcacacag ttgaagcaac aagccgacag cttggaagcc
      241 tatttcaaat cgaaaaaata tttggacgat ggctatgcca aatccaaggc ggaaaatgcc
      301 gcgtttgaaa agcaatggga acaattcaat gccgattatg aggtgttcaa taccgagctg
      361 agcaaagtgg aaaaagtcca acgcttggcc agcatcgaaa gcttcaccaa agcaggcaaa
      421 ttgcgcgaag ccgcccaaga aaaagccttg atgtcggcca atgaggtatt ggaaaccctc
      481 agcagcgtag aagacctcaa taatgctgaa aaaatggcca cagccgatgc ggaaatgcaa
      541 aaattggaag cggctttgac cgagttgaac gatgccaatg gcaaaaccaa agacagcgat
      601 tcgtataaat tcgagggcac attcgattat ttgacgtcat ttaccggcta ttggcgcgaa
      661 atgaaggccg aaaaagaccc ttcgcgttac gagcgcatgg tagacagcta taacagcgca
      721 gtgcgttaag ccaaatcaaa atacagtatg aaagcggcct gtaaatccat ggaacatatt
      781 tacaggccgc ttttcatgag acttagagat tgaaattaat gggttaaaac cactattttg
      841 aagcggggta tcatgtgttt cacaatgtta tgaatgacat tgtttacaat taattcacct
      901 ttctctgagc tgctggagtg agaggtgtgt aaacaccctg tggcagggat gacaccttcc
      961 aaaggcggat aactgtgaaa atgcagtaac tcttcgatgt gatcgttggg tattttcgtg
     1021 ctgtccacca aattagggtg aaaatacatc atcaatgagg tttcagaaat ggctgcatgt
     1081 tctaaagccc aacctggaaa aggcacttga ttaaagactt gatcgacaat gtgagaatct
     1141 aaattgtccc accagttgac gatgaagatt tgactgttga attccgcttc tttactggct
     1201 aaatcagccc cttctaacac aaatgcttca ttttcataat ggcaattaaa aatcacaatg
     1261 tgtttccagc catctcgagc aaactcattc acgatgtctt tggttagctg aatgagtgta
     1321 ttgccattta aatcaatcgt gcctggaaaa aatggaccac caccacttaa gggtaatgat
     1381 ttgtagccat agtttaaagt aggggctacc acgccattga tttgtttggc taaagccaag
     1441 ctaaacgctt tagccaacat tgcatcgact ccaagtggca gatgcggacc gtgttgctcg
     1501 gtggctccta tggggataat caaaatatgc tggtttttga ttttgtcttg gtagtcatac
     1561 catgatattt catttaataa ataagacatg tggttatcct ccatttaaaa ttatactttg
     1621 gttaatgcat catttttctg gttgcggctt tgaacgaccg ttttaattcg atacaaaata
     1681 atgactacgg caaaactgac tacgctgaga aacaattgtg agcgtgtatc tgggttatat
     1741 gccatggtta agataatcag gcacaaagac acaatggtga agatggttaa ataagggaag
     1801 caccacatgc gaatttttaa tttttcagga cactcgcgct catatttttt acgcaatatt
     1861 aattgagaaa ttgcgattaa gaggtataca aaaatggcaa tggcaccaga tgagttcagc
     1921 aaaaactgga atacggtgtc tggtgaaaca aaagccatgg caacagatac aaagccaaat
     1981 acagtgcagg ttaaaatggc gcgaacaggt acaccattgt tggtgagctt tttaatggct
     2041 tgaggggcat cttgtttatc ggcaagagcg aataacatgc gagaagctgt gtaaattcct
     2101 gagtttaagc aagacaatac ggcggtaaca atgaccaaat tcataatttt gtcagcatag
     2161 ggaataccga ttgttttcag cgcactgaca taagggttta ccaaagcttg gtggtcggtc
     2221 caaggcacaa tcataaccac taaaaacacc gagcctacat aaaaagtcat gatgcgtaca
     2281 atcactgagt tgactgcacg taccacggcg cgttctgatt caactgactc agctgccgct
     2341 atcgtgacga cttcagagcc gacaaaagag aagatgatgg tggccacagc caaaaatgcc
     2401 gctgagtaac ctaaaggggc aaagccgcca tgggcagtta ggttagagaa atccattttg
     2461 gtattgggaa gcaggcccag tacaaaagca ctggcacaaa ttaagaacaa tacaatggca
     2521 acgattttga tgagtgagaa ccagtattca aattcgccat aagcacgaac agaaaacagg
     2581 ttggtgatgg tcagcaaaac cataatggat aaattgagta cccacaatga cgtatcggga
     2641 aacaaccagt cttttaaaat agcggcacct gcaatggctt caaccgcaat gacaatcacc
     2701 caaaaatacc agtatagcca gccaacagag aaaccggccc agttgccaag ggcttctcgt
     2761 gcatagtcag aaaaagaacc cagtgatggc tttgcaacca ccatttcacc taacatgcgc
     2821 atgaccataa tgaccaacag tcctgcgatt aaggcagtga caacggcggc agggccagtc
     2881 agcttaatca gagccccact gccgacaaac aggcccgcac caatcgatcc accaaaggca
     2941 atcatggtga ggtgtcttaa ttgcagactt ttctttagtt cttgtgacat atcattcctc
     3001 caactttctc gtgtagagtg ctgcatggct tgcaagcgac aaatatgcat taatgcgagc
     3061 aatagtttat ttttttcatg ggtttaggat gcccttgttt tttcatcgta ggtagtgtgt
     3121 ctgttttaaa acatggccaa tgcgaagtat attggtctga atgcgactga acttttttac
     3181 ccatcttgat aatggataaa tgattgaagt aagaaaatga tgtcgaaaat ggctgtgagt
     3241 ttgctatggt tttatatgga ctctgatttt tgcatcatta ataattaatt ggaatttttg
     3301 aggaggagtt tattgcattg caataaaaat cctttaaatt tcaattgctt tgattatttt
     3361 ttgctaattg attttcattt tgaaaattca gcattttttc agaaggtatt tacttcggtt
     3421 ttatcagttg aatgagggga ctcgtaattt cacctaggcc atcatgtatc agtcacccag
     3481 aatgagatgt agatggagtg tgtgtatcgc tttgtgtgag tgatcaatca caaaaattca
     3541 aatgagaaaa tcggaggcag ttacttgagc tgaaaactgc gaaaataacg tggtgataag
     3601 tcacaaagaa tgatgaaaaa tgcagaagca tggcttaaat gagtacagca aaatcccact
     3661 atttttcgta ccaaataccg caaaatttgc tattttagtc taaaaaacct agcaatacgc
     3721 acgacttgtt tcacatgaaa cccaagcggc ctgtaagcca tttacaggcc gcttattttt
     3781 ttgcaaagca cactccaatt ttgttggtgt aaatgcttga tgccgttgtg caatcaagcc
     3841 acactgtcca tgaagattgg ccacaagccg atactgaggc ctgcgaacaa acgcgaccgc
     3901 caccgcacca taccgacggt gcgaaccata aaaacaaaga caacgcgtgt tacccacatg
     3961 tagtagagga gaatcacccc atgcaatcca cacctgatac tcaggcgatt aacaacgcct
     4021 tgtcgctcga cgataaattc actcaagacc acggccgcat ctacatcagc ggcatccaag
     4081 cattggtgcg tttgcccatg ttgcaaaagc agcgcgatgc gcgcgcaggg ctcaataccg
     4141 caggctatgt gtcgggctac cgcggctcgc ctttgggcgg cgtcgatttg gccttgtggc
     4201 aagcgaaaga acatctgcaa aaaaacgaca tcgtgtttca gcccggcctg aatgaagact
     4261 tggccgccac cgccgtgtgg ggcactcagc aaaccaattt gtacgaaacc gccacccgcg
     4321 atggtgtgtt tggcatgtgg tatggcaaag gcccaggcgt agaccgctcc atggatgtgt
     4381 tgaaacacgc caatgcagca ggcacatcgc cgcatggcgg ggtgttggta ttggcgggcg
     4441 atgaccatgc agccaaatcg tcatcggtgg cgcaccagtc tgagcatgtg ttgctggcag
     4501 cgggcatacc cgtgttatac ccgtcgaatg tgcaagagta tttggatttt ggtttgcatg
     4561 gctgggcgat gagccgctac tcgggcttgt gggtggcgat gaaatgcgtc accgatgtgg
     4621 tggagtcttc ggcttcggta gaggtaaatc ctgagcgtgt ggacatcaaa atccccacag
     4681 attttcaaat gcctgccgat ggattgaaca tccgctggcc agacacgccg ttggcacaag
     4741 agcagcgttt gtacgattac aaatggtatg cagcgctgga atacatccgc ttaaacaagt
     4801 taaaccgcat tgaaatcgat gcgccgcaag cgcgttttgg catcatgacc gcgggcaaag
     4861 cctatttgga tgtgcgccaa gccttggcag atttgggctt gagcgatgaa gtgtgcgaaa
     4921 aaatcggcat acgtttgttc aaagtgggct gtgtgtggcc attggatgcg cgcgatgcgc
     4981 gcgaatttgc cacgggttta gacgagattt tggtggtgga agaaaagcgc caaattctgg
     5041 aatacgcgtt aaaagaagaa ttgtacaact ggcgtgaaga cgtgcgccca agcgtgtacg
     5101 gtaagtttga cgagcgcgac cacgctggtg gcgaatggtc gcagccgcgt ggccaatggc
     5161 tgttgcctgc acgttatgaa ttgtcgcctg ccatcattgc caaagccatt gccagccgct
     5221 tgctgcgcat ggatttgcct gaagacattg ttgccaaaat caatgcgcgt ttggatgtga
     5281 tacgcgccaa agagcaagag gccaaagtgc ctcgcattac ggtggaacgc aagccttggt
     5341 attgttcagg ctgcccacac aacacctcga ccaaagtgcc agaaggctcg cgtgcgatgg
     5401 cgggcatagg ctgccattac atggtgatgt ggatggacag aaacaccgaa accttcaccc
     5461 aaatgggcgg cgaaggcgtg acgtggttgg ggcaaatgca ttttacccgc gacaaacatg
     5521 tgtttgtgaa tttgggcgac ggcacgtatt ttcactcagg cttgttagcg atacgtgcat
     5581 ccatcgccgc caaggccaac atcacttaca aaattctgta taacgatgcg gtggcgatga
     5641 cgggcggtca gcctgtggat ggcgtgctca ccgtgccgca aatcgcgcag caagtgttgg
     5701 cagaaggcgc ggccaaactc atcatcgtca gcgatgaacc ggaaaaatac gccgaacgca
     5761 tgggcatacc cgacagcgtc accattcacc accgcgatga atttgacggc ttgcaaaagc
     5821 aaatgcgcga agtggaaggc gtttccatca tcatttacga ccaaacctgc gccaccgaaa
     5881 aacgtcgccg ccgcaaacgc ggcacttatc cagaccccgc caaacgcgtg ttcatcaaca
     5941 ccgatgtgtg cgaagcctgt ggcgattgct cggtgcaatc gaattgcctg tccatcgaac
     6001 ctaagcccac cagccacggc aacaagcgcc aaatcaacca atcaacctgt aataaagact
     6061 tttcctgtgt gaaaggcttt tgccccagct ttgtcacctt aaacggagcg caattgcgca
     6121 agcccaaagt gagcgagcaa aagtttgatg tggcggcctt gcccgagcct gtcttgccca
     6181 aactcgatca tgcttacggc attgtgatta cgggtgtggg cggcacgggt gtgattaccg
     6241 tgggtgcttt gctcggcatg gcggcgcatt tggagcaaaa aggcgtgac
//
LOCUS       DAOMVK010000075         6279 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_3343_length_6279_cov_3.141710, whole genome shotgun sequence.
ACCESSION   DAOMVK010000075 DAOMVK010000000
VERSION     DAOMVK010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 6279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6279
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3343_length_6279_cov_3.141710"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PK856_03900"
     CDS             complement(<1..196)
                     /locus_tag="PK856_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01551.1"
                     /translation="MWKMASIKYLNEDTKAACKHVLNIYRPLICVLNQIKTLGGNAYS
                     NANCPSLAVIMMVDSGAYTPA"
     gene            complement(278..547)
                     /locus_tag="PK856_03905"
     CDS             complement(278..547)
                     /locus_tag="PK856_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01552.1"
                     /translation="MKKSVLMGAITVLGITSAMAGSIANFTNASEQVNPFASQNAHIS
                     KQAVTVAKQNHGKINPKETNPLYQYTGVSTVSDYQSHESAFAVNH"
     gene            complement(772..3582)
                     /locus_tag="PK856_03910"
     CDS             complement(772..3582)
                     /locus_tag="PK856_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01553.1"
                     /translation="MDAEQKLIIGLGDLFKTSAHVDALMPAAIGSNHAAVRICSSKSQ
                     ENLTDSIFLGQPHCIDMRRQLLKQLLPFLNRSDAFDQKTPFSGFDVSRRFARLLFSQH
                     QDWVWSSIVSSLREANVEAHNIVILIDMADSFASAVWIDCLFGLKARYPQASFYPVFL
                     LPQMENLAVEEAAVIGAAVTELSQLSQGRWTPTDINTGEDYPCGEPLWAVAYLQQCPQ
                     VQNDATFLQLVDAVLTLSNVGHNDYGTLRQTLTQEYQKILKPSNAKSAKILPRFASML
                     QASLDFDVEALEGLLIEQCESKLLASLTNTAAPEQTQNLDIATMTALGCVLTHTQLTL
                     DEDSCGEHIGVWSELDMDWQQHSATLLQQNLSGNWLEQAEHIASQLRAVYDKQFRKMG
                     AEKYYEASEMRLNQMAQNHVAYVETQLWHAWQQGRSSLSDLQAAVEGIIIHYVQCHQD
                     FLAHQENNIDAIKANATWWENLSGQWQSADKKERALLTKEYSLETVAEHMSRLFIERC
                     QYKAHVFAEKMILAMQPYLLDLQQDLHNASEAWLVQATHIQKTLNQTIESQQANWLKQ
                     VIQAMHCRYLLQPNKASLQSLVNHTRNAARLVVPELNADCVNKLAKKNGFAALIEYIQ
                     AGSWKESVRVAATKASKSILANEPALLAEILEQRITELDQQQVQYLGKYALSNLSKQF
                     RKLIQPLGKVLTTQYAVNESACLACAEHIQQHELVTSWLAHSAQQSLPKAKVIALNGV
                     NGAQFIYASTCYLDEWVDLPEFLQAYRQFNHSDEALLHLHIDGSNAAISVMQKNSINR
                     NRELIRQHLLLAHASGRLVQEAGHYQLQVTEKSKALDFNTEQLADVVDYIGLDDVQKL
                     VDVHEHMQNEKVFIQEAEQIGNHLEEVLAQIKRESLPAGVDLEDADWADAGRYVVWSR
                     AVQELRKQWLKEKGSRKSQKVA"
     gene            4064..5752
                     /locus_tag="PK856_03915"
     CDS             4064..5752
                     /locus_tag="PK856_03915"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRL01554.1"
                     /translation="MQRKNPHEMLHSLKQQRAALAISVFTVVTVLLLINNYQHLPTAN
                     QSGRYAQLLGIALALFVGVIAYFNITWSKRKQAQLIDTQQQTHTILNTVGSGLFLINQ
                     DLKISKEYSKQLETILGQHHLAEQNLLQILSQLVTEHELMVIQGFLKQLFDVKVNERL
                     LQELNPLNQIDVHFMGTHGKRINRCLDFQFTRIHEHKNIVSVLVNVSDITRSVQQQQQ
                     TIFRSDLQANLLLAIMHANHDAVLHFNKQSKLQLETIHEILAQEGGSTKSHKLKIEPL
                     TQYIHQLRHSANQLNLQILVYLSDEFEQHLLELKQQNYLTHDDFLALDITLDQMDHVL
                     QTISQVHAHLQPIKPVTEASPVHLTSPETTLQRWQNDVQQLAKRHQKCIHLICTNASF
                     QNLPLHLVDKIQELTWHLINNAVVHGIEPAEERETKAKTEHGQVTVSLLDMGSEYRLS
                     VEDDGRGLDFEAMRAQAAELGWCQNNTDLPINQTQMLRFLFQAEFTTLNQMNSDAGDG
                     MGLYWVQTWIEEMGGRIDVVTQPHQFTRFIISIPKPSPGQAQLTAHEQNTHTPT"
     gene            complement(6068..>6279)
                     /locus_tag="PK856_03920"
     CDS             complement(6068..>6279)
                     /locus_tag="PK856_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003769197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HRL01555.1"
                     /translation="GELRVWRENRDELLAIKAGEFAYEDLLNRAQTLMQTVEQAFLHS
                     HLPDAPDLQQLCRVLVTLRQAFYRQ"
BASE COUNT         1759 a         1534 c         1295 g         1691 t
ORIGIN      
        1 tggcgggcgt gtacgcacca gagtccacca tcatgattac cgccaaagac ggacagttgg
       61 catttgaata agcattacca ccaagtgttt taatctggtt taaaacacaa ataagcggcc
      121 tgtaaatgtt taaaacatgt ttacaggccg cttttgtatc ttcattcaaa tactttattg
      181 atgccatttt ccacacccaa aaaaagcagc cctaaggctg caaaaaaggc aaaagtgaaa
      241 caagaaagcc ctgtttaaac aaggctgtca gggcttgtta gtggtttact gcaaatgccg
      301 attcgtggct ttggtaatca gaaactgtgc tgacacctgt gtattggtac aaagggttgg
      361 tttctttggg attgattttg ccgtggtttt gtttggccac ggtcacggct tgtttggaaa
      421 tgtgtgcatt ttgcgaggca aatggattca cttgttcgct ggcattggta aagttggcaa
      481 tcgagcctgc catcgcagaa gtaatgccca atacggttat cgcgcccatc aatactgatt
      541 ttttcatggt cttgccctta acttgtgtta ttgatttgat ggattcattc tattaccaga
      601 cgaatactta ataaatagac ataaaattaa cacagcgtta accataaaga aaataattac
      661 tgacagtcca aactgagcaa cacacttaga ccgatttgtg tccaacaaaa aaaacggcac
      721 caccgcaatg cggtgatgcc ctcaaaagct taaggtaagc aatgaaactg tttacgcgac
      781 tttttgtgac ttgcgactgc ctttttcttt gagccactgt ttgcgcaatt cttgtactgc
      841 ccgactccaa accacataac gccctgcatc agcccaatcg gcgtcttcta aatccacccc
      901 tgcgggcaag ctttcgcgct taatttgtgc caatacttcc tccaaatggt tccctatttg
      961 ttctgcctct tgtatgaaga ctttttcgtt ttgcatgtgc tcatgcacat caaccaattt
     1021 ttgtacatcg tccaaaccaa tgtaatccac cacatcggcc aattgctcgg tattgaaatc
     1081 caaggctttg gatttctcgg tcacttgcaa ttggtaatgc cctgcttctt gtaccaagcg
     1141 gcctgaagca tgggccaata acaagtgttg acgtatcaat tcacgattgc gattgatgct
     1201 gtttttttgc atcacgctga tggctgcatt gctgccatca atgtgcaaat gcagcagcgc
     1261 ttcatcgctg tgattgaatt gacggtaggc ctgtaaaaat tctggcaaat ccacccattc
     1321 atccaaataa caggtgctgg catagatgaa ttgcgcacca ttcacgccat tcaaggcaat
     1381 gaccttggct ttgggcaggc tttgctgtgc gctgtgagcc agccatgagg tcaccaattc
     1441 atgttgctgt atgtgctccg cacaggccaa acaagcactt tcgttgacgg catattgcgt
     1501 ggtcaagacc ttgcccaagg gctgtatcaa tttgcgaaat tgcttgctta aattgctcaa
     1561 ggcatattta cccaaatact gaacttgctg ttggtctagc tcagtaatgc gctgttccaa
     1621 gatttcagcc aacaaggcag gctcgttggc caaaatgctt ttcgaggctt ttgtcgcagc
     1681 cactcgcacc gattctttcc aactaccggc ctgtatgtat tctatcaagg cggcaaaacc
     1741 atttttttta gccagcttgt tgacacagtc ggcattcagc tcgggcacca ccagtcgcgc
     1801 cgcattgcgc gtgtggttca ccaaagactg caaactggcc ttgttgggtt gtaacaaata
     1861 gcgacaatgc atggcctgta tcacctgttt caaccaattg gcttgctggc tttctatggt
     1921 ttgattcagt gttttttgga tgtgagtggc ctgcaccaac caagcttccg aggcattgtg
     1981 caaatcttgc tgcaaatcca ataaataagg ctgcatggcc aaaatcattt tctcagcgaa
     2041 cacatgggcc ttgtattgac agcgttcaat aaacaagcgg ctcatgtgtt ccgccacggt
     2101 ttctaggctg tactcttttg taagtaaggc ccgttccttt ttatcggcgc tttgccactg
     2161 ccctgacaga ttttcccacc aagtggcatt ggctttgatg gcatcaatat tgttttcttg
     2221 atgcgctaaa aaatcttggt ggcattgcac ataatggatg atgatgccct caacagcggc
     2281 ctgtaaatca cttaagctgc ttctgccctg ttgccaagca tgccacaatt gggtctcaac
     2341 ataggccaca tggttttgcg ccatttgatt caaacgcatc tcactggctt catagtattt
     2401 ttcagccccc attttgcgaa actgcttgtc gtaaaccgca cgcaattggc tggcgatgtg
     2461 ctcggcttgt tctaaccaat tgccacttag attttgttgc agcaaagtcg cactgtgctg
     2521 ttgccaatcc atgtccagct cgctccacac accaatgtgc tcaccacaac tgtcctcatc
     2581 caaagtcagt tgtgtgtgcg tcaacacaca acccaatgcg gtcatggttg ctatgtcgag
     2641 gttttgggtt tgttctggag cggctgtatt ggttaaggaa gccaacaatt tcgattcaca
     2701 ctgctcaatc aacaagcctt ccaaggcctc cacatcaaaa tcaagcgaag cctgtagcat
     2761 ggaagcaaag cgcggcagaa ttttggcgct tttggcatta ctgggtttta agattttttg
     2821 gtattcttgg gtaagcgttt ggcgcaaagt gccataatca ttgtgcccca cattggacag
     2881 cgtcaacaca gcatccacca attgtaagaa tgtggcgtcg ttttgtactt gtgggcattg
     2941 ctgcaaataa gcgacagccc acaaaggctc tccacaagga taatcttcac ccgtattgat
     3001 gtccgtaggc gtccaccgac cttggctgag ctggcttaat tcagtgacgg ccgccccaat
     3061 gactgcagcc tcttctactg ccaggttttc catttgcggc aacaaaaaca caggataaaa
     3121 actcgcttgg ggatagcggg ctttcaagcc aaacaagcaa tctatccaaa ccgcactggc
     3181 aaatgaatca gccatgtcaa tcaagatgac tatattgtgt gcttccacat tggcttcgcg
     3241 caaactgctg actatgctgc tccacaccca atcttggtgt tgcgaaaaca acaatcgcgc
     3301 aaatctgcgc gaaacatcaa agcctgaaaa aggggtcttc tgatcaaacg catcactgcg
     3361 atttaaaaag ggcagcaatt gttttaacaa ctggcggcgc atgtctatgc agtgcggttg
     3421 gcctaaaaat atactgtcgg tcaaattctc ttgtgatttg ctgctgcaaa ttctgacagc
     3481 ggcgtggtta cttcctatgg cagcaggcat caacgcgtca acatgagcag atgtcttgaa
     3541 caaatcaccc aagcctatga ttaatttttg ctcagcatcc atgagttttc cccgcatcag
     3601 tagctgaaat gattcagcct accctttctt atttgttttg gaagtaccag ttttatatag
     3661 aatgaatgta ttggtttcac catcactgcc atgaaaataa attaaagcaa aaacctttaa
     3721 agtatcaata cagttgacag taagttgcat taatcgcaat ctactgcaat caatttggca
     3781 actccaattg atttttaaaa tcaaaatcca cacttcaata aataacaatt ctggccaact
     3841 gatattgcga atatgacaag cctatactaa gatttagcca caaaagaaat caaattttta
     3901 tttgaatgat tttagaataa acacacttta caattccgca ccatgctcta aataccgttt
     3961 aaattcagtc ggtttttaat tattaaagca cttaaacttt attttcatca ttaataatct
     4021 tgatgcaatt ggtatttata tcgcgcatca cagaggtgtt gctatgcagc gaaaaaaccc
     4081 acatgaaatg ttgcattcgt taaagcagca acgcgctgcc ttggccattt cggtgttcac
     4141 tgtcgtaacc gttttgttac tgatcaacaa ctaccaacac ctgccaactg ccaatcaatc
     4201 gggacgttat gctcaattat tgggcatcgc tttggcattg ttcgttggtg tcatcgccta
     4261 tttcaacatc acttggtcta aacgcaagca agcacaactg attgatacgc aacaacaaac
     4321 ccataccatt ttgaatacgg tgggaagcgg cttgttttta atcaatcagg atttgaaaat
     4381 cagcaaagaa tactccaaac aattggaaac catcttgggc caacaccatt tggcagaaca
     4441 aaatttgttg caaattttgt cacaactggt gacagagcat gaactcatgg tgattcaagg
     4501 ctttttaaag caattatttg acgtaaaagt caatgaaagg ctgctacagg aactgaaccc
     4561 actgaaccaa attgatgtgc acttcatggg cactcatggt aaacgcatca acagatgttt
     4621 ggattttcaa ttcacccgca ttcatgaaca caagaacatt gtttcggtat tggtgaatgt
     4681 cagcgacatt acccgctctg tacaacaaca gcaacagacc attttccgct cagatttaca
     4741 ggccaatttg ttgttggcca tcatgcatgc caaccatgac gcagtgctgc actttaacaa
     4801 acaatccaaa ctgcaattgg aaaccataca cgagattctg gcgcaagaag gcggctctac
     4861 caaatcgcac aaacttaaaa tcgagcctct tacccaatac atacaccaac tcagacacag
     4921 tgccaatcaa ttgaatttgc aaatactggt gtatttaagt gatgaatttg agcagcatct
     4981 tttggaatta aaacagcaaa actacctgac ccatgacgac tttctggcgt tggacatcac
     5041 cctagaccaa atggaccatg tgttacaaac gattagccaa gtccacgcac atttacaacc
     5101 cattaaacct gtcactgaag cgtctccagt acacctaact tcgccagaaa ccaccttaca
     5161 gcgttggcaa aacgatgtgc agcaattggc caaacgccat caaaaatgca tccatttaat
     5221 ctgcaccaat gcctcgtttc aaaatctgcc tctgcatctg gtggataaaa tccaagaact
     5281 cacctggcat ttgatcaaca atgccgtggt tcatggcata gagcccgcag aggagcgaga
     5341 gaccaaagcc aaaaccgaac atgggcaagt gactgtgtca ctgctggaca tgggttctga
     5401 ataccgattg agcgtagaag atgacggcag aggcttagac tttgaggcca tgcgagcgca
     5461 agcggctgag ctgggttggt gtcaaaacaa taccgatctt cccatcaacc aaacccaaat
     5521 gttgaggttc ttgtttcagg ccgaattcac cactttaaac caaatgaaca gtgatgcagg
     5581 agacggcatg gggctgtatt gggtacagac ttggatagaa gaaatggggg ggcgcatcga
     5641 tgtggtcacc cagccgcacc agtttacccg cttcatcatc agcattccca aaccctctcc
     5701 tggccaagca cagcttacag cccatgagca aaacacacac acaccaacgt gaccgaggaa
     5761 ggagacgatg gagaggatta cgtggtttag cccaagcatc tggtcacaga gtttctagca
     5821 ccttttcctt gttgggctca agaaaaacca aagcggggca cacaatccat tcaatattga
     5881 atcaaggcaa aagcgagcgg taagtacaaa acacatgagg gcatagccag cacgcccccc
     5941 actaacaaaa aacaaatgcc ctcattctga acttagcacc ccagtttcac acgcccacta
     6001 cacacacttg ggcaatcggc ctgtaaacac atcaaatggt ttacaggccg ctttttatgc
     6061 taaagcatca ttgccgataa aatgcttgcc taagcgtcac caatacccga cagagttgtt
     6121 gcaaatcggg cgcatcaggc agatggctgt gcaagaatgc ctgctccacg gtttgcatca
     6181 aagtctgcgc tctattgagc aaatcttcat aagcaaattc accggcttta attgccaaca
     6241 actcatcacg gttttcccgc cacacccgca gctcgccgt
//
LOCUS       DAOMVK010000076         6228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_3383_length_6228_cov_3.436903, whole genome shotgun sequence.
ACCESSION   DAOMVK010000076 DAOMVK010000000
VERSION     DAOMVK010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 6228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6228
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3383_length_6228_cov_3.436903"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /gene="rsmB"
                     /locus_tag="PK856_03925"
     CDS             <1..826
                     /gene="rsmB"
                     /locus_tag="PK856_03925"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HRL01556.1"
                     /translation="LPQWWIDKLKNDYPKYWHNIVAASQLPPPLTLRVNRRHMTGEAY
                     LALLAEQEIEASLLGEQAVRLKQATSVVNLPQFSEGVVSVQDWGAQQAALLLNPQAGE
                     RVLDACAAPGGKTGHILELADVDLLALDVDAKRLRRVAENVERLGLQARLQAADASKL
                     DDWYDGVQFDAVLADVPCTASGVVKRHPDIKWLRRKTDAQSVALQQHQMLDVLWSTVK
                     VGGRMLLATCSIFPEENHQQLQAMLSRHADARLRLEEQLLPNDAQDGFYYALIDKV"
     gene            792..1379
                     /locus_tag="PK856_03930"
     CDS             792..1379
                     /locus_tag="PK856_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HRL01557.1"
                     /translation="MAFITRLLIKFSLLAVCLSWACATQAESMRATRAEAVVGSQGQL
                     NVSSRFNTTLPSALQDALKQGVTLNFELNYQLVAPKLLAYNVRFNPLVDTVKPVIYKL
                     SYHPLTDKYRVTVGTFSTEYSQLDTALRAVGSIANWQVHPKSTFGRNDAGQIQANVRL
                     NLSSNQLPKPFQVNTLTSKNWNLDSGWVRLRARAG"
     gene            1382..3499
                     /locus_tag="PK856_03935"
     CDS             1382..3499
                     /locus_tag="PK856_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HRL01558.1"
                     /translation="MRIFVLVMAIVTMLLLYMLTIATGHASILSDYFWWIFGFNAIAL
                     VIQLFIVGKQLWQLWRDGRKRVFGARIAKRLALMFVLVTVLPGLFLFGVSAQFIRHSI
                     NSWFGNETEVALERSLNLSKVALEGSVEDALRDVVPLQIEMARALTFQDSVSPLLQQR
                     LRQPEGYRQLAMWDMHKQRWLAQVGGGQQFDWPQLSRGDVRKLQQQGAIGGLESIHET
                     LYIRGWVAIPNVDNADIILFFRKPVPKIIAQDAILIEAARGKYADLSYAKRGLQTFFL
                     MTLLMATLLAVSLALAVALYFSRRFVAPLSALADGTRAVAQGDFSQKSPVLRADEMGM
                     LSSLFNRMTEQLDVAQQLAERNRQQQEAARAYLEQVLASLTTGVISLNAQGVVRSYND
                     SAEHILGMDLAMTEHHPLQDWGQISSQWAVLGQIVVQVLAKVDDVTPVQMDYAGQDKA
                     QILLIKATRITYEQMSHTVIVFDDVTDLVHAQKEAAWGEVAKRLAHEIRNPLTPIQLS
                     AERLAWKLQDKLAEDDVRILIRSTDTIVKQVAALKDMVEAFRNYARSPALRPVEQDLR
                     QVVGEVLVLYESGNCQFQSDLGKMPLKVRIDTALMRQVLHNLFKNAAEATEIAEQPLV
                     EVKAGIEGDSVSLCIANNGKSFSPSILATAFEPYITDKKNGTGLGLSVVKKIIDDHQG
                     KITIANQDAGGAVIRIILPHVEE"
     gene            3499..4776
                     /locus_tag="PK856_03940"
     CDS             3499..4776
                     /locus_tag="PK856_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRL01559.1"
                     /translation="MRSTDILIVDDEVGIRDLLSEILQDEGYTVTEAEDAESARQLRN
                     QTRPAMVLLDIWMPDCDGITLLKEWAQSGQLNMPVVMMSGHASIDTAVEATKIGALDF
                     LEKPIALQKLLTTVQRALKYGQMQQASTLTLEKLGNSAAMSGLSAQLVKIQKHKYPVL
                     LTGEKGSPFELVARTLAGQGAAWVTPAKPEQLLDAGVELLQKAQQGVLYLGDVSQLSI
                     TAQRAVVDLIQKADQYPARIVAYCSRPLEEMLTQSEYESTLINAFANSMVAVPPLREQ
                     ADDLVSVITYLLAELIENRQIPVVKLTVQAQNALRQYDWPGNLEQLKSVLKSLALNAD
                     HGTVDAPQVVALLSQFKQPQSNHVSGGFNFDLPLRELREELEKRYFEYHITQENGNMS
                     RVAQRVGLERTHLYRKLKQLGVQFSKRQAEKAD"
     gene            4816..5379
                     /gene="rsmD"
                     /locus_tag="PK856_03945"
     CDS             4816..5379
                     /gene="rsmD"
                     /locus_tag="PK856_03945"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HRL01560.1"
                     /translation="MKNKQHKQQIRIIGGTHRRRLVHFDEAEGLRPTPDRVREQVFNW
                     LGQRLSGQKVLDLFGGSGVMAMEAASRGAEKVDVVELNRQTVQKIRAVINELKFDQVT
                     VYQQDATAYLQAASEVYDVIVLDPPYRWEEWYGLWEVLRLHCHAETVLYIEAAQLPQL
                     PGWLQWNKQDSAGQSHYGLALVQDIDL"
     gene            5463..5717
                     /locus_tag="PK856_03950"
     CDS             5463..5717
                     /locus_tag="PK856_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HRL01561.1"
                     /translation="MSLFITDECINCDVCEPECPNDAIYQGDEIYEINPSLCTQCIGH
                     YDEPQCQQVCPVDCILIDEVNPETEDQLQAKYEQLQADKK"
     gene            5817..5900
                     /locus_tag="PK856_03955"
     tRNA            5817..5900
                     /locus_tag="PK856_03955"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5851..5853,aa:Tyr,seq:gta)
     gene            5930..6003
                     /locus_tag="PK856_03960"
     tRNA            5930..6003
                     /locus_tag="PK856_03960"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5962..5964,aa:Gly,seq:tcc)
     gene            6015..6089
                     /locus_tag="PK856_03965"
     tRNA            6015..6089
                     /locus_tag="PK856_03965"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6047..6049,aa:Thr,seq:ggt)
BASE COUNT         1643 a         1200 c         1708 g         1677 t
ORIGIN      
        1 cctgccacag tggtggatag acaagcttaa aaatgactac ccaaaatact ggcacaatat
       61 tgtggcagcc agccaattgc caccaccgtt gacgttgcgc gtgaaccgtc gccacatgac
      121 gggtgaggcg tatttggctt tgttggctga gcaggaaatt gaagccagct tgttgggcga
      181 gcaggcggtg cgtctgaaac aagcaacatc tgttgtaaat ttgccacaat tttctgaagg
      241 tgtggtatcg gtgcaagatt ggggcgcaca acaggcggcc ttgttgctga atccacaagc
      301 aggtgagcgt gtattggacg cttgtgcggc gccagggggc aaaacaggtc acattttgga
      361 attggctgat gtggatttgt tggccttgga tgtggatgcc aaacgcttac ggcgcgtggc
      421 tgaaaacgta gagcgcttgg gtttacaagc gcgtttacag gccgccgatg catcaaaatt
      481 agatgactgg tatgatgggg tacagtttga tgcggttttg gccgatgtgc cttgcacggc
      541 ttctggcgtg gtgaagcgcc accccgacat caaatggctg cgtcgcaaaa ccgatgctca
      601 atccgttgca ttacagcaac accaaatgct ggatgtgttg tggtctaccg tcaaagtggg
      661 tggtcgcatg ttattggcaa cgtgctcgat ctttcctgaa gaaaatcacc aacaattgca
      721 ggctatgctc agtcgccatg ccgatgcgcg tttgcgctta gaagagcaac tcctgccgaa
      781 tgatgcacaa gatggctttt attacgcgct tattgataaa gtttagcctg ttggctgtgt
      841 gcttgagttg ggcttgtgcc actcaggcag agagcatgcg tgcaacacgg gcagaggcag
      901 tggtaggttc gcaagggcag ctcaatgtga gcagtcgctt caataccacg ctgccttctg
      961 ctttacagga cgctttaaag caaggggtca ccctgaattt tgaattgaat tatcaattgg
     1021 tagcaccgaa gttgttggcc tataacgtgc ggttcaatcc tttggtggat acggttaagc
     1081 ctgtcatcta caaactttct tatcacccat taaccgataa ataccgtgtg acagtgggga
     1141 ctttttctac tgaatacagc caattggata cggctttacg ggctgttggc agtattgcaa
     1201 actggcaagt tcatccaaaa tcaacatttg ggcgtaatga tgcgggtcaa attcaagcca
     1261 atgtgcgttt aaatctcagc agtaatcaat tgccaaagcc ttttcaagtc aataccttaa
     1321 cctcaaaaaa ttggaatttg gattctgggt gggtgcgctt acgagcaaga gcggggtagg
     1381 catgcgaata tttgtgttgg tgatggccat tgtcaccatg ttattgctgt acatgctcac
     1441 cattgccacg ggtcacgcat ctatcttgtc tgactatttt tggtggatat tcggcttcaa
     1501 tgcgattgct ttggtaatcc agttgttcat agtgggtaag cagttatggc agttatggcg
     1561 ggatggccgc aaacgggtgt ttggagcgcg gattgcaaaa cggcttgcac tgatgtttgt
     1621 gttggtaacg gtattgcctg gcttgttttt gtttggagtg tcggcgcagt ttattcgcca
     1681 cagcatcaac tcttggtttg gtaatgaaac tgaagtggct ttggagcgta gcttaaacct
     1741 cagtaaggtg gctttagaag gcagcgtgga agatgcactc cgggatgtcg tgcctttgca
     1801 gattgaaatg gctcgtgctt taacatttca agatagtgtt tcccctttat tgcaacagcg
     1861 actgcgtcaa ccagagggct atcggcaatt ggccatgtgg gacatgcaca aacaacgatg
     1921 gttggcacaa gtgggtgggg gacagcagtt tgattggcct caattgagtc gaggggatgt
     1981 gcggaaattg cagcaacaag gtgcgattgg tgggctagag tctattcacg agactttgta
     2041 tattcgtgga tgggtagcga ttccaaatgt tgataatgct gatatcatat tgtttttccg
     2101 caagcccgtg cctaagatta ttgcgcaaga tgccattctg attgaggctg ctcgcggtaa
     2161 gtatgcggat ttaagttatg ccaaacgagg cttgcaaact tttttcttaa tgactttgtt
     2221 gatggcaacg ctgttggcag tgtctttggc cttggctgta gcattgtatt tctctaggcg
     2281 ttttgtcgct cctctgtcgg ctttggctga cggtacacgt gcagtagcac agggggactt
     2341 tagtcagaag agccctgtgt tgcgggctga tgagatgggc atgctcagta gtttgttcaa
     2401 tcgaatgacg gaacaattgg atgtggcaca gcagttggca gaacgtaatc ggcaacagca
     2461 agaggctgct cgtgcatatc tcgagcaagt tttggccagt ttgaccactg gtgtgatttc
     2521 gctcaatgca caaggcgtag tgcgctcgta taatgacagt gcagaacaca ttttgggcat
     2581 ggatttggct atgactgagc accatccatt gcaagactgg gggcaaattt ccagtcagtg
     2641 ggcggtatta ggtcaaattg ttgtacaggt tttggccaaa gtggatgatg tgacgccagt
     2701 gcaaatggat tatgcaggtc aggacaaagc gcaaattttg ttgattaaag caactcgcat
     2761 tacgtatgag caaatgtcgc atacggtgat tgtgtttgat gatgtaacgg atttggttca
     2821 tgcgcagaaa gaggctgctt ggggagaggt tgccaagcgt ttggcacatg agattcgcaa
     2881 tcctttgaca ccaatccaac tgtccgcaga gcggctggca tggaaattgc aggataagct
     2941 cgctgaagat gatgttcgca ttttaatccg ctcaactgat accatagtga agcaggtagc
     3001 agcactgaaa gacatggtag aagcttttag aaactatgcg agatcccctg cgcttagacc
     3061 tgtagagcag gatttgcgac aggttgtggg agaggtattg gtgttgtacg aaagtggaaa
     3121 ctgtcaattt caatctgatt tgggtaagat gcccttaaaa gtgcgcatag atactgcttt
     3181 aatgaggcag gtgttacata acttatttaa aaatgcagct gaagcaacgg agattgctga
     3241 gcaacctttg gtggaagtta aggccggtat agaaggtgat tcagtatctt tgtgtattgc
     3301 aaataatggc aaaagtttta gcccaagcat tttggctact gcgtttgagc catacatcac
     3361 tgataagaaa aacggtacag gtttgggttt gtctgtggtg aagaaaatca ttgatgatca
     3421 tcaaggtaaa atcaccattg ctaatcaaga tgctggtggc gcggtcatac gaatcatttt
     3481 gccccatgtg gaggaataat gcgcagtaca gacatattaa tcgttgatga tgaagttggg
     3541 atacgcgact tattgtccga aatcttgcaa gatgaaggct ataccgtcac tgaggcggag
     3601 gatgctgaat ctgctcgtca actgcgcaat cagaccagac cagcaatggt tttgttggat
     3661 atttggatgc cagattgtga tggaataact ttgttaaaag agtgggcgca gtcggggcag
     3721 ttaaacatgc ctgtggtgat gatgagtggc catgccagca ttgatacagc ggttgaagca
     3781 accaaaatag gggctttgga ttttttagag aagcccattg ctttgcaaaa attgctgaca
     3841 accgtgcagc gggcattgaa atatgggcaa atgcaacaag ccagtacttt gacccttgag
     3901 aaattgggca atagcgcagc catgagcggc ctgagcgcac agttggtgaa aattcaaaag
     3961 cacaaatatc ctgtgctatt aacaggtgaa aagggttctc catttgaatt ggtggcgaga
     4021 accttggcag gacaaggggc tgcgtgggtg acacctgcta agccagagca attgcttgat
     4081 gctggtgttg agttgttgca aaaagcccag caaggtgtgc tttatttggg ggatgtatca
     4141 caattgtcaa ttactgcaca acgggcagtg gtagatttga ttcaaaaggc agaccaatac
     4201 cctgcacgta ttgtggccta ttgcagccga cctttggaag aaatgctgac acaatctgag
     4261 tatgagtcta cattaatcaa tgcctttgca aactcgatgg tggcggtgcc tccgttgcgc
     4321 gagcaggcag atgatttggt gtctgttatc acgtatttgt tggcagagtt gattgaaaac
     4381 aggcaaattc cagtggtgaa gttgacggtt caggctcaga atgcattgcg tcaatatgat
     4441 tggccaggca atttagagca gctgaaaagt gtcttgaaga gtttggcctt gaatgctgat
     4501 catggcacgg tggacgcacc gcaagtggtg gctttgttgt cacaatttaa acagcctcaa
     4561 tcgaatcatg tttctggtgg atttaatttt gatttgccat tgcgcgagtt gcgtgaggaa
     4621 ttggagaaac gctattttga gtaccacatt acgcaagaga acggcaatat gagccgtgtg
     4681 gcgcaaagag tgggtttgga acgaacacat ttgtaccgaa aattgaagca attgggtgtg
     4741 cagttttcca agagacaagc tgaaaaagcc gattaatcac aagctgccta tgggcagctt
     4801 tttagtttga gccatatgaa aaataagcaa cacaaacaac aaatccgcat tattggcgga
     4861 acgcaccggc gtcggcttgt tcattttgat gaggcagaag gcttgcgacc aacgccagat
     4921 agggtgaggg agcaggtttt caattggcta gggcagcgtt tgagtgggca gaaagtactg
     4981 gatttgtttg gaggtagtgg tgtgatggca atggaagctg cctcgcgcgg tgcagaaaaa
     5041 gtggatgtgg tggaattgaa ccgccaaact gtacaaaaaa ttcgtgcagt aatcaatgaa
     5101 ttgaaatttg accaagtcac agtgtatcaa caggatgcta cagcatattt acaggccgca
     5161 tctgaagtct atgatgtgat tgtgctggac cctccctatc gttgggaaga gtggtatggg
     5221 ttgtgggaag tgctgagatt gcattgccat gctgaaactg tgttgtatat tgaggccgca
     5281 cagctaccgc aacttccagg atggttgcaa tggaataagc aagacagtgc ggggcagagt
     5341 cattatggtt tggcattggt acaagatatt gatttataat catgcttagg tatgtaatat
     5401 tgccaatgag gcccgaagct gtaacaatgc tgggtttatt acgctttttg atgggacaac
     5461 aaatgtcttt gtttattacc gatgagtgca ttaactgcga tgtatgtgag ccagaatgcc
     5521 caaatgatgc catttaccaa ggggatgaaa tttatgaaat caaccccagt ctgtgtaccc
     5581 agtgtattgg tcattatgat gagccgcaat gtcagcaagt gtgccctgtt gactgtatct
     5641 taattgacga agtcaatcct gaaacagaag atcaattgca agccaagtac gaacaactgc
     5701 aagctgataa aaaataataa tgataaaaaa caggttgttg aattattttt aacatgcctc
     5761 aggaaaaatg atgttgactg catgggagcg gtgtattaat atgcagcctc tctagtggag
     5821 ggattcccga gcggccaaag gggggagact gtaaatctct tgcgaaagct tcgaaggttc
     5881 gaatccttct ccctccacca caagaatttg tagttgttgg agttgtagcg cgggtgtagc
     5941 tcaatggtag agcagaagcc ttccaagctt acggtgaggg ttcgattccc ttcacccgct
     6001 ccaaaaaaga ttaggcccat gtagctcagg ggtagagcac tcccttggta agggagaggc
     6061 cggcagttcg aatctgccca tgggcaccaa aatcttacaa tactgcaaca ccaatcttaa
     6121 atggttttgt ttatcaatat ttgcaacaaa ctaaactagg acttaggaat agccatggct
     6181 aaagaaaaat tcgagcggag taaaccgcac gtaaacgttg gcaccatc
//
LOCUS       DAOMVK010000077         6168 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_3431_length_6168_cov_3.671683, whole genome shotgun sequence.
ACCESSION   DAOMVK010000077 DAOMVK010000000
VERSION     DAOMVK010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 6168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6168
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3431_length_6168_cov_3.671683"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="PK856_03970"
     CDS             complement(<1..988)
                     /locus_tag="PK856_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HRL01562.1"
                     /translation="MKPHITTRAGNIEVQQDLQAAGISPLLAQLYAARDIESVAETSH
                     DLAHLLHFRDLKHINAAAQQLAAAIDAGKRLLVIADYDADGATACAVAMKGLSLFGAK
                     VDFLVPNRFEHGYGLTPLIVDEAAALGAELIITVDNGIASLAGVAHAQALGIDVLVTD
                     HHLPADTLPACTIVNPNQPECVFGSKNLAGCGVMFYVLLALRAHYKLTGRYDTQAAPN
                     LGVLLDYVALGTVADVVKLDHNNRILVAQGLKRMRAGKAALGIQALFEIAKRDIRKAE
                     PFDLGFAIGPRINAAGRLDDMSIGIRCLLADNELTAQTLAAELDSLNRERRGI"
     gene            complement(985..1926)
                     /locus_tag="PK856_03975"
     CDS             complement(985..1926)
                     /locus_tag="PK856_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01563.1"
                     /translation="MDNWLRFGNITPQPLSRSKLYAQCLSHQSLLLQCKQELGLPASQ
                     PAFFCSPVLQRVDLHSMSISDGSVLGLSKREAVDICRDFNVFVADSGYKFLPYREYLW
                     LVTCPSEPTWFLPPVCDIEGQLKTVPRPQSDDAKVSAQLQTLQTELQMLLAQHPVNQQ
                     RKTTYQAEINGVWFWRDLPSDTPCSADIPLLTDAPLLQGSARHQTDAPYDWAAAQAWL
                     AETGTHEQAHLWLNTLEAPSMFADMWGYQDAFAELNTRFLEPIDAALQKGEIKSLTLI
                     TDGATGLRCQAHKRNRLAFWKRGGEGFAHYLQAASAE"
     gene            2342..3607
                     /locus_tag="PK856_03980"
     CDS             2342..3607
                     /locus_tag="PK856_03980"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRL01564.1"
                     /translation="MRWTAVPIAMALFGSVTAFAVNDAKETHKPEFTKVVERLAKPKL
                     QQQPLVESYWREEQVAEGENFNSFLMRLGISDTEVHNLLAQKSFSPDLVKLRANQMVS
                     IQADNKGGLSAIQFFSDDDDGEKILLALEKVNGNWQASANEVETVTMPTLRAVNVVTS
                     ARGALAQARVPVEIREAISEIFSDKFALDDLKEGDTVRVLYESHNFRGQEVSTGNIMA
                     LEIGKAGRLYQAYYFDHGDNTGAYYDHRGKALSKGEFAKIPVNATRVSSPYGTRYHPI
                     LKTYRMHSGIDYAAPTGTPIMAPADGVVSFAGVKGGYGNAIMLNHRKGMETLYGHMSA
                     FVSGVNAGKSVKAGDVIGFVGSTGRSTGPHLHYEVRINGQIVDPSTVALPARTLSNTE
                     LAAFKGRVKSSDQKIASIRGLPVMVAQLD"
     gene            3786..4475
                     /locus_tag="PK856_03985"
     CDS             3786..4475
                     /locus_tag="PK856_03985"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRL01565.1"
                     /translation="MLKSSMTGSVAALALMLPLFALAAPKPVSALQRNQFILQENQSH
                     KPSLFLNKADAQQDSEVQIQKTVNTRSARGLYVNHTPATSEYGNRLHPVSKRYKLHQG
                     DDYAAPAGTPIMAPSAGIITYMGRKNGYGNTVMLRHSSGQETLYAHMSRFNKGLKVGD
                     VVQNGDKIGYVGSTGISTGPHLHFEVWVDGKRVRPSSVKLPSKALNASEAELLRTYLK
                     PTGKPAKTIQL"
     gene            4691..5311
                     /locus_tag="PK856_03990"
     CDS             4691..5311
                     /locus_tag="PK856_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01566.1"
                     /translation="MNAMKTTLMGLTAAAVLGVSTLAVAETKPTPQARPQLTEQQKAQ
                     MQERHFERRYGDLKLSKSQQTKIKAIEEQYRPQARAQISDAERQAFAANADKLRQARL
                     NVVQGKTFNEAEAQQVFAQEQAVRNEMQQKHQQQRAQMELTQLRKEHAIFQVLDKKQQ
                     QQYLERAKQPRPMMARDGGKGMKGKPHHRGHGPRPAVAPQAATPAK"
     gene            5510..>6168
                     /locus_tag="PK856_03995"
     CDS             5510..>6168
                     /locus_tag="PK856_03995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HRL01567.1"
                     /translation="MGTPTAYAFVDLETTGGSAQHDRITEIAIIRVEGESVTRYQSLI
                     NPQQHISYFIESITGISNAMVADAPEFAEVADEVWTWLQDCVFVAHNVRFDYGFLKQA
                     FERVGKDFQPEQLCTVKLSRRLYPEHKHHGLDQLIDRHGLRMSQRHRAMADADAIYQF
                     WQIVHQEFAADVLQAACLKVMNKPSLPVYLNPLSIDTLYNGYGVYLIYGDNDLPLYIG
                     K"
BASE COUNT         1580 a         1506 c         1625 g         1457 t
ORIGIN      
        1 cgatgccgcg gcgttcgcgg ttgaggctgt cgagttcggc ggcgagggtt tgcgccgtga
       61 gttcgttgtc ggcaagcaaa cagcgtatgc ctatgctcat gtcgtctaag cgaccagcgg
      121 cattgatgcg ggggccgatg gcaaagccca aatcaaaggg ctcggccttg cggatgtcgc
      181 gtttggcaat ctcaaacaag gcttgtatgc ccaaggcggc tttgcctgca cgcatgcgtt
      241 tgaggccttg tgccaccaaa atgcggttgt tgtggtctag cttcaccacg tcggccaccg
      301 tgcccaaggc cacgtaatcc aataacacgc ccaaattggg cgcggcttgt gtgtcatagc
      361 ggcctgtaag cttgtaatgc gcgcgcaagg ccaacaacac ataaaacatc accccacagc
      421 cggccagatt tttgctgcca aacacacatt caggctgatt cgggttgacg atggtgcaag
      481 cgggtaaggt gtcggcgggc aagtggtgat cggtgaccaa tacgtctatg cccagcgctt
      541 gcgcatgtgc cacgcctgcc aatgaggcaa tgccattgtc tacggtgatg atgagctccg
      601 cgcccaaggc ggcggcttca tcgacgatga gcggggtgag gccgtagccg tgttcaaaac
      661 ggttgggcac caaaaaatcc actttggcac caaacaaact caagcctttc atggccaccg
      721 cgcaagcagt cgcgccatcg gcatcgtagt cggcaatgac gagcaggcgt ttgcctgcgt
      781 ctatcgccgc ggccaattgc tgtgcggcgg cattgatgtg tttgaggtcg cgaaaatgca
      841 gcaagtgcgc caagtcatgg ctggtttcgg caaccgattc gatgtcacgc gcggcataca
      901 attgtgccaa taatgggctg atgcctgcgg cctgtaagtc ttgctgtact tcgatattgc
      961 cagcgcgggt ggtgatgtgt ggtttcattc ggcagaagcg gcctgtaaat aatgggcaaa
     1021 gccttcgccg ccgcgtttcc aaaatgccaa acggttgcgc ttgtgtgctt ggcaacgcaa
     1081 acctgtggca ccatcagtga tgagggtgag gcttttgatt tcgccttttt gcaaggccgc
     1141 atctataggt tctaaaaagc gcgtgttcaa ttcggcaaac gcgtcttgat aaccccacat
     1201 gtcggcaaac atgcttggtg cttctaaggt gttcagccac aaatgcgctt gctcatgcgt
     1261 gcctgtttcg gccaaccatg cttgcgcggc agcccaatcg taaggcgcat cggtttgatg
     1321 gcgcgcgctg ccttgtaata agggcgcatc ggtcagcaaa ggaatgtctg cgctgcaagg
     1381 tgtatcagac ggtaaatcgc gccaaaacca cacgccgttg atttcggctt ggtaagtggt
     1441 tttgcgttgt tggttgacag ggtgttgggc gagcagcatt tgcaactcgg tctgtaaggt
     1501 ttgcaattgg gcgctgactt tggcatcgtc gctttgtggg cgtggcacgg ttttgagttg
     1561 gccttcgatg tcgcacacag gcggcaaaaa ccatgtgggc tcactcgggc aagtcaccaa
     1621 ccacaggtat tcgcggtagg gcaggaattt gtagccgctg tcggccacaa acacattgaa
     1681 atcgcgacaa atgtccacgg cttcgcgttt gctcaagcca agcacactgc catcgctgat
     1741 gctcatgctg tgtaagtcca cccgttgtaa cacagggctg caaaagaaag caggctggct
     1801 tgcaggcaaa cccaattctt gtttgcactg caagagcaaa ctttggtggc ttaaacattg
     1861 ggcatacagt ttgctgcggc tgaggggctg aggcgtaatg ttgccaaaac gcaaccaatt
     1921 gtccattact tctgtatgag ccagtttggg cgtttgacct gtggccgccg tgtgaggcca
     1981 taataatcca ggcagataaa gcgacaggtg catgagcgac catccaaatg aagacaaggc
     2041 cgctattcta acccatcgcc atcacagcgg gtatccatgc ttgtgttgaa acgctgactt
     2101 gaaattgagt aaacacacct catttttagg cttaagtggc ttgattgcac tggattaatt
     2161 ttgttcacaa aattgtaaag tggtggaaaa agattaggtt caggccgata agtggtttta
     2221 taatcgtcac catattaatg aatttgcttt tgcgccgccg aagtttgttt gacggcgcat
     2281 ttgatattga aggataaaca tccgcgtgtg gaatttaaaa cagtcagcaa ccgttaaaaa
     2341 aatgcgttgg accgcagtgc ctattgccat ggcattgttc ggttcggtta cggcgtttgc
     2401 ggtcaacgat gccaaagaaa cccacaaacc cgaattcacc aaagtggtgg agcgtttggc
     2461 caaacccaaa ttacagcaac agcctttggt agaaagctat tggcgcgaag agcaagtggc
     2521 tgaaggagag aatttcaatt ctttcttaat gcgtttgggc atcagcgata ccgaagttca
     2581 caacttattg gcgcaaaaaa gctttagccc cgatttggta aaattgcgcg ccaaccaaat
     2641 ggtcagcata caggccgata acaaaggcgg tttgagcgcg atacagtttt tcagcgacga
     2701 tgatgacggt gaaaaaatcc tcttggcctt ggaaaaagtg aatggcaatt ggcaagcgtc
     2761 tgccaatgaa gtggaaaccg tgaccatgcc gactttgcgg gcagtcaatg tggtgacttc
     2821 tgcgcgtggc gccttggcgc aagcacgtgt gcctgtggaa attcgtgaag ccatcagtga
     2881 gattttcagc gacaaatttg ccttggacga tttgaaagaa ggcgacacgg tgcgcgtgtt
     2941 gtacgagagc cataatttcc gtggtcaaga ggtgtctact ggcaacatca tggctttgga
     3001 aattggcaaa gctggccgct tgtaccaagc ctattatttt gaccatggcg acaacactgg
     3061 tgcgtattac gaccaccgtg gcaaggcctt gagcaaaggc gaattcgcca aaattccagt
     3121 gaatgccacg cgcgtgagtt cgccttatgg cacacgttac catccgattt tgaaaaccta
     3181 ccgcatgcac agcggcatag actatgctgc gccaacaggc acgccgatta tggcgcctgc
     3241 cgatggtgtg gtgtcgtttg ccggtgtgaa aggtggctat ggcaatgcca tcatgttgaa
     3301 ccatcgcaaa ggcatggaaa ccttgtacgg tcacatgagc gcgtttgtgt cgggtgtgaa
     3361 tgcgggcaaa tcggtgaaag ctggtgatgt gattggtttt gtcggctcta caggccgctc
     3421 tacaggcccg catttgcatt atgaagtgcg catcaatggc caaatcgtcg atccatcaac
     3481 cgtggccttg cctgcccgta ctttgagcaa taccgagttg gcagcgttca aaggccgtgt
     3541 gaaatcatca gaccaaaaaa tcgcctcaat tcgtggtctg cctgtgatgg tggcgcaatt
     3601 ggattaaagg tttcaatgac ttaacaaagc ggcctgtaaa atgtttacag gccgcttttt
     3661 tgtggtgaaa aaggtggcac tttacaaaat tttgaattct tttgattaat ttactgttac
     3721 ttaacacggg tcttgggtag tatctcccct ttcgcaaatc attcattatt aaggataaat
     3781 catcaatgct gaagtcatcg atgacgggtt ctgttgccgc attggcattg atgctaccct
     3841 tatttgccct agcggcaccc aagccagttt ctgctttaca acgaaatcag ttcatattgc
     3901 aagaaaacca gtcacacaaa ccatcgctgt ttttaaataa ggcagatgct cagcaagaca
     3961 gcgaagtgca aatccaaaaa accgtgaata cccgttctgc gcgtgggctg tatgtcaacc
     4021 acacccccgc cacttcagaa tatggcaacc gcctgcatcc tgtgtccaaa cgctacaaat
     4081 tgcaccaagg cgatgattac gctgcaccag cgggcacgcc catcatggcg ccttctgcgg
     4141 gcatcatcac ttacatgggg cgcaaaaatg gctatggcaa caccgtgatg ttgcgccaca
     4201 gcagtggcca agaaacgctg tatgcgcaca tgagccgctt taataaaggt ttgaaagtcg
     4261 gcgatgtggt gcaaaacggt gacaaaatcg gctatgtggg ttcaacaggc atttccacag
     4321 gcccgcacct gcactttgaa gtgtgggtgg atggcaagcg agtgcgaccg tccagcgtga
     4381 aattgccgag caaggctttg aatgccagtg aagccgaatt gctgcgcact tacttgaagc
     4441 caacaggcaa acctgccaaa accatacagc tttaacacag tatcaacacc atccaaacca
     4501 caccgagttt ggatttgatg cggcctgtaa atgtttacag gccgcatttt attggcatga
     4561 ggctgattca ttaacacaga tttaaactat ttttaccacg ataccaacgt cattaacact
     4621 gtttaaacgt atgatggtgg ggtctgtcag actcaattca aatatcaagt caaatatcaa
     4681 ggaaaataaa atgaatgcaa tgaaaaccac tttgatgggt ttgaccgcgg ctgctgtttt
     4741 gggggtgtca actttggctg ttgccgaaac caaacctaca ccacaagcac gtccgcaact
     4801 cactgaacag caaaaagccc aaatgcaaga acgccatttt gaacgccgct atggcgattt
     4861 gaaattgagc aaatcacagc aaaccaaaat caaagcgatt gaagagcaat accgcccaca
     4921 agcgcgtgca caaatcagcg atgctgagcg tcaagcgttt gctgcgaatg ccgataaatt
     4981 gcgccaagcg cgtttgaatg tggttcaagg caagactttt aacgaagccg aagcccagca
     5041 agtgtttgca caagagcaag cggtgcgcaa tgaaatgcaa caaaaacacc agcaacaacg
     5101 cgcccaaatg gaattgacgc aattgcgcaa agagcatgcg attttccaag tattggataa
     5161 aaaacagcag caacaatact tagagcgtgc caaacaaccg cgtccgatga tggcacgcga
     5221 tggcggtaaa ggcatgaaag gcaaaccgca tcaccgtggt catggcccac gcccagctgt
     5281 tgcgccacaa gcggcaacgc ctgcaaaata aggcatgagt tttaaaccac accctgttgt
     5341 ctgctcggca acagggtgtt ttgtttggtg tcgcaattat ttggcaggac acacgtttgt
     5401 ggccggtttg tgtttacagg ccgcttatga cagcaataca aaacagatgg cgccatgatg
     5461 gtttacaggt cgagtttcgg ttacattaag cccacattca ggagagcaca tgggcacacc
     5521 cacagcgtat gcatttgtcg atttggaaac cacaggcggc agcgcccaac acgaccgtat
     5581 caccgagatt gcgattattc gggtggaggg cgagagcgtg acccgttacc aaagcctaat
     5641 caatccacag caacacatat cctatttcat tgaatccatc acaggcattt ccaatgccat
     5701 ggtcgccgat gcgcctgagt ttgccgaggt ggcagatgag gtgtggacat ggttgcaaga
     5761 ctgcgtgttt gtggcgcaca acgtgcgctt tgattatggc tttctcaagc aagcgtttga
     5821 gcgcgtgggc aaagattttc agcctgaaca attgtgcacc gtgaaactgt cacgccgcct
     5881 ctatcccgaa cacaaacacc atggtctaga ccaattgata gatcgccacg gcttgcgcat
     5941 gagccagcgc caccgcgcca tggccgatgc ggatgcgatt taccaatttt ggcaaatcgt
     6001 tcaccaagaa tttgctgccg atgtgttaca ggccgcttgt ttgaaggtga tgaacaagcc
     6061 tagcttgccc gtgtatctga atccactgag catagacacg ctctacaacg gctatggcgt
     6121 gtatttgatt tatggcgaca acgatttgcc gctctacata ggcaaaag
//
LOCUS       DAOMVK010000078         6070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_3495_length_6070_cov_3.261679, whole genome shotgun sequence.
ACCESSION   DAOMVK010000078 DAOMVK010000000
VERSION     DAOMVK010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 6070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6070
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3495_length_6070_cov_3.261679"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /locus_tag="PK856_04000"
     CDS             complement(<1..1048)
                     /locus_tag="PK856_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HRL01568.1"
                     /translation="MSLPFTIARTAAVHELNIVAKMANRHGLIAGATGTGKTVTLRKM
                     AECFSDAGIPVFLLDVKGDLSGIAEAGEHAGMVAKRMDEFGLSPDYLHRFPVRFWDVF
                     AEKGISLRVTISQMGPLLLSHLMGLNETQAGVMTLVFKVADDKAWHLIDLKDLRGMLK
                     YVSDNAKEFRGTYGNVSSASIGAIQRQLLQLEQDGADVFFGEPELNLDDWLQTEGSQG
                     VINILQGERLMRSPRIFSAFLLWLLAELFEQLPEVGDLPQPKFVMFFDEAHLLFDSAE
                     PALLQQIEQVVRLIRSKGVGVYFVTQNPLDLPDTVLGQLGNRVQHALRAFTPRDQKAV
                     KAAAETFRSNPDVDV"
     gene            complement(1050..1706)
                     /locus_tag="PK856_04005"
     CDS             complement(1050..1706)
                     /locus_tag="PK856_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRL01569.1"
                     /translation="MAKQGKRNTYDRIVDASLELFNTEGERKISTNHIAAHLSISPGN
                     LYYHFRNKDEIILQLFKRYRSELSEILNRSETPKDMNGMIDYFLEVFDVMWRYRFLFA
                     DVNTLLMRNSELSGEYQNFTWKQVSPMVHTHLQRLIDGGALKGEVLDIDALALNIWLI
                     CRYWFVFDGSLNNHQLDEKSKVRGLLQVFNLLRPYVRDEFMAQYNDGLDVLQHRLEAN
                     "
     gene            complement(1855..2874)
                     /gene="murB"
                     /locus_tag="PK856_04010"
     CDS             complement(1855..2874)
                     /gene="murB"
                     /locus_tag="PK856_04010"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HRL01570.1"
                     /translation="MNIEQDYALDELNTFGLKSQAQSFCLVHTEAELEKLVQSALFLQ
                     QSVSWLGGGSNIILAPYVNSFVIQLCNKGVEVIEENNDNVLVKAQAGEVWHEFVLKTL
                     SIGYNGLENLSLIPGHVGAAPVQNIGAYGVEVKDRIHSVICFDTYSREWQTFSKVDCQ
                     FQYRDSFFKHAENGRYVIWAVIFELSKRFIPNIQYGDLEEVSNRMSIGQALTAKTVSD
                     AVCSIRQQKLPDPHITGNVGSFFHNPIVDADVANELKQRYPDMPQYIQNDGRIKLAAG
                     WLIDQAGLKGHQIGQAAVHLKQALVLVNLGGATHTDVLDLSKHIQETIFTKFGVKLHP
                     EPNFW"
     gene            complement(2871..4244)
                     /locus_tag="PK856_04015"
     CDS             complement(2871..4244)
                     /locus_tag="PK856_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRL01571.1"
                     /translation="MLFDLNRFGAGTYKQEAAKIVHLAWPMFIAQIAQVGVGVVDTVM
                     AGRYGAEDLAAVALGTSLFMTLYISLMGVVTALNPILSQLYGAQKISDLSKVASQGVW
                     LAFILGIVGMLIMWALIAPLNHYFKLGDYVKHVFAYYMFWIAMALPFALVYRSLHAYA
                     SSLNRPKPMMWISLFGLCLSIPINYVLIYGLWGIPGLGGIGCAIGTILVFAINTVCLW
                     LYIRYDGYFKQFGRLSLAGGLDFPILKRIVVLGIPIGLSFLIEVSLFTFISILIANLG
                     VEYVASQQVVINISSLVYMVPQSLGAALSVCVGYAVGSGQMQRARYIAGVGLTIGLVA
                     SCVTVLVILIFRTPIVSLYTTDAAVITIGATLLVFNAVFQLPDAMQTIATGALRGYKV
                     TRLPMVVHVIAFWVFGLCLGYVLCFHFNMGIYGFWTALIFSLTLAAIAFIILLSRVSQ
                     RFMSKQA"
     gene            complement(4351..4911)
                     /gene="efp"
                     /locus_tag="PK856_04020"
     CDS             complement(4351..4911)
                     /gene="efp"
                     /locus_tag="PK856_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRL01572.1"
                     /translation="MKTAQELRAGNVFMVGSEPMVVQKSEYNKSGRNSAVVKMKLKNL
                     LTGAATESVYKADEKFDVVVLDRKQCNYSYFADPMYVFMDQEFEQYEIEAENMGDALN
                     YIVDGMEDVCEVTFYNGKAISVEMPTMVVREVEYTEPAVKGDTSGKVMKPARLIGGAE
                     ISVPAFVENGDKIEIDTRTNEFRKRA"
     gene            complement(4948..>6070)
                     /gene="earP"
                     /locus_tag="PK856_04025"
     CDS             complement(4948..>6070)
                     /gene="earP"
                     /locus_tag="PK856_04025"
                     /inference="COORDINATES: protein motif:HMM:NF021598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HRL01573.1"
                     /translation="SWRLAKQLQQEQQMHVVLWVDDVQALQALLPEVDVLAQYACYED
                     ITVVQWQDALVGELLLKTPAADVVIETFACDVPAEVRQWMVGKPIVWLNLEYLTAETW
                     VDDVHLLPSLQGNGVKKCFFCPGFSDKTGGVSYEQALLSQERPVSMEKQQQLRQQFGL
                     PSFADSVHVYVFGYADAMWPKWLRMWMQDKQKTVVWLAQGGLLADLQIHFPELQALQQ
                     VGDKVRLQQVTVCLVPFVAQSEFDDVLQAADVSVVRGEDSVLRALWQGRVFWWQIYRQ
                     DEHAHHIKLHAFWHRCLDNMKGLQAACFDGVWTAHQALSDELNGVRVLHESERAQAWE
                     AVMQHQAVLMAVLQAWREQLWQQQSLATQLANFMRLQLK"
BASE COUNT         1769 a         1604 c         1166 g         1531 t
ORIGIN      
        1 ccacatccac atcaggattg ctgcggaagg tttctgccgc cgctttcacg gctttttggt
       61 cgcgcggggt aaacgctcgc agcgcatgtt gcacccgatt gcccaattgt cccaatacag
      121 tatcgggtaa gtccaatggg ttttgggtga caaaatacac acccacgcct ttggaacgta
      181 tcaaacgcac cacttgctcg atttgctgta ataaagcagg ctcggcactg tcaaacagca
      241 agtgcgcttc atcaaaaaac atcacaaact taggctgcgg caaatcaccg acttctggca
      301 attgctcaaa taattcggcc aacagccaca gtaaaaacgc actgaaaatg cgcggtgaac
      361 gcatcaaacg ctcaccttgc aaaatattga tgacgccttg gctgccttct gtttgcagcc
      421 aatcgtctaa attgagttct ggctcaccga aaaacacatc ggcaccatct tgttccaatt
      481 gcaataactg gcgttgtatc gcaccaatac tggcggaaga cacattgcca taagtgccac
      541 gaaattcttt ggcattgtca gacacgtatt tcaacatgcc gcgcaaatct tttaagtcga
      601 ttaaatgcca agccttatcg tcggccactt tgaataccaa ggtcatcaca cccgcttggg
      661 tttcattcaa gcccattaaa tgcgacaaca acaacggccc catttgcgaa atggtgacac
      721 gcaatgaaat gcctttttcc gcaaacacat cccaaaaacg cacaggaaaa cggtgtaagt
      781 aatcagggct taggccaaat tcatccatgc gcttggcaac catgcctgca tgttcccccg
      841 cctcggcaat gcctgacaaa tcgcctttga catccaataa aaaaaccgga atgccagcat
      901 cgctgaaaca ttccgccatt ttacgtaaag tcaccgtttt accagtgcct gttgcacctg
      961 caatcaatcc atgtctgttt gccattttgg caacaatgtt caattcatgt acagcagcag
     1021 tgcgggcaat ggtaaacggt aaactcatat tagttagctt ccaaacgatg ttgtaatacg
     1081 tctaagccat cattgtattg ggccatgaac tcatcacgca catatgggcg caacaagttg
     1141 aacacttgca ataaaccacg cactttggat ttttcatcca attgatggtt gttcaaagaa
     1201 ccatcaaaca caaaccagta gcggcagatt aaccaaatgt tcaaggccaa agcatcaatg
     1261 tctaagactt caccttttaa cgcaccgcca tcaattaagc gctgcaaatg agtgtgtacc
     1321 ataggtgata cttgtttcca agtgaagttt tggtactcac ccgataattc gctgttacgc
     1381 atcagcaaag tgttgacatc ggcaaacaag aaacggtaac gccacatcac atcgaatact
     1441 tccaggaagt aatcaatcat gccattcatg tctttaggcg tttcgctgcg attgaggatt
     1501 tcagacaatt cagaacggta gcgtttgaat aactgcaaga tgatttcatc tttattgcgg
     1561 aagtggtaat acaaattacc tgggctgatg cttaagtgag cggcaatgtg gttggtactg
     1621 attttgcgct cgccctcagt gttaaagagc tccaaacttg catccacaat acgatcgtaa
     1681 gtgttgcgtt ttccttgctt agccatgtgt aaaacctttt tagtgtgtgt gtagtaaaaa
     1741 catctgctaa aacgtaactt tattaatatg taatatacat actgtaaagt ttgagcaaac
     1801 ccaatttggg tttactcctt gataccaacg ctctcccatc cattcagtgg tttgctacca
     1861 aaagttaggc tcaggatgca gcttgacacc aaacttggta aagattgtct cttggatgtg
     1921 tttggacaaa tccaaaacat cggtgtgtgt ggcaccgccc aagttcacca aaaccaaagc
     1981 ttgctttaag tgcactgcag cttgacctat ctgatgtcct tttaaacctg cttggtctat
     2041 caaccaacct gctgcaagct taattcggcc atcattttgg atatactgag gcatatctgg
     2101 atagcgttgt ttcaattcat tggcaacatc tgcgtcaaca ataggattgt gaaagaaact
     2161 tcctacattg cctgttatgt gtggatcagg caatttctgt tgacggatgc tacaaacagc
     2221 atcactgact gtttttgcgg tcagtgcttg tccaattgac atgcgattgg acacttcttc
     2281 caaatctcca tactgaatat ttggaataaa ccgtttactc agctcgaata ttaccgccca
     2341 aatgacatac cgtccatttt ctgcatgctt aaaaaaacta tcgcggtatt gaaactgaca
     2401 atctacttta gaaaaagttt gccattctct agagtaagtg tcaaaacaaa ttacagaatg
     2461 aattcgatct tttacttcaa caccgtaggc gcctatattc tgtacaggtg cagcccccac
     2521 atgaccagga atgagactaa ggttctctaa tccattgtat ccaatggaca aagttttaag
     2581 aacaaattca tgccacactt ctccagcctg agcttttact agcacattgt cattattctc
     2641 ttcaattact tcaacgcctt tgttacacaa ctgtattaca aaagaattta catatggagc
     2701 caaaataata ttactaccac cacccaacca tgaaacagac tgttgcaaaa acaatgcact
     2761 ttgcactaat ttttcgagtt cagcttcagt gtgaaccaga caaaaagatt gcgcttggct
     2821 tttcaaacca aatgtattca gttcgtctaa ggcgtaatcc tgctcaatat tcatgcttgc
     2881 ttgctcataa agcgctgact gacacgtgat aacaaaataa tgaacgcaat ggcagccaat
     2941 gttaatgaaa agattaaggc agtccaaaat ccgtaaatgc ccatgttaaa gtggaagcac
     3001 aatacataac ctaggcacaa tccaaacacc cagaacgcaa tgacatgcac caccataggc
     3061 aaacgggtca ctttatagcc cctaagcgca cctgtggcaa tggtttgcat cgcatcgggc
     3121 aattggaaga ccgcattgaa caccaagagc gttgccccga tggtaatgac agctgcatca
     3181 gtcgtgtaca gagaaacaat gggcgtgcgg aaaatcaata tcaccaaaac agtcacacaa
     3241 gaagccacca aacctatggt caatcccaca cctgcaatat agcgtgcccg ctgcatttgg
     3301 ccactgccaa cagcataacc aacacacacg cttaaggccg cacccaagga ctggggcacc
     3361 atataaacca gactactgat gttaataacg acttgctggc tggcaacata ctccacgccc
     3421 aagttggcaa tcaaaatcga aataaaggtg aacaaactta cttctatcaa gaacgataag
     3481 cctatcggaa tgcccaacac cacgatgcgt ttgaggatgg gaaaatccag gccacctgcc
     3541 aaactcaaac ggccaaactg tttgaaatag ccgtcataac ggatataaag ccacaaacac
     3601 actgtattga tggcgaatac caaaatggtg cctatggcac aaccaatacc acccaaacca
     3661 ggaatgcccc acaagccata aatcaacaca tagttaatgg ggatgctcaa gcacaggcca
     3721 aacaagctga tccacatcat gggtttcggt cggttcaaac tgctggcata ggcatgtaaa
     3781 ctgcggtata ccaaggcaaa tggtaaagcc atggcgatcc aaaacatgta ataggcaaat
     3841 acatgtttta cataatcgcc cagcttaaaa taatggttca gaggcgcaat caaagcccac
     3901 atgatgagca tacccacaat gcccaatata aaggccaacc acaccccttg actggcaacc
     3961 ttactcaggt cagagatttt ttgagcaccg tacaattggg acaaaattgg attcaatgcg
     4021 gtcaccaccc ccatcaaact gatgtagagc gtcataaaca agctggttcc cagtgctacc
     4081 gcggccaaat cctctgcacc ataacgaccc gccatcacgg tatcgaccac acctaccccc
     4141 acttgggcaa tttgggcaat gaacatcggc caagccaaat gcacaatctt ggctgcttct
     4201 tgtttgtaag tgcccgctcc aaagcggttt aaatcaaata acatcagctt cctgctttca
     4261 ggccgcatta gcggcttttt caaataaaac gacgctgcgt gtttgcacac tcgcagcgtc
     4321 gctcaatcca tgtaaacgct ttatcagaga ttaagcacgt ttacggaact cattggtgcg
     4381 ggtatcaatc tcgattttgt cgccgttttc aacaaacgct ggcacagaaa tttcagcgcc
     4441 gcctatcaag cgagctggtt tcatcacttt acctgaagta tcgcctttca cagctggttc
     4501 agtgtattcc acttcacgca ccaccatggt aggcatttcc acagaaatcg ctttgccatt
     4561 gtagaaagtc acttcgcaca catcttccat gccatcaacg atgtagttca aggcatcgcc
     4621 catgttttca gcttcgattt cgtattgttc aaactcttgg tccatgaaca catacatagg
     4681 atcagcgaaa tagctgtaat tacactgttt gcgatccaaa accaccacgt cgaatttctc
     4741 gtctgctttg taaaccgact cggttgccgc accagtcaac aggtttttca acttcatttt
     4801 caccacggca gagttacggc ctgatttgtt gtactcagac ttttgaacca ccataggctc
     4861 gctgcccacc ataaacacgt taccggcacg caattcttgt gctgttttca taatatttgt
     4921 ttccgcttaa aaaagattta aaacgcgcta ttttaactga agccgcatga aattggctag
     4981 ctgtgttgcc aaactctgtt gttgccacaa ttgctcacgc caagcttgca aaacagccat
     5041 caatacagct tgatgttgca taaccgcttc ccaagcctgc gcccgctcag attcatgcaa
     5101 tacccgcaca ccgttcaatt catcagacaa agcttgatgc gccgtccaaa caccatcaaa
     5161 acaagcggcc tgtaaaccct tcatattgtc caaacaacgg tgccagaatg cgtgtagctt
     5221 aatgtggtgt gcatgctcat cttggcggta gatttgccac cagaataccc gcccttgcca
     5281 caaagcccta agcacactgt cctcgccccg caccacgctc acatcagcgg cctgtaacac
     5341 atcatcaaat tcactttgcg ccacaaacgg caccaaacac accgtcactt gctgcaatct
     5401 caccttgtcc cctacttgtt gcaaggcttg cagctcaggg aaatggattt gcaaatcagc
     5461 caataaacca ccttgagcca gccacaccac ggtcttttgt ttgtcctgca tccacatgcg
     5521 caaccatttg ggccacatcg catcagcata gccaaacaca tacacatgca cactgtctgc
     5581 aaagcttggc aaaccaaatt gctggcgcaa ttgctgctgt ttctccatgc ttacaggccg
     5641 ctcttgtgac agcaatgcct gctcgtatga cacaccgcct gttttgtcac tgaagcctgg
     5701 gcaaaaaaag cattttttca ccccattgcc ttgcaatgaa ggcaataaat gcacatcgtc
     5761 cacccatgtt tcggcagtga ggtattccag attcagccac actatcggct tgcccaccat
     5821 ccattgccgc acttctgcag gcacatcgca ggcaaaggtt tctatcacca catcggctgc
     5881 aggtgttttc aacagcaatt cgcccaccag cgcatcttgc cattgcacaa cggtgatgtc
     5941 ttcataacaa gcatattggg caagaacgtc tacttcaggc aacaatgcct gcaaggcctg
     6001 cacatcgtcc acccacaaca ccacatgcat ctgctgttct tgttgtaatt gcttggccaa
     6061 gcgccacgac
//
LOCUS       DAOMVK010000079         6065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_3497_length_6065_cov_3.280865, whole genome shotgun sequence.
ACCESSION   DAOMVK010000079 DAOMVK010000000
VERSION     DAOMVK010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6065
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3497_length_6065_cov_3.280865"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..1488
                     /locus_tag="PK856_04030"
     CDS             28..1488
                     /locus_tag="PK856_04030"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRL01574.1"
                     /translation="MGLLNKLWQQWHHTQDEAHAVPVMADEDFHYAPHFIAGERLIVE
                     SNQVLEVINPCNSRAERRLYVASAATIQRAVQTAKENLPVWAAYAYLQRLYVIEKFKN
                     SFAVQQHILTEVLRGESGLNETEVAIELEQTLEAIVYALTAMQAKGSSTPAWGSTDVW
                     VQHAMQPVGVVAIISNEEQTLKSLVSEIVVALLCGNTLIVKPHPSHPLLPVLLGDWLA
                     QSGLPLGVYNVLQGDEEVAKALHVHPNVNAITYVLAQPAAKQAHVRQRQTILAHNYIV
                     VDEGMDVDKVWEQYLSSEALRLSVPIWIVLGSVTADVLQQKWQKHLASLPAMVLPTEQ
                     SKMRCSDFLQQAVGAGADLVVDGSQGDLPDKGWYMGMSLLDHVSPEMALHTEAPTRMA
                     VMLMRVPDLDAAIAIINATPDAGAASLFTESMTAMGLFKLQLQQVEVISFNRVESVPN
                     LNLPRGDIAHQWAVGGQHAARWHFYIRHQLIMGTAI"
     gene            complement(1626..1949)
                     /locus_tag="PK856_04035"
     CDS             complement(1626..1949)
                     /locus_tag="PK856_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRL01575.1"
                     /translation="MGVQTLSVAELKTWRDEGKDFVLLDVREDGEVAHANIAEHTHIV
                     MNLIPLRHNELPDDKPIVVYCHHGMRSMQVALFLEHAGFTDVYNLSGGIEAWSVQIDP
                     SVPRY"
     gene            complement(1949..2605)
                     /locus_tag="PK856_04040"
     CDS             complement(1949..2605)
                     /locus_tag="PK856_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HRL01576.1"
                     /translation="MDFAQARHNMVEQQIRPWDVLEFELLDVLSEIPREYFVLPEHQA
                     VAYADQALPLANGHAMPEPRIVAKMIQALKIKKTDRVLEIGTGSGYATALLSRLGQSV
                     MSLDLDAAQQERAQAALGKAGCTNVQLSVANGLKALANIGSFDVIYVGGGVADVPAEL
                     LHTLNEGGRLVVVLGQKRLMHATLISRKGNDYAREMLFETSVPALVDEQQLQKAEFEF
                     "
     gene            complement(2793..4259)
                     /locus_tag="PK856_04045"
     CDS             complement(2793..4259)
                     /locus_tag="PK856_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRL01577.1"
                     /translation="MLEIFSSLSPAMVALLILALGLVFFFEFINGFHDTANAVATVIY
                     TQSMKPVHAVIASGTFNFLGVVTGGLAVAYAIVNLLPIDLLASGNTQVLLAMVFALLL
                     AAIIWNLGTWYFGLPASSSHTLIGAILGVGVANALMEGQSFTTGVNWNKAMDVGASLL
                     FSPMAGFILAYVMLAVLRKLMPIGKIHQTPYLRNGVRGKKHPPFWTRFLLILSAMGVS
                     FAHGSNDGQKGIGLAMLTLICILPSHYVVNSSINQYDLERSLSSVTRLESFIQQNQTS
                     IQAAFPVTPSLATGKEKVHCQPGDALTYGQNVRQLMAGSHDISHFSTDQRWALRTNLL
                     CLEDSAKKLAAMPGNTPAQKAYLQQVGKDFALPVEYAPLWVIIAIALSLGIGTMIGWK
                     RVVTTVGEGIGKSHMTYAQGVCAQVTAAVGIAGANLFGLPVSTTQILSSAVAGTAVAN
                     KSGLQWSTIRNILLAWIFTFPVAMGIAGGLYFLIRMVF"
     gene            complement(4426..5181)
                     /gene="pstB"
                     /locus_tag="PK856_04050"
     CDS             complement(4426..5181)
                     /gene="pstB"
                     /locus_tag="PK856_04050"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HRL01578.1"
                     /translation="MQATISAKNFNFYYGDFHALKDINLDIETKKVTAFIGPSGCGKS
                     TLLRTFNRMYDLYPGMRAEGELNLEGKSILGKDIDLNLLRAKVGMVFQKPTPFPMSIY
                     DNVTFGVKLYEKLSRSDLDDRVEWALKKAALWGEVKDKLKQSGNSLSGGQQQRLCIAR
                     AVATKPEVLLLDEPTSALDPISTAKVEELVTELKDDYTIAIVTHNMQQAARVSDYTAY
                     MYLGELVEFDDTKIIFTKPSQKATEDYITGKFG"
     gene            complement(5200..6051)
                     /gene="pstA"
                     /locus_tag="PK856_04055"
     CDS             complement(5200..6051)
                     /gene="pstA"
                     /locus_tag="PK856_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HRL01579.1"
                     /translation="MNNALYKRRLLINKFNLTMAWATMAFGLFWLAWILFTLFGQGID
                     GLLDIKTYTMDTPPAGTEGGLRNAIVGSLMITFFGLLVGTPIGILAGIYLAEFGHKSW
                     LASATRFMNDILLSAPSIVICLFIYGLMVVPMGHFSGWAGSMALSLLVIPVVIRTTEN
                     MLRLVPNSLREAAYALGTPKWKLVTMVTLRAAKAGVLTGVLLAFARVSGETAPLLYTA
                     LNNQFFSTNMNAPIANLPNTIFQFAMSPYQDWQRLAWAAALLITLTVLIANIAARFLS
                     RQSASKH"
BASE COUNT         1602 a         1441 c         1532 g         1490 t
ORIGIN      
        1 caatgtcata aattctgagg agcggccatg ggtttgttga ataaattgtg gcaacagtgg
       61 caccacacgc aagacgaagc ccatgccgtg cctgtgatgg ccgatgagga ttttcattac
      121 gcgccgcatt tcattgcggg tgaacgcctg attgtggagt ccaatcaagt attggaagtc
      181 atcaacccat gtaacagccg tgccgaacgc cgtttgtatg tggccagtgc agccaccatc
      241 caaagagcgg tacaaactgc caaagaaaac ctgcccgtat gggcggccta tgcctatttg
      301 cagcgtttgt acgtcataga aaaattcaaa aacagctttg ccgtacagca acacatcctc
      361 acagaggtgt tgcgtgggga gagcggcctg aacgaaaccg aagtagcgat tgagcttgaa
      421 caaaccctcg aggccattgt gtatgcctta accgccatgc aggccaaggg cagctctacg
      481 cccgcttggg gcagcaccga tgtgtgggtg cagcatgcca tgcagccagt tggtgtggtg
      541 gcgatcatca gcaatgaaga acagacctta aaatccttgg tcagtgagat tgtggtggcc
      601 ttgttgtgtg gcaacacctt gattgtgaaa ccgcatccca gtcatccgtt gttgcccgtg
      661 ttgttgggcg actggttggc gcaatcgggt ttgcctttgg gcgtttacaa tgttctccaa
      721 ggggatgaag aggttgccaa agccttgcat gtgcatccca atgtcaatgc cattacctat
      781 gtgttggctc aacctgctgc aaaacaagcc catgtgcggc aacgccaaac cattttggcc
      841 cacaattaca ttgtggtgga tgaaggcatg gatgtggaca aggtgtggga gcagtatttg
      901 tccagtgagg ccttgcgttt gagtgtgccc atttggatcg tgttggggag cgtcactgcc
      961 gatgtattgc agcaaaaatg gcaaaagcat ttggccagtt tgccagcaat ggttttgcct
     1021 actgagcaaa gcaaaatgcg ctgcagcgat tttttacagc aagcggtggg tgcgggtgcc
     1081 gatttggtgg tggatggcag ccaaggcgat ttgcctgaca aaggttggta catgggcatg
     1141 agtttgcttg accatgtatc gcctgaaatg gcgttgcaca cagaagctcc gacgcggatg
     1201 gcggtgatgt tgatgcgtgt acctgatttg gatgcggcca ttgccatcat caacgcgacc
     1261 cccgacgcag gggccgccag tttgttcacc gaaagcatga ctgcaatggg tttgtttaag
     1321 ctgcaattgc agcaagtgga agtcatcagt tttaaccgcg ttgaaagcgt gcccaatttg
     1381 aacctgcctc gtggcgacat cgcccaccaa tgggcagtgg gtggacagca tgcggcgcgc
     1441 tggcattttt atattcgaca ccaattgatt atgggcacgg caatctgatg catggattcg
     1501 atacaaacaa gcggcctgta aactgttaca ggccgcttgt ttttgttgga taacgattga
     1561 tagcgacaaa aaacaaaaca ccgtgtgtca cacggtgttt taagctttat gggtatgtgt
     1621 aagcattaat agcggggcac acttgggtcg atttgcacag accaagcttc aatgccgccg
     1681 ctgaggttgt acacatcagt gaaaccagca tgttccaaaa acaaagccac ttgcatgctg
     1741 cgcatgccgt gatggcaata caccacgatg ggtttgtcgt ctggcaactc attgtggcgc
     1801 aggggaatca aattcatgac aatgtgggtg tgctcagcga tgttggcatg cgccacttcg
     1861 ccatcttcac gcacatccaa tagcacgaaa tctttgccct catcacgcca agttttgagt
     1921 tcggccacac ttaaggtttg tacgcccatt agaattcaaa ttctgctttt tgcaattgtt
     1981 gctcgtcaac caaagcgggt actgaggttt caaacaacat ttcgcgggca tagtcattgc
     2041 ctttgcggct gattaaagtc gcgtgcatca agcgtttttg acccaaaacc accaccaaac
     2101 ggccaccttc atttaaggtg tgcagcaatt ccgcaggcac atcagccacg ccgccaccca
     2161 cataaatcac atcaaatgag ccgatgttgg ccaaggcttt caagccattg gcaacgctca
     2221 attgcacatt ggtgcaacct gctttgccca aagctgcttg agcgcgctct tgttgggcgg
     2281 catccaaatc caaactcatg acactttggc ccaaacgtga caacaaagcg gtggcgtagc
     2341 ctgagccagt gccaatttcc aacacgcgat cggttttttt gattttcaaa gcctgaatca
     2401 ttttggccac gatgcgcggt tctggcatgg cgtgaccgtt tgccaaaggc aaggcttggt
     2461 ctgcataggc cacagcttgg tgttctggca acacaaaata ctcacgcgga atttcagaca
     2521 atacatccaa taattcaaat tccaacacat cccaaggacg aatttgttgt tccaccatgt
     2581 tgtgtctggc ttgcgcgaaa tccataatta ctccgtaaaa taagccgcac aagcggcatg
     2641 tttaaacaat gacgctgatt atcccataaa aggcaagcac ttgcatgtgg atgctgcaat
     2701 aaaaagcggc ctgtaaactt tacaggccgc ttttgcgtgt gaatcaaact gtgcgtattc
     2761 aatgacaaga tgtacaaaac actttaggtg aattaaaaca ccatgcggat taagaagtac
     2821 aaaccgccag ctatgcccat ggccacaggg aaggtgaaaa tccacgccag taaaatattg
     2881 cggatggtag accactgtaa acctgatttg ttggccacag ccgtgcctgc tactgcgctg
     2941 gacaaaatct gtgtggttga tacaggcaag ccaaacaagt tggcccctgc aataccgacc
     3001 gctgccgtca cttgcgcgca cacgccttgt gcataagtca tgtgcgattt gcctatgcct
     3061 tcacccacgg tggtgaccac gcgtttccaa cctatcatgg tgcctatgcc caaagacaag
     3121 gcgatggcaa tgatgaccca taagggtgcg tattcaactg gcaatgcaaa atctttgccc
     3181 acttgctgca aataggcttt ttgcgcaggg gtgttgccag gcatagcggc taattttttg
     3241 gccgaatctt ctaaacacaa caaattggtg cgcaaggccc aacgctgatc ggtactgaaa
     3301 tggctgatgt catggctgcc tgccatcaat tggcgcacat tttgtccata cgttaaggca
     3361 tcacctggtt ggcaatggac tttctctttg cccgtagcca agcttggggt cacaggaaat
     3421 gcggcctgta tgctggtttg gttttgttga atgaacgatt ccaaacgtgt taccgaagac
     3481 aatgagcgtt ctaagtcgta ttggttgatg ctggaattca ccacgtaatg cgatggcaaa
     3541 atgcaaatca aggtcaacat ggccaagcct atgccttttt gaccatcgtt agaaccgtgg
     3601 gcaaaactca cgcccatggc tgacaaaatc aataaaaagc gcgtccaaaa cggtgggtgt
     3661 ttcttaccgc gcacgccgtt acgcaaataa ggggtttggt gtatcttgcc tatgggcatg
     3721 agtttgcgca gcacggcaag catcacataa gccaagataa agccagccat aggcgagaag
     3781 agcagggaag cacccacgtc catggctttg ttccagttca cacctgtggt aaacgattga
     3841 ccttccatca aagcattggc cacgcccaca cccaagatgg caccgattaa ggtgtgcgag
     3901 ctggaagcgg gcaagccaaa ataccatgtg cccaagttcc aaatgatggc ggctaacaat
     3961 aaggcaaaca ccatggccaa caatacttgt gtgttgcctg aggccaataa gtctatcggc
     4021 aacagattca caatggcgta agccactgcc aaaccacctg tgaccacacc caaaaagttg
     4081 aatgttcccg aggcaattac tgcatgtaca ggtttcatgg attgggtgta tatgactgtg
     4141 gccaccgcgt tggcggtgtc gtggaagccg ttaatgaatt caaagaagaa taccaagcct
     4201 aaggccagta tcagcagagc caccatggcg ggtgacaaac tggagaatat ttctaacata
     4261 atcaatgcat atcgtcgtaa tgggcgtgat tgttgtgctt ttaaacctga gggtcaagca
     4321 gaatattgtt gcaccttggt taatttgttg tttttaaaaa aagaaattag gcgtcccaag
     4381 ccttctggac gccttgattt gagtcaatta ttatgaagtg aataattagc caaatttacc
     4441 ggtaatgtag tcttcagtag ctttttggct gggtttggtg aagatgattt tggtgtcatc
     4501 aaactctacc aattcgccca aatacatata agcagtgtaa tcagacacac gagccgcttg
     4561 ttgcatattg tgggtaacga tggcgatggt gtaatcgtct tttaattcgg taaccaattc
     4621 ttcgactttg gccgtagaaa tcgggtctag agcggaagtg ggttcatcca acaataaaac
     4681 ctctggtttg gtggccactg cgcgggcgat gcacaaacgt tgttgttggc cgcctgacaa
     4741 ggaattgcct gattgtttca atttgtcttt gacttcgccc cacaaagcgg ctttttttaa
     4801 agcccactca acacggtcgt ccaaatcact gcggctgagc ttctcgtaca gtttcacacc
     4861 aaaagtcaca ttatcgtaga tagacatggg gaatggcgtc ggtttttgga acaccatgcc
     4921 cactttggcg cgcagcaaat tcaaatcaat gtctttgccc aaaatggatt tgccttccaa
     4981 attcaattca ccttctgcgc gcatgcctgg gtacaaatcg tacatgcggt tgaatgtgcg
     5041 caataaggtg gatttaccac agccagatgg gccaatgaag gcagtgactt tcttggtttc
     5101 aatgtccaag ttgatgtctt ttaaggcgtg aaagtcgcca tagtaaaaat taaaattttt
     5161 cgcagaaatg gtagcttgca tgttaaatat cctttgtcct taatgtttag aagcactttg
     5221 gcggcttaag aagcgagcgg cgatgttggc gattaacacg gttaaggtaa tcaacaaggc
     5281 ggcagcccaa gccagacgtt gccaatcttg gtaaggactc atggcaaatt ggaaaatggt
     5341 gttcggtaag ttggcaatcg gcgcattcat gttggtgctg aagaactggt tgttcagcgc
     5401 agtgtagagc aaaggcgcgg tttcgcctga gacgcgggca aaagccagca acacacctgt
     5461 taacacacct gctttggcag cacgcaaagt taccatggtc accaatttcc atttcggtgt
     5521 acccaaggca taagcggctt cacgcaagct gttgggcacc aagcgcaaca tgttttctgt
     5581 ggtgcgtatc accactggaa tcaccagcaa agacaaggcc atcgagcccg cccaacctga
     5641 gaagtggccc ataggcacca ccatcaagcc gtaaataaac aagcaaatca cgatggatgg
     5701 ggctgaaagc aaaatgtcgt tcatgaagcg ggtggcagag gccagccaag atttgtggcc
     5761 gaactcagcc aagtaaatgc ccgctaaaat gccgatgggt gtgcccacca acaagccaaa
     5821 aaaggtaatc atcaagctgc cgacgatggc attgcgcaaa ccaccttcgg tgccggcagg
     5881 cggggtgtcc atggtatagg ttttgatgtc gagcaagcca tcaatgcctt gaccaaacag
     5941 ggtaaacaaa atccacgcca gccaaaacaa accaaaggcc atggttgccc atgccatagt
     6001 gaggttgaac ttgttaatca gtaagcggcg tttgtataag gcgttattca tgttgtttcc
     6061 ttaat
//
LOCUS       DAOMVK010000080         6017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_3539_length_6017_cov_2.900537, whole genome shotgun sequence.
ACCESSION   DAOMVK010000080 DAOMVK010000000
VERSION     DAOMVK010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 6017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6017
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3539_length_6017_cov_2.900537"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..1720)
                     /locus_tag="PK856_04060"
     CDS             complement(161..1720)
                     /locus_tag="PK856_04060"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HRL01580.1"
                     /translation="MTALLAVEQVNAYFGNKQVLFDVDLAVAAGQKVAIVGESGSGKT
                     VLAQGIMRLNPDVRLTGNIRYQEQDLLAMPEKQLAKIRGRDIGMVFQEPMTALNPVYT
                     VGNQIAEVYTQHLGLTRKQAWQAAIDILRETGIQDAANKVKAYPFQLSGGQRQRAMIA
                     MAVAASPKLLIADEPTTALDVAVQAQIVQLLQRLQAEYQMALLYISHDLRLVERFADE
                     VMVMQQGRVVEHAPSAVLFQQPEHAYTVELLNAKPEIMCNPNVDDTQPILAVQDLRVE
                     VKQKSSWFQSKSICLLQPVSFTLHQGETIGIVGESGSGKTTLAKALMRLMPAIGKAVI
                     GGKDWQAMSMAELTRERQHMQMVFQDPFSAFNPRMNMAEALSEPLLIQKMRLTEAELE
                     QRLLQALDDVGLPHDSLSRYPHEFSGGQRQRLAIARALVVRPQVLILDEPTSALDVRL
                     QKHILRLLAKLQQQYGLSMLIISHDLDVIAALSQRMLVLQQGQVVEQGEVAAIFVKPN
                     HAYTKELLKHR"
     gene            1877..2323
                     /locus_tag="PK856_04065"
     CDS             1877..2323
                     /locus_tag="PK856_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01581.1"
                     /translation="MKLSHFSMFASALVMTMGATHLQAAPIKDSSTEMDQASQAAVTV
                     YRNKGNPGLQQAIADCYKSVGYATPFCVYMDVAGREIDFSVAQAYKELGQNVAPHAYF
                     QSKAFGERVASFYILHRQTRKESNEHIQKIQPQIKKRVEKILFPNG"
     gene            complement(2504..3190)
                     /locus_tag="PK856_04070"
     CDS             complement(2504..3190)
                     /locus_tag="PK856_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01582.1"
                     /translation="MKCWYQTVALGLGILALAACAIKFPPMPVMTTSQDEIRGAFSSS
                     DKLYLIGRARDYELPIAPFERYKQLADSHLRGNIVCAKITNARLYATTEKPNEFSATY
                     SVLLKGKNITPADMETYKLKRLDIDTTDSVAEPNIWYKLTDGGCPQLGHVKKESFYIA
                     YFSAQGRSQFLRNREQALNQSKLPQPLPMTVEKYIDPLPKARNNSVTDSAEVVVKAPF
                     YLLGIMFGGM"
     gene            complement(3346..4572)
                     /locus_tag="PK856_04075"
     CDS             complement(3346..4572)
                     /locus_tag="PK856_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HRL01583.1"
                     /translation="MNSFFSVWRRERPEHSWFAVITLSIAAFIFNTTEFVPVGLLSNI
                     ASSFDMDVAHTGLIMTVYAWAVTLFSLPLTMLSARFERRGLLAFLFVLFVASHVLAAF
                     AWDFTTLLIARLGIAAAHAVFWAITIPMAVRLAPEGKKAKALSLIVTGSSLATVLGVP
                     LGTVVGQHLGWRFTFGAIGGIAAMILLLLLMVLPRLPSTVSGNISVLPKLLKRPTLVY
                     VYATLALVVGAHFTAYTYISPFLHQIGGMGDGLVVTVLFVVGIAGILGGIVFARQLDS
                     HPLRLLVIPMGMLLLCLLALHMASLNVVSMLVLALVWGMSITILGMVLQNKVLEVAPD
                     ASDIGIAMFSGVYNIGIGGGALLGGLVISHLGLPFIGYLGSMLAVLAVLLFGVVGNQL
                     WLQAVRNDKKKNRPVE"
     gene            4649..4954
                     /locus_tag="PK856_04080"
                     /pseudo
     CDS             4649..4954
                     /locus_tag="PK856_04080"
                     /inference="COORDINATES: protein motif:HMM:NF020382.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
     assembly_gap    5422..5431
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5582..5758
                     /locus_tag="PK856_04085"
     CDS             5582..5758
                     /locus_tag="PK856_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01584.1"
                     /translation="MKKTYSVLATLATMALLSACANTQSSNADTSTASAPPPVVQESA
                     SPASASDANVFQGE"
     gene            5762..5947
                     /locus_tag="PK856_04090"
     CDS             5762..5947
                     /locus_tag="PK856_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01585.1"
                     /translation="MKTIFSMLLAAILITSLTAYANTINTDAAISQNVTEASQVQAST
                     PAASSTPASGAARLLDQ"
BASE COUNT         1688 a         1614 c         1272 g         1433 t
ORIGIN      
        1 aaatttaaga ttacacccga tgcgatttca ttggcaagga ttaattcagg aaaatctaaa
       61 ataaattatt cttgtgtgga tttgcaaata ttgagggcta aaacctcgct aggaaaatgc
      121 agctgtaggc agtaaaaatc tgcatgctgc cacgctgtga ttatctgtgc ttgagcaact
      181 ctttggtata ggcatgattc ggtttcacaa aaatggcagc cacctcacct tgttccacca
      241 cttgcccctg ttgcaacacc aacatgcgct gcgacaaagc ggctatcaca tccaaatcat
      301 ggctgatgat gagcatgctc agcccatatt gctgctgtaa tttggccaat aagcgcagaa
      361 tgtgtttttg taaacgcaca tctaaggcac tggtcggctc atccaaaatc aacacttgtg
      421 gtcgcaccac caaagcgcgg gcaatcgcca agcgttggcg ctgcccaccc gaaaactcat
      481 gcggataacg gctcaagctg tcgtgcggca aacccacatc gtctaaggct tgcaataagc
      541 gctgttctag ctctgcctca gttaagcgca ttttttgtat caacaagggt tcacttaagg
      601 cctcagccat gttcatgcgt ggattgaaag cagaaaatgg gtcttgaaac accatttgca
      661 tgtgctggcg ctcacgcgtg agctcggcca tgctcatggc ttgccagtct ttgccaccga
      721 ttacggcctt gcctatggca ggcatcaggc gcatcaaagc cttggccaaa gtggtcttac
      781 cactgccaga ttcacccact atgcctatgg tttcgccttg gtgcagcgtg aagcttactg
      841 gttgcagcaa acaaatagac ttggattgaa accacgacga cttttgcttc acctctactc
      901 tcaagtcctg taccgccaat atcggctgcg tgtcatccac gttgggattg cacatgattt
      961 cgggtttggc attgagcaat tcaacggtat aagcgtgttc aggctgctga aacaaaacag
     1021 cgctaggagc atgctcaacc acacgccctt gctgcatcac catcacttca tcggcaaaac
     1081 gctcgaccaa ccgcaaatca tggctgatgt agagcaaagc catctgatac tcggcctgta
     1141 aacgctgtag caattgcaca atctgcgcct gcaccgccac atccaaagcc gtggtaggct
     1201 catccgcaat caataattta ggactggcgg ccactgccat ggcgatcatc gcccgctggc
     1261 gctgccctcc tgaaagctga aacggatagg ctttgacttt gttcgcggcg tcttgtatgc
     1321 cagtttcgcg caaaatatca atggccgctt gccacgcttg tttgcgggtc aagcccagat
     1381 gctgggtata gacttcggcg atttgattgc ccacagtgta aacaggattg agcgccgtca
     1441 tcggctcttg aaacaccatg ccgatgtcgc gacctcggat tttcgccaat tgcttttcag
     1501 gcatggccag caaatcctgc tcttgatagc gaatattgcc agtaaggcgc acatcggggt
     1561 tcagacgcat gatgccttgt gccaacacgg ttttgccgct gcccgattcg cccacgatgg
     1621 ccactttttg ccccgctgcc accgccaaat ccacatcaaa caacacttgc ttgttgccaa
     1681 aataggcatt gacttgctcc actgccaaca acgctgtcat gcacttcccc tttattgttg
     1741 ctgactttat agcagctgcg gtttgtttga acaagttaag ccgatttact tgtgtgtgta
     1801 agctgcttat gatgctgaag ctgtgaaata gccaaccctt aactaatagc attatgtcaa
     1861 ttaaagtagg aataatatga aactcagcca tttttctatg ttcgcttcag cattggtcat
     1921 gacgatgggc gccactcatt tacaggccgc accgattaaa gacagcagca ccgaaatgga
     1981 ccaagccagc caagcagcgg tcacggttta ccgcaacaaa ggcaatccag gattgcaaca
     2041 agccatcgcc gattgctaca aatcggtggg ctatgccacg ccgttttgcg tgtacatgga
     2101 tgtggctggc cgtgaaatcg acttcagcgt ggcgcaagca tacaaagaat tggggcaaaa
     2161 cgtggcccca cacgcttatt ttcaaagcaa ggcattcggt gagcgcgtgg cttcgtttta
     2221 cattttgcac cgccaaaccc gcaaagaatc caatgaacac atacagaaaa tccagccgca
     2281 aatcaaaaaa cgggtggaaa aaatcttgtt tcccaatggt tgatgagtca gtgatttgat
     2341 tgcatcactc gcatgaaaag cggcctgtaa atgtttacag gccgctttat taatgccaaa
     2401 cacatggcta tacatactgt acatactgtt tcaagcaatt acattgataa ggggtgcgcc
     2461 aagcccacat tcagtcatat caccgcccta ccgaacgaaa ccatcacatc ccaccaaaca
     2521 taatgcccaa cagataaaac ggtgctttca ccaccacctc agccgaatct gtcacactgt
     2581 tgttacgggc tttgggcaat ggatcgatgt atttttccac ggtcatcggt agtggttgtg
     2641 gcaattttga ttggtttagt gcctgttcac ggttgcgcag aaattggctg cggccttgtg
     2701 ctgagaaata agcaatgtaa aacgattctt ttttcacatg acccaattga ggacagccgc
     2761 catcggtcaa tttataccaa atattgggtt cggccactga atcggtggtg tctatatcca
     2821 agcgtttgag cttgtaagtt tccatgtcag caggcgtgat atttttgccc tttagcaaca
     2881 cagaataggt agcactaaat tcattcggtt tttctgttgt ggcgtacaaa cgggcattgg
     2941 tgattttggc gcagacgatg ttcccgcgca aatggctgtc tgccagttgt ttgtaacgct
     3001 caaatggcgc aatcggcaat tcataatcac gcgctcttcc tatcaaatac agcttgtctg
     3061 aagatgaaaa agccccccga atttcgtctt gtgatgtcgt catcacaggc atgggtggaa
     3121 atttaatggc gcaagcagcc aatgccaata tgcccaagcc caaagccact gtttgatacc
     3181 aacacttcat gtcaaccttt ctaaaaacac ccgcacttta aatgtgctcc accaaacact
     3241 gctcaaggtc aatcagtcag caacgtattc cagagaaact agggttggct cgctcagtct
     3301 gccaagaaca aaaacttcgc atcgtcattt tgaccagcgc tgctgtcatt caacaggccg
     3361 atttttcttt ttgtcattgc gcactgcttg caaccacaac tgattgccca ccacgccaaa
     3421 cagcaacacc gccaataccg ccaacatgct accaaggtaa ccgataaacg gcaggcccaa
     3481 gtgggaaatc accaaaccac cgagcaaagc cccaccacct atgccgatgt tgtacacgcc
     3541 tgaaaacatg gcaataccta tgtctgaagc atcaggcgcc acttccaata ctttgttttg
     3601 caacaccatg cccaaaatgg tgatggacat gccccacacc aaagccaaca ccagcatgct
     3661 gaccacattg agactcgcca tgtgcaaagc cagcaaacac aatagcaaca tgcccattgg
     3721 tatcaccaac aagcgcagtg gatggctgtc cagttggcgc gcaaacacaa tgccgcccaa
     3781 tatgcccgca atacccacca caaacaacac agtcaccacc aagccgtcac ccatgccgcc
     3841 tatttgatgt aaaaacggcg agatgtaagt ataagccgtg aaatgtgccc ccaccaccaa
     3901 agccaaggtt gcgtatacat acaccaaagt cgggcgtttt aacaatttgg gcaaaacact
     3961 gatgttgccc gaaaccgtac tcggcaaacg cggcaatacc attaacagca gcaacaaaat
     4021 catcgcggca atgccaccga tggcaccaaa ggtaaagcgc caacccaagt gttgtccaac
     4081 caccgtcccc aagggcacac ccaatactgt cgccaacgat gagcccgtga caatcaagct
     4141 caaagccttg gctttcttgc cttcaggtgc caagcgcacc gccatcggaa tggtaatcgc
     4201 ccaaaacacc gcatgtgccg ctgcaatgcc caagcgcgca atgagtaaag tggtgaagtc
     4261 ccacgcgaac gcggccaaca catggctggc cacaaacaac acaaacaaaa aggccaacaa
     4321 accacgcctc tcaaaacgcg cgctcagcat ggtcagcggc aaagaaaaca gcgttaccgc
     4381 ccaggcatac accgtcatga tgaggccggt gtgggccaca tccatgtcaa aactgctggc
     4441 aatattggac aacaagccca caggcacaaa ctcggtggta ttgaaaataa aggctgcaat
     4501 agacaaggta atgacagcaa accatgagtg ttcaggccgc tcacgtcgcc atacagaaaa
     4561 aaatgaattc attgttatag aagaaaacac ggcagtcaga aacagcctat actgttgcca
     4621 ccatgattct taatagagaa aataatccat gattgtactg caaattgatt tccccagcag
     4681 cggcccattc ggcgcagaca tgagccaagc tttcatacct ttggcccaag acatcgctca
     4741 agaagcgggt ttgatttgga aaatctagac ggagaatgca gccgaacaca ccgcaggtgg
     4801 tatttatctg tttgctgatg aggcactggc tttggcgtat ttgaccaagc acactgctcg
     4861 cgtggaatct tttggcatcc aaaacatccg cagcaaactc tttaccatca acgaagcctt
     4921 gtccgccatc aatcacttcc aagtccctgc ttaagctgtt tgcctgaagc aagcaaagca
     4981 tagctttgat caaagctgtt cattaaagac cttaagtcac atatgcggcc tgtaaacatt
     5041 tacaggccgc atttcgttat tcttaatatg ctttaaatat gattccaaga agcacctcca
     5101 taattgccga ctggtcatta caacgccaat ctcacattgt gtttatacct ttttatacgt
     5161 tctcagttga gctgaaccat acagactatc catcggcaat ttcagcttgt ctttaagtca
     5221 cagattgcac tgcaacgatt aaatattgca atgcaacatt aatttttcat agtttaaagc
     5281 atctactcaa atcattgata cgcaagcgat tacaccactt gaacacaatg agcacacaca
     5341 aacttcataa tgtaggtttt cgtcaaaacc ctgccatttt tttacaaaac agtcgatttt
     5401 tcagttaaaa atgccaattt tnnnnnnnnn naatacacgt caaagtgggc gcatttttct
     5461 tgcttggccc aagggttctt gcctaagctt cccctcaagc aaaacaagca aaaccgccta
     5521 tttgcctact cagcttcaat gagtttttta acaaaagtat gaagacaaaa ggactgtaag
     5581 catgaaaaaa acgtactctg tattggccac tcttgccacc atggctttgt taagcgcttg
     5641 tgccaacacc caaagcagta acgcggacac cagcaccgcc agcgcccctc caccagttgt
     5701 acaagagtca gccagtccag ccagcgcgtc tgacgcgaat gtgtttcaag gagaataatc
     5761 catgaaaacg attttcagca tgctgcttgc agccattttg attacctcat tgacagctta
     5821 tgccaatacc atcaatactg acgctgcaat ttcgcaaaac gtcactgaag ccagtcaagt
     5881 tcaagccagc actcctgctg ccagcagcac ccccgcctca ggagcagcgc gcttgctaga
     5941 ccagtaagga agccaaaatg aattcatacc tacgtaattt tgccgtattg accaccgccg
     6001 caactttggc agcatgt
//
LOCUS       DAOMVK010000081         6008 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_3547_length_6008_cov_3.158576, whole genome shotgun sequence.
ACCESSION   DAOMVK010000081 DAOMVK010000000
VERSION     DAOMVK010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3547_length_6008_cov_3.158576"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PK856_04095"
     CDS             complement(<1..195)
                     /locus_tag="PK856_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01586.1"
                     /translation="MNLSEREELRLVLLRDASIGHFMWLDRWQVSYPQVIAAECSAHE
                     ALNLQNQRLQAAIEPEQDFPL"
     gene            complement(195..971)
                     /locus_tag="PK856_04100"
     CDS             complement(195..971)
                     /locus_tag="PK856_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRL01587.1"
                     /translation="MFRIISANVNGIRSAASKGFIEYIQASGADIVCVQELKAQEDKL
                     TDVLKKPNGMHGVWHCAQKAGYSGVAIYSKATPDRVQYGLGIEAFDNEGRYIRADFDD
                     KNLSVVSLYLPSGTSAEERQTYKYEFLDAFLPVLEGLLAEGRHIVVCGDWNIAHNNID
                     LKNWKGNQKNSGFLPEERAWLTKLLEQVGWVDTWRKLYPDNPGYTWWSNRGQAYAKDV
                     GWRIDYHMTTPSLYALAREAHVYKDEKFSDHAPLVVDYDL"
     gene            1053..1694
                     /gene="pyrE"
                     /locus_tag="PK856_04105"
     CDS             1053..1694
                     /gene="pyrE"
                     /locus_tag="PK856_04105"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRL01588.1"
                     /translation="MSDFRQQFLKFALEQEVLKFGEFITKAGRPSPYFFNAGLFNDGN
                     GILQLSRFYADAIIASGVEFDMLFGPAYKGIILAAATSMALAEKGVNVPIAYNRKEAK
                     DHGEGGVLVGAPVQGRVLIIDDVISAGTSVRESVKLIEAEGATPAGVVIALDRMEKGT
                     GETSAVQEVEQQYGLPVVSIANLHDLLAMLADDSTLQTYLVAVQAYRDKYGIN"
     gene            complement(1943..2905)
                     /locus_tag="PK856_04110"
     CDS             complement(1943..2905)
                     /locus_tag="PK856_04110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HRL01589.1"
                     /translation="MNHNEHILISGGTGFIGQHLIPVLLQQGHCISVYGREADKIQQL
                     FGDAVDAVSDLSQIKRPVNTIINLAGANIGESRWTEANKQRFIMSRVGTTQALLAWAK
                     QQEQPPQRLINGSAIGYYGVDTTEQWQQVCDESSPPQPVFMSQLCSQWEQAADGFKDL
                     GCDLVKLRLGVVLGQGGGVLDAMIKPIKLAKVGNVGSGRQPLVWVHINDVVAVVLWLM
                     AQPHWQSEVYNVVAPQITSQADFVNTACHVLKSRAPLRLPATLLRLMLGEQSDLVLNG
                     QKIAAKHLIEQGYVFQFAELNHALQDLLQTNEGNHANTVSVAKP"
     gene            complement(3141..>4045)
                     /locus_tag="PK856_04115"
     CDS             complement(3141..>4045)
                     /locus_tag="PK856_04115"
                     /inference="COORDINATES: protein motif:HMM:NF018448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HRL01590.1"
                     /translation="ITANKWWALIGLAILAFCIALQVGADWMTALGAALVQVSVSLMG
                     WIGGRIAIPRLFVVALQCMVCAVVAAGLVVTGLVASPGAVAIAVAWMLLVPQPQVINM
                     VIDAINTLQLAALARFASVAVAVGGILVGGAAVIHIGRQFVDKVPDPTLLSLPLPLAL
                     FFSLIGAMANAVANGGRMSLILPAGVVGLLTAICNQFLTRGLEMHTTWAAGISAVLLG
                     LVATFLARKLSYPASVLALMGMTAALLPGLTVYMGLATSFTGVSGMTEYLQAALVCVV
                     LGVGVALGFAIARLILRKPHWEAP"
     assembly_gap    4046..4105
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4106..4429)
                     /locus_tag="PK856_04120"
     CDS             complement(<4106..4429)
                     /locus_tag="PK856_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01591.1"
                     /translation="MHDLDGRRRLIEQEEVVAQLLAGLLNAGLSTSDAIALGRVAAVK
                     LGLDAVEFVDLGRSVLIQHGHPDGQVRTVSRPVGLDSINCEQLRALTLVCKQISAGEL
                     DIQTAP"
     gene            complement(4626..5078)
                     /locus_tag="PK856_04125"
     CDS             complement(4626..5078)
                     /locus_tag="PK856_04125"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HRL01592.1"
                     /translation="MTLTIFYDDSCPICRTEILGFYSVYPKDMNLISLSQGTDELKQH
                     NIQLEDALTLMHVIDEQGELHIGMDAIRVMYRRLGKPKTAWFTSLPVLNGFFNWLYPI
                     FARHRYKMPVWLLGINVKDVNDGCQDGYCQMPVAQKAAQLRKQGQKQE"
     gene            5387..>6008
                     /gene="lgt"
                     /locus_tag="PK856_04130"
     CDS             5387..>6008
                     /gene="lgt"
                     /locus_tag="PK856_04130"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRL01593.1"
                     /translation="MLMHPNFDPVALQLGPLAIRWYALSYIVAFALFIWLGRRRIKQG
                     NTVFTNDMLDDLLTYGVIGVILGGRVGYVLFYNFAQYMEHPLSIFKVWEGGMSFHGGF
                     LGVLLAMLVYAKRHKLTFFQVTDFIAPMVPAGLASGRVGNFINGELWGRVTDINAFWA
                     MGFKQARYEDEAMAPTNPQWLEWFNQYGVLPRHPSQLYQFALEGLLL"
BASE COUNT         1525 a         1529 c         1329 g         1565 t
ORIGIN      
        1 caaaggaaag tcttgttcgg gctcaatagc ggcctgtaag cgctgatttt gaaggtttaa
       61 tgcttcatga gcagaacatt cagccgcaat cacttgcgga tagctgactt gccagcggtc
      121 tagccacata aagtggccga tgctggcatc gcgcagcaac accaaacgca attcttcgcg
      181 ctcgctcagg ttcattacaa atcgtaatcc accaccaatg gtgcgtggtc tgagaatttt
      241 tcgtctttgt acacatgcgc ttcgcgcgcc aaggcatata ggcttggggt ggtcatgtgg
      301 taatcgatgc gccaacccac gtctttggca taggcttggc cgcggttgct ccaccaagtg
      361 tagccaggat tgtcaggata aagcttgcgc catgtgtcta cccaaccgac ttgttccaag
      421 agtttggtga gccaagcgcg ttcttcaggc aagaaacccg aatttttctg attgcccttc
      481 cagtttttca aatcgatgtt gttgtgggca atgttccagt cgccacacac gacgatgtgg
      541 cggccttctg ctagtaagcc ttccaacacg ggcaagaagg cgtctaaaaa ctcgtatttg
      601 taggtttggc gctcttcggc actggtgcct gaaggcaggt ataaagacac cacgctgagg
      661 tttttgtcat caaaatcggc gcggatatag cggccttcgt tgtcgaaagc ttctatgccc
      721 aaaccgtatt gaacacggtc aggcgtggct ttgctgtaaa tggccacgcc gctgtaacct
      781 gctttttggg cgcaatgcca cacgccgtgc atgccattcg gctttttgag cacatcggtt
      841 aatttgtctt cttgggcttt caattcttga acacaaacga tatcggcacc agaagcctgt
      901 atgtattcaa taaaaccttt agatgcggcg gagcgaatgc cattgacgtt ggcgctgatg
      961 attctaaaca cgggcttcct cgggtacaat acgcaaaaat tgaacggctg cggccgtttt
     1021 gatgcctcat tgtaccgatt taaggattat tcatgtcaga ttttcgtcaa cagtttctca
     1081 aatttgccct cgaacaagag gttttgaaat ttggtgaatt catcaccaaa gccggccgcc
     1141 cttctccgta tttcttcaat gccggtctgt tcaacgatgg caatggcatc ttgcaattgt
     1201 cgcgctttta cgccgatgcc atcattgcca gtggtgtgga atttgacatg ttgtttggtc
     1261 ctgcttacaa aggcatcatt ttggcagcgg ccaccagcat ggctttggct gaaaaaggcg
     1321 tgaacgtgcc catcgcttac aaccgcaaag aagccaaaga ccacggcgaa ggcggcgtgt
     1381 tggtgggcgc gcctgtacaa ggccgtgtgt taatcattga tgacgtgatt tctgctggca
     1441 cttcggtgcg tgaatcggtg aaattgatcg aagccgaagg cgcaacccct gctggcgtgg
     1501 tgatagcctt agaccgcatg gaaaaaggca caggcgaaac ctcagcagta caagaagtag
     1561 aacaacaata cggcttgcct gtggtgtcta tcgccaattt gcatgactta ttggcgatgt
     1621 tggctgacga ctcaactttg cagacttact tggtggctgt gcaagcgtat cgtgataaat
     1681 atggtattaa ttgatttttt aatataattt tgaccttaaa gccgactttt gtcggctttg
     1741 ttgtttgaat tttattaaaa agagataata tcaaatgcat tatgttaatt aaggtatgaa
     1801 taaaccttga tgctcaaaca tattggctac taatcaatgg gaattcagtt gtttatgcgg
     1861 tgtgaagaac atgcagatat aagtaagcgg cctgtaaacc aatagagagg tttacaggcc
     1921 gcttattaga aacattctaa agctatggtt tggctacact caccgtattg gcatgattgc
     1981 cttcatttgt ttgcaataag tcttgtaacg catggtttaa ctcagcaaat tgaaacacat
     2041 agccctgctc gattaagtgc ttggcggcga ttttttggcc gtttaacacc aaatcgcttt
     2101 gttcacccaa catgaggcgc aacagcgtgg caggcaagcg cagcggtgca cgacttttca
     2161 acacatggca ggctgtattg acaaaatcgg cttgggaagt aatttgcggc gccaccacat
     2221 tgtaaacctc gctttgccag tgcggctgtg ccatcagcca caacaccacc gccaccacat
     2281 cattaatgtg cacccacacc aaaggttgtc gacctgagcc aacattaccc actttggcca
     2341 atttgatagg cttaatcatg gcatccagca caccaccgcc ctgccccaac accacgccca
     2401 atctcaattt cacaaggtca cagcccaagt ctttaaaccc atcagcggcc tgttcccatt
     2461 ggctgcacaa ttgcgacatg aacacaggtt gtggcgggct cgattcatcg cacacttgtt
     2521 gccattgctc cgtggtgtct acgccgtagt agccgatggc cgaaccatta atcaaacgct
     2581 gcggcggctg ttcttgctgt ttggcccatg ccaacaaggc ttgcgtcgtg cccactcggc
     2641 tcatgataaa gcgctgtttg ttagcttctg tccagcggct ttcgccgatg ttggcacctg
     2701 ctagattgat gatggtgttt acaggccgct taatctggct taaatcactc accgcatcca
     2761 cagcatcacc aaacaattgc tggattttat ccgcttcacg cccatacacg ctgatgcaat
     2821 ggccttgttg cagcagcaca gggattaaat gttggccaat gaaacctgtg ccgccgctga
     2881 ttaaaatgtg ttcattgtga ttcatggtcg tgactcctga ttttggctat attttgctgc
     2941 acaggcatat tgcgacaagc gtatgcagcc aattaaaaaa ccattcaaaa caggcaagct
     3001 ggtaaccatt cgatttttag tttgcccaaa catcgctaca tctcacgtat ggcacccatg
     3061 ccaatgtgta atacactatt ctcatcaatg tcttatcagg gttatggtat cttccgatgg
     3121 tcccaagtct gacagctctc ttacggagcc tcccaatgcg gctttctcaa aatcaggcga
     3181 gctatggcaa aacccagtgc cactcctact cctaacacaa cgcaaaccaa agccgcttgt
     3241 aagtactcag tcataccgct aacgcctgta aacgaagtgg ccagccccat gtaaacagtc
     3301 aagcctggca agagtgctgc tgtcatgccc attaaagcca gcaccgaggc tgggtaggac
     3361 aatttacgcg ctagaaacgt ggcaacaagt cccagcaata ccgcagaaat acctgcggcc
     3421 caggtcgtgt gcatttccaa tccacgggtc agaaactggt tgcaaatcgc agtcaacagc
     3481 cccaccactc ctgcaggcaa tatcaagctc attcgaccgc cgtttgcgac agcattggcc
     3541 attgccccaa tcagcgaaaa aaataatgcc aagggcagag gcagcgacaa cagggtcggg
     3601 tcgggtacct tatccacaaa ctgacgacct atgtggatta ctgccgcgcc acctaccaaa
     3661 ataccaccca cggcaaccgc aacagatgca aaacgcgcta gcgcggccag ttgcaatgta
     3721 ttgatggcat caatcaccat attaatcact tgcggttgtg gcaccaaaag catccacgcc
     3781 accgcaatgg ctaccgcgcc aggagaagcc accaacccag tcacaaccaa gcctgcagcc
     3841 accacagcgc acaccatgca ttgcagcgcc acaacgaaca gccgaggaat ggcgatgcgc
     3901 ccaccaatcc accccatcaa agacaccgac acttgaacca gcgcggcacc caacgccgtc
     3961 atccaatctg cccccacttg taaagcaatg cagaacgcca aaatagccaa cccaatcaac
     4021 gcccaccact tattggcggt aattgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnngggtg cagtctggat gtcaagctca cccgcactaa
     4141 tctgtttaca caccagagtg agtgcacgca gttgttcgca gttaatgcta tcgagcccca
     4201 caggtcgaga gacagtgcgg acctgcccat caggatgacc atgctgaatc agaacagaac
     4261 gcccaagatc cacaaattcc actgcatcta ggcctagctt taccgctgct actcgcccca
     4321 aagcaattgc atccgatgtt gataatcctg catttagcaa accagcaagc aactgagcaa
     4381 ccacttcctc ttgttcaatc agtcgtcttc gtccatccaa atcgtgcatc ctaaagtcct
     4441 cttcatttct ctaaacattt catcatctca cattttagag tccaacaagg cttcacgtat
     4501 gcattctaga gtttcaatac gattttgttt atcttcatca aaactgccgc catcaaatct
     4561 tcactttcac tgctacactg tcaaaccttt aaatgaggtc agtgccgctg cgaatgagaa
     4621 tgtgttcatt cttgcttttg gccttgtttc cttaactgtg cggctttctg tgccacaggc
     4681 atttggcaat agccatcttg acacccgtcg ttcacatctt tcacattgat gcctaacaac
     4741 cacacaggca ttttgtagcg atgacgtgcg aatatgggat acaaccaatt aaagaagcca
     4801 tttaagacgg gcaaactggt gaaccatgcg gttttgggtt tacccaaacg acggtacatc
     4861 acccgtatcg catccatacc tatgtgcaac tcgccttgtt catcaattac atgcattagg
     4921 gttaaagcat cttccaattg gatgttgtgc tgcttcaact catcagtacc ttgcgacaag
     4981 gagattaagt tcatgtcttt tggataaaca ctgtaaaaac ccagtatttc agtacgacaa
     5041 atgggacaag agtcatcata aaatatcgtt aaagtcatca tcatctcctc ggctgtgcaa
     5101 ctatgtttgg ctggttcaag tagaattctg ctcataacct tgtaatgcct ggctcacact
     5161 caacacatac accaaaaaat catcaatttt aaacattgcc tttaaaccac tgacacagca
     5221 gtattaattg gtatgcttat tccacttcat tgatagtcat attattaaaa tccgtaattt
     5281 ctgtcaatac agaattttat aatattttta tccaacataa tttagcaatg atgtttgcta
     5341 gaatgtgcgc cactgttcac atttaagaca agagagtttc gccatcatgt tgatgcaccc
     5401 aaactttgat cccgtggcct tacaattggg gccattggcc atccgttggt atgccttgag
     5461 ctatatcgtt gcttttgcct tgtttatctg gctgggccgc cgtcgcatca aacaaggcaa
     5521 tacagtgttt accaatgaca tgctggacga tttgctcacc tatggcgtga ttggggtgat
     5581 tttgggtggc cgtgtgggct atgtgttgtt ttacaacttt gcccaataca tggagcatcc
     5641 gctgtctatc ttcaaagtgt gggaaggcgg catgtcgttt catggtggtt tcttaggggt
     5701 gttgctggcg atgttggtgt atgccaaacg ccataagctt actttcttcc aagtcaccga
     5761 ctttattgcg cccatggtgc ctgcaggttt ggcttcaggc cgcgttggta atttcatcaa
     5821 tggcgaattg tggggacgtg tcaccgacat caatgcgttt tgggcgatgg gtttcaagca
     5881 agcgcgttat gaagacgaag caatggcgcc caccaaccca cagtggctgg aatggtttaa
     5941 ccaatacggc gtattgcccc gtcatccgtc acagttatac caatttgctt tagaaggctt
     6001 gttgttgt
//
LOCUS       DAOMVK010000082         5946 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_3599_length_5946_cov_3.225089, whole genome shotgun sequence.
ACCESSION   DAOMVK010000082 DAOMVK010000000
VERSION     DAOMVK010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 5946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5946
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3599_length_5946_cov_3.225089"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="PK856_04135"
     CDS             <1..817
                     /locus_tag="PK856_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRL01594.1"
                     /translation="DIDFLEAADAKGKLQHIRKQLDGVMAQAQQGAILREGMQVVLVG
                     APNVGKSSLLNALAGDDIAIVTDIAGTTRDTVREMITVDGVPMHIIDTAGLRDTDDVV
                     EKIGIERSHKAVQQADVALILIDPREGVNARTQEIVDGLPEQLPCLEIHNKSDLLDAT
                     VLQTQQYIARASDVMTLSAKKGDGLAQLKQALLKQVGWQGEAEGLFLARTRHLHALQA
                     AAQELAFAADCGDMIDLLAEHLRLAQVCLNEITGEFTADDLLGVIFSRFCIGK"
     gene            complement(1355..1882)
                     /locus_tag="PK856_04140"
     CDS             complement(1355..1882)
                     /locus_tag="PK856_04140"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HRL01595.1"
                     /translation="MQSHQTFGVGMGKWLVGILMSYSLAVYAQEGVADAETTDIDASY
                     NLQASDASVGLITTYISKNRTEAPQPTLVNPSSASQYTSFGIGPSVRLNEYVTAYGVI
                     GAAADARSNFDTAMPVQALTLDNDARMYGVGMQIRPNQDWQIDMKYQNANLNSGADAT
                     RQVNYFNVGVGYKFW"
     gene            complement(2031..2501)
                     /locus_tag="PK856_04145"
     CDS             complement(2031..2501)
                     /locus_tag="PK856_04145"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRL01596.1"
                     /translation="MNAVNIRLARPDEAELLLQIHHASVQGLCAGQYDAEQIAHWFDG
                     RDASIYTQGIVQQRLWLACAPDPVGFIEVDEHSIDKLFVLPQAAGLGVGKLLLLHGLA
                     FLQAQGQREIEIDATLTAAPFYQKHGFEPMAQTLSKHGAEDARLPVVRMRWSAA"
     gene            2680..3090
                     /locus_tag="PK856_04150"
     CDS             2680..3090
                     /locus_tag="PK856_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01597.1"
                     /translation="MNTWYGYFAPFPVDDFQPLQTLTETQTWLGNASTIHSQDIAAQP
                     DLYFDATMEVLVEHKTVHHIAHFNQHYAEALVEAEKLGYEVEQPRHEPYVFWLPSPQG
                     YCLCGFIFKNDNDGDTFIVSPVALPFLHATETSE"
     gene            complement(3155..3856)
                     /locus_tag="PK856_04155"
     CDS             complement(3155..3856)
                     /locus_tag="PK856_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01598.1"
                     /translation="MKAWWMLLAVLPVGALAAPAVQDFVPQGWKLTQQAAGDLNGDGR
                     ADKVLVLQQQNTANWRDNEALGAPRLNLNPRTLLVLWNTPQGYREAVRNTTLLPSENS
                     EDTPCLADPLDKGAISIRKQVLVVDLHYWLSCGSYGVNHMQYKFRHQQGAWPLIGLDV
                     NSSARNMDDTEQSSYNFATGRALYTGVKTQITASGDSKVVRLSKPSRISNPRVDLRQS
                     GVEAKVGQWLERNAL"
     gene            complement(3896..4648)
                     /locus_tag="PK856_04160"
     CDS             complement(3896..4648)
                     /locus_tag="PK856_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HRL01599.1"
                     /translation="MSANCVVIQAALPLECEYLVGLLANVQQETRGGWVFWRGEYQGM
                     DLIVSKTRMGLSNAAAATAIAIERYQPRAIINQGTSGGHNPDLNVFDIVLGLETTNFG
                     MIKTPFAEKGAGSNSLHWHEAFDMLPDDESDPEPIRKRYYPADAALLAAARAVKQTYA
                     QGKVVEGVIGSADCWNNELDRIAFLREMYGTEVEEMEAASVAQIAQQYGVPMMAIRVV
                     SNNITNGGAYNPDTGKACQDFVLQVVEQYLRT"
     gene            complement(4712..5896)
                     /locus_tag="PK856_04165"
     CDS             complement(4712..5896)
                     /locus_tag="PK856_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRL01600.1"
                     /translation="MSESQLSREISVARRMHDIEPFRVMALLDRAKALQAAGRDIIHM
                     EIGEPDFTTAAPIVAAAEQALQAGKTAYTQALGIPELRQAIAAWYQKQYGLDIAPARI
                     VVTPGGSGALLLLSALLINPEEELLLSDPSYPCNRHFLRLIEGRARLIETTPETRYQL
                     NADLVREHWQPQTRGALVASPANPTGEMLSREELRDLADACREKNGVLLVDEIYHGLT
                     YGEDAVSVLEVADDAFVLNSFSKYFGMTGWRIGWLVAPEALVPQIEKLAQNLYISVTT
                     VSQYAALAAFSDEAVAIHEARRAAFAERRDYLVGALRELGFTVPHVPAGAFYVYADVS
                     AFTQNSRQWCLDLLEQEGVAVTPGEDFGRYRCDEHVRFAYTDDLPRLQEAVARIQRFI
                     AS"
BASE COUNT         1469 a         1687 c         1416 g         1374 t
ORIGIN      
        1 agacattgat tttctcgaag ccgccgatgc caaaggcaaa ttgcaacaca tccgcaagca
       61 gctcgatggc gtgatggcgc aagcgcagca aggcgcaata ttgcgcgaag gcatgcaagt
      121 ggtgttggtg ggtgcgccca atgtgggcaa atccagcttg ctcaatgctt tggcaggcga
      181 tgacattgcg atagtcaccg acatcgccgg caccacgcgc gataccgtgc gcgaaatgat
      241 taccgttgat ggcgtgccca tgcacatcat cgacaccgca ggcttgcgtg acaccgacga
      301 tgtagtagaa aaaatcggca tagagcgcag ccacaaagcg gtgcaacaag ccgatgtggc
      361 cttaatcctc atcgacccac gcgaaggcgt gaatgcgcgc acccaagaaa tcgttgatgg
      421 tttgcctgag caattgccgt gtttggaaat tcataacaag agcgatttgc tcgatgccac
      481 cgtgttgcaa acccagcaat acattgcccg cgccagcgat gtgatgactt tgtcggcgaa
      541 aaaaggcgat ggcttggccc agcttaaaca ggctttgctc aaacaagtgg gctggcaagg
      601 tgaagccgaa ggtttgtttt tggcgcgcac ccgccatttg catgccttac aggccgctgc
      661 acaagagctg gcgtttgctg ccgattgcgg tgacatgatt gatttattgg ccgaacattt
      721 gcgcttggca caagtgtgtt tgaatgaaat cacgggtgaa tttaccgccg atgatttgct
      781 cggtgtgatt ttcagccgct tttgcatagg caaataagct tcgttcccca ttcaaaaaaa
      841 acggcgtttc gtagcaacga aacgccacaa gactcttcaa ttgaagcaga aggagaacgt
      901 aaacagacat ggtatgcttg cctgtgtttg ttatcctaag gcattggctt gcaaaagaat
      961 gcttgctttg gctcttagca ccaaaagcaa ttagcccaag caagcactca actggcgttt
     1021 attcactgcg cttttgaatc cacataaaca gcgaattgca ttaggggctc ttcatcgccc
     1081 gagttgagta tggaaaaccc tcttccacat ctgctacaac gccattcatt aatgcgtcaa
     1141 caccaccact gaactcacct taaaacccaa tattgaagcg gcctgtaaac acaataacaa
     1201 ggtttacagg ccgctttgat actatttttt aatgcctatg cgtacagttc tcatcattca
     1261 agcatcaaga taaacggctg gttcattttc agccaaacgc catctcaatg ttaccaactc
     1321 tctgccacca tcaattgcgt ttgtgttgca gcagtcacca aaatttgtag cccacaccaa
     1381 cattgaaata attgacttgg cgcgtggcat ccgcgccgct gttcaggttg gcattttggt
     1441 atttcatgtc gatttgccaa tcttgattgg ggcgaatttg catgcccacg ccatacatgc
     1501 gcgcatcgtt gtccaaagtc agcgcttgca ctggcatggc cgtatcaaaa ttgctgcggg
     1561 catcggcagc tgcgccgatg acaccgtatg cagtgacgta ctcgtttaat ctgacactgg
     1621 ggcctatgcc aaaagaagtg tattgactgg cagagctggg attgaccaaa gtaggctgcg
     1681 gggcttcggt gcggttttta ctgatgtaag tggtgatgag gccaacggat gcgtctgagg
     1741 cttggagatt gtagctggca tcgatgtcgg tggtttcagc gtccgccacc ccctcttgcg
     1801 catacacagc aagcgagtaa ctcatcaata tccctaccaa ccatttgccc atgcccaccc
     1861 caaatgtttg atgcgattgc acaaaatctt aagataacgg catttactct aatcggtcaa
     1921 cgaaaatcat tgcaggcgcg gtttacaggc cgctgtttcc agccattaat cgcgccaatt
     1981 cagacccaaa taccgcgata catttttcca tgcttacaaa cccaatgcac ttatgccgcc
     2041 gaccaacgca tgcgcaccac aggcaaacgc gcatcctcag caccgtgttt ggacagtgtt
     2101 tgcgccatag gctcaaaacc atgcttttgg taaaacggtg cggcggtcaa tgtggcatca
     2161 atctcaattt cgcgttgccc ttgagcttgc aaaaatgcca agccatgtag caacaacagc
     2221 ttgcccacac ccaaacccgc cgcctgtggc aacacaaaca atttgtctat gctgtgttcg
     2281 tccacctcaa taaacccaac gggatcgggc gcacacgcca accacagacg ctgttgcaca
     2341 atgccttggg tgtaaatgct tgcatctctc ccatcaaacc aatgggcgat ttgctcggca
     2401 tcgtattggc ctgcacacaa tccttgcacg gaagcatggt gtatttgcaa caacaattcg
     2461 gcctcatcgg gccgagccaa acggatgttc acagcgttca tacacctact ccataacaaa
     2521 ttgatgacaa atacccacaa aaattcaaac acctgacccg catcaaccga tgcagcatat
     2581 caatgcccaa ttggcataat taatcattgg tacatattga cgcctgctca cttcatcacc
     2641 acaatatccg cacaacaaca aacacaataa ggggaagcga tgaacacttg gtatggctat
     2701 tttgccccgt ttccagtgga tgattttcag ccattgcaaa cgttaaccga aacccaaacc
     2761 tggctgggta atgcctctac catccattcg caagacattg cggcgcagcc cgatttgtat
     2821 ttcgatgcca ccatggaagt gctggtagag cacaaaactg tacaccacat cgcccacttt
     2881 aaccaacact atgccgaagc gttggtggag gctgaaaaat tgggctatga agtggaacaa
     2941 ccgcggcacg aaccttacgt gttttggttg cccagcccac aaggttattg cctgtgtgga
     3001 tttattttta agaacgacaa tgacggcgac acattcatcg tgtcgcccgt ggccttgccg
     3061 tttttacacg ccaccgaaac ctcagaataa gccttaagca aacacacaag cggcctgtaa
     3121 atatttacag gccgcttgca tgagccatca gtcctcacaa agcatttcgc tctaaccatt
     3181 gccccacctt ggcttcaaca ccgctttgtc gcaaatccac acgcggattg ctgatgcgac
     3241 tgggtttgct tagtcgcacg actttgctgt cgccgctggc ggtgatttgg gttttcacgc
     3301 ctgtgtacag tgctctgccc gtggcaaaat tgtaactgga ttgctcggtg tcatccatgt
     3361 tgcgcgcgct gctgttcaca tccaagccta tcaaaggcca agcgccttgc tggtggcgaa
     3421 atttatactg catgtggttc acgccgtaag aaccgcaact gagccaataa tgcaaatcca
     3481 ccaccagtac ttgcttgcgt atgctgatgg cgcctttgtc caaagggtcg gccaaacatg
     3541 gcgtgtcttc actgttttca cttggcagca aagtggtatt gcgcaccgct tcgcgatagc
     3601 cttgcggcgt gttccacaac accagcagcg tgcgtggatt cagattcaaa cgcggcgcac
     3661 ccaaagcctc attgtcccgc caattggctg tattttgctg ctgcaacacc aataccttgt
     3721 cggcgcggcc atcgccattc aaatcgccgg ctgcctgttg cgtaagcttc cagccttgtg
     3781 gcacaaagtc ttgcaccgca ggcgcggcaa gcgcgccgac aggcaacacc gccaataaca
     3841 tccaccacgc tttcatactg acctccctca tgtttacagg ccgcttatgc tcggcttaag
     3901 tccgcaaata ttgctccacc acttgcagca caaaatcttg gcaagccttg cctgtgtctg
     3961 gattgtaagc gccgccattg gtgatgttgt tcgacaccac ccgtatcgcc atcataggca
     4021 cgccgtattg ctgcgcgatt tgtgccaccg aagccgcttc catttcttcc acttccgtac
     4081 catacatctc gcgtaaaaac gcgatgcggt ccaattcatt gttccaacaa tcagccgagc
     4141 ctatcacgcc ctccaccact ttgccttggg catacgtctg cttcaccgcc cgcgccgccg
     4201 cgagcaatgc ggcatcggca gggtaataac gtttgcgtat cggctcgggg tcggattcgt
     4261 catcgggcaa catgtcaaac gcctcatgcc aatgcaagct gttgctgcct gctcctttct
     4321 cggcaaatgg ggttttgatc atgccaaaat tcgtggtttc caaacctaac acaatgtcga
     4381 acacattcaa atcaggattg tgcccacccg aagtgccttg gttgatgatg gcgcgtggct
     4441 gatagcgctc tatggcaatg gcggtggccg cggcggcatt gctcaagccc atgcgcgttt
     4501 tcgacacgat gaggtccatg ccttgatatt cgccacgcca aaacacccaa ccgccacggg
     4561 tttcttgctg cacattcgcg agcaagccca ccaaatactc acattccaac ggtaaagccg
     4621 cttggatgac cacacagttt gcagacataa cattcctcta taagcggcct gtaaacagtt
     4681 ttacaggctt acaggccgca tcaatccatg attaagaagc aataaaacgc tggatgcgcg
     4741 ccacggcttc ttgcaagcgc ggcaaatcgt cggtataggc aaagcgcaca tgctcatcgc
     4801 aacggtagcg accaaaatcc tcacctggcg taaccgccac gccttcttgc tcaagcaaat
     4861 ccaaacacca ttggcggcta ttttgcgtaa acgccgacac atcggcatac acataaaacg
     4921 cgcccgcagg cacgtgtggc acggtaaagc ccagctcacg caacgccccc accaaataat
     4981 cgcggcgctc ggcaaaggcc gcacggcgcg cctcgtgtat cgctaccgcc tcgtctgaaa
     5041 aagccgccaa ggccgcgtat tgcgacaccg tggtcaccga gatgtacagg ttttgcgcca
     5101 atttttcgat ttgcggcacc aaggcttctg gcgcaaccaa ccaaccgatg cgccagcccg
     5161 tcatgccaaa gtatttggaa aaactgttga gcacaaacgc atcatcggcc acttccaaca
     5221 cacttaccgc gtcttcgcca tacgtcagac catggtaaat ctcgtccacc aacaacacgc
     5281 cgtttttctc acgacaggca tcggccaaat cgcgcaattc ttcacggctg agcatctcac
     5341 ccgtagggtt ggcaggcgag gccaccaagg cgccgcgcgt ttgcggctgc caatgttcgc
     5401 gcaccaaatc ggcattgagc tgatagcgcg tttcaggcgt ggtttcaatc aagcgcgctc
     5461 ggccttcgat gaggcgcaaa aaatggcggt tgcacgggta gctcgggtcg gacagcaaca
     5521 attcttcttc gggattgatg agcaaagccg acaacaacag caaagcgcca gaaccaccag
     5581 gcgtgaccac aatgcgggca ggcgcaatgt ccaaaccgta ttgcttttga taccaagccg
     5641 cgatggcttg gcgcaattcg ggtatgccta aggcttgggt atacgcggtt tttcccgctt
     5701 gcaaggcttg ctcggcggct gccactatcg gcgcagcggt ggtgaaatct ggctcgccta
     5761 tctccatgtg gatgatgtca cgacctgcgg cctgtaaggc tttggcgcgg tctagcaagg
     5821 ccatcacacg gaatggctca atgtcgtgca tgcgacgggc aacagaaatt tcacggctca
     5881 gttgggattc agacatggcg cttcacattc aaaaatcgga tgacaagagt atagcatcgc
     5941 tccttc
//
LOCUS       DAOMVK010000083         5908 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_3629_length_5908_cov_3.411926, whole genome shotgun sequence.
ACCESSION   DAOMVK010000083 DAOMVK010000000
VERSION     DAOMVK010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 5908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5908
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3629_length_5908_cov_3.411926"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..1164
                     /locus_tag="PK856_04170"
     CDS             259..1164
                     /locus_tag="PK856_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRL01601.1"
                     /translation="MSQILLALFPSIALIASGYVFQQRRFFATEFWNGAEKLNYYVFF
                     PALLFSSLALAQADWQHLSDVIWTMLSVIAVVMLLMYGLRAKRHVLPARFGVYVQSAV
                     RFNTYIGLAIVSALAAGNGSQMLAVILAVCIPLVNVISIIALLPKEQLRLGNVLVSVL
                     KNPLVSSCVAGIAVNLLDIPLWLGLQQLLKLLATCSLTMGLLCVGAALQFTQVKNKLS
                     LVMLNSVWRLLCIPMLAWGMCQLFGLNEVATQVLVIFFGLPTASASYILTKVLKGDAP
                     LMASIISMQTLMSMVTLPLLIVLTA"
     gene            1242..1778
                     /locus_tag="PK856_04175"
     CDS             1242..1778
                     /locus_tag="PK856_04175"
                     /inference="COORDINATES: protein motif:HMM:NF019599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcnI family protein"
                     /protein_id="HRL01602.1"
                     /translation="MKLTHTLGLASALLAAQTQAHVSMLAPENIAQGQSFKAVVAIPH
                     GCDGSATTAVTIHMPIGIIAVKPQPKAAWRISKTEQTYAQPYDYYGRALTSGVKSLTW
                     HGRLADDEYDEFVFQAYASAIPSGTGKVFFKLEQQCEHGKLMWTDVSGKKMTGHSAPE
                     LTAPSVPVSRSDAAVHHH"
     gene            complement(1901..2842)
                     /locus_tag="PK856_04180"
     CDS             complement(1901..2842)
                     /locus_tag="PK856_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRL01603.1"
                     /translation="MLNVLCRKPTWYAGLCLSITLGFALPAHADDLDVIGEFIQEQNL
                     AKPEATPQAQLQEFTSDVQELGPALNSQSALIMDANSGKPIYQKNVNSVRSIASISKL
                     MSAMVVLDAGLDKNERVTITDAEIDRLKGTGSRLSVGTTLSREELLHIGLMSSENRAI
                     HALARTYPGGMPAFVSAMNRKAQQLGMNNSRFVEPTGLSPQNVSTANDLAKMVQAAAQ
                     YPQIRYFSTHNNATVYTSNGKAQQYKNSNALVREGAWPIIVQKTGYIREAGRSMVVET
                     QVQGRPVIMVLLNSPSSATRVNDARSLGNWLQWQRPA"
     gene            3106..4299
                     /locus_tag="PK856_04185"
     CDS             3106..4299
                     /locus_tag="PK856_04185"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinyldiaminopimelate
                     transaminase"
                     /protein_id="HRL01604.1"
                     /translation="MENYLTPNFAFAPVIPDHAQGSHVWDTEGREYLDLAGGIAVNAL
                     GHCHPALQAALTEQAAKLWHISNIYTTAPAQQLAQRLVENTFADKVFFCNSGAEANEA
                     AFKLARKYAFDHFGPEKNEIIACVNGFHGRTLFTVSVGGQPKYQQGFAPLPAGIKHIP
                     FNDVDALAAAISDKTCAVVIEPVQGESGVLPASKDFLQAARQLCDEHNALLVLDEVQT
                     GMGRTGKLFAYEHFGVVPDIVSSAKALGAGFPIGAMLTTDKVAPSFAPGTHGSTFGGN
                     PLAAAVGNAAFDIIHQAETLAHVRAQGERLQVELRKIGETHGVFAEVRGLGLLIGAVL
                     TDAYHGKAGEFVKVALTQGLMILNAGTNVVRFAPSLLLSDADLQAAVVKLNATAAQFV
                     AEQGV"
     gene            4299..4991
                     /locus_tag="PK856_04190"
     CDS             4299..4991
                     /locus_tag="PK856_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HRL01605.1"
                     /translation="MSAKAKVVVLTGAGISAESGLLTFRDAGGLWEGHKVEEVATPEA
                     FARNPQLVLNFYNQRRAQLQQVAPNPAHLALVDLEQAYDVHIITQNVDDLHERAGSSH
                     ILHLHGELTKVRSSVNEDYVVAWDGDQQLDDKDPQGYPMRPFIVWFGEAVPLIENAVS
                     IIANADVVLVVGTSMQVYPAASLLDYAPLDIPVYYIDPAAQAMESAVHIRAEVASVGV
                     PAVVKELLAAAS"
     gene            4999..5670
                     /locus_tag="PK856_04195"
     CDS             4999..5670
                     /locus_tag="PK856_04195"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HRL01606.1"
                     /translation="MSWLKPTTWLAVLCRQVAAYRFWLALVLALFGTVLLHGLWLWLE
                     ALTAAQEHFFGRGLGIVATWVAFGLQFAWPVLILTFGVLGVWQRWRGDSVFRRMQRQS
                     SQGFEQLSWQDFEALIAELFRRHGFEVEEQGGDHADGGIDVIVKRDECTYLVQCKHWQ
                     AQRVGVKVVRELYGVIAAEGVDGGYVITSGHFTPDAVDFAQGIAMQLINGRQLMALLA
                     DELEL"
BASE COUNT         1364 a         1244 c         1633 g         1667 t
ORIGIN      
        1 aacggattga aaatccgtgt gtcggcggtt cgatcccgcc tctggccacc atttgattcc
       61 tctgtttcaa ttcattttct ccccatttca ttgttgctat agtcataagc ggcctgtaag
      121 ccatttatag tctgaaaata cccattcatt taaattagcc tcattatttg attacacgac
      181 tgtgctaagc ttgtggagtg aatgacatgc aaatgaacac agtgtttggc atggcgaaaa
      241 tttcagatga gtacagcgat gtcgcaaatt ttattggcct tattcccgtc cattgcctta
      301 attgccagtg gttatgtgtt tcagcagcgg cgtttttttg cgactgaatt ttggaatggt
      361 gctgaaaaac tcaattatta cgtctttttc cccgctttat tgttttcttc tttggctttg
      421 gcgcaagcag attggcagca tttgtccgat gtgatttgga cgatgttgag cgtgattgct
      481 gtggtgatgt tgctgatgta tgggctgcgc gccaagcgcc atgtcctgcc tgcacgtttt
      541 ggggtgtatg tgcaaagtgc tgtgcgtttc aatacctaca taggtttggc gattgtttcg
      601 gcattggcag caggcaatgg gtcgcaaatg ctggctgtaa tcttggcggt gtgcatccct
      661 ttggtgaatg tcatctccat cattgcattg ttgcccaaag agcaattgcg tttgggcaat
      721 gtgctggtgt ctgtattaaa aaatcccttg gtcagctcgt gtgtggcggg cattgccgtc
      781 aatttgttgg acatcccttt gtggttgggt ttgcagcaat tgttaaaact attggccact
      841 tgcagtttga ccatgggttt gttgtgtgtg ggtgcggctt tgcaattcac tcaagtcaaa
      901 aacaaactgt cattggtgat gctgaacagc gtttggcgcc tgttgtgcat ccccatgttg
      961 gcttggggca tgtgtcagct gtttgggttg aatgaggtgg ctacacaggt attggtgatt
     1021 ttctttggtt tgcccactgc atcggcttct tatatcttga ccaaggtttt gaagggggac
     1081 gcgccattga tggccagcat catcagcatg caaactttga tgagcatggt gacactacct
     1141 ttgttgatag tgttgacggc ataaggctct gcgacaattt gtcgcagtgt gtgttagagc
     1201 aaataccgtc ataatggcgt accaaaaaaa gaaacatcat catgaaactc acacacacat
     1261 tgggcttggc cagcgcacta ctggcggccc aaactcaggc gcatgtcagc atgctggcgc
     1321 ctgaaaacat cgctcaagga cagtcgttta aagcggttgt ggcgattcca catggttgtg
     1381 atggcagtgc caccaccgcc gtcaccattc atatgcccat tggtattatt gcggtcaagc
     1441 cgcaaccaaa ggcagcttgg cgcatcagca aaaccgaaca aacctatgcc caaccgtacg
     1501 attattatgg tagggcgctc acaagcgggg tgaaaagcct gacttggcac gggcgtttgg
     1561 ccgatgatga gtacgacgag tttgttttcc aagcctatgc ctcagccata ccttcgggaa
     1621 caggcaaggt gtttttcaag ctggagcaac agtgtgaaca cggcaagctg atgtggaccg
     1681 atgtcagtgg caagaaaatg acaggacatt ccgctcccga attgactgcg ccctctgtgc
     1741 cagtgagtcg cagcgatgct gcagtacatc accattaata aatttaagcg gcctgtaaca
     1801 ggcggtttaa cctcacagga ccaaccgtaa ccatgaatgc ggcctgtaaa gcaaaagcca
     1861 tctctttcga gatggctttt ttgattgcgg tgtccgttga ttaagcagga cgttgccatt
     1921 gtagccaatt gcccaagctg cgcgcatcgt tcacacgtgt ggctgagctt ggtgaattga
     1981 gcaagaccat gatgacaggg cgaccttgta cttgggtttc taccaccata gaacgacccg
     2041 cttcacggat gtaaccggtt ttttgcacaa taatcggcca agcgccttca cgtaccaaag
     2101 cgttggagtt tttgtattgc tgagctttgc cattgctggt gtaaacagtg gcattgttat
     2161 gggttgagaa atagcgaatt tgtgggtatt gcgcagcagc ttgtaccatt ttcgccaagt
     2221 cgtttgcggt ggaaacgttt tgtgggctca aaccagttgg ttcaacaaaa cggctgttgt
     2281 tcatgcccaa ttgttgtgct ttgcggttca ttgcggacac aaaagcaggc atgccaccag
     2341 ggtaagtacg ggctaaggca tgaatggcgc ggttttcact gctcattaag ccaatgtgca
     2401 acaattcttc gcggctcaat gtggtgccca cagacaagcg gctgcccgtg cctttcaagc
     2461 gatcaatttc tgcatcggta atcgtgacgc gttcattttt atccaagccc gcatccaaaa
     2521 ccaccatggc gctcatcaac ttactgatgg aagcaataga gcgcacactg ttaacatttt
     2581 tttggtaaat cggcttgcca gaattggcat ccataatcaa ggccgattga gagtttaacg
     2641 cagggcccaa ttcttggacg tcactggtga actcttgcag ctgtgcttga ggagtggcct
     2701 caggtttggc caagttctgt tcttggatga attcgcctat cacgtcgagg tcatcggcgt
     2761 gtgcaggcaa agcaaaacca agcgtgatgc tcaggcacaa accagcatac caagtgggtt
     2821 tgcggcacaa cacgtttaac ataagcgatc ctttttgcgt ttagaattgt tatttgtgga
     2881 cagacggtta taaatcatta tcggcggtat tgtaccgagc tgtattcagg ctaagtgtat
     2941 atttatgtaa atcaaacaat atttacaaag ccaaaatccg tatctcggca tcattttttg
     3001 cttgaatctg gttttaattg tgttacactt ttcaaaatgc ctgcatggtc aaataattca
     3061 catgcgccta caatacggct tttgctcagc acagacgatc attcaatgga aaactactta
     3121 acacccaatt ttgcttttgc acctgttatt cctgaccatg ctcaaggcag tcacgtttgg
     3181 gatactgaag gacgtgaata ccttgatttg gctggaggga ttgcagtcaa tgctttgggg
     3241 cattgtcacc cagcattaca ggccgcttta actgagcaag ctgccaagct gtggcacatc
     3301 tccaatattt atactaccgc acctgcgcag caattggcgc agcgattggt agaaaataca
     3361 tttgccgata aagtgttctt ctgcaattct ggtgccgaag cgaatgaagc cgcgtttaaa
     3421 ttggccagaa aatatgcttt tgaccatttt gggcccgaaa aaaatgaaat cattgcctgt
     3481 gtgaatggct ttcatggccg tactttgttt accgtgagtg tgggtgggca gccaaaatac
     3541 caacaaggct ttgccccatt gccagctggc atcaaacaca ttccatttaa cgatgtggat
     3601 gcattggcag cagccattag cgacaaaacc tgtgcggtgg tgatagagcc ggtgcagggt
     3661 gagagtggtg tgttgccagc aagcaaagac tttttacagg ccgcccgtca attgtgtgat
     3721 gagcacaatg ctttgttggt gttggatgag gtgcaaacag gcatgggccg tacgggcaaa
     3781 ttgtttgcct atgagcattt tggtgtggtg cctgacattg tgtcctcagc taaagccttg
     3841 ggcgcaggct ttcctattgg cgcgatgttg accactgata aagtggcacc aagttttgca
     3901 cctggtaccc atggtagtac gtttggtggt aatcctttgg ccgccgcggt gggaaatgct
     3961 gcctttgaca ttattcatca agcagagact ttggcgcatg tgcgtgccca aggtgagcgc
     4021 ttacaggtcg agttgcgcaa gataggtgaa acacatggcg tgtttgccga agtacgtggt
     4081 ttgggcttgc tcatcggcgc ggttttgacc gatgcctacc atggcaaagc aggtgagttt
     4141 gtaaaagtgg ctttgacgca gggattgatg atattaaatg caggcacaaa tgtggtgcgc
     4201 tttgcgccaa gcttgttatt gagcgatgcc gatttacagg ccgctgtggt taagttgaat
     4261 gccacagccg cacagtttgt ggcggaacaa ggtgtgtaat gtcggccaaa gctaaagtcg
     4321 tggtgttaac aggggctggc atcagtgcag agagcggctt gctcaccttt cgtgatgcag
     4381 gtgggttgtg ggaggggcat aaggttgaag aggtggcaac acccgaagct tttgcgcgca
     4441 atcctcaatt ggttttgaat ttttacaatc agcgcagggc gcaattgcaa caagtcgctc
     4501 ccaatcctgc acatttggct ttggtggatt tggagcaggc atacgatgtg cacatcatta
     4561 cccaaaatgt ggatgatttg catgaacgag cgggcagcag ccacattttg catttgcacg
     4621 gagagctgac caaggtccgc agcagcgtga atgaagatta tgtggttgca tgggatggtg
     4681 atcagcaatt ggatgataaa gacccacaag gttatcccat gcgtccattc attgtgtggt
     4741 tcggtgaagc cgtgccgctg atagagaatg cggtcagcat catcgcgaat gcggatgtgg
     4801 tactggtggt ggggacatcg atgcaggttt atcctgcggc gagtttgttg gattatgcac
     4861 ctttggacat tcctgtgtat tacatagacc ccgctgctca ggccatggag tctgctgtgc
     4921 acattcgtgc agaggtggcc agtgtgggcg tgccagcagt ggtgaaagaa ttattagcag
     4981 cagcaagtta ggatgggtat gtcgtggtta aagccaacca catggttggc tgttctgtgc
     5041 cgccaagtgg cggcatatcg tttttggttg gcactggtgt tggcattgtt tggaacagtg
     5101 ttgttacatg gtttgtggtt gtggttggag gcgttaacag cggctcagga gcatttcttc
     5161 ggacgtggtt tgggtattgt ggccacttgg gtagcgtttg ggttgcaatt tgcttggcct
     5221 gtgttgattt tgacttttgg tgtattgggt gtgtggcagc gctggcgcgg cgacagtgta
     5281 ttcagacgta tgcagcgcca aagcagtcaa gggtttgagc aattgtcttg gcaggatttt
     5341 gaagccttga ttgcagaact tttccgtcgc catggttttg aggttgaaga acaaggtggt
     5401 gatcatgcgg atggcggtat tgatgtcatt gttaagcgtg atgaatgcac atatttggtg
     5461 caatgcaagc attggcaagc gcagcgagtg ggcgtgaaag tggtgcgaga gctgtatggg
     5521 gtgattgctg cagaaggtgt ggatggaggg tatgtgatta cttcaggaca ttttacccca
     5581 gatgcggttg attttgccca aggcattgct atgcaattga tcaatggccg tcaattgatg
     5641 gcattgctgg ccgatgagtt ggagttgtga gtggcaggcc gcatccttca agctcagcaa
     5701 gtttggagtg gtgcggcttg gttgtatgag gtgatgggtt gcggcctgta agtcagatgt
     5761 gcctgctaaa tgactgggtt catgtcattt ggcaggcatt ttttattggg aaagctgctg
     5821 taaccaaggc aataaatggg ccatgactac ggttaaaacg atgatgaaag cacagcctat
     5881 cttggcgatg acgcctgcaa taaagccc
//
LOCUS       DAOMVK010000084         5811 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_3734_length_5811_cov_2.947012, whole genome shotgun sequence.
ACCESSION   DAOMVK010000084 DAOMVK010000000
VERSION     DAOMVK010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 5811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5811
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3734_length_5811_cov_2.947012"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="PK856_04200"
     CDS             <1..501
                     /locus_tag="PK856_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRL01607.1"
                     /translation="SMGGITGLWLAINHPERFLSVTVSNSAAKIGQEGPWRERAALVR
                     EQGLQPIASTAAGRWFTEAFVNSHPDTIREMQHNLAAGSAEGYASCCEALAVADVRAQ
                     LPEIRIPVLVIAGSADPVTTVADGKVMQAAIPACQLHEIAASHISNIEQPDDFNQALQ
                     RFLQNV"
     gene            complement(917..2122)
                     /gene="lhgO"
                     /locus_tag="PK856_04205"
     CDS             complement(917..2122)
                     /gene="lhgO"
                     /locus_tag="PK856_04205"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HRL01608.1"
                     /translation="MSQTHYAIIGGGINGLSVARQILLDHPHAKVVLFEKEARVAAHQ
                     TSHNSGVVHAGLYYEPGSLKATLCRRGVELMRDYCMQNQLPYDQCGKVVVALQPEEEG
                     RLEKIYQRSLANGVQGVRMLSGKDIAKVEPNCIGISALHSPETAIVSYQKIAERIAEE
                     ITFRGGSILLSQQIHAVHQTPNGIQIEYSDGKIHPQRFDHAISCCGLQSDRLVQRSGG
                     DSAPKIIPFFGQYYVLDSAVKNEVKGLIYPVPDPQFPFLGVHFTKRIDGEMTIGPNAF
                     ISFDRESYDAHRLNFKDMFDYLSYPGFWKFALKNAKATVRESKTVLSPQYFLREAAKY
                     VPKLSQAKIYPATRGIRAQAMSKSGKLEDDFVIRKEGNITHIRNAPSPGATSSLAIAE
                     YIVREVMVP"
     gene            2114..2848
                     /locus_tag="PK856_04210"
     CDS             2114..2848
                     /locus_tag="PK856_04210"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRL01609.1"
                     /translation="MTHRNSYLYMKTINQSKTLAQEAFIILRQAILNNELQPDQLYSA
                     TELGVKLGGISRTPVREAAQMLEKAGMVRIEKNRGIRVLSASLTQLIETFQIRLMLEV
                     PLARQAALLRNAEQLAHLQTVFKQFHQAALSENVEHTLAADRDYHLAILAIVSNSKAN
                     QIISECRNTVLLTGKATIPSSRSCLATYQDHIELHQAIEAQNGEAAATAMRNHILNTA
                     QLLVHQESAQREHWHGIDVIKQLQAY"
     gene            3150..4571
                     /locus_tag="PK856_04215"
     CDS             3150..4571
                     /locus_tag="PK856_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRL01610.1"
                     /translation="MSEQNHLKTGLKSRHLSMISIAGVIGATLFVGSGKIIANSGPAT
                     IIAYTLGGILVFLIMRMLGEMAIAQPDTGSFSTYADRAIGRWAGFSIGWMYWFFWSLL
                     LGWESYVAGSVLNSWFPEIPIWVYMLVVTVALVLVNLLNVKNYGEIEFWFALIKVVAI
                     VTFMIIGSLAIVNIWPFGDPTVRGISHLTDQGFMPNGFSPVLTALLGVMFAFMGAEIV
                     TVAASESENPSKEIAKASRTVVWRIILFYVGSIFIAVCLIPYNDPRLQDPTWGTYSVT
                     LADLGVPYIQPIVSFVVLTSVCSCFNSALYTSSRMLYSLSKRGDAHKCMQITGKKTGS
                     PWVGVLVSSLFGFLAVYLTATKKMDVYDVLMQATGTVALLVYFAIAVSQLKMRQKLEK
                     QGVQLQHKMWCFPYLTYVVIIAIPCAIIGMIFEGTYRTEVLYTSLLAVVVVSMGLVAQ
                     KFNIGKSDKTHVQPSKAAGLEAE"
     gene            4915..>5811
                     /locus_tag="PK856_04220"
     CDS             4915..>5811
                     /locus_tag="PK856_04220"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL01611.1"
                     /translation="MKTNNINVNDLVDQAKFTPFHWGVLIWCLLVIIFDGYDLVIYGV
                     ALPLLMKEWGLSPVEAGLLASTALFGMMFGAMSFGTLSDKLGRKKTIMICVAIFSGFT
                     FLGSFAANPIQFGILRFLAGLGIGGVMPNVVALMTEYAPKRIRSTLVAIMFSGYAIGG
                     MASALLGSFLVADYGWKIMFYIAGVPLLVLPLLWKFLPESLMFLTQKGKTEQVAKIVN
                     KIEPSIQASSQTLFVLNEKTAGDEAPLKALFQHKRTFSTLMFWVAFFMCLLMVYALGS
                     WLPKLMLQAGYSLGASMLFLFAL"
BASE COUNT         1407 a         1301 c         1476 g         1627 t
ORIGIN      
        1 tccatgggtg gcatcacggg tttatggttg gccatcaacc atcctgagcg cttcttgtcg
       61 gtgacggtga gcaacagtgc tgccaaaatc ggtcaagaag ggccgtggcg tgagcgggcg
      121 gctttggtgc gcgaacaagg cttgcagccc atagcctcta ccgcagcagg gcgctggttt
      181 actgaagcct ttgtgaacag ccatcccgat accatacgtg agatgcaaca caatctcgct
      241 gcgggcagcg ccgaaggcta tgccagctgt tgtgaagcct tggcggtggc ggatgtgcgc
      301 gcacaattgc ctgaaatacg catacccgtg ttggtgattg cgggcagtgc cgaccctgtg
      361 accacggtgg ccgatggcaa agtcatgcaa gcggcgatac ctgcttgtca attgcatgaa
      421 atcgctgcct cgcacatttc caatattgag cagcccgatg atttcaatca ggcattgcag
      481 cgctttttgc aaaacgtgtg aaatggattc gtgtgtaggt gattgctgat tgtcttgtgt
      541 aaacggcctg taaatccatg atgcagtcgc gaaagcaagt tttaagtctc tctgcgtgtt
      601 tggtaagcta acgcagtttt aagccgctat gcggcttttt ttttgtttta gaaatctaaa
      661 atctgaggtg taaatggttg tgaaaagcga aaatcgatgc gctttatgtt gatttcaact
      721 acagataaat caagggtgaa tgggcatctg ctgataacag gcggctgctt gcatgttcaa
      781 gtcgcatgtt caagtgaggt tgcaagcagc ccgatgtgat gcattgcctt ttataggggt
      841 ttaatgacat gtttcatcca ttaaaccctg atgtttgcaa ctgtttcaat ctgactggtg
      901 caaaccgtca taccaatcaa ggtaccatga cttcgcgcac aatgtattcg gcaatggcca
      961 aagaagatgt ggcgcctggc gagggtgcat tgcggatgtg ggtgatgttg ccttctttgc
     1021 gaatgacaaa atcatcttcc aacttgccgc tcttgctcat cgcttgagcc cgtatgccac
     1081 gggttgcggg gtaaattttg gcttggctga gtttgggcac atatttggcc gcttcgcgca
     1141 gaaaatactg cgggctcaaa acggttttgc tttcgcgcac cgtggctttg gcatttttca
     1201 aggcaaactt ccaaaagcct gggtagctta agtagtcaaa catgtctttg aaattcagtc
     1261 tgtgggcatc atacgattcg cggtcaaaag aaatgaaggc atttgggcct atggtcattt
     1321 cgccatcgat gcgtttggtg aaatgaacgc ccaaaaatgg aaactgaggg tcgggaacag
     1381 ggtaaatcag ccctttgacc tcatttttga ccgcagaatc caacacataa tactgaccaa
     1441 aaaacggaat gatttttggg gcgctgtcgc cgccagagcg ctgtaccaaa cggtctgatt
     1501 gcaggccgca acaactgatg gcatggtcaa acctttgcgg gtggattttc ccatcgctgt
     1561 attcaatttg gatgccattg ggcgtttggt gcacagcgtg gatttgttgc gacaacaaaa
     1621 tgctgccgcc acggaaggtg atttcttcgg cgatgcgttc cgcaattttt tggtaagaca
     1681 caatggcggt ttcgggcgag tgcagggcag agatgccgat gcagttgggc tcaaccttgg
     1741 caatgtcttt gccacttaac atgcgcacgc cttgcactcc gttggccagt gagcgctggt
     1801 agattttttc caaacggcct tcttcttcag gctgcaaagc caccaccact ttgccgcatt
     1861 gatcgtaagg caattggttt tgcatgcaat agtcacgcat cagttcgacg ccgcggcgac
     1921 acaaagtggc ttttaagctg ccaggttcat aatacaggcc ggcatgcacc acgcccgaat
     1981 tgtggctggt ttggtgggcg gctacccgag cttctttttc aaaaagcacc accttagcat
     2041 ggggatggtc tagcaaaatc tgtctggcca ctgacaatcc atttattccg ccgccaatga
     2101 tggcataatg tgtttgactc atcgtaactc ctatctctac atgaaaacca tcaatcaatc
     2161 gaaaactttg gcccaagagg cgtttattat tttgcgtcag gccatactca acaatgagtt
     2221 acagccagac cagttgtatt cggccaccga attgggtgtg aaactcggtg gtatttcacg
     2281 cacgcccgtg cgcgaggcgg cacaaatgtt ggaaaaagca ggcatggtgc gcattgaaaa
     2341 aaaccgtggc attcgggttt tgtctgcttc tttgacccaa ctgatagaaa cctttcaaat
     2401 tcgtttgatg ctggaagtgc ctttggcacg ccaagcggct ttgctgcgca atgctgaaca
     2461 acttgcccat ttgcaaaccg tgttcaaaca gtttcaccaa gccgcgctgt ctgagaatgt
     2521 ggagcacaca ttggcggccg atcgggatta ccatttggcc attttggcca ttgtgagcaa
     2581 cagcaaggcc aatcaaatca tcagtgaatg ccgcaatacg gttttgctta ctggcaaggc
     2641 caccattcct tcttcacgca gttgtcttgc cacttaccaa gaccacatcg aattgcatca
     2701 agcgatagaa gcccaaaacg gcgaagccgc tgccaccgct atgcgcaacc acattttgaa
     2761 taccgctcaa ttgttggtgc atcaagaaag tgcacaacgc gaacattggc atggcattga
     2821 tgtcatcaag caattacagg cttactaaac gttctttgtt tccatcctga aacactcttt
     2881 aaaaattcag tttgcgttca cagtgttttt cattttatta ttgtaacatg ttgcatgcaa
     2941 catgttatga agaatattta acaaaagtgt caagacgaag cagtgcagag ggctctaatg
     3001 ggtctcagcc agcagtattg cttttaaatc tgattaaaaa aatgattctg aaacagcagg
     3061 caaagtccgc aaacgatgtc tatgaagaca gtgtttgcta caaaaaataa aaatgtttga
     3121 ggcgtgattg ccatcacaaa ggaaagagta tgagcgagca aaaccattta aaaacaggat
     3181 tgaaatccag gcacttgagc atgatttcaa ttgcgggggt gataggggcc actttgtttg
     3241 tgggctctgg caaaatcatc gccaattcag gcccagcaac catcattgcc tacaccttgg
     3301 gcggcatctt ggtgtttctc atcatgcgca tgttgggtga aatggccatc gcccaacctg
     3361 acacgggttc gttttcaacc tatgccgaca gagccatcgg acgctgggct ggtttcagca
     3421 taggctggat gtattggttt ttttggtcgt tgctattggg ctgggagtct tatgtcgcgg
     3481 gcagcgtgct gaacagttgg tttcctgaaa ttcctatttg ggtttacatg ttggtggtga
     3541 ccgtggcctt ggttttggtc aatttgctca atgtgaaaaa ctatggcgag attgaatttt
     3601 ggttcgcctt gattaaggtc gtggcgatag tcacgttcat gatcataggc agcttggcca
     3661 tcgtgaatat ttggccattt ggtgatccaa cggtcagagg catctcgcat ttaaccgatc
     3721 aaggtttcat gcccaatggc ttctctcctg tgttaacagc cttgttgggc gtgatgtttg
     3781 cttttatggg cgcagaaatt gtcaccgtgg cggcttctga atctgaaaac cccagtaagg
     3841 aaattgccaa agcctcacgc accgtggtgt ggcgcatcat tttgttttat gtgggctcca
     3901 tctttatcgc tgtgtgtttg attccgtata acgacccgcg cttacaagac ccaacttggg
     3961 gtacatacag tgtgaccttg gccgatttgg gtgtgccgta catacagccc atcgtcagtt
     4021 ttgtggtgtt gacctcagtt tgcagctgct tcaattccgc cttgtacacc agctcgcgca
     4081 tgctgtattc attgtcaaaa cgcggcgatg cgcacaaatg catgcaaatc acaggcaaga
     4141 aaacaggcag tccatgggtg ggtgtgttgg tgtccagtct gttcggtttt ttagcggtgt
     4201 atctcacggc caccaaaaaa atggatgtgt acgatgtctt gatgcaggca acaggaacgg
     4261 tggctttgtt ggtgtatttt gccattgcgg tatcgcaatt gaaaatgcgc caaaaattgg
     4321 aaaagcaagg cgtgcaattg cagcacaaaa tgtggtgttt tccttacctc acctacgtgg
     4381 tcatcatcgc gattccttgc gccatcattg gcatgatttt tgaaggcact taccgcaccg
     4441 aggttttgta cacgtcttta ttggccgtgg tggtggtgag catggggctg gtggcgcaaa
     4501 aattcaatat cggcaaatcc gataaaaccc atgttcagcc tagcaaggcc gcgggtttgg
     4561 aagcggaata attgtgactt aaatatttta atgcagcacc tcactttgac agcggccttt
     4621 acaggccgct ttttttgctt caagtcccgc tcatataaga cacgccttta tggttaacaa
     4681 tgatgcaaac agggaaatct atccattatt tatagattat tcacaatata tgttcatttc
     4741 atcgtttgag cagtgtctga aacttaggcg gtgagcctat tacttaaatt ccaattgaat
     4801 acccgatttg catttgattt tttaggtatg aaatcatgca aattcggttt tggacacacc
     4861 tagaaaattg cctcattgtg tgcaagcaaa gacaccccac aatagaggaa aaacatgaaa
     4921 accaataata tcaatgtcaa cgacttggtt gaccaagcca aattcacccc gtttcattgg
     4981 ggggtactaa tttggtgttt gctggtgatt attttcgatg gctacgattt ggtcatttac
     5041 ggcgtggctt tgcccttgct gatgaaagaa tggggtttga gccctgttga agcaggtttg
     5101 ctcgccagca cggctttgtt tggcatgatg tttggcgcga tgagttttgg cacgctgtct
     5161 gacaaattgg gccgtaaaaa aaccatcatg atatgtgtgg ccatattcag tggcttcacc
     5221 tttttaggct ctttcgctgc caatccgatt caatttggca tcttgcgctt tttggcaggc
     5281 ttgggcatag gtggggtgat gccaaacgtg gtggccttga tgacggaata cgcaccgaag
     5341 cgcatacgca gcactttggt ggccatcatg ttcagtggtt atgccatcgg tggcatggca
     5401 tcggcattgc tcggctcatt cttggtggcc gattacggtt ggaaaatcat gttttacatc
     5461 gcaggcgtgc ctttgttggt gttgcccttg ttgtggaaat ttttgcctga atcgctgatg
     5521 ttcttaaccc aaaaaggcaa aaccgagcaa gtggcgaaga ttgtgaacaa aatcgagccc
     5581 agcatacaag ccagcagcca aaccttattt gtattaaatg aaaaaaccgc gggtgacgaa
     5641 gcgcctttga aagctttgtt ccaacacaaa cgcaccttca gcaccttgat gttttgggtg
     5701 gccttcttca tgtgcttgtt gatggtgtac gccttgggca gttggttgcc aaaattaatg
     5761 ttacaggccg gttattcatt gggcgcgagc atgctgttct tgtttgcctt g
//
LOCUS       DAOMVK010000085         5737 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_3826_length_5737_cov_4.251672, whole genome shotgun sequence.
ACCESSION   DAOMVK010000085 DAOMVK010000000
VERSION     DAOMVK010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 5737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5737
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3826_length_5737_cov_4.251672"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /gene="hemL"
                     /locus_tag="PK856_04225"
     CDS             complement(<1..1065)
                     /gene="hemL"
                     /locus_tag="PK856_04225"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HRL01612.1"
                     /translation="MNRNEALFEQAKQVIPGGVNSPVRAFGSVGGVPRFIKKAQGAYV
                     WDEDDKQYIDYVGSWGPAIVGHAHPEVIEAVREVALDGLSFGAPTEGEVRIAEEIIKL
                     LPSVEQVRLVSSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTIVLAYNDVEQLQNTFSQIGDEIACVILEPIAGNMNMVQPSAE
                     FVQALRGLTEQHGAVLIYDEVMTGFRVALGCAQSLHGIKPDLTTMGKVIGGGMPLAAF
                     GGRKDIMACISPLGGVYQAGTLSGNPLAVTAGLKTLEIIQRPGFYETLNHNCERLLSG
                     LNAAAKTAGVPFSSQYVGGMF"
     gene            complement(1215..1679)
                     /locus_tag="PK856_04230"
     CDS             complement(1215..1679)
                     /locus_tag="PK856_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HRL01613.1"
                     /translation="MAVEIERRFLVKNDVWRGLAEPKLLYQGYLSVEKERTMRVRIVG
                     DEAWVTIKGYISDVSRHEFEYAIPVADATTILEQMCPFKLKKHRTCIAYQGHVFEVDE
                     YFDDNAPLVVAEIELSSEDTLFERPEWLGEEITHDGRFTNAYLSQHPYATWK"
     gene            complement(1688..2104)
                     /locus_tag="PK856_04235"
     CDS             complement(1688..2104)
                     /locus_tag="PK856_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HRL01614.1"
                     /translation="MYLWFKLLHVFFTILWFAGLFAMPRLLLNVSQLTVGTVEYQRLL
                     VMANKLMRYMTPMAVLALLFGLFAVISGQMWGGWAHSKITLGVVLLGYHFYSWRLLQD
                     FHEGRNHYGQRWYRFYSEVPIVLMALALYLVVFKPF"
     gene            complement(2115..2783)
                     /locus_tag="PK856_04240"
     CDS             complement(2115..2783)
                     /locus_tag="PK856_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HRL01615.1"
                     /translation="MNTHKHTFWYYLRRGVYISILAALLLQAWALYQVWYYAQPRTLQ
                     PADAVVVLGAAAWGKNPSPVFKERINFGLSLYQQGLAPKIIFTGGTPVEGYPTEAAVA
                     ARYAGKHGMKQADMLLETQSRDTYENLLNTRALATQNDINSLIVVSDPHHLARAHYIA
                     DDLDMQVQTAPTPTSRYNQSGFGKQAKLWLQESMLLVLYQLGRLFPDTRDVARRQLIP
                     YRNT"
     gene            complement(2770..3138)
                     /locus_tag="PK856_04245"
     CDS             complement(2770..3138)
                     /locus_tag="PK856_04245"
                     /inference="COORDINATES: protein motif:HMM:NF015893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HRL01616.1"
                     /translation="MKYRYYHNEACSKSRAALALLEEVGVTVECVRYLEQIPTVNELL
                     ALTGKLGVPVHELVRSNEAEYAEFSHLDVADDAAWAAAIHAQPRLLQRPILLNDVSAV
                     ICRPPELALTWLAQQGHEHT"
     gene            complement(3147..3482)
                     /locus_tag="PK856_04250"
     CDS             complement(3147..3482)
                     /locus_tag="PK856_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01617.1"
                     /translation="MNWAGKTSMALGLAMGLSSAAWAQTAAESELLSAQKTYQEVLRT
                     TQSQKSTLAQKQGQLNTAKQRLVEVQSSISKLEAEVTAAQSEDAAAQQALQAAGAKLD
                     AAWAAIKSK"
     gene            complement(3497..3928)
                     /locus_tag="PK856_04255"
     CDS             complement(3497..3928)
                     /locus_tag="PK856_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01618.1"
                     /translation="MSYKVLARIATITTLLVLAACQTPNTGSNDGGKWKNIGASANQN
                     VTHEIDTASIKRNGDVVGYRTRMVLKNAGLESTPNLPAFKTGINQYQMYCKNKTYRIT
                     DTELRNATGAVVYQQTFGSQVPLQRITAGSPAQKQYDSVCT"
     gene            4041..>5737
                     /gene="recQ"
                     /locus_tag="PK856_04260"
     CDS             4041..>5737
                     /gene="recQ"
                     /locus_tag="PK856_04260"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HRL01619.1"
                     /translation="MTSATALQLLHDTFGYQAFRGQQAEIVTAVASGHHALVLMPTGG
                     GKSMCYQLPALLREGVAIVVSPLIALMDDQVANLQAAGVAAAAVHSGIEPARLRQIAD
                     DIAHGSLKLIYVAPERLVSERFIRFMHSIHISLFAIDEAHCVSQWGHDFRPEYQQLGF
                     IADEFPDIPRIALTATADEETRADIRHYLRLQDAETFISSFDRPNIYYQVIEKNNGKK
                     QLLQFVKSQGHSVSGIVYCLSRKRVEEVALLLKENGFNAWPYHAGLDMVVREENQKRF
                     NQDDNVIIVATVAFGMGIDKPDVRFVAHLDMPKSIEHFYQESGRAGRDGLPATSWLCY
                     GLNDWVLLRQRIQDGEAEAIQKQIELQKVDAVLSFCETASCRRQLLLAQFDEASEPCG
                     HCDNCLEPPTRFDATVLVQKLLSCVYRVEQRFATSYVIDVLRGKSNDWIARLGHDKLS
                     TFGIGENLSDKEWRSVVRQCVAGGLLSVNLSYNQALQLTDAAKAVLKGEQSLWLRPLK
                     RDKQSGFAAPATAILRTEREERLWQALRTWRKQRATRDNVPAYVVFPDRTLQNLVEAA
                     "
BASE COUNT         1388 a         1564 c         1377 g         1408 t
ORIGIN      
        1 gaacatgcca cccacgtatt ggctggagaa tggcacaccc gccgttttag cagctgcgtt
       61 caggccgctt aataagcgct cacaattgtg gtttaaggtt tcgtaaaatc caggacgttg
      121 gatgatttcc aaggttttca aacctgcggt caccgccaat gggttgcccg ataaagtgcc
      181 cgcttggtac acgccaccca aaggtgaaat gcaagccatg atgtctttgc gcccaccaaa
      241 tgccgccaaa ggcatgccac caccgatgac tttgcccatg gtggtcaaat caggtttgat
      301 gccgtgcaaa gattgggcgc aacccaaagc cacacggaag cctgtcatca cttcatcgta
      361 aatcaacacc gcgccgtgtt gctcagtcaa gccgcgcaag gcttgaacaa actcagccga
      421 tggctgcacc atgttcatat tgcccgcaat cggctccaaa atcacacagg caatttcgtc
      481 accgatttgg ctgaaggtgt tttgcaattg ttccacatcg ttgtaagcca acacgatggt
      541 gtgtttggtg aaatcggcag gcacacctgc tgaacttggg ttgccaaacg tgagcaaacc
      601 actgcccgct ttcaccaaca agctgtcgga atggccgtgg taacagccct cgaatttgat
      661 gatcttatcg cggcctgtaa agccacgcgc caagcggatg gccgacatgg tggcttctgt
      721 gcccgagctg accaagcgca cttgctcgac gctgggcaat aatttgatga tttcttcagc
      781 gatgcgcact tcgccttcgg taggcgcacc aaacgacaaa ccatccaaag ccacttcacg
      841 caccgcttca atgacttcag gatgggcatg gcccacaatc gcagggcccc aagagcctac
      901 gtaatcaatg tattgcttat cgtcttcatc ccacacatac gcgccttggg cttttttgat
      961 aaaacgcggc acgccgccca cgctgccaaa agcgcgcacg ggcgaattca ccccaccagg
     1021 aatgacttgt ttggcttgtt caaataaggc ttcattgcga ttcatagtat tgtccatatt
     1081 ctcggttttt taactcacat gcggcctgta aatgtttaca gtccgcttca tgctgattgt
     1141 aatccatttg gcaccagcct cattgcgtgt gctcaaacac ctcttgtaag ctgcaccaaa
     1201 tctttacagg ccgcttattt ccatgtggcg taagggtgtt ggctcaaata agcattggta
     1261 aagcggccgt cgtgggtgat ttcttcaccc aaccactcag gccgttcaaa cagcgtgtct
     1321 tcgctcgata attcgatttc cgccaccacc aagggcgcgt tgtcatcaaa atactcgtcc
     1381 acctcaaaca catggccttg gtaagcgatg catgtgcggt gtttcttcaa tttgaacgga
     1441 cacatctgtt ccaaaatggt ggtggcgtcg gccacaggaa tcgcgtattc aaactcatgg
     1501 cgcgacacat cggaaatata gcctttgatg gtgacccaag cctcatcgcc cacgatgcgc
     1561 acacgcatgg tgcgctcttt ttccacgctc aaataacctt gatacaacaa tttcggctct
     1621 gccaagccac gccacacatc gtttttaacc aaaaaacggc gttcaatttc cactgccatg
     1681 tgtttcctta aaacggttta aacaccacca aatacagcgc caatgccatc aaaacgatgg
     1741 gcacttcgct gtaaaagcgg taccaacgtt gaccatagtg attgcgacct tcgtgaaagt
     1801 cttgcaacaa gcgccaactg taaaaatggt aacccaacaa caccacgccg agcgtgattt
     1861 ttgaatgcgc ccagccaccc cacatctggc cactgatgac ggcaaacaag ccaaacaaca
     1921 aagccaacac cgccatcggt gtcatgtaac gcatcaattt gttcgccatc accagcaagc
     1981 gctgatactc caccgtgccc accgtcaact gtgacacatt cagcaacaaa cgcggcatgg
     2041 caaacaagcc tgcaaaccac aaaatggtga aaaaaacatg cagcaattta aaccacaaat
     2101 acatgcttcg ccccttaagt gttgcggtat ggaatcaatt ggcggcgcgc cacatcgcgc
     2161 gtatcgggaa acaaacgccc caattggtat aacaccaata acatgctttc ttgcagccac
     2221 aatttggctt gtttaccaaa gccgctttgg ttgtaacgcg aggttggcgt gggcgcggtt
     2281 tgcacttgca tgtccaaatc atcggcgatg taatgtgctc gcgccaaatg atgggggtcg
     2341 ctgaccacaa tcaagctgtt gatgtcgttt tgcgttgcca aagcgcgggt attgagcagg
     2401 ttttcatagg tgtcgcgtga ctgggtttcc agcaacatat cggcttgttt catgccatgt
     2461 ttgcccgcat aacgcgcggc caccgctgct tcagtcgggt aaccctctac gggtgtgccg
     2521 cctgtaaaaa tgatttttgg tgccaagcct tgctggtaca aagacagtcc aaaattgatg
     2581 cgctctttga acaccggtga cggatttttt ccccacgccg ctgctcccaa taccaccacc
     2641 gcatcggcag gctgtagggt gcgaggctgg gcgtaatacc acacttggta caaagcccac
     2701 gcttggagca acagcgcggc gagtatgctg atgtacacgc cgcgtcttag gtaataccaa
     2761 aaagtatgtt tatgtgtgtt catgaccttg ttgagccaac catgtcagcg ccaattcggg
     2821 tggccgacaa atcacagcag acacgtcatt caatagaatc gggcgttgca gcaagcgcgg
     2881 ttgcgcgtgt atcgctgccg cccacgctgc atcgtctgcc acatccaaat gggaaaattc
     2941 agcgtattcg gcttcattgc tgcgcaccaa ctcgtgcaca ggcacaccca attttcctgt
     3001 caatgccaat aattcattca ccgtggggat ttgttccaaa tagcgcacac attccactgt
     3061 cacgcccact tcttcaagca aagccaaagc cgcacgcgac ttagaacagg cctcattgtg
     3121 gtaataacgg tatttcattg ccatccttat ttggatttta ttgcggccca agccgcatcc
     3181 aacttggctc cagcggcctg taaggcttgc tgagcagcgg catcttcaga ttgtgccgca
     3241 gtcacttccg cttccaattt gctgatggaa ctttgaacct caaccaaacg ttgcttggca
     3301 gtgttcaact gtccttgctt ttgagctaag gtggattttt gactttgcgt ggtacgcaac
     3361 acttcttgat aggttttttg tgctgataac aattcagatt ctgccgcggt ctgcgcccaa
     3421 gccgctgaac tcagccccat tgccaaaccc aatgccatgc tcgttttgcc tgcccaattc
     3481 atattctgtc ctcaaattaa gtacacactg aatcatattg cttttgtgca ggtgagcctg
     3541 cggtaatgcg ctgcaatggc acttggctac caaatgtttg ttggtatacc accgcgcctg
     3601 ttgcattgcg taactcggtg tctgtgatgc gataggtttt gtttttgcaa tacatttggt
     3661 attggttgat gcctgtttta aaggcaggca aattcggtgt ggattccaag ccagcgtttt
     3721 tcaacaccat gcgggtacgg taacccacca catcgccatt gcgtttgatg ctggcggtat
     3781 cgatttcatg cgtcacattt tgattggctg acgcgccaat gtttttccac ttaccaccat
     3841 cgttgctgcc tgtgttaggg gtttggcatg cggccaaaac caataaggtg gtgattgtgg
     3901 caatgcgcgc caatacttta tatgacatac aatttcctgt ccattaactg cctgaaagat
     3961 aagcctagtg tagcagcaat tgactgtatt tagcagcaca aagcgtctaa aatagccatt
     4021 ttcctgcctg acctatcgca atgacctctg caactgcctt acaactttta cacgacactt
     4081 tcggctacca agcatttcga ggccagcaag ccgaaatcgt cacggcagtg gcaagcggcc
     4141 accacgcctt ggtattgatg cccacaggtg gcggcaaatc catgtgttac caattgcctg
     4201 ctttgttgcg cgaaggtgtg gccatcgtgg tgtcgccttt aatcgcctta atggacgacc
     4261 aagtagcgaa tttacaggcc gctggtgtgg ccgctgctgc ggtacacagt ggcatagagc
     4321 ctgcgcgctt gcgccaaatt gccgatgaca ttgcccatgg cagcttaaaa ctgatatatg
     4381 tcgcgcctga gcgtttggtc agcgagcgct tcatccgctt catgcattct atccacatca
     4441 gcttgtttgc cattgatgaa gcgcattgtg tcagccaatg gggacacgat ttccgtcccg
     4501 aataccaaca attgggcttt attgccgatg aatttcctga cattccgcgc atcgccttaa
     4561 ccgccaccgc cgatgaggaa acccgtgccg acatccgcca ctatctgcgt ttgcaagatg
     4621 ccgaaacctt catctccagc tttgaccgcc ccaacattta ttaccaagtg atagaaaaaa
     4681 acaacggtaa aaaacaattg ctgcaattcg tcaaaagcca aggccacagc gtcagcggca
     4741 ttgtgtattg cctgtcgcgc aaacgcgtgg aagaagtggc tttgctgtta aaagaaaacg
     4801 gcttcaatgc atggccttac cacgcagggc tggacatggt ggtgcgcgag gaaaaccaaa
     4861 aacgctttaa ccaagacgac aatgtgatta ttgttgccac tgtggcgttt ggcatgggca
     4921 tagacaagcc cgatgtgcgc tttgtcgccc acttggacat gcccaaatcc atcgagcatt
     4981 tttaccaaga atcgggccgc gcaggccgcg atggtttgcc cgccacttcg tggctgtgtt
     5041 atggcctcaa cgattgggta ttgctgcgcc aacgcataca agacggtgaa gccgaagcaa
     5101 tacaaaaaca aatcgaattg caaaaagtcg atgcggtctt atcgttttgt gaaaccgcat
     5161 cgtgccgccg ccaattgctg ttggcgcaat ttgatgaagc ctctgagcca tgcggccatt
     5221 gcgacaattg cctagagccg cccacgcgtt ttgatgccac ggtgttggtg caaaagctgt
     5281 tgtcgtgtgt gtatcgggtg gagcaacgct ttgccacttc gtatgtgatt gatgtgttgc
     5341 gcggcaaaag caacgattgg attgcgcgct tggggcatga caaattgtcc acgtttggca
     5401 tcggtgaaaa cctgtctgat aaagagtggc gctcagtggt gcgccaatgt gtggcaggcg
     5461 gcttgctcag tgtgaatttg tcttacaacc aagccttgca actcaccgat gcggccaaag
     5521 ccgtgctcaa aggcgagcaa tcgctgtggc tcaggccgct caagcgcgat aaacaatcag
     5581 gttttgccgc acctgcaacg gcaatattgc gcaccgaacg tgaagagcgt ttgtggcaag
     5641 ccttgcgcac ctggcgcaaa cagcgcgcca cccgagacaa cgtgccggct tatgtggtgt
     5701 tccccgaccg caccttgcaa aacttggtcg aagccgc
//
LOCUS       DAOMVK010000086         5733 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_3832_length_5733_cov_3.188271, whole genome shotgun sequence.
ACCESSION   DAOMVK010000086 DAOMVK010000000
VERSION     DAOMVK010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 5733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5733
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3832_length_5733_cov_3.188271"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PK856_04265"
     CDS             complement(<1..404)
                     /locus_tag="PK856_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRL01620.1"
                     /translation="MNLFRNIGRQTLNFIQHLGSISLFLLSILRASGTSFARPRLTVR
                     QMYFAGVMSILIIAVSGVFVGMVLGLQGYTQLNKFKAADALGFAVAAGLLRELGPVLA
                     ALLFASSAGGAMTSEIGLMKTTEQLDAMDVMAV"
     gene            complement(401..1207)
                     /locus_tag="PK856_04270"
     CDS             complement(401..1207)
                     /locus_tag="PK856_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL01621.1"
                     /translation="MSTPFIEFDAVSFAYGERPVLHNVSFSIEVGHFAAIMGGSGSGK
                     TTLLRLMTGQLRPDQGRVLINGKDLRQFSRAEQYDFRRKMGVLFQFGALFTDSNVFDN
                     VAFPLREHSTLPESIINDLVTLKLNAVGLRGVEKLMPSELSGGMSRRVALARTIALDP
                     QLMLFDEPFTGLDPISLGVIALLVARITKALQSTSVMVTHDIHQSLNIVDQVIFLADG
                     EVIFSGTPAEIRQTDSPWVHQFVHGEPDGPVQFRYPTATTLEQQLMTAKS"
     gene            complement(1300..2280)
                     /gene="pdxA"
                     /locus_tag="PK856_04275"
     CDS             complement(1300..2280)
                     /gene="pdxA"
                     /locus_tag="PK856_04275"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009118915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HRL01622.1"
                     /translation="MSYPVFAITSGEPAGIGPDICLDLPQFKLPARCVVIADIELMRE
                     RARLLGKPLHIVPYQADSPPSDANTLEVLHQPLAESVQAGILNVNNAPYVIDVIDRAY
                     HGIVAGEFAGMVTAPLHKGIINDAGRPFSGHTEYLADISHTPQVVMLLAGGGLRVALV
                     TTHLPLKDVAAQITPELLSSVIHILHHDLKNKFQIENPRILVTGLNPHAGEQGHLGHE
                     ELDVIIPTLQKLQADGYDVRGPYPADTVFEPFILKNGDAVLAMYHDQGLPVLKYASFG
                     NGVNITLGLPFIRTSVDHGTALDLAGTGQALSGSLQAAIECAVQMLPPQH"
     gene            complement(2340..3266)
                     /locus_tag="PK856_04280"
     CDS             complement(2340..3266)
                     /locus_tag="PK856_04280"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRL01623.1"
                     /translation="MNVKPLAVAALLAMTTSVWAAPAVQSVDRVLAVADKQVITLGEF
                     QDALKRNRAALPKGSSVTDQQLQSQTLSQLVDHSLMVQMASRTGIQVSDTEIKQNMGL
                     VKKTADMTDKQWQTAVRDALLVEKLKQRDLYSKVRITDAEITQAIDNLPEKEKKQSSG
                     HTVTAYLPQHILIAVDSKTKDADALAKIRELRSRISNGGESFESIARSQSQDPGSAKN
                     DGVLGWAVDGMMVPEFENAMKSLPKGALSEPIRSSYGYHLIRVLDTRQQDAGDAELRA
                     KVRKQLLESKSEQLYGNWANQLRQNAYVSVRQ"
     gene            complement(3263..5581)
                     /locus_tag="PK856_04285"
     CDS             complement(3263..5581)
                     /locus_tag="PK856_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HRL01624.1"
                     /translation="MAILKLTFTPIYAALFLGVAHQAYADTAKEPLSLGSKNPHCVAP
                     SATMIVPAKTSGGEALLPDYTRLTADKAAGQTHNRARAEGDVILERNTQVLNAEWVDV
                     DLVNNVTRAGDQFTLSDSANNSNISGEELVYNMDTRQGEAQRSRFETNQEDRRIQGVS
                     EKMAILGDNRYQLQDAKINTCDPGDDSWHIRASQVTTDYNKNVGMARNAALVFKGVPL
                     LYTPWMDFPLNGNRKSGLLLPSIKTGSNGFELEIPYYFNLAPNYDATVAPHLMTDRGL
                     AISGEFRYLQPDYQGRLYGKWLPDDRKDDENNRYELTWQHQQRFTQNLSGGVDFNQVS
                     DDDYVRDFGSRADVQQSNHLNRQAWVDYNNEVWDGEFLARLQVQKYQTLQNNQRTIDV
                     PYQIMPRLSATYLKDFDPYHATLNVFGQFTNFEHHSKQAGQRAVLYPSLGFDFSRPWG
                     YVRPKIGVHATHYNLEDDPTKHDGSISRTLPIFSVDSGLTLDRPYTFGGRDYIQTLEP
                     RLFYTYIPSKDQSRFPNFDTTEDSLTYESLFRENRFSGQDRINAANQVTSALMTRFLE
                     TQTGKERFRAGVGQRFYFKDDDVDLHGNMYKREEGKSDIFVFGGGQIHEKLYGESQWH
                     YNQNLAKTESFSIGAKYEHAPGRNISARYRYDREAEIYSDHFDRSEQIDIGAQWAVNQ
                     NYSLVARQYYSLSEQRPLDQLFGVEYKSDCGCWNASVVAQHYVSDLNQSKNAVFFQLQ
                     LRNLSNLGNNPVEVLRDSIPSYQPLTDEKAKK"
BASE COUNT         1464 a         1377 c         1381 g         1511 t
ORIGIN      
        1 accgccatca catccatggc atccaattgc tcggtggttt tcatcaagcc aatttcactg
       61 gtcatcgcgc caccagcgct gctggcaaac aataaagctg ccaaaacagg gcccaattca
      121 cgcaacaagc ctgctgcaac ggcaaaaccc aaggcatctg cagccttgaa tttgtttaat
      181 tgggtatagc cttgtaagcc caataccatg cccacgaaca cgcccgaaac cgcaataatc
      241 aggatggaca tcaccccagc aaaatacatt tggcgcacgg ttagacgcgg acgggcgaaa
      301 ctggtgcccg aagcacgcaa aatagagagt aaaaataggc taatactgcc caaatgctga
      361 ataaaattca gggtttggcg gccgatgttt cggaatagat tcatgatttc gccgtcatta
      421 attgttgttc aagcgttgtt gcggttgggt agcggaattg gacggggcca tcgggctcgc
      481 catgcacaaa ttggtgtacc caaggtgaat cggtttggcg aatttctgca ggggtaccac
      541 tgaaaatgac ttcgccatcg gccaagaaaa tcacttggtc aacaatgttc aaagattggt
      601 ggatgtcgtg tgtcaccatc acactggtag attgcaaggc tttggtgatg cgggcgacca
      661 acaaggcaat cacgcccaat gaaatcgggt ctaagcctgt aaaaggctcg tcaaacaaca
      721 tcaattgcgg gtcgagagcg atggtacgcg ccaaggccac gcggcgagac atgccgcctg
      781 acaattcaga aggcatcagc ttttccacgc cgcgcaagcc tacagcattg agtttcaatg
      841 tcaccaaatc attgatgatg gactcgggta aggtactgtg ttcacgcagt ggaaaggcca
      901 cattgtcaaa cacattggaa tcggtgaata aggcgccaaa ttggaacaag acccccattt
      961 ttcggcggaa atcgtattgt tcagctcggc tgaattgacg caggtctttg ccattgatga
     1021 gcacccgtcc ttgatcagga cgcaactgac ctgtcatcaa gcgcaatagc gtggttttgc
     1081 cactgccaga accgcccatg atggcggcaa aatggccaac ttcaatactg aagctgacat
     1141 tgtgcagcac tgggcgttcg ccataggcaa agctgacggc atcaaattcg ataaaaggcg
     1201 tgctcatgtg ggtacggtca tctgttaaca gcacatcatg ttaatgaata cccactgata
     1261 aaacaatgct tatggcagta aattgccgtt aactgacatt caatgttgcg gcggtagcat
     1321 ttgtacagca cattctatcg cggcctgtaa acttcccgat aaggcttgac ccgtgcctgc
     1381 caaatccaaa gccgtgccat ggtcaaccga agtgcgtatg aaaggaaggc ctaaagtgat
     1441 gtttacgcca ttgccaaagc tggcgtattt caacacaggc aaaccttggt cgtggtacat
     1501 cgccaatacc gcatcgccat tttttaaaat gaaaggctca aagaccgtat cggctggata
     1561 agggccacgc acatcataac catcggcctg taacttttgt aatgtgggaa taatcacatc
     1621 caactcttca tgccccaaat gcccttgttc gcctgcatgg ggatttaaac ctgtcaccag
     1681 aatacggggg ttttcgattt gaaatttgtt ttttaagtca tgatgcaaaa tgtggatgac
     1741 tgaactgagt aattcaggtg tgatttgcgc ggctacatct ttcaaaggca ggtgtgtggt
     1801 caccaaagcc acgcgtaagc ctccgcctgc cagcagcatc accacttgcg gtgtgtggct
     1861 gatgtcggcc aaatattcgg tatgcccact gaaagggcgg ccagcatcat tgatgatgcc
     1921 tttgtgcaat ggcgccgtca ccatgccggc aaattcaccc gcaacaatgc catggtatgc
     1981 tcgatcaatc acatcaatca cataaggcgc attgtttaca tttaagatac cggcctgtac
     2041 cgattctgcc agaggctgat gcagcacttc taaggtattg gcatcggacg ggggactatc
     2101 tgcctgatag ggcacgatgt gtaagggttt gcctaaaagg cgagcccgtt cacgcatcaa
     2161 ttcaatgtct gcaatcacca cacagcgcgc aggcagttta aattggggta aatccaaaca
     2221 aatgtccggg cctatgcctg caggctctcc actggtgatg gcaaaaacgg ggtaagacat
     2281 ggctactttc aatcaactta aaagataaaa aagctgccag ttggcagctt taagaaaggc
     2341 tattgacgca cggatacgta agcattttgg cgcaattgat ttgcccaatt gccgtacaac
     2401 tgctctgatt tgctttcaag caattgtttg cgcaccttgg cacgcagctc agcatcgccc
     2461 gcatcttgtt ggcgggtatc caaaacacga atcaagtggt aaccatagct agaacggatt
     2521 ggttcgctca aagcaccttt gggcaaggat ttcatggcat tctcaaactc aggcaccatc
     2581 atgccatcca cagcccaacc caatacacca tcgtttttag cactcccagg gtcttgcgat
     2641 tgtgagcgtg cgatggattc aaaagattca ccgccattgc tgatgcgaga gcgtaattca
     2701 cggattttcg ccaaagcatc ggcatctttg gttttggaat ctaccgcaat caaaatgtgc
     2761 tgtggcaaat aagccgtcac ggtgtggcca ctgctttgtt ttttctcttt ttctggcaag
     2821 ttgtctatcg cttgtgtgat ttcagcatcg gtaatgcgta ctttgctgta caagtcgcgc
     2881 tgtttcaatt tttccactaa aagcgcatca cgtaccgctg tttgccattg tttgtcagtc
     2941 atgtcagcgg tttttttgac caaacccatg ttttgtttga tctcggtatc gcttacttgt
     3001 atgcctgtgc ggctcgccat ttgtaccatc aatgagtggt ctaccaattg gctcaaggtt
     3061 tggctttgca attgttggtc ggtgacgcta ctgcctttag gtaaggccgc acgattgcgt
     3121 ttgagtgcat cttgaaactc acctaaggta atcacttgct tgtctgccac tgccaagaca
     3181 cggtcgacac tttgtacagc aggagctgcc caaacgctgg ttgtcatcgc caataaagcg
     3241 gctacagcca atggttttac attcattttt ttgccttttc atcggttaaa ggttggtagc
     3301 ttgggatgct gtcacgcaac acttcaacag ggttattgcc aaggttgctc aaattacgca
     3361 attgtaattg gaagaacacc gcgtttttac tttggtttaa atcactgacg taatgctgtg
     3421 ccaccacact ggcattccaa cacccacaat cactcttgta ttccacccca aacaattggt
     3481 ctaaagggcg ttgttcagac aaagaatagt attggcgggc aaccaaggag tagttttggt
     3541 ttactgccca ctgggcgcct atgtctattt gttcgctgcg atcgaaatgg tcagagtaga
     3601 tttcggcttc cctatcatag cgataacggg cactgatgtt gcggccaggc gcatgttcgt
     3661 atttggcacc tatgctgaag ctttcggttt tagccaagtt ttgattgtag tgccattggc
     3721 tttcaccata cagtttttca tggatttgac caccgccaaa cacgaagatg tctgatttgc
     3781 cttcttcacg cttgtacata ttgccatgta aatccacatc atcgtctttg aaatagaaac
     3841 gctggccaac ccccgctcta aagcgctctt tgcctgtttg ggtttcaaga aaacgggtca
     3901 tcagggcgct ggtcacctga ttagcggcat tgatgcggtc ttggccagaa aatcggtttt
     3961 cacggaacaa agactcatac gttaagctgt cttcggtggt gtcgaaattg ggaaaacggc
     4021 tttggtcttt acttgggata taggtataga acaagcgcgg ttctaaggtt tggatgtagt
     4081 cacgcccacc aaaagtatag gggcgatcta aagtcaaacc tgaatccacg ctgaaaatcg
     4141 gcaaagtgcg gctgatgctg ccatcgtgtt tggttgggtc atcttccaag ttgtaatggg
     4201 tcgcgtgcac accgattttg ggacgtacat agccccaagg gcggctaaaa tcaaacccaa
     4261 gacttgggta gagtaccgca cgctgtcctg cttgtttgct gtgatgctca aaattggtga
     4321 attgaccaaa cacattcaaa gtggcgtgat acggatcaaa atcttttaaa taagtcgcac
     4381 ttaagcgtgg catgatttga tatggcacat caatggtgcg ttggttattt tgcagcgttt
     4441 ggtatttttg tacttgcaaa cgtgccaaaa actcaccatc ccaaacttca ttgttgtagt
     4501 cgacccaagc ctggcgattc aaatggttac tttgttgcac atcagcacgg ctgccaaagt
     4561 cgcgcacata atcgtcatca gacacctgat tgaaatccac gcctcccgac aaattttggg
     4621 taaagcgctg ctggtgttgc caagtcaatt cataacggtt gttttcatcg tctttgcggt
     4681 cgtctggcag ccatttgcca tacaagcggc cttgataatc gggctgtaaa tagcgaaatt
     4741 caccgctgat ggccagaccg cgatccgtca ttaggtgtgg cgcaactgtg gcatcgtaat
     4801 tgggcgcgag attgaaataa taaggtattt ccaattcaaa accattggaa cccgttttaa
     4861 tggatggcaa aagcaggccg cttttgcgat tgccatttaa aggaaaatcc atccatggcg
     4921 tgtacaacag aggcacgcct ttaaacacca aggctgcatt tcgagccatc ccaacatttt
     4981 tattgtaatc cgttgtcact tggctggcgc gaatgtgcca gctgtcatcg cctggatcac
     5041 aggtgttgat tttagcgtcc tgtaactgat agcgattgtc gcccaatatg gccatttttt
     5101 cgctgacacc ttgaatacgg cggtcttctt ggttggtttc aaaacgcgag cgttgtgctt
     5161 cgccttggcg ggtgtccatg ttgtacacca attcttcacc actgatgttg ctgttattgg
     5221 cactgtcgct gagggtaaat tggtcacctg cacgggtgac attgttcacc aagtccacgt
     5281 ctacccattc ggcattcaat acttgagtat tgcgctctaa aatcacatca ccttctgcac
     5341 gggcgcggtt gtgggtttga cctgcggctt tgtcggcagt caaacgggtg tagtcaggca
     5401 acaaggcttc gccaccactg gttttggcgg gtacgatcat cgtggccgaa ggcgcaacac
     5461 aatgcggatt cttcgagcct aaagacaacg gttctttggc agtgtctgca taagcttggt
     5521 gtgccacgcc cagaaaaaga gcagcataaa ttggagtgaa ggtgagcttc aaaatagcca
     5581 taattgcctt aaaaacgacc catctggtaa aaactgttgg cagcttacca ctgagtgggg
     5641 gtggttagcg acgctaaaca tgggaaaact aggttaaaat gccatttatt ttaccttgaa
     5701 aacgccatgc aacgacaaca agccttaaat act
//
LOCUS       DAOMVK010000087         5668 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_3903_length_5668_cov_3.281489, whole genome shotgun sequence.
ACCESSION   DAOMVK010000087 DAOMVK010000000
VERSION     DAOMVK010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3903_length_5668_cov_3.281489"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /gene="metG"
                     /locus_tag="PK856_04290"
     CDS             complement(<1..934)
                     /gene="metG"
                     /locus_tag="PK856_04290"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRL01625.1"
                     /translation="MSTQRKLLVTSALPYANGNIHLGHMVEHIQTDVWVRFQKMRGHE
                     CYYVCADDTHGTPVMLAAQKKGISPEDMIAQVREDHLADFTGFGIGYDNYYTTHSEEN
                     RELSSEIYQALKANGKIVSRTIEQLFDPEKQMFLPDRFVKGECPSCHAQDQYGDNCEV
                     CGATYSPTELIKPFSAISGATPVLKESEHYFFRLGECEDYLKQWTEGSTTLADGRVQS
                     HLQPEALNKMNEWIEGGLQDWDISRDAPYFGFEIPDAPGKYFYVWLDAPIGYMASFKN
                     LAARLNIDFNEFFKADSQTEMYHFIGKDILYFHAL"
     gene            complement(1114..1581)
                     /locus_tag="PK856_04295"
     CDS             complement(1114..1581)
                     /locus_tag="PK856_04295"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRL01626.1"
                     /translation="MTAPLDAYDKILLRALQADATLSQQRLGQMANLSTAAVNRRLKA
                     LQQTGVIEKHSIVVNPKAVGCGLTVMVEVQVADERMDLLDDMQRQFQRCEHVQQCYYV
                     AGGCDFVLIFLVADMEQYVDLSHQLFHTNSNVKSFKTLVAMNRVKTSMDVPIL"
     gene            1706..2926
                     /locus_tag="PK856_04300"
     CDS             1706..2926
                     /locus_tag="PK856_04300"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HRL01627.1"
                     /translation="MLLANPKVQYQAYPNDLKALMNSEQERLNRQAWAAWRGIAATAT
                     PVYDLPDAAKRLGLGRLSIKDESVRSSLGSFKALGAPIALVRFLMREHPEWEMAAVFT
                     GRYRDVLREQTVISATDGNHGRALAAAAQDCGCRCVIVLHAHVSEEREAAIRAYGAEI
                     VRIKGNYDESVLEAARLAQANGWQVISDTSYEGYEEVPRDVMQGYATIAAEIIEAEEV
                     AQQAAYTHVFLQGGVGGLAAGVVAYWWEHAGAARPKFIVVEPEQADCLWQSAVHGQAS
                     VAQGTVDSIMAGLACGATSPLAWRFLDSSVDAFMTVSDDEAITAMRQLATGSEHDIPV
                     LAGESGVAGLAALNQLAANKQQMAALGLDEHARVLFISSEGATAPSVYAEAAGETAEA
                     VLARQQQWLNAQNT"
     gene            3231..3896
                     /gene="ytfE"
                     /locus_tag="PK856_04305"
     CDS             3231..3896
                     /gene="ytfE"
                     /locus_tag="PK856_04305"
                     /inference="COORDINATES: protein motif:HMM:NF008221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HRL01628.1"
                     /translation="MNLREQSLATIALNINGAAALFRQHQLDYCCGGKQTLAEAAAGQ
                     QVDVAVLEQALVGMGAVQAEQDWRNAPLPDLIDFILQRYHDVHREQFPELIALAQKVE
                     RVHAGKDQVPTGLAAQLQALDEELESHMRKEEQILFPMIRQGNGQQAAMPIRVMEKEH
                     DDAGEMVANILRLTGNLLVPEHACTTWKVLYQGVDEMLADLMNHIHLENSVLFPRALA
                     GER"
     gene            complement(4052..4543)
                     /locus_tag="PK856_04310"
     CDS             complement(4052..4543)
                     /locus_tag="PK856_04310"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRL01629.1"
                     /translation="MKKLLLPALAIIVCGLVAFALFANQRPVATPFSLLDLNNQPVSE
                     KQLQGKVTLINFWYPSCPGCVVEMPKLIDTQAKFAGTAYQTIAISLPFNTEAEVRAYV
                     AENKLPFIVAIDKTGQVGEAYKVTLAPNSFLIDKQGKVIKAYLGEPDWAELHQLIDAE
                     LKK"
     gene            complement(4540..4953)
                     /locus_tag="PK856_04315"
     CDS             complement(4540..4953)
                     /locus_tag="PK856_04315"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRL01630.1"
                     /translation="MIHTTEITVRGYHLDLYRHVNNARYLEFLEEARWQFMDDSGLTH
                     EFDGSKLGLAVVNININYRRGAYVNEQLHIRSQFKKIGHRSAVMEQVVCLKDSDTVVA
                     DATITFVLIDIKTGKALTLDGDVATALNRLLEPAA"
     gene            complement(4958..5332)
                     /locus_tag="PK856_04320"
     CDS             complement(4958..5332)
                     /locus_tag="PK856_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HRL01631.1"
                     /translation="MYEVNRSVLVLVPQAPFWNWLTHLPEINLDGLELEDLQADANAY
                     LIDPVDEIDDAWDFVSARIDTVFSAELADWCEDSSLWPDLHPDIFFEWFDVRLSSILT
                     DLTHAPLDREAFEPLDLNDSEQ"
     gene            complement(5388..>5668)
                     /locus_tag="PK856_04325"
     CDS             complement(5388..>5668)
                     /locus_tag="PK856_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01632.1"
                     /translation="DDTNIDDLVPSEADTPAPVELEAPATDATSSLNQQSNSLETDDS
                     AMAPITEGQALYAELTQYVAPAQAEPTLTAQAVELAPAQDANHLALLY"
BASE COUNT         1359 a         1280 c         1617 g         1412 t
ORIGIN      
        1 acaaggcgtg gaaatacaaa atgtctttgc cgatgaaatg gtacatctcg gtttggctgt
       61 cggctttgaa gaattcattg aaatcgatgt tcaaacgcgc ggccaagttt ttgaatgagg
      121 ccatgtaacc aatcggcgca tccaaccaca catagaagta tttacctggg gcatcgggga
      181 tttcaaagcc gaaataaggc gcatcgcggg aaatgtccca gtcttgcaag ccgccttcaa
      241 tccactcgtt cattttgttc aaggcttcag gctgcaaatg gctttgcacg cggccatcgg
      301 ccaaggtggt gctgccttct gtccactgtt tcaaatagtc ttcacattcg cccaagcgga
      361 agaaatagtg ttccgattct ttcaacacag gtgttgcgcc tgaaatggct gagaaaggct
      421 taatcaattc ggtggggctg taagtcgcgc cgcacacttc gcaattgtcg ccgtattggt
      481 cttgcgcgtg gcagcttggg cattcgcctt tgacaaagcg gtcgggcaag aacatttgct
      541 tttcagggtc gaacaattgt tcgatggtgc gtgacacgat tttgccattg gcttttaagg
      601 cttggtaaat ttcagacgac aactcgcggt tttcttctga gtgggtggtg tagtaattgt
      661 cgtagcctat gccaaagcca gtgaaatcgg ccaagtggtc ttcgcgcact tgcgcaatca
      721 tgtcttcggg gctgatgcct tttttctgcg cggccaacat cacaggcgtg ccgtgggtat
      781 cgtcggcgca aacgtaataa cactcatggc cgcgcatttt ttggaaacgc acccacacgt
      841 cggtttggat gtgttcgacc atgtggccta aatggatgtt accgttggca taaggtaggg
      901 cgctggtcac cagcaatttg cgttgtgtgc tcatggtgtt gttttgtcgg caataaaaaa
      961 atgagcttga ttatagcaga ctccactggc cacggttgtg agctggggac tgtgttttga
     1021 tttaaataag gccttggtaa gcggcctgta agggtttaca ggccgcttat ttgataaagc
     1081 gtaagcgcag gcatggtggt gacttgaatg tgtttaaaga atgggcacat ccatgctggt
     1141 tttcacgcgg ttcatcgcta ccagagtctt gaagcttttg acattgctgt tggtatggaa
     1201 caattggtgg cttaagtcca cgtattgctc catgtcggcc accaaaaaaa tcaacacaaa
     1261 atcacagcca cccgccacgt aataacactg ttgtacatgc tcgcagcgct gaaactgacg
     1321 ctgcatgtca tcaagcaagt ccatgcgctc gtctgccact tgcacttcaa ccatgacggt
     1381 gagcccacaa cctacggctt tgggattgac cacgatgctg tgtttttcaa tcacgcctgt
     1441 ttgttgtaag gctttcaaac gccgattgac ggcggcagtg gaaagattgg ccatctgtcc
     1501 caagcgctgt tgggacaggg tggcatcggc ctgtaaggcg cgcagcagga ttttgtcgta
     1561 agcatcgagt ggggcagtca tgaaaaatca ttccattatt gtcggttaaa tcaataaaac
     1621 attgcgtttt acaggctgaa tttcagaaaa tcatgggtgg gtttgcgatt attatttcaa
     1681 acactgaaac gaaaaggaag cctccatgtt gttagccaat ccaaaagtgc aataccaagc
     1741 ctatcccaat gatttgaaag ccttgatgaa cagcgagcaa gaacgcttaa accgccaagc
     1801 ttgggcggcg tggcgcggca ttgccgccac cgccacgcct gtgtatgact tgcccgatgc
     1861 ggccaagcgt ttgggcttgg gcaggctcag tatcaaagac gaatcggtgc gctcaagctt
     1921 gggcagcttc aaagccttgg gggcgccgat tgccttggtg cgttttctca tgcgcgaaca
     1981 tcctgaatgg gaaatggcgg cggtgtttac aggccgctat cgcgatgtat tgcgtgagca
     2041 aaccgtcatc agcgccaccg atggcaatca tggccgcgct ttggcggcag cggcgcaaga
     2101 ttgtggctgt cgctgcgtga ttgtgttgca cgcacacgtg agtgaggagc gtgaggctgc
     2161 catcagagcc tacggtgcgg aaattgttcg catcaaaggc aattacgatg agtcggtgct
     2221 ggaggcggcg cgtttggcac aagcaaacgg ttggcaagtg atttccgaca cttcgtatga
     2281 aggctatgaa gaggtgccgc gcgatgtgat gcaaggttat gccacgattg cggctgaaat
     2341 catagaagca gaagaagtgg cacaacaagc ggcgtacacg catgtgtttt tgcaaggcgg
     2401 cgtcggtggc ttggcagcgg gtgtggtggc gtattggtgg gaacatgctg gtgcggcgcg
     2461 gcccaagttc attgtggtcg agcctgagca agccgattgt ctgtggcaaa gcgctgtaca
     2521 tgggcaggcg agtgtggcgc aaggtacggt ggattccatc atggcgggct tggcttgtgg
     2581 ggcaacttcg ccattggcat ggcggttttt ggacagcagt gtcgatgctt tcatgaccgt
     2641 cagcgatgac gaagccatca cagcgatgcg acagttggcg acaggaagtg agcacgacat
     2701 tcctgtgttg gcgggggagt cgggtgtggc gggcttggcg gccttaaacc aattggcagc
     2761 gaacaaacag caaatggcgg ccttgggttt ggatgagcat gcgcgtgtgc tgttcatcag
     2821 ctctgagggc gcgaccgcgc cgagtgtgta tgccgaggcg gcaggtgaaa cggcagaagc
     2881 ggtgttggcg cgacagcaac agtggttgaa tgcacaaaac acttaagcgc agtcaattga
     2941 agcacaatca attgaggcac actctagcgg cctgtaaacc tttacaggcc gcttgttaat
     3001 ggattcgtca ttaacgatgt tcagcaatca aaccacaagc atggctcaaa tagaaggaac
     3061 cgatgccgtt gggtatcttt aatcatgagc caagcgacgt aagcccactc atcctttgtg
     3121 cgtatgggca agagcatgag cgtggtttac attgaagcca agacagagat gacggtggtc
     3181 agatttgagc accgtttggt ttataggcgc aatgggtatg gagaatgggc atgaatttac
     3241 gtgaacaatc attggcaaca atcgcattga acatcaatgg cgcggcggca ttgtttcgtc
     3301 aacaccaatt ggactattgc tgtggtggca aacaaacctt ggctgaagcc gcggcagggc
     3361 agcaagtgga tgtggctgtg ttggagcagg ctttggtggg catgggcgcg gttcaggctg
     3421 agcaagattg gcgcaacgcc ccattgcctg atttaatcga tttcatcttg cagcgctacc
     3481 atgatgtgca ccgtgagcaa tttcctgagc tgattgcttt ggcgcaaaaa gtggagcgtg
     3541 tgcatgcggg caaagaccaa gttccaactg gcttggcggc gcaattacag gctttggacg
     3601 aggaattgga aagccacatg cgcaaagaag agcaaatact gtttccgatg atacgacaag
     3661 gcaatgggca gcaagccgcc atgcccatcc gtgtgatgga aaaagagcac gatgatgcag
     3721 gggaaatggt ggcaaatatc ttgcgtttaa cggggaattt gcttgtgccc gagcatgctt
     3781 gcaccacatg gaaggtgctc taccaaggtg tggatgaaat gctggccgat ttaatgaacc
     3841 acatccattt ggaaaacagt gtgttgtttc ctcgcgcctt ggcaggggag cgctgaaagg
     3901 tgtgaattgc tggagcatga gtcagggcgg tgatagaagt gtgattaatg atggatggga
     3961 attggtcagc accgcatgaa acgggataat gacgatgtga aagcggcctg taaatgttta
     4021 caggccgctt gagggtttta aatcatgcca atcatttctt caattctgca tcgattaatt
     4081 ggtgtaactc tgcccaatcg ggttcgccca aataggcttt gatgactttg ccttgtttat
     4141 ctatcaagaa agaattgggt gccaaagtga ctttgtaggc ttcaccgact tggcctgttt
     4201 tgtctatggc cacgataaaa ggcaatttgt tttcggccac ataggcgcgc acttcggctt
     4261 cggtattgaa cggcaaggaa atggcgatgg tttgataagc cgtacctgca aacttggctt
     4321 gggtgtctat gagcttgggc atctcaacca cacagcctgg gcaggaggga taccaaaaat
     4381 tgatgagggt taccttgcct tgcagctgtt tttcagacac aggctgattg ttcaaatcaa
     4441 gcaaagagaa tggcgtggcg acagggcgct ggttggcgaa caaggcaaac gccaccaagc
     4501 cacacacgat gatggccagc gcaggcaata agagtttttt catgcagcag gttctaataa
     4561 acggttcaat gcggtggcga cgtcgccgtc taaagtgagc gccttgcctg ttttgatgtc
     4621 tatgagcaca aacgtgatgg tggcatcggc caccaccgtg tcgctgtctt tcaaacacac
     4681 cacttgctcc atcacggcgc tgcggtggcc aatttttttg aattggctgc gtatgtgcaa
     4741 ttgctcattc acatacgcac cgcgacggta attgatgttg atgttgacca cggccaagcc
     4801 gagtttgctg ccatcaaatt catgggttaa acctgagtcg tccataaact gccaacgcgc
     4861 ctcctccaaa aattcgaggt agcgcgcgtt gttgacatgg cggtacaaat ccaaatggta
     4921 gccgcgtaca gtgatttcgg tggtgtgtat catgcgctta ttgttctgag tcgtttaaat
     4981 caagaggctc aaaggcttcg cggtcgagtg gggcatgggt caaatcggtg aggatgctgg
     5041 acaagcgcac atcaaaccac tcgaagaaaa tgtctggatg caaatcaggc cacaggctgc
     5101 tgtcttcaca ccaatcggcc agttcggcac tgaatacggt gtcgatacgg gccgaaacaa
     5161 agtcccaagc atcgtcaatt tcatcgactg ggtcgattaa ataagcattg gcatcggcct
     5221 gtaagtcttc cagttccaag ccatctagat ttatttcagg caagtgggtc aaccaattcc
     5281 aaaaaggggc ttggggtacc aaaaccaaga cgctgcgatt cacctcgtac atggtgttct
     5341 ccttcacaat caattaaatg gccaaactat tgggggaatt caatgcttca gtataacaaa
     5401 gccaagtggt ttgcatcttg cgcaggcgca agttcaacgg cttgggctgt cagtgtgggc
     5461 tctgcctgag caggggctac atattgggtt aattctgcat acagcgcttg gccttctgtt
     5521 attggggcca tggcactgtc atctgtttca aggctgttgc tttgctgatt caggctggac
     5581 gttgcgtctg tagcaggtgc ttccaattcc actggtgcag gcgtgtctgc ttcagagggt
     5641 accaaatcat caatattggt gtcatcgt
//
LOCUS       DAOMVK010000088         5644 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_3937_length_5644_cov_2.306316, whole genome shotgun sequence.
ACCESSION   DAOMVK010000088 DAOMVK010000000
VERSION     DAOMVK010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3937_length_5644_cov_2.306316"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PK856_04330"
     CDS             <1..487
                     /locus_tag="PK856_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRL01633.1"
                     /translation="DEEALYGPLNNERQMAHVSGFISRLPAHAKIETGGKAGERAGFY
                     FEPTVVTGLQQDDEAIQTEIFGPVITVQKFTSDDEALAMANGVDYGLASSVWTRSNAR
                     VMRFSKDMDYGCVWINTHIPLTAEMPHGGFKMSGYGKDLSMYGLEEYTRIKHVMNAHD
                     D"
     gene            579..1064
                     /locus_tag="PK856_04335"
     CDS             579..1064
                     /locus_tag="PK856_04335"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HRL01634.1"
                     /translation="MQALKLTQAGFLALALAASSAAFAKDTPQHIIQNYMAAWNEHNP
                     DKAATYLADDAVFYDVTVGTPQQGKIAARDNVMRVFIGAVPDLKWEMTSKPIVGKDGN
                     IAFQWKFTGRNTGAWGADTPATGKSLSFEGVSFIRMQGDKIVYQGDYYDALNFNKQLG
                     W"
     gene            1288..2013
                     /locus_tag="PK856_04340"
     CDS             1288..2013
                     /locus_tag="PK856_04340"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRL01635.1"
                     /translation="MFTHAVIFAPLGQATRSEQVVDRLKNAIVTGLLEANEQLPNEAE
                     LAKLMGVSHITIREALNTLRNKNLIHTVRGRNGGSFVCELPKEQLRAEHPLREISSAF
                     LADLGELHAAVLSHSAKLACERSTPNDLEKLAEFIEVFAQAKTPETRAQADMRCLLTL
                     AAHAQSARLANLELAIQAEWASLVAMIYTDALHAEVVQLYRDLQAALNAHDSSKAAHI
                     ANEMLQTLTDALIASKFSYDQNQ"
     gene            2083..2793
                     /locus_tag="PK856_04345"
     CDS             2083..2793
                     /locus_tag="PK856_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HRL01636.1"
                     /translation="MHTTEKTEKLLAQLTDIFGSIATATEQLANELAQVFAEHLHIDS
                     EDSLLLTPHIRGVFQTHIQAILKRNPYCTGAGFASHIHVKSKDHDYWMLEWWLKDNND
                     IKQALYELDQGTQQRLDFRTFEWFHKTHQHNATYIHGPYVDYICNTDYTITSAHAVSV
                     NGQFVGVAVIDILVAKLEQLLAPFLGADSPKVLLSNLENRVIVSNSPRIRTGSIYQAS
                     GQTSPVATIAEHFKLYAV"
     gene            complement(2897..4414)
                     /locus_tag="PK856_04350"
     CDS             complement(2897..4414)
                     /locus_tag="PK856_04350"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HRL01637.1"
                     /translation="MNDHSVKEELKHVAEEAKHLAQAVDGKATQFERSIGLISNFSLG
                     FTYLSPLTAVYSLFALAITLAGPPAFWWVVIAACGQLLVAVVFGETASQYPITGGLYP
                     WARRLWGRKYAWMAAWVYLWALVVTVTSVAEYSATFVASLLGYEANAINGLITSVILL
                     ILCLVVNMSGTKNLARVARVGFWCEIVSVIALGIYLLIFHRAQPFSVIFDTMGVVASN
                     GSYITAFMAASLIGLFMFFGFEACGNVAEEVKNPSRQIPVAMILSIVFGAVSALVSIL
                     GYLLASPNLAAIVSGQIADPIPAILNDALGPMGGKIFVLIAIIAMVTCILSLQAALSR
                     LIFSFARDEMLPKSAWMSKLSKNGVPDHAMVISCLLPLLICVWVYFQPDNLARITAFA
                     VIGIYISFQMVVFAALRQRLQGWKPAGEWTVGRWGMPINILALLYGVSAIYLLAQPAE
                     SESFFDQWIVLIGLTVVIGSGLLYMLIAKPYGKSNAPENDAIAHAAAMRAQRQGK"
     gene            complement(5011..5550)
                     /locus_tag="PK856_04355"
     CDS             complement(5011..5550)
                     /locus_tag="PK856_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese family protein"
                     /protein_id="HRL01638.1"
                     /translation="MNTRLVNVSQAQQLLTQGYRLIDIRSADEYRREHIVGAENITPN
                     DLSQHDLHQAQVIFTCLSGMRTQSCAGQLAQATGSEEVLLLDGGLNAWKKAQGEVVKN
                     TAEPLPLMRQVQIVAGSLIVLGVVLGHAVHAGFFLLAGFVGAGLVFAGVTGFCGMAVL
                     LMKMPWNKNSGHTGTSCSL"
BASE COUNT         1579 a         1529 c         1242 g         1294 t
ORIGIN      
        1 tgacgaagaa gctttatatg gcccactcaa caatgagcgc caaatggcac atgtctctgg
       61 cttcatcagc cgcttgcctg ctcacgccaa aatcgaaaca ggcggcaaag caggcgaacg
      121 cgcaggcttt tactttgagc caaccgtggt gacgggtttg caacaagacg acgaagccat
      181 ccaaaccgaa atcttcggcc ccgttattac ggtgcaaaaa ttcaccagcg acgacgaagc
      241 cttggccatg gccaatggcg tggattatgg tctggcttcg agtgtgtgga cgcgcagcaa
      301 tgcccgcgtg atgcgtttta gcaaagacat ggactatggc tgtgtgtgga tcaacaccca
      361 cattcctctc acggccgaaa tgccacacgg tggtttcaaa atgtcgggct atggcaaaga
      421 cttgtccatg tacggcttgg aagaatacac ccgcatcaaa cacgtgatga atgcccacga
      481 cgattgattt aggcatgaca agcggcctgt aaaccctgtt tcaaggctta caggccgctt
      541 attcaggttc actgtctcat tttgataaag gaatcaccat gcaagcattg aaactcactc
      601 aagcaggttt tctcgccttg gccttagccg ccagcagcgc agcgtttgcc aaagacacgc
      661 cgcagcacat cattcaaaac tacatggccg cttggaatga acacaatcca gacaaggccg
      721 ccacttattt ggccgacgac gcagtgtttt acgatgtcac cgttggaaca ccacagcaag
      781 gcaaaatcgc cgcgcgcgac aatgtgatgc gcgtgttcat cggcgctgtg cccgatttga
      841 aatgggaaat gaccagcaag cccatcgtag gcaaagacgg caatattgcc ttccaatgga
      901 aatttacagg ccgcaatacg ggtgcttggg gcgcagacac gcctgccaca ggtaaaagcc
      961 tgagttttga aggggtgagc ttcatccgca tgcaaggcga caaaatcgtt taccaaggcg
     1021 attattatga tgctttgaat ttcaacaagc aattgggttg gtagcctgca cgtgaggcaa
     1081 atcccgcgat tttgcctcac tcaccttgct gtgcacttgt ccacagacat gccatgagac
     1141 tgtgtaggtt tgttttcagc aaatcgattt ggggcagcac aagcaattgc tttataatca
     1201 cggcatattt ggcctcttgc cgcaccgaaa cacccgtctt taggcctgtt cattctcaca
     1261 ttaagcaccg ataaggtctt cgattccatg ttcacacacg ccgttatttt tgcacccttg
     1321 ggccaagcaa cccgttccga acaagtggtg gatcggctca aaaatgccat cgtaacgggc
     1381 ttgctcgaag ccaatgaaca attgccgaat gaggccgaat tggccaaatt gatgggcgtg
     1441 tcgcacatca ccatccgcga agccttgaac accctcagaa acaaaaacct catccacacc
     1501 gtgcgcgggc gcaatggtgg cagttttgtg tgtgaattac cgaaagaaca attgcgggcc
     1561 gaacacccac tgcgcgaaat cagcagtgca tttttggccg acttgggcga gctgcacgcg
     1621 gcagtgttaa gccacagcgc caagctcgct tgcgagcgct ctacccccaa cgatttggaa
     1681 aaactggctg aatttattga ggtgtttgcc caagcgaaaa ccccagaaac ccgtgcccaa
     1741 gccgacatgc gctgcttgct cacgctcgct gcccatgccc aatcggcacg tttggcgaat
     1801 ttggaattgg ccatccaagc cgaatgggca tctttggtgg ccatgatata caccgatgcc
     1861 ttgcacgctg aagtggtaca gctttaccgt gatttacagg ccgctttgaa cgcgcatgac
     1921 agcagcaaag ccgcccatat tgccaatgaa atgctgcaaa ctttgacaga tgccttgatt
     1981 gcctctaaat tttcttacga tcaaaaccaa taacatcacc aatgcgccac cccgtggcca
     2041 acatcgcaaa cccatgccgt aaacccacag gtaatgactt gtatgcacac tacagagaaa
     2101 acagagaaac tgctggcgca attaaccgac atcttcggct ccatcgcgac agccaccgaa
     2161 caattggcca atgaactggc gcaagtgttt gccgagcatt tgcacatcga cagcgaagac
     2221 agcctgctgc tcacccccca catccgtggg gtatttcaaa cccacattca agcgatttta
     2281 aaacgcaatc cgtattgcac gggcgcaggt tttgccagcc acatccatgt caaaagcaaa
     2341 gaccacgatt actggatgct cgaatggtgg ctcaaagaca ataacgacat caaacaagcc
     2401 ttgtatgagc ttgaccaagg cacgcaacaa cgcctggatt tccgtacttt tgaatggttt
     2461 cacaagaccc accaacacaa tgccacctac attcatggcc cgtatgtgga ctatatttgc
     2521 aacaccgatt acaccatcac ctcggcacat gccgtcagcg tgaacgggca gtttgtcggc
     2581 gtggcggtca ttgatatctt ggtggccaag ttggagcaat tgctggcgcc ttttttggga
     2641 gcggattccc cgaaagtatt gctctccaat ttagaaaacc gtgtcatcgt ctccaatagc
     2701 ccacgcattc gcactggttc tatttaccaa gcaagtggac aaacctcacc tgttgccacc
     2761 atcgctgagc atttcaaatt gtatgccgtt taaacatcgc tgcatttttt aacaccatca
     2821 cgtttgctca caaaaaaggc ttaatcctaa gattaagcct tttcacatcc atacttaatg
     2881 acacctactt ctgaacttac ttgccttggc gctgcgcccg catggcagcg gcgtgagcga
     2941 tggcatcgtt ttcaggcgca ttagacttgc cataaggctt ggcaatcagc atgtacaaca
     3001 agccactgcc tataaccaca gtcaaaccga ttaacacgat ccactggtca aaaaacgatt
     3061 cgctttccgc aggttgggcc aacaggtaaa ttgctgacac accatataac aaagccaaga
     3121 tgttgatagg catgccccaa cggcccaccg tccactcacc cgcaggtttc cagccttgta
     3181 agcgttgacg caaagcggca aacaccacca tttggaaaga gatgtagata ccaatcaccg
     3241 caaacgccgt gatgcgcgcc aaattgtcgg gttggaaata cacccacacg caaatcaaca
     3301 atggcaacaa gcagctgatg accatggcgt gatcgggtac accatttttc gacaatttcg
     3361 acatccatgc actttttggc aacatttcat cgcgagcaaa cgagaagatc aaacggctca
     3421 aagcggcctg taacgacaaa atacacgtca ccatggcaat gatggcaatc aacacgaaga
     3481 ttttgccgcc catagggccc aacgcatcgt ttaagatggc gggaattggg tcggcgattt
     3541 gacctgacac aatcgcagcc aagttaggcg atgccaacaa ataacccaaa atagacacca
     3601 aagccgacac cgcaccaaac acgatgctca aaatcattgc cacaggaatt tgacggcttg
     3661 gattcttcac ttcttcggcc acgttgccac aggcttcaaa accaaagaac atgaacaaac
     3721 cgattaatga tgccgccata aacgcggtga tgtatgagcc gttgctcgcc accacaccca
     3781 tggtgtcaaa aatcacagag aacggttggg cacggtggaa aatcaacagg taaataccca
     3841 aggcaatcac gctgacaatt tcgcaccaaa agcctactcg cgccacgcgc gccaagtttt
     3901 tcgtgcccga catgtttacc accaaacaca aaatcagcaa gatgaccgag gtaatcaaac
     3961 cattgatggc gttggcttcg tagcccaaca aagacgccac aaatgtggcc gaatattcgg
     4021 caaccgacgt cacggtcacc accaaggccc acaaatacac ccacgccgcc atccatgcgt
     4081 atttgcgccc ccacaaacgg cgcgcccaag gatacaggcc gcctgtaatc gggtattgcg
     4141 aagcggtttc accaaacacc accgccacca acaactgacc acatgcggca atcaccaccc
     4201 accaaaacgc aggtggccct gccaaggtaa tggccaaggc aaacaaggaa tacaccgccg
     4261 tcagcggtga caaataggta aaacccaagg aaaaattgga aatcaaaccg atggaacgct
     4321 cgaattgcgt ggctttacca tcgactgctt gggccaaatg cttggcctcc tctgcgacat
     4381 gctttaactc ttctttgaca gaatgatcat tcatattcaa ctcctgctgc ttaatgtgtg
     4441 ggtatcttga gagatgaaac gtgaaaccaa taccagctta catcgccgcc gttccaagac
     4501 tgagcctgta tcaaactgca tggattctcc tttgtgtagc aatgcttctt atctaaatgt
     4561 aagaatgtgt aatttataaa acatattaca ttatatgtat attgtttttt gtagccttgg
     4621 tcgtaaatta agaataacaa attgaattta aactatattt attaaaatag aattttgtat
     4681 agatacggaa tgttttaaga attggatatg taatacagct tagaatcgga ctttgggtat
     4741 aaaccgcaca cacaaaagcg gcctgtaaat gatttacagg ccgctttcaa aatgccactc
     4801 aacacatggc atcacatcaa actgaaatct gtttttgatg ccaaactgcc agtccaactt
     4861 tgcactcaag cccaccattg ccaatgccgt cacaagcagt gcccttcgcc ttcagacact
     4921 ttaactcttg ctagcagcgt gtgcattgac agtggtaata atctacccat tttcttggat
     4981 tctttgtgtg ctcaatacag atttatctgt ttacaaactg caagacgtgc ctgtgtggcc
     5041 gctgtttttg ttccatggca ttttcatcaa caacaccgcc atgccgcaaa aaccagtcac
     5101 gcccgcaaac accaagcctg cgcccacaaa acctgccaac aagaaaaagc cagcatgcac
     5161 ggcatggccc aaaaccacac ctagcacaat caacgagcct gccacaattt gtacttggcg
     5221 catcaaaggc aaaggctctg ctgtgttttt caccacctca ccttgggctt ttttccaagc
     5281 atttaggccg ccatcgagca acaacacttc ttcgctgccc gtggcttggg ccaattgacc
     5341 agcgcagctt tgggtgcgca tgcctgacaa acaggtgaag atcacttgtg cttggtgcaa
     5401 atcgtgttgc gacaaatcat tgggcgtgat gttttcagcg cctacaatgt gttcgcggcg
     5461 gtattcatct gcagaacgga tgtcgattaa gcggtagcct tgtgtcaaca attgttgggc
     5521 ttgactgaca ttcaccagac gggtattcat aaacatcctt ttcattgcct gccacagaag
     5581 caggcgccat ttaatttaga tgataatata ttagatattt ctaaaatatt agtcaacgcg
     5641 gttt
//
LOCUS       DAOMVK010000089         5635 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_3949_length_5635_cov_3.896953, whole genome shotgun sequence.
ACCESSION   DAOMVK010000089 DAOMVK010000000
VERSION     DAOMVK010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 5635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5635
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3949_length_5635_cov_3.896953"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..1582
                     /gene="lysS"
                     /locus_tag="PK856_04360"
     CDS             62..1582
                     /gene="lysS"
                     /locus_tag="PK856_04360"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRL01639.1"
                     /translation="MSEQANQSVETEHLDENQIMALRREKLHTLQQAGNAYPNDFRRD
                     AFAGDLQDAYGEFDKESLAEKKAQVKVVGRMMLKRNMGKASFATIQDVSGQIQLYINN
                     QGVGEDTHEAFKHWDLGDIIACEGEVMKTNHGELTVRASSLRLLTKSLRPLPDKHKGL
                     ADQEAKYRQRYVDLMTNAESRDTFIKRSQIVQTVRNFMAAERYLEVETPMMHTIPGGA
                     TAKPFVTHHNTLDMDLYLRVAPELYLKRLVIGGLERVYEINRNFRNEGMSTRHNPEFT
                     MMEFYEAYADYHRMMDMTENVIRAASVAVNGTSVVTYGDKEVDLEQPFARMTIVEAIK
                     AHNPHYADEQLQDEAWLKTEIKKNGGKLPMAGGLGSLQLALFEEVAESKLWQPTFIVD
                     YPVEVSPLARKSDTNPEITERFELFVVGRELANGYSELNDSEDQARRFQAQVAQLDAG
                     DDEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTNAQTIRDVILFPQMRREII"
     gene            complement(1661..2026)
                     /locus_tag="PK856_04365"
     CDS             complement(1661..2026)
                     /locus_tag="PK856_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01640.1"
                     /translation="MKLWLLPLLAVFAVAACSQETQNKAAETASSAKSDVKEAASAVK
                     EGANNAVEDANKAAETAGEKIDAGTDKAVDATKEATQEVKDGTKELANDATAAAREAG
                     QKAKEGVNDAATAVKEKTE"
     gene            2261..3280
                     /locus_tag="PK856_04370"
     CDS             2261..3280
                     /locus_tag="PK856_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01641.1"
                     /translation="MLNMNEWMKLWGLDPKTGLPTLWKLWWPWLDAEQPMFRMQVRIG
                     GFPIQQPVASSRNVLKQAEVIEQPALEAKQAPKTAETVKNTPAKTVAKKTAVKKDAAP
                     KVAAKTAASKEVTPVAPAVKTTRAKAAAKPVAQTDAVKTTVAAKAAVEKTEVAKTAVR
                     KTATTPAATVKKETAAAPKKTIAAVAKNATTAVKTAEPTATVSKPVAPAKAVAAEKIV
                     AAKPVVTQAEAVKPVATPVAASKETTTSAVKAVATASPVISAKTQQQIDQLGTAPNKE
                     ALRAAIRAVYAEKGAATAEQWGKWLHQNADVLNTLHLQPMAAAGEIKLEQAPAASVVA
                     SKTAE"
     gene            complement(3431..3670)
                     /locus_tag="PK856_04375"
     CDS             complement(3431..3670)
                     /locus_tag="PK856_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HRL01642.1"
                     /translation="MNSLYSYAAQYPHTCNKALYIAMALLFGSFGIHKFCAGRFWMGL
                     LYLVFSWTFIPGVIGIIEGILAVFKTTDDLGAIVV"
     gene            complement(3747..4358)
                     /locus_tag="PK856_04380"
     CDS             complement(3747..4358)
                     /locus_tag="PK856_04380"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HRL01643.1"
                     /translation="MLEKAALRRQLRRQRMAISPSKRRLAQAQVVRLLKPLMRRGKNI
                     GLYLASGSELNISAILQDAATHGCHLHTPYIEPNQRRLWFTPYPAPSHHGHHMLNILQ
                     YEGDKRRIERLDIVLMPLIGIDKRGMRLGQGGGFYDTSLSFCREQQPLRIGIGFAAQQ
                     CEYIPNEAHDQALDAYVCETGITAFSARARATLAPFLNKSHTG"
     gene            4405..4614
                     /locus_tag="PK856_04385"
     CDS             4405..4614
                     /locus_tag="PK856_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01644.1"
                     /translation="MHIIWLGYLFVIMMFCFAQDSMSRGAVLFVLLAVLPTLFVAWML
                     RVRRRNRLSSWEAKMAKENSDVVAK"
     gene            4749..5480
                     /gene="rpsB"
                     /locus_tag="PK856_04390"
     CDS             4749..5480
                     /gene="rpsB"
                     /locus_tag="PK856_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRL01645.1"
                     /translation="MSQVTMRQMLEAGVHFGHQTRYWNPKMAEYIFGARNKIHIVNLE
                     KTLPLFTEAQDFVRRLAANKGTVLFVGTKRQAREIVQEEAIRAGAPYVDYRWLGGMLT
                     NYKTVKQSIKRLEEKSVILENAEQSGLNKKELLDLQREVDKLQRSLGGIKDMKGLPDA
                     IFVIDTGYQKGTIVEAEKLGIPVIAVVDTNNSPEGVKYVVPGNDDSAKAIRLYCRGIA
                     DAVLEGKAQSLQEVQAAVADAKAAE"
BASE COUNT         1532 a         1234 c         1481 g         1388 t
ORIGIN      
        1 cgttaattca gtaggcggcc tgtaaaacat ttacaggctg catttttcat taggaagtgg
       61 catgagcgaa caagcaaatc agtcggttga aaccgaacat ttagacgaaa accaaatcat
      121 ggctttgcgc cgtgagaagt tgcacacttt acaacaagcg ggcaatgctt atccaaacga
      181 tttccgtcgt gacgcctttg caggcgattt acaagacgct tacggtgagt ttgataagga
      241 aagcttggct gagaagaagg cgcaagtgaa ggtggttggt cgaatgatgc tcaagcgcaa
      301 catgggtaaa gccagttttg ccaccatcca agacgtcagc ggtcagattc agctttacat
      361 caacaaccaa ggcgtgggcg aagacacaca cgaagcgttc aaacactggg atttgggcga
      421 catcatcgct tgcgaaggcg aagtgatgaa aaccaaccac ggcgagttga ccgttcgcgc
      481 cagcagcttg cgtttgttga ccaaatcttt gcgtccgttg cctgacaaac acaaaggctt
      541 ggccgaccaa gaggcgaaat accgccaacg ctatgtggat ttgatgacca acgctgaatc
      601 gcgtgatacc ttcatcaagc gcagccaaat tgtgcaaacc gtgcgcaatt tcatggccgc
      661 tgagcgctat ttggaagtgg aaacaccgat gatgcacacc atacctggtg gcgcgaccgc
      721 gaaaccgttt gtgacccatc acaacacttt ggacatggat ttgtatttgc gcgtggcgcc
      781 tgagttgtat ttgaaacgct tggtgatagg cggcttggag cgtgtgtacg aaatcaaccg
      841 caatttccgt aacgaaggca tgtctacgcg ccacaatcca gaattcacca tgatggaatt
      901 ctacgaagcc tatgccgatt accatcgcat gatggacatg actgaaaacg tgattcgcgc
      961 cgcttctgtg gctgtgaacg gcaccagtgt ggtgacgtat ggcgacaaag aagtggattt
     1021 ggagcagccg tttgcacgca tgaccatagt cgaagccatc aaagcgcaca acccacatta
     1081 cgccgatgag caattgcaag acgaagcttg gctcaaaacc gaaatcaaaa agaacggtgg
     1141 caaattgcct atggcaggcg gtttgggcag cttgcaactg gccttgtttg aagaagtggc
     1201 cgaaagcaaa ttgtggcagc caaccttcat tgttgactac ccagtagaag tgtcaccttt
     1261 ggcgcgcaaa tcagacacca atccagaaat caccgagcgt tttgaattgt ttgtggtcgg
     1321 ccgcgagttg gccaatggtt actcagagtt gaacgattcg gaagaccaag cacgtcgctt
     1381 ccaagcgcaa gtggcgcaat tggatgcagg cgatgatgaa gccatgcatt acgatgccga
     1441 ttacatccgc gcgatggaat ttggtttgcc accaacaggt ggttgcggca ttggtatcga
     1501 ccgtttggtg atgttgctca ccaatgcaca aaccatacgc gatgtgattt tgtttccaca
     1561 aatgcgtcgc gaaatcattt aagcggcctg taagccataa gaaaaagccc tgcaagttgc
     1621 cttgcagggc tttttccgac tagaaagggt tggaaaaacc ttattcggtt ttttctttca
     1681 ctgcagttgc tgcgtcattc acgccttctt tggctttttg accggcttcg cgagcggcag
     1741 cagtggcatc gttagccaat tctttggtgc cgtctttcac ttcttgggtg gcttctttgg
     1801 tagcgtctac ggctttgtcc gtaccggcgt caattttttc accagcggtt tcagcagctt
     1861 tattggcatc ttcaacagcg ttgttagcgc cttctttaac agcagaagcg gcttctttaa
     1921 cgtctgattt agcgctgctg gcagtttcag cggctttgtt ttgggtttct tgtgagcaag
     1981 ctgcaacagc aaacacggcc aacaaaggca ataaccataa tttcattgtg aaactccttc
     2041 aaataatgac acagattttg ttgcaacttt gtgcagtgca cagaaggctg catttgacgt
     2101 gttcaggtta gcgatgcgtg acatttttgt acagtgaaaa caatgtgaag agtgtaaatg
     2161 ttgcattgca gcaatgactt ggatattatg gttgccagta aaattaaatc cttgaattgt
     2221 ttgataactg taaacagatt atgtgttttg gaggtggaaa atgttgaata tgaatgagtg
     2281 gatgaagtta tggggtttgg atcctaaaac aggtttgccg acgttgtgga agctgtggtg
     2341 gccatggttg gatgctgagc aacccatgtt tcgcatgcaa gtacgcatag gtggttttcc
     2401 cattcagcaa cctgtagcaa gcagtcgtaa cgtgttaaaa caggctgaag tgatagagca
     2461 acctgcactt gaagcaaagc aagcgcctaa aacagcagaa actgttaaaa acacgcctgc
     2521 caaaacagtg gctaagaaaa ccgctgtgaa aaaagacgct gcgcccaaag ttgctgccaa
     2581 aactgcggca agtaaggaag tgactccagt tgcccctgct gtgaaaacca cacgtgccaa
     2641 agcggcagca aaacctgtgg ctcaaaccga tgcggtgaaa acaactgtgg cggccaaagc
     2701 agcggttgaa aaaacagagg ttgctaaaac cgctgttcgt aaaaccgcaa caacacctgc
     2761 agcaactgta aagaaagaaa ccgcagctgc cccgaaaaaa accattgcgg ctgtggcgaa
     2821 aaatgccaca acggcagtga aaacagccga gccaactgcc acagtgagta agcctgtagc
     2881 tccagcgaaa gccgtagcag cagagaaaat cgttgcggcc aagccagttg ttacccaggc
     2941 ggaagccgtg aaaccagtag cgacacctgt tgccgcgtct aaagaaacaa cgacgtctgc
     3001 tgttaaagct gtggcaactg ccagtcctgt tatttctgcc aaaacccaac agcaaatcga
     3061 tcaactgggc actgcgccga ataaagaagc tttgcgtgcg gcaatccgcg cggtatatgc
     3121 cgaaaaagga gcagccacgg ctgagcaatg gggtaaatgg ttgcatcaaa acgctgatgt
     3181 gttgaatacc ttgcatttgc agcctatggc tgctgcaggc gaaatcaaat tggagcaagc
     3241 tccagcagca agcgttgttg cgagcaaaac agctgaatag tgttggagat cgtttgcatt
     3301 gatgtttaat acagtgcatg cgttatgatt gaaaagcggc ctgtaaatgt ttacaggccg
     3361 ctttcatgtg ctcgtttgct ctgtttgaac gcaaacagaa atgcaggcgt gaagcggggt
     3421 gtttaggggt ttacaccacg atggcaccca agtcatcggt ggttttgaag actgccaaaa
     3481 tgccttcgat gataccaatc acgcctggaa taaacgtcca gctgaaaacc aaatacaaca
     3541 agcccatcca aaaacgccct gcgcagaatt tgtgtatgcc aaaagagcca aacaataagg
     3601 ccatggcgat gtacaaagcc ttgttgcatg tgtgggggta ttgggctgcg taagaataaa
     3661 gcgagttcaa gagtatccct taggttggct gtggtatgac tgataaatgg tgttacaggc
     3721 cgctatttca agcaaattaa acgaatttaa cctgtatgcg acttgttaag gaagggtgcc
     3781 agtgtagccc gagcgcgggc gctgaatgca gtaatgcctg tctcgcacac gtaggcgtct
     3841 aatgcttggt cgtgcgcttc attgggaatg tactcacatt gctgagccgc aaagcctatg
     3901 cctatgcgca acggttgttg ttcgcgacaa aaagacaggc tggtatcgta aaaaccgccg
     3961 ccttggccca agcgcatgcc gcgtttgtct atgccgatta aaggcatgag tacgatgtcg
     4021 aggcgttcaa tacggcgttt gtcgccttca tactgcaaaa tattgagcat gtggtgaccg
     4081 tgatgactgg gtgcgggata aggtgtaaac cacaagcggc gttgatttgg ctcaatgtat
     4141 ggtgtgtgta agtgacagcc atgagtggct gcgtcctgta aaatggcgct aatgttcagc
     4201 tcacttccgc tggccaaata caggccgatg tttttaccgc gccgcatcag tggcttgagc
     4261 aggcgcacca cttgcgcctg agcaaggcgg cgtttggagg gcgagattgc catacgttgg
     4321 cggcgcaatt ggcgacgcaa tgcagctttt tctagcatga ccgtccttgt ctggtggttg
     4381 ggtttgatag gtgaaaggaa atctgtgcac attatatggt tgggttattt atttgtaatc
     4441 atgatgtttt gctttgccca agacagcatg tcacgtgggg cggtgttgtt tgtgttgctg
     4501 gcggtgttgc ccacattgtt tgtggcgtgg atgctcaggg tgaggcggcg caatcgattg
     4561 agcagttggg aagcgaaaat ggcaaaagaa aacagcgatg tggttgccaa gtgattgaaa
     4621 atattttaca atagcggccg cttttcagta gaaaagcatt aactatttaa tgctcacgcc
     4681 gtgcgtcaaa gggtgccttc gggttctgtc gctgcggtgt taggcttaac cctttgagga
     4741 ataagattat gtcacaagtg actatgcgtc aaatgttgga agctggtgtg cactttggtc
     4801 accaaacccg ttactggaac cctaaaatgg ctgagtacat ttttggtgcg cgtaacaaaa
     4861 ttcacatcgt gaacttggaa aaaactttgc ctttgttcac tgaagctcaa gactttgtac
     4921 gccgtttggc cgctaacaaa ggcactgtgt tgtttgttgg taccaaacgt caagctcgtg
     4981 aaatcgtaca agaagaagcc atccgcgcag gtgctcctta tgtagattac cgttggttgg
     5041 gtggtatgtt gaccaactac aaaaccgtta aacaatctat caaacgcttg gaagaaaaaa
     5101 gcgtgatttt ggaaaacgct gaacaaagcg gcttgaacaa aaaagaattg ttagatttgc
     5161 aacgtgaagt agacaaattg caacgctctt tgggcggtat caaagacatg aaaggtttgc
     5221 cagacgcaat cttcgttatc gatactggct accaaaaagg caccatcgtt gaagctgaaa
     5281 aattgggcat ccctgtgatt gcagtggttg ataccaataa cagcccagaa ggcgttaaat
     5341 acgttgttcc tggtaacgat gactctgcta aagcaatccg tttgtactgc cgtggtatcg
     5401 ctgatgcggt attggaaggc aaagcacaat cattgcaaga agttcaagct gccgtggctg
     5461 atgctaaagc tgctgaataa tagcggtttg caaaatatct gaaaaagggg cggctctcgc
     5521 cccttttttg aaattcaggt cggccgattt gttttaagcg gcctgaacaa aacgaacact
     5581 tatttaagga gtaaacaatg gctacaatta ctgcaaaaat ggttggcgat ttgcg
//
LOCUS       DAOMVK010000090         5616 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_3965_length_5616_cov_3.657975, whole genome shotgun sequence.
ACCESSION   DAOMVK010000090 DAOMVK010000000
VERSION     DAOMVK010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3965_length_5616_cov_3.657975"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1288)
                     /gene="mnmG"
                     /locus_tag="PK856_04395"
     CDS             complement(<1..1288)
                     /gene="mnmG"
                     /locus_tag="PK856_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HRL01646.1"
                     /translation="MIYPTLYDVIVVGGGHAGTEAALAAARMGAKTLLLSHNIETLGQ
                     MSCNPSIGGIGKGHLVRELDALGGAMALAADMGGIQFRTLNASKGPAVRATRAQADRV
                     LYKAAIRHMLENQENLDLFQQQVDDVILDGERISGVLTTMGLQFNARAVVLTAGTFLS
                     GKIHIGLENYQGGRAGDPAAMSLGEKLRELQLPQGRLKTGTPPRIDGRSIDFSALSEQ
                     PGDTPTPVFSVRGSRDLHPRQVSCWITHTNSTTHDIIRSGFDRSPMFTGKIEGVGPRY
                     CPSIEDKINRFADKESHQIFLEPEGLDTHEYYPNGISTSLPFDIQIALVRSMKGLENA
                     HILRPGYAIEYDYFDPRNLKASLETKTIQGLFFAGQINGTTGYEEAAAQGLLAGANAA
                     QYVFEREALVLHREQAYLGVLVDDLITKGVNEPYRMF"
     gene            1487..2017
                     /locus_tag="PK856_04400"
     CDS             1487..2017
                     /locus_tag="PK856_04400"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRL01647.1"
                     /translation="MNHKQSSRLDFPHIPLVRYTDDFLQAAAMTPTTRLLQSDDFAAW
                     RPLWQQYLDFYQTELDETVTLHTWQRLIDPKQTDMLGLGVFVDGRLSGFTHLVFHPNT
                     WSAEPCCYLEDLCVDAQVRGQGLGRALIQAAQDLAQQKQCCRLYWVTARDNHTAQLLY
                     NTLAEQTDFIQYKIGL"
     gene            complement(2076..3008)
                     /gene="pip"
                     /locus_tag="PK856_04405"
     CDS             complement(2076..3008)
                     /gene="pip"
                     /locus_tag="PK856_04405"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009116888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HRL01648.1"
                     /translation="MYTVDSVNQSGLLQVSAIHQMYWEDCGNPNGKPVIFLHGGPGGG
                     SSPATRGFFNPEKYRIIQIDQRGCGKSLPHACIEDNDTWKLVADIEVLREHLGIDKWM
                     VFGGSWGSTLSLAYAQTHPERVTELVLRGIFLFRQFESDWLFKRGGASQIFPEAWETF
                     ENFIPEAQRGDLIAAYNHLLFGKDVDATTKIQAARHWCEYEEKIVFLHPQIPQDNDPH
                     ALAISSMENHYFINQGWLQGERALLSNIGKIRHIPTVIVQGRYDICTPPQSAYDLKQA
                     FPEADLRMVIGGHSAFDDEVNKGLVAATDEFASR"
     gene            complement(3028..3414)
                     /locus_tag="PK856_04410"
     CDS             complement(3028..3414)
                     /locus_tag="PK856_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01649.1"
                     /translation="MNQKVWLAVVVATLWLSACRTTEMDNQRVMAPPETAQASSIHTA
                     IKQTVLKNTQRGWMVEASNAQEVVVGLHKRSHYLQVTYSLAGNKLESKITDSKNLNQE
                     GNQIHKSALRWKDQLDQQVFIAVSQL"
     gene            complement(3500..3778)
                     /locus_tag="PK856_04415"
     CDS             complement(3500..3778)
                     /locus_tag="PK856_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003682838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRL01650.1"
                     /translation="MALGHWSVYLILCENGALYCGISNDVDKRWKAHCTGKGAKYTRM
                     HKPVQMKEIYTDLTASEARKLEYQTKQLSKAAKQLLWDEAMVLRGVPQ"
     gene            complement(3884..5461)
                     /locus_tag="PK856_04420"
     CDS             complement(3884..5461)
                     /locus_tag="PK856_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HRL01651.1"
                     /translation="MDLSWVLEPATLVGFFTLLVLEVVLGIDNLVFVAILANKVKPQH
                     RDKARVIGLGLAVVMRIGMLGAMAYIMTLEQALFTIASHPFSGKDLIMLGGGLFLLYK
                     ATSELHERLEGHAEYVDGGKKSRGHAAFGSVVAQIILLDMVFSIDAVITAAAMVEHIT
                     IAMAAVVVAMGLMIWASKPLTTFVNNHPTVVMLCLGFLLMIGFSLIAEGLGMKIPKGY
                     LYAAIGFSILIEIFNQVSTKNRKKNAFSARTWRARTAESVLGMMGIREAVLAKAGVLN
                     GDGSHFEDNEKLMIQSVLTLAERPILGVMTPRPEIERLDISQSKEQQCKQLEDTPFSR
                     LMVVGKAGIDEPLGYINKKDLLAQMLATGEFSIHKALKQPLVLPENSTVLNAMELFRA
                     KSADIALVVDEFGAILGMVTMKDLMETIAGDFPEEYEREEAPSIEMHTDQSFTVDGSL
                     EYSSLAQQIDLPMQDDESDFHTVAGLIMDELQAIPDVDDSVDYHQWRFTVIEKVGQRI
                     ERVKVTRIPDEEWFQED"
BASE COUNT         1418 a         1550 c         1217 g         1431 t
ORIGIN      
        1 tgaacatgcg atacggctca ttcacgcctt tggtaatcaa atcgtctacc aacacaccca
       61 agtaagcctg ctcacggtgc aagaccaaag cctcacgctc aaacacatat tgcgccgcat
      121 tcgcacccgc aagcaaacct tgcgccgccg cttcttcata acctgtggtg ccattgattt
      181 gccccgcaaa gaacaaacct tggatggttt tggtttccaa gctggctttc aaattgcgcg
      241 ggtcgaaata atcgtattca atcgcgtaac caggtcgcaa aatgtgggca ttttccaagc
      301 ccttcattga gcgcaccaaa gcgatttgga tatcaaacgg caaggaagtc gaaataccat
      361 tagggtagta ttcatgcgtg tccaagcctt ctggctctaa gaaaatctga tgcgactctt
      421 tgtcggcaaa acggttaatc ttgtcttcga tggatgggca atagcgtggg cccacgcctt
      481 ctatcttgcc tgtaaacatt gggctgcggt caaaaccgct gcgtatgatg tcatgggtgg
      541 tgctgttggt atgggtaatc cagcacgaca cttgacgcgg atgcaaatca cgcgaacccc
      601 gcactgaaaa cacaggcgta ggcgtgtcac ctggctgttc actcaaagcc gaaaaatcaa
      661 tgctgcggcc atcaatgcgt ggcggtgtac ccgttttcaa acgcccttgt ggcaattgca
      721 attcgcgcag cttctcgccc aaactcatcg cagcaggatc gcctgcacgg ccgccttgat
      781 agttttccaa gccgatgtgg attttacccg acaagaacgt acccgctgtc aacaccaccg
      841 cacgagcatt gaattgcaaa cccatggtcg tgagcacacc gctgatgcgc tcaccgtcca
      901 aaatcacatc gtccacttgc tgctggaaca aatccaaatt ttcttggttt tccagcatgt
      961 ggcgtatcgc tgccttatac aacacacggt cggcctgtgc acgcgtggca cgcaccgctg
     1021 ggcctttaga cgcgtttaaa gtgcgaaact gtatgccgcc catgtcggca gccaaagcca
     1081 tcgcgccgcc caaagcgtcc aattcacgca ccaaatggcc tttgcctatg ccgcctatcg
     1141 acggattaca agacatctga cccaaagttt caatgttgtg gctcaataac agggtttttg
     1201 cacccatgcg cgcggcagcc aaagccgctt ccgtacctgc atgaccgccg ccaacgacaa
     1261 tcacgtcata aagagtgggg taaatcatcg tcccaagttt cgcaaacaaa atgggcttga
     1321 ttgtacgcca aactcatggc gctgtcagaa tttatcgcat cgcaacaatg caaatcgacc
     1381 gtgctttcaa gcagttgctg tattgccttt acgactactt tgcttacctc ctaaaagcgg
     1441 cctgtaaacc cagtccacga ccccttctcg atataaatca tgtcatatga atcataagca
     1501 aagttcacgc cttgattttc ctcacattcc actcgtaaga tacaccgatg attttttaca
     1561 ggccgctgcc atgacaccca ccacccgctt gctgcaaagc gatgatttcg ccgcttggcg
     1621 accactgtgg caacagtatt tggattttta ccaaaccgaa ttagacgaaa ccgtcaccct
     1681 ccacacatgg caaagactca tagacccaaa acaaaccgac atgctcggtt tgggtgtgtt
     1741 tgtggatggt cgcttgtcag gctttaccca tttggtgttt caccccaaca catggagcgc
     1801 cgagccctgt tgttatctcg aagacttgtg tgtggatgcg caagtgcgcg gccaaggctt
     1861 gggccgggca ttgatacagg ccgcccaaga tttggcccaa caaaaacagt gttgccgcct
     1921 gtattgggtc acagcccgcg acaaccacac cgcccaactc ctgtacaaca cactggccga
     1981 acaaaccgat ttcatccaat acaaaatcgg cctgtaaacc ctaattcaag gtttacaggc
     2041 cgcttattgc cagatttgct ggcaatacca cttatctagc gacttgcaaa ttcgtctgtg
     2101 gcagccacca agcctttatt cacctcatcg tcaaaagcgg aatggccgcc gatgaccatg
     2161 cgcaaatcgg cttcgggaaa ggcttgtttc aaatcataag ccgattgtgg tggggtgcaa
     2221 atgtcgtagc gaccttgcac gatgacggtg gggatgtggc ggattttgcc tatgttgctg
     2281 agcaaagcac gctcgccttg caaccagcct tggttgatga agtagtgatt ttccatgctg
     2341 gaaatcgcca aggcatgtgg gtcgttgtct tgtgggattt gcgggtgtaa gaacacgatt
     2401 ttttcttcgt attcacacca gtgacgagcg gcctgtattt tggtggtggc atccacgtct
     2461 ttgccaaaca acaaatggtt gtaagccgca atcaaatcac cacgctgcgc ttcaggaatg
     2521 aagttttcaa acgtctccca cgcttcaggg aaaatctgtg aagcaccgcc acgcttgaac
     2581 aaccaatcgc tttcaaattg gcggaacaag aatatgccgc gcaacaccaa ttcggtcact
     2641 cgttcagggt gggtttgggc ataggccaaa gacaaggtgc tgccccacga accgccaaac
     2701 accatccatt tgtctatgcc caaatgttcg cgcaagactt ctatgtcggc gaccaatttc
     2761 caagtgtcat tatcctctat gcatgcgtgt ggcaaggatt tgccacaacc gcgttggtcg
     2821 atttggatga tgcggtattt ttcaggattg aaaaacccac gcgtggcagg tgatgagccg
     2881 cctcctgggc cgccatgcaa aaagataacg ggcttaccat tgggattgcc acaatcttcc
     2941 caatacattt gatggatggc ggaaacttgc aacaaaccgc tttgattcac actgtctact
     3001 gtgtacattt tttgcccttt tgatggctta caattgggat acagcaatga aaacctgttg
     3061 gtccagttga tccttccatc gcaatgccga tttatggatt tgattgccct cttggttcag
     3121 atttttactg tcggtgattt ttgattccag cttattgcca gccaatgaat acgtcacttg
     3181 caggtaatgg cttcttttat gcaagcccac cacaacttct tgtgcattag acgcttccac
     3241 catccagcct cgttgggtat ttttcaatac agtttgcttg atggcagtat gtattgaaga
     3301 agcctgtgcg gtttctggtg gtgccatcac ccgttgattg tccatttcag tcgttcgaca
     3361 cgcagacaac cacaacgtgg ccaccaccac tgccaaccaa accttttgat tcatgccgcc
     3421 cccccttaaa atacaatgct acccgcaggt tcattgttca ataaaaacaa tccatattat
     3481 tatttcatta ccacagccat cactgcggta cgcccctcaa gaccatggcc tcatcccaca
     3541 acaattgttt ggcagcctta ctgagttgct tggtctgata ttccaatttt cgcgcttcgc
     3601 tggcggtcaa atcggtgtaa atttctttca tttgcactgg cttgtgcatg cgtgtgtatt
     3661 tggcgccttt gcctgtgcaa tgcgccttcc agcgtttgtc cacatcattg ctgatgccac
     3721 aatacaaagc gccgttttcg cacaatatca aatacacact ccagtggccc aatgccatcg
     3781 tgttgttgtt tatgtcatcc atcacagcag tattgccata ttcaagcggc ctgtaaacct
     3841 catcacagac caataaagca gcttgcgctg ctttgtttcc atctcaatcc tcttgaaacc
     3901 actcttcatc gggaatgcgt gtcaccttca ctcgctcaat gcgctgccct actttttcaa
     3961 tcacggtaaa acgccattgg tggtaatcaa cgctgtcatc cacatcgggt atcgcctgca
     4021 attcgtccat gattaaacct gcgacagtat ggaaatctga ctcatcgtct tgcatgggca
     4081 aatcaatttg ttgcgccaag ctgctgtatt ccaagctgcc atccacagtg aaactttgat
     4141 cggtatgcat ttcaatgctc ggtgcttctt cccgttcgta ttcttctggg aaatcgcctg
     4201 caatggtttc catcaagtct ttcatggtga ccatgcccaa aatcgcacca aactcatcca
     4261 ccaccaaggc aatatcagca ctcttggcgc ggaacaactc catcgcattc aatactgtag
     4321 aattttcagg caataccaaa ggctgtttca aggctttgtg tatgctgaat tcgcctgtgg
     4381 ccagcatttg tgccaacaaa tcttttttgt tgatgtaacc caagggctcg tcaatcccag
     4441 ccttgcccac caccatcaag cgtgaaaacg gtgtgtcttc caattgtttg cactgctgct
     4501 cttttgattg ggaaatgtcc aagcgctcaa tttctgggcg tggtgtcatc acacccaaaa
     4561 taggccgctc agccaaggtg agcacgcttt gtatcatcaa tttttcattg tcttcaaaat
     4621 ggctgccatc gccattgagc acacccgctt tggccaacac cgcctcgcgt atgcccatca
     4681 tgcccaatac cgattcagca gtacgcgccc gccatgtacg tgccgaaaat gcattttttt
     4741 tgcggttttt ggtcgacact tggttgaata tctcaatcag aatggagaag ccgatggctg
     4801 catacaaata gcctttagga attttcatac ccaagccttc ggctatcaaa ctgaaaccaa
     4861 tcatcaacaa gaaacccaag cacaacatca ccacggtagg gtggttgttc acaaacgtgg
     4921 ttaaaggttt cgatgcccaa atcatcaagc ccatggccac caccaccgcc gccatggcga
     4981 tggtgatgtg ttccaccatc gccgcagcag tgataactgc atcaatggaa aacaccatgt
     5041 ccaataagat gatttgcgcc accaccgaac caaaagcagc gtgtccacgg ctttttttac
     5101 cgccatccac atactctgca tggccttcca agcgctcgtg caactcacta gtggccttgt
     5161 acaagaggaa caagccaccg cctaacatga tcaagtcttt accagaaaag gggtgggatg
     5221 caatcgtgaa cagagcctgt tccaaggtca tgatgtaggc cattgccccc aacatgccaa
     5281 tgcgcatgac caccgccagg cccaaaccaa tgactcgggc tttgtcacgg tgttgcggct
     5341 taaccttatt ggccaaaatg gccacaaaca ccaaattgtc tatgcccagt accacttcca
     5401 acaccaacag ggtgaaaaag ccaaccaagg tggctggttc caatacccaa cttaaatcca
     5461 tgaaattatt ccaatttaac aaacgcaaac aggcgtcctg taaacacagg acgcccataa
     5521 acttgagagg gtcaacaaga tgaaggtctg tcgtgtcgga taatgactcg aaccatcttc
     5581 attcatactg ttgccgcctc cactaaaata ttgatg
//
LOCUS       DAOMVK010000091         5613 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_3970_length_5613_cov_2.951601, whole genome shotgun sequence.
ACCESSION   DAOMVK010000091 DAOMVK010000000
VERSION     DAOMVK010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3970_length_5613_cov_2.951601"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="PK856_04425"
     CDS             complement(<1..1049)
                     /locus_tag="PK856_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRL01652.1"
                     /translation="MNLDALWMPFSANKTFKKNPRILTSAKGMYFTDDKGRQILDGCA
                     GLWCVNAGHGNELIANAIGEQAKKLDYAPFFNMSHDIGFQAAERLVEMAPKGFDKVFF
                     TNSGSESADTSLKIALAYHAARGENGRIKFIGREKGYHGVNFGGTSVGGIGGNRKAFN
                     HLLPFVDHLRHTLDIERNAFTKGLPAYGAEWADELENSVLSLHDPKTVAAVIIEPMAG
                     SAGVILPPQGYLQRIRDICTKHGILLIFDEVITGFGRTGGDWATNTMGVSPDILNCAK
                     GLTNGTVPMGAVLVGNDIYNTVVDAAPEGAIEFAHGFTYSAHPLACAAAIATLDVYRD
                     EGLFERAAAMAPKLE"
     gene            1203..2567
                     /locus_tag="PK856_04430"
     CDS             1203..2567
                     /locus_tag="PK856_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRL01653.1"
                     /translation="MKTLVEKLMQTIGAQIERGQLPQGSKLPSIRQAAKLYGVSTFTV
                     AEAYSRLCTQNQLESSAGRGYFVAKPSYQQPLETVLRQPLNQNWLLQGIYQSDDKMLQ
                     AGCGWLPDDWHDPELASSALRKLARAPLQLNQYGEPKGLLPLRQLLAQRWAHHHLPLS
                     ANQVLLTQGASQALDIITATYCQAGDTVFIDNPGYTNLLTRLRSKQLHIVGVPWNDQG
                     PDVVQLAQLLQQYRPKLMFTNPVLQNPTSANYSLATAHQVLHLLREASCLLVENQVSY
                     WLAHNPPPPLSALDSIQHSLLIGSFTKHLSPVLRVGYVLGTDERIAALMQQKMLSGMT
                     TSAINEHLALLMWQDSRSDKQLSQWRNRLAGAQQHLQKVFSGLGWQVFGRPQAGMYLY
                     AQHPAIDDAYQFAQTHHDDNITLAPGLLFAIDQSQANPWLRFNVAYCMRPEFEPWLHL
                     VTPK"
     gene            2862..3731
                     /locus_tag="PK856_04435"
     CDS             2862..3731
                     /locus_tag="PK856_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01654.1"
                     /translation="MNTTLKRGLQILGTIAILGTSACVVDPYSSGVGYGSYDGYDYDN
                     SYTSTTRVIGNGSNFAFNTGFLVGNYYYYDDNYYPVNYYTSGGTRYYRPVFDRPYHIH
                     PDSRYRNLNRNQLEQWRRNQSIRYDPSYRPNHWNNGNRDHWNNRDKNRWDNNRGNNRW
                     NNGRDDKRWDNRRPIISQPSNPNNRPNQHWNRPQVQPPRDNNMTRPSVRPTPSIPTTR
                     PNTQVTRPTVVQPRPQVQPIRSVPNNRPIMQNRSSVQSNDTRSLMQNSRSQMQMQPRL
                     DNSNMGNRRMHER"
     gene            3795..4022
                     /locus_tag="PK856_04440"
     CDS             3795..4022
                     /locus_tag="PK856_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003793953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HRL01655.1"
                     /translation="MNTIPRGIYQHYKGQQYEVLGCAHHSETEEVLVVYRALYGDYGL
                     WVRPLAMFSGLVLIDGEQLPRFRLIHAHEHS"
     gene            complement(4090..5268)
                     /locus_tag="PK856_04445"
     CDS             complement(4090..5268)
                     /locus_tag="PK856_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HRL01656.1"
                     /translation="MKKTLLASSVVLMLLAACGGGNNNAAQTKTSDAGATASSGSNEA
                     AAPVAAATDSLKIYNWSDYVDPETVKEFEAANKIKATYDFYDSNEALEAKVLTGKSGY
                     DLVVPSNTFVGRQIKAGAYLKIDKNQIPNYNNIDPQLLKLMNDVDAGNEFAVPYFWGI
                     NTLAINKDKVEKALGGPLPDNQWDLLFKPEYSNKLKSCGISILDSAAEVFPMALNYMG
                     KNPQSDKSEDLKAAAEMIMKVRPDIRRFSSSGYIDDMARGDLCVVLGYGGDLNIAARR
                     AKEAKSNVNIEVLVPKEGFGVWIDSFMIPKDAANVTNAYKYINNTLDPKVAAKNGNFV
                     TYAPASKPAKDLMDKQYTENRSIFPTDADLQNGFMMTPMKPDSLKMAVRLWQDIKKGS
                     "
BASE COUNT         1482 a         1492 c         1198 g         1441 t
ORIGIN      
        1 tgttccaatt taggcgccat ggcagcagca cgctcaaaca aaccttcgtc gcggtacaca
       61 tccaaagtag cgatggcagc agcacaagcc aatgggtgtg ctgagtaagt aaagccatga
      121 gcaaactcaa tcgcaccttc aggtgccgca tctactacag tgttgtaaat gtcattgccc
      181 accaacacag cacccatagg cacagtaccg ttggtcaagc ccttggcaca attcaagatg
      241 tctggagaca cacccatggt gttggtggcc cagtcaccgc ccgtacgacc aaagccagtg
      301 atcacttcat caaaaatcaa caaaatgccg tgtttggtac aaatgtcgcg tatgcgttgc
      361 aaatagcctt gtggaggcaa aatcacgccc gcagagcccg ccataggttc gatgatgacc
      421 gcagcaacgg ttttcggatc gtgcaagctc aacacgctgt tttccaactc atcggcccat
      481 tcagcgccat aagcaggcaa acctttggtg aaggcattgc gttcgatgtc caaggtgtga
      541 cgcaagtgat ccacaaatgg caacaagtgg ttaaaggctt tgcggttacc gccgatgcca
      601 cccacagaag tgccaccgaa gttcacacca tgatagcctt tttcacgacc aatgaatttg
      661 atgcgaccgt tttcaccacg ggcagcgtgg taagccaagg caattttcaa tgaggtatca
      721 gcagactcag aaccagagtt ggtgaagaac accttgtcaa aacctttagg cgccatttca
      781 accaaacgct cagcggcttg gaaaccgatg tcatggctca tattgaagaa aggagcatag
      841 tccaattttt tggcttgttc accaatggca ttggcaatca attcgttacc gtgacctgca
      901 ttcacacacc acaaaccagc acaaccatcc aaaatttgac ggcctttgtc atcggtgaaa
      961 tacatgcctt tggccgaagt caaaatgcgt gggtttttct tgaaggtttt gttggccgaa
     1021 aacggcatcc acaaagcatc taaattcatt gtcattctcc tcattttaat gatgcgtcgt
     1081 taatgctgcc actttaacag cagcagaata aaaaaacagt gacacttcga ccgatttccg
     1141 acttactgta taggccatat ttcctgtaca gttacatttc cccacctttt acaggccgct
     1201 tgatgaaaac cttggtagaa aagctgatgc aaaccatagg cgcgcaaatt gaacgcggtc
     1261 aattgccgca gggcagcaaa ttgccctcca tccgtcaggc ggcaaaactg tacggtgtca
     1321 gcaccttcac cgtcgcagaa gcctattccc gcttgtgcac ccaaaatcaa ttggaatctt
     1381 cagcaggccg aggttatttt gttgccaagc ccagttacca acaaccgctt gaaaccgtac
     1441 tgcgccagcc cttgaatcaa aactggctgt tacaaggcat ttaccaaagt gatgacaaaa
     1501 tgttacaggc cggttgtggt tggctgcccg atgattggca cgaccctgag cttgccagct
     1561 cggcactgcg taaactggcg cgtgcgccct tacagttaaa ccaatatggc gaacccaaag
     1621 gcttattgcc tttgcgccaa ttgctggcac agcgctgggc acaccaccat ctgccccttt
     1681 ctgccaatca agttttgctc actcaagggg ccagtcaggc cttggacatc atcaccgcca
     1741 cctattgcca agcgggcgac accgtgttca tagacaatcc aggttacacc aatttgctca
     1801 cccgcttgcg cagcaaacag ctgcacattg tgggtgtgcc ttggaatgac caaggccctg
     1861 atgtggtgca gttggcgcaa ttgttacagc aataccgacc taaattgatg ttcaccaacc
     1921 ccgtgttgca aaaccccacc agcgcgaact acagcctcgc caccgcccat caagtattac
     1981 acctgctgcg agaagccagt tgtttgttgg ttgaaaatca agtgtcgtat tggttggctc
     2041 acaatccccc acccccattg tcggcattgg acagcataca acacagtttg ctcataggca
     2101 gttttaccaa acacctgtct cccgtcttgc gtgtgggtta tgtattgggc acggatgagc
     2161 gcatagcggc tttgatgcaa caaaaaatgc tcagcggcat gaccacctcc gccatcaatg
     2221 aacacttggc tttgctgatg tggcaagaca gccgcagcga caagcaattg tcacaatggc
     2281 gcaaccgctt ggctggcgca caacaacacc tgcaaaaagt gttcagtggc ttgggttggc
     2341 aagtgtttgg ccgccctcaa gctggcatgt atttgtatgc ccaacacccc gccatagacg
     2401 atgcctacca attcgctcaa acccaccacg atgacaacat cacgcttgcc ccaggcttgc
     2461 tgtttgccat agaccaatcc caagccaacc cttggctgcg cttcaatgtg gcctattgca
     2521 tgcggccaga atttgagcct tggttgcact tggtcacacc caaataactc tgtttgctcc
     2581 acttgtttta aatccttgtc actcttttac atcaatgaag cggcctgtaa acctttacag
     2641 gccgcttcaa cagtgaatcc acccaaggtt ttagatacca cttccagtcg aataaacaac
     2701 aatcgtgtca ggtttgtttc aaactaagta aattcttgcg ggtttttgca gtcaaggcac
     2761 agagactgct atcattaatg cgtctgtcag gacaccacac acacatggcg gttcgggctt
     2821 aaccagcctc atgtgtgtgc catcactgga aagaacacac aatgaacacg accttaaaac
     2881 gcggcttaca aatactgggc acaatagcca tcttgggcac cagtgcctgt gtggttgacc
     2941 cttattcttc tggcgtgggt tatggcagtt acgatggtta tgactacgac aacagctaca
     3001 cctccacgac ccgtgtcatc ggcaatggca gcaattttgc ctttaataca ggcttcttgg
     3061 tgggtaatta ttactattac gacgacaatt actaccccgt taattattac acttcaggtg
     3121 gcacacgcta ttaccgccct gttttcgacc gcccttatca cattcatccc gattcccgct
     3181 atcgcaacct caatcgcaat cagctagagc aatggcgtcg caatcaatcc atacgttatg
     3241 acccttccta tcgccctaat cactggaaca atggcaaccg tgaccattgg aacaatcgcg
     3301 ataaaaaccg ctgggacaat aaccgtggca acaatcgttg gaacaatgga cgtgatgaca
     3361 agcgttggga taaccgacgc ccgatcatct cgcaaccttc caatccaaac aaccgcccaa
     3421 atcagcattg gaaccgtccg caggtacaac ctccgcgcga caacaacatg acccgtccat
     3481 cggtaaggcc aactccaagc attcctacca cgagaccaaa cacccaagtt acacggccaa
     3541 ccgtggttca gccacgacca caagtgcaac caatccgttc tgtaccgaac aatcgcccta
     3601 tcatgcaaaa tcgctctagt gttcaaagca atgacacacg cagcttgatg caaaactcac
     3661 gcagtcaaat gcaaatgcaa ccgcgtttag acaatagcaa tatgggcaac cgccgcatgc
     3721 acgagcgcta aacacataac ctacaacaag cgttctttac aggacgcttt ttttcttgat
     3781 ttgagcaaac acacatgaac actatcccca gaggcatata ccaacattac aaaggccaac
     3841 aatacgaagt gttgggctgc gcgcaccatt ccgaaaccga ggaagtctta gtggtttacc
     3901 gcgccttata cggtgattat ggtttgtggg taaggccact ggcgatgttt agcggcttgg
     3961 tattgattga tggcgaacaa ttaccacgct ttcgtttgat ccatgctcat gaacacagct
     4021 aggttcactg tctattggtc ttaaatgtca aaaaacccgc tatgagcggg ttttttatca
     4081 actgaatgtt tagctgcctt ttttgatgtc ttgccacaaa cgcactgcca tcttcaagct
     4141 gtctggtttc attggtgtca tcatgaagcc attttgcaaa tcggcatcgg ttgggaaaat
     4201 cgagcggttt tccgtgtatt gcttgtccat caagtctttg gcgggtttac tggctggcgc
     4261 ataagtcaca aagttaccat ttttcgccgc cactttagga tctaaagtgt tgttgatgta
     4321 tttgtaagca ttggtcacat tggcagcgtc ttttggaatc atgaacgaat caatccacac
     4381 accgaagcct tctttaggca ccaaaacctc aatgtttacg ttgcttttcg cttctttggc
     4441 acggcgggcg gcaatgttca aatcaccacc ataacccaac accacgcaca aatcgccacg
     4501 ggccatgtca tcaatgtaac ccgatgagct gaaacggcgt atgtcaggac gcactttcat
     4561 gatcatctct gccgctgctt tcaaatcctc gctcttgtca ctttgtgggt ttttacccat
     4621 gtagttcaaa gccataggga acacttccgc agcactgtcc aaaatactga tgccacaaga
     4681 tttcagcttg tttgagtatt ctggcttaaa caacaaatcc cattgattgt ctggcaatgg
     4741 gccgcccaag gctttttcta ccttgtcttt gttgatggca agcgtgttaa tgccccagaa
     4801 atatggcaca gcaaattcat tgcccgcgtc cacatcattc atcagtttca acaattgtgg
     4861 gtctatgttg ttgtaattgg ggatttggtt tttatcgatt ttcaaataag caccggcttt
     4921 gatttgacga cccacaaaag tattgcttgg caccaccaaa tcatagcccg acttgcctgt
     4981 taacactttg gcttccaaag cctcgttgct gtcgtaaaaa tcgtaagtgg ctttgatttt
     5041 attggccgct tcaaattctt tcacggtttc tgggtcaaca taatccgacc aattgtaaat
     5101 tttcaaactg tcggtggccg cagcaacagg tgcagctgcc tcgtttgaac cactgctggc
     5161 agttgcacca gcatctgaag tttttgtttg ggctgcattg ttattgccac cgccacaagc
     5221 ggctaaaagc ataagaacca cagaagatgc cagaagtgtt tttttcataa tgttttctcg
     5281 ataattcaat caaccttgca catgtgggtg tttgatgggg tgcaagacac gaaatattgt
     5341 gttgacgatg ctattgccaa ccgcgtttat taaactgtgt tttttatgac atgacaagtt
     5401 atttagtcat ctttgctgca caaaaataca cgggcaaaat aggtaaaatc acaacgactc
     5461 aatccaactc agcatgctta ttcctatgta ttttatttca taacatattt acaggccgct
     5521 gctattttaa gcacagaaaa acccgcgctg tgcgggcttt tctcatcatg gtttcaattg
     5581 ccttttttga tgtcttgcca caagcgtgtg ctc
//
LOCUS       DAOMVK010000092         5599 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_3979_length_5599_cov_3.049423, whole genome shotgun sequence.
ACCESSION   DAOMVK010000092 DAOMVK010000000
VERSION     DAOMVK010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3979_length_5599_cov_3.049423"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="PK856_04450"
     CDS             complement(<1..758)
                     /locus_tag="PK856_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HRL01657.1"
                     /translation="MHTLKHIKAQTALKYALVLLLLLAFSLSFYHSNAWLQLCYGLAI
                     FLFGMQCIEEGLQRAAGGYLERLLNKSTDTPFKGLLFGMGATFVLQSSTLVSLLTIAF
                     LSAGLIGLGAGIAVLLGTNLGATSGIWLLALAGQNISLSVVALPLLFFGVLASFFGDT
                     GKAFGRALMGIGFIFVGIDAIKLGFTDMGGAVDFTQLHVSGITEILIFTAIGLLLTVV
                     LQSTHAALILTLAALAGGQIVPEQAFALAIGSNVG"
     gene            complement(846..2276)
                     /locus_tag="PK856_04455"
     CDS             complement(846..2276)
                     /locus_tag="PK856_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRL01658.1"
                     /translation="MSRKNRPYHFYDTTGSICSTCFRQIEAKILFKDGKVYMDKWCPQ
                     HGTERVLISDDETYYRLGREVFVKRPEMPQTFNTPMHYGCPYDCGLCPDHMQHSCLSI
                     IEINEACNLNCPVCFAASGTHKHIHYPLDKIEAMLDTVVKNEGEPDVVQLSGGEPTIH
                     PQFFEILAAARARPIKHLMINTNGIRIAQDPEFVAKLAEFGKHGLEIYLQFDSMRPEV
                     LKTLRGADLSRIRRQALENLNQHNLSTTLVVTVARGHNDDELGDIIRFAQSQPCVRGV
                     TIQPIQQAGRQTGVRADNRLTVSEIRRNIIQQSGIFNEADIVPVPCNPDTLAMAYALK
                     LGDSLTPLTRFFEPQTLVEGSANTIVFEHDVDLKQQVFKLFSTNHSPESQANCLSELM
                     CCLPQIAAPNLGYENVFRILIVQFMDATNLDIRALKKSCIHFATADGRMIPFESHNLF
                     YRPEQAALLQGLRDKIEQTHVVWQEV"
     gene            complement(2413..2757)
                     /locus_tag="PK856_04460"
     CDS             complement(2413..2757)
                     /locus_tag="PK856_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01659.1"
                     /translation="MTQDQTPQQTPTPNPSWTVFVMDVWLVFGVLSLLLAVWFIPGES
                     DWRALGWLILDFGLMFLFSALLLRWQLPKTHTPNHQLSALVLVLFLYAPAFYLACVSC
                     FCGHRQRCLARS"
     gene            complement(2861..3145)
                     /locus_tag="PK856_04465"
     CDS             complement(2861..3145)
                     /locus_tag="PK856_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01660.1"
                     /translation="MNNPYQTPKKKLPQSDQKRLLSNGQFWLKIIGLVILALLFLPAL
                     FYGLCFLVLSTGMNGYEFMLGGFIGLVVAACIAYGFVKLLRWRGDDVGED"
     gene            complement(3214..3495)
                     /locus_tag="PK856_04470"
     CDS             complement(3214..3495)
                     /locus_tag="PK856_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01661.1"
                     /translation="MQEEHPQNQENSAPSQETSAAPPVHTRYDRNTNGTNGCLVFFLI
                     IAAMILLLPVVFFGFCMLMSVNDTKSQLIGLGILVIVAIIVWSVFVSLR"
     gene            complement(3888..4031)
                     /locus_tag="PK856_04475"
     CDS             complement(3888..4031)
                     /locus_tag="PK856_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01662.1"
                     /translation="MHSEPQQPDTETPSEKSPKFKNTKQSNGCLIAAFIVAGLVWGSI
                     QSS"
     gene            complement(4091..4876)
                     /locus_tag="PK856_04480"
     CDS             complement(4091..4876)
                     /locus_tag="PK856_04480"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003793855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRL01663.1"
                     /translation="MLEALIIQDPTVARIVHSSLELLALSGGFMLYRYLKRRKGDTTS
                     LWHGQGIWVVLGCLIGAGIGNKLVSALHHPVLMQSLWQQSPQAFFVALFSGQSVVGGL
                     LGGWIGVELGKKYAGITTRTGDDFVVPILFGLVVGRLGCFLAGAYDGTWGTHSNLPWA
                     VDFGDGPRHPAALYEMLLALLALASYPWWKQSLQAGLGLRFRALMVAYLVWRLLIDGL
                     KPIPEGWPLGMAGIQWVCVVALGVMTVCEIKKLLTKGNAQVSS"
     gene            5029..5469
                     /locus_tag="PK856_04485"
     CDS             5029..5469
                     /locus_tag="PK856_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01664.1"
                     /translation="MLGHGIVAKVNGVRNPMKRLNLLLSAHAAITFVAACVLVWFPSV
                     IPALVGVTLTEGQYLICYLLAAFELSMAYLSWQSRHINDPHALSVIVKTIILLHGSSA
                     VLELDVALQTMSLALMVNIVARLIVCGAFYCFGLRVLRLRGGTE"
     gene            complement(5449..>5599)
                     /locus_tag="PK856_04490"
     CDS             complement(5449..>5599)
                     /locus_tag="PK856_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HRL01665.1"
                     /translation="DTEEAVIKEVARLEKAMFAASRDLQFEEAAELRDQIRALKENLL
                     FGAAP"
BASE COUNT         1471 a         1333 c         1445 g         1350 t
ORIGIN      
        1 cccacattcg agcctatggc caaggcaaac gcttgctcag gcacgatttg accgcctgcc
       61 aaggccgcca aagtcaaaat caaggccgca tgggtggatt gcaataccac ggttaagagc
      121 aaaccaatgg cggtgaatat caggatttcg gtgatgccgc tgacgtgtaa ttgggtgaaa
      181 tcgaccgcgc cgcccatgtc ggtaaagccc agtttgatgg catctatgcc cacgaaaata
      241 aagcctatgc ccatcaatgc gcggccaaac gccttgcctg tatcaccaaa aaaactcgcc
      301 aacaccccaa aaaacagcaa aggcaaagcc accacgctca agctgatgtt ttgtcccgcc
      361 aacgcgagca gccaaatgcc actggtggcg cccaaattgg tgcccaacaa caccgcaatg
      421 cccgcgccca agccaatcaa acccgcgctt aaaaacgcaa tcgtgagcag cgacaccaaa
      481 gtgctcgatt gcaacacaaa cgtcgcgccc atgccaaaca gaaggccttt aaacggcgtg
      541 tcggtagact tgttcagcaa gcgctccaaa taaccgcccg ccgcgcgttg caaaccttct
      601 tctatgcatt gcatgccaaa caagaatatc gccaagccat aacacaattg cagccacgca
      661 ttgctgtggt aaaacgacag gctaaaagcc aatagcagca gcaagaccag tgcgtatttg
      721 agggcggttt gcgctttgat gtgtttgagc gtgtgcataa aactccttgt aacagtcata
      781 cagcggcctg taagccgctg tatggaaaaa tcattgtcat ctgcgtgaca tgtgccacat
      841 catagtcaaa cttcctgcca cacgacatgc gtttgctcaa ttttgtcgcg caagccttgc
      901 agtaaagcgg cttgttcggg gcggtagaag aggttgtggc tttcaaatgg aatcatgcgg
      961 ccatcggcgg tggcgaaatg gatgcacgat tttttcaggg cgcggatgtc gaggttggtg
     1021 gcgtccatga attgcacgat taagatgcga aacacgtttt catagcccaa attgggcgcg
     1081 gcaatttgcg gcaagcaaca catcaattcg ctcaagcaat tggcttggga ttcggggctg
     1141 tggttggtgg agaataattt gaacacttgc tgcttcaaat cgacatcgtg ttcaaacacg
     1201 atggtgttgg cgctgccttc taccaaggtt tgtggctcaa aaaagcgggt gagtggagtg
     1261 agactgtcgc ccagttttaa ggcgtaagcc atggccaagg tgtcgggatt gcacggcacg
     1321 ggcacaatgt cggcctcatt gaaaatgccc gattgctgaa tgatgttgcg gcggatttca
     1381 ctgacggtca aacggttgtc ggcgcgcaca cctgtttgac gccccgcttg ctgtatcggc
     1441 tgtatggtca cgccgcgcac gcaaggctgg ctttgggcaa agcggatgat gtcgcccaac
     1501 tcatcatcgt tgtgaccgcg tgctaccgtc accaccaagg tggtggacag attgtgttgg
     1561 tttaaatttt ccaatgcttg gcggcggatg cggctcaaat cggcgccgcg cagcgttttc
     1621 aacacttcag gccgcatcga atcaaattgc agatagattt ccaaaccatg tttgccaaac
     1681 tcggccagtt tggcaacaaa ctcagggtct tgcgcgatgc ggatgccatt ggtgttgatc
     1741 atcaaatgct tgatggggcg cgctcgcgcg gcggccaata tttcaaaaaa ctgcggatgg
     1801 atggtgggct cgccgccaga taattgcacc acatcgggtt cgccttcatt ttttaccacc
     1861 gtgtccaaca tggcttcaat cttgtccaat gggtaatgaa tgtgtttgtg ggtgccgctg
     1921 gcggcaaaac acacggggca attgaggttg caagcctcgt tgatttcaat gatggaaaga
     1981 caagagtgct gcatgtggtc ggggcagagg ccgcaatcat acgggcagcc atagtgcatg
     2041 ggcgtgttaa aggtttgggg catttcaggc cgcttcacaa acacttcgcg ccccaatcgg
     2101 taataggttt catcgtcact gatgagcacc cgctcggtac cgtgttgtgg gcaccatttg
     2161 tccatgtaca ccttgccgtc tttgaacaaa attttcgctt cgatttgtcg aaaacaagtg
     2221 gagcaaatgc tgccagtggt gtcataaaaa tggtaagggc ggtttttgcg cgacatagaa
     2281 agcgtcctgt aaacaattat ttggaggtgt ggcgaagctt gtggcgatac agccaataag
     2341 cgcaagcaaa atcgctgcac aataaagcca taaacagtca atgttggtat tgcatgacag
     2401 taatttgtat tgtcatgagc gagcgagaca gcgttggcgg tgtccacaaa agcaggaaac
     2461 acaagccaga taaaaggctg gtgcgtaaag aaacaatacc aaaaccagcg cacttaattg
     2521 atggttagga gtatgggttt taggcagttg ccaacgcaat agcagtgcac tgaacagaaa
     2581 catgaggcca aaatccaata tgagccagcc caacgctcgc cagtcgcttt caccaggaat
     2641 aaaccacact gccaacagca gactgagcac accaaatacc aaccaaacgt ccatgacaaa
     2701 tactgtccaa gaggggtttg gcgtgggtgt ttgttgaggt gtttggtctt gggtcatgta
     2761 tttttctgtt ttaattgggt taagtgatgg gtttgctgtg ccaaaatgga tgtaagcggc
     2821 ctgtaaatca aatcacccac aagcactgtt tgttgctgat tcagtcctca cccacatcat
     2881 cccctcgcca acgcaacaat ttaacaaaac cataagcgat acacgctgcc acgaccaaac
     2941 caatgaagcc accgagcatg aattcatagc cattcatgcc cgtggacaaa accaagaagc
     3001 acaagccgta aaacagagca ggtaggaaca gcagtgccaa aatgaccaag ccaatgattt
     3061 ttagccaaaa ttggccgttg cttaataggc gcttttggtc gctttgaggc agctttttct
     3121 tgggtgtttg gtaagggttg ttcatgaatt gatctcaaac acagttaatg aggggttaaa
     3181 gtaaggcatg gcctcacaaa atggtagcga aagtcagcga agagagacaa aaacagacca
     3241 cacaatgatg gccacgatta ccaatatgcc cagccctatc aactgtgatt ttgtgtcgtt
     3301 gactgacatg agcatacaaa aaccaaaaaa aaccaccggt aacagtaaaa tcatcgcagc
     3361 gattatcaag aaaaatacca agcatccatt tgtgccattg gtattgcggt cataacgagt
     3421 atgaacaggt ggtgcagcgc ttgtttcttg cgagggtgct gagttttctt gattttgcgg
     3481 gtgctcttct tgcataaacg tgtgttcctc aagtgatggg gcatgtcaac aaaatgtcag
     3541 atacatcagt gatgtttcat cagttgttgg tcgctttgag gcagtgtttg cttgggtgtt
     3601 tggtaggggt tgttcatggt tggggcctca agtgcaaatc aaaaagtgtt tggtcgtgct
     3661 aggtatgcat ggctctgtgt gcgcatcgtt acaaaatcgg ctgaatgcga tgaataccag
     3721 tgttatgagt attcaactgt atggctatac gcctgaagct gtttccacgt tttatgaacg
     3781 ctgatgctgc gagattcagg gcttatctta attcgttaat caggtacacc actccaataa
     3841 tgcttattac gatcactgca aacacgccta gaccaaaaga ctgatcacta ggatgactgg
     3901 atagaacccc acacaagccc agcaacaata aaggcggcaa tcaaacaacc attggattgt
     3961 ttggtgtttt tgaatttggg cgatttttct gatggggttt cggtatctgg ttgctgtggt
     4021 tcagaatgca tgtgtaatac ctcaaaataa acatgacaca gatgaaaaca taattgggtt
     4081 ggactgggtt ttaggagctg acctgagcat tgcccttagt cagcaatttt ttgatttcac
     4141 acactgtcat tacccctaaa gccaccacgc acacccactg tatgcctgcc atgcccagtg
     4201 gccagccttc gggtatgggc ttgaggccat caatcaacaa acgccatact aaataagcta
     4261 ccatcagcgc gcgaaagcgc aagcccaaac cggcctgtaa gctttgtttc caccatggat
     4321 agcttgccaa ggccagcaga gcaagcaaca tttcgtacaa ggcggcagga tggcgtgggc
     4381 catcgccaaa gtccacggcc caaggcaggt tgctgtgtgt gccccatgtg ccatcgtaag
     4441 cgccagcgag aaaacagccc aagcggccaa cgaccaagcc aaacaaaatc ggcaccacaa
     4501 aatcatcgcc agttcgggtg gtaatgcctg cgtatttttt gcccaactct acgcctatcc
     4561 agccaccgag caagccccca accacgcttt ggcctgaaaa caaggccacg aaaaaggctt
     4621 gtggtgattg ttgccacaaa gactgcatca acactgggtg gtgcagggca gagaccaatt
     4681 tattgcctat gcctgcaccg atgaggcagc ccaataccac ccagatgcct tggccatgcc
     4741 acaagctggt ggtgtcgccc ttgcgtcttt ttaaataacg gtagagcatg aagccaccgc
     4801 tgagcgccag taattccaag ctcgagtgaa caatgcgggc aacagttggg tcttggatga
     4861 tgagggcttc tagcatgact gactcaggga atgttttaga acatcatacc aataatgctg
     4921 tgggaatgtt tggaatcaat ttgaaacttg tctgcgtgca aatacggaca cgtgaaggcg
     4981 caaatattaa gcaagcggcc tgtaagcctt gggtgggtgt gggcattcat gttaggacat
     5041 ggcattgttg ccaaagtgaa tggagtgagg aatccgatga agagattgaa tctgttattg
     5101 agtgcgcacg cagcaatcac ttttgttgct gcgtgcgtat tggtgtggtt tcccagtgtg
     5161 atacctgcat tggtgggcgt gacactgact gagggccaat acctcatctg ctatttactg
     5221 gccgcttttg aattgagcat ggcttatttg tcgtggcaaa gcaggcacat caacgatcca
     5281 catgcactca gcgtcattgt caaaaccatc atcctcttgc atggcagttc tgcggtgttg
     5341 gagttggatg tggctttaca aacgatgagt ctggccttga tggtgaacat cgtggccaga
     5401 cttatcgttt gtggtgcgtt ttactgcttt ggactgcggg tattgcgctt aaggggcggc
     5461 accgaataag aggttttctt tcagggcgcg gatttggtcg cgcagctcgg cggcttcttc
     5521 aaattgcaaa tcgcgtgagg cggcaaacat ggctttttcc aagcgtgcca cttctttgat
     5581 cacggcttct tcggtgtcg
//
LOCUS       DAOMVK010000093         5585 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_3994_length_5585_cov_3.011573, whole genome shotgun sequence.
ACCESSION   DAOMVK010000093 DAOMVK010000000
VERSION     DAOMVK010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3994_length_5585_cov_3.011573"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="PK856_04495"
     CDS             <1..709
                     /locus_tag="PK856_04495"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HRL01666.1"
                     /translation="IESLESGDYKGLPEPIFIKGFIKTYARFLGLDIDQITVLLNEAF
                     PSQVTAIASTSPAAESQNGLNFQDQKKSKSFPKWLPLLVVAAAVMGAVYLWQSKSSEQ
                     TRQQEKSTLEVGDVAQPQLATDNVQVVPMNASATSASTPVNASAPMAASAAASGGIQI
                     KVRYRSNLIVTDADGTPLHSGIVASNSSHEFKGKAPYQVRIGYATGSEISFNGQPIDL
                     KNHFVDGKTVALTVPQP"
     gene            706..1971
                     /gene="ispG"
                     /locus_tag="PK856_04500"
                     /gene_synonym="gcpE"
     CDS             706..1971
                     /gene="ispG"
                     /locus_tag="PK856_04500"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HRL01667.1"
                     /translation="MREIKRRQTQQVQIGHVFVGSDHPVLVQSMTNTDTADAEGTALQ
                     VKALADAGSEVVRITVNSPEAACKVAEIRQRLDDMGCNTPLVGDFHFNGERLLRDYQD
                     CAKALAKYRINPGNVGKGAKGDEKFAYMIEQAALHDKAVRIGVNWGSLDQSLAKKMMD
                     ANLALAEPAPADAVMKEALITSALTSAQKAIDLGLPKNKIILSCKVSNVQDLIEVYGD
                     LAQRCDYPLHLGLTEAGMGSKGIVASSAALAVLLQRGIGDTIRISLTPEPGAPRTQEV
                     VVAQELLQTMGLRNFTPLVTACPGCGRTTSTVFQELARDIQHFLREQMAIWRLKYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGDHMAQEFI
                     AIVEEYVETNYGENGAKRRQNRVIPIQTR"
     gene            1986..3272
                     /gene="hisS"
                     /locus_tag="PK856_04505"
     CDS             1986..3272
                     /gene="hisS"
                     /locus_tag="PK856_04505"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HRL01668.1"
                     /translation="MGKKVQAVKGMNDLLPVVQKDWPITSAFWLAFEQRISTWAEQYA
                     YQFIRTPIIEPTGLFVRSIGEETDVVGKEMYTFDDYGESLSLRPEGTASCLRAVVEHN
                     LLYNGPMKLWYMGPMFRHEKPQKGRYRQFHQVGCEALGYSQPDVDAEMIVMTADLWKQ
                     LGILDKVELQINTLGVAEERAAHRAALVAYLQQHEAKLDEDSKRRMHTNPLRVLDTKN
                     PDLQDIANNAPRLMDFLGEESLAHHQAFKALLDSVGVAYVENPRLVRGLDYYNKTVFE
                     WVTSELGSQGTICGGGRYDGLIEELGGKEAPAIGFAMGIERLLLLVQQAGQLDVAANL
                     DVFVMYQGEGTMQQALTVATLLRQAGLKVSQYSGTQSLKAQMKKADGSGARFAVIIGE
                     EELAKDQVSLKDLRGDKGQLQAACSDLVQQLQQWMV"
     gene            3276..3896
                     /locus_tag="PK856_04510"
     CDS             3276..3896
                     /locus_tag="PK856_04510"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRL01669.1"
                     /translation="MAYNVEEQQDIDNFKHFWSKGGKWLFVLLLAAGLAYLGFVLSKS
                     YAKGQDEEAAALAYQFVEKSSKKDASALTDLKTLQEQHGKSFGAAEATLMAAAQAFDE
                     KKYDQAEKHLLWVQKNNPDVFIQALSNQRLAVMKLQQQQYDAALKFVDATVPAELKPM
                     MLETKGDILAAKGDKAQAKTAYAEALKGLDEKTSPNYELLKFKAEQ"
     gene            3985..5433
                     /gene="der"
                     /locus_tag="PK856_04515"
     CDS             3985..5433
                     /gene="der"
                     /locus_tag="PK856_04515"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRL01670.1"
                     /translation="MKPTIVLVGRPNVGKSTLFNRLTRSKDALVADLPGLTRDRHYGH
                     GRVGEKPYLVVDTGGFEPVVDSGILFEMAKQTLQAVDEADAVVFLVDARVGVTPQDKL
                     IAQKLRMSGRPVFLAVNKGEGTNRAVVAAEFYELGLAVEPFVISSAHGDGVWDLISAV
                     LEEFPDAVADEVATDHPVFAVIGRPNVGKSTLVNAILGEERVIAFDQAGTTRDSIHID
                     FERDGKPYTIIDTAGVRRRGKVDEAIEKFSVIKSMQAVEAANVAVLVLDANQDIADQD
                     ATVAGFALEAGRALVVAINKWDVVTPERKEQIKKDIARKLYFLDFAKFHYISALKHKG
                     IDDLFGSIHQAYNAAMIKMPTPKITRVLQSALERQQPPRAGLIRPKMRYAHQGGVNPP
                     IIVIHGNALHNIKDSYTRYLIQTFRKAFHLEGTPLRVQYKSSENPFEEKREQTSAQPL
                     RRAKLSNRIAKKEEKKQLKRKVKISVKKNVKS"
BASE COUNT         1527 a         1164 c         1462 g         1432 t
ORIGIN      
        1 aatcgaaagt ttggaaagtg gtgattacaa aggtttgcct gaaccaatat ttatcaaagg
       61 ttttattaaa acatacgccc gttttttggg tttggatatc gatcaaatta ccgtattgtt
      121 gaatgaggct tttccaagtc aagtgacggc aattgcttct acctccccag cagctgagag
      181 ccaaaatggt ttaaattttc aagatcagaa aaaaagcaaa agctttccaa aatggttgcc
      241 tttgctggtg gtcgctgcag cagtgatggg tgcagtgtat ttgtggcaat ccaaatccag
      301 tgagcaaaca cgtcaacaag aaaaatcgac cttggaagtt ggtgatgtgg cacaaccaca
      361 attggctacc gacaatgtac aagttgtacc tatgaatgct tctgctacct ctgccagcac
      421 tccagtcaat gcgagtgctc cgatggctgc ctcagctgcg gccagcggtg gtattcaaat
      481 caaggttcgt taccgttcta atttgattgt gaccgatgcc gatggcacac ctttgcacag
      541 tggcattgtg gcttcaaatt caagtcatga gtttaaaggc aaggcacctt atcaagtacg
      601 cattggttat gctacgggtt cagaaatcag cttcaatggt caaccaatag accttaaaaa
      661 tcatttcgtc gatggcaaaa cagttgcgtt aacggtgcca caaccatgag agagatcaaa
      721 cgtcgccaaa ctcaacaggt tcagataggt catgtttttg tcggttctga ccatcctgta
      781 ttggtacagt cgatgaccaa taccgatacc gcagatgccg aaggcaccgc cttacaagtg
      841 aaggctttgg ctgatgctgg ctctgaagtg gtgcgtatta cggtaaacag cccagaagcg
      901 gcctgtaaag tggcggaaat tcgccaacgc ttggatgaca tgggttgcaa cacaccattg
      961 gttggagact ttcacttcaa tggcgagcgt ttactgcgcg attatcaaga ctgtgccaag
     1021 gctttggcca aataccgcat caatcctggt aacgttggca aaggtgccaa aggcgatgaa
     1081 aaatttgcct acatgattga gcaagcagct ttgcatgata aagcggtgcg catcggtgtg
     1141 aactggggtt ctttagatca aagtttggcc aagaaaatga tggatgccaa tttggcattg
     1201 gccgaacctg ctcctgcaga tgcggtgatg aaagaggctt tgattacttc ggccttaaca
     1261 tcggcacaaa aagccattga tttgggtttg cctaaaaaca aaatcatctt gtcttgcaag
     1321 gtcagcaatg tccaagactt gattgaagtg tatggcgatt tggcacagcg ctgcgactat
     1381 cctttgcatt tgggcttgac cgaagcaggc atgggcagca aaggcattgt ggcttctagt
     1441 gcggccttgg cggtgttgtt gcaacgaggc ataggcgaca ccattcgcat ttctttgaca
     1501 cctgaacctg gtgcgccgcg cactcaagaa gtggtggtgg cacaagagtt gttgcaaacc
     1561 atgggtttgc gtaatttcac gcctttggta acggcttgtc caggttgtgg tcgcaccacc
     1621 agtactgtgt tccaagagtt ggcgcgtgat attcagcatt ttttgcgtga acaaatggcg
     1681 atttggcgct tgaaataccc tggcgtggaa agcctgaatg tggcggtgat gggctgtgtg
     1741 gtcaatggtc ctggtgaaag caagttggct gacattggca tcagtttgcc tggcacgggt
     1801 gaaacacccg tggcacctgt gtatgtggat ggtgagcgca aagtcacgct caagggcgac
     1861 cacatggcac aagaattcat tgccattgta gaagaatatg tagaaacgaa ttacggtgaa
     1921 aatggtgcca agcgccgtca aaatcgcgtg attccaattc aaactcgtta agatttaaga
     1981 agattatggg aaaaaaggtt caagctgtaa aaggcatgaa tgatttgctg ccagtggtac
     2041 aaaaagactg gcctatcaca tccgctttct ggttggcatt tgaacaaaga atcagcactt
     2101 gggcagagca atacgcttac caattcattc gcacacccat tattgagcca acaggcttgt
     2161 ttgtgcgctc cattggtgag gaaaccgatg tggtgggcaa agagatgtat acattcgatg
     2221 attatggtga atcactgagt ttgcgtcctg aaggtacggc atcttgtttg cgcgccgtgg
     2281 tagagcacaa tttgttgtat aacggcccga tgaagctgtg gtacatggga ccgatgttcc
     2341 gccatgaaaa accgcaaaaa ggccgttatc gtcaattcca tcaagttggt tgtgaggctt
     2401 taggctatag ccaacctgat gttgatgccg aaatgattgt catgactgcc gacttgtgga
     2461 agcaactggg catcttggat aaggtagaat tgcaaatcaa caccttgggc gtagcagaag
     2521 agcgtgctgc tcaccgtgcc gctttggtgg cttatttgca gcagcatgag gcgaaattgg
     2581 atgaagacag caagcgccgc atgcacacca atcctttgcg tgtgctcgat accaaaaatc
     2641 ccgatttaca agacatcgcc aataacgccc cgcgtttgat ggatttcttg ggtgaagaat
     2701 ctttggccca tcaccaagca ttcaaagctt tgctcgacag cgtgggcgtg gcttatgtgg
     2761 agaatccacg tttggtacgc ggcttggatt attacaacaa aaccgtgttt gagtgggtga
     2821 ccagtgagtt gggctctcag ggtacaattt gtggtggtgg ccgttacgat ggtttgatag
     2881 aagaattggg cggcaaagaa gcccctgcca ttggctttgc catgggcata gagcgcttgt
     2941 tgctgcttgt acagcaagca ggccaactag atgtggctgc aaatctggat gtgtttgtga
     3001 tgtaccaagg tgagggcaca atgcaacaag cattgacagt tgccactttg ctacgccaag
     3061 ccggcctgaa agtatcgcaa tattcaggta cacaaagcct gaaagcacaa atgaaaaaag
     3121 ccgatggcag tggcgctcgt ttcgccgtca tcatcggaga ggaagaattg gccaaggacc
     3181 aagtgagctt gaaagacttg cgtggcgaca aagggcagtt acaggccgct tgcagtgatt
     3241 tggtgcaaca attacaacaa tggatggtgt aatacatggc ttataatgtt gaagaacaac
     3301 aagacattga taacttcaaa cacttttgga gcaaaggtgg taagtggtta tttgttttat
     3361 tgctggccgc cggtttggcg tatttgggtt ttgtattgag caaatcttac gccaaggggc
     3421 aggatgaaga agcggctgca ttggcctacc aatttgtcga aaaaagcagc aaaaaagacg
     3481 cttctgcttt gaccgatttg aaaaccttgc aagaacaaca cggtaaatct tttggtgctg
     3541 ccgaagctac tttgatggca gcggcgcaag cgttcgatga aaagaaatac gaccaagctg
     3601 aaaaacacct gttgtgggta caaaagaaca acccagatgt gttcattcag gctttgtcta
     3661 atcagcgttt ggcggttatg aaattgcaac aacagcaata cgatgcggcc ttaaaattcg
     3721 ttgatgccac tgtgcctgcc gagttgaagc caatgatgct tgaaaccaaa ggcgatattt
     3781 tggctgccaa aggcgacaag gctcaggcca aaacggctta tgctgaagcc ttaaaaggct
     3841 tggatgaaaa gacatctccg aattacgaat tattgaaatt taaggcagaa caataaattc
     3901 gcattgagca cggctgccgc atagtctagg ctatgggcag ccgtcatgta tgtgaacctc
     3961 tgccttccct gtgaaggaat tgttatgaaa ccaactatcg tgttggtcgg ccgaccaaat
     4021 gtcggcaaat ctaccttgtt taatcgcctt acccgcagta aagatgcgtt ggtggccgat
     4081 ttgccaggtt taacccgtga ccgccattat ggccatggtc gcgtgggcga aaagccttat
     4141 ttggtagtag atacaggtgg ttttgagcct gtggtggaca gtggcatttt gtttgaaatg
     4201 gccaagcaaa ccttgcaagc ggtggatgaa gccgatgccg tggtgttctt ggtggatgcg
     4261 cgtgtgggcg tgaccccaca agacaagttg attgcacaaa aattgcgcat gagtggtcgg
     4321 cctgtattct tggctgtcaa caaaggcgag ggcacaaatc gcgcggtggt ggcagctgag
     4381 ttttatgaat tgggtttggc tgtagaaccc ttcgtgattt catcagcaca tggcgatggc
     4441 gtgtgggatt taatcagtgc cgtattggaa gaatttcctg atgctgttgc cgatgaagtg
     4501 gctaccgacc atcctgtatt tgcagtaatc ggtcgtccca atgtgggcaa atccactttg
     4561 gtgaatgcca tcttgggcga agagcgggtg attgcatttg accaagcagg cacgactcgg
     4621 gattccatac acattgattt tgagcgcgat ggcaaacctt ataccatcat tgataccgca
     4681 ggtgtgcgtc gccgtggcaa ggtggatgag gcgattgaga aattttcggt gattaaatcc
     4741 atgcaagcag tggaagctgc caacgtagcg gttttggtat tggatgccaa tcaagacatt
     4801 gccgaccaag atgctaccgt ggcaggtttt gctttggaag caggacgcgc attggtggtg
     4861 gcgattaaca agtgggatgt ggtcacgcct gaacgcaaag agcaaatcaa aaaagacatt
     4921 gcacgcaaac tgtatttctt ggattttgcc aaattccatt acatttctgc cttgaaacac
     4981 aaaggcattg atgatttgtt tggctcgata caccaagctt acaatgccgc catgattaag
     5041 atgccaacac cgaaaatcac tcgtgtgttg caatcggcct tagagcgtca acagccgccg
     5101 cgagctggat tgatacgacc aaaaatgcgt tatgcccacc aaggtggggt gaatccgccg
     5161 attattgtga tacatggcaa tgctttacac aatattaaag acagttacac tcgttatttg
     5221 atacagactt tccgtaaggc atttcatttg gaagggacac ctttgcgtgt tcagtacaag
     5281 tcttcagaga atccatttga agaaaaacgt gaacaaacct ctgcgcaacc tttgcgccgt
     5341 gcaaaattaa gtaatcgcat tgccaaaaaa gaggaaaaaa agcaactaaa acgcaaggtt
     5401 aaaatcagtg tgaagaagaa tgttaagtca tagtgtgtta ggatgttgca ttccatctgt
     5461 ttatggctta caaacagatg attatcacaa taaaaacatg gagtttaaag catgaacaat
     5521 aataaaggac aactcttaca agatcctttt ttgaacgcac tgcgcaaaga gcatgtgccg
     5581 gtgtc
//
LOCUS       DAOMVK010000094         5553 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_4029_length_5553_cov_3.897053, whole genome shotgun sequence.
ACCESSION   DAOMVK010000094 DAOMVK010000000
VERSION     DAOMVK010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 5553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5553
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4029_length_5553_cov_3.897053"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /gene="gcvT"
                     /locus_tag="PK856_04520"
     CDS             <1..875
                     /gene="gcvT"
                     /locus_tag="PK856_04520"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRL01671.1"
                     /translation="VDKLKFVGKALYSPMLNEQAGVIDDLIVYLSNEAETEYRIVSNA
                     ATRDKDLAQFTKTAAPFNVQIKERPELAMLAVQGPKAIEKLLQAKPQWAEQVNALTPF
                     VGVDLGGDWFVAKTGYTGEDGVEVILPAAEAVQFFQDLQAAGVHPCGLGSRDTLRMEA
                     GMNLYGHDMGEEINPLQAGMGWTVAWSDDRDFIGKAALLKAKETGVPEKQVGLVMETR
                     GVLREGMAVTVPGVGTGVITSGTFSPTLKLSIAIARVPKATTDTAQVDIRGNMTDVRV
                     IKLPFVRNGQKQFD"
     gene            947..1330
                     /gene="gcvH"
                     /locus_tag="PK856_04525"
     CDS             947..1330
                     /gene="gcvH"
                     /locus_tag="PK856_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRL01672.1"
                     /translation="MSNIPAELKYVASHEWLRLEADGSVTVGITDHAQELLGDIVFVE
                     LPEVGANLAADEQAGVVESVKAASDIYAPIAGEILEVNQELVDAPDTANTDPYGAAWF
                     FRIKPANAADLDGLMDAAAYEAETN"
     gene            1475..1939
                     /gene="yiaA"
                     /locus_tag="PK856_04530"
     CDS             1475..1939
                     /gene="yiaA"
                     /locus_tag="PK856_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HRL01673.1"
                     /translation="MALQEPSRAFVGASWTALAVGTITYLIGLWNAQVALSEKGYYLV
                     LILFGLFAAVSLQKAVRDRLEQIPVTEIYLGICWFGLMSSIVLLAVGLWNATMPGTEK
                     GFYGMSYLLSLFAAVAVQKNVRDTLQFRRENGIDTATHSGSFLQRRFGKDEE"
     gene            2030..2602
                     /locus_tag="PK856_04535"
     CDS             2030..2602
                     /locus_tag="PK856_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HRL01674.1"
                     /translation="MTYCQFAATLSADSTDPNKHYHDHVYGFSVEADNELFARLVLEI
                     NQAGLSWTTILKKWQGFYQAYAQFDIATVAAFNEEDMARLLADASIIRNRLKVQAAVY
                     NAQQIMGLQQEYGSFKTWLDHHHPLDKNAWVKLFKKHFKFVGGEIVNEFLMSTGYLAG
                     AHDADCPVLSQVLASKPLFAQTPLQDDTPC"
     gene            2596..3237
                     /locus_tag="PK856_04540"
     CDS             2596..3237
                     /locus_tag="PK856_04540"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HRL01675.1"
                     /translation="MLGIENYVGFIIAALVLNITPGSDTIYILSRTLAQGKRAGVASV
                     LGISSGVLVWGLLVSFGLATILKSMPSLMLLVKLLGAAYIVWIAIQMWRNSDLDAAAL
                     QAGNKHISIRKVYQQGIITNLLNPKVGLFFLAFLPQFVSVGTQSPLPFLLLTATFIVT
                     GTLWCLLLVGLASLISKQLQHKPHMASYISRGSSVILGSMGIHIGFSSLKEVL"
     gene            3234..4418
                     /locus_tag="PK856_04545"
     CDS             3234..4418
                     /locus_tag="PK856_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL01676.1"
                     /translation="MTETNPSGRLNLGLALAVFVIGVFAFLEVYAIQAILPILMHDFN
                     ASPAEIGFTVGATVLAVAIMSPFMGLLSDAIGRKKVIVASLLFLALPTAMLGFSSSLS
                     QMNVWRFLQGLAVPGITVVLIAYIGEEFSGKTMTRLMTLYVSGTVLGGFSGRFILGHL
                     TDYMTWQQAFWVMAAMMLIGVGLAYSQLPASKNFVPNAHLKPALQGLWSHLHNRYVLS
                     AMLLGSCVLFSLVGCFTFINLHLSAEPYHLSSSDLANIYMVYLIGMVITPLSTQIISR
                     FGAKKTVLMAVTVFMVGVIGTLSTPLWGIVLALAIMSTGVFITQAATISYIAKNVTQN
                     RSLASGLYYMGYYAGGTVGATVCGLAYARGAWGMTVVTLLAVQVFALLVASYIMVARK
                     AS"
     gene            4452..>5553
                     /locus_tag="PK856_04550"
     CDS             4452..>5553
                     /locus_tag="PK856_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HRL01677.1"
                     /translation="MNFNDLFHADEFVGRHIGLNADTAKEMLETVGASSIDDFLHQTV
                     PDSVRMPGELNLPDSITETDALKQLRGLADHITVNRSYIGLGYYPTRLPYVIQRNVLE
                     NPGWYTAYTPYQAEIAQGRLEALLNFQQVCIDLSGLELAGASLLDEATAAAEAMSLAK
                     RMSKVKSDRYFVDARVFPQTLDVMQTRAKYFGFELVVGDLAQAQDGEYFGAFLQYAGK
                     DGDVVDLTDVIAGLKAKGANVAVAADVMSLVLLKSPADMGADVALGNTQRFGVPMGFG
                     GPHAAYFAFTDAAKRSAPGRIIGVSVDAGGKRALRMALQTREQHIRREKANSNICTSQ
                     VLLANLAGMYAVYHGPEGVKRIATRIHAVATAF"
BASE COUNT         1287 a         1182 c         1495 g         1589 t
ORIGIN      
        1 atgtagacaa attgaaattc gttggcaagg ctttgtattc accgatgttg aatgaacaag
       61 cgggtgtgat tgacgatttg attgtttacc tgtcaaacga agccgaaacc gaataccgca
      121 tcgtgtctaa cgcggccacc cgcgacaaag acttggcaca atttaccaaa actgccgcgc
      181 cttttaatgt tcaaatcaaa gagcgtcctg aattggcgat gttggcagtg caaggaccta
      241 aagccatcga aaaactgtta caagccaaac ctcaatgggc agagcaagta aatgcgttga
      301 cgccatttgt gggtgtggat ttgggtggcg attggtttgt ggccaaaaca ggctataccg
      361 gtgaagacgg tgtggaagta attttgcctg cggctgaagc cgtgcaattc ttccaagact
      421 tacaggccgc tggtgtacat ccttgtggtt tgggttcgcg cgataccttg cgtatggaag
      481 cgggcatgaa tttgtatggc catgacatgg gtgaagaaat taatccattg caggccggca
      541 tgggctggac tgtggcttgg agcgatgacc gtgatttcat tggtaaggcc gctttgttga
      601 aagccaaaga aacaggcgtg ccagaaaaac aagtgggctt ggtgatggaa acccgtggcg
      661 tattgcgtga aggcatggct gtgaccgtgc caggcgtggg tacgggcgtg attacttctg
      721 gtaccttctc gccgacttta aaattgtcta tcgcgattgc tcgtgtccct aaagccacaa
      781 ccgacactgc tcaagtggac attcgtggca acatgaccga tgtgcgtgtg attaaattgc
      841 cttttgtgcg caatggccaa aagcaatttg actgattagt gtgtaggcgt tctgattgtt
      901 acaggacgcc ttttgtatat gaaatctaaa agtggagttt aacctcatga gcaatattcc
      961 agcagaatta aaatacgttg ccagccacga gtggttgcgt cttgaagccg atggttctgt
     1021 aaccgttggt attaccgatc acgcccaaga gttgttgggt gacatcgtgt tcgtagaatt
     1081 gccagaagtg ggtgccaact tggctgctga tgagcaagcg ggtgtggttg aatcagtgaa
     1141 agcggcttct gacatttacg cgcccatcgc tggtgaaatt ttagaagtga atcaagagtt
     1201 ggttgatgct cctgatactg ccaataccga tccatacggt gcggcttggt tcttccgcat
     1261 caaaccagct aacgcagctg atttggatgg tttgatggat gctgctgctt acgaagcaga
     1321 aaccaactaa gtcctgtttg ggatgtacca taagtagcaa gtgttgcact aaagtagatg
     1381 aatacttgct acttacactg aattaccacc gacttcccgc gatttattgc atgatggttt
     1441 tacctatttg gtaaatcagt aagggagtgg tgaaatggct ttacaagaac cgagtcgtgc
     1501 ttttgtgggt gcatcatgga cagctttggc tgtgggcacc atcacctatt tgattggctt
     1561 gtggaatgca caagtggcct tgagtgaaaa aggctattac ttagtattga tattgtttgg
     1621 tttgtttgcg gctgtttcct tgcaaaaagc agtgcgagat cgcttagaac aaataccggt
     1681 aaccgaaatt tatttgggta tttgttggtt tggtttgatg agttccatcg tgttgttggc
     1741 agtgggtttg tggaatgcca ccatgccagg cacggaaaaa gggttttatg gcatgagtta
     1801 tttgctcagc ctgtttgctg cggtagcggt gcaaaaaaat gtccgcgata ctttgcaatt
     1861 tcgacgtgaa aatggcatag acacagcaac tcattctgga tcgtttttgc aacggcgatt
     1921 tggtaaagat gaggaatgac ctgagaacct aaaccaaatt gaccccagtt tcctaccggt
     1981 gacagcatgg ctgtgaccgg ttttattttt ttgatggtta tgacgcgcaa tgacttattg
     2041 ccaatttgca gcgactttaa gcgctgacag caccgaccct aacaagcact accatgacca
     2101 tgtgtatggt ttctcggtag aggcagacaa tgaattgttt gcccgcttgg tattggaaat
     2161 caaccaagct gggttgagtt ggaccaccat actaaaaaaa tggcagggct tttaccaagc
     2221 ctatgcccag tttgatattg ccactgtcgc cgcatttaat gaagaagata tggcacgctt
     2281 actggctgat gccagcatta ttcgcaaccg cttgaaagta caggccgctg tttacaatgc
     2341 gcagcaaatt atgggcttgc aacaagaata tggttctttc aagacatggt tagaccacca
     2401 ccatcccttg gataaaaatg cgtgggtgaa actgtttaag aaacacttta agtttgttgg
     2461 tggcgaaatc gtcaatgagt ttctgatgag cacaggctat ttggctggcg cgcacgatgc
     2521 ggattgccca gtgttgtctc aggtgttggc gagcaagcct ttgtttgccc aaacaccgtt
     2581 acaggacgac accccatgct aggcatagaa aattatgtgg gctttatcat tgccgctttg
     2641 gtgttgaaca ttacgccagg ttcagacacg atttacattt tgagccgcac gttggcgcaa
     2701 gggaaacggg caggtgtggc ttcagtactg ggcatttcca gcggggtatt ggtttggggg
     2761 ctattggttt cttttggact ggccaccatc ttgaaaagca tgccatcact gatgttgttg
     2821 gtgaagttgc ttggggctgc gtacatcgtt tggattgcca ttcaaatgtg gcgcaacagt
     2881 gatttggatg cggcagcctt acaggccggc aacaaacaca tcagcatccg taaggtttat
     2941 cagcaaggca tcatcaccaa cttgttaaac cctaaagtcg gtttgttttt cttggcgttc
     3001 ttgccacagt ttgtcagcgt gggtacgcaa tcaccgcttc cttttttatt gctgacagcc
     3061 acttttattg tgacaggaac cttgtggtgt ttgctgctgg tgggcttggc cagcttaatc
     3121 agcaagcagt tacaacataa acctcatatg gcttcataca tcagccgcgg cagcagtgtc
     3181 atcttaggaa gcatgggcat acacatcggt ttcagtagcc tcaaagaggt gttatgaccg
     3241 aaaccaatcc gtctggccgt ttgaatttgg gtttggcact ggcggtgttt gtgatcggtg
     3301 tgtttgcttt tttagaggtg tatgccatac aggccatctt gccgattttg atgcatgatt
     3361 ttaatgccag tcctgcggaa ataggtttta cggttggcgc taccgtgttg gctgtggcca
     3421 ttatgtcacc ctttatgggc ttgttgtcag atgcaatagg acgcaagaaa gtcatcgtgg
     3481 cgtctttgtt gttcttggct ttacccactg cgatgttggg cttttccagc tcattaagcc
     3541 aaatgaatgt gtggcggttt ttacaaggtt tggccgtacc tggcatcacg gtagtgttga
     3601 ttgcttacat tggtgaagag ttttctggca aaaccatgac ccgtttgatg acgctgtatg
     3661 tgtcgggaac agtattgggt gggttttcag gccgcttcat cttagggcat ttgaccgatt
     3721 acatgacttg gcaacaggca ttttgggtga tggcggcgat gatgttgatt ggtgtgggtt
     3781 tggcttacag ccaattgccc gcctcaaaga attttgtgcc caatgcacat ttgaaacccg
     3841 cgctgcaagg cttgtggtca cacttgcaca accgttatgt gctcagtgcg atgttattgg
     3901 gttcgtgtgt gttgttttca ttggtggggt gtttcacctt catcaatttg catttatcgg
     3961 ccgagccgta ccacttaagc agttcggact tggccaatat ttacatggtg tacttgatag
     4021 gcatggtgat tacgcccttg tctacgcaaa tcattagccg cttcggtgca aagaaaacgg
     4081 tgttgatggc ggtgacagta tttatggtgg gcgtgatagg cactttgtct acgccgcttt
     4141 ggggcatcgt gttggccttg gccatcatga gcacaggcgt gtttattacg caagcggcta
     4201 ccatcagtta catcgccaaa aacgtgactc aaaaccgttc tttggcatcg ggtttgtatt
     4261 acatgggtta ttacgctggt ggcacagtag gtgccacggt gtgtggtttg gcttatgcgc
     4321 gcggggcttg gggcatgaca gtggtgacct tgctcgcggt tcaagtgttt gctttattgg
     4381 tggcgtctta cattatggtg gctcgtaagg cgtcctgaaa aacgatggtt tttgtaacga
     4441 aggattcagt tatgaatttt aatgatttgt tccacgccga cgaatttgtc ggtcgccata
     4501 tcggtttaaa cgctgacacg gcaaaagaaa tgcttgagac cgtgggtgct tctagcatcg
     4561 atgatttctt gcatcaaact gtgcctgatt cagtacgcat gccaggcgaa ttgaatttgc
     4621 cagacagcat cactgaaacc gatgccttga aacaattgcg cggcttggct gaccacatca
     4681 ccgtgaaccg cagctacatc ggcttgggct attacccaac ccgtttgcca tacgtgattc
     4741 aacgcaatgt gttggaaaat ccaggttggt acacggctta caccccttac caagctgaaa
     4801 ttgcccaagg ccgtttggaa gctttgttga atttccaaca agtgtgtatc gacttgtctg
     4861 gtttggaatt ggctggcgcg tcattgctcg acgaagccac tgctgctgct gaagccatgt
     4921 ctttggccaa acgcatgagc aaagtcaaat ctgaccgtta ttttgtggac gctcgtgtgt
     4981 tcccacaaac tttggacgtg atgcaaaccc gtgccaaata cttcggtttt gaattggtgg
     5041 tgggtgattt ggctcaagcc caagatggcg aatacttcgg tgctttcttg caatatgcgg
     5101 gcaaagacgg cgacgtggtt gatttgaccg acgtgattgc cggcctgaaa gccaaaggcg
     5161 caaacgtggc tgtggctgcc gatgtgatga gcttggtatt gttgaaatct cctgctgaca
     5221 tgggtgctga tgtcgctttg ggtaacaccc aacgctttgg tgtgcctatg ggctttggtg
     5281 gccctcacgc cgcgtatttt gcctttaccg atgctgccaa acgctcggct cctggccgta
     5341 tcattggtgt gtctgtggat gctggcggca aacgtgcttt gcgcatggct ttgcaaaccc
     5401 gtgaacaaca cattcgccgt gaaaaagcca actctaatat ctgtacgtca caagtgttgt
     5461 tggccaactt ggcaggcatg tacgcggttt atcacgggcc agaaggcgtg aaacgcattg
     5521 ccacccgcat ccatgcagta gcaactgcat ttg
//
LOCUS       DAOMVK010000095         5551 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_4031_length_5551_cov_3.863719, whole genome shotgun sequence.
ACCESSION   DAOMVK010000095 DAOMVK010000000
VERSION     DAOMVK010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 5551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5551
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4031_length_5551_cov_3.863719"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1328
                     /gene="cysG"
                     /locus_tag="PK856_04555"
     CDS             <1..1328
                     /gene="cysG"
                     /locus_tag="PK856_04555"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HRL01678.1"
                     /translation="QVAERKVDSLLQSGALVRIVAQSLSPLLQQWLDEGRISHWGEQF
                     HANMVDEVFLVIAATDDATLNQQVFQAAEAAHTLCNVVDDLLHCNFIVPAVVDRGAVQ
                     VAISSGGTAPVLARIWRQKIESLLPHHLHDAATIAGRWRSKVKQHIHSMADRRRFWEQ
                     VFHSRFDTLTAQGDLQAAERELESQLQGHHSQIGEVVLVGAGPGDAGLLTLKGLQALQ
                     DADVVLYDALVSDDILQLIRKDAERISVGKRAGNHSVMQEKTNELLVEYAQQGKRVVR
                     LKGGDPFVFGRGGEELQVLQAASIPYRVVPGITAALGATAYAGIPLTHRSFAQTATFI
                     TGHCQADGSDVDWQSLALSRHTLVVYMGTINAATIATQLQHFGRSSDTPVAVISNGTR
                     QNQSVVTGVLSDLATLAHNAPSPALLIIGEVVSLQAELNWFGEHADACA"
     gene            complement(1604..3040)
                     /locus_tag="PK856_04560"
     CDS             complement(1604..3040)
                     /locus_tag="PK856_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRL01679.1"
                     /translation="MSSSGNQLEGGLKSRHLTMLSIAGVIGGALFVGSGKVIANAGPA
                     AILAYLGGGILVVLVMRMLGEMAVAQPDTGSFSTYADRAIGRWAGFTIGWLYWSFWSL
                     LMGWEAYVAGAILNSWIPAIPIWGYMLIVTLALMAVNFMHVKNYGEFEFWFALIKVVA
                     IVAFIIIGALAIVHIWPFGDVSGWEHLTSQGFMPNGFDAVVVALLGAMFAFIGAEIVT
                     VAAAEAKNPSKEIIRTTRSVVWRISLFYIGSIFIVVCLIPYTEPGLKDSTLGTYAVTM
                     QHLGIPYAKQIVNFVVLTSVCSCFNSALYTSSRMLFSLSRRGDAPRAFQILGKNTKAP
                     YVGVLASSILALITIVLTATDKMNVFDVLMAATGAIALLVYLCIAVSQLNMRRKLQAS
                     GAEIQLKMWLFPWLTYAVIFVIVASLITMVVEGTFFTEVVYTSIFGGIVVVLGMMAQK
                     YNWGGEQRAQAEKERLEIQAQLNQNKEA"
     gene            complement(3466..3798)
                     /locus_tag="PK856_04565"
     CDS             complement(3466..3798)
                     /locus_tag="PK856_04565"
                     /inference="COORDINATES: protein motif:HMM:NF017273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HRL01680.1"
                     /translation="MSNTVFLIALACMSAITFVLRAAPTLLPRKWLESPWLNALNFAL
                     PLSVMMVLILAALPLQSAVVEQNWRELVAQVLALLLVLMVYARWRNVLVAMVTGVAVL
                     NLLLYLFN"
     gene            complement(3795..4478)
                     /locus_tag="PK856_04570"
     CDS             complement(3795..4478)
                     /locus_tag="PK856_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HRL01681.1"
                     /translation="MSQQSAWKEALRLSMPVGMGYVPAAIAFGVLMSAAGLPWWWALL
                     LSLFLYSGAAQYAAIPMLAAGAGVASLSVNTLVINLRHVFYALPLLQHLPKHHVKRWY
                     ALFALTDESFSLLTTMPAETARKLFVKIVWLNQMYWVIGTLIGLGIGAGLNELIPHLD
                     FALTCLFVVLAYEQYQAKRVWWPCVLAVVAFVLASVVTVDYLLLLAVALCALGILLWQ
                     WRQAKRSVA"
     gene            complement(4486..4695)
                     /locus_tag="PK856_04575"
     CDS             complement(4486..4695)
                     /locus_tag="PK856_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003783673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HRL01682.1"
                     /translation="MQDFDTRITELEIRLALQDDTIDVLNKQINELYEQLSLQQQQLQ
                     LLYRKFSEAADNQISTHGNEPPPHY"
     gene            complement(4799..5221)
                     /locus_tag="PK856_04580"
     CDS             complement(4799..5221)
                     /locus_tag="PK856_04580"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003741652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HRL01683.1"
                     /translation="MSTMQVEVVSNEATIYSGEASFVVVPTESGELGIYPQHVPILSK
                     VRPGVLRLQVPGQAEEVTVAIAGGLLEVQPSKITVLADVAVRSQELDAERAKSALEAA
                     EKQLKAADSDKARATAEAALAAAIAQLKTLDHLRNRRH"
     gene            complement(5232..>5551)
                     /locus_tag="PK856_04585"
     CDS             complement(5232..>5551)
                     /locus_tag="PK856_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRL01684.1"
                     /translation="VARGVQATLQKYKELRDIIAILGMDELSEEDKLTVMRARKIQRF
                     LSQPFHVAEVFTGSPGKYVPLRDTIKGFKAILNGEYDHLPEQAFYMVGGIEEAAAKAE
                     TLN"
BASE COUNT         1561 a         1509 c         1203 g         1278 t
ORIGIN      
        1 gacaagtggc ggaacgcaaa gtcgacagtt tactacagag tggtgcgctc gtgcgcatcg
       61 tggcacaaag cttgagccct ttgctacaac agtggctgga tgagggccgc atcagccact
      121 ggggcgaaca attccatgca aacatggtgg acgaagtctt tttggtgatt gccgccactg
      181 acgatgccac cttaaaccaa caggtttttc aagccgctga ggcagcacac accttgtgca
      241 atgtggtgga cgatttgctc cattgcaatt tcatcgtgcc tgccgtggta gaccgcggtg
      301 cggtgcaagt ggcgatttcc agcggtggca cggcacctgt gttggcacgg atttggcgcc
      361 aaaaaattga gtctttatta ccacatcatt tgcacgatgc cgccaccatc gcaggacgct
      421 ggcgcagcaa agtcaaacaa cacatccaca gcatggccga cagacgccgc ttttgggaac
      481 aggtgttcca cagccgcttt gatactttga ctgcacaagg cgatttacag gccgccgaac
      541 gcgagctaga aagccaatta caaggccatc acagccaaat aggcgaagtg gtattggtag
      601 gcgcaggccc aggcgatgcg ggcttgctca ccctcaaagg cttgcaagcc ttacaagacg
      661 ccgatgtggt gttgtacgat gccttggtga gcgatgacat tttgcaactc atacgcaaag
      721 atgccgaacg catcagcgtg ggcaaacgcg cgggcaatca cagcgtgatg caagaaaaaa
      781 ccaatgaatt gttggtggag tacgcacagc aaggcaaacg tgtggtgcgc ctcaaaggcg
      841 gcgacccatt tgtgtttggc cgcggcggcg aagaattgca agtgttacag gccgcttcta
      901 ttccctatcg cgtcgtgcct ggcattaccg ccgccttggg cgcaaccgct tacgcgggca
      961 tacccttaac ccaccgcagc tttgcccaaa cggctacttt catcacaggc cactgtcaag
     1021 ccgatggcag cgatgtcgat tggcaaagtc tggccttgag ccgccatacc ttagtggtgt
     1081 acatgggcac catcaatgcc gccaccatcg ccacccaatt acagcatttc ggtcgcagca
     1141 gcgacacacc tgtcgccgtc atcagcaatg gcacgcgcca aaaccaaagc gtggtcacag
     1201 gggtgttaag cgatttggca actcttgcac acaatgcgcc ctcacctgct ttgctcatca
     1261 tcggtgaagt ggtgtcttta caggccgagt taaactggtt tggcgaacat gcagacgctt
     1321 gcgcttaacc aagcaaagac ataaaacaag gtggccatgc caccttgttt tattttaagc
     1381 gtacaaatca gtatccctat caccagaggc aaggcgattt attctcagct catccgcgtt
     1441 tccacctccc agccatacaa ctcttactct ctcaatggta gtctatggtg ttttcaatga
     1501 tgcactgatt tacaggccgc ataggcagta cacttacgtt tggcttaatc ctgaacacaa
     1561 aaaagcccgc aatatgtgcg ggcttttgca aacactcaaa acattatgct tctttgtttt
     1621 ggttcaactg agcttggatt tccaaacgct ctttctcagc ctgagcgcgt tgctcaccac
     1681 cccagttgta tttctgcgcc atcatgccca ataccaccac gatgccaccg aaaatactgg
     1741 tgtaaaccac ttcagtgaag aaggtgcctt ctaccaccat ggtgatcaaa gaggccacaa
     1801 tcacaaaaat caccgcataa gtcagccaag ggaataacca cattttcagt tgaatctcag
     1861 cacctgaagc ttgcaatttg cgacgcatgt tcaattgcga taccgcaata cacaagtaca
     1921 ccaacaaggc aattgcaccc gtagccgcca tcaacacgtc aaacacattc attttgtcgg
     1981 ttgcggtcaa cacaatggta atcaaagcca aaatactgga agccaacaca cccacatacg
     2041 gtgctttggt gtttttaccc aaaatttgga acgcgcgcgg tgcatcacca cggcgagaca
     2101 aagagaacaa catgcggcta gaggtgtaca aggctgagtt aaagcagctg cacacagagg
     2161 tcaacaccac aaagttcacg atttgcttgg cataaggaat gcccaaatgc tgcatggtca
     2221 ccgcgtaagt gcccaaggtt gagtctttca aacctggctc ggtgtaagga atcaagcaca
     2281 ccacgatgaa aatcgagcca atgtagaaca aggaaatgcg ccataccacc gaacgcgtgg
     2341 tgcggatgat ttctttgctt gggtttttgg cttctgctgc tgcaaccgtc acaatctctg
     2401 cgccaataaa ggcaaacatc gcaccgagca aggcgaccac cacggcatca aaaccgttag
     2461 gcataaaacc ttgactggtc aaatgctccc aaccgctgac atcgccaaac ggccaaatgt
     2521 gcacaattgc caaggcacca atgatgatga aggccacaat cgccaccact ttaatcaagg
     2581 caaaccagaa ttcaaattcg ccataattct tcacatgcat gaagttcacc gccatcaagg
     2641 ccaaggtgac aatcagcata tagccccaaa tgggaatggc aggtatccat gaattcaaaa
     2701 tcgcccctgc cacataggct tcccaaccca tcaacaaaga ccaaaaactc caatacaacc
     2761 aaccaatggt aaaacctgcc caacggccaa tggcgcggtc ggcataggtt gaaaacgaac
     2821 ctgtatcagg ctgcgctacc gccatctctc ccagcatgcg catcaccaat accaccaaga
     2881 tgccaccacc caaataagcc aaaatggccg cagggcctgc attggcgatg actttaccag
     2941 agccaacaaa caaggcccca ccaatcacgc ccgctatcga caacatcgtc agatgccgtg
     3001 atttcaagcc gccttctaat tgatttccag aactgctcat accgatactc ctcaccttgt
     3061 tacttttgct tgtccttcat acagatgtaa tcggacaaaa cttttttatt aagattttta
     3121 ttcttgcatt gcagcacgaa tatacctttc tataaaaaat gacacaatat caacatgata
     3181 gttatttatt cttgttaata tattttgtat tgcagaaatg agttttgcct agtgaaacac
     3241 cgttgatttt ataagcataa ttttgattaa gtatcactgt tcattattaa caatgaactg
     3301 acatattttt ttcatgtcga ttttaattac ataaattttg cgatttatgc tgctctaatc
     3361 tgggcattta cttattcata atactgtgca taaaaccctt gattaaaata ccaaaaataa
     3421 agcggcctgt aggtttacag gccgcttgtt tcatccatat acacattaat tgaatagata
     3481 aagcaataag tttaaaaccg ccacacccgt gaccatcgcc accaaaacat tgcgccaacg
     3541 ggcatacacc atcaacacca ataacaaggc caaaacctgt gcgaccaact cgcgccaatt
     3601 ctgctctaca accgcacttt gcaaaggcag tgctgccaaa atcaacacca tcatcacact
     3661 taacggcaag gcaaaattca gggcattaag ccaaggcgat tccagccatt tacgcggcaa
     3721 taaagtcggt gcagcgcgca aaacaaaggt aatggcactc atgcacgcca aggcaatgag
     3781 aaaaaccgta ttgctcatgc cacgctcctt ttcgcttgtc gccattgcca caacaaaatg
     3841 cccaatgcac acaaagccac agccaacagc aacaaataat ccaccgtcac cacgctcgcc
     3901 aacacaaatg ccaccacagc caacacgcaa ggccaccaca cccgtttggc ttggtattgc
     3961 tcataagcca acaccacaaa caagcaggtg agcgcaaaat ccaaatgcgg tatcaattca
     4021 ttcaagccag ctcctatgcc caagccaata agcgtaccga tgacccaata catttgattg
     4081 agccagacaa ttttcacgaa cagtttgcgc gcggtttcag caggcatggt ggtcaacaag
     4141 gaaaagctct catcggtgag cgcaaacaag gcataccaac gttttacgtg gtgtttaggc
     4201 aagtgttgca gcaaaggcaa ggcataaaac acatggcgta aattgatgac caaggtattc
     4261 acactcaatg aggccacgcc cgcccctgcc gccagcattg gtatcgccgc atattgcgcg
     4321 gcacccgaat acaaaaacaa actgagcaac agcgcccacc accatggcaa gcctgccgcg
     4381 ctcatcaaaa caccaaacgc aatggctgcg ggcacatagc ccatgcccac aggcatgctc
     4441 aagcgcagcg cttctttcca agcagattgt tgtgacatac gacactcaat aatggggagg
     4501 cggctcgttg ccgtgggtag atatttggtt gtcagcggct tcactgaatt tgcgatacaa
     4561 caattgcagc tgttgctgtt gcaaggacag ctgttcgtac agctcgttga tttgcttgtt
     4621 cagcacatca atggtgtcgt cttgcaaagc cagacgaatt tccaattcag taatgcgggt
     4681 atcaaagtct tgcatgggag tctttcatgg ctgtgcacaa caaagccatc attttacctt
     4741 tacaggccgc ttgataacaa aaaaccgccc gaaggcggtt tttatcagac agcttggatt
     4801 aatgacggcg gttgcgcaaa tggtccaagg ttttcaactg tgcaatggca gcagccaatg
     4861 cggcctctgc agtggcacgt gctttatcgc tgtcggctgc tttcaattgt ttctcagcag
     4921 cttccaatgc agatttcgca cgttcagcat ccaactcttg gctgcgaacc gcaacgtctg
     4981 ccaatacggt gattttagaa ggctgaacct ccaacaaacc accggcaatg gcaacagtca
     5041 cttcttccgc ttgaccaggc acttgcaaac gcaatacgcc tggacgaacc ttactcaaaa
     5101 tcggcacgtg ttgtggataa atacccagct caccgctttc ggtaggcacg acaacaaagc
     5161 tggcctcacc cgaataaatg gtggcttcgt tactcaccac ttcaacttgc atggtactca
     5221 tgcttgcctc cttagtttaa ggtttcagct ttagctgcag cctcttcaat gccaccaacc
     5281 atgtagaacg cttgttctgg caagtggtcg tattcaccat tcaaaatggc tttaaagcct
     5341 ttgatggtgt cacgcaatgg aacgtatttg cctggtgaac cagtaaatac ttcggccacg
     5401 tggaatggtt gagacaagaa acgttggatc ttacgagcac gcatcacagt caatttgtct
     5461 tcttcagaca attcgtccat acccaaaatc gcgatgatgt cacgcaattc tttgtatttt
     5521 tgcaatgttg cttgtacgcc acgcgctact g
//
LOCUS       DAOMVK010000096         5537 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_4049_length_5537_cov_3.165998, whole genome shotgun sequence.
ACCESSION   DAOMVK010000096 DAOMVK010000000
VERSION     DAOMVK010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5537
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4049_length_5537_cov_3.165998"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PK856_04590"
     CDS             complement(<1..368)
                     /locus_tag="PK856_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRL01685.1"
                     /translation="MCGVFGLVSHEPVNQLLYDGLQMLQHRGQDAAGIVTASGQSFHM
                     HKGSGMVRDVFRTRNMRDLIGNAGIAHVRYPTAGNSKSAAEAQPFYVNSPFGIVLAHN
                     GNLTNTDELFENVCYRDLRH"
     gene            complement(380..880)
                     /locus_tag="PK856_04595"
     CDS             complement(380..880)
                     /locus_tag="PK856_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HRL01686.1"
                     /translation="MAVFDLLVLVVLGLSVVVSMVRGVVAEIASLITWLVALVAARVL
                     SPVTAQMVFTRVDSAVFANVLAFIVVFITTYFLLSCAQKLLTSAIQAIGLGGINKFFG
                     ALFGLIKGTFLITVVVLLCALTDLPRTEDWKNAQLAPTFEQLALLTVPYLPAFVAGRI
                     HYSPSE"
     gene            complement(885..2030)
                     /locus_tag="PK856_04600"
     CDS             complement(885..2030)
                     /locus_tag="PK856_04600"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRL01687.1"
                     /translation="MVQKKTENEYDQLKRQNRRRLVGAVVMVVAAGGILASTGSFGSK
                     TAETEEGKLALVAKDGKQVQGNTTVLPKVTVHNQQDNTVQNVETEADKQQAAEKIRQE
                     QTAAEEARKKQPADESQQRQGQVDPVANQETTVQQLEKKRQQEAEAARLQQLQEDKQR
                     KQELAFLAKEQAEKEKADKLAAEKAKLKVEQTKLEKDKADKAEAAQKLAEQRKAEAVL
                     AEEKKAADLKQRKAELAKQEQERKLAESKKANEAKQLAEAKKAAEAKKAAEAKKIADA
                     KQLADAKKTADAKKAADAAKAKAALANKAPVAANTKSVVQVAAFKSDDKAQQIHKEVQ
                     ALGLPSSVARVGNMARVRVGPYRNPADAEKAAAKLKLHGFNALIMQY"
     gene            complement(2051..2410)
                     /locus_tag="PK856_04605"
     CDS             complement(2051..2410)
                     /locus_tag="PK856_04605"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRL01688.1"
                     /translation="MNHRHIRTVLSVAMLGMLTGMAQAAVVNNTDQNQAKVVKEPVKA
                     KKYVVQLGAFVDEFQALHRRQQWTEAGIKTYVLPASGDSGMLRIRSGPFDSKQEAQAM
                     VLQLKLVGVENAIILLE"
     gene            complement(2494..3786)
                     /gene="folC"
                     /locus_tag="PK856_04610"
     CDS             complement(2494..3786)
                     /gene="folC"
                     /locus_tag="PK856_04610"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HRL01689.1"
                     /translation="MSLSLQQWLTQLEQGMVGGVIDLGLERTRAVKERLALSPKCPVV
                     IVGGTNGKGSVCAFLTQMYVQAGYKVGTLTSPHLQHYNERIAINGVPSSDEQIIISFE
                     RIQAACKDDITLTYFEYNVLAAVDVFDRVRVDVMVLEVGMGGRLDAVNVFDADVTVVT
                     SVDVDHQAFLGDNIEQIAFEKAGIFRAGKPAVVAQVPLPESMRAHAEDLGTPILLLQR
                     DFAYVKQEQQQWSFRFHPQQTDLDLAEHKRHALPIPALRGAYQMSNAACALAVLECLN
                     AQLPVDMGAIKRGLLLVSNPGRFQVLPGRPLVVWDVGHNPHAARALRNGLLALPYAQK
                     RMAVFSMLADKDIASVLEILKDQFDEWLIAPLDMPRGMNTEDLQAALNQAGIDAVVSF
                     PNIAKAYQSALSQATENDRIVAFGSFHTVAEVMGIPRY"
     gene            complement(3783..4175)
                     /locus_tag="PK856_04615"
     CDS             complement(3783..4175)
                     /locus_tag="PK856_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01690.1"
                     /translation="MYLIVILSLHLLGAALMVLMLTSPLLWVCLGMLLLSGLWAGRKW
                     WHSATYFLDIKADGKVLLGLQADTVDEVSLQDGGLLTAYLLLMRWQNKAGKCHTTVVW
                     RDSVSEKTFKLLYVWLRWQPQQHEVKSK"
     gene            complement(4214..4564)
                     /locus_tag="PK856_04620"
     CDS             complement(4214..4564)
                     /locus_tag="PK856_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01691.1"
                     /translation="MYFVDRSAVVLKPTQVFLDWLKSVDEDMPELSLAQIRSNCTVLL
                     IPEVDEPEEAVAYLDERFEEVFRNELSGWEVPQNLWPKNMDLVTFWQFFEVEIHDLVL
                     DSVDDELMVQPISS"
     gene            4727..5537
                     /locus_tag="PK856_04625"
                     /pseudo
     CDS             4727..5537
                     /locus_tag="PK856_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003786090.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
BASE COUNT         1433 a         1489 c         1139 g         1476 t
ORIGIN      
        1 atgtggcgca aatcgcggta acacacgttt tcaaacaact catcggtatt ggtcaggttg
       61 ccattgtggg ccagcacaat accaaaaggt gaattcacat aaaaaggctg tgcttcagcc
      121 gcacttttag aattaccagc ggttggataa cgcacatggg caatgccagc attgccaatc
      181 aaatcacgca tattgcgggt tctgaacaca tcacgcacca taccgctgcc tttgtgcatg
      241 tgaaaagatt gcccactggc agtgactatc cctgctgcat cttgaccgcg atgttgcaac
      301 atttgcaaac cgtcataaag caattgatta acaggctcat gagacaccaa accgaaaaca
      361 ccacacatgg aaaaattcct tactcgctag gagaataatg aatacgtcct gccacaaagg
      421 caggcagata gggaactgtc aataacgcca attgttcaaa tgtgggggcc aattgtgcat
      481 ttttccagtc ctcagttcga ggtaaatcgg tcaatgcaca cagcaatacc accacagtaa
      541 tcaaaaaagt ccctttgatt aacccaaaca atgcgccaaa gaatttattg atgccaccta
      601 agcctatggc ttgtatggca gaagtcagta atttttgagc acaagaaagc aaaaaatatg
      661 tggtgatgaa caccacaata aacgccaaaa cattggcaaa taccgctgaa tccacacgtg
      721 taaacaccat ttgtgcagtg actggcgaca acacccgtgc tgccaccaaa gccaccaacc
      781 aggtgatcaa ggaggcaatt tcagccacca ctccccgtac catggacacg acgacactca
      841 gccccaaaac caccaaaacc aataagtcaa atacggccat tttttcaata ctgcataatc
      901 aaagcattaa atccatgcag cttgagtttg gctgcagctt tttcggcatc ggctggattg
      961 cgatatggcc ccactctgac acgtgccata ttgcccactc gcgccactga agaaggcaag
     1021 cccaaggctt gtacttcttt gtgtatttgt tgcgctttat catcgctctt aaaagcggca
     1081 acctgcacca cagacttggt attggcagca actggggctt tatttgccaa cgccgctttg
     1141 gctttagcag catcggctgc tttttttgcg tccgcagtct ttttggcatc tgccagctgc
     1201 ttggcgtctg ctattttttt cgcttcagca gcttttttcg cctcagctgc tttcttggct
     1261 tcggccaatt gtttggcctc gttcgctttt ttgctttcag ccagttttcg ttcttgttct
     1321 tgcttagcca attcagcctt gcgttgtttt aaatcagccg ccttcttctc ttctgccaat
     1381 acagcctctg ctttgcgttg ctcagcaagt ttttgcgcag cctcagcctt atcggccttg
     1441 tctttttcta gcttggtctg ttcaaccttt aatttggctt tttcagcggc tagcttatct
     1501 gccttttctt tttcagcttg ttccttggcc aaaaatgcca actcttgttt gcgctgcttg
     1561 tcttcttgca attgctgtaa tcgagccgct tctgcttctt gctggcgttt tttctctagc
     1621 tgttgcacag tagtttcttg gttggcgaca ggatccactt ggccttgacg ttgttgtgac
     1681 tcgtcagccg gctgcttttt tcgagcttcc tccgctgcgg tttgctcctg gcgaattttt
     1741 tctgcagcct gttgcttgtc agcttcggtt tctacgtttt gtacggtgtt gtcttgttga
     1801 ttgtgaaccg tgactttagg taacacggtg gtattgcctt gaacctgttt gccatctttg
     1861 gcgaccaaag ccagcttacc ctcttcggtt tcagctgttt ttgaaccaaa actgcctgta
     1921 ctggccaaaa taccacccgc agccaccacc atcactaccg cacccaccaa acggcgacgg
     1981 ttttgacgct tcaactgatc atattcgttc tctgtcttct tttgcaccat aacaatccta
     2041 aattttcaag tcactcaagc aaaataatgg cattttccac gcccaccaat ttcaactgta
     2101 acaccatggc ttgcgcctct tgctttgagt caaacgggcc actgcgtatg cgcagcattc
     2161 cactgtcacc actggcaggc aatacatagg ttttaatgcc cgcttcggtc cattgttggc
     2221 ggcgatgcag tgcttgaaac tcatccacaa acgcccccag ttgaaccaca tatttcttag
     2281 ccttaacagg ctctttaacc actttagcct gattttgatc ggtattattg accactgccg
     2341 cctgagccat cccagtcagc atacccaaca tggccacgct caggactgtt cgaatgtgtc
     2401 gatgattcac gtcctatccc cttaaaaatg atgtcacact tattcgatat tatgatttga
     2461 attgaaagtg tttttgacta gttttggctc taatcaataa cgtggtatgc ccatgacttc
     2521 cgcaacggtg tggaaagagc caaacgcgac gattctatca ttttctgttg cctgtgataa
     2581 agcgctttgg taagccttgg caatgttagg aaaagaaacc acagcatcaa tgccagcttg
     2641 attcaaagcg gcctgtaaat cctctgtatt catgccacgg ggcatgtcta atggggcaat
     2701 cagccattca tcaaactggt cttttaatat ctccaacaca ctggcgatgt ctttgtctgc
     2761 caacatggaa aacacagcca tgcgcttttg tgcataaggc aaagccaaca aaccattgcg
     2821 taaagcccgc gccgcatgcg gattgtgccc cacatcccac accaccaaag gacgacctgg
     2881 cagcacttga aagcgaccag gattgctcac caataacaaa ccccgcttaa tcgcacccat
     2941 gtctacaggc agttgtgcgt tcaaacactc taataccgcc aaggcacaag cggcattgct
     3001 catttgatat gcaccgcgca gcgcaggaat gggcaaggca tggcgcttgt gctctgccaa
     3061 atccaaatct gtttgctgcg gatgaaaacg gaatgaccac tgctgttgct cttgcttcac
     3121 ataggcaaag tcgcgttgca acaacaaaat tggcgtaccc aaatcctcag catgagcacg
     3181 catggattca ggcaaaggca cctgtgccac caccgcaggt ttgcccgcgc ggaaaatacc
     3241 ggctttttca aatgcaattt gttcgatgtt atcgcccaaa aaggcttgat ggtctacgtc
     3301 gacactggtg accacggtca catcggcatc aaacacattc accgcatcca aacgcccacc
     3361 catgcccact tccaatacca tcacatccac acggacacgg tcaaacacat ccacagccgc
     3421 caacacattg tattcaaaat aggttaaggt tatgtcgtct ttacaggccg cttgtatgcg
     3481 ctcaaaactg atgatgattt gctcatcgct gctgggcaca ccattgatgg caatgcgctc
     3541 attgtaatgt tgcaaatgcg ggctggttaa agtgcccact ttgtagccgg cctgtacgta
     3601 catttgggtt aaaaaagcac acaccgaacc cttaccattg gtgccgccca caatgaccac
     3661 gggacacttg ggcgacaaag ccaaacgctc tttcactgcg cgagtacgct ccaaacccaa
     3721 atcaatcaca ccaccaacca taccctgctc aagctgtgtc aaccactgct gcaaactcaa
     3781 actcatttgc tttttacctc gtgttgctgt ggctgccagc gcaaccacac atacaataat
     3841 ttgaatgtct tctcagagac gctgtcacgc cataccacag tagtgtgaca tttacctgct
     3901 ttgttttgcc aacgcataag caataaatat gcagtcaaca gcccaccatc ttgcaaactt
     3961 acttcatcca cagtatcggc ctgtaaaccc agcaacactt tgccatcggc tttgatgtcc
     4021 aaaaaatagg tggcactgtg ccaccatttt cgccctgccc ataaaccaga caacagtaac
     4081 atacccaaac acacccaaag caagggtgag gtaagcatca aaaccatcaa cgcagcaccc
     4141 aataagtgta gactcagtat cacaatcaga tacatgcgtg accttattgg ctttacgctg
     4201 atagcataca tgattagctg ctgatgggtt ggaccatcaa ttcatcatcg acagaatcca
     4261 gcaccaagtc gtgaatttcc acctcaaaaa actgccaaaa agtcaccaaa tccatgtttt
     4321 taggccataa attctgtggt acttcccaac cagacaattc gttgcgaaac acctcttcaa
     4381 aacgctcgtc caaatacgcc acagcctctt caggctcatc cacttcggga atcaacaata
     4441 ctgtgcaatt actgcgtatt tgcgccaagc tcaattctgg catgtcttca tccacgcttt
     4501 tgagccaatc caaaaacacc tgtgtgggct tcaacaccac cgcgctgcga tctacgaaat
     4561 acatgatgtg catccttcaa aaatattgca ccattgtact ctgttgctcc gcctctggca
     4621 cgcttcaggc cgcttaagaa atgattttag cactgcttaa gtttgtggct tgcggtaaaa
     4681 tagcgttctt atttatttgt cagcactttt tattagcaaa gccaccatga atattttatc
     4741 tgtagatcac gcacagtttg ccgttggcca tgtcgcctta cttgatgatg ccgaatttca
     4801 cttagctcaa ggcgagcgca taggtttgat tggccgcaat ggcgcaggta aatcctcttt
     4861 tttgaaaatc ttggcaggcg tgcagcaatt ggacgatggc catttggtct gtcaaagcaa
     4921 tttgaaagtg gtgtatgtgc cgcaagaatc gttttttgat gatcagtcca atgtgtttga
     4981 tgtggtggcc gaaggcttgg gcgaactcaa acatcattta catgaatacc accgcatcag
     5041 cgcggctttg gccgagcaag cagaccatgc cgacttatta aagcagctca atcaattgca
     5101 agtggaaatt gaaagccgtg atggctggca atttgacagc ttaatcgtac aaaccttggc
     5161 gcaactgggt ttgcctgagg atgaattaat cggcaatttg tctggtggcc agaaaaaacg
     5221 cgtcgcctta gcgcaggctt gggtgcaaaa gccagattta ttgttgctgg atgaacccac
     5281 caaccatctg gacatcgaag ccattgaatg gctggaaacc ttgttgcaaa actttaatgg
     5341 cagcttggta gtgattaccc atgaccgccg cttcttggat gccattgcca cccgcattgt
     5401 cgaactcgat cgcggcatca tgcgcagcta tccaggcact ttcacccaat acaccgacat
     5461 caaaacccaa gaattggcag ccgaagccga acaaaccgct tgtttgacaa attccatgcc
     5521 caagaagaag tgtggat
//
LOCUS       DAOMVK010000097         5503 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_4079_length_5503_cov_2.833333, whole genome shotgun sequence.
ACCESSION   DAOMVK010000097 DAOMVK010000000
VERSION     DAOMVK010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 5503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5503
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4079_length_5503_cov_2.833333"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="PK856_04630"
     CDS             <1..781
                     /locus_tag="PK856_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01692.1"
                     /translation="KNKRANYIGVMFSGIGLALVVAVPFNTWVGEMQGWRYMYRFMAL
                     IDLLILVLLHNLSPALPLQHQNSITQGLRAFKNPKVWWVLLLGATVISGKVGVLTYAE
                     HIVMEITHLHGSLLPVIIMCVGLGMTTGSLLGGKLASLNLQKTLFGAICWVMVVMVLV
                     RLLAPDVHTVYLAFYFLGTITVFIPALQVLIIDYTQPYATLPSAAYHSALNCGNAAGP
                     LLSSVLIGAGYGYLSSTWVGLALAVCAAVIYVLGRTYLRPV"
     gene            complement(778..1098)
                     /locus_tag="PK856_04635"
     CDS             complement(778..1098)
                     /locus_tag="PK856_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01693.1"
                     /translation="MLFSVWVLTFLLSVGTGLYSSRRYLQPEPKRRGRHTVYQAIAGL
                     NYIICVLSFLLMCGYLAVQNVLDDAVPFGFLLITCLMSLFIASSALNKAEHIEIECAN
                     QSVG"
     gene            complement(1266..1754)
                     /locus_tag="PK856_04640"
     CDS             complement(1266..1754)
                     /locus_tag="PK856_04640"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HRL01694.1"
                     /translation="MSQQITLIAAVAKNDVIGINNLMPWHIKEDFKFFKEFTTGKPVI
                     MGRKTWESLMTRPLPNRRNIVITRQADYEAVGAEVVGDLQAAFALCTDVPEIVVMGGA
                     QIYAEALPLATDLRITEVDLNVDGDAYFPSIDASAWQELEREAHVSANGLAFSFVHYQ
                     QR"
     gene            complement(1880..2674)
                     /locus_tag="PK856_04645"
     CDS             complement(1880..2674)
                     /locus_tag="PK856_04645"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HRL01695.1"
                     /translation="MQQYHDLMRHVLEHGHDKGDRTGTGTRSVFGYQMRFDLSEGFPV
                     LTTKKLHLRSIIHELLWFLQGDTNIRYLKENGVSIWDEWADEEGNLGPVYGYQWRSWP
                     APDGRHIDQIATVIEQIKNNPNSRRMIVSAWNPALVDDMALPPCHAMFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMVAQVCGLQAGEFIHTLGDAHLYSNHFEQARL
                     QLERAPKDLPTMHINPEVKDLFGFKFEDFELVGYDPDPHIKAAVAI"
     gene            2919..3533
                     /locus_tag="PK856_04650"
     CDS             2919..3533
                     /locus_tag="PK856_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01696.1"
                     /translation="MSNPTHTRTHTPLRLRLSGQARLHFAQLMLWQILAFMALLAVFL
                     LHNCDILTFHSHPSNVSLLSQWQHILYQASGFDLLWHAVFSVAFLVLGKHISTNFNRT
                     LLPTYLVIEDNKLFWQTHGNGSRIDTVPLQSLDTVLFQGRDLDDLLQAASSDISVHLK
                     RRYHPTIIIRKDDFEQADALLQLLPRLMVTTHPKTKFQFFRQHM"
     gene            3681..4229
                     /locus_tag="PK856_04655"
     CDS             3681..4229
                     /locus_tag="PK856_04655"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HRL01697.1"
                     /translation="MLDREGYRPNVGIIIVNNRNQVFWGKRVREYNWQFPQGGIKPGE
                     SPEAAMYRELMEEVGLSPHHVKILGRTRDWLRYDVPSTWVRREWRGSYRGQKQIWYLL
                     RLTGRECDVYLRANNKPEFDAWRWHDYWAPVDEVIDFKREVYLGALQELARFLRPLET
                     WDAFLERKQATSPQTATISHPE"
     gene            4275..4763
                     /locus_tag="PK856_04660"
     CDS             4275..4763
                     /locus_tag="PK856_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01698.1"
                     /translation="MNNSMVHIALISLALSLLYLAFILGMMAWMANKAAKTTPKTIIN
                     IPIQANLLQTIANFAQHNGYKERPELSLGNEKVWRKGRGWLTSLTQLHFRLQADGNAE
                     LEVQEGANFLIKILFFPINSGSLLGLPVRQRKVKKLNQLLHSLNAAPIEFPKGKRIKV
                     KK"
     gene            4921..>5503
                     /locus_tag="PK856_04665"
     CDS             4921..>5503
                     /locus_tag="PK856_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRL01699.1"
                     /translation="MSDLQQYDESSVTVLKGLEPVKQRPGMYTRTDNPTHICQEVIDN
                     AADEALGGYADTIAVTIHSDGSMSISDNGRGIPVGLHPTENVSVVELVFTRLHAGGKF
                     NKKDGGAYAFSGGLHGVGVSVTNALSTRLEVQVKREGTVSQIVFSGGDIIEPLTEIGT
                     CAKKDTGTTVRVWPDPQYFEDAQYSIAEIERLLR"
BASE COUNT         1497 a         1371 c         1196 g         1439 t
ORIGIN      
        1 caaaaacaag cgtgccaact acatcggtgt gatgttttca ggcataggct tggcattggt
       61 tgtggcggtt ccgtttaata cgtgggtagg agaaatgcaa ggctggcgct atatgtaccg
      121 cttcatggcc ttgattgatt tgttgatttt ggtcttactg cacaacctca gccctgcact
      181 gccattacaa caccaaaact caatcacaca aggcttacgg gctttcaaaa accccaaggt
      241 ttggtgggta ttgcttctgg gcgcaacagt catcagtggc aaagtggggg tgttgactta
      301 tgctgagcac atagtgatgg agattaccca cctacatggc agcctattgc cagtcatcat
      361 catgtgtgtg ggattgggca tgacaaccgg cagtctattg ggtgggaaac tggccagctt
      421 aaacttacaa aaaaccctgt ttggggcaat ctgttgggtg atggtggtga tggtattggt
      481 acggctattg gcaccagatg tgcacaccgt gtatttagca ttttactttt tgggcaccat
      541 cactgttttc atacccgcct tacaggtgtt aatcatagat tacacccaac cttatgccac
      601 gcttccgtct gctgcctacc actccgcgct caattgtggc aatgccgcag ggccattgct
      661 cagcagcgtt ttgataggag caggttatgg ctatctttcc agcacttggg taggtttggc
      721 tttggctgtg tgcgcagcag ttatttatgt gctgggtagg acttatttgc gccctgttta
      781 gcccaccgat tgattggcac attctatttc aatgtgctca gccttgttca gagcggaact
      841 ggcaatgaac aaactcatga gacaagtaat cagcagaaaa ccaaaaggca ccgcatcatc
      901 caagacattt tgcaccgcca aataaccgca catcaacaga aaactaagca cacaaatgat
      961 gtaattcaaa cctgctatgg cttgatacac cgtgtgccgc cctctgcgct tgggttcggg
     1021 ctgcaagtaa cgacgactgc tgtacaagcc cgtacccaca cttaacaaaa atgttaacac
     1081 ccagaccgag aacaacattt ttaggcctct taaaatagaa tgactgctta aactgtaaac
     1141 atcatatctg tttttgttaa gaagcgtgga gattgcagct aaacgatgca ctcttttgac
     1201 caacggaaag cggcctgtaa atatacttac aggccgcttc aagatcacaa tccaatttta
     1261 gatggctatc tttgctggta atgcacaaaa gaaaatgcca aaccattggc gctgacatgt
     1321 gcctctcgct ctagctcttg ccaagcgctg gcatcaatgc tgggaaaata cgcatcacca
     1381 tccacgttca aatctacctc cgtgatgcgc aaatccgtgg ccaaaggcaa agcctccgca
     1441 taaatttgcg ccccgcccat cacaacgatt tcaggtacat cagtacataa ggcaaaagcg
     1501 gcctgtaaat cccccaccac ttccgccccg acagcctcat agtcggcttg gcgtgtaatc
     1561 acgatattgc ggcgattggg taatggtcta gtcatcaatg actcccaggt ttttcgcccc
     1621 atgattactg gttttccagt agtaaattct ttaaagaact taaaatcttc tttaatatgc
     1681 caaggcatta aattgttaat accgattaca tcgtttttcg cgactgcggc tattaaagta
     1741 atctgctggc tcacagcaac accccaatga ctcttaaaat ttaagggtga attctatatt
     1801 gaattccagc gattcacaaa tgaattgctc atcgcgatag caaaatgagc attttactcg
     1861 tattcattca atttatatcc tatatagcca ccgctgcttt gatgtgcggg tcggggtcgt
     1921 agcccaccaa ttcaaaatct tcaaacttaa agccaaacaa atctttgacc tcaggattga
     1981 tgtgcatggt gggcaagtct ttcggcgcac gctccaactg caaacgcgct tgctcaaagt
     2041 ggttgctgta caaatgggca tcacccaaag tatgaataaa ctcaccggcc tgtaagccac
     2101 acacttgtgc caccatcatg gtgagcaagg catagctggc tatgttaaac ggcacgccca
     2161 agaaaatatc ggcactgcgt tggtacaatt gacagctgag tttgccatcg gccacataaa
     2221 attggaacat cgcatgacac ggcggcaaag ccatgtcgtc taccaaagca ggattccatg
     2281 ctgagactat catgcggcgt gagttagggt tatttttaat ctgctctatc accgtcgcga
     2341 tttggtctat atggcgacca tctggtgcag gccaagagcg ccattggtag ccataaacag
     2401 ggcctaaatt gccctcttca tccgcccatt catcccaaat cgacacccca ttttctttca
     2461 aataacggat gttggtatcg ccttgcaaaa accacagcaa ttcatgaatg atggaacgca
     2521 agtgcaattt tttggtggtg agcacaggaa aaccttcgct caaatcaaaa cgcatttgat
     2581 aaccaaacac cgaacgcgtg cctgtgcccg tgcgatcgcc tttgtcatgg ccgtgctcta
     2641 atacatggcg cattaaatcg tggtattgct gcatgtttaa atatccatag tctgatgctc
     2701 agttctaatc ctcggtattg tacccgaatc acttgcccct ataaaaccat gtattttcca
     2761 gtaattaagt tcaaacaagc ggcctgtaaa ccactcaaaa taatgacttt cttttcaagc
     2821 aattaaataa aaatacgtaa ttttatggct gctggcacga tgcttgctaa ggcacaactg
     2881 aatcagaaaa aataataaca ttcaggcttg gagccatcat gtcaaatccc acccatacac
     2941 gcacacatac cccactgcgt ttgcgcctat ctgggcaagc tcgtttgcat tttgcgcaat
     3001 tgatgctgtg gcaaatcttg gcgtttatgg ctttgttggc ggtattttta ctgcataatt
     3061 gtgacatttt gacatttcat tctcacccat cgaatgtaag cttgctgtct caatggcaac
     3121 acattttgta ccaagcgtca ggatttgatt tgctttggca tgccgtattc tcagtggcat
     3181 tcttggtatt gggcaagcac atcagtacca attttaaccg caccctactg ccaacgtacc
     3241 ttgtcattga agacaataaa ttgttttggc aaacccatgg caatggcagc cgcatcgaca
     3301 cagtgccttt acaaagcttg gatacggtgt tgtttcaagg ccgtgacttg gatgatttat
     3361 tacaggccgc ttcttctgac atcagtgtcc acttaaagcg ccgctaccac ccaaccatca
     3421 tcatccgcaa ggacgatttt gagcaagcag atgccttgtt gcaattgctg ccacgcttaa
     3481 tggtcacaac ccaccccaaa accaaatttc aattctttcg tcagcatatg taaatcccca
     3541 cctccaaaaa tgaatttaaa catttgtttt atagaagccc ctacacgaat acgccaggct
     3601 tttcaactat agatgtgtgg ctgctttcag gcatttatgc gacaataaat cccaatttca
     3661 ttcctaattg ggcgaagagc atgttagaca gagaaggtta tcgccccaat gttggcatca
     3721 tcatcgtcaa caaccgcaat caagtgtttt ggggtaagcg cgttcgcgaa tacaactggc
     3781 aatttccaca aggtggcatc aaaccaggtg aaagccctga agctgccatg taccgtgagt
     3841 taatggaaga agtgggtctg tcaccccacc atgtcaaaat attgggacgc acacgcgact
     3901 ggttgcgcta cgatgtaccg tctacttggg tcagacgcga atggcgtggc tcttaccgcg
     3961 gccaaaagca aatttggtat ttattgcgct taacgggtcg tgaatgtgat gtgtatttac
     4021 gcgcgaacaa caaacctgaa tttgatgcat ggcgttggca cgattactgg gctcctgtcg
     4081 atgaggtcat agactttaag cgcgaagtgt atttgggcgc actgcaagaa ttggcgcgtt
     4141 ttttgcgccc cctagaaact tgggatgcgt ttcttgagcg caaacaagcc acctctccac
     4201 aaactgccac aatcagccac cccgaataac cacaatgatg gccatgccat cattgttttt
     4261 ttcacaggaa tgcaatgaac aacagcatgg ttcacatcgc cttaatcagc ttggctttat
     4321 ccctgctcta tctagcattc atcttgggca tgatggcatg gatggcgaat aaagccgcca
     4381 aaaccacacc caaaaccatc atcaacattc ccatccaagc gaacttgctg caaacaattg
     4441 ccaactttgc ccaacataat ggctataaag agcggcctga actcagtttg ggcaatgaaa
     4501 aagtttggcg caaaggcaga ggttggctca ccagcttgac ccaattgcat tttcgtttac
     4561 aggccgatgg caatgctgaa ctggaagtac aagaaggagc gaatttctta atcaaaatcc
     4621 tgttctttcc catcaactca gggagcttgc tcggtttacc cgtcagacaa cgcaaggtaa
     4681 aaaaactcaa ccaactcctg cattccttaa acgcagcgcc tattgagttt cccaaaggca
     4741 aacgcattaa ggtaaaaaaa taggccgcat ggccttaaag atttaagatg ggcgcaagtt
     4801 ttgtgcgccc ctgattgctc ggctgggctg tttcacaata ccagcaaacg acgcggcatt
     4861 tgtggaaacc acttaaaaac cgttacacta atcacttatt cagatgattt aacaccccat
     4921 atgtcagact tacagcaata cgatgaatcc agcgtcaccg tcttaaaagg cttagagcct
     4981 gttaagcaac gccctggtat gtacacccgc accgacaacc ctacccacat ttgtcaggaa
     5041 gtcatagaca atgctgccga tgaggcattg ggtggctatg ccgacaccat cgccgtcacc
     5101 atacacagcg atggctccat gagcatcagt gacaatggtc gcggcatccc agtgggctta
     5161 cacccgactg aaaatgtcag tgtggtggag ttggtgttca cccgtttgca tgctggcggc
     5221 aaattcaaca aaaaagacgg tggcgcttat gccttctcag gcggtttgca cggtgttggt
     5281 gtttctgtga ccaatgcctt atccacccgc ctagaagtac aagtcaaacg cgaaggcacg
     5341 gtttcccaaa tcgtgttttc tggtggtgac atcattgaac ctttgactga aatcggcact
     5401 tgcgccaaaa aagacacggg tacgactgtg cgcgtgtggc cagacccaca gtattttgaa
     5461 gatgcgcaat actccatcgc cgaaatcgaa cgcttgttgc gcg
//
LOCUS       DAOMVK010000098         5494 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_4086_length_5494_cov_4.643501, whole genome shotgun sequence.
ACCESSION   DAOMVK010000098 DAOMVK010000000
VERSION     DAOMVK010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 5494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5494
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4086_length_5494_cov_4.643501"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..1408)
                     /locus_tag="PK856_04670"
     CDS             complement(50..1408)
                     /locus_tag="PK856_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRL01700.1"
                     /translation="MNKLPTLLALALCSMAWTAQAETIPATPLQAATPVKSHNINIQR
                     WQLDNGAKVLLVERHELPMVDIDVAFDAGTRRDAANKIGVADYAGALMDMGAGDLSEE
                     DIRKQSSDLAVTVSSYAGLERAGVRIRSLSAAPTLTPTLALANTILTQPRYEAAVLKR
                     EQDRSVLSLKQNETNPQFLGSRAMTRLNYPKHPYGYSAWETEKTIRAITVADLQRFHT
                     QYFRPQTAVISIVGDMNRSQAEAMAKTLLAGLPQEAIKLKPVAAVPVKGGQTTEIAHP
                     ASQAHLSLGLPVFTRDDPDYFPLLIGNYTLGAGGFDSLLMQELRDKRGYTYGAYSNLE
                     PLAQKGEFTISMSTKKASAADSLKVTRQVVQDYIAQGPTDAQLQQAKANIIGGFPMRF
                     DTNAKLLSWLGTIGFYDLPSDYLDTYTQKVQALTTAQIRDAWQRRIKVEDLNVVIVGG
                     EK"
     gene            complement(1405..2739)
                     /locus_tag="PK856_04675"
     CDS             complement(1405..2739)
                     /locus_tag="PK856_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRL01701.1"
                     /translation="MSLRVWTLAFACAAISAPVFAETVSKTLDNGLKIIVREDARAPV
                     VVTQLWYKVGSVDEEAGKTGLSHVLEHMMFKGTPTVPSGEYSKRITAMGGELNAYTNR
                     DETVYYQTVAKQHLPEVLQMEADRMVNLGFSNKDYVNELEVVKEERRLRTDDSPSGRL
                     YEKLYQTAFTTSGNKSPIIGSMDDLTHMTGDDARAWYRKWYAPNNATMVIVGDVNAKQ
                     AIKQVENAFGALKPSALPSRALPAEPKQTQARQAEVSAPSELPIVALGYQVPQLKNIN
                     DKLPYALDTLADVLDGNAASRMEKNLVRAKPIALSAGASYSLLTRADTVFVLSGAPAQ
                     GVSVPQLIAALKGEVADIAKNGVSEEELARIRNQRAASETYAKDSMATQASLMGDLES
                     KGFGYQDEDEMRRRLNAVTAAEVQQAAQFLSDETVTQVILKPEALNPKGGKQ"
     gene            2806..4536
                     /gene="ftsY"
                     /locus_tag="PK856_04680"
     CDS             2806..4536
                     /gene="ftsY"
                     /locus_tag="PK856_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRL01702.1"
                     /translation="MFSFFKKKFKKSEPEQKQPELEQDSPLPEASDSNKEAVSQEAQA
                     ELVNIPEPSAAPIVAETAPSQTEPKQSPESLAALNFDDLDAEKAAEPAPVEAEVVVST
                     PAPTATPTEAPTPAPTQALTPEPTLAPTAMPTTAPTPAPTQAATVAPTPEPTPESTTT
                     PVLEEMRPVNEATTATTIETAAETEEAAESAEAPQGWARGITPNKALSGQLETDAQAT
                     TETAGEETSFFTQNWTRNIVAEDKEIVVKPTAAPVATAAPVVQQDPNQNFEPGQTSGW
                     TARLKQGLSKSRDKLGKSLAGVFGGAEIDDDLYEELETVLLTSDMGVEATEHLLEDVR
                     QRVSLKGLKNPEELREALKDALVDLIAPLQKPLEIPAETQPFVIMMAGVNGAGKTTSI
                     GKLATHYQRQGKSVLLAAGDTFRAAAREQLIEWGARNNVTVISQATGDSAAVCFDAMH
                     AAQARGTDIVLADTAGRLPTQLHLMEEIKKVKRVMQKSMDTAPHEVILVLDANIGQNA
                     VNQVIAFDDALGLTGLILTKLDGTAKGGVIAALAKTRPIPIRFIGVGEGIEDLRPFNA
                     RDYAEALLGD"
     gene            4569..5426
                     /locus_tag="PK856_04685"
     CDS             4569..5426
                     /locus_tag="PK856_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRL01703.1"
                     /translation="MTRTRAFINMHIATVFFGLSGVFAALIPASAISVVWGRTAIAVV
                     VLFGVCLLKKRLPWHGVSKSEIAQLLGIGCLLGVHWWSFFYAIQLGGVAIGTLGFSCF
                     PAAIAIIEAVVFKDRISKLELALIGLITVGLILVTPSFDFANQATIGLLWGIFSGVIY
                     AVIAACNRVGAAKRQASSLQACWWQFFAISVVTAFAVPQLSGLGGMGWVWLFGLGVLC
                     TGLAYWLFIDSLSAINARTAAMIFALEPVYAIAIAWAFLHDVPSLRMLLGGSLIIAAV
                     VAAARFKSS"
BASE COUNT         1221 a         1457 c         1494 g         1322 t
ORIGIN      
        1 ttgctgtgac gactgttgtt gcgctatgtg tttacaggcc gcttaaaacc tatttttcac
       61 cgcccactat caccacattc aagtcttcta ccttgatgcg gcgctgccat gcatcgcgga
      121 tttgggcggt ggtcaaagcc tgtacttttt gggtgtaagt gtccaagtaa tcgcttggta
      181 agtcgtaaaa accaatggtg cccaaccatg acaacagctt ggcattggtg tcgaagcgca
      241 tggggaagcc gccgatgatg ttggctttgg cctgttgcaa ttgcgcgtcg gtcgggcctt
      301 gggcaatgta gtcttgcacc acttggcgcg tgactttcaa tgagtcggcg gcactggctt
      361 ttttggtgct catgctgatg gtgaattcgc ctttttgcgc caatggctcc aaattgctgt
      421 aggcgccata ggtatagccg cgtttgtcgc gcaattcttg catcagcaag ctgtcaaagc
      481 cgcctgcgcc caaggtgtaa ttgccgatga gcaaagggaa gtaatcgggg tcgtcacgcg
      541 tgaacacggg caagcccaaa gacaaatggg cttggctggc tgggtgggcg atttcggtgg
      601 tttgcccgcc ttttacaggc acggcggcca cgggtttcaa cttgatggct tcttgtggca
      661 aacccgccaa cagggttttc gccatggctt cggcttggct gcgattcatg tcgcccacaa
      721 tcgaaatcac cgcggtttgc gggcggaaat actgtgtatg gaaacgctgc aaatcggcaa
      781 ccgtgatggc gcgtatggtt ttttcagttt cccaagcgct gtagccatag gggtgttttg
      841 gataattcaa acgcgtcatg gcgcgactgc ccaaaaactg gggattggtt tcgttttgct
      901 ttaaagacaa aaccgaacgg tcttgctcgc gcttgagcac ggcggcttca taccgcggct
      961 gggtcaaaat ggtgttggcc aaggccaaag tcggcgttaa ggtgggcgct gcgctcaggc
     1021 tgcgtatgcg cacacccgcg cgctccaagc ctgcataact ggacaccgtc accgccaaat
     1081 cgctgctttg cttgcggatg tcttcttctg acaagtcgcc tgcgcccatg tccatcaacg
     1141 cgcccgcata atcggccacg ccgattttgt tggcggcatc gcggcgtgtg cccgcatcaa
     1201 aagccacatc aatgtccacc atgggcaatt cgtggcgctc taccaacaag actttggcgc
     1261 cattatccag ctgccaacgc tgtatgttga tgttgtggct ttttacgggc gtagcggcct
     1321 gtaaaggtgt ggcaggaatg gtttcggctt gggctgtcca tgccatgctg cacaaagcca
     1381 gagccaataa ggtagggagc ttgttcattg tttgcctcct tttggattca aggcttcggg
     1441 ttttaaaatc acttgagtca cggtttcatc gcttaaaaat tgggcagctt gctgcacttc
     1501 tgcggccgtt acggcattca agcggcggcg catttcgtct tcgtcttggt agccaaagcc
     1561 tttgctttcc aaatcaccca tcaatgaagc ttgggtggcc atcgagtctt tggcataggt
     1621 ttctgaggcg gcgcgttggt tgcgaatgcg cgccaattct tcttcgctca ccccgttttt
     1681 ggcaatgtcg gccacttcgc ctttcaaagc cgcaatcaat tgcggcacgc tcacgccttg
     1741 cgcgggtgcg cccgaaagca caaacacggt atcagcgcgg gtaagcaagg aatatgaagc
     1801 ccctgcggac aaggcgatgg gcttggcgcg caccaaattt ttctccatgc ggctggcagc
     1861 attgccatcg agcacatcgg ccaaggtatc gagcgcatat ggcagcttgt cgttgatgtt
     1921 tttcaattgc ggtacttggt agcccaaggc cacgatgggc aattcagagg gagcagacac
     1981 ttcggcttgt ctggcttggg tttgtttggg ctcggcaggc aaagcgcggc taggcaaggc
     2041 tgatggcttc aatgcaccaa aagcgttttc cacctgtttg atggcttgct tggcattcac
     2101 atcgcccacg attaccatgg tggcgttgtt gggcgcatac catttgcgat accacgcgcg
     2161 cgcgtcatcg cccgtcatgt gggttaaatc gtccatagaa ccaatgatgg gcgatttgtt
     2221 gccactggtg gtgaaggcgg tttggtagag tttttcatac aagcggcctg aagggctgtc
     2281 gtcggtgcgc aaacggcgct cttctttcac cacttccagc tcattgacat aatctttgtt
     2341 gctaaaaccc aaattcacca tgcggtcggc ttccatttgc aacacttcag gcaaatgctg
     2401 tttggccacg gtttggtaat acacggtttc atcgcggttg gtataggcat tgagctcgcc
     2461 gcccatggcg gtgatgcgtt tggagtattc gccagaaggc acggtgggcg tgcctttgaa
     2521 catcatgtgc tccaacacat ggctcaaacc tgttttgcct gcctcttcat ccacgctgcc
     2581 cactttgtac caaagctgtg tgaccaccac aggggcacgg gcatcttcgc gcacgatgat
     2641 tttcaagccg ttgtccagtg ttttgctgac cgtttcagcg aaaacaggcg cgcttatcgc
     2701 tgcacatgca aatgccagcg tccaaactct tagagacatg attaatcctt gatttttacg
     2761 ctacaatagg tgttactcaa gcatatatgt tgattgaaag cactcatgtt cagtttcttt
     2821 aagaaaaaat ttaaaaaatc cgaaccggaa caaaaacaac cggaattaga gcaagacagt
     2881 cccttgccag aagcaagcga ttctaacaag gaagccgtgt cacaggaagc acaggccgag
     2941 cttgttaaca tacctgaacc tagcgctgcc ccgattgtgg cagaaactgc cccaagccaa
     3001 actgagccaa agcaaagccc tgaaagcttg gccgcactga attttgatga tttggacgca
     3061 gaaaaagccg cagaacctgc tccagtggag gcagaagtgg tggtcagcac tccagcgcct
     3121 accgcaaccc caacggaagc accgacacca gctcctacac aagcgctcac gcctgaaccg
     3181 acattggcgc cgactgcgat gccaaccaca gcgccgacac ctgcacccac tcaagcagcc
     3241 acagttgccc caacgccaga acccacccca gagtctacga cgacacctgt gcttgaggaa
     3301 atgcggcctg taaacgaagc gacaacagca accacgattg aaaccgcagc tgaaactgaa
     3361 gaagcagcag aatcagcaga agcgccacaa ggttgggcgc gcggcattac gcctaacaaa
     3421 gccttgagcg gccaattaga aaccgatgca caggcaacaa cggaaactgc tggcgaagaa
     3481 acctcgttct ttacccaaaa ctggacgcgc aacattgtgg ccgaagacaa agaaattgtc
     3541 gtgaagccta ccgctgcacc cgtggccact gccgcgcctg tggtacaaca agacccaaat
     3601 caaaatttcg agccaggcca aaccagtggc tggaccgcgc gcttaaaaca aggcttgagc
     3661 aaatcgcgtg acaaattggg caaatcgttg gcaggtgtgt tcggcggtgc ggaaatcgac
     3721 gacgatttgt acgaagaatt ggaaaccgtg ctcttaacca gcgacatggg cgtggaagcg
     3781 accgagcatt tgctggaaga cgtgcgccaa cgcgtgagct tgaaaggctt gaaaaaccct
     3841 gaagaattgc gcgaagcctt aaaagacgct ttggtcgatt tgatagcccc cttgcaaaaa
     3901 cccttggaaa tccctgccga aacccagcct tttgtcatca tgatggcggg tgtaaacggc
     3961 gcgggcaaaa ccacttccat cggcaaactc gccacccatt accaacgcca aggcaaatcg
     4021 gtcttgctcg cagcaggcga taccttccgc gctgcagcgc gtgagcaatt gattgaatgg
     4081 ggcgctcgca acaatgtcac cgtgatttca caggccacag gcgattctgc cgccgtgtgt
     4141 ttcgatgcca tgcacgcagc acaagcgcgc ggcaccgaca tcgtgttggc cgacaccgcg
     4201 gggcgcttac ccacgcaatt gcatttgatg gaagaaatca aaaaggtcaa acgcgtgatg
     4261 caaaaatcca tggatacggc gccacacgaa gtgattttgg tactcgatgc caacatcggc
     4321 caaaacgcgg tcaaccaagt gattgcgttt gacgatgcct tgggcttaac aggcttgatt
     4381 ctgaccaaac tcgatggcac agccaaaggc ggcgtgattg cagccttggc caaaacccgc
     4441 ccgattccca tccgctttat tggcgtgggt gaggggattg aggatttgcg tccattcaat
     4501 gcgcgtgatt atgccgaagc cttgctaggc gattgataag cggcctgtaa acctgtgtga
     4561 aagcccctat gacacgtacc cgtgccttca tcaacatgca catcgccacc gtcttttttg
     4621 gtttgtcggg tgtgtttgcc gccttgatac ccgcttcggc catttcggtg gtgtgggggc
     4681 gcactgctat tgcggtggtg gtgttgtttg gggtgtgcct gctgaaaaaa cgcctgcctt
     4741 ggcatggcgt ttctaagtct gaaattgccc aattattggg cataggctgc ttattgggcg
     4801 tgcattggtg gtcgtttttc tatgccatac aactcggtgg tgtcgccata ggcacgctgg
     4861 gcttttcttg ttttcccgcc gccattgcca tcattgaggc cgtggtgttc aaagaccgca
     4921 tcagcaaact ggaattggcc ttaatcggct tgattaccgt tggtctgatt ttggtgaccc
     4981 ccagttttga ctttgccaac caagccacca ttgggctgtt gtggggcatc ttctcaggcg
     5041 tgatttacgc cgtgatagcg gcctgtaacc gcgtgggcgc ggccaaacgc caagccagtt
     5101 cgttgcaagc gtgttggtgg caatttttcg ccatcagcgt ggtcacggca tttgctgtgc
     5161 cacaattaag cggcttgggt ggcatgggtt gggtatggtt attcggcttg ggcgtgttgt
     5221 gcactggttt ggcgtattgg ctgtttatcg acagcttgtc tgccatcaat gcccgcaccg
     5281 ccgcgatgat tttcgccctc gaacctgtgt acgccatcgc cattgcttgg gcttttttac
     5341 acgatgtacc gagtttgcgc atgttattgg gcggcagctt aatcattgct gccgtcgtgg
     5401 cggctgcccg tttcaaatcc tcttgaaagg cactgatgat acagtttgaa caagtcagta
     5461 aaacctatcc aggcggccat gccgcgttga aaaa
//
LOCUS       DAOMVK010000099         5486 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_4089_length_5486_cov_3.551648, whole genome shotgun sequence.
ACCESSION   DAOMVK010000099 DAOMVK010000000
VERSION     DAOMVK010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 5486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5486
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4089_length_5486_cov_3.551648"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(365..1384)
                     /locus_tag="PK856_04690"
     CDS             complement(365..1384)
                     /locus_tag="PK856_04690"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HRL01704.1"
                     /translation="MYSLIKPLLFQFDAEKAHHMTLENLQRGHRFGLLSHFGNKVMPC
                     PSTLMGLALKNPVGLAAGLDKNGEYIDALAALGFGFIEIGTITPRPQDGNPKPRLFRL
                     PEHQAIINRMGFNNHGVDALIANVKRAKFQGVLGINIGKNATTPIENAVDDYLTCLTK
                     VYDHASYITVNISSPNTKNLRALQSEDELSKLLGTLKEEQARLHQQRGRYVPMAVKIA
                     PDLNLEEIEAIAQVVTQVEMDGVIATNTTVDKSSLGNHPLAGEAGGLSGLPVCAQSEY
                     VLQHLVYALKGKLPVIGVGGIVSGQDAATKKRLGASAVQVYSGMIYQGPSLIADCVRE
                     WVHTR"
     gene            complement(1434..2348)
                     /locus_tag="PK856_04695"
     CDS             complement(1434..2348)
                     /locus_tag="PK856_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRL01705.1"
                     /translation="MSLHTLAAFRSHLSQFLERMQQRNLSELSITAYRRDLQQLCDLL
                     AEKLDETEDLTAMHLRHVLMRLSQKGLNPRSLGRKTSVWKQYAAFLRQHQGWETDLSV
                     GLKAPKTNTRLPKAVPAEPLNQWFSHGADKDDAMAIRDQAMFELLYGCGLRLSELRAL
                     DINDVQLEAGWVSVQGKGNKQRQVPLGLQARLAIEAYLPLRQASSAESALFTSRLGTR
                     IGQRQIQLRLDAWAQRQNSDRHLSPHMLRHSYASHVLQSSQNIRAVQELLGHKQLSTT
                     QIYTSLDFTHLSEVYDAAHPRAKKHKPK"
     gene            complement(2345..2761)
                     /locus_tag="PK856_04700"
     CDS             complement(2345..2761)
                     /locus_tag="PK856_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01706.1"
                     /translation="MRTYPANSPQARARILAMCLLADSDLDEEELESLSNKNVYDLLD
                     LTALEFMQVLRDYLHDLSNKLNTPDARVSMLEPEQVNAILAEVTERPSRIALLATALS
                     LCKGDHALNPAELALFQHIMGLWQLDLTDLEIEVSR"
     gene            complement(2774..3205)
                     /locus_tag="PK856_04705"
     CDS             complement(2774..3205)
                     /locus_tag="PK856_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRL01707.1"
                     /translation="MSRIKIDLPQEWHFQTSITVQIGDINYGNHLSNDAYLRLAHEAR
                     LRFLKHHGYTEMNIDGAGLIMADAAIQFKNQAFHGDELNIRVAVTDIGRAGFTLIYLL
                     ERPSDGLQIGRIKNGMVFYDYQQQCVANTPAAFSAAFATID"
     gene            3282..3734
                     /locus_tag="PK856_04710"
     CDS             3282..3734
                     /locus_tag="PK856_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRL01708.1"
                     /translation="MYQHILVPFDDSQAALVALVEACKVGRLTGGTLHIVHVVDLAQF
                     SWSGASFLRSEELAMASADTAKAVFERAEPIMAEYADVQAEHTVLEAVGEKVASLLVN
                     KISEWQCDLVVMGTHGFSGVKHLLMGSVAQDVIRQAGIPVLILRVAAP"
     gene            complement(3806..4711)
                     /locus_tag="PK856_04715"
     CDS             complement(3806..4711)
                     /locus_tag="PK856_04715"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRL01709.1"
                     /translation="MAKQRYFLGCVFVVLAASCWGLIGLLGAKLNQAGFSGLEVASLR
                     ITLASVVLILVSPFVLNSLRVFQRKQLPLLSMQSLLGVFLMTVCYFLAVQHIGAALSV
                     ALLYTAPVWSMLLAHFLLKERMSLSGVMLTLITVVGVALCLNTSAPLNWTGIAFGLLS
                     GFSYACFGVLGKKALNNTPPTILLYSSVLISGMAMLFMPFFHSAMQKMFTDMAPNFWM
                     VALMIAVVGTLLPYTLYTKALQWMPATRAQVLTIFEPLTAVLLAAVILHEPLGWPQYV
                     GIAMILASALINALAQPQNSIPSNA"
     gene            complement(4798..5346)
                     /gene="sodC"
                     /locus_tag="PK856_04720"
     CDS             complement(4798..5346)
                     /gene="sodC"
                     /locus_tag="PK856_04720"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HRL01710.1"
                     /translation="MSSHKYPIYFALVCSSFILAACQSTASSNSVQAEVNSVSSQGVG
                     LSIGHVSFKDTPQGLLIQPQLSALPPGPHGFHIHGTPSCATANNAEGKLTAAQAAGGH
                     LDPHNTGKHLGPQAHGHAGDLPVLQVAANGTATTAVLAPNLHLSDVRQRAIMVHAGSD
                     NYSDQPQALGGGGARIACGIIP"
BASE COUNT         1394 a         1291 c         1461 g         1340 t
ORIGIN      
        1 cgacaacaaa cccgcaacaa tggcccctat catcagggac aacacgaccg atacgcgcat
       61 caaactcaaa atcacaacca cggatacagc aatcacaact gaattcattt ggtttccttt
      121 gcaatgcgtt gatgacttca aaatatgaaa tcatgctaaa agtgagcact gcaacaagtc
      181 aataaatttg tctatacaag taaattcaaa tttattaaac cattgaatta taatgaacaa
      241 attttttgta aaaaaagaca attgaaatta tatagcaaaa ataagctcta aatgttcaaa
      301 aagcgtcctg taagacaaat aggacgcttt ttgacaaatg aatattggta gggcaagagc
      361 agaatcagcg ggtatgtacc cattcacgca cgcaatcggc aatcaaactt gggccttggt
      421 aaatcatgcc gctgtacacc tgtaccgcac tggctcccaa acgcttttta gtggcggcat
      481 cttggccaga aacaatgcca cccacaccga tgacaggcaa tttgcctttc aaggcataca
      541 ccaagtgttg caacacatac tcgctttgtg cgcacacagg caagccagat aagccaccgg
      601 cttcacctgc caatgggtga ttgcccaatg aggatttgtc tacggtggtg ttggtggcaa
      661 tcacaccatc catttccact tgggtcacca cttgggcaat ggcttcgatt tcttccaaat
      721 tcaaatcagg ggcaattttt accgccatcg gtacatagcg accgcgttgt tggtgcaaac
      781 gtgcttgctc ttctttcagg gtgcccaata atttgctcaa ttcgtcttca ctttgcaagg
      841 cgcgcaggtt tttggtattg ggcgaagaaa tgttaaccgt gatgtagctg gcgtggtcgt
      901 agactttggt caaacaggtg aggtagtcat ccacggcatt ttcaatcggg gtggtggcat
      961 ttttaccaat gttaatgccc aagacgcctt ggaatttggc gcgtttgaca ttggcaatca
     1021 acgcatcgac gccgtggtta ttgaaaccca tgcggttgat gatggcttgg tgctcaggca
     1081 ggcggaacaa acgtggcttg ggattgccgt cttgtggacg tggggtgatg gtgccaattt
     1141 cgatgaaacc aaaacccaaa gccgccaatg catcgatgta ttcgccattc ttgtccaaac
     1201 ctgcggccaa gccaactggg tttttcaagg ccaaacccat taaagtgctg gggcagggca
     1261 tgactttgtt gccaaaatgg ctgagcaaac cgaagcggtg cccacgttgc agattttcta
     1321 gggtcatgtg gtgggctttt tcagcgtcaa attgaaagag cagcggttta atcagcgaat
     1381 acataacaag cggcctgaaa acaatgaaca gccgccatta taagcgatgg tgcttatttg
     1441 ggcttgtgct ttttggcacg aggatgggcg gcatcgtaga cttcagacaa gtgggtaaag
     1501 tccaggctgg tatagatttg cgtggtggaa agctgtttgt gaccgagcaa ttcttgcaca
     1561 gcgcggatgt tttgcgagct ttgtaacaca tgcgaggcat agctgtgacg cagcatgtgc
     1621 gggctcaaat gcctgtcgct gttttgtcgc tgtgcccaag catcgaggcg caattggatt
     1681 tggcgttggc ctatgcgcgt gcccaagcgg gacgtgaaca aagccgattc agcgcttgag
     1741 gcttgtcgca gcggcaaata ggcttcaatg gccaagcgcg cttgcaagcc aagcggcacc
     1801 tgtctttgtt tattgccttt gccttgcacg ctgacccaac ctgcttccaa ttgcacatcg
     1861 ttgatgtcca aagcgcgcag ctcagacaag cgcaagccgc agccatacaa cagttcaaac
     1921 atggcttggt cgcgtatggc catggcatcg tctttgtcgg cgccatgact gaaccactga
     1981 ttcaggggtt cagcaggaac ggctttgggt aggcgcgtgt tggtcttggg ggctttcagg
     2041 ccgacactca aatcggtttc ccacccttgg tgttggcgca gaaaggccgc atattgtttc
     2101 cacacgctgg ttttgcgccc cagactgcga ggattcaggc ctttttggct caagcgcatc
     2161 aacacatggc gcaaatgcat cgccgtcaaa tcctcagttt catccaattt ttcagccaat
     2221 aagtcacaca actgctgcaa atcgcgccga taggcggtga tgctcagttc agacaaattg
     2281 cgctgttgca tgcgctcaag aaactgcgac aggtgtgagc gaaatgcggc caacgtgtgc
     2341 aggctcatcg cgaaacttcg atttccaaat cggttaaatc caattgccac aagcccatga
     2401 tgtgctgaaa caaagccaat tcagcaggat tcaaagcatg gtcgcccttg cacaaagaca
     2461 aggcggtggc caataaggcg atgcgactgg ggcgttcggt gacttcggcc aaaatggcat
     2521 tcacttgttc tggctcaagc atggacacac gggcgtctgg tgtgttgagt ttgttgctca
     2581 agtcatgcaa atagtcgcgc aacacttgca taaattccaa agcggttaag tccagcaagt
     2641 catagacgtt tttattgctc aagctttcca actcttcttc atccaaatca ctgtctgcca
     2701 ataagcacat ggccaagatg cgcgcacggg cttggggact gttggctggg taggtacgca
     2761 tgtgtgggtt tccttaatcg atggtcgcaa acgctgcgct gaatgcggca ggggtgttgg
     2821 caacacattg ttgttgatag tcgtaaaata ccatgccgtt tttgattcga ccaatttgta
     2881 ggccatcaga tgggcgttct agcaggtaaa taagggtaaa gcccgcgcga ccaatgtcgg
     2941 ttactgcgac tcggatgttg agttcatcgc catgaaaggc ttggtttttg aactgtatgg
     3001 ccgcatcggc catgatcaag cctgcgccat ctatgttcat ttcggtgtaa ccatggtgtt
     3061 tcaagaagcg caatcgggct tcatgggcca aacgcaagta agcatcattg ctcaaatggt
     3121 tgccatagtt gatgtcgcct atttgcacgg tgatgctggt ttgaaaatgc cactcttggg
     3181 gtaagtctat tttgatgcga ctcatgtttg cagtcctttc ggttgcaggg tgggcgaagt
     3241 atgttaagct gtttttatgc taattgcgca aggaggtcaa tatgtatcag cacatactgg
     3301 tgccatttga tgacagtcaa gcggctttgg tggccttggt tgaggcttgt aaagtggggc
     3361 gcttaacggg cggcaccttg cacattgtgc atgtggtcga cttggcgcag ttttcttggt
     3421 cgggtgcgag ctttttgcgc agtgaggaat tggccatggc cagcgccgat actgccaaag
     3481 cggtgtttga acgtgcggag ccgattatgg cggagtatgc agatgtccaa gccgagcaca
     3541 cggtattgga ggcggtgggt gagaaagtgg catcgctgtt ggtgaacaaa atcagcgagt
     3601 ggcaatgtga cttggtggtg atgggaaccc atggattcag cggagtgaaa cacttgctga
     3661 tgggctcggt ggcgcaagat gtgatacgcc aagcaggcat tcctgtgttg attctgcgtg
     3721 tggctgcacc ttaaaatatc cattctctat gaaataagcg gcctgtaatt gttacaggcc
     3781 gcttattttg tttgtgtagt aaattctatg cgttgcttgg tatgctgttt tgaggttgtg
     3841 ccaaagcatt gattaaggct gaggcaagta tcatggcgat gcccacgtac tgtggccatc
     3901 ccaatggttc atgcaaaatc actgccgcca gcaataccgc cgtcaagggt tcaaaaatgg
     3961 tcaaaacctg tgcgcgggtg gctggcatcc attgcaaagc cttggtgtac aaagtgtaag
     4021 gcagcaaggt gccaaccact gcaatcatca aggccaccat ccaaaaattg ggcgccatat
     4081 cagtaaacat tttctgcatt gcgctgtgaa aaaaaggcat gaacaacatg gccatgccgc
     4141 tgatgaggac actgctgtac agcaaaatgg tgggaggggt gttgttcagt gcttttttgc
     4201 ccaaaacccc aaaacacgcg tagctgaagc cagacagcaa accaaaggca atgccagtcc
     4261 agtttaaggg ggcagaagta ttcaagcaca aggccacgcc cacgacggtg atcaaagtca
     4321 acatcacacc gctgaggctc atgcgctctt tcaataaaaa gtgagccagt aacatgctcc
     4381 acacgggcgc ggtatacagc agcgccaccg ataaggcagc gcctatgtgt tgtaccgcca
     4441 agaaatagca aactgtcatc aaaaacacgc cgagcagcga ttgcatgctc aataaaggca
     4501 gttgtttgcg ttgaaagaca cgcaagctgt ttaagacgaa tggactgaca aggattaaaa
     4561 ccacagaagc caaggtaatg cgcaaactgg ccacttccaa acctgaaaag cctgcttgat
     4621 tcagtttggc acccaaaagc ccaatcaagc cccagcacga ggctgccagc accacaaaca
     4681 cacaacccaa aaaataacgc tgctttgcca ttgccatcac tgtcgcagtt aaaaaccgac
     4741 actatgccac agtccatcag ctacagtcag cagggcagtg tgttgtggtg agggtgttta
     4801 tggaatgatg ccgcaagcga tgcgcgcacc acctccaccc agtgcttgag gctggtcgct
     4861 gtaattgtcg ctgcccgcat gcaccatgat ggcgcgttgg cgcacatcgc tcaagtgtag
     4921 attcggtgcc aacaccgcgg tggtggcagt gccgttggct gcaacctgta gcacaggcaa
     4981 atcaccagca tgtccatggg cttgtggtcc caagtgttta cctgtgttgt gtgggtctaa
     5041 gtggccgcca gcggcttggg ctgccgtgag ttttccctcg gcattgttgg cggtggcgca
     5101 gctgggagtg ccatgaatgt ggaaaccatg ggggccagga ggcagggcgc tgagctgagg
     5161 ttgtatcagc agtccttggg gcgtatcttt gaatgacaca tggcctatgg acaagcccac
     5221 accttgtgaa gaaacgctgt tgacttcagc ttggacactg tttgaagaag cagtgctttg
     5281 gcaagctgcc aaaatgaaac tgctgcacac aagtgcaaaa taaatcgggt atttatgaga
     5341 agacatggtg cgctcctgat tggcgaaagt agccattagt gcgcaatagg aatatttggt
     5401 tcaaatagaa attgaggtat taccgaaaga atggtcgggg cggtgggatt cgaactcacg
     5461 accctctgct cccaaagcag atgcgc
//
LOCUS       DAOMVK010000100         5461 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_4113_length_5461_cov_3.547355, whole genome shotgun sequence.
ACCESSION   DAOMVK010000100 DAOMVK010000000
VERSION     DAOMVK010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 5461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5461
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4113_length_5461_cov_3.547355"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..871)
                     /locus_tag="PK856_04725"
     CDS             complement(134..871)
                     /locus_tag="PK856_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HRL01711.1"
                     /translation="MFKAISLALALLGSTSVFAAGAAPWSYHGETAPEHWSELSPEYA
                     TCKAQNQTPINIRNAAKTNLAPLYMAYSSDAQKITNTGHTVQISYAEGSTIEVDGEKF
                     TLKQMHFHTPSENQIQGKSYPMELHLVHANADGALAVVGVMFVEGKDNADLAKLWRAM
                     PKKAGESVNLLSENTVSAYSFLPKKQEYYRFSGSLTTPPCSEGVRWLVMKTPIEASTA
                     QIKAFTDTMGTHTNRPIQALNGRVILE"
     gene            complement(1258..1332)
                     /locus_tag="PK856_04730"
     tRNA            complement(1258..1332)
                     /locus_tag="PK856_04730"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1297..1299),aa:Glu,seq:ttc)
     gene            complement(1343..1419)
                     /locus_tag="PK856_04735"
     tRNA            complement(1343..1419)
                     /locus_tag="PK856_04735"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1383..1385),aa:Arg,seq:acg)
     gene            complement(1476..1550)
                     /locus_tag="PK856_04740"
     tRNA            complement(1476..1550)
                     /locus_tag="PK856_04740"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1515..1517),aa:Glu,seq:ttc)
     gene            complement(1570..1646)
                     /locus_tag="PK856_04745"
     tRNA            complement(1570..1646)
                     /locus_tag="PK856_04745"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1610..1612),aa:Arg,seq:acg)
     gene            complement(1742..1816)
                     /locus_tag="PK856_04750"
     tRNA            complement(1742..1816)
                     /locus_tag="PK856_04750"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1781..1783),aa:Glu,seq:ttc)
     gene            complement(1827..1903)
                     /locus_tag="PK856_04755"
     tRNA            complement(1827..1903)
                     /locus_tag="PK856_04755"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1867..1869),aa:Arg,seq:acg)
     gene            complement(2231..3103)
                     /locus_tag="PK856_04760"
     CDS             complement(2231..3103)
                     /locus_tag="PK856_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRL01712.1"
                     /translation="MSDLDKAGALLVVILWGLNFYFIHLGLLDTSPYLLGFLRFICVI
                     FPAIFFIKKPDVPWKWLILYGLFISFGQFSFLFGAIAAGMPTGLAAIILQSQAFFTIV
                     IAALMMGERVKTYQITAMVIAALGLILIAYGQYQGQLPVLGVLLILLAALSWAIGNIT
                     VKHIGAVNPMALVIWGNVICPLPFLIAALSIDGWESTLTQLQHWSWGRVISLAYISWI
                     ASIVGYGLWGKLLSKHSAASVAPLSLFVPAVALLVAYFVLHEPLNRWHVFGIVVLLIG
                     LMVHLLLPSLRKTA"
     gene            complement(3100..>3306)
                     /locus_tag="PK856_04765"
     CDS             complement(3100..>3306)
                     /locus_tag="PK856_04765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter dimerization
                     domain-containing protein"
                     /protein_id="HRL01713.1"
                     /translation="TRLSGETRIIQCHIEVQDNLPLLQAHDIAVAAEHNIAALFEDSE
                     IIIHLDPLSVVNTHTTTDTQGCLS"
     assembly_gap    3307..3319
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3320..4020)
                     /locus_tag="PK856_04770"
     CDS             complement(<3320..4020)
                     /locus_tag="PK856_04770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRL01714.1"
                     /translation="MSSRARLLKLATYSSVCVAILLIVIKAVVWFMSGSVSILASLVD
                     SLMDAGASVLNLLAVRLALQPADHEHRFGHGKAEGIAALFQAAFIMGSALFLMLNAID
                     RIRHPSELHITPVVFAVMLASLLITIALVAFQQWILKQVHSSAVAADRLHYLTDILSN
                     IAVLIALGVSLYGWHQTDAIIGIGLALWIGWSAIGIGREALNMLMDKALPDTDLAAIN
                     SAVLQTKGVLGMHDLR"
     gene            4111..4572
                     /locus_tag="PK856_04775"
     CDS             4111..4572
                     /locus_tag="PK856_04775"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRL01715.1"
                     /translation="MLNQFETIIEPFAALSLIQLHQIMQLRQQVFMLEQQSLYLDADN
                     NDVNATHVMVYDGAKLIAYARIVPPHLSIPHAQIGRVVVASAYREQGLGSAVLQAALE
                     TAQRQFPQLDVVIAAQTAQQAWYTGFGFVVEGEVFDDGGVEHVKMRLVHTA"
     gene            4581..4832
                     /locus_tag="PK856_04780"
     CDS             4581..4832
                     /locus_tag="PK856_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01716.1"
                     /translation="MSLELEKRLEFLEEQIEVVRMENRVLTAAIKGIVRGLPQDLAVD
                     VIESIQFTFDNTSGELNYHNHPQADVFHDVTYDFFHEKE"
     gene            complement(5084..>5461)
                     /locus_tag="PK856_04785"
     CDS             complement(5084..>5461)
                     /locus_tag="PK856_04785"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HRL01717.1"
                     /translation="KGNALLYQTDAHTLHFAKQHLWAQPLDLAVRNQLANALNQRGSA
                     WRFVPAELKSSEQELVVYIENFNGRYDGATQIDGYTAWRDHGKTTTGRNFSVTTPQQG
                     DGYNAMVESLSAGLQQVATEILP"
BASE COUNT         1440 a         1232 c         1434 g         1342 t
ORIGIN      
        1 aacattatac gctcattcgt caccgctttc atgagctagg ccaaggattg ttgatttgac
       61 tgagggtaaa cacaaaaaat gcggcctgta atcaggccgc acatttcaaa tcacatcggt
      121 ttatgattgg attttattct aggatcacac gaccattcaa ggcttgaata gggcgattgg
      181 tgtgggtgcc catggtatcg gtaaatgctt tgatttgcgc ggttgaggct tcgatagggg
      241 ttttcatcac cagccagcgc acaccttcag agcaaggtgg tgtggtcaat gagccgctga
      301 aacgatagta ttcttgtttc ttaggcaaga agctgtaagc agaaacagtg ttttcactga
      361 gtaaattgac gctttcaccc gctttttttg gcatggcgcg ccacaattta gccaagtcag
      421 cgttgtcttt tccttccaca aacatcacgc caaccactgc tagtgcacca tcggcattgg
      481 catgcaccaa atgcagttcc attggatagc tcttgccttg gatttggttt tcacttgggg
      541 tatggaagtg catttgtttt aatgtgaact tttcaccatc cacttctatg gtgctgcctt
      601 cagcgtagct gatttggacg gtgtggcctg tgttggtgat tttttgggca tcgctggagt
      661 aagccatgta aagcggggcc aaattggttt tggcggcatt acggatgttg ataggtgttt
      721 ggttttgcgc tttacaagtg gcgtattctg gcgataattc tgaccagtgt tcaggcgcgg
      781 tttcgccatg ataagaccaa ggtgctgccc ctgctgcgaa tacagaggtg ctgcccaaca
      841 aagccagtgc aaggctgatc gctttaaaca taattgctac tcctgaatgg gattgctagt
      901 gagtaagaga ggtgatgcgc tgccaccgtg taagagcaag tactcttact tgtaaagatt
      961 aaatcataat agagtaagaa gtgtaaaggt ggcgcgggcc aaggaatgta gcttaaatgg
     1021 gaattggctt atagatgatg ggtttaaaat tgggcttttg taggaaatac tcaacacttc
     1081 aggtttgaaa tctgcttttg caatgaatat tgcccatgga atgctggtag agagcttgtt
     1141 attcgcgaaa caaatacatg agagtacggt aatttttcat gaggcttgta agcgaattga
     1201 aaaaccagtg aagcctttat tgagagacag atttgaatca gaatgtacgg gaaatcttgg
     1261 tacgcccacg gggaatcgaa ccccggttgc cgccgtgaaa gggcagtgtc ctaaccgcta
     1321 gacgatgagc gcgtatcttc agtggtgcac ccggagcgat tcgaacgccc gaccctctgg
     1381 ttcgtagcca gatactctat ccaactgagc tacgggtgca tttcttgcat gtccgcgact
     1441 gtaacagcaa atgtagattc gtgagaatca ttgtgtggta cgcccacggg gaatcgaacc
     1501 ccggttgccg ccgtgaaagg gcagtgtcct aaccgctaga cgatgagcgc atatagacga
     1561 acagtttatt ggtgcacccg gagcgattcg aacgcccgac cctttggttc gtagccaaat
     1621 actctatcca actgagctac gggtgcggta tctatcacta aacaaaactg ggtgatacaa
     1681 actgccagaa ctaaaacttg cttcggttaa aaaaccagcc taaggctgga attttgaaaa
     1741 ctggtacgcc cacggggaat cgaaccccgg ttgccgccgt gaaagggcag tgtcctaacc
     1801 gctagacgat gagcgcgtat cttcagtggt gcacccggag cgattcgaac gcccgaccct
     1861 ctggttcgta gccagatact ctatccaact gagctacggg tgcggtgtta atcacttgct
     1921 gtgcttgtga ttgcttaact gcgacaacgg aggcaaatta tagggatggg tcgcggtctt
     1981 ggcaagcgct ttttgcaatt atttttggag tgattcatca ctgtgcttgc gtttagacgg
     2041 catttgctga ggcgggtgtg caacaagtgt ttgagcgcgt taggtattag tttgggatgt
     2101 ggatggaatg aggaaaaggg aatcgcatgg gatatttttc tcaaaccaac ttacaggccg
     2161 cttaaaatgc cacttctgag caagaaaggc ggcctgtaaa agggttggaa aggtaaataa
     2221 cagtccgtaa tcaggcagtt ttacgcaggg acgggagcaa taaatgcacc atcaatccaa
     2281 tcagcaaaac cacgatgccg aagacatgcc aacggttgag tggctcgtgc agtacaaaat
     2341 aagccaccag caatgccaca gcagggacaa acaacgacag tggcgcaaca ctggcagcgc
     2401 tgtgtttgct taataatttt ccccacaaac catagccgac gatgctggct atccaactga
     2461 tgtaggccaa ggatatgaca cggccccagc tccagtgttg caattgggtg agggtggatt
     2521 cccagccatc aatgctcaaa gcggcgatca aaaaaggcaa tgggcaaatc acattgcccc
     2581 atatcaccaa ggccataggg ttgacggcac cgatgtgttt gactgtgatg ttgcctatgg
     2641 cccaagacaa ggcggccagt aaaatcaata acacgcccaa tactggcaac tgaccttggt
     2701 attgaccata ggcaatgagg atcagcccca aagccgcaat caccatggcg gtgatttgat
     2761 aggtttttac ccgctcgccc atcatcaacg ctgcaatcac aatggtgaaa aaggcttgtg
     2821 attgcaaaat gatggcggca aggcctgttg gcatgccggc ggcaatcgcg ccaaagagaa
     2881 aactgaattg gccaaaactg ataaacaagc catacaaaat cagccatttc cacggcacat
     2941 caggcttttt aatgaagaag atggcaggga aaatcacgca aataaagcgc agaaaaccca
     3001 ataaataagg ggaggtatcg agtaaaccca agtggatgaa atagaaattc aatccccata
     3061 aaatgacgac gagtaatgca cctgctttgt ccaaatcgct catgaaaggc aaccttgtgt
     3121 gtctgtggta gtgtgtgtgt tgaccacgct tagtgggtca aggtggatga tgatttcgct
     3181 gtcttcaaac aaggcagcga tgttgtgttc tgccgccacg gcaatgtcat gtgcttgcag
     3241 taagggcaga ttgtcttgta cttcaatgtg acattggatg atgcgggttt caccagacaa
     3301 gcgcgtnnnn nnnnnnnnnc gcaaatcgtg catgcccagc acacctttcg tttgcaggac
     3361 ggcactgttg atggctgcca aatcagtgtc tggcaaggct ttgtccatca gcatattgag
     3421 ggcttcacgg cctatgccta tggcgctcca gcctatccac agcgccaagc ctatgccgat
     3481 gatggcgtcg gtttggtgcc aaccatacag gctcacaccc aaagcaatga gtaccgcaat
     3541 gttgctgagg atgtcggtga ggtagtgcaa gcggtctgcg gccaccgcac tggaatgcac
     3601 ttgtttcaat atccattgct gaaatgccac caaggcaatg gtgatgagga gtgaggccag
     3661 catcactgca aacaccacag gggtgatgtg caattcactg gggtggcgga tgcggtctat
     3721 ggcattgagc attaaaaaca gtgccgaacc catgatgaag gcggcctgaa acaaggcggc
     3781 gatgccctcg gctttgccgt gtccgaaacg gtgttcatgg tcggcaggct gtaaggccaa
     3841 gcgcacggcc aataaattga gcacactggc acccgcatcc atcaacgaat ccaccaagct
     3901 ggccaaaata ctcactgagc cactcatgaa ccacaccacg gctttgatga caatcaacaa
     3961 gatggccacg cagacggagc tgtaagtggc gagtttgagt aagcgggcgc gagacgacat
     4021 aatttctttc tgtaaggcca tagcacagat tgattagacc tgaatggatt ttgcagctaa
     4081 ggtgggcgtt ttcgttcata gaaggtcggt atgttgaatc aatttgaaac catcatcgag
     4141 ccctttgcag ccctgagctt aatccaattg catcaaatca tgcaactgcg ccaacaggtg
     4201 tttatgttag agcagcagag tttgtatttg gatgcggaca acaacgatgt caacgccacc
     4261 catgtgatgg tgtatgacgg tgccaaattg atcgcctacg cgcgcatcgt gccgccgcac
     4321 ttgagcattc cccatgcgca aatcggccgt gtggtggtgg cgagtgctta tcgtgagcaa
     4381 ggtttgggca gtgccgtgtt acaggccgca ttggaaaccg cgcagcgcca attcccgcaa
     4441 ttggatgtgg tcatcgctgc ccaaaccgca caacaggctt ggtacacagg attcggtttt
     4501 gtggttgagg gcgaggtatt tgacgatggt ggtgtagaac acgttaaaat gcgtctggta
     4561 cacacagctt aggaagtatc atgtcattag aattagaaaa gcgtttggaa tttttagaag
     4621 aacaaattga agtggtgcgc atggaaaacc gtgtgctcac cgctgcgatt aaaggcatag
     4681 tccgtggctt accacaagac ttggccgtgg atgtgattga atccatccaa ttcacctttg
     4741 acaacaccag tggcgaattg aattaccaca atcacccaca agccgatgtg ttccacgatg
     4801 tgacttacga tttcttccac gaaaaagaat aagcctgaca cagcgttaaa agcggcctgt
     4861 aaatgtttaa agtgtttaca ggccgcttgt ttgtgtgggg tgacacttga acgagcgagt
     4921 gtgtcaaaat tgtttgcgtt atattagaca agaatcagca tttgcaatgg attaatggga
     4981 attgctgttt aagctagtat ggcaaacaca ccgatgctat aaaaaagcgg cctgcaaact
     5041 gggaagtggt ttacaggccg cttattgatg tggtcttaag actttaaggc agtatttcgg
     5101 tggccacttg ctgcaaaccc gcgctcaaag actctaccat ggcattgtag ccatcgcctt
     5161 gctgtggcgt ggtcacgctg aaattgcggc cagtcgtggt tttaccatgg tcacgccaag
     5221 cagtataacc atcgatttgg gtggcaccat cgtagcggcc attaaaattc tcgatgtaaa
     5281 ccaccaattc ttgctccgag ctttttaatt cggcaggcac aaaacgccat gcactgccgc
     5341 gttgattcag tgcattggcc aattggttgc gcacggccaa atccaaaggc tgtgcccaca
     5401 aatgctgttt ggcaaaatgc aaggtgtgcg catcggtttg gtagagcaag gcattgcctt
     5461 t
//
LOCUS       DAOMVK010000101         5442 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_4130_length_5442_cov_2.981251, whole genome shotgun sequence.
ACCESSION   DAOMVK010000101 DAOMVK010000000
VERSION     DAOMVK010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4130_length_5442_cov_2.981251"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /gene="murD"
                     /locus_tag="PK856_04790"
     CDS             <1..896
                     /gene="murD"
                     /locus_tag="PK856_04790"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRL01718.1"
                     /translation="WMQNQGEADVWVLELSSFQLDTTYSLQAAAATVLNISEDHLDRY
                     DDLLDYAHSKSHIFSGNGVQVLNADDALCVAMHRQGRPVKWFSLKQQQDFWVNLQAAS
                     LPLMAGDEVLLNVGDLSLQGLHNAANALVAVALCEAIGLQKADLLPHLKTFKGLPHRV
                     EKIGAYQNVDFIDDSKGTNVGATCAALYGLQQPIVLIAGGLGKGQDFAPLQAALQNKA
                     RAVMLIGRDADQIEAALQGLDAPVIRCESLAEATQKGFELAQAGDAVLLSPACASMDM
                     FRDYAHRSEVFIHAYQELAHA"
     gene            893..2050
                     /gene="ftsW"
                     /locus_tag="PK856_04795"
     CDS             893..2050
                     /gene="ftsW"
                     /locus_tag="PK856_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HRL01719.1"
                     /translation="MNSRASSKLLDRTLLKNGVTFDWSLLWVIVLMTAFSVTMIFSAS
                     IEHAEFNYQDRYYFVERQLIFLSIGYAVLLAMLKTPTRTLHKFMWLILGISFLLLIGV
                     LFGREINGAKRWINIGPFNIQPTEIFKIAIILYLASFLTRRVDILTKFSKVWFVGIPI
                     GLGLGLIILEPDFGSFVVVSVIAVGLLFIGGLPMGWFMAIVALGAGAMVTLIVSSPYR
                     MHRITGFLNPWEDPFGKGYQLTHALMAFARGEWLGVGLGGSVEKRFYLPEAHTDFIAA
                     VIGEELGFAGLILLIGCYMWLVWRSFSIGKQARDLEQYFSAYTAKGIGIWIGIQSFFN
                     LGVNLGLLPTKGLTLPLMSYGGSAVIVMLMCLGMLLRIDWENRRIMRGYKV"
     gene            2047..3129
                     /gene="murG"
                     /locus_tag="PK856_04800"
     CDS             2047..3129
                     /gene="murG"
                     /locus_tag="PK856_04800"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HRL01720.1"
                     /translation="MSKATVMVMAGGTGGHIFPALAVARELQAAGYEVVWLGADGGME
                     TRIVPPTGIQLETIAIKGVRGNGLKRKLMLPLTLASNIHAVRRIINAHHVKALIGFGG
                     FVTFPGGMGGKLSGLPLTIHEQNAIAGLSNKVLARYAKRVLYAFPKVFPNADGLVGNP
                     VRADIVAMPAPAERFAGRTGRLRLLVVGGSLGAKVFNDVVPQALALLPEEQRPVVTHQ
                     SGRNKLQDLQQSYASLGLEAQCVEFIDDMVAAYAEADVVVCRAGALTVAELAAAGLGA
                     IFVPYPHAVDDHQTHNAKVLVDAEAAMSVPQGEFTAQHLADLLQKLDRSNCLQWAEQA
                     RKQALPDAAKLTAQAVISTMAADKKD"
     gene            3132..4529
                     /gene="murC"
                     /locus_tag="PK856_04805"
     CDS             3132..4529
                     /gene="murC"
                     /locus_tag="PK856_04805"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HRL01721.1"
                     /translation="MMKNLVKRVHFVGIGGTGMCGIAEVLHNLGYQVSGSDQSQNTTT
                     QQLKNMGITVFHGHHAENVENVDVVVTSTAINRDNPEVQAALTEHIPVIPRAMMLAEL
                     MRFRQGIAIAGTHGKTTTTSLTASILGAADLDPTFVIGGKLNAAGANAQLGKGDYIVA
                     EADESDASFLYLSPVMAVITNIDTDHMDTYDHSVDKLHQAFIDFVHRLPFYGRAFLCR
                     ESEHVRAILPKINKPFSTYGFTAESDIYASDIVAEGMQMRFTVHLKDMSVRPFEVVLN
                     TPGQHNVLNALASIGIALACGVNVEAIQAGLLAFGGVGRRFQCYEGLKLPQGGSVNLI
                     DDYGHHPVEIAATLAAVRGAYPEQRLVLAFQPHRYTRTRDLFEDFVSVLNQADALLLT
                     EVYPAGETPIVAADSKALARAIRVHGKIEPIYVETVAELPLSLVHVLQDGDILLNMGA
                     GNISTLPKLLKNQLA"
     gene            4540..5441
                     /locus_tag="PK856_04810"
                     /pseudo
     CDS             4540..5441
                     /locus_tag="PK856_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
BASE COUNT         1254 a         1100 c         1548 g         1540 t
ORIGIN      
        1 cgtggatgca aaaccaaggt gaggccgatg tgtgggtgtt ggaattgtcc agctttcaac
       61 tcgacaccac ttacagctta caggccgctg ctgcgacggt attgaacatt tcagaagacc
      121 atttagaccg ctacgatgat ttattggatt atgcgcacag caaatcccac atcttcagtg
      181 gcaatggtgt acaggtattg aatgcagacg atgctttgtg cgtggccatg caccgtcaag
      241 gtcggcctgt aaaatggttt tcactgaagc aacaacaaga cttttgggtg aatttacagg
      301 ccgcttcttt gcctttgatg gcaggggatg aggtcttgtt gaatgtgggt gatttgagtt
      361 tgcaaggctt gcacaatgcg gccaatgctt tggtggcggt ggctttgtgt gaggccatag
      421 gtttgcaaaa agccgatttg ttgccacatt tgaaaacctt caaaggcttg ccgcaccgcg
      481 ttgaaaaaat cggtgcttac caaaacgtgg atttcatcga cgacagcaaa ggcaccaatg
      541 tgggtgcgac ttgtgcggct ttgtatggtt tgcaacagcc catcgtgttg attgcgggtg
      601 gcttgggcaa agggcaagac tttgcgcctt tacaggccgc attgcagaat aaagcccgcg
      661 cggtgatgtt gataggccgt gatgccgatc aaattgaagc ggcattgcaa ggcttggatg
      721 cacccgtgat tcgatgtgaa agtttggctg aagccacgca aaaaggcttt gaattggcgc
      781 aagcagggga tgcagtcttg ttaagtccag cctgtgccag catggacatg ttccgtgatt
      841 atgcccaccg ttcagaggtg ttcatccatg cttaccaaga attggcccac gcatgaatag
      901 ccgcgcaagc tccaaactcc ttgaccgcac cttgttaaaa aatggggtga cgtttgattg
      961 gtcgttgttg tgggtcattg tgttgatgac ggcgttcagc gtgacaatga ttttttcggc
     1021 gtccattgaa catgctgaat tcaattacca agaccgctat tactttgtgg aacgccagct
     1081 gatttttttg agcataggct atgcggtttt gttggcaatg ttgaaaacgc ccacgcggac
     1141 tttgcacaaa ttcatgtggt tgattttggg tatcagtttc ctattactca tcggggtgtt
     1201 gtttggtcgt gaaatcaatg gtgccaagcg ttggattaac attgggccat ttaacatcca
     1261 acccacggaa atttttaaga ttgccatcat tctgtatttg gccagcttct tgacccgtcg
     1321 ggtagacatc ctgaccaagt tcagtaaagt atggtttgtc gggattccca ttggcttggg
     1381 gctgggtttg attattttgg aaccagactt tggttcattt gtggtggtca gtgtgattgc
     1441 ggtgggcttg ctgtttattg gtggtttgcc catgggatgg tttatggcga ttgtggcttt
     1501 gggcgcaggc gccatggtga ctttgattgt cagctcacct tatcgcatgc accgcatcac
     1561 aggctttcta aacccatggg aagatccgtt tggtaagggc taccaattga cccatgcctt
     1621 gatggctttt gctcgaggcg aatggctggg tgtgggcttg ggtggcagcg tggaaaagcg
     1681 cttttacttg cctgaggcgc atactgactt tattgcggcc gtgatcggtg aagagctggg
     1741 ttttgcaggc ttgattttgt tgataggctg ttacatgtgg ttggtatggc gttcgttttc
     1801 cataggcaaa caagcgcggg atttggaaca gtatttttct gcttacacag ccaaaggcat
     1861 aggcatatgg ataggcatcc aaagcttttt taacttgggt gtgaatctgg gtttgttgcc
     1921 caccaaaggc ttgaccttgc cactgatgtc ttatggtggt tctgcggtga ttgtgatgtt
     1981 gatgtgtttg ggtatgttgc tgcgcatcga ttgggaaaat cgacgcatca tgcgagggta
     2041 taaagtatga gtaaagccac agtgatggtg atggcaggtg gcacaggtgg gcatattttc
     2101 cctgcgttgg ctgttgcacg agaattacag gccgctggtt acgaggtcgt gtggctgggt
     2161 gctgacggtg gtatggaaac ccgtatcgtg ccaccgacag gcatacagtt ggaaaccatt
     2221 gccattaaag gcgtgcgtgg caatggattg aagcgtaagt tgatgttgcc tttgactttg
     2281 gcgagcaata ttcatgcagt gcgacgcatc attaacgccc atcatgtcaa agccttgatt
     2341 ggttttggcg gctttgtcac ttttccaggt ggcatgggcg gtaaattgtc gggtttgcct
     2401 ttgaccatac acgagcaaaa tgccattgca ggtttgtcaa ataaggtttt ggcacgttat
     2461 gcaaaacgtg tattgtatgc gtttccaaaa gtgttcccca atgccgatgg cttggtaggc
     2521 aatcctgttc gagccgatat cgtggccatg ccagcacctg ctgaacgttt tgcagggcgt
     2581 acaggccgct tgcgtttgtt ggtagtgggt ggcagtttgg gtgcaaaagt gtttaatgat
     2641 gtggtgccac aggctttggc tttgttgcct gaagagcagc gccctgtggt gacgcatcag
     2701 tcaggccgca ataaactaca agatttgcag caaagctatg ccagcttagg gctggaagcg
     2761 caatgtgtgg aattcatcga tgacatggtt gcagcctatg ctgaggccga tgtggtggtg
     2821 tgtcgtgcgg gtgcgttgac ggtggcggaa ttggctgcgg caggattggg agcgattttt
     2881 gtgccttatc cacatgcagt agacgaccac cagacccaca acgcaaaagt gttggtggat
     2941 gcggaagcgg caatgagtgt gccacaaggc gaattcaccg cacagcactt ggctgattta
     3001 ttgcaaaaat tagaccgatc aaattgttta caatgggcgg agcaggcgcg caaacaggct
     3061 ttgccagatg ccgcgaaact cacagcgcaa gccgttattt caaccatggc ggcggataag
     3121 aaggattaat aataatgaaa aatttggtca aacgtgttca ttttgtcggc ataggcggca
     3181 caggtatgtg cggcattgcg gaagtgttgc ataacttggg ctaccaagta tctggttctg
     3241 atcagtcaca aaacaccact acccagcagt taaaaaacat gggcattact gtgttccatg
     3301 gtcaccacgc agagaatgtt gaaaatgttg atgtggtggt gacttctact gccattaatc
     3361 gcgacaaccc tgaagtacag gccgctttaa ctgagcacat tcctgtgata ccacgcgcca
     3421 tgatgttggc tgagttgatg cgctttcgcc aaggcatcgc cattgcgggt acacatggca
     3481 aaactacgac caccagtttg accgcttcca ttttgggcgc agctgatttg gatcccacct
     3541 ttgtcattgg cggcaaattg aatgcggcag gtgccaatgc acaattgggc aagggtgact
     3601 atattgtggc tgaagcagat gagtcggacg cttcattctt gtatttgtcg cccgtgatgg
     3661 cggtgattac caatattgat accgatcaca tggatacata cgatcacagt gtcgataaat
     3721 tgcatcaggc attcattgat tttgtgcacc gtttgccatt ttatggtcgc gctttcttgt
     3781 gtcgtgaaag cgaacatgtg cgtgcgattt tgcctaaaat caacaagccg ttctcaacgt
     3841 atggttttac agctgaatcg gatatttacg cgtcagacat cgttgctgag ggcatgcaaa
     3901 tgcgtttcac agtgcatctg aaagacatgt cggtgcgccc atttgaagtg gtgttaaata
     3961 ccccaggaca acacaatgtc ttgaatgctt tggccagtat tggcattgcc ttggcttgtg
     4021 gtgtgaacgt tgaggcaata caggccggat tgttagcgtt cggtggtgtg ggcagacgtt
     4081 ttcagtgcta tgaagggcta aaactgccac aaggcggcag tgtgaacctg atagatgact
     4141 atggtcacca cccagtagaa atcgcggcaa ccttagcggc agtgaggggg gcttatcctg
     4201 agcagcgctt ggtattggcg ttccaaccgc accgttatac ccgtacccga gacttgtttg
     4261 aagattttgt ttcggtgtta aatcaagcag atgccttgtt gctgacagaa gtttatcctg
     4321 caggtgaaac ccctattgtg gctgcggaca gcaaagcttt ggcgcgagcc atacgggtgc
     4381 atggtaagat tgaaccgatt tatgttgaaa ctgtcgcgga attgccgctc agtttggtac
     4441 atgttttgca ggatggcgac atattgttaa acatgggtgc tggcaatatc agcacactgc
     4501 ccaagctttt gaaaaaccag ttggcataaa ggtaggatga tgaagcattt aggtaaggtt
     4561 gctgttttgc tgggtggtga ctctagtgag cgtgaagtgt ctttgctcag tggtgcagaa
     4621 gtgcatcagg gtttgttaca ggccggtgtg gatgcgcatt tgtatgatac ccaaaaacaa
     4681 tctttgtttg atttgaagaa acaaggattt gaacgggtgt ttattgtgct acacggtggt
     4741 gcaggtgaag atggtcgtgt acaagcggtt ttagagacca tgggtttgcc atacacgggt
     4801 tgcggcatgt tggcttctgg tttgggtatg gataaattgc gttccaaaat gatttggcaa
     4861 gccgcgggct tacctgtacc tgagtttgca gtgctgcaag acgataccga ttttgatgcg
     4921 gtagaaaaaa cattgggctt gcctttgttt gtaaaacccg cagccgaagg cagcagtgtg
     4981 ggtgtggtta aagtcactga aaaaggcact ttggctgagg tttatcaaga tttgaaacaa
     5041 taccgtggtg ccatcattgc agagcggttt atcggtggtg gtgagtatac ttgtggtatc
     5101 ttaggttttg aggccttgcc taccattaaa atcatcccga aaactgagtt ttatgattac
     5161 gaagccaaat acttccgtga tgacactgag tatttgtgcc cagctgattt gactgatgct
     5221 tgatgaggca gttggctttg caggcatttg aggttatcgg tgggcgcggt tggggccgtg
     5281 tagacttctt gcgtggtgtg gatggtaaac tgtatttatt ggaaatcaac acagttcccg
     5341 gcatgacgac ccacagcttg gttcctaaag ctgctgcaca aacaggccgt tctttttcac
     5401 aattgtgttt ggaaatctta gagcatgcga ctttgggatg at
//
LOCUS       DAOMVK010000102         5439 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_4134_length_5439_cov_2.370171, whole genome shotgun sequence.
ACCESSION   DAOMVK010000102 DAOMVK010000000
VERSION     DAOMVK010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 5439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5439
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4134_length_5439_cov_2.370171"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="PK856_04815"
     CDS             <1..841
                     /locus_tag="PK856_04815"
                     /inference="COORDINATES: protein motif:HMM:NF018485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HRL01722.1"
                     /translation="LMVLAPAWALRQMPTAAKNRASALIFAGIGIGILFSAFLLPYMP
                     ALGLKLTWLILGCASVLLSILIASLLLTHPNQAKPSPTQTKSLNTSTAQPATAAAILI
                     ILAYGCFGMGYLPHALFWVDYLARDLGWDLHTVDTQWLLYGLGASIGNGLGFVLVKYC
                     GWLKANSICFILYAIAIALPLASQHTSVLALSSFVSGALVPVMVAMSSGCLLLLLGNE
                     SHQRFWGYATAFFSVCQFGGGLLMSHILTSSHHYPYLFTLGSALLLIGGLLSSRSWWQ
                     QKH"
     gene            881..1627
                     /locus_tag="PK856_04820"
     CDS             881..1627
                     /locus_tag="PK856_04820"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HRL01723.1"
                     /translation="MQQTLNTTNRQLPLQACFNSRDLGGISNQAGRNIKTQLLLRADD
                     LSSLSTADVAYLCELPLHTVIDFRSTLEQNHKPDIVPDSCQRHLHLDILSGNMEVYMQ
                     QMKDSSANARAIMFEIYSDLVLNPQSQQQYRQFFNVLQQEPQGSLLFHCSAGKDRTGI
                     ATALILEALGVERDCIMQDYLLSNPYLISKYSHLFEAYPHAYDFLTVSEDFLQHAWDL
                     IDKQHGTVERYLTEVLHIDLPLMQNLYLEA"
     gene            complement(1698..1829)
                     /locus_tag="PK856_04825"
     CDS             complement(1698..1829)
                     /locus_tag="PK856_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01724.1"
                     /translation="MYVSDVQCLQSRPAVGLQAAQVAKIREFAQSLWQLQAAEPVAA"
     regulatory      complement(2091..2189)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM-I-IV-variant riboswitch; SAM riboswitches
                     modulate the expression of downstream genes in response to
                     levels of S-adenosylmethionine; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            complement(2279..2788)
                     /locus_tag="PK856_04830"
     CDS             complement(2279..2788)
                     /locus_tag="PK856_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01725.1"
                     /translation="MPKLIHQMYMLVHAAIALLFLFAALFLVYLAAQTGLKVLDEASL
                     SSSVVHVVEALGILAAAIVSVQISQTILEEEIIRDVHISGPSRIRRFLSRFLVMLVVA
                     LTIEGLIASFKAQKEPQQLLYAAALLVATASLLVGWGVFIKLNSAAEKLEPESLEEAK
                     KEDTKVEGK"
     gene            complement(2913..4190)
                     /locus_tag="PK856_04835"
     CDS             complement(2913..4190)
                     /locus_tag="PK856_04835"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRL01726.1"
                     /translation="MPDHLIAATQLRFQGLRNVSQRDGFGAELVSVIDEDKQAQNQSN
                     FSEMNASPATVLIHFKGKDLDEVLRSHDVVIQGMDPFSHETVTVNQQTVPLAANFTGA
                     YGVWLANSGFARQSLKTLLGKDGGIEHPHVYLMQPYDPDRRIILMVHGLASSPEAWVN
                     MANDLMGDYAIRQRYQIWQVYYPTNMPIALNHVAIRRVVDDTLRHFDPTGAHPASKDM
                     VVVGHSMGGVISRMMIASDNGALDAWLKESLGTNQIPASIAELAHFQPMPQIGRVIFI
                     AAPHNGTHVAGGKVGKLVSKAVRLPFRTLRTLDQALTDAFSNNKRELQRLQQRGVLVP
                     NSIDNLDEKDPFIMASQNLKISSTIPYHSIIAQYNANKALEQSSDGVVPYSSAHLPHA
                     QSEKIITSGHSVQENPQAILEIRRILHEDMRKK"
     gene            complement(4175..4501)
                     /locus_tag="PK856_04840"
     CDS             complement(4175..4501)
                     /locus_tag="PK856_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01727.1"
                     /translation="MPQQSSTYLAAMSELWLYQAQRLQKEENQVEAYHDALMKSARYA
                     YAYLFYGATPINQRVLDNRQMQVADYYNYAVQTFVNENFNRYTNAEIAASSQHGQVHI
                     ETCPII"
     gene            complement(4534..4713)
                     /locus_tag="PK856_04845"
     CDS             complement(4534..4713)
                     /locus_tag="PK856_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01728.1"
                     /translation="MKFKNLLLCGLSLYLCSCAMVSLKPQDTREYIMSKRADVLTEKH
                     LSASTMEVGVSVRNR"
     gene            4871..5332
                     /locus_tag="PK856_04850"
     CDS             4871..5332
                     /locus_tag="PK856_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01729.1"
                     /translation="MSHTDLAIRAQLQALELQLFRLSVTADASRKLPIKIPLKAMKTT
                     AQALAKVRYLRFSRSKGYRIGLIPLEPASFAILQTRIAPELPALLETFGVQVLYCNVI
                     SATKQQCWLNTVAMHVLQTQTDLQAALNTAQIEWQIHTCEQGLGLLAGFRY"
BASE COUNT         1363 a         1421 c         1193 g         1462 t
ORIGIN      
        1 gttaatggtt ttagcacccg cttgggcttt gcgacaaatg cccaccgcag ccaaaaacag
       61 ggcttcagca ttgatttttg cgggcatagg cataggcatt ttgttttctg cctttctctt
      121 gccctacatg cccgctttgg gtttgaaact cacgtggttg attttgggct gtgccagcgt
      181 attgctcagc atcctcatcg ccagtttgct tctcacccac cccaatcaag caaagcctag
      241 tcctacccaa actaagagct taaacacctc tactgcccaa cccgcgactg cagctgcgat
      301 actgattatt ttggcctatg gctgttttgg tatgggttat ttgcctcatg ccttgttttg
      361 ggtagactat ctggcgcgtg atttgggctg ggatttacac actgttgata cacagtggtt
      421 gctgtatggc ttgggcgcat ctatcggtaa tggtttgggc tttgtactgg tgaaatactg
      481 cggatggctg aaagccaaca gcatttgctt tatactctat gccatcgcaa tagcgttgcc
      541 acttgcaagc caacacactt ctgtcttggc cttatcatct ttcgtcagtg gcgctttggt
      601 gcctgtgatg gtggccatga gttcaggttg cttattattg ctgctgggca atgaatcaca
      661 ccaacgcttt tggggctatg ccacggcgtt tttcagcgtg tgccagtttg gcggtgggct
      721 gttaatgagc cacatcttaa ccagcagcca ccactatccc tatctgttca cattgggttc
      781 cgccttgctg ctgataggtg gcttattgag cagccgcagt tggtggcaac aaaaacactg
      841 acacaagcgg cctgtaaacc gtttaacctt tggagtgtgc atgcaacaaa ccctcaacac
      901 caccaacaga cagctgccct tacaagcgtg ttttaacagc cgggatttgg gtggcatcag
      961 caatcaagca ggccgcaaca tcaaaaccca attgctgctg cgtgccgacg acttaagcag
     1021 cctcagcaca gccgatgtgg cttatttatg tgagcttcca ttgcacaccg ttattgattt
     1081 tcgctccact ctggaacaaa accacaagcc cgacatcgtg cctgattcat gccagcgcca
     1141 cctgcatttg gatattttaa gtggcaatat ggaagtgtat atgcagcaaa tgaaagacag
     1201 ctctgccaat gcgcgtgcca tcatgtttga gatttatagt gacttggtat taaatcccca
     1261 aagccagcaa caataccgcc aattttttaa tgtcttgcag caagagcctc aaggcagctt
     1321 gctgtttcat tgcagtgcag gcaaagaccg aacgggtatt gcaacagctt tgatattgga
     1381 ggctttgggt gtggagcgtg actgcatcat gcaagactat ttgctgtcca acccttattt
     1441 aatcagcaaa tacagccatt tgtttgaagc ctatccgcat gcatatgatt ttctcaccgt
     1501 ctcagaggat tttctgcaac acgcttggga tttaatcgat aaacagcacg gcactgttga
     1561 gcgctatttg accgaggtat tgcacattga tttgcctctc atgcaaaacc tctatttaga
     1621 agcttgaatg accacataaa tacggcctgt aaaccacaca gccctaagaa acacttaggg
     1681 ctgttttgca taccagttta agcagctact ggttcagcgg cctgaagctg ccacaaagat
     1741 tgtgcaaatt cgcggatttt ggccacttga gcggcctgta aacccaccgc gggacggctc
     1801 tgtaagcatt gcacatcgct gacatacaat tgtcacatca taccaagcgt tcttcccaac
     1861 atggtactgg cccaaatgca attgttggct gggttggaca ggattcaccc accaaacccc
     1921 tacggtttct gaacctgcta agaatttaaa aacgatgtcg cattgaatgt ctgcaagata
     1981 acaaaggctt tgcatggttg tgttctctct tatttagcgt ttatacggtc gcaagtcagt
     2041 taaatcaccg gtcgaaaaaa aaccttccgc gaggaaggca acagtgttct gcatactcgc
     2101 ttggttgttg gccctgccaa cctcatctta tctccacctt gcccgagtgg caggttggca
     2161 tggcatgtcg ccaagtcggg atgaatgtgt gacatcataa tgtgccaaaa tggatttgtc
     2221 aaaatttctc gctgcaatgc aataaacaat gcataacaat catttaacac cgcatcggct
     2281 atttcccttc cactttggtg tcttcttttt tggcttcttc caaagactca ggctccaatt
     2341 tttcggcagc actgttgagc ttgatgaaca ccccccagcc caccaacaaa ctggccgttg
     2401 ccaccaacaa ggcagccgca tacaacaatt gttgcggctc tttttgtgct ttaaaactgg
     2461 caatcagccc ttctatggtc aaggccacca ccaacatcac caagaaacgc gacaagaatc
     2521 ggcggatgcg actggggcca ctgatgtgaa catcgcggat gatttcttct tccaaaatgg
     2581 tttgggagat ttgcaccgat actatggcag ccgccaatat acccaaagcc tctactacat
     2641 gcaccactga gctgctcaac gatgcttcgt ccaacacttt cagccctgtc tgagccgcca
     2701 gataaaccaa gaacaaggca gcaaacaaga acagcaaagc aattgctgcg tgtaccaaca
     2761 tgtacatttg atggatgagc ttgggcatga tttttctccg ctaatgccat ctgactttag
     2821 tgacccagat ttgttcagtg ggttgccata cacagccttt gtttgcacag ctatcaaata
     2881 agcggcctgt aaatgcttac aggccgcttg catcacttct tgcgcatgtc ttcatgcaaa
     2941 atgcggcgta tctccaatat tgcctgcgga ttctcttgca cactgtgccc cgaagtgatg
     3001 attttctccg actgagcatg cggcaaatgg gcactggaat acggcaccac gccatcactg
     3061 ctttgttcca gtgctttatt ggcattgtat tgcgcaataa tcgaatggta aggaatcgta
     3121 ctggaaattt tcagattttg gctggccatg ataaacgggt ctttttcatc caaattgtct
     3181 atgctgttgg gcaccaaaac cccacgctgc tgcaaacgct gcaattctct tttgttattg
     3241 gaaaacgcat cggttaaggc ttggtctagg gtgcgcaagg ttctgaatgg cagtctcact
     3301 gctttgctga ccaatttgcc cacttttcca cccgccacat gggtgccatt gtgtggtgcg
     3361 gcaatgaaga ttacccgtcc gatttgcggc atgggttgga aatgggccaa ttcggctatg
     3421 ctggctggaa tttgattcgt tcccaaagac tcttttaacc aagcatccaa agccccattg
     3481 tcactggcaa tcatcatgcg gctgatgaca ccgcccatgc tgtgccccac cactaccatg
     3541 tctttggaag ctggatgcgc gccagtagga tcaaaatggc gcaaagtatc atcgaccacg
     3601 cggcgtatgg ccacatgatt cagagcaatt ggcatattgg tggggtaata cacctgccaa
     3661 atttgatagc gttggcgtat cgcataatcg cccatcaaat cgttggccat gttcacccat
     3721 gcttcagggc tgctcgccaa gccatgcacc atcaaaataa tgcgccgatc aggatcataa
     3781 ggctgcatca aatacacatg cggatgctct atcccgccat ctttccccaa tagtgtcttc
     3841 aatgactgtc tggcaaaacc agagttggcc aaccacaccc cataagcgcc cgtaaaattg
     3901 gctgccaaag gcacggtttg ttgattgaca gtaaccgttt catggctaaa cggatccatg
     3961 ccttgtatca ccacatcatg gctgcgcaat acctcatcca aatccttgcc tttaaagtgg
     4021 attaacactg tggcaggcga ggcattcatt tcgctgaaat tgctttgatt ttgtgcttgc
     4081 ttgtcttcat caatcacaga aaccaattcc gccccaaagc catcgcgttg cgacacattg
     4141 cgcaaacctt gaaagcgcaa ttgggtagcg gcaatcaaat gatcgggcaa gtctcaatgt
     4201 gtacctgtcc atgttggctg ctggctgcaa tttccgcatt ggtataccga ttaaaattct
     4261 cgttcacaaa cgtttgtacc gcgtaattgt aataatctgc cacctgcatt tggcgattgt
     4321 ccaacacccg ctggttaatc ggcgtagcac cataaaacaa atacgcatac gcatatcggg
     4381 cgctcttcat caaagcatcg tggtatgcct caacttgatt ttcctctttt tgtaatctct
     4441 gagcttggta cagccacaat tcagacattg ccgccaaata cgtcgatgat tgttgtggca
     4501 aacccacttt ctcaatcgtc tgtatgcaat cggctatctg tttctcacac tcacgccgac
     4561 ttccatcgtc gaagcgctca aatgtttctc ggtcaacaca tccgcacgct tagacatgat
     4621 gtattcacgc gtgtcttgcg gcttcagaga caccattgca cagctgcaca aatacaagct
     4681 cagcccacac agcaacaggt ttttaaactt catcggcctc atccacagaa aatagcgaga
     4741 atcatgataa tgtggctaga gcctagattt caatccttgc agcgcatctt tacgcatttg
     4801 cagccacttc attgcctaag tcgccatact gagcgtttgt tatactgtgt ctttgccctg
     4861 ataaaatgcc atgagccaca ccgacttagc catccgcgcc caattacaag ccttggaatt
     4921 gcagctgttt cgcttaagcg tcaccgccga tgccagccga aaactgccca tcaaaatccc
     4981 cttaaaagcc atgaaaacca ccgcccaagc cctagccaaa gtcagatacc tgcgctttag
     5041 ccgcagcaaa ggctaccgca tcggcttgat tccactcgag cccgcatcgt ttgccatctt
     5101 acaaacgcgc atcgcccccg aattgcccgc cttgcttgaa acatttggcg tccaagtgct
     5161 gtattgcaac gtaatttctg cgaccaaaca acaatgctgg ttaaacactg tagccatgca
     5221 tgtgcttcaa acccaaaccg atttacaggc cgcattaaat actgcccaaa tcgagtggca
     5281 aatacacact tgcgagcaag ggctaggcct gttggcaggg tttcggtatt gagatggtga
     5341 gattgaaata gcaaacggcg aataataaaa gcggcctgta aatgtttaca ggccgctttt
     5401 tcaatatttt ttaatgctga ggatttctaa atgggttag
//
LOCUS       DAOMVK010000103         5401 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_4173_length_5401_cov_3.127011, whole genome shotgun sequence.
ACCESSION   DAOMVK010000103 DAOMVK010000000
VERSION     DAOMVK010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 5401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5401
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4173_length_5401_cov_3.127011"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..114
                     /locus_tag="PK856_04855"
     CDS             <1..114
                     /locus_tag="PK856_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003794566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HRL01730.1"
                     /translation="RTLFGSRQVGDAINIEIDAQTQAIVDTVTRVLAQQKQ"
     gene            132..1238
                     /gene="ribBA"
                     /locus_tag="PK856_04860"
     CDS             132..1238
                     /gene="ribBA"
                     /locus_tag="PK856_04860"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003772334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HRL01731.1"
                     /translation="MISTPAEIIADIKAGKIVILTDAEDRENEGDLVMAAQFVTPEAI
                     NFMIKHARGLVCLPMADELIDRLGLPMMTQNNGAQYGTNFTISIEAREGISTGISAAD
                     RAHTIQTAVASNSVASDIVQPGHIFPLRAKKGGVLVRAGHTEASVDLAQLAGLTPAAV
                     ICEIINDDGTMARMPELELFAEEHGIKMGTIADLIEYRSKTESLVEDMGCHTVHTPWG
                     DFTQHIYVDRLQGETHLALVKGDISPDSEVWVRVHEPFSAMDFISPNPSHSWTLPQTL
                     EKLQNVDAGVALLLHRPETGSELLDRALPQGSNQRSTWDSKTYGIGAQILNELNIKKM
                     RVMGKPYAFSVLNGFGLEITGFAMPNEDNSDFSV"
     gene            complement(1322..3145)
                     /locus_tag="PK856_04865"
     CDS             complement(1322..3145)
                     /locus_tag="PK856_04865"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019390219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HRL01732.1"
                     /translation="MGQQTVPEKLAALRALMRDNKTDAYVVMSADPHMSEYLPVYWQV
                     RLWLTGFTGSVGTLVISQDFAGLWVDGRYWVQAEKELSGTGFVLQKLTQDEHSSHVAW
                     LAKNLPQNAVIGVDGRALSIAQYQSLSQALARKNVRIDTEQDLISPIWQNRPDLPMKP
                     VHSMVNGLNSLSRAEKIHAIREQLQERGLNHHFVSSVDDIAWILNCRGSDVECNPVFL
                     AHLYIGLEHCVLFVQAAKIDADLQQALAADGISLQDYADSGVYLSQLNGGNILMDAAK
                     VSVAHLLALPTESNQGSVPVQVTQDINPSSLLKACKSDSEIAHVKNAMRKDGVALAHF
                     FAWLEQALADNTAITELTIAEKLLEYRSQQAGFIGESFATIAGFNENGALPHYHATEQ
                     SFSKIEGNGLLLIDSGGQYEDGTTDITRMVAIGKLTAAQKRDCTLVLKAHIALAQAVF
                     PEGIAAPLLDALARQALWQQQLDYRHGTGHGVGFTLNVHEGPQILSYYAPCSAYNKMR
                     AGMITSNEPGLYHEGHYGIRIENLVVNRLLPQTGAYGDFLAFENLTQCPIAIQCLDLE
                     MLTVNEKDWLNTYHEQVREALAGDLSGAALDWLLRQTQAVA"
     gene            complement(3392..>3655)
                     /locus_tag="PK856_04870"
     CDS             complement(3392..>3655)
                     /locus_tag="PK856_04870"
                     /inference="COORDINATES: protein motif:HMM:NF013487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRL01733.1"
                     /translation="KEAVASLDTHANQGWEYFDAATKEVKSEAQPAVWEQNDKFQQEI
                     KTFQASVAELKTASAQGGLDAIKAPLAKVGESCKSCHTTFKAD"
     assembly_gap    3656..3665
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3666..3964)
                     /locus_tag="PK856_04875"
     CDS             complement(<3666..3964)
                     /locus_tag="PK856_04875"
                     /inference="COORDINATES: protein motif:HMM:NF013487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01734.1"
                     /translation="MKKISMTLCSLMVVLALGACSGGNDAAKTETAKPDASTQTNTSA
                     ASASTGSATLSPEEQVEAREKNMKAFGKASKQLRAMVEGKEGFDDAKNDLHHKFD"
     gene            complement(4294..>5401)
                     /locus_tag="PK856_04880"
     CDS             complement(4294..>5401)
                     /locus_tag="PK856_04880"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HRL01735.1"
                     /translation="HTQAMYMTSSFTYPTAEDASRLFVGEQAGYTYSRTSNPTIAAFE
                     ERMAQLEGAERGLATSSGMAAIHATLISLLQQGDHLLSSHSLFGSTSSVVNNMLPRYG
                     IEVTCVSPVDLAAWEAAIQPNTKVLFLETPSNPLGEVADIKALAALAHKHGAVLVVDN
                     CFCTPAVQQPIKLGADISLASATKGIDGHGRAIGGIVCGSNALIEQIWNHVKTAGEII
                     SPFNAWLLSSGLETLFVRLERECSNAMALAQWLEQHPKVEKVYYGGLQSHPQHELAKQ
                     QQSMFGVVVAFEVKGARKEAWHVVDHVQLFSRTGNLGDVKSTITHPYTTTHCRVSEEG
                     KAAAGIIEGLLRLSVGLENVADLQADLEQALAGI"
BASE COUNT         1376 a         1437 c         1239 g         1339 t
ORIGIN      
        1 cgcaccttgt ttggcagccg ccaagtcggc gatgccatca atattgaaat agatgcccaa
       61 acccaagcca tcgtcgacac cgtgactcgg gtattggcac aacaaaaaca atgaaatagg
      121 aaacaaacag tatgatctct acaccagccg aaatcattgc cgacatcaaa gccggtaaaa
      181 tcgtcatctt aaccgatgcc gaagaccgcg agaacgaagg cgatttggtg atggcggcac
      241 aatttgtgac accagaagcc atcaatttca tgatcaagca cgcgcgtggc ttggtatgtc
      301 taccgatggc cgatgagctc atcgatcgct taggcttgcc gatgatgacg caaaacaatg
      361 gcgcccaata cggtaccaac ttcaccatat cgattgaagc gcgtgaaggc atctcgacag
      421 gcatttctgc cgccgaccgc gcccacacca ttcaaacggc cgtggcatca aattcagtgg
      481 ccagcgacat cgtacaaccc ggtcacatct tcccattgcg cgccaaaaaa ggcggcgtat
      541 tggtgcgtgc gggccacacc gaagccagcg tggatttggc acaattggcc ggtttaaccc
      601 ctgcggcggt gatttgtgaa atcatcaacg acgacggcac catggcgcgc atgcccgaat
      661 tggagctgtt tgccgaagaa cacggcatca aaatgggcac catcgccgat ttgattgaat
      721 accgcagcaa aaccgaaagt ctggttgaag acatgggctg ccacaccgta cacaccccat
      781 ggggcgattt cacccaacac atttacgtag accgcctgca aggtgaaacc catttggcct
      841 tggtcaaagg tgacatcagc ccagactctg aagtgtgggt gcgcgtgcat gagccattca
      901 gcgccatgga tttcatcagc cctaacccta gccatagctg gactttgccg caaaccttgg
      961 aaaaattgca aaacgtggat gcaggcgtgg ccttgttatt gcaccgccca gaaacaggct
     1021 cagaattgct cgaccgcgct ttgccacaag gcagcaacca acgcagcact tgggacagca
     1081 aaacttacgg cataggcgcg caaatcttga acgaattgaa catcaagaaa atgcgtgtga
     1141 tgggaaaacc gtatgccttc agcgtgttaa acggctttgg cttggaaatc acaggctttg
     1201 ccatgcccaa tgaagacaac agcgatttct cagtttaagc attaactact ggtttgaaac
     1261 gcaaaaagcg gcctgtaaat ctttacaggc cgcttttatc atatcaatca accatgtgaa
     1321 tttaagccac ggcttgtgtt tgacgcagca accaatccaa agctgcaccc gacaaatcgc
     1381 cagccaaagc ctcacgcact tgctcgtggt aagtgtttag ccagtctttc tcattaacgg
     1441 ttaacatttc caaatccaaa cactgaattg ctatcggaca ctgagtcagg ttttcaaatg
     1501 ccaagaagtc gccatacgca cctgtttgtg gcagcaagcg gttcaccacc aagttttcaa
     1561 tgcgaatgcc gtaatgtccc tcgtggtaca gccctggctc attggaggta atcatgcccg
     1621 cacgcatttt attgtaagca ctgcacggcg cgtaatacga cagaatttgt gggccttcgt
     1681 gcacattcaa ggtaaaaccc acgccatggc ccgtgccgtg acggtaatcc aattgttgtt
     1741 gccacaaggc ttggcgagcc aatgcatcca ataaaggtgc ggctatacct tctggaaaca
     1801 ccgcttgcgc caaagcaatg tgggctttca ataccaaggt gcaatcgcgc ttttgtgctg
     1861 cagttaactt ccctattgct accatgcgag tgatgtcggt ggtgccgtct tcgtattggc
     1921 cgcctgaatc gattaacagc aagccattgc cttcgatttt ggaaaaactc tgctcggtgg
     1981 catggtaatg gggcaaagca ccgttttcat taaagcccgc aatcgtggca aaactctcac
     2041 caatgaaccc tgcttgctgt gaacgatatt ccaacagctt ttccgctatg gtcaattcgg
     2101 taatggcggt gttgtcggcc aatgcctgct ctagccacgc aaagaaatgc gccaacgcca
     2161 cgccatcttt gcgcatggca tttttcacat gggcaatttc gctgtcgctt ttgcaggctt
     2221 tgagcaaact ggatggatta atgtcttgtg tgacttgcac aggcacactg ccttggtttg
     2281 attctgtagg caaagccaat aaatgtgcca ccgagacctt ggccgcatcc atcaagatgt
     2341 tgccaccgtt taattggctt aaatatacac cactgtcggc atagtcttgc aagctgatgc
     2401 catcggctgc caaggcttgt tgcaaatcgg catcgatttt agcggcctgt acaaacaaca
     2461 cacagtgctc caaaccgatg tacaaatgcg ccaaaaacac agggttgcat tccacatcgc
     2521 tgccgcgaca attcaaaatc cacgcgatgt cgtctaccga tgacacgaag tggtggttta
     2581 gcccacgctc ttgtaactgc tcgcgtatgg catgaatttt ttccgcacgg ctcaggctgt
     2641 taagaccatt taccatggaa tgcacaggtt tcatgggcaa atcaggacgg ttttgccaaa
     2701 tcgggctaat caaatcttgc tctgtatcga tgcgcacatt ttttcgcgcc aaagcttggc
     2761 tcaggctttg gtattgggct atcgacaaag cacgaccatc cacaccaatc actgcattct
     2821 gtggcagatt ttttgctaac caagccacat ggctggagtg ttcatcttgg gtaagttttt
     2881 gcagcacaaa acccgtgcca gacaattcct tctcagcctg cacccaatag cgaccatcca
     2941 cccacaggcc agcaaaatct tggctaatca ccaaagtgcc cactgagcct gtaaaccctg
     3001 tcagccacaa acgcacctgc caatagacag gcaggtattc agacatgtgt gggtcggcac
     3061 tcatcaccac ataagcatcg gtcttgttgt cgcgcatcaa ggcgcgcaaa gccgccaatt
     3121 tttcaggcac tgtttgttgt cccatggttt caagcttcct tttgctattg cgcgtttttg
     3181 cacactataa aatgcttaca atacccttgc aaacagggta aaagcaagac agtaattcga
     3241 ttaggtgtgt attgttggca gcatttattt tgtacacact gcttcagaat cttagccttg
     3301 cacccaatcc atatgttcat gaacacaata tgtgaaagcg gcctgtaaac cacttacagg
     3361 ccgcttgacg gtcatcgcta agttattaca atcaatctgc tttaaacgtg gtgtggcatg
     3421 atttacagct ttcacccact ttggccaatg gtgctttgat ggcatccaag ccaccttggg
     3481 ccgaagcggt tttcaactcc gccacagaag cttggaaggt tttgatttcc tgttggaatt
     3541 tgtcgttttg ctcccaaacc gcaggctgtg cttccgactt cacctcttta gtggcagcgt
     3601 caaaatactc ccaaccttgg ttggcatggg tatcaagcga ggccacggct tctttnnnnn
     3661 nnnnnttatc aaatttatgg tgcaaatcat tttttgcatc atcaaaacct tctttgccct
     3721 caaccatggc acgcaattgc ttgctggctt tgccaaaggc tttcatgttt ttctcacgcg
     3781 cttcaacctg ctcttcgggg ctcaaagtag cactgccagt gctggcagaa gcagcagagg
     3841 tattggtttg cgtgcttgca tcgggtttgg ccgtttcggt tttggccgca tcgttgccac
     3901 cagagcaggc gcccaatgcc aataccacca tcaacgaaca caaagtcatg ctgatttttt
     3961 tcatgtttca atccttacgt tatgaggaaa cttgtcacta ttgcaggtgt ttgtgctcac
     4021 cttgttattt ttcggtatgc ggttccgaca ttgctgcatc gcaaccaatg tcgcttagaa
     4081 tcgcatattt tgcggctgct tgcgagtaaa acctcaactc aaaactcgct gcatgtggtt
     4141 ttctccatac ttgaccatga ttgcatctca atggttcaca acacattatt tgcccgcaag
     4201 catgtaaaac ggtattcaaa atactgtgcc tgatgacaaa cacaaaaaaa gcaaagcacg
     4261 aagtgctttg ctttgggtat gctggcatgg gctttaaatt cctgccaaag cctgttctaa
     4321 gtcggcctgt aaatcggcaa cattctccaa acccacagac aaacgcaaca aaccctcgat
     4381 aatgcctgcc gcggctttgc cttcttcgct gacgcggcaa tgggtggtgg tgtaaggatg
     4441 ggtaatggtg gatttcacgt cgcccaaatt gcctgtgcgt gagaacaatt gcacgtggtc
     4501 aaccacatgc caagcctctt tgcgtgcacc tttcacttca aaagccacca ccacgccaaa
     4561 catggattgc tgctgcttgg ccaactcatg ttgtggatgg ctttgcaggc caccgtaata
     4621 cactttttca actttgggat gttgctccaa ccactgcgcc aaagccatgg cgttggaaca
     4681 ttcacgctcc aagcgcacaa acagggtttc caagccgctg gacaacaacc aagcattgaa
     4741 tggggaaatg atttcacccg cggttttcac atgattccaa atctgctcta tcaaggcatt
     4801 gctgccgcaa actatgccac cgatggcacg gccatgtcca tctatgcctt tggtggcaga
     4861 agccaaagaa atgtcggcac ccaatttgat tggctgctgc accgcagggg tacagaaaca
     4921 attgtcaaca accaacaccg caccatgttt gtgcgccaag gcagccaagg ctttgatatc
     4981 ggccacttcg cccaaaggat tggacggtgt ttctaagaac aacaccttgg tattgggctg
     5041 gatggctgct tcccatgccg ccaaatccac agggctgacg caggtgactt caataccata
     5101 gcgcggcagc atattattga ccaccgaact ggttgagccg aacaaactgt gactggacaa
     5161 caaatgatca ccttgttgca ataaactgat tagcgttgca tgaatggcag ccatgccact
     5221 ggacgttgcc aaaccacgct ccgccccttc caattgcgcc atgcgctctt caaacgctgc
     5281 aatggtaggg ttagaagttc ggctgtaggt ataacccgct tgctcaccca caaacagacg
     5341 cgacgcatct tctgctgttg ggtaagtaaa actgctggtc atgtacattg cttgggtgtg
     5401 t
//
LOCUS       DAOMVK010000104         5359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_4220_length_5359_cov_3.388386, whole genome shotgun sequence.
ACCESSION   DAOMVK010000104 DAOMVK010000000
VERSION     DAOMVK010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 5359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5359
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4220_length_5359_cov_3.388386"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /locus_tag="PK856_04885"
     CDS             complement(<1..1147)
                     /locus_tag="PK856_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HRL01736.1"
                     /translation="MSDNVLLQLNDEPRFDAIEVAQIEPAMKQAMTEARAAIAAVKGQ
                     ADITWDNTVTRLTDITEQVGRIWGVVAHLNSVVDTPELRAVYNGLMPEVTQFFTEISL
                     DIELYARFKAIKAAPYFAQLSAAEQKKLNNDLRDFVLSGAELSPENQEIFAGLQTESA
                     QLSAQFSQNVMDATDAFALYFADGYELAGLVQPASSPCSADAEEAVLERDSKLTGLPA
                     DALAMFAAAAEAEGKTGYKIGLQIPHYLAVMQYSDTRVLREQMYKAYVTRASELGDAK
                     LDNTANIKRILEIAAQESQLLGFANYAELSLATKMAETPDQVTAFLRDLAAKARPFAE
                     QDKAEVYAFAKKTLALDDPQPWDFTYVSEKLREAKYSFSEQEVKKYFP"
     gene            complement(1385..2092)
                     /locus_tag="PK856_04890"
     CDS             complement(1385..2092)
                     /locus_tag="PK856_04890"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HRL01737.1"
                     /translation="MAFWKTVAVLGVALSAASLVQAKDIHYNIIQLSDSANMNVPQNQ
                     LNVTLVVESNAANRNDASRDNTVKLNRVMAAIEQAGLKGQLLNRSVNEQTEYVNNKVE
                     KRGWRDYAQFSVFSEDTAKVNQLVASVQQDARLESQNYGLTTAARRQYELQLTEKAIR
                     NFQQQAQHIATVLGARNYKIVEMNVGNANVNMRPPRLYATANMAKMEMADGGAVANDM
                     AGETEISVNVSGSIQLQ"
     gene            complement(2103..2564)
                     /locus_tag="PK856_04895"
     CDS             complement(2103..2564)
                     /locus_tag="PK856_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRL01738.1"
                     /translation="MQLNRFTDLGIRVLMYLSSIDGPSVTIGRLAEDLQVSKNHLVKV
                     VHFMAQQHWLLTTRGKSGGVALFKRPEEYAIGDVVRVLEQNSIHGERLINCQQPPCVL
                     LPACGLPAILQSALEQFYQFLNQYSLADVVKQPVASILPVIVEHSIINNQH"
     gene            complement(2631..3875)
                     /gene="hmpA"
                     /locus_tag="PK856_04900"
     CDS             complement(2631..3875)
                     /gene="hmpA"
                     /locus_tag="PK856_04900"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HRL01739.1"
                     /translation="MLSTQQIEFIKATVPVLRESGVALTDYFYKRMLNNHPELKETFN
                     MGHQRSGGQAKALAGAVLAYAENIENLAVLGDAVNLIVAKHVSLNIQADQYAIVGENL
                     LHSISEVLGVPMDSDLIAAWAAAYGQLADILIAAEKQRYAEQEAIQGGWNGWREFKVV
                     RKQPESSVITSFYLQAVDGGALPAYQAGQYVSVRVEVPELGIKQPRQYSLSQAANGNE
                     LRISVKRESEREALAAGYVSNTLHEHVQEGDVLELSNPNGDFVLNSADKANVFISAGV
                     GITPMMAMLGQVAAHNMPKATSFIHACRDEHSLSMSEAVAAIKQRFPQLQTYLACETQ
                     PETDLDVQTIGRLNLQTVGAELLPREADYYVCGPVGFMQIQYQSLLGLGIAPEQIHME
                     AFDTGGIAAVTAACGIAAVTAA"
     gene            complement(3985..4719)
                     /locus_tag="PK856_04905"
     CDS             complement(3985..4719)
                     /locus_tag="PK856_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01740.1"
                     /translation="MNYKLITTVLFTAWALSACTTPVLIQDADGRACHQNYEQVKKRF
                     GEHFARPCISDIDRHTGIVEKTKADAVQDLQAASTEGLMQTPPSFSDNSGIYTRSHFQ
                     DGSVYYWMLPYASLQGRGVLDQSREIEARWHNTAPQTVRLYVYHRFNGQDMTNIRFDI
                     DGAVHDYVATWPAEAIRNAGRKNGSVQYFDVPYQVVKGISQAHQAQAWLGYADGSEHA
                     MVLVEQGEPSLAAQGLRRFFNAIETR"
     gene            5013..5237
                     /locus_tag="PK856_04910"
     CDS             5013..5237
                     /locus_tag="PK856_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HRL01741.1"
                     /translation="MCLISGLILTACSWNTYTKEDGTTGLRPTNKFGQSVYYEDGTYA
                     RDQRYNEYRPIRRVLDEEPVSSGNARVHWQ"
BASE COUNT         1310 a         1390 c         1194 g         1465 t
ORIGIN      
        1 cagggaagta ttttttcact tcttgttctg agaacgagta tttggcttca cgcagttttt
       61 cactcacata agtgaaatcc catggttgtg ggtcgtccaa ggccaaggtt tttttggcaa
      121 atgcatacac ctcggctttg tcttgctcgg caaacgggcg tgcttttgcg gccaaatcgc
      181 gcaaaaaggc ggtaacttgg tctggcgttt cggccatttt cgtggccaac gacaattcgg
      241 cataattggc aaaacccaat aattgcgatt cttgtgcggc gatttccaaa atgcgtttga
      301 tgttggcggt attgtcgagt ttggcatcgc ccaattcact cgcacgcgtc acatacgctt
      361 tgtacatctg ctcacgcaaa acacgggtgt ctgagtattg cattacggcc aagtaatgtg
      421 gaatttgcag gccgattttg taacctgttt tgccttccgc ttcagccgct gcggcaaaca
      481 tggccaaggc atcggctggc aaacctgtga gcttgctgtc gcgctccaat accgcttctt
      541 cagcatcagc actgcaaggc gatgaagcag gctgaaccaa acccgccaat tcgtagccat
      601 cggcaaaata caaggcaaac gcatcggtcg catccatcac gttttggctg aattgcgccg
      661 ataattgtgc gctttcggtt tgcaaacccg caaaaatttc ttggttttct gggcttaact
      721 ctgcacctga cagcacaaaa tcacgcaaat cgttgttgag ctttttctgc tcggcagcgc
      781 tcaactgggc aaagtatggc gcagctttaa tggctttaaa gcgggcatac aattcaatat
      841 ccaaagagat ttcagtgaaa aactgggtca cttcaggcat caaaccattg taaaccgcgc
      901 gcaattcagg cgtgtccacc actgaattca ggtgcgccac cacaccccaa atacgaccga
      961 cttgctcggt aatgtcggtc aaacgggtga cggtattgtc ccaagtgatg tcggcttgcc
     1021 ctttcactgc agcgatggca gcgcgcgctt ctgtcatggc ttgtttcatt gcgggctcaa
     1081 tttgtgccac ttctatcgcg tcaaaacgcg gctcatcgtt caattgcagc aaaacattgt
     1141 ctgacataat cggctttctg tgctttaaac attcaatatg cttacttaga taaggctagg
     1201 tgcggcaatt tcaatatcca cgcccgctct gataagccct cactgtaagc aatctgcgct
     1261 aaggcttcaa tgcctcattc actatgactg cgattgtaac ggctccttac atgaaataag
     1321 cggcctgtaa agtttacagg ccgcttattg gctaactgtg atgcctcaat ccatcatgca
     1381 ggtcttattg caattggatg ctgccgctca cattgacgct gatttcggtt tcgccagcca
     1441 tgtcattggc cacggcgcca ccatcggcca tttccatctt ggccatattg gctgtggcgt
     1501 acaggcgtgg aggacgcatg ttgacattgg cattgcccac attcatttcc acgattttgt
     1561 aattgcgtgc gcccaacaca gtcgcaatgt gttgcgcttg ttgttggaaa ttgcgtatcg
     1621 ccttctcggt gagttgcaat tcatattgac gccgcgccgc ggtggttaaa ccataatttt
     1681 gcgattccaa acgtgcatct tgctgcaccg aagccaccaa ttggttgact ttggcggtgt
     1741 cttcactgaa cacgctgaat tgcgcgtaat cgcgccagcc gcgtttttcc actttgttgt
     1801 tcacatactc ggtttgttca ttcaccgaac ggttgagcaa ctggcctttc aggccggctt
     1861 gttcgattgc cgccatcacc cgattaagtt tcacggtgtt gtcacggctt gcatcattgc
     1921 ggttggcggc gttgctttcc accaccaaag tcacgttcaa ttggttttgc ggcacgttca
     1981 tgttggcgct gtcgctcaac tgaatgatgt tgtaatggat gtctttggcc tgtaccaatg
     2041 atgccgcgct caaagccacg cccaataccg caacggtttt ccaaaatgcc ataaatactc
     2101 cttcaatgct gattgttgat gatggagtgt tccacaatga cgggcaaaat gctcgccaca
     2161 ggttgtttca ccacatcggc caggctgtat tgattcagaa actggtaaaa ttgttccagc
     2221 gcagactgca agatagcggg taaaccgcag gcaggcaaca acacacaagg gggttgttgg
     2281 caattgatga gacgttcgcc atgaatgctg ttttgctcca acaccctcac cacatctcca
     2341 atggcatact cctcaggccg cttaaacaaa gcaacaccgc cacttttccc tcttgtggtt
     2401 aagagccagt gttgctgagc catgaaatgc accactttga ccaaatggtt tttagacact
     2461 tgcaaatctt cagccaaacg tccaatcgtc acgctcggcc cgtctatgct gctcaggtac
     2521 atcaaaacgc gtatgcccaa atccgtaaag cgattcaact gcatgctgct tacccatttc
     2581 tctcaaacag cctcgcagca taccgtaaat cgctcatgcg acccaagtcc ttatgctgcg
     2641 gttactgcgg caataccgca tgctgcggtt actgcggcaa taccgccagt atcaaaggct
     2701 tccatgtgaa tttgctctgg ggcaatgccc aaacccaaca gactttgata ctgaatctgc
     2761 atgaatccaa cagggccaca aacataataa tcggcctcac gtggcaacaa ttcagcacct
     2821 acggtttgca agtttaaacg gcctatggtt tgcacatcca aatccgtttc aggctgggtt
     2881 tcacacgcca aataggtttg caattgtggg aaacgctgtt tgatggcagc cactgcttcg
     2941 ctcatgctta agctgtgttc atctcgacac gcatgaataa aactggtggc cttgggcata
     3001 ttgtgtgcag ctacctgtcc caacatggcc atcatagggg taataccaac gcctgcgctg
     3061 atgaacacat tggctttgtc cgcgctgttc aacacaaaat ccccgtttgg gttggaaagc
     3121 tccaatacat ccccttcttg aacgtgctca tgcaaggtat tggacacata gcccgcggcc
     3181 aatgcctcgc gctcactctc acgcttaacc gaaatgcgca gctcatttcc attcgcggct
     3241 tgtgacaacg agtattgacg cggctgttta atgcccaatt caggcacttc cacccgcacc
     3301 gacacatatt gtccagcttg gtatgctggc agagcgccac catccactgc ttgcaaataa
     3361 aacgaggtga tgacactgct ttcaggctgt ttgcgcacca ccttgaattc acgccaacca
     3421 ttccaaccgc cttgaatagc ttcttgttcg gcataacgtt gcttttctgc ggctatcaaa
     3481 atatccgcca attggccata agccgccgcc caagccgcaa tcaaatcgct gtccataggc
     3541 acacccaaaa cctcgctgat ggaatgcagc aggttctcac ccacgatggc gtattggtcg
     3601 gcctgtatgt tcaagctcac gtgtttcgcc acaatcaaat tcaccgcatc gcccaacacc
     3661 gccaagtttt caatgttttc cgcatacgcc aacaccgcgc ccgccaaagc cttggcttgg
     3721 ccaccgctgc gttggtgtcc catgttgaag gtttctttca actcaggatg gttgttcaac
     3781 atgcgtttgt aaaaataatc ggttaaggcc acaccgcttt cacgtaaaac aggcacggtg
     3841 gctttgatga attcaatttg ttgggtgctt aacatgatgt gctttcttca atattgtata
     3901 ataatgtatt ttaaatacat tattaatatt agcacaaaag cggcctgtac gtttacaggc
     3961 cgctttttat ttgatttaca tcaattatcg ggtttcaatg gcattgaaga atcggcgtaa
     4021 tccttgtgct gccagcgaag gttctccttg ctcaaccaac accattgcgt gttcagaacc
     4081 atcggcatag cccagccaag cttgcgcttg atgggcttga gaaatgcctt tgaccacttg
     4141 ataaggcaca tcaaaatact gcaccgagcc atttttacga cctgcattgc gtatcgcttc
     4201 ggcaggccaa gtggccacat aatcatgtac cgcgccatct atatcaaacc gaatattggt
     4261 catgtcttga ccattaaaac ggtggtacac atacaagcgc acggtttgag gtgccgtgtt
     4321 gtgccaacgg gcttcaattt cacggctttg gtctaatacc ccgcgccctt gtaagcttgc
     4381 gtaaggcagc atccaataat acacactgcc atcttggaaa tggctgcggg tgtagatgcc
     4441 actgttgtcg ctgaacgagg gaggcgtttg catcaaacct tcagtcgatg cggcctgtaa
     4501 atcttgtaca gcgtctgctt tggttttttc cacaatacca gtgtgcctgt cgatgtctga
     4561 gatacatggt ctggcaaaat gttcgccaaa gcgttttttc acctgttcat aattttgatg
     4621 acaagcacgc ccatctgcat cttgaatcaa aactggcgtg gtacaggcac tcaaagccca
     4681 tgcagtgaac aatactgtcg taatgagttt gtaattcatg ttctctcttc gctctgaatt
     4741 aatcagtatg gtgtacaaat catacatgag ttaaatgaaa tcattttatg taaatatagc
     4801 gttaactaca cctacatcag cggcttaaag ctgacattat ggtctagact caagtttggc
     4861 ccttaagcgg gattttacct gttcaccaat ctgtggtaaa acgcctcttt ccaaagtggg
     4921 gcttttccgc ttatttgagc ctctacatta aaacttaaaa aatataaaga cataatgcca
     4981 tgaacatagc caaacagata aaacttttat gtgtgtgctt aataagcggc ttgattctca
     5041 ctgcttgcag ctggaacacc tataccaaag aagatggcac cacaggttta cgcccaacca
     5101 ataaatttgg tcaatcggtt tattatgaag atggcaccta tgcccgcgat cagcgttaca
     5161 acgaataccg ccctatccgc cgcgtgttgg atgaagagcc tgtaagttca ggcaatgccc
     5221 gtgtgcattg gcaataagtt aagaactgcg gcatggcaag cgctgcctcc cagtttgttt
     5281 attcaatcca tttttaaagt gtttgtatga atgcttcatt gcatattgtg attttagcgg
     5341 ccggcaaagg cacgcgcat
//
LOCUS       DAOMVK010000105         5347 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_4241_length_5347_cov_2.912698, whole genome shotgun sequence.
ACCESSION   DAOMVK010000105 DAOMVK010000000
VERSION     DAOMVK010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5347
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4241_length_5347_cov_2.912698"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="PK856_04915"
     CDS             <1..631
                     /locus_tag="PK856_04915"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRL01742.1"
                     /translation="AILQIKQQDSDLRVSCEPTLAMKWLIPRLSDFKQRHPQVNVVIL
                     AAGGTVDFATQHIDVAIRRNDFAWPKQVYAEKLADEYMAPVARVGEHQAMCLHSASRP
                     QAWQTWLEQHVSSDSASQYTAMPSTEFEHFYLAIQAAIAGLGTVLVSVYMVADDVSQK
                     ILQAEQAFTADGSGYYLLAMADLFEQEKTTLFLRWLREQMQLTIATHCQ"
     gene            821..1240
                     /locus_tag="PK856_04920"
     CDS             821..1240
                     /locus_tag="PK856_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRL01743.1"
                     /translation="MDKITVKALVKAPIEKVWQAYNDPEDIMQWNTASPDWHTVSSKN
                     DLSVGGQFSHRMEAKDGSMGFDFAGTYTEIVPFKKISYEFGGRTAEIQFQALEDGAIC
                     VQISFDPEQEWPLEQQQAGWQAILDSFATYVEKVKSA"
     gene            complement(1447..2199)
                     /gene="rlmB"
                     /locus_tag="PK856_04925"
     CDS             complement(1447..2199)
                     /gene="rlmB"
                     /locus_tag="PK856_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HRL01744.1"
                     /translation="MAANTRLIFGFHAVNARLWQNPKSITELYVLDGKQDARMREVLE
                     KAGAENVRVILADMDRLNTLSKQARHQGVVGFIDASKNHVDLDDVLENLNEPPFLLIL
                     DGITDPHNLGACLRVADAMGVHAVIAPKDKSAGLNATVSKVACGAAEVVPYITVTNLA
                     RTMRQLKDENIWIVGTAMDGEADLYHYDVPQSVAWVMGNEGDGMRRLTREHCDALVSI
                     PMFGTVESLNVSVSSGMVLSETRRQRVLKQEK"
     gene            2353..2877
                     /locus_tag="PK856_04930"
     CDS             2353..2877
                     /locus_tag="PK856_04930"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HRL01745.1"
                     /translation="MTQKIMVWDGFIRLFHWALVACVVANLFFTSKGETVHQYVGYAA
                     AALVVLRIVWGFVGSKYARFSDFFPTPTLIKAHLCALVKREKEDYLGHNALGALMIFA
                     LIAVILGLGFSGWAQTIDSPYYAEDWPASMHEILVTVLQILVALHVLAVVVMSILTKS
                     DLVGAMFTGRKKVK"
     gene            2910..3398
                     /locus_tag="PK856_04935"
     CDS             2910..3398
                     /locus_tag="PK856_04935"
                     /inference="COORDINATES: protein motif:HMM:NF025918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HRL01746.1"
                     /translation="MDKLNKLLKQYQALLLEDALVGCTHDALVGCTHDDLHKVELYLD
                     VTLPDDFKEISLFCSIDYLGGMPNYQVVRDGNALNLIDETLRIRNGIGLPHEYVFLGE
                     LDTSAILLNTIKQPHVIWVDSIAIERLNQLESHEYDVWENYQDYFEYLLQEEFSERNL
                     DA"
     gene            complement(3486..4466)
                     /locus_tag="PK856_04940"
     CDS             complement(3486..4466)
                     /locus_tag="PK856_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRL01747.1"
                     /translation="MTLFAVPDFRNLSLILRVFVSVSALLLLLPLLSANSEQDYYTHA
                     VNLAAWVAPFVIFTLTLLLLCMRFILSHPYGTVITWMICMVSYLFCAALFKFPTETWF
                     TDMVLVSVYVWGFMHYHAILQRALSPALSEAKLAALTARIRPHFLFNSLNAAIALVRS
                     RPAEAEMVLENLADLFRAQLKDGNQASTLGREIELAQGYLAIETIRMGHQRLQSMWYV
                     DAPEQAEVPHLLLQPLLENAVYHGIEPSHRPGEISISIIKRQDWIYIRIDNPLPEDSN
                     KTKKRQGNQMALANLSDRLTLMYDADATVRSSKQGDIFRVDMRLPYRKAV"
     gene            4518..>5347
                     /gene="argH"
                     /locus_tag="PK856_04945"
     CDS             4518..>5347
                     /gene="argH"
                     /locus_tag="PK856_04945"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HRL01748.1"
                     /translation="MHDNKTWSGRFNEPVSELVKKYTGSIDFDKRLAKWDIDGSLAHA
                     EMLKRVGVLSEQDLAAIQQGMAEIIEDIKSGSLEWSLDLEDVHMNIERRLTDKIGDAG
                     KRLHTGRSRNDQVATDIRLWLRDEITVIQDLIQGLQASLLDLAEQNVDVVMPGFTHLQ
                     VAQPVSFGHHMLAYVEMLGRDFERMVDCRKRVNRMPLGAAALAGTTYSIDRSITAELL
                     GFEQICQNSLDAVSDRDFAIEFTAAASLVMVHLSRLSEELILWMSPRVGFIDIADRFC
                     T"
BASE COUNT         1404 a         1184 c         1354 g         1405 t
ORIGIN      
        1 ggccattctg caaatcaagc agcaagattc cgatttgcga gtttcttgtg agccaacttt
       61 ggcgatgaaa tggctgatac cgcgtttgag tgatttcaag cagcgccatc cgcaggtgaa
      121 tgtggtgata ttggctgcgg gtgggacggt ggattttgcc acccaacaca ttgatgtggc
      181 gatacggcgc aacgattttg cttggcccaa acaggtgtat gcagagaaat tggcagacga
      241 atacatggca ccagtagcac gagttggtga gcatcaagcc atgtgtttgc acagcgccag
      301 ccgtccacaa gcatggcaaa catggctaga gcaacatgtg tcatcagatt ctgctagcca
      361 atatactgcc atgcccagca ccgaatttga gcacttttac ttggccatac aggccgctat
      421 tgcaggcttg ggtacggtct tggtatcggt atacatggtg gctgacgatg tgtcgcaaaa
      481 gatattgcaa gctgagcaag cgtttacagc cgatggttca ggctattact tattggccat
      541 ggctgactta tttgaacaag aaaaaacaac actgtttcta cgctggctgc gtgagcaaat
      601 gcaactgacc atcgcaacac attgccaata atgtgctgag taagtttatt attatggagt
      661 gcctacaaat tttgagcaaa gctgtcacaa ggattgacca ctgcgcagtt tgggcatttt
      721 atggtacaca tacgcttatt ttttcttgtc agcaatgtaa gccttagcta gatttggcaa
      781 gttgaatttc ccatatttca tcaatattaa ggtggtaaaa atggataaaa taaccgtcaa
      841 agctttagtc aaagcgccga tagaaaaagt ttggcaagcc tacaatgacc ctgaggacat
      901 tatgcaatgg aacactgcct cgcccgattg gcatacggtc agctcaaaga atgatttatc
      961 ggtaggcgga cagttttcac atcgcatgga agccaaagat ggcagcatgg gctttgattt
     1021 tgcgggaacc tacacagaaa tagtgccatt caagaaaata tcgtatgaat ttggaggacg
     1081 aactgctgaa attcaatttc aagcgcttga agacggtgcc atttgtgtgc aaatcagctt
     1141 cgacccagag caagagtggc cgctagagca gcaacaagct ggatggcagg ccattttgga
     1201 cagttttgcg acttatgtgg aaaaagtgaa atcggcttga tgaagatgcc tataacaatg
     1261 gagtttgttt agctatctga atattgcata ttggaacatt gactaagcta ctgactgtat
     1321 ctttagtttg ggtttggaat gatgatagtg tcgctagtga tataggctca attcatatgg
     1381 ttaaaacact tacaggccgc attaagcggc ctgtaaatgt ttttcatgca caaatcacac
     1441 taaaacttat ttttcttgtt tcaacacgcg ttggcggcgg gtttcgctca ataccatgcc
     1501 agagctgact gacacgttga ggctttctac tgtgccaaac attggaatcg acaccagcgc
     1561 atcgcagtgt tcacgcgtta agcggcgcat gccgtcgcct tcgttgccca tcacccacgc
     1621 caccgattgt ggcacatcgt aatggtacaa atccgcttcg ccatccatgg cagtgccgac
     1681 aatccagatg ttttcatctt tcaattggcg catggtgcgg gcaaggttgg tgacggtgat
     1741 gtaaggcacg acttctgctg cgccacatgc cactttgctc acggtggcat tcaagcccgc
     1801 cgatttgtct ttcggcgcaa tcacggcatg cacgcccatg gcatcggcta cgcgcagaca
     1861 cgcgcccaaa ttatgcgggt cggtgatgcc gtctaaaatc aacaaaaaag gcggctcgtt
     1921 caggttttcc agcacatcgt ccaaatccac atggttttta gacgcatcga taaagcccac
     1981 cacgccttgg tggcgggctt gtttgctcaa ggtattgagc ctgtccatgt cggccaaaat
     2041 cacgcgcaca ttttccgcgc ccgctttttc caacacctcg cgcatgcggg catcttgttt
     2101 gccgtctaaa acatacaatt cggtgatgga ttttgggttt tgccacagac gggcattgac
     2161 ggcgtgaaag ccaaaaatca ggcgcgtatt ggcggccatg gtgtttcctt ttgtcgttac
     2221 aggccgcatg ttagcacaaa cggctgttta aggtttgttt aagtcggtag ccttaaactc
     2281 aggcgactga acactgtaaa cctgtgcatg ctagaatgtg tgcatatggc ttggtctgat
     2341 gaaggaagta aaatgacgca aaaaatcatg gtgtgggatg gattcattcg tctgttccat
     2401 tgggcattgg tggcctgtgt ggtggccaat ttgtttttca ccagcaaagg cgaaaccgta
     2461 caccaatacg tgggttatgc cgcggcggcc ttggtggttt tgcgcatagt atggggtttt
     2521 gtcggcagca aatacgcgcg cttcagcgat tttttcccta cgccaacgct catcaaagcc
     2581 catttatgtg ccttggtgaa acgcgaaaaa gaagactatc ttggccacaa tgccttgggt
     2641 gcgttgatga tttttgcctt aatcgcagtg attttgggct tgggtttcag cggttgggcg
     2701 caaacgattg actcgcctta ttatgctgaa gattggcctg caagcatgca cgaaattttg
     2761 gtcaccgtat tgcaaatctt ggtggcctta catgtgttag ccgttgttgt gatgagcatt
     2821 ctcaccaaat cggatttggt cggagcgatg tttacagggc gcaagaaggt gaaataaata
     2881 tgaacagcaa gtttggataa ggtatcagag tggataagct aaataaatta ctcaaacaat
     2941 accaagcttt gcttctcgaa gatgcattag taggttgcac tcatgatgca ttagtaggtt
     3001 gcactcatga cgatttacat aaggttgagc tttatttaga cgttacgttg cctgatgact
     3061 tcaaagaaat ttcacttttt tgctcaatag attatttggg aggaatgcca aattatcagg
     3121 tagtaaggga tggcaatgct ttgaatctta tcgatgaaac tctaagaata cgcaatggca
     3181 ttggattgcc acatgaatat gtctttttag gggagttgga tacaagtgcg atacttttaa
     3241 ataccataaa acaaccacat gtcatttggg ttgactccat tgcaatcgag cggttaaacc
     3301 aacttgaatc tcatgagtat gatgtatggg aaaactatca agattatttt gaatatttgc
     3361 tacaagaaga attctcggag agaaatcttg atgcttgaat aaatggtata ttgaaatgta
     3421 aaagcggcct gtaagtaatt tacaggccgc ttttacatga ttcatcaaat tgtttcaaac
     3481 acgttctaaa ccgctttgcg atacggcaag cgcatgtcaa ctcggaaaat atcgccttgc
     3541 ttgctgctgc gcacggtggc atctgcgtcg tacatcaagg ttaagcggtc ggataaattc
     3601 gccaaagcca tttggttgcc ttggcgtttc ttggttttgt tgctgtcttc aggcaagggg
     3661 ttgtctatgc ggatgtaaat ccaatcttgg cgcttgatga tgctgatgct gatttcacca
     3721 gggcggtgtg acggttctat gccatggtaa accgcgtttt ccaacaaggg ttgtaacagc
     3781 aggtgcggca cttcggcttg ctcgggcgca tccacatacc acatggattg caagcgttgg
     3841 tggcccatgc gtatggtttc aatcgccaaa taaccttgtg ccaattctat ttcacggccg
     3901 agtgtgctgg cttggttgcc gtccttgagc tgggcgcgga acaaatcggc caagttttcc
     3961 aacaccattt ccgcttcggc agggcgagag cgcaccaagg caatggcagc attgaggctg
     4021 ttgaacaaga aatgcggacg gatgcgggcg gtcaaagccg caagcttggc ttcactcaaa
     4081 gcgggcgaca acgcacgttg caaaatcgca tggtagtgca tgaatcccca cacatacacc
     4141 gacaccaaca ccatgtcggt aaaccatgtt tcagtgggga atttaaacaa cgctgcacaa
     4201 aacaggtagg aaaccatgca tatcatccaa gtgattaccg tgccataagg atggctcaag
     4261 atgaaacgca tgcacagcaa caacaaggtc aaggtgaaaa tcacaaacgg cgccacccac
     4321 gcggccaaat tcacggcgtg ggtgtaataa tcttgctcgg aattggcgct caataagggc
     4381 aaaagcaaga gcaaagccga taccgagaca aacacacgca aaataagact caaatttctg
     4441 aagtcaggca ctgcaaatag ggtcatgggt tggcgtataa tgcccatttt tggctaactc
     4501 tcgcgaggat gaacagcatg catgataaca aaacttggtc aggtcgtttc aacgaacccg
     4561 tatcagagtt ggtgaaaaaa tacaccggct cgattgattt cgacaaacgt ttggccaaat
     4621 gggacatcga cggctctttg gcacatgctg aaatgcttaa acgcgtcggc gtgttgtctg
     4681 aacaagattt ggctgcgata caacaaggca tggccgaaat cattgaagac atcaaatcag
     4741 gcagcttgga atggtctttg gatttggaag atgtgcacat gaacattgaa cgccgcttaa
     4801 ccgacaaaat cggtgatgcg ggcaagcgtt tgcacacagg ccgctcgcgc aacgaccaag
     4861 tggctaccga catccgattg tggctgcgcg atgaaatcac cgtgattcaa gacttaatcc
     4921 aaggcttgca agcgtcattg ttggacttgg ccgagcaaaa cgtcgatgtg gtgatgccag
     4981 gtttcaccca tttgcaagtg gctcaacccg tgagctttgg tcaccacatg ctcgcttatg
     5041 tggaaatgtt gggtcgcgac tttgagcgca tggttgattg ccgcaaacgc gtcaatcgca
     5101 tgcctttggg cgcagcagct ttggctggca cgacttattc catagaccgc agcatcacag
     5161 cggaattgtt gggctttgag caaatttgcc aaaactcatt ggatgccgtg tccgaccgcg
     5221 atttcgcgat tgaattcacc gcggccgcaa gtttggtgat ggtgcatttg tcgcgtttgt
     5281 cagaagaatt gattttgtgg atgagcccac gcgtgggctt tatcgacatt gccgaccgct
     5341 tctgcac
//
LOCUS       DAOMVK010000106         5314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_4284_length_5314_cov_2.966534, whole genome shotgun sequence.
ACCESSION   DAOMVK010000106 DAOMVK010000000
VERSION     DAOMVK010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5314
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4284_length_5314_cov_2.966534"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="PK856_04950"
     CDS             complement(<1..415)
                     /locus_tag="PK856_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRL01749.1"
                     /translation="MSETITIAAIATAPGRGGVGVIRLSGQNLLPLAATITGGKTPQP
                     RLALYSDFLAADGNPIDNGLLLYFAAPASFTGEDVIELQGHGGPVVMQMLLSRCLELG
                     ARMAEPGEFTKRAFLNNKLDLAQAESVADLIDASSQ"
     gene            complement(511..1704)
                     /locus_tag="PK856_04955"
     CDS             complement(511..1704)
                     /locus_tag="PK856_04955"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL01750.1"
                     /translation="MQKRWIPFTWVCAALLSGTMGTALASPLYPLYQQLWQLQPSDIT
                     TMFIAYMAGTLLSVLFLGRLSDSLGFAKVLRAGLVLVLVGVCLSAIAQNAFMLSSARV
                     IIGIASGLISTSGMMGMLQTSPLPNEAQTARWYATLNGFGFGMGPLIGGVMAQLSDGS
                     VFVPYVPVIILTALVMFQLWRIPFAPPSEPKKTDFKPRLAFPEAGLGRKQFYLAAGGA
                     FAAFAMFSLYASLAGSFIADFLPWHGPAISGGAIAMVLFLSSLTQLSLRRLSLAKTYL
                     AATVMILMANVLLVLTLHLHSVILFALSVIATAMAHGLALMAGFGKVQEVTHKHNRAA
                     VVSTFLVAGYCGAILPVMALGWLADHLGISTAVTLYCAAFMVWAIALFSGFVYLKRRR
                     HALSI"
     gene            complement(2169..4007)
                     /locus_tag="PK856_04960"
     CDS             complement(2169..4007)
                     /locus_tag="PK856_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL01751.1"
                     /translation="MMNKWFTWFESRLQLYPDELPPLSRDNMWAFIRACLKGCGLWIG
                     VMMVFAAGVGAFEAYLFQMMGQLVDAMHTLSPAQLWTEKKGWLLAMLAVLVLSPLWVL
                     TGSSVRLQSLQGVVPMRLRWQFHRLMLGQSMRFYQDEFAGRVSAKVMQTALAVRDMVM
                     IFADMVVYLLIYFVSIGVILAQFDWRLVLPFVLWLFTFAGMLAYFVPKMAAAARNQAD
                     ARSMMTGRITDAYANIATVKLFSHNQRESTYAKEAMDEFIATVHKQMRLGTQIDAVNH
                     SFNMLLITLTAILCVYLWQGGLVSVGAVAAATAMALRLNGLSQWMMWQSAQLFENIGT
                     VQDGMNTLAKDRQVLDVPNAPALQVSHANIAYHNVDFAYEHKQLLKQFNLNIRGGEKI
                     GLVGRSGAGKSTLVNLLLRLYDINGGDISIDGQNIAQVSQVSLRQHIGMVTQDTSLLH
                     RSVRDNILYGRPDATDADLQAALKQAQVCDFLDQLVDAQGRRGLDAHVGERGVKLSGG
                     QRQRIAIARVILKNAPILLLDEATSALDSEVEAAIQENLYSLMENKTVIAIAHRLSTI
                     AQMDRLIVLNEGEIVESGSHEELLSKNGLYAKLWQRQSGGFLGQDD"
     gene            complement(4138..4548)
                     /locus_tag="PK856_04965"
     CDS             complement(4138..4548)
                     /locus_tag="PK856_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRL01752.1"
                     /translation="MSQNNTLTQYQPHALGLLRIVTGYLFIMHCTTKLFQFPYIEMFA
                     NVPVASMFGAAGIIELIGGLLIILGLFTRPVAFVLAGQMAAAYFIGHVADGGNFLMPI
                     LNSGELAVQWCFTFLFFFVAGGGAFALDNLRGKN"
     gene            4698..>5314
                     /locus_tag="PK856_04970"
     CDS             4698..>5314
                     /locus_tag="PK856_04970"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRL01753.1"
                     /translation="MDLLTSIRVFRQVVMRGSFTKAAEDLDMSTAMASKHVTNLEQHV
                     QARLLMRTSRRLHLTEAGQMYFDESGHALDILQQAGANASSGAKQPKGTLKITAPGWM
                     AIPGFARWLSEYHELYPEVMVHLSLENRRVDLVTEGYDLALRVSHDPSPSLIVKPIEN
                     INFFLVASPDYLAKYGTPTKPADLLGHKGVLPTYTNLSNVVMHMD"
BASE COUNT         1396 a         1383 c         1355 g         1180 t
ORIGIN      
        1 attggctgga ggcatcaatc aaatcggcca cgctttcggc ttgggccaaa tcgagtttgt
       61 tgttcaaaaa agcacgcttg gtgaattcgc ctggttcggc catgcgtgcg cccaattcca
      121 agcagcggct gagcaacatc tgcatcacca ccgggccgcc gtggccttgc aattcaatca
      181 cgtcttcgcc tgtgaagctg gcgggcgcgg caaaatacag caacaaacca ttgtcgatgg
      241 ggttgccatc ggcggccaaa aaatcgctgt acaaagccaa gcgtggttgc ggggttttgc
      301 cgcccgtgat ggtggcagcc agtggcaaca ggttttgtcc cgacaaacgg atgacgccca
      361 cgcctcctcg gcctggggcg gtggcaatgg cggcaatggt gatggtttca gacatcttgc
      421 gtaactcaaa taaaaagaat gggaccattg taacgcaaca agcggcctgt aagtttacag
      481 gccgcttgtt tcaaggtgat gcagaagtgt tcagatggat aaggcgtgtc gacgtcgctt
      541 caaatacaca aagcctgaga acaaggcgat ggcccacacc ataaaggcag cacaataaag
      601 ggtaaccgcg gtgctgatgc ccaaatggtc tgccagccaa cccaaagcca tgacgggcaa
      661 gatggcgccg caatagcccg ccaccaaaaa ggtagacacc acagcggcac ggttgtgttt
      721 gtgcgtcacc tcttgcactt tgccaaagcc tgccatcaaa gccaagccgt gtgccatggc
      781 ggtggcgatt acactgagcg caaacaaaat gacggaatgc aaatgcaagg tcaataccag
      841 caagacattg gccatcaaaa tcatgactgt ggccgccaaa taggttttgg ccaagctcaa
      901 acgccgcaaa cttaattggg tgagagagga caagaacaac accattgcaa ttgcgccgcc
      961 actgatggct gggccatgcc aaggcaaaaa gtcagcgatg aatgagcctg ctaaggaggc
     1021 atacaagctg aacatggcaa aggctgcaaa cgcaccaccc gcggccaagt agaattgttt
     1081 gcgtcctaag cctgcttcag gaaaagccaa gcggggtttg aaatccgttt ttttcggctc
     1141 gctgggtggg gcaaaaggaa tgcgccacaa ttgaaacatc actaaggctg tcaagatgat
     1201 gacgggcacg tagggaacga aaacagagcc gtcactgagt tgggccatta ccccgcctat
     1261 taaaggcccc atgccaaagc caaagccatt gagcgtggcg taccaacggg cagtttgggc
     1321 ctcattgggc aaggggctgg tttgcaacat gcccatcatg cctgaggtgc tgattaggcc
     1381 tgaggcaatg ccaatgatga cgcgtgcact ggagagcatg aaggcatttt gagcgatggc
     1441 cgacagacac acgcccacca ataccaaaac cagacctgcg cgcaaaacct tggcaaagcc
     1501 caagctgtcg ctcaagcggc ctaaaaacaa caccgacaac aaagtccctg ccatgtaagc
     1561 gatgaacatg gtggtgatgt cgctgggttg cagctgccac agttgttgat aaaggggata
     1621 aagtgggctg gccaaagccg tgcccatggt gccgctgagt aaggcagcac acacccaagt
     1681 aaagggaatc caacgttttt gcatgatgaa atgacaatca atgtgaaggt gaaatgcggc
     1741 actgacaggc aggccgagtt ttgatggtag tgcgacttgt gcagtgcgtc aatttactta
     1801 agccccgtgg tttaatcaga aattcgccct gatgagccag aaaaacttgt gttgcatcaa
     1861 atgcaaagtc atttggcatg ggtgaatttc gattgtttgc gctaactcaa aaagcgaacg
     1921 cgatgaaacc cgtacattgt gaagcgtgat gacagggcta gctcagacgc acacgcagcg
     1981 aatgaagtga agtggggttt tttattggcg gcggtgattt tggcatgaga cctgttatga
     2041 cgatgatgtg tgtatgaact ttcttcatga tgatgtgact ttgcccggca cttggtgtcg
     2101 ggtttttttt gcacaaaaaa aagcagttga ggagtgctca actgcttttg atatggctta
     2161 caggccgctt aatcgtcttg gcctaagaag ccaccacttt ggcgttgcca caacttggca
     2221 tacaaaccgt ttttgctgag caattcctca tggctgccgc tttcaacgat ttcgccctca
     2281 ttgagcacta tcaatctgtc catttgcgca atcgtagaca aacggtgggc aatggcaatc
     2341 acggttttgt tttccatcaa gctgtacaga ttttcctgta ttgcggcctc aacttctgaa
     2401 tcgagcgcgc tggtggcttc atcgagcaac aaaatcggcg cgtttttcaa aatcacccgt
     2461 gcaatggcga tgcgttggcg ttggccgcct gagagtttga ccccgcgttc accgacatgc
     2521 gcatccaagc cacgacggcc ttgggcatcc accaattggt caagaaagtc gcacacttgc
     2581 gcttgcttca atgcggcctg taagtcggcg tcagtggcat cggggcgacc atacaagatg
     2641 ttgtcgcgca ccgaacggtg tagcaaagaa gtgtcttgcg tgaccatgcc gatgtgttgg
     2701 cgcagcgaca cttggctgac ttgggcgatg ttttgcccgt ctatgctgat gtcgccacca
     2761 ttgatgtcgt acaagcgcag caacaaattc accaaagtgg atttgcccgc acccgaacgg
     2821 ccaaccaaac caattttttc accaccacgg atgttcaaat tgaattgctt gagcaattgt
     2881 ttgtgttcat aggcaaaatc gacattgtgg taagcaatgt tggcatggct cacttgcaaa
     2941 gcgggcgcat tgggcacatc caacacttgg cggtctttgg ccagggtgtt catgccgtct
     3001 tgcaccgtgc cgatgttttc aaacaactgc gctgattgcc acatcatcca ttgcgacaaa
     3061 ccattcaagc gcaaggccat ggccgtggcc gctgccaccg cacccacgct gaccaagccg
     3121 ccttgccaca aatacacaca cagtatcgcg gtcaaggtga taagcaacat gttgaagctg
     3181 tggttgaccg catcaatttg cgtgcccaag cgcatttgct tgtgcaccgt ggcgatgaat
     3241 tcatccattg cctctttggc gtaagtcgat tcgcgttggt tgtgcgaaaa caatttgacg
     3301 gtagcgatgt tggcataagc atcggtgatg cggcctgtca tcatcgagcg ggcatcggct
     3361 tggttgcgcg cggctgctgc cattttcggc acaaaatacg ccaacatgcc cgcaaacgtg
     3421 aacagccaca gcacaaaagg caacaccaaa cgccaatcaa attgcgccaa aatcacacct
     3481 atggacacaa aataaatcag caaatacacc accatgtcgg caaaaatcat taccatatcg
     3541 cgcacagcca aggccgtttg catcaccttg gcagacacgc gacctgcgaa ttcgtcttgg
     3601 taaaaacgca tcgattgacc gagcatcagg cggtgaaatt gccaacgcaa acgcatgggt
     3661 accacacctt gcaggctttg caaacgcacc gatgagccgg ttaacaccca caaaggactg
     3721 agcaccaaca ccgccaacat cgccaacaac cagccttttt tctccgtcca caattgggca
     3781 ggacttaagg tgtgcatggc atcaaccaat tgccccatca tctgaaacaa atacgcttca
     3841 aacgcaccca cgcccgccgc aaacaccatc atcacgccta tccacagacc gcagcctttc
     3901 aaacaagcgc ggataaacgc ccacatattg tcacgcgaca agggaggcaa ttcatctggg
     3961 tagagctgca gccgtgattc aaaccacgta aaccacttat tcatcatatc aaatcacttt
     4021 caaatcttac gcttaagcgc cgtttacagg ccgatattat accttgtctt gcggccgtaa
     4081 atggctttag gccgaccttt tgggtcgacc tgtggtaatg tcatgcaaac agtggaatca
     4141 gtttttgccg cgcaaattgt ctaaggcaaa cgcgccgccc cctgccacaa agaagaacaa
     4201 gaaggtgaaa caccattgca ccgccaattc accactgttt aagatgggca tcaagaaatt
     4261 gccgccatct gccacgtgtc cgatgaaata cgccgctgcc atttggcctg ccaacacaaa
     4321 cgccacagga cgggtgaaca aacccaagat gatgagcaag ccgccgatca attcgatgat
     4381 gcctgccgcg ccaaacatcg atgccacagg cacgttggcg aacatttcaa tgtaaggaaa
     4441 ctgaaataat ttggtcgtgc aatgcataat aaacagataa cctgttacga tgcggagcaa
     4501 gcctaaggca tgaggttggt actgggttaa agtgttgttt tgtgacattt gcgtttcctg
     4561 ttatttgatg attgtgtatg gtatttacag gccgctttgg cagccaaact ttgatgtagt
     4621 gcagtttaac ggcctgaccc tgtgccatct agatgttatt tcggacaaaa ttttttactg
     4681 atggttaaag ataaatcatg gacttattga ccagtattcg cgtttttcgc caagtggtga
     4741 tgcgcggcag ctttaccaaa gcggctgaag acttggacat gtctactgcc atggcaagca
     4801 aacacgtcac caatttggag caacacgtac aagcgcgttt gctcatgcgc accagccgcc
     4861 gtttgcacct caccgaagcg gggcaaatgt attttgatga atcgggccac gctttagaca
     4921 ttttgcaaca ggcaggcgca aatgcgagct ccggcgcgaa gcagcccaaa ggcacactga
     4981 aaatcaccgc cccaggctgg atggcgatac ccggttttgc ccgttggctg tcggaatacc
     5041 acgagttgta tcccgaagtg atggtgcatt tgagcttgga aaaccgccgt gtggatttgg
     5101 tcaccgaagg ctacgatttg gccttgcgcg tgtcacacga cccgtcgcct tcgctgattg
     5161 tgaagccaat cgaaaacatc aatttcttct tggtggcctc gcccgattat ttggccaaat
     5221 acggcacgcc gaccaagcct gccgatttgc tgggccacaa aggcgtgttg cccacttaca
     5281 ccaatttgtc gaatgtggtg atgcacatgg acaa
//
LOCUS       DAOMVK010000107         5304 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_4300_length_5304_cov_3.287674, whole genome shotgun sequence.
ACCESSION   DAOMVK010000107 DAOMVK010000000
VERSION     DAOMVK010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 5304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5304
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4300_length_5304_cov_3.287674"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1247)
                     /locus_tag="PK856_04975"
     CDS             complement(<1..1247)
                     /locus_tag="PK856_04975"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRL01754.1"
                     /translation="MPLMTRRGVLQTLSAGALVSVSPLAFANNAQITAARIWPSDLYT
                     RMTLEASAAVPFKYFVLDNPRRLVVDMQGVSINEVMKTVPSKVLARDPYINQIRVGQF
                     DQNTVRIVVDLKRNISPQVFSLLPVAEYKHRLVIDLFPAGQGSAPAVQAEEEDALMAL
                     LNDYSKGKVKTDGSTTVTAQTTIPKPEDTRPKATPQNKSNINRKIVVVIDPGHGGEDP
                     GAIGPSGLKEKNVVLAISRELKKNLDAAGYKTHLTRNQDVFIPLGVRVATARKLQADL
                     FISVHADAFTSPSARGTGVYALSTSGATSAQAKFLAQTQNASDLIGGVKKVGNVNVDR
                     AILDMTQTASVRDSLVLGKHVLNALGNINKLHKGKVDQAGFAVLKAPDIPSILVETAF
                     ISNPEEERLLAGGAFRGKEINAVS"
     gene            complement(1238..1696)
                     /gene="tsaE"
                     /locus_tag="PK856_04980"
     CDS             complement(1238..1696)
                     /gene="tsaE"
                     /locus_tag="PK856_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRL01755.1"
                     /translation="MADYTQFLADEAASLAFAQQLANMLKPSLTVYLAGDLGAGKTTL
                     TRGLLRAWDFEGTVKSPTYTIVESYELDGFKVEHFDLYRFQSPEEWLDAGLDELFDEH
                     TIALIEWPQMAIGFVPEADLTIALSVQDAGRLCTITPHTELAQKMLAPCL"
     gene            1718..2770
                     /gene="queG"
                     /locus_tag="PK856_04985"
     CDS             1718..2770
                     /gene="queG"
                     /locus_tag="PK856_04985"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HRL01756.1"
                     /translation="MNATELSLAVCTEAKRLGFLDCKISQPHLSAEHDEKLRAWLAQG
                     YEGEMTFFHKHGTARNHADYVLPEAQSVIMVALPYWTEDFDQTRAILADSHAAYVSRY
                     AVGRDYHKVLRSRLKQLAQYLSTLVPEGIHRPVVDSAPVSEVSFAVQAGLGWRGKHTL
                     LLQKNTGSFYFLGALLTTVKIEPTEAINGHCGQCSRCIQACPTGAIVGPHTVDARRCI
                     SYLTIELPTIVPEPFRKAMGNRIYGCDDCQLVCPFNRFTPDSTEADFQVRHNLNQVSL
                     LDLLAWTEDDFKERMAGSPIYRIGFHKWLSNILIALGNADSHADIAASILPFCSHESP
                     LIAEAAQWASAQHSPA"
     gene            2888..3691
                     /locus_tag="PK856_04990"
     CDS             2888..3691
                     /locus_tag="PK856_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HRL01757.1"
                     /translation="MTFQLIIIGDEIIHGSRVDAHFSAIKTLLTERGLRLHSVQYLPD
                     DRALITAQLKRSFADGIPTFVTGGIGGTPDDHTRQAAAAALNLPLALHAGAVPAIEAI
                     AERRGESLDNPNHLTRLNMATFPEGADLVPNPYNNIAGFSMHEHYFLPGFPVMAHPMV
                     AWVLDTVYPHLHHQTRYASVSVWVYNLPESNITEMMQTLEAEHPHIQTFSLPSTLQAE
                     HGSPYQYRIEFGLKAQDEACDTLPAVWEQVLQQLRDMGGELQAIAQPSE"
     gene            complement(3894..4922)
                     /locus_tag="PK856_04995"
     CDS             complement(3894..4922)
                     /locus_tag="PK856_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRL01758.1"
                     /translation="MAAQKTCRLDLALQGGGSHGAFTWGVLDALLEDGRFEFDGISGT
                     SAGAMNAVVLAAGFAEAKTEGLSSAAAHRLRCDLAREKLHTFWHEVGKIGTLLAASPA
                     SFAAQMMPWLKPVLSPAQSNPFDINPLRRILNQLVDFDLIARLHGEKWMPQLFICATS
                     VRTGKGEIFANRNLSSQVVMASACLPLLYQAVEIDGEQYWDGGYAGNPALYPLIYHTP
                     ANDILLVQINPVAIDQMPNSLDEIQDRVNQITFNAGLLSELRAIRFVTRLLEEQKLDT
                     SRYRKIFLHQIDGGSALMAYGSDSKVKSDWEFLQTLFEQGRVAGEQWLKTDVKYLGVK
                     NTMPKLLD"
     gene            complement(4926..5117)
                     /locus_tag="PK856_05000"
     CDS             complement(4926..5117)
                     /locus_tag="PK856_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01759.1"
                     /translation="MEDEFTIAAIFSRTKYRFIVYLCTKIIKFGSGMGRDDGGERSPA
                     IKALDAVKMAAMKKQINMG"
BASE COUNT         1312 a         1471 c         1243 g         1278 t
ORIGIN      
        1 gaaactgcgt ttatttcctt gccgcgaaac gcaccgcctg cgagcaagcg ctcttcttcg
       61 ggattggaaa taaacgcagt ttccaccaag atagacggaa tgtctggcgc tttcaatacc
      121 gcaaagcccg cttgatcgac tttacctttg tgcaatttat tgatattacc caaggcattc
      181 aacacatgct tgcccaacac caatgagtcg cgcacagagg cggtttgggt catgtccaaa
      241 atggcacggt ctacgttcac attgccgact ttcttcacgc cgccgatcaa atctgaggcg
      301 ttttgggttt gcgccaaaaa tttcgcttgc gcactggtcg cgccgctggt gctcaaggca
      361 tacacacccg taccgcgtgc agatggactg gtaaatgcat ctgcatgcac tgagatgaac
      421 aagtcggcct gtaatttgcg cgccgtcgcc acgcgcacac ccaaaggaat gaacacatct
      481 tggttgcgcg ttaagtgggt tttgtagcct gccgcatcga ggtttttctt caattcacgg
      541 ctaatggcca agaccacgtt tttctctttc aagccgctgg gccctatcgc acctgggtct
      601 tcaccgccgt ggcctgggtc tatgaccacc acgattttgc ggttgatgtt ggatttgttt
      661 tgcggcgtgg ctttgggacg cgtgtcttca ggttttggga tggtggtttg cgccgtgacc
      721 gtggttgagc catcggtttt gaccttgcct ttgctgtaat cattgagcaa agccatcaat
      781 gcatcttctt cttcggcctg taccgcaggt gcggagcctt gtcctgcggg gaacaaatct
      841 atcaccaagc ggtgtttgta ttcggccaca ggcaacaaag aaaacacttg cgggctgatg
      901 ttgcgcttca aatccaccac gatgcgcacg gtgttttgat caaactggcc gacgcgaatt
      961 tggttgatgt aaggatcgcg tgccagtact ttgctgggca cggttttcat cacttcgttg
     1021 atgctcacgc cttgcatgtc caccaccaag cggcgtggat tgtcgagcac aaagtatttg
     1081 aacggcacag ccgcgctggc ttccaaagtc atgcgggtgt acaaatcaga aggccagata
     1141 cgggcagcgg ttatttgggc attgttggca aaggccagtg gactcacaga caccagagcg
     1201 ccggcactta aggtttgcaa cacaccacgt cgggtcatca aaggcatggg gctaacatct
     1261 tttgagcaag ttctgtatgg ggggtgattg tacacagacg accagcgtcc tgtacactca
     1321 aggcgatggt gaggtcggcc tcaggcacaa agcctattgc catttgtggc cattcaatca
     1381 aggcgatggt gtgttcatca aacaactcat ccaagcccgc gtccagccac tcttctggac
     1441 tttgaaagcg gtacaaatca aagtgctcca ctttaaagcc atccaactca taactctcaa
     1501 caatggtgta agtcgggctt tttaccgtgc cctcaaaatc ccaagcgcgc aacaagccgc
     1561 gggtgagtgt ggttttgcct gcccccaaat caccggccaa atacacggtt aatgagggct
     1621 tgagcatgtt tgccaattgt tgtgcaaatg caaggcttgc cgcttcatcg gcaaggaatt
     1681 gcgtataatc tgccatgttg tttccataga tagaagtatg aatgccaccg aattgagtct
     1741 ggctgtgtgc acagaagcca aaagactggg ttttttagac tgcaaaattt cgcagcccca
     1801 cttgtcagcc gaacacgatg aaaaactgcg tgcgtggctg gctcaaggct atgaggggga
     1861 aatgacgttt ttccacaaac acggcacagc gcgcaatcac gccgattatg tgttgccaga
     1921 agcccaatcg gtcatcatgg tcgccttgcc gtattggacg gaagactttg accaaacacg
     1981 cgccattcta gcagatagcc acgctgcata tgtgtcgcgc tatgccgtag gacgggatta
     2041 tcacaaagtt ttacgttcac gcctcaagca attggcacaa tatttatcca cgctcgtgcc
     2101 cgagggcata cacaggccag tggttgacag cgcccctgtg tcagaggtgt cgtttgcggt
     2161 acaagctggt ttgggttggc gtggcaaaca cactttgctt ttgcaaaaaa acactggcag
     2221 cttttacttt ttgggcgctt tgctcaccac agtaaaaatc gagcccaccg aagccatcaa
     2281 tggccattgc ggtcaatgca gtcgctgcat ccaagcctgt cccactggtg ccatcgtagg
     2341 cccacataca gtggatgcac ggcgctgtat ttcttacctc accatcgaat tgcccaccat
     2401 cgtgcccgag ccttttcgca aagccatggg caaccgcatt tatggttgcg acgattgcca
     2461 attggtgtgc ccatttaacc gtttcacccc cgacagcacc gaagcggatt ttcaagtgcg
     2521 gcacaatttg aatcaagtga gtttgctaga cttattggct tggacggaag acgatttcaa
     2581 agagcgcatg gcaggcagcc cgatttaccg cattggcttt cacaaatggc tgtccaatat
     2641 tttgattgcc ttgggcaacg cagacagcca tgccgacatc gccgccagca ttctcccatt
     2701 ttgcagccat gaatcgccgc tgattgccga agccgcgcag tgggccagcg cacaacacag
     2761 ccctgcataa atcgtcccgc cacacataga caaacccagc cgtttcacct actgtatgca
     2821 cttgactcag cagccatgac ccgcgctgct ttccactcca tttaagcggc ctgtaaaaga
     2881 ggaatgtatg acttttcaat tgattattat tggtgacgaa atcatccatg gcagccgcgt
     2941 ggacgctcat ttcagcgcca tcaaaacctt attgaccgag cgaggtttgc gtttgcacag
     3001 cgtgcaatac ctgccagacg accgcgcctt gattaccgcg caattaaaac gcagctttgc
     3061 cgatggcatc cccacttttg tgactggcgg catcggcggt acacccgacg accacacccg
     3121 tcaagccgca gccgcagccc tgaatttgcc tctggccttg catgcaggtg cggtgcctgc
     3181 cattgaagcc attgctgagc gccgtggcga aagcttggac aaccccaatc acctcacgcg
     3241 cctgaacatg gccacctttc cagaaggcgc agatttagtg cctaatccgt ataacaatat
     3301 cgctggtttt tcgatgcacg agcattattt tctgcctggc tttcctgtga tggcacaccc
     3361 gatggtggct tgggttttgg acacagtgta tccacaccta caccaccaaa ctcgctatgc
     3421 cagcgtcagc gtgtgggtgt ataacctgcc cgaatccaac atcaccgaaa tgatgcaaac
     3481 cctagaagcc gagcatccgc acattcaaac cttcagcctg ccctcaactt tacaggccga
     3541 gcatggcagc ccttaccaat accgcattga atttggcttg aaagcccaag acgaagcctg
     3601 cgacaccttg cctgcggtgt gggaacaggt tttgcagcaa ctgcgtgaca tgggagggga
     3661 attgcaagca atcgcccaac ccagtgaata agccccctgt aaaaccatca acagcctctg
     3721 ctgtactcag cagaggctgt tttaaatcat ttaagccaac accaaaccca tctccaatca
     3781 caaagcaaac atcaaacctg acctctttgt aaaatcaacc atggcgcctc agccatttca
     3841 taccacttac agctaccaca agcatacacg cagcgtcctg actcaatgac tgactaatcc
     3901 aatagcttgg gcatggtatt tttcaccccc aagtatttga catcggtttt cagccattgc
     3961 tcacccgcaa cccgcccttg ctcaaacaat gtttgcaaaa actcccaatc cgatttcacc
     4021 ttgctgtcgc taccataagc catcagcgcg gaaccaccgt caatctgatg caaaaagatt
     4081 ttgcggtagc gactggtgtc caatttttgc tcttctaaca aacgcgtgac aaaacggatg
     4141 gcacgcaatt cagataacaa gcctgcatta aacgtgattt gattgaccct gtcttgtatt
     4201 tcatcgaggc tgttgggcat ttgatcaatg gcgacagggt tgatttgtac caataaaata
     4261 tcgttggcag gcgtatggta aatcaaggga tacaaagcag gattgcccgc ataaccgccg
     4321 tcccaatact gctcaccatc gatttctacc gcttgataaa gcagtggcaa acacgcagat
     4381 gccatcacca cttggctgct gaggttgcga ttggcaaaaa tctcaccttt gccagtgcgc
     4441 acactggtgg cacaaataaa caattgtggc atccactttt caccgtgcaa acgggcgatc
     4501 aaatcgaaat ccaccaattg attgagtatg cggcgcaagg gattgatgtc aaaagggttg
     4561 ctttgcgcag gagacaaaac aggtttgagc cacggcatca tttgcgcggc aaatgatgca
     4621 ggacttgcag ccaaaagcgt gcctatcttg cccacttcgt gccaaaatgt gtgcaacttt
     4681 tctctggcca aatcgcagcg caagcgatgg gcagccgccg agcttaaacc ttcggttttc
     4741 gcttcggcaa atcccgccgc caacaccacc gcattcattg cgcctgcgct ggtgccactg
     4801 atgccatcaa attcaaaacg gccatcttcc aacaaggcat ccaatacccc ccaagtgaat
     4861 gcgccatgtg aaccacctcc ttgcagcgcc aagtccaaac ggcatgtttt ttgagcagcc
     4921 atacttcacc ccatgtttat ctgtttcttc attgctgcca tcttaacagc atccagagcc
     4981 ttaatggcgg ggcttcgctc accaccgtca tctctgccca tacctgagcc gaatttaata
     5041 atttttgtgc ataaataaac aataaaacgg tatttagtgc ggctaaaaat cgctgctata
     5101 gtgaattcat cttccatttt gcactgcaac agaaagccgc acatgctcaa attcaacaaa
     5161 tcccaactct tggcactccc tttggtattg gcattggcag cctgcggcaa tcaagcaagc
     5221 aacgacaaaa cagcggcaag caataccgaa ggtagccaag ccacttcagg cagcagcgtc
     5281 agcattttaa acgtgtctta cgac
//
LOCUS       DAOMVK010000108         5280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_4326_length_5280_cov_3.253780, whole genome shotgun sequence.
ACCESSION   DAOMVK010000108 DAOMVK010000000
VERSION     DAOMVK010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5280
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4326_length_5280_cov_3.253780"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1959)
                     /locus_tag="PK856_05005"
     CDS             complement(<1..1959)
                     /locus_tag="PK856_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL01760.1"
                     /translation="MSTLVKKNWGNALSAFADKRAAIMFFLGFSAGIPILLIFSSLSL
                     WLGEAGVDKTAVTMFSWAALGYSFKFVWAPLVDALPIPVLTKMLGRRRSWLLLAQVLI
                     MVAIGVMAMTNPALGQQALIQMAIGAVLLGFASATQDIVIDAYRIELADTQMQTVLAS
                     TYNAGYRIGMIVAGAGALFIASGFGTAKGNYIYSAWQYTYWIMAAVMLIGVATTLLIQ
                     EPKIKREMKQYQKTDYLRLVLVFACVVVGFVSCFIALGAVTALISVSDPFLSFLVEAC
                     RFIASGVAAVMVGVLLVKLGAVNKQMAAETWVVPISDFFSRYGVKLALSLLLLIGFYR
                     ISDIIAGVISNVFYQDMNFTKEEIAEAVKVYGVIFSLVGGFFGGLLSQRFNIMRVMFI
                     GAILASCTNLIFIGLVNSGTKTSAVTVTIGGFTQQVVTDEVGVWTLEVPTSVLQAAHS
                     IETKVSTADNNGRVITRQMPYLLAKGNKTVQIQWLPLTRDNMLSQQEAQEKAIVLEGQ
                     VFGIAAADLEKTTVDVMLGQDTFVAKVDKNGLFSTTVSGKQLAQAQVRTLQAQVNGPA
                     GVVNAQLDYEIGETAQISKLDNHIDAMPLVQADSATHTVSGKVVKQYSQLWLYFAIIM
                     DNLAAGLAGAAFIAFLSSLTSVSFTAVQY"
     gene            complement(2115..3374)
                     /locus_tag="PK856_05010"
     CDS             complement(2115..3374)
                     /locus_tag="PK856_05010"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRL01761.1"
                     /translation="MAVHIQEVLGQYVGPILAQYAQNDLGLDEAHAFKSVEKAIPAVL
                     AGVISHVSDEHAAKRFFNLLTGTNVNTKWLEAGQLQGEALAPVQKLGGQWLGLLFGGR
                     ASEVEQAVAQDAGIDAPQASGIMALAIPTVLAALKQWIQQNQYGQHQFYGQLVAQESF
                     FSKIFSPKLLAALGVPSALTLVAGIRSWGDQYFADKATVAATAESAPLRTSVPPVAPS
                     ATSAPAAGSMAWAKWVLGIIVLGALAWMGKAVFMDGQTPSAPVASAPVVASAPVVASP
                     ALTASVPVASDVSASVPVAASSVAAASEVADTATDRTVYENGALKFYFATGSAKISDD
                     AKDLAEPLLAAAKEGKKLRISGYNDATGDAESNEWLAKKRAQAVRSFLNKQGVSKSQL
                     ELVKPEATTSQSGNMAQDRRVEVTIVE"
     gene            complement(3429..4073)
                     /locus_tag="PK856_05015"
     CDS             complement(3429..4073)
                     /locus_tag="PK856_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRL01762.1"
                     /translation="MTLQVEIIPVTPFQQNCCLLWDADTKEAVLTDVGGNADMLWARI
                     QELGLDLQQIWLTHGHLDHAGGVMDLRQLKEVPVIGPHPDDAFWLDQLPEATAKYGFP
                     VSPVVQPTQWLSEGDEVKVGAHVFQVLHIPGHTPGHVVFYSAEYGLIIGGDILFQGSI
                     GRTDFPRGNHQDLISNIQQKLYTLPDATEVIPGHGPMTSIGQEKRTNPYVRAAD"
     gene            complement(4206..4700)
                     /locus_tag="PK856_05020"
     CDS             complement(4206..4700)
                     /locus_tag="PK856_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HRL01763.1"
                     /translation="MALPWSPQACATSVTDTGSQCLQAAVIMMLMAAFCMGSDVLTKQ
                     MWRRSLCVLGLSMVALTAGAQTVQVACPENVVVGQSYTMQLKAKLSTGYRWILMQPTE
                     DLHIHESDPEPVDSEGGDTEHVLQTWKMQAQSPLATDLVWLYARPEQLSKTVLTASCH
                     LVAK"
     gene            complement(4654..>5280)
                     /gene="purH"
                     /locus_tag="PK856_05025"
     CDS             complement(4654..>5280)
                     /gene="purH"
                     /locus_tag="PK856_05025"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448532.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRL01764.1"
                     /translation="AFNCEVDAATAEAITSRQFMEVLMAPKFTADALAVLEAKKNVRV
                     LEVPLSEGTNRFEIKRVGGGLLVQTPDIHVLTREDCTVVSERQPTEQEWNDLLFAWNV
                     AKYVKSNAIVFCKNGQTHGVGAGQMSRVDSTRIAARKAEDANLPLEAAVAASDAFFPF
                     RDGIDVIAEQGIKAIIQPGGSMRDEEVVAAANEHGIAMVTTGVRHFRH"
BASE COUNT         1335 a         1484 c         1177 g         1284 t
ORIGIN      
        1 atattgcacc gcagtgaaag acacactggt taaactggac aagaaggcaa tgaatgcagc
       61 cccagccaag cctgcggcca aattgtccat gataatggca aaatacaacc acaactgact
      121 gtattgtttg actacttttc ccgatacagt gtgagtagca ctgtcggcct gaaccaatgg
      181 catggcatca atgtgattgt ccaacttact gatttgagca gtttctccaa tttcataatc
      241 caactgagcg ttgacaacac cagctgggcc attcacttgc gcttgcaatg ttctcacttg
      301 agcttgggcc aattgcttac ccgataccgt ggttgaaaac agtccgtttt tatccacctt
      361 ggccacaaat gtatcttgac ctaacatcac atctaccgtg gttttttcca aatcggcagc
      421 agcaattcca aacacctgtc cttcaagtac gatggctttt tcttgcgctt cttgttgaga
      481 aagcatgtta tcacgcgtca aaggcagcca ttgtatttgt actgtcttat tacccttggc
      541 caacaaatat ggcatttgtc ttgtgatgac acgaccattg ttatcagcag tggacacttt
      601 ggtttcaata ctgtgagcgg cctgtaacac tgaagtcggt acctctagtg tccacacccc
      661 cacttcatca gtgaccactt gctgggtaaa acctcctatt gttaccgtga ctgccgaagt
      721 ttttgtaccc gaattgacca aaccaatgaa aatcaaattg gtacaactgg ccaatatcgc
      781 cccaataaac atcactctca tgatgttaaa acgctgcgat agcaatccac caaaaaagcc
      841 acctaccaaa gaaaaaatca ccccatacac cttcaccgcc tcagcaattt cctctttggt
      901 gaaattcatg tcttggtaaa acacattgga aatcacccct gcaatgatgt ctgaaatacg
      961 gtaaaaaccg attaacaaca acaatgacaa agccaatttc accccatagc gactgaaaaa
     1021 atcgctgatg ggcaccaccc aagtttccgc cgccatttgc ttgttgaccg cacctaattt
     1081 caccagcagc accccaacca tcaccgctgc cacacccgaa gcaatgaatc gacaagcctc
     1141 aaccaaaaaa gacagaaaag ggtcactcac tgaaatcagc gcagtcaccg cgcctaaagc
     1201 aataaaacaa ctgacaaaac ccaccaccac acaagcaaaa accaacacca aacgcagata
     1261 atctgttttt tggtattgct tcatttcgcg cttgattttg ggctcttgta tcaacagagt
     1321 tgtggcaaca ccaataagca tcactgcggc cataatccaa taagtgtatt gccaagcact
     1381 gtaaatataa ttgcccttag cggtaccaaa tcctgatgca ataaataagg cacccgcacc
     1441 tgcaactatc atgcctatgc gatagcctgc gttgtaagta gaggccaaaa cggtttgcat
     1501 ttgcgtgtcg gccaattcaa ttcgataggc atcaatcacg atgtcttggg tagcagaagc
     1561 aaagcccagc aacaccgcac ctatggccat ttgtatcaaa gcctgctgac ctaaagcagg
     1621 atttgtcatc gccatcaccc caatggccac cataatcagc acttgtgcca gcaataacca
     1681 tgagcgcctg cgccctaaca tcttagtcaa cacgggaatg ggtaaggcat ccaccaaagg
     1741 tgcccaaacg aatttaaatg aataccccaa agccgcccaa ctgaacatgg tgaccgccgt
     1801 tttgtccaca cccgcctctc ctaaccacaa agacagactg gaaaaaatca ataaaatagg
     1861 aataccggca gaaaagccta aaaaaaacat gatggcggca cgtttgtcag caaatgcaga
     1921 caaagcattg ccccaatttt ttttcactag agtgctcatt gataacgcat tggcttgatt
     1981 aaattgatgg gaattctacg ctaaaaatgt ggccatcgct gtttattcac tgctcacagg
     2041 atgccacctc attaaaaatg cggcctgtaa atccatttac aggccgcttg tttcttgctt
     2101 taaatcactg ctgcttattc aacgatggtc acttccacac ggcggtcttg tgccatgttg
     2161 ccgctttgac tggtggtggc ttcaggtttc accaattcca attggctttt tgacacgcct
     2221 tgtttgttga ggaaactgcg caccgcttgg gcgcgttttt tcgccaacca ctcgttgctt
     2281 tcggcgtcgc ctgtggcgtc gttgtatccg ctgatgcgca atttcttgcc ttctttggcg
     2341 gccgccaaca aaggttcggc caagtctttg gcatcgtcgc tgattttcgc gctgcctgtg
     2401 gcgaagtaga atttcaaggc accgttttca tacacagtgc ggtctgtcgc ggtatcggca
     2461 acttcactgg ctgctgccac gctgctggcc gccactggta cactggcact cacgtcactg
     2521 gccaccggca cgctggcagt caaagctggg ctagctacca caggggcgct ggccacaaca
     2581 ggagccgaag ccactggcgc gctaggggtt tgcccgtcca tgaacacggc tttgcccatc
     2641 catgccaatg cgcccaacac gatgatgccc aatacccatt tggcccaggc catgctgccc
     2701 gcggctggtg cgcttgtagc actgggcgcc actggcggca cactggtccg caagggtgcg
     2761 gattcggcag ttgccgccac cgtggctttg tcggcaaagt attggtcgcc ccatgagcgt
     2821 atgcctgcca ccaatgtcag ggcactgggc acacccaagg ccgccaacaa ttttggactg
     2881 aaaattttgc tgaagaacga ttcttgcgcc accaattgac cataaaactg gtgttggcca
     2941 tattggtttt gttgtatcca ctgtttcaac gccgccaata cggtcggaat ggccaaagcc
     3001 atgatgccgc tggcctgtgg cgcatcgatg cctgcgtctt gtgccaccgc ttgctccact
     3061 tctgaagcgc ggccaccaaa caacaaaccc aaccactgcc cacccagttt ttgcacaggc
     3121 gccaaagcct ctccttgcaa ctggcctgcc tccaaccact tggtgttgac attggtgcct
     3181 gtgagcaagt taaaaaagcg cttggccgca tgctcatcac tgacatggct gatcacacct
     3241 gccaacacgg caggaatggc tttttctact gatttgaacg catgggcctc atccaaaccc
     3301 aaatcgtttt gcgcgtattg cgccaaaatc gggccaacgt attgccccaa cacttcttga
     3361 atatgcactg ccatattcgc ggtctccata tcaaaataac tgcgtctgca aaggtttaca
     3421 ggccgctttt aatctgctgc gcgcacataa ggattggtgc gcttttcttg gcctatgctg
     3481 gtcatcggcc catgccctgg tatcacctcg gtggcgtcgg gcaaagtata cagtttttgc
     3541 tgaatattgc tgattaaatc ttggtggttg ccacgcggaa agtcggtgcg accaatcgag
     3601 ccttgaaaca aaatgtcgcc accgatgatt aagccgtatt cagccgagta aaacaccaca
     3661 tgtcctggcg tgtggccagg aatgtgcaac acttgaaaca catgcgcacc cactttgacc
     3721 tcgtcacctt cactcagcca ctgcgtgggt tggaccactg gtgacacggg aaaaccatat
     3781 ttggccgtgg cctcaggcaa ttggtctagc caaaacgcgt catcaggatg cggacctatg
     3841 acgggcactt ctttcaattg gcgcaagtcc atcaccccac cagcatggtc taggtgaccg
     3901 tgggtcaacc aaatttgctg caagtccaaa cccagctctt ggatgcgtgc ccacaacatg
     3961 tcggcattgc cgcccacatc ggtcagcacc gcttctttgg tgtcggcatc ccacagcaga
     4021 caacagtttt gctgaaatgg ggtcacgggt atgatttcaa cttgtaaggt catgcttatt
     4081 ccttcgtatt gctgtgatga cagcaatcaa tgctttcaca aaaaataatg ggctgacagg
     4141 gcaaataagc ggcctgtaaa cctgaaaacc ttcttatttg tggctgtggt gtgtttactt
     4201 agagcttact tagccactag atggcagctt gcggtgagca ccgtcttgct caattgctca
     4261 ggccgcgcat acagccaaac caaatcggtg gccaatgggc tctgcgcttg catcttccaa
     4321 gtttgcaaaa catgctcagt atcgccacct tctgaatcca cgggttcagg gtctgactca
     4381 tgaatgtgca agtcttcggt gggctgcatc aaaatccagc ggtaaccagt gctcaatttc
     4441 gccttcagct gcatggtata actttgtccc accaccacgt tctcagggca agccacttgc
     4501 acggtctgcg cccctgccgt caatgccacc atgctcaagc ccagcacaca caaacttctt
     4561 cgccacattt gctttgtcaa cacatcgctt cccatgcaaa aagccgccat taacatcatg
     4621 ataacagcgg cctgtaaaca ttgtgaaccc gtatcagtga cggaagtggc gcacgcctgt
     4681 ggtgaccatg gcaatgccat gctcattggc agcggcaacc acttcttcat cacgcataga
     4741 gccacccggt tggatgatgg ctttgatgcc ttgctcggca atcacatcaa taccgtcacg
     4801 gaatgggaag aaggcatcgg atgcagcaac agccgcttcc aaaggcaggt tggcatcttc
     4861 ggctttgcgt gcggcaatgc gggtagaatc cacgcggctc atttgacctg cgcccacgcc
     4921 atgggtttgg ccgtttttgc agaacacaat cgcattggat ttcacgtatt tggccacatt
     4981 ccaagcgaag agcaaatcgt tccactcttg ttcggtcggt tggcgctcag acacaactgt
     5041 gcagtcttcg cgggtcaaca catggatgtc aggggtttgt accaacaagc cgccgcccac
     5101 gcgtttgatt tcaaagcggt tcgtgccttc gctcaaaggc acttccaaca cgcgcacgtt
     5161 tttcttggct tctaacaccg ccaaagcatc ggcagtgaat ttaggcgcca tcaacacttc
     5221 catgaattgg cgtgaggtaa tcgcttcggc agtggccgca tccacttcgc aattgaaggc
//
LOCUS       DAOMVK010000109         5276 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_4332_length_5276_cov_3.368129, whole genome shotgun sequence.
ACCESSION   DAOMVK010000109 DAOMVK010000000
VERSION     DAOMVK010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 5276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5276
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4332_length_5276_cov_3.368129"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="PK856_05030"
     CDS             <1..852
                     /locus_tag="PK856_05030"
                     /inference="COORDINATES: protein motif:HMM:NF015150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HRL01765.1"
                     /translation="KDALSMTNSPHFRGYSRVNEEHTRNQPDFREQIDIGADLEAVEF
                     DANTPLWLKLQGPNQWPENWPEFKTIVTAWQTDLRRIAITLLRAFTLALGLPEDALDQ
                     FVTGKPNELLKLIHYPSAELNEGKNQGVGAHKDAGILTLLLQDQVGGLQVLSKQDGWI
                     EAPYVEGAFIVNIGEILELATNGYLVANMHQVVSPKAHVDRYSIAYFITPNLYAGEVP
                     ILELPAALQQLATGPQSDPLNPLLKNAGENTIKGRLRSHLKVTEKFYPEEYQRILNQR
                     QQQEVTA"
     gene            849..1718
                     /locus_tag="PK856_05035"
     CDS             849..1718
                     /locus_tag="PK856_05035"
                     /inference="COORDINATES: protein motif:HMM:NF015159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HRL01766.1"
                     /translation="MIHAIKKSIAILSLLAVVACSNSADKPAASTPAAAKTDTTKIVI
                     GSMGSDSDIWRFIAKSPAAQEAGLDIEVKDINDGITLNNATAEGQVDVNAFQSWAYFK
                     SFNQTNGDKLGALATTYLEPMGLYSKTHKDLNALPQGATIAIPNDTANTSRALRLLEE
                     AKLITLKADFNPVTGTVQDIADNPKKLNIKLVQGASGPRVLSDVDLGAIGNTNALESG
                     LNVTKDALFHEQVGDRVLNNINILATKKDRVNDAVLQKLSALYHQPAVDAYIKEHFGG
                     TKLDVNQPISSLN"
     gene            1811..3094
                     /locus_tag="PK856_05040"
     CDS             1811..3094
                     /locus_tag="PK856_05040"
                     /inference="COORDINATES: protein motif:HMM:NF005455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HRL01767.1"
                     /translation="MSNTPYRRDLNGEDLSPETLMMSYGYDPKLSEGSIKPPIFLTST
                     FVFKSAEEGKDFFNIVSGRTPAPAEQKNGLVYSRFNQPNSEIVEDRLALYERGEDAAL
                     FASGMAAITTSLLAFVQPGDVILQSQPLYGGTETLLLGLFKRLGIDSIHFDNGCDAYS
                     VRAAIAQVPAGRRIVAIYVETPSNPTNTIVDIALLANIAQELAQRQNGDKPLLICDNT
                     LLGPVFQRPLEHGADLSVYSLTKYVGGHSDVVAGAAIGSKALLKKIKSQRSAIGTQLD
                     PHSCWMLGRSLETLALRMDKANANAAQLAQFLHAHPKIKRVHHLDFLAENSPEYSVHQ
                     SQSSGAGSTFAIEVGDDEAAAFRFLNHLAIFKLAVSLGGTESLASHPASTTHSGIAAD
                     VRARIGIGDNLVRLSIGIENANDLQRALSLALEAV"
     gene            3242..3829
                     /locus_tag="PK856_05045"
     CDS             3242..3829
                     /locus_tag="PK856_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01768.1"
                     /translation="MPNALRQNLFLLPASAHSSLLRMSWRPLVACYGFMLLASMIGLG
                     VDAMHHPDLQHMAYDSWQLSWRHSQWAVACLLFLMVFVRPKLLPRWQQALYQHRTAKT
                     GLAQLQAQSAQSFYLVTLPALAFLTVCVILLFTFGFTMMFLDDFVFTGYWPWFYTHAT
                     MFALMMLGVNNAYWALMYLCLKQHLLLHGLQSPLP"
     gene            3930..4553
                     /locus_tag="PK856_05050"
     CDS             3930..4553
                     /locus_tag="PK856_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01769.1"
                     /translation="MKRQLLCLILAYFFIYVLVLGVYWAFKYAGGCAADWACVLDEDR
                     TIGILTLTSYMLTPALAIGGFISWKIQHNRGLLAEEAKHIFLAVNNDLKNLNQMEYLL
                     EHHNLKTDISDLSEQIQQTYQNLRNGNLDIYADALVLYEMSNDEKLAHIRHSYHDAGL
                     ALLNDLKKLKDSPITNSLQTQIEQTLAQKKDLNQPFKQVLRQYMIFK"
     gene            complement(4641..>5276)
                     /locus_tag="PK856_05055"
     CDS             complement(4641..>5276)
                     /locus_tag="PK856_05055"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRL01770.1"
                     /translation="GSLKKGKLTLGLPPLGSVLLSALIARFHAAYPDIQLIFLEVGAN
                     GIEQALADKSIDVGIVLGAQLRPGLAGISLIESPMMVLSHEHSAWRGRETIALSELQH
                     EPFLLYADTFTLNNLIVQAAHHAGFEPKVVCRSSQWEFMVKLVESNMGIALLPQVFAE
                     QVGHLHIHQATLIEPQMNWHLSMAWNTTVALSPAAQAWMAIIEEHKQHIHL"
BASE COUNT         1351 a         1499 c         1204 g         1222 t
ORIGIN      
        1 aaagacgcgc tgtccatgac caactcaccg cattttcgcg gctattcccg cgtcaatgag
       61 gaacacaccc gcaatcaacc cgattttcgt gagcaaatcg acatcggtgc cgatttggaa
      121 gccgttgaat ttgatgccaa cacaccgctg tggctgaagc tgcaaggccc gaatcaatgg
      181 cctgaaaact ggcctgaatt caaaaccatc gtcaccgctt ggcaaaccga tttgcgccgc
      241 attgccatca ccttattgcg agccttcaca ctggctttgg gtttgcctga agatgcattg
      301 gatcaatttg tcacaggcaa acccaatgaa ttattgaaac tgattcatta ccccagtgcc
      361 gagctcaacg aaggcaaaaa ccaaggcgtg ggcgcgcaca aagacgcagg cattttgacc
      421 ttgctcttgc aagaccaagt gggcggtttg caggtattga gcaaacaaga cggttggata
      481 gaagcccctt atgtggaagg cgctttcatc gtgaacatcg gtgaaatttt agaactggca
      541 accaatggct atttggtggc gaatatgcac caagtggtgt cgcccaaagc ccatgtcgat
      601 cgctattcca tcgcttattt catcaccccc aatttgtacg caggcgaggt gccgatttta
      661 gaattgcccg cagccttgca gcaactggcc acaggcccgc aaagcgatcc gctcaatcct
      721 ttgctcaaaa atgcaggtga aaacaccatc aaaggccgtt tgcgctcgca tttgaaagtc
      781 accgaaaaat tttaccccga agaataccaa cgcatactca accaacgcca acaacaagag
      841 gtgaccgcat gatacacgcc atcaaaaaat ccatcgccat tctgagccta ttggcagtgg
      901 tcgcttgctc caacagtgcc gacaaaccag ccgccagcac gcctgccgca gccaaaaccg
      961 acaccaccaa aatcgtcatt ggctctatgg gttcagactc agacatttgg cgcttcattg
     1021 ccaaatcgcc tgcggcccaa gaagcgggtt tggacatcga agtgaaagac atcaacgatg
     1081 gcatcacctt aaacaatgcc acagcagaag gccaagtcga tgtgaatgcc ttccaatcgt
     1141 gggcgtattt caaatcgttt aaccaaacca atggcgacaa attgggggct ttggccacca
     1201 cgtatttgga acccatgggt ttgtattcca aaacccacaa agacttgaac gccttacctc
     1261 aaggcgcgac cattgccatc cctaacgaca ccgccaacac ctcacgcgcc ttgcgcttgc
     1321 tggaagaagc gaaactcatc accctcaaag ccgatttcaa ccctgtcaca ggcacggtgc
     1381 aagacattgc cgacaacccg aaaaagctca acatcaaatt ggtgcaaggc gcttctggcc
     1441 cacgcgtgtt gagtgatgtg gatctggggg cgataggcaa taccaacgcc ttagaaagcg
     1501 gcctgaacgt caccaaagac gcgctgtttc atgagcaagt gggcgaccgc gtgctgaaca
     1561 acatcaatat tttggcgacc aaaaaagacc gtgtgaacga tgccgtattg caaaaactgt
     1621 cggcgctgta tcaccaacct gctgtcgatg cttacatcaa agagcatttc ggtggcacca
     1681 aactggatgt gaaccaaccg atttcttcat tgaattaatt caggcatatc caactctaag
     1741 cggcctgtaa accttacagg ccgcttaata gcctacaaaa tgccattgca accttacgaa
     1801 agcacacacc atgagcaaca caccgtaccg ccgcgatttg aatggcgaag acttaagccc
     1861 tgaaaccctg atgatgtctt acggctacga cccaaaattg tctgaaggct ccatcaagcc
     1921 accgattttc ctcacctcca ccttcgtgtt caaaagtgcc gaagaaggca aagatttttt
     1981 caacatcgtc agcggccgca cccctgcgcc tgccgagcaa aaaaatggct tggtgtattc
     2041 acgtttcaat cagcccaaca gcgaaatcgt tgaagaccgc ctcgccttgt acgaacgcgg
     2101 tgaagacgcg gctttgtttg cctctggcat ggccgccatc accaccagct tgttggcttt
     2161 tgtgcagcct ggcgatgtga ttttgcaatc gcagcctttg tacggcggca ccgaaaccct
     2221 gttgttgggc ttgttcaaac gcttgggcat agacagcatc cattttgaca acggctgcga
     2281 tgcctattct gtgcgcgccg ccatcgcgca agtgcccgca ggtcgtcgca tcgtcgccat
     2341 ttacgtggaa acaccatcca accccaccaa caccatagtt gacatcgcct tattggccaa
     2401 cattgcccaa gaactggccc aacgacaaaa cggcgacaaa cccctgctga tttgcgacaa
     2461 caccttgctc ggccctgtgt ttcaacgccc gctcgaacac ggtgcagact tgtcggtgta
     2521 ttccttaacc aaatacgtcg gcggccattc cgatgtggtg gcaggtgcgg ccatcggcag
     2581 caaagcctta ttgaaaaaaa tcaaaagcca acgcagcgcc atcggcaccc agctcgaccc
     2641 acattcctgc tggatgctcg gccgctcact ggaaaccttg gcattgcgca tggacaaagc
     2701 caatgccaat gccgcgcaat tggcacaatt cttacacgcc catcccaaaa tcaaacgtgt
     2761 gcaccatttg gatttcctcg ctgaaaacag ccctgaatac agcgtacacc aaagccaaag
     2821 cagcggcgca ggctccacgt ttgccatcga agtgggcgac gatgaagcgg cagcgtttcg
     2881 ctttttgaac catttggcca ttttcaaatt ggccgtgagc ttgggcggca cggaatccct
     2941 cgccagccac cccgcttcca ccacccactc aggcatcgcg gcagacgtgc gcgcgcgcat
     3001 cggcataggc gacaatttgg tgcgcttgtc cataggcatc gaaaacgcca acgatttgca
     3061 acgcgccttg agtttggctt tggaagcggt gtaagcccaa ccccatcacg actatccaaa
     3121 taagcggcct gtaacaaccc aaatggtttt acaggccgct tgcttgtgtt gcttcaatgc
     3181 ccttctcttg ctggcatgat tcgtgtttat actgtgccca gtacaccacc ataaattcac
     3241 catgcccaac gccttgcgcc aaaatttgtt tttattgccc gcctctgccc actcttcact
     3301 gctgcgcatg agttggcggc cgcttgtcgc ttgttacggc ttcatgctgc tggccagcat
     3361 gattgggctg ggtgtggatg ccatgcatca tcccgattta caacacatgg cttatgactc
     3421 ttggcaattg tcttggcgac attcgcaatg ggcagtggct tgcctgctgt ttttaatggt
     3481 gtttgtgcga cccaaactgc tgccacgttg gcaacaggcg ctgtaccaac accgcaccgc
     3541 caaaactggt ttagcgcaat tgcaagcgca atctgcccaa tccttttatt tggtcaccct
     3601 acctgccttg gcttttctca ccgtctgcgt catcttattg ttcacctttg gttttaccat
     3661 gatgttcttg gatgattttg tgttcacagg ttattggcca tggttttata cccatgccac
     3721 gatgtttgca ctgatgatgc tgggcgtgaa caatgcctat tgggcactga tgtacctgtg
     3781 tttgaaacaa cacttattgc tacatggctt acagtcgccg ctgccatgaa acatgtggcc
     3841 tgtaaaatcg cctatgatga atgccaaaca tttgaaacaa gctttagacg actgcctctc
     3901 accgccccaa ccacccacac ggactgacca tgaaacgaca attgttgtgt ttgattttgg
     3961 cttatttttt catctatgtt ttggtattgg gtgtctactg ggccttcaaa tatgcaggtg
     4021 gctgcgccgc agattgggca tgtgtgttgg atgaagaccg caccataggc atcctcaccc
     4081 tcacttccta catgctcacc cctgctctgg cgataggcgg cttcatcagt tggaaaatcc
     4141 agcacaatcg tggcttgctg gctgaggagg ccaaacacat ttttttggcc gtcaacaacg
     4201 atttgaaaaa cctcaatcaa atggaatacc tgctggaaca ccacaacctg aaaaccgaca
     4261 tcagcgattt gtcagaacaa atccagcaaa cctaccaaaa tttgcgcaat ggcaatctgg
     4321 acatttacgc cgatgccttg gttttgtatg aaatgtccaa cgatgaaaag cttgctcaca
     4381 tccgccacag ctaccacgat gctggcttgg ccttgctcaa tgacttaaaa aaactcaaag
     4441 acagccccat caccaacagt ttgcaaaccc aaattgaaca aactttggcg caaaaaaaag
     4501 acttgaatca gccgttcaaa caggtattgc gtcagtacat gatttttaaa taaaccatgc
     4561 ctcgcatgca tcgcaacacc cacaagcggc ctgttaggct ctaatgcgtt tacaggccgc
     4621 ttaatattgc gaggcgaaca ctacaaatga atgtgttgct tgtgttcttc aatgatggcc
     4681 atccaagctt gcgcggcagg gctcaaggcc acggtggtgt tccacgccat gctcaaatgc
     4741 caattcattt gcggctcgat aagcgtggct tgatggatgt gcaaatgccc cacttgctcg
     4801 gcaaacactt gcggtaacaa ggcaatgccc atattggatt ccaccagttt caccataaat
     4861 tcccactggc tagacctaca caccaccttg ggctcaaagc ccgcatggtg agcggcctgt
     4921 actatcaggt tgtttaaggt gaaggtatcg gcatacagca aaaacggctc gtgttgcaat
     4981 tcactcaagg cgatggtttc gcgcccgcgc caagccgaat gttcgtgtga caacaccatc
     5041 atcggcgact caataaggct gatgcctgcc aagccaggcc gcagttgcgc accgagcaca
     5101 atgcccacat cgatgctttt gtcggccaaa gcctgttcta tgccattggc gcccacttcc
     5161 aaaaaaatca gctgtatgtc gggataggca gcgtgaaagc gcgcaatcag cgcgctgagc
     5221 aataccgaac ccaaaggcgg caagcccaag gtcagcttgc cttttttcaa gctgcc
//
LOCUS       DAOMVK010000110         5261 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_4351_length_5261_cov_2.896658, whole genome shotgun sequence.
ACCESSION   DAOMVK010000110 DAOMVK010000000
VERSION     DAOMVK010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5261
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4351_length_5261_cov_2.896658"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..130)
                     /locus_tag="PK856_05060"
     CDS             complement(<1..130)
                     /locus_tag="PK856_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRL01771.1"
                     /translation="MRCFEAAARHLSFTLAAEELNMTQSAVSKQVAMLEYTLKIQLF"
     gene            422..883
                     /locus_tag="PK856_05065"
     CDS             422..883
                     /locus_tag="PK856_05065"
                     /inference="COORDINATES: protein motif:HMM:NF023204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HRL01772.1"
                     /translation="MKKTITAAIVASLLGMMTMPALADNGRRGHDDYKHIQKSYKEQQ
                     KSYRKYQKDRYKYEREHNRYEHNRYNDRYYERNRYEPHYRPPVYSSQRVWRRGEVLDR
                     SYRGQGYWVNDYRQYPRLYAPAKNQRWVRVDNDYILVNTLSNVIVSILTGR"
     gene            complement(962..1399)
                     /locus_tag="PK856_05070"
     CDS             complement(962..1399)
                     /locus_tag="PK856_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01773.1"
                     /translation="MDKAYGLGMVLAGLAAWASAQNYNNGEDLAKFVTSETFAQMKAS
                     ELKPIRDKVGKHLHQCVNATIVDLRKNPPKEGVNAAQLQRYYQAWIPFQKAGCEIYAT
                     EQEGTASDRADAQMRCEIFTQSSLYSDVLGYQSQALPVCTAAE"
     gene            complement(1412..1798)
                     /locus_tag="PK856_05075"
     CDS             complement(1412..1798)
                     /locus_tag="PK856_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01774.1"
                     /translation="MKKTLCFVIQLSHNRLSVSWGSHENVHIQADFVHPRTVLGRYEV
                     LVQALKPIVTQAKSTQEYGRWWQRTPIILWLLPPQIGGVHELERHCLSELGFEICNGR
                     CIAYVFESEAMSYAQAQAIIPTLKQI"
     gene            1912..2571
                     /locus_tag="PK856_05080"
     CDS             1912..2571
                     /locus_tag="PK856_05080"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRL01775.1"
                     /translation="MAQLSQLQTIESQLSCPHGDAGIDFAHMMHHTNVGMTQAGFTAL
                     KLPFSAHLLEIGHGNAAHLPLLQQIAGLHYTGLEVSSLMQQEAMRLNQAAVAAHMAEF
                     LHYDGNTLPQFSQLFDGILCVNTVYFWAQPTAFLQALRSLLKPTGRLSLVYMDEDYMR
                     SLPFVGKRFQVYSVSVLQDLVCQAGFVLLETQDRQDQVAMKDTGKLIDRHYHVSCFGL
                     A"
     gene            complement(2943..3125)
                     /locus_tag="PK856_05085"
     CDS             complement(2943..3125)
                     /locus_tag="PK856_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01776.1"
                     /translation="MKKEKTGKKDSQLLIRISSSERDAFIQLCDELDTSAARELRKFI
                     RSFIKKHQPDESENKE"
     gene            complement(3248..4144)
                     /locus_tag="PK856_05090"
     CDS             complement(3248..4144)
                     /locus_tag="PK856_05090"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRL01777.1"
                     /translation="MKFDLTDLQLFLNIVDAGSITAGAERSHLALASASERLRKMEQS
                     LGTGLLVRLPRGIEVTAAGAALVHHARLMLQQQHRLKTELSGFVQASKSHVCLYANSA
                     ALTEFLPSKLAAWLAENPQVRLELKERTSADIVHAIAAGLADVGMVSNAVNSKGLSMH
                     PVATDDLVVLVSQSHRCANQKKVSFEDILTEPFVGLSEGSALQKYIAQQAEMLGGKLD
                     VRVHMNTFAGVCEMVAHDVGVGMVPWSAAKRYRRKYPFHLLSLTDDWAHRQLCACFRN
                     WDELSVPIKSLLLGLGVDENGV"
     gene            4258..5004
                     /locus_tag="PK856_05095"
     CDS             4258..5004
                     /locus_tag="PK856_05095"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRL01778.1"
                     /translation="MSDPTLFAILIVLTFLTAGTVKGITGMGLPTVAMGILGAFTSPT
                     MAAALLLIPSLVTNVWQMLAGKSLAMLVHRFGLMSVCVVIGTVMGAQLLQDISPLWAG
                     FGLGVALIVYAGYSLFAPNLSIPLTWESKLSPLIGMVTGFITGLTGVFVMPAVPYLSA
                     LKMDKETLVQALGLSFTVSTVALGLGLFHQQAFNLSQLQASAWAVVPALLGMWLGQHI
                     RQRISPKRFRVCFLMFLLMLGLQLLLKPWY"
BASE COUNT         1332 a         1415 c         1113 g         1401 t
ORIGIN      
        1 tgaacaattg gatttttaag gtgtattcca acatcgccac ttgcttgctc acggcgcttt
       61 gggtcatgtt caattcttcg gccgccagcg tgaagctcaa gtgtctggcg gcggcttcaa
      121 aacagcgcat ggcatgggta gacggaattt gtttgcggta aagcttatta ttcattgtgt
      181 gtaggccaat caactgatgt gtctttgtag cggtaggctt gaatatagcc tgatttggca
      241 ggcaagagaa caatctggtg ctcaaaaccc acccaatccc acacatctcc tgacatacct
      301 tgctacaaat taaccatagc ttacctatct ttagattcca ctaagcatct actaagtttc
      361 gcgatttacc atgctcacat aaacccagat ttgtttgagt acacactctt aggagacagt
      421 catgaaaaaa accattaccg ctgccatcgt ggcgagttta ttaggcatga tgaccatgcc
      481 cgccttggcc gacaatggtc gccgtggaca tgatgattac aaacacatcc aaaaatcgta
      541 caaagaacag caaaaatcgt atcgtaaata ccaaaaagac cgttacaaat acgagcgtga
      601 acacaatcga tatgagcaca atcgctataa cgaccgctat tacgaacgca accgctacga
      661 accccattat cgtccacccg tatactcaag ccaacgtgtt tggcgtcgtg gtgaagtgtt
      721 agaccgctct taccgtggtc aaggctattg ggtcaacgat taccgccaat atccgcgctt
      781 gtatgcgcca gccaaaaacc aacgctgggt gcgcgttgac aatgattaca tcttggtcaa
      841 caccttaagc aatgtcatcg tcagcatctt aacgggccgt taagcccgtt ggtatccaaa
      901 cacttacagg ccgcatatgc ggcctgtaag catcaatagc atcatcttga ggcaatcttg
      961 cttattctgc cgccgtacat actggcagtg cttggctttg atagcccaat acgtcgctgt
     1021 acaaagacga ttgggtaaaa atctcacaac gcatttgtgc atctgcacgg tcgctggcag
     1081 tgccctcttg ttcggttgca tagatttcac aaccggcttt ttgaaaagga atccacgctt
     1141 ggtaatagcg ttgcaattgc gccgcattca ccccttcttt gggtggattt tttcgcaaat
     1201 ccactatggt tgcgttcaca cattggtgta aatgtttgcc cactttatcg cgtatcggct
     1261 tgagttcact ggctttcatc tgggcgaatg tctcactggt cacgaatttg gccaaatcct
     1321 cgccattatt gtagttttgc gcgcttgccc atgccgccaa tcccgccaac accatcccca
     1381 aaccatacgc cttgtccata cacatccctt gttaaatctg cttcaaagtc ggaattatag
     1441 cttgagcttg ggcataactc atcgcttcgc tttcaaaaac atacgcaatg cagcgaccat
     1501 tgcaaatttc aaagcccagt tcgctcaagc aatgccgttc caactcatgc actccgccga
     1561 tttgcggcgg caataaccac aaaattatcg gtgttctctg ccaccaacgc ccgtattcct
     1621 gcgtgctttt cgcctgcgtc acaatgggtt tcagtgcctg taccaacacc tcataccgtc
     1681 ccaataccgt gcgaggatgg acaaagtccg cttgaatgtg aacattttca tgactccccc
     1741 aactcacact taagcgattg tgagagagtt gaatcacaaa acataaggtc tttttcatgg
     1801 cagtgtcacg cagtatgaga tgccctcatc ttagcccaat cgggtttacg tctgcacaat
     1861 taacatggca tattgtcagc atctaccatc atgtcaccca caaggaggaa tatggcacag
     1921 ctgtcacaat tgcaaaccat agaaagtcaa ttgtcttgcc cacatggcga tgcgggcatt
     1981 gattttgccc acatgatgca ccataccaac gtgggcatga cccaagcagg attcaccgct
     2041 ttgaagctgc ccttctcagc ccatttgctg gaaatcggcc acggcaatgc cgcccacctg
     2101 cccttgttgc aacaaatcgc agggctgcat tacacaggtt tggaagtatc aagcttgatg
     2161 caacaagaag ccatgcgtct gaaccaagcg gctgtggccg cgcacatggc cgaatttctg
     2221 cattacgatg gcaacacact ccctcaattc tcacagctgt ttgatggtat tttgtgcgtg
     2281 aacaccgtgt atttttgggc acaacctacc gcatttttac aagcactgcg ctcgctgctc
     2341 aagcctacag gccgcttgag cttggtttac atggatgaag actacatgcg ctctttaccg
     2401 tttgtgggta aacggtttca agtgtattca gtgagcgtcc tgcaagactt ggtctgccaa
     2461 gcaggtttcg tcttgttaga aacccaagac cgccaagacc aagttgccat gaaagacaca
     2521 ggcaaactca tagacagaca ctaccatgtg tcatgtttcg gtttggctta agagaggctc
     2581 aaccttaagc tcatgcggcc tgtaaacacg gtatggattg cttcagtcat ggattcctgt
     2641 ggttaaagct gagtgtttca agcagcatgc tatttgtcac ttacgcccaa accagtgctg
     2701 attcacttaa aacggtgact aagccaaatc gccatgcaaa tcacccgctg tacacagcgg
     2761 gtgatattga ttctggcttc aggcaaaaaa ctctgtccct aagcccctag cgctaagcct
     2821 gttatttcgc ggcttttttc agttttttct ttaatttgcc caattttgat tcgtgtttgg
     2881 ccactttttt ggcacgggct ttaatggctt tttccaattt tttgatttgc tttgacatgg
     2941 tgtcactcct tgttttcaga ttcatcaggt tggtgctttt taataaagct gcggataaac
     3001 tttctaagct ctcgtgctgc actggtgtcc aattcatcac acaattggat gaaagcatca
     3061 cgctcgctgc tgctgattcg tatcaagagc tggctgtctt ttttgccagt cttttctttt
     3121 ttcacgtcgc cactcatttt aaacctcact cagaaaagac catctcgaca aaatgtatat
     3181 acataatata tacaaaaatc acacctgtca aattgcaaaa gtaatgtacg aacgaaatcc
     3241 actggtttca aaccccgttt tcatccacac ccaagccaag taacagactt ttgatgggaa
     3301 cagaaagctc atcccaattc ctgaagcaag cacacagttg gcgatgtgcc caatcatcgg
     3361 tcaaagacaa caaatggaat gggtatttac gccgataacg cttggccgca ctccatggca
     3421 ccatccccac acccacatca tgcgccacca tttcacacac cccagcaaaa gtattcatgt
     3481 gtacgcgcac atccaatttc ccgcctaaca tctcagcctg ctgcgcaatg tatttttgca
     3541 aagcactgcc ctcagacaaa cccacaaatg gctcggtcaa tatatcctca aaacttacct
     3601 ttttctggtt cgcgcatcgg tgcgattgcg aaaccaacac caccaaatca tccgtggcca
     3661 caggatgcat ggacaatcct tttgaattga ccgcattgga aaccatgccc acatcagcca
     3721 aacccgcagc aatggcatgc actatgtccg cactggtgcg ctctttgagc tccaaacgca
     3781 cttgtgggtt ttctgccagc caagccgcta gtttgcttgg cagaaactca gtcaatgccg
     3841 ccgaattggc atacaaacac acatggcttt ttgatgcttg cacaaaaccg ctcaattcgg
     3901 ttttaaggcg atgctgttgc tgcaacatca agcgagcatg gtggaccaaa gcggcgcccg
     3961 ccgcagtcac ttctatccct cttggcagcc tcaccaacaa acctgtgccc aaagactgct
     4021 ccattttgcg caatcgctcg ctggctgaag ccaatgccaa atgagaacgc tcggcacctg
     4081 cggtgatgct gccagcatcc acaatgttca aaaacaactg taagtcggtt aaatcaaatt
     4141 tcatgtctgt gagcccgccc aagcttcgcc aaaaccgaag gatgatatgg aaatgccaca
     4201 ttgttccaca tccacacaaa atgcttcaat actgttttca attcagagac ttgcaccatg
     4261 tccgacccca ctttatttgc catcctcatt gttctgactt ttttaacagc tggcacagta
     4321 aaaggcatca ctggcatggg tttgcctacc gtggccatgg gcatactggg cgcattcacg
     4381 tcccccacca tggcagctgc cctgctcctc attccgtcct tggtcaccaa tgtctggcaa
     4441 atgctggcag gcaagtcttt ggccatgttg gttcaccgct ttggactcat gtcggtgtgt
     4501 gtggtcatcg gcacagtaat gggcgcgcaa ttattgcaag acatttcacc cttgtgggca
     4561 ggctttggtc ttggcgtggc gttaattgtg tatgcaggct attccttgtt tgctcccaat
     4621 ctctcgattc cactgacgtg ggaaagcaaa ctctcaccac tcattggcat ggtgacaggg
     4681 ttcatcacag gactcacagg cgtgtttgta atgcctgccg tgccttattt gagcgcctta
     4741 aaaatggaca aagagacctt ggtgcaggct ttgggtttgt cgtttactgt ttcaactgtt
     4801 gcactgggat tgggtttgtt tcaccaacaa gcgttcaatc taagccaatt gcaagcttcc
     4861 gcttgggcag ttgtgcccgc tttactgggc atgtggctgg gccaacacat acgccaacgc
     4921 atcagcccaa aacgttttag ggtgtgcttt ttaatgttct tattgatgtt aggcttgcag
     4981 ctgctgctga agccttggta ttgaatagcg agtgtgttaa caacacttca tgcaactgat
     5041 aactgaatct gacaataccc ataagcggcc tgtaaactct ttacaggccg cttgttaccc
     5101 cattcaatgc tcacgatgcg gtgtgcgcac aaacaccacc gctgccatca tccacagcaa
     5161 cagacctaac caaaacaacc agcgtggcca cgcagcagcg gcatgttgta cctcaaaccc
     5221 cgttacgtac agaatttcgc aagcgccatt gcctgtgtca c
//
LOCUS       DAOMVK010000111         5205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_4423_length_5205_cov_2.758641, whole genome shotgun sequence.
ACCESSION   DAOMVK010000111 DAOMVK010000000
VERSION     DAOMVK010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4423_length_5205_cov_2.758641"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="PK856_05100"
     CDS             <1..928
                     /locus_tag="PK856_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HRL01779.1"
                     /translation="EALATQIKTFDEHIGIGQEQLKQLQQQRQHLQQQQQQLEQLLPQ
                     WRAQLQQQLLNAQQAQMNKQRYGEQLQERGANWTALQEIADVDAGKLQQQISSIAKQL
                     QAMGAVNLAALQELSEARERFDYYQNQRNDVQQAMDALHEAIAQIDNETKTLFQQTFD
                     AVNTHMQEFFPTLFGGGEAHLQLIGDDMLDAGVAIMARPPGKKNSTIHLLSGGEKALT
                     AMSLVFALFSLNPAPFCLLDEVDAPLDDANTSRFCRLVEQMSQHTQFLYISHNRLTME
                     MAEQLIGVTMQEKGVSRIVEVDIKQALELAAQ"
     gene            1050..1721
                     /gene="tenA"
                     /locus_tag="PK856_05105"
     CDS             1050..1721
                     /gene="tenA"
                     /locus_tag="PK856_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HRL01780.1"
                     /translation="MSFSQEVWARNADLYQATLQLPFNQELAAGTLSQEAFVHYVIQD
                     AHYLLAYGRALAVIGAKADHADSVIQFTEAAKLAIIVERSLHDGFMADFGITKTQFET
                     TPLSLACHHYVSFLQATAWAESYPVALAAVLPCYWVYAQVGNDIVGKSAPNNPYQKWV
                     DTYVSDEFQQAVEKVLQTIDKLAENADANTLEKMHAAYTRGAELEWLFWDSAYKRETW
                     PQMTK"
     gene            1735..2136
                     /locus_tag="PK856_05110"
     CDS             1735..2136
                     /locus_tag="PK856_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01781.1"
                     /translation="MQEQLQHTRQQWLQAYYDGDVLQLAQHEHPQFQLHNRLSGHIEG
                     RERHEHIAFAVAHQAWKPARWHIACERFAFSDDGLQCQVATEADTPAVSIQEFWMWEQ
                     CWRIVQCVLEPVPENTAIIPTSDHTGLTGCL"
     gene            2133..3032
                     /locus_tag="PK856_05115"
     CDS             2133..3032
                     /locus_tag="PK856_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01782.1"
                     /translation="MKTKSQPDILQAWLKTQDLPPTDHLYMKDAAANDNEVFKMVMGN
                     HDWMVVTPSKAKNGWGKILNMRMVKTWSWAELCDSRIEPQNKPLQELPFQQNLTWRDG
                     GSILLSMVLVFSAFYLWIRFFSATMDLYVWLMGFYFSLILYQGSAKWQKVRAQTKPPT
                     PWRSRLMALQQSLLLQTIVCLGMGFVCARWLFMPMSQQLGSLFGIQGDPAFKTMLTIY
                     LPLTWSYWWFNCRLDARLNTEEHAHHYELWLYHRNGSRTCYARANEHASLELLQNWLQ
                     AQQQRYFDAHPAHITHTNLTGRL"
     gene            3029..3916
                     /locus_tag="PK856_05120"
     CDS             3029..3916
                     /locus_tag="PK856_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01783.1"
                     /translation="MKAKSQPDVLQTWLATQDLPPLDRQYMKYAAANDNEVFKMVMGN
                     HDWMVVTPSEAKSGWGKILNMRMVKTWSFAELCDSRIEPQNKPLQELPFQKKLTWRDG
                     VDIVLMLLLFIAFIFVVGFFKPLELYVWFFGLYCCLSVFSIGAKWCRVRAQFAPLKLW
                     QSWFISTAESALLRGVLSLSLGYVLALGLFLPLIQLFELHFGPKTGIALVFGSYLVFA
                     CVDRWLNRRLDARLNTTELAQHYELWLYNRDGSRTCYARANERTSLELLQHWLKAQQQ
                     RYFDAHPAQQTHTQLTGRL"
     gene            3913..4725
                     /locus_tag="PK856_05125"
     CDS             3913..4725
                     /locus_tag="PK856_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01784.1"
                     /translation="MKASNDLLAVWRQQQQLGSSDDEILRNHACDGAVWYKKPEQYIA
                     RLAQIHTTKITSAQSTPPTTPTLSIVRMMLLLGMMLGSPWLSPWLNRVLSVPQLLLLL
                     VAVMLLFFCLLLYPYFAHPRRSHRHGYQLWFYPSTGAPLLAITLSDLEALQALQAWLH
                     SHMPPSSMCENRLNADVIQDWLAEQQPSLMNYELLADLRAHKQVVYWGQQELLLLKFT
                     QSLHIHKVEGRYQPPALNRHSDAMAQLWFMVLLGLYFYLPETPVPNLWLWAH"
     gene            4752..4970
                     /locus_tag="PK856_05130"
     CDS             4752..4970
                     /locus_tag="PK856_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01785.1"
                     /translation="MLILPASSLSDAFSRGKTNHPSGPNAHYLLLATTSLDPMDVQVA
                     ASYYFPLLMGLRNLLLQQQNPSQSLPNE"
BASE COUNT         1325 a         1370 c         1250 g         1260 t
ORIGIN      
        1 tgaagccttg gccacccaaa tcaaaacctt cgacgaacac ataggcatag gccaagagca
       61 gctgaaacaa ctgcaacaac agcgccaaca cttacagcaa caacagcaac agctggaaca
      121 attgttgcca caatggcgcg cgcaattgca gcaacaattg ctcaatgccc aacaagcgca
      181 gatgaacaag cagcgctatg gcgaacaatt gcaagagcgt ggtgccaatt ggacggcgct
      241 gcaagaaatc gccgatgtgg acgcgggcaa attacaacag caaatttcca gcatcgccaa
      301 gcaattgcaa gccatgggcg cggtgaattt agccgctttg caagaattga gcgaagcgcg
      361 agagcgtttt gattattacc aaaaccagcg caacgatgta cagcaagcaa tggacgcgtt
      421 gcatgaagcg attgcacaaa tcgacaacga aaccaaaacc ctgtttcaac agacgtttga
      481 tgcggtcaat acccacatgc aagaattctt cccgaccttg tttggcggcg gcgaagcaca
      541 tttgcaatta atcggtgatg acatgctcga tgcaggcgtg gccatcatgg cgcgcccacc
      601 tggcaagaaa aattccacca tacatctgct ctcaggcggt gaaaaagcgc tgacggcgat
      661 gtcattggtg tttgccttgt tcagcctcaa tcctgcgccg ttttgtttgc tcgatgaagt
      721 ggatgcgccg ctcgatgatg ccaacacctc acgcttctgc cgcttggtcg agcaaatgag
      781 ccaacacacg caatttttgt acatctcgca caaccgcctc accatggaaa tggccgagca
      841 gctgatcggc gtgaccatgc aagaaaaagg cgtgtcacgc atcgtggaag tggacatcaa
      901 acaagccttg gaattggcgg cgcaataagc ctgattgccc ttggcatggc ttaagccgcc
      961 catatctgtt ataaacccta ttcccttaaa caagcggcct gtaaagctta caggccgctt
     1021 ttgttgtcac actcatcaag gaagccacca tgtcgttttc tcaagaagtt tgggcacgca
     1081 atgccgattt gtaccaagcc actttgcaat tgccgtttaa ccaagaattg gccgcaggca
     1141 cgctcagcca agaagcgttt gtgcattatg tgatacaaga tgcacactat ttgctcgctt
     1201 atggccgcgc tttggccgtt atcggtgcca aggccgacca tgcagactcg gtgattcaat
     1261 tcactgaggc ggccaaactg gccatcatcg tggaacgcag cttgcatgat ggctttatgg
     1321 ccgattttgg catcaccaaa acccagtttg aaaccacgcc attgagtttg gcttgccacc
     1381 attacgtgag cttcttgcaa gccactgctt gggcagaaag ctatcctgtg gccttggccg
     1441 ccgtattgcc ttgctattgg gtgtatgccc aagtgggcaa cgacattgtg ggcaaatctg
     1501 ctcccaacaa tccctaccaa aaatgggtgg atacctacgt cagcgacgaa ttccagcaag
     1561 cagtggaaaa agtgttgcaa accatcgata aactggctga gaatgctgat gcaaacactc
     1621 tagaaaaaat gcatgcggcg tatacgcgcg gggcagaatt ggaatggctg ttctgggatt
     1681 cggcttacaa acgcgagaca tggccgcaga tgaccaaatg attaagggat aaacatgcaa
     1741 gaacaattgc agcacaccag acaacaatgg ctacaggcct attacgatgg agatgtgctg
     1801 caattggcac agcatgaaca cccacaattt caactacaca accgcttaag cggccacatc
     1861 gaaggccgcg agcgccatga gcacatcgca tttgccgtgg cgcaccaagc ttggaaaccc
     1921 gctcgttggc acatcgcctg cgagcgtttt gcgttttctg acgatggttt gcaatgccaa
     1981 gtagccaccg aagctgatac tcctgcggtg tccatccaag aattttggat gtgggagcag
     2041 tgttggcgca ttgttcaatg cgtgttagag cctgtgcctg aaaatacagc catcatacct
     2101 acatctgacc acacgggtct tacaggctgc ttatgaaaac caagtctcaa cctgatatct
     2161 tacaagcttg gctgaagaca caagacttgc cacccacaga ccacctgtac atgaaagacg
     2221 cggctgccaa cgacaacgaa gtgttcaaaa tggtgatggg caaccatgat tggatggtgg
     2281 tcacacccag caaagccaaa aatggctggg gcaaaattct gaacatgcgc atggtcaaaa
     2341 cctggtcttg ggccgagctg tgtgacagcc gcattgagcc acaaaacaaa ccactgcaag
     2401 aattaccgtt tcaacaaaac ctgacttggc gcgatggcgg cagcattttg ctgagtatgg
     2461 tattggtctt cagtgctttt tacctgtgga tacggttttt ttcagccacc atggatcttt
     2521 atgtttggct tatgggtttt tatttcagcc ttattctgta tcagggcagt gccaaatggc
     2581 aaaaagtgcg tgcccaaacc aagccaccca caccgtggcg ctcacgactg atggccttgc
     2641 agcaatcgct gttgctgcaa accatcgtct gtttgggcat gggctttgtt tgcgcgcgtt
     2701 ggttgttcat gcctatgtca caacaattgg gcagcctctt cggaattcag ggtgatcccg
     2761 cttttaaaac catgctgacc atctacttgc cacttacttg gtcatattgg tggttcaact
     2821 gccgtttgga tgcgcgcttg aataccgaag aacatgccca ccattacgag ttatggctgt
     2881 accaccgcaa tggttcacgt acttgctacg cccgcgccaa tgaacacgcc agcttggaat
     2941 tgttacagaa ctggctacaa gcgcagcaac agcgctattt cgatgcccac cccgcccaca
     3001 tcacccacac taatcttaca ggccgcttat gaaagccaag tcccaacctg atgtgttaca
     3061 aacttggctg gccacgcaag acttgccgcc tttagaccgt caatacatga agtatgctgc
     3121 agccaatgac aacgaagtgt tcaaaatggt gatgggcaat cacgattgga tggtggtcac
     3181 acccagcgaa gccaaaagtg gttggggcaa aatcttgaac atgcgcatgg tcaagacttg
     3241 gtcttttgcc gagttgtgcg acagccgcat agaaccacaa aacaaaccac tgcaagaatt
     3301 accgtttcaa aaaaagctga cttggcgcga tggtgttgac attgtgttga tgctgctttt
     3361 gtttatcgct tttatattcg tggttggatt tttcaaacca ctggagcttt atgtgtggtt
     3421 cttcggctta tactgctgct tgtcggtgtt cagcattggt gccaaatggt gccgtgttcg
     3481 cgcccaattc gcgccgctta aactttggca atcttggttt atcagcacag cagaatcggc
     3541 gcttctgcgc ggggtcttga gtttaagctt gggctatgtt cttgcgcttg gcttattcct
     3601 ccccttgata caattgtttg aactccactt tgggcccaaa actggaattg cattggtatt
     3661 tggcagctat ctggtcttcg cttgcgttga cagatggctg aatcgccgct tggatgcacg
     3721 tttaaacaca actgagcttg cccagcatta cgaattgtgg ctgtacaacc gcgatggctc
     3781 gcgcacttgc tacgcccgtg ccaatgagcg caccagtttg gaattgttgc aacactggct
     3841 caaagcacag caacagcggt atttcgatgc ccatcctgcc cagcaaaccc atacccaact
     3901 tacaggccgc ttatgaaagc cagtaatgat ttattggccg tgtggcgaca acaacagcag
     3961 cttggcagca gcgatgatga aatcttgcgc aaccatgctt gcgacggcgc agtgtggtat
     4021 aaaaaacccg agcaatacat tgcccgcttg gcgcagatac acaccaccaa aatcaccagt
     4081 gcgcagtcca caccaccgac cacacctaca ctctccattg tgaggatgat gcttttactg
     4141 ggcatgatgt tgggctcgcc atggttgtcg ccttggctca atcgcgtttt gtcagtgccg
     4201 caacttttgc tgctgttggt ggcggtgatg ctgctgtttt tttgtttgct gctttaccct
     4261 tattttgcgc acccccgtcg cagccaccgc catggctatc aattgtggtt ttacccatcc
     4321 acaggtgcgc cgctgttggc catcactctc agcgatttag aagcgctaca agccttacaa
     4381 gcatggctac acagccacat gccaccgagc agcatgtgtg aaaacaggct caatgcggat
     4441 gtgatacaag attggttggc cgagcaacag ccctcgttga tgaattatga attgctggca
     4501 gatttaaggg cgcacaagca agtggtgtat tggggccagc aagaattact gttgctcaaa
     4561 ttcacccagt ctttgcacat acacaaagtc gagggccgat atcaaccgcc cgcactgaac
     4621 agacactctg atgccatggc tcagctttgg tttatggtgt tgttaggctt gtatttttac
     4681 ctgcctgaaa cccctgttcc caacctctgg ctgtgggctc attagtcgtc ttactcacgg
     4741 catggttgat cttgctgatt ttgcctgcct cttctctttc ggatgctttc tctcgcggca
     4801 aaacaaacca cccttcaggg ccaaacgccc attatttgct gctggcaacg acctcattgg
     4861 accccatgga tgtgcaggtt gccgcctctt actatttccc cttgctcatg ggcttgcgca
     4921 acttattgtt gcagcagcaa aatccatcgc agtctctgcc aaatgaataa ggttcttggc
     4981 attgcagcca cagatgtgtg aagattcgag agctttttaa agatgggatg agggccataa
     5041 actcatatat aagcgacctg taaacccatc acaccacctc aaaaccatac aaatcgacaa
     5101 aggaaagcca tgagcttgat acaactggac atccaccaac acgtggccac cgttaccctc
     5161 aatcggcctg aacaaggcaa cagcatcgat ttggccagtg ccaaa
//
LOCUS       DAOMVK010000112         5159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_4478_length_5159_cov_2.172806, whole genome shotgun sequence.
ACCESSION   DAOMVK010000112 DAOMVK010000000
VERSION     DAOMVK010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4478_length_5159_cov_2.172806"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1455
                     /gene="lpdA"
                     /locus_tag="PK856_05135"
     CDS             <1..1455
                     /gene="lpdA"
                     /locus_tag="PK856_05135"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRL01786.1"
                     /translation="ASFAGSADAEYDVVVLGGGPGGYSAAFAAADQGLKTAIIERYAT
                     LGGVCLNVGCIPSKALLHNAAVIDEVKHLAANGIKFAEPEIDIDMLRGYKEKVIGKLT
                     GGLAGMAKARKVDVIRGNAQFVSANHFEVAETTGTGSEETGSKKTVAFKNAIIAAGSR
                     VTKLPFIPEDPRIVDSTGALELKGVPEKMLIIGGGIIGLEMGTVYSTLGARLDVVEMM
                     DGLMQGADRDLVKVWQKMNEYRFDNIMLNTKTVGVEAKEDGIYVTFEGAKAPTEPQRY
                     DLVLVAAGRQPNGLLIGADKAGVAVTDRGFINVDKQQRTNVPHIYAIGDIVGQPMLAH
                     KAVHEGHVAAENCAGHKAFFDARVIPGVAYTDPEVAWVGVTEEIAKRDGIKITKSVFP
                     WAASGRAIANGRDEGMTKLIFDAETGLIIGGGIVGTHAGDMIGEVCLAIEMGCDAEDI
                     GKTIHPHPTLGESIGMAAEVALGVCTDLPPMRKK"
     gene            1533..2849
                     /locus_tag="PK856_05140"
     CDS             1533..2849
                     /locus_tag="PK856_05140"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL01787.1"
                     /translation="MQHHNVNADVLPARRGLARQDSKVLALASLGGALEFYDFMIFAL
                     MAPILSQLFFPTSLGDGWALFYTYAIFATGYFMRPVGGLVMAHLGDRLGRKRMFTWSL
                     LLMALPTLAMGLLPTYAQIGLVAPLLLLLCRMVQAVAIGGEVPAAWTYVSEQVPVNQV
                     GLANGLLTAGLSVGILLGSLSVLALQAAFSTEVVNAGAWRLPFVLGGVLGLLAVWMRK
                     HLQETPIFMAIKQRRLLYQGLPLAEAWRSHKTLLLIGMGINWFLLASVAVVLLAMPKL
                     LQQQLGMSALQATTWQVCGIALQATGCIGFGYLADKRGISRSTWVGAGLMAVAATVFY
                     FGMQTLPVGVLAGAYGVMAVCAGSGASLGKMVVRFPAPIRLSGMAATYNVSSALLGGL
                     SLPLVAWLGQRFSWGAWLYLSLLCVMFALLGYWYAKRFEAACKSHS"
     gene            3212..>3706
                     /locus_tag="PK856_05145"
     CDS             3212..>3706
                     /locus_tag="PK856_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL01788.1"
                     /translation="MTEPTIQQYDDVHGNRWLNRDDYRTLSLSSLGGALEFYDFVIYV
                     FYAKIISELFFPSTLSPFWAMLNTYGIFAAGYFFRPLGGLVMAHFGDLVGRKRLFALS
                     IFLMALPTLMIGLMPTYASIGYAAPLLLLLMRIMQGIAIGGEIPAAWTFVSEHVPPKH
                     VGFAN"
     assembly_gap    3707..3716
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3717..4524
                     /locus_tag="PK856_05150"
     CDS             <3717..4524
                     /locus_tag="PK856_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL01789.1"
                     /translation="LTAGLSFGILLGALMSLAIAKLFDEVEIHDWAWRIPFVIGGLLG
                     FVTMYLRSYLKETPVFKAMQERKQLYQGLPVKEVLQNYRLSVVLGMLFTWFLTACVVV
                     VLLAMPQLLIGTFGYTRADAFVLQSTGIVMQVVGCVLFGWLADRMGGGKAMVLGSIAV
                     SVCALVYYNALGVWSANAIFITYACLGLAAGAVGVVPMLMVRMYAAPVRFTGISFSYN
                     VSYAIVGGLTLPVVQWLSQYSNIGAMYYVLALAVLCVVCSVIYMRKFEHA"
     gene            4814..>5159
                     /locus_tag="PK856_05155"
     CDS             4814..>5159
                     /locus_tag="PK856_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HRL01790.1"
                     /translation="MGSYSFLQVFTVVFALSIATTLFNKRALGLPAAIGVPIVSAVFV
                     FLLQWAVSLFGHNDYIHINIDEIEKAVRTFNFYDFLINGVICFILTSSALKFKITDLR
                     VYFKPIAILASIA"
BASE COUNT         1098 a         1097 c         1467 g         1487 t
ORIGIN      
        1 gcgagtttcg ctggatctgc tgatgcggaa tatgatgtgg tggtattggg cggtggccct
       61 ggcggctatt cggctgcgtt tgctgctgct gaccaaggct tgaaaacagc gattattgag
      121 cgctatgcga ctttgggtgg tgtgtgtttg aacgtgggct gtattccatc caaagctttg
      181 ttgcacaatg cagcggtgat tgatgaagtc aaacacttgg ctgccaatgg catcaagttt
      241 gccgaacctg aaatcgacat cgacatgttg cgtggttaca aggaaaaagt cataggcaaa
      301 ctcacaggtg gtttggcggg catggccaag gcgcgcaaag tggacgtaat ccgtggcaat
      361 gcgcaatttg tgtctgccaa ccattttgaa gtggccgaaa ccacaggcac aggctcagaa
      421 gagactggca gcaagaaaac cgtggcgttt aaaaatgcca tcattgccgc aggcagccgc
      481 gtgaccaaat tgccattcat acctgaagac ccgcgcattg tggattctac aggtgcgttg
      541 gagcttaaag gggtgccaga aaaaatgctg attatcggcg gtggcatcat cggcttggaa
      601 atgggcacgg tttacagcac attgggcgca cgcttggatg tggtggaaat gatggacggt
      661 ttgatgcaag gcgcagaccg cgacttggtc aaagtgtggc aaaaaatgaa tgagtaccgc
      721 tttgacaaca tcatgctgaa caccaaaacc gtgggcgtgg aagcgaaaga agacggcatt
      781 tacgtgacgt ttgaaggcgc gaaagcccca actgagccgc aacgctatga tttggttttg
      841 gtggcagcgg gtcgtcaacc caatggcttg ttaatcggcg cagacaaagc aggcgtggcc
      901 gttaccgacc gcggcttcat caatgtcgac aaacaacagc gcaccaatgt gccgcacatt
      961 tatgcgatag gtgacattgt cggtcaacca atgttggcgc acaaagccgt gcacgaaggc
     1021 catgtggccg ctgaaaactg tgccggtcac aaagccttct ttgatgcgcg cgtgattcct
     1081 ggtgtggcgt ataccgaccc tgaagtggcg tgggtgggcg tgaccgaaga aatcgccaag
     1141 cgtgatggca tcaaaatcac caaatcggtg ttcccttggg ccgcttcagg ccgtgccatt
     1201 gccaatggcc gcgatgaggg catgaccaaa ttgattttcg atgccgaaac tggcttgatt
     1261 atcggcggtg gcattgtggg cacgcacgca ggcgacatga taggcgaagt gtgcttggcg
     1321 attgaaatgg gttgtgatgc cgaagacatc ggcaaaacca tccatcctca tccgactttg
     1381 ggtgagtcca tcggcatggc agctgaagtg gctttgggtg tttgtaccga tttgccgccg
     1441 atgcgcaaaa aataaaacgc attatccaat ggctgtctgc tttcgcaggc agccattttt
     1501 ttatccatta cacacaacac atggaaacac acatgcaaca ccacaatgta aacgctgatg
     1561 ttttacccgc acggcgaggc ttggcaaggc aagacagcaa ggttttggcc ttggcatctt
     1621 tgggcggcgc tttggagttt tacgatttca tgatttttgc gctgatggcg ccgatactca
     1681 gccaattgtt ttttcccacc agtttgggtg atggttgggc tttgttttat acctatgcca
     1741 tttttgcgac aggttacttc atgcggccag tgggtggctt ggtgatggca catctgggtg
     1801 acaggttggg acgcaaacgc atgtttacct ggtctttgtt gttgatggcg ctgccgactt
     1861 tggctatggg tttgttgccc acttatgccc aaatcggttt ggttgcgccg ttgctgttat
     1921 tgctttgccg catggttcaa gcagtggcca tcggtgggga agtacctgct gcatggactt
     1981 atgtatccga acaagtccct gttaaccaag tgggtttggc caatggcttg ctcacagcag
     2041 ggctgtcagt ggggattttg ttgggctctt tgtcagtttt ggcattacag gccgcattca
     2101 gtacagaagt ggtgaatgct ggggcatggc gtttgccatt tgtattgggt ggggtgttgg
     2161 gtttgctggc ggtgtggatg cgaaaacatt tgcaagaaac gccgatattc atggccatca
     2221 aacagcgccg actgctgtac caaggtttgc ctttggctga ggcttggcgc tcgcacaaaa
     2281 cgctgttgct cattggcatg ggcatcaatt ggtttttatt ggcaagtgtg gcggtggttt
     2341 tgttggccat gcccaagctg ctgcagcaac aactgggcat gagtgcgctg caagccacca
     2401 catggcaagt gtgtggtatc gctttgcaag cgacagggtg cataggcttc ggctacttgg
     2461 ccgataaacg cggtatcagc cgcagtactt gggtaggagc tggcctgatg gcggtggcag
     2521 cgacggtttt ttactttggc atgcaaacat tgcctgtcgg agtgttggca ggagcatacg
     2581 gtgtgatggc tgtgtgtgcg ggtagtgggg cgagcttggg caaaatggtg gtgcgttttc
     2641 ccgcacccat tcgtttgagt ggcatggctg cgacttacaa tgtttcatcg gcgttgctgg
     2701 gaggcttgtc tttgccattg gtggcatggc tggggcagcg gtttagctgg ggcgcttggc
     2761 tgtatctcag tttgttgtgt gtaatgtttg cattgttggg ctattggtat gccaagcgct
     2821 ttgaagcggc ctgtaaatcc cattcttaaa gattcataca cagaatatgt gtataaggat
     2881 gttgataaca attgatatgg ctttggtttc agtggcttag gtttgtgctc attttatggt
     2941 caatgcaaac cgcgcataga aatcatgaaa tgagcatttt attaggatgg tcatgatggc
     3001 ttatattttg taatgctttt gactagcttg ctcagtctgg gcatggattg acatattttg
     3061 gatatgtatt aatatattat ttcaaattca atagttaaca ttatttgttt gaaataatat
     3121 tgctgaatgc tgtgcttgaa taatacaaaa gctgaaatat gttgaaatca gcgcgctaat
     3181 cagtaagtgt ccacaatgta tgaagcagaa tatgacagaa cccactatcc agcaatacga
     3241 cgatgtgcac ggtaaccgtt ggctcaatcg cgatgattac cgtactttat cgctttcttc
     3301 tcttggcggc gcccttgaat tttacgattt tgtgatttat gtgttttacg ccaaaatcat
     3361 ttcggaattg ttcttcccca gtactttgag cccattttgg gccatgctca atacctatgg
     3421 tatttttgcc gcgggctatt tcttcaggcc gctgggtggt ttggtgatgg cgcattttgg
     3481 tgatttggtt ggccgcaaac gcctgttcgc cttgtccatc tttctgatgg ccttgccgac
     3541 cttgatgata ggcttgatgc cgacttacgc aagcattggt tatgccgcgc cgctgctctt
     3601 gttattgatg cgcatcatgc aaggcattgc catcggtggg gagattcctg cagcgtggac
     3661 gtttgtgtct gagcatgtgc cacccaaaca cgttggtttt gccaatnnnn nnnnnngttg
     3721 accgcaggtt tatcgtttgg cattttattg ggggccttga tgtctttggc catcgccaag
     3781 ctgtttgatg aggttgagat tcatgattgg gcttggcgaa ttccctttgt catcggtggc
     3841 ttattgggtt ttgtcaccat gtatttgcgc tcttatctga aagaaacgcc tgtcttcaag
     3901 gccatgcaag agcgcaaaca gctctatcaa ggtttacctg tgaaagaggt gttacaaaac
     3961 taccgtttgt cggtagtgtt gggcatgttg tttacttggt ttttaaccgc gtgtgtggtg
     4021 gtggtgttgc tggccatgcc gcaattgctc attggcacgt ttggctacac ccgcgccgat
     4081 gcgtttgtgt tgcaatccac cggcatcgtg atgcaagtgg tgggttgcgt gctctttggt
     4141 tggttggccg accgtatggg cggcggcaaa gccatggttt tgggcagcat cgcggtgtct
     4201 gtgtgcgcct tggtgtatta caacgccttg ggcgtgtgga gcgccaatgc catcttcatc
     4261 acctatgcgt gcttgggttt ggctgcgggt gctgtgggcg tggtgccgat gttgatggtg
     4321 cgcatgtatg ccgcgcctgt gcgctttacg ggcatttctt tttcttacaa tgtgtcgtac
     4381 gcgatagtcg gcggtttgac tctgccggtg gtgcaatggc tgagccaata cagcaacatc
     4441 ggtgcgatgt attacgtgct ggccttggcg gtgttgtgtg tggtgtgcag cgtgatatac
     4501 atgcgcaagt ttgaacacgc ttgatgtaca caagctttaa aatgcggcct gtaaggttta
     4561 caggccgctt ttttgtggtg tggcagatgc tttgtgacca gcagtttgtt gccagtaagc
     4621 ctgtatgaag tgttattctt gatgaatggc ggtaattttc agcataaaac agccaatttc
     4681 acaaaaacca acatggtttc tgcttagatg ctaatgattc ttgggctcga tttcgagtaa
     4741 aatgtttacg ctgcttatcg agtcttgctt gataacatcg tcacgaaacg aaatacaaaa
     4801 gaggccaaca cacatgggaa gctattcctt cttacaagtt tttacggtgg tattcgcctt
     4861 gtcgattgcc accacattgt ttaacaagcg ggcattgggt ttgcctgcgg ccatcggtgt
     4921 gcccatcgtg tcggcggtgt ttgtgttttt gctgcaatgg gcggtgagcc tgtttgggca
     4981 caatgattac atccacatca acattgatga aattgaaaaa gccgtgcgca ccttcaattt
     5041 ctacgatttc ttaatcaatg gggtgatttg ctttattttg acctcatccg cgctcaaatt
     5101 caaaatcacc gatttgcgcg tgtatttcaa accgattgcg attttggcca gcattgcct
//
LOCUS       DAOMVK010000113         5146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_4497_length_5146_cov_3.180711, whole genome shotgun sequence.
ACCESSION   DAOMVK010000113 DAOMVK010000000
VERSION     DAOMVK010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4497_length_5146_cov_3.180711"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="PK856_05160"
     CDS             <1..616
                     /locus_tag="PK856_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HRL01791.1"
                     /translation="LDPYRPLDADKEHIQIQAIAAPYQWVFVYPNENIATVNEIYFPA
                     NQPVNFTITSDFSMNSFFIPQLGGQVYAMAGMKTQLHLIANEPGQFYGMSANYSGHGF
                     SKMKFQAHAVSEAEYQNWIKQAKASNNTLTMQKFKSLQDPVHQTAVGHHAKRTEAPKP
                     NPVQYFGSVENNLLQNIVDQYMIAKHEKATGASAPVAASAAGGN"
     gene            620..2617
                     /gene="cyoB"
                     /locus_tag="PK856_05165"
     CDS             620..2617
                     /gene="cyoB"
                     /locus_tag="PK856_05165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HRL01792.1"
                     /translation="MSFLGRLSLDAIPADPIVQLTMVAMAVGGIGLLAAITYFKKWGY
                     LWSEWFTSVDHKRLGIMYVIVSLVMLVRGFADAFMMRLQLSLAANGGPGYLPPEHYDQ
                     IFTAHGVIMIFFVAMGLVVGFMNVLVPLQIGARDVAYPLLNNFSFWLFVSGVVLVMMS
                     LVLGEFAATGWLAYPPLSNIEMSPGVGVDYYIWALQLSGLGTLLTGVNMVVTILRMRT
                     PGMSLMQMPIFTWTSLVAAMMILATFPVLTSTLAMLTVDRLLGFHYFTNELGGSPMLY
                     VNLIWTWGHPEVYILVLPAFGIFSEVVATFSGKRLFGYKGMVYASGAIAVLSMVVWVH
                     HFFTMGAGSSVNAFFGIMTMIIAIPTGVKIFNWIFTMYKGKIRFEVPMLWAVGFLVTF
                     TVGGMTGVLLSIPPADFVVHNSLFLIAHFHNTIIGGVVFGLMAGLHFYWPKMFGFKLG
                     DKLGRASFWCWFWGFWLAFVPLYILGFMGMTRRMNTIGLDHPEWTPYLHVAFFGAVVI
                     AAGIGLTVLNILLSIKNHKKNRDVTGDPWQGRTLEWSLSSPPPFYNFAKLPEINDIDQ
                     FWYDKEHGKAYQTDGKYEEIHMPKNRTIGVVMTVLLTASAFGVIWHMWWLAALGLAGT
                     IVSLIVSSFNTKHDYYVDVETISKTENAHLTAVREYQESRG"
     gene            2629..3249
                     /gene="cyoC"
                     /locus_tag="PK856_05170"
     CDS             2629..3249
                     /gene="cyoC"
                     /locus_tag="PK856_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HRL01793.1"
                     /translation="MNVNDAHHHDDAHHDHDAVVASNASLGFWIYIMTDLILFATLFI
                     GFQVLREHSAGGPTEYEVFKESLGFVFVETLVLLTSSFTFGMAHLAQAQKNMAKLQFW
                     LFATFVLGAVFIGMEVYEFSHLIHIGEGPQTSAFLSAYFGLVGTHGLHVSSGLIWLAV
                     IMFQIKKFGLTPTNNRRLMMLSIFWHFLDIVWICVYTEVYLLGAVQ"
     gene            3246..3584
                     /gene="cyoD"
                     /locus_tag="PK856_05175"
     CDS             3246..3584
                     /gene="cyoD"
                     /locus_tag="PK856_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HRL01794.1"
                     /translation="MSGNNHAVANQHQHDDHGSTKQYVIGFIISIVLTVVPFFLVMNG
                     GFPKALTVGVLALAMLAQVWVQLVWFLHMKRTDEQRWHITSFWYTFMTIAILFIGSVW
                     ILYHLHNHMM"
     gene            3592..4482
                     /gene="cyoE"
                     /locus_tag="PK856_05180"
     CDS             3592..4482
                     /gene="cyoE"
                     /locus_tag="PK856_05180"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003348.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HRL01795.1"
                     /translation="MQTTTFKRYWGLIKPGIVFGNMIPVLGGFLLAEPSWKDWPTLLW
                     VMLATFCLVGSACVFNNYYERDIDGLMTRTQNRVLVHDGIPKVQVFGIALVLLVSALV
                     ILAHINWVTFALGIFGFVVYVLIYTLLLKRHSIHQTTLGSFSGACPPVMGYCSIAGVF
                     NIDAFLIFMLFAFWQIPHSYAIAVFRQQDYANAQIPLLPLVKGVGHTKKAMIVYWALY
                     LATGVALYAYGTVGWVFLAIFGYYGLRWGAIGWQGFDAEDNNKWARRFFFYSIKVMMM
                     FSLGIVVDTLVKYLIPSIYF"
     gene            4879..>5146
                     /locus_tag="PK856_05185"
     CDS             4879..>5146
                     /locus_tag="PK856_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HRL01796.1"
                     /translation="MKTAEIRQKFLSFFESKGHAIVSSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYTRAVTAQKCVRAGGKHNDLENVGYTARHHTFF"
BASE COUNT         1160 a         1123 c         1274 g         1589 t
ORIGIN      
        1 attggatcca taccgtccct tggatgccga caaagagcac attcaaattc aagcgattgc
       61 cgcaccgtac caatgggtat tcgtgtatcc aaacgaaaac attgccacgg tcaatgagat
      121 ttatttccca gccaatcagc ctgtgaattt caccatcact tctgacttct cgatgaactc
      181 gttcttcatc ccgcagttgg gtggtcaggt ttacgcgatg gcaggcatga aaacgcaatt
      241 gcatttgatt gcgaatgagc caggtcaatt ctatggcatg tcagccaact acagtggtca
      301 tggtttctca aaaatgaaat tccaagccca tgctgtgtcg gaagctgaat accaaaattg
      361 gattaaacag gccaaagcat caaacaacac tttgaccatg caaaaattca agtctttgca
      421 agatcctgtg catcaaactg ctgttggtca ccatgccaaa cgcactgagg cacctaagcc
      481 caatcccgtt cagtatttcg gcagtgttga aaacaacttg ctgcaaaaca ttgtggatca
      541 atacatgatt gcaaaacatg aaaaagccac tggcgccagt gccccagttg cggcttcagc
      601 tgcaggagga aattaagcta tgtcttttct aggcagactt tctttggatg cgattccagc
      661 cgacccgatt gtccagttga caatggtggc catggctgtg ggtggtatcg gattactggc
      721 ggccatcact tattttaaaa agtggggcta tttgtggagt gagtggttta cttctgtgga
      781 ccacaaacgc ttgggcatca tgtacgtcat cgtctctttg gtgatgttgg tgcgtggttt
      841 tgctgacgcg ttcatgatgc gtttgcaact gtctttggct gccaatggcg ggccaggcta
      901 tttgcctcct gaacactatg accaaatctt taccgctcac ggcgtgatca tgattttctt
      961 cgtggccatg ggtttggtag tgggtttcat gaacgtgttg gtgccattgc aaattggtgc
     1021 ccgcgacgtg gcttaccctt tgctgaataa cttcagcttc tggttgttcg tgtcaggcgt
     1081 ggttttggtg atgatgtctt tggtattggg tgagtttgca gccacaggtt ggttggctta
     1141 tccgccattg tctaacattg aaatgagtcc tggtgtgggt gtggattact atatctgggc
     1201 cttgcagttg tcaggcttgg gtactttgtt gacgggtgtc aacatggtgg taaccatctt
     1261 gcgcatgcgc acgcctggca tgtctttgat gcaaatgcct atcttcactt ggaccagctt
     1321 ggttgctgcc atgatgatct tggcgacctt cccagtgttg acttctacct tggccatgtt
     1381 gacggtagac cgtttgttgg gcttccatta cttcaccaac gaattgggtg gtagcccaat
     1441 gttgtatgtg aacttgattt ggacttgggg tcaccctgag gtttacatct tggtgttgcc
     1501 tgcttttggt attttctcag aagttgtggc cactttctca ggcaaacgtc tgtttggtta
     1561 caaaggcatg gtttatgcca gtggcgccat tgcagtattg tctatggtgg tttgggtgca
     1621 tcacttcttc accatgggtg cgggttctag tgtcaatgcc ttctttggca tcatgaccat
     1681 gattattgcc atcccaacag gtgtgaaaat ctttaactgg attttcacca tgtacaaagg
     1741 caagattcgc tttgaagtgc ctatgttgtg ggctgtgggt ttcttggtaa cctttaccgt
     1801 tggtggtatg acgggcgtat tgttgtctat tccacctgct gactttgtgg tacacaactc
     1861 tttgttcttg attgcccact tccataacac cattatcggt ggtgtggtat tcggtttgat
     1921 ggctggtttg catttctact ggccaaaaat gttcggtttc aaattgggtg acaaattggg
     1981 ccgtgcatca ttctggtgct ggttctgggg cttctggttg gctttcgtac cattgtacat
     2041 cttaggtttc atgggcatga ctcgccgtat gaacaccatc ggtttggacc atcctgagtg
     2101 gacaccgtac ttgcacgtgg catttttcgg tgcggtagtg attgctgctg gcattggttt
     2161 gactgtgttg aacatcttgt tgtctatcaa aaaccacaag aaaaaccgcg acgtgaccgg
     2221 tgacccttgg caaggccgta cgttggagtg gtctttatct tctcctcctc cgttctacaa
     2281 ctttgccaaa ttgccagaaa tcaatgacat tgaccaattc tggtatgaca aagaacatgg
     2341 taaagcttac caaacagatg gcaagtatga agaaattcac atgcctaaaa accgtaccat
     2401 tggtgtggtg atgacagtgt tgttgacagc cagtgcattt ggtgtgattt ggcacatgtg
     2461 gtggttggct gcattgggtt tggctggcac catcgtgtct ttgatcgtgt cttcattcaa
     2521 taccaagcat gactattatg tggatgtaga gaccattagc aaaaccgaga atgcgcattt
     2581 gaccgccgtt cgtgaatacc aagaatctcg aggttaatta tgacaacaat gaatgtaaat
     2641 gatgcacatc atcacgatga tgcccatcac gatcatgatg ctgtagtggc ttccaatgcc
     2701 tctttgggct tttggatcta catcatgacc gacttgattt tgttcgccac cttgttcatt
     2761 ggttttcaag tgttgcgcga gcattcagct ggcggcccaa ccgaatacga agtcttcaaa
     2821 gaaagtttgg gctttgtatt cgtggaaacc ttggtgttgt tgaccagtag tttcactttc
     2881 ggcatggcgc atttggccca agcccaaaaa aacatggcca aattgcaatt ttggttgttt
     2941 gccacgtttg tattgggcgc cgtcttcatc ggtatggaag tgtatgagtt cagccatctg
     3001 attcacattg gcgaaggtcc acaaaccagc gcattcttga gtgcttactt tggtttggtg
     3061 ggtacccacg gcttgcacgt gagctcaggc ttgatctggt tggctgtcat catgttccaa
     3121 atcaaaaaat ttggtttgac acctaccaac aaccgccgcc tgatgatgtt gagtattttc
     3181 tggcacttct tggacatcgt gtggatctgt gtctacactg aagtttactt attgggagca
     3241 gttcaatgag tggaaacaat catgctgtcg ccaatcagca ccaacatgac gaccatggct
     3301 ctaccaaaca atatgtgatt ggtttcatca tctctattgt gttgactgtg gtgccattct
     3361 tcttggtgat gaatggtggc tttccaaaag ccttgaccgt gggtgtattg gctttggcca
     3421 tgttggctca ggtttgggta cagttggtgt ggttcttaca catgaaacgc actgatgagc
     3481 aaagatggca catcacttcg ttctggtaca cttttatgac cattgcgatt ttgttcatcg
     3541 gttcggtgtg gattctgtac catttgcaca accacatgat gtaatcagat tgtgcaaaca
     3601 acgacgttta agcgctattg gggcttaatc aaaccgggaa ttgtgtttgg taacatgatt
     3661 cccgtgttag gtggcttctt actggctgaa ccaagttgga aagactggcc aacattgctt
     3721 tgggtgatgt tggccacctt ctgtttggtc ggttcggctt gtgtgttcaa taattattat
     3781 gaacgcgaca ttgatggctt gatgacgcgt acccaaaacc gtgtgttggt acacgatggc
     3841 atacccaaag tacaggtgtt tggcatcgct ttggtattgc tggtcagtgc cttggtgatt
     3901 ttggcccaca tcaattgggt gactttcgcc ttgggcattt ttggctttgt ggtgtatgtg
     3961 ttgatttaca ccttattgct caagcgccat tccattcatc aaaccacctt gggcagtttt
     4021 tcaggtgcgt gcccacccgt gatgggctat tgcagcattg caggtgtgtt caatattgat
     4081 gcgttcttga tttttatgtt gttcgccttt tggcaaatcc ctcattctta tgccattgct
     4141 gtgtttcgcc agcaagacta tgcgaatgca caaattccct tattgccttt ggtaaaaggc
     4201 gtgggccaca ccaaaaaagc catgatagtg tattgggctt tgtacttggc aactggcgtg
     4261 gctttgtatg cttatggcac cgtgggctgg gtatttttgg ccatatttgg ctattacggc
     4321 ttgcgttggg gagccatagg ttggcaagga tttgatgccg aagacaacaa caaatgggcg
     4381 cgacgctttt tcttttattc gataaaagtg atgatgatgt tcagcctggg cattgtggtt
     4441 gacaccttgg tgaaatattt gattcccagt atttattttt gatgggtact accgttacag
     4501 gccgcttgat gcggcctgta atttttttgg ttaagaaaaa ccatcattgg cttggtattg
     4561 aattcacaca tttccatggc cattgtttgg tattgcgcag tcagcaatgt ctgcctcagc
     4621 caacaataca gaacaggtgt gttgagacag ggtttcttct ttgaagccac gccaccaatg
     4681 gcatggcggt gcttacaggc cgcatgttgg gctgatgaac aatgcaacac cttgattgtg
     4741 gcgacagtgt cgttcatgca atgcacaaat tttaaccatc agtgcttacc caacccccca
     4801 gatatgatag tatttagctc gattgccagt acgctggcca cagatttctt ttaacttcat
     4861 aaacattaag acaccagcat gaaaacagcc gaaatacgcc aaaaattcct ctcgtttttc
     4921 gagagtaagg gacacgctat cgtgtcttca agctcgctcg tgccacatga cgacccgacc
     4981 ttgttattca ccaatgctgg catgaaccag ttcaaagatg tgtttttggg ctttgataaa
     5041 cgcccataca cccgcgccgt gaccgcgcaa aaatgcgtgc gcgcaggggg taaacacaac
     5101 gatttggaaa acgtcggcta cactgcacgc caccatacct tctttg
//
LOCUS       DAOMVK010000114         5143 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_4503_length_5143_cov_3.155071, whole genome shotgun sequence.
ACCESSION   DAOMVK010000114 DAOMVK010000000
VERSION     DAOMVK010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4503_length_5143_cov_3.155071"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="PK856_05190"
     CDS             <1..620
                     /locus_tag="PK856_05190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRL01797.1"
                     /translation="QQAELSKLNKNQADANVRAPISGVVYDKLVNAGELVNAGGQILK
                     IAQTNVLEIAATVSSEQVTFIKEGQEVTFTVATGAPVQTGRIVRINPVADANTRQFTV
                     FIQVQNADGAVKAGQFAQGNIVLNQVAQAMVVPETAIKQAEGQAFVYVVSQGKLTKRP
                     VKVVLQDPSTARAAVEGLKAGEAVLRTELLGIKVGDAVSLPPAGK"
     gene            622..3753
                     /locus_tag="PK856_05195"
     CDS             622..3753
                     /locus_tag="PK856_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRL01798.1"
                     /translation="MWLTRLSINNPYFAAVLVLATVVLGFFSIFKLPVEAFPDVRFPV
                     AMVSTAYEGASPEVVESDVSKPIEEALNTINGIKNIRSYSFEGSSVVIAEFELDVDTA
                     KSVQDVRDKVGAVGGSFDRDIDTPVVSEFNPMDESMLNIAMGTDKVSLRELTDWTDNT
                     LKKRLQTVMGVGEVEIVGGTERQITVAVKPEQLRSLNVNIADVNNAIAAANSDYPSGT
                     VRQQGNEIAIRVTGKIKHPESFANIVVAHRDGSLIRVRDVADVHDGIAEATSIATLNG
                     KPAVSLNIRATRGANVVEVADGVKAMLDEEIKRGPPDLKVVYTYDQSDDIRNSVNEVR
                     DTLIEGVFLTVLIVFLFLGTWRSTVITGLTLPVSLIGAMWAISLMGFTINLMTLMALS
                     LAIGLLIDDAIVVRENIVRHVQLGKSHFQAALDGTQEIGLAVLATTLCIVAVFLPIGY
                     MDGIIGKFFHQFGLTVAAAMVISTVVSLSLDPMLSSIWHDPKHGKRGPIGRAVDRFDR
                     WIDEVAERYTGWVRWCLHHPIKVLAVVTVMMLGSFALVGTGMVGGEFMPQQDRGKIKV
                     AFKTATGSTLEYTTQKTTEVRDAIGHFPEVKSVAADVGAAGFGNGKTNSSLSIDVGDK
                     NERERDVHQLMKDMRVELDKIAGIEITSIEAMGQAGPGGKIVNVGLRGSNSEELTVAA
                     KQVEAAMAKIPGVTDIENSESDADPSLDVLIDRDAASALGINLATLGDTLKTLYAGSK
                     SSSWEAPDGNSYDVQLRIPQDARTAELLKQTTIPATGSDGQVHMVSLDSIATIKPGIS
                     PRQINRINLQREISLTANIAAGYNNSEVFSQIDAFKSELQLPNSVTLVQEGASKDMQE
                     SGLYALQALALGIIFIYLILVAQFRSFTLPITIMMSLPLIFIGVFVALWLTGNTLNMF
                     SIIGIVMLMGLSAKNGILLVDFINHARLNVGMDRLEAIIEAGKVRLRPIVMTSLAMIF
                     GMLPLALGNGEGSETRQAMAYAIIGGLITSTMLTLIVVPIVYVYLDNIRGWIHKTLKR
                     INHHDGGENPSAS"
     gene            3830..>4735
                     /locus_tag="PK856_05200"
     CDS             3830..>4735
                     /locus_tag="PK856_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01799.1"
                     /translation="MRLNQKCWLVLWCVWLGLQAAYANMASPTRFDEPARSTLEVVPS
                     QQLQVLHESIVAQVTPDLRRARFVVQYQINNPHGKALLPLRFYHYQGGQGAADFEVWV
                     DEKKVPLTPTSNSALDATSDITAASDVLPAATVDMALQTQDFSVVLPQGVHVVKVAYW
                     AYAGMHGGGDFKQAEFAYDLRPSLQWQSFGMVDIQVLLPQADLALNAEWAPTTKTPTL
                     WAWQVQPSAEISEFVFRLDYPVMWWQRVLVAIAPWLWVVCLLLPLPFLCRHLRRLQRA
                     GRKRAWVVTLWTAVVALPLLVLAMYA"
     assembly_gap    4736..4745
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4746..4938
                     /locus_tag="PK856_05205"
     CDS             <4746..4938
                     /locus_tag="PK856_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01800.1"
                     /translation="VLVPLLLQWGLGAVLSPSYGDAWVYMGMALLVLGYPVAILVYTL
                     VLLAFGRIAPPRATQPPVV"
BASE COUNT         1172 a         1234 c         1490 g         1237 t
ORIGIN      
        1 cgcaacaagc agaattgtcc aagctgaaca aaaaccaagc cgatgccaat gtgcgtgcgc
       61 ccatcagtgg cgtggtgtac gacaaattgg tcaatgcggg cgaattggtc aatgcaggtg
      121 ggcagatttt gaaaattgcc caaaccaatg tgttggaaat cgccgcgacg gtgtcgtctg
      181 aacaagtgac gtttatcaaa gaaggacaag aggtaacgtt caccgtggcc acgggtgcgc
      241 ctgtgcaaac agggcgcatt gtgcgcatca atcctgttgc cgatgccaat acgcgccaat
      301 tcaccgtgtt catacaggtg caaaacgccg atggggcggt gaaagcgggg cagtttgccc
      361 aaggcaatat tgtgctcaac caagtggcgc aagcgatggt ggtgcctgaa acggcgatta
      421 aacaagccga aggacaagcg tttgtgtatg tggtgagcca aggcaagctt actaagcggc
      481 ctgtaaaagt tgtgttgcaa gacccaagca ctgcccgcgc ggcagtagaa ggcttgaaag
      541 cgggtgaagc ggtattgcgc accgaattgt tgggcatcaa agtgggcgat gccgtgagtt
      601 tgccgccagc ggggaaatga tatgtggcta acccgcctga gcattaacaa tccttatttc
      661 gccgcggtgt tggttttggc cacggtggtg ttgggttttt tttcgatatt caaattgcct
      721 gttgaggcgt ttcctgatgt gcgttttcct gtggcgatgg tatccacagc ttacgaaggc
      781 gcttcgcccg aagtggtgga aagcgatgtg tccaagccga tagaagaagc gctgaacacc
      841 atcaacggca tcaaaaacat ccgctcatat tcgtttgaag gctcatcggt ggtgattgcc
      901 gaatttgaat tggatgtgga cacggccaaa tcagtgcaag acgtgcgcga caaagtgggt
      961 gccgtcggtg gctcattcga ccgcgacatc gacacgccgg tggtgtcgga attcaatcct
     1021 atggacgaat ccatgctcaa cattgccatg ggtacggaca aagtcagcct gcgcgaattg
     1081 acggattgga ccgacaatac cttgaaaaaa cgcctgcaaa cggtgatggg cgtgggcgag
     1141 gtggaaatcg tcggtggcac tgagcgccaa atcacggtgg cggtgaagcc tgagcaattg
     1201 cgcagcctga atgtgaacat tgccgatgtg aacaatgcga ttgccgcagc caacagcgat
     1261 tacccatcgg gcacggtgcg ccaacaaggc aatgaaatcg ccatccgcgt tacaggcaaa
     1321 atcaaacatc ctgaaagctt tgccaacatc gtggtggccc atcgcgatgg cagcctcatt
     1381 cgtgtgcgcg atgtggccga tgtccacgat ggcattgccg aagccaccag cattgccact
     1441 ttgaatggta agcccgcggt gtcactgaac atccgtgcca cgcgtggcgc gaatgtggtg
     1501 gaagtggccg atggtgtgaa agccatgttg gatgaagaaa tcaaacgtgg cccgcccgat
     1561 ttgaaggtgg tgtataccta cgaccaatcc gacgacatcc gcaactcggt gaatgaagtg
     1621 cgcgacacct tgattgaagg cgtgtttctg accgtgttaa tcgtgttttt gttcttaggc
     1681 acatggcgct ccaccgtgat tacaggtcta accttgcctg tgtctttaat cggcgcgatg
     1741 tgggcgattt ctttgatggg cttcaccatc aatttgatga cgctgatggc gctgtctttg
     1801 gccatcggtc tattaataga cgatgcgatt gtggtgcgcg aaaacattgt gcgccacgtg
     1861 caattgggca aaagccattt tcaggccgct ttagacggca cgcaggaaat tggcttggca
     1921 gtattggcca caactttgtg tatcgtggcg gtgtttttgc ccataggcta tatggacggc
     1981 atcattggta agtttttcca ccaatttggt ttgactgtgg ccgccgcgat ggtgatttca
     2041 accgtggtgt ctttgagctt agacccaatg ctgtcgagca tttggcacga ccccaaacac
     2101 ggcaagcgcg ggccgattgg ccgtgcggta gaccgttttg accgctggat agacgaggtg
     2161 gctgagcgct atacgggttg ggtgcgttgg tgtttgcacc acccgattaa agtcttggcg
     2221 gtggtgacgg tgatgatgtt gggcagtttc gccttggtgg gcacgggcat ggtgggcggc
     2281 gaattcatgc cgcaacaaga ccgtggcaaa atcaaagtgg cgttcaaaac cgcaacaggt
     2341 tcgacgctgg aatacactac ccaaaaaacc acagaagtgc gcgatgccat cggccatttc
     2401 cctgaagtga aaagcgtggc ggcagatgtg ggcgcggcag gttttggcaa tggcaaaacc
     2461 aacagctcgc tcagcataga cgtgggcgat aaaaacgagc gtgagcgcga tgtgcaccaa
     2521 ttgatgaaag acatgcgggt agagctagac aaaattgctg gtattgaaat cacctcgata
     2581 gaagccatgg gtcaagcagg gccaggcggt aaaatcgtca acgtgggttt gcgcggcagc
     2641 aacagcgaag aattgacggt agcggcgaaa caagttgaag ctgcgatggc gaaaatccct
     2701 ggcgttaccg acattgaaaa cagcgaatcc gatgccgacc cttctttgga tgtgttaatc
     2761 gaccgcgatg ccgcatcggc tttgggcatc aatttggcga cattgggcga caccttaaaa
     2821 accctgtatg cgggcagtaa atcctctagc tgggaagcgc ccgatggcaa cagctacgat
     2881 gtgcaattgc gcattccaca agacgcgcgc accgccgaat tgctcaagca aaccaccatt
     2941 cctgccacag gcagcgatgg ccaagtgcac atggtgagct tggacagcat cgccaccatc
     3001 aagcctggca tcagcccgcg ccaaatcaac cgcatcaatt tgcagcgcga aatcagctta
     3061 accgccaata ttgccgcggg ttacaacaac agcgaggtgt tcagccaaat cgatgcgttt
     3121 aaatccgaat tgcaattgcc aaacagcgtg accttggtgc aagagggggc gagcaaagac
     3181 atgcaagaat ccggcctgta cgccttacaa gccttggcct tgggcatcat cttcatttac
     3241 ctgattttgg tggcacagtt tcgcagcttc accttaccca tcaccatcat gatgtcgctg
     3301 cccttgattt tcattggcgt gtttgtggcg ctgtggctca cgggcaacac gctgaatatg
     3361 ttctccatca tcggcatcgt gatgttgatg ggcttgtcgg cgaaaaacgg tattttgttg
     3421 gtcgacttca tcaaccacgc gcgcctgaat gtgggcatgg acagattgga agccatcatc
     3481 gaagcgggca aagtgcgttt gcgcccaata gtgatgacca gcttggcgat gatatttggc
     3541 atgttgcctt tggccttggg taatggcgaa ggctcggaaa cgcgccaagc catggcctat
     3601 gccatcatcg gcggcttgat tacttcaacc atgttgactt taatcgtggt gccgattgtg
     3661 tatgtgtatt tggacaatat ccgcggctgg atacacaaaa ccctcaaacg catcaatcac
     3721 cacgatggcg gcgaaaaccc atctgcatct taatttaagc ggcctgtaaa tcgtttacag
     3781 gccgcttttt atatgggcct gttgtcggtg gtgtgattaa ggagtgtgta tgcgactgaa
     3841 tcaaaagtgc tggttggtat tgtggtgtgt gtggctgggt ttacaggccg cttatgccaa
     3901 tatggcctcg cccacacggt ttgatgagcc tgcacggtcg accttggagg tggtgccaag
     3961 tcagcaattg caggtattgc atgaaagcat tgtggcacaa gtgacaccag acttgcggcg
     4021 ggcacgtttt gtggtgcaat accaaattaa caatccacat ggcaaggcat tgttgccact
     4081 gcgtttttac cattatcaag gtgggcaagg tgcggcggat tttgaggtgt gggtggatga
     4141 gaaaaaagtg ccgcttacgc ccacatcaaa ttctgctttg gatgccactt ctgatatcac
     4201 agcggcgagc gatgtgctgc ctgctgctac agtagacatg gccttgcaaa cacaagactt
     4261 cagtgtggtg ttgccgcagg gtgtgcacgt ggtgaaagtg gcatactggg cttatgcggg
     4321 catgcatggt ggtggcgatt tcaaacaggc agagtttgcc tacgatttgc gcccgagttt
     4381 gcaatggcaa tcatttggca tggtagacat acaggtgttg ttgccacagg ctgatttggc
     4441 tttaaacgcc gaatgggcgc ccaccaccaa aacgccaaca ctgtgggcgt ggcaagtgca
     4501 gcccagtgcc gaaatctctg aatttgtgtt tcgcctagat tatcccgtga tgtggtggca
     4561 acgggtgttg gtggccattg caccgtggct gtgggtggtg tgtttgctgt tgcctttgcc
     4621 gtttttgtgt cgccatttac ggcgtttgca gcgagcaggg cgtaagcgcg cttgggttgt
     4681 caccttgtgg acagcggtgg tggcgttgcc tttgcttgtg ctcgccatgt atgcgnnnnn
     4741 nnnnnggtgc tggtgccgct gctgctgcaa tggggcttgg gcgctgtgtt atcgcccagt
     4801 tatggcgatg cttgggtgta tatgggcatg gccttgctgg tattgggata ccctgtcgcc
     4861 attttggtgt ataccttggt gttgctggct tttgggcgca ttgccccgcc gcgtgccaca
     4921 cagccgcctg tggtatgaaa tgcacacaag cggcctgtaa acacggttag ggttttacag
     4981 gccgcttcta gagcctctat actccgcacc cattacttca acccttaact ggattttcat
     5041 cctatgccac gtttacacat tcaaccgaat taaacgacgc attctttatt ggatgcgtgt
     5101 gtgtctgcac catcctctca tccgcagtgc ccactttatc gct
//
LOCUS       DAOMVK010000115         5134 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_4514_length_5134_cov_3.322898, whole genome shotgun sequence.
ACCESSION   DAOMVK010000115 DAOMVK010000000
VERSION     DAOMVK010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 5134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5134
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4514_length_5134_cov_3.322898"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1363
                     /gene="lon"
                     /locus_tag="PK856_05210"
     CDS             <1..1363
                     /gene="lon"
                     /locus_tag="PK856_05210"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRL01801.1"
                     /translation="AKATGRQYVRMALGGVRDESEIRGHRRTYIGSMPGKILQNMAKV
                     GVKNPLFLLDEIDKMGQDFRGDPASALLEVLDPEQNHSFVDHYAEVEYDLSDVMFIAT
                     SNTLNIPSALLDRMEIIRLSGYTEDEKVSIAMQYLVPKQIKANGVKENEAVIEESAVR
                     DIVRYYTREAGVRSLNREIARIARKVVMEQVLAEDKAKTKAAKAKVKAESAQAIVVNA
                     ANLHDYLGVRRFDFGVAESENRIGQVTGLAWTEVGGELLTVEATALKGKGNIIRTGKL
                     GDVMLESITAAMSVVRTRAASVGIAPDFYEKHDMHVHVPEGATPKDGPSAGIAMTLAM
                     VSALTGIPVRADVAMTGEITLRGEVLPIGGLKEKLLAALRGGIKHVLIPKDNVKDLEE
                     IPDNVKQELTIHPVQWIDEVFALGLAETPKIWEEGHEDVGAELPVAKEKSVAKTRSKA
                     TKH"
     gene            1541..1810
                     /locus_tag="PK856_05215"
     CDS             1541..1810
                     /locus_tag="PK856_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRL01802.1"
                     /translation="MNKSELIDAIAQSADISKAAAGKALDGMVAAVTEALKSGDTVTL
                     VGFGTFYVGERAERKGRNPKTGEEITIAAAKSPKFRAGKALKDAL"
     gene            1885..3723
                     /locus_tag="PK856_05220"
     CDS             1885..3723
                     /locus_tag="PK856_05220"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRL01803.1"
                     /translation="MFDIVQKYSTASKIVLGIIGLTFVVAGGVVAGSGAVSNESYLVK
                     VGDQPVTLEKIREIAKQREGEITDQVQKDILTALTQQAYLLEGAKRMGVVVSDEQLKK
                     IIMQQTVFQENGVFKESLYKDYLAKNNLKEEAFLDSLRSDFSLQAVFNLIQSSQIIAD
                     SQITQAIHLFEAPRTIRTFTFSQNQFFDKVDTSDKALQAYYDGHKKDFIQAQGLKFDF
                     IVLNGNDVIKQIQVSDAEAKAYFDKNQASLPAKRQVAHILVAFPQGADDKAKAAAKAK
                     AEEVLAKVKAKPADFAALAKQYSQDPGSAQNGGDLGFFAKDGTMVKPFEDAAFSLKQG
                     EISSLVETQFGYHILKVLEVGQSFESQKANIVQTLQQEKIKTAVDGKKKQLVEAAVKH
                     GGDIAAVAKETGLNVQPVGDWVTESQAKAAQMPEALQKVLFDAKTIKDKKVSEPIDVG
                     QNTYWVLQVKEVRPETQLTLEQAKAGVKEAVLMSEGGKLAAAAAKDALAKLQKGETVD
                     LKWSAAADLTPQQAQQTMPPEAYKALIAISPANGKPAYTMLPGLPAPVLVAVDKIGEV
                     ADQPELHGQMKNILAKNQNDGTLLGLINYLKTQFKEVTGSEKLISE"
     gene            complement(4176..4757)
                     /locus_tag="PK856_05225"
     CDS             complement(4176..4757)
                     /locus_tag="PK856_05225"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRL01804.1"
                     /translation="MEHKLPVLPYALDALEPHLSKETLEYHYGKHHQTYITNLNNQIK
                     GTEFEELSLEDIVKKSSGGMFNNAAQTWNHTFYWLGFTPKGEGKPAGDLAAAIDAKWG
                     SFEKFQEAFNTVAAGTFGSGWAWLVKTPAGELDLVSTSNAATPLTTENTALLTCDVWE
                     HAYYIDYRNSRPNYLKGFWEIVSWAEVAKRFAA"
     gene            4951..>5134
                     /locus_tag="PK856_05230"
     CDS             4951..>5134
                     /locus_tag="PK856_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HRL01805.1"
                     /translation="MTESFDSPDFDLPPDDVMAISLPPHSVEAEQSVLGGLLLENSAY
                     DRIADILTEEDFYRQEH"
BASE COUNT         1451 a         1007 c         1305 g         1371 t
ORIGIN      
        1 tgccaaggcc actggtcgcc aatacgtgcg catggctttg ggtggcgtgc gtgatgaaag
       61 cgaaattcgc ggtcaccgcc gtacctatat tggttctatg ccaggcaaaa ttttgcaaaa
      121 catggccaaa gtgggcgtga aaaacccatt gttcttgctc gatgaaatcg acaaaatggg
      181 ccaagatttc cgtggcgacc ctgccagtgc cttgttggaa gtgttagacc ctgagcaaaa
      241 ccacagtttt gtcgatcatt atgctgaagt ggaatacgat ttgtctgatg tgatgttcat
      301 cgccacatca aataccttga acattccttc tgctttgctc gatcgcatgg aaatcatccg
      361 cttgtcgggt tataccgaag acgaaaaagt cagcattgcc atgcagtatt tggtgcccaa
      421 gcaaatcaaa gccaatggtg tcaaagagaa tgaggctgtg attgaagagt cagcggtgcg
      481 cgatattgtg cgctactaca cccgtgaagc aggtgtgcgt tcattgaacc gtgaaattgc
      541 ccgcattgcc cgtaaagtgg tgatggagca ggtgttggcc gaagacaagg caaaaaccaa
      601 agctgcgaaa gccaaggtaa aagcggagtc ggctcaagcc atcgtggtga atgcggcgaa
      661 tttgcatgat tatttgggcg tgcgccgttt tgatttcggt gtggccgaat cggaaaaccg
      721 cataggccaa gtgacaggtc tggcttggac ggaagtgggc ggtgaattgc tcacagttga
      781 agccacagcc ttgaaaggta agggcaacat catccgcaca ggtaagcttg gtgatgtgat
      841 gctggagtcc atcaccgctg ccatgagcgt ggtgcgcacg cgagctgcct cagtgggcat
      901 tgcaccagat ttctatgaga aacacgacat gcatgtgcac gtgccagaag gggccactcc
      961 caaagatggc cctagtgcag gtattgccat gactttggcc atggtgtctg ctttgacggg
     1021 tattccagtg cgtgcggacg tggcgatgac gggtgaaatt actttgcgtg gtgaagtgtt
     1081 acccataggc ggcctgaaag aaaaattgtt ggcagctttg cgaggtggca tcaaacatgt
     1141 gttgattcca aaagacaatg tcaaagattt ggaagaaatc ccagacaatg tgaagcaaga
     1201 gttgaccata catcctgtgc agtggataga tgaggtgttt gctttgggct tggctgaaac
     1261 cccgaaaatt tgggaagaag gccatgagga tgtcggtgct gaattgcccg ttgcaaaaga
     1321 gaaatctgtt gcaaaaacgc gcagtaaagc gaccaagcac taagttttgt caaacaatgg
     1381 tgtgcgagtg ggtaaaacag tggtaaaaat agcggcaatg ccactcactc acttgacatg
     1441 ctgtttttag gcttgctata aagcgtgtat ggattgagtt gcttaaagct tagagcgata
     1501 atttctatca acgaataaac cacagaaagg gactttcatt gtgaataagt ctgaactcat
     1561 tgatgcaatc gcacaaagtg cggatatttc taaagcagct gctggtaaag ctttggatgg
     1621 tatggttgca gccgttaccg aagccttgaa aagcggtgat accgtaactt tggttggctt
     1681 tggcactttt tatgtgggtg aacgcgctga gcgcaaaggt cgcaatccta aaacaggtga
     1741 agaaatcacc attgctgctg cgaaatcacc aaaattccgc gctggcaagg ctttgaaaga
     1801 cgctttataa tggcatttag ccattaatga aaaggtgaac atttggttca cctttttttt
     1861 cttatttaag gtacgaatac catcatgttt gatattgttc aaaaatactc cactgcttca
     1921 aaaattgttt tgggtatcat cggtttgact tttgtggttg caggcggtgt cgttgctggc
     1981 tctggtgcgg tttcaaatga aagttatttg gtgaaagtgg gggatcagcc tgtcactttg
     2041 gaaaaaatcc gtgagattgc caaacaacgt gaaggcgaaa ttactgatca agtccaaaaa
     2101 gacatattga cggccttgac tcagcaagcc tatttgcttg aaggtgccaa acgcatgggt
     2161 gtggtggtgt ctgatgaaca attgaaaaaa atcatcatgc agcaaaccgt tttccaagaa
     2221 aacggcgttt ttaaagagag tttatacaaa gattatttgg ctaagaacaa tttgaaagaa
     2281 gaggcgttct tagacagctt acgcagcgat ttttccttac aggccgtgtt taacctgatt
     2341 caatccagcc aaatcatcgc tgacagtcaa atcacgcaag ccatccattt gtttgaagcc
     2401 ccacgcacca tacgcacgtt tactttcagt caaaaccaat tctttgacaa agtggatacg
     2461 tcagacaaag cattgcaagc gtattacgat ggacataaaa aagatttcat ccaagcccaa
     2521 ggcttgaaat ttgatttcat cgtgttaaat ggcaacgatg tcatcaagca aatccaagtc
     2581 agcgatgccg aagccaaggc atatttcgat aaaaaccaag caagcttgcc agccaagcgt
     2641 caagtagccc atattttagt ggcctttcct caaggtgcag atgacaaagc caaagctgct
     2701 gctaaggcta aggcggaaga agtcttggcg aaggtcaaag ccaaaccagc agattttgct
     2761 gccttggcga aacagtattc acaagatcca ggctctgcac aaaatggtgg tgatttgggc
     2821 ttttttgcca aagacggcac catggtgaag ccatttgaag atgctgcgtt ttctttgaag
     2881 caaggtgaaa tcagcagttt ggtggaaaca caatttggct accacatttt gaaagtgttg
     2941 gaagtaggcc aaagctttga aagccaaaaa gccaatattg ttcaaactct gcaacaagag
     3001 aaaatcaaaa cagcggttga tggcaagaaa aaacaattgg ttgaggcagc ggtgaaacac
     3061 ggtggcgaca ttgctgccgt ggccaaagaa accggcctga atgtacaacc tgttggtgat
     3121 tgggtgacgg aaagtcaggc aaaagccgct caaatgccag aagctttgca aaaagtattg
     3181 tttgatgcca aaaccattaa agacaaaaag gtatctgagc cgattgatgt gggccagaat
     3241 acctattggg tattgcaagt caaagaagtc cgccctgaaa ctcagctgac tttggagcag
     3301 gctaaagcag gtgttaaaga agcagtgttg atgtccgaag gtggtaaatt ggcagctgct
     3361 gccgctaagg atgctttggc caaattgcaa aaaggcgaaa ctgtggattt gaaatggtcg
     3421 gctgctgcgg atttaacccc acagcaagcg cagcaaacca tgccacctga ggcgtataag
     3481 gcgctgattg ccatcagccc agccaatgga aaacctgctt ataccatgtt gccagggctg
     3541 cctgctcctg tattggttgc agtggataag attggtgagg tggccgatca gcctgagttg
     3601 catggtcaaa tgaaaaacat tttggcgaaa aaccaaaacg atggcacttt gttgggtttg
     3661 atcaattatt tgaaaaccca gtttaaagaa gttactggta gcgagaaatt gatttctgaa
     3721 tgatgttttg attctttgaa taagcggcct gtgtgtacag gccgctttgt tttgggttaa
     3781 caaaagtagg gtaaatatcc tgtagacagg aatgctattt taggcttagt tatgagagtg
     3841 gcagtttcat gatgagtaaa ccaattgatg gttgcgtaca taatcacata atgatgaact
     3901 agaagattgt tatttggtat gaagattgtt atttggtata tagaagtcgt gttttgtaag
     3961 tcgtttggcc tgttattagc catctgatta aatgtgatgg gaactgagta atgatgacag
     4021 ttatagttaa gtggaacatt taatgtgtgc acacccaaat attatatttg ttcgagtcgc
     4081 ctaaagctga cgaataaggc ttgggtgggt tatatgagag ataaaaaaag cggcctgtaa
     4141 acttacaggc cgcttggtat tactgagttc aggaattaag ctgcgaagcg ttttgcaact
     4201 tcggcccagc ttacgatttc ccagaagcct tttaaatagt taggacggct gttgcggtag
     4261 tcgatgtagt aagcgtgttc ccacacatcg caagtcaaca atgcagtgtt ttcagtggtc
     4321 aatggggtag ccgcattgct ggttgaaacc aagtccaatt cgccagcagg ggttttcacc
     4381 aaccaagccc agcctgaacc aaacgtacca gcagcaacgg tgttgaaggc ttcttggaat
     4441 ttttcaaaag agccccattt tgcgtcaatt gcagcggcca aatcgccagc aggtttacct
     4501 tcgcctttag gcgtgaaacc caaccagtag aaggtatggt tccaagtttg tgctgcgttg
     4561 ttgaacatgc cgcctgaaga ttttttcacg atgtcttcca aagacaattc ttcgaattca
     4621 gtgcctttga tttggttgtt cagattggtg atgtaggttt gatggtgttt accgtagtgg
     4681 tattccaatg tttctttaga caaatgaggt tccaaagcat ccaaagcata gggtaaaact
     4741 ggtaatttgt gttccatgtg tgactccata tcatccatca ttaatcaatt aaacatattt
     4801 tttatgttgt gtgagacatt ctactgtgtt tttcgggata aagtaagccg cagccttggg
     4861 ccatagtttg tgtataagta gcgatagtca gtgattcaag cacttgccaa acaatgttaa
     4921 aatgcttggg tatttgttta ggaatgtcac atgactgagt cttttgattc ccctgatttt
     4981 gatttgccgc ccgatgatgt aatggcgatt tcgttgccac cgcactctgt tgaagccgag
     5041 cagtcggtat tgggcggctt attattggaa aacagtgcgt atgaccgcat tgccgatatt
     5101 ttgaccgaag aagattttta ccgccaagag cacc
//
LOCUS       DAOMVK010000116         5121 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_4533_length_5121_cov_3.454599, whole genome shotgun sequence.
ACCESSION   DAOMVK010000116 DAOMVK010000000
VERSION     DAOMVK010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4533_length_5121_cov_3.454599"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(226..1971)
                     /locus_tag="PK856_05235"
     CDS             complement(226..1971)
                     /locus_tag="PK856_05235"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HRL01806.1"
                     /translation="MATLTQHHFISHDGVSLFYQHQAATAPTATKKAIVLFHRGHEHS
                     GRILHVAEELVLDDCDFFAWDARGHGMSPGERGDAPSFGAMVADVDAFIRHIQTTHNI
                     AEENIIVVAQSVGAVVVATWLHDYAPNVRGAVLTSPAFKVKLYVPFAQSGLKLMQKMR
                     GNFFVQSYVKAHYLSHDPERIHSYQNDDKITRAISVRILLGLYQAAQRVVKDAAAITT
                     PIQLLISGRDWVVHHGPQHDFYNHLGSHIKERIVFDGFYHDTLGEKDRELAIDAMRRF
                     IRKLYEMPPQHIDLTQAHIHGHSKREADELSTPLSPLSARGLYWNGQRAGISLGAKWS
                     EGLKLGKETGFDSGSTLDYVYRNTPTGSNGFGRMADKNYLNAIGWRGIRQRKQNLQTL
                     IQDAMQRLREQGQPVHVMDIAAGHGRYVLDALTAQEQPDSIRLRDYSPLNVEAGRKLI
                     QERQLQDIASFDEVNAYERANYQNLDPQPTLGIVSGLHELFPDNDLINQSLLGFADAI
                     ASGNYLIYTNQPWHPQLEMIARCLTSHRQGEAWVMRRRSQREMDQLVAAAGFDKVRQL
                     IDEDGIFTVTLARKR"
     gene            complement(1974..2582)
                     /locus_tag="PK856_05240"
     CDS             complement(1974..2582)
                     /locus_tag="PK856_05240"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRL01807.1"
                     /translation="MSVYALKPKFQALLRPMVQRLAQRGINANQVTLLACVLSIVMGL
                     LISLCLFYGWFWGLLLLPIFLFVRMAMNAIDGMLAREHAQQSALGGYLNELTDVVSDA
                     ALYLPLLLLPDSSSFLVWLLVLQAALSEFAGVLGQVHGNGRRYDGPMGKSDRALWLGL
                     FAIIWFAFPQTLAYSNLVFLIINLALTYTIYRRVRNGLRPQE"
     gene            complement(2751..4049)
                     /gene="aceA"
                     /locus_tag="PK856_05245"
     CDS             complement(2751..4049)
                     /gene="aceA"
                     /locus_tag="PK856_05245"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HRL01808.1"
                     /translation="MTTRAERIAAIEKDWAENPRWKGITRDYSAEDVERLRGSVQVEH
                     TIARQGAEKLWTLVNNEDFINCLGALTGGQAMQQVKAGIKAIYLSGWQVAADNNGYMS
                     MYPDQSLYPVNSVPAVVERINNAFARADEIQHAKGVEKGDADYIDYYAPVVADAEAGF
                     GGVLNAFELMKSMIKAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLTAARFAA
                     DVAGVPTVLLARTDANAADLLTSDYDENDRPFVTGERTAEGFYRVNAGIEQAISRGLA
                     YAPYADLLWCETAKPDLEEARQFAEAIHAKFPGKLLAYNCSPSFNWKKNLDDATIAKF
                     QRELGAMGYKYQFITLAGIHNMWYNMFDLAQDYAERGMSAYVEKVQEPEFAARDRGYT
                     FVSHQQEVGTGYFDDVTTVIQGGASSVTALTGSTEEEQFH"
     gene            complement(4975..5115)
                     /locus_tag="PK856_05250"
     CDS             complement(4975..5115)
                     /locus_tag="PK856_05250"
                     /inference="COORDINATES: protein motif:HMM:NF018067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01809.1"
                     /translation="MLTRQFWQSRKDRIESGIIEDFFPYPQSTRFYNDGDIYDSHTES
                     DA"
BASE COUNT         1298 a         1239 c         1328 g         1256 t
ORIGIN      
        1 ctgcgtaaaa cagattcagc gtccaatacg gcacgatgct ccaagcccaa aacggcgtgt
       61 ggcgctccca cggccaaaaa atttcaggca cgttgtcgcg caagcctgcc aaataattgg
      121 cgccgccata cgtgctgtaa aacaccatac ccatgcccag caaacacagc aggcaatagc
      181 accaactgag cttaggaggg gtgtcaaagc agtttacagg ccgcatcagc gtttgcgcgc
      241 caaggtcacg gtgaagatgc cgtcttcgtc tatcaattga cgcaccttat cgaaacctgc
      301 ggcggcaacc aattggtcca tttcgcgttg gctgcggcga cgcatcaccc acgcttcgcc
      361 ttgcctgtgg ctggtgaggc agcgcgcaat catttccaat tgaggatgcc acggttgatt
      421 ggtgtaaatc aaataattgc cgctggcgat ggcatcggca aagccaagca aagattggtt
      481 gattaaatcg ttgtcgggaa acaattcatg taagcccgac acgataccta atgtcggctg
      541 tgggtcgagg ttttggtaat tggcgcgctc gtaagcgttc acctcatcaa agctggcaat
      601 gtcttgcaat tggcgctctt gtatcagctt gcggcctgct tcaacattga gcgggctgta
      661 atcgcgcaaa cggatggaat cgggttgctc ttgtgcggtt aaggcatcga gcacatagcg
      721 gccgtggcct gcggcgatgt ccatcacatg cacaggctga ccttgctcgc gcaagcgctg
      781 catcgcatct tgaatgaggg tttgcaggtt ttgcttgcgc tgacgaatgc cgcgccaacc
      841 gatggcattc aaatagtttt tatccgccat gcgcccaaag ccattgctgc ctgtgggcgt
      901 gttgcggtag acataatcca aggtagagcc tgaatcaaag ccggtttcct tgcctaactt
      961 caaaccttcc gaccatttcg cacccaaaga aatgcctgcg cgttggccat tccaatacag
     1021 gccgcgcgca gacaaaggcg acagcggcgt ggacaattca tcggcttcgc gtttgctgtg
     1081 gccatgaatg tgggcttggg taaggtctat gtgttgcggc ggcatctcgt acagtttgcg
     1141 tatgaagcgg cgcatggcat cgatggccag ttcgcggtct ttttcgccca aggtgtcgtg
     1201 gtaaaagcca tcaaacacaa tgcgctcttt gatgtgcgaa cccaaatggt tgtaaaaatc
     1261 gtgttgcggg ccatggtgta ccacccaatc gcggcctgaa atcagcaatt gtattggcgt
     1321 ggtaatcgct gccgcgtctt tcaccacacg ctgcgcggct tgatagaggc cgagtaaaat
     1381 gcgcaccgaa atggcgcggg tgattttgtc atcgttttgg tagctgtgta tgcgctcggg
     1441 gtcgtggctg aggtaatggg ctttaacata gctttgcaca aagaaattgc cacgcatttt
     1501 ttgcatcagc ttcaggccgc tttgggcaaa tggcacatac agttttactt tgaatgcagg
     1561 cgaagtcagc actgcgccgc gaacattggg cgcgtaatcg tgtagccaag tggccaccac
     1621 cactgcaccc acgctttgcg ccaccacgat gatgttttct tcggcgatat tgtgagtggt
     1681 ttgaatgtgg cggatgaagg catcgacatc ggccaccatc gcgccaaaac tgggcgcatc
     1741 gccgcgctcg cctgggctca tgccgtggcc gcgcgcatcc caagcaaaga aatcacaatc
     1801 gtccaatacc aattcttccg ccacgtgcaa aatgcggcct gaatgttcgt gcccacggtg
     1861 aaacaacacg atggcttttt tggtggcagt gggggctgtg gcggcttggt gttggtaaaa
     1921 caaagacacg ccatcgtggc tgatgaaatg gtgttgggta agggtggcca taatcactcc
     1981 tgtgggcgca agccgttgcg cacgcgccga taaatggtat aggtcaaagc gagattgata
     2041 ataaggaaga ccagattgct gtaagccaaa gtctgtggaa aggcaaacca aatgatggca
     2101 aacaagccca gccacagcgc tctatcactt ttgcccatgg ggccatcgta gcggcggccg
     2161 ttgccatgca cttgccccaa tacccctgca aactcgctta atgcggcctg taaaaccagc
     2221 aaccagacca aaaaagagct gctgtcaggc aacagcaata agggcagata caaggcggca
     2281 tcggaaacca catcggttaa ttcgtttaaa tagccgccca atgccgattg ctgcgcgtgt
     2341 tcacgcgcga gcataccatc gatggcattc atcgccatgc gcacaaacaa aaaaattggc
     2401 aacagcaaca agccccagaa ccagccataa aacaagcaca gactgatgag caaacccatc
     2461 acaatggaca gcacacaggc gagcagcgtc acttgattgg cattgatgcc gcgctgtgcc
     2521 aagcgctgca ccatggggcg cagcaaggct tgaaatttgg gttttaaggc gtacacgctc
     2581 atggtttgct cttgattggg gaggctgtct tgacagacat catcatgatt ttggtcaaca
     2641 cttgtcaatg ccattgcact catgcacacc caagtatcag aaagtgcgcc atcagagaac
     2701 aaaaaaggca acccgagggt tgcccaaaac gacacacatt atttggagta tcagtggaac
     2761 tgttcttctt cggtagaacc ggtcaaagcc gtcacacttg atgcgccgcc ttgaatcacg
     2821 gtggtcacat cgtcgaagta gccagtgccc acttcttgtt ggtgcgatac aaaggtatag
     2881 ccacggtcgc gtgctgcgaa ttcaggctct tgtacttttt caacataagc cgacatgccg
     2941 cgttcagcgt agtcttgagc caagtcgaac atgttgtacc acatgttgtg aatgccagcc
     3001 aaagtgatga attggtattt gtaacccatc gcacctaatt cgcgttggaa tttggcaatg
     3061 gtcgcgtcat ccaagttttt cttccagttg aaagaaggtg aacagttgta agccaacaat
     3121 ttgcctggga atttggcatg aatcgcttct gcaaattgac gcgcttcttc caaatcaggt
     3181 ttggcagttt cacaccacaa caagtcagca taaggagcgt aagccaagcc acgagaaatc
     3241 gcttgctcga tgcctgcgtt aacgcgatag aagccttcag cagtgcgttc acccgttaca
     3301 aatggacggt cgttttcatc gtaatcgctg gtcaacaaat cagcggcatt ggcatcggta
     3361 cgcgccaaca acaccgtagg cacacctgct acgtcagcag caaaacgcgc tgcagtcaat
     3421 ttttgaatcg cttcttgggt aggcaccaat actttgccgc ccatgtggcc acattttttc
     3481 acagaagcca attggtcttc aaagtgaacg cccgccgcgc ccgctttgat cattgatttc
     3541 atcaattcaa acgcgttcaa cacaccgcca aaacctgctt cagcatccgc aaccactgga
     3601 gcgtagtaat cgatgtagtc tgcgtcacct ttttcaacgc ctttggcgtg ttggatttcg
     3661 tcagcacggg caaaagcatt gttgatgcgt tccacgacag cagggacaga gttcacaggg
     3721 tagagcgatt ggtctgggta catgctcatg tagccgttgt tatcggcggc cacttgccag
     3781 cctgataagt aaatcgcttt gatgccggct ttgacttgtt gcatggcttg accaccggtc
     3841 aatgcgccca agcagttgat gaagtcttca ttgttcacca aagtccacaa tttttccgcg
     3901 ccttggcggg caatggtgtg ctcaacttgt acggaaccac gtaaacgctc cacatcttca
     3961 gcgctatagt cgcgagtgat gcctttccag cgaggatttt ctgcccagtc tttttcaatg
     4021 gctgcaatgc gttctgcacg tgttgtcatg gtaattcttc ctttgttcta atcggagttt
     4081 ggaaatctgc acacggtggt gcgatgcagc aaatttaacc actgtcttgg gaaaaaagca
     4141 aagggttttt ttaaaaaaat ggcttatttt cagcatcttg tgaaacgaaa aagcgaaaat
     4201 ttggatatgc tggattgcaa caatttccaa cattttttaa tactgtttgc atgaataatt
     4261 gcaaatgaac acatttcaac acgaacctat gagaatgatt gttcgcattt gttcgcaaag
     4321 agtggggaat gatacaaatc gaaacgcgag aataatgtgg atttcaccac aaatggataa
     4381 aaattcagct taaaaaatgc tgcgatgcgg tgttatttgt cacaataaac gtgcgtgaaa
     4441 gcctgtattt tggtttctgg ggtggtgttt ttggatgtat tggcatgtag tccgcaattt
     4501 tatggttgaa aatcatgcgg ctttctgcga tttatctagc agcgcgcaat aaatgctgca
     4561 cctgcttaca ggccgcttgt attgcggttc aatcaaaata atggatttaa ggactatatt
     4621 cacacgtcta tccagtccaa gcgggctgga tggctgtggt acgactgcta taaaacagaa
     4681 aagctagcgg tgctaggcgt taatgtgaag gagggtaaag accatcagtt aaggcacgga
     4741 cagggttggg agggttaaga aggcaattga agtttggaag tctgtgccat gaactagacg
     4801 gtgtggcttg gggaagtacg gaattcagcc aaattgaagg atgtttcggt acgagttaag
     4861 tgaatctgag tttacaggcc gcatgaagcg gcctgtaaat agatggttaa ctgatgatgt
     4921 gatgacacaa caatctttat agtggattgc aatacacaaa cagtgtatca gccatcatgc
     4981 atcgctttcg gtgtggctgt cgtaaatgtc gccatcgttg taaaagcggg tggattgtgg
     5041 ataagggaaa aagtcttcaa tgatgccgct ttcgatgcgg tctttgcgcg attgccaaaa
     5101 ctggcgcgtg agcaaatcgc g
//
LOCUS       DAOMVK010000117         5099 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_4568_length_5099_cov_3.082276, whole genome shotgun sequence.
ACCESSION   DAOMVK010000117 DAOMVK010000000
VERSION     DAOMVK010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4568_length_5099_cov_3.082276"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /gene="mnmA"
                     /locus_tag="PK856_05255"
     CDS             <1..332
                     /gene="mnmA"
                     /locus_tag="PK856_05255"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959315.1"
                     /note="catalyzes a sulfuration reaction to synthesize
                     2-thiouridine at the U34 position of tRNAs; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRL01810.1"
                     /translation="PGEPWFVADKDLEKNELIVVQGHDHLLLQSRSLRMHDLSFTLPT
                     RPQEGVYTCKTRYRMQDAVCELHYIDDKNIELRFKEPQWAVTPGQSAVLYDGDVCLGG
                     GIITSRA"
     gene            380..2059
                     /locus_tag="PK856_05260"
     CDS             380..2059
                     /locus_tag="PK856_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRL01811.1"
                     /translation="MEKIWLSQYDEGVPEYVDVTAYSSISAVFNESAAQYGQRPAFTN
                     MGKTLTYAETKQYTDQFSAYLQHVLKLPKGERVAIMMPNVLQYPIAVFGALQAGYVVA
                     NVNPLYTPRELEHQLVDCGATTIVVLENFANTLGLVLKNTPVKNVIIANIGEMLGGVK
                     GTLVNFVLRHVKKMVPAYNIPNTISFKDTLAQGKNHTFTPVELTLEDVAFLQYTGGTT
                     GVAKGAVLTHGNIVANMQQAGAWIKAKLSRDGETILTALPLYHIFCLTVNLMIFTKIG
                     GHNILITNPRDIPALVKEIAKYRVAVITAVNTLFNALVNNADFAHVDFSKLKLSVGGG
                     MAVQKAVADKWQKITNQPIIEAYGLTETSPGVCVNPLSSTSYSGNIGLPISNTEIDVR
                     DGEGKSVGIGEAGELFVRGPQVMRGYWNMPEETQKVLGADGFLSTGDVVIVNEQGFVK
                     IVDRKKDMIVVSGFNVYPNEIEDVVAGHPGVLEVACVGVPSDKTGEAIRLFVVKKDPA
                     LTKEDLIAFCRENLTGYKVPKDIVFKNELPKSNVGKILRRELKDAPAEDHK"
     gene            2302..3480
                     /locus_tag="PK856_05265"
     CDS             2302..3480
                     /locus_tag="PK856_05265"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL01812.1"
                     /translation="MLANSKIPPELSIVLILTFGAFSLGMAEFTTIWLLPEMAHSLDT
                     TTAKIGPAASFYALGVLFGTPIVALLGSSLPRQKLLTYMLMWCFAGNLATSFVTSYEQ
                     LKWVRLFSGVPHGVFLGVAGVIFGQMLPADKLGKAIGFMMAGIGMALLMIVPLTTYFG
                     EIQDWRAIFRVIALMDLLIIILLHDLIPDLPTQAAQSGWREFKTLKNPLLWWILALST
                     TAICGRMAIFAFAGPLVTEVALLPATKLPLIVICGGIGTVVGFLLGGYFADKNALKTL
                     WYSLLWCLLVMVVFQMLIPHTHLNYLAFFLLGTFMLIVPSLQVLSMRYAPEGQTFASC
                     LNHAAMNGANAAGPILAGVFIGAGYGWLSVTWVGIIIGVLALLIYVIGRFTLMQNTPS
                     "
     gene            3590..4372
                     /locus_tag="PK856_05270"
     CDS             3590..4372
                     /locus_tag="PK856_05270"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRL01813.1"
                     /translation="MSQPPKHFWHNPNWPELETRSAQNSHACYRMHSHRAHSFGCVDA
                     GVTVLSFADGSAIPLQAGDVLWLPAQLPHCCNPPSQQHWAYQMMYLTPSLLAPNLPNT
                     DKIHIWRETELYQRFQHLHQVLQSNRPLTDKQAQLQAVLHDVFAQASYPVLWQHQPPA
                     QACDDRLLNLVQHPDFLLWNVAQMARHVHMSRYHFMHWFRALTGWSAHAYQVNLRIEQ
                     ARLGLRTGSPIAHLAHDLGFADQSHFQRVFKAHTAATPKQYQ"
     gene            4447..5076
                     /locus_tag="PK856_05275"
     CDS             4447..5076
                     /locus_tag="PK856_05275"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HRL01814.1"
                     /translation="MELFLLVIGSHFLALLLPGADFFLIVRLATRSGWRACLPLCTGI
                     ALGNACYILLAFTGLQVLQKIPALLWSIQTLGAAYLLYLSFLLLRPKPPQTFQSTMPI
                     QIQNTSKRQFGMGLFSALLNPKNGLFYMTLASVLGQQQASLGLYVVCGLWMFSMVWLW
                     DMLVAVLLGNPLLLRRFQTCLPLIERGAGIMLALLAAVMLWPILQTWLR"
BASE COUNT         1225 a         1341 c         1214 g         1319 t
ORIGIN      
        1 gccctggtga gccttggttt gtggccgaca aagatttgga aaaaaatgag ttgatcgtgg
       61 tgcaaggcca cgaccatcta ttattgcaat cgcgctcttt gcgcatgcat gatttgagct
      121 tcaccttacc aacacgccca caagaaggcg tatacacgtg taaaacccgc tatcgcatgc
      181 aagatgcggt atgcgaattg cattacattg atgacaaaaa catcgaattg cgctttaaag
      241 aaccacaatg ggcagtgaca cctggtcagt ctgcggtgct ttacgacggc gatgtgtgct
      301 tgggtggtgg catcatcacc agccgcgctt aagcctctgc tgccacaata aaatcaaaac
      361 actttaggag aaagacgcta tggaaaaaat ttggttatca caatacgatg aaggcgtacc
      421 cgagtatgtc gatgtcacag cctattcttc catcagcgcc gtgttcaacg aaagtgcagc
      481 ccaatacggc caacgtcctg cgtttaccaa catgggcaaa accctgactt acgcggaaac
      541 caaacagtat accgaccaat tttctgcgta tttgcaacat gtgttgaagt tgcccaaggg
      601 cgagcgcgtc gccatcatga tgccgaatgt gttgcaatac cccatcgctg tgtttggcgc
      661 tttacaggcc ggttatgtgg tggcaaacgt caatcctctg tatacgccgc gcgaattgga
      721 acaccaattg gtcgattgtg gcgccaccac catcgtggtg ttggaaaact ttgccaatac
      781 gctgggcttg gtgttgaaaa acacgcctgt taaaaacgtc atcattgcca acatcggcga
      841 gatgctcggt ggcgtgaaag gcacgctggt gaattttgtg ctgcgccatg tcaaaaaaat
      901 ggtgcctgca tacaacattc ccaacaccat ttcattcaaa gacaccttgg ctcaaggcaa
      961 aaaccacacc tttacacctg ttgaactcac gctggaagat gtggccttct tgcaatacac
     1021 tggcggcacc acaggcgtgg ccaagggtgc cgttttgacc cacggcaaca tcgtcgccaa
     1081 catgcagcaa gcaggcgcat ggattaaggc caagctctcg cgcgatggcg aaaccatcct
     1141 caccgccttg ccgctgtatc acattttttg cttaaccgtg aacctgatga ttttcaccaa
     1201 aatcggcggc cacaatattt tgattaccaa tccgcgcgac attcccgcct tggtcaagga
     1261 aatcgccaaa taccgcgtgg cggtgattac cgcagtgaac accttgttca atgccttggt
     1321 gaacaatgcc gattttgctc atgtggattt ttccaagcta aaactgtcag tcggtggcgg
     1381 catggcggtg caaaaagctg tggctgacaa atggcaaaaa atcaccaacc aaccgattat
     1441 tgaggcttac ggcctcaccg aaaccagccc aggtgtgtgt gtgaacccgc tttcttctac
     1501 ctcttactca ggcaatatcg gcttaccgat ttccaatacc gaaatcgatg tgcgcgatgg
     1561 tgaaggcaaa tcggtgggca taggtgaagc tggcgaattg tttgtgcgtg gcccacaagt
     1621 gatgcgcggc tattggaaca tgccagaaga aacccaaaaa gtcttgggcg cagacggctt
     1681 cttgtccaca ggcgacgtgg tgatagtgaa tgagcaaggt tttgtgaaaa ttgtcgatcg
     1741 caaaaaagac atgattgtgg tgtctggctt caatgtctat cccaatgaaa ttgaagacgt
     1801 ggtggctggc caccctggcg tattggaagt ggcttgtgtg ggcgtgccca gcgacaaaac
     1861 tggcgaagcc atccgtttgt ttgtggtgaa aaaagacccc gccctcacca aagaggattt
     1921 aatcgcgttt tgccgcgaaa acctcacggg ttacaaagtg cccaaagaca tcgtgtttaa
     1981 aaatgaatta cccaaatcca atgtgggtaa aattctccgc cgcgaattaa aagacgcgcc
     2041 tgctgaagac cacaaataac ctgaaccgtt tgtatttaca tatttacagg ccgataacat
     2101 cggcctgtaa gctttttacc catgtcattg caaagaaaat caaaccctcg ttttgaagca
     2161 aattcaatca cagaaacact tacaagctgt ttgaagcata agcactatag aaccattggt
     2221 tttaattgat tgccctaatc gaaatcaatc gtcataatta gccgcaaaac gctgattgaa
     2281 ttgatttcct gagtccttcc tatgttagca aattccaaaa tcccgcccga attgtcgata
     2341 gtactcatcc tcactttcgg ggcattctct ttgggcatgg cagagttcac caccatatgg
     2401 ttactgcctg aaatggccca cagtttggac accaccaccg caaaaattgg ccctgcggcc
     2461 agcttttatg ctttaggggt gttattcggc acgcccatag tggccttact tggctcgagc
     2521 ctaccgcggc aaaaattgct cacctacatg ctgatgtggt gtttcgctgg caacctagcc
     2581 acctcatttg tgacttctta tgaacaactg aagtgggtac gtttgttcag cggcgtgcca
     2641 catggcgtgt ttctaggggt ggcaggagtc atctttgggc aaatgctgcc tgcagacaag
     2701 ctgggcaaag ccatcggctt catgatggca ggcataggca tggccttact catgattgtg
     2761 ccgctgacca cttattttgg tgaaattcaa gattggcgcg ccatctttcg ggtgattgca
     2821 ttgatggact tgctcatcat catcttattg cacgatttga ttccagactt gccaacacaa
     2881 gcggcccaat cgggttggcg tgaattcaaa accctcaaga atcctttgtt gtggtggata
     2941 ctcgcattga gcaccactgc catttgtggg cgcatggcca tttttgcctt tgctggccca
     3001 ttggtcaccg aagtagcctt actgccagcc acaaaactgc cgttgattgt gatttgcggc
     3061 ggcatcggta cggtggtggg ctttttgttg ggcggctatt ttgccgataa gaatgccctc
     3121 aaaaccctgt ggtattcctt attgtggtgt ttattggtca tggtggtgtt tcaaatgctc
     3181 atcccccata cccacctcaa ctacctcgcc tttttcttac tgggcacctt catgttaatc
     3241 gtgccctcgc tgcaagtatt gagcatgcgc tatgcaccgg aaggacaaac ctttgcctca
     3301 tgcctgaacc atgccgcgat gaatggtgcc aatgccgctg gccccattct ggcgggcgtg
     3361 tttatcggag cgggatatgg ttggctgtcg gtcacttggg taggcatcat catcggggtc
     3421 ttggctttgc tgatttatgt catcggccgc tttaccctga tgcaaaacac accatcttaa
     3481 agcctttgtt cgcattttta agcccatctc aacggcacat gtttacaggc cgcttcaacg
     3541 gcctgtattc tcattctatt tcaatgcggc acggaaagcg aatactggta tgtcacagcc
     3601 cccaaagcat ttttggcaca atccaaactg gcctgaattg gaaacccgca gcgcccaaaa
     3661 cagccatgct tgctaccgca tgcacagcca ccgcgcccac tcatttggct gtgtggatgc
     3721 gggtgtgacc gtattgtcgt ttgctgatgg cagcgccatt cccctgcaag caggcgatgt
     3781 tttgtggctg cctgcccaat taccacattg ctgcaaccca ccgtcgcagc agcattgggc
     3841 ttaccaaatg atgtatctca ccccgagctt gctggctcca aacctcccaa ataccgacaa
     3901 gatacacatt tggcgcgaaa ccgaactcta ccaacgcttt caacacctgc accaagtctt
     3961 gcaatccaat cggcctttga cagacaaaca agcccaactc caagccgtct tacacgatgt
     4021 gtttgcccaa gcaagctatc ccgtgttgtg gcaacaccaa cccccagccc aagcatgcga
     4081 tgaccgcttg ctgaatttgg tgcaacatcc tgattttctg ttatggaatg tggcgcaaat
     4141 ggcccgccat gtgcacatga gccgctacca tttcatgcac tggtttcgcg ccctcaccgg
     4201 ctggtctgcc catgcctacc aagtcaatct gcgcatagag caagcccgct tgggtctgcg
     4261 cacaggaagc cccatcgccc accttgccca cgatttgggc tttgccgacc aaagccattt
     4321 ccaacgtgtg ttcaaagccc ataccgctgc cacacccaag caataccaat aaaagcgcac
     4381 ttttcttcaa tacccaaagc aataccatga ggcatgctgt gatttcaaca atcatgggtg
     4441 cggtttatgg aattgttctt actcgtcatc ggcagtcatt ttttggcgct gttactgcca
     4501 ggcgcagatt ttttcttgat tgtgcgtctg gccacacgct caggttggcg cgcgtgtctg
     4561 cccttgtgca ctggcattgc gctgggcaat gcctgctaca tcctcttggc attcacaggt
     4621 ttgcaggttt tgcaaaaaat acctgctttg ctatggagca tacagacatt gggtgctgcc
     4681 tacttgctgt atttaagttt tttgctgttg cggcccaaac caccccaaac ctttcaatcc
     4741 accatgccca ttcaaattca aaacacatcc aaacgccagt ttggcatggg cttgttttct
     4801 gccttgctta accccaaaaa cggcttgttt tacatgacac tggccagtgt tttggggcaa
     4861 caacaggcca gcttgggctt gtatgtggtg tgcgggctgt ggatgttcag tatggtgtgg
     4921 ttatgggaca tgttggtggc cgtattgctt ggcaatcctc tgctcttgcg ccgctttcaa
     4981 acctgtttgc cactgataga gcgcggcgca ggcatcatgt tggccttatt ggcggcagtc
     5041 atgctgtggc ccatcttgca aacttggctg cggtaaaaac aagcagcctg taaatgttt
//
LOCUS       DAOMVK010000118         5091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_4576_length_5091_cov_3.383836, whole genome shotgun sequence.
ACCESSION   DAOMVK010000118 DAOMVK010000000
VERSION     DAOMVK010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4576_length_5091_cov_3.383836"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="PK856_05280"
     CDS             complement(<1..717)
                     /locus_tag="PK856_05280"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HRL01815.1"
                     /translation="MGDVQGCCDELLKLLSKIAFNFGTDTLWLTGDLVNRGPKSLEVL
                     RFAMKHDYCVQTVLGNHDLHLLAVAYGGARLKKGDTLDPILNAVDNKVMLEWLRHQPM
                     FVAHNHYAMVHAGLLPDWDIDQAHDHAEQIEQILVSDRFEWLLQNMYGNKPKQEADIS
                     SELDRLRFSLNVMTRMRAITAQHELDFDFKGEIDDLPENLTPWFAAEHPAWADHKIVF
                     GHWSALGLHVDEHVIGLDTGA"
     gene            complement(934..2010)
                     /gene="prfA"
                     /locus_tag="PK856_05285"
     CDS             complement(934..2010)
                     /gene="prfA"
                     /locus_tag="PK856_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRL01816.1"
                     /translation="MKASLLEKLHQLADRLEEVTHLLASENATDDMDEYRKLNKEYAE
                     LTPLVETYQAFNQAQQDKIDAEEMLSDPDMKEFAAEEINAANDKIETLELDLQKLLLP
                     KDQDDDKNVFIEVRAGTGGDEAALFAGDLLRMYVRYAERQGWKTEMVSESESDLGGYK
                     EAIVRIMGTGVYSQLKFESGAHRVQRVPATESQGRVHTSACTVAVMAEADELEDIAIN
                     AADIRIDTFRASGAGGQHINKTDSAIRITHFPTGLVVECQDGRSQHSNKAQAMKVLAA
                     RLNDLQRREAHEKEAAERKSLVGSGDRSERIRTYNYPQGRVTDHRINLTLYKLDFIMD
                     GDLSEVTGSLIAEHQAELLASISD"
     gene            complement(2096..3346)
                     /gene="hemA"
                     /locus_tag="PK856_05290"
     CDS             complement(2096..3346)
                     /gene="hemA"
                     /locus_tag="PK856_05290"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HRL01817.1"
                     /translation="MQLTAIGLNHQTAPISIRERLAFAAQVLPEAVKQIVQKDAAREA
                     VILSTCNRTELYCVGQSSDIVGWLADFHRLPEDDIRPYLYTLDSDETIRHAFRVSCGL
                     DSMVLGEPQILGQLKDAVRVAQEQSSIDTTLNTLFQKTFSVAKEVRSNTAVGNSSVSM
                     AAASVKMAEQIFPKIADLNVLFIGAGEMIELVATYFSAKSPKKITVANRTLARARELC
                     AQMGGRTDAVLLNDLPGIIHEYDVIVSSTASPLPIVGKGMIESALKQRRRHPMFLLDL
                     AVPRDIEPEVGKIEDAYLYTVDDMVKVVEMGKEARQQAAAEAEAIIDTRVHEFLAWQR
                     ERQIVPMIKALRNEGETQRQQALATALKQLHKGVSAEEVLEKLSLQLTNKLLHAPTVA
                     LHQINQRPELSDAVSSIYRLPEHL"
     gene            3480..4115
                     /gene="pdxH"
                     /locus_tag="PK856_05295"
     CDS             3480..4115
                     /gene="pdxH"
                     /locus_tag="PK856_05295"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HRL01818.1"
                     /translation="MDLHEVRQDYAKQSLELSDCTDLPLPQLEKWLQAAIHAQVHEPT
                     AMNVATVDENGRPNARIVLLKELNEQGLVFFTNYLSRKGRALTEKPFAAVTFFWPELE
                     RQVRVEGRVVKLDEAASDEYFASRPYASRIGAWASEQSQVIPDKAHLMKRAASIGIKH
                     PLSVPRPPHWGGFVVIPDRIEFWQGRPSRLHDRVQYRLQATGEWVKERLAP"
     gene            4253..5002
                     /locus_tag="PK856_05300"
     CDS             4253..5002
                     /locus_tag="PK856_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01819.1"
                     /translation="MKQHLLGVALLAVSASAWSLSCTPKPAFEVRHTANVYTNSVFWL
                     QSSVSESEAPISVADLQLQLDGKAVAALQANPQKIAEYTGFQVQPQLQAGGAYQVVLK
                     DKKRDNYAFLSDLANRFTVETKIVTSPDLAWRESPKLIKVRHHSGTWGDSGNVLWSLK
                     TTRSLEDYLVVIQLAPERNMEKVQTFIVTPVSLEGQEVVGVGFGPCQYGPETYVFSKN
                     STVWATFDLLSHDGKRIPWQGEPQKTKLTPR"
BASE COUNT         1186 a         1252 c         1342 g         1311 t
ORIGIN      
        1 agcgcctgtg tccaagccta tgacgtgctc gtccacatgc agacccaaag ccgaccaatg
       61 accaaacaca atcttgtggt cggcccaagc tggatgctcg gctgcaaacc atggtgtaag
      121 gttctcaggc aagtcgtcga tttcgccttt gaagtcaaaa tccaattcgt gctgtgcggt
      181 gatggcgcgc atgcgtgtca tcacattgag gctgaagcgc aagcggtcta attcgctgct
      241 gatgtcggct tcttgtttgg gtttgttgcc atacatgttt tgcaacagcc attcaaagcg
      301 gtctgacacc aaaatttgct caatttgctc ggcatgatcg tgtgcttggt ctatgtccca
      361 atcgggcaac aaacctgcat gtaccatcgc ataatggttg tgggccacaa acatcggctg
      421 atggcgcagc cattcgagca tgactttgtt gtctaccgca ttcaagatgg ggtcgagggt
      481 gtcgcctttt ttgaggcggg cgccgccata agccacggcg agtaaatgca aatcatggtt
      541 gcctaaaaca gtttgcacgc agtaatcgtg cttcatggcg aagcgcaaga cttctaaaga
      601 tttgggccca cggttgacca agtcacccgt caaccacaaa gtatcggtac caaaattaaa
      661 cgcaattttg gacaaaagtt ttaacaattc atcgcagcaa ccttgtacgt cgcctattgc
      721 ataattgcca tgatgggatt ccttttattc tcgttgtcag ataaccatat accgattgaa
      781 ctctaaacgc aaacctgagc gataaaagtg tgttgcagtg cgtaaatgga gggcggttat
      841 ggtggcgaaa accagtgtag aaaccagaaa aaacaagcgc cctgacagtt tacagggcgc
      901 ttgaagacaa tatgatgatg acaaatatgg gtgttaatcg ctgatggagg cgagcaattc
      961 tgcttggtgt tcagcaatca aagaacctgt gacttcgctc aaatcgccat ccatgatgaa
     1021 atccaatttg tacaaggtta aattgatgcg atggtcggtg acgcggcctt gcggatagtt
     1081 gtaagtgcgg atgcgctctg agcggtcgcc actgccgacc aagctcttgc gctcagcggc
     1141 ctctttttca tgtgcttcgc ggcgttgcaa atcattcaaa cgcgccgcca ataccttcat
     1201 cgcttgcgct ttgttgctgt gttgcgaacg gccatcttga cactccacca ccaagccagt
     1261 tgggaagtgg gtgatgcgga tggcagagtc ggttttgttg atgtgctgac cgcccgcgcc
     1321 cgaggcgcgg aaggtgtcga tgcggatgtc ggcagcgttg atggcaatgt cttccaactc
     1381 atcggcttca gccatcaccg ccacggtaca ggccgatgtg tgaacacggc cttggctttc
     1441 ggtggcaggc acgcgttgca cgcggtgggc gcccgattca aatttcaatt ggctgtacac
     1501 gcctgtgccc atgatgcgca caatcgcttc tttgtagccg cccaaatcgg attcgctctc
     1561 agacaccatt tcggttttcc agccttggcg ctcggcatag cgcacataca tgcgcagcaa
     1621 atcgcctgca aacaaagccg cttcgtcgcc gcccgtgccc gcgcgcacct cgatgaacac
     1681 gtttttatcg tcgtcttggt ctttgggtaa cagcagtttt tgcaagtcca attccaaggt
     1741 ttcgattttg tcattggccg cattgatttc ttcggcggca aattctttca tgtcagggtc
     1801 ggacaacatc tcttcggcat cgattttgtc ttgctgcgct tggttaaacg cttggtaggt
     1861 ttccaccaaa ggcgtgagct cggcatattc tttgttcagt ttgcggtatt cgtccatgtc
     1921 gtcggtggcg ttttcgctcg ccaacaaatg ggtcacttct tccaagcgat cggccaattg
     1981 gtgtaatttt tcgagtaatg aggctttcat gatgactttc gtgagttaag cggcctgtaa
     2041 acgcaaacaa gcaagatgcg ctttatagca catcttgctt gtggtttaca ggccgctaca
     2101 agtgttctgg caagcggtaa atgctcgata ccgcgtcgct taattctggg cgttggttga
     2161 tttggtgcaa ggccacggta ggcgcgtgca acaatttatt ggtgagttgt aacgatagtt
     2221 tttccaacac ttcttccgcc gacacgcctt tgtgcaattg tttcaaagcg gtggccaaag
     2281 cctgttggcg ctgggtttcg ccttcgttgc gcaaggcttt gatcatgggc acgatttggc
     2341 gctcgcgttg ccaagccaaa aattcgtgta cacgggtgtc aatgatggct tcggcttcgg
     2401 cagcggcttg ttggcgcgct tctttgccca tttccaccac tttcaccatg tcgtctacgg
     2461 tgtacaaata cgcgtcttcg attttgccca cttcaggctc gatgtcgcgt ggcacggcca
     2521 agtccaataa aaacatcggg tggcggcggc gctgtttcaa agcgctttct atcatgcctt
     2581 tgccgacgat gggtaagggg ctggcggtag acgacacaat cacatcgtat tcgtgaatga
     2641 tgccaggcaa atcattgagc aacaccgcat cggtgcggcc gcccatttgg gcgcacaatt
     2701 cgcgtgcgcg ggcgagggta cggttggcca cggtgatttt cttcgggctt ttggccgaaa
     2761 aatacgttgc caccaattca atcatttcac ctgcaccaat gaacaacaca ttcaaatcgg
     2821 cgattttcgg gaagatttgt tcggccattt tcaccgaagc ggctgccata gacaccgagc
     2881 tgttgcccac ggcggtgttg ctgcgcactt ctttggccac ggaaaaggtt ttttggaata
     2941 aggtattgag ggtggtgtct atgctggact gctcttgcgc cacgcgcacc gcgtctttga
     3001 gctgacccaa aatttgcggc tcacccaata ccatcgaatc caaaccacaa gacacgcgaa
     3061 acgcatggcg tatggtttcg tcgctgtcta aagtgtataa atacgggcgg atgtcgtctt
     3121 caggcaaacg gtggaaatcg gccaaccagc caacgatgtc gctggattgg cccacacaat
     3181 acaattcggt acggttacac gtcgacaaaa tcaccgcttc acgcgcggcg tctttttgta
     3241 cgatttgttt taccgcctca ggcaacacct gtgccgcaaa cgccaagcgc tcgcgtatac
     3301 tgataggggc agtctgatgg tttaaaccga ttgcggtcag ttgcatgttg aaaacaccct
     3361 ccaaaaatag tgcctgcatt ttaccgtatt tcaccacaaa acgacacacc atcgacttgg
     3421 ttaatcactt aattaaattg aatttatcac aaatcaaatt tgcaactatg ggagccgaaa
     3481 tggatttaca cgaagtcaga caagattatg ccaaacaaag cttggaattg tccgattgca
     3541 ccgatttgcc gctgccacaa ttggaaaaat ggctacaggc tgctattcat gcacaagtgc
     3601 atgaacccac tgccatgaac gtggccactg tggatgaaaa cggacgccca aatgcgcgca
     3661 tcgtgttgtt aaaagagctg aacgaacaag gcttggtgtt tttcaccaat tacttaagcc
     3721 gaaaaggccg cgctttgacc gagaagccgt ttgccgcggt gacttttttc tggcctgaat
     3781 tggagcgtca agtgcgtgtt gagggccgcg tggtgaaatt ggatgaggcc gcttcagacg
     3841 agtattttgc ttcgcgtcct tacgccagcc gcatcggtgc ttgggcgagt gagcaaagcc
     3901 aagtcattcc tgacaaagcc catttgatga aacgcgccgc cagcataggc atcaagcatc
     3961 ctttatctgt gccgcgccca ccacattggg gtggttttgt ggtgataccc gaccgcattg
     4021 agttttggca aggtcggcct agccgtttgc acgatcgcgt gcaataccgc ttgcaagcga
     4081 ctggtgagtg ggtcaaagag cgtttggcgc cttagtttaa gctgcttaca ggccgcttga
     4141 ggtgtcaggg tttgctacgt tgtgtggcat gaagtaggaa gtgtggcaaa acccatggtg
     4201 atggccagtg tcactgttac agtgacaggt gtctgttttg aaggagtacg caatgaaaca
     4261 acatttattg ggcgtggcgt tgttggccgt cagtgcttcg gcatggtcgt tgtcgtgcac
     4321 gcctaagcct gcgtttgaag tgcgacacac tgctaatgtg tacaccaatt cggtgttttg
     4381 gctacagtcc tcagtgtcag aatcagaagc acccatttca gtggcggatt tacagttgca
     4441 gctagatggc aaggcagtgg cagcattgca ggccaatccg caaaaaatag cagagtatac
     4501 tggctttcag gtacagcctc agttgcaagc tggtggggct taccaagtgg tgttgaaaga
     4561 caaaaagcgt gacaactacg catttttaag cgatttggcc aaccgcttta ccgttgaaac
     4621 aaaaatcgtt accagtcctg atttggcatg gcgcgagtca cccaaactca tcaaagtgag
     4681 gcatcacagc ggcacatggg gcgacagtgg caatgtattg tggtcgctga aaaccacgcg
     4741 gtcattggaa gactatttgg tggtaatcca gttggcacca gagcgcaata tggaaaaggt
     4801 gcaaaccttc atcgtgaccc ctgtttcttt ggagggccaa gaagtggtgg gcgtgggttt
     4861 tgggccttgc caatatggcc ctgagactta tgtgttcagt aaaaacagca cggtgtgggc
     4921 cacgtttgat ttgctcagtc acgatggcaa gcggatacct tggcaaggtg agccacaaaa
     4981 aaccaagctc actccccgtt aataccttac aggccgcttt aagcggcctg taaatctatt
     5041 ggagcaatga attgagattg tgtttacaaa ttgccttgat tcacacgctt g
//
LOCUS       DAOMVK010000119         5070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_4598_length_5070_cov_2.768495, whole genome shotgun sequence.
ACCESSION   DAOMVK010000119 DAOMVK010000000
VERSION     DAOMVK010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4598_length_5070_cov_2.768495"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..453
                     /locus_tag="PK856_05305"
     CDS             214..453
                     /locus_tag="PK856_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01820.1"
                     /translation="MSSNVYFNIHPHHELATVAVIIVKEMIAAKHKYGVVQGCERSTS
                     KQFHSVLPASSKEPNAVGLKTWLKTYQNAKPKASK"
     gene            453..842
                     /locus_tag="PK856_05310"
     CDS             453..842
                     /locus_tag="PK856_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01821.1"
                     /translation="MNKHDANNAFEQQISNEKLAHHNQLMAEEANACNAPLDQYELGH
                     ALHVGIEAQAKAAERKKYEAEQAHHKEEQRKASIRNASVSELMRSLVAKAPTEMFPDV
                     QTYRTTGELSSRLIDWISHANNDYTRQ"
     gene            814..1626
                     /locus_tag="PK856_05315"
     CDS             814..1626
                     /locus_tag="PK856_05315"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRL01822.1"
                     /translation="MQTTTIRASSLSGLFDCPAQWKAKHIDKISMPRSPRSLIGNAVH
                     EGAAAYDASRVHGTGLTINEAAGVAVDYILDKSSEVDWIDLSPKDVEAKTLQIHQRYC
                     AEISPKFDFVIVEAACNSVNIEIDGINICLTGTPDRVYQDFAGDFGGLDLKTGFAAVS
                     KDGEVETIKHIAQAGVYEIMLEQEYNIELREAFVIAGMSTAGQAPQIGLGMIPSAKAQ
                     LLGNPENDTKGLLHYAANILKSGMFYGNPRSMTCTKQYCPIFNSCKFRGKLS"
     gene            1623..2588
                     /locus_tag="PK856_05320"
     CDS             1623..2588
                     /locus_tag="PK856_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01823.1"
                     /translation="MTQQVQTSANLLAPAQKANTSVGFFDLESFELTQRMAKMFSTSS
                     LVPAQFRDTVEKYNSATRQKELVKNPEALANCAIALDMASRLNANPIMVMQNLYVIEG
                     RPSWSSQWIIACVNKSGRFSPLQFRIEDFGEQEVQYEEFAWVSGKKQSSIKKAVIRNL
                     VCVAHAKSLDTGEVVESAPVSMELAVKEGWYGKNGSKWQTMPEVMLRYRAAAFFGRVY
                     APDLLMGFQSQEEAEELAYIETAPTGEVVENTVKVTKGKMKKAEAVTEPEPIEAAKPE
                     QGPETATVQVDDQTIDTDTGEVLPPLEPEQYPENPDADDFFAGTN"
     gene            2640..3731
                     /locus_tag="PK856_05325"
     CDS             2640..3731
                     /locus_tag="PK856_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01824.1"
                     /translation="MTELATVQTGVATPLNTIPTNSLALLQQLGAIDDQDSTEQVEIL
                     LHESANPIKWSVRQIQLALLDADALASMYQQIDDEAKAVTASVKSGDKQYFATLKTLA
                     NNVRKAKSNITNVETTIKKQLDASIELPTMTKKAIIANVKGITASIDETYTTVRAPLT
                     AKEEADKARETEIKMLLGTMEAMLKFHDPLNGSYICSTELIGRLSRLESLIPVHLKDE
                     SALVAKYQDVAKQLPDMIKNAEYQEKRDAEAKAAIEAQKKAEAKAEAAKELVQEAMQA
                     GANVANSLAQQHVAAAETKPEPVQEAVPVAAKPVLFSREQRQAMATAWMKAGADAGFT
                     SEEEIAKLKLFFNMINKNQVPYLAIEWQE"
     gene            3719..4678
                     /locus_tag="PK856_05330"
     CDS             3719..4678
                     /locus_tag="PK856_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01825.1"
                     /translation="MARITKAPQGAFQWVNNMQMLSASERMAKQREAKAALAAEREAK
                     RIERQHKKKFQARQKSYSGNSMILGEINMQRDTWAHSKVDERLSADEVLTVELHYQLH
                     LYNFWKHNAVWSRFHLDLLIEANHYLSTLYDEPDLKILCNNAFDELQTLQDHKTPNAR
                     KANMKYLIELVQWYTADLAITPKHIIKAAVAHADCYLSAVYARNAMNFPEFNYLGDML
                     SGDSITTISKKTKRPVAVTKDCLIAVTVWISRIYSHERQHILVFENDGLTKLRQHKEL
                     LIHWWQHLYTTAMQCERAVQESCIRYGWESPIANPLKALKWKH"
     gene            4666..4995
                     /locus_tag="PK856_05335"
     CDS             4666..4995
                     /locus_tag="PK856_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01826.1"
                     /translation="METLTQVCGQCGHEYEFTAEFFCRSRYSGLTIRCRACARNNVAS
                     ANAAKEKTVTLQTALKASQEAATKSMIKSLGDLSHVPERLRPLYQQYRTMLVLREYHA
                     TEYRVNV"
BASE COUNT         1536 a         1253 c         1207 g         1074 t
ORIGIN      
        1 ccaacgccgc gcaacgattg aatggcaaca ggccatgcag cgcattgaag ctgaaaccaa
       61 ggagtatgag gcagcgcttg aggctggaat gttggcgcgg cgggcaacgg gtgattatgg
      121 ggaggtttat gaataagccg cctgtaatcg tctgtctact cggcctaggt tgggaaatca
      181 tcaaaacgcc atacttttac tggagaaatc gccatgtcat caaacgtgta tttcaacatt
      241 catccgcacc acgaactggc aaccgtagcc gtaatcattg tgaaagagat gattgccgcc
      301 aagcataaat atggcgtggt gcaaggctgt gaacgcagca caagcaagca attccacagc
      361 gttttaccag cttctagcaa agagccaaac gcagttggcc tgaaaacatg gctcaaaact
      421 taccaaaatg ccaaaccgaa agcgagcaaa taatgaacaa acatgatgca aacaatgcct
      481 ttgagcagca aatcagcaat gaaaagctgg ctcatcataa ccaactaatg gcagaagaag
      541 ccaatgcctg taatgcgccg cttgatcagt atgagctggg ccatgccctg catgttggca
      601 ttgaagccca agccaaagcc gcagagcgca aaaaatacga agcagagcaa gcgcatcata
      661 aagaagagca acgcaaggca agcatacgca atgccagcgt gtccgaattg atgcgcagcc
      721 ttgttgctaa ggctcctact gaaatgtttc ccgatgtaca aacctatcga accactggcg
      781 aactgtcgtc acgtttaatt gattggattt ctcatgcaaa caacgactat acgcgccagt
      841 agcttatctg gcttgtttga ctgccctgcc caatggaaag ccaagcacat cgacaaaata
      901 tccatgcccc gctcaccacg cagcctgatt ggcaatgcgg tgcatgaggg tgcagccgct
      961 tacgatgcaa gccgtgtaca tggcacaggc ctgaccatca atgaggccgc aggcgtagca
     1021 gtggattaca tcttggacaa atccagtgaa gtggattgga ttgatttaag tcctaaggat
     1081 gttgaagcca aaaccctgca aattcaccaa cgatattgcg ccgaaatatc gcccaaattt
     1141 gattttgtga ttgtggaagc ggcctgtaat tcggtgaaca ttgaaattga tggcatcaat
     1201 atttgtttga ctggtacgcc cgaccgcgtt taccaagact ttgctggtga ttttggtggc
     1261 ttagacctga aaacaggctt tgccgcagtt tcaaaagatg gcgaagtcga aaccatcaag
     1321 cacattgccc aagcaggcgt gtacgaaatc atgcttgaac aagagtacaa catcgagtta
     1381 agagaggcat ttgtgattgc gggcatgagc accgcaggtc aagcgcctca aatcggctta
     1441 ggcatgatac caagtgccaa agctcagctt ttgggtaacc cagaaaacga caccaaaggc
     1501 ctgttgcact acgcagcaaa cattctcaaa agtggcatgt tttacggaaa cccacgcagc
     1561 atgacatgca ccaaacaata ctgccctatt ttcaactcct gtaaattcag aggtaaatta
     1621 tcatgacaca acaagtacaa acttcagcca atttattggc accagcacaa aaagccaaca
     1681 cgtcagttgg cttttttgat ttggagtcat ttgaactgac gcagcgcatg gctaagatgt
     1741 tctcaacatc cagccttgta cctgctcaat tccgagatac agtggaaaaa tacaactcag
     1801 ccacgcgaca aaaagaattg gtaaaaaacc ctgaagcttt ggccaattgc gctattgcct
     1861 tggatatggc atcacgtttg aacgccaacc caatcatggt gatgcaaaat ctgtatgtga
     1921 ttgagggtcg cccaagctgg tcaagtcagt ggatcattgc ttgcgtcaac aaatcaggcc
     1981 gcttttctcc cttgcaattt cgcatcgaag attttggcga acaagaagtg cagtatgaag
     2041 aatttgcatg ggtgagtggc aagaagcaat ctagcatcaa aaaagctgta atccgtaact
     2101 tggtctgtgt ggcccatgcc aaaagtttgg acactggcga ggtggtcgaa tctgctccag
     2161 tatctatgga gctggcagta aaagaaggct ggtatggcaa aaatggtagc aagtggcaaa
     2221 ccatgccaga ggtaatgctt cgataccgag ccgccgcttt cttcggccgc gtgtatgcac
     2281 ctgacctact gatgggcttc cagtcacaag aagaagccga agaattggct tatatcgaaa
     2341 ctgcgccaac aggcgaagtg gtcgaaaaca ctgtcaaagt gaccaaaggc aaaatgaaga
     2401 aagccgaagc tgtgacagag cctgagccaa tcgaagccgc caaacctgaa caagggcctg
     2461 aaacagccac cgtgcaagta gatgatcaaa ccatcgacac cgatactggc gaagtcttgc
     2521 caccacttga gccagagcaa taccccgaaa accctgatgc agatgacttc ttcgcgggta
     2581 ccaactaaac aacatcccaa gccacccaag cggtggcttt tctcatagaa agaaccatca
     2641 tgactgaatt agccactgta caaactggcg ttgccacccc acttaacacc ataccaacca
     2701 acagccttgc cctgcttcag cagcttggcg cgattgacga ccaagacagc accgagcaag
     2761 tcgaaatctt gctgcatgaa tcagccaatc ccatcaaatg gtcagtgcgc caaattcaat
     2821 tggccttgct tgatgccgat gcactggcat caatgtacca acaaattgac gatgaagcca
     2881 aagcagtgac cgcaagtgtt aaatcaggcg acaaacaata ctttgccaca ctgaaaacct
     2941 tggcaaacaa cgtccgtaaa gccaaatcca acattaccaa tgttgaaacg accatcaaaa
     3001 agcaattgga tgccagcatc gagctgccaa ccatgaccaa aaaggccatt attgccaacg
     3061 tgaaaggtat caccgcctca attgatgaaa cctacaccac agtacgcgcg ccgctgactg
     3121 ccaaagaaga agccgacaaa gcccgcgaga ctgaaatcaa aatgcttttg ggcaccatgg
     3181 aagctatgct gaaatttcat gaccctctaa acggaagcta catttgcagc acagaattga
     3241 taggccgcct gagccgctta gaatccttaa tccccgtgca tttgaaagat gaatccgcgc
     3301 ttgtggccaa ataccaggat gtggccaagc aattgcccga catgattaaa aatgccgagt
     3361 accaagaaaa gcgtgacgca gaagccaagg cggcaattga ggcgcagaaa aaggccgaag
     3421 ccaaagccga agcagcaaaa gaactggtgc aagaggccat gcaagcaggt gccaatgttg
     3481 ccaactcact ggcccagcag catgtggccg cagccgaaac caagcccgaa cctgtgcaag
     3541 aggctgtgcc agtggccgcc aaacctgtgc tgttcagccg cgaacaacgc caagccatgg
     3601 caaccgcatg gatgaaagca ggtgcagatg cgggcttcac atcagaagaa gaaattgcca
     3661 agttaaagct gttcttcaac atgatcaaca aaaaccaagt gccttatttg gcgattgaat
     3721 ggcaagaata accaaagccc cgcaaggggc ttttcaatgg gtgaacaata tgcaaatgtt
     3781 atccgcaagc gaacgcatgg ccaagcagcg cgaggcaaaa gctgcactgg cagccgaacg
     3841 cgaagccaag cgcatcgagc gccagcacaa aaagaaattt caagcgcgcc aaaagtcata
     3901 ctcaggcaac agtatgattt tgggtgaaat caatatgcaa cgtgacacct gggcgcattc
     3961 caaggttgac gagcgtttaa gcgcagacga ggtgctgaca gttgagctgc attaccaact
     4021 gcacttgtac aacttttgga agcacaacgc cgtgtggtca cgctttcact tggatttgct
     4081 gattgaagca aaccactatc tgtccacgct ttacgatgaa cctgatttga aaatcttgtg
     4141 caataacgcg tttgatgaac tgcaaacctt gcaggaccac aaaacaccca atgcacgtaa
     4201 ggccaacatg aaatacctga ttgagctggt gcagtggtac accgccgact tggctattac
     4261 accaaaacac atcatcaaag cggcagtggc tcatgctgat tgctatctga gtgctgttta
     4321 cgcgcgcaat gcgatgaact tccctgagtt taactatctg ggcgacatgc tctcaggtga
     4381 ttcaatcaca accatatcca agaaaacaaa gcggcctgtg gcggtcacaa aagactgttt
     4441 gattgccgtg actgtgtgga tttcacggat ttacagccat gaaagacagc atatattggt
     4501 gtttgaaaac gacggcctga ccaaactgcg acagcacaaa gagctgctaa ttcactggtg
     4561 gcagcatctg tatacaacag caatgcagtg tgagcgcgcg gtgcaggaat cgtgcattcg
     4621 ctacgggtgg gaatccccta ttgctaaccc tttaaaggct cttaaatgga aacactaacc
     4681 caagtatgcg gccagtgcgg ccacgagtac gaatttacag ccgaattttt ttgtagaagc
     4741 cgttattcag gcttaaccat tcgttgccga gcctgtgccc gcaataatgt tgcaagtgcc
     4801 aacgcggcta aggagaaaac agtaacgctt caaaccgcgc taaaagcctc acaagaagcg
     4861 gccaccaaat ccatgattaa aagccttggc gacctgtcgc atgtacctga gcggctgcga
     4921 ccgctttatc agcagtatcg gacgatgctt gtgctgcgtg agtaccacgc gactgagtat
     4981 cgggtgaatg tttaattttg ggtgaatgag atgaattatt acatgcaatt tatcggcatt
     5041 ggattgcttg cattgttttt aatttgggcg
//
LOCUS       DAOMVK010000120         5041 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_4639_length_5041_cov_2.901925, whole genome shotgun sequence.
ACCESSION   DAOMVK010000120 DAOMVK010000000
VERSION     DAOMVK010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 5041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5041
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4639_length_5041_cov_2.901925"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            490..3381
                     /locus_tag="PK856_05340"
     CDS             490..3381
                     /locus_tag="PK856_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HRL01827.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLVDLEIETLGKEQRKGNVYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSFFQSYEGGRARIQDVLKEGQEVIVQV
                     EKDERGNKGAALTTFVSLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMADLDVPQ
                     GMSLIARTAGIGRSTEELQWDLNYLLRLWELIDQAGEEHHDPYLLYLESSLVIRAIRD
                     YFKGDIGEVLVDTQEVFDEVSQFMSYVMPNNLHRVKRYEDSIPLFSRFQIEHQIETAF
                     SREVRLPSGGAIVIDHTEALVSVDVNSARSTKGADIEDTAFRTNREAAEEIARQLRLR
                     DLGGLVVIDFIDMENPKNQRDVEGTLRDALRLDRARVQMGKLSKFGLLELSRQRLQPS
                     LGESTHSACPRCAGTGFVRGVESSALHILRIIQEEAMKDNTGAVHAQVPVDVATFLLN
                     EKRAELFGIEERLDVNVVMIPSTQLETPNYKIERIRIDDTIDDETPSYRRIEAAPEAE
                     SVLNSAQKAKIQKPEPAVKGVKHDEPAPEVKPAAQAEEPVKSGFWAGLVNWFKGLFGG
                     TEAAPAETEVAPEATKAPTAANNHASYNKQPYKKRQNQRRQQNRRDDANDEIETIRPE
                     ADIPVNMEPRSAQQGGNNQSKRRQNRRQQNEQVATAVQAMAAPVAQESAENNGNTEGR
                     SNNRRRNQRGKGQNERGNAKANAVETAEVSAEQKPLVIHLNGEQSEPANKGNNQKRNN
                     RNQRNERSDRPVRAKDVMRRINVKQIAGRVRSTAYTVLVDEGLIVPKTQRRQERSPRA
                     PQVEPINIDAIAVHVSEVAAEVLGLPKPQAVMATSAPTVAPVIAQTVAPVETAAPVAV
                     AATQAPVVTQAPVPAVAEVAPVQSQPSVAAVSLPAITPVSLDVAAVAAGIDLGDLQWV
                     QTQTDLVEPYSYAPAEITGLRRADVPKVAATEAVAATDMVQIETKRDYL"
     assembly_gap    3479..3488
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3722..4147
                     /locus_tag="PK856_05345"
     CDS             3722..4147
                     /locus_tag="PK856_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01828.1"
                     /translation="MKYAIGLALLVLSVAAQACGCGGAGLAEETYRWSREVRLEMLWW
                     GVGVTLAVFGSYLILSLVGFSKRLFIAYCTVLALLNVVLSVWLFDSGRDALLGVYRAE
                     HAMLMQIGLALLPVLHAGYQMVRFVQKQTQKRPVNPPTA"
     gene            complement(4144..4704)
                     /locus_tag="PK856_05350"
     CDS             complement(4144..4704)
                     /locus_tag="PK856_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HRL01829.1"
                     /translation="MDFNLISQHLAQYKGERRIPPLEQWHPPFCGDMDLTIKANGEWW
                     HEGRRMTRQKLIDLFATVLWREGDAYFLKTPVEKIGIQVEDVPLFIEEVNLLQLADGA
                     YIECLTTHQDRIVLDSEHVLQMRASQGELKPYVTIRANLDARIARHAFYHIVSWCDLQ
                     EDGEHTMLSIPMGASQQHLSIDNTAF"
     gene            4825..>5041
                     /locus_tag="PK856_05355"
     CDS             4825..>5041
                     /locus_tag="PK856_05355"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HRL01830.1"
                     /translation="MDSVTQWVTGALLAAAVVPAKHRRAALWAGAALGTLPDLDVLPL
                     NLLNDDPVLKMTAHRSITHSLLVLPWVA"
BASE COUNT         1271 a         1139 c         1361 g         1260 t
ORIGIN      
        1 tgtgtggtta gtcttttaag caacccaaca aaactgcaaa caggctgtgt attttgaaag
       61 catatttaca ttgtgttttt aaaagtctaa aaatgatatt gttcgctggg ctgtcacagc
      121 gcggcgaaaa cattgatgtt aacgtattta tatataaaca acaatcaatg cactcgaatc
      181 agcctatgac atatttgcaa ccggtaaata tggcattaag taattaagtt tgacccctta
      241 aatatttgat ttttctggtc gtaagacgta ttgcgcggat gagcgagaat tcaaacttaa
      301 gtaataagaa gacaggtaaa gcatggtgag cacgggcggt cgttgatctt cgcctaccac
      361 accagcttgt agtatttagc gcactgcgca ttgatctttt aggtaaactt cttcaggtct
      421 ggtggtttgt aatgccactg tcaaatactg attgcgtgtg cctcgcgagt gcataaagcg
      481 aggtttttca tgaaaagaat gttatttaac gccacccaag cggaagagct acgggtagcg
      541 attgtcgacg gtcaaaattt agtcgattta gaaatagaaa cgctcggcaa agagcaacgc
      601 aaaggtaatg tctataaggg tatcatcacc cgcatcgagc catctctaga ggcttgtttc
      661 gttgattacg gtacagaccg ccatggtttt ttacctttca aagaagtttc ccgttcattt
      721 ttccaaagtt atgaaggcgg tcgtgcgcgc atccaagatg tgcttaaaga aggccaagaa
      781 gtcatcgtgc aagttgaaaa agacgagcgc ggtaacaaag gcgcggcttt aaccacgttt
      841 gtgagtcttg caggccgcta tttggtgttg atgccgaata acccacgcgg cggtggtgtt
      901 tcacgtcgca ttgaaggcga agagcgtcaa gaattgaaag cggccatggc cgatttggac
      961 gtgccgcaag gcatgagcct gattgcgcgt accgcaggca taggccgcag caccgaagaa
     1021 ttgcaatggg atttgaacta cttattgcgc ttatgggaat tgattgacca agcgggcgaa
     1081 gagcatcacg atccttattt gctctatttg gaaagctctt tggtgatacg cgccatccgc
     1141 gattatttca aaggcgacat cggcgaagtg ttggttgaca ctcaagaagt gtttgatgaa
     1201 gtgtcacaat tcatgtcgta tgtgatgccc aacaatttgc accgcgtgaa gcgttacgaa
     1261 gacagcattc ctttgttcag ccgtttccaa atcgaacacc aaatcgaaac cgcgttttcg
     1321 cgtgaagtgc gcttgccttc aggcggcgcc atcgtcattg accataccga ggctttggta
     1381 tcggtagacg tgaactctgc gcgctcaacc aaaggcgccg acattgaaga cacggctttc
     1441 cgcaccaacc gtgaggcggc ggaagaaatt gcccgtcaat tgcgtttgcg tgacttgggc
     1501 ggtttggtgg tgattgactt catcgacatg gaaaacccaa aaaaccaacg cgatgtggaa
     1561 ggtactttgc gcgatgcttt gcgcttagac cgtgcacgcg tgcaaatggg taaattgtcg
     1621 aaattcggtt tattggaatt gtcgcgtcag cgtttgcaac cttctttggg cgaatcgacc
     1681 cattcagctt gtccgcgttg cgccggcaca ggctttgttc gtggcgtaga gtcttctgca
     1741 ctgcacattt tgcgcatcat tcaagaagaa gcaatgaaag acaatactgg cgcggtgcat
     1801 gcgcaagtac cggtggatgt ggcgactttc ttgctcaatg aaaaacgtgc agaattgttt
     1861 ggtattgaag agcgtttgga tgtgaatgtg gtgatgattc caagcacaca gctggaaaca
     1921 cccaattaca aaatcgaacg catccgcatc gatgacacca ttgatgacga aacaccaagc
     1981 taccgccgaa ttgaagcggc acctgaagca gaatctgttt taaacagcgc ccaaaaagcc
     2041 aaaatccaaa aacctgagcc agcggtgaaa ggtgtgaagc acgatgagcc tgcacctgaa
     2101 gtgaaacctg ctgcccaagc cgaagagcct gtaaaatcag gattctgggc aggcttggtc
     2161 aattggttta aaggcttgtt tggtggtact gaagcagcgc ctgctgagac tgaagtcgca
     2221 ccagaagcca ctaaggcgcc tacagctgcc aataaccatg cttcatacaa taagcaacct
     2281 tataaaaagc gtcaaaacca acgccgccag caaaaccgtc gcgacgatgc caatgatgaa
     2341 attgaaacca tccgtcctga agccgacatt ccagtgaaca tggagccaag gtctgcgcag
     2401 caaggtggca acaatcaaag caaacgccgt caaaaccgcc gccaacaaaa cgagcaagtg
     2461 gctacagctg tacaagccat ggcagcgcca gttgctcaag agtctgcgga aaacaatggc
     2521 aataccgaag gccgctctaa caaccgccgt cgcaatcagc gcggcaaagg ccaaaatgag
     2581 cgtggcaatg ccaaagccaa tgcagtggaa acagcagaag tgtctgctga acaaaaacca
     2641 ttggtgatcc atttgaatgg tgagcagtct gagccggcca ataaaggcaa taaccaaaaa
     2701 cgcaataacc gcaaccaacg caatgagcgc tctgaccgcc ccgtgcgcgc caaagatgtg
     2761 atgcgtcgca tcaatgtgaa gcagattgcg ggacgcgtgc gttctacggc ttacacggtc
     2821 ttggttgatg agggtttgat tgtaccgaaa acccaacgcc gccaagagcg tagcccacgc
     2881 gcgcctcaag ttgagcctat caacattgat gcgattgccg tgcatgtgag cgaagtggct
     2941 gctgaagtgt tgggcttacc aaaacctcaa gcagtgatgg caacatctgc gccaacagtt
     3001 gcaccagtca tcgcgcaaac agtggctcct gttgaaacag ctgctccagt ggcagtggca
     3061 gctacccaag cacccgtagt gactcaggca cctgtgccag cagtggcgga agtggcgcct
     3121 gttcaaagcc agcctagtgt ggctgcagtg agcttgccag cgattactcc tgtgtctttg
     3181 gacgttgcgg cggtggcagc aggcattgat ttgggtgatt tacagtgggt acaaacccaa
     3241 accgatttgg tagagccgta ctcgtatgcg cctgctgaaa tcacgggctt gcgccgtgct
     3301 gatgtgccaa aagttgcagc tactgaggca gttgcggcca ctgatatggt gcaaattgaa
     3361 acaaagcgcg attatttgtg atttttgtac aaaaagtgct tgacgagatt gtaagctaat
     3421 cctataatct ctttctattc cctgatagct cagtcggtag agcgacggac tgttaatcnn
     3481 nnnnnnnngg ggagccaaat atgaaagccc agctgaaaag ctgggctttt ttatgtttgt
     3541 tatgtttgca tgagcaacat gagctgaata ggattgattg tatgttgtga atgcgtttgc
     3601 aggccgcttg tgagttagaa acaggcgacc tgttattttt gcatgggtat ttcactcaag
     3661 tttcactgat ttgaacagat gcatgtctac aatatatgca aaatggcatg gaggtgaggc
     3721 gatgaaatac gcaataggtt tagctttgtt ggttttgtct gtggccgcac aagcctgtgg
     3781 ctgcggtggt gcgggactgg ccgaagaaac ttaccgctgg tcacgcgagg tgcgtttgga
     3841 aatgctgtgg tggggcgtgg gtgtgacgct agcggtgttt gggtcgtatt tgattttgag
     3901 cttggtcggc ttttccaagc ggctcttcat tgcgtattgc acggtattgg ctttgctcaa
     3961 tgtggtgttg tcggtgtggc tatttgattc aggtcgcgat gcgctgttgg gagtgtaccg
     4021 cgccgaacac gcgatgttga tgcaaattgg cttggcgctg ctgcccgtgt tgcatgctgg
     4081 ctatcaaatg gtgcgtttcg ttcaaaaaca gacccaaaag cggcctgtaa acccaccgac
     4141 tgcttaaaat gcggtgttgt ctatgctgag gtgttgttgg ctagcgccca tcgggatgct
     4201 taacatggtg tgctcgccat cttcttgtaa gtcacaccat gacacgatgt ggtaaaacgc
     4261 atgtctggca atacgggcat cgaggttggc gcgtatggtc acatacggtt tcaattcgcc
     4321 ttgagaggcg cgcatttgca atacatgttc gctgtctagc acgatgcggt cttggtgggt
     4381 ggtgaggcat tcgatgtagg cgccgtctgc caattgcagc agattcacct cttcaatgaa
     4441 cagcggcaca tcttccactt gaatgccgat tttttcgact ggtgttttga gaaaatacgc
     4501 atcgccttcg cgccacaaca cggtggcaaa caaatcaatc agtttttgtc gcgtcatgcg
     4561 acggccttcg tgccaccatt cgccattggc tttgatggtt aaatccatgt caccacaaaa
     4621 tggtggatgc cactgctcca atggtggaat gcggcgttcg cctttgtatt gcgccaaatg
     4681 ttggctgatg agattaaaat ccatgtcgtg tctgtcgcgt gtatgggtta tgctcggtgg
     4741 tctcagtgta agtgtttttt gccagacaag ccataagcgg cctgtaaggc aagatgaatc
     4801 aaacaagaaa atggtgagcg gtgtatggat tctgtaaccc aatgggtaac gggcgctttg
     4861 ttggcggcgg cagtggtgcc tgccaagcac aggcgggcgg ctttgtgggc gggtgcggct
     4921 ttgggcactt tgcccgattt ggatgtgttg cctttgaact tgctcaatga cgaccctgtg
     4981 ttgaagatga cggcccaccg cagcatcacc cacagtctgt tggtgttgcc atgggtggct
     5041 t
//
LOCUS       DAOMVK010000121         4978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_4733_length_4978_cov_3.194394, whole genome shotgun sequence.
ACCESSION   DAOMVK010000121 DAOMVK010000000
VERSION     DAOMVK010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4978
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4733_length_4978_cov_3.194394"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..1081
                     /gene="hslO"
                     /locus_tag="PK856_05360"
     CDS             203..1081
                     /gene="hslO"
                     /locus_tag="PK856_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HRL01831.1"
                     /translation="MSNQRSRFILDQLPLRGVHVALEQVWQHIVSQKNYPAPIAKALG
                     ELLAAGALLAANLKFDGTFILQVQGTGVLKLLVAEATSEQTCRGTARYDDSVDIAEDA
                     SLQDLIGEQGQFVLTLQPSQGEMWQGIVGLQGDSIAQMLANYLAQSEQLDSDLILAAD
                     EHRAVGMMVQRLAGQEMAREDWQYVESLLQTVSHDELLQTDANTLLYRLFHEQEVRVF
                     EPETMEFACTCSQEKVGNMLLLLGGQEVGQILLEQGSVEIACDFCQQKYVFDEDDVKT
                     LFGSDISAAVRHELKQ"
     gene            1106..2122
                     /gene="galE"
                     /locus_tag="PK856_05365"
     CDS             1106..2122
                     /gene="galE"
                     /locus_tag="PK856_05365"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003785471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRL01832.1"
                     /translation="MKILTTGGMGYIGSHTVIALLEAGHEVVILDNLSNASRKVLARL
                     EQIAGRSIVFIEGDVRDRTVLQGLFAEHQFDAVIHFAGLKAVGESVALPLKYYDNNVA
                     GSLILMEEMAHAGVFQLVFSSSATVYGDPEQVPITETSPVGNTTNPYGTSKYMVECMM
                     RDVAAADERWNMAVLRYFNPVGAHTSGLIGESPNGIPNNLLPYICQVAVGKRECLSVY
                     GDDYDTQDGTGVRDYIHVADLADGHVKAVEVHQHDTGLQVYNLGTGQGSSVLEMVQAF
                     EAASGKKITFAITPRRPGDIATCYANTDLAQAKLKWTAKRDLQQMMQDSWRWQSQNPN
                     GYDD"
     gene            complement(2179..3546)
                     /gene="creD"
                     /locus_tag="PK856_05370"
     CDS             complement(2179..3546)
                     /gene="creD"
                     /locus_tag="PK856_05370"
                     /inference="COORDINATES: protein motif:HMM:NF008712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HRL01833.1"
                     /translation="MKLGIKFLAILIVCLLFSAVMFSINDLVEQRQTLATQVNQEMAE
                     QYVSEQTALAPFALIPIIQNCAPPIIPSANVATCTERTRWVSVPASEATWGNDLQVDD
                     TTLKRGIYHSMLFQNHMTYQAQYDFNLINLSENEHIAWEKAQWVWLLSDQRGLNQAPN
                     IDINGQKQAMQLVLQAHPRIEGAYYAAPIANPTDKPVKMGLQAAFSGTSQLNILPLAA
                     TQIHMQSPWSHPKFSGSNLPQSRQVQDSGFKANWNSAFMNESNQQYLQQCLSSAACKL
                     SLNNASEWQTASLSIFNPINVYTLSDRAIKYALLFVLMVFGTFGLFEVLKDLRVHPVQ
                     YGLVGAALAVFYLLLLSLSEHLGFTLAYVIAASACVTLIGCYVAAVLHSWLRASVLSA
                     LLISLYGVLYFLLHTSEWTLILGSGMVFVPLAVIMLLTRKLDWYGLEQRHVYRMPSGS
                     ESDSV"
     gene            complement(3626..>4978)
                     /gene="creC"
                     /locus_tag="PK856_05375"
     CDS             complement(3626..>4978)
                     /gene="creC"
                     /locus_tag="PK856_05375"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF008312.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HRL01834.1"
                     /translation="FQEQVKPNTRKVMEDILADNANVISALVAKDVANGQVKSKAFNE
                     RMQTVLEREISGKIWSIDKTAVQLRMYITDANGVVIYDSLGKFVGEDFSQWRDVYLTL
                     RGKYGARSSLDPTSPKASMSVMHVAAPIVYKGELIGVVSLGKPSASIQPFIDNAEKEV
                     WNLAVWYLFVGWIASGLMSWWLSHNINQVRRFALALAPTSQQPRFWLSKELNDLTKAI
                     ATMRTQLEDQAYVEQYVSTLTHELKSPLAGIRAGAELLQEPLPLEDQQHFADNIVAQA
                     DKLHSLIQRMLLMMRLEKSGNTTKQEPIDVPSLLNEVIAQRQSMSSSKQVDIALSGEL
                     NPKLQGDVFWLQQAIGNVLDNALDFCEVGSTIKVWGEKVGPDWLLYISNQGPQVDAFA
                     LEKVFDRYFSLARSSGNRGSGIGLTMVQEVMQRHNGYARLFNTKDGVCVSLRFHTVAI
                     "
BASE COUNT         1274 a         1118 c         1297 g         1289 t
ORIGIN      
        1 tcaaacgaaa aaacgatgtc ggtgggcgtg ttacaattca tgcccaataa ttggcacata
       61 gggattcctt tgaatgcttg ggtctctaga cagtatgacg cattatagca atggctgagc
      121 accataccaa acttgcctct tgaagatggg gcttacaggc cgcatttaaa acccctatga
      181 ccagacaaac agaaagcaag acatgagtaa tcaacgcagc cgtttcatct tagaccaatt
      241 gcctttgcgc ggcgtgcacg tggctttgga gcaggtgtgg caacacatcg tgagtcaaaa
      301 aaactaccca gcacccattg caaaagcctt gggagagttg ttggctgcgg gggctttgtt
      361 ggcggcaaac ttgaaatttg atggcacgtt cattttgcaa gtgcaaggca cgggagtgtt
      421 gaaactgttg gtggcggaag ccaccagtga gcaaacgtgc cgagggactg cgcgctatga
      481 cgacagtgtg gacattgctg aggatgccag tttgcaagac ttaattgggg agcaaggtca
      541 gtttgtgttg acgctgcaac caagccaagg cgaaatgtgg caaggcatcg tgggtttaca
      601 aggcgacagc attgcgcaaa tgttggccaa ttatttagct caatctgagc aattggacag
      661 cgatttgatt ttggctgccg atgagcatcg tgccgtgggc atgatggtac agcgcttggc
      721 aggtcaagag atggcacgtg aagattggca atatgtggag tctttgttgc aaaccgtcag
      781 ccacgatgag ttgctgcaaa ccgatgccaa taccttgctg taccgcttgt tccatgagca
      841 agaagttcgg gtgtttgaac cagaaaccat ggagttcgca tgcacttgtt cgcaagaaaa
      901 agtcggcaat atgctgctgt tacttggtgg gcaagaagtg ggacagatct tgttggaaca
      961 aggcagcgtg gaaattgcct gtgatttctg tcagcaaaaa tatgtgtttg acgaggatga
     1021 tgtgaaaacc ttatttggca gtgacatcag cgcagcagtg cgtcatgaat tgaagcagta
     1081 atacatacaa aataatgagg taacaatgaa aatattaacc actgggggca tgggctacat
     1141 tggttcgcat actgtcattg ctttattgga ggcgggccat gaagtcgtta ttctggataa
     1201 tctgagcaat gccagtcgca aggttttagc tcgtttagag cagattgcag gccgcagtat
     1261 cgtctttatc gaaggtgatg tgagagaccg tacggtctta caaggtttgt ttgctgaaca
     1321 ccagtttgat gctgtcattc attttgcggg attgaaagcc gtgggtgaaa gtgttgcttt
     1381 gccattgaaa tattacgata acaatgtggc gggctcgcta atattgatgg aggaaatggc
     1441 ccatgcaggc gtattccagt tggtgttcag ttcgtctgct acagtttatg gtgatccaga
     1501 acaggtaccc attacagaaa cctcgccagt tgggaatacc accaatccat atggtaccag
     1561 caaatacatg gtggaatgca tgatgaggga tgttgcggct gcggatgagc gttggaatat
     1621 ggcggtattg cgttacttta atcctgtggg ggcacatacc agtggtttaa tcggtgaaag
     1681 tccaaatggc ataccgaata atttattgcc gtatatttgc caagtggcag tgggtaaacg
     1741 ggagtgtcta tctgtatatg gcgatgatta tgatactcaa gatggcactg gtgtgcggga
     1801 ttacattcat gtggctgacc tggcagatgg gcatgtgaag gctgttgaag tgcatcaaca
     1861 tgatacaggt ttgcaagtgt ataacttggg tacagggcaa ggtagttcgg ttttagaaat
     1921 ggtccaagca tttgaggccg cttcaggtaa aaaaattact tttgccatta cccctcgtag
     1981 gcctggcgat attgccacat gttacgcgaa tactgacttg gcacaagcca aactgaaatg
     2041 gactgctaaa cgtgatttgc agcagatgat gcaagattca tggcgttggc agagccaaaa
     2101 tcccaatggc tacgatgatt gaagcggcct gtaaggccaa gaaagccgcc ttaagggcgg
     2161 cttttttaat gagatggttt acacgctatc agactcggaa cccgatggca tgcggtaaac
     2221 gtggcgctgt tccaatccgt accaatccaa ttttcgcgtc aacagcatga tgacggccaa
     2281 gggcacaaac accatgcctg agcccaaaat cagtgtccat tcgctggtat gcaataagaa
     2341 atacaacacg ccatacagac tgattaacaa ggcgcttaac acactggcgc gcaaccagct
     2401 gtgtaacacc gctgccacat aacagccaat gagggtcacg caagcactgg cggcaatgac
     2461 ataggccagt gtaaagccta aatgctcgct gagagaaagc aagagcagat agaacaccgc
     2521 caaagccgct ccgaccaagc catattgcac agggtgtaca cgcaaatctt tcaagacttc
     2581 aaacaagcca aatgtcccaa acaccatcaa cacaaacaac aaagcatatt ttatggcacg
     2641 gtcgctcaaa gtgtacacat tgatgggatt gaaaatggac aatgaggctg tttgccattc
     2701 tgaggcattg ttaagactga gtttgcacgc ggcactgctc agacattgtt gcaaatattg
     2761 ctggttgctt tcgttcatga aggcgctgtt ccagttggct ttaaaaccag aatcttgtac
     2821 ttgtcgcgat tggggcaaat tactgcctga aaatttggga tgtgaccatg gtgattgcat
     2881 gtggatttgg gtcgcagcca aaggcaagat attgagttgg ctggtgccac tgaatgcggc
     2941 ctgtaaaccc attttcactg gcttatctgt ggggtttgct atgggtgccg cataatacgc
     3001 cccttcaatc cgcgggtgag cttgcaatac caattgcatg gcctgttttt gaccattgat
     3061 gtctatgttt ggggcttgat tcaggccgcg ttgatcgctc aatagccaga cccattgtgc
     3121 tttttcccaa gctatgtgtt cgttttcgga cagattgatc agattgaagt catattgggc
     3181 ttggtaagtc atgtggtttt gaaacagcat gctgtggtag atgccgcgct ttaacgtggt
     3241 gtcatccact tgcaagtcat tgccccatgt ggcttccgaa gcaggaacgc tgacccagcg
     3301 cgtgcgctca gtacaggtag caacattggc actggggatg atgggtgggg cacaattttg
     3361 gatgatggga atcagcgcaa acggtgccaa cgcggtttgc tctgacacat attgttcggc
     3421 catttcttgg ttgacttgtg tggccaatgt ttgcctttgt tccaccaagt cattgatgct
     3481 gaacatgaca gcagaaaaca gcaaacacac aatcaagatg gcgagaaatt tgatgcccag
     3541 tttcatggcg gcactcctat cacagtgggt tagaccgtta cagaatgccg ttcttgtgtg
     3601 tggacttggt taagcaagca tgaaatcaaa tcgccactgt gtgaaacctc aatgaaacgc
     3661 acacgccgtc tttggtgttg aacaagcgag catagccatt gtggcgctgc attacttctt
     3721 gcaccatggt caaaccgatg ccgctgccgc ggttgccact gcttctggcc aaagaaaaat
     3781 agcggtcgaa cactttttcc aaagcaaacg catctacttg tgggccttgg ttgctgatgt
     3841 agagcaacca atcggggccc actttttcgc cccacacctt aatggtgctg cccacttcac
     3901 aaaagtccaa ggcattgtcg agcacattgc ctattgcctg ttgcagccaa aaaacatcgc
     3961 cctgtaattt gggattgagc tcaccactca aagcaatgtc cacttgtttg ctgctgctca
     4021 tggattggcg ctgtgcaatt acctcattaa gcaaactggg cacatcgatt ggctcttgtt
     4081 tggtggtgtt gccgcttttt tccaaacgca tcatcagcaa catacgctgt atcaacgagt
     4141 gcagtttatc ggcttgagcg acgatgttgt cagcaaagtg ttgttggtct tctagcggca
     4201 aaggctcttg cagcaattct gcacctgcac gtatgcctgc caatgggctt tttaattcat
     4261 gggtgagggt gctgacgtat tgttcgacat aggcttggtc ttccaattgc gtgcgcatgg
     4321 tggcgatggc cttggtcaaa tcattcaact ccttggataa ccaaaagcgt ggctgctgac
     4381 tggtgggtgc caaagccaag gcaaaacggc gcacttgatt gatgttgtgg ctgagccacc
     4441 acgacattaa gccactggct atccagccga cgaacaaata ccacaccgcc aaattccaca
     4501 cttctttttc ggcattgtcg ataaaaggct gtatgctcgc gctgggtttg cccaaactca
     4561 ctacaccgat gagttcgcct ttgtaaacaa tgggagccgc cacatgcatc accgacatgc
     4621 tggctttggg actggtgggg tctaggctgg aacgagcgcc gtatttgccg cgcagggtca
     4681 aatacacatc gcgccattgg gagaagtctt caccgacaaa tttgcccaat gaatcataaa
     4741 tcaccacacc attggcatcg gtgatgtaca tgcgcaattg caccgcggtt ttgtctatcg
     4801 accaaatttt gccgctgatt tcgcgttcca acaccgtttg catgcgctca ttaaacgcct
     4861 tgcttttgac ttgtccgttg gccacgtctt tggccaccag tgcagaaatc acgttggcat
     4921 tgtcggccaa aatgtcttcc atcaccttgc gggtattcgg tttcacctgt tcttgaaa
//
LOCUS       DAOMVK010000122         4957 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_4766_length_4957_cov_4.472868, whole genome shotgun sequence.
ACCESSION   DAOMVK010000122 DAOMVK010000000
VERSION     DAOMVK010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4957
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4766_length_4957_cov_4.472868"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..821)
                     /locus_tag="PK856_05380"
     CDS             complement(72..821)
                     /locus_tag="PK856_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HRL01835.1"
                     /translation="MKALVAVKRVVDYNIKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVSLKEAGKLTEVVVVSIGVKQCEETLRTALAMGADRAIHVETDGKLEPLAVAKLLK
                     AVQAQENADIVLLGKQAIDDDANQTGQMLAALLNAPQATFASKVELADGEVQVMREID
                     GGLETVAIKLPAVITTDLRLNEPRYVKLPNIMQAKKKPLAKTSPAELGVDVTPRFSVQ
                     KVAEPKGRSAGVKVETVAELVAKLKNEAKVI"
     gene            complement(921..1889)
                     /gene="waaC"
                     /locus_tag="PK856_05385"
     CDS             complement(921..1889)
                     /gene="waaC"
                     /locus_tag="PK856_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HRL01836.1"
                     /translation="MNILLLRLSSMGDLIHTLPAIEDLSKHRPDVRLDWMCEAGFTDI
                     ARLHPFVQQVIPMRWRHWRKHLLQVETRSEIMHLRNILIGRDYRFCVDSQGLLKSAVF
                     GKMAHAPILGYDKHSIREPLASVFYKQKYAVNKQADAIWRNRALFAQVFGYEFASEEV
                     IFGAKVPVGVASPVTEQDYIVALHATSQDEKLWPEIMWWALFEKLHEQTGRVIYLPWG
                     NNQERERAEKWVEKFNYVKLSPKLNLLQAARLLEGAQSVIGVDTGLLHLANALNKPTV
                     GIYLHSNPALTGVQESKISTNVGDVGSCPSVDTVFNAWQYCYAAKI"
     gene            1943..2617
                     /gene="tsaA"
                     /locus_tag="PK856_05390"
     CDS             1943..2617
                     /gene="tsaA"
                     /locus_tag="PK856_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HRL01837.1"
                     /translation="MSFNIEAIAVVEAEFKQKFGIARQPGLLPAAKIKIVLHQNFSQD
                     AVRGLSEFEYIWVHFVFHDAITEGWSELVRPPRLGGKSKKGIFATRTPHRPNHLGLSL
                     LKLEEIQLDKPITLICSGADLLDGTPVIDIKPYIPFVEAKPNALSGFVDGAPPVLDVF
                     WSEIAMQQLQDSDLNQDLVEQTVAQDPRPAYQNIPDRLYYMKISGYEVQFKIDGKQAE
                     IVFLTK"
     gene            2933..3850
                     /gene="lpxC"
                     /locus_tag="PK856_05395"
     CDS             2933..3850
                     /gene="lpxC"
                     /locus_tag="PK856_05395"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HRL01838.1"
                     /translation="MLQRTLASSVHVTGVGLHSGERVSLTMHPADVDTGIRFRRTDLE
                     GEQGEIIPLSPMLINDTRLSSTIVTETGVRVGTIEHIMSALSAMGIDNVLIELNAPEI
                     PIMDGSSLPFIYLLQDAGILDQAAEKKFIRIKKPIVVEDPNKRVAFLPYDGFKVTLTI
                     DFDHPVFNRSNQTYSIDFAGASYLEEIARARTFGFMQEVELMRSHNLGLGGNLTNAIV
                     IDDFDVLNPEGLRYPDEFVRHKILDAIGDLYIAGHPIIGAFEGLKSGHAINNALLRKM
                     FSDPESFEWITFPADKGLPAAFHAIVDES"
     gene            complement(3930..>4957)
                     /gene="ddlA"
                     /locus_tag="PK856_05400"
     CDS             complement(3930..>4957)
                     /gene="ddlA"
                     /locus_tag="PK856_05400"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HRL01839.1"
                     /translation="NIVEAIDKSRFDVVLFGVDKQGQWHVHDINHYLLNADNPSNIAL
                     NPSKTEVAQVVGKQQNQWIDAGNAAPISAVDVIFPIVHGTLGEDGSLQGMLRLANLPF
                     VGSDVLGSAVCMDKDVCKRLLRDAGLNVAPFITLNRGQRNSVSFETATAKLGLPLFVK
                     PANQGSSVGVSKVKTAEQYQQAIDLAFEFDHKVLIEQGIVGREIECAVLGNEQPEAST
                     CGEVVLNSEFYAYDTKYINEDGARVEIPAAISPEANEAIRQVAIQAYQTLGCGGMARI
                     DVFLTADNEIIINEINTLPGFTNISMYPKLWEASGLAYTPLITRLIELALERHALDNG
                     LKSSMSA"
BASE COUNT         1344 a         1102 c         1066 g         1445 t
ORIGIN      
        1 tgcaaagtgg ctggattcaa ggtgttattg ttgtgttcag cgataatcaa aactgtcatg
       61 gtgcgtctcc cttagatcac tttggcttca tttttcaatt tggcaaccaa ttcagcaacg
      121 gtttccactt tcacgccagc actgcgacct ttcggttcag ccactttttg cacgctaaaa
      181 cgaggcgtaa catccacacc caactcagca ggcgaagttt tggccaatgg ctttttcttc
      241 gcttgcataa tgttcggtaa ttttacataa cgaggctcat tcaaacgcaa gtcggtggta
      301 atcaccgcgg gcaatttaat cgccacggtt tccaaaccac cgtcaatctc acgcatcact
      361 tgcacttcgc catcagccaa ttctactttg ctggcaaaag tcgcttgcgg tgcattcaac
      421 aaagccgcca acatttggcc agtttggttg gcatcatcgt caatcgcttg tttgcccaac
      481 aacacaatgt cagcattttc ttgtgcttgc accgctttca aaagcttagc cacagccaat
      541 ggctctaatt taccatcggt ttccacatga atcgcacggt cagcacccat agccaaagct
      601 gtacgcaatg tttcttcaca ttgtttcacg cctatcgaaa ccaccacaac ttcagtcaat
      661 ttgcccgctt ctttcaagct tacggcttct tcaactgcaa tttcatcaaa tgggttcata
      721 gacattttga cattaccgat atccacatct gaaccatcgg ctttgacgcg cactttgatg
      781 ttgtaatcaa caacacgctt caccgctact aatgctttca ttctctatcc tcaatcgttc
      841 gattcattaa catgaaaaat tgcccagacc agcccccatc aaggcaaaat attggctgtt
      901 tttcacgatg aatatttcag ttatattttt gctgcatagc aatattgcca agcattaaaa
      961 acggtgtcaa cacttggaca gctgccaaca tcaccaacat tggtggaaat tttactttcc
     1021 tgaacgccag ttaaggctgg attagaatgt aaataaatcc caactgttgg tttattcaaa
     1081 gcatttgcca aatgcaataa gcctgtatcc acgccgatga cggattgtgc gccttccaat
     1141 aagcgcgctg cttgcaataa attcagcttc ggagataatt taacataatt aaatttttca
     1201 acccattttt cagcccgctc tcgctcttga ttgtttcccc aaggtaaata aattactcgg
     1261 cctgtctgct catgcaactt ctcaaaaagc gcccaccaca tgatttctgg ccacagtttt
     1321 tcatcttgac tggttgcatg aagtgcaaca atgtaatctt gctccgttac tggagaagca
     1381 accccaacag gaactttggc accaaaaatc acttcttcgc tagcaaattc atagccaaaa
     1441 acctgagcaa acaaagctcg attgcgccaa atggcatctg cttgtttgtt cacagcgtat
     1501 ttttgtttgt aaaaaacact cgccaaaggc tcacggatgc tgtgtttgtc gtagcccaaa
     1561 atgggagcat gcgccatttt gccaaatacc gcgcttttca acaaaccttg actgtctaca
     1621 caaaaacgat aatcacgtcc aatcaaaata ttgcgcaagt gcatgatttc acttctggtt
     1681 tcaacttgca acaaatgttt gcgccaatgg cgccaacgca tgggtatgac ttgctgcaca
     1741 aatgggtgta agcgagcgat atcggtaaaa ccggcttcgc acatccagtc taaacgcacg
     1801 tctggcctgt gtttggacaa atcctcaatt gcaggcaagg tgtgtatcaa gtcgcccatg
     1861 ctggataaac gcagcaataa aatgttcatt ggcgctttca ggtaacataa tatttttatt
     1921 ttactggaaa atggccatga gtatgtcttt taacattgaa gcaattgctg tggttgaagc
     1981 cgaattcaag caaaagtttg gtatagcaag gcaaccaggc ttgttgcctg ccgctaaaat
     2041 caagattgtt ttacatcaaa atttttctca agacgctgtc agaggtcttt ctgaatttga
     2101 atacatttgg gtgcattttg tgtttcatga tgcgataact gaaggttggt ctgaattggt
     2161 aaggcctcca cgtttgggtg gcaaaagcaa gaaaggtatt tttgccactc gaacaccaca
     2221 tcgacccaat catttgggct tgtctttatt aaaattagaa gaaattcaac tggataagcc
     2281 cattactttg atttgtagtg gtgcagattt gttggatggc acacctgtta tcgatatcaa
     2341 accctatatt ccatttgtcg aagccaaacc aaatgcctta agtggttttg tagatggtgc
     2401 ccctcctgta ttggacgtct tttggtctga aattgcgatg caacaattgc aagattcaga
     2461 tttaaatcaa gatttggttg agcaaacagt ggctcaagat cctagacccg cttatcaaaa
     2521 cattcctgat cgtctttatt acatgaaaat cagtggttat gaagtgcaat ttaaaattga
     2581 tggtaaacaa gccgaaattg tttttttaac taaataataa ttttttataa ataaaaagat
     2641 gaagaatatg aaggcgctta aatcgcgtgc ataagccaaa tttttcatca taaagaaagg
     2701 cttgaatgaa aatatgcctg tgcataagtt taaatgtgcg taagttgtta attgtggata
     2761 aaatttaaga aaagagtaat ctgtgcataa gtttaaattt tcaacagact taaagtgtgg
     2821 gtatgtggat aagacttaca taaattttaa ctataatagc gtcgccaaaa ttaagcccgt
     2881 ttgttgggaa actgggctaa aatcattcca tataaacagg taaatacgat ttatgttaca
     2941 gagaacttta gcgtcttcgg tgcatgtgac gggtgtgggt ctgcactctg gtgaacgggt
     3001 gagtttgacc atgcatcctg ctgatgtgga cacaggcata cgttttcgcc gtaccgattt
     3061 ggaaggcgaa caaggtgaaa tcattccgtt gtcgcccatg ttgattaacg acacccgtct
     3121 ttcatcgacc atcgtgactg aaacgggtgt gcgtgtgggc acaattgagc acattatgtc
     3181 agcactgtcg gccatgggca tagacaatgt gttgattgaa ttgaacgcgc ctgaaatacc
     3241 catcatggat ggctcaagct tgccatttat ttatttgttg caagatgctg ggattttgga
     3301 tcaggcggca gaaaagaaat ttatccgcat taaaaaaccg atcgtggtgg aagacccgaa
     3361 taaacgggtg gcttttttgc cttacgatgg ttttaaagtg actttaacca tcgattttga
     3421 tcaccctgtg ttcaatcgct ccaatcaaac ttacagcatt gattttgcag gcgcttccta
     3481 tttggaagag attgcccgcg cccgcacttt tggctttatg caagaagtgg aattgatgcg
     3541 cagccacaat ttgggcttgg gcggtaattt gaccaatgcg attgtgattg atgattttga
     3601 tgtgttgaat ccagagggct tgcgttatcc agatgaattt gtcagacaca aaattttgga
     3661 tgcgattggc gatttgtaca ttgcgggcca tccaattatt ggggcgtttg aagggctgaa
     3721 atctggccat gccatcaaca atgccttgtt gcgcaaaatg ttcagcgatc ctgagagttt
     3781 tgagtggata acttttcctg ccgacaaagg tttgcctgct gcgtttcacg ctatagtgga
     3841 tgaatcttaa aaagccatgc aaacactggt ttaaacacat aaaagcggcc tgtaaacatt
     3901 ttcaggccgc tttttgaatg cacaatgctt tacgctgaca tggagctttt taaaccgttg
     3961 tccaaggcat ggcgctctaa agccaattct atcaagcgcg tgatgagtgg ggtgtaggcc
     4021 aagcctgagg cttcccacag ttttgggtac atgctgatgt tggtgaagcc gggcaatgtg
     4081 ttgatttcat tgatgatgat ttcattgtcg gcggttaaga acacatcaat gcgagccatg
     4141 ccaccgcagc ccaatgtttg gtaggcctgt atcgccactt ggcgtatggc ttcattggct
     4201 tctggcgaga tggcagctgg gatttcaacg cgtgcaccgt cttcgttgat gtatttggtg
     4261 tcgtaagcat aaaactcgct gttcaacacc acttcgccgc aagtgctggc ctctggttgt
     4321 tcattgccca acacggcaca ttcaatttca cgaccgacta tgccttgttc aatcaaaact
     4381 ttgtggtcaa attcaaatgc caaatcaatg gcttgttggt attgctcggc ggttttgact
     4441 ttgctcacac ccactgatga accttgattg gcaggtttca caaacaaagg caaaccaagc
     4501 ttggcagtgg cggtttcaaa gctcacgctg ttgcgttggc cgcgattaag cgtgatgaaa
     4561 ggagccacat tgaggccagc atcgcgcagc aagcgtttgc acacgtcttt gtccatgcac
     4621 acagccgaac ccaagacatc agagccgaca aaaggcaaat tggccaagcg taacatgcct
     4681 tgcaaagagc catcttcgcc taatgtaccg tgcacgatgg ggaaaatcac atctacggca
     4741 ctgatgggcg ccgcattgcc tgcatctatc cattggtttt gctgtttgcc cacgacctgt
     4801 gctacttcag tttttgaagg attgagggca atgttgctgg gattgtcggc attgagcaaa
     4861 taatggttga tgtcgtgcac atgccattgg ccttgtttgt ctacaccaaa caacaccaca
     4921 tcaaaacggc ttttatcaat ggcttcaacg atgtttt
//
LOCUS       DAOMVK010000123         4911 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_4839_length_4911_cov_3.022241, whole genome shotgun sequence.
ACCESSION   DAOMVK010000123 DAOMVK010000000
VERSION     DAOMVK010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4911
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4839_length_4911_cov_3.022241"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PK856_05405"
     CDS             <1..333
                     /locus_tag="PK856_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01840.1"
                     /translation="GLVEDSARDGVTLRIPKALEAEIFRTVPSWWWRTPKSPPQVPVH
                     LLTASDSIFYQHGFPQLIQQQYRIPYSVVEGGHMFPLETPEATAATVLEIIAQQAATL
                     DDKTASLT"
     gene            488..832
                     /locus_tag="PK856_05410"
     CDS             488..832
                     /locus_tag="PK856_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HRL01841.1"
                     /translation="MSFPNCPQCQSEYTYTDGVMLLCPDCAHEWSGDDAVTSDEAAVI
                     KDAHGTVLQDGDAVTLIKDLKIKGSSAVIKVGTKVKSIRLLPDSADGHDIDCRIEGVG
                     AMKLKSEFVKKA"
     gene            complement(990..1658)
                     /locus_tag="PK856_05415"
     CDS             complement(990..1658)
                     /locus_tag="PK856_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01842.1"
                     /translation="MFTAKTLAYGAALTLMMSTSAYAASSKHFDVSRPELMPGLSVQL
                     DLPIDKNGERWKKILNNSNTTIAEFLHTSATLAAVDIEVKEVEAFFDTDTLKLSPSDP
                     KFIHSPEVIQQLKQRYSQFKQAGDDASRILTSFKKTQVSGKAALRFQVQSQYFNDSAA
                     MRVTSQADYLAFMHDNRYVYVKMADEAFYQKGAQQALPMPFPKNIPAFYDYKSSMNSI
                     KLKY"
     gene            1951..2367
                     /locus_tag="PK856_05420"
     CDS             1951..2367
                     /locus_tag="PK856_05420"
                     /inference="COORDINATES: protein motif:HMM:NF016701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HRL01843.1"
                     /translation="MQVQGQCLCGQVAFSMVLRDTHVHACHCQMCRRQSGGPSLSVEY
                     EPDSLTYFKRDSVRIYDSSAWAQRVVCSNCATFLFWQSKDLSYVCVNVFALDVDLAQF
                     DMDTEVYIDQKPELYQFANATQQLTGQQLMDMFKAD"
     gene            complement(2496..3008)
                     /locus_tag="PK856_05425"
     CDS             complement(2496..3008)
                     /locus_tag="PK856_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01844.1"
                     /translation="MMKDFFALLDKLNESDRKQVEGYSLSEIKRIEKLYDITIQGDFL
                     EFMLRMGRCSGGLFGDDPIVLYKEQRSVRSQILFQLINLDFLHDHHYEMRAQQPFCFS
                     WESETQYLFILTQSEDQLVYGYDEYEEAIYETGKTFEEYMFAVYEYYEHYFIDDDGKR
                     IVCKGELLVF"
     gene            complement(3224..4852)
                     /locus_tag="PK856_05430"
     CDS             complement(3224..4852)
                     /locus_tag="PK856_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HRL01845.1"
                     /translation="MADFDTHLSAQAANYAQLTPIDHLVRTAEVYGDHLAIVHGELRQ
                     NWRDTFTRCKQLAAALRQCGIERNHTVAVLLPNTPAMVEAHFGIPMSGGVLCTLNIRL
                     DVEAMIFCLRHGEAEILLADTEFAEEVKHIQAALPDLKVIQVNDILGPETEAFADTDY
                     ESFLKSAIDLDNWVLPENEWDAIALNYTSGTTGDPKGVVYHHRGAMMNSISNIMDWDM
                     PKFPVYLWTLPMFHCNGWCFPWTVAARAGVNVCLRRFDPRVCFRLIKEFGVTHYSAAP
                     IVHSSLANAPAELKQGIDHVVKGSIAGAAPPEAVLSAMEAIGFEITHVYGLTEVYGPA
                     TVCVQRDDWADMDISARAALNARQGVRSNLQDGLSVRDPISMEAVPHDGVTIGELMFR
                     GNIVMKGYLKNPQASDEAFAGGWFHTGDLGVVHPDGYVQIKDRSKDNIISGGENISSI
                     EVEDVIYKHPDVMNCAVVAVPHDKWGEVPFAFVELREGAVLSDTELRDYCREHLARFK
                     VPKHFAFTDIPKTSTGKVQKFALRNQAKALLAID"
BASE COUNT         1210 a         1101 c         1257 g         1343 t
ORIGIN      
        1 ggcttggtcg aagacagcgc gcgcgatggc gtgaccctgc gcatacccaa ggcgcttgag
       61 gcggagattt tccgcaccgt gccttcatgg tggtggcgca cgcccaaatc accgccacaa
      121 gtgcctgtgc atttactcac cgccagcgac agcattttct accaacatgg ctttccgcaa
      181 ttgatacaac agcaataccg cattccctac agtgtggtcg aaggtggcca catgttcccc
      241 ttggaaaccc ctgaagccac tgccgcaact gtgttagaaa tcattgcgca gcaagcggca
      301 acgcttgacg ataagacagc aagcttgact taagcccact gataagcggc ctgtaaacct
      361 tgtggcgttc ttgattgggt gaaatgcttg atgcaaggta aacaaagccg caattttaat
      421 tggtaatcgt ttcttatttg gccagtgatg ttacagtgcg cttttgtttg ttgcttgaga
      481 ggtcaaaatg tcttttccta actgtccaca atgccaatct gaatacacct ataccgatgg
      541 tgtgatgttg ctttgcccag attgtgcgca tgagtggagc ggtgacgatg cggtgacgtc
      601 agatgaagcc gccgtcatca aagacgcgca cggtacggta ttgcaagacg gcgatgcggt
      661 cacactgatt aaggatttga aaatcaaagg ttcgtctgcg gtgattaaag tgggcaccaa
      721 agtcaaatca atacgcttat tgcctgactc tgccgatggt cacgacatcg attgccgcat
      781 tgaaggcgtg ggggcgatga aattgaaatc agaatttgtc aaaaaagcct gaggatgaca
      841 gcggcactgc ggatgaagct gggctcgatg ttctgcccag tatcatgcag cagtgatgag
      901 gtgtatagaa acgcgattat tgaattcaac aagcgccctg taaacatgtt ttaaacattt
      961 acagaccgct tgtttgtggc tgtgcggctt cagtatttga gtttgatgct gttcatgctg
     1021 gatttgtaat cgtaaaatgc aggaatgttt ttgggaaacg gcataggcaa ggcttgttgc
     1081 gccccttttt ggtaaaaggc ttcatcggcc attttgacgt aaacatagcg attgtcgtgc
     1141 ataaacgcca aataatcggc ttggctggtc acacgcatgg cggccgagtc attgaaatat
     1201 tggctttgta cctgaaagcg cagcgccgct ttgccactca cttgggtttt cttgaatgag
     1261 gtgaggatgc gactggcatc gtcgcctgct tgtttgaatt ggctgtaacg ctgtttgagc
     1321 tgttggatga cttcggggct gtggatgaat ttggggtcgc tgggcgagag cttgagcgtg
     1381 tcggtatcga aaaatgcctc cacttctttg acttcaatgt ctaccgcggc caaggtggca
     1441 gaggtgtgca aaaattcagc gatggtggtg ttgctgttat tgagtatctt tttccaccgt
     1501 tcgccgtttt tatcaatggg caaatccagt tgcacgctca agcctggcat caattcagga
     1561 cggctgacat cgaaatgttt gcttgaggcg gcgtatgcag aggtactcat cattaaggtc
     1621 aaagctgcgc cataggccaa ggttttggct gtaaacataa acaccttctc attctgtttg
     1681 aatcatgtgt gacgaggcta acacaaatca agacagcaac tgagtatgtg gtcagttttc
     1741 ggtaggctgt gtggtgtgtg gtcgacaatg ggtctgtgca gccacagggt gttgctgagt
     1801 attcagaccc agcaggacaa acatggattg gtgttgtctt gtctatacaa gttagtctga
     1861 attttactgt tggctttggt ggtgttgtac aggttgaatg ttgacgttat gattttgaat
     1921 acagagtgta ttttcaatga ggagaacagt atgcaagtgc agggacagtg tttgtgtgga
     1981 caggtcgcgt tttcaatggt tttgcgagac acgcatgtgc atgcgtgtca ttgccaaatg
     2041 tgtcgccgtc aaagcggtgg gccatcgctg tcagtagaat atgagcctga cagcttgacg
     2101 tatttcaagc gtgacagcgt gcggatttac gattcttctg catgggcgca gcgggtggtt
     2161 tgctcgaatt gcgcgacgtt cttgttttgg cagagcaaag acttgtctta tgtttgcgtc
     2221 aacgtgtttg ccttggatgt ggatttggca cagtttgaca tggataccga agtgtacata
     2281 gaccaaaaac ctgagttata ccaatttgcc aacgccaccc agcaattaac ggggcagcag
     2341 cttatggaca tgttcaaagc tgattgaggt cgcaagtttc ttttgagata gtaagtttct
     2401 tgtggcaata ccagcaaaat aagcggtctg taagggattc ttttgctgga tatattgtac
     2461 agagagatac acaaaattca gcgcaggcac attatttaaa aaaccagcaa ctctcccttg
     2521 caaacaattc ttttaccatc atcgtcaata aaataatgtt catagtattc atatacagca
     2581 aacatatatt cttcaaaggt ttttcccgtt tcatagattg cttcctcata ttcatcgtat
     2641 ccataaacca attgatcttc ggattgagtc aatatgaata agtattgtgt ctctgattcc
     2701 catgaaaagc aaaatggctg ctgcgcgcgc atctcataat gatggtcatg aagaaaatct
     2761 aagttgatga gttgaaagag aatttgtgat cttactgacc tttgttcctt atataaaaca
     2821 attgggtcat ctccaaataa tccgccactg catcgaccca ttctcagcat aaactcaaga
     2881 aagtcaccct gtatcgtaat gtcgtagagt ttttctattc tcttgatttc acttaaggaa
     2941 tagccttcga cttgttttct atctgattca tttaatttgt ctagtaaagc aaaaaaatct
     3001 ttcatcatgg atttaccttt gtgcttcatt attttgtcta aaataaggct gcctatttca
     3061 ttgtgcgaaa tggggatgcg cgctagatat attgagggtt tgtatttacc aataccaccg
     3121 agcaaataat aggtgagcat tgatggcgat gtttaagcca gaagaatgat gcagatgggc
     3181 aatacaagcg gcctgtaaag agtttacagg ccgcttgtgg aacttagtct atggccaaca
     3241 aagctttggc ttggttgcgc aaggcgaatt tttgtacctt gcctgtcgag gttttgggga
     3301 tgtcggtaaa ggcgaagtgt ttgggcactt tgaagcgggc caaatgctcg cgacaataat
     3361 cgcgcaattc ggtgtcgctc aaaaccgcac cttcgcgcaa ttccacaaag gcaaacggca
     3421 cttcgcccca tttgtcgtgt ggcacggcca ccacggcgca attcatcaca tcggggtgtt
     3481 tgtaaatcac gtcttctact tcaatagagg aaatgttttc cccacctgaa ataatgttgt
     3541 ctttgctgcg gtctttgatt tgcacatagc catcgggatg gaccacgccc aaatcgcctg
     3601 tgtggaacca accgcctgca aacgcttcat cggaggcttg cggatttttc aaatagcctt
     3661 tcatcacaat gttgccgcgg aacatcaatt cacctatggt gacgccatcg tgtggtacgg
     3721 cttccatgct gatggggtcg cgcacgctca agccgtcttg caaattcgag cgcacccctt
     3781 gacgggcatt cagggcggcg cgggcggaga tgtccatgtc tgcccaatca tcgcgttgca
     3841 cgcagacggt ggcagggccg taaacttcgg tgaggccata cacgtgggtg atttcaaaac
     3901 caatggcttc catggccgac aacaccgctt cgggcggcgc tgcgcctgca atcgagcctt
     3961 tcaccacatg gtctatgcct tgtttgagtt cagcaggcgc attggccaag ctggaatgca
     4021 caatcggcgc ggcgctgtaa tgggtgacgc caaactcttt gatgaggcgg aaacacacgc
     4081 gtgggtcgaa gcggcgcaaa cacacgttca cacctgcacg ggcagccacc gtccatggga
     4141 aacaccagcc attgcaatga aacatcggca gcgtccacaa atacacgggg aatttgggca
     4201 tgtcccaatc catgatgttg gaaatcgagt tcatcatcgc gccgcggtgg tggtagacca
     4261 cgcctttggg gtcgcccgtg gtgcccgaag tgtaattgag cgcaatcgca tcccattcgt
     4321 tctcgggcaa tacccagttg tccaaatcaa ttgccgactt taaaaagctt tcataatcgg
     4381 tgtcggcaaa cgcttctgtt tcagggccca gtatgtcgtt gacttgtatg actttcaaat
     4441 caggcaatgc ggcctgtatg tgtttcactt cttcggcaaa ttcggtgtca gccagcagga
     4501 tttcggcttc gccatggcgc aaacaaaaaa tcatcgcctc cacatccaag cgtatgttca
     4561 aagtgcacaa cacgccgccc gacatgggaa taccaaaatg tgcttcgacc atggcagggg
     4621 tgttgggcaa caacacggcc acggtgtgat tgcgctcgat gccgcattgg cgcagtgccg
     4681 ctgccaattg cttgcaacgg gtaaaggtat cgcgccaatt ctggcgtaac tcaccatgaa
     4741 caatggccag atgatcgccg tacacttcgg cagtgcgcac caaatggtct attggtgtga
     4801 gttgggcgta attggcggct tgagcggaca aatgggtatc aaaatctgcc atgtggtttc
     4861 cttgtgtaga agcattggtt ttgtcatttt tttgatatta ataaatattt t
//
LOCUS       DAOMVK010000124         4887 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_4879_length_4887_cov_3.032078, whole genome shotgun sequence.
ACCESSION   DAOMVK010000124 DAOMVK010000000
VERSION     DAOMVK010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4879_length_4887_cov_3.032078"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PK856_05435"
     CDS             <1..295
                     /locus_tag="PK856_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HRL01846.1"
                     /translation="EATVGIDYMRLKQSFSTRNTNVDGREIRMQLLEGPFKSLEGIWK
                     FTPFGDIGCRIDFQLQYEFASGLISHIIAPVFGRISGTLVDSFIKEADKRHGK"
     gene            285..575
                     /locus_tag="PK856_05440"
     CDS             285..575
                     /locus_tag="PK856_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HRL01847.1"
                     /translation="MANKIAVEVVYATKEQQYVVSLEVDAGTTALAAAQLSGLYAQFP
                     HTQDYPIGIYGKAVTADTVLEAHDRVELYRPLLIDPKENRRRRAAKNKENEA"
     gene            572..1153
                     /gene="pth"
                     /locus_tag="PK856_05445"
     CDS             572..1153
                     /gene="pth"
                     /locus_tag="PK856_05445"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003791003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRL01848.1"
                     /translation="MSIQLIVGLGNIGQEYAQTRHNAGWWMLDELAATWGASFREDKK
                     YYAEVAKANTPQGEVWLMKPSTYMNKSGQAVGALANFFKIPVSDLLVLHDELDIPPGH
                     VRLKKGGGNGGHNGLKDIQAKMGSADFWRVRLGIGHPGDRNLVSGFVLNKPSRDDREA
                     IDDAIWRTRCEINDILSGDMEAAMRVLHSNNPK"
     gene            1539..2111
                     /locus_tag="PK856_05450"
     CDS             1539..2111
                     /locus_tag="PK856_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRL01849.1"
                     /translation="MSTAKDKILDAAASMFYDRGINSTGINSIIARASVAKMSLYNHF
                     PTKADLVSAYIAARHQEWLDLYAKRAAAITTPISGVLAVFDAYQDHAECEYENGFRGC
                     GLLNAAAEFGANTPERSAVNQHKQEVEAILATHVRALAVDEAQVVRLSLLLSYLLEGA
                     ITRAGLEGSSAHILNARAMAQVLLAAEVPQ"
     gene            2108..3004
                     /locus_tag="PK856_05455"
     CDS             2108..3004
                     /locus_tag="PK856_05455"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRL01850.1"
                     /translation="MISGQQNSQWGFFAVLAAAFLWGTTGTAATFAPSLNPLAIGAVA
                     MGGGGLLQALLARSSIVQYWQHIIRHKALLAVGVVSVAIYPLAFYSSMHYAGITVGTV
                     VSIGTAPLFTALLERVFDGKKLSMLWFISFVFGVVGVLFLSVGEAHGSAAAIEPALKT
                     LGIGLGLLAGLSYSLYSWIAKKLMQNGIAAKAAMGIIFGLGALILLPSLYFSGGNVLA
                     QNINIAVLAYMMLIPMFIGYLLFGYGLKHIPASRAITLTLFEPLVAALLAIILVGEQL
                     VAIGWVGMGLIFVCLALLSKAK"
     gene            complement(3098..>4887)
                     /gene="ppk1"
                     /locus_tag="PK856_05460"
     CDS             complement(3098..>4887)
                     /gene="ppk1"
                     /locus_tag="PK856_05460"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HRL01851.1"
                     /translation="TAQYQLFNQDILPALQTEGVRFYRRREWTAAQADWVEEYFLREL
                     KPILTPIGLDSSHPFPRLMNKSLNFAVELDGKDAFGRESGMAIVQAPRILPRVVQLPP
                     ELCDGQYGFVFLSSIMHAHIDTLFSGMEVKGCHQFRVTRDSELAVDEEDLKNLRTAIQ
                     GELRDRQFGEAVRLEITHTCPEHVSDFLLEQFQLSTEAMYVVNGPVNLVRVMAVTDMV
                     DRPDLKFPLFRPSIPKKLRQRERLFDNIAAQDGLLHHPYQSFQPVVDMVRQAAKDPAV
                     VAIKMTIYRTGSNSDLVKALLQAAIAGKQVTVVMELMARFDEETNVNWASQLERVGAH
                     VVYGVFGYKVHAKMALVIRRENGVLKSYAHLGTGNYHQGTSRLYTDLGIFTANEQITQ
                     DVNQLFMEITGLGQPTRLNKLFQSPFTLHKMLLNNIEREMAHVQAGGEGHVVAKMNSL
                     IEPSIIQKLYEASQAGVKIDLIVRGMCVLKPGIEGLSKNIRVRSIVGRLLEHSRVFYF
                     GNNGDSDVFISSADWMGRNFFRRIETCVPVLDPQLKQRVIRETLTLAMQDSAQAWDMQ
                     SDGTYIRDQAVGNEHETNLQTILMHEYSL"
BASE COUNT         1139 a         1160 c         1325 g         1263 t
ORIGIN      
        1 agaagccaca gtgggcattg attacatgcg tttgaaacaa agtttctcga cgcgtaacac
       61 caatgttgat gggcgcgaaa tccgcatgca attgctcgaa gggccgttca aatccttgga
      121 aggcatttgg aaatttaccc catttggcga catcggttgc cgcatcgatt tccaattgca
      181 atacgaattt gccagtggtt tgatttcgca tattatcgcc cctgtgtttg gccgtatttc
      241 agggactttg gtcgattcat tcatcaaaga ggccgacaaa cgccatggca aataaaatag
      301 ccgtggaggt ggtgtatgcc accaaagaac aacaatatgt ggtgagcttg gaagtagatg
      361 caggcacaac agccttggct gcagcgcaat taagcggcct gtacgcgcaa tttccacaca
      421 cacaagatta ccccataggc atttatggca aggcggtgac agccgatacg gtattggaag
      481 cacacgaccg tgttgagcta taccgtcctt tgctgataga cccgaaagaa aatcgccgcc
      541 gccgcgccgc gaaaaacaaa gaaaatgaag catgagcata caattgatcg ttggtctcgg
      601 taacataggc caagaatacg cacaaacccg ccacaacgct ggctggtgga tgttggacga
      661 attggccgcc acttggggcg cgtcgtttcg cgaagacaaa aagtattacg ccgaagtggc
      721 caaagccaac acgccgcaag gcgaagtgtg gctgatgaaa ccttctacct acatgaataa
      781 atcaggccaa gccgtgggcg ctttggctaa tttctttaaa attcctgtgt cagatttgtt
      841 ggtattgcac gatgaactcg acattccgcc tggccatgta cgcttgaaaa aaggcggtgg
      901 caatggcggc cacaatggtt tgaaagacat acaggccaaa atgggcagcg ccgatttctg
      961 gcgtgtgcgt ttgggcatag gccatccagg cgaccgcaat ttggtgtctg gttttgtgct
     1021 caacaagccc agtcgtgacg accgcgaagc gatagacgat gccatttggc gcacccgctg
     1081 cgaaatcaat gacattttgt caggcgacat ggaagccgca atgcgggtat tgcacagcaa
     1141 caatccaaaa taatcacgat tgcatgtgtt catgtatgta gaagcggcct gtaagcagtt
     1201 acaggccgct ttctcgtgtg tggtcaatgc gagtgcagtg ttggtttatc agcatgcggg
     1261 ttaagggttg gaactgaagt aaagcaaggc ttgaggtaat gtcgaatgcg ttttgttttg
     1321 gtgtgtttaa atcgattcaa atacaatttg atgattgatt gttatttatt tgaaaacaaa
     1381 cggttaatca gtttttgatt gcagcgtaaa tccatttaca ggccgcatgt tgggatcact
     1441 aactgtgtct gtgatgtcat gtgaattgaa atcttgcttg gcaaatttca caggcttgat
     1501 tattataggt agaccagtct gtctatttga gatgagaaat gagcaccgcc aaagacaaaa
     1561 tcctcgatgc cgccgccagc atgttttacg accgaggcat taacagcacc ggcatcaaca
     1621 gcatcattgc ccgcgccagt gtcgcgaaaa tgtcgctgta caaccatttt ccaaccaaag
     1681 ccgatttggt gagcgcttac atcgctgcca gacaccaaga atggctggat ttgtatgcta
     1741 aacgtgcagc agccattacc acgccgattt caggggtgtt ggcggtgttt gatgcctacc
     1801 aagaccatgc cgaatgtgag tatgaaaatg gctttcgtgg ctgcggtttg ctcaatgctg
     1861 cggctgagtt tggtgctaac acgcctgagc gcagtgcggt aaaccaacac aagcaagaag
     1921 tagaagccat tttggccaca catgtgcggg cattggctgt ggatgaagca caggtggtgc
     1981 gtttgtcctt gttgctgtcg tatctgcttg agggtgccat tacccgtgcg ggcttagaag
     2041 gcagcagtgc gcatatttta aacgcccgtg ccatggcgca ggtcttgctc gctgctgagg
     2101 tgccacaatg atttcaggcc agcaaaacag tcagtgggga ttttttgccg tgttggcagc
     2161 tgcctttttg tggggcacca caggcacagc cgccaccttc gccccgagct tgaacccgtt
     2221 ggcgatagga gccgtggcaa tgggcggcgg cggtttgctg caagccttgt tggcgcgcag
     2281 cagcattgtg caatattggc agcacatcat ccgccacaaa gccttattgg cggtgggcgt
     2341 ggtgtcggtg gcaatctacc cattggcctt ttattcatcc atgcattacg caggcattac
     2401 cgtgggcacg gtggtgtcta ttggcaccgc gcctttgttc acagccttgc ttgagcgggt
     2461 atttgatggc aaaaaactgt cgatgctgtg gtttatcagc tttgtgtttg gtgtggtcgg
     2521 cgtgttgttt ttgtctgtgg gtgaagcgca tggcagcgct gccgcaatcg aacccgcatt
     2581 gaaaaccttg ggcataggct tgggcttgct cgcaggcctg tcgtattcct tatactcatg
     2641 gatcgcgaaa aaactgatgc aaaatggcat cgccgccaaa gccgccatgg gcattatttt
     2701 tggcttggga gcgctgattc tattgcccag cctgtatttc agcggcggca atgtgttggc
     2761 acaaaacatc aacatcgctg tgttggccta catgatgctc atccccatgt tcatcggcta
     2821 tctgcttttt ggttacggct tgaaacacat ccccgccagc cgcgccatca ccttaaccct
     2881 gtttgaaccc ctggtcgccg cattgttggc catcatattg gtgggagaac aattggtagc
     2941 catcggttgg gtgggtatgg gtttgatttt tgtgtgtttg gcattgctgt ctaaggccaa
     3001 ataagtcata ggcgccacct gacataaata agcggcctgt aaacctttct tgcaaggttt
     3061 acaggccgct tttgtactgg attgtgattg ctttagctta caaactgtat tcgtgcatca
     3121 agatggtttg taggttggtt tcgtgctcgt tgccgactgc ttggtcgcgg atgtaagtgc
     3181 cgtcgctttg catgtcccag gcttgggcac tgtcttgcat ggccaaggtg agggtttcgc
     3241 gaatgacgcg ctgtttcaat tgcgggtcga gcacgggcac gcaagtctcg atgcggcgga
     3301 agaaattgcg gcccatccag tctgcactgg aaatgaacac atcgctgtcg ccattgttgc
     3361 cgaagtaaaa cacgcgcgag tgttcaagca ggcggcccac tattgagcgc acacgaatgt
     3421 ttttagacaa gccttctatg cctggtttga gcacacacat gccgcgcacg atgaggtcta
     3481 tcttgactcc tgcttgactg gcttcataca gtttttgaat gatggatggc tcaatcagcg
     3541 aattcatttt ggcaacgaca tggccttcgc cgcctgcttg tacatgagcc atttcacgct
     3601 cgatgttgtt gagcaacatt ttgtgcaagg tgaatgggct ttggaacaat ttgttcagac
     3661 gagtaggttg gcctaggccg gtgatttcca tgaacaattg gttcacgtct tgggtgattt
     3721 gttcgttagc agtgaaaatg cccaagtcgg tgtagagccg tgatgtgcct tggtggtaat
     3781 tgcctgtgcc caagtgggcg taggatttca acacgccatt ttctcggcgg atgaccaagg
     3841 ccattttggc atggactttg tagccaaaca cgccgtacac cacgtgagcg ccgacacgtt
     3901 ctagttggct ggcccaattg acattggttt cctcatcaaa acgcgccatc aattccatga
     3961 ccactgtgac ttgtttgccc gcaatagcgg cctgtaacaa ggctttgacc aaatcggaat
     4021 tgctgcctgt gcggtaaatc gtcattttaa ttgcgaccac agcagggtct ttggcggctt
     4081 gtcgcaccat gtctacaaca ggttggaagg attggtaagg gtggtggagc aaaccatctt
     4141 gcgcggcgat gttgtcaaac agtctttcgc gctgacgcaa cttttttggg atgctgggcc
     4201 gaaacaaagg aaactttaaa tcggggcggt ctaccatgtc ggttaccgcc atcacccgca
     4261 ccaaattcac aggaccattc accacataca tcgcctctgt gcttaattgg aattgctcta
     4321 gcagaaaatc gctgacgtgt tcagggcagg tgtgggtgat ttccaagcgt acggcttcac
     4381 caaattggcg gtcacgcaat tcgccttgaa tcgcggtgcg caggtttttc aaatcttctt
     4441 cgtctactgc caattcactg tcgcgggtga cgcgaaattg gtggcaacct ttcacttcca
     4501 tgcctgagaa caaggtgtca atgtgggcat gcatgatgga cgacaagaag acaaagccgt
     4561 actggccgtc gcacaattct ggcggcaatt gcaccacacg cggcaagatg cgcggcgctt
     4621 gcacgatggc catgcctgat tcgcgcccaa aagcgtcttt gccatctaat tccacggcaa
     4681 agtttaagga tttgttcatc aagcggggga acggatgtga tgagtccaag ccaatcggcg
     4741 tgaggatggg cttcaattcg cgcagaaaat attcttctac ccaatcggcc tgcgctgcag
     4801 tccattcacg gcggcggtaa aagcgcacgc cctcggtttg caatgcaggc aaaatgtctt
     4861 ggttgaacaa ttggtattgg gcggtga
//
LOCUS       DAOMVK010000125         4886 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_4880_length_4886_cov_3.756779, whole genome shotgun sequence.
ACCESSION   DAOMVK010000125 DAOMVK010000000
VERSION     DAOMVK010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4880_length_4886_cov_3.756779"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PK856_05465"
     CDS             <1..452
                     /locus_tag="PK856_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HRL01852.1"
                     /translation="FYGGMDPQGAPLLKELRKQGVKAKFMGADGLKTSNFPTLAGDAA
                     EGAFASTASVADDKMPGRAAFAEKYKKAFGGEVVAYSPYAYDATNVVIAAMKKAQSND
                     PAKYLPELAASQFDGITGKVAFTENGDLKDGVVTIYTIKGGKWEVAQ"
     gene            complement(549..1178)
                     /locus_tag="PK856_05470"
     CDS             complement(549..1178)
                     /locus_tag="PK856_05470"
                     /inference="COORDINATES: protein motif:HMM:NF016207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HRL01853.1"
                     /translation="MHCPKAFEENRTDVLLALIQQHPLATIISHSQQGLEVNVMPVLI
                     KQNGEKIVLRGHLAKNNAQVQALAHSETILWVFQGPNTYVSPAWYPSKQLHGKVVPTW
                     NYMLVEAQGQVKVFTDEDELLHLVTDMTNEHEQQRASPWQVNDAPAQYVQSQLRGIVG
                     IEMEVGQLRGKFKLSQNRSDEDQCGVRSGIASEKAEHAILPYLSHQQTQ"
     gene            1281..2759
                     /locus_tag="PK856_05475"
     CDS             1281..2759
                     /locus_tag="PK856_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRL01854.1"
                     /translation="MPNSVLITVLATFKKQPKHNIKSLAVGLRHAIHTEALQADDCLP
                     SSRQLATALGLARSTIVTVYEQLIAEGYLQARAGSATRVAPKLTLPMQWQTPPQTHAS
                     AAADCHTANNLHLQRLAQQELTPLPHQPFSVAVPQDQVQLDAHWHRISKRIVQSPLYA
                     PTGYGPAQGDLPLREQLVVYLRQARNVHCHAEQIIITDGAQQALYLASKVLLQAQDTA
                     IIENPVYPGLHAILQDRQVNIHAIKLDEQGMQVPTLLADNISARAVFVTPSHQYPMGM
                     PMSMARRMALLQWAKQQQAWIVEDDYDSELRYQGLPFPAMQGLSPQQVIYIGTFSKVL
                     APSLRLGYVVVPPDLIDAFRGAKSIISRSSSNLTQHTIAAYMQAGYFNNHIRRIRMVY
                     AERKAYLSTLLSQAIPQLHLLSSDQGMHVVAMLPPYTDDVAIVATARAHGIALRAIST
                     MCVGQAHLSGLMFGFGGFNHAQLQTAVSRLQPIINAALQHSA"
     gene            complement(2820..3290)
                     /locus_tag="PK856_05480"
     CDS             complement(2820..3290)
                     /locus_tag="PK856_05480"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HRL01855.1"
                     /translation="MNTATHTADAIVASATAAVGGAAGAAHSSGSSDQAQTQSAYVTA
                     QALGGEVAKDQRDIAAVMWLASLIFLMIPGMLVLLNSNASPYLKQQAREALNWTSTFV
                     LGSIACTILTLIFIGGPLQILLSVAHLVFCLMGANAARSGNAFRVPMTWRPLQD"
     gene            complement(3287..3448)
                     /locus_tag="PK856_05485"
     CDS             complement(3287..3448)
                     /locus_tag="PK856_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01856.1"
                     /translation="MSYQAAGFNPNQTAIRSVQAQATGANDAAATAPVYQPYAMPKIY
                     RPLKLSLKP"
     gene            complement(3530..4846)
                     /gene="hslU"
                     /locus_tag="PK856_05490"
     CDS             complement(3530..4846)
                     /gene="hslU"
                     /locus_tag="PK856_05490"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HRL01857.1"
                     /translation="MTPQEIVSELDKHIIGQHAAKRSVAVALRNRYRRSLVDEPLRTE
                     IVPKNILMIGPTGVGKTEIARRLARLANAPFIKIEATKFTEVGYVGRDVDSIIRDLIE
                     NAIKNVRETEIKKNRDRAQDAAENRVLDALLPPPRQVGFEGEAQEVQPESNTRQKFRK
                     MLRDGSLNDKEIEIEIAQGASAMSIMGPPGMEDFTQQLQGMFANMGQGKTKKQKLRVD
                     AAMKLLLDEEAAKLINEDDMREKAVSAVENHGIVFLDEIDKIAGSDRAGADVSRQGVQ
                     RDLLPLVEGTTVQTKYGMIKTDHILFVASGAFHLSKPSDLIPELQGRFPIRVELDNLS
                     VDDFKQILTGTDACLTKQYQALLATDDVALVFTDEGVERLAQIAFQVNEKTENIGARR
                     LHTVLEKLLEDVSFNSPRGEVIIDAAYVNERLQEVAEQEDLARYIL"
BASE COUNT         1170 a         1310 c         1142 g         1264 t
ORIGIN      
        1 ttttctacgg cggcatggac ccacaaggcg cgccattgtt gaaagaattg cgtaaacaag
       61 gcgtaaaagc caaattcatg ggtgctgatg gcttgaaaac cagcaacttc cctactttag
      121 caggcgatgc agctgaaggc gcattcgcgt caaccgcctc cgtggccgat gacaaaatgc
      181 caggtcgcgc ggcttttgct gagaaataca aaaaagcctt tggtggcgaa gtggtggctt
      241 actctccata cgcttacgat gcgaccaacg tggtgattgc agcgatgaaa aaagcccaat
      301 ccaacgaccc agccaaatac ttgcctgaat tggccgccag ccagtttgac ggcatcacgg
      361 gtaaagttgc cttcaccgaa aacggtgact tgaaagacgg cgtagtaacc atttacacca
      421 tcaaaggtgg caaatgggaa gtggctcaat aagtcactga attaatataa aactcataag
      481 cggcctgtaa atgtttacag gccgcttttt gttgctctgc cacttatttc ttacatacgc
      541 taaattgttt attgcgtttg ttggtgcgat aaataaggca atatagcgtg ttcggctttt
      601 tcagaagcga tgcctgaacg caccccgcat tggtcttcat cgctgcgatt ttggctaagc
      661 ttgaatttgc cacgcaattg gccgacttcc atttcaatgc ccacaatgcc gcgtaattgg
      721 ctttgtacat attgcgcagg tgcatcattc acttgccaag gagaggcccg ttgttgttca
      781 tgctcattgg tcatgtctgt caccaagtgt aacaactcat cctcatcggt aaacacctta
      841 acctgcccct gagcctcaac cagcatgtaa ttccaagtcg gcaccacttt gccatgtagc
      901 tgtttgctcg gataccaagc aggtgacaca tacgtattag gcccttgaaa cacccacaaa
      961 attgtttcac tgtgcgccaa agcctgtact tgtgcattgt ttttggccaa atgcccacgc
     1021 aacacaattt tctctccatt ttgtttaatc aatactggca tcacattcac ttccaaaccc
     1081 tgttgagaat ggctgatgat ggtggccaat ggatgttgct gtatcaatgc caataaaacg
     1141 tctgtgcggt tttcttcaaa tgcttttgga cagtgcatgc tggtttcctc catgtctgat
     1201 gtgccaccat tgtagccaaa attggtttat atttaacgac cagttaaacc catattcacc
     1261 agaccacttt tggatgtgcc atgcccaatt ctgtgttgat tactgttttg gccacgttta
     1321 aaaagcagcc caaacacaac atcaaatccc tagccgttgg cttgcgtcac gccattcaca
     1381 ccgaagcatt acaggccgat gattgcttgc catccagccg ccagctcgcc actgcgctgg
     1441 gattggcgcg cagcaccatc gtcacggtgt atgaacaatt gattgcagaa ggctatttac
     1501 aggctcgcgc aggttcagcc acgcgcgtcg cgcccaagct caccttgccc atgcagtggc
     1561 aaacgccgcc acaaacacat gcttcagccg ctgcagactg ccacactgca aataatctgc
     1621 acctacaacg tttggcacaa caagaactca cccctttgcc gcaccagcca ttttctgtgg
     1681 cagtgcctca agaccaagtt cagctcgatg cgcattggca tcgcatcagc aagcgcattg
     1741 tgcaatcgcc tttgtatgcg ccaacaggct atggtcctgc gcaaggtgat ttacccttgc
     1801 gtgaacaatt ggtggtttat ttgcgccaag cccgcaacgt acactgccat gctgaacaaa
     1861 tcatcatcac cgatggggcg caacaagcat tgtatttggc cagcaaagtg ctcttgcaag
     1921 cacaagacac tgccatcatt gaaaaccctg tttatcctgg actgcatgcg attttacaag
     1981 acagacaagt caacatacac gccatcaaac tggatgaaca aggcatgcaa gtgcctactt
     2041 tattggctga taacatttct gcccgagcgg tgtttgtaac gccgtcgcac caatacccca
     2101 tgggcatgcc tatgagtatg gcaaggcgca tggcattgtt gcaatgggcc aaacagcagc
     2161 aagcttggat tgtggaagac gattacgaca gcgaattgcg ttaccaaggg ctgccctttc
     2221 ctgccatgca aggcttgagc ccacaacaag ttatctacat aggcacgttc agcaaggttt
     2281 tagcgccttc gctgcgtttg ggctatgtgg tagtgccgcc tgatttaatt gatgcatttc
     2341 gtggggccaa atccatcatc agtcgcagca gctccaacct cacccaacac accatcgctg
     2401 cctatatgca agcaggctat tttaacaacc acatccgccg cattcgcatg gtgtacgctg
     2461 agcgcaaagc ctatttaagc accctattaa gccaagccat tcctcagctg catttactgt
     2521 ccagcgacca aggcatgcat gttgtggcca tgctgcctcc ctatacagac gatgtggcca
     2581 ttgtggccac ggctcgcgca cacggcattg ccctcagagc catctcaact atgtgtgtag
     2641 gacaagccca cctctcaggt ttgatgttcg gttttggtgg ctttaatcac gctcaattac
     2701 aaaccgcagt gtctcgattg caacccatca ttaacgctgc actccagcac agcgcataac
     2761 aaagcggcct gtaaatgcac aatacattta caggccactt ttattgcatt agcaccacat
     2821 cagtcttgca aaggtcgcca tgtcattggc acacggaaag cgttaccact gcgggcggca
     2881 tttgcgccca tcaagcagaa caccaaatgc gccacgctca acaggatttg caatgggcca
     2941 ccaatgaaaa tcaaggtcaa aattgtgcaa gcaatgctgc ccaatacgaa ggtagacgtc
     3001 cagttcaaag cctcacgtgc ttgctgtttc agatacggtg aagcattgct gttcagcaat
     3061 accaacatgc ccggtatcat caagaaaatc aaactggcca accacatcac cgcggcgatg
     3121 tcgcgttggt ctttggccac ctcacctccc aaggcttgcg ccgtgacata agcggattgg
     3181 gtttgcgctt ggtcgctgct gccgcttgaa tgcgccgcac ccgcagcgcc gcccacggct
     3241 gctgtggctg aagcgacaat ggcatccgca gtgtgcgtgg cagtgttcac ggtttcaggc
     3301 tgagtttgag cggcctgtaa atctttggca tagcgtatgg ttggtaaacg ggcgcagtgg
     3361 ctgctgcatc gtttgcacct gtcgcttgtg cttgcacaga gcgtatggcg gtttgattcg
     3421 ggttaaaacc cgcggcttga taagacatcg tatcccctct ttctgacaca tgatacacag
     3481 tagtttacag gacgattata aaggggtgac cgcagccacc ccattgtcat tacaaaatat
     3541 aacgtgccaa atcttcttgt tcggccactt cttgtaagcg ttcgttcaca taagccgcat
     3601 ctatgatgac ttcgccgcgt ggactgttga acgatacgtc ttccaacagt ttttccaaca
     3661 cggtgtgcaa acggcgtgcg ccaatgtttt cggttttttc attcacttgg aaggcaattt
     3721 gcgccaaacg ctccacgcct tcgtcggtaa acaccagagc cacgtcatcg gtggccaaca
     3781 aggcttggta ttgcttggtc aagcaggcat cggtgccagt gaggatttgt ttgaaatcat
     3841 cgacagacaa attgtccaat tccacgcgta tcgggaaacg gccttgcaat tcaggaatca
     3901 aatccgatgg tttgctcagg tggaacgcac ctgaagccac aaacaagatg tggtcggttt
     3961 tgatcatgcc gtatttggtt tgcaccgtgg tgccttcaac caaaggcaac aaatcgcgtt
     4021 gtacaccttg gcgtgacaca tcggcacctg cgcggtcgct gccggcaatt ttatcgattt
     4081 catccaagaa cacaatgccg tggttttcca ctgctgacac ggctttttca cgcatgtcgt
     4141 cttcattgat gagcttggcg gcttcttcat ccaacaacaa tttcatcgcc gcatccacac
     4201 gcaatttttg ctttttggtt ttgccttggc ccatattggc aaacatgcct tgcaattgct
     4261 gagtgaaatc ttccattcct ggagggccca taatcgacat ggcagacgca ccttgcgcga
     4321 tttcgatttc gatttctttg tcgttcaagc tgccatcgcg caacattttg cggaattttt
     4381 gacgcgtgtt gctttcaggc tggacttctt gggcttcgcc ctcaaaaccc acttggcgtg
     4441 gtggtggcaa caaagcgtct aacacgcggt tttcggctgc gtcttgggcg cggtcacggt
     4501 tttttttgat ttcggtttca cgcacgtttt taatggcgtt ttcaatcaaa tcgcggatga
     4561 tggaatccac atcacggcca acataaccga cttcggtaaa tttggtggct tcaattttaa
     4621 tgaacggtgc attggccaaa cgggccaaac ggcgggcaat ttcggtttta cccacgcctg
     4681 ttgggcctat catcaagatg tttttgggca cgatttctgt tcgcaatggc tcatccacca
     4741 agctgcggcg gtagcggttg cgcaaggcca cggccactga gcgcttcgcc gcatgttggc
     4801 cgatgatgtg tttgtctaac tcagacacaa tttcttgagg tgtcatcact gctgctgaca
     4861 tagtgtttcg ctttcctgat ggccgc
//
LOCUS       DAOMVK010000126         4863 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_4912_length_4863_cov_3.346922, whole genome shotgun sequence.
ACCESSION   DAOMVK010000126 DAOMVK010000000
VERSION     DAOMVK010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4912_length_4863_cov_3.346922"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(288..1034)
                     /locus_tag="PK856_05495"
     CDS             complement(288..1034)
                     /locus_tag="PK856_05495"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HRL01858.1"
                     /translation="MLNNHETLSFAYGDEVIEFEVNAKLGDNSKISIKVTPDCKVQVF
                     TPLDIEHTQLVNAVKKRSRWIYQQLRVFREQTQLITPRQYVSGESHYYLGKQYQLKVI
                     ESAEEIQGVKLLRGKLQVTLRKKETKKVKDLLAQWYRVRAKEVFAKRITSVIDQALWV
                     STTPNLRLLNMQTQWGNCSPTGMLTLNPYLVKAPTQCIDYVILHELCHLAEHNHSERF
                     YYLMSQVMPDWEKRKNYLDGMAAWIFNDTS"
     gene            complement(1036..4335)
                     /locus_tag="PK856_05500"
     CDS             complement(1036..4335)
                     /locus_tag="PK856_05500"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HRL01859.1"
                     /translation="MSETISQPHHAKFQEEYSAKIPALTLLSNLGWSYLSPNEALAAR
                     EGKTDQVVLREVLRRELSKRTFVFEGKTHSLSSKSVDNLVAQLCSPVLNEGLLVANEK
                     IYNHMLYGIAVTEFINGRKATPTIAVIDWDNIHNNDFSYTEEFLVSRANGVDQRRPDM
                     VCFVNGLPWVVIEAKRPDGQSAKAPTVQEGISQCLRNQRPDEIQTLFAYSQLLLSVNG
                     YDGRYGTCYTPAKFWAVWREEDYSDAHMLALKNQAVNPEHLHSIFSNRPDHAWAWYQD
                     WLAGGELAVTGQDRLLISLLTPERLLEMMRLFTFFDKKVGKVVARYHQVFGLKRLIDR
                     INTKKASGAREGGVIWHTTGSGKSFTMVFLSKALILNEALKKCRIVVVTDRVDLESQL
                     SNAFASSGEFSSDEDKANSMANSGEALAYKIGKGKQRIIFSLIQKFNSAVKYPECVNT
                     SEDLIVLVDEGHRSQNGENHIRMTQALPNAAFVAFTGTPLLKDEKTVSKFGKIVHAYT
                     MQRAVEDQTVTPLLYEERIPDLEVNERAIDSWFDRITEGLSDKQQADLKRKFAKKGQV
                     YSADDRIRLIAWDIANHFVKNIDEGLKGQLACDSKASAIKYKQYLDELGLFESAVVMS
                     APDTREDNTEVDETSMPEVAKWWKDHVGTQEEQAYTKTVIKRFDKDPSLKLLIVVDKL
                     LTGFDEPKNAVLYIDKPLKEHNLIQAIARVNRLHDQKKFGLLIDYRGILSELDTTISK
                     YQDLAAKTQGGYDINDIAGLYSQMSTEYKRLPRLYKSLWAIFDGVKNKADIEQLRQVL
                     VPRLEHRNGEWVDVNLKVRDDFYEALTEFASCLKVALQSASFFADTSFTDVDRQRYKE
                     AVKSFTSLRQLVKQDAGETVDYDEYSEQVKKLLDKHVVGVAVQESEGMYEVGKMGKLA
                     APEEWTEDKTRNETDIIKTRVAKTISQDLQDDPYAQEAFSKLLRLAIEAAEKEFDHPL
                     KQYLLFKEFEEKLEARRLDEIPDVFHGNRHAQAYFGILKKVLPETLNSHVDTEAQAPW
                     IKLAFDMDQQVTASVAENSISPQNIEADIRKKLLPLTFQASKSAGGGIDQAKAIVEML
                     VQVVRVGLKAV"
     gene            complement(4507..>4863)
                     /locus_tag="PK856_05505"
     CDS             complement(4507..>4863)
                     /locus_tag="PK856_05505"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HRL01860.1"
                     /translation="KTHYIKGKFEAYQRTYILSHFSKTIESLFLYQFILCFLTKEIDR
                     EKQHGAMPYIKLSALQKFKIIAPPILEQEKITQVLSTADQEIQVLQQKLDCLKQEKKA
                     LMQQLLTGKRRVKVAA"
BASE COUNT         1182 a         1197 c         1015 g         1469 t
ORIGIN      
        1 ttggagtatg aaaattctca gataaaagtg tcctgtgcgg cttgaccact acaactcttt
       61 gcaaaatata ttttgcctat tcagtcagcg tacccgttac caaattcaca atatcaagtg
      121 gatagctgta tattttcaaa atacacttca ccaatacacg ataagtatca tgaatttttt
      181 atactaaatt ttccttgttt cactttaaac taaatgaaca acattattcg agatggtgtg
      241 tctcattgaa ttgaattctc ttttgataca gtttaataaa tcatacccta actagtatca
      301 ttaaaaatcc atgctgccat accatctaaa taatttttcc gtttttccca atctggcatc
      361 acttgcgaca tcaggtagta aaatcgctcg ctgtggttgt gttctgctaa atggcaaagc
      421 tcgtgcagaa tcacataatc tatacactga gtaggtgctt taaccaaata agggtttaag
      481 gtcagcatac cagtgggtga acaattgccc cattgcgttt gcatgttcag caggcgtaag
      541 ttcggcgtag tgctgaccca caaagcttgg tctatcacgc tcgtaatgcg tttagcaaaa
      601 acctctttgg ctcgaacccg ataccactgc gccaataagt ctttgacttt ctttgtctct
      661 tttttccgca gcgtcacctg tagcttgcct cgtagcaact tcacaccttg gatttcttcg
      721 gcactttcga ttactttgag ttggtattgt ttgcccaggt aataatggct ttcaccactg
      781 acgtattggc gtggggtaat caattgggtt tgctcgcgaa acacacgcaa ctgttggtaa
      841 atccagcgac tgcgtttttt cacagcattc accaattgtg tgtgttcaat atccaaaggt
      901 gtaaacacct gcactttgca atctggcgtg acctttattg aaattttgct gttgtcaccg
      961 agtttcgcgt tgacttcaaa ttcaatcact tcgtcaccgt aagcaaaact cagtgtttca
     1021 tggttgttca acatgttaaa ccgccttcaa ccccacgcgc accacttgca ccagcatttc
     1081 cacaatggcc ttggcctgat cgatgccacc acctgccgac ttagaggctt ggaaggtcaa
     1141 gggcagcagc tttttacgga tgtcggcttc aatgttttgt gggctgatgg aattctcggc
     1201 caccgatgct gtcacctgtt ggtccatgtc aaatgccagt ttgatccaag gcgcttgtgc
     1261 ttcggtatcc acatgactat ttaaggtttc aggcaaaacc ttttttaaaa tcccaaaata
     1321 tgcttgtgca tggcggttgc cgtggaacac atcagggatt tcatccaaac ggcgtgcctc
     1381 aagtttttct tcaaactctt taaacaacag gtattgcttt aatgggtgat caaactcttt
     1441 ttctgctgct tcaatcgcca agcgtagcaa tttggaaaac gcttcttggg cataaggatc
     1501 gtcttgcaag tcttgggaga tggttttggc cacacgggtt ttgatgatgt cggtttcatt
     1561 gcgggtcttg tcttcagtcc actcttcagg tgcggctaac ttgcccatct tgcccacttc
     1621 atacatgcct tcagactcct gcaccgccac accgaccacg tgcttgtcca acagcttttt
     1681 cacctgttcg ctgtactcat cgtaatcaac cgtctcgccg gcgtcttgtt tcaccaattg
     1741 gcgcaagctg gtaaacgatt tcactgcctc tttgtaacgc tgtctatcca catctgtgaa
     1801 gctggtatcg gcaaagaaac tggccgattg caaagccact ttcaagcaac tggcaaactc
     1861 ggtcaacgcc tcataaaaat cgtctctcac cttcaaattt acatcgaccc attcaccatt
     1921 gcgatgttct aaccgtggta ccaatacctg gcgcaattgt tcaatgtctg ctttgttttt
     1981 gaccccatca aaaatcgccc ataagctctt gtacaaacgt ggcaagcgtt tgtactcggt
     2041 gctcatttgg ctgtacaagc cggcaatgtc attgatgtca taaccgcctt gagttttggc
     2101 agccaaatct tggtatttgg aaatggtggt atcaagctca gacaagatgc cacggtagtc
     2161 aatcaacagc ccaaattttt tctgatcatg caaacggttg acccgtgcaa ttgcctgtat
     2221 caagttgtgc tctttcaaag gcttatcgat gtagagcacg gcattttttg gctcatcaaa
     2281 gccggttaac aatttatcca ccacaattag cagcttgagc gacgggtctt tgtcaaaacg
     2341 cttgataacg gttttggtat aggcttgttc ttcttgggtg cccacatggt ctttccacca
     2401 ctttgccact tcgggcatgc tggtttcatc gacttcggta ttgtcttcac gcgtgtcggg
     2461 tgcactcatg accactgctg actcaaacaa acccagctca tccaagtatt gtttgtattt
     2521 gatggccgaa gccttgctgt cacaagcgag ttggcctttt aagccctcat caatgttctt
     2581 cacaaaatga ttggcaatgt cccaagcaat cagacggatg cgatcatctg ccgaatacac
     2641 ttggcctttt ttggcgaatt tgcgttttaa gtcggcttgt tgtttatcag acaagccctc
     2701 agtgatgcgg tcaaaccagc tgtctatcgc gcgttcattc acttccaaat ccggaatgcg
     2761 ctcttcatac agcagtggcg tcacggtttg atcttccact gcccgctgca tggtgtaagc
     2821 gtgcacgatt ttgccaaact tactcactgt tttttcatct ttgagcaatg gtgtgcccgt
     2881 gaacgccaca aatgccgcgt tcggcaaggc ttgggtcatg cggatgtggt tttcaccgtt
     2941 ttggctgcgg tgtccctcat ccaccaacac gatcaagtct tcactggtat tcacacactc
     3001 aggatatttc accgctgaat tgaatttttg tatcaatgaa aaaatgattc tttgcttgcc
     3061 cttgccaatt ttataagcca aggcctcgcc cgagttggcc atcgaattgg ctttgtcttc
     3121 atcgctgctg aactcaccac tggatgcaaa cgcattgctt aattggcttt ccaaatccac
     3181 cctgtcggtg accaccacaa tgcggcattt ttttaaggcc tcgtttaaga ttaaggcctt
     3241 gctcaaaaac accatggtga acgatttgcc tgagcctgtg gtgtgccaaa tcaccccccc
     3301 ctcacgcgca ccactggctt ttttggtgtt gatgcggtca atcaaacgct ttaaaccaaa
     3361 cacctgatgg taacgcgcca ccaccttacc caccttctta tcaaaaaagg tgaacaaacg
     3421 catcatttcc aacaatcgct caggtgtgag caaactgatt aacaagcggt cttggcccgt
     3481 taccgccaac tcaccgcctg cgagccaatc ttggtaccac gcccatgcgt ggtcaggacg
     3541 gttagagaag atgctgtgca ggtgctcagg attaacggct tggtttttaa gcgccagcat
     3601 gtgtgcatcg gaatagtctt cttcgcgcca tactgcccaa aatttcgccg gggtgtaaca
     3661 cgtgccataa cgtccatcat atccgttcac tgacaacagc aattggctgt aagcaaacaa
     3721 ggtttggatt tcatcgggtc gttggttgcg caaacattgc gagatgcctt cttgtaccgt
     3781 tggcgctttg gccgattgcc catcaggacg cttggcctca atcaccaccc aaggcaagcc
     3841 attcacaaaa cacaccatgt ctggtctgcg ttgatcgacg ccattggcac ggctgaccag
     3901 aaattcctcg gtataggaaa aatcattgtt atggatgtta tcccaatcaa tcaccgcaat
     3961 cgtgggcgtg gcttttctgc cattaataaa ctcggtaacg gcaatgccat acagcatgtg
     4021 gttgtagatt ttttcattgg caacgagcaa accctcgttc aacaccgggc tgcacaattg
     4081 cgccaccaag ttgtcaaccg acttagacga taaggaatgg gttttgcctt caaacacaaa
     4141 cgtgcgtttg gacaattcgc gccgcaacac ctcacgcaat accacttgat cggttttgcc
     4201 ttctcgtgcc gccaaggcct cgttcggcga cagatacgac caacccaaat tgctcaataa
     4261 ggttaaggct ggaattttgg cgctgtattc ttcttggaat ttggcatggt gtggttggct
     4321 gatggtttcc gacactgaat cactcccctg aattttattt gttgtattgg ctgtacatta
     4381 tgttgatgct gattgttcta ttgtttgaat gcgctggcta tgggtcaagt gctttacagg
     4441 ccgcttaaag cggcctgtaa gccataaacg agtgttctta cacatatctg cgtcattaag
     4501 ctgcacttat gccgccacct tgacccgtcg tttgcccgtg agcaattgct gcatcaaggc
     4561 ttttttctct tgttttaagc aatctagttt ttgttgcaag acttggattt cttggtctgc
     4621 ggttgagagt acctgtgtaa ttttctcttg ctctaaaata ggaggagcta taattttaaa
     4681 cttctgaagc gcactaagct taatataagg catagctcca tgctgttttt ccctatcaat
     4741 ttctttcgtc aaaaaacata aaataaattg atataaaaat aatgattcaa ttgttttact
     4801 aaagtgagaa agaatatacg ttctttgata agcttcaaat ttacctttaa tgtagtgcgt
     4861 ttt
//
LOCUS       DAOMVK010000127         4850 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_4933_length_4850_cov_2.954745, whole genome shotgun sequence.
ACCESSION   DAOMVK010000127 DAOMVK010000000
VERSION     DAOMVK010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4933_length_4850_cov_2.954745"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="PK856_05510"
     CDS             <1..665
                     /locus_tag="PK856_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRL01861.1"
                     /translation="QGVLLLVHGEVTHSHIDIFDREAVFIDEVLLPIMAKVPSLKIVF
                     EHITTSEAAQLVQSAGENLVATVTPQHLLHNRNDMLVGGVRPHLYCLPILKRRTHQEA
                     LLAAVSGAQAHKFFLGTDSAPHEQGTKECACGCAGVFSALTAMELYAEAFESVNALDK
                     LEAFASQNGARFYGLPENSRKIVLEKRPQAVPEYVDYVTGRLIPMRAGESLGWSFAGF
                     AD"
     gene            667..1278
                     /locus_tag="PK856_05515"
     CDS             667..1278
                     /locus_tag="PK856_05515"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRL01862.1"
                     /translation="MAVNRSRRTYLSAAIASVLLPSVAWASQREETLADNVASTMRRS
                     VNNVSPARLVFSTPQAGRQWLQAMSAQLSRFIPDAREREIILINVQYEAKRAGLDVQL
                     ILGLIEVESMFRRYAISGVGARGLMQVMPFWVRHIGTTGHNLFEIRTNLRYGCTILRY
                     YIDRERGDITRALARYNGSLGRLTYPNKIYAAMRRNWIYNGAV"
     gene            1292..2479
                     /locus_tag="PK856_05520"
     CDS             1292..2479
                     /locus_tag="PK856_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL01863.1"
                     /translation="MEKSADNQLTKGEWRISGILSGVYALRMLGLFLVLPVLSLYAAE
                     LAGSKQSMWIGWAMGAYGLTQACLQLPFGMVSDKWGRKKTIYIGMTMFVVGSFIAAAA
                     TSIEWLVFARALQGAGAISAVITALMSDLIRDSVRTRAMSMVGMSIGLTFALSLVAAP
                     LLVSVIGMSGLFVLTGVSMLLAMFAVYKYVPDPEKNTSNNRLPALKIALQPQLWRLNF
                     GIFALHAAQMALFVSLPFLFLQMGIAQEAQWKVYAPMVLFGMVLMVPVIIYGEKKRKL
                     KQTLLFAIGMMVVAQTMLATKHDVVMVMTVALVIYAIAFNVLEAILPSMVAKFAPAEQ
                     RGAAMGFYSTAQSIGLFIGGVAGGGLFSRFGFEGVFVFATVLMLIWWAIAVTGAAPKP
                     REA"
     gene            2545..>2880
                     /locus_tag="PK856_05525"
     CDS             2545..>2880
                     /locus_tag="PK856_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRL01864.1"
                     /translation="MSVNKVILVGRLGRDPEVRYLPNGEAVANFGVATSEQWKDRNGE
                     RQERTEWHNITMYRRLAEIAGQYLKKGSQVYIEGRIQSRKYTGKDGVERTAYEIIASE
                     MKMIGGRDMG"
     assembly_gap    2881..2890
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2891..3100
                     /locus_tag="PK856_05530"
     CDS             <2891..3100
                     /locus_tag="PK856_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01865.1"
                     /translation="ATADRHATPARGGRDMGGSSADFDGGNDYQAAAPSAPSAPVSSV
                     PPAAPRREVNKPAPVVDDLDDDIPF"
     gene            3475..3906
                     /gene="greA"
                     /locus_tag="PK856_05535"
     CDS             3475..3906
                     /gene="greA"
                     /locus_tag="PK856_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRL01866.1"
                     /translation="MQKVPLTVRGAQLLKDELQHLKSVERPAVIEAIAEARSHGDLSE
                     NAEYDAAKEKQGFVEGRIAELESKLSYAHIVNPAELDADGKIVFAATVTLEDCDSEEN
                     VTYQIVGDDARALIGKYEGDVAEVQAPGGVREYEIVAVQYI"
     gene            3963..4406
                     /locus_tag="PK856_05540"
     CDS             3963..4406
                     /locus_tag="PK856_05540"
                     /inference="COORDINATES: protein motif:HMM:NF025046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HRL01867.1"
                     /translation="MKTLLVILQAVWLGAHITVGYVVAPLLFHFASRGEISRTTAGNV
                     AGELFHVLMYFGLFTAVAWWLFCRSVRQPSKILNVLLLLLLVSEWVISPVIEAIKHKQ
                     SQWLYDLVGGSFGMWHGISSSVYLLITLLLLLWTGKRLQINSQRR"
     gene            complement(4462..4740)
                     /gene="yhbY"
                     /locus_tag="PK856_05545"
     CDS             complement(4462..4740)
                     /gene="yhbY"
                     /locus_tag="PK856_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HRL01868.1"
                     /translation="MSKSLSSAEKAELKQQAHTLQPVVMIGQNGLTEAVVKETDANLT
                     AHELIKVRILGDDRDERKLWSEQLCEQTGAELVQQIGKILVLYRKTID"
BASE COUNT         1101 a         1029 c         1384 g         1326 t
ORIGIN      
        1 agcaaggcgt gttgctgttg gtacacggtg aagtcacgca ctcgcacatt gatattttcg
       61 accgtgaagc ggtgtttatt gatgaggtgt tgttgccgat tatggccaaa gtgccaagtt
      121 tgaaaattgt gtttgaacac atcaccacaa gtgaagcggc gcaattggta caaagcgcgg
      181 gtgaaaacct ggtggccacg gtgacgccac aacacttatt gcacaaccgc aacgacatgt
      241 tggtcggtgg tgtgcgtccg catttgtatt gcttgcccat tttaaaacgc cgtacccacc
      301 aagaggcttt gttggcggca gtgagcggcg cgcaagccca taaattcttc ttgggcactg
      361 acagtgcgcc gcatgagcaa ggcaccaaag agtgtgcgtg tggctgcgca ggcgtgttca
      421 gcgcgttgac ggcgatggaa ttgtatgcag aagcatttga atcggtgaac gcattggaca
      481 aactggaagc cttcgcctct caaaatggcg cgcgttttta tggcttgcct gaaaacagcc
      541 gcaaaatcgt gttggaaaaa cgcccacaag ccgtgcctga gtatgtggac tatgttacag
      601 gccgcttgat accgatgcgt gcaggcgaaa gcttgggttg gtcgtttgca ggctttgctg
      661 actgagatgg cggtgaaccg tagccgccgc acttatttga gtgctgccat cgccagtgtg
      721 ttgttgccga gtgtggcttg ggcatcgcaa cgcgaggaaa ccttggccga taatgtggcc
      781 tcgaccatgc gccgttcggt caacaatgtc agccctgcgc gtttggtgtt ttccaccccg
      841 caagcagggc gacagtggct gcaggcaatg tcagcacaat tgagccgttt tattcccgat
      901 gctcgcgagc gtgaaatcat cttaattaat gtgcaatacg aagcgaagcg cgcggggttg
      961 gacgtgcaat tgattttggg cttgatagaa gtcgaatcga tgtttcgtcg ttatgccatc
     1021 agtggtgtgg gtgcgcgcgg tttgatgcag gtgatgccgt tttgggtgcg ccacatcggc
     1081 accactggcc acaatttgtt tgagatacgc actaatctgc gttatggctg caccattttg
     1141 cgctattaca tagaccgtga gcgcggcgac atcacacggg ctttggcgcg ctacaacggc
     1201 agcttgggac gcctgactta cccgaataaa atttacgcag cgatgcgccg caactggata
     1261 tacaacggag ccgtttagct tggataaaaa cttggaaaaa tctgctgaca atcaattgac
     1321 caaaggcgaa tggcgcatca gtggcatctt gtctggcgtg tacgcgctgc ggatgttggg
     1381 cttgtttttg gtcttgcctg tgttgtcttt gtatgcggca gaattggctg gttcaaaaca
     1441 atccatgtgg ataggctggg cgatgggggc ttatggcttg actcaagcct gcttgcaatt
     1501 gccgtttggc atggtgtcgg acaaatgggg tcgtaaaaag accatttaca ttggtatgac
     1561 catgtttgtg gtgggcagct ttattgctgc tgctgccact tcgattgaat ggttggtgtt
     1621 tgcacgggct ttgcaaggcg cgggcgccat cagcgcggtg attacggccc tgatgtcaga
     1681 tttgatccgc gattcggtgc gcacgcgtgc catgtctatg gtgggcatga gcatcggttt
     1741 gacgtttgcc ctgagcttgg tggcagcacc tttgttggtg agcgtcatag gcatgtcggg
     1801 tttgtttgtg ttgacgggtg tgtccatgct gctcgcgatg tttgccgtgt acaaatacgt
     1861 gcctgaccct gagaaaaaca ccagtaacaa tcgtttgcct gccttgaaaa ttgctttgca
     1921 gccacagttg tggcgtttga atttcggtat tttcgccttg catgcggcgc aaatggcctt
     1981 gtttgtgtct ttgccatttt tgtttttgca aatgggcatt gcgcaagagg cgcagtggaa
     2041 agtgtatgca ccgatggtgt tgtttggcat ggtgttgatg gtgcctgtga ttatctatgg
     2101 tgagaaaaaa cgtaagttga aacaaacctt gttgtttgcc ataggcatga tggtggtggc
     2161 gcaaacgatg ttggcaacca agcacgatgt ggtgatggtg atgaccgtgg ctttggtgat
     2221 ttacgccatt gcgttcaatg tgttggaggc cattttgccg tctatggtgg ccaagttcgc
     2281 ccctgctgaa caacgtggtg ctgccatggg gttttacagt acggcacaat caatcggctt
     2341 gtttatcggt ggcgtggcag ggggcggttt gttcagtcga tttggttttg aaggtgtgtt
     2401 tgtttttgcc acagtgttga tgctgatttg gtgggcgatt gcggtcactg gggcggcgcc
     2461 gaaaccacgt gaggcgtgac gcgcgtccat cgtgtcttta tactgtcgca gtaaattaat
     2521 gaatgaagtg ttgtggagga cgttatgtca gtgaataaag tgattttggt cggtcgtttg
     2581 gggcgagacc cggaagtgcg ttacttgccc aacggtgaag ccgtggccaa tttcggcgtc
     2641 gctaccagtg aacaatggaa agaccgcaat ggtgagcgcc aagagcgcac cgaatggcac
     2701 aacatcacca tgtaccgccg tttggcagag attgctggcc agtatttgaa aaaaggcagt
     2761 caagtctata ttgaaggccg cattcaatcg cgcaaataca caggtaaaga tggtgtggaa
     2821 cgcacggctt acgaaatcat cgccagtgaa atgaaaatga taggcggccg tgacatgggt
     2881 nnnnnnnnnn gcaacggccg acagacacgc cactcccgcc cggggtggcc gtgacatggg
     2941 tggcagcagt gctgattttg atggtggcaa tgattaccaa gccgcagccc ctagcgcacc
     3001 atcggctcca gtcagctcag tgcctcccgc tgcaccacgt cgtgaagtga acaagcctgc
     3061 ccctgtggtt gacgacttgg atgacgatat cccattctaa gagggtgatg ggctgaattt
     3121 tcaggccgta tttgagatgg cctaagtgaa tgaataccgc cttaatgaat gattaggcgg
     3181 tattttgtat gccatgcgtg tgattgcaac tgtgtgtttg tggagacgga aatgtattga
     3241 tgctcggaaa tcatgtggtt tttcaggccg catgagtagg cgaaccggcg ttttgcttgg
     3301 tcattgggtc gcattgtttg atgcagctta gggagttgtg gtttaaagca atctgcttat
     3361 tgctatttct gtctggtttg ttacaatagc ttctactttt tcgaaacaag ccgccgatta
     3421 ttccggcggc ttgtattttt attgcctgaa tgacgctaaa cgtaggagag agtcatgcaa
     3481 aaagtaccat tgaccgttcg tggtgcccaa ttgttaaaag atgaattgca acacttgaaa
     3541 tcagtggagc gtccagccgt gattgaggcg attgcagaag cgcgttcgca tggcgatttg
     3601 tctgaaaatg ccgaatacga tgccgccaaa gagaaacaag gctttgttga aggccgtatt
     3661 gcagaacttg aaagcaaact ttcgtatgct cacattgtta atccagcaga gttggatgcc
     3721 gatggcaaga ttgtgtttgc cgctaccgtg actctggaag attgtgacag tgaagaaaac
     3781 gtcacttatc aaatcgtcgg tgacgatgcc cgcgcattga ttggtaaata cgaaggtgat
     3841 gtggccgaag tgcaagcacc tggtggggtg cgtgagtatg aaattgtggc ggtacaatac
     3901 atttaatcgc aaaggcttac aggccgctta atccataagc ggcctgtaat tggaaataca
     3961 ccatgaaaac actgttggtc atattacaag ccgtatggtt gggtgctcac atcaccgtgg
     4021 ggtatgtggt ggcacccttg ctgtttcatt tcgccagccg tggcgaaatc agtcggacga
     4081 cagcgggcaa tgtggcaggc gagttgtttc atgtgttgat gtattttggt ttgttcactg
     4141 cagtggcgtg gtggttgttt tgccgcagcg tgcgccaacc gtctaaaatt ttgaatgttt
     4201 tgttgttatt gttattggtg agcgagtggg tgattagccc tgtgattgaa gccatcaaac
     4261 acaaacaatc gcaatggctg tatgacttgg taggcggcag ttttggcatg tggcacggaa
     4321 tttcttccag tgtgtatttg ctgataaccc tcttattatt gctgtggaca ggtaaacgtt
     4381 tgcaaattaa cagtcagcgt cgttgagcaa tataaaagcg gcctgtaagg tttacaggcc
     4441 gcttttgcac tgatttggca atcaatctat ggttttgcga tacaaaacca agattttacc
     4501 gatttgttgt accagttcgg caccggtttg ttcgcacaat tgctcagacc acaatttgcg
     4561 ctcatcgcga tcatcgccca aaatacgcac cttaatcaat tcatgcgcgg ttaaattggc
     4621 atcggtttct ttgaccacag cctcggttaa accgttttga ccgatcatca ccacaggttg
     4681 caaggtgtgg gcttgttgtt tgagctccgc tttttcggca ctcgataaag atttagacat
     4741 ctgaagcttt cctgtaaaac gcatattgta ctggatttgt tgtacaaatt gaaaaaaact
     4801 catcaaatgt aatggattaa accaatgccc aaaagaacca aatcatcagg
//
LOCUS       DAOMVK010000128         4839 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_4954_length_4839_cov_3.021948, whole genome shotgun sequence.
ACCESSION   DAOMVK010000128 DAOMVK010000000
VERSION     DAOMVK010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4954_length_4839_cov_3.021948"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..828)
                     /locus_tag="PK856_05550"
     CDS             complement(163..828)
                     /locus_tag="PK856_05550"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRL01869.1"
                     /translation="MNLAIFDLDHTLIASDSDNEWPKYLMHKGIVDEAFVSMKNDYFY
                     QQYLRGQLNVDEFLEFQLAPLTHYSMEELAQMHQEFMRDFILPTITDAARALVTQHQQ
                     AGDDVLLISATNEFIITPIAHEFGITEVIGVTLEVGEDGRYTGGYVGVPSYQAGKITR
                     LEQWLQEQGKTRADYESIYFYSDSRNDLPLLKDVSHPVAVNPDEILLAHATEHGWPIV
                     HTH"
     gene            complement(809..1486)
                     /gene="hda"
                     /locus_tag="PK856_05555"
     CDS             complement(809..1486)
                     /gene="hda"
                     /locus_tag="PK856_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HRL01870.1"
                     /translation="MSQLIFNFDNQAYPNFAKFLGQANRELLQVLQHQHERLVYVWGA
                     EGRGKSHLLKAWTAQAISLGHAAQYWDVRTRPFDLASAQAEFIAIDGIEFLDDTEQQD
                     LFHMFNQIRDGHYGYLLMSANVPPLQLDLREDLRTRMGYALVYEVKPLSDEEKLEALK
                     SLAQSRQLAIGDELFKYLLQHWRRDMDSLVTMVQKLDDYSLMTGRRITLPLLKQLLED
                     THEFSHI"
     gene            complement(1585..2664)
                     /locus_tag="PK856_05560"
     CDS             complement(1585..2664)
                     /locus_tag="PK856_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRL01871.1"
                     /translation="MYVRPRNPYVHWIIGSLVFLVVMVLLYSLRTILTPFIAAAILAY
                     ILNPLVNKLTSAKIGRGLAAVLVTLLAIAIVVGLILFIIPMMVQQAEAAWQRVPDMVN
                     YVQHQLIPWINQTFHTHLSLNGQVVTQYVTQNSEEIRSGLSKLMPFLANKGTSLMLMA
                     ANLFLLPILLYYFLADWPRWAEGTRRLIPRRYLEQVLRIGGEIDKTLSEYLRGQLLVM
                     IIVGLIYGGGLALVGLDSGFAIGMLAGMLVVIPYLGAFIGMLLATLAALLQFGTLGGL
                     VSVWSVFLVGQMLESYVITPRLVGERIGLSALWVIFALMAFGQLLGFVGMLLALPMAA
                     ICLVLLRELTQHYFNSPYYQRHNQE"
     assembly_gap    2758..2773
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2774..>4839)
                     /gene="acnA"
                     /locus_tag="PK856_05565"
     CDS             complement(<2774..>4839)
                     /gene="acnA"
                     /locus_tag="PK856_05565"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HRL01872.1"
                     /translation="VGGIEAEAAMLGQPVSMLIPEVVGFKLTGKLREGITATDLVLTV
                     TQMLRQKGVVGKFVEFFGDGLADLPLADRATIANMAPEYGATCGFFPVDEVTLGYLRL
                     SGREEARIALVEAYCKQQGLWRNTGDEPVFTNVLELDMGMVEASLAGPKRPQDKVVLS
                     QVPQAFKQFVDSSAPVSGKDLARLIGEGGAGAAVGTEESLEEEPSIACSVDGQDLQLK
                     NGAVVIAAITSCTNTSNPSVMMAAGLVAKKALEKGLQRKPWVKSSLAPGSKVVTDYLQ
                     KAGLTDALNQLGFNLVGYGCTTCIGNSGPLPKEIEEAINQGNLTVASVLSGNRNFEGR
                     VHPLVKTNWLASPPLVVAYALAGSVKIDLDKEPLGQNAQGEDIYLKDVWPSSAEIAEA
                     VAKVDTAMFRKEYAAVFDGDATWQAIAVKESQTYDWNLDSTYIQHPPFFEHIAEPTRS
                     ITDIAGARVLAVLGDSVTTDHISPAGNIKADSPAGRYLQEKGVAPIDFNSYGSRRGNH
                     EVMMRGTFANIRIKNEMLDGVEGGFSLHVPSGKQEAIYDVAMRYQAEKTPLVIFAGKE
                     YGTGSSRDWAAKGTLLLGVKAVIAESFERIHRSNLVGMGVLPLQFLNGESRSSLGLTG
                     REQVSITGLSDDIKPQQTLTLHITREDGSQTQTEVLCRIDTLNEVEYFKAGGILHYVL
                     RQLMAA"
BASE COUNT         1264 a         1302 c         1079 g         1178 t
ORIGIN      
        1 gtaactgctc atggtttcct ttcaggccgc agcttaagcg cggtgctggt gtgtgactac
       61 tgcttagcaa tgactggcgg gccagtgtga ggcattgctg actgatgaca agcggcctgt
      121 aagcagtata gttatccaca ccacttacag gccgcttgta gcttaatgcg tgtgcacaat
      181 cggccaaccg tgctcagtgg catgtgccaa caaaatctca tcgggattaa ctgcgacggg
      241 atggctcacg tctttgagca aaggcaaatc gttgcgagaa tcgctgtaaa aataaatgct
      301 ctcgtaatca gcacgggttt tgccttgctc ttgcaaccac tgctccaaac gggtgatttt
      361 gcccgcttgg taactgggca cacccacata tccacctgta tagcgaccat cctcgcccac
      421 ttccaacgtc acgcctatca cttcggtgat accaaactca tgggcaatcg gtgtgatgat
      481 gaactcattg gttgctgaaa tcagcaacac atcgtcaccc gcttgttggt gttgtgtcac
      541 caaggcacga gcggcatcgg taattgtggg caatatgaaa tcacgcatga attcttggtg
      601 catttgcgcc aattcttcca tactgtagtg tgtgagcggc gccagctgaa attccaaaaa
      661 ttcatccaca ttcaactgcc cacgcaaata ctgctggtag aaatagtcgt ttttcatgct
      721 cacaaaggct tcatcaacta ttcccttgtg catcaggtat ttcggccatt cattgtcaga
      781 gtcgctggcg attaaggtgt ggtctaggtc aaatatggct aaattcatgg gtgtcttcaa
      841 gcagttgttt gagtaggggt aaggtgatgc ggcggcccgt catcaaagag taatcgtcta
      901 atttttgcac catggtcacc aaggaatcca tgtcgcgccg ccaatgttgc aacaggtatt
      961 tgaacaactc atcgcctatc gccaattgcc gcgattgggc caagcttttc aaagcttcca
     1021 gcttttcttc atcgctcaat ggtttcactt catacaccaa agcatagccc atgcgcgtac
     1081 gcaaatcctc acgcaaatcc aattgcaatg gcggtacgtt cgcactcatc agcaaatagc
     1141 cataatggcc atcgcgaatt tggttgaaca tgtgaaacaa gtcttgttgt tcggtgtcgt
     1201 cgagaaattc gatgccatcg atggcgatga attcggcttg ggctgaggcc aagtcaaatg
     1261 gacgggtacg cacatcccaa tactgagcag catggcctag actgatggct tgcgctgtcc
     1321 aagccttgag caaatggctt ttgccacgcc cctctgcgcc ccacacatag accaaacgct
     1381 cgtgctggtg ttgtaaaacc tgcaaaagtt cacggttggc ctgccctaga aatttggcaa
     1441 aattggggta agcttggttg tcaaaattga aaatcaattg cgacactgaa gtggtttcgg
     1501 tctgatggtt ggggataagg ccattgtaac gtgctgccac agcttcgggc aaccgacagc
     1561 acgttatcag ggcttacagg ccgcttattc ttgattgtgg cgttggtaat aagggctgtt
     1621 gaaatagtgt tgggtcaatt ctcgtagcaa gaccaagcaa atggcggcca taggcaaggc
     1681 caacaacata ccgacaaaac ccaataattg gccaaatgcc attaaggcaa aaatcaccca
     1741 cagtgctgac aaacctatgc gttcacccac caatcggggc gtaatcacat agctttccaa
     1801 catttggccg accaagaaca ccgaccaaac cgacaccaag ccacccaaag tcccgaattg
     1861 caacaatgct gccaaagtcg ccaataacat gcctatgaat gcacccaaat acggtatcac
     1921 caccaacatg ccagcaagca tgcctatggc aaagcccgaa tccaagccta ccaaagccaa
     1981 accgccacca taaatcaaac ccacaatgat catcaccagc aattgaccac gcaaatactc
     2041 gctcagggtt ttgtctatct cgccaccaat gcgtaaaacc tgctcaaggt aacgacgcgg
     2101 aatcaaacgg cgcgtgcctt cagcccaacg cggccaatcg gccaagaagt aatacaacaa
     2161 aatgggcagc aaaaacaaat tggcagccat cagcatcaat gaggtgcctt tattggccaa
     2221 aaatggcatc aatttgctca agccactgcg tatttcctcg ctgttttgag tcacgtactg
     2281 ggtaaccact tgcccattca ggctcaaatg ggtatggaag gtttggttaa tccatggaat
     2341 caactggtgt tgtacataat tgaccatgtc aggaaccctt tgccaagctg cctcggcctg
     2401 ttgcaccatc atcggaatga tgaacaaaat cagcccaacc acaatggcaa tcgccaacaa
     2461 agtcaccaaa accgcggcca agccacgtcc tattttggca ctggtcagtt tgttcaccaa
     2521 aggattgagg atgtaagcca aaatggcagc tgcgataaac ggcgtcaata tggtgcgtaa
     2581 actgtacagc aacaccatca ccaccaagaa aaccaaactg cctatgatcc aatgcacata
     2641 gggattgcga ggacgcacgt acatgggcat gcctttatat cagtgttaaa aaaccgtttg
     2701 catcgtatca caatgcaaac ggttttagac aaacatctct attagttcat ggagattnnn
     2761 nnnnnnnnnn nnnaggccgc catcaattga cgcaacacat aatgcaaaat accacccgcc
     2821 ttgaagtatt ccacctcgtt caaggtatca atgcgacaca aaacctcggt ttgtgtttgg
     2881 ctgccatctt cccgtgtgat gtgcagggtc aatgtttgtt gtggcttgat gtcatcactc
     2941 aaacccgtga tgctgacttg ctcgcggcca gttaaaccca agctgctgcg gctttcacca
     3001 ttgagaaact gtaaaggcaa cacacccatg cccaccaagt tagaacggtg gatgcgctca
     3061 aaactctcag caatcaccgc tttcactccc agcaacaaag tgccttttgc tgcccagtct
     3121 cgtgaagagc ctgtgccgta ctctttgcct gcaaaaatta ccaatggtgt tttttcggct
     3181 tggtaacgca tggcgacatc gtaaatagcc tcttgcttgc cactaggcac atgcagactg
     3241 aaaccacctt ctaccccatc caacatttca ttcttaatgc ggatgttggc aaacgtgccg
     3301 cgcatcatca cttcatggtt accacgacgg gaaccataag agttgaaatc aatcggtgcc
     3361 acgccttttt cttgcaaata gcgccctgca ggagaatcgg ctttgatgtt acctgctggt
     3421 gaaatgtggt cggtggtgac tgaatcaccc aaaaccgcca acacgcgtgc acctgcgatg
     3481 tcggtaatgc tgcgcgttgg ttcggcaatg tgttcaaaaa atggtggatg ctgaatgtag
     3541 gtagaatcga gattccaatc gtaggtttgg ctttctttca ctgcgatggc ttgccaagtg
     3601 gcatcgccat caaacaccgc tgcatattct ttgcggaaca tggcagtgtc tactttcgcc
     3661 accgcctctg caatttctgc cgagctgggc cacacatctt tcaaatagat gtcttcacct
     3721 tgtgcatttt gccccaatgg ctctttgtcc aaatcaattt tgacactgcc tgccaatgca
     3781 taggccacca ccaatggtgg cgaagccaac cagttggttt tcaccaatgg atgcacacgg
     3841 ccttcaaagt tacggttgcc cgacaaaacg gaggccacgg tcaaattgcc ttggttaatt
     3901 gcttcttcaa tttctttggg caatgggcct gagttaccaa tacacgtggt acagccatag
     3961 cccaccaaat taaagcccaa ttggtttaag gcatcggtca ggcctgcttt ttgcaggtaa
     4021 tcggtcacta cttttgagcc aggcgccaag gaggatttca cccaaggctt gcgctgcaag
     4081 cctttttcta aggctttttt cgccaccaag ccagcggcca tcatcacgct tggattggag
     4141 gtattggtac atgaggtaat cgccgcaatg accaccgcac catttttcaa ttgcaaatct
     4201 tggccatcta ccgagcaagc aatgctcggc tcttcttcca atgattcttc tgttcccact
     4261 gcggcacctg caccaccttc gcctatcaaa cgagccaaat ctttcccgct cacaggtgcg
     4321 ctgctgtcaa caaactgttt gaatgcttgt ggtacttgcg acaacaccac tttgtcctgc
     4381 gggcgtttcg ggcctgccaa gctggcttct accatgccca tgtccaattc caatacattg
     4441 gtaaacacag gttcatcgcc tgtgttgcgc cacaaacctt gttgtttgca atacgcttca
     4501 accaaggcta tgcgtgcttc ttcacggcca ctcaagcgca gatagcccaa agtcacctcg
     4561 tctacaggga agaaaccaca ggtcgcacca tattcaggtg ccatattggc gatggtggca
     4621 cggtctgcca atggcaaatc ggccaaacca tcaccaaaga attccacaaa cttgcccacc
     4681 acaccttttt ggcgcagcat ttgggtcacg gttaaaacca aatcggtagc ggtaatgcct
     4741 tcacgcaatt tgccagtgag tttaaaaccc actacctcag gaatcagcat ggacacaggc
     4801 tggcccaaca tcgcggcctc agcttcaatg ccgcccacc
//
LOCUS       DAOMVK010000129         4829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_4970_length_4829_cov_2.036866, whole genome shotgun sequence.
ACCESSION   DAOMVK010000129 DAOMVK010000000
VERSION     DAOMVK010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4970_length_4829_cov_2.036866"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="PK856_05570"
     CDS             complement(<1..867)
                     /locus_tag="PK856_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HRL01873.1"
                     /translation="MLLSGLKLSKVGANPSTARQLESAKEALLGLFRVHGYELVSPPL
                     LEYTSSLLLDIDDGLALKTVRIVDQISGRQLGVRADITPQVARIDAHLLAGNDGINRL
                     CYAGSTVHARPDSLLSTREPLQIGAELYGYAGIEADVEIIDLMLKTLSLAHAQDVYLS
                     LGHVGIFQALADKAALTQAQRDSLLYLMKDKDSEAVLEAMQAWDIDTIWKETFAVLPT
                     LYGDVETVLRRAREDLPAIKGVQDALDALKSICKQFKAYKVHIDLSILRNDSYHTGLL
                     FAAYAPGWPDALA"
     gene            complement(917..1726)
                     /locus_tag="PK856_05575"
     CDS             complement(917..1726)
                     /locus_tag="PK856_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRL01874.1"
                     /translation="MNFFEAIVIAIVEGLTEFLPVSSTAHMIFTSSFFGIQEDAFVKM
                     FMVSIQLGSILSVVVMYWRKFFDKNNLGLYVKLACAVLPALILGKLFDDKIEAILGSP
                     RPIAVMLIIGGVILLFVDKWFTKPVVFSEKEVTIRKAVAIGFWQCLAMMPGTSRSAAS
                     IIGGMQQGLSRQAAAEFSFFLAVPTMLAVTTYSLFVKEWGTEGQGLTGIEMMMATPQN
                     LPIFLVGIVVAFLVALVAMKFFIGIIKKYGFKPWGWYRIIAGVILFTYFSL"
     gene            complement(1799..2485)
                     /locus_tag="PK856_05580"
     CDS             complement(1799..2485)
                     /locus_tag="PK856_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HRL01875.1"
                     /translation="MLFKKLMAAAVMTVGMVGASQAALVEGTDYVVLPEAIPQLQQDK
                     IEVLEFFGYFCIHCKNLEPTMAAHVKTFPTDTYFRAEHVVWRPEHLGLARIAAAVNAT
                     GMKAQANPVIFKAVMDEGVDLSNPETFKQWAAQQTAFDGQKLLAAFNGFQNQTEAKNM
                     AELSTKYKIQGTPTIIVGGKYQLQSQDMGKLDELVQKVRTERGMPAAQAKPVLKVKAT
                     GSRLATQANQ"
     gene            complement(2508..3296)
                     /locus_tag="PK856_05585"
     CDS             complement(2508..3296)
                     /locus_tag="PK856_05585"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRL01876.1"
                     /translation="MAKKTVQRQSKSKSLGQTLNGILIGLILGAIIIAILLWFLNKQK
                     MPTEPQKASEPTQTEIIVPNKAELPQTEPALTASETDTTEGTVGAASEVDNGESQKPV
                     IPKVPVTKPTESKVQSGEKKPQVPTHPEKKPDSKPTEHKTNTKPTPEQILEHGSVEKA
                     RAANQKEAAKKQAEKAPASTETQASSGRTVKLQAGSFGSKEQAEAQRAKLALLGISAQ
                     VETAQVNGKSVHRVRTARLNENKAAQVRKQLSNNGIDSIAVGAN"
     gene            complement(3353..>4488)
                     /locus_tag="PK856_05590"
     CDS             complement(3353..>4488)
                     /locus_tag="PK856_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HRL01877.1"
                     /translation="MAVACAQSHRAFVLPSESAEQAALLEQINVYAADSLSSVAAHLN
                     RVRLLDAVMPVEVVVDLTNLADLSEVKGQYSARLALEVAAAGGHNLLMMGPPGTGKSM
                     LAQRLPSILLPLTLDERLSVASIRSLLLQHSHEVLPQVRPFQMPHHSASAVALVGGGS
                     DPRPGAISLAHCGVLFLDELPEFDRKVLEALREPLEGGEIHIHRANRNVVFPARFQLV
                     AAMNPCPCGYYGHASKPCRCSVETIRRYRDKISGPLLDRIDLIIDVPQLTEHDLTNNK
                     RPECSDVVRERVLSAHAKQMQRQGKSNALLNVDELEQVADIATEATQYLSKLMNDLNL
                     SARSNHRLLRVARTCADLQADAVVKTGHIVEAIQFRRAWTNPL"
     assembly_gap    4489..4498
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4499..>4829)
                     /locus_tag="PK856_05595"
     CDS             complement(<4499..>4829)
                     /locus_tag="PK856_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HRL01878.1"
                     /translation="SGMNAPLVEVEAHLANGLPAFNIVGLPDTEVKESRDRVRAAILQ
                     SGFDFPSKKITVNLAPADLPKDSGRFDLPIAIGILIASGQIMADDVAQYEFAGELALS
                     GRLRPIRG"
BASE COUNT         1255 a         1282 c         1024 g         1258 t
ORIGIN      
        1 cgccaaggca tctggccaac ctggcgcata agcggcaaac aacaagcccg tgtggtaaga
       61 gtcattgcgc aaaatcgaca aatcaatgtg cactttatag gctttgaatt gtttgcaaat
      121 gcttttcaag gcatccaatg cgtcttgtac acctttgatg gcaggcaaat cttcgcgcgc
      181 acggcgcaat accgtttcca catcaccata caatgtaggc aataccgcga aagtttcctt
      241 ccaaatggtg tctatgtccc acgcttgcat cgcttctaat accgcttcgc tgtctttgtc
      301 tttcatcaaa tacaataagc tatcgcgctg cgcttgagtc aaggccgctt tgtcagccaa
      361 ggcttggaat atgcccacgt ggcccaaaga caaatacaca tcttgggcat gcgccaaaga
      421 caaggttttc aacatcaaat cgatgatttc cacatcggct tcaatgcctg cataaccata
      481 caattcagca ccaatttgca aaggctcacg cgtactgagc aagctgtctg ggcgtgcatg
      541 cacggtagaa cctgcataac aaaggcggtt gatgccatca ttgcctgcca acaaatgcgc
      601 atcaatgcgc gccacttgtg gggtaatgtc agcacgcaca cccaattggc gaccactgat
      661 ttgatcgaca atgcgcacgg ttttcagcgc caagccatcg tctatgtcca ataataatga
      721 tgaggtgtat tcgagtaagg gtggactcac gagctcatag ccgtgtaccc taaaaagacc
      781 taaaagtgcc tctttggcac tttccaattg ccgtgcagta gacgggtttg cacccacctt
      841 cgacagtttt agtcctgata gtaacacaaa ctgcaaaaat ccgcagcaaa aacctgtatg
      901 tacaggattt ttatgtttac aaactgaagt atgtaaacaa aataacgcca gcaatgatgc
      961 ggtaccaacc ccaaggtttg aaaccgtatt tcttaatgat gccaatgaag aatttcatcg
     1021 ccaccaacgc caccaaaaat gccactacga tgccaaccaa gaaaattggt aaattttgcg
     1081 gtgtcgccat catcatttcg atgcctgtta aaccttgtcc ttccgtaccc cactctttca
     1141 caaacaatga gtaagtggtc actgccaaca tggtgggcac agccaagaaa aaggaaaatt
     1201 ctgccgcagc ttgacgcgac aaaccttgct gcataccacc gatgatggat gctgcagaac
     1261 gacttgtgcc tggcatcatc gccaaacatt gccaaaaacc aatcgccacc gctttgcgta
     1321 tggtgacttc tttttcagaa aacaccacag gcttggtaaa ccatttgtcc acaaacaaca
     1381 agattacccc accaatgatg agcatcaccg caatcgggcg cgggctgccc aaaatggctt
     1441 caattttgtc gtcaaacaat ttgcccaaaa tcaatgcagg cagaacggca caagccagtt
     1501 tcacatacaa gcccaaattg tttttatcaa aaaacttgcg ccaatacatc accaccaccg
     1561 acaaaatact gcccaattgt atcgatacca tgaacatttt cacaaaagca tcttcttgta
     1621 tgccaaagaa agagctggtg aatatcatgt gcgcagtaga agaaacgggt aaaaactcag
     1681 tcaacccctc gacgatggcg atgacaattg cttcaaaaaa attcatttat tatttaactt
     1741 tcaaaagcgg cctgtaacaa caaacaggca gcctatagct gcctgttttt aacttacctt
     1801 actgattggc ttgtgtggcc aagcgtgaac ctgtggcttt gactttcagc actggcttgg
     1861 cttgggctgc tggcatacca cgctcggtac gcactttttg caccaactca tctaacttgc
     1921 ccatgtcttg tgattgcaat tggtatttgc caccgacaat gatggtagga gtgccttgga
     1981 ttttgtattt ggtagacaat tcagccatgt ttttggcttc ggtttggttt tggaagccat
     2041 tgaaagccgc caacaatttt tgaccatcaa atgccgtttg ttgagccgcc cattgcttaa
     2101 aggtttctgg gttactcaaa tccacaccct catccatcac agccttaaag atcacaggat
     2161 tggcttgggc tttcatgcct gtggcattca cagccgccgc aatgcgcgcc aaacccaaat
     2221 gttcaggacg ccataccacg tgttcagcac ggaaatacgt atcggttggg aaggttttca
     2281 catgcgcggc catcgtaggc tctaagtttt tacaatggat acaaaaataa ccaaaaaact
     2341 ctaacacttc aatcttatct tgttgcaatt gcggaatggc ctctggcaaa acaacgtaat
     2401 cggttccttc taccaaagca gcttgcgatg cacctaccat accgactgtc atcactgctg
     2461 ccgccatcaa ttttttaaac aacatacaca ttccttattt taaatgacta atttgcacca
     2521 acggcgatgg agtctatgcc attattgctt aactgtttgc gcacttgcgc ggctttgttt
     2581 tcattcaatc gggcggtgcg aacacggtgt accgatttgc cattcacttg cgcggtttcc
     2641 acttgggcag aaatgcctaa caaagccaat ttggcacgct gcgcttctgc ttgctctttg
     2701 ctgccaaatg aaccggcctg taatttcacc gtgcgacctg atgaagcttg ggtttctgtg
     2761 cttgcaggcg ctttttcggc ttgctttttg gccgcttctt tttgattggc tgcacgcgct
     2821 ttttccacac tgccatgctc taaaatttgc tcaggcgtag gcttggtatt ggttttgtgc
     2881 tcagtaggtt tgctgtcagg ctttttctct gggtgtgttg gcacctgagg ttttttctca
     2941 cccgattgca ctttggattc tgttggcttg gtgacgggta ctttaggtat cacaggtttt
     3001 tgtgattcac cattgtcaac ctcacttgcc gccccgactg tgccttctgt ggtatcggtt
     3061 tcagatgccg tgagtgcagg ctcggtttgt ggcaactctg ctttatttgg cacaatgatt
     3121 tcggtttgag tgggttcaga agctttctga ggctcggttg gcattttttg cttgttcaaa
     3181 aaccacaata aaattgcgat gatgatggca cctaaaatca atccgattaa aatgccattt
     3241 aaggtttgcc ccaaagattt ggatttactt tgacgctgta ccgtcttttt agccatgtaa
     3301 tgaaactttc tcaacgctta agcgcataat gttaccaaga tttttgcccc aactaaagcg
     3361 gatttgtcca agcccgtcgg aactgtatgg cttcaacaat gtggcctgtt ttcaccactg
     3421 catctgcctg caaatcagca caggtacgtg ccacacgcaa caagcggtgg ttgctgcggg
     3481 ctgacagatt taaatcgttc atcaattttg acaaatactg tgtggcctcg gtggcgatat
     3541 cagccacttg ctccaattca tccacattca ataaggcatt gcttttgcct tggcgctgca
     3601 tttgttttgc gtgcgcactc aacacccgct cacgcacgac atcactgcat tcaggccgct
     3661 tattgttggt taaatcgtgc tcagtcaatt gcggcacatc aataatcaaa tcaatgcgat
     3721 caagcaatgg gcccgaaatc ttatcgcgat accgccgtat cgtttccact gagcaacgac
     3781 aaggcttgct tgcgtgccca taataaccgc agggacaagg attcatcgcc gccaccaatt
     3841 ggaaacgtgc aggaaacacc acattgcgat tggcacggtg tatgtggatt tcaccgccct
     3901 ccaaaggctc tcgcaacgct tccaaaacct tgcgatcaaa ctctggcaat tcatccaaaa
     3961 acaacacgcc gcaatgcgcc aaggaaatcg ctcctggccg cggatcagac ccacctccaa
     4021 ccaatgccac tgctgaagca gaatggtgtg gcatttgaaa cggtctgact tgtggcaaga
     4081 cttcatgact gtgttgcaac agcaacgagc gtatgctcgc cacactcaag cgctcgtcca
     4141 atgtcaatgg cagcaaaatg ctaggcaaac gctgcgccaa catcgatttg ccagtgcctg
     4201 gcggccccat catcaacaga ttgtgcccac ccgcagcagc cacctccaaa gctaggcgcg
     4261 cgctgtattg ccctttgacc tcgcttaaat ctgccaagtt ggtcaaatcc acaactacct
     4321 caactggcat cactgcatca agcaatcgca cccgattcaa atgcgccgcc acactgctca
     4381 aactgtcagc cgcatacaca ttaatctgtt ccaacaatgc cgcttgctct gcactttcac
     4441 tcggcaacac aaatgcccga tgtgactggg cacatgccac tgccatggnn nnnnnnnncc
     4501 ctcgaatggg gcgcaagcgg cctgaaagag ccagctcacc tgcaaattca tactgcgcca
     4561 catcatcggc catgatttga cccgatgcaa tcaaaatacc gatggcaatc ggcaaatcaa
     4621 agcggcctga atctttgggc aaatctgcag gtgccaaatt cacggtgatt tttttggaag
     4681 gaaaatcaaa acccgactgc aagattgctg ccctaacccg atcacggctt tctttcacct
     4741 ccgtatcggg caagcccaca atgttaaacg caggcaagcc gttggctaag tgggcttcta
     4801 cctccaccaa aggtgcgttc attccgctt
//
LOCUS       DAOMVK010000130         4826 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_4973_length_4826_cov_2.731922, whole genome shotgun sequence.
ACCESSION   DAOMVK010000130 DAOMVK010000000
VERSION     DAOMVK010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4973_length_4826_cov_2.731922"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="PK856_05600"
     CDS             complement(<1..747)
                     /locus_tag="PK856_05600"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HRL01879.1"
                     /translation="MSIENSKLFQAFALTPDFNLRNRVVMAPMTTWSGNSDGTVSEQE
                     LQYLRRRVKGVGMVITGCTHVQENGIGFTHEFAGYDDKFIPSLSALAQAAKSGGAPAV
                     LQIFHAGNKAIPELIPNGDVVSACHLNTPSAAFHDSPVQSRALSEAEITEVIRAFGET
                     TRRAIEAGFDGVELHGAHGFLIQNFFSPHYNQRQDDWGGSLVHRMRFPLAVVEEVQRI
                     IRKHAKRPFLLGYRISVEEYEENGLRIADSL"
     gene            complement(877..>1741)
                     /locus_tag="PK856_05605"
     CDS             complement(877..>1741)
                     /locus_tag="PK856_05605"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRL01880.1"
                     /translation="YFPPAFDANKKYPAIVSAHPIGSCKEQTSGNVYGLALAAAGFVV
                     IAFDASFQGESGGEPRYVEDPSLRVADFSVVIDHLVTLNYVDENRIGVLGICGGGGYS
                     INAAMTERRIKAVGTITGANYGRVMREASAGNPIAFLEAIAAQRTAEARGAPVRVDQG
                     LYPTVEAAQADHADIDPLEATEYYRTARGQKPNGVNKTVFSRNAVAVGWDAFHLAEVL
                     LTQPLMIVVGSKPGGFGAYRDGFEIINRAASTNKELVVVEGWSHYDLYDKPEPVKVAL
                     DKLIPFYQANL"
     assembly_gap    1742..1751
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1872..2552)
                     /locus_tag="PK856_05610"
     CDS             complement(1872..2552)
                     /locus_tag="PK856_05610"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRL01881.1"
                     /translation="MNVLHLDSSGRVGFGGIAPHGAYGSYSRRLSQHFVHTWQNLDGN
                     TIIKYRDLGLYPPTPVDQGWIEGAFSQQELSDTHANKMSESDELIEELLWADIVVIGA
                     PMYNFNVPANLKAWVDNIVRVRKTLVSDPEDPSNPYSPVFKNRRLPVMLLTARGDHGM
                     DEGEEFAHINHLDPSMRTALGYIGLEEVYSIAIEYTAVGGAELDQSVEQALSQATRRA
                     EQLFQELA"
     gene            complement(2555..3484)
                     /locus_tag="PK856_05615"
     CDS             complement(2555..3484)
                     /locus_tag="PK856_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRL01882.1"
                     /translation="MEKIFIQNLNGGDIKIAAHVYFPNNFDPTQRYPAIVVSHPGGGV
                     KEQASGLYAQKLAAAANFITIAYDASFQGESTGLPRQLENPHVRTEDISAVIDYLTTL
                     AYVDAQNIGAIGICAGAGYSANAAINDRRIKALGTVSMVNIGQMFRNGWENTVQDHDT
                     LPLLAMGSAARSEDAQAMDLQALPLAPMTKEDAPNPELEEAWEYYHTPRCEHPNAPGF
                     MTTRSLTQIIAYDAYHEAEAFLTQPIFSVIGEHAGSAWMTHNLHQRARSEQKSIQVIQ
                     GANHMSLYDREPFVAEAVAGLTQFFKTFLNTEA"
     gene            3612..4523
                     /locus_tag="PK856_05620"
     CDS             3612..4523
                     /locus_tag="PK856_05620"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRL01883.1"
                     /translation="MIIDPSLLPSLAWFLVIAKHQSFTKAANEMDVSRAALSQHLKSL
                     EQRLGVRLINRTTRDMSLTEAGLELFSVLQPAMDNIQTVVHGLMDAQSEPTGVLKIDT
                     SRTAARLLLNPVIEVFLQRHPKLQLELVINDGLSNIIVSGVDAGIRLGASVDNGMVAI
                     PITRPIQMAVVGSEDYFKRHGIPETPDDLVRHNCLAYRFSSSGSLDMWRFTSPADGQQ
                     ELIFEPKGNAIFNDDESMLQAALQGVGVMKHLDVCVEQYIQSGQLKRVLASWCKPFPG
                     FYLYVPTRAQMPAKTRALMDFLVAQRT"
     gene            complement(4717..>4826)
                     /locus_tag="PK856_05625"
     CDS             complement(4717..>4826)
                     /locus_tag="PK856_05625"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009426695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRL01884.1"
                     /translation="DWGHMIKGHGGMMDRVDSICFAAPIFFHIVRYFWA"
BASE COUNT         1218 a         1142 c         1181 g         1275 t
ORIGIN      
        1 caagctgtct gcaatgcgca agccattttc ttcgtattct tcaacagaga tgcggtagcc
       61 cagtaagaat ggtcgtttgg catgcttacg tatgatccgt tgcacttctt ctaccaccgc
      121 caatgggaaa cgcatgcgat gcaccagcga acctccccaa tcgtcttggc gctgattgta
      181 atggggcgaa aagaagtttt ggatcaaaaa tccatgtgca ccatgcaatt caacgccatc
      241 aaatcccgct tcaatcgctc ggcgtgtggt ttcaccaaat gcccgtatca cctcggtaat
      301 ttcagcctca cttaaggcgc gactttgaac agggctgtcg tgaaaagcgg cagagggcgt
      361 attcaaatgg caggcactga ccacatcacc attcggaatg agttcaggaa tggccttatt
      421 gcctgcatga aagatttgca ataccgctgg agcaccgcca cttttcgcag cttgtgctaa
      481 tgcgctcaag ctgggaatga atttgtcatc atagcccgca aattcatggg taaaccctat
      541 gccattttct tgtacatggg tacagccagt gatgaccatg cccacaccct tgacgcggcg
      601 acgcaggtat tgcaattctt gctcagacac agtgccatcg ctgttgccag accaagtggt
      661 cattggggcc atcaccacac ggtttcttaa gttaaagtca ggggttaatg caaaggcttg
      721 gaacaatttg gaattttcaa tagacatgtt tcttcatcca agaggcgtga ttaaaaactg
      781 gcatgaaatc catccatgcc agtgatgtgc ttgagcagct caaataaggg ttgttttcaa
      841 atgcaagcag gcttacaggc cgctgtgaaa gagggcttag agattggctt gataaaaagg
      901 aatcagcttg tccaaagcca ctttgacagg ctcgggtttg tcgtacaaat cgtaatgtga
      961 ccaaccctct accaccacca attccttatt ggtagaagcc gcacggttaa tgatttcaaa
     1021 accatcacgg taagcaccaa agccaccagg tttgctgccc accacgatca tcaaaggttg
     1081 ggtcaacaac acctctgcca aatgaaacgc atcccaaccc actgccacgg cattgcgaga
     1141 aaagacggtt ttgttcacgc cattgggttt ttggccacga gctgtgcggt agtattcagt
     1201 ggcttccaat gggtcaatat cagcatggtc ggcttgagcg gcttcaactg ttgggtacaa
     1261 accttgatcc acacgtactg gtgcgccacg ggcctcagct gtcctttggg cggcaatcgc
     1321 ttccaaaaag gcaatcggat tgcctgcact ggcttcgcgc atcacgcggc cgtaattcgc
     1381 tcctgtaatt gtgcccactg ccttgatacg acgttctgtc atggccgcat tgatggaata
     1441 gccgccacca ccacaaatgc ccaacacgcc aatgcggttt tcatcgacat agttcaaagt
     1501 caccaaatgg tctatgacca cactgaagtc agctacacgc aaacttgggt cttcaacata
     1561 gcggggctca ccaccgcttt cgccttgaaa actggcatca aaagcgatga cgacaaaacc
     1621 agcggctgcc aaggctagtc catacacatt gcccgatgtt tgctctttgc agctgcctat
     1681 ggggtgtgcg ctcacaatgg cagggtattt tttgttggca tcaaatgctg gcgggaaata
     1741 cnnnnnnnnn ntgaatataa aaaccaagta gtataaaaac catttccaat aagtgttttt
     1801 gattttaatg gattccatga tttcacctcg tgatgattca aattaataaa gaaatattgt
     1861 tgtaatgtaa gttaagccaa ttcttgaaac aactgttcgg cacgtcgagt cgcttggctc
     1921 aaagcctgct caaccgattg gtctaattca gcgccaccca ccgcagtgta ttcaatggcg
     1981 atgctgtaaa cctcttcgag gccgatatag cccaatgcgg tgcgcatgct tggatccaaa
     2041 tggttgatgt gggcaaactc ttcaccttca tccatgccat ggtctccacg cgctgtgagc
     2101 aacattacgg gcaaacgacg gtttttaaac acaggagagt aagggttgct tgggtcttct
     2161 gggtctgaaa ccaaggtttt gcgtacccgt acaatgttgt ccacccacgc ttttaagtta
     2221 gcgggtacgt tgaagttgta cataggggca cctatgacca cgatgtcagc ccacagcaat
     2281 tcttcaatca actcatcgct ttcactcatt ttgttggcgt gggtgtcact caactcttgt
     2341 tgagagaatg caccctcaat ccagccttga tccacagggg tgggtggata caagcccaaa
     2401 tcacggtatt tgataatggt gttgccatcc aagttctgcc aagtgtggac aaaatgctgt
     2461 gacaaacggc gagagtaaga accataagcg ccatgtgggg cgatgccgcc aaagcctaca
     2521 cggccgctgc tgtctaagtg taatacgttc atagttatgc ctcagtgttt aagaatgttt
     2581 taaaaaattg ggtcaaacct gcaactgcct cagcaacaaa aggctcacgg tcatacaaag
     2641 acatgtggtt tgcaccttgt atcacttgaa tgcttttttg ttcgctgcga gcgcgttggt
     2701 gtaaattgtg ggtcatccaa gcactgcctg catgttcacc aatcacactg aaaataggct
     2761 gagtcaaaaa tgcctctgct tcgtggtaag catcgtaagc aatgatttga gtcaggctgc
     2821 gtgtggtcat gaagccaggt gcattgggat gttcacaacg tggtgtgtgg taatattccc
     2881 aagcctcttc taactcaggg ttaggagcgt cttcttttgt cattggtgcc agtgggagcg
     2941 cttgtaagtc catggcttgg gcatcttcac tgcgagccgc tgagcccatt gccaacaaag
     3001 gcaaagtatc atggtcttgc acggtgtttt cccaaccatt acggaacatt tggccaatat
     3061 tgaccatgct gaccgttccc aaagccttga ttcgtctgtc gttgatggct gcatttgccg
     3121 agtaacctgc tccagcacaa attcctatgg cgccgatgtt ttgcgcatcc acataggcaa
     3181 gagtggtcaa ataatcaatg acggcgctga tgtcttcggt tctgacatgt ggattttcca
     3241 gttggcgtgg taagcccgtg ctttcacctt ggaaagaagc atcgtaagca atggtgatga
     3301 aattggcggc agccgccaat ttttgtgcat acaagcccga tgcttgttct ttaaccccgc
     3361 caccaggatg cgaaaccact atggctgggt agcgttgtgt tggatcgaaa ttgtttggaa
     3421 agtaaacgtg tgccgcaatt ttgatgtcac cgccatttaa attctgaatg aaaatctttt
     3481 ccatggtctt tctcctgtta aatcaatttg tttggatgtg tttctttgtt cgatgtgaag
     3541 catcatacgg gtgttttatt tgcagatata tggggttaga atgacatctt tgttcatgat
     3601 tacttaacaa tatgatcatc gatccctcgt tgcttccctc attggcttgg tttctcgtca
     3661 ttgccaaaca ccagagtttt accaaggcgg ccaatgaaat ggatgtgtcc agagcagcct
     3721 tgtcgcagca tttgaaatca ttggagcagc gtttgggcgt gcgcctcatc aatcgcacca
     3781 cccgtgacat gtctctgact gaggctggct tggaactctt cagcgtttta cagcccgcca
     3841 tggacaacat tcaaaccgtg gtgcatggct taatggatgc ccaaagcgaa cccactggtg
     3901 tcttgaaaat agatacctcc agaaccgcgg caaggctgtt attgaatcct gtgatagaag
     3961 tgtttttgca gcgccatcca aaattacaat tggaattggt catcaacgat ggcttgtcta
     4021 atatcatcgt cagtggcgtg gatgcaggca tacgtttggg cgccagtgtg gacaatggca
     4081 tggtggcgat accgattacc cgtccgattc agatggcggt ggtgggctct gaggattatt
     4141 ttaaacggca tggcatacct gaaacgccag acgatttggt gcgacataat tgcttggctt
     4201 accgtttttc cagcagtggt tctttggata tgtggcgctt cacatcgcca gcagatgggc
     4261 agcaagagtt gatttttgag cccaagggca atgccatttt caacgacgac gaaagcatgt
     4321 tacaggccgc cttacaaggc gtgggggtga tgaagcattt ggatgtttgt gtggagcaat
     4381 acatacaaag cggtcaattg aagcgggtgt tggcctcgtg gtgcaaacca tttccaggtt
     4441 tttacctgta tgttcccaca cgagcgcaaa tgcctgcgaa aacccgcgct ttgatggatt
     4501 tcttggtggc acagcgaact tgataaagaa agtagtgtga gattggccag tgatgtagag
     4561 ctagggcatg gatggatgtt gggcaggttt gggcagtgtg tactttgcac gtaaacacac
     4621 aatacagagt gaaaatggct taggaacatc tcatgccaaa cataagcggc ctgtaaacag
     4681 acaaaaactg tttacaggcc gcttattgtc gctgcatcaa gcccagaaat agcgcacgat
     4741 gtggaagaaa attggggcgg caaaacaaat tgaatcgacc ctgtccatca tgccgccgtg
     4801 gcctttgatc atatggcccc agtctt
//
LOCUS       DAOMVK010000131         4819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_4981_length_4819_cov_2.736356, whole genome shotgun sequence.
ACCESSION   DAOMVK010000131 DAOMVK010000000
VERSION     DAOMVK010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4981_length_4819_cov_2.736356"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1394
                     /locus_tag="PK856_05630"
     CDS             <1..1394
                     /locus_tag="PK856_05630"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HRL01885.1"
                     /translation="FDIFKVGLGPSSSHTVGPMKAAAAFVQQLGGLNALQLTGRIAVE
                     VYGSLALTGEGHGTFEAILLGLEGEMPDSIDLNSIPERLAHINSQASLPLDGKHEIEF
                     IVARDIVIRYAESLPKHPNGLRFTAYDTNEKQILQQVYYSVGGGFIVTDEEFDLKLDS
                     GVEVPYPFHNAQSMAEQCVAHNISLSELILANEMALQAADADTVKQRVRAVAQVMFDC
                     VERGLHQTGELPGGLKVKRRAPYLAEKLSDLREANQINTQLWPMVFAMAVNEENAAGG
                     RVVTAPTNGAAGIIPSVLTYFQRFHPMNSEDGIVQFMLTAAAIGMLYKMNASISGADV
                     GCQGEVGVACSMAAAAYCDVSGGTIWQIQNAAEMAMEHHLGLTCDPVGGLVQIPCIER
                     NGIAAEKAIKLGALALLEDGLDKKVSLDTVIATMLQTGRDMKHIYKETSLGGLAVSMK
                     HKMVPVSVSVIEC"
     gene            1448..1771
                     /locus_tag="PK856_05635"
     CDS             1448..1771
                     /locus_tag="PK856_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01886.1"
                     /translation="MDTHYYSVSLASGEHLGFLVTVLDGEDQAEASGQAVLKIQPSEA
                     AWLKRPESLSLQALSAYQPLHWQHGQDGITLHDADSNPLTRMSDGYFKWQGYVYVVND
                     ISSVM"
     gene            1773..1988
                     /gene="ccoS"
                     /locus_tag="PK856_05640"
     CDS             1773..1988
                     /gene="ccoS"
                     /locus_tag="PK856_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HRL01887.1"
                     /translation="MESVFILIPISIILVFVIGGFFWWSSKNGQFDDLEGPAHRILLD
                     DDSPQVTDNPQQGVDTPPHSNQSTNQP"
     gene            2027..3319
                     /locus_tag="PK856_05645"
     CDS             2027..3319
                     /locus_tag="PK856_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL01888.1"
                     /translation="MFAFARTRRFAPLFGTQFLGAFNDNLFKTSLVTLISFYGLGKET
                     GLRPEILVAISTLLLVLPYFLFSSISGQMSTRWNKASIAKGVKIGEVLIMLLAAYGFI
                     TESVWTLMGCLFLMGIHSTVFGPVKYAILPEYLRPNELLGGNSLIESGTFLAILFGQI
                     IGAIMVGGGVYTVIAITVLVAALGTYTSMYMPATPPQDPSQKVQLNIWGSTRDLLKKA
                     AAITPVKTSILGISWFWLMGSVYTTQLSTFAKHYLGDSEALFSVLLAMFSIGIGIGSI
                     LCAKISHNRLNLRLVLVGASGMAVVGVLLAVYTFGLPAANPNTTLWQFLSTGAHYYLL
                     FLLVLIGFFGGFFSVPLYTWLQTSSSDSFRAQAVAANNIINGLFMVIGAIVSMVILFA
                     TDSLGLLFLLLALGNLGMIYYLTRLSPDLLGLQHDETE"
     gene            3330..4661
                     /locus_tag="PK856_05650"
     CDS             3330..4661
                     /locus_tag="PK856_05650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HRL01889.1"
                     /translation="MKTQWACVLCLCTSLSMNAQAFDLQAAWDAALGYDASHSAAAYQ
                     RLAAQENIPQARAALLPQVSLNGSYQRNYPIEPDADSNNSYGYSVQATQILFDASKYA
                     DYKQGKLAAKLADVQFDLSEQQLLLNVSQAYFDVLTAYDLISASKASKILYERQLEQA
                     KTMFDVGAATIVDTYEAQAGLDNAQVQVLNAEQKLHLAQQKLEYYTGLNPLDIERIDG
                     KQVNRILNRKTLAEWQQLAYLSNTNISAKHLAVEQAQAAVQKAKANRYPTVELQAAYQ
                     DQHQQVSNMGFSENVHNKGASIGVNLSMPLYTGGALSSQVRQTQFELLQREDELTASK
                     RDTDLQVREQYLNVQSGLAQVNALEQLVLTNRKKLESSQLGQQVGVRSNLDVIDAQRT
                     LAESEQQLAKARYDYLQAQLALAQAAGQLQTGEALRQINDAIRQQPLRKTP"
BASE COUNT         1194 a         1229 c         1170 g         1226 t
ORIGIN      
        1 tattcgatat ttttaaagtg ggcttgggcc catccagttc ccataccgtt ggccctatga
       61 aggcggcggc tgcctttgtt caacaattgg gcggcttaaa tgcattgcaa cttacaggcc
      121 gcattgcggt tgaagtgtat ggctctttgg ccttaaccgg tgaaggtcat ggcacgtttg
      181 aagccatttt attgggccta gaaggcgaaa tgcctgacag cattgatttg aacagcattc
      241 cagaacgctt ggcccacatc aacagccaag ccagcttgcc tcttgatggc aaacacgaaa
      301 ttgaattcat tgttgcccgt gacatcgtga tacgttatgc agagagcctg cccaaacacc
      361 ccaatggctt acgctttacc gcatacgaca ccaatgaaaa acaaatctta caacaagtat
      421 actactcggt cggcggtggc tttatcgtca ccgacgaaga gtttgatttg aaattggaca
      481 gcggcgtgga agtgccctac ccatttcaca atgcccaaag catggccgaa caatgtgtgg
      541 cccacaacat cagcttgtct gaattgattt tggccaacga aatggcctta caggccgctg
      601 atgccgacac cgtaaaacaa cgcgtgcgtg ctgtggctca agtgatgttt gactgtgttg
      661 aacgcggctt gcaccaaacg ggtgaattgc caggcggcct gaaagtgaag cgccgcgcac
      721 cttacttggc tgagaaactc agcgatttgc gcgaagccaa ccaaatcaat acccaattgt
      781 ggcccatggt gtttgccatg gcagtcaacg aggaaaacgc tgcgggtggt cgtgtggtca
      841 ccgcaccaac caatggtgcc gctggaatta ttccttcggt tttgacctat ttccaacgct
      901 ttcaccccat gaactccgaa gatggcatcg ttcaattcat gctcacagcg gccgccattg
      961 gcatgttgta caaaatgaat gcatcaattt caggcgcaga cgtgggctgt caaggcgaag
     1021 tgggtgtggc ctgctctatg gctgccgcag cttattgtga tgtcagcggc ggtacgattt
     1081 ggcaaatcca aaacgctgcc gaaatggcaa tggaacacca tttgggtttg acttgcgacc
     1141 ctgtcggtgg cttggtgcaa attccttgta ttgaacgcaa tggcattgcc gctgaaaaag
     1201 ccattaagct cggtgctttg gctttgcttg aagatggctt ggacaagaaa gtgtcgctgg
     1261 acacggtgat tgccaccatg ttgcaaacgg ggcgcgacat gaaacacatc tacaaagaaa
     1321 cctctttggg tggcttggct gtcagcatga aacacaaaat ggtaccggtt tcggtttccg
     1381 tgattgagtg ctgagtcact cttaagtttg cggtatactg gcgttttcgt gatgtttgaa
     1441 tcggcatatg gacactcatt actacagcgt cagtttggcc agtggtgaac acttgggctt
     1501 tttggttacc gtattagacg gtgaagacca agccgaagcc tctggccaag cagttctcaa
     1561 aatccaaccc agtgaagccg cttggcttaa gcggcctgaa agcctgtctc tacaggcttt
     1621 aagtgcttat caacccttac attggcaaca cggtcaagac gggattacct tacacgatgc
     1681 tgattcaaat cccctcaccc gcatgagcga tggctatttc aaatggcaag gctatgtgta
     1741 tgtggtgaac gacatttcga gtgtgatgtg atatggaaag cgtcttcatc ctcatcccca
     1801 tcagcatcat cttggttttc gttatcggtg gttttttctg gtggtcgtct aagaatgggc
     1861 aatttgatga tttagaaggg cctgctcacc gcattttatt ggatgacgac agcccccaag
     1921 tcacggataa tccccaacaa ggtgtggata cccccccaca cagcaaccag tccacaaacc
     1981 agccttaaca ttgcccttac aggccgctca taccaatagg acacccatgt ttgcattcgc
     2041 tcgcacccgc cgcttcgccc cattgtttgg cacccaattc ttgggcgctt ttaatgacaa
     2101 tctgtttaaa acctctttgg tgaccttaat cagtttttat ggtttgggca aagaaacagg
     2161 cttgcgaccc gaaatcttgg tagcgatttc caccttgctg ttggtattgc cttatttcct
     2221 gttttcatcg atttctggac aaatgtccac acgatggaac aaggccagta ttgccaaagg
     2281 ggtcaaaatt ggtgaagtgc tcatcatgtt gttggctgcc tatggcttca tcaccgaatc
     2341 ggtgtggaca ctgatgggtt gtttgtttct catgggcatc cactccaccg tgtttgggcc
     2401 tgtgaaatac gcgattttgc ctgagtattt gcgcccgaat gaattgctgg gcggcaacag
     2461 cctgattgaa agcggtacat tcttagcgat tttatttggc caaatcatcg gtgccatcat
     2521 ggttggcggc ggcgtttaca ccgtgattgc catcaccgta ttggtggctg cgctgggtac
     2581 ttacaccagc atgtacatgc ccgccacacc gccgcaagac cccagccaaa aggtacaact
     2641 caatatttgg ggctctaccc gcgatttgct caaaaaagcc gctgccatta ccccagtcaa
     2701 aacctctatc ttgggcatct cgtggttttg gttgatgggc tcggtttaca ccacccaact
     2761 gtccaccttc gccaaacact atttagggga ctctgaagcc ttgttttcag tcttgttggc
     2821 catgttctcg ataggcatag gcataggctc aattttgtgc gcgaaaatct ctcacaaccg
     2881 tttgaacctg cgtctggttt tggtaggggc atcaggcatg gctgtggtag gtgttttgtt
     2941 ggcggtctac acttttggtt tgcccgctgc caacccaaac accaccttgt ggcaattttt
     3001 gtctacaggc gctcattact atctgttgtt cttattggta ctcattggtt tttttggtgg
     3061 cttcttttcg gtgcctttgt acacatggtt gcaaacgtcc agcagcgaca gcttccgtgc
     3121 ccaagccgtg gcggccaaca acatcatcaa tggcttgttc atggtgattg gcgccatcgt
     3181 cagcatggtg attttgtttg ccaccgacag tttgggtctg ctgttcttgt tgctggcatt
     3241 gggcaacttg ggcatgattt attacctcac ccgcttaagc cccgatttat tgggcctcca
     3301 acatgatgag actgaatgaa ttgcttccaa tgaaaacaca atgggcttgt gtgttatgcc
     3361 tctgtaccag cttgagcatg aacgcgcagg cttttgattt acaggccgct tgggatgccg
     3421 cgcttggcta tgatgcgtcc cattcagccg ccgcttacca acgtttggcg gctcaagaaa
     3481 acatcccgca agcccgtgct gccttattgc cacaggtatc gctcaatggc agctaccaac
     3541 gcaactaccc aatcgaacct gatgcagaca gcaacaacag ctatggctac agcgttcagg
     3601 ccacgcaaat cctgtttgat gccagcaaat acgccgatta caaacaagga aaattggccg
     3661 ccaaattggc cgatgtgcag tttgacttga gcgagcaaca attgctgctc aatgtcagcc
     3721 aagcctattt tgatgtgctc accgcctacg atttgatcag cgccagcaag gcctcaaaaa
     3781 tactgtacga acgccaattg gagcaggcca aaaccatgtt tgatgtgggc gccgccacca
     3841 ttgttgacac gtatgaggcg caagcaggtt tggacaatgc ccaagtacag gttttgaatg
     3901 ccgaacaaaa gctgcatttg gcccaacaaa aactggaata ttacacaggc ttgaatcctt
     3961 tagacattga gcgcattgat ggcaaacaag tcaaccgcat actgaatcgc aaaaccttgg
     4021 cagaatggca gcaattggcc tacctaagca acaccaatat cagcgccaaa cacttggccg
     4081 tagagcaagc ccaagcagct gtgcaaaaag ccaaagccaa tcgctacccc actgttgagt
     4141 tacaggccgc ttatcaagac caacaccaac aagtcagcaa tatggggttt tccgaaaacg
     4201 tgcacaacaa aggcgcttcc attggtgtga acctgagtat gccgctgtac acgggcggcg
     4261 ctttaagcag ccaagtgcgt caaactcagt ttgaattgct acagcgtgaa gacgagctca
     4321 ctgccagcaa acgcgacacc gatttacaag tacgtgaaca atacctgaat gtgcaaagcg
     4381 gtttggcgca agtgaacgcg ctcgaacaat tggtgctgac caatcgcaaa aaactcgaat
     4441 ccagccaatt gggccagcaa gtgggtgtgc gcagcaattt ggatgtcatc gacgcccaaa
     4501 gaaccttggc tgaatcagag caacagctgg ccaaagcccg ctatgactat ttgcaagcac
     4561 aattggcttt ggcacaagcc gccggccaat tgcaaaccgg tgaggctttg cgtcaaatta
     4621 atgacgccat tcgccagcag ccacttcgca agacccctta aaaagtggca taatcaatac
     4681 gccctgaatc agggcgttat tttttagggt gtgtgcttac aggccgcccc gacagtttga
     4741 atcacctaga aacacaggct aaataaccat gctgctaatg ctcgacaact atgacagttt
     4801 cacttacaac atcgtgcaa
//
LOCUS       DAOMVK010000132         4810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_4998_length_4810_cov_2.812618, whole genome shotgun sequence.
ACCESSION   DAOMVK010000132 DAOMVK010000000
VERSION     DAOMVK010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4998_length_4810_cov_2.812618"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /locus_tag="PK856_05655"
     CDS             complement(<1..1302)
                     /locus_tag="PK856_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01890.1"
                     /translation="MAADSKYLTGANKDRNFYDASDAASLTSAFDAILESIKRDNPRK
                     PQAVFSATSPAISSNYVDGLAAAATLNTGSWSSEIVFYDVDANGRLDTSKPKRATFGN
                     RKLLISNGTDVHLYPNTSSGYDNSWYDIANNSGKNTNEWRDGLLKWMARDGDDAAIKT
                     SNSNFILDYRVRPLANGTTTDQRSMGDIIDNSILAIGEMRNKRQEFVVTATNDGLVYV
                     FQSQDNKDSPYDLKFNYAPAKIERNSNDGSDYVGKYYKDTTREKYGDNSMTNPHRYLL
                     NGGMVARSTDTNGQGKQVFLASNMGQAGRGAFAINIGGVNRSTGQPIAANNVANNNWQ
                     TEVKLFETPSGSTNTLGYTIGSPQIGRVQVDTSSTAPLHDTGSHVRYGVFVSNGYNYK
                     TDYKDTNSAYADTPALYVYEGLGQDVGLQATSSSYTKGEVIK"
     gene            complement(1381..2022)
                     /locus_tag="PK856_05660"
     CDS             complement(1381..2022)
                     /locus_tag="PK856_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01891.1"
                     /translation="MRTKTISTGASRLCTTIKERQLRPLLTDEDREIKSWFGISENQA
                     FTCQTGNSNYTNPASITAKDNKRKHFTDDYELIRKHIACLSPGGGTPANERAKIVVPY
                     MVDSDNLKNRCQKSYLVMLSDGDASDSGQLSDLTMARYNNDQKTSGKDAYGISWNDFT
                     AGAPPVDYSKQMVNTFTIGFGSGLSKAGKKFLDQGASCIYTPPDVIGNVKMTP"
     gene            complement(2802..3299)
                     /gene="queF"
                     /locus_tag="PK856_05665"
     CDS             complement(2802..3299)
                     /gene="queF"
                     /locus_tag="PK856_05665"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003791471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HRL01892.1"
                     /translation="MSSRNAQELDGLTLLGNQNTQYPSEYQPSVLEAVPNLHTARDYF
                     VKFNCPEFTSLCPLTGQPDFATIYISYIPDEKLVESKSLKLYLFSFRNHGDFHEDCIN
                     KIMLDLISLLNPRYIEVWGKFTPRGGLSIDPYCNYGRPDSKYTQMAEHRLFSHDMYPE
                     KIDNR"
     gene            3484..3957
                     /locus_tag="PK856_05670"
     CDS             3484..3957
                     /locus_tag="PK856_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRL01893.1"
                     /translation="MSREQQWLEWAAKLDFDTHAAIKIGGNYVAVLRDGAQVYVSGQI
                     PRILDTVVVTGRAGEDVTLVQAQYAARVCAMRALSLLRQELGSLDAVQQVLRINVYVQ
                     SATDFTLQSEVADAASGVLFEVLGAAGRHTRTSVGVYQLPKNATVELDLVATVAA"
     gene            complement(4147..>4810)
                     /locus_tag="PK856_05675"
     CDS             complement(4147..>4810)
                     /locus_tag="PK856_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HRL01894.1"
                     /translation="QYDYSIDLSEDGQVSIAKRERGSPIDTLVSEMMILANSTWAKML
                     HEGERAAIYRVQPAGKVRMSTQSEAHIGMGVEHYAWCTSPLRRATDYFNQRQLLSLIE
                     PEHFTPRFDMQSSDVFALMRDFDTTYSAYADFQRKMESYWSLVYIQQQGLRHLKAVVL
                     KEDLVRIDGLPLVSRAVGVPVCMPRSRAWFNVTGIQLPLQTISLNFNKFIHSAEDETE
                     PA"
BASE COUNT         1205 a          973 c         1227 g         1405 t
ORIGIN      
        1 tttgatgact tcccctttgg tgtaactgct cgaagtggcc tgtaaaccca catcttgtcc
       61 caatccttca tacacataca aagcgggagt atcagcatag gcactgttgg tgtctttata
      121 atcggttttg tagttgtagc cgttgctgac aaacacgcca tagcgaacat gactacctgt
      181 atcatgtagg ggagctgtgc tgctggtatc aacctgtacc cgaccaattt gtggtgagcc
      241 tatggtatag cccaaagtgt ttgtgcttcc tgagggtgtt tcaaacagtt ttacctctgt
      301 ctgccaatta ttattggcca cattattggc ggctatgggt tgacctgtgc tgcgattcac
      361 cccacctatg ttaatggcaa atgccccccg tccagcttga cccatgttcg atgcaagaaa
      421 aacctgtttg ccctgaccat tggtgtcggt actgcgggcc accatgccac cattcaataa
      481 atagcggtgg gggttggtca tgctattgtc accatatttt tcgcgcgtgg tatctttata
      541 atatttaccc acatagtcgc tgccatcatt gctgttacgc tctattttgg ctggggcata
      601 attgaatttt aaatcgtaag ggctgtcttt gttgtcttgg gattgaaaca catacaccaa
      661 gccatcgttg gtagcggtta ccacaaattc ttggcgtttg ttgcgcattt caccaatggc
      721 taaaatggag ttatcaatga tgtcacccat tgaccgttga tctgtggttg taccattggc
      781 caagggacgt acgcgataat ccaatataaa gttggaatta ctcgttttaa tggcggcatc
      841 atcaccatct ctcgccatcc actttaataa gccatctcgc cattcattgg tgtttttacc
      901 gctattattt gcaatgtcat accaagagtt gtcatagcct gatgaggtat tggggtacaa
      961 atgcacatct gttccattgc tgattaacaa cttgcgatta ccaaaagtgg cccgtttcgg
     1021 cttactggta tccaagcgac cattggcatc cacatcataa aagactattt cactgctcca
     1081 agagcctgta tttaaagtgg ctgccgctgc caaaccatcc acatagttgg aactgatggc
     1141 aggtgaagta gcggaaaata cagcttgagg cttgcgtgga ttgtcccgtt tgatggattc
     1201 caaaatcgca tcaaacgcac tggtgagtga tgcagcatca ctggcatcat agaaatttcg
     1261 gtctttgttg gcacccgtga ggtatttact gtctgctgcc acacatttag gaattacagg
     1321 agaagaggag ataatttcac ctggtttggt ctcagtgtca gggtcaattt caaattgccg
     1381 ttagggggtc atttttacat tgccgataac atcaggaggg gtgtaaatac agctggcacc
     1441 ttgatccaag aattttttac ctgctttact taagccactg ccaaagccaa tggtaaaggt
     1501 gttgaccatt tgcttggagt aatcaacggg aggggcacct gcggtaaagt cgttccagct
     1561 gattccataa gcatctttac cactggtctt ttgatcgtta ttgtaccgag ccatagtgag
     1621 gtcacttaat tgtccgctgt cagaagcgtc accgtcagag agcatcacca aatagctttt
     1681 ttggcaacgg ttttttaaat tatcgctgtc taccatataa ggtacaacga ttttagcccg
     1741 ttcattggcc ggtgtacctc caccagggga taagcatgca atgtgtttgc gtatcaattc
     1801 ataatcatcg gtaaaatgct tacgcttatt gtctttagca gtaatgcttg ctggattggt
     1861 gtaattgcta ttgccagttt ggcaggtaaa agcctggttt tcactgatgc caaaccaact
     1921 tttaatttct ctgtcctcgt cggttagtag tggtctcagt tggcgttctt ttattgttgt
     1981 gcagagtctg gatgccccag ttgatattgt ctttgttctc aaccaatagc ttattgaggg
     2041 cccgttggac gatcatcatg cgattatcat tgttggcact tggggtatcg ttattccatg
     2101 aattccattg catactgcca gagtcatcaa taaaaaacat cacattaggc ttcacgccac
     2161 ctacagaagt agtcgtggtt tcgtcttgca aatgcagtgg tgtggtgggg aaggtaccac
     2221 ctaatgatga ggtggtgaaa ccgcacagag tgagcaaata gaccaaacgt ttaagtggtg
     2281 tgtgtgcacc tgtcttgtat ttgtttaatg tattcataat gtctatgatc cagaataatt
     2341 ggtttttaaa tgccatttaa aactgtcgat aaatgaaaat ggcattcaac aaaataaagt
     2401 atttaattta agtgttgaag actgagttct aattaattta cttgatatat aaagaattag
     2461 tagtatttag cgcctaaata taaacactaa tttatgcaaa ttgaattatg agaataatag
     2521 cattcatgaa gcaggaacgt gatgattgga gtcaatggaa acttttttca tctcaaaggt
     2581 tgtttagtat ttagactttt attatgaata tggacattaa aatagtatga tgaaatttga
     2641 tgcgaacaat cacaaaagaa gtgagctcaa ttactcacag catgactgat tgaagaaggg
     2701 gagttttgtg gtgagaacaa agcttggagt ctgaataggc ggcctgtaaa gctgacagac
     2761 cgcttgaaat gaacttgaga agaataagta ggggtgaaaa atcagcgatt atcgattttt
     2821 tctgggtaca tgtcgtgact aaacaggcgg tgttcggcca tttgggtgta tttgctgtcg
     2881 gggcggccgt aattgcaata agggtcgatg gagaggccgc cgcgcggggt gaatttgccc
     2941 cacacttcaa tgtagcgcgg attcaacaag gagattaagt cgagcatgat tttgttgatg
     3001 cagtcttcgt gaaaatcacc gtggttgcgg aaactgaaca aatacagttt taaggatttg
     3061 ctttcaacca atttttcatc ggggatgtaa gagatgtaaa tggtggcgaa atcgggctgg
     3121 cctgttaagg ggcacaggct ggtgaattcg gggcagttga atttgacgaa atagtcgcgt
     3181 gctgtgtgca aattgggcac ggcttcgagc acgctgggct ggtattcgct ggggtattgg
     3241 gtgttttgat tgcctaatag ggttaagccg tcgagttctt gggcgtttct gctgctcatg
     3301 tgtttgcttt catgtgtaca cggcacgcca cagtctatgg ttttttaaag agggtgggtg
     3361 ccataaacct ctgggggtag gcattataac cgataagtct ggctggctgt gtcagacgat
     3421 gtgcgcaggt ggtttacagg ccgcttgtga tgcaaacaca gggttaagca aaagtgagca
     3481 gacatgagtc gtgaacaaca atggttggaa tgggcggcaa aattggattt tgatacccat
     3541 gccgccatta aaattggtgg caattatgtg gcggtgttgc gcgatggcgc gcaagtgtat
     3601 gtgagtgggc aaattccacg aatattggac actgtggtgg taacaggccg tgcgggtgag
     3661 gatgtcactt tggttcaggc gcaatatgcg gcgcgggtgt gtgccatgcg ggctttaagt
     3721 ttgttgagac aagagttggg cagcttggat gcggtgcaac aggttttgcg catcaatgtg
     3781 tatgtgcaaa gtgcgacaga ctttactttg caaagcgaag tggcggatgc agcttcaggt
     3841 gtgttgtttg aggtgttggg cgcagcgggc aggcacacgc gcacgtctgt cggtgtgtac
     3901 caattgccga aaaatgccac ggtcgaattg gatttggtgg ccacagtggc ggcttaatgg
     3961 ttgcttatgc catggttgtt gaaacttatc cacatattgc cattgcgagt gtctgtggat
     4021 gatttacatt atagagaaag ataaaatgcg gcctgtaaat gctttgagcg atttacaggc
     4081 cgcttgtttt gacttatcca gaattgatgc gccacttatt caagacttat acaaaactta
     4141 tccacatcag gcaggttcgg tttcgtcttc ggcgctgtgg atgaatttat tgaaattcag
     4201 gctgatggtt tgcaaaggca gctgtatgcc tgtgacattg aaccaagcgc gtgagcgcgg
     4261 catgcacacg ggcacgccca cggcgcggct gaccaatggc aagccatcga tgcgcaccaa
     4321 atcttctttc aagaccaccg ctttcaaatg gcgcaagcct tgttgctgga tgtaaaccaa
     4381 gctccaatag ctttccattt tgcgttgaaa atcggcgtat gcgctgtaag tggtgtcaaa
     4441 atcacgcatt aaggcaaaca catcgctcga ttgcatgtca aaacgcggag tgaaatgttc
     4501 aggctcaatc aggctgagca attggcgttg gttgaagtaa tcggtggcgc ggcgcagtgg
     4561 cgatgtgcac catgcataat gctcaacacc catgccgatg tgcgcttctg attgggtgct
     4621 catgcgcact ttgcctgcgg gctgaacgcg gtaaatggca gcgcgttcgc cttcgtgcag
     4681 catttttgcc caagtcgagt tggccaaaat catcatttct gacaccaaag tgtcaatcgg
     4741 cgaaccgcgt tcgcgcttgg caatgctcac ttggccgtct tcgctcaaat cgatgctgta
     4801 atcgtattgg
//
LOCUS       DAOMVK010000133         4787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_5032_length_4787_cov_3.178360, whole genome shotgun sequence.
ACCESSION   DAOMVK010000133 DAOMVK010000000
VERSION     DAOMVK010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4787
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5032_length_4787_cov_3.178360"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1477)
                     /gene="thrS"
                     /locus_tag="PK856_05680"
     CDS             complement(<1..1477)
                     /gene="thrS"
                     /locus_tag="PK856_05680"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRL01895.1"
                     /translation="MPQITLPDGSIRSFDHPVSVMEVAQSIGTGLAKATIAGKVNGRL
                     VDASDLITEDAALNIITAKDQEGVEIIRHSCAHLVGHAVKQLYPDVQMVIGPVIEDGF
                     YYDIFSDKPFTPEDMEAIEVRMHQLIDQDYDVIKKMTPRAETIKTFTARGETYKLRLI
                     DDMPDETEMGLYYHQEYVDMCRGPHVPNTRFLKAFKLTKLSGAYWRGDSKNEQLQRIY
                     GTAWADKKQLKEYIQRIEEAEKRDHRKLGKQLDLFHMQDEAPGMAFWHPKGWALWQVI
                     EQHMRTELNAAGYKEIRTPMVMDKILWEKSGHWENYKENMFITESEKRDYAVKPMNCP
                     GHVQVFNQGLRSYRDLPLRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     DSEAQAFNRLVMKVYKQFEFNDVSVKLSLRPEKRAGSDETWDRAEEGLRNALRACDVE
                     WEELPGEGAFYGPKVEYHIKDALGRSWQCGTIQLDFVLPERL"
     gene            complement(1577..1653)
                     /locus_tag="PK856_05685"
     tRNA            complement(1577..1653)
                     /locus_tag="PK856_05685"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1617..1619),aa:Val,seq:gac)
     gene            1765..>2532
                     /gene="tgt"
                     /locus_tag="PK856_05690"
     CDS             1765..>2532
                     /gene="tgt"
                     /locus_tag="PK856_05690"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRL01896.1"
                     /translation="MLKFELLKQDGLARRGRITLNHGTVETPVFQPVGTYGSVKAMTP
                     KDLDDINAQIILGNTYHLWLRPGLEVIKEFGGLHDFMNWHKPILTDSGGFQVFSLSNM
                     RKLTEEGCTFKSPINGDKLFLSPEISMQIQTVLNSDIVMQLDECTPGDATAHQAQKSL
                     QMSLRWAERSKNEFERLNNPNALFGIVQGAMFEDLRDESLSGLNALDFDGVAIGGLSV
                     GESKEDMYRILSYIGPKLPANKPHYLMGVGTPEDLVEG"
     assembly_gap    2533..2542
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2543..2876
                     /locus_tag="PK856_05695"
     CDS             <2543..2876
                     /locus_tag="PK856_05695"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004286160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HRL01897.1"
                     /translation="MPTRNARNGWLFTRYGDLKIKNSAHKYDKRPLDESCTCYTCRNF
                     SRAYLHHLHRVGEILGAQLNTIHNLHYYQQLMQDVRDAIDAGEFANFRIRFAAERARG
                     VQSRCKTD"
     gene            2953..3246
                     /gene="yajC"
                     /locus_tag="PK856_05700"
     CDS             2953..3246
                     /gene="yajC"
                     /locus_tag="PK856_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRL01898.1"
                     /translation="MNEQVMSFLPMILVLAVFYFLVMRPQQKKMKAHQNMLAELKKGE
                     KVITSSGMVGKITKVSERFFTIEIAPNVQVDFERSAVTGRIDASGAAIEEKKD"
     gene            3308..>4787
                     /gene="secD"
                     /locus_tag="PK856_05705"
     CDS             3308..>4787
                     /gene="secD"
                     /locus_tag="PK856_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HRL01899.1"
                     /translation="MNRYPLWKYLLIVVLVVLSCLYTIPNFYGETPAVQISTNRQAIQ
                     INDALAQKINSALTQAKINTDGQFFDSGSLKIRFHTEEDQIKARDVIDNSLGEGYIIA
                     LNLIPNTPVWMSQIGARPMFLGLDLRGGVHFTMQVDMEAAKQKTLERIAGDIRREAKK
                     QKFTISDAKITADKVIVPFQNAADAQAIVRSMSKQLGTEANINLVAGNTVEASLSEAQ
                     LLSISSSAVAQNMNTLHNRVNELGVAEPVIQQAGTNRIVVQLPGVQDTAKAKDILGRT
                     ATLEVRMVSDDAALIQQAMLGTVPEGYELLSNSGGQGSTLVSKQVELTGDNINDAQPG
                     FTENNQPSVNLVLDSAGSDIFADLTRANRGKRMAMVLKDQGKSEVVTAPNINEPITGG
                     RVQISGSMTAAEANDTALLLRAGSLAAPMKIIEERTIGPSLGAENIQKGFHSTLWGFV
                     AVACFMILYYRMFGVFSAISLTFNLLFLIAILSILQATLTLPG"
BASE COUNT         1285 a         1187 c         1072 g         1233 t
ORIGIN      
        1 ccaaacgctc tggcaacaca aagtcgagct gtatcgtacc acattgccaa gaacgaccca
       61 aagcgtcttt aatgtgatac tccactttcg gaccatagaa agcgccctca cctggcaact
      121 cttcccattc aacatcacag gcacgcaagg cattgcgtaa accctcttct gctctgtccc
      181 aagtttcatc agagcccgca cgtttttcag gccgtaatga cagcttcaca gacacgtcat
      241 tgaattcaaa ttgcttgtaa actttcatca ccaaacggtt aaacgcttgg gcttcagaat
      301 cgatttggtc ttcggtacag aaaatgtgcg catcatcttg cacgaaacca cgcacacgca
      361 tcaagccatg caaagcgcca gatggctcat tgcggtgaca agaaccaaac tcagccaaac
      421 gcaaaggcaa atcgcgataa gagcgcaaac cttgattgaa cacctgcaca tggcctgggc
      481 agttcattgg ttttaccgcg taatcacgtt tttcagactc ggtaatgaac atgttctctt
      541 tgtagttttc ccagtggcct gatttttccc acaagatttt atccatcacc ataggtgtgc
      601 ggatttcttt gtagccagct gcattcaatt cggtgcgcat gtgctgctct atcacttgcc
      661 acaatgccca acctttagga tgccaaaacg ccataccagg ggcttcgtct tgcatgtgga
      721 acaaatccaa ttgcttgccc aatttgcgat ggtcgcgctt ttcagcttct tcaatgcgct
      781 gaatgtattc tttcaattgc tttttatcgg cccaagcggt gccgtaaatg cgttgcaatt
      841 gttcgttttt agaatcgcca cgccagtatg caccagacaa cttggtcaat ttaaacgctt
      901 ttaagaaacg ggtattgggc acgtgagggc cacggcacat gtccacgtat tcttgatggt
      961 aatacaagcc catttcggtt tcatcaggca tgtcgtctat cagacgcaat ttgtaggttt
     1021 caccacgcgc agtaaaggtt ttgatggttt cagcacgcgg cgtcatcttt ttaatcacat
     1081 cgtaatcttg gtcgattaac tgatgcatgc gcacttcaat cgcttccatg tcttctgggg
     1141 taaatggctt gtcactgaag atgtcgtagt aaaaaccatc ttcaatcaca ggtccaatca
     1201 ccatttgcac atcagggtac aactgtttga ctgcatgacc caccaaatgc gcgcaagagt
     1261 ggcgaatgat ttccacaccc tcttgatctt tggcagtgat gatgtttaag gccgcatctt
     1321 ctgtaatcaa atcgctggca tccactaagc gaccatttac cttgcccgca atcgtggctt
     1381 tggccaaacc agtacctatt gattgcgcca cttccatgac acttacagga tggtcgaacg
     1441 atctgataga accgtccggt aaagtaattt gaggcatgat tacactccgt gctgctttat
     1501 tattcagtgc cgcaagaggc ataacaccgc ttgctggcga aaaaacagcg aacattgggt
     1561 tcgctaattg ataacttggt aggcatgatt ggattcgaac caacgacccc caccatgtca
     1621 aggtgatgct ctaaccaact gagctacatg cctatgctta tatcgcaaag aaaacgataa
     1681 ttgtagcgac tatcatatta attggcaaca cacagaacgc agtgattcgc ttataatagc
     1741 ggttttgttt gcgtgcaaac ccttatgctt aaatttgaac ttttaaaaca agatggtctt
     1801 gctcgtcgcg gtcgcatcac cttaaatcat ggcactgttg aaacccctgt cttccaacca
     1861 gtaggcactt atggcagtgt caaagcgatg acacccaaag acttggacga catcaatgcc
     1921 caaatcattc tgggcaacac ctaccatttg tggctgcgcc ctggtcttga agtcatcaaa
     1981 gagtttggtg gtttgcacga tttcatgaat tggcacaaac ccatactcac cgactcaggt
     2041 ggctttcaag tgtttagctt gtctaatatg cgcaagctca ctgaagaagg ctgtaccttc
     2101 aaaagcccga tcaatggcga taaattgttt ttgtcgcccg aaatttccat gcaaattcaa
     2161 accgtgctca attcagacat tgtaatgcaa ttggatgaat gtacgcctgg tgacgccacc
     2221 gcccaccaag cgcaaaaatc cttgcaaatg agtttgcgtt gggccgagcg ctctaaaaat
     2281 gaatttgagc gtttgaacaa ccccaatgcc ttgtttggca ttgtgcaagg cgcgatgttt
     2341 gaagacttgc gcgatgagtc tttaagcggc ctgaatgcct tagattttga tggtgtggcc
     2401 atcggcggct tgtctgtcgg cgaatccaaa gaagacatgt accgcattct gagctacatc
     2461 ggcccgaaat tgcctgccaa caaaccacat tacttgatgg gcgtgggtac tccagaagac
     2521 ttggtagaag gtnnnnnnnn nngatgccaa cgcgcaatgc gcgcaatggt tggttattta
     2581 cccgctatgg cgacttgaaa atcaagaact ctgcacacaa atacgacaag cggcctttag
     2641 acgaaagctg cacttgctat acctgtcgca atttcagccg tgcctattta caccatttac
     2701 accgcgtggg cgaaatcttg ggtgcgcaat tgaacaccat ccacaatttg cattattacc
     2761 aacaattgat gcaagacgtg cgcgatgcca ttgatgctgg cgagtttgcc aacttccgca
     2821 tacgctttgc agcagagcgt gctcgcgggg tgcaaagtcg ttgtaaaact gactgaggtc
     2881 ttttgacatt tacccaagag gctataaaat agccgattac tcaattcaac tagaaataca
     2941 ttaaggatca taatgaacga acaagtcatg tcgtttttgc ccatgatttt ggtgctggcc
     3001 gtgttttact tcttggtaat gcgcccacaa caaaagaaaa tgaaagccca tcaaaacatg
     3061 ttggctgagc tgaaaaaagg tgagaaagtc atcaccagtt caggcatggt cggtaaaatc
     3121 accaaagttt cagaacgctt ttttaccatc gaaatcgctc ctaatgttca agttgatttt
     3181 gaacgctcag cagttacagg ccgcattgat gccagtggcg ctgccatcga agaaaaaaaa
     3241 gactaaatta atccagcaac caaccgctcc tccatgggcg gttggttgtg tttcaaaggt
     3301 ctagggaatg aatcgctatc ctttatggaa atacttgttg attgttgtgt tggtggtact
     3361 gagctgtctg tacaccatcc ccaacttcta tggggaaacc cctgcagtac aaatttccac
     3421 caatcgccaa gccatccaaa tcaacgatgc cttggctcaa aaaatcaaca gtgcgttaac
     3481 gcaagccaaa atcaataccg atggtcaatt ctttgacagc ggcagtttga agattcgctt
     3541 tcataccgaa gaggatcaaa tcaaagcccg tgatgtgatt gacaactcat tgggcgaagg
     3601 ctacatcatc gccttgaatt tgattccaaa cacccctgtg tggatgagcc aaatcggtgc
     3661 tcgccccatg ttcttgggtc tggacttgcg tggtggcgtg catttcacca tgcaagtaga
     3721 catggaagcc gccaagcaaa aaaccttaga gcgcattgct ggcgacattc gccgcgaagc
     3781 caagaaacaa aaattcacca tcagcgatgc caaaatcaca gctgataaag tgattgtgcc
     3841 tttccaaaat gctgccgacg cgcaagcaat cgtgcgcagc atgagcaaac aattgggcac
     3901 cgaagccaac attaacttgg tggcaggcaa cacagtagaa gcctctttgt ctgaagcgca
     3961 attgttgtcc atcagctcgt ctgcggtggc acaaaacatg aacaccttgc acaaccgtgt
     4021 caatgaattg ggtgtggctg aacctgtgat tcaacaagca ggcaccaacc gcattgtggt
     4081 gcaattgcct ggtgtgcaag acacagccaa agccaaagac attttgggtc gtaccgccac
     4141 tttggaagtg cgcatggtgt ctgatgatgc tgcattgatc caacaagcca tgttgggcac
     4201 agtgcctgaa ggctatgaat tgttgtccaa ttctggcggg caaggctcaa ccttggtcag
     4261 caagcaagtg gaattgaccg gtgacaacat caacgatgcc caacctggct ttaccgaaaa
     4321 caaccaacct tcggtgaatt tggtactgga cagcgcaggc tcagacattt ttgctgacct
     4381 gactcgcgcc aaccgtggca aacgcatggc catggtgtta aaagaccaag gcaaatcaga
     4441 agtggtgacc gcacccaaca tcaatgaacc gattacaggt ggccgtgtac aaatttcagg
     4501 cagcatgacc gcagccgaag ccaacgatac cgcattgtta ttgcgtgcgg gctctctggc
     4561 ggcaccgatg aaaatcattg aagagcgcac cataggcccg agccttggtg ctgaaaacat
     4621 ccaaaaaggc ttccactcta ccctgtgggg ttttgtggcc gtggcttgct tcatgatttt
     4681 gtattaccgc atgtttggtg tgttctcggc gatttccttg accttcaact tgttgttctt
     4741 gattgccatc ttgtcgattt tacaggctac cttaacctta ccaggta
//
LOCUS       DAOMVK010000134         4774 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_5053_length_4774_cov_3.266582, whole genome shotgun sequence.
ACCESSION   DAOMVK010000134 DAOMVK010000000
VERSION     DAOMVK010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5053_length_4774_cov_3.266582"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            244..1704
                     /gene="gabD"
                     /locus_tag="PK856_05710"
     CDS             244..1704
                     /gene="gabD"
                     /locus_tag="PK856_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HRL01900.1"
                     /translation="MMQLQDSALFKTQCYIDGVWLDADNKATVEVTNPANGEVIGTVP
                     QMGKAEADRAVAAAQAALPAWKAKSAKERSQILRKWFDLMMAHQEDLGKILTLEQGKP
                     LAEAKGEIAYGASYIEWYAEEGKRIYGDIIPSTGLDKRILVTKQPIGVCAAITPWNFP
                     NAMITRKAAPALAAGCTFVIRPASQTPFSALAIAELAERAGIPKGVFNVLTGSSSQIG
                     EVLTKDDRVKKFSFTGSTEVGRKLIEQCASTVKKVSMELGGNAPFIVFNDADLDAAVE
                     GAMICKFRNAGQTCVCANRLYVQSGVYDQFVEKFKAAVAKLSIGNGMDAGVNFGPVID
                     ANAVKKVEEHIADATSKGATVVAGGQPHALGGLFFEPTIVTGATQEMQFAKDETFGPL
                     APVFKFETEEEVLGYANDTEFGLASYFYTRDIGRVIRVSEGLDYGMVALNTGILSNEA
                     APFGGVKQSGLGREGSKYGIEDYVEIKYVLLAGLDK"
     gene            complement(1771..2682)
                     /locus_tag="PK856_05715"
     CDS             complement(1771..2682)
                     /locus_tag="PK856_05715"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRL01901.1"
                     /translation="MNTTDFQFFVRVAALGSISQAAKEAHLSVSVASQKIQRLEQDLQ
                     LRLFHRTTRKLHLTTEGECLLKQGQPLLAEFWQLHEGLSQGKQELTGRLNITAPSSFG
                     NHVLIHALADFQKQHPQLQLCIDLNDQNVDLVATGMDAAIRIGILKDSSLIAKPLSAN
                     PRLLCAAPEYLQQYGEPLSPADLIQHRCLLQQHSHGITNKWSLFNAKNAIESVSVQGY
                     FVSNSGEAIRQACLHGMGIGNFSLWHVADDLAQGRLQAVLTAHTIESSTIYLLYPARQ
                     LLPLKVQLLSEFLQEHFQHHYKFGLNG"
     gene            2795..3844
                     /locus_tag="PK856_05720"
     CDS             2795..3844
                     /locus_tag="PK856_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRL01902.1"
                     /translation="MKYNYLGNSGLRVSELGFGAGTLGGKGPLFSAWGQASQNEADNM
                     IAACVDAGVNFFDTADVYSEGDSERMLGKALVPYRQQSIVSTKVSLRLDEGINQVGFS
                     RKYLLDAVEASLKRLQSDYIDVLQLHHFDSMTALLQLMATLDQLVRSGKVRYIGASNF
                     SGWQLMKAQAIAAQYGYESFVAHQVYYSLIGREYEWDLMPLAHDQNIGSVVWSPLGWG
                     RLTGKFDRQHPLPVQSRLHQTAQFAPPVAEEHLYAVIEGLQQVAAATGKSVPQVALRW
                     LLQRPTVATVLVGARNVSQLHDNLAAATWQLSAEHMALLNHVSAVYPIYPHYPYWNGQ
                     FSERHLSLVDSEFLA"
     gene            4184..>4774
                     /gene="recO"
                     /locus_tag="PK856_05725"
     CDS             4184..>4774
                     /gene="recO"
                     /locus_tag="PK856_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRL01903.1"
                     /translation="MSRRSTAKIRVDAQPIFILSNSPWRESSLWLEVFSRDHGRVALI
                     ARSARKRQSDLRGVLMPFVPVEVAWFGQQDNYTLHTATWRGGWAQPTGQELMSAWYVN
                     ELLLNLTAREDAMPRLYDAMVAVMRSLSHKEDTATALRQFEWQLLQNLGLAPDLSVDT
                     HEQDLDAQAQYWMLPEQAPFKVGSDARFSHVREAVKV"
BASE COUNT         1199 a         1054 c         1290 g         1231 t
ORIGIN      
        1 tcaccaaacg tgcccaaacc cacgccttgg aaaatggctt gattttgctc acttgtggca
       61 tgtattacaa cgtaatgcgc ttcttgttcc cagtgaccat cgaagacgaa atcttcgaag
      121 agtctttggg catcttagaa gccgccttaa cccacgcagc agcaactgca gaagcataat
      181 gcaacattaa ataaggcgtc ctgtaagctc agggcgcctt tattttgagg acatgaagga
      241 attatgatgc aattacaaga cagcgctttg ttcaaaaccc aatgttacat cgatggcgta
      301 tggttggatg ccgacaataa ggcaacagta gaagtcacca atcctgccaa tggcgaagtg
      361 atcggtaccg tgccacaaat gggcaaagcc gaagcagacc gtgccgtggc cgctgctcaa
      421 gccgcattgc cagcatggaa agcgaaaagc gcgaaagagc gcagccaaat tttgcgcaaa
      481 tggtttgatt tgatgatggc ccaccaagaa gacttgggca aaatcttgac cttggaacaa
      541 ggcaaaccat tggccgaagc caaaggtgaa atcgcgtacg gcgcttctta catcgaatgg
      601 tatgccgaag aaggcaaacg catttacggc gacatcattc catcaacggg cttggacaaa
      661 cgcattttgg tgaccaaaca gccaatcggt gtgtgcgctg ccatcacacc ttggaacttc
      721 ccaaatgcaa tgattacacg caaagccgca ccagctttgg ctgcaggctg tacttttgtg
      781 atacgcccag catcacaaac cccattctca gctttggcga ttgccgaatt ggctgagcgc
      841 gcaggcattc ctaaaggcgt gttcaacgtg ttaacaggca gctctagcca aatcggcgaa
      901 gtgttgacca aagacgaccg cgtgaaaaaa ttcagcttca caggctctac tgaagtgggt
      961 cgcaaattga ttgaacaatg tgcgtctacc gtgaaaaaag tgtctatgga attgggcgga
     1021 aacgcgccat tcatcgtgtt caacgatgcc gatttggacg cagcggttga aggcgcgatg
     1081 atttgcaaat tccgcaatgc gggtcaaact tgcgtgtgcg caaaccgttt gtatgtacaa
     1141 agcggcgtat acgaccaatt cgtagaaaaa ttcaaagccg cagtagcgaa attgtcgatc
     1201 ggtaatggca tggacgcagg cgtgaatttt ggcccagtga ttgatgccaa cgccgtgaaa
     1261 aaagtggaag aacacattgc tgacgccact tctaaaggcg ctaccgttgt ggcaggtggt
     1321 caacctcacg ctttgggcgg cttgttcttc gagcctacca tcgtgacagg cgctacccaa
     1381 gaaatgcaat ttgccaaaga cgaaaccttc ggcccattgg ctcctgtgtt caagtttgaa
     1441 accgaagaag aagtgttggg ctatgccaac gacaccgaat tcggcttggc ttcttacttc
     1501 tacacccgtg acattggccg cgtgattcgc gtgtctgaag gcttggatta cggcatggtg
     1561 gccttgaaca caggcatctt gtctaacgaa gctgccccat tcggtggcgt gaaacaatct
     1621 ggcttgggcc gcgaaggttc taaatacggc atcgaagact atgtggaaat caaatacgtg
     1681 ttgttggcag gtttggacaa ataagcctta aaacacttgt attgagatga aaagcggcct
     1741 gtaaatgttt acaggccgct tgtttttatt ttagccatta agaccaaact tgtaatggtg
     1801 ttggaaatgc tcttgtaaaa actcagacag caattgtact ttcagtggca gcagctgacg
     1861 ggctggatag agcaggtaga tggtgctgga ttcaatggtg tgggcggtta aaacagcttg
     1921 caatctgcct tgagccaaat catcggcaac gtgccacaaa gagaaattgc ctatgcccat
     1981 gccgtgtaag caggcttgtc ttatggcttc acctgagttg ctgacaaaat agccttgcac
     2041 cgaaacagac tcgatggcgt tttttgcatt gaacagcgac catttgttgg tgatgccatg
     2101 actgtgttgt tgtaacagac agcgatgctg tattaaatct gcagggctta gtggttctcc
     2161 atattgttgc agatattctg gggcagcgca cagcaaacga ggattggcac tcagtggctt
     2221 ggcaatcaag ctagaatcct tgaggatgcc aattcgtatg gcagcatcca tgcccgttgc
     2281 aaccaaatcc acattttggt cattcaaatc aatgcacaat tgcaattgtg gatgttgctt
     2341 ttgaaagtct gccaaggcat gtatcagcac atgattgccg aagctcgagg gtgctgtgat
     2401 gtttaagcgg cctgtaagct cctgcttgcc ttgggataag ccctcatgta gctgccaaaa
     2461 ctctgccagc aaaggttgcc cttgctttaa taagcattca ccttccgtgg taaggtgtaa
     2521 tttgcgggtg gtgcgatgaa acagtctgag ctgtaaatcc tgctctaagc gttgaatctt
     2581 ttgactggcg actgacacag ataggtgggc ttctttggct gcttggctga tggaacctag
     2641 cgcggctacc cgtacaaaaa actgaaagtc tgttgtgttc attatcgtga ttatccaaaa
     2701 agtcattccc gttttaacgg gtttttctat ggtgtgaaac gccgtaagct caccttacca
     2761 atttttgatt gagtgagaaa ggcaggatgg attcatgaaa tacaattact taggaaattc
     2821 gggtttgcgt gtgtctgagt tgggctttgg cgcgggcacg ttgggcggga aagggccgct
     2881 gttctctgca tggggtcaag ccagtcaaaa cgaagctgac aatatgattg ctgcctgtgt
     2941 ggatgcagga gtgaattttt ttgatacagc agacgtgtat tctgagggag attcggagcg
     3001 tatgctgggc aaggcattgg tgccgtaccg acaacaatcc atcgtatcga ccaaagtcag
     3061 tttgcgcctg gatgagggca tcaatcaagt ggggttttct cgtaaatacc tattggatgc
     3121 agtagaggct tcgcttaagc gtttgcaaag tgactacatt gatgtattgc aattgcacca
     3181 ttttgacagc atgacagcct tgctacaatt gatggcaacg ctggatcaat tggtgcgcag
     3241 tggtaaggta cgctatatcg gagcatcgaa tttttcgggc tggcagttga tgaaggcgca
     3301 agccattgct gcgcaatacg gttacgaatc ctttgtggcg caccaagtgt attattcttt
     3361 gataggtcgt gagtatgagt gggatttaat gccattggcg cacgaccaaa acatcggctc
     3421 ggtggtgtgg agtccgttgg ggtgggggcg cttaacgggt aaattcgacc gtcaacatcc
     3481 gttgcctgtt cagagccgct tacaccaaac ggctcaattt gcaccacccg tggcagaaga
     3541 gcatttgtac gcagtgattg aaggcttaca gcaagtggcc gcggctacgg gtaaaagtgt
     3601 gccacaagtg gcactacgct ggttgttaca gcggcctacg gtggcaacag tcttggtggg
     3661 ggcgcgcaat gtgtcgcagc tacatgacaa tttggctgct gccacatggc aactgagcgc
     3721 tgaacacatg gcgctgctga accatgtcag tgcggtgtac ccaatttacc ctcattatcc
     3781 gtattggaat ggacagtttt ccgagcgtca tttgtctttg gtggactctg agtttctagc
     3841 ataaataagc ggcctgtaag caacacatac aggccgctta tttatctgaa gtcttggtgt
     3901 caatgatgta gttggtgtta cagggcatac ctattgtgtt gattcaataa aatgcagtca
     3961 gtcataccat cactgcatgc agtctatttg tatgcagtgt ttccgtattg ggatacagac
     4021 cacaatgatt taaagtcata taaaaatgcg cgcagaaacc caattggcat tgtaaatttg
     4081 attaaatcca tctgtttggt tgagactgtg agctgtggag gcagcaggga tgggcggtct
     4141 ttgctataat ccatagcgat atgattacgg taagcttaca gtcttgagtc gtcgttctac
     4201 tgccaaaatt cgggttgatg cccaaccgat ttttattctc tccaacagcc catggcgtga
     4261 atccagtttg tggttggaag tgttcagccg cgatcatggc cgcgtggctt tgattgcgcg
     4321 cagcgcgcgc aagcgtcaaa gtgatttgcg cggggtgttg atgccgtttg tacctgtgga
     4381 ggtggcatgg tttggtcagc aagacaatta cactttgcac accgccactt ggcgcggcgg
     4441 ctgggcgcag ccaactgggc aagagttgat gagtgcttgg tatgtgaatg aattgctgct
     4501 caacctcacc gcacgtgagg atgcgatgcc tcgtttatac gatgcgatgg tggcggtgat
     4561 gcgttctttg agccataaag aggacacggc cacggctttg cgccagtttg agtggcagtt
     4621 gctgcaaaac ttgggtttgg cgcctgattt gagtgtggac acgcacgagc aagatttgga
     4681 tgcgcaggcg caatattgga tgttgcctga gcaagcgccg tttaaggtgg gttctgatgc
     4741 ccgatttagc catgtgcgcg aggcagtgaa agtc
//
LOCUS       DAOMVK010000135         4761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_5076_length_4761_cov_3.308542, whole genome shotgun sequence.
ACCESSION   DAOMVK010000135 DAOMVK010000000
VERSION     DAOMVK010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5076_length_4761_cov_3.308542"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..139)
                     /locus_tag="PK856_05730"
     CDS             complement(<1..139)
                     /locus_tag="PK856_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009118026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HRL01904.1"
                     /translation="MRVLITGARGQVGQSLRERAPEDWEVIFTDSKTLDITNSTTVDA
                     MI"
     gene            complement(127..681)
                     /gene="rfbC"
                     /locus_tag="PK856_05735"
     CDS             complement(127..681)
                     /gene="rfbC"
                     /locus_tag="PK856_05735"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HRL01905.1"
                     /translation="MQINQTFFDVVHLIDVPRFPDERGWFSESLHQQQYADLLGLSTP
                     AFVQSNVSHSHTHVLRGMHAQLTRPQGKLIQVLSGEIYDAFVDLRPKQASFGQWHGIR
                     LSAQKSQQLWIPAGFAHGFLTLAQDTTVLYHCSDYYVPNDQVTLLWNDPDINIQWPIS
                     HVNPVLSPKDQAGMTLKEYIKCAS"
     gene            complement(681..1550)
                     /gene="rfbA"
                     /locus_tag="PK856_05740"
     CDS             complement(681..1550)
                     /gene="rfbA"
                     /locus_tag="PK856_05740"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HRL01906.1"
                     /translation="MKGILLAGGKGSRLNPMTFAVNKHLLPVYDKPMIYYSMSILMLG
                     GIRDIALISNPEDQHLFQRLFGDGSRLGLNITYFVQEEARGIPDAFLITQDWLQDDDV
                     CLILGDNIFFGESFGHKLTSAINELNGATIFTQQVNNPQRFGVACFDEHDNITSLHEK
                     PKEFISNHAITGLYLFDNQVAKFAANTRPSGRGELEIMDVLEQYRQQQRLSHTTLGRG
                     FAWLDAGTAESLLEAGSCIHTIEKMQGLKIACLEELAVRQGWCDKATILQHQANQSQS
                     AYDLYVQKILEQM"
     gene            complement(1589..2587)
                     /gene="rfbB"
                     /locus_tag="PK856_05745"
     CDS             complement(1589..2587)
                     /gene="rfbB"
                     /locus_tag="PK856_05745"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: protein motif:HMM:TIGR01181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HRL01907.1"
                     /translation="MTKSIVVTGGAGFIGAHVVRNLTEVSYDDIVIIDKLSYAGHLGR
                     LSDVLVLPRVHFYQEDVCDTAKMSAILAKHQPWAIMHLAAESHVDRSIDAPTVFLHNN
                     IQGTQSLLQAALGYWQALPNDLQAAFRYLQVSTDEVFGDAEDNGYAAHEDSPYRPSSP
                     YSASKASADHWVQSYQRTFGLPTLISYCSNNYGPGQFPEKLIPLSIERIKNGETIPVY
                     GDGLQKRDWLYVVEHARALWLMLTKAEPNQCYVVAAGQPITNLQLLQALYLEVQKQLN
                     RPVNEVPIQFVTDRLGHDRCYSLDASKIKRELGWQEQTLLQDGLAATVHQALNAPL"
     gene            complement(2584..3714)
                     /gene="ribD"
                     /locus_tag="PK856_05750"
     CDS             complement(2584..3714)
                     /gene="ribD"
                     /locus_tag="PK856_05750"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HRL01908.1"
                     /translation="MSTEFTALQTAMMQRALHLAVCAGNTTSPNPKVGCVIAHGEQIV
                     GEGFHVQAGAPHAEVHALRQAGDLARGATAYVTLEPCSHTGKTPPCANALIEAGVAEV
                     ICAMVDPNPQVAGQGLARLQAASIHTAHGLLHEQAQAINRGFLSRIQRSRPFVTLKLA
                     ASLDGKTALADGTSQWITGSAARNDVHQERAARNAIITGSGTVLADNPQLNVRGVACL
                     KPPVRVVLDRSLRSPATAQIFDTTVAPTWLFTAAEHNTSAYSERGVRCFAWPEAHANN
                     SLTFVLHALAEAGINEAWVEAGAQLSAAFIQQNCVDELVLYQAPKILGEHAIGLLPLQ
                     ANAAVLAQESVWKTVSVQAIGNDIKWCLRHALNVGIHGEILV"
     gene            complement(3714..4292)
                     /locus_tag="PK856_05755"
     CDS             complement(3714..4292)
                     /locus_tag="PK856_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01909.1"
                     /translation="MKHLKLFGLSTLCLVLLAACNQSTESSVGDTPPAASAAPSTPAS
                     APVAASQTISSKDGKISVVVTGQYADKLSEASQLISDIPSEQLLFLQRDDSADVLLYA
                     ADLGKAKKDAATYLKALSDAVKADAALKNTNVGAVEGNKLAYQFSETLAETEVNQACL
                     AINEGNIYSVCAISSSQSPEQLAQLLSNTTVK"
     gene            complement(4308..4739)
                     /gene="nrdR"
                     /locus_tag="PK856_05760"
     CDS             complement(4308..4739)
                     /gene="nrdR"
                     /locus_tag="PK856_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HRL01910.1"
                     /translation="MDSRLINDSHSVRRRRRCMACDRRFNTYETAEIRMPQVIKSSGE
                     RVPFDENKLRTSMMRALHKRPINQDHVEEAILAIKQTIQQHGERDIPSQKVGELVMGQ
                     LSQMDEVAFVRFASVYHSFNDVSEFTQTIARLTQPKSNLAD"
BASE COUNT         1122 a         1128 c         1236 g         1275 t
ORIGIN      
        1 gaatcatggc gtctacggtg gtgctgttgg taatgtccaa agtttttgag tcagtaaaaa
       61 tgacctccca gtcttcggga gcgcgttcgc gcaaactttg acctacttga cctctcgccc
      121 ctgtgattaa gacgcgcatt ttatgtattc cttcaatgtc attccagcct gatctttcgg
      181 tgataacacc ggatttacgt gacttatggg ccactggata ttgatatctg ggtcgttcca
      241 caacaatgtc acttggtcgt tgggaacata gtaatcgctg caatggtaca acactgtagt
      301 gtcttgcgcc agtgttagaa acccatgcgc aaagccagca ggaatccaca attgctgact
      361 tttttgagca gaaagtctga taccgtgcca ttgaccaaat gacgcttgct taggacgcaa
      421 gtccacaaac gcatcgtaga tttctcctga taaaacttgg atgagtttgc cttgtggtcg
      481 agtcaattgg gcatgcatgc cgcgcaatac atgggtatga gaatgggaaa cattgctttg
      541 cacaaatgct ggtgtactta aaccaagtaa atcagcatat tgctgttggt gcagtgattc
      601 gctgaaccag ccgcgttcat caggaaagcg tggcacatca atcaagtgca cgacatcaaa
      661 aaatgtttga ttgatttgca tcacatttgc tccaaaattt tttgcacata caaatcgtaa
      721 gccgattggg attggttggc ttggtgttgc aaaatcgtgg ctttgtcaca ccagccttgg
      781 cgcaccgcca attcttctaa acaggcaatc ttcaagcctt gcattttttc gatggtgtgg
      841 atgcacgagc ctgcttcgag caagctttcg gctgtgcccg catccagcca tgcaaagccg
      901 cggccgaggg tggtgtggct caagcgctgt tgctggcggt attgttccaa cacatccatg
      961 atttccaatt caccacggcc agaagggcgg gtgttggcgg caaatttagc cacttgattg
     1021 tcaaacagat acaggccggt aatggcgtga ttggaaatga attctttggg cttttcatgc
     1081 aaagaggtga tgttgtcatg ctcatcaaaa cacgccacgc caaaacgctg tgggttgttc
     1141 acttgctggg tgaagatggt ggcaccgttg agctcgttaa tggcagaagt gagtttgtgg
     1201 ccaaagcttt caccaaagaa aatattgtcg cccaaaatca agcacacatc atcgtcttgt
     1261 aaccagtctt gggtaatcaa aaacgcatcg ggtatgcctc gggcttcttc ttgcacaaaa
     1321 taggtgatgt ttagacctaa acgactgcca tcgccaaaca agcgctgaaa caggtgttgg
     1381 tcttcgggat tgctgattaa ggcgatgtca cggatgccgc ccagcatcag aatcgacatg
     1441 gagtagtaaa tcatcggttt gtcgtacaca ggcaataaat gcttgttcac ggcaaacgtc
     1501 atcggattca aacggctgcc tttgcctcct gccagtaaaa tgcctttcat gacttccctt
     1561 tatttgaatt ttgttatttt tcggtgattt aaagcggtgc gttcaaggct tggtgtacgg
     1621 tggcggccaa accgtcttgt aacaaggttt gttcttgcca gcctagctca cgtttgattt
     1681 tgctggcatc aagagaatag cagcggtcgt ggcccagacg gtctgtcaca aattgaatcg
     1741 gcacttcgtt tacaggccgg tttaattgtt tttgaacctc aaggtataag gcttggagca
     1801 attgcaaatt ggtgatgggt tggcctgctg caaccacata gcactggtta ggctcagcct
     1861 tggtgagcat caaccacaag gcgcgcgcat gttccaccac atataaccaa tcccgttttt
     1921 gcaaaccatc gccatacaca ggaatggttt caccattttt gatgcgttca atagacagcg
     1981 gaatcagttt ttcggggaat tggcctggac cataattgtt gctgcaatag ctgattaaag
     2041 tgggcaagcc aaacgtgcgt tggtaagact gtacccaatg gtcggctgag gctttgctgg
     2101 cagaataagg gctgcttggg cgataagggc tgtcttcgtg tgccgcatag ccattgtctt
     2161 cggcatcgcc aaacacttca tccgtagaca cttgcaggta gcgaaatgcg gcctgtaaat
     2221 cgttaggcaa ggcttgccaa tagcccaatg cggcttgtaa caaggattgt gtgccttgga
     2281 tgttgttgtg caaaaacaca gtgggtgcat caatagagcg gtctacatgg ctttctgctg
     2341 ccaaatgcat gatggcccaa ggttggtgtt ttgccaaaat agcgctcatt ttagcggtgt
     2401 cgcacacgtc ttcttggtag aaatgcactc gaggcagcac caatacatcg ctcaaacgac
     2461 ccaagtggcc cgcatagctg agcttgtcta tgatgacgat gtcgtcataa ctcacttcgg
     2521 tcaaattgcg caccacatgc gcgcctatga aacccgcgcc accggtaacc acgatgcttt
     2581 ttgtcatacc aagatttccc catggatgcc cacatttaag gcatggcgta agcaccattt
     2641 gatgtcattg ccaatggctt gaactgaaac ggttttccaa acagattcct gcgctaaaac
     2701 agcggcattg gcctgtaatg gcagcaagcc aatggcgtgc tctcccagaa ttttgggtgc
     2761 ttggtacaac accaattcgt ccacgcagtt ttgttggatg aatgcggcgc tgagttgggc
     2821 acccgcttca acccaagctt cattgatgcc tgcttcagcc aaagcatgca atacaaaagt
     2881 taagctgttg ttggcatgcg cctctggcca tgcaaaacaa cgtacaccgc gttcagaata
     2941 agcgctggtg ttatgttcgg ctgccgtaaa cagccaagta ggtgccactg ttgtgtcaaa
     3001 gatttgtgct gtagctgggc tgcgcaagct gcggtctaat accacgcgca cgggcggttt
     3061 caaacatgcc accccacgca cattcaattg tggattgtcc gccaatacgg tgccgctgcc
     3121 tgtgatgatg gcattgcggg cggcgcgttc ttggtgcaca tcgttacggg ccgctgagcc
     3181 tgtaatccac tggcttgtac catcggccaa ggcggttttg ccgtccaaac tggcggcaag
     3241 ttttaaggtg acaaaagggc ggctgcgttg aatgcgtgat aaaaagccgc gattgatggc
     3301 ttgtgcttgc tcatgtaata agccgtgtgc ggtgtggata gaagcggcct gtaaacgtgc
     3361 caagccttgt cctgcaactt gaggattggg gtctaccatg gcacaaatga cttcagccac
     3421 gcctgcttca atcaaggcat tggcgcaagg tggggtttta cctgtgtggc tgcaaggttc
     3481 taaggtcacg taagcagtgg cgccgcgagc caaatcgcca gcttgacgca aggcatgtac
     3541 ttcggcatgc ggtgcgcctg cttgtacatg aaagccttcg ccaacaattt gctcaccatg
     3601 cgcaatgacg caaccgactt tggggttggg gctggtggtg ttaccagcgc agaccgccaa
     3661 atgcaacgcg cgctgcatca tggcggtttg tagtgcagta aattcagtcg acattatttg
     3721 accgtggtgt tgctcaacaa ttgtgccaat tgttctgggc tttggctgct gctgatggcg
     3781 caaaccgagt aaatattgcc ttcattgatg gccaagcagg cttgattgac ttcggtttca
     3841 gccaaggttt cgctgaattg gtaagccaat ttgttgcctt caactgcgcc cacattggtg
     3901 ttcttcaaag cggcatcggc tttcactgca tcgctcaacg ctttcaaata agtagcagca
     3961 tcttttttgg ctttgcccaa gtcagctgca tacagcaaca catcggcact gtcatcgcgt
     4021 tgtaaaaaca gcaattgttc gctcggtatg tcgctgatca attggctggc ttcagacaat
     4081 ttatcggcat attgacccgt cacgacaacg ctgattttgc catctttgct gcttatggtt
     4141 tgtgatgccg caactggggc gctggcaggt gtgcttgggg cagcgctggc cgcaggcggt
     4201 gtgtcaccaa cagaagattc ggtactttgg ttgcaagcag ccaataaaac caaacatagc
     4261 gtgctgaggc caaacaattt taggtgcttc atttcatccc tttgaagtta atcggcaagg
     4321 ttcgatttgg gttgggttaa acgggcgatg gtttgagtga attcagacac atcgttaaaa
     4381 ctgtggtaca cagaagcaaa gcgcacaaaa gccacttcgt ccatttggct caattgaccc
     4441 atcaccaatt cgcccacttt ctggctgggg atgtcgcgct caccatgttg ttgtatggtt
     4501 tgtttgatgg ccaaaatggc ttcttcgaca tggtcttggt taatgggccg tttgtgtaag
     4561 gcgcgcatca tgctggtgcg caatttgttt tcgtcaaaag gcacgcgttc accgctggat
     4621 ttaatgactt ggggcatgcg tatttcagcc gtttcgtagg tgttgaaacg gcgatcacat
     4681 gccatgcaac ggcggcggcg gcgcacgctg tggctgtcgt taatcaaacg cgagtctatg
     4741 acttgggttt ctaaatgatg g
//
LOCUS       DAOMVK010000136         4725 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_5118_length_4725_cov_3.630621, whole genome shotgun sequence.
ACCESSION   DAOMVK010000136 DAOMVK010000000
VERSION     DAOMVK010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4725
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5118_length_4725_cov_3.630621"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..786)
                     /locus_tag="PK856_05765"
     CDS             complement(196..786)
                     /locus_tag="PK856_05765"
                     /inference="COORDINATES: protein motif:HMM:NF012273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRL01911.1"
                     /translation="MLPGACSIVGTVALEWAGADYELVRMDHAGTKSPEFLAKNPQGS
                     VPLLEDGDFYLTQNTAIMQYLSEQYPQAHLFGQGDAKQQAKAHQWFGMVNADIHSKFS
                     IIFGPQGYAQSEAAQAEVVASAKGLLQGLFAAVDTHLNGRDYLAEGVSVADVYLLVTL
                     NWAAKTGVDLSACKNLEAYKARVSANPAVAKVLALQ"
     gene            complement(816..>2369)
                     /locus_tag="PK856_05770"
     CDS             complement(816..>2369)
                     /locus_tag="PK856_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3458 domain-containing protein"
                     /protein_id="HRL01912.1"
                     /translation="SYTEMNNFYTLTVYEKGAEIVRMLHTLLGEEGFQKGMKLYFQRH
                     DGQAVTCDDFRLAMADANGYDLSQFALWYSQAGTPQVRLRSDYDAETQTLHLQAAQTL
                     PMTPDTTQAKQPLMIPLKLSVLNQQGEVQDLGVNGQIQQETVLVLREAEQEWTLQGVS
                     DEPILSLLHGFSAPIYLDYPYTEAELTVLMLHEKDAFARWEAGQILYRRLMKQAVAAL
                     RDGTALPDSRSLTQALQQLMQQDIDAALLALMLTVPSEQEIWDQDFPQLDPTLLWQAR
                     EWLVDSIAKVCVNDLSALRHTMQERELNSGVENIADYHPLLAKYRSLTNTVRSYLPRA
                     QPESLTYFKQQYDKLAFNMTHEWGILQAINCSEQAIRDELLQQFARKFDEDALVMDKY
                     FMLVASSGRSDTFERVQAALSHPRFSMENPNKVRSLIGMFTRNIAEFHHESGRGYAFV
                     ADKIIALDGYNPQVAARLVQAFNVLSRVDDARQSLMLAQLERIQAQTNVSKDTAEIIG
                     KILLQHQVA"
     assembly_gap    2370..2379
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2380..3402)
                     /locus_tag="PK856_05775"
     CDS             complement(<2380..3402)
                     /locus_tag="PK856_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HRL01913.1"
                     /translation="MQTVHYLKDYKRPEFSITQLDLHFDIQNEYTQVKSRMVVLPKNS
                     SPTPLLLAGSAELVEVVLDGEILSRDAYQLDPAQETLTILSVPTESFVLEVTTQLYPN
                     KNKSLMGLYSSNDNLYTQCEPEGFRKITYYLDRPDVMTKFSTTIVADKKRYPVLLSNG
                     NQVATGDVDKKRHYVKWIDPFRKPSYLFALVAGDFACSKDEFVTQSGRKVAIHFYTSE
                     QDKHKVPYAIASLKRAMRWDEERFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKY
                     VFADAQSATDKDFEHIEAVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGD
                     MANRDVR"
     gene            3590..4356
                     /gene="xth"
                     /locus_tag="PK856_05780"
                     /pseudo
     CDS             3590..4356
                     /gene="xth"
                     /locus_tag="PK856_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448484.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
     gene            complement(4400..>4725)
                     /locus_tag="PK856_05785"
     CDS             complement(4400..>4725)
                     /locus_tag="PK856_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HRL01914.1"
                     /translation="NGSSADIEASAIISADGLTMAALLPQDVDEDRVGAMAAAMLALG
                     ERTARELNRGDLDQVMVKGNNGYILMSYAGHDAVLTVIAKEGAKLGLIFLDAKRAAKN
                     IVDIL"
BASE COUNT         1269 a         1101 c         1018 g         1327 t
ORIGIN      
        1 tgcctaaaac tgcgctgaat tttttatcag tgtcgtgaaa aaatggggct ggtttcgcta
       61 ccaatactgc catgttaaat ctcctcgttg aatggtgata gaactgaact tccaactgct
      121 tactgtaatt tgaatgtgct cattcggcaa attgaatttc ctcatgagca aattacaatt
      181 aactatgtgc ttggtttatt gcagtgccaa aaccttggcc accgcaggat tggccgacac
      241 acgggctttg tatgcttcca agtttttaca ggccgataaa tccacccctg ttttggctgc
      301 ccaattgagg gtaaccaaca aatacacatc ggccacagac acaccctcag ccaaatagtc
      361 gcggccgttt aaatgggtat cgacagcagc aaacaagcct tgcaacaaac ctttcgcact
      421 tgctaccact tcagcttgtg cagcctcgct ttgcgcgtag ccttgagggc caaaaatgat
      481 ggaaaatttg ctgtggatgt cggcattgac catgccaaac cactggtgag ccttggcttg
      541 ttgcttggca tcgccttgac caaacaaatg cgcttgcggg tattgctcag acaagtattg
      601 catgatggcg gtgttttgcg tcaggtaaaa atcaccgtct tccagcaaag gcactgagcc
      661 ttgcggattc ttcgccaaaa attctggcga cttggtacct gcatggtcca ttctgaccaa
      721 ttcatagtct gcaccagccc attccaatgc cacagtaccc acaatcgaac atgcgccggg
      781 taacatataa aatttcattg tttgcgtcct tatcgttatg ccacttggtg ttgcaacaaa
      841 attttgccga tgatttcagc cgtgtctttt gaaacatttg tttgcgcttg gatgcgctct
      901 aattgcgcca acatcaggct ttggcgggca tcatcgacac gactcaaaac attgaatgcc
      961 tgtaccaaac gagccgccac ttgagggtta tacccatcca aggcgatgat tttatccgcc
     1021 acgaatgcgt atccgcggcc actttcatgg tgaaattctg caatattacg ggtaaacatg
     1081 ccgattaaag agcgcacttt attcggattt tccatgctga aacggggatg gcttaaagcg
     1141 gcctgtacgc gctcgaacgt atcgctgcga ccgctgctgg ccaccagcat gaagtatttg
     1201 tccatcacca aagcatcttc atcaaattta cgcgcaaatt gttgcaacaa ttcgtcgcgt
     1261 atcgcttgct cggaacaatt gatggcctgc aaaatgcccc attcatgagt catgttaaaa
     1321 gccaacttgt catattgttg cttgaaataa gtcaggcttt ctggctgggc acggggtaaa
     1381 tacgagcgca cggtattggt gaggctgcgg tatttggcca acaatgggtg atagtctgca
     1441 atattctcca ccccactgtt caactcacgt tcttgcatcg tgtgacgcaa agcgctcaaa
     1501 tcattgacac agactttggc aatgctgtct accaaccact cacgcgcttg ccacaacaat
     1561 gttgggtcta gttgaggaaa gtcttggtcc caaatctctt gctcacttgg caccgtcaac
     1621 atcaaggcca acaatgccgc atcaatgtct tgctgcatca attgttgcaa ggcttgggtc
     1681 aatgaacggc tgtccggcaa tgccgtacca tctcgcaaag ccgcgactgc ttgcttcatc
     1741 aaacggcggt ataaaatttg gcctgcttcc cagcgcgcaa aagcatcttt ttcgtgcaac
     1801 atcaataccg tcaattcggc ttcagtgtat gggtagtcca aatagatggg cgcgctgaat
     1861 ccatgtagca aagacagaat gggctcatcg ctcacgcctt gtaaagtcca ttcttgttcg
     1921 gcttcacgca aaaccaatac ggtttcttgc tgaatttgac cattcacacc taaatcttgt
     1981 acctcgcctt gttggttcaa cacacttaat ttcaacggaa tcatcaaagg ttgcttggct
     2041 tgggtggtat caggcgtcat tggcaaggtt tgagcggcct gtaaatgtaa agtttgtgtt
     2101 tctgcatcat agtcactacg caaacgcact tgcggcgtac ctgcttggct gtaccacagc
     2161 gcaaactggc tcaagtcata accgttggca tcggccatgg ccaaacgaaa atcatcacaa
     2221 gtcaccgctt gaccatcatg gcgttggaaa tacaacttca tgcctttttg gaaaccttct
     2281 tcgcccaata atgtgtgcaa catgcgcaca atttctgcac ctttttcata cacagttaaa
     2341 gtgtagaagt tgttcatctc agtgtatgan nnnnnnnnng cgcacatcac gattggccat
     2401 gtcacccgaa aactcttgat cgcggaaaac agttaagcct tcttttaagg acaattgaaa
     2461 ccaatcgcga caggtaactc gattgcctgt ccaattatgg aaatactcgt ggccaatcac
     2521 cgcttcaatg tgctcaaagt ctttatcggt ggcactttgg gcatcggcaa acacgtattt
     2581 ggtgttgaaa atgttcaaac ctttgttttc cattgccccc atattgaagt cacccacagc
     2641 caccaccatg aagatgtcca aatcatattc caaaccaaac cgctcttcat cccaacgcat
     2701 ggcgcgcttc aacgaggcga tggcataagg gactttgtgc ttgtcttgct cagaagtgta
     2761 gaaatggatg gccactttgc gacctgattg agtgacaaat tcgtctttac tgcatgcaaa
     2821 atcacccgca accaaggcaa acaaataact gggcttgcga aatggatcga tccatttcac
     2881 ataatggcgt ttcttatcca cgtcgccagt tgccacttga ttgccattgg aaagcaacac
     2941 aggataacgc tttttgtctg ccacgatggt ggtggagaat ttcgtcatca catcagggcg
     3001 gtcgaggtag taggtaatct tgcggaagcc ttctggttca cattgggtgt acagattgtc
     3061 atttgatgaa tacaaaccca tcaggctttt gtttttgttc ggatacaact gtgttgttac
     3121 ttccaaaaca aaactctcgg ttggcactga aagtatggtc agagtctctt gggctggatc
     3181 caattgataa gcatcacggc tcagtatttc tccatctaac accacttcaa ccaactctgc
     3241 tgaacccgcc aatagcaaag gtgttgggct ggaattttta ggcaaaacca ccatacgtga
     3301 ctttacttga gtgtattcat tttgaatatc aaaatgcaaa tccaattggg taatactgaa
     3361 ttcaggacgc ttatagtctt ttaaataatg aacggtttgc atacagatgc tcctgttatt
     3421 cttatggctg ggtgtgcaat gcgggctatt gtacttccat ccacaggctt tgtcttaagc
     3481 tacatcatgc acactaagct tcttgcatag ttttacaagt ccatcaccag tcttgaagca
     3541 tgttcattga aaatgggcgc aatgattatt tacttggctc aggttgctta tgaaaatcac
     3601 ctcatggagt gtgaattcac ttaatgcgag attgtctcaa gttttgcaat ggttagaaac
     3661 caaacctgct ggtgttttgg ccttacgaga gttgaaactg gattaaggca aattttctat
     3721 ttcaggctta gtgcaagcag gccactacac ggtatggttt ggtcaaaaac ttataatgga
     3781 gtggctttgc tatttaagga tgaggcatct gatgtactta gaggaatgcc tgattataag
     3841 gatgaaccgg aacacataat tacggcaacc atcaacggaa tacgcatcat caatgtgtat
     3901 tccgccagtg gataagtgat tgacggctct aaattcctat acaagcaaac ttggtttttt
     3961 gccttacgcc aatttgtatc ctctcaaaaa cagcagtatg aaaaagtgat ggtgctggga
     4021 gattttaata ttgcccctgc agacacggat gtttatgacg agtataagtg gaacgataag
     4081 attttgtgtt cagtcgatga gcgttgttgg ttccaacata tttttgcttt agggctaagt
     4141 gacagtttta gaatgaaacg gtctgaagat gttcaatcac ctggtgggat tatcgtatga
     4201 gtagtatgtt caagcgcaaa ttgggcttat gcatagacca catcctaatt aagtgaaaaa
     4261 ttcaaaatta agatactggc tgttgatgtt gacgaaaacg ctcgatcttg ggaaagaccg
     4321 agcaatcatg cgcctattac tttagaagta gcgtgattga gtgttagaca tacttgccta
     4381 acactgccat acaaatttat tacaaaatgt cgacaatgtt tttggctgcg cgtttggcat
     4441 ccaaaaaaat caaaccaagc ttggcacctt ctttggcaat cacggttaac actgcatcat
     4501 gaccagcata actcatcaag atataaccat tattaccttt aaccattact tgatccaaat
     4561 cgccacgatt taattcacgt gcggtacgtt cacctaaggc caacatagct gcagccatcg
     4621 caccaacgcg gtcttcatct acgtcttgag gtaataaagc tgccatggtc aaaccatctg
     4681 cagagataat tgcagacgct tcgatgtctg ctgatgagcc attta
//
LOCUS       DAOMVK010000137         4711 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_5141_length_4711_cov_2.956615, whole genome shotgun sequence.
ACCESSION   DAOMVK010000137 DAOMVK010000000
VERSION     DAOMVK010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5141_length_4711_cov_2.956615"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PK856_05790"
     CDS             complement(<1..287)
                     /locus_tag="PK856_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRL01915.1"
                     /translation="MFWHQLALNVPLERLFTYSHPEVLPLGTRVVVTFRNKRHVAVVW
                     AHTDELDFDASKVLPILEVLLDTPPLSEDWRAMMAFCSRYYLYPIGATVFTA"
     gene            complement(304..1779)
                     /gene="cls"
                     /locus_tag="PK856_05795"
     CDS             complement(304..1779)
                     /gene="cls"
                     /locus_tag="PK856_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HRL01916.1"
                     /translation="MTAITTSEALVLVHTVLTLSCVLRVLYHQKNHGVALAWLGILFV
                     APVVGLIAYLIIGEPKLGRTREKRKQEIVAFYSEFVAPSFHDLPTDLGLSPKWQSLTQ
                     LAQNSTDFQALSGHQCQFLNDTDAMIAAMVADIEQAKRSVVLMFYIIDIKGEVEKLFL
                     TLEQAALRGVRCEILADGVGSAQFFSTSWHARLKKNGVLIHQSLSIGLMKSVWVRSDL
                     RNHRKLLVVDEQVAYTGSFNLVDPKMFKQGAGVGQWVDVMLRIQGPVVIQLLAMFYAD
                     VAVENKQNLTDIQAMWRERWSEAGGVSLQADGSHSVLQTIPSSPDQNHYVFYDTLICA
                     LHLAERDIVLTTPYFVPDEPLMLALTAAARRGISVTLIVPQKVDSFLVRYASQANYRV
                     LLQNGVQIALFTGGLLHTKSVVVDGEFALFGTVNMDMRSFYLNLELTMALYSPTEIHA
                     LALQQQAYLRDCVYIDAARWQQRSVWRTLLENTVRLMSPLL"
     gene            complement(1779..2576)
                     /locus_tag="PK856_05800"
     CDS             complement(1779..2576)
                     /locus_tag="PK856_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01917.1"
                     /translation="MIDFYEALGIPTNATSMDIEKAIRLARQRGDVDEKILSQARNYL
                     IDPKMRKVYDKKMGINAASRARPRSALKSDTPFWIPVLVIVIILVLGMVGMFYLRKDA
                     NEKRVEAEIRQSQEQTKQRERMTDGGNDGADVQARVNDWHQSQINVTHDEAHGAEYML
                     TTKKRLSESSVFKEMKLVLFLPQGDQAQLVMYADEPVSCSGECRIQLVFDADNRYSLP
                     AQAGNQYWITPISDSAVNAFRQDGTVNVTLPDTDHSQFQFTYKGLRQ"
     gene            complement(2789..3853)
                     /gene="hemE"
                     /locus_tag="PK856_05805"
     CDS             complement(2789..3853)
                     /gene="hemE"
                     /locus_tag="PK856_05805"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HRL01918.1"
                     /translation="MTELQNDVFLRALLKQDVDYTPVWMMRQAGRYLPEYRATRAQAG
                     DFLSLCKNTELATEVTIQPLERFDLDAAILFSDILTIPDAMGLGLYFAEGEGPKFERP
                     IRTEADVAALQVADMDKLRYVTDAVSSIRKALNGRVPLIGFSGSPFTLACYMIEGGGS
                     KEFRLIKTMMYQRPDLLHKILDVLADSVIVYLNAQIAAGAQAVQIFDTWGGALSHVAY
                     REFSLRYMQKIVAGLTREAEGRQVPVIAFTKGGGLWLEAMADIGVDALGLDWTVDLGA
                     ARGRVGDKVTLQGNFDPMALFGTPESIEQEVARILAEFGHGNGHVFNLGHGINQHTPP
                     EHAKVLIDAVHKLSKPYHAA"
     gene            4011..4442
                     /locus_tag="PK856_05810"
     CDS             4011..4442
                     /locus_tag="PK856_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01919.1"
                     /translation="MSESEQNPQNPVPTEAPAAVQATSAPSSLSKKSAGGTDLRQYTM
                     IIYILYMAAILVGITSIVGVIMAYVKRADFAGTEYEDHITYLIRTFWISLIGYLIGAV
                     LSIVGIGIIIIIAVGIWYIYRSIAGFIKFNDNKPINAMGWM"
     gene            complement(4524..>4711)
                     /locus_tag="PK856_05815"
     CDS             complement(4524..>4711)
                     /locus_tag="PK856_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HRL01920.1"
                     /translation="FKELGVRRKVPFFTMLIIVVAILLLFWEPSLVLFLFFLAYSLSG
                     YVMWGMQKLGMGKTKIA"
BASE COUNT         1254 a         1277 c         1061 g         1119 t
ORIGIN      
        1 gcggtgaaca ccgtcgcccc aatcggatac aaataatagc ggctgcaaaa cgccatcatc
       61 gcccgccagt cttcactcaa aggtggcgtg tctagcaata cctccaaaat cggcaacact
      121 ttgctggcgt caaaatccaa ttcatcggtg tgcgcccaca ccaccgccac atggcgtttg
      181 ttgcgaaacg tcaccaccac gcgcgtgccc aaaggtaaaa cttcaggatg ggaataggtg
      241 aacaagcgtt ctaagggcac gttcagtgcc aattggtgcc aaaacatttt ttacaggccg
      301 cctttataac aatgggctca ttaagcgcac ggtgttttcc aataaggtgc gccacaccga
      361 acgctgctgc caacgtgcgg catcgatgta cacacaatcg cgcaaatagg cctgctgttg
      421 caaagccaag gcatggattt cagtggggct gtacaacgcc atggtcaatt ccaaattcaa
      481 ataaaagctg cgcatgtcca tgttgacggt gccaaacaag gcaaactcgc catccaccac
      541 cacacttttg gtgtgcaaca agccgcctgt gaacaaggcg atttgcacac cgttttgcag
      601 cagcacacgg taattggctt gtgaggcata acgcaccaaa aaagaatcca ctttttgcgg
      661 cacaattaag gtcacgctta ttccacgtct ggccgctgcg gtcaatgcca acatcaaggg
      721 ctcatcgggc acaaaataag gggtggtcaa aacaatgtcg cgctcagcca aatgcagcgc
      781 gcaaatcaaa gtgtcgtaaa acacataatg gttttggtcg ggcgaggaag gaatggtttg
      841 caaaaccgaa tgtgaaccat cggcctgtaa gcttacgccg ccagcctcag accagcgttc
      901 acgccacatc gcttgaatat cggtcaaatt ttgtttgttt tcaaccgcca catcggcata
      961 aaacatcgcc agcaattgta tcaccacggg cccttgtatc cgcagcatca catccaccca
     1021 ctgccccaca cctgcacctt gcttaaacat ttttgggtca accaaattga aactgcctgt
     1081 gtacgcgacc tgttcatcca ccaccaaaag cttgcggtgg ttgcgcaaat cactgcgcac
     1141 ccacaccgac ttcatcaaac ctatgctcag agattggtgt atcaacaccc catttttctt
     1201 caaacgcgca tgccacgagg tggagaaaaa ctgcgcactg cccacaccat cggccaatat
     1261 ttcacaacgc acaccgcgca gagctgcctg ctccaaggtg agaaacaatt tctccacctc
     1321 acctttgatg tcgatgatgt aaaacatcaa caccacgctg cgcttggctt gctcgatgtc
     1381 agccaccatg gccgcaatca tcgcatcggt gtcattgaga aactggcatt ggtgcccact
     1441 gagcgcttga aaatccgtgc tgttttgcgc caactgggtt aaagactgcc acttgggcga
     1501 caagcccaag tcagtaggca aatcatgaaa actgggcgca acgaattcgc tgtaaaacgc
     1561 cacaatttct tgcttgcgtt tctcacgcgt gcgcccaagc ttgggctcgc caatgatgag
     1621 gtaggcaatg agcccgacca caggtgcaac aaacaaaatg cccaaccaag ccaaagccac
     1681 cccatgattc ttttggtggt agagcacacg caacacgcaa ctgagtgtca acacggtatg
     1741 caccaatacc aatgcctctg aggtggtgat ggcggtcatt attggcgtag tcccttatac
     1801 gtgaattgaa attggctgtg gtcggtatcg ggcaaggtca cgttaaccgt gccatcttgg
     1861 cgaaacgcat tgaccgcgct gtcactgatg ggggtaatcc aatactgatt ccctgcttgc
     1921 gcgggcaaag aatagcggtt atcggcatca aacaccaatt gaatccgaca ctctcctgag
     1981 caagaaacag gctcatctgc atacatcacc aattgtgctt ggtcaccttg cggcaagaac
     2041 aacaccaatt tcatttcttt aaacacactc gattcagaca agcgcttttt ggtggtaagc
     2101 atgtattctg ccccgtgtgc ttcgtcatgg gtcacattga tttgtgattg atgccaatcg
     2161 tttacccgtg cttgcacatc agcaccgtca ttaccgccgt cagtcatgcg ttcgcgctgt
     2221 ttggtttgct cttgcgattg tcgtatttca gcttctacac gtttttcgtt ggcatctttg
     2281 cgcaagtaaa acattcccac catacccaag accaaaataa tgacgatcac cagtacagga
     2341 atccaaaaag gtgtgtctga tttcaaagcc gagcgtggtc tcgcccgtga tgccgcattg
     2401 atgcccattt tcttgtcata gactttgcgc atttttgggt ctatcaagta attgcgcgct
     2461 tggctgagga ttttttcatc cacatctccc cgttgtcggg ccaaacgtat ggctttttca
     2521 atgtccatgg aagtggcatt ggtgggtatc cccaaggctt cgtaaaaatc aatcatgttt
     2581 tacctgacgg tttctgtgtg ttgatgtggt atgtgtcttg aaccaagctc aaagccttgt
     2641 gattgcttca agcctggcaa acgcaaaaga gcgtgaatgg gcaaatggct attgcctggt
     2701 tcatgtactc atctaggcat ccaagattta ggcagtttaa caaaaaagcg gcctgtaaag
     2761 atttacaggc cgcttttgcg agaataattt aagctgcgtg gtaaggtttg gacagtttat
     2821 gtaccgcgtc tatcaatact tttgcatgtt caggaggggt gtgttggttg atgccatgcc
     2881 ccaaattgaa cacatgacca ttgccatggc caaactcggc caaaatgcgc gccacttctt
     2941 gttctatgga ttcaggcgtg ccaaacaagg ccatggggtc gaaattgcct tgtaaggtca
     3001 ctttgtcgcc cacgcgccca cgcgctgcac ccaagtctac cgtccaatcc aatcccaaag
     3061 catccacgcc aatgtccgcc atcgcttcca accacaaacc accgcctttg gtgaaagcaa
     3121 tcacaggcac ttggcggcct tcggcctcac gggttaagcc tgccacaatt ttttgcatgt
     3181 aacgcaatga gaattcacgg taagccacat ggctgagcgc cccgccccaa gtatcaaaaa
     3241 tttgcactgc ttgcgcgcct gcggcgattt gcgcattcaa atacacaatc accgaatcgg
     3301 ccaacacatc caagattttg tgcaacaaat cgggacgctg gtacatcatg gttttgatga
     3361 ggcgaaactc tttgctgccg ccgccttcta tcatgtaaca cgccaaggta aacgggctgc
     3421 ccgaaaaacc aatcaaaggc acgcggccat tcaaagcctt gcggatgctg gaaacggcat
     3481 cggtgacata gcgcaatttg tccatatcgg ccacttgcaa agccgccaca tcggcctcag
     3541 tgcgtatcgg gcgttcaaac ttcgggcctt cgccctcagc aaaatacaaa cccaagccca
     3601 tggcatcagg tatggtcaaa atgtctgaaa acaaaatcgc cgcatccaaa tcgaagcgtt
     3661 ccaatggctg tatcgtcact tcggtggcca attcggtgtt tttgcacaaa gacaaaaaat
     3721 cgcccgcttg ggcacgggtg gcgcggtatt caggcaggta gcgccctgct tgacgcatca
     3781 tccacactgg cgtgtagtcc acatcttgtt tcaataaagc acgtaaaaac acatcgtttt
     3841 gcaattcggt catgggaggg ccgtcactaa aatcaatcaa atacgctatt ataggccaag
     3901 atgaccgcaa ttaccctgag ttaacgcaag tgtaggcaaa tatggtataa atcattccga
     3961 ttgagtaagc agtctagttc atttcatcac caaaccctta aggagccagc atgtctgaat
     4021 ctgaacaaaa cccgcaaaac ccagtaccaa cagaagcccc agcagcggtt caagccactt
     4081 cagccccaag cagcctcagc aaaaaatccg ctggcggtac cgacttgcgc caatacacga
     4141 tgattattta catcttgtac atggcagcga ttttggtggg catcacctcc atcgtgggcg
     4201 tcatcatggc ttatgtgaaa cgcgctgatt tcgcaggcac agagtatgaa gaccacatca
     4261 cttatttgat acgcaccttc tggatcagct tgattggtta tttgataggt gcggtgttgt
     4321 ccattgttgg catcggcatc atcatcatca tcgccgtggg catttggtac atctaccgtt
     4381 ctatcgcggg cttcatcaaa ttcaacgaca acaaacccat caatgccatg ggttggatgt
     4441 aagctgaatt gagcatctga acacaacaaa gcggcctgta aacatttaca ggccgctttt
     4501 tcatggttta aaacacccca aacctatgct attttggttt tacccatgcc aagtttttgc
     4561 atgccccaca tcacatagcc cgacaagctg taggccaaga agaacaagaa caacaccagc
     4621 gagggttccc agaacaacaa caagatggcc accacgatga tgagcatggt gaaaaatggc
     4681 actttgcggc gcacacccaa ttctttaaaa c
//
LOCUS       DAOMVK010000138         4695 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_5165_length_4695_cov_3.506897, whole genome shotgun sequence.
ACCESSION   DAOMVK010000138 DAOMVK010000000
VERSION     DAOMVK010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5165_length_4695_cov_3.506897"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..2232)
                     /gene="pnp"
                     /locus_tag="PK856_05820"
     CDS             complement(115..2232)
                     /gene="pnp"
                     /locus_tag="PK856_05820"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRL01921.1"
                     /translation="MFNKHVKTFQYGNQTVSIETGEIARQATAAVKVSMGDTVVLVSV
                     VAAKKIKEGQDFFPLTVDYFERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRPL
                     FPEGFYNDIQIIAMVVSVDPEIDSDIPAMIGASAALVLGGVPFAGPIGAARVGYVNGD
                     YVLNPTKAQLTESQLDLVVAGTDKAVLMVESEAKELTEAVMLGAVVYGHEQMQTVITA
                     INELADEVNPAVWEWTAPVENAELKAKVKELAGGIVAEAFKIRSKQERTVKLGEAWTA
                     LEAALITEETSTLEVNEIRTLFKHLEGEVVRGQILDGQPRIDGRDTRTVRPIDIQTSV
                     LPRTHGSSLFTRGETQALATATLGTQRDEQIIDALSGEYTDRFIMHYNFPPYSTGEVG
                     RVGIPKRREIGHGRLAKRALIGVLPAQEDFNYTMRVVSEITESNGSSSMASVCGGCLA
                     LLSAGVPLKAHVAGIAMGLILEGSKFAVLTDILGDEDHLGDMDFKVAGTENGITALQM
                     DIKIQGITKEIMHIALEQAKEGRAHILGLMKEAVEGPQEMSTHAPRLYTLKINQDKIR
                     DVIGKGGETIRGIIKEFGTEINIDDDGTVVIAATTAEAGEAAKKRIEEITAEVEVGKT
                     YSGTVLKILDNNVGAIVSVMPGKDGLVHISQIAHERVKNVSDYLQVGQTVDVKALEVD
                     DRGRVRLSIKALLEKPAEPAAAE"
     gene            complement(2403..2672)
                     /gene="rpsO"
                     /locus_tag="PK856_05825"
     CDS             complement(2403..2672)
                     /gene="rpsO"
                     /locus_tag="PK856_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRL01922.1"
                     /translation="MALTVEQKAQIVKDFQRKENDTGSSEVQIALLTFRINDLTPHFK
                     ANLKDHHSRRGLLKMVSQRRRLLSYLRRTDAETYRSVITRLGLRK"
     gene            complement(2757..3671)
                     /gene="truB"
                     /locus_tag="PK856_05830"
     CDS             complement(2757..3671)
                     /gene="truB"
                     /locus_tag="PK856_05830"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRL01923.1"
                     /translation="MTTAKPPKRRINGVLLLDKPKGKSSNTALQISKRLFQAEKAGHT
                     GILDPLATGLLPVCFGEATKYAQYLLDSDKAYIADLQLGIETTTGDVEGEISSHSDAR
                     ITAEAFQSACVAYTRVYEQMPPMFSALKHNGKALYEYARQGINIERKRRTITIFSIEV
                     LAFDEAAQTAQIAVSCSKGTYIRTLAEDIAREAGTLAHLTDLRRTATAGFDISQSHDI
                     EAIEGLELDGRDALLLPCEVLVTHLPAITLEDAEIVRLRYGQQVQTAVNYGIMSALRV
                     YDVNQTFVGLVEALPEMRLKALRLMSTN"
     gene            4089..4430
                     /locus_tag="PK856_05835"
     CDS             4089..4430
                     /locus_tag="PK856_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01924.1"
                     /translation="MQQYQNTFWLLQGYKGNENSVAYIQTAVAEEPNLLHEDPSFAVK
                     EGEQVLVLAKERQIPSFIGCALSAVLWRKLSWDEKVFMWVYEHRWAIEVGIVLLMVVT
                     VKRWRERRMRA"
     gene            complement(4504..>4695)
                     /locus_tag="PK856_05840"
     CDS             complement(4504..>4695)
                     /locus_tag="PK856_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01925.1"
                     /translation="YHRLQRESHIDKPYFTQEAQNMFILLPTWQWQASDVSQAIHTYW
                     AMHVEGFPLAHQGNASKLA"
BASE COUNT         1139 a         1073 c         1191 g         1292 t
ORIGIN      
        1 gaaaggtgtt ttggtatttg gagtctagca aagaatggtg tgttcagtat gaaattgcca
       61 tgaaaaaacc gcctgaaaac tcaggcggtt gcattttttg accttacagg ccgcttattc
      121 tgctgctgca ggctctgctg gtttttccaa caaggctttg atagacaagc gcacacgacc
      181 gcggtcgtct acttccaagg ctttcacgtc tacggtttga cccacttgca agtagtcaga
      241 cacgtttttc acgcgttcgt gagcgatttg gctgatgtgt accaagccgt ctttgccagg
      301 catcacagac acgatggcgc caacgttgtt gtccaagatt ttcaagacag tgccagagta
      361 ggttttgccc acttcaactt ctgcggtgat ttcttcgatg cgttttttcg cggcttcgcc
      421 tgcttcagca gtggtcgctg caatcaccac ggtgccatcg tcatcaatgt tgatttcagt
      481 accgaattct ttgatgatgc cgcggatggt ttcaccacct ttgccgatta catcgcggat
      541 tttgtcttgg ttgattttca aggtgtacaa acgcggtgcg tgagtagaca tttcttgagg
      601 accttcaacc gcttctttca tcaaacccaa gatgtgtgca cggccttctt tggcttgctc
      661 caaagcgatg tgcatgattt ctttggtgat gccttggatt ttgatgtcca tttgcaaagc
      721 agtgatgccg ttttcggtac ccgctacttt aaagtccatg tcgcccaagt ggtcttcgtc
      781 gcccaagatg tcggtcaaga ctgcaaattt agagccttct aaaatcaagc ccatggcaat
      841 accagccacg tgtgctttca aaggcacgcc agctgacaac aaggccaagc aaccgccaca
      901 cacagaagcc atagaagaag agccgttaga ctcggtgatc tcagacacca cacgcatggt
      961 gtagttaaaa tcttcttgtg ctggcaacac gccaatcaac gcacgtttgg ccaagcggcc
     1021 atgaccgatt tcgcggcgtt taggaatgcc cacgcggccc acttcacctg tagagtatgg
     1081 tgggaagttg taatgcatga tgaagcggtc ggtgtattcg ccgctcaaag cgtcaatgat
     1141 ttgctcatcg cgttgcgtgc ccaaagtggc tgtggccaag gcttgggttt caccgcgggt
     1201 aaacaaagaa gaaccgtggg tgcgtggcaa cacgctggtt tggatgtcga tagggcgtac
     1261 ggtgcgggta tcgcgaccgt caatgcgtgg ttgaccatcc aagatttgac cgcgaaccac
     1321 ttcgccttcc aagtgtttga acaaggtgcg aatttcgttc acttccaaag tgctggtttc
     1381 ttcggtaatc aaagccgctt caagtgctgt ccaagcttcg cccaatttca ccgtgcgttc
     1441 ttgttttgag cggattttga aggcttcagc cacgatgccg ccagccaatt ctttgacttt
     1501 ggctttcaat tcagcgtttt caactggcgc agtccattcc caaaccgcag gattcacttc
     1561 gtcagccaat tcgttgatgg cagtaatcac ggtttgcatt tgctcgtgac cataaaccac
     1621 cgcacccagc atcaccgctt cagtcaattc tttggcttca gattccacca tcaatacggc
     1681 tttgtcagta cctgctacca ccaagtccaa ttggctttcg gtcaattggg ctttggtagg
     1741 gttcaaaaca taatcgccat tcacatagcc cacgcgtgct gcaccgatag ggcctgcaaa
     1801 aggcacgccg cccaatacca aggcagcaga agcaccgatc atcgcaggga tgtcagaatc
     1861 gatttctggg tctacagaca caaccatggc aatgatttga atatcgttgt agaagccctc
     1921 tgggaataaa ggacgaatcg gacggtcgat caaacggctg gtcaaaattt ctttttcaga
     1981 ctgtttgcct tcgcgtttga agaagccacc agggatttta ccggccgcat acgtgcgctc
     2041 gaaataatcc acggtcaatg ggaagaagtc ttggccttct ttgatttttt tggctgctac
     2101 cacagacacc aataccacgg tgtcacccat ggatacttta acggctgctg tggcttggcg
     2161 agcgatttcg cctgtttcga tgcttacggt ttggttgccg tattggaatg ttttaacgtg
     2221 tttgttaaac atgaatatcc tttgaaaagt gtcggatacc gacctgaatc actaatgatg
     2281 cacacaaagc ataaaccaat gtgcatggtt agttattcag aatcgcatcc tcattaaata
     2341 aaaaaccgtt ctagatagaa caaagtcgca gcgtctgctg cgactttaag gtaaaaccta
     2401 acttatttac gcaagcccaa acgggtaatc acgctgcggt aagtttccgc atcagtacgg
     2461 cgcaagtagc ttaacaagcg acgacgttgg ctcaccattt tcaacaaacc acgacggctg
     2521 tggtggtctt tcaagttggc tttgaagtga ggcgtcaagt cattgatacg gaaagtcaac
     2581 aaagcaattt gtacttcgct tgaaccagta tcgttttctt tgcgttggaa atctttaacg
     2641 atttgcgctt tttgttctac ggttaatgcc atttgtaatg ctccaaatat aaaaatgggt
     2701 tttcaaaccc gacaagacaa cagagccgaa accctcgctg tctccaaact acgtgtttaa
     2761 ttcgtgctca tcaagcgcaa ggcttttaaa cgcatctcag gcaaagcttc caccaagcct
     2821 acgaaggtct gattcacatc ataaacccgt aaggccgaca ttattccata attcaccgcg
     2881 gtttgcacct gttgtccgta tcttaaacgc acaatttctg cgtcttccag tgtaatggct
     2941 ggtaaatgtg tcaccaaaac ctcacaaggc aggagcaaag catcgcggcc atccagttcc
     3001 aaaccctcga tggcctcaat gtcatggctt tggctaatgt caaaacctgc ggttgcggtg
     3061 cggcgcaaat cggttaagtg ggcgagtgtg cctgcttcac gggcaatgtc ttcggccaaa
     3121 gtacggatgt aagtgccttt ggagcaagaa acagcgattt gggcggtttg cgcggcttca
     3181 tcgaaagcca atacttctat gctgaagatg gtgatggtgc ggcgtttgcg ctcaatgttg
     3241 atgccttgtc tggcgtattc gtacagtgct ttgccattgt gtttcaaagc agaaaacatc
     3301 ggtggcattt gctcatacac gcgggtatag gccacacaag cggactgaaa cgcttcggca
     3361 gtgatgcgcg catcgctgtg agaggagatc tcaccctcca catcgccagt ggtggtttca
     3421 atgcccaatt gcaaatcggc aatataagct ttgtcggagt cgagcaggta ttgtgcgtat
     3481 ttggtggctt caccaaaaca tacgggcaat aagcctgttg ccaaagggtc gagtatgcca
     3541 gtgtggccgg ctttctcggc ttggaacaga cgtttgctga tttgtaaggc ggtattgctg
     3601 gatttgcctt tgggtttgtc caaaagcaac acaccgttga tgcggcgttt gggtggtttt
     3661 gctgtggtca tgcaggtttg gttacactgt tttacgtggg cgagagtgta gcatgatttg
     3721 tgttagttgc cgttgttgcc tgtccttaat tggcataagc tgcgtgtttc atgactaagt
     3781 caggttttga tttgttagcg ttcatgaatg gattggtaat tggattgtgt gacgcagtaa
     3841 atgctacgct gataaggcga attgtcaggg ttttgatgcc acctgttttg ttcaaggtaa
     3901 aatggtttac aggccgcttt aattggaggt gagctgggta tggtactttt aggaagatgc
     3961 aatatgatga agaaacagtg caaggcttgg ctattgggag cgggtgtgct gttgtctgtg
     4021 ttaagccagt atgcgtaagc cacagtctgt accaacacac cttctctcaa cacacagacc
     4081 aaaacaatgt gcagcagtat caaaacacgt tttggctctt acaaggctat aaaggcaatg
     4141 aaaacagcgt ggcttacata caaaccgctg tggcagaaga gccaaacttg ctgcacgaag
     4201 acccgagttt tgcggtgaaa gagggtgaac aggttttggt tttggcaaaa gaaaggcaaa
     4261 taccaagttt cataggatgc gccctctctg cggttttgtg gcgaaagttg tcatgggatg
     4321 aaaaagtgtt tatgtgggtg tatgagcatc gctgggctat tgaggttgga atcgtgttgc
     4381 tgatggtggt tacagtaaaa agatggcgcg agcggcgtat gcgcgcttga tgattgcctt
     4441 agcttggcat tgaactggaa catttaactg gtttaatgat ttataggccg cttgtgtgga
     4501 tacctaggcc aatttagaag cattgccttg gtgggctaag ggaaagcctt ccacatgcat
     4561 ggcccaataa gtatggatgg cttgggaaac gtcactggcc tgccattgcc aagtgggcag
     4621 cagaataaac atattttgtg cttcttgggt gaaatacggc ttatcaatat gggattcgcg
     4681 ctgtaagcgg tgata
//
LOCUS       DAOMVK010000139         4694 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_5167_length_4694_cov_3.511748, whole genome shotgun sequence.
ACCESSION   DAOMVK010000139 DAOMVK010000000
VERSION     DAOMVK010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5167_length_4694_cov_3.511748"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1680
                     /locus_tag="PK856_05845"
     CDS             <1..1680
                     /locus_tag="PK856_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated helicase Cas3"
                     /protein_id="HRL01926.1"
                     /translation="LVCTIDHMMAATETVRGGKYILPCLRLLSSDLVIDEVDDFNPQD
                     LVAIGRLVHLAGMLGRKVMISSATIPPALAEGFFNVYQQGWHLHCQFKQLTPKVVGMW
                     VDEFNSHISETVPEQEQTLPVYREAHNQFVQARLKQLQQALVKRKALVVRCEQVFDTD
                     SDEGKRQQYFELVRAQIEQLHLHHHHVDAKTGKKVSFGVVRVANINPCVLLSRYLINA
                     DWSESFAPKVMAYHSRQILLLRQQQEAYLDAVLNRKRPVNEGVLQNPAIRAHLDDTDA
                     EHVVFVLVATPVEEVGRDHDFDWAVIEPSSLRSIIQLAGRVLRHRQREATTPNIALMQ
                     YNLRALMQGKNSSKAAFCRPGFEPDGELRLLSHDVCDILNTEQLAHGIDAQARIQAAE
                     SLQPRQKLADLEHATMQKSLADLGQKGPMALPSWSRDVWFLTGLPQRFNRFRESSANI
                     QLFATVQNGRMVWQERDGYGEYVNRNQTYTVAYETFSDSQKQRMWLWRDYAQALEDRL
                     TAFETDIEKALLRQAHRYGEIMLPDFDKNKELMYSDQFGLSTAMEMEGEVE"
     gene            1677..2921
                     /gene="csy1"
                     /locus_tag="PK856_05850"
     CDS             1677..2921
                     /gene="csy1"
                     /locus_tag="PK856_05850"
                     /inference="COORDINATES: protein motif:HMM:NF021151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated protein Csy1"
                     /protein_id="HRL01927.1"
                     /translation="MTQAVMEYLAERRKNWLDKRLSGGMDEAEALALETEANEKFSLA
                     QWLPDAARRAGHLSMVSHPSKFSHPSAKTSAVIAQASLQADGYVRTGNVHYPLDVFGS
                     AASIDVYKFLALPLSDGKTVLEHLEQETALAQQLLSQAPSVPVSELTSQFLAIKASET
                     QVKTDGLVKQVYFPVDGAYHLLSILTPSGMLTEFKERIDAMRFSDASKEAREARKHQL
                     YHAQGFNDILDLTVTVYGGSKPQNISVLNNQNSGRSYLLPCKPPKLDHRATRLPSRDF
                     FKQTLFVRDFDVYFKSLFELMALDINNMHIRAGIRKVLHKIVDEVMYRAFCVREFAGG
                     WSQTEHYQALPKAQQLWLDAALLEKRLEDDEWQEAIAKALRQWLAGEIERRQNHIKLG
                     DAEQLYLQATIVEVLAADKEYF"
     gene            2921..3844
                     /gene="csy2"
                     /locus_tag="PK856_05855"
     CDS             2921..3844
                     /gene="csy2"
                     /locus_tag="PK856_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated protein Csy2"
                     /protein_id="HRL01928.1"
                     /translation="MRPFLLIPHIRIHNANAMSSPYSIGFPAMTAWLGAVHALQRKLQ
                     QLPDFENLCLSKVGISCHQLEVQTHRSKGDFVSSIIGTGNPLDKDGSRPSFIEEARCH
                     VTVSLLIEMSESDPEWEESLPIAIAPMLRQMKMAAGDVLSFREPQVLYLEEGNASKLK
                     ILMQRLMLGHVLVERRQLLQDYVNQNPEQDVLHALLAHLHIRHRVAVDANQEAIAIGN
                     NQYLWEASRLQAGWLVPIAVGFQGISPLGLAQNQRDANTPHRFAESVLTLGEFVMPYR
                     IERIDDMLWHYHVDAEQDLYVCQTSVASLEN"
     gene            3860..>4694
                     /gene="csy3"
                     /locus_tag="PK856_05860"
     CDS             3860..>4694
                     /gene="csy3"
                     /locus_tag="PK856_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated protein Csy3"
                     /protein_id="HRL01929.1"
                     /translation="MAKEKNVAVASVLSFEKKLVPSDAYLYGTTWDERADKATALPLV
                     EKSVRGTISNRLKDAIKKDPLKLNAEVEKPNLQRVDACSLSEDQDTLKLHFTLKVLGD
                     IAKPSACNNEEFKNSYAEAVTQYQTERGFGELARRYAINIANGRYLWRNRLGAEKVEV
                     VVKAGEQTWTFDGLAYALNRFDASDAQLDALTEVIRQALSGELTYLLLDISAYALVGA
                     AQEVYPSEELVLDKGNDKNKKSKILYAKNGIAAMHSQKVGNALRSIDTWYPEFDTAQM
                     AI"
BASE COUNT         1219 a         1009 c         1311 g         1155 t
ORIGIN      
        1 ttggtttgca ccatagacca catgatggcg gccacagaaa ccgtgcgcgg tggcaaatac
       61 atcttgccgt gtttgcgctt attgtcttcg gatttggtga ttgatgaagt cgatgatttc
      121 aatccacaag acttggttgc gattggccgc ttggtgcatt tggcgggcat gttgggccgc
      181 aaggtgatga tttcttcggc aacgataccg cccgctttgg ccgagggctt tttcaatgtg
      241 taccaacagg gttggcattt gcattgccaa ttcaaacagc tcacacccaa agtggtgggt
      301 atgtgggtgg acgaatttaa cagtcacatc agcgaaacgg tgccagaaca agagcagact
      361 ttgcctgtgt accgcgaagc gcacaaccag tttgtgcaag cacggctcaa acaattgcaa
      421 caagcattgg tgaagcgcaa agcgctggtg gtgaggtgtg agcaggtgtt tgataccgac
      481 agcgatgagg gcaaacgcca acagtatttt gagttggtgc gcgcgcaaat cgaacaattg
      541 catttgcacc accaccatgt cgatgccaaa acgggcaaga aggtgtcgtt tggcgtggtg
      601 cgggtggcga acatcaatcc gtgtgtactg ctcagccgtt atttaatcaa tgccgattgg
      661 agcgaaagtt ttgctcccaa ggtgatggcg tatcacagtc gtcaaatttt gttgttgcgg
      721 cagcagcaag aggcttattt ggatgcggta ttgaatcgca agcggcctgt aaacgagggt
      781 gtgttacaaa atcctgccat acgcgcacat ttggatgata ccgatgccga gcatgtggtg
      841 tttgtgttgg tggcgacgcc tgtagaagaa gtggggcgcg accacgattt tgattgggcc
      901 gtgatagagc cttcatcgct gcgctccatc atccaattgg cagggcgcgt gttgcgccat
      961 cgccaacgcg aggccaccac gccgaatatt gcgctgatgc aatacaattt gcgggcgctc
     1021 atgcaaggga aaaactcaag caaagccgca ttttgtcgcc ctggctttga gccagatggt
     1081 gagctgcgtt tgctcagtca cgatgtgtgc gacattttga ataccgagca attggcgcac
     1141 ggtatcgatg cacaggccag aatacaggcc gctgaatcct tgcagcctag gcaaaaattg
     1201 gccgatttgg aacacgccac catgcaaaaa agcttggctg atttgggcca aaaaggcccg
     1261 atggctttgc caagttggag ccgtgatgtg tggtttttaa ccggtttgcc gcagcggttt
     1321 aaccgctttc gcgaatcaag tgccaacata caattgtttg ccaccgtgca aaatgggcgc
     1381 atggtgtggc aagagcgtga tggctatggt gagtatgtca atcgcaatca aacctacacc
     1441 gtggcctatg aaacattcag cgacagccaa aaacagcgca tgtggctgtg gcgtgattat
     1501 gcccaagctt tggaagatag gctgacggca tttgaaacag acattgaaaa agcgctgctg
     1561 aggcaggcac accgctatgg tgaaatcatg ttgcccgatt ttgataaaaa caaagagttg
     1621 atgtattcag accaatttgg cttaagcacg gccatggaga tggagggaga ggtggaatga
     1681 cgcaagcggt gatggagtat ttggccgaaa gacgtaaaaa ttggctggac aagcgcttaa
     1741 gtgggggcat ggatgaggcc gaagcattgg cattggaaac agaggcgaat gagaagtttt
     1801 cattggcaca gtggttgcct gatgcggcaa ggcgggcagg tcatctgtct atggtcagcc
     1861 atcccagtaa attcagccat cccagtgcca aaacatcagc agtcattgct caagccagtt
     1921 tacaggccga tggttatgtg agaacaggca atgtgcatta tccattagat gtgtttggca
     1981 gcgctgcttc gattgatgtg tacaaatttt tggcgttgcc tttatcagat ggcaaaaccg
     2041 tgttggagca tttggagcaa gaaacggcat tggcacaaca gctgttgtcg caagcgcctt
     2101 ccgtgcctgt gtcagaattg acctcacagt ttttggccat caaggccagt gaaactcagg
     2161 tgaaaaccga tggtttggtg aagcaagtgt atttccctgt ggatggtgcg tatcacttgc
     2221 tgtcgatact gacaccctcg ggcatgctca ctgaatttaa agagcgaatt gacgcgatgc
     2281 gtttttctga cgcgagtaaa gaagcgcggg aagcgcgtaa acatcagctc taccatgccc
     2341 aaggtttcaa tgatattttg gatttaaccg ttacggtgta tgggggcagt aagccacaaa
     2401 acatcagtgt attgaacaat caaaacagtg gtcgcagtta tctgttacct tgcaaaccac
     2461 ccaaactaga ccaccgcgcc acccgtttgc ccagccgtga tttctttaag caaactttgt
     2521 ttgtgcgaga ctttgatgtg tatttcaaat cgctgtttga attgatggct ttggacatta
     2581 ataacatgca cattcgcgca ggcatacgca aagtgttgca caaaattgtg gacgaagtga
     2641 tgtaccgtgc gttttgtgtg cgcgaatttg caggtggatg gagccaaact gagcattatc
     2701 aggctttgcc taaggcacaa cagctgtggt tggatgcggc attgcttgaa aagcggttgg
     2761 aagacgatga gtggcaagag gccattgcta aagccttgcg gcagtggttg gcaggtgaaa
     2821 ttgaacgcag acaaaaccac atcaaattgg gcgatgcgga acagttgtat ttacaggcca
     2881 ctattgttga ggtgttggct gcggataagg agtattttta atgcgcccat ttttattgat
     2941 accacacatc cgcattcaca atgccaacgc gatgagcagt ccttacagca ttggttttcc
     3001 tgcgatgacg gcatggttgg gtgcggtgca tgccttgcag cgcaaattgc aacagctgcc
     3061 agattttgaa aacctgtgtt tgagcaaagt ggggatttct tgtcaccagt tggaggtgca
     3121 aacccaccgc agcaaaggcg acttcgtgtc gtcgattata ggtacgggca atcctttgga
     3181 caaagatggc agccgacctt cgtttattga agaggcacgt tgtcatgtca ccgtgtcctt
     3241 gttgattgaa atgtctgaaa gcgaccctga atgggaagaa agcttaccca tcgccattgc
     3301 gcccatgctg cgccaaatga aaatggccgc aggcgatgtg ttgtcgtttc gagagccgca
     3361 agtcttgtat ttggaagaag gcaatgccag caaattgaaa atactgatgc agcgcctgat
     3421 gctcggccat gtgttggtag agcggcgtca attgttgcag gattatgtga atcaaaatcc
     3481 tgagcaagat gtgttgcatg ccttgctggc gcatttgcac atccgccacc gtgtggctgt
     3541 ggatgcgaat caagaagcga ttgcgatagg caacaaccaa tacctgtggg aggcgagtcg
     3601 tttacaggcc ggttggttgg tgccgattgc ggtaggtttt caaggcattt cacctttggg
     3661 tctggctcaa aatcagcgcg atgcgaacac gccgcaccga tttgccgaaa gtgtgttgac
     3721 cttaggcgaa tttgtgatgc cttaccgtat cgagcgcata gacgacatgc tgtggcatta
     3781 ccatgtggat gcagaacaag atttatacgt ttgtcaaacc agtgttgcaa gtttagaaaa
     3841 ttaattcagg gagaaaatca tggcaaaaga aaaaaatgtg gcagtggcca gtgtgttgtc
     3901 gtttgaaaag aaattggtgc cttctgatgc gtatttatat ggcacgactt gggatgaacg
     3961 tgctgacaaa gccaccgctt tgcccttggt agaaaaatcg gtgcgcggca cgatttccaa
     4021 tcgcttgaaa gacgcgatta aaaaagaccc attgaaactg aatgctgaag tggaaaaacc
     4081 caatttgcag cgcgtggatg cttgctcatt gagcgaagac caagatacct taaaactgca
     4141 ttttaccttg aaagtattgg gtgatatcgc caaaccatcg gcctgtaaca atgaagaatt
     4201 taaaaacagc tatgccgaag cggtgacgca ataccaaaca gagcgcggct ttggtgaatt
     4261 ggcgcgccgt tacgccatca atattgccaa tggccgctat ttgtggcgca accgtttggg
     4321 tgcggaaaaa gtggaagtgg tggtgaaagc tggtgagcaa acgtggacgt ttgatggttt
     4381 ggcctatgct ttaaaccgct ttgatgccag cgatgcgcaa ttggatgcct tgaccgaagt
     4441 gatacgccaa gccttgagcg gggagctgac ttatttgctg ctcgacatca gtgcttacgc
     4501 tttggtgggg gcagcacaag aggtttaccc aagcgaagag ttggtgttgg acaaaggcaa
     4561 tgacaagaat aaaaagagca aaattcttta cgccaaaaat ggcatcgccg ccatgcacag
     4621 ccaaaaagtg ggcaatgccc tgcgcagcat agacacttgg tatcctgaat ttgacacggc
     4681 acaaatggcg attg
//
LOCUS       DAOMVK010000140         4647 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_5247_length_4647_cov_3.180967, whole genome shotgun sequence.
ACCESSION   DAOMVK010000140 DAOMVK010000000
VERSION     DAOMVK010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5247_length_4647_cov_3.180967"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..1303)
                     /gene="ccsB"
                     /locus_tag="PK856_05865"
     CDS             complement(116..1303)
                     /gene="ccsB"
                     /locus_tag="PK856_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003759502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HRL01930.1"
                     /translation="MKTDNISLPKHDYLTETRLLHRLAWRDWVFAVVIFAAALYAQLF
                     IAEHHMDVYEMAILWGSALGVVALGWFFKPMQWFVPVSAALAWLSMQVYAGDMARAEQ
                     AFLLKYFLASQSAIMWLCFFTVMAFLCFSAGLLQAKMRPVSSHDTLFSIATGFAWTAS
                     VFGFVGLLVRWHESYLLRPDAGHIPVSNLYEVFILFMVITALMYLYYAARYQAAKLGA
                     FVYGFMCLLVGFVLWYSFDRGAHQIQPLIPALQSWWMKIHVPANFIGYGAFCMAAALG
                     IAELLALRSKGNSVLPSAEVIDEIMYKAIAVGFLFFTIATVLGALWAADAWGRYWSWD
                     PKETWALIVWLNYAIWLHLRLVAGWRGKVLAWWAIIGFFITAFAFLGVNMFLSGLHSY
                     GTL"
     gene            complement(1300..3300)
                     /locus_tag="PK856_05870"
     CDS             complement(1300..3300)
                     /locus_tag="PK856_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HRL01931.1"
                     /translation="MKKAVPLIRRPWFAFLSSMRFAVALLTLLAIASVVGTVLQQNQP
                     VADYVIKFGPFWFEIFKFLGLFDVYASGWFVLIMLFLVVSTSLCLWRNIPPFWREMRS
                     YRLKATAQSLGAMKHSSLLTGRLSPEVLTRYLNVQGFQVKQQARDDGSVILAAKKGSA
                     SKLGYIFAHVAIIVICLGGLIDSNMGLNLGVLSGRIVPDKDTLLAKDFAPNARLDSGS
                     MSFRGNVTIAEGKGADVVFLNAGDGLVVQELPFSVELNKFHVEYYDTGMPKNFASDLT
                     IIDHKTGEKKQATIKVNHPLIVDGVAIYQASFGDGGSALSFNAWDTRNALLNSAPIQA
                     TSVNTFPLNMNGQEYRLEFGEFRMLNVEDQSLPEAEKSGFTQKLHDVRDVNKANELKN
                     VGPTITYKVRDKAGQAVEYMAYMLPLSREEQQFYALGERVSPADPYKWVMMPVDRNGE
                     LKTFMLLRQGFADAKLRERAVSKAMNSVSEQIRPQFAVAVDNVLRLFSEGGYVALDAF
                     VAQNVPETEQERMRQFFLEVLQASSGYVLDEVLLQAGEPAWADSPERSRFIASASVGM
                     TGMAQVKAPLYLQLTEFKHVQASGLQMAKSPGRYLVYLGSLLLILGTLCMFYVREKRL
                     WVLVNQDNMRVAMSATRHARDLESEFPQHVARINQLVKDLQQ"
     gene            complement(3402..4013)
                     /locus_tag="PK856_05875"
     CDS             complement(3402..4013)
                     /locus_tag="PK856_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRL01932.1"
                     /translation="MKRLSWVAVTLLFSAWAGAAGPAANLERGKQVATNVCAACHAVD
                     GNSGIATYPRLAGQNAFYIEHNAKLIRDGKRTTGNAKEMRDQMGAVSDQDLKDAALYF
                     SKQYPKAGEVNPKNNPELGAKIFRGGIAERKVPACMSCHGPNGAGMPNEYPRISGQHA
                     GYVETELKAYRDGVRKNAKMEKVAKMLTAEEMKAVANFIQGTY"
     gene            4145..>4647
                     /gene="yihA"
                     /locus_tag="PK856_05880"
     CDS             4145..>4647
                     /gene="yihA"
                     /locus_tag="PK856_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003786053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HRL01933.1"
                     /translation="MPLFQNAQFFTTVNHLKDLPPTQAEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELRNGHYLADLPGYGYAQVPEDVRAHWVRLLGQYLQQ
                     RQSLIGLILIMDCRHPLKELDKQMLDFFAVTGHPVHILLSKADKLSKNDQIKTLSSVK
                     KALKPYM"
BASE COUNT         1305 a         1309 c          975 g         1058 t
ORIGIN      
        1 aatgactcga accatcttca ttcatactgt tgccgcctcc actaaaatat tgatgaagct
       61 cagtttaaca gttcgcaagc tcggtgcgca agttttcaca aaccgctcta ttcacttaaa
      121 gcgtgccata ggaatgcaaa ccgcttaaga acatgttcac acccaaaaaa gcaaacgcag
      181 taatgaaaaa gccgataatg gcccaccaag ccaacacctt accgcgccaa cctgccacca
      241 aacgcaggtg caaccaaatg gcgtaattga gccacacaat caaagcccaa gtttctttcg
      301 ggtcccaaga ccaataacgt ccccatgcat cggccgccca caaagcaccc aacaccgtgg
      361 caatggtaaa gaacaaaaag cccacggcta tggctttgta catgatttca tctatcactt
      421 cagccgatgg caacactgaa ttgcctttgc tgcgcaaggc caacaattcg gctatgccca
      481 aggccgctgc catacagaac gcaccgtaac caatgaaatt ggcaggcaca tggattttca
      541 tccaccatga ttgcaaggcc ggaatcaaag gctgaatttg gtgtgcaccg cgatcgaagc
      601 tgtaccacaa cacgaaaccc accaacaaac acatgaagcc atacacaaat gcacccagct
      661 tggcggcttg gtaacgggcg gcgtaataca gatacatcaa ggccgtaatc accatgaaca
      721 agatgaacac ttcatacaaa ttcgacacag gaatgtggcc cgcatcaggg cggagcaaat
      781 agctctcatg ccaacgcacc aacaagccga caaaaccaaa cacactggcc gtccaagcaa
      841 agcctgtagc gatgctgaac aaggtgtcat gtgagcttac aggccgcatc ttcgcttgca
      901 acaagcctgc gctgaaacac agaaacgcca tcaccgtgaa aaaacacaac cacatgatgg
      961 ccgattggct ggccaaaaaa tacttcagca aaaacgcctg ttcggcgcgg gccatgtctc
     1021 ccgcatacac ttgcatcgat aaccaagcca aggccgcgct gacgggcaca aaccattgca
     1081 tgggtttgaa aaaccaaccc aaagccacca cgcccaaggc cgagccccac aaaatggcca
     1141 tttcatacac atccatgtgg tgctctgcaa tgaacaattg tgcatacaat gcagcggcaa
     1201 aaatcaccac ggcaaatacc caatcgcgcc aagccaggcg gtgcaacaaa cgggtttcgg
     1261 ttaaataatc gtgtttgggc aggctgatgt tgtctgtttt cattgctgca aatccttcac
     1321 caactgattg atgcgcgcca catgttgtgg aaattcactt tctaaatcgc gggcatgtcg
     1381 ggtagcgctc atggccacac gcatattgtc ttgattcacc aatacccaca agcgcttttc
     1441 acgcacataa aacatgcaca aggttcccaa aatcaacagc aaagaaccca aatacaccaa
     1501 atagcggcct ggagatttag ccatttgcaa acccgaagcc tgcacatgtt tgaactctgt
     1561 gagttgtaaa tacaatggtg ctttcacctg tgccatacct gtcatgccca cactggcact
     1621 ggcaataaag cgagagcgct caggtgaatc ggcccaagcg ggctcacccg cttgcagcaa
     1681 cacctcatcc aacacatagc cgctgctcgc ttgcaacact tccaagaaaa actggcgcat
     1741 gcgctcttgc tcggtttctg gcacattttg cgccacaaac gcatccaagg ccacatagcc
     1801 gccttcggaa aacaatcgca acacattgtc taccgccaca gcaaattgcg gtcgaatttg
     1861 ttcgctcacg ctattcatgg ccttactcac cgcccgctca cgcaatttgg catcggcaaa
     1921 accttgacgc aataacatga aagttttcaa ttcaccattg cggtcaactg gcatcatcac
     1981 ccacttgtaa gggtcggcag gcgatacacg ctcacccaag gcataaaact gttgttcttc
     2041 ccggctcagg ggcaacatat acgccatgta ttccactgcc tgccctgctt tgtcacgcac
     2101 tttgtaagtg atggtagggc ccacattttt cagctcattg gccttattca catcgcggac
     2161 atcgtgtagt ttttgagtaa accctgattt ttcagcttca ggcaaagact ggtcttccac
     2221 attcaacatg cgaaattcgc caaactccaa acggtattct tggccattca tgttcagtgg
     2281 gaaggtgttc actgaagtgg cttgaatggg cgctgaattg agcaaggcat tgcgcgtgtc
     2341 ccaagcattg aaactcaagg cagagccacc atcaccaaaa ctggcttggt aaatcgccac
     2401 cccatcgaca atcaatgggt ggtttacctt gatggttgcc tgtttttttt cacctgtttt
     2461 gtggtcaata atggttaagt cactggcaaa gtttttaggc atgcctgtgt cgtaatactc
     2521 aacatgaaac ttgttcagct ctaccgaaaa tggcagctct tgcaccacca aaccatcgcc
     2581 tgcattcaaa aacaccacat cagcgccctt gccttcggcg atggtgacat tgccacgaaa
     2641 cgacatgctg cctgaatcta aacgggcatt gggggcaaag tctttggcca acagagtgtc
     2701 tttatcgggc acgatgcggc ctgacaacac acccaagttc aagcccatat tgctgtctat
     2761 caagccaccc aagcaaatca cgatgatggc cacatgggca aaaatataac cgagcttgct
     2821 ggcgctgcct tttttcgccg ccaatatcac cgaaccgtcg tcacgggcct gctgtttaac
     2881 ttgaaagcct tgcacattca gataacgtgt gagcacttct gggcttaagc ggcctgtaag
     2941 caagctgctg tgcttcatcg cacccagtga ttgggctgtg gctttcaaac ggtatgagcg
     3001 catttcacgc caaaatggtg gaatattacg ccacaaacac aagctggtcg aaaccaccaa
     3061 aaacaacatg atgagcacaa accaaccaga tgcatacaca tcaaacaaac ccaaaaattt
     3121 gaaaatctca aaccaaaatg gcccaaactt aatcacataa tccgcaacgg gttgattctg
     3181 ttgcaataca gtgcccacca ccgaagcaat ggccaataag gtcaacaagg ccacggcaaa
     3241 gcgcatcgag cttaaaaacg caaaccacgg acgacggatg aggggaaccg cttttttcat
     3301 aaactccaac ctgtggcaaa ctgcctcggt taaaaaataa aacaagaccg ccacatcatg
     3361 atatgcgcgg tctgtaggca tgcattaact gccagcagca atcaatacgt gccttgtatg
     3421 aagttggcta ccgctttcat ttcttctgca gtcagcattt tagccacttt ttccatttta
     3481 gcatttttac gcacgccatc acgataggct ttcaattccg tttccacata gcctgcgtgc
     3541 tgaccgctga tgcgtggata ctcgttaggc atgcccgcac catttgggcc atgacaagac
     3601 atacaggccg gtactttgcg ctcggcaatg ccaccgcgga agattttagc gcctaactca
     3661 ggattgttct taggattcac ctcacccgct tttgggtatt gcttggagaa atacaaagcc
     3721 gcatctttga ggtcttggtc tgaaaccgca cccatttggt cacgcatttc cttggcattg
     3781 cctgtggtgc gtttgccatc gcggattaat ttggcattgt gttcaatgta aaaagcattt
     3841 tgacccgcca agcgagggta agtggcgatg ccactgttgc catccaccgc atgacacgca
     3901 gcacacacat tggtggccac ttgtttaccg cgttccaaat tggccgcagg gcccgcagcc
     3961 ccagcccatg ccgaaaacag caatgttact gccacccaac tcaaacgttt catgaagtgc
     4021 tccttagctt caggcgctcc ccgttatcag cgccttgttt ttacagtcgc ttgatgcgac
     4081 tcagattatc aatcaggcaa aacgtggtat gcttacgcac cagatttgag tgattaccac
     4141 tgccatgcct ttatttcaaa atgcccaatt ctttaccacc gtcaaccact taaaagactt
     4201 accccccacc caagctgaaa tcgcatttgt gggccgctcc aatgcgggca aatccagcgc
     4261 catcaatacc ttaaccaacc atgtgcgttt ggcttacgtc tccaaaaccc caggccgtac
     4321 ccaacacatc aattttttcg agttgcgcaa cggccattac ttggccgact tgccaggcta
     4381 cggctatgcg caagtgcctg aagatgtgcg cgcacactgg gtgcgtttat tgggccaata
     4441 cctgcaacaa cgccaaagct tgataggctt gatcttaatc atggactgcc gtcatccgct
     4501 gaaagaactg gacaaacaga tgcttgattt ttttgcggta actggccacc cagtgcatat
     4561 tttactttct aaagcagaca aattatccaa gaatgaccaa atcaaaacct tgtcatcggt
     4621 caagaaagca ttaaaacctt atatgga
//
LOCUS       DAOMVK010000141         4637 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_5271_length_4637_cov_2.432562, whole genome shotgun sequence.
ACCESSION   DAOMVK010000141 DAOMVK010000000
VERSION     DAOMVK010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5271_length_4637_cov_2.432562"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="PK856_05885"
     CDS             <1..700
                     /locus_tag="PK856_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRL01934.1"
                     /translation="PVSMSLGLWTFFLTYLICIPLGIAKAVRDGSRFDGISSLLILIG
                     YAIPGFVMGILLLILFGGGSFWAVFPLGGMVSDDWDSYSSTQKIGDYLWHMVLPITAS
                     VVGNFAVMTLLTKNVFLEEIRRQYVYTARAKGLSERRIMWKHVFRNAMIPLITGFPAA
                     FLAAFFTGSLLIETLFSLDGLGLLSYEAVMKRDYPVVMGTLYVFTLMGLLAKLLSDVS
                     YVLVDPRIHFDGER"
     gene            702..1718
                     /locus_tag="PK856_05890"
     CDS             702..1718
                     /locus_tag="PK856_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRL01935.1"
                     /translation="MKWTQSRAWRQFKRHKRGYISLKIFLCLFVVSLLAPLWSNDQPL
                     WVSYQGKHYQPIFKTHMETEFGGNFDTPTDYLDPYIKKQLSRDGNWALYPINPYAANT
                     LNDFDLVPFPAPPDRKHWLGTDDRGRDVLARLVYGFRDSVLFGLVLTIITTIIGVVLG
                     AIQGYFGGKTDLFMQRFMEVWGGLPELYMLIILASFFEPSLLILLVLLSVFSWMDTNT
                     YVRAEFLKNRQMDYVQVARAMGASHRQVMWRHILPNSLTPVIALLPFKISGAILALTS
                     LDFLGLGVPSSKPSLGELLSQAKDNLDAWWIGVPTFFTLVGLLVLLIFIGEALRAALD
                     VRRQ"
     gene            complement(1791..2336)
                     /locus_tag="PK856_05895"
     CDS             complement(1791..2336)
                     /locus_tag="PK856_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01936.1"
                     /translation="MLKALNDFLLMMDATLPSNQNIRFYLSGGMLVYLYTGCQRTFYI
                     EGEFNRKVYIADGLSLVYRDGGDKERLIRFNKNDNTRFNLMHPNYLQDAVLLPFKQLQ
                     NMQVHALTALDLCVSKISRLSPIDIEDIQGFVRAGYFSAEELDVRAREALIDYFGDLK
                     QYRDNISLAKQYAQAAQSALS"
     gene            complement(2737..4086)
                     /locus_tag="PK856_05900"
     CDS             complement(2737..4086)
                     /locus_tag="PK856_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HRL01937.1"
                     /translation="MKPSTTPVQDSIPAQPAPNELFTILKILFFSGIGIFLFFVPVSI
                     NGKETILLDHATTLLTGPLKPAAIAILLLLMAYGAVAPFISGKYKDSVSNMIFSAAKV
                     MGLILALMYLTNTAPAWAMEKDMLPFLFEKLALNVGILIPIGAVALTFLIGFGLLELL
                     GVMMERLMRPLFRTPGASSIDAVASFVGSYSVGLLITDRLYTQGRYSLREAMIIATGF
                     STVSATFMIIVAKTLGIMDYWNFYFWSSLVVTFAVTTITAYMPPLSLYNNDAANPMPE
                     APKGQAWAFAKAAGINQYQSNPSILKMMKDNLMDGIRMSSVVAPSILAIGFIGLMLSK
                     FTPVFEWLGVVLKPFMWLTGIDGLSQYSGQFASGLAEMFLPAILLKDADLAVRYIAAV
                     TSISSVLFFSGSIPCMLATRIPIRFWHLVVVWLVRTVLSILLASVALRVGLAMGWLA"
BASE COUNT         1190 a         1016 c         1174 g         1257 t
ORIGIN      
        1 gcctgtgtcc atgagcttgg gtttgtggac gttttttttg acctatctga tttgtattcc
       61 cttgggcatc gccaaagccg tgcgcgatgg cagccgtttt gatggcatca gcagcttgtt
      121 gattttgata ggctatgcca tacctgggtt tgtgatgggc atcttgctgt tgattttgtt
      181 tggcggtggc agcttttggg cggtgtttcc cttgggtggc atggtcagcg atgattggga
      241 ctcttacagt agcacgcaga aaatcggcga ttatctgtgg cacatggtct tgccaattac
      301 cgcttcggtg gtgggcaatt ttgcggtgat gacgctgctc accaaaaacg tgttcttaga
      361 ggaaattcgc cgccaatacg tgtacaccgc ccgcgccaaa ggcttgagtg agcgccgtat
      421 catgtggaaa cacgtgtttc gcaatgcgat gataccgttg attacgggct ttcctgccgc
      481 attcttggcg gcttttttta caggcagcct gttgatagaa acgctgtttt ctttggatgg
      541 tttgggcttg ttgtcgtatg aagcggtgat gaaacgcgat taccctgtgg tgatgggcac
      601 gctgtatgtg tttaccttga tgggtttgct ggccaagctt ttgtctgatg tgtcgtatgt
      661 gttggtggat ccacgcatcc attttgatgg ggagcgctga gatgaaatgg acacaatccc
      721 gcgcttggcg gcaattcaaa cgccacaaac gtggctacat cagcctgaag atatttctgt
      781 gtttgtttgt ggtgtcgctg ttggcaccgc tgtggagcaa cgaccaacct ttgtgggtgt
      841 cgtaccaagg caagcactat cagcctattt tcaaaaccca tatggaaacc gaatttggcg
      901 gtaattttga cacgcctacc gattacctcg atccctacat caaaaaacaa ttgagccgag
      961 atggcaattg ggcgctgtat cccatcaatc catacgctgc caatacctta aacgattttg
     1021 atttggtgcc atttcctgca ccgccagacc gtaagcattg gttgggcacc gacgaccgtg
     1081 gtcgcgacgt gttggcgcgt ttggtgtatg gctttcgcga ttcggtgttg tttggcttgg
     1141 tattgaccat cattaccacc atcataggtg tggtcttggg cgcgatacaa ggctatttcg
     1201 gcggcaaaac cgacttgttt atgcagcgct ttatggaagt ttggggtggt ttgcctgagc
     1261 tgtatatgct gattattttg gcctcgtttt ttgagcccag cttgttgata ttgctggtgt
     1321 tgctgtcggt attcagttgg atggacacca atacctatgt gcgggccgag tttttgaaaa
     1381 accgccaaat ggactatgtg caagtggcgc gggcgatggg cgccagccac agacaggtga
     1441 tgtggcgaca catactgccc aacagtttga cccctgtcat cgccttgttg ccgtttaaga
     1501 tttcgggggc gatattggcc ttaaccagtt tggacttctt gggcttgggt gtgcccagca
     1561 gcaaacccag cttgggcgag ttgctcagcc aagccaaaga caatttggat gcttggtgga
     1621 ttggtgtgcc gacgtttttt accttggttg gcttgttggt attgttaatc tttattggtg
     1681 aagccttgcg cgcggctttg gatgtgcgca gacaatagat ctcagataga taagttgata
     1741 agaataagcg gcctgtaaac atttacaggc cgcttaattg tatgactgca ttatgataag
     1801 gctgattggg ccgcttgggc gtattgcttg gccaagctga tgttgtcgcg gtattgtttc
     1861 aaatccccaa aatagtctat caaagcctca cgcgcgcgca cgtccaattc ttccgcgctg
     1921 aaatagcctg cgcgcacaaa accttgtatg tcttctatgt ctatggggga caaacgtgaa
     1981 attttgctga cgcacaaatc caaagccgtc aacgcatgga cttgcatgtt ttgtaattgc
     2041 ttgaatggca gcaaaacagc gtcttgcaaa tagtttgggt gcatcagatt aaaacgggta
     2101 ttgtcatttt tattgaagcg gatcaagcgt tctttgtcgc ctccatcgcg gtaaaccaaa
     2161 gacaaaccat cggcaatgta gactttgcga ttgaattcgc cttcaatgta aaacgtgcgt
     2221 tggcagccag tgtacaaata caccagcatc ccacccgaca aataaaaacg gatgttttga
     2281 ttggaaggca aggtggcatc catcatcagc aagaaatcat tcaatgcttt taacatggga
     2341 tcggcagggt tgatatgttc aaaaatctta ggcattgctg gatacaaacc ccattttaga
     2401 atacttatga ttacatcata cagtgtaatg taaattgaaa catcgtttat gtaaatgcta
     2461 cctgcgaatt cttagcctgt cagttgtctt tctgctcaaa acggacacta tttgagcata
     2521 agcctgcatt aatcatcgcg attttgaatg aaaacaatca ggaaaaacag gatttggcca
     2581 ttgttcgcca tgttatagaa cgtgagcttg gctgacaata tgacaatgtt ggttggtcaa
     2641 agtactggtt tgtatacttg tttccatgag ttggcatagg cattatgaaa tgcggcctgt
     2701 agacatttac aggccgcata gaattgataa gatgatttat gccaaccagc ccatggctaa
     2761 acccacgcgc aaggcgacgc tagccaacaa gatgctcaac accgtgcgca ccaaccacac
     2821 caccaccaaa tgccagaaac gaatcggaat gcgggtggcc aacatgcacg gaatgctgcc
     2881 tgagaaaaac aacacgctgc tgatgctggt gacagcggcg atgtagcgca ccgccaaatc
     2941 ggcgtctttc aataaaatcg ccggcaagaa catttcggcc aaacctgagg cgaattggcc
     3001 gctgtattgg ctcaagccat caatgcctgt gagccacata aagggcttta acaccacgcc
     3061 caaccattca aacactgggg tgaacttaga caacatcaag ccaataaaac cgatggccaa
     3121 aatagatggc gccaccaccg aagacatgcg gatgccatcc atcaaattgt ctttcatcat
     3181 ctttaaaatg gatgggttgc tttggtattg gttaatgcct gcggctttgg caaacgccca
     3241 cgcttggcct ttaggcgctt ctggcatggg attggcggca tcattgttgt acaggctcag
     3301 aggcggcatg taagcggtga tggtggtcac ggcaaacgtc acaaccaacg aactccaaaa
     3361 atagaaattc caatagtcca tgatgcccaa ggttttggcg acgatgatca tgaaagtggc
     3421 agacacggta gaaaaacccg tggcaataat catggcttca cgcaaagaat agcggccttg
     3481 ggtgtacaag cggtcggtaa tcaacaaacc gactgaatag ctgcccacaa acgaggccac
     3541 cgcatcaatt gaagatgctc caggagtacg gaacagcggt cgcatcaatc gttccatcat
     3601 cacacccaat aattccaata aaccaaagcc gatcaaaaat gtcagtgcca cagcgccgat
     3661 gggaatcaag atgccgacat tcaaggccag tttttcaaac aaaaacggca acatgtcttt
     3721 ttccatcgcc caagcaggcg cggtattggt caaatacatc aaagccagaa tcaaacccat
     3781 gactttggcg gcgctgaaga tcatgttgct gaccgaatct ttgtatttac ctgagataaa
     3841 tggcgcaact gctccataag ccatgagcag cagcaagatt gcgatggcag caggtttgag
     3901 cgggccagtg agcaaagtcg tggcgtgatc aagcaagatg gtctctttgc cattgatgct
     3961 cacaggcacg aaaaacagaa aaatgccgat gccgctgaaa aacaggattt ttaagatggt
     4021 gaagagctca ttgggtgcag gttgagcggg gatgctgtct tgcacaggtg tggtgctggg
     4081 tttcattctc agtcctcttc gtagctttgt attatgttgt ctatacaata tcaaacaaat
     4141 tttattctgg cgaatggttt tacgcttaaa ttagagtttt tcttaagttt ctggtcgaaa
     4201 gaatatgatg ctattcacag tacttggcct gtaaataaaa gccattcaag atgctgttaa
     4261 tccgagagtt ttttaggaat gagagtgtat gtctacgatt tgggtgagat gcctatctct
     4321 gaatagaata caaagtgagt aaaagtgaag caatagaatc tgatgatggc atcgaaattt
     4381 tatggcatta ccgcaaagtg actgtatgga ttgagtgatg attgtctctt taaaagcggc
     4441 ctgtaaacac aaataaggtt tacaggccgc ttttatgtgt gcataacctc aagcctgact
     4501 gagtgtatgc atcacattgg acatgcatta cttgagcgtt ttggcaaact cagcacgcgc
     4561 ggccacggcg tggtcggtga aatcgctgaa cacgccgtcc acacccagtt tgaagtattg
     4621 ttggtattca atagatg
//
LOCUS       DAOMVK010000142         4585 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_5359_length_4585_cov_3.211479, whole genome shotgun sequence.
ACCESSION   DAOMVK010000142 DAOMVK010000000
VERSION     DAOMVK010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5359_length_4585_cov_3.211479"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            243..>966
                     /locus_tag="PK856_05905"
     CDS             243..>966
                     /locus_tag="PK856_05905"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRL01938.1"
                     /translation="MVQQTVQIPHPQGQPLGGTLFHADNITHNSAILICGGTGFLQKY
                     YAPLAIWLADKGYTVLTFDYQGIGASLHSPLKQCQVRLQDWGTHDIPAALDFLLGYTG
                     LSQVVLLGHSAGGQMLGVMDNHAKVSKVVAIAGSTGHVANMRPEFARKVKFMFGLYMP
                     LNNALFGYTKLKRIKWGEDLPRHVARQWAQWCTNGGYVQTAIDRGDIKVNYHADITQP
                     ITVIHADDDDIANEANVAEFLSL"
     assembly_gap    967..976
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1496..2929
                     /gene="cls"
                     /locus_tag="PK856_05910"
     CDS             1496..2929
                     /gene="cls"
                     /locus_tag="PK856_05910"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HRL01939.1"
                     /translation="MSAHLIWLSLLFLLHLVVIARVLLLETRSPYSRAAWVMLLLLLP
                     IIGVVLYVFFGEPWVARRTRNVARHDFQALKQQAHADSSAITQIPARYQNAFKTCASI
                     NGFPVLAGNQASLTADSNASIDAMVSDFAVAKSSIHISFYIWLTDHNGQKIIHAVQEA
                     ARRGVTCRIVVDAIGSRSLIASPHWLAMQQAGVKVAVSMKHRFGGNPLHSSRIDLRNH
                     RKIVVVDDAITYCGSQNCADPEFLVKAKYAPWVDIMLRFTGPIATQNQMLFADDWMVE
                     SGESLPADELAYAPAHPDHSFPAVAFGTGPMSAQDSMSAAFRSVIDCAQRELVISTPY
                     FVPDHPLQAALIACSRRGVAVTLIVPARNDSWFVGTMSRAYYAPLIDAGVNIHEYHGG
                     LLHAKTMVIDSEVALIGSANMDRRSLELNFENNILLYSTATASLIRERQNSYLAQASA
                     VSADDLQQRPAWKIMLQNFLTIFEPIF"
     gene            3035..3925
                     /locus_tag="PK856_05915"
     CDS             3035..3925
                     /locus_tag="PK856_05915"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRL01940.1"
                     /translation="MIDDIRYLLVFARIVETGSVSGAATALQLSAATVSAHLGKLEHS
                     MGTALLYRNTRKLSLTPAGAKLYTSAQTMQQLYADEVLAFKHEHQASEQHLRIGLPSV
                     LIHHTAFHARLSDFIRQRPQAQIELFYSDERSDLLDEHLDVVLRIGALPDSGYKARHL
                     FALERVLVTHPSLLAPSDLPTHPLQLTAYPWVALHMLPQQRVFQHAISQERVSVDFKA
                     ALQVDNVSAAYQLAHAGLGLYTPPRFMCELALAQGELVALLPDWELDAMQVCAMWHSN
                     VSPHSIAYQFVHCFEGLQAA"
     gene            complement(3922..>4585)
                     /locus_tag="PK856_05920"
     CDS             complement(3922..>4585)
                     /locus_tag="PK856_05920"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HRL01941.1"
                     /translation="QTRWHMHYSIWMAMFGFALSMSISPGPVNMLIIATAAQWGTQRA
                     WAVVSGATIGFTLLLAVVGWGLAGLLQRHPLWLQAMGVVGALFIVFMASKLISSPVSQ
                     SDAEKNITEQITQRTAMAETAPGFVHGFLLQWLNPKAWIAAAAGTALFAQAAVPHTLW
                     TFIGIYFVVCYVSLALWAWAGGVLSPFLQQGQRMRGFNVLMGVLLCLTAVFMLWQQFF
                     SN"
BASE COUNT         1089 a         1360 c         1092 g         1034 t
ORIGIN      
        1 tcaccgacac ctcgttttac cgcaataaaa actaccacac cgaacacgac accttagaca
       61 agctcgacat gaacaaggcc gcagccgtgg tcaatggcgt ggcgacatgg ttacaacagg
      121 gcgtgcatta agcctgatgc ggcctgtaaa cgaattggag tgtttcatca cattgcgttt
      181 gcctgagttg caacaagcta tgttagagtt ctcatcaagt tcacataccc gcagaggcaa
      241 acatggtgca acaaacggtg caaattcccc atcctcaagg ccagccactg ggcggcacct
      301 tgtttcatgc cgacaacatc acccacaaca gcgccatcct catttgcggc ggcacaggct
      361 tcttacaaaa atactatgcg ccactggcga tatggctggc tgacaaaggc tataccgttc
      421 tcacctttga ttaccaaggc ataggtgcct cacttcattc cccactcaag caatgccaag
      481 tccgcttaca agactggggc acacacgaca ttcccgccgc tttggatttt ttactgggct
      541 atacaggttt gtcgcaagtg gtgttgcttg gccatagcgc gggcgggcaa atgctggggg
      601 tgatggacaa ccatgccaaa gtttcaaaag tggtggccat tgcaggctcg actggtcatg
      661 tggccaatat gcggcctgaa tttgcccgca aggtgaagtt tatgtttggc ctttacatgc
      721 cgcttaacaa tgcgctgttt ggctatacca agctcaagcg catcaaatgg ggcgaagact
      781 tgccgcgcca cgttgcccgc caatgggcac agtggtgtac caatggtggt tatgtccaaa
      841 ccgccataga ccgtggcgac atcaaggtca attaccatgc cgatatcacc cagcccatca
      901 ctgtgattca tgccgacgat gatgacatcg ccaatgaagc caatgtggcc gaatttttaa
      961 gcctctnnnn nnnnnnctac cggtgcgccc acaaaaacac ctacacctta aaccccaaag
     1021 aacatggctt tcacagcata ggccacaacc tgatattccg ccccagccac caagccttgt
     1081 gggacattgt gttgcgtgaa atacaattgt tatgaaacag cgtatcacct gcctcatcat
     1141 aacaagcggc ctgtaaggtt ttcaggccgc ttgttggtgc ccacctgctt acacgtgtgt
     1201 tttgatgcgt acacttgcat cagcttggta catctattta agcaacaccc cccaacagac
     1261 ggctctttct tgttacttct tccacagtgt taaagtcaga cctgataacg agcgcgactt
     1321 gggctactaa aacccacaaa ccatgcccag acatcttacc catccattgc cactccgaac
     1381 tagccagtcg ctacgcatac ctcgtcttgt ttggcgttct cacacacaac caagcaattg
     1441 ctttaaactg tgacctcatc actgcccaat ccagcatctt tcactgagga cacccatgtc
     1501 agcacatttg atttggttaa gtttgctttt tttactgcac ttggtcgtca tcgcccgcgt
     1561 gctgctgctg gaaacccgca gcccttattc acgcgccgca tgggtgatgt tattgctgtt
     1621 attgcccatc attggggtgg tgttgtatgt gtttttcggc gaaccgtggg tggccagacg
     1681 cacgcgcaat gtcgcacgcc acgattttca ggctttgaaa caacaagccc atgccgacag
     1741 ctcggccatc actcaaattc ccgcgcgcta ccaaaatgcc ttcaaaacct gcgccagcat
     1801 caatggtttt cccgtgttgg caggcaacca agcctcgctc actgccgact ccaatgccag
     1861 catagacgcg atggtcagcg atttcgccgt ggccaaatcc agcatccata tttcgtttta
     1921 catctggctt accgaccaca atggtcaaaa aatcatacac gccgtacaag aagccgcccg
     1981 ccgtggcgtg acttgccgca tagtcgtgga tgccatcggc tcgcgctccc tcattgcctc
     2041 accgcattgg ctagccatgc agcaagctgg ggtgaaagtg gcggtatcga tgaagcaccg
     2101 tttcggcggc aaccctttac actccagccg cattgatttg cgcaaccacc gcaaaatcgt
     2161 ggtggtagac gatgccatca cttattgcgg cagccaaaac tgcgccgacc ccgaattctt
     2221 ggtgaaagcc aaatacgcgc catgggtaga catcatgttg cgcttcactg gccccattgc
     2281 cacccaaaac caaatgctgt ttgctgatga ttggatggtg gaaagcggtg agtctttgcc
     2341 tgccgatgaa ctggcttatg ccccagccca ccctgaccac agcttccctg ccgtggcttt
     2401 tggcacgggc cccatgtccg cgcaagacag tatgtcggca gcgtttcgca gcgtcatcga
     2461 ttgcgcccag cgcgaattgg tgatttccac accgtatttt gtgcccgacc atcctttaca
     2521 ggccgcttta atcgcttgta gccgccgcgg tgtggccgtg actttgattg tgcctgcgcg
     2581 caacgactcg tggtttgtcg gcaccatgag ccgcgcttat tacgcgcctt taatcgatgc
     2641 gggcgtgaac atccatgaat accacggcgg cttgctgcac gccaaaacca tggtcatcga
     2701 cagcgaagtg gccttaatcg gctcggccaa tatggacagg cgcagcttgg aattgaattt
     2761 tgaaaacaac atactgctgt actccaccgc caccgcctcg ttgatacgcg aacgccaaaa
     2821 cagctatttg gcacaagcca gcgcagtgag cgccgatgat ttgcaacaaa gacccgcatg
     2881 gaaaatcatg ttgcaaaact tcctcaccat ttttgaacca attttttaaa ccccattatc
     2941 cacaatacac gcaagcggcc tgtaaacact ttacaggccg cttatctctt tatttgcgct
     3001 atgatgtgac cacttttcta tttgctcgat acacatgata gacgacatcc gttacttact
     3061 ggtttttgcc cgcatcgtcg aaacaggctc ggtatcgggc gcggcaacgg cactgcaatt
     3121 gagcgctgcc accgtgagcg cgcacttggg caagctagaa cacagcatgg gcacggcttt
     3181 gctctatcgc aacacgcgca aactcagcct cacgcccgca ggcgccaagc tctacaccag
     3241 cgcacaaacc atgcagcaat tgtatgccga tgaggtgttg gcgtttaaac acgaacacca
     3301 agccagcgaa caacacttgc gcatcggttt gcccagtgtg ctcatccacc acaccgcttt
     3361 ccatgcccgc ctgagcgact ttattcgcca gcgcccacag gcgcagattg aattgtttta
     3421 cagcgatgag cgcagcgatt tgctggacga gcatttggac gtggtgttgc gcatcggtgc
     3481 attgcccgac agcggctaca aagcgcggca tctgtttgcc ttagaacggg tattggtgac
     3541 gcaccccagc ttgcttgcgc catcagacct gcccacccat ccgctgcaat tgacggctta
     3601 tccgtgggta gccttgcaca tgttgccgca gcaacgcgtg tttcaacacg ccatcagcca
     3661 agagcgcgtg agtgtggatt tcaaagcggc cttgcaagtg gacaatgtga gcgccgccta
     3721 ccaattggcc cacgctggct tggggctgta cacgccgccg cgcttcatgt gtgaactggc
     3781 tttggcacaa ggcgaattgg tggcgctgtt gcccgattgg gaattagacg ccatgcaggt
     3841 gtgtgccatg tggcacagca atgtcagccc tcacagcatc gcctaccagt ttgtgcattg
     3901 ttttgaaggt ttacaggccg cttaattact gaaaaactgt tgccacagca tgaatactgc
     3961 cgtcaaacac agcaacacgc ccatcaacac attgaagcca cgcatgcgct ggccttgttg
     4021 caaaaacggc gataacaccc cgcctgccca agcccacaaa gccaatgaga cgtaacacac
     4081 aacaaaataa atgccgataa acgtccacaa agtgtgtggc acagcggctt gggcaaacaa
     4141 agccgtgccc gcggcggcgg caatccaagc cttagggttc agccattgca gcagaaagcc
     4201 atgcacaaag ccaggtgcag tctcagccat ggcggttcgc tgagttattt gctcagttat
     4261 gttcttttct gcatcagact gcgacacagg actgctgatg agtttgctcg ccatgaacac
     4321 aatgaacagc gcccccacca cgcccatggc ttgcagccac aaaggatggc gttgcagcaa
     4381 gcctgccaaa ccccagccca ccaccgccag caacagcgta aaccctatgg tcgcccccga
     4441 caccactgcc catgcccgtt gcgtgcccca ttgcgccgcc gtggcgatga tgagcatgtt
     4501 cacaggccca ggggaaatcg acatgctcag tgcaaagcca aacatcgcca tccaaatgct
     4561 gtaatgcatg tgccacctcg tttgt
//
LOCUS       DAOMVK010000143         4556 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_5407_length_4556_cov_3.125528, whole genome shotgun sequence.
ACCESSION   DAOMVK010000143 DAOMVK010000000
VERSION     DAOMVK010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4556
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5407_length_4556_cov_3.125528"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PK856_05925"
     CDS             complement(<1..203)
                     /locus_tag="PK856_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01942.1"
                     /translation="MRLLLNACVLGMCLLSTSCTAILWNKGLNGPVVRHDRKEVVIVK
                     DEIASFAQLKTADAANKFLIVGK"
     assembly_gap    339..348
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(799..2016)
                     /locus_tag="PK856_05930"
     CDS             complement(799..2016)
                     /locus_tag="PK856_05930"
                     /inference="COORDINATES: protein motif:HMM:NF037255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN-like domain-containing protein"
                     /protein_id="HRL01943.1"
                     /translation="MKTVFREFYNAKFSDWDEILEHPKTLYVFDTNVLLSLYETHTNT
                     TEMLLKSIELIKSQTWMPYQIGLEFQRNRLSTLNKSNDAISEKIKKVKEAYNILQAIV
                     NDQYVKPKDTNNNVFIGQLNDLNDALNAELDRRKQEIKEYLNNDPVLERLENIFVDKR
                     IGPFFTGKEIISIKDLAEKQWKQGCGIGHEDRSKQGITHYPNDVWLEDKYGDFVYVEE
                     IKRYILQQNMTESQIEYLVLVSNDNKKDFWHKVNSDKKVPQPSFKKDILDVTKIKDFL
                     FLDSHDFLKKFKDKVTGFSDVQKQEALDNAKSLISNKFLSTNTYKQISNNLRMKEIQL
                     ELEDNFNNKYKELIDILEDSVDFDEFKAKAGFAEYTRDEINLFCKDFEDFFGEKVEDN
                     LFWRRFELQGILD"
     gene            complement(2146..>4556)
                     /gene="metH"
                     /locus_tag="PK856_05935"
     CDS             complement(2146..>4556)
                     /gene="metH"
                     /locus_tag="PK856_05935"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HRL01944.1"
                     /translation="VDSSRWNVIEKGLKCIQGKAIVNSISLKEGEAEFIAHAKEIKRY
                     GAATVVMAFDETGQADTYQKKIDICKRSYDILVNQVGFDPNDIIFDANIFAVATGIEE
                     HARYGLDFIEAVGWIHQNLPYAKTSGGVSNVSFSFRGNNKVREAIHAVFLYHAIKNGL
                     TMGIVNAGALEVYEEVEPELRERIEDVVLMRTPKDGGDATEHLIELAERFKGDAVAGK
                     QQEDLTWREKPVQERITHALVKGISTYIEADTEEARAAAKRPIEVIENTLMNGMNVVG
                     DLFGAGKMFLPQVVKSARVMKQAVAYLQPFIEEEKRLLGLDDQPANGTIVFATVKGDV
                     HDIGKNICAVVLRCNNYNVIDLGVMVPSQTILDAAIEHKADIVACSGLITPSLEEMSH
                     IAKEMQRQNFQIPLLVGGATTSKAHTAVKIEPNYHNNIAMHSLDASRLVGVVSQLLQE
                     DQAAVEAFKQSVRDEYVEVREKHNKKQAKLVSIEQARANKTPIDWAAYEPVKPSFLGR
                     KIIDNIDLGELVERFDWTVFFQSWELAGRFPAILTDEVVGEAATDLYENAQVMLKQIV
                     DEKWLTARAVIGFYPANGLDNDDIVFKTPAGDAEAMRWHSLRQQLPKTRGAYNSCLTD
                     YIAPESSGKTDYIGAFAVTAGIGIDEHVARFEAEGDDYSAILLKALADRFAEALAERM
                     HELVRKEYWGYAADEQLSNEDLIDEKYSGIRPAPGYPACPDHTTKADLWKLLNPDEIG
                     LTLTESYAMWPTAAVSGFYIAHPESRYFGTGKLAQDQVEDYAKRRGVSLEQAERDLAP
                     VLGY"
BASE COUNT         1126 a         1146 c          911 g         1363 t
ORIGIN      
        1 tctttgccca caatcaaaaa tttgttggct gcatcggctg ttttcaattg cgcaaagctg
       61 gcaatttcat ctttgacaat caccacttct tttctgtcgt gtcgcaccac agggccattc
      121 aatcccttgt tccacaatat cgccgtacac gaagtcgata acaaacacat ccccaacaca
      181 caagcattca gtaataagcg catctttttc tcatccctat tgttattaat acccactctg
      241 tccaccaact cagctccaag caacccaatc caaaacgcag tgcaaacagc acgcactttg
      301 tgcacatttc ccacctagca atactcaata gacattacnn nnnnnnnnag tgccaacagc
      361 acgcttttat ccatacctct cgccgtgcaa tctacaatag acattacatt ctaataatgc
      421 caacgtcaaa tttcatccac aaatcaccat ccagaactgg cttacatgct taagcatcgc
      481 tgcttcattg atggcacatt gcagcactgg agaatctgtc gctgtactac tgtggtagat
      541 aacgaagaat agccatccca cactagcgtc cgcacccaac tcgcccaccc taaagccaag
      601 ccctggccag accacaatga tgatgatttt gaacaatgcg gtagggtgtt gtgcatttgt
      661 gtgcatgatg tcaaagtgaa gtagattggt attgcttcta agtgtgcttg gacaatgaac
      721 aagcggcctg taaatggttt acaggccgct tttatattct gttatctata taaatcaaaa
      781 aataatttta catgttattt aatccagaat gccttgtaat tcaaaacgtc tccaaaacaa
      841 attatcttct accttttccc caaaaaagtc ttcaaaatct ttgcaaaaca aatttatctc
      901 atcacgggta tattctgcaa aaccagcttt tgctttaaat tcatcaaaat caactgaatc
      961 ctccaaaata tcaattagtt ctttatattt attattaaaa ttatcctcca gttctaattg
     1021 aatctctttc attctcaaat tatttgatat ttgtttatag gtattagtag ataagaactt
     1081 attgcttatt agactttttg cattatccaa agcctcctgc ttttgtacat cggagaaccc
     1141 tgtaacttta tctttaaatt ttttcaaaaa gtcatgggaa tctaaaaata agaaatcttt
     1201 aattttagta acatccaaaa tatctttctt aaaacttggt tgaggtactt tcttatccga
     1261 atttacttta tgccaaaaat cttttttatt gtcattactc accaacacta aatattcaat
     1321 ctgactttcc gtcatatttt gctgcaaaat atagcgctta atttcttcaa catacacaaa
     1381 atcaccatat ttatcctcta accacacatc atttggataa tgcgtaatcc cctgcttcga
     1441 cctgtcttca tgcccaatgc cacacccttg cttccattgt ttttcagcaa ggtctttaat
     1501 cgatattatt tcttttcctg taaaaaaagg gccaatgcgt ttatctacaa aaatattctc
     1561 taatcgttct aacacagggt cgttattcag atattctttt atttcttgtt tacgcctgtc
     1621 tagttccgca ttcaacgcat cgtttaaatc attaagctgc ccaataaaaa cattattatt
     1681 tgtatctttt ggttttacat actggtcatt tacaatagcc tgcaatatgt tataagcctc
     1741 cttaactttt ttaatttttt cactgattgc atcattactt ttattcaatg tcgacaatct
     1801 gttacgttga aactccaaac ctatctgata tggcatccat gtttggcttt taatcaattc
     1861 aattgatttt aaaagcattt cagttgtatt tgtatgcgtt tcatatagtg acaacaacac
     1921 atttgtatca aaaacatata acgtttttgg atgctctaaa atttcatccc aatctgaaaa
     1981 ttttgcatta taaaattctc taaaaaccgt tttcatacat tttcccctag cttcgtaaaa
     2041 agccatcaac actcattgta aatattattt ataaagcggc ctgtaaacat ttacaggccg
     2101 cttttttcat cacttcacac ttaccattca aacaacttca aactctcaat accccaacac
     2161 gggtgccaag tcgcgttcgg cttgttccaa gctcacgccg cggcgtttgg cgtagtcttc
     2221 tacttggtct tgggccaatt tgcctgtgcc gaagtagcgt gactcggggt gggcgatgta
     2281 gaaaccgctt acggctgcgg taggccacat ggcgtaagac tcggtgaggg tgaggccgat
     2341 ttcgtctggg ttgagcagct tccacaaatc ggctttggtt gtgtggtctg ggcaggctgg
     2401 gtagcctggc gcagggcgga tgccgctgta tttttcgtct atcaaatctt cattgctgag
     2461 ctgctcatcg gcggcgtagc cccaatactc tttgcgcacc aactcgtgca tgcgctcggc
     2521 caaggcttcg gcgaagcggt cggccaaggc tttgagcaag atggcggaat aatcgtcgcc
     2581 ctcggcttca aaacgcgcca catgctcgtc tatgcctatg cctgcggtca cggcaaacgc
     2641 gccgatgtaa tcggttttgc cgctgctttc aggcgcgatg tagtcggtga gacaagagtt
     2701 gtacgcgcca cgggttttgg gcaattgttg gcgtaagctg tgccaacgca tggcttcggc
     2761 gtcgcccgct ggggttttga acacgatgtc gtcgttgtcc aatccattgg cagggtagaa
     2821 accgattacg gcgcgggcgg tgagccattt ttcgtccaca atttgcttga gcatcacttg
     2881 ggcattttca tacaaatcgg tggcggcttc gccgaccact tcatcggtca aaatcgcagg
     2941 gaagcggcct gcaagctccc agctttggaa aaacacggtc caatcaaagc gctctaccaa
     3001 ttcgcccaag tcgatgttgt cgatgatttt tcgacccaag aagctaggct tcacaggctc
     3061 gtaagcggcc caatcgattg gggttttgtt ggcacgggct tgttctatgc ttaccaattt
     3121 ggcttgtttt ttgttgtgct tctcgcgcac ttccacatac tcgtcgcgca cgctttgttt
     3181 gaaggcttcc acggcggctt ggtcttcttg caacaattgc gacaccacac ccaccaaacg
     3241 cgaggcatcc aaggaatgca tggcgatgtt gttgtggtaa ttcggctcga ttttcacggc
     3301 ggtgtgggct ttggatgtgg tcgcgccgcc caccaacaat gggatttgga agttttgacg
     3361 ctgcatttct ttggcgatgt ggctcatttc ttccaagctt ggggtaatca aacctgagca
     3421 agccacgatg tcagctttgt gctcaatagc tgcgtccaaa atggtttggc tgggcaccat
     3481 cacgcccaaa tcaatcacgt tgtaattgtt acagcgcaac accaccgcgc aaatgttttt
     3541 accaatgtcg tgcacgtcgc ctttgactgt ggcgaacacg atggtgccgt tggcgggctg
     3601 gtcatctaag ccgagcaagc gtttttcttc ttcaataaaa ggctgcaaat aggccacggc
     3661 ttgcttcatc acgcgcgccg atttcaccac ttgcggcaag aacattttgc ccgcaccaaa
     3721 caaatcaccg accacattca tgccgttcat caaagtgttt tctatcacct caatcgggcg
     3781 tttggcggcg gcacgcgctt cttcggtgtc cgcttcgatg taagtcgaaa tgcctttcac
     3841 caaggcatgg gtgatgcgct cttgcacggg cttttcgcgc caagtcaggt cttcttgctg
     3901 cttgcctgcc accgcatcgc ctttgaagcg ttccgccagt tcaatcaaat gctcggtggc
     3961 atcgccgccg tctttgggcg tgcgcatcaa caccacgtct tcgatgcgct cacgcaattc
     4021 tggctcaacc tcttcataca cttccaacgc gcctgcgttg acgatgccca tggtcaagcc
     4081 atttttaatc gcatggtata agaacaccgc atggatggct tcgcgcactt tgttgttgcc
     4141 acggaatgag aacgacacgt tggacacgcc gcccgaagtt ttggcatatg gcaggttttg
     4201 gtgtatccaa cccacggctt cgatgaagtc caaaccatag cgggcgtgct cttcaatgcc
     4261 tgtggccacg gcgaaaatat tcgcatcaaa aatgatgtcg ttcgggtcga agccgacttg
     4321 gttcaccaaa atgtcgtagc tgcgtttgca gatgtcgatt ttcttttggt aggtatcggc
     4381 ttggcctgtt tcatcaaacg ccatcaccac ggtggccgcg ccatagcgct taatttcttt
     4441 ggcgtgggcg atgaattcgg cttcaccttc tttcagtgaa atcgagttca caatcgcctt
     4501 gccttggatg catttcaagc ctttttcaat cacattccag cgcgagctgt ccacca
//
LOCUS       DAOMVK010000144         4543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_5437_length_4543_cov_3.881016, whole genome shotgun sequence.
ACCESSION   DAOMVK010000144 DAOMVK010000000
VERSION     DAOMVK010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5437_length_4543_cov_3.881016"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /gene="murJ"
                     /locus_tag="PK856_05940"
     CDS             complement(<1..1180)
                     /gene="murJ"
                     /locus_tag="PK856_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HRL01945.1"
                     /translation="MNLLKTLAKVSSMTMVSRILGFIRDAIIARMFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYRQTRSPEATQAFVQHIAGLLSFMLVIVTAIG
                     IVAAPWIIYLTAPGFATSADKFELAATMLRITFPYILLISLSSLVGSILNTYHQFSIP
                     AFTPTFLNVSFIVFSLFFTPYFDPPALALAWAVFFGGILQLAFQLPALAKLGFLKMPK
                     LDFKDAAVRRVLLNMAPAILGVSVSQVSLVINTIFASFLVAGSVSWMYYADRLMELPT
                     GVLGVALGTILLPTLSKHAANQDTVNFSALLDWGLRLCLLLTLPAAVGLGVLAFPLVA
                     TLFMYNQFSALDAVNTQWALVAYAIGLVGMIVVKVLAPGFYSRQNIKTPVKIAIFTLV
                     V"
     gene            1412..2953
                     /locus_tag="PK856_05945"
     CDS             1412..2953
                     /locus_tag="PK856_05945"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRL01946.1"
                     /translation="MIDWSDGYHTEALYTYGYYKELSPSLQKLAVLLAGYETLPSTDD
                     DVHMELGFGQGISINIHAASQRGRYIGNDFNPSHVAHAQVLAQAAHSHIELTDDSFAQ
                     LDARTDLPMCNSISLHGIWSWINDENRQHIVNLIQKHLKPGGIVYNSYNSISGHAGFL
                     PWRQMMWQHYQHQSGTALERIDHTINHFNAMFESNPSLLENNPALKAAWGRIQAADRH
                     YLLHEYFNANWDCFSLSQVNELMQTAKLQFVGTTTSNPYINHLAMDENRLAQVQAQPN
                     VLAAESWRDVLLGNTFRQDLYIKGGEELNQAAILRRLQKQRFVCTVEASQFTNFLFNP
                     QDNRQFNSDLFEPVKETLVAEAYAPKSFAQIQAAVGSKYTWQHILQVLLLKISAGHLQ
                     PCVDQDTAQTRQQCHDLNAAILGKLDDKIAGVYVASPVTGMGLTLEFIYSWCLRTWLQ
                     QPEATQNDIAENIWQCLQVHGRALVDEEGKSLNVREEHFGFLAKKLADVSSLRQLWQT
                     LNLLP"
     gene            complement(3011..3691)
                     /locus_tag="PK856_05950"
     CDS             complement(3011..3691)
                     /locus_tag="PK856_05950"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRL01947.1"
                     /translation="MLTLYIGNKNYSSWSMRPAVLLDAFGLEYTECKLEFDDFSADGV
                     FKQSIGKVSAAGKVPVLVDGDTTVWDSLAIAEYLAETFPEKTMWPLDKTARAHARAIT
                     AEMHSGFQALRSACPMNIEANLTEVGQTLWQDNEALRADVRYLEKVWDSLLADGRDFL
                     FDAFSIADAYYAPVVMRLRTYGLPVNERIQAYMARIIAHPAVEKWVQAALKEELYVPI
                     DEPYRSHR"
     gene            complement(3857..>4543)
                     /locus_tag="PK856_05955"
     CDS             complement(3857..>4543)
                     /locus_tag="PK856_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HRL01948.1"
                     /translation="DDAPWLHLQGPNLWPQAADLPHFKTTVLAYQQTLREVAIKLLRA
                     FLVALDQPENALDDLIANPSVHLLKLVRYPASSAAADAQGVGAHKDTDILTLLLQDEV
                     GGLEVETDHGWIAVPLLQDAFVINIGEILELATNGYLRANVHRVVTPQGKQDRYSIAY
                     FLAPHLNSRVPHLNLSTELKLLALGPEADPSNPMFHQLGANSFKSRLRSHVAVSKKYY
                     PQSYAQISQS"
BASE COUNT         1328 a         1149 c         1016 g         1050 t
ORIGIN      
        1 tcaccaccaa ggtgaagatg gcgattttca caggggtttt gatgttttgg cgtgagtaaa
       61 agccaggtgc caataccttc accactatca tgcccaccaa gcctatggcg taagcgacca
      121 aagcccattg ggtgtttacc gcatccaatg cgctgaattg gttgtacatg aacagcgtgg
      181 ccaccaatgg gaaggccaac acacccaagc ccacggcggc aggcaaggtg agcaacaggc
      241 acaagcgcaa gccccaatcg agtaaggcgg agaaattcac tgtgtcttgg ttggccgcat
      301 gtttggacaa ggtaggcaac aaaatcgtgc ccaaagccac gcccaacacg cctgttggca
      361 attccattaa gcggtcggcg taatacatcc acgacaccga gcctgccacc aaaaacgagg
      421 caaaaatggt gttaatcacc aaagacactt gcgacaccga cacacccaaa atcgcagggg
      481 ccatgttcag caacacgcgt ctgaccgcgg catctttaaa atccaatttg ggcattttca
      541 aaaagcccaa ttttgccaaa gctggcaatt ggaacgccaa ttgcaaaata cccccaaaga
      601 acactgccca tgccaaggcc aaagcaggcg gatcaaaata cggggtaaaa aacaaagaga
      661 acacaatgaa tgacacattc aaaaatgtcg gggtgaacgc aggtatcgaa aactggtggt
      721 aagtattcaa aatactgccc accaagctcg acaatgaaat cagcaaaatg tacggaaagg
      781 taatccgcaa catcgtcgcc gccaattcaa atttgtcagc gctggtggca aaaccaggcg
      841 cggtaaggta aataatccac ggcgcggcca caatgccgat ggcggtgaca atgaccagca
      901 tgaagctgag caaacccgca atgtgttgta cgaaagcctg tgtggcttcg ggtgaacggg
      961 tttggcggta ttcggccaat atcggcacaa aagcttgaga aaaagccccc tcggcgaaaa
     1021 tgcgacggag caaattgggg agtttaaacg cgacaaaaaa cgcgtcggtg gccatgccag
     1081 cgccaaacat gcgcgcaatg atggcatcac gaataaaccc caatattcgc gataccatgg
     1141 tcatgctact gactttggcc agggttttta ataaattcat cgtattacac gggtgagacc
     1201 ataaaggcga gagtgtacac cgaagcgtgt cctaaagtat ctaggcaggt tgatttacag
     1261 tgactttcat cagtttttac tttatgttat ttgtgtgaaa aagcgccata cttcaaaatg
     1321 ggctcttctt aaaaagcctt gctgacgctt catccttgga tttgatgcaa aatccttact
     1381 gatggaactt acaaactgtg ttggagaaac aatgatagat tggtctgatg gctaccatac
     1441 ggaagcctta tacacttatg gttattacaa agaactgagc ccgagcctac aaaagctggc
     1501 ggtattgttg gcgggttacg aaaccttacc cagcaccgat gacgatgtac acatggaatt
     1561 gggctttggc caaggcatca gcatcaacat ccacgcagcc agccaacgcg gtcgttacat
     1621 cggcaatgac ttcaatccca gccatgttgc ccatgcccaa gtcttggctc aggccgcaca
     1681 ctcccacatc gaattaaccg atgacagttt tgcccaactg gatgcccgaa ccgatttgcc
     1741 catgtgcaac agcatcagct tgcacggcat ttggagctgg attaacgatg aaaaccgcca
     1801 acacatcgtc aacctcatcc aaaaacactt gaaaccaggc ggcattgttt acaacagcta
     1861 caattccatc agcggtcatg caggcttttt gccttggcgt cagatgatgt ggcaacacta
     1921 tcagcatcaa tcaggcacag ctttggagcg catagaccac accatcaatc attttaatgc
     1981 catgtttgaa agcaaccctt cattgcttga aaacaaccca gctttgaaag cggcttgggg
     2041 acgcatccaa gcagcagacc gccattactt gctacatgaa tatttcaacg ccaactggga
     2101 ttgcttttca ctcagccaag tcaatgaatt gatgcaaacg gccaaattgc aatttgttgg
     2161 caccaccacg tccaatccct acatcaatca cttggccatg gatgaaaacc gcttggccca
     2221 agtgcaagcg caaccaaatg tattagcagc cgaatcgtgg cgtgatgtct tgcttggcaa
     2281 taccttccgc caagatttgt acatcaaagg tggggaagaa ctgaatcaag ccgccatttt
     2341 acgacgctta caaaaacagc gctttgtgtg cacggttgaa gcctcacaat tcaccaattt
     2401 cttgttcaac cctcaagaca accgacagtt caatagcgat ttgtttgagc ctgtgaaaga
     2461 aactttggtg gctgaagcct atgcacccaa atcatttgct caaatacagg ccgctgtggg
     2521 cagcaaatac acttggcaac acatcttaca agtgttgtta ttgaaaataa gcgcagggca
     2581 tttacagccg tgtgtggatc aagacacagc ccaaacccgt caacaatgcc atgatttgaa
     2641 cgccgccatt ttgggcaaat tggatgacaa aatcgctggg gtgtatgtgg ccagccccgt
     2701 caccggcatg ggtttaacct tggaatttat ttactcatgg tgtttgcgca cttggcttca
     2761 gcagcctgaa gccactcaaa acgacatcgc agagaatata tggcaatgcc tgcaagtgca
     2821 tggccgtgcc ttggtagatg aagaaggcaa aagcctgaat gtgcgtgaag aacattttgg
     2881 ctttttagca aaaaaattgg ccgatgtgag ttcgctgcgc caattgtggc aaacattgaa
     2941 tttactgcct taaatccgga tgcaaaacaa aagccctgtt ttcaaacagg gctttttaac
     3001 aagcaaacac ttaacggtgc gagcggtaag gctcatctat gggcacatac aattcttctt
     3061 ttaaagcggc ctgcacccat ttctccactg caggatgcgc gatgatacgt gccatatacg
     3121 cttgtatgcg ttcattcaca ggcaaaccat aggttcttag ccgcatcacc acaggtgcat
     3181 aataggcatc cgcaatactg aatgcgtcaa acaaaaagtc acgcccatct gccaacaatg
     3241 agtcccatac tttttctaga tagcgtacat cggcacgcaa tgcctcattg tcctgccaca
     3301 aagtttgtcc cacctcagtc aaattcgctt ctatattcat cgggcaagcg ctgcgcaaag
     3361 cctgaaaacc actgtgcatt tctgccgtaa ttgctctggc atgtgctctg gcggttttgt
     3421 ccaaaggcca catcgttttt tcagggaagg tttcggccaa atattcagca atcgccaaac
     3481 tgtcccacac tgtggtgtca ccatccacca acacgggtac tttgcctgct gcggaaacct
     3541 tacctatgct ttgcttgaac acgccatcag cactgaaatc atcaaattcc agtttgcact
     3601 cagtgtattc cagcccaaat gcgtccagta acaccgcagg ccgcatagac caagaagagt
     3661 agtttttatt cccaatatac aaagtcaaca ttattttatc tttccattaa taaagaatag
     3721 cttataaaaa aatcatgtta ataaaaacac tggcgatgaa cattgtccac acaattatcc
     3781 acagctttca gcataaatac gatgttaact gaagcagcaa tctaaaaata ccgatttagc
     3841 ctaaatatta agcacatcac gactgcgaaa tctgtgcata actttgtgga taatacttct
     3901 tagaaactgc tacatgtgaa cgcagacggc tcttaaacga gtttgcaccc agttgatgaa
     3961 acatggggtt gctcggatcg gcttcaggcc ccaaagcaag tagtttcagc tctgtggata
     4021 aattcaaatg cggtacgcgg ctgttcaaat gcggcgccaa gaagtaggcg atggagtagc
     4081 ggtcttgctt gccttgtggt gtgaccacac ggtgcacatt ggcgcgcaaa tagccattgg
     4141 tggccaattc taagatttca ccgatgttaa tcacaaaagc gtcctgtaac agcggcacag
     4201 ctatccagcc gtgatcggtt tccacttcca agccaccgac ttcgtcttgc aacaacaagg
     4261 tcaaaatgtc ggtgtctttg tgcgcgccca cgccttgtgc atcagcagcg gcactggaag
     4321 caggatagcg caccaatttc aacaaatgca cagacggatt ggcaatcaaa tcgtccaagg
     4381 cattttcagg ttgatccaag gccaccaaaa acgcgcgcaa aagcttgatg gcaacctcac
     4441 gcaaggtttg ttggtaagcc aaaaccgtgg ttttaaaatg cggcaaatca gctgcttgtg
     4501 gccacaaatt cggcccttgc aaatgcaacc aaggcgcatc gtc
//
LOCUS       DAOMVK010000145         4527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_5465_length_4527_cov_2.761181, whole genome shotgun sequence.
ACCESSION   DAOMVK010000145 DAOMVK010000000
VERSION     DAOMVK010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5465_length_4527_cov_2.761181"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..935)
                     /gene="panC"
                     /locus_tag="PK856_05960"
     CDS             complement(96..935)
                     /gene="panC"
                     /locus_tag="PK856_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRL01949.1"
                     /translation="MQIITTIKELRAWRNSVDQVAFVPTMGNLHDGHLALVQEAVAQA
                     PHVVVSIFVNRLQFGQGEDFMSYPRTLDVDAAKLEAAGVEVLFAPNEAELYPHVSQNY
                     QVEPPHLQNELCGAFRPGHFRGVATVVSKLFNIVKPNVACFGKKDYQQLAVIRGFVED
                     LNFDIRIVGVDTGRAADGLALSSRNGYLTEAERMQAPQLYQALSDMAAQIRAGRQDYA
                     NLEQAASQYLQSQGWVVDYIEVRHAGNLQIAHAGDAHVVVLAAARLGKTRLIDNLEIH
                     LEA"
     gene            complement(1016..1810)
                     /gene="panB"
                     /locus_tag="PK856_05965"
     CDS             complement(1016..1810)
                     /gene="panB"
                     /locus_tag="PK856_05965"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRL01950.1"
                     /translation="MITVSTLNKMKQTGEKIAMLTCYEASFASLMSDAGVDMLLVGDS
                     LGMTVQGHDSTLPVTLADMVYHTRAVARGNKNAMIVTDLSFGSFQQNKEQAFAAAVEL
                     MQAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVNAFGGYRVQGKGDAAAQ
                     ALLADAKAHAKAGAALVLMECVPAALAAEVTCSVACPTIGIGAGADCDGQVLVMHDML
                     GVFPGKTARFVRNFMQGQDSIQAAVSAYVEAVKKSEFPAAEHQFSA"
     gene            complement(1823..2467)
                     /locus_tag="PK856_05970"
     CDS             complement(1823..2467)
                     /locus_tag="PK856_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HRL01951.1"
                     /translation="MDYRYLVVEGSIGSGKTALSRKLAQHFECELLSEAPEQNPFLTK
                     FYLNAANHGLATQLHFLMERAAAASIVLEEDRQHQRVISDFLLEKDQIFVPIVLQDDE
                     ATLYWQIKQQVLPEYPVPDLVIYLQGKQTANHAQANGSQSAHYKLFPNGYMAQVDDEY
                     SHFFHLYTHAPLLIANTDELDFINNDEHFQLLLSTMQNMRGSRHYLNLNETTPQ"
     gene            complement(2481..2999)
                     /gene="folK"
                     /locus_tag="PK856_05975"
     CDS             complement(2481..2999)
                     /gene="folK"
                     /locus_tag="PK856_05975"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRL01952.1"
                     /translation="MMTHSALIALGSNLSEPVMQIQRAISCLSQQAGIRLQAASALYR
                     TAPVGYADQPDFINAAVHIETNLSPMVLLRVLNQIEAEFGRERSFQNAPRTLDLDLID
                     YAGQQWQTERLVLPHPRAHERGFVLIPLADMVPEHVLGAHGQVVQLLQGMDVSDIERL
                     DVLAWNGKASSN"
     gene            complement(2996..>3790)
                     /locus_tag="PK856_05980"
     CDS             complement(2996..>3790)
                     /locus_tag="PK856_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HRL01953.1"
                     /translation="REDPVRVLRALRLSTKLGFELDEEIAEVMSACSPWLGAEPPARL
                     VDELIKLLLSGHALACLHNIRRMGVDSSVHPFLQVLIEQDLDEPFIQLALENTDTRIK
                     QGKHVSIGFLLAAVLWPKVRGAWQECVASGLKSQFALNEAIEQVRMELDSSSWGLPHR
                     MTAMCREIWQMQAQFDNQRGQRPFRLLAQARFRAAYDFLLLRHEVGEENTDSVLWWQT
                     FQAVSDDERADMVQARPLPVLDEDAAAPKKRRRRRKPKTANKPKSA"
     assembly_gap    3791..3865
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3866..4277)
                     /locus_tag="PK856_05985"
     CDS             complement(<3866..4277)
                     /locus_tag="PK856_05985"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HRL01954.1"
                     /translation="MTNKRLKKAAANDGSTVPALILDGKDFGLSLSKLSNAAQKVVKQ
                     LHQSGFDAMVVGGGVRDLMVGLTPKDFDIATNATPEEVRAVFKRSRIIGRRFRIVHVM
                     VGPETIEVTTYRGGDEVMQNEQGRIMRDNTFGSQA"
BASE COUNT         1148 a         1259 c          942 g         1103 t
ORIGIN      
        1 acgggcatta cccattgtcc aacgatagaa ttttctgccc atgccaacat cggtacggtc
       61 aaacacatca cagccaatat ctgtttcaat gccatttagg cctccaaatg aatttccaaa
      121 ttatcaatca aacgtgtctt acccaaacgt gcggcagcca acaccaccac atgtgcgtca
      181 cctgcatgtg caatttgcaa attgccagca tgacgcacct caatatagtc aacaacccaa
      241 ccttggcttt gcaaatattg gctggccgct tgttctaaat ttgcatagtc ttgccgaccc
      301 gctcggattt gcgccgccat atcgctcagt gcttggtata actgtggtgc ctgcatgcgc
      361 tcagcttcgg tcaaatagcc attgcgactg gacaatgcca agccatcggc agcacggcca
      421 gtatccacac ccacaatgcg gatgtcaaaa ttcaaatcct caacaaagcc ccgtatcacc
      481 gccaactgtt ggtagtcttt tttgccaaaa cacgccacat tgggtttcac aatgttaaag
      541 agtttgctga ccaccgtcgc cacaccacga aaatgacctg ggcgaaacgc accgcacaat
      601 tcgttttgca aatgtggcgg ctccacttgg tagttttgac tcacatgcgg atacagttcg
      661 gcctcgtttg gggcaaacaa tacttccaca cctgccgcct ccaatttggc agcatccaca
      721 tccaaagtcc gaggataaga cataaagtct tcgccttgcc caaactgcaa gcggttcaca
      781 aaaatgctca ccaccacatg cggcgcttgc gccacagctt cttgtaccaa agccaaatgt
      841 ccgtcgtgca gattgcccat ggtcggcaca aacgccactt ggtcaacact gttacgccat
      901 gcgcgcaact ctttaattgt tgtgataatt tgcatcactg aaatcctatt ctggcaagca
      961 cacaggctgc caatattgga tattgacagc ctgttacgct tagatgccgc ctatattacg
     1021 cgctaaactg gtgctctgca gcaggaaatt cagatttttt taccgcctca acataggcgc
     1081 tgacagcggc ctgtatgctg tcttggcctt gcataaagtt gcgcacaaag cgggcggtct
     1141 tacctgggaa cacccccaac atatcgtgca tcaccaacac ttggccatca caatccgcac
     1201 ccgcacctat gcctatggta gggcaagcaa ctgaacacgt cacctctgca gccaaagccg
     1261 caggcacaca ttccatcaac accaatgcag cgcctgcctt ggcatgcgct tttgcatcgg
     1321 ccagcaaagc ctgcgctgcc gcatcaccct tgccttgcac cctatagccg ccaaacgcat
     1381 tcaccgattg cggggtcaag ccaatgtgag cacacacagg tataccacgc atttgcagaa
     1441 actcagtggt ttccgccatc cacaccccac cttccaattt caccatgtgc gcgcctgctt
     1501 gcatcaattc aactgctgcg gcaaatgctt gctctttgtt ttgctgaaag ctgccaaatg
     1561 acaaatcggt cacgatcatg gcattcttat taccacgcgc caccgcgcga gtgtggtaga
     1621 ccatgtcagc caacgtcaca ggcaaagtag aatcgtgacc ttgcaccgtc atgcccaatg
     1681 aatcccccac caacaacatg tcaacgcctg catcgctcat caaactggca aaactggctt
     1741 cataacaggt caacatggcg attttctcgc ctgtctgttt cattttattt aaggtgctta
     1801 cagtaatcat ggtgtgcttc ttttattgag gcgtggtttc gttcaaattc aaataatgac
     1861 gtgagccgcg catgttttgc atggtggata gcaacaattg aaaatgctcg tcattgttga
     1921 tgaaatccaa ctcatcggta ttggcaatca acaagggcgc atgggtatac aaatgaaaga
     1981 aatggctgta ttcatcgtca acctgtgcca tataaccgtt tggaaacaat ttgtaatgcg
     2041 ccgattggct accattggcc tgcgcatgat tggctgtttg tttaccttgt aagtaaatca
     2101 ccaaatcagg cactggatac tcaggcaaaa cctgttgctt aatctgccaa tacaaagtcg
     2161 cctcatcatc ttgcagcacg atgggcacaa atatttggtc tttttccagc aaaaaatcgc
     2221 tgatgacacg ctggtgttgg cggtcttctt ccaacacaat gctcgcggca gctgcgcgct
     2281 ccatcaaaaa gtgcaactgt gtggccaaac catggttggc ggcattcaaa taaaacttgg
     2341 ttaaaaatgg attttgttct ggtgcttcgc tcaacaattc acattcaaaa tgttgggcca
     2401 atttgcgact gagggcggtt ttgccactgc ctattgagcc ttcaaccacc aaatagcgat
     2461 aatccatgtt tgctcctaaa ttaattagat gaggctttgc cattccaagc caggacgtcc
     2521 aaacgttcga tgtcactgac atccatcccc tgcaacagct gcaccacctg tccatgcgct
     2581 cccaacacat gttcaggcac catgtcagcc aatggaatca atacaaaccc acgctcatga
     2641 gcgcgtggat gcggcaaaac cagacgttct gtttgccatt gctggcccgc atagtcaatc
     2701 aagtccaaat ccaaggtgcg tggcgcattt tgaaagctgc gctcacgccc aaattcggct
     2761 tctatttgat tgagcacccg caacaatacc ataggagaca agttggtttc tatgtgcact
     2821 gccgcattaa tgaaatctgg ttgatctgca taacccacag gggcagtacg gtacagtgca
     2881 gatgcggcct gtaaacgaat acccgcttgc tgagataagc aactgattgc acgttgtatt
     2941 tgcatcaccg gctcagacaa attgctgccc agggcaatca aagctgaatg cgtcatcatg
     3001 cgcttttggg tttattagcg gttttgggtt tgcgacggcg acggcgtttt ttgggcgcag
     3061 ctgcgtcttc atccaaaaca ggcagcggtc tcgcttgcac catgtcagca cgctcatcat
     3121 cagagacggc ctgaaaggtt tgccaccaca acacactgtc ggtgttttcc tcacccactt
     3181 catggcgcaa caacaagaaa tcatatgcgg cacgaaaacg tgcttgcgcc aacaaacgaa
     3241 atgggcgttg accgcgctga ttgtcaaact gcgcttgcat ttgccagatt tcacgacaca
     3301 tcgccgtcat gcgatggggc aaaccccaac tactgctgtc caactccatg cgcacttgtt
     3361 ctatggcttc gttcagggca aattggcttt tcaggccgct tgccacacac tcttgccatg
     3421 cacctctcac cttaggccac aacacagccg ccaataagaa acctatcgac acatgtttgc
     3481 cttgcttgat gcgtgtatcg gtattttcca aagccagttg aatgaaaggc tcatccaagt
     3541 cttgctctat caacacttgc aagaatggat gcactgaaga atccactccc atgcgacgga
     3601 tattgtgtaa acacgccaat gcatgccccg acagcaacag cttgattaac tcatcgacca
     3661 aacgtgcagg cggctcagcg cccaaccacg gtgaacacgc cgacatcacc tcagcgattt
     3721 cttcatccag ctcaaaaccc aatttcgttg ataaacgcaa agcacgcaat acccgcacag
     3781 ggtcttcacg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnncggct tggctgccaa acgtgttgtc acgcatgatg
     3901 cggccttgct cgttttgcat tacctcgtcg ccaccacgat aggtagtgac ttcaatggtt
     3961 tcgggcccca ccatcacatg cacaatgcga aatcgacggc caatgatgcg gctgcgcttg
     4021 aacacagcac gcacttcttc aggcgtagca ttggtggcaa tgtcaaaatc tttaggcgtt
     4081 aaacccacca tcaagtcgcg cacaccaccg ccaaccacca tggcatcgaa accactttgg
     4141 tgtaattgct tgaccacttt ttgcgcagca ttgctcaatt tagacagact caacccaaag
     4201 tcttttccat ccaaaatcaa tgcaggcacg gtgctgccat cattggcagc cgcttttttt
     4261 aaccgcttgt ttgtcataga tttcgacgcc ttacaggacg ccgctgctga ttgtatcggc
     4321 gtgcattata cgctaaatgc ctgcttttca tgcttgaaaa ctgagtttgt gcatcgccta
     4381 cctacctagc cgccttaaga ttggttataa tgcgccttta ttgataggtt cgcgttgact
     4441 ccaatgggtc gacctcatta cggcagaaca aactatgtac aactaccaat cagaattcac
     4501 ccaattcatg aatgactatc tggaaaa
//
LOCUS       DAOMVK010000146         4507 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_5492_length_4507_cov_3.521114, whole genome shotgun sequence.
ACCESSION   DAOMVK010000146 DAOMVK010000000
VERSION     DAOMVK010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5492_length_4507_cov_3.521114"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            448..1338
                     /locus_tag="PK856_05990"
     CDS             448..1338
                     /locus_tag="PK856_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRL01955.1"
                     /translation="MRWTLDQLQQFVATAESGSFSEAARQLGKAQSAVSTAIGLLEAD
                     LGVELFDRTRRSARLTNAGEILLLEAQELMNQAQSLDKRAHSLSFGQDAKLSVALDEA
                     LPYSVISQIIREIAQYFPDLELTLLNGTATEVAHYVQQGRAQIAFHFVRGALPSYFDQ
                     CHIGSVAQGVFVPNDHALTQCDSSLLKKELVKFRQLVMHTEDVNETAYSPKIWRSDSY
                     FSLAEMVADDLGWAILPVHIAHSEKHLKPLAQIDCPFMTLPPLSVRMVWFQGWELNQV
                     SHWVQTRFTELLRKVPKAEL"
     gene            1492..1872
                     /locus_tag="PK856_05995"
     CDS             1492..1872
                     /locus_tag="PK856_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01956.1"
                     /translation="MVKQVLKTLLFGMLMAMWLLGSAWRVDTAPESCTVHECSSMAFV
                     AANSVAPTSMAMHYASPIDAMSCSHSVCCTGVLGLLPLLCLLLMVCAQRAYQTLTVSF
                     VSHVKTALWRPPQTLLLSPAFYSH"
     gene            1890..2369
                     /locus_tag="PK856_06000"
     CDS             1890..2369
                     /locus_tag="PK856_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HRL01957.1"
                     /translation="MTIKNTKRLRHHAWALCASAAMAMGLTLISSSVLAQSLPPATLY
                     KSSTCGCCTEYVKYLRANGMKINALDHENMGAIKQQMGTSKAASCHTMKVGNYVVEGH
                     VPIAAIRKMLAEKPNIKGIALPGMPYNSPGMGPEKKGSLKVLSINRAGNVQGVYVTL"
     gene            complement(2467..3759)
                     /locus_tag="PK856_06005"
     CDS             complement(2467..3759)
                     /locus_tag="PK856_06005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRL01958.1"
                     /translation="MELKHKCLWPDLVAHIPTYPALQGEAKADVCVIGAGFTGLSAAI
                     RLRELGLSVRVLEGTHLAYGGSGRNVGLVNAGSWIKPDEFDKILGTEAGKKFNQAFGD
                     APQLVFDLIQQYNLDAQHTHTGTLHMAHNAKGSADIQDRYQQWTKRGANMCLLEGAEC
                     QEYCGTDKIPSALLDLRCGTVNPFAYASALASTAAQLGAHIHEQSKVTHINRQGSQWL
                     VRAENGSVLADKVIIASNAYTEGNWTDVTRAIYPGYYYQVASKPLSGAAADSILPHKQ
                     GSWDTRTVLSSIRRDQAGRLILGSLGRSDNKPAWFIKKWADRVQQFYYPQLGNNVEWE
                     YVWCGRIGFTDDHIPRVFEPERGVVAATGYNGRGVTTGTLMGRAMVDYLVQDDPAILP
                     LPFKGVNECECKNSNLKSLFYETSFSAYHLGQCLNLVR"
     gene            complement(3813..>4507)
                     /locus_tag="PK856_06010"
     CDS             complement(3813..>4507)
                     /locus_tag="PK856_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HRL01959.1"
                     /translation="LAINHCVHFSALWVEIEQLTRAGLIAINMTPSHAWVAPSGSREP
                     LLGTNPIGFGFPRRNPDEPYVFDFATSATARGEIQLHDRNGTPLPEGVAIDADGQPTT
                     DPKAAMAGAMLTFGGHKGSAISTMVELLAGIAIGDMTSQESMAFADGTPTLPYGGEIL
                     IAIDPVMLLGEDVDSLYARGDAYLNQFNAAGARLPSQRRFKARQQNTAQGYLELDDSL
                     YQDLQALLQAGL"
BASE COUNT         1038 a         1127 c         1238 g         1104 t
ORIGIN      
        1 acccacaact tccagcgcaa tcgacgctgc taaaaacaac cagtaaatca tttttctttt
       61 cctaaaactt gatggcgcaa gcccaatagg cgcgcacaaa cagagcctgc agtgttgcag
      121 acagtaaaat agcggagcgg aaaagaaaga ttaaggttcg gtttggccgc tgtactcaaa
      181 gcatgaggct tgaatgcgga gggtgaaatg gttggcgagc gagtgcaaag acgggaaaac
      241 gcggtaggcg ttagtcatgg gacgcttcat ttactgtgcc gcaggtggca cacagtagac
      301 tactttcctt tctgtatttt gaatggtgtt tgattcgagg taagttggct tgaacccatg
      361 tgggcttaaa ccgaatacaa gcattattcc atctgtaatc tgatgtataa agtttatatc
      421 catctatttt tttgataata tatcgaaatg agatggacat tagaccaatt acaacagttt
      481 gtggccacgg ctgaaagcgg ctctttcagc gaggccgcgc gccaattggg caaggcacaa
      541 tcggcagtga gcaccgccat cggcttgttg gaagccgact tgggggtgga gctgttcgac
      601 cgtacccgcc gcagtgcacg tttgaccaac gcgggtgaaa tcttgctgct cgaagcacaa
      661 gaattgatga atcaggccca gtctttagac aaacgcgccc attccctaag ctttggccaa
      721 gatgccaagc tgtcggtggc tttggacgag gccttgccgt attccgttat cagtcaaatc
      781 atccgcgaaa tcgcgcaata ctttcctgat ttggaattga cgctgctcaa tggcactgcc
      841 accgaagtgg cacactatgt gcaacaagga cgggcacaaa ttgcgtttca ctttgtgcga
      901 ggcgcactgc cttcgtattt cgaccaatgc cacatcggct cggtggcgca aggcgtgttt
      961 gtgcccaacg accatgcctt aactcagtgc gacagcagtt tgctcaaaaa agaattggtg
     1021 aagtttcgcc aattggtgat gcacaccgaa gacgtgaacg aaaccgccta cagcccgaaa
     1081 atttggcgct cagacagcta tttcagcttg gcggaaatgg tggccgatga tttgggttgg
     1141 gcgattttgc ctgtgcatat tgctcattcg gaaaaacact taaagccgct ggcgcaaatc
     1201 gattgcccgt tcatgacgct gccgcccttg tcggtgcgca tggtgtggtt tcagggctgg
     1261 gaattgaatc aggtgtcgca ttgggtgcaa acacgcttta ctgaattgct gcggaaagtg
     1321 cctaaagcgg agctttgagc ggaagattca gcctgttggc aagtttggtg cggcctgtaa
     1381 gccttacagg ccgcttgtta aagcggtgtt gatgcaccac aaagtcttaa atgtgtgcat
     1441 ctgttacatt gttgctgata caaatgtatt gaaaaagata gggaattggg tttggtcaag
     1501 caggtgttga aaacactgtt gttcggaatg ctgatggcga tgtggctatt gggctctgct
     1561 tggcgcgtgg acaccgctcc agagtcttgt acggtgcatg agtgctcatc catggcgttt
     1621 gtggcggcca acagtgtcgc ccctaccagc atggccatgc attatgccag cccaattgac
     1681 gccatgtctt gcagccattc ggtgtgttgt acgggtgtgc ttgggctgtt gccattgctg
     1741 tgtttgctgc tgatggtgtg tgcccaacgc gcgtaccaaa ccctaaccgt gtcttttgtt
     1801 tcacacgtta aaaccgcatt gtggcgcccg ccacaaaccc tcttgttgtc acctgctttt
     1861 tattcacatt aataattaag gaaaacaaga tgactatcaa aaataccaaa cgcttgcgcc
     1921 atcatgcatg ggcattgtgc gccagtgccg ccatggcgat gggcttaacc ctgatttcaa
     1981 gttcggtttt ggctcaaagt ttgccacccg ctacgctgta taaatcgtct acctgtggct
     2041 gttgtaccga gtacgtgaag tatttgcgtg ccaacggcat gaaaatcaat gcgctagacc
     2101 acgaaaacat gggtgccatc aaacaacaaa tgggcaccag caaagccgcc agttgccaca
     2161 ccatgaaagt gggcaattat gtggtggaag gccatgtgcc gattgccgcc atccgcaaga
     2221 tgttggccga gaaacccaac atcaaaggca tcgctttgcc aggcatgcct tacaattccc
     2281 caggcatggg gcctgagaaa aaaggcagcc tcaaggtatt gagcatcaat cgcgccggca
     2341 atgtgcaagg cgtgtacgtg actttgtaag cattgcaatg aaaacagcca ctgcaaagtg
     2401 gctgtttttg tatggctaag gctgaaaaag cttacaggcc ggcctgtaaa tgggcttgca
     2461 cacgctttag cgcaccaaat tcaggcattg gcctaagtgg taagcgctga atgaggtttc
     2521 gtaaaacagc gatttcaaat tgctgttttt gcattcgcac tcgttcacgc ctttgaaagg
     2581 caaaggcaaa atggcggggt cgtcttgcac caaataatcc accatcgcgc gccccatgag
     2641 cgtgcctgtg gtcacgccgc ggccgttata gcctgtggcc gccaccacgc cacgctcggg
     2701 ctcaaacacg cgcggaatgt ggtcatcggt aaagccgatg cgaccgcacc aaacgtattc
     2761 ccattccaca ttgttgccca attgcgggta gtaaaattgc tgtacgcggt cggcccattt
     2821 tttgatgaac caggccggtt tgttgtcgct gcggcccaag ctgcccaaaa tcaaacggcc
     2881 tgcttggtcg cgacggatgc tggacaacac cgtgcgcgta tcccacgagc cttgtttgtg
     2941 cggcaaaata gaatctgctg ccgcgcccga taagggtttg gacgccactt ggtagtaata
     3001 gccagggtag atggcgcggg tcacatcggt ccaattgcct tcggtatagg cattgctggc
     3061 gatgatgact ttgtctgcca acacgctgcc gttttcagca cgtaccaacc attgtgagcc
     3121 ttggcggttg atgtgggtga ctttggactg ctcatggata tgagcgccca actgggcggc
     3181 ggtgctggct aaggcgctgg cataggcaaa cggattcacc gtgccgcaac gcaaatccaa
     3241 taaggcactg ggaattttgt cggtgccgca atattcttgg cactccgcgc cttcaagcaa
     3301 gcacatgttg gcaccgcgtt tggtccattg ttggtagcgg tcttgaatgt cggcgctgcc
     3361 tttggcattg tgggccatgt gcaaagtgcc cgtgtgggtg tgttgggcat ctaagttgta
     3421 ttgctgtatc aaatcaaaca ccaattgcgg tgcatcacca aaagcctgat tgaatttctt
     3481 gcctgcttcc gtgcccagaa tcttgtcaaa ctcatcgggc ttgatccacg agcctgcgtt
     3541 aaccaagccg acattgcgcc ccgagccacc gtaagccaaa tgcgtgccct ccaaaacgcg
     3601 cacgctgagc cccaattcac gcaagcgtat ggcggccgat aggccagtga agcctgcgcc
     3661 tataacgcag acatcggctt tggcttcacc ttgtaaggcg ggataagtcg gaatgtgagc
     3721 caccaaatcg ggccacaggc atttgtgttt caattccatg gtgatgcatc ctttgtttct
     3781 cggttttatt attgattttg atgcagcaag gtttacaggc cggcctgtaa caaggcttgt
     3841 aagtcttggt acaggctgtc gtccaattcg aggtaacctt gtgcggtgtt ttgttggcgc
     3901 gctttgaagc ggcgctgtga aggcaagcgt gcgcctgcgg cgttgaattg gttcaaatac
     3961 gcatcgccgc gcgcatacaa gctgtccacg tcttcaccta acagcatcac agggtcgatg
     4021 gcgatgagga tttcaccgcc gtaaggcaag gtgggtgtgc cgtctgcaaa cgccattgat
     4081 tcttggctgg tcatgtcgcc tatggcgatg cctgcgagca attccaccat ggtggagatg
     4141 gccgagcctt tgtggccgcc aaacgtcagc atcgcgcctg ccatcgccgc tttcgggtct
     4201 gtggtgggct gaccatcggc atcaatggcc acgccttcag gcaaaggggt gccattgcgg
     4261 tcgtgcaatt ggatttcgcc acgtgcggtg gcgctggtgg caaagtcaaa cacatacggc
     4321 tcatctggat tgcggcgtgg aaagccaaag ccaatgggat tggtgcccaa taaaggttcg
     4381 cggctgcccg atggcgcgac ccatgcatgg cttggggtca tgttgatggc tatcaggcct
     4441 gcgcgggtca gttgttcgat ttctacccac aaggccgaaa aatgcacaca gtggttaatc
     4501 gccaata
//
LOCUS       DAOMVK010000147         4500 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_5505_length_4500_cov_3.191226, whole genome shotgun sequence.
ACCESSION   DAOMVK010000147 DAOMVK010000000
VERSION     DAOMVK010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5505_length_4500_cov_3.191226"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PK856_06015"
     CDS             complement(<1..286)
                     /locus_tag="PK856_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019389696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HRL01960.1"
                     /translation="MNQAFALPVPSGHATLEQYIHTVNSIPLLSAEEEHDLAQRHALE
                     DDLEAAKKLILAHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRFEP"
     gene            complement(467..1759)
                     /locus_tag="PK856_06020"
     CDS             complement(467..1759)
                     /locus_tag="PK856_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01961.1"
                     /translation="MGTPRLNTFVTPKPHAGLIRTLIPFNRLVNLKGIPLLRDIPYLN
                     RLPIVRGLCDIREIDLDDTSKALLAFAMNGSNASFITPNHPEFFTDWMLDKEIASRFY
                     PLTANWATHSIVNGMGKWGQKFWLANHLIAQVPGDTEAALTYSKQAAASGTPVLLHPE
                     GQVLWQSDYVNPLFHGAAQMAMELAQERPEKPVLIQPVVWKLRFIRNVSRELMQELAY
                     VEQQLGLPQQPHLALPLRLAKVHRDVLTQTFTYLGYIAPKGRNYFDLRDALLSRINQD
                     LQTRFGAFTGDSEEQSRQLLRTIRQEEKQGKPLDTMTVRKRRELQRLLRTPKTAYSAE
                     RWSQENIAECIKRLRNDYLKKGWRNALHHFVPRPVAGRIAHIRACESIDVQAWLQQHP
                     DTSADSIMQEVRQRMQNALDELKTQFPATQTYANPFWL"
     gene            complement(1827..3368)
                     /gene="lnt"
                     /locus_tag="PK856_06025"
     CDS             complement(1827..3368)
                     /gene="lnt"
                     /locus_tag="PK856_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HRL01962.1"
                     /translation="MVNIQRLFARTSPVWGWFVMVVSALLTPFAYAPYRLYWLMPILV
                     AAMLVIAVKYQAYRLRMVYVWALIAYFVQCYWINTALHDIAGLPSYISIPLTALLPAY
                     LALYPTLAIWLCEKIRLPVVWRLLLVFPLAWTLTEFIRERALTGFGWGAIGYSQIAES
                     PLAAYAPISGIALVTWATAVVSAALAALILCKPVWQKAVHLISAIVVIGIGLLLGQQR
                     WTQADGSTATVALLQGNIEQSMKWDPEAFQHTIQTYYDMVAETSADIVILPETAIPVM
                     RQDLPDGLLEQFASTAKRNNAALAIGIPQYTPTGRQYLNSVINLSEFNPEQTDKPLPS
                     YSKDHLVPFGEFKPIFTDWLYRMMHMPLADFSEGGNNQAPLQLANQQIAFNICYEDSF
                     GDDLIASAKTSTLLSNVSNMAWYGTSHAMDLQLQQSQARALELGRYMVRSTNTGLTAV
                     LDPQGHITALAPRDTKQVLTVTIQGYQGLTPYMQLGSTWPLAIVFTVLLLLLYGYSRR
                     KPTQQ"
     gene            complement(3368..4267)
                     /locus_tag="PK856_06030"
     CDS             complement(3368..4267)
                     /locus_tag="PK856_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HRL01963.1"
                     /translation="MAGSLLLLLDDIAAVLDDVSVMTKVAAKKTAGVLGDDLALNAEQ
                     VSGSASNRELPIVWAVAKGSFWNKVILVPLFLALSYFLPFMIGPVLIVGGAFLAFEGV
                     EKVLHSIHKMRHKHDATHSADTVADVAFDEKGKIKGAIRTDFILSAEIIAISLGSMSH
                     EPILKQAIALAIISIVFTAGVYGLVAGIVKMDDVGFWLKQKSSAVAQKFGAFLINSTP
                     YLMRFLTIAGTAAMFLVGGSIIVHNIPFLAHISHELDSQFTGIVKFLVVHGLDVLVGA
                     IVGGIIVWIVNMVNSLRGTKAAH"
     gene            complement(4295..>4500)
                     /locus_tag="PK856_06035"
     CDS             complement(4295..>4500)
                     /locus_tag="PK856_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HRL01964.1"
                     /translation="TEIEDFNEYFKTDYSDEEADTIGGLVIHEFGRLPQRGEKVAIDK
                     WLFTIARADARRLHVLLVTPNPS"
BASE COUNT         1150 a         1070 c         1159 g         1121 t
ORIGIN      
        1 ttggctcaaa acgttttacg gctttcatca ggccgatatt gccctcttga attaagtcgg
       61 cttggttcaa accatagcca tcataaccac gggcaatcga taccaccacg cgcaagtgtg
      121 ccaaaatcaa ttttttggcc gcttccaaat cgtcttctaa agcgtggcgt tgagccaaat
      181 catgctcttc ctcagcactc aacaacggaa tgctgttcac agtgtgaatg tactgctcca
      241 aagtggcatg accgcttggt acaggcaaag caaaagcttg attcatcaat ttttgtcctt
      301 atcttaatcg tgtgacaaat tcacccttct gagtttagca taaaacacgc caattctcgg
      361 gcttgtcaca taaatttaac ttttattaag acacttagca agttcaatag tttcattaaa
      421 ttaagtaaaa tcttaagtat attttattgt aataaaattc aatccgttac aaccaaaatg
      481 ggttggcata ggtttgcgtg gcaggaaatt gggtttttaa ttcatctagc gcattttgca
      541 tgcgctgtct tacttcttgc atgatgctgt cggcactggt atcagggtgt tgctgcaacc
      601 aagcttgcac atcaatggac tcacacgcac gaatatgggc gatacgaccc gccacagggc
      661 ggggcacgaa atggtgcaag gcattgcgcc agcctttttt caaatagtcg ttgcgcaaac
      721 gtttgatgca ttcggcaatg ttttcttggc tccagcgctc ggctgaatac gcggttttgg
      781 gtgtgcgcag taagcgctgt aattcgcggc gtttgcgcac agtcatggta tcgagtggct
      841 tgccttgctt ctcttcttgg cggatggtgc gcagcaattg gcggctttgc tcttcgctgt
      901 ctcctgtaaa cgcgccaaag cgagtttgta agtcttggtt gatgcggctt aacaaggcat
      961 cgcgcaaatc aaaataattg cggcctttgg gagcgatata gcccagatag gtaaaggttt
     1021 gggttaaaac atcacgatgg actttggcca agcgcagtgg caaggcgagg tgtggctgtt
     1081 gcggcaagcc cagttgttgc tcaacatagg ccagctcttg catcaactca cggctgacat
     1141 tgcggataaa acgcaatttc cataccacgg gctgtatcaa tacgggtttt tcaggccgct
     1201 cttgtgccaa ctccatggcc atttgtgctg cgccatgaaa caaagggttc acgtaatcgc
     1261 tttgccacaa tacctgacct tcagggtgta gcagcacagg tgtgccactg gcggcggctt
     1321 gtttggaata ggtcaacgct gcttcggtgt cacctggcac ttgggcaatc aaatggttgg
     1381 ccagccaaaa tttttgcccc catttaccca tgccattgac gatgctgtgg gtagcccagt
     1441 tggcggtgag tgggtagaaa cggctggcaa tttctttgtc gagcatccag tcggtgaaaa
     1501 attcggggtg gttgggcgtg atgaagctgg cgttgctgcc gttcatggca aaggccaata
     1561 aggctttgga ggtatcatca agatcgattt cacgtatgtc acacaaaccg cgcacaatgg
     1621 gcaggcgatt gagatacggg atgtcacgca agagcgggat gcctttgaga ttgaccaagc
     1681 ggttaaatgg aatcaatgtg cgtatcagac cagcatgtgg cttgggcgta acaaaagtgt
     1741 ttaaacgggg ggtgcccata aactgcctcc tgaggtatgt gaatcataca gcctgtaaaa
     1801 cttacaggcc gcatgattca tattgtttac tgttgcgtgg gtttgcggcg cgaatagccg
     1861 tacagtaaca gcaataagac agtgaagacg atggccaatg gccaagtact gcccaattgc
     1921 atatacggtg ttaaaccttg atacccttgt atggttacag tcagcacctg tttggtgtcg
     1981 cgcggcgcca aggcggtgat gtggccttgt gggtcgagta ccgcggtcaa acctgtgttg
     2041 gtagaacgca ccatgtagcg gcccagttct aaggcccgtg cttgcgattg ttgcaattgc
     2101 aagtccatcg catgcgaagt gccataccac gccatgttgc tgacattgga cagcagcgtc
     2161 gaggttttgg cagaggctat caaatcatcg ccaaaactgt cttcatagca aatgttgaac
     2221 gcgatttgct ggttggccaa ttgtaacgga gcttgattgt tgccaccttc gctaaagtca
     2281 gccaaaggca tgtgcatcat gcggtagagc caatcggtga aaatgggttt gaactcacca
     2341 aacggcacca aatggtcttt gctgtaagat ggcaaaggtt tatcggtttg ttcgggattg
     2401 aattcgctta aattgatgac cgagtttaaa tattggcggc ctgtaggcgt gtattgtggt
     2461 atgcctatgg ctaaggctgc attgttgcgt ttggctgtgc tggcgaattg ttccagcaag
     2521 ccatcgggca aatcttggcg catcacggga atggcggttt caggcaggat aacgatgtcg
     2581 gcgctggttt cggccaccat gtcgtaatag gtttggatgg tgtgctgaaa cgcctctgga
     2641 tcccatttca tgctttgttc aatattgcct tgcagcaagg ccacggtggc ggtgctgcca
     2701 tcggcttggg tccaacgctg ctgacccaac aacaagccta tccctatcac tacaatggcg
     2761 ctgatgaggt gtacggcttt ttgccacaca ggtttgcaca gtattaaggc agcgagcgcg
     2821 gcgctgacca ctgctgttgc ccaagtcacc aaagcgatgc cactgatggg tgcataggca
     2881 gccaagggtg attcggcgat ttggctgtaa ccaatcgcac cccagccaaa acctgttaag
     2941 gctcgttcac ggataaactc ggtgagcgtc caagccagtg gaaacaccaa taacaagcgc
     3001 cacactacag gcaagcgaat tttttcgcac agccaaatgg ccaaagtggg gtataaggcc
     3061 aaataagcgg gcaagagggc tgtgagggga atagaaatgt atgaaggcag acctgcgatg
     3121 tcgtgcaagg cggtattaat ccagtaacac tgcacaaaat aagcaatcaa tgcccacaca
     3181 tacaccatgc gcaaacggta ggcttggtat ttgacggcaa tcaccaacat ggccgcaacc
     3241 aaaatgggca tcagccaata caaacgatag ggcgcatagg caaacggggt cagcagtgcc
     3301 gagaccacca taacaaacca gccccaaacg gggctggtgc gagcaaataa gcgttggata
     3361 ttcaccatta atgggctgcc tttgtgccgc gcaaactgtt caccatgttc acaatccaaa
     3421 caatgatgcc accaacaatg gcacccacca acacatccaa gccatgtacc accaaaaatt
     3481 tcacaatgcc tgtgaattgg ctgtccaatt catgcgagat gtgcgccaaa aatggaatgt
     3541 tgtgcacaat gatggagccg cctaccaaga acatggcggc tgtgccagcg atggtgagga
     3601 agcgcatcaa ataaggggtg gagttaatta aaaaagcgcc aaatttttgc gccacggccg
     3661 atgatttttg tttcaaccaa aagccgacat cgtccatttt cacgatgccc gccaccaaac
     3721 catacacgcc tgcggtaaac acgatgctga tgatggccaa ggcgatggct tgctttaaga
     3781 tgggttcatg cgacatagag cccaaactga tggcgatgat ttcagccgat aaaataaagt
     3841 cagtgcggat ggcgcctttg attttgcctt tttcatcaaa agccacatcg gcaaccgtgt
     3901 cggcgctgtg ggtggcatcg tgtttgtggc gcattttgtg gatgctgtgc aagacttttt
     3961 ccacgccttc aaaagctaaa aatgccccac ccacaatcaa tacagggcct atcataaagg
     4021 gcagaaaata ggataaggcc aaaaacaagg gcaccaatat cactttgttc caaaaagagc
     4081 ctttggccac tgcccaaaca atcggcaatt ctcggttcga ggccgagcct gagacttgct
     4141 cggcattcaa agccaaatcg tcacccaaaa ccccagcggt ttttttggcg gcgactttgg
     4201 tcatcaccga tacatcgtct aacacggcgg caatgtcgtc caataacaac agtaaagatc
     4261 ctgccatata ttctaattct ttcttgtgtg atttttatga tgggtttggg gtgaccagca
     4321 atacgtgcaa acggcgggca tcggcacgag cgatggtaaa cagccatttg tcgatggcga
     4381 ctttttcacc gcgctgcggc aggcgcccaa actcatgtat gaccaagcca cctatggtgt
     4441 cggcctcttc atccgagtaa tcggttttga agtattcgtt gaagtcttcg atttcggtgg
//
LOCUS       DAOMVK010000148         4492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_5522_length_4492_cov_2.954699, whole genome shotgun sequence.
ACCESSION   DAOMVK010000148 DAOMVK010000000
VERSION     DAOMVK010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5522_length_4492_cov_2.954699"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..641
                     /locus_tag="PK856_06040"
     CDS             24..641
                     /locus_tag="PK856_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HRL01965.1"
                     /translation="MIDLLEAFFTEYGYVAVFTVLVACGFGIPIPEDVTLVVGGIMAG
                     LGHANVHVMVIVGIAGVLVGDGMMFAAGRIFGHRILRFRLVARVMTPKRYAQVQEKFE
                     KYGNWVLFIARFLPGLRTPIFISAGISGKVSYMRFLMMDGFAALISVPVWVYLGDFGA
                     ENSEWLWHKVHQFQMVLFVFLGVVLLYVGYRWWNKRQKAKIAESN"
     gene            complement(1012..2472)
                     /gene="guaB"
                     /locus_tag="PK856_06045"
     CDS             complement(1012..2472)
                     /gene="guaB"
                     /locus_tag="PK856_06045"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003783336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRL01966.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVSLKTPLTRNISLNIPLV
                     SAAMDTVTEAKLAIAIAQEGGIGIMHKNMSIERQAKAIAKVKRHESGIVKEPVTIAPN
                     MLVRDLIDLTRKHKVSGLPVVEDGKVVGIVTNRDLRFEKRLDQTVASIMTPKERLVTV
                     AEGSSIDAARDVMHDHKVERVLVINDSWELKGLITVKDILKTSTFPNANKDSNGSLRV
                     GAAVGVGAETDERVAALVAAGVDVIVVDTAHGHSQGVIDRVRWVKEHYPHVDVIGGNI
                     ATAQAARDLVAAGADGVKVGIGPGSICTTRIVAGVGVPQLTAIHNVSEALQGTSVPLI
                     ADGGIRFSGDVAKALAAGASAVMLGGMFAGTDEAPGEIELYQGRSYKSYRGMGSLGAM
                     SQGSSDRYFQDTVSSTDKYVPEGIEGRVPYKGPITNIIHQLVGGLRSSMGYLGCESIA
                     AMHERAEFVEITAAGMNESHVHDVQITKEAPNYHAR"
     gene            2633..3112
                     /locus_tag="PK856_06050"
     CDS             2633..3112
                     /locus_tag="PK856_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HRL01967.1"
                     /translation="MMKSTKTVLSLALISMVGMASANGVFTWKGKSGNQVYSDTPRNL
                     STQNVSTMNIRSHTVTKIETKPVGDTIQGGKIGVEGEVKEKSLAEQQSELNTKMAAEN
                     KKKEEENKKIDEQNKKVNEENCTRAKMNLNNVQTSNRVNEREKLAAGYQQDIDRYCK"
     gene            3126..>4036
                     /gene="uvrC"
                     /locus_tag="PK856_06055"
     CDS             3126..>4036
                     /gene="uvrC"
                     /locus_tag="PK856_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRL01968.1"
                     /translation="MSFDIATFLQSLPQLPGVYRMLDTAGQVLYVGKAVNLKRRVSSY
                     FQKTDLSPRIMLMVKQVASIEITVTRSEAEALILENNLIKALSPKYNILFRDDKSYPY
                     LIFTEHEYPQIAYHRGSLKGIKQFFGPFPNGYAVRQSMEVLQKVFQLRTCEDTVFAHR
                     ERACLLHQIKRCSAPCIAAINAEDYADTVQQATLFLQGRTDELMARLQAQMQQAAEQL
                     EFEQAGMLRDQIVALGALQSQQYVDVQAGNKVQDIDVLAAVAEQGLVCIHWSSIRGGR
                     LVGDKNYFPEQFDDAWQTLQQSAEMFVA"
     assembly_gap    4037..4046
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4047..>4492
                     /gene="uvrC"
                     /locus_tag="PK856_06060"
     CDS             <4047..>4492
                     /gene="uvrC"
                     /locus_tag="PK856_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958041.1"
                     /note="The UvrABC repair system catalyzes the recognition
                     and processing of DNA lesions. UvrC both incises the 5'
                     and 3' sides of the lesion. The N-terminal half is
                     responsible for the 3' incision and the C-terminal half is
                     responsible for the 5' incision; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit C"
                     /protein_id="HRL01969.1"
                     /translation="PQIIISDFEIPESLQAALNQEGHKLAYINQTTGQRKVWLKMARQ
                     NAVIAITQKQQQHSSQMLRLDALAEVLNMQDLNRLECFDISHTQGEATVASCVVYDEG
                     AMQPAQYRRFNIQTAKAGDDYAAMREVLTRRYGGLKEAAENGEAVL"
BASE COUNT         1189 a          954 c         1169 g         1170 t
ORIGIN      
        1 ataaagcctg aatcgccatg agtttgatag acttattaga agcatttttt actgagtacg
       61 gttacgttgc tgtgtttacc gtgttggtgg cctgtggctt tggtatcccc atacctgaag
      121 atgtaacttt ggtggtgggt ggtatcatgg cgggtttggg ccatgccaac gtgcatgtaa
      181 tggtgattgt gggcatagca ggggtgttgg ttggtgatgg catgatgttt gcagcgggcc
      241 gtatttttgg acaccgcatt ttgcgttttc gcttggtggc acgggtgatg acgcccaagc
      301 gttatgccca agtgcaagaa aagtttgaga aatacggcaa ttgggtgttg ttcattgccc
      361 gattcttacc cggtttgcgc acacccatat tcatttctgc gggcatcagt ggcaaggtgt
      421 cttacatgcg cttcttgatg atggacggtt ttgctgcttt gatttcggtg cctgtgtggg
      481 tgtatttggg tgatttcggt gctgaaaaca gcgagtggct gtggcacaaa gtgcatcaat
      541 tccagatggt cttgtttgtg ttcttgggtg tggtattgct gtatgtgggc taccgttggt
      601 ggaacaagcg ccaaaaagcc aagattgcag aatcaaacta gtattgctta gctgtaattg
      661 aaaagcggcc tgtaaatgtt tacaggccgc tttgtattca tgtttgcttg taaattgagt
      721 atcgtataac cgagattcaa tgggtatttg ggtaagagag atatttgtat tgaggcctta
      781 tgcacacgaa tgtagcgcga tggaatgtga taatcatctc gtgtttggta ctgtgttggg
      841 cgatgtatta atggacatca ccatactgtg atgctttctg ccattggaat gtaagtgttt
      901 agatttgcat ttacatacaa ggtgtcagtg aggtatggta aaagctgtta agcaaagaaa
      961 agcggcctgt aaactttaca ggccgcttaa ctattacaga tttaaaccaa cttaacgagc
     1021 atggtagttg ggtgcttctt tggtgatttg cacatcgtga acatgagatt cgttcatgcc
     1081 cgcagcggtg atttccacaa attcagcacg ttcatgcata gcagcaatgg attcgcaacc
     1141 caagtagccc atgctggaac gcaagccacc caccaattgg tgaatgatgt tggtaatcgg
     1201 gcctttataa ggcacacggc cttcaatgcc ttcaggcacg tatttgtcag tgctggaaac
     1261 agtgtcttgg aagtagcggt ctgatgaacc ttggctcatc gcgcccaaag agcccatgcc
     1321 acggtaagac ttgtatgaac ggccttggta caattcgatt tcgccagggg cttcatcggt
     1381 acctgcaaac ataccaccca acatcaccgc agaggcgcca gcagccaagg ctttggccac
     1441 gtcacctgag aagcggatgc cgccgtcggc aatcagaggc acacttgtgc cttgcaaagc
     1501 ttctgatacg ttgtggatgg cagtcagttg tggtacaccc acgcctgcta cgatgcgggt
     1561 agtacagatt gaaccaggac cgatgccgac tttcacgcca tccgcacccg cagccactaa
     1621 gtctcgggca gcttgcgcag tggcgatgtt gccaccaatc acgtccacgt gagggtaatg
     1681 ctctttcacc cagcgcacac ggtctatcac gccttggctg tggccatgcg ccgtgtctac
     1741 cacgatgaca tccacaccag cagcgaccaa agctgccaca cgttcgtctg tttctgcacc
     1801 cacgcccaca gcagcaccga cgcgtaaact gccattgctg tctttgttgg cattgggaaa
     1861 agtggaggtt ttcaaaatgt ctttaacggt aatcaggcct ttgagttccc agctgtcatt
     1921 aatgaccaag acgcgctcaa ctttgtggtc atgcatcaca tcacgggcgg catcaatgct
     1981 gctgccttca gcaaccgtca ccaggcgctc tttcggcgtc atgatggaag ccacggtttg
     2041 gtctaagcgt ttttcaaagc gcaaatcgcg gttggtcaca ataccaacca ctttgccatc
     2101 ttctactaca ggcaagcctg atactttgtg cttgcgagtc aaatcaatca aatcacgcac
     2161 cagcatattg ggggcaatgg tcacaggctc tttgacaatg ccgctttcat ggcgtttgac
     2221 ttttgcaatg gctttggctt gacgctcaat cgacatgttc ttgtgcatga taccaatgcc
     2281 accttcttgg gcaatggcta tggccaattt ggcttcggta accgtgtcca tggcggcaga
     2341 cacaagaggg atgttcagag agatgttgcg ggtgagtggg gttttgagag agacgtcgcg
     2401 tggcaaaacg gtagaatgag ccggtaccag taaaacgtcg tcgaaagtat aggctttttc
     2461 gacaatgcgc atttggaact tctttcttgg tcaacaatgc cgaattgtat caaatttgtt
     2521 ggtttacgtt aagcattttg ccaaagtaag acgtttaaat ggcatctgta gtgagtttgc
     2581 agttaaaatg gaaagcagtt gaacataatg ataaccatag ggaatatttg acatgatgaa
     2641 atccacaaaa acagtgctga gtctggcttt aatcagtatg gtgggcatgg cttcagccaa
     2701 cggggtgttt acttggaagg gtaaaagcgg caatcaagtg tattcagata caccacgcaa
     2761 tttgagtacg caaaacgtga gcaccatgaa catccgctca cacaccgtga ccaaaatcga
     2821 aaccaagcca gtgggtgata ccatccaagg cggtaaaatt ggtgtggaag gtgaagtaaa
     2881 agagaaaagt ttggctgagc aacagtcaga attgaatact aagatggctg ctgaaaacaa
     2941 gaaaaaagaa gaagagaaca agaaaattga tgagcaaaac aaaaaagtca atgaagagaa
     3001 ctgtacccga gccaagatga acttgaataa tgttcaaacc agtaaccgtg tgaatgagcg
     3061 tgaaaagttg gcggcaggtt atcaacaaga cattgatcgt tactgtaaat aatcttggag
     3121 gctgaatgtc gtttgatatt gcgacgtttt tacaaagctt acctcaattg cccggtgtgt
     3181 atcgcatgtt ggatacggcc gggcaagtgc tttatgtggg taaggcagtg aatttaaaac
     3241 gtcgggtcag cagttacttt caaaaaacag atttgtcacc acgcatcatg ttaatggtga
     3301 aacaagttgc cagcattgaa atcaccgtca cgcgttctga ggcagaggct ttaattcttg
     3361 aaaacaattt aattaaggct ttaagtccga aatacaacat cttgtttcga gatgacaagt
     3421 cgtatcccta tctgattttt accgaacacg aatacccaca aatagcttac caccgaggca
     3481 gtttgaaggg gattaaacag ttttttgggc catttcccaa tggctatgca gtgcgccaaa
     3541 gcatggaagt gttacaaaaa gtgtttcaac tgcgtacttg cgaagacacg gtatttgccc
     3601 atcgagaacg cgcgtgtttg ctgcatcaga ttaagcgctg tagtgcacct tgtattgcgg
     3661 ccatcaatgc ggaagactat gccgatactg tgcagcaagc gactttgttt ttgcaaggac
     3721 gcaccgatga attgatggcg cgcttgcagg cacaaatgca gcaagctgcc gaacagttgg
     3781 agtttgaaca agcaggcatg ttacgtgacc aaattgtggc attgggcgct ttacaatcgc
     3841 agcaatacgt ggacgtacag gccggtaata aggtgcaaga cattgatgtt ttggctgccg
     3901 tagccgagca aggcttggtg tgcatacatt ggagcagcat ccgtggtggg cgtttggttg
     3961 gcgacaaaaa ctatttccct gagcaatttg acgatgcttg gcaaaccttg cagcaatcag
     4021 cagagatgtt tgtggcnnnn nnnnnnaccg caaatcatca tcagtgattt tgaaatccca
     4081 gagagtttac aggccgcttt aaatcaagaa gggcacaaac tggcttacat caatcaaacc
     4141 acggggcagc gcaaagtgtg gttgaaaatg gccaggcaaa atgcggtgat tgccatcacc
     4201 caaaagcagc agcaacacag ctcacaaatg ctgcgtttgg atgctttggc cgaagtgctg
     4261 aacatgcaag acttgaatcg cttggagtgt tttgacatca gtcatactca aggtgaagcc
     4321 acagtggcat cgtgtgtggt atatgacgag ggagcaatgc aacctgcaca ataccgacga
     4381 ttcaatattc aaacagcgaa agctggcgat gattatgctg ccatgagaga ggtgttgacc
     4441 cgtcgctatg gcggcctgaa agaagcggct gaaaatggtg aagctgtttt at
//
LOCUS       DAOMVK010000149         4473 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_5559_length_4473_cov_2.797646, whole genome shotgun sequence.
ACCESSION   DAOMVK010000149 DAOMVK010000000
VERSION     DAOMVK010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5559_length_4473_cov_2.797646"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="PK856_06065"
     CDS             complement(<1..512)
                     /locus_tag="PK856_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRL01970.1"
                     /translation="MEELRDWLPMWAHDSLDLMLVVLQIAGIVFVAWFARRILRKLLN
                     RMVDNQHLPVDIAVPLKTIGGWLIMVTAALWILEILGISGRVLWTAITGFAAVAAVAF
                     FAAWSVLSNFFCAFLIFTTQPFRVGDELEILDASDKVGMHGRVLAIHLLYIVLEEIGR
                     EDGRVTLIQVP"
     gene            694..1218
                     /gene="hemG"
                     /locus_tag="PK856_06070"
     CDS             694..1218
                     /gene="hemG"
                     /locus_tag="PK856_06070"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF008316.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HRL01971.1"
                     /translation="MHILILYSSRFGQTLKIAQALQHQWQAEGAQVTLTNLEHTAGLS
                     LSDFDKVVIGASIRYGHFAKCLNTFTAQHAQALNHIPSAFFSVSILANKAHKSTAETH
                     TYTRKFFEQSEWKPKQIGIFAGELAYHKYHLVDRYLMKLVMKINKGETNTRAHIEYTD
                     WQKVHQFGVSVLSM"
     gene            complement(1278..2075)
                     /locus_tag="PK856_06075"
     CDS             complement(1278..2075)
                     /locus_tag="PK856_06075"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRL01972.1"
                     /translation="MTKIHLELEQLLHLEHEPAHPIHDLSSFVQGVQVLMPLINGLPN
                     ITFFIKDLQARYVLANVNLAVRSGLNCVDDLLGKTAAEVFSSEIGMAFIQQDQLVMSG
                     QPLSQHLELHLYQSGLLGWCVATKIPLKNGQGEVLGMAGMAVDLQEDRLNRPNINSKL
                     SKVEQYISEKFDTPIQITELANIAGLSVSQLNRQFQNIFHLTPSQLIQKKRLDCAIEL
                     LAQDISVTEVSVRCGYADHSAFGRKFKELTSVSPRQFKQQLLKLKQE"
     gene            complement(2171..3481)
                     /gene="abaF"
                     /locus_tag="PK856_06080"
     CDS             complement(2171..3481)
                     /gene="abaF"
                     /locus_tag="PK856_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosfomycin efflux MFS transporter AbaF"
                     /protein_id="HRL01973.1"
                     /translation="MEKTEDHSQLKKVVAASMIGSVAEWYEFFLYGTASALVFGQLFF
                     QQTGNAIDGILAAFALYAVGFLARPLGGLVFGHYGDKIGRKKLLQLSLILVGITTFLM
                     GCIPTFQSIGYWAPVLLVGLRLIQGFAFGGEWGGAVILVSEHSPADRRGYWASWPQTG
                     VPLGNLVATIILLILSKYLTPEAFLDWGWRVAFWFSAVVVLIGLWIRKSVDDAPVFKE
                     AQEKQAQLEKQQLGIVEVVKNHKRAIVAGIGARFAENILYYMVVTFSISYLKLVVHKD
                     TTEILLLMFGAHAVHFFFIPLMGHLSDKVGRKPIYIIGAVLTAFWGFVGFPLMDTGND
                     WLIMLAIIFGLFIQSMTYSPYSALMTELFPTHIRYTALSLCYQVAPILAGSWAPLISL
                     SLLKEYNSSIPISIYLVGASLISIFCIMLVKETKGKELTFEKAE"
     gene            3914..>4473
                     /locus_tag="PK856_06085"
     CDS             3914..>4473
                     /locus_tag="PK856_06085"
                     /inference="COORDINATES: protein motif:HMM:NF018691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRL01974.1"
                     /translation="MTAITLAGALANRGLRVGLADADPQKSSLQWLKYRPDHAAHIQA
                     VDWRQEKAIGEAPKDLDFLIVDAPGALSGEHAELLVSESHAIVTPLQPSVFDIDSTKR
                     FLKHLQDIKRIRKGKVKILLLANRLKPNTTSVESIKAFFQQIEQNPAAAILERSCYDQ
                     LAQEGLSVFDKTQKQYTQVQDQWRPL"
BASE COUNT         1325 a         1149 c          922 g         1077 t
ORIGIN      
        1 gggacttgaa tcaaggtgac acgtccatct tctcgcccga tttcttcaag cacaatataa
       61 agcaagtgta ttgccaaaac acggccatgc atgcctacct tatcggatgc atcaaggatt
      121 tccaactcat cgcccactcg aaacggctgg gtggtgaaaa tcaaaaacgc acaaaaaaag
      181 ttggataaca cactccaagc ggcaaagaat gccacggcag ccaccgctgc aaaacccgtg
      241 attgcggtcc acaaaacacg cccagaaatc cccaagattt ccagtatcca caaagcagcc
      301 gtcaccataa tgagccagcc gcctatggtt ttcagtggca cggcaatgtc cacaggcaag
      361 tgttgattat caaccatgcg gttcaacagt ttgcgcaata ttcggcgcgc aaaccaagcc
      421 acaaagacaa tgccagcaat ttgcagcact accaacatca aatccaaaga gtcatgtgcc
      481 cacatgggca accagtcgcg caattcttcc atagtcactc cttaagcgcg catcatatca
      541 taagctttca gctgagttta caggccgcct cagaatcgca ataagcggcc tgtaagccat
      601 accgtcatga atcaatcaag ctgagcagca aatgctgacg atagcgttta taatgctctg
      661 cattcttgca gttaacgctt tttgagttct gatatgcaca ttttaatttt gtactccagc
      721 cgcttcggcc aaacccttaa aatcgcccaa gccttacaac atcagtggca agctgaaggc
      781 gcgcaagtga cgctcaccaa tctggagcac acggcaggtt taagtctgtc agactttgac
      841 aaagtagtca ttggcgcttc catacgctat ggacattttg ccaagtgcct gaacacgttt
      901 actgcgcaac atgcccaagc cttaaatcac atccccagcg ccttttttag cgtcagcata
      961 ttggccaaca aagcacacaa atccaccgca gaaacccaca cttacacacg caaatttttt
     1021 gaacaaagcg aatggaaacc caaacagata ggcatttttg ctggcgagtt ggcctaccac
     1081 aaataccatt tggtcgaccg ctacctgatg aaattggtga tgaaaatcaa caaaggcgaa
     1141 accaataccc gtgcgcatat tgaatacacc gattggcaaa aagtccatca atttggtgtc
     1201 agtgtgctga gcatgtaaaa cacgccaata tgcggcctgt aaggcatgtg gattgcatca
     1261 ccaacatgca aaccgcttca ctcctgcttg agtttcaaca attgctgttt aaactgcctg
     1321 ggcgagactg aggtcaattc cttgaacttt cggccaaatg cgctgtgatc ggcatagcca
     1381 cagcgcaccg aaacttcggt caccgagatg tcttgcgcca gcaattctat ggcgcaatcc
     1441 aagcgttttt tctgtatcaa ttgtgatggt gtcaggtgaa aaatgttctg aaattgtcgg
     1501 ttaagctgcg acactgatag tcctgcaatg ttagccagtt ccgtgatttg tatcggcgta
     1561 tcaaatttct cactgatgta ttgttcaacc ttgctgagct tggaattgat attgggtcgg
     1621 ttcagcctgt cctcttgtaa atccacggcc atgcccgcca tgcccaagac ctcaccttgg
     1681 ccgttcttca acggaatctt ggtagccaca caccaaccca acaaacccga ttgatacaaa
     1741 tgaagctcca aatgctgact taagggctgc ccactcatca ccaattggtc ttgctgtata
     1801 aacgccatgc caatttcaga agaaaagact tcagccgcag ttttacccaa taaatcatcg
     1861 acacagttta aaccagagcg cacggccaaa ttcacattcg ccaacacata tctggcctgt
     1921 aaatctttaa tgaaaaaagt gatgttgggc aaaccgttaa tcaaaggcat cagcacttgc
     1981 acaccttgca caaatgagga caagtcatgt atgggatgag caggctcatg ttccaaatgc
     2041 aataactgtt ctagctccaa gtgtattttg gtcatggtca tcaggctgtg tatttaagga
     2101 attcaggtat tgctcaaggg cttttccaat gaaaaaaccc acccctagag gtgggtctac
     2161 tttaaaacag ttattctgct ttttcaaatg tcagttcttt acctttggtt tccttaacca
     2221 acatgataca gaagatggaa atcaaactcg cgcccaccaa gtaaatcgag ataggaatgg
     2281 agctgttgta ctctttgagc agggacaagg aaatcaatgg tgcccatgaa cctgccaaaa
     2341 tcggtgccac ttggtagcac agcgacaagg ctgtgtaacg gatgtgggtt gggaacagtt
     2401 ccgtcatcaa ggctgaatat ggcgagtaag tcattgattg aatgaacaga ccaaaaataa
     2461 tcgccaacat gattaaccag tcattgcctg tgtccatcaa tgggaaaccg acgaagcccc
     2521 agaatgccgt caataccgca cctatgatgt aaatcggctt gcggcccact ttgtcgctca
     2581 agtgccccat taaaggaatg aagaagaaat gcacagcatg cgcgccaaac atcaacaaca
     2641 ggatttcggt ggtgtctttg tgaaccacca atttcaaata actgatggag aaagtcacca
     2701 ccatgtaata caagatgttt tcggcaaaac gggcaccaat accagccact attgcacgct
     2761 tgtgattttt taccacttca acaatgccca attgttgctt ttccaattgc gcttgttttt
     2821 cttgagcctc tttgaatacg ggtgcatcgt ccacgctctt acgtatccac aaacctatca
     2881 acaccaccac tgcggagaac cagaaagcca cgcgccagcc ccaatccaag aaggcctcag
     2941 gggtcaagta tttagaaagg atgagcaaga tgatggttgc caccaagtta cccaatggca
     3001 cgcctgtttg cggccagctt gcccagtagc cacgacggtc tgcagggctg tgctcagaca
     3061 ccaaaatcac cgctccgccc cactcgccac caaaagcaaa gccttgtatc aaacgcaaac
     3121 ccaccaataa tactggtgcc caatagccta tgctttgaaa tgtgggaata caccccatta
     3181 aaaatgtggt gatgcccacc aaaatcaaac tcagttgcag cagtttttta cggccaattt
     3241 tgtcgccata atgtccgaat accaaaccac ccaaaggccg agccaaaaag cctactgcat
     3301 acaatgcaaa agccgccaaa atgccatcta tggcattgcc tgtttgttgg aaaaacaatt
     3361 gcccaaacac caaggcagac gctgttccat acaagaaaaa ttcataccat tctgcgaccg
     3421 aacctatcat cgaggccgca accacctttt tcagctggct atgatcttcc gttttctcca
     3481 ttccactctc cgtcaatcat tattatgaat aagtgtttgc agctcagggc ttgtctgatt
     3541 gaaacggcgc caagccattt catttttttg ctcttcaccc ataactgcaa acgcagagcg
     3601 tagagtgctt gatttactgg ggagaatctt gatgtattta acccgattga tgcgcgattt
     3661 ggcagagttt gatctgtgtc agtttcatga atgtcagtga aattgcttgt tttaatgccc
     3721 ataagcggcc tgtaaactag aaataatgtt acagaccgct tctaaaccca tccaattgat
     3781 tgaataagca acaacacacg cagtcatcat attgtcacca aacaatgcta agctgtgcat
     3841 gggcaataga ctgtgaatga aaacaatgaa aaccatctta gtagcaaatt taaaaggcgg
     3901 atgtggaaaa accatgacag ccatcacatt ggcaggggct cttgccaacc gcggtttgcg
     3961 cgtgggctta gccgatgcag atccacaaaa atcctcgttg caatggttga aataccgacc
     4021 agaccatgcc gctcacattc aggctgtgga ctggcgccaa gaaaaagcca taggtgaagc
     4081 ccctaaggac ttggattttc tgattgtgga tgcgccaggt gctttatctg gcgaacatgc
     4141 cgagttgctg gtgagcgaaa gccacgccat tgttacccca ttacaacctt ctgtttttga
     4201 catagacagc accaaacgct tcttaaaaca cttacaagac atcaagcgca tacgcaaagg
     4261 caaggtaaaa atcctgttat tggccaatcg cctaaagccc aacaccacca gtgtggaaag
     4321 cattaaagcc tttttccagc aaattgaaca aaatccagcc gcagccattt tagaacgctc
     4381 ttgctacgac cagttggcac aagagggctt aagcgtgttt gataaaactc agaaacaata
     4441 tacccaagta caagaccaat ggcgcccttt att
//
LOCUS       DAOMVK010000150         4465 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_5570_length_4465_cov_2.595011, whole genome shotgun sequence.
ACCESSION   DAOMVK010000150 DAOMVK010000000
VERSION     DAOMVK010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5570_length_4465_cov_2.595011"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1237
                     /locus_tag="PK856_06090"
     CDS             <1..1237
                     /locus_tag="PK856_06090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D C-terminal
                     domain-containing protein"
                     /protein_id="HRL01975.1"
                     /translation="DMLKILHTSDWHLGRSLYGQNRHAEFAAFLDWLMQTLQQERVDV
                     LMIAGDVFDTITPNHAAQELYYRFLAQVSAYCLCRHIIIIGGNHDSASFLNAPKALLF
                     ALNVHVIGAATNPIDKEVLVLKNAQGEPAAIVCAVPYLRDRDVRVSVDGESSQDKSLA
                     LTQGIKDHYRQALAIAEHKQQELGSNAPIIAMGHLFSQGGMTLDADGMRELYVGSLAH
                     IGADCFDGFDYVALGHLHVPQAVGGQAHIRYCGSPIPMGFGEAKQQKQVLHVAFTGRL
                     PEINAINIPNFQVLQRIQGDLPHIQAELMQLMHAGSNAYVEVVYQGNELVADLSEKLD
                     AQVQGSDLRLLRVYNRQFVNNVLASASADATLPDLNPTEVFEACMAQHDLDDAQKAQL
                     RDSYQHVLHQVEQPEEDEA"
     gene            1234..>4465
                     /locus_tag="PK856_06095"
     CDS             1234..>4465
                     /locus_tag="PK856_06095"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRL01976.1"
                     /translation="MKILSLRFENLNSLYGVWSVDFQHRDYTNNGLFAITGPTGAGKS
                     TLLDAICLGLFGQTPRLKTISKSENEIMSRHSGHCFAEVVFAVGDKTYRSTWSQSRAR
                     NKPDGRLQEAKHELADASTGKIIEEKKSRTVAAIEAISNLNFQRFTRSVLLAQGSSAA
                     FLQASVDERSDMLQQITGTEIYEHISKQVHQNKRDAESALHTLQAVLEGITLLDDGEI
                     HTLQQSHAEHATQCETLSSQLGQVREQQQRQLRWQHLQTQHATLQQQWQQHQTQNEQF
                     AADAKRLEQGLRAQKLGADYRELTLLRQRQHQLSQQQQHLQAALPQLAEQAIATQQQH
                     DVVHTQLKHLLEQHALRQDLWQRVGVLDARISTAQERKQEWQQQHSHLTQQAQQLAGK
                     RNKLQSQKDQHQEQLQRVEQQLAVQNMQIAAEQLPKWQEQLLQMERLQLLLTEQSQSI
                     ESIAQQIQLNTSAQQDLNRFFGNTEDRQQQLQQQMLQTRTEMEHLLAGKTLNELELER
                     EHLKKELTLMQKIVSLEHERQLLQQGAACPLCGSTEHPYVSEHVGNGEINATEARLQA
                     LDNLFKSHAECQHNLQQWYNNQSLLQQQYEMKQAEQLRLLTEAKTLAASHEKACAQSQ
                     NTVKQISTLTQGWAAFGYQEGDDAPLLLDQLQRRVKHWQRYQQLRQELQDQLHDLEKD
                     LLVASQQNQDTLLQLQQIDLESTAQQQLLQQLHNERHEVFGDANTEQDQQQWRQRVDT
                     AQQQWDEMREQWQHCRDEHQQHSQRLQDTTELWQQGQTALAATEEQWQQALQLHQFVD
                     EASWQSQCLPEVECEALSQQAQRLREEGIQLTHALEHNAQEQARLDTVGLDEAQQLAL
                     QTELNTLQAAYQHVTQAMGAITQQLHQQQLAQERMAEQAHKIHLQQQECARWRDLHEL
                     IGSSDGKKYRLFAQSLTFNQLVQQANRQLQNMSDRYLLKQTDDAPLQLDVIDQYQGGT
                     VRSAKNLSGGESFIVSLALALGLARMASATTRIDSLFLDEGFGTLDEDALDVALNTLA
                     SLQQEGKLIGVISHVAALKERISTQIKVEPKHNGHSVLHGSGIAHAS"
BASE COUNT         1271 a         1220 c         1103 g          871 t
ORIGIN      
        1 agacatgcta aagatattac acacatccga ttggcactta ggccgcagcc tgtatgggca
       61 aaaccgccac gcagaatttg ctgcgttttt agattggctc atgcaaaccc tgcaacaaga
      121 gcgggtggat gtgttgatga ttgccggcga tgtgtttgac accatcaccc ccaaccacgc
      181 tgcacaagag ctgtattacc gctttttggc gcaagtgtcg gcatattgcc tgtgtcgcca
      241 catcatcatc atcggtggca accacgattc cgccagcttt ttaaatgccc ccaaagcctt
      301 gttatttgcc ttgaatgtgc acgtcatcgg cgcggcaacc aaccccatag acaaagaagt
      361 cttggtcttg aaaaacgccc aaggcgagcc tgctgccatt gtgtgcgccg tgccttactt
      421 gcgcgaccgc gatgtgcgcg tgagtgtgga tggtgaaagc agccaagaca aaagcctggc
      481 cttaacccaa ggcatcaaag accattaccg ccaagcattg gccattgccg aacacaaaca
      541 gcaagaattg ggcagcaatg cgcccatcat cgccatgggc catttgttca gccaaggcgg
      601 catgacgctg gacgcggatg gcatgcgcga actgtatgtg ggcagtttgg cccacatcgg
      661 tgccgactgt ttcgatggct ttgattacgt ggccttgggg catttgcatg tgccgcaagc
      721 cgtgggtggc caagcgcaca ttcgctattg cggctcgccc attcccatgg gttttggcga
      781 ggccaagcag caaaaacaag tgttgcacgt ggcgtttaca ggtcgtttgc ctgagattaa
      841 cgccatcaac atccccaatt ttcaagtgtt gcaacgcatc caaggcgact tgccacacat
      901 acaggccgaa ttgatgcagc tcatgcacgc aggcagcaat gcgtatgtgg aagtggttta
      961 ccaaggcaat gaactggtgg ccgacttgag cgaaaaactc gatgcccaag tgcaaggcag
     1021 cgacttgcgc ttattgcgcg tgtacaaccg ccaatttgtg aacaatgtgt tggccagtgc
     1081 cagcgctgac gccaccctgc ccgatttgaa tcccaccgaa gtgtttgagg cgtgcatggc
     1141 acaacacgat ttggatgacg cgcaaaaagc ccaattgcgc gacagctacc aacacgtctt
     1201 gcaccaagtc gagcagccag aggaggacga ggcatgaaaa tcctcagctt gcgctttgaa
     1261 aacctgaatt cgctctacgg cgtgtggtcg gtagactttc aacaccgcga ttacaccaac
     1321 aacggcttgt ttgcgattac cggcccaact ggcgccggta aatccacctt gctagacgcc
     1381 atttgcttgg gtttgtttgg gcaaaccccg cgcttaaaaa ccatcagcaa aagcgaaaac
     1441 gaaatcatgt cgcgccacag cggccattgc tttgccgaag tggtgtttgc cgtgggcgac
     1501 aaaacctacc gcagcacttg gagccaaagc cgcgcccgca acaaacccga tggccgcttg
     1561 caagaagcca agcacgaatt ggccgatgcc agcacaggca aaatcattga agaaaagaaa
     1621 agccgtaccg ttgccgccat cgaagccatc agcaatttga atttccaacg tttcacccgc
     1681 tcggtcttgc tggcgcaagg cagttctgcc gcttttttac aggccagcgt ggacgagcgc
     1741 tccgacatgt tgcagcaaat cacgggcacg gaaatctacg agcatatttc caaacaggtt
     1801 caccagaata aacgcgacgc tgaatccgcc ttgcatacct tacaagcggt attagaaggc
     1861 atcaccttgc tggatgacgg tgaaattcac accttgcagc aaagccatgc tgagcatgca
     1921 acacaatgtg aaaccctctc gtcacagctc ggccaagtgc gtgagcaaca acagcgccaa
     1981 ctgcgctggc agcacttgca gacacaacat gccacgctgc aacagcaatg gcagcagcat
     2041 caaactcaga acgagcagtt tgcagcagat gccaaacgtc tggaacaagg cttacgggcc
     2101 caaaaactgg gggcggatta tcgcgaactg acgctgctgc gccaacgcca acaccaatta
     2161 tcacaacagc agcaacattt acaggccgcc ttacctcaat tggcagagca agccatcgcc
     2221 acccagcaac aacatgatgt ggtgcacact cagcttaaac atctgctgga acaacatgcg
     2281 ttgcgccaag atctgtggca gcgtgttggt gttttggatg cccgcatcag caccgcccaa
     2341 gaacgcaagc aagaatggca acaacaacac agccacctca cccaacaagc gcaacaactg
     2401 gctggcaagc gcaacaaact gcaatcgcaa aaagaccagc atcaagaaca attgcaacgg
     2461 gtagaacagc aattggcggt gcaaaacatg caaattgcag cagagcagct gcccaaatgg
     2521 caagagcaac tgctgcaaat ggagcgtttg caactcttat tgacggagca atcccaatcc
     2581 atcgaaagca ttgcccaaca aattcaactc aacaccagcg cccaacaaga cttaaaccgt
     2641 ttttttggca acactgaaga caggcaacaa caattgcagc aacaaatgct gcaaacccgt
     2701 accgaaatgg aacacttatt ggcgggaaag acattgaatg aattggaact ggagcgtgag
     2761 cacttaaaaa aagagctcac actcatgcaa aaaatcgtgt ctttggagca tgaacgccaa
     2821 ctgttacagc aaggtgctgc ttgccctttg tgcggctcca ccgaacaccc ttatgtcagc
     2881 gagcacgtgg gcaatgggga aatcaatgcc accgaagcgc gtctgcaggc attggacaac
     2941 ctgtttaaat cccatgccga gtgccaacac aatttgcaac aatggtataa caaccaaagc
     3001 ctgttacaac aacagtatga aatgaaacaa gcagaacaat tgcgcttgct gaccgaagcg
     3061 aaaacccttg ctgccagcca tgaaaaagcc tgtgcgcaaa gccaaaacac cgtcaagcaa
     3121 atctcaacct tgactcaggg ctgggctgcg tttggttacc aagaaggcga tgatgcccca
     3181 ttgttgttgg atcaattgca acggcgtgtg aagcattggc agcgctacca acaactccgc
     3241 caagagttac aagaccaact gcacgacttg gaaaaagact tgctggtggc ctctcagcaa
     3301 aatcaagaca ccttgctaca actgcaacaa atagacctag aatcgacggc tcaacaacag
     3361 cttctacagc aattgcataa tgagcgtcat gaggtttttg gtgatgctaa caccgagcaa
     3421 gatcagcagc agtggcgcca acgagtagac acagcgcaac agcaatggga tgaaatgagg
     3481 gagcagtggc aacactgccg agatgagcat caacaacaca gccaacgctt gcaagacacc
     3541 actgagcttt ggcaacaagg ccaaactgcc ttggctgcca ccgaagaaca gtggcagcaa
     3601 gccttgcaac tgcatcaatt tgttgatgaa gcctcgtggc aaagccaatg cttgccagag
     3661 gtcgaatgtg aggccttgag ccagcaggcg cagcgtttgc gcgaagaagg catacaactg
     3721 acgcatgcgt tggagcacaa tgcccaagaa caagcccgtt tggacactgt gggtttggat
     3781 gaagcacaac aactggctct tcaaaccgaa ttaaacactt tacaggccgc ttaccaacac
     3841 gtcacgcaag caatgggcgc cataacccaa cagttacacc aacaacagtt ggcgcaagag
     3901 cgcatggctg agcaagcaca caaaatacac ttgcaacagc aagaatgcgc tcgttggcgc
     3961 gatttacatg aactcatagg cagcagcgat ggcaagaaat accgcttgtt tgcccaaagc
     4021 ctgacgttta accaattggt gcagcaagcc aatcgccaat tgcaaaacat gagcgaccgc
     4081 tatttgctca agcaaaccga cgatgcgcct ttgcaattgg atgtgataga ccaataccaa
     4141 ggcggcacgg tgcgctcggc gaaaaacctc tcaggtggtg aaagctttat cgtgtctttg
     4201 gctttggcac tgggcttggc gcgcatggcc agcgccacca cgcgcataga ctcgctgttt
     4261 ttggatgaag gctttggcac cttggacgaa gacgcgctcg atgtggcctt gaataccttg
     4321 gccagtttgc agcaagaagg caaattaatc ggcgtgattt cgcatgtggc cgcattgaaa
     4381 gaacgcatca gcacccaaat caaagtcgaa cccaaacaca atggtcacag tgttttacac
     4441 ggcagtggca tagcccatgc ttcac
//
LOCUS       DAOMVK010000151         4449 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_5604_length_4449_cov_3.030496, whole genome shotgun sequence.
ACCESSION   DAOMVK010000151 DAOMVK010000000
VERSION     DAOMVK010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5604_length_4449_cov_3.030496"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            641..1600
                     /locus_tag="PK856_06100"
     CDS             641..1600
                     /locus_tag="PK856_06100"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HRL01977.1"
                     /translation="MKLKSSLYIGALIAASLNLSACFELPRSQAIESTRANIEDILDR
                     LPNNPSVDCAQDVLMEGIPPQINQNTLNSNTQNLCFEEFALKHSGISKGPLWVAEYLN
                     PEKVANKVKRQGEFHAESRLPPSKRAELSDYKGSGYDRGHLAPSGDMSTKSAQQDSFS
                     LANMVPQNPKNNQQAWRNIEEAVRQVVQNSNEPAYLITGVAFLNAKVPTIGANKVLVP
                     SHLYKAVYQPVNGVISAYWIANTASAQAQIISVCDLESKTGINVFPVLSKGERRQRYD
                     LPLTAAEVNKLGSIASLGRDSNHQCSNKLTATEAAKLAASFAQ"
     gene            1621..>1807
                     /locus_tag="PK856_06105"
     CDS             1621..>1807
                     /locus_tag="PK856_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01978.1"
                     /translation="MTFLAFADDCSSYTLDNLTFENNADRLSVYGDMQIQRDQQGLQQ
                     LLALQSLINQAVKALQAD"
     assembly_gap    1808..1817
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2037..2492
                     /locus_tag="PK856_06110"
     CDS             2037..2492
                     /locus_tag="PK856_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL01979.1"
                     /translation="MKPLIKPLWLASCLLALSTSVWAAPRLISAEPNTEHYSGADVDQ
                     TIILKNQDMNAKVFSYSGGDPAINGAYLNLAVYDEDKRGWNVFVLKDVLDYKVLPSAQ
                     QGYAKIALITQDVDQRSGNIRSYKSTLFINLRNANQRGGKIEVDEIKQK"
     gene            complement(2590..3339)
                     /locus_tag="PK856_06115"
     CDS             complement(2590..3339)
                     /locus_tag="PK856_06115"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HRL01980.1"
                     /translation="MKQAWQSVLLVLCFSMGLSACNASEVAVQTVHANNGVAIDVIKV
                     EKLADLRLYLNNSGGYAYQSFAAVRGELPACERMLFGMNAGMFHANYQPVGLYVAQGK
                     QLTPLNTDTGFGNFFMQPNGVLAWNAEQAWIGKTADYAGQNLQAAYATQSGPMLVINH
                     QINPQFLPDATSKKIRNGVGIKGKTLYFAISQKPVSFYQFAQVFKDTLKTEQALYLDG
                     SISSLYLPQTGREDMRHFLGPMVTLIQPCSS"
     gene            complement(3400..3894)
                     /locus_tag="PK856_06120"
     CDS             complement(3400..3894)
                     /locus_tag="PK856_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HRL01981.1"
                     /translation="MATDYQRALHTLRHCIASDTEQQRFAVRTLSEAEWVEIAEATDM
                     RLPAAYYEFIRQIGVGEFFLRHAHQGMFCVYGADEMRQNQRFLAKHLLDEGINMDDAY
                     LMVGVHQSMGDWMGFNLARADERCFDVYDHAAPVDEYAEIANWRTFEEWVMAVVASNG
                     VASL"
     gene            complement(3993..>4449)
                     /locus_tag="PK856_06125"
     CDS             complement(3993..>4449)
                     /locus_tag="PK856_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HRL01982.1"
                     /translation="WEILAGGNEDSVLYHTLHEFPSMPETVEAELAKKWQAVREVREA
                     VNKEIETKRAAEVVGSSWQAAVSITAPEPTYGYLRALGEELRFVLMVSKVSVSEGEAL
                     SIVVNKAEGEKCERCWHYLPDVGSNSAHPCLCARCIDNVEGKGEERLLA"
BASE COUNT         1268 a         1217 c          885 g         1069 t
ORIGIN      
        1 tcagcagcga agaaaccgaa tattacgcac ttccccgcat accgtcaaca cctaccgcac
       61 aatttatttc aacaattcca taaaacaact gttttacaag gagttttaat tgcaattaat
      121 ttcaaccacc ctgtaaaacc acttacaagc agcctgtaat aacaatctca atcaaccaaa
      181 actcagtgaa gaaaacctca aaaatctaca aattggctga aattcatcac catccccaca
      241 aaaccctacc taccaaacca gtacttccat ccaattacta caaatcaaac ctactcaagc
      301 aacccaaatt catgcaaatc acccaatcca ttaaaaacag aacacataac aattaagcgg
      361 cctgtaaacg cttctagaat acttacaggc cgctttgctt taagaaactc aggcattcaa
      421 tcttaatatt tcgattcatc acaataacaa tatatatatg gtaatagaca atcattccca
      481 cacgaactca agattacctc cttaagccct ccccattcgt ataacccagc tgtcttattt
      541 cacaaactct cgttaaggct ataaaatcag tttgtcataa tatgcattta ttgatgactt
      601 cccatctttt cccattaagc cttagacaca agcattacgc atgaaattaa aatcttcctt
      661 atatataggt gctttgattg cagccagcct caatctcagc gcctgctttg aacttcctcg
      721 atcccaagcc atagaaagca cccgtgccaa tatcgaagac attttggacc gtttgcccaa
      781 taacccctct gtcgactgtg cacaagacgt cttgatggaa ggcataccgc ctcaaatcaa
      841 ccaaaacact ttgaacagca atacccaaaa tttgtgcttt gaagaatttg cattaaaaca
      901 ctcaggcatt tccaaaggac cattgtgggt agcggaatat ttaaaccctg aaaaagtggc
      961 gaataaagtc aaacgtcaag gtgagtttca tgcagagagc agactgccgc catccaaacg
     1021 cgccgagctg tccgactata aaggcagcgg ttatgaccgt ggccatttgg ctcccagtgg
     1081 tgacatgagc accaagtccg cccaacaaga cagtttttca ttggccaaca tggtgccgca
     1141 aaatcctaaa aacaaccaac aggcttggcg caatattgaa gaagcggtac ggcaagtggt
     1201 gcaaaacagc aacgaacctg cttacttgat tacgggtgtg gcttttttaa atgccaaagt
     1261 tcccaccatt ggtgcaaaca aagtattggt acccagccat ttgtacaaag cggtatacca
     1321 acccgttaat ggcgtcatca gtgcgtattg gattgccaac accgcttcag cccaagcaca
     1381 aatcatcagc gtgtgcgatt tggaaagcaa aacaggaatc aatgtgtttc ccgtactcag
     1441 caaaggcgag cgcagacaac ggtatgattt accactgacc gcagccgagg taaacaaact
     1501 gggtagcatc gccagccttg gtcgcgacag caatcaccaa tgcagcaaca agctgactgc
     1561 aacagaagcc gccaagctgg cagcctcatt tgctcaataa gcagatatta aggagtaaaa
     1621 atgactttcc tagcctttgc cgacgattgc agctcttata cattggacaa cctgacgttt
     1681 gaaaacaatg ccgatcgcct ctctgtctat ggtgacatgc agattcaacg cgaccaacaa
     1741 ggcttgcagc aattactggc cttacaaagc ctcatcaatc aagctgtcaa ggctttacag
     1801 gccgatgnnn nnnnnnntga tacactgcca gagcagctgc ctgcagccaa gcacaatgaa
     1861 atcgacaacc cgtttctgcc ttaacccctc agttttactg ccatccaagt gaagcatcag
     1921 cagtagcggc ctgtaagcca gcagcacctt acaggccgct gctcgatact tgccacattc
     1981 aatagcaaac actctatact gggcggacag ctgactttga gaattgagaa agtaagatga
     2041 aaccattgat aaaaccattg tggctagcaa gctgcctctt ggctttgagc acatctgttt
     2101 gggcagcacc ccgtttgatt tcagccgagc ccaatactga gcattattcg ggtgccgatg
     2161 tggatcaaac catcatcctc aaaaaccaag acatgaacgc caaagtcttt tcttacagcg
     2221 gtggcgatcc tgccatcaat ggcgcctacc tgaatttggc cgtgtacgat gaagataaac
     2281 ggggttggaa tgtgtttgtc ttgaaagacg tgcttgacta taaagtcttg cccagcgccc
     2341 aacaaggcta tgccaaaatt gccttgatca cccaagatgt cgaccaacgc agcggcaaca
     2401 tacgttcata taaatccacg ctgttcatca atttgcgcaa tgccaaccaa cgcggcggga
     2461 aaattgaagt cgacgaaatc aagcaaaaat aacgtatccg atgaatggtt acaagactct
     2521 caataagtct ttaaatcgcc tccctcacca cagcggcctg taaacactga tgcttacagg
     2581 ccgcttattt catgagctgc aaggttgtat caatgtcacc atgggtccca aaaaatggcg
     2641 catgtcttcc ctgcctgttt gcggcaaata caggctggaa attgagccat ccaaatacaa
     2701 ggcttgttcg gtttttaaag tgtctttgaa cacttgagca aactggtaaa aactcacagg
     2761 cttttggctg atggcaaaat acagcgtttt gcctttaatg cccacgccat tgcggatttt
     2821 tttagacgtg gcatcgggta aaaactgcgg attgatttga tgattgatga ccaacattgg
     2881 tccagactgg gtggcataag cggcctgtaa attttgccca gcatagtctg ccgttttgcc
     2941 tatccaagcc tgctcggcat tccaggccaa cacaccattg ggctgcatga agaaattgcc
     3001 aaaacctgta tcagtattga gcggcgtgag ctgtttgcct tgcgccacat acaggccaac
     3061 aggctggtaa ttcgcatgaa acatgcctgc attcatacca aacaacatgc gctcacacgc
     3121 aggcaactcg ccacgcaccg ccgcaaacga ctggtaagcg tatccgcccg agttgttcaa
     3181 atacagacgc aaatcggcca gtttttccac tttgatgaca tcgatggcca caccattatt
     3241 ggcatgcact gtctgcacag ccacttcgga tgcattgcat gcgctcaaac ccatgctgaa
     3301 acacaacacc aataaaaccg attgccatgc ttgcttcatc gtctttacca cctttgctta
     3361 atacagattg tttatagcat cacaaaaccc atcacagttt tacaaactcg ccacgccatt
     3421 gctcgccacc accgccatca cccactcttc aaacgtgcgc cagttggcga tttcggcata
     3481 ttcatccaca ggtgccgcgt ggtcgtacac atcaaaacaa cgctcatctg cccgcgccaa
     3541 attaaaaccc atccaatcgc ccatggattg gtgcacgccc accatcagat acgcatcatc
     3601 catgttgatg ccttcatcca gcaaatgctt ggccagaaag cgctggtttt gcctcatttc
     3661 atccgcaccg tacacgcaaa acatgccttg atgggcatgc ctcaggaaaa actcacccac
     3721 gccgatttgg cgaataaact cataatacgc agcaggcaag cgcatgtctg ttgcctctgc
     3781 aatttcaacc cactctgctt cactgagggt gcgcactgca aagcgctgct gctcggtatc
     3841 gctggctata caatgccgca aggtatgcag cgcccgttgg taatctgttg ccatattgct
     3901 cgtcctcagt ctgtgtgcac atgatagcaa aaaccataaa aaaagcggcc tgtaaggttt
     3961 acaggccgct tattcaaggt ttcaagctgc gattaagcca acaaacgctc ttcgcccttg
     4021 ccttcgacat tgtctatgca acgtgcgcac aaacatgggt gggcgctgtt gctgcccacg
     4081 tctggcaagt aatgccagca acgctcgcat ttttcacctt cggctttgtt gacaacaatg
     4141 ctcaaggctt cgccttcgct gacgctgact ttcgacacca tcaacacaaa gcgcaattct
     4201 tcgcccaaag cacgcaaata gccgtaagtt ggctcaggtg cagtgatgct caccgccgct
     4261 tgccatgatg aacccaccac ttcggcagcg cgcttggttt cgatttcctt gttcaccgct
     4321 tcgcgcactt cgcgcacggc ttgccatttt ttcgccaatt ccgcttccac ggtttcaggc
     4381 atggatggga attcgtgcaa agtgtggtag agcacggagt cttcgttgcc gcctgccaag
     4441 atttcccac
//
LOCUS       DAOMVK010000152         4444 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_5616_length_4444_cov_3.175211, whole genome shotgun sequence.
ACCESSION   DAOMVK010000152 DAOMVK010000000
VERSION     DAOMVK010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5616_length_4444_cov_3.175211"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PK856_06130"
     CDS             <1..377
                     /locus_tag="PK856_06130"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRL01983.1"
                     /translation="IMNTYYVGLMANSTDPEEKKIGESIGVFFPNQATTGTHINISGA
                     GLTKSSKNKAHATQLIEFLTDVPAQEMVSNENYEFPVNPKAQPAELLRSWGTFKTQQI
                     NFAELGKNNAAAIALMGKTGWK"
     gene            381..1997
                     /locus_tag="PK856_06135"
     CDS             381..1997
                     /locus_tag="PK856_06135"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRL01984.1"
                     /translation="MLKKSNSLWIMLSQIGGVIVLLPFVFLCVNLFVPSNENWNTLSQ
                     YWLKEYITNTLILIASVGVIVASLGSILAFLMCYYRFPLKKFFEFFLVLPLAIPAYIS
                     AYTYSEMLSYTGVIQKNLRNVFGLNLPSNYFDIMTLPGAIFIFSMALFPYVYLMVRSF
                     LIRQDAGLMESARLLGKNVFSAYLTVILPMTLPVMVTSATLACLEVLSDYGVTSHFGI
                     HTFSTGIFQAWFGMHDVFTAIKVAFCGMVIATSLIVINRVFTNTKKIQAQANTKPIQP
                     MPLTGVGLWFGLGALLLVSLLSSIVPIAQMLVWAVQTLMHQNELQGLFENTKNTLFVA
                     TIASVAVILFSLIVCNVGRYFSAFWAKLPMWVGGFGYSFPSPVLAIGVLGLLISVDKW
                     LFSMKLADNALLLTSTLFTLVFAYVIKYAAVGINGIDSGFTRVGNRYSEASRTLGHGM
                     TATFFKVDVFTIKHSIIAAFILVFIDIVKELPMTLLLRPYNFSTLATRVYTYAGDEQI
                     QRASVPSLVIIFICVVLMFVVNKIEKNRHY"
     gene            1999..2628
                     /locus_tag="PK856_06140"
     CDS             1999..2628
                     /locus_tag="PK856_06140"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL01985.1"
                     /translation="MYLTLENIGFSYQKSDKPTLKHFNLAISKGASIGLVGASGCGKS
                     TLLRLIAGLETLAEGSITLDAQLLSSPKVCVLPEKRQIGMVFQNYCLFPHLTVRKNIA
                     YGLRQRLHKKERVNEMLALIEMEDHADRYPYQLSGGQQQRVALARSLAPKPKIILMDE
                     PFSNLDAALKKSVRDEVKSILKAENMTSIIVSHDVQDTDALCDDTVVLG"
     gene            complement(2761..3996)
                     /gene="alr"
                     /locus_tag="PK856_06145"
     CDS             complement(2761..3996)
                     /gene="alr"
                     /locus_tag="PK856_06145"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF009879.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRL01986.1"
                     /translation="MQTKMKSLALMLTLALGMPAISQAAPLLADQGEPAAKLARVPQA
                     NAWLEIDKQAFTDNIRTLQKELDGKSKICAVMKADAYGNSIDLLMPSILETNVECVGI
                     TSNAEAAIVRQHGYKGRIARLRAATTNEILDALPLNVEELFGNLEQAKSISAQMKSRN
                     HTLKYHLALNAGGMDRNGLEMVTPKGKQQAVELSKLPNLKMVGIMTHYAVEDEKFVRD
                     HLKIFLEQTDWLIKTAKLKREDLTLHTANSFTTLAVPEARLDMVRPGGVLYGDSIPSY
                     TQYKKTMAFKTQVAVVNSYLKGTTVGYDETYTLKRDSRLANLPFGYSDGYRRVFSNKG
                     YVLINGHKVPVVGKVSMNTTMVDVTDFPDIKAGDAVVVYGKQGNEEVKQADVEEYNGA
                     LLADLYTVWGNSNQRKLKQ"
     gene            complement(4154..>4444)
                     /locus_tag="PK856_06150"
     CDS             complement(4154..>4444)
                     /locus_tag="PK856_06150"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003773793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRL01987.1"
                     /translation="LPRHPSQLYQFALEGLLLFVILWVFAKKQRPVGQISAVFLMGYG
                     AFRFIAEFAREPDAQLGLLGMGLSMGQWLSLPMVLAGALLFVYCQKRAQPTV"
BASE COUNT         1087 a          984 c         1075 g         1298 t
ORIGIN      
        1 ccatcatgaa tacctattat gtgggcttga tggcaaattc taccgaccct gaagaaaaga
       61 aaatcggtga aagcataggc gtgttcttcc cgaaccaagc caccacaggc acacacatca
      121 acatcagtgg tgcaggcttg accaaatcga gcaaaaacaa agcgcatgcc acccaattga
      181 ttgaattctt aaccgatgtg ccagcgcaag agatggtttc caatgaaaac tatgaattcc
      241 ctgtaaatcc caaagcccag cctgctgaat tgttgcgctc ttggggcacg ttcaaaaccc
      301 aacaaatcaa ttttgctgaa ttgggcaaga acaatgcggc ggcgattgcc ttgatgggta
      361 aaactggctg gaaataatgc atgctgaaaa aatccaatag tctttggatc atgctcagtc
      421 aaatcggagg agtcatcgta ctcctccctt ttgtattttt gtgtgtgaat ctgtttgtgc
      481 cttccaatga aaattggaac actttgtcac agtattggtt gaaagaatac atcaccaata
      541 cgcttatttt gatagcaagt gtgggcgtga ttgtggcctc tttaggcagc atattggcgt
      601 ttttaatgtg ttattaccgc tttccattga agaaattttt tgaatttttc ttggtattgc
      661 ctttggcgat acctgcttac atttccgcct atacctacag cgaaatgctc agctacacag
      721 gtgtcataca aaagaactta cgcaatgttt ttggtctgaa tctgccttca aactatttcg
      781 acatcatgac tttgcctggg gcgattttta tattttcaat ggcattgttc ccctatgtgt
      841 atttgatggt acgcagtttt ttgattaggc aagacgcggg tttgatggaa agtgcacgat
      901 tgctgggtaa gaatgtgttt tcggcttacc tcaccgtcat tttgccgatg actttgccag
      961 tgatggtgac cagtgctacc ttggcttgct tagaggtgtt gagtgattat ggggtcacca
     1021 gtcattttgg tattcatacg ttttcaacgg gcattttcca agcgtggttt ggcatgcacg
     1081 atgtctttac cgccatcaag gtggcgtttt gcggcatggt catcgccacc agtttaatcg
     1141 tcatcaatcg ggtgttcacc aataccaaga agattcaagc tcaggccaat accaagccga
     1201 ttcagcccat gccattaaca ggtgtgggtt tgtggtttgg tcttggtgcg ttgttattgg
     1261 tgagtttgct ttcaagcatt gtgcctatcg ctcagatgtt ggtttgggcc gtgcaaacgt
     1321 tgatgcacca aaatgaactg caaggtttgt ttgaaaatac caaaaacacc cttttcgttg
     1381 ctacgattgc ttcggttgcc gtcattcttt tttcactcat tgtgtgcaat gtagggcgct
     1441 atttttctgc tttttgggcg aaattgccga tgtgggtggg tggttttggc tattcatttc
     1501 catcgcctgt tttggccatc ggtgttttgg gcttgttaat ctcggtggac aaatggctgt
     1561 tcagcatgaa attggctgac aatgcgctgt tgttaacttc gacactgttc actttggttt
     1621 ttgcctatgt gattaagtat gcggcggttg gcatcaatgg catagacagc ggtttcaccc
     1681 gcgtcggcaa tcgctattct gaagcatcgc gaacgctggg ccatggcatg acggcgactt
     1741 ttttcaaagt cgatgttttt accatcaaac actcaatcat cgctgctttt attttggtgt
     1801 ttatcgacat tgtgaaagag ctgccgatga ctttgttgct caggccgtat aatttctcca
     1861 ccttggcaac cagagtctat acctacgcgg gcgatgagca aattcagcgg gcatcggtgc
     1921 catcattggt cattatcttc atctgtgttg tgttgatgtt tgtggtgaac aaaattgaaa
     1981 agaacaggca ttattgacat gtatctcact cttgaaaata tcggcttttc gtatcaaaaa
     2041 agtgacaagc ccaccttaaa acactttaac cttgccatta gcaagggagc atccatcggt
     2101 ttggtggggg cgagtggctg cggaaaaagc actttactgc gtttgattgc aggtttggaa
     2161 actttggcag agggcagcat caccttggat gcgcaattgc tgtcttctcc taaggtgtgt
     2221 gttttgcctg aaaagcggca aattggcatg gtgtttcaaa actattgctt gtttccgcat
     2281 ttaacggtgc gaaagaacat tgcctatggc ctacgccaac ggctgcacaa aaaagaacgc
     2341 gtgaatgaaa tgctggcatt gatagaaatg gaagaccatg ccgaccgtta cccgtatcaa
     2401 ctcagtggcg gccagcaaca gcgcgtggcc ttggcaaggt ctttggcacc caagccgaaa
     2461 atcatcttga tggatgagcc tttcagtaat cttgatgccg ctttgaaaaa atcggtgcgt
     2521 gatgaagtga aatccatttt gaaagctgaa aacatgacca gcatcattgt gtcccatgat
     2581 gtgcaagaca ccgatgcatt gtgcgatgac acagtggtat tgggataagc agtattcatg
     2641 gtttagcagt ttaagttgtg ttcatgagat agcaaacaaa gcggcctgta aaaccaatat
     2701 tgaatttaca ggccgcttgt ttgcatcaaa tcacactatt gcatctacat aacatcatta
     2761 ttactgtttt aatttacgct gattcgagtt gccccatacg gtgtacaaat cggcgagcag
     2821 tgcgccattg tattcttcca catccgcttg ttttacctct tcattgcctt gcttgccgta
     2881 caccaccacc gcatcgcccg ctttgatgtc agggaagtcg gtgacgtcta ccatggtggt
     2941 gttcattgac actttgccca ccacagggac tttgtggcca ttaatcaaga catagccttt
     3001 gttactgaac acgcggcggt agccatcgga ataaccaaac ggcaaattgg ccaagcgcga
     3061 atcgcgtttt aaggtgtagg tttcgtcata gccaacggtg gtgcctttaa gatagctgtt
     3121 gaccacggca acttgggttt tgaaggccat ggtttttttg tattgggtgt agcttgggat
     3181 gctgtcaccg tataacacgc cacctgggcg caccatgtct agacgtgctt caggcacggc
     3241 caaggtggtg aaggagttgg cggtgtgtag ggtcaagtct tcgcgtttga gcttggcggt
     3301 tttgatgagc cagtcggttt gttctaagaa aattttcaaa tggtcgcgca cgaatttctc
     3361 gtcttcaaca gcgtaatggg tcatgatgcc aaccattttc aagttcggta gcttggacaa
     3421 ttccaccgct tgctgtttgc ccttaggcgt caccatttcc aaaccattgc ggtccatgcc
     3481 gcctgcgttc aaggccaaat ggtattttaa ggtgtggttg cgagatttca tttgtgcgga
     3541 aatggatttg gcttgttcta aattgccaaa caattcttcc acattcaaag gcaaggcatc
     3601 caagatttca ttggtggtgg cagcgcgcaa gcgagcgatg cggcctttat agccgtgttg
     3661 acgcacgatg gcggcttcgg cattgctggt gatgcccaca cattcgacat tggtttccaa
     3721 gatggatggc atcaataaat caatgctgtt gccataggca tcggctttca tcaccgcgca
     3781 gattttggat ttgccatcca attctttttg caaagtgcgg atattatcgg taaacgcttg
     3841 cttgtcgatt tccaaccacg catttgcttg cggcacacgc gccaatttgg cggctggttc
     3901 gccttggtcg gccaataaag gtgcggcttg gcttatggca ggcatgccca gcgcgagcgt
     3961 caacatcaat gccaatgatt tcatcttggt ttgcatggtt tctccgttcg ttttctctgt
     4021 tctttgaatg taataatacc gccactgagg gcggtaccaa ggattgcgga taggtttaca
     4081 ggccgcttat attgcttggt gtgtcgatat tagcttaata cagccagaat atgaaacacc
     4141 aagttgtcat tttttacaca gtaggctgcg ctcgcttttg gcaatacaca aacaacagcg
     4201 cgccagccaa gaccatgggc aggctcaacc attgacccat ggacaaaccc atgcccaata
     4261 agccaagttg ggcatcgggt tcgcgtgcaa attcggcgat gaagcggaat gcaccataac
     4321 ccatcaagaa cacagcagag atttgaccga caggacgttg tttcttggca aacacccaca
     4381 aaatcacaaa caacaacaag ccttctaaag caaattggta taactgtgac ggatgacggg
     4441 gcaa
//
LOCUS       DAOMVK010000153         4422 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_5654_length_4422_cov_3.546828, whole genome shotgun sequence.
ACCESSION   DAOMVK010000153 DAOMVK010000000
VERSION     DAOMVK010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5654_length_4422_cov_3.546828"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..92)
                     /locus_tag="PK856_06155"
     CDS             complement(<1..92)
                     /locus_tag="PK856_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HRL01988.1"
                     /translation="MGIDGVLEKGFITTSADTVLNYMRTGSMWPV"
     gene            complement(83..439)
                     /locus_tag="PK856_06160"
     CDS             complement(83..439)
                     /locus_tag="PK856_06160"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HRL01989.1"
                     /translation="MLANYFPVLLFIIIGLVAGGAFITLGSLLAPSRPYEEKNSPYEC
                     GFEAFENARMRFDVRYYLVAILFILFDLEIAFMFPWAVVFKELGAFGFWSMFIFLVIL
                     TIGFVYEWKKGALEWE"
     gene            complement(803..888)
                     /locus_tag="PK856_06165"
     tRNA            complement(803..888)
                     /locus_tag="PK856_06165"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(852..854),aa:Leu,seq:gag)
     gene            complement(895..1248)
                     /gene="secG"
                     /locus_tag="PK856_06170"
     CDS             complement(895..1248)
                     /gene="secG"
                     /locus_tag="PK856_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003824253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRL01990.1"
                     /translation="MEALKTVVLLVNIFSALAVIVLVLLQHGKGADAGAAFGSGSAQG
                     VFGAAGSANFLSRGTALAATIFFATCLAMGWFAAHQKSGLNFDQAQPAVQQTAPAQTA
                     PVAPKPAAASQVLPE"
     gene            complement(1253..2005)
                     /gene="tpiA"
                     /locus_tag="PK856_06175"
     CDS             complement(1253..2005)
                     /gene="tpiA"
                     /locus_tag="PK856_06175"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRL01991.1"
                     /translation="MTTQKWVLGNWKMNGRLAANQALLSQLSQIPANEQVAVGVAVPS
                     VYISSALQAASDTLIGAEDVSAYANDGAYTGEVSASMLADVGAQFVLIGHSERRQYFA
                     EGESVLAQKYAHAVAVGVLPVLCVGETLAEREAGQAEAVVAKQLALLQAAPLLNQTVL
                     VAYEPVWAIGTGVVASIEQIAQMHAFIQSHVLSLLNNGATIRLLYGGSVNADNCEAIF
                     AVKHVDGALVGGASLKFDSFAQIITAAVSAAN"
     gene            2214..3329
                     /gene="pstS"
                     /locus_tag="PK856_06180"
     CDS             2214..3329
                     /gene="pstS"
                     /locus_tag="PK856_06180"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HRL01992.1"
                     /translation="MRLLLSSAIVLGALGLAACGGESSKPVTPANSSASGSSAKPATG
                     GATGITGAGASFPQPIYAQWAQDYNAAKGSQVNYQSIGSSGGVKQINAKTVDFGATDA
                     PLSKEDLDKNGLIQFPTVIGGVVPIVNIDGIEPGKLKLDGATLANIYLGKITKWSDPA
                     IKALNPDVTLPDAAITTVFRSDGSGTSFNFTNYLSAVSPEWKEKVGAANSVKWPTATG
                     TAGTAGKGNEGVANFVRTVKNSIGYVEYAYAKQNNMSHVSLKNKAGNFVQPSLDTFSA
                     AAKADWNSAPGFGLILTDQADANAWPIASATFILVHTKPQDAAKAKATLSFFDWVYNS
                     GDEAASKLDYVPLPADVKALVRESWKQVVDAEGKPVL"
     gene            3418..4368
                     /gene="pstC"
                     /locus_tag="PK856_06185"
     CDS             3418..4368
                     /gene="pstC"
                     /locus_tag="PK856_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HRL01993.1"
                     /translation="MTQLQQKLATQRKLDWLFVSLTKFFAFLVLASLVGIMITLIMGA
                     IPSMQHFGWGFLTSKNWDPVAGEYGALAPVFGTIVTSVIALLVAVPVSFGIAIFLTEL
                     SPAWLKRPLGIAVELLAGVPSIIYGMWGLFVFAPFFGEHIQPHMIKYFGNIPMIGTLF
                     QGAPMGIGLFTAALILAIMIIPFIASVMRDVFEVTPTLLKESAYGLGSTTWEVVRHVV
                     LPYTKAGVVGGIILGLGRALGETMAVTFVIGNTFNITGSLFSSGVSITSALANEFGEA
                     VDEAHVSSLLFLGLILFIITFIVLCISKIMLLRMQRHEGR"
BASE COUNT         1249 a         1091 c          910 g         1172 t
ORIGIN      
        1 acaggccaca tagagccagt acgcatgtaa tttaaaacgg tatcagcact cgtggtgatg
       61 aaaccctttt ctaaaacgcc atctattccc attccaaagc gccttttttc cactcataaa
      121 caaagccaat tgtcaggatg accaagaaaa tgaacattga ccaaaagcca aatgcaccca
      181 attccttgaa cacaacagcc caaggaaaca tgaaggcgat ttccaaatca aacaaaataa
      241 acaaaatggc cacgaggtaa taacgcacat caaaacgcat acgggcgttc tcaaacgcct
      301 caaacccgca ctcataggga gagtttttct cttcatatgg acgactcggt gccaacaaag
      361 accccaaggt gatgaatgcc ccaccggcta ccagcccgat gataataaac agtaatactg
      421 gaaagtaatt cgctagcatg gctgaacgac ctaaacgaaa ttcaagataa tcccatctat
      481 tgtagccaat taaatcagtg atttaaagtc ttgtattttt gtacaattta ccatcactaa
      541 caatttctgc ttaaataata gtctatcctg acaaattatt aaaattgcct aaaaaaatct
      601 caaacgaatt catcatgtta ataagttatt gataatgatt attattttat ttattgttta
      661 gaattttctg gtttttttta gagctgccaa ataaaaaata acaatttaac gttttttggc
      721 ctccaatcaa aagacttttg atttgcatca gctttttaag aacataaaaa aaacagcccc
      781 attgggactg tttttaaatt tttggtgccg acaatgagac ttgaactcat acgacctagg
      841 gccaccaccc cctcaagatg gcgtgtctac caatttcacc atgtcggcaa tgtactactc
      901 aggcaatact tgactcgctg ctgcaggttt aggggccact ggagcagttt gggcaggtgc
      961 agtttgttgc accgcaggtt gagcttgatc aaaatttaaa ccactctttt gatgagctgc
     1021 aaaccaaccc atggccaagc aagttgcaaa gaagatggtt gcagccaaag cggttcctcg
     1081 actcaaaaag ttagctgaac ctgctgcacc aaacacacct tgagcactgc cggagccaaa
     1141 tgcagcacct gcatcagcac ctttaccgtg ttgcaacaat accaacacaa taacagccaa
     1201 ggctgaaaaa atattaacca ataaaacgac ggtttttaaa gcttccatag catcaattcg
     1261 cagcactaac ggccgctgta ataatttggg caaagctatc aaacttcaga gatgcgccac
     1321 ctaccaatgc accatccaca tgtttcacag caaatatggc ttcgcagttg tcagcgttga
     1381 cactgccgcc gtaaagaagc cgaatagtag caccgttgtt taacagtgac aagacatgtg
     1441 attgaataaa ggcatgcatt tgtgcaattt gctctatgct cgccacaaca cctgtgccta
     1501 tcgcccaaac aggctcataa gccaccaaca ccgtttgatt caataaagga gcggcctgta
     1561 acaaagccaa ttgtttggcg acaacagctt cagcctgacc tgcctcacgt tcggccaagg
     1621 tttcacccac gcacagcact ggtaacacgc ccacagcaac cgcatgggca tatttttgcg
     1681 ccagcacgct ttcgccttct gcaaaatact gacgacgctc tgagtgacct atcaacacaa
     1741 attgtgcacc cacatcagcc aacatagatg cagacacttc tccggtatac gcaccatcat
     1801 tggcataagc actgacgtct tcagcaccaa ttaaagtatc agaagcagcc tgtaaagcac
     1861 tgctgatgta gacagaaggc actgccacac caacggcaac ttgctcattt gctgggattt
     1921 gactcaattg tgacaacaaa gcctgattgg cagccaaacg gccattcatt ttccaatttc
     1981 ccaataccca tttttgcgtt gtcatgcgtg tccttaatat ctgaattcgc ccgcattgta
     2041 ctgaagtgca agataaacag tccagttgta tgtgtgtcag tggattaaaa gattttgtca
     2101 caatggcagg cattgtcaca cagttgacac aattgcctct tacactgcga aacatcaaat
     2161 ttaattgtta caactttaac agtttttcta tacacactaa ttggagtttt accatgcgtt
     2221 tattgttatc ttcagccatt gttttgggtg ccttaggctt ggcagcttgt ggtggcgaat
     2281 catcaaaacc tgttacccca gccaacagca gcgcttcagg ctcttctgca aaacctgcta
     2341 ctggtggcgc aactggcatc acgggggcgg gtgcatcttt cccacagccc atttatgcac
     2401 aatgggcgca agactacaat gcagccaaag gctcacaagt taactaccaa tccatcggtt
     2461 ctagcggcgg tgtcaaacaa atcaatgcca aaacagtaga tttcggtgcc acagatgcac
     2521 ctttgtctaa agaagacttg gataaaaatg gtttgattca attcccaact gtgattggtg
     2581 gcgtggttcc catcgtgaac attgatggca ttgagcctgg caaattgaaa ttggatggag
     2641 caaccttagc caacatttac ttggggaaaa tcaccaaatg gtctgatcca gccatcaaag
     2701 ccttgaaccc tgatgtgacc ttaccagatg cggccattac cactgtattc cgctctgatg
     2761 gctctggtac cagtttcaac ttcaccaatt atttaagtgc ggtttctcct gaatggaaag
     2821 aaaaagtagg tgctgccaac tcagtaaaat ggcctacagc cacaggcacg gctggcacgg
     2881 ctggcaaagg caatgaaggc gtggccaact ttgttcgcac ggtgaaaaac tccattggtt
     2941 atgttgagta tgcctatgcc aaacaaaaca acatgtcaca tgtgtcttta aaaaacaaag
     3001 caggtaattt tgttcaacct tctttggata ccttctctgc tgctgccaag gcagactgga
     3061 attcagcacc tggcttcggt ttgattttga ctgaccaagc cgatgccaat gcatggccta
     3121 tcgcttccgc caccttcatc ttggtacaca ccaaacctca agatgccgcc aaagccaaag
     3181 ccactttaag cttctttgac tgggtttata acagcggtga cgaagctgcc agcaaattgg
     3241 actatgtgcc tttgccagca gatgtcaaag ccttagtgcg tgaaagctgg aaacaagtag
     3301 tggatgcaga aggcaaaccc gtcctgtaaa ccttgaaacc acgccgctgc ttgccctttt
     3361 tgggtgacag cggtgttgtt ttacgcagcg atacagcacg ctaaatagag aatacttatg
     3421 actcaattac agcaaaaact ggcaacccaa cgcaagctgg actggttatt tgtcagcctg
     3481 accaaattct tcgctttctt ggtgttggca agtttggtgg gtatcatgat taccttaatc
     3541 atgggcgcca tacccagcat gcaacatttc ggctggggct tcttaaccag caaaaactgg
     3601 gatcccgtag caggtgaata cggtgcattg gctcccgtat tcggaaccat cgtcacctct
     3661 gtcattgcct tactcgtagc agtgcctgtg agttttggta ttgccatctt cttaaccgaa
     3721 ttaagcccag cttggctcaa acgcccattg gggattgcgg ttgaattact ggccggtgtc
     3781 ccatcgatta tctatggcat gtggggcttg tttgtgtttg cacctttttt tggcgaacac
     3841 atccaaccac acatgatcaa gtattttggc aacattccta tgattggcac cttatttcaa
     3901 ggcgcaccca tgggcatagg cttgttcacc gcagccttaa ttttggccat catgatcatt
     3961 ccctttatcg cctcggtgat gcgtgacgta tttgaagtga cgccgacttt attgaaagag
     4021 tctgcctacg gtctgggctc aaccacttgg gaagtggtgc gccatgtggt tctgccctac
     4081 accaaagccg gtgtggtggg tggcatcatt ttgggtttag gccgcgcctt aggtgaaacc
     4141 atggcggtga cttttgtcat tggcaacacc ttcaacatta caggtagctt gttcagctca
     4201 ggcgtctcca ttacttctgc cttagccaat gaatttggtg aagccgttga cgaagcccac
     4261 gtgtcttctt tgctgttctt gggcctcatc ctattcatca ttaccttcat tgttttatgc
     4321 atttcaaaaa tcatgttgtt gcgcatgcaa cgtcatgaag gccgctaatt aaggaaacaa
     4381 catgaataac gccttataca aacgccgctt actgattaac aa
//
LOCUS       DAOMVK010000154         4418 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_5664_length_4418_cov_2.656429, whole genome shotgun sequence.
ACCESSION   DAOMVK010000154 DAOMVK010000000
VERSION     DAOMVK010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5664_length_4418_cov_2.656429"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="PK856_06190"
     CDS             complement(<1..866)
                     /locus_tag="PK856_06190"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRL01994.1"
                     /translation="MKLLNSKKTRAYMMLAPLILFVVVFFLWPLLVMMKQSISDDAVS
                     SVLKQTASVMHEWDEKSPPTAAMQQAFIKDIQTEQDPQAIGNMIRKLNSEQAGFRTLL
                     TKTSTKINGTPTVTDLVSIDERWSDPKYWLAINKLLSPVTDRYLLAAVDMNRDLKGDI
                     QKMPASQSANLDIMLRTFWIAALVTIICVLIGYPYAILLASSSGWVKNVLFMAVLLPL
                     WTSLLVRTAAWFILLQDQGLINDALQGIGLINEPLHLIFNRVGVVISMTHVLLPFMVL
                     PIYSVLKTIPGN"
     gene            complement(863..1936)
                     /locus_tag="PK856_06195"
     CDS             complement(863..1936)
                     /locus_tag="PK856_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL01995.1"
                     /translation="MEKNAHIRFDNISKFYGDVKIIEDLDLNIKKGEFISLLGPSGSG
                     KTTLLMMLAGFESATKGTVWLNDKSLNDIPSYKRNIGVVFQNYALFPHMTVAQNIGFP
                     LQMRGVSKADIKQKVEKVLDMVQLAAMKERKPAQLSGGQQQRVALARALVFEPEVILM
                     DEPLGALDKQLREQMQLDIRALHQRLGVTIVFVTHDQEEALTMSDRIAVFNKGNIEQI
                     ASPREIYDQPKTKFVAEFIGATNLIEGKIIALQADKAQVQLSSQQNIWVQAAQDVAVN
                     DEVYVSVRPEKVNLTQQSGAYDNQFPTTIKDIVYLGNHLRVQLDENAHHFIVNQDRKV
                     VEWAKGAAVVAGFNAADCWMIKR"
     gene            complement(2008..3123)
                     /locus_tag="PK856_06200"
     CDS             complement(2008..3123)
                     /locus_tag="PK856_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRL01996.1"
                     /translation="MNITRLALIMGTLAITACGGGSNTTPASNTPAASNAPASAAPAA
                     SSGSRDLTIASWGGNYQDAQKKIYFKPFSEKLGKPVLDENYDGGYGVIQAKVQGGNPN
                     WDVVQIESEDLARACTDGLLEKLDWNKLGGKDKFIDGAAGDCGVGAIVWSTAIAYDGD
                     KLKTAPQSWADFWDTKKFPGKRSLRKGAKYSLEFALLADGVKKEELYQVLGTPEGVDR
                     AFKKLDELKPNIVWWEAGAQPLQFLASGEVVMASAYNGRIAGLNRTEGKNFKVVWPQS
                     IYAIDSWAILKGAANKDAGMDFIAFASLPENQAKLPEFVAYGLPNKEAATKVPEKFAA
                     ELPTTAANMTDSIPLNVDFWIDHSEELTERFNAWLAK"
     assembly_gap    3201..3210
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3354..>4418)
                     /locus_tag="PK856_06205"
     CDS             complement(3354..>4418)
                     /locus_tag="PK856_06205"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRL01997.1"
                     /translation="AMTAQASTLCTHTRYLHDGILTYADRLLARFPSYLNRLMLTCTG
                     SEANDLAIRIAEAVTGGTGVVVTSNAYHGVSHLLAKLSPSLAPIDDFVRVVAPPRAAA
                     DEAGNATEAKRFAVDVTKAFEDLQAAGYQPCALLMDMIFASDGILPPSAGLLNEAAKA
                     TKKMGAVVIADEVQSGFGRTGDNWWGFVDANLQPDIVTMGKPMGNGYPIAGVVAGDDL
                     VATFAAKGRYFNTFGGNPVAVATGMAVLDELEERQLPAKAEELGLGIQAAFAQELTGL
                     GTVDIRGRGLYWGIEIAADGKETATERATRVTNHMRQNGVLLSNSGPDAKVLKIRPPL
                     VFEQEHAQMLVAALKQALQA"
BASE COUNT         1100 a         1174 c          992 g         1142 t
ORIGIN      
        1 aagttgccag gtatggtttt caacacgctg taaatcggca acaccataaa aggcaacaac
       61 acatgtgtca tcgaaatcac cacaccgaca cggttgaaaa tcaggtgtag cggctcatta
      121 atcaagccta tgccttgcaa ggcatcgtta atcaagcctt ggtcttgcaa caagataaac
      181 caagcagcgg tacgcacaag cagcgatgtc cacaaaggca gcaacaccgc catgaacaac
      241 acgtttttca cccaaccact gctggacgcc aacaaaatcg catacggata accaatcaac
      301 acacaaatga tggttaccaa agcggctatc caaaacgtgc gcagcataat gtccaagttg
      361 gcagactgtg aagcgggcat tttttgtatg tcgcctttta aatcacggtt catgtccaca
      421 gcggccagta aataccgatc ggtcacaggt gagagcaatt tgttgatggc cagccagtat
      481 ttcgggtcag accaacgctc atcaatgctc accaagtcgg tcacagtcgg agtgccgttg
      541 attttggttg aggttttggt caacaaagtc cgaaagccag cttgctcact gttgagcttg
      601 cgtatcatgt taccgatggc ttgtgggtct tgctcggttt gtatgtcttt gataaacgct
      661 tgttgcatcg cggcggtggg cggtgatttt tcatcccact catgcatcac gctggcggtt
      721 tgttttaaca cgctggacac cgcatcgtca gaaatggatt gcttcatcat caccaacaaa
      781 ggccacagaa aaaacaccac cacaaacaaa atcaatggcg ccagcatcat atacgcacgg
      841 gtctttttgc tattcagtag tttcatcgct tgatcatcca acagtctgca gcattgaacc
      901 cagccaccac agcggccccc ttagcccact ccaccacttt gcggtcttga ttgacgatga
      961 aatggtgggc attttcatcc agttgtaccc gtaagtggtt gcccaaatac acgatgtctt
     1021 tgatggtggt tggaaactga ttgtcgtaag cgccactttg ttgggtcaaa ttgacttttt
     1081 caggccgcac cgacacatat acctcatcgt tcactgccac atcttgagcg gcctgtaccc
     1141 aaatattttg ctgactggac agctgcactt gggccttatc ggcctgtaaa gcaataatct
     1201 tgccttcaat caaattggtc gcgccgatga attcggccac aaatttggtt ttcggttggt
     1261 cgtaaatttc gcgcggactg gcgatttgtt cgatattgcc tttgttgaac acggcaatgc
     1321 ggtcagacat ggtcaaggct tcttcttgat catgggtcac aaacacgatg gtcacgccca
     1381 aacgctggtg caaagcacgg atgtccaatt gcatttgttc gcgcaattgc ttgtccaaag
     1441 cgcccaatgg ttcgtccatc aaaatcactt ctggctcaaa caccaaagca cgcgccaagg
     1501 ccacacgctg ttgctgacca cccgacaatt gggcgggctt gcgctctttc atcgccgcca
     1561 attgcaccat gtccaagact ttttcaactt tttgtttgat gtcggccttg ctcacaccgc
     1621 gcatttgcaa tgggaaaccg atgttttgcg cgaccgtcat gtgtgggaac aatgcatagt
     1681 tttgaaacac cacaccgatg ttgcgcttgt aggaagggat gtcgtttaat gatttatcgt
     1741 ttaaccatac cgtgcccttg gtggccgact cgaagcctgc caacatcatc aacaatgtgg
     1801 ttttgcctga accagatggg cccaataagc tgatgaattc gcctttttta atattgagat
     1861 ccaaatcctc aatgattttg acatcgccgt aaaatttgct gatgttgtcg aaacgaatgt
     1921 gagcgttttt ttccaatgtg attccccttc tttttatcgc tgctgcaaaa aggttccacc
     1981 tgcaatacag gtggaacgct tcttcactta cttggccaac caggcattaa aacgctcagt
     2041 caattcttca gagtggtcga tccagaagtc cacattcaaa ggaatcgaat cagtcatgtt
     2101 agctgccgtt gttggcaatt cagccgcaaa tttctcaggc actttggtgg cggcttcttt
     2161 gtttggcaat ccataagcca caaattctgg caatttggct tggttctcag gcaagctggc
     2221 aaacgcgata aagtccatgc cagcatcttt gttggcagcg cctttcaaga tggcccagct
     2281 gtcgatagcg taaatgcttt gtggccaaac cactttgaag ttcttgcctt cagtacggtt
     2341 caggccggca atgcgaccgt tgtaagcaga tgccatcacc acttcacctg aagccaagaa
     2401 ttgcaaaggt tgcgcgccag cttcccacca cacgatattg ggtttcaatt catccaactt
     2461 tttgaatgca cggtccacgc cttcaggagt gcccaaaact tggtacaact cttctttttt
     2521 cacgccatcc gccagcaaag cgaactccag cgagtatttg gcgcctttac gcaatgagcg
     2581 tttgcctggg aattttttgg tgtcccagaa atcagcccaa gattgcggtg cggttttgag
     2641 tttgtcacca tcgtaagcaa tggcggtaga ccaaacaatc gcacccacgc cacaatcgcc
     2701 ggccgcacca tcaatgaact tgtctttgcc acccaatttg ttccaatcca gtttttccaa
     2761 caagccatcg gtacaggcgc gagccaagtc ttcggattca atctgcacca cgtcccagtt
     2821 aggattgcca ccttggactt tggcttgtat cacaccataa cctccgtcgt aattttcatc
     2881 caaaacaggc ttacccaatt tttctgagaa cggtttgaaa tagatttttt tctgtgcatc
     2941 ttggtagttt ccgccccaag aggcgatggt caaatcgcgt gagcctgagc tggcagcagg
     3001 tgcggcagaa gctggcgcat tagaagctgc aggcgtgtta gaagctggtg tggtgttaga
     3061 accgccacca caggcagtga tggccaaagt tcccatgatc agagcaagac gagtgatgtt
     3121 cattttgatt accttcctaa agttgagtat atgcataatg cattcttact tgagaagtgt
     3181 ggcaacagga agtgtgtcct nnnnnnnnnn cggtacatca attaccgcag gtcacaagct
     3241 tatgctgaac ctcactccct aatcttgagg tgttgccaac ttgatgcttg taacccaaac
     3301 atgaaacaga ctgaactacc aaaaacccga tgttgtgtgt gcgtttcccc tttctatgct
     3361 tgcaaagctt gtttcaacgc agcaaccaac atttgggcat gctcttgttc aaacaccaat
     3421 ggcggtcgaa tcttcaaaac tttggcatca gggccagaat tgctgagcaa cacgccgttt
     3481 tgacgcatgt gattggtcac gcgtgtcgcc cgttcagtgg ctgtttcctt gccatctgcg
     3541 gcaatttcaa tgccccaata caagcctctg cctctgatgt ctacagtacc caaacccgtt
     3601 aattcttggg caaaggcggc ctgtatgccc aatcccagtt cttctgcttt ggcaggcaat
     3661 tgccgttctt ccaattcgtc caataccgcc atccctgtgg ccaccgccac aggattgcca
     3721 ccaaaggtat tgaagtagcg tccttttgca gcgaaagtgg ccaccaagtc atccccagcc
     3781 accacacctg ctatgggata gccgttgccc atgggtttgc ccatggtcac gatgtcaggt
     3841 tgcaaattcg catccacaaa gccccaccaa ttgtcaccgg tgcgtccaaa tccagactgc
     3901 acttcatcgg caatcacgac ggcgcccatt tttttggtgg cttttgccgc ttcattcaac
     3961 aaacccgcag acggaggtaa tatgccatcg ctggcaaaaa tcatgtccat cagtaaggcg
     4021 caaggctggt atccagcggc ctgtaaatcc tcaaacgctt tagtcacatc cacagcaaaa
     4081 cgcttggctt cagtagcatt gcccgcctca tccgcagcag cacgtggcgg tgccaccaca
     4141 cgcacaaaat cgtctatcgg agccaatgat ggcgacaatt ttgccaacaa atgactgaca
     4201 ccgtggtaag cattgctggt caccaccaca cctgtgcctc ctgtcactgc ctcggcaatg
     4261 cgtatggcca aatcattggc ttcactgcct gtacaggtaa gcattaagcg gtttaaatag
     4321 ctggggaaac gtgccaacaa gcgatcggca taagtcaaaa tcccatcgtg taaataacgg
     4381 gtatgtgtac acaaggtaga cgcttgagcc gtcatcgc
//
LOCUS       DAOMVK010000155         4365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_5754_length_4365_cov_3.226450, whole genome shotgun sequence.
ACCESSION   DAOMVK010000155 DAOMVK010000000
VERSION     DAOMVK010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5754_length_4365_cov_3.226450"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /gene="dusA"
                     /locus_tag="PK856_06210"
     CDS             <1..946
                     /gene="dusA"
                     /locus_tag="PK856_06210"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HRL01998.1"
                     /translation="DWTDRHYRHMARQITHHAWLYTEMVTTGALIHGHRGRFLDFNEC
                     EQPVALQLGGSDTADLAQCAQIAAEWGYDEVNLNCGCPSERVQKGAFGACLMNEVDLV
                     ADGLKAMQDVVDIDVTVKHRIGVDKQEEYAVVRDFVGTIAEKAACKTFIVHARNAWLQ
                     GLSPKENREIPPLKYEYVYQLKRDFPELEIILNGGVTDNAQVAEHLQYVDGVMVGRSA
                     YHQPLTMMAQWDALFYGDAHETMTFEALLPLLQSYARSHLQADKGTQLRHMARHYLGL
                     SHGMQGARQWRRMLSDATLLADNNPQLIAQAGQALNLR"
     gene            1008..1607
                     /gene="tehB"
                     /locus_tag="PK856_06215"
     CDS             1008..1607
                     /gene="tehB"
                     /locus_tag="PK856_06215"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: protein motif:HMM:NF008405.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HRL01999.1"
                     /translation="MSVSADYFQQQYGLSATHSEVVAWLEAGYLNHGHVLDLGCGRGR
                     NALYLAKHGFDVHACDADANSIANLNHIIAAEHMNNIETDVYDINRAVVQSTYDVVVS
                     TVVMQFLQADRIEAIIRNMQAQTAVGGWNLIVSAVATEGVTVPDFLKFYFQPEALREY
                     YADWDVVKYNENEGELHRTDANGNRIRLKFATLWARKRA"
     gene            1904..3928
                     /locus_tag="PK856_06220"
     CDS             1904..3928
                     /locus_tag="PK856_06220"
                     /inference="COORDINATES: protein motif:HMM:NF010038.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligand-gated channel protein"
                     /protein_id="HRL02000.1"
                     /translation="MRTRFFHSTLSVAIFAAFATPVLADTSTQSTEADTELEEIVVTA
                     SGFAQKAKEAAASITVISSKDLETKAYRDVTDALKDVPGVVVTGGGSSSDISIRGMGS
                     AYTMMLIDGKRVNSRGVRPNSDNAGIEQGWMPPITAIQRIEVIRGPASALYGSDAMGG
                     VVNIITKKTADAWMGSIKTDATIQANSEGGNTYQTGVFVSGPLIQDVLGVRGTANYSH
                     REEDKIIGGYKEQTMRNGNLVLSLTPNDQNTIEFEVLRLLQNRNATVGKTIDPTPTVS
                     KGKLTYPTDSLTDYDRTQYSLTHQGYFENFTTQTYLQREENDNPSRNMFETNTILNTQ
                     NQFKFGNHALNVGGRWQREQLDDAGNQLSINGTTLNQLERDSWAIFAEDEWRIVPQFA
                     LTGSLRYDQDENYGSNVTPKLYGVWQPNDQWIVKGGISKGYKTPALRAATDGWGQTTG
                     GGRSKGMIIGNSNLKPEKSTNVELSFNWDNNDNLAAGVTFFNTNFKDKITEARSCVGD
                     DVPGTLTCQWKGESFDFISQRENVDEAQMRGIEATFDWKITPTLDLAANYTYTDTEQK
                     SGAFKGKPLNQMPKHMFNTTLNWQAHEKLSTWGRLNYRSRTSEYLSRTSMADSTPAYT
                     FVDMGVVYKPKAEVNLTAGVYNVFDKKVDNADYGTTLDGRRFNVGFQYTF"
     gene            complement(3997..>4365)
                     /gene="trkD"
                     /locus_tag="PK856_06225"
     CDS             complement(3997..>4365)
                     /gene="trkD"
                     /locus_tag="PK856_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Responsible for the low-affinity transport of
                     potassium into the cell; involved in potassium ion uptake
                     under hyper-osmotic stress at a low pH; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HRL02001.1"
                     /translation="LHEHNILLNIVTDNQPYVEREQRLQFKRLNEQFSQMTVHYGFKQ
                     TPDLLKVMAYAQEHGYELELMRTSFFLASDSLALAPQGKLSRLRTRLFIWLARNQTQI
                     TDYCHIPHNRVVSLGGQIML"
BASE COUNT         1189 a          922 c         1121 g         1133 t
ORIGIN      
        1 ggactggact gaccggcatt accgccacat ggccagacaa atcacccacc acgcttggtt
       61 gtataccgaa atggtgacca cgggtgcgct gatacatggt caccgtgggc ggtttttgga
      121 cttcaatgaa tgtgaacaac ctgttgcttt gcaattgggt ggctcagata cggcggattt
      181 ggcgcaatgt gcacaaattg cggctgagtg gggttatgac gaagtcaatc tgaattgcgg
      241 ttgcccgagt gagcgcgtgc aaaaaggtgc atttggtgct tgtttgatga acgaggtgga
      301 tttggttgcc gatggcttga aagccatgca agacgtggtg gacattgatg tgacagtaaa
      361 acaccgcatc ggcgtggaca agcaagaaga gtatgcggtg gtgcgtgatt ttgttggcac
      421 cattgccgaa aaagcggcct gtaaaacctt catcgtgcat gcgcgcaatg cgtggctaca
      481 aggtttgtct ccgaaagaaa accgcgaaat tccaccgttg aaatacgaat acgtttacca
      541 attgaagcgc gattttccag agttggaaat catcttgaat ggcggcgtaa cggacaacgc
      601 gcaagtggcc gagcatttgc aatatgtgga tggggtgatg gtggggcgca gtgcttacca
      661 ccagccactc acgatgatgg cacagtggga tgcactgttt tatggcgatg cccatgaaac
      721 catgacgttt gaagccttgc tgccattgtt gcaaagctat gcccgaagcc atttacaggc
      781 cgataaaggc acacaattgc gccacatggc acgccattac ttgggtttga gtcatgggat
      841 gcaaggcgca cgacaatggc ggcgcatgtt gtcggatgcc accttgttgg cagacaacaa
      901 tccccaatta attgcccaag caggacaagc cttaaatctg agataaatca agcggcctgt
      961 aaaccgctgt ggcatcatgt gttttttacc tgtggagtgt gtttaagatg agcgtcagtg
     1021 ctgattattt ccaacaacaa tatggcttgt ctgctaccca ttcggaagtg gtggcttggt
     1081 tggaggcagg gtatttgaat catggtcatg tcttggattt gggctgcggt cgcggtcgca
     1141 atgccttgta tttggccaaa catggttttg atgtgcatgc ttgcgatgca gatgcaaaca
     1201 gcattgcaaa cttgaatcac atcattgccg ccgagcacat gaataacatc gaaaccgatg
     1261 tgtacgacat caatcgcgcc gtggtgcaat cgacttatga tgtggtggtg tctacggtgg
     1321 tgatgcaatt tttacaggcc gaccgcattg aggccattat ccgtaatatg caagcacaaa
     1381 cggcggtggg cggttggaac ctgatcgtca gtgccgtggc aactgagggc gtgacggttc
     1441 ctgacttttt gaagttttat tttcaacccg aggcattgcg cgaatattat gccgattggg
     1501 atgtggtcaa atacaatgaa aacgagggcg aattgcaccg taccgatgcc aatggcaatc
     1561 gcatacgcct gaagtttgcc accctgtggg cacgcaagcg cgcttaaaat acttgatagt
     1621 cagttttgcc atcggcctgt aagtttacag gccgattttt tattattggg aatcaattta
     1681 ggtggagttg taatggtgaa tatggcatga aaaatcgaaa acactgttct aaaatttggt
     1741 agttttcaat atttgccgct ctttaaaaga atatccattg aatttaagca ctgctatttt
     1801 aattaatgat tgatggtgat agtgattatc atttataatc atcacgaaat tttacagtgt
     1861 tggatttttt gtttcttgtc agttaattaa agggtagatc atcatgcgga cgcgtttttt
     1921 ccactccacc ttgtctgtgg ccatttttgc ggcatttgca acacctgttt tggcagacac
     1981 ctccacgcaa tcaaccgaag ccgatactga gctagaggag attgtggtga ctgcctcagg
     2041 atttgcacaa aaagcgaagg aagcagcggc ttcaattacc gtcatcagca gcaaagactt
     2101 ggaaaccaaa gcctatcgcg atgtgactga tgctttgaaa gacgtgcctg gtgtggtggt
     2161 aactggaggt ggaagttcta gcgatatcag cattcgcggc atgggcagtg catacaccat
     2221 gatgttgata gatggaaagc gggtcaattc gcgtggtgtg cgtcctaaca gtgacaatgc
     2281 gggcattgag caggggtgga tgccgccgat taccgccatt cagcgcattg aagtcattcg
     2341 tgggcctgct tcggctttat atggctcaga tgcgatgggt ggggtagtga acatcattac
     2401 caaaaaaacc gccgatgctt ggatgggttc gattaaaacc gatgccacca tacaagcgaa
     2461 cagtgagggt ggcaatacct atcaaacagg tgtgtttgtt tcaggcccct tgattcaaga
     2521 tgttttgggt gtgcgtggta ctgccaatta ttctcaccgt gaagaagaca agattattgg
     2581 aggttacaaa gagcaaacca tgagaaatgg taatttggtg ttgtcattaa cgccaaacga
     2641 tcaaaatacc atcgaatttg aagtgttgcg tttattgcaa aatcgcaacg cgactgtagg
     2701 caaaaccata gaccccactc caactgttag caaaggcaaa ctaacatatc caaccgattc
     2761 attaacggat tatgatcgca cccaatacag cctcacccat caaggttatt ttgaaaattt
     2821 caccacgcaa acgtatttgc aacgcgaaga aaatgacaat cctagtcgca acatgtttga
     2881 aaccaacacc attttgaata cgcaaaacca atttaaattt ggtaaccatg ctttgaatgt
     2941 gggcggtcgt tggcaacgtg aacagttaga cgatgctggc aaccagttgt ctatcaatgg
     3001 cacgaccttg aaccaattgg agcgagacag ttgggcgatt tttgctgaag atgagtggcg
     3061 cattgtgcct cagtttgcat taacaggctc tttgcgttac gaccaagatg agaactatgg
     3121 ctcaaacgtg acaccgaaat tgtatggtgt atggcagccc aatgaccaat ggattgtgaa
     3181 gggtgggatt tcaaaagggt ataaaacccc agccctgcgc gcagcaaccg atggttgggg
     3241 tcaaaccact ggtggtggca gaagcaaagg catgatcatt ggtaattcca atttgaagcc
     3301 tgaaaaatcc accaatgtgg agttgagctt taactgggac aataatgaca atttggccgc
     3361 aggggttacc ttctttaaca ccaatttcaa agataagatt accgaagcca gaagttgtgt
     3421 cggtgatgac gttccaggaa cactaacgtg tcaatggaag ggtgagtcat ttgatttcat
     3481 cagccaacgt gaaaacgttg atgaggctca aatgcgcggc attgaagcca catttgattg
     3541 gaaaatcacg cctaccttag atttggccgc taactatacc tatacggata ccgagcagaa
     3601 aagtggtgcg tttaaaggca agcctttaaa tcaaatgcct aagcacatgt ttaacaccac
     3661 tttgaattgg caagcccatg aaaaattatc gacatggggt cgcttaaatt atcgcagccg
     3721 tacctcagag tatttgagcc gaaccagcat ggcagacagt actccagcct acactttcgt
     3781 tgatatgggt gtggtttata aacccaaagc tgaggtgaat ttgactgcag gtgtttacaa
     3841 tgtgtttgat aaaaaagtag acaatgctga ttacggcact accttagatg gccgccgttt
     3901 taatgtggga tttcagtaca cattttaaat aaaccactgc atgatataaa agcggcctgt
     3961 aaaccttaca ggccgctttc tcaatgttca aacgccttac aacatgattt gcccgcctaa
     4021 agacaccaca cggttgtggg ggatatggca atagtcggtg atttgggttt ggttgcgcgc
     4081 cagccaaatg aacaaacgcg tgcgcaagcg gctgagtttg ccttgtggcg ccaaggccaa
     4141 gctgtcgctg gcaaggaaga atgaggtgcg catcaattcc aattcatagc catgctcttg
     4201 cgcgtaagcc atcaccttga gcaaatcagg cgtttgctta aagccataat gcacggtcat
     4261 ttggctgaat tgctcgttca agcgtttgaa ttgcaaacgc tgctcccgct ccacataagg
     4321 ctgattgtcg gtaacgatgt tgagcaaaat attgtgctca tgcaa
//
LOCUS       DAOMVK010000156         4324 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_5834_length_4324_cov_3.293043, whole genome shotgun sequence.
ACCESSION   DAOMVK010000156 DAOMVK010000000
VERSION     DAOMVK010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5834_length_4324_cov_3.293043"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="PK856_06230"
     CDS             <1..515
                     /locus_tag="PK856_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRL02002.1"
                     /translation="SFFVKAAVAALKKFPVVNASIDGKDVVYHGYFDIGVAIGSPRGL
                     VVPILRNADQMSLADIEKQIAEFAVKAKDGKIALEDLTGGTFSITNGGTFGSMMSTPI
                     INPPQAAILGMHATKERAMVENGQVVVLPMMYLALSYDHRLIDGREAVLTLVAIKDAL
                     EDPARLLLDI"
     gene            570..836
                     /locus_tag="PK856_06235"
     CDS             570..836
                     /locus_tag="PK856_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02003.1"
                     /translation="MKQVISLLVVGVLLAACKSGGNGGPVHYNAKAINASGQIVNSKT
                     NFTTNKEGVELTVNTLCKANRNARQVIVETRSGKQIEGSPFRCR"
     gene            888..2321
                     /gene="lpdA"
                     /locus_tag="PK856_06240"
     CDS             888..2321
                     /gene="lpdA"
                     /locus_tag="PK856_06240"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRL02004.1"
                     /translation="MSNQFDVVVIGAGPGGYIAAIRAAQLGFKTACIDAGRNKAGDAP
                     ALGGTCLNVGCIPSKALLQSSENYHQAHAEFAEHGIDVTVNNFDATKMIERKDGIVTK
                     LTGGIGFLFKKNKVESIHGLGSLKGQSGDLWQVEVDNQGEKSVVEAKHVIIATGSVPR
                     PLPGADVDNQAILDNEGALNLTKVPAKLGLIGSGVIGLEMGSVWNRLGADVTVLEAMP
                     TFLANTDQQIAKEAFKVLTKEQGMSILLGVKINKVTNNGESVTVDYEVGGEAKSDTYD
                     KLIVAIGRVPNTNGLNAEAVGLAKDERGFITVDADCRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHLDFNTIPFVVYTHPEIAWVGKTEEQLKSDGVKYAKGTSGF
                     AANGRALGLGQAQGTVKVLKDAETDRVLGVHIIGPNASELIAEAVMAMEFKASAEDIG
                     RIVHAHPTLSEVLHEAALATDKRALHG"
     gene            2372..2665
                     /locus_tag="PK856_06245"
     CDS             2372..2665
                     /locus_tag="PK856_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009426921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02005.1"
                     /translation="MNTEVLGLIALTMGVIFLCSAAVWQLNVVLTESYTLNRFSGRQL
                     FVVVTTMMITFSLAVYGFCPNARKKGMIFLLLVLLGVGLYGWAYWVLPFQAKP"
     gene            2749..3915
                     /gene="sucC"
                     /locus_tag="PK856_06250"
     CDS             2749..3915
                     /gene="sucC"
                     /locus_tag="PK856_06250"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HRL02006.1"
                     /translation="MNLHEYQAKALLASYGLPVQGGILATNADEAAAAFDELGGKFAV
                     VKAQVHAGGRGKAGGVKVVKTREEAKEVATSLIGTNLVTYQTDANGQPVNSVLICEDM
                     YPVARELYLGAVVDRSSRRITFMASTEGGVEIEKVAEETPEKIIKVTVDPLVGLLPFQ
                     AREVAFALGLEDKQVNQFVKLMTGAYQAFVENDFALFEINPLSVRENGELACVDAKIG
                     IDSNALYRLPEIAAQRDKSQENERELKASEFDLNYVALEGNIGCMVNGAGLAMATMDI
                     IKLYGGQPANFLDVGGGATKERVVEAFKLILADPSVQGVLVNIFGGIVRCDMIAEAII
                     AAVKEVHVTVPVVVRLEGNNAELGAQILRESGLQLIPAEGLADAAQKIVDAVKA"
     gene            3927..>4324
                     /locus_tag="PK856_06255"
     CDS             3927..>4324
                     /locus_tag="PK856_06255"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Catalyzes the only substrate-level phosphorylation
                     in the TCA cycle; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HRL02007.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQKHLDLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLIVVITEGVPT
                     IDMLKAKRYLETNGNGTRMVGPNCPGVITPG"
BASE COUNT         1143 a          813 c         1162 g         1206 t
ORIGIN      
        1 tgtctttctt tgttaaagct gcggttgctg cattgaaaaa attcccagtg gtgaatgcat
       61 ctatcgatgg caaagatgtg gtttaccacg gttactttga cattggtgtg gccattggca
      121 gtccacgcgg tttggtagtg cctattttgc gcaatgctga ccaaatgtca ttggctgata
      181 ttgaaaaaca aattgcagag tttgcagtaa aagccaaaga cggtaaaatc gctttagaag
      241 acttaactgg tggtactttc agtattacca atggtggtac ttttggttct atgatgtcta
      301 ctcctatcat caacccgcct caagcggcga ttttgggtat gcatgcaacc aaagaacgtg
      361 ccatggttga aaatggccaa gtagtggtat tgccgatgat gtacttggct ttgtcttacg
      421 atcaccgctt gattgatggt cgtgaagctg tattgacgtt ggtagcaatt aaagacgcat
      481 tggaagaccc agctcgtttg ttgttggaca tctaacctag gaatgcggcg actgaggttg
      541 ccgcaggact gcttttgaaa ggaatgaaca tgaaacaggt aatttcattg ttggttgtgg
      601 gtgtcttgtt agcggcctgt aaatcaggtg gcaatggcgg tccagttcac tataatgcca
      661 aagcaatcaa tgcttctggt caaatcgtga acagtaaaac caatttcacc accaataaag
      721 aaggtgtgga gttgacggtc aataccttgt gtaaggccaa tcgcaacgct cgacaagtca
      781 tcgtagaaac tcgttccggt aagcaaattg aaggcagtcc tttccgctgt cgttaatacc
      841 atttaaaaca caggtcgctt tttatgcggc ctgtaaaaag gatttttatg tctaatcagt
      901 ttgatgtagt agtcataggc gcaggccctg gcggctatat tgcggccatt cgtgctgcgc
      961 aattgggttt taaaaccgct tgtattgatg caggccgcaa caaagcaggt gatgcaccag
     1021 ctcttggtgg tacctgtttg aatgtgggtt gtattccttc taaagccttg ttgcaatcat
     1081 ctgagaatta tcaccaagcc cacgctgaat ttgctgagca tggtattgac gtcactgtaa
     1141 acaactttga cgccaccaaa atgattgagc gcaaagacgg tattgtgacc aaattgacgg
     1201 gtggtattgg cttcttgttc aaaaagaaca aagtggaatc catccatggc ttgggttctt
     1261 taaaaggtca aagcggtgat ttgtggcaag ttgaggtgga taaccaaggt gaaaaatctg
     1321 tggttgaagc caaacatgtc atcatcgcta caggttctgt accacgccca ttaccaggtg
     1381 ctgatgtgga caatcaagcc attttggaca atgaaggcgc attgaatttg accaaagttc
     1441 ctgccaaatt gggtttgatt ggttctggcg tgattggttt ggaaatgggc tctgtttgga
     1501 atcgcttggg tgcagacgtg accgtgttgg aagcgatgcc aacattcttg gccaataccg
     1561 atcagcaaat tgccaaagaa gcgttcaaag ttttgaccaa agaacaaggc atgagcatct
     1621 tgttgggcgt gaaaatcaac aaagtcacca ataacggcga atctgttact gtggattacg
     1681 aagttggtgg cgaagccaaa tcggatactt acgataaatt gattgtggcg attggtcgcg
     1741 tgccgaacac caatggattg aatgcggaag cagttggctt agctaaggat gagcgtggct
     1801 tcatcactgt tgatgcagat tgtcgcacga acttgcctaa tgtttgggca atcggtgacg
     1861 tggtacgtgg ccctatgttg gcgcacaaag ccagtgatga aggtgtggcc gtagctgagc
     1921 gcatagctgg ccaaaaaccg catttggatt tcaataccat tccatttgtg gtttacaccc
     1981 acccagaaat tgcatgggta ggcaagactg aagagcagtt gaaatcggat ggcgtgaaat
     2041 atgccaaagg tacttctggt tttgctgcca atggtcgtgc attgggtttg ggtcaagcac
     2101 aaggtacggt taaagtattg aaagatgctg aaactgatcg tgtgttgggt gtacacatca
     2161 tcggaccaaa tgcttcagaa ttgattgctg aagcggtgat ggccatggag tttaaggctt
     2221 cagctgaaga catcggccgc attgttcatg cacacccaac tttgtctgaa gtattgcacg
     2281 aagcggcatt ggcaaccgat aagcgtgcat tgcacggtta atagaagcgc agcttaggct
     2341 gcgtttttat ttacatctaa ataaaaaaca gatgaataca gaagtactgg gtttaattgc
     2401 tttgacaatg ggcgtgatat tcttgtgctc agcagcggta tggcaattaa atgtggtgtt
     2461 aacagaaagt tacaccttaa atcgattttc aggacgccaa ttgtttgtgg tggtcaccac
     2521 catgatgatt acattcagtt tggcagtata tggcttttgt cccaatgcgc gtaaaaaagg
     2581 catgattttc ttgttgcttg tgttgttggg agtggggtta tatggttggg cgtattgggt
     2641 attgccattt caggcgaaac cataagcacg ggtctgaatg gcgaagtgat gtgtattaac
     2701 ataatgattg cgtgagcaat ccgtttaact tacctagagg aaactctgat gaatttacat
     2761 gaatatcaag caaaagcctt gttagcaagc tatggcttgc cagtacaagg tggtatcttg
     2821 gccaccaacg ctgacgaagc tgcggctgcg tttgacgaat taggtggtaa gtttgcggtg
     2881 gtgaaagccc aagtgcatgc tggcggtcgt ggtaaggctg gtggcgtgaa agtggttaaa
     2941 acccgcgaag aggccaagga agttgccaca agcttgattg gtaccaattt ggtgacctac
     3001 caaaccgatg ctaacggtca gcctgttaac agcgtattaa tctgtgaaga tatgtaccca
     3061 gtagcccgcg agttgtactt gggtgcagtg gtagaccgtt ctagccgtcg catcactttc
     3121 atggcatcga ctgaaggtgg agtggaaatt gaaaaagtgg cagaagaaac gcctgaaaaa
     3181 atcatcaaag tgactgtgga tccgttggtg ggattgttgc ctttccaagc gcgtgaagtg
     3241 gcttttgctt tgggtttgga agacaaacaa gtcaatcaat ttgtgaaatt gatgacaggt
     3301 gcttaccaag catttgttga aaacgatttt gcattgtttg aaatcaaccc attgtctgtg
     3361 cgtgaaaacg gggagttggc ttgtgtggat gcgaaaatcg gcatcgattc aaacgcattg
     3421 taccgcttgc ctgaaattgc tgctcaacgt gacaaatctc aagaaaacga gcgtgaatta
     3481 aaagcatctg aatttgattt gaactatgtg gccttggaag gcaacattgg ctgtatggtt
     3541 aatggtgctg gtttggccat ggcgaccatg gacatcatca aattgtacgg tggtcaacct
     3601 gcaaacttct tggatgtggg cggtggtgct accaaagaac gcgtggttga agcattcaaa
     3661 ttgattttgg ctgacccatc tgtgcaaggc gttttggtta acatctttgg tggtatcgtg
     3721 cgttgcgaca tgattgcaga agcgattatt gcggcagtga aagaagtaca cgttactgtg
     3781 cctgtggtgg tgcgtttgga aggcaataat gccgaattgg gtgcgcaaat cttgcgtgaa
     3841 tctggtttgc aattgatccc agctgaaggt ttggctgatg cggctcaaaa aattgttgat
     3901 gccgttaaag cataaggagt gaatgaatga gcgtattgat taataaagat accaaagtat
     3961 tggtgcaagg ttttacgggt aaaaacggta cttttcactc tgagcaagcc ttggcttatg
     4021 gcaccaaagt tgtcggcggt gtgactccag gcaaaggcgg tcaaaagcat ttggatttgc
     4081 ctgtgttcaa taccatgaaa gaagcggtta aagaaacagg tgcggatgct tctgtgattt
     4141 atgtacctgc tcctttcgtg ttggactcca tcgttgaagc ggttgattct ggcgtgggct
     4201 tgattgtggt gattaccgaa ggtgtgccaa cgattgacat gttgaaagcc aaacgctatt
     4261 tggaaaccaa tggcaatggc acacgcatgg tcggccctaa ctgccctggc gtgattaccc
     4321 cagg
//
LOCUS       DAOMVK010000157         4321 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_5838_length_4321_cov_3.986170, whole genome shotgun sequence.
ACCESSION   DAOMVK010000157 DAOMVK010000000
VERSION     DAOMVK010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5838_length_4321_cov_3.986170"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /gene="groEL"
                     /locus_tag="PK856_06260"
     CDS             complement(<1..893)
                     /gene="groEL"
                     /locus_tag="PK856_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRL02008.1"
                     /translation="MAAKEVKFGNDARQKMVEGVNILANAVKVTLGPKGRNVVLDRAF
                     GGPHITKDGVSVAKEIELKDKFENMGAQLVKEVASKTNDIAGDGTTTATVLAQAVVAE
                     GMKYVTAGMNPMDLKRGIDKAVAALVGELKNISKAVPEKSKEIAQVASISANSDTSIG
                     DIIAQAMNEVGKEGVITVEDGKSLENEVEVVKGMQFDRGYLSPYFINHPEKQIAELDS
                     PFVLLYDKKISNIRELLPVLEQVAKTSRPLLIIAEDVDGEALATLVVNNIRGILKTVA
                     VKAPGFGDRRKAMLQDIAILTG"
     gene            complement(919..1353)
                     /locus_tag="PK856_06265"
     CDS             complement(919..1353)
                     /locus_tag="PK856_06265"
                     /inference="COORDINATES: protein motif:HMM:NF020550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HRL02009.1"
                     /translation="MAKRTTAAKTKKEPVQAAESVAVDTASVHTESDKLHSNEQQATS
                     VAGDAQNTFQFRFWELGALKGKVQGVEVGHDGAPVDLFVFDEVNFEAFKNGQQHQYIG
                     GFGITAARHLFQIPFDGTWYLAVRPVNADDHYEVAYRLMNFN"
     gene            complement(1359..1646)
                     /gene="groES"
                     /locus_tag="PK856_06270"
     CDS             complement(1359..1646)
                     /gene="groES"
                     /locus_tag="PK856_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRL02010.1"
                     /translation="MAIRPLHDRIVVKRVEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKRGDNGERQALDVKVGDKVIFGKYSGQTVKVDGQELLVMKEDEVFAIMD"
     gene            1930..>4321
                     /gene="ppc"
                     /locus_tag="PK856_06275"
     CDS             1930..>4321
                     /gene="ppc"
                     /locus_tag="PK856_06275"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HRL02011.1"
                     /translation="MLLHSLLSNPKDAPLLADVSYLSGALLDVLQHETNANVIAVLNE
                     LLKTQDATATLHKWLPQLDGNQTEQLIRACSLFAQVFNIAEDAHHERRRLSHEQSGNN
                     ERQGSLRATVSALKPHLNTAQLQAALNQTQISAVLTAHPTEVQRQTILGINRRIRTIL
                     DQRGHTLSRQDSEDLDWDIRATLLTLWQTNETRPFKISVEDEISNGLGYFNLSFLQAL
                     PKLYRNMQRTLQETYPDIELPNILQIGGWIGGDRDGNPNVSADTLRFAVRKQADVIFH
                     YYRDELAGLYKELPLSERRIDVSPAVQALSDRSPDTERARAEEPYRRAIATIVARIVA
                     SGRALGLTLGSRVGLLEPYENVEAFIADLQALSDSLQQHGSALLSHGRLQQLIRSASV
                     FGFYLMPLDLRQHAAFHADTVADLFAHAGLEDFLSLNDAERSQVLLRELSNQRPLFNR
                     FVTYSDMTQHELAIFQAAHEIKNQFGETAINQAIISNCETVSDMLCLALLLKESGLLS
                     VDDAGKPSSRINIVPLFETIEALEDCIGVMDTLFSIDWYQALLASREHRQEIMLGYSD
                     SNKDGGYVTSQWSLYQAEAQLVEVYAKHNVKMRLFHGRGGSVGRGGGPSFQAILAQPA
                     GSVASQIRITEQGEVISAKYTDVANAERNLETLVAATLEASLLPHATEEPDSGLMQAL
                     SDNAFAHYRKLITHQGFIDFFLQTTPIEAIASLNIGSRPASRKTLAKIQDLRAIPWVF
                     SWTQTRLILPAWYGFGTAVEAVCADNEHALQSLHHQAKHSAFIQAMLSNMEQVLAKT"
BASE COUNT         1069 a         1159 c          969 g         1124 t
ORIGIN      
        1 ccagtcaaaa tcgcgatgtc ttgcaacatg gctttgcgac ggtcgccgaa accaggggct
       61 ttcacagcca cggttttcaa aatgccacgg atgttgttca caaccaaagt cgccaaggct
      121 tcgccatcca cgtcttcagc aatgatcaac aatgggcggc tggttttggc cacttgttcc
      181 aaaacgggca acaattcgcg gatgttgctg atttttttgt cgtaaagcaa tacaaatggg
      241 ctgtctaatt cagcgatttg tttttcaggg tggttgatga agtaaggaga caagtaaccg
      301 cggtcaaact gcatgccttt aaccacttct acttcgtttt ccaatgattt gccgtcttct
      361 acggtaatca caccttcttt acccacttca ttcatcgctt gagcaatgat gtcgccgata
      421 gaagtgtcag agttggcaga aatagacgcc acttgcgcga tttcttttga tttttctggt
      481 acggctttag aaatgttttt caattcgccc accaaagccg caacggcttt atcgatgccg
      541 cgtttcaaat ccataggatt catgcccgca gtcacgtatt tcatgccttc agccacaaca
      601 gcttgagcca atacggttgc agtcgtggta ccgtcacctg cgatgtcgtt ggttttagaa
      661 gcgacttctt tcaccaattg cgcacccatg ttttcgaatt tgtctttcaa ttcgatttct
      721 ttggccacag acacaccgtc tttggtgatg tgtgggccac cgaatgcacg gtccaatacc
      781 acgttacggc ctttagggcc caaggttact tttacggcgt tggccaagat gtttacacct
      841 tcaaccattt tttgacgggc atcgttgcca aatttaactt cttttgcagc catgttacat
      901 ctccaaaatt aactagattt aattgaaatt cattaagcgg taagccactt cataatggtc
      961 atctgcattg acaggacgca cagccaaata ccatgtgccg tcaaacggaa tttggaacaa
     1021 atggcgggcg gcggtgatgc caaacccacc aatgtattgg tgttgctgac cattcttaaa
     1081 ggcttcaaaa ttgacttcat caaaaacgaa caagtctact ggtgcaccat cgtgccccac
     1141 ttcaacacct tgaactttgc ctttgagcgc acccagctcc caaaaacgaa actgaaaggt
     1201 gttttgagca tcgccagcaa ccgatgtggc ttgttgttcg ttactgtgta acttgtctga
     1261 ttctgtgtgc acactcgccg tgtctaccgc cactgattca gcggcttgaa cgggttcttt
     1321 tttggttttt gcagccgttg ttcgttttgc cataatgatt aatccatgat tgcgaaaact
     1381 tcgtcttctt tcattaccaa caattcttga ccgtctactt taacggtttg gcctgagtat
     1441 ttgccgaaaa tcactttatc gcccactttg acatccaaag cctgacgttc gccattgtcg
     1501 ccacgtttgc cagcacccac agcaatcact tcacccatat caggtttttc agccgctgca
     1561 cctggcaaca ctatgcctga agccgttttt tcttctgctt caacacgttt aactacgatg
     1621 cgatcatgta aaggacgaat agccatgttt tcattctcca aaaaaaaacg taagcggact
     1681 gtgccgcgtt ccatcaaata aatgcgcctg tcattcaaac acacgctaac aatcttactg
     1741 ggtgaacaaa taaggctgat acagcaaaat tcaagcccca agatttaaaa aaaattacat
     1801 tgtatttcaa tcatctgttt ttacaggccg cttaatttcg gcctgaattc ttgctcaact
     1861 ctatgaaagg cgtggctttt ctctgggaag gggtttaaca tcgttaacct gttcttgaag
     1921 gaaagccgta tgctgttaca ctcactgctc agtaatccca aagatgcacc cttacttgcc
     1981 gacgtctctt atttaagtgg cgctttgttg gacgtgttac aacacgaaac caatgccaac
     2041 gtgattgcgg ttctgaacga actgctcaaa acccaagatg ccacggcaac gctacacaaa
     2101 tggctgccgc aattggatgg caatcaaacc gaacaattga tccgcgcatg cagcttgttt
     2161 gcacaagtat tcaacatcgc cgaagacgca caccacgaac gccgccgttt gagccacgag
     2221 caatcgggca ataacgagcg tcaaggcagc ttgcgtgcca ccgtcagcgc tttgaagccg
     2281 catttaaata cagcgcaatt acaggccgca ttgaatcaaa cccaaatttc agcggtattg
     2341 actgcccacc caaccgaggt gcagcgtcaa accattttgg gcatcaaccg ccgcatccgc
     2401 accatcttag accaacgcgg ccacacctta agccgccaag acagtgaaga tttggattgg
     2461 gacatacgtg ccaccttgct gactttgtgg caaaccaacg aaacccgccc cttcaaaatc
     2521 agcgtggaag atgaaatcag caatggcttg ggctatttca atttaagctt cttgcaagcc
     2581 ttgcccaagc tgtatcgcaa catgcaacgc accttacaag aaacctatcc cgacatcgaa
     2641 ttgccgaata ttttgcaaat cggcggctgg ataggtggcg accgcgatgg caatccgaat
     2701 gtgtctgccg acaccttgcg ctttgccgtg cgcaaacagg ccgatgtgat tttccattat
     2761 taccgcgatg aattggcggg tctctacaaa gaattgcctc tgtctgagcg ccgaatcgat
     2821 gtatcgcctg cggtgcaagc cttgtctgac cgctcgcccg acaccgaacg cgctcgcgcc
     2881 gaagagccat atcgccgcgc catcgctacc attgttgccc gtatcgttgc ctcaggccgc
     2941 gccttgggtt tgactttggg ttcacgcgtg ggtttgcttg agccatatga aaacgtcgaa
     3001 gcctttattg ccgacttaca agccttgtct gattctttgc aacaacacgg cagcgccttg
     3061 ttaagccacg gccgtttgca acaattgata cgctcggctt cagtgtttgg tttctacttg
     3121 atgcctttgg atttgcgcca acacgcggct ttccatgccg acaccgtggc cgatttgttt
     3181 gctcacgcag gtttggaaga cttcttaagc ctgaacgatg ccgagcgcag ccaagtgttg
     3241 ttgcgcgaac tgtcgaatca acgcccgctg ttcaaccgtt ttgtcactta cagcgacatg
     3301 acccaacacg aattggcgat ttttcaggcc gctcacgaga ttaaaaacca attcggcgaa
     3361 accgccatca accaagccat catctccaat tgcgaaaccg tcagcgacat gctgtgcttg
     3421 gctttgctgt tgaaagaatc tggcctgttg tcggtagacg atgcaggaaa accgagctca
     3481 cgcatcaaca ttgtgccttt gtttgaaacc atcgaagcct tagaagactg tataggtgtg
     3541 atggacacct tgttcagcat agattggtac caagccttac tcgccagccg cgaacaccgc
     3601 caagaaatca tgctcggtta ttccgacagc aacaaagacg gtggctatgt caccagccag
     3661 tggtcgctct accaagccga ggcacaattg gttgaggtat acgccaagca caatgtcaaa
     3721 atgcgcctgt tccacggccg tggcggcagc gtgggccgtg gtggtggccc ttctttccaa
     3781 gcgattttgg cacagcctgc aggttcggtt gccagccaaa ttcgcatcac cgagcaaggt
     3841 gaggtaattt cggccaaata caccgatgtg gccaatgccg aacgcaattt ggaaaccttg
     3901 gtggctgcca ctttggaagc cagtctgttg ccgcacgcaa ccgaagaacc agactctggc
     3961 ttgatgcaag ccttgtctga caacgcgttt gcccattacc gcaagctgat tacccaccaa
     4021 ggcttcattg atttcttctt gcaaaccacg ccaattgaag cgattgccag cctcaatatc
     4081 ggtagccgcc ctgcttcgcg caaaactctg gcgaaaatcc aagatttgcg tgcgattccg
     4141 tgggtattct cttggacaca aacgcgcttg attttgcccg cttggtacgg ctttggtacg
     4201 gcagtggaag ccgtgtgtgc cgacaatgag cacgctttgc aaagcctgca tcaccaagcc
     4261 aaacattcag ccttcatcca agcgatgttg tcgaacatgg aacaggtatt ggcgaaaacc
     4321 g
//
LOCUS       DAOMVK010000158         4310 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_5858_length_4310_cov_3.068860, whole genome shotgun sequence.
ACCESSION   DAOMVK010000158 DAOMVK010000000
VERSION     DAOMVK010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5858_length_4310_cov_3.068860"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="PK856_06280"
     CDS             <1..815
                     /locus_tag="PK856_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein adenylyltransferase SelO family protein"
                     /protein_id="HRL02012.1"
                     /translation="PYLAMFATISQRSAELVAAWQSVGFCHGVLNTDNMSALGLTIDY
                     GPFGFLDAFDPHHICNHSDTQGRYAYHAQPAIVQWNLSCLASCFLPLATEDELVSVLN
                     DYVAQYKQAYLERMRAKLGLMRPVNEDQALIDGLLKAMAAQRVDYSLCLRHLSLVSRN
                     GDDIPAELAALWPLPDTGLQQWLTLYRERLQVESLSDAERATAMNAVNPKYVLRQYLL
                     QDAIAKAEQGDASGITELAFVMSHPYDEQVEYQSYAALPPDWAAGICVSCSS"
     gene            832..2082
                     /locus_tag="PK856_06285"
     CDS             832..2082
                     /locus_tag="PK856_06285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRL02013.1"
                     /translation="MSTICLNMIVKNEASIIVDTLNNILAHIRIDYWIIADTGSDDGT
                     QDIIQAFFQQRGIPGELLQHAWKNFGYNRELALQAAQGKSDYVFFFDADDRFEGTFVL
                     PESLTATIYNFYLTNMARSTRYPRRLLVKNDGSCQWIGVLHEVITAKNAATSNEAYID
                     GNYHVISGRFGARNQNPNKYLDDAHMLEEAFAQENNVALKRRYAYYCGQSYRDCNQPQ
                     MATVWYERNIELCNKTGEEIRFSYIALGTEYRKLNDSAKTLEAWWNAYNAMPQNAEAL
                     GLIAEYLYVLERYHLGLEVAKKAATLPDPVPHATLFVNEPVHRYVIWYELGRHAVKLS
                     MWDDAYASAKHLLNQIEHSDALNNFILSILEALKGHIERDTFANARQIQQRLAAMQQL
                     SSDDTRHKHHEMLNWFAQAFNNPA"
     gene            complement(2160..2858)
                     /locus_tag="PK856_06290"
     CDS             complement(2160..2858)
                     /locus_tag="PK856_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HRL02014.1"
                     /translation="MPSQKAMATQLKLANQAGRVHLLTPDSVIRAEKLHPSANLSYKV
                     KGTRYQPIQKVSNFTQEGKASWYGKQFHGRKTASGERYDMNEMTAAHRTLPIPSYAKV
                     TNLANGKEVIVRINDRGPFHSNRVMDVSFGAAKKLGMVNSGTARVRVEQLVPGQLAQA
                     ETAQPMFVSLQKFDQVQDAQAYLQRVNALVSKEQHGKKVSMVREGDKYVVQVGPFQSH
                     EKTEPFKQTMLTAL"
     gene            2857..>3036
                     /locus_tag="PK856_06295"
     CDS             2857..>3036
                     /locus_tag="PK856_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02015.1"
                     /translation="MAIVFLLVLAVSATAWLMSVLLIATAATVQKKTFFLDKLSYFLE
                     WISVYIKAGLSIYPIV"
     assembly_gap    3037..3046
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3257..>3541)
                     /locus_tag="PK856_06300"
     CDS             complement(3257..>3541)
                     /locus_tag="PK856_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007341871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRL02016.1"
                     /translation="ELADMLPPTCVELNSAAILREHKPPQSTLKHAQRMKNIRGVFTL
                     QADVNKRNLLLIDDVVTTSATLNELVRTLKTAGAATVSCWVLARAGVQKI"
     assembly_gap    3542..3592
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3593..4031)
                     /locus_tag="PK856_06305"
     CDS             complement(<3593..4031)
                     /locus_tag="PK856_06305"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HRL02017.1"
                     /translation="MLYSLLKSGAANLQAPQRCVLCHDLAKQLYLCADCALLMARLQI
                     GNEACPLCAKPSLQAAVCGECQQHPPHFERLWASYHLDWPLHSVIAEFKYQQQWSHAR
                     ALAAWMAESPPPWLHQAALDGVLAMPLSRVRLIERGYNQAHVLV"
     gene            4030..>4310
                     /locus_tag="PK856_06310"
     CDS             4030..>4310
                     /locus_tag="PK856_06310"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRL02018.1"
                     /translation="MSLTSPIVLIHGWAANHHVYDDFRHYLPDVPTLVPDLPGHGADV
                     RTQIDAAAFADELAAQIEVPANIVAWSLGGQVALQLAVRHPDKVASLCL"
BASE COUNT         1047 a         1095 c         1018 g         1089 t
ORIGIN      
        1 atccttactt ggccatgttt gccaccatca gccaacgcag cgccgaattg gtggcagcat
       61 ggcaaagcgt gggcttttgt catggtgtgc tcaataccga caatatgtcg gccttgggtt
      121 tgaccataga ctacggccca tttggtttcc tcgatgcctt cgaccctcat cacatttgca
      181 accattccga cacccaaggc cgctatgcct accatgccca gcccgccatc gtgcagtgga
      241 atttgtcgtg tttggcatcg tgcttcttgc ctttggccac agaagacgaa ttggtgagcg
      301 tgttaaacga ttatgtggcg caatacaaac aagcctattt agagcgcatg cgcgccaaac
      361 tcggcctcat gcggcctgta aacgaagacc aagccttgat tgatggcttg ttgaaagcca
      421 tggccgcgca acgggtggac tacagcctgt gcctgcgcca tttgagccta gtcagccgca
      481 atggcgatga catccctgcc gagttggccg cgctgtggcc tttgcctgat actggtttac
      541 agcaatggct cacgctttac cgcgaacgct tgcaagtaga aagcttgagc gatgccgaac
      601 gtgcgacagc catgaacgca gtcaatccca aatacgtatt gcgccaatat ttgttacaag
      661 acgccattgc caaagccgag caaggcgatg ccagcggcat caccgaattg gcatttgtca
      721 tgtcgcatcc atacgacgaa caagtcgaat atcaaagcta tgcggctttg ccacccgatt
      781 gggcagcggg aatttgtgta tcatgttcga gttaattgct taaggccgct tatgagtacc
      841 atttgcctca atatgatcgt caaaaatgaa gcttccatca ttgtggacac gctgaacaat
      901 attttggccc atattcgcat tgactattgg atcatcgccg atacaggctc tgacgatggt
      961 actcaagaca tcattcaagc cttttttcaa cagcgcggca ttccaggcga attgttgcaa
     1021 catgcgtgga aaaactttgg ctacaaccgc gaattggcct tacaggccgc acaaggcaaa
     1081 agcgattacg tgtttttctt cgatgccgat gaccgctttg aaggcacgtt tgtcttgccc
     1141 gaatcgctta ccgccaccat ttacaatttc tacctcacca atatggcgcg ctctacccgt
     1201 tatccacgcc gcttgctggt gaaaaacgat ggctcgtgcc aatggatagg cgtattgcac
     1261 gaagtcatca ccgccaaaaa cgctgccacc agcaatgaag cctacatcga cggcaattac
     1321 catgtgattt caggccgctt tggtgcgcgc aatcaaaatc ccaacaagta tttagacgat
     1381 gcccacatgt tggaggaggc atttgcgcaa gaaaacaatg tggcccttaa gcgccgctac
     1441 gcgtattact gtgggcagtc ttaccgcgat tgtaaccaac cccaaatggc cacggtttgg
     1501 tatgaacgca atattgagtt gtgcaacaaa acgggtgaag aaattcgctt cagctacatt
     1561 gcattgggca cagaataccg caagcttaac gacagcgcca aaacgctgga agcttggtgg
     1621 aacgcctaca atgccatgcc acaaaatgcc gaagccttgg gcttgattgc cgaatatttg
     1681 tatgtgttag agcgctacca tttgggcttg gaagtggcca aaaaagccgc caccttgccc
     1741 gacccagtgc ctcacgccac cttgtttgta aatgagcctg tgcaccgtta tgtgatttgg
     1801 tatgaattgg gtcgtcatgc ggtgaaattg tccatgtggg acgatgctta cgcttcagcc
     1861 aaacatttgc tcaatcaaat cgagcacagc gatgccttga acaacttcat cctcagcatc
     1921 ttggaagcct tgaaaggcca cattgagcgc gacacctttg ccaatgccag acaaatccaa
     1981 caacgcctcg ctgccatgca acaattgagc agcgacgaca cccgtcacaa acaccacgag
     2041 atgttgaact ggtttgctca ggcatttaac aacccagcct aagtgacata ctgtacaaac
     2101 aaacaaaagc ggcctgtaca catttacagg ccgctttcac atgctcacgc tgctgttgat
     2161 tacagtgcgg tcaacatggt ttgtttgaat ggttcggttt tttcatggct ttggaatggg
     2221 cccacttgaa ccacatactt atcgccttca cgcaccatgc tcactttttt accgtgttgc
     2281 tctttgctga ccaaagcatt cactcgttgc aaatacgctt gtgcgtcttg tacttggtca
     2341 aatttttgca agctcacgaa catcggttga gcagtttctg cttgtgccaa ttgaccaggc
     2401 accaattgtt caacgcgcac gcgcgccgta cctgagttga ccatgcccaa ttttttcgct
     2461 gcgccaaaag acacatccat cacgcggttg ctgtgaaatg gaccacggtc attgatgcgc
     2521 acaatcactt ctttgccatt ggcaagattg gtgactttgg cataactcgg aatcggtaaa
     2581 gtgcggtgag cggcagtcat ttcattcatg tcgtagcgtt cgccagaagc ggttttacga
     2641 ccatggaatt gtttgccata ccaagaggct ttgccttctt gggtgaaatt ggaaactttt
     2701 tgtatcggtt ggtaacgggt acctttaacc ttatagctca ggttggcgct cggatgaagt
     2761 ttttcagcac gtatcaccga gtcgggagtc agcaaatgta cacgtccagc ttggttagca
     2821 agtttcaatt gggtggccat ggctttttgg ctgggcatgg caattgtatt tttgctggtt
     2881 ttggcggtat cggccacggc ttggctgatg agtgtgctgc tgattgcaac ggcggcaact
     2941 gtgcagaaaa aaacattctt cttagacaaa ctgagctact ttcttgagtg gataagtgtg
     3001 tacatcaagg caggattgag catttatccc attgtcnnnn nnnnnnataa caatcagctt
     3061 gatggtgtgt gctttaaaac ctgtttgcac aagcaaaaaa tttgctgctt ctcagcaaca
     3121 aataacgcgg ttttttaccc aaattccctc tgtttcaaag tgactcaatc tgtgtaaatt
     3181 agagtcattt tttactgcaa aacagttaaa tcaatcactt aggcaaacgt cactatgcaa
     3241 tacatcactt ttcaggtcaa attttttgca ctcccgcccg tgccaacacc cagcaagaca
     3301 cagtcgcagc ccccgccgtt tttagcgttc gcaccaattc attcaatgtt gcagatgtgg
     3361 tcacaacatc atcgattaac agcaggttac gcttatttac atcagcctgt aaggtgaaaa
     3421 caccacgaat atttttcatc cgctgtgcgt gtttgagtgt tgattggggt ggtttgtgtt
     3481 cacgtaatat ggcagcggaa ttgagttcga cacaagtggg cggcaacatg tctgccaatt
     3541 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcaccaga
     3601 acgtgtgctt ggttgtagcc ccgctcaatt aaacgcactc ggcttaaagg catggcaagc
     3661 acgccatcca acgcggcctg atgcaaccat ggcggcggcg actctgccat ccaagccgcc
     3721 aaagcccgcg catgcgacca ttgctgttgg tatttgaatt cagcaatgac actgtgcagc
     3781 ggccaatcca aatggtaact ggcccacaag cgttcaaaat gaggaggatg ttgttgacac
     3841 tcgccgcaca cagcggcctg taagctgggt ttggcacaca aagggcaggc ttcgttgccg
     3901 atttgtaagc gtgccatcag caaggcgcaa tcggcacaca aatacaactg cttagccaaa
     3961 tcatggcata atacgcagcg ttgtggcgct tgcaagttgg cggcgccaga tttaagtaaa
     4021 gaatacaaca tgagcctcac ctcccccatc gtattgatac acggctgggc ggccaatcac
     4081 catgtgtacg atgattttcg ccactacctg cccgatgttc ccaccctcgt tcctgatttg
     4141 cctggtcacg gcgccgatgt gcgcacgcag attgatgccg ccgcatttgc cgatgaattg
     4201 gccgcgcaaa tcgaagtgcc tgccaacatt gtggcatggt ctttaggcgg acaagtggcg
     4261 ctgcaactgg cggtgcgcca tcctgataaa gtggcttctt tgtgtttgtg
//
LOCUS       DAOMVK010000159         4308 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_5863_length_4308_cov_3.974841, whole genome shotgun sequence.
ACCESSION   DAOMVK010000159 DAOMVK010000000
VERSION     DAOMVK010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5863_length_4308_cov_3.974841"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="PK856_06315"
     CDS             <1..633
                     /locus_tag="PK856_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HRL02019.1"
                     /translation="TESYEWTEGEWTLEHGFKRPVNQPYHVVAYDFGVKTNILRMLAE
                     RGCRLTVVPAQTPAKDVLALNPDGVFLSNGPGDPEPCDYAISAIQDILASKKPVFGIC
                     LGHQLLGLAVGGKTRKMPFGHHGANHPVQDVDANKVMITSQNHGFEVDEASLPANVRV
                     THRSLFDGSVQGIELTDQEAFSFQGHPEASPGPHDVAYLFDRFINAMSAK"
     gene            657..1553
                     /locus_tag="PK856_06320"
     CDS             657..1553
                     /locus_tag="PK856_06320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HRL02020.1"
                     /translation="MNAMSLPTELHDYGYVLVNVFAPSHFAGNPLAVFPHADGLSDTD
                     MQAIAIQMNLAETVFAFKSEDAVAKLRIFTPDYELPFAGHPVIGAARVLDQLKRLPAE
                     FTVQTEAGITQLKRDKMRYTFTRTQGECDEVASSEVPALLMALGLDDLSVSSAPCWVN
                     TGSEQLLLEVSQTEVLSHIKPDAEQLSAWFAKQGKRAAVYLWHEKNRYVRVRFFSAVG
                     NVLTEDIGTGSACANLGGWCLAHGLNDVNWLIQQGDYLRRPNRLHLQTSAEGLIYVGG
                     ETIVVGHGSLSVPHSVKEHEPC"
     gene            1547..2191
                     /gene="leuE"
                     /locus_tag="PK856_06325"
     CDS             1547..2191
                     /gene="leuE"
                     /locus_tag="PK856_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HRL02021.1"
                     /translation="MLGVIDLPTYVIGVIAIILLPGPNSMFCLTVGAQYGVKKGYIAA
                     AGVFIGDLVLMTLSALGAASLMIKYPAIFNIIKYVGGGYLAYLGMKMIIGAVKGWAKP
                     VSDTVAEPSLQSPNVFGRALILSLLNPKAILFFISFFVQFVDPSYPYPVVSFAVLAGI
                     LQVVSLTYLSVLIFSGAHLVRWFRSRHKIAAAGLASVGALFMSFAIKLWTATIA"
     gene            2247..>4308
                     /gene="carB"
                     /locus_tag="PK856_06330"
     CDS             2247..>4308
                     /gene="carB"
                     /locus_tag="PK856_06330"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRL02022.1"
                     /translation="MPKRTDLKTILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     VLVNSNPATIMTDPEMADVTYIEPIRWQTVEQIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLKKYNVELIGASEDAIDKAEDRGRFKLAMEKIGLHCPQSYVCHTMEESLAAQA
                     KVGYPTLIRPSFTMGGSGGGIAYNKEEFLEICERGFDASPTHELLIEQSILGWKEYEM
                     EVVRDKADNCIIICSIENFDPCGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFATNPETGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLNE
                     LHNDITGGRTPASFEPSIDYVVTKVPRFAFEKFPAADDRLTTQMKSVGEVMAIGRTFQ
                     ESVHKALRGLETGLAGFDTRSSDKSEIIREIGLPGPERILYVADAFRIGMTLEEIFEI
                     SKIDPWFLAQLRDLIEEETKLAAASLQDLDYTTLRRLKRKGFADQRMAMLLNVSETEV
                     RAHRHGLNLHPVYKRVDTCAAEFATDTAYMYSTYEEECEAQPSDRKKIMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     KVEQPYGLIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQQILKDLGLR
                     QPENR"
BASE COUNT         1041 a          975 c         1144 g         1148 t
ORIGIN      
        1 accgagtctt atgaatggac tgaaggcgag tggactttgg agcatggctt taagcggcct
       61 gtaaaccaac cttatcatgt ggtggcttat gattttggtg tgaaaaccaa tatcttgcgc
      121 atgttggccg aacgcggttg ccgcttgact gtggtacctg ctcagactcc tgccaaagat
      181 gtgttggctt tgaatcccga tggtgtgttt ttgtccaatg gccctggcga tcctgagcct
      241 tgcgattacg ccatcagcgc gattcaagac attttggcca gcaaaaagcc agtgtttggc
      301 atttgcttag gccaccaatt attgggtttg gcagtgggcg gtaaaacccg caaaatgcct
      361 tttggtcacc acggtgccaa ccatccagta caagatgtgg atgccaataa agtgatgatt
      421 accagtcaaa accatggttt tgaagtggat gaagccagct tgcctgccaa tgtgcgtgtg
      481 acccaccgtt cattgtttga tggctcggtg caaggcattg aattgaccga tcaagaggcg
      541 ttctcattcc aaggccaccc agaagcgagc cctggcccgc atgatgtggc gtatttgttt
      601 gaccgtttca ttaacgcgat gtctgccaaa taatcaacga aagcggcctg taaaccatga
      661 atgcaatgtc tttgcctacg gaattacatg attatggcta tgtattggtc aatgtgtttg
      721 cccccagtca ttttgcgggc aatccattgg cggttttccc gcatgccgat ggtttgagcg
      781 ataccgacat gcaagcgatt gccattcaaa tgaatttggc ggaaaccgtg ttcgcgttta
      841 aaagcgagga tgcggtggcg aaattgcgca tttttacgcc tgattacgaa ttgccgtttg
      901 caggacaccc tgtgattggt gctgcccgtg tgttagacca gctcaaacgc ttgcctgccg
      961 aatttaccgt gcaaaccgaa gcaggcatca cccagttaaa gcgcgacaaa atgcgttaca
     1021 ccttcacacg cacacaaggt gaatgtgatg aagtggcgag cagtgaagtg cctgccttat
     1081 tgatggcttt gggtttggat gacttgtctg tgtccagtgc gccgtgttgg gtcaatacag
     1141 gcagtgaaca attgctgctt gaagtgagcc aaaccgaagt gttaagccac atcaagcccg
     1201 atgctgaaca attgtcggcg tggtttgcta agcaaggcaa gcgtgcggcg gtgtatttgt
     1261 ggcatgaaaa aaaccgctat gtgcgtgtgc gttttttcag cgccgtgggc aatgtgttaa
     1321 ccgaagacat tggtacaggc tcggcttgtg cgaatttggg cggttggtgt ttggctcatg
     1381 gtttgaatga tgtgaactgg ttgatacagc aaggcgatta tttgcgcagg cccaaccgtt
     1441 tgcatttgca aaccagcgct gagggtttga tttatgtggg tggtgaaacc atcgtggtgg
     1501 gacacggcag tttgagtgtg ccgcacagcg ttaaggagca tgaaccatgt taggtgtgat
     1561 tgatttacct acctatgtga taggggtgat tgcgattatc ttgctgccag ggcctaactc
     1621 catgttttgc ctgactgtcg gtgcgcaata cggggtgaaa aaaggctata tcgcagcggc
     1681 aggcgtgttc attggtgact tggtgttgat gactttgtcg gcattgggtg cagcttcatt
     1741 gatgattaaa taccccgcga tttttaacat catcaaatat gtgggtggcg ggtatttggc
     1801 ttatttgggc atgaaaatga tcattggagc ggtcaaaggt tgggccaaac cagtgtctga
     1861 taccgtggct gagccgagtt tgcaaagccc caatgtgttt ggccgcgcgt tgattttgag
     1921 cctgctcaat cccaaagcga ttttgttctt catttcgttt ttcgtgcaat ttgttgaccc
     1981 aagctatccg tatcccgtgg tgagttttgc ggtgttggcg ggtattttgc aagtggtgag
     2041 tttgacttac ctgagcgtgt tgattttttc gggtgcacat ttggtgcgct ggtttcgcag
     2101 ccgtcacaaa attgcggcag caggccttgc ctctgtgggg gcattgttta tgagttttgc
     2161 cattaaattg tggacagcca caatagctta attagatttt aatgttacag gccgcttgta
     2221 tgcggcctga aagataagag attactatgc ctaaacgtac cgacttaaaa accattttga
     2281 tcataggcgc agggccgatt gtcatcggtc aagcgtgtga gtttgactat tctggcgcgc
     2341 aagcttgtaa agctttgcgt gaagaaggct ataaagtggt gttggtgaac tccaatccag
     2401 ccaccatcat gaccgaccct gaaatggccg atgtgactta catcgagccg attcgttggc
     2461 aaacggttga gcaaatcatt gccaaagagc gcccagatgc gattttgcct accatgggtg
     2521 gtcaaaccgc attgaactgt gccttggatt tggcccgcaa tggcgtgttg aaaaaataca
     2581 atgtggaatt gattggcgcc tctgaagacg ccatcgacaa agccgaagac cgcggtcgct
     2641 ttaaattggc gatggaaaaa atcggtttgc attgcccaca atcttacgtg tgtcacacca
     2701 tggaagaatc gttggcagct caagccaaag tgggctaccc aactttgata cgtccatcgt
     2761 tcaccatggg tggctctggc ggcggtattg cttacaacaa agaagaattt ttggaaatct
     2821 gtgaacgcgg tttcgatgcc tctcccaccc atgaattgtt gattgaacaa tcgattttgg
     2881 gctggaaaga atatgagatg gaagtggtgc gcgacaaagc ggacaactgc atcatcattt
     2941 gctctattga aaacttcgac ccatgtggcg tgcacacagg tgactccatc actgtcgctc
     3001 ctgcccaaac cttgaccgac aaagaatacc aaatcatgcg caatgcctct ttggctgtgt
     3061 tgcgtgaaat cggtgtggac actggcggtt ctaacgtaca gttcgctacc aaccctgaaa
     3121 caggcgaaat gattgtcatc gagatgaacc cacgtgtatc acgttcatct gctttggcat
     3181 cgaaagccac aggtttccca attgccaaag tagcggcgaa attggccgtg ggttacacct
     3241 tgaacgaatt gcacaacgac atcactggcg gccgtacgcc agcatcgttt gagccttcaa
     3301 tcgattatgt cgtgaccaaa gtgcctcgtt ttgcgtttga aaaattccca gccgcagacg
     3361 accgcttgac cacgcaaatg aaatcagtag gtgaagtcat ggccatcggc cgtaccttcc
     3421 aagagtctgt gcacaaagct ttgcgtggtt tggaaacagg cttggcgggt tttgataccc
     3481 gctctagcga caaatctgaa atcatccgtg aaatcggctt gcctggccca gagcgcattt
     3541 tgtatgtggc agatgccttc cgcatcggca tgactttgga agaaatcttc gaaatcagca
     3601 aaatcgatcc ttggttcttg gcacaattgc gtgacttgat tgaagaagaa accaaactgg
     3661 ctgcggcaag cttgcaagac ttggattaca ccactttgcg ccgtttgaaa cgcaaaggct
     3721 ttgccgacca acgcatggcc atgttgttga acgtgtctga aaccgaagtg cgtgcacacc
     3781 gtcatggttt gaatttgcac ccagtgtaca aacgcgttga cacttgtgcg gccgaattcg
     3841 caaccgacac cgcttacatg tattccactt atgaagaaga gtgtgaagca cagcctagcg
     3901 accgcaaaaa aatcatgatt ttgggcggtg gcccaaaccg cattggtcaa ggcattgagt
     3961 ttgactactg ctgtgtgcac gcggctttgg ctttgcgtga aagcggcttt gaaaccatca
     4021 tggtcaactg taaccctgaa accgtttcta ccgacttcga cacttcagac cgcttgtatt
     4081 tcgagccttt gactttggaa gacgtgttgg aaatcgtgaa ggtagaacaa ccttacggtt
     4141 tgattgtgca ttacggtggc caaacgccat tgaaattggc caatgccttg gttgaaaacg
     4201 gtgtgaacat catcggtacg tctgccgaca gcattgacgc tgccgaagac cgcgaacgct
     4261 tccaacaaat cttgaaagat ttgggcttgc gccaacctga aaaccgca
//
LOCUS       DAOMVK010000160         4302 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_5876_length_4302_cov_2.691076, whole genome shotgun sequence.
ACCESSION   DAOMVK010000160 DAOMVK010000000
VERSION     DAOMVK010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5876_length_4302_cov_2.691076"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PK856_06335"
     CDS             <1..297
                     /locus_tag="PK856_06335"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRL02023.1"
                     /translation="LTFEQSINTALNEVDGLYYSYRQSLAIEANLATKLAAEAKIKQY
                     YAARYKHGAAEFKDYIAAQTSYDSAYLAWLNGRYARLQAENKVYQAMAGRYRAN"
     gene            651..1682
                     /locus_tag="PK856_06340"
     CDS             651..1682
                     /locus_tag="PK856_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02024.1"
                     /translation="MNRLYFEPMKHVFEQMAERELWAFDMDLYHLNTPVNVVMYGLPD
                     SFADEDFKRVGMSGLKEFSQRLLAQIGFPAIDDATWNDGWWCYVLALEWLPVLDDADE
                     RSGNINRMWIWLGYDDNGEACEYRLLWTDSDSDSCAELMLGAQTMDERLMNSEFFSNN
                     IHKAAYIDLGIVLAAVVEYLGKPATDFAQWQYPSQLVPAPQPEDYVSLLQLLNPNTAE
                     ATLTASAAALHQRLIVEDHANEGDLAEWLLHENVFHHDWKFDAEDLSEVLADLLGQED
                     FVLDAPADTYGEDLFPCAQEALAEQQKCLMNYQSYGDSYYFFVLPSYQVETVLKLSQR
                     LGCDLEVLA"
     gene            complement(1899..2360)
                     /locus_tag="PK856_06345"
     CDS             complement(1899..2360)
                     /locus_tag="PK856_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HRL02025.1"
                     /translation="MHITDLNSGLQYATFVNTLPTMKKKTTLDVCPCLWQDGVLFADC
                     CGRYIAGTALAPTAEALMRSRYSAFVKEASDYLLQTWHPSTRPAAMDFEPTQWLGLKV
                     ISARDIDDSHAEVSFSARYKVNGRAHKMQENSRFVREQGAWLYVDGDVDTN"
     gene            2529..3443
                     /locus_tag="PK856_06350"
     CDS             2529..3443
                     /locus_tag="PK856_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HRL02026.1"
                     /translation="MSGDTIKVFVGCDPNNCDLEQMMVLDYSIHKHTQQPVEIVWMQL
                     SHDENSVWFSEPAKKIGWRTEKWATPFSGFRWAIPEYCGFEGRAIYMDADVVVLCDLA
                     ELWQHPMAAGAMVAGKGGQYNARLCTCVWDNVEAKKHLPALKDLQADPEGHHKLMQKL
                     KDQPQLVQAYQDTTYNCVDGEGLPIEQIKILHYSDMGTQFSHEFSFPRLQAEGMKHWF
                     DGAVMPHPREDLTELFRQYHAEALAAGYNPENYRYAAFGEFPKATQVNYSGNDVTRQK
                     EQKSALSRGWKKIKNSLSGKKDGFSLDH"
     gene            3768..3841
                     /locus_tag="PK856_06355"
     tRNA            3768..3841
                     /locus_tag="PK856_06355"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3801..3803,aa:Thr,seq:cgt)
     gene            3839..3908
                     /locus_tag="PK856_06360"
     tRNA            3839..3908
                     /locus_tag="PK856_06360"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3870..3872,aa:Gln,seq:ttg)
BASE COUNT         1068 a          905 c         1163 g         1166 t
ORIGIN      
        1 ttgacgtttg agcaaagcat taacaccgct ttgaacgagg tagacggcct gtattacagc
       61 taccgccaaa gcttggcaat agaggcaaac ttggccacca aactcgcggc tgaggccaaa
      121 atcaaacagt attatgcggc gcgctacaaa cacggtgcgg cggaattcaa agactatatt
      181 gctgcccaaa ccagttacga cagcgcttat ttggcgtggt taaacggccg ctatgcgcgc
      241 ttgcaagcag aaaacaaagt ctaccaagcg atggctgggc gttaccgcgc caattgatcg
      301 tccatgttct gaactgccag acagtgaggg gtattcttta tcagcggcct gtaagccttt
      361 acaggccgca ttttgttgac gcttaagcct gatgcctagt caccaagatt caaattggcc
      421 ttattctgtg gtcttaaata gaagcgatga gccgaatgag tttccaacaa tacaatggct
      481 tgagctgtga ttgcacatgg gggatatcgg caatgagcga gaggttgatg gcgaggcatt
      541 gaaataatct ggttttgtgg ctttaatgga catcatttgc gttgccgatg ttgaaaagag
      601 cctctctttg tctggccgcc ttattgaaac ctagtgtgag aaagttgtgc atgaacagac
      661 tgtattttga gcccatgaag catgtgtttg agcaaatggc tgagcgggaa ttgtgggcgt
      721 ttgacatgga tttataccat ttgaacacgc ctgtgaatgt ggtgatgtac ggtttgcccg
      781 attcgtttgc cgatgaggat ttcaagcgtg tgggcatgag tggtttgaaa gagttttcac
      841 agcgcttatt ggcgcaaatt ggatttcccg ccatagacga tgccacatgg aatgatggct
      901 ggtggtgtta tgtgttggca ttggaatggt tgccagtgct ggatgatgcg gatgagcgca
      961 gtggcaacat caaccgcatg tggatatggt tgggttatga cgacaatggt gaggcgtgcg
     1021 aataccgctt gttgtggaca gacagtgatt cagattcttg cgctgagttg atgctgggcg
     1081 ctcaaaccat ggatgagcgc ttgatgaatt ctgaattttt cagcaacaac atccacaaag
     1141 ccgcttacat cgatttgggc attgttttgg ccgccgtggt ggagtatttg ggcaagcccg
     1201 ccaccgattt tgctcagtgg caatacccat cgcagttggt gcctgcgcct cagcctgaag
     1261 actatgtgtc tttgctgcaa ttgctcaacc ccaacactgc cgaagcgact ttgacggctt
     1321 cagcggctgc cttacaccag cgtttgatag tggaagacca tgccaatgaa ggcgatttgg
     1381 ccgagtggtt gttgcatgaa aacgtgtttc accacgattg gaaattcgat gccgaggatt
     1441 tgagcgaagt attggcggat ttgctcgggc aagaagattt tgttttggat gctcccgcag
     1501 atacctatgg cgaagacttg ttcccctgtg ctcaagaggc cttggctgag cagcaaaaat
     1561 gcctgatgaa ttaccaaagc tatggcgaca gctattattt ctttgtcttg ccctcttatc
     1621 aagtggaaac agtgttgaaa ctcagccagc gtttgggctg tgatttggaa gtgttggctt
     1681 gagtgtggac attccatgat aaaagcggcc tgtaaacgct tacaggccgc ttttatcatg
     1741 atgagcttgg gtttgattga cactgcagat ggctacaatg catgagttgt cgcgatggga
     1801 taaggaatgc tatatatgag attgggctaa tgggtttggt tggtgaggct tctgtatgca
     1861 cctgtcagcg tttacaggcc gcctttattc tcaaaatctt agtttgtatc cacatcacca
     1921 tcgacatata gccatgcgcc ttgttcgcgc acaaagcggc tgttttcctg cattttgtgt
     1981 gcgcggccat ttactttgta gcgggcactg aagctaactt cggcatggct gtcatcaata
     2041 tcacgtgcag aaatcacttt caaacccaac cactgcgtgg gttcaaaatc cattgctgca
     2101 gggcgggtag acgggtgcca ggtttgcagc aaataatcgc ttgcttcttt cacaaacgcg
     2161 ctgtatcgcg agcgcatcaa agcctccgcc gtgggcgcaa gcgccgttcc tgcgatgtag
     2221 cggccacagc aatcggcaaa caaaacgccg tcttgccaca gacaagggca gacatccaaa
     2281 gtggtttttt tcttcatagt gggcagggta ttcacaaaag tggcatattg taagccagaa
     2341 ttgaggtcgg taatatgcat gcgtgacaag atgcatacgt gcagttcatg gaaaaaatca
     2401 acaaagggca aactgaacaa tgatgtaggt caaaccacag tgttcgggct ttaaaacaaa
     2461 caatgttata atcacgacca ttaaccaatg cttaacgcct gtcgatttgg gcgtatcagg
     2521 agacaatgat gagtggagac accattaagg tatttgtggg ctgcgatcca aataactgtg
     2581 atttagaaca aatgatggtc ttggactaca gcatacacaa gcatactcag cagcctgtgg
     2641 aaattgtgtg gatgcaattg agccatgatg aaaacagcgt gtggttttct gaacctgcta
     2701 aaaaaatcgg ttggcgcact gaaaaatggg caacgccgtt ttcgggcttt cgttgggcga
     2761 ttcctgaata ctgcggtttt gaaggtcgtg ccatttacat ggatgccgat gtggtggtgt
     2821 tgtgtgattt ggctgaattg tggcaacacc ctatggctgc gggtgcgatg gtggcgggca
     2881 agggtgggca gtacaatgcg cgtttgtgta cctgcgtgtg ggacaatgtg gaagccaaaa
     2941 agcatttgcc tgccttaaaa gacttacagg ccgatccaga gggccaccac aaattgatgc
     3001 aaaaattaaa agaccagcca caattggtac aagcctacca agacacgact tacaactgtg
     3061 tggatggtga gggcttgccc attgagcaaa tcaaaatcct gcattattct gacatgggca
     3121 cgcaattcag tcatgagttt tctttcccgc gcttgcaagc cgaaggcatg aagcattggt
     3181 ttgatggcgc ggtgatgcca cacccgcgtg aggatttgac cgagttgttc cgacaatacc
     3241 atgctgaggc attggctgcg ggctacaatc ctgaaaacta ccgctatgct gcctttggtg
     3301 agtttcccaa agccacgcaa gtcaattact caggtaacga tgtgacgcgc caaaaagagc
     3361 aaaaatcggc tttgtcacgc ggttggaaaa aaatcaagaa cagtttaagt ggtaagaaag
     3421 acggtttttc attagaccat taatgtctgc aatgcattga taacagctaa ttaaagcggc
     3481 ctgtaacttt tacaggccgt tttgtttgta tcaatccgtt tttttgattt aacaagacgt
     3541 ttcggagaag gctcttctgt gtggatttat aggtgtatgg gtatttgctt agttgtttaa
     3601 gaatggagat acattgagat tggtgtgcag taagttgtat gaatgcacta ttcactcggc
     3661 ttgcctaggc cgtataatcc atgccgtgtg attatggtct ttggttcagc tttcatggct
     3721 ggccggtgca ggcgaggtat cgttctgtat gccgaacatg gttgcatgga taatttagct
     3781 caatggtaga gcaatgaact cgtaattcat cggtagaagg ttcgactcct tcaattatgc
     3841 ggattagctt agcggttaaa gcccaactgt tgacagttgt gacggtggtt cgactccact
     3901 gttcgccatc agggatttat tcccacctga gcagtaaact ttggttgtaa tagcaatacc
     3961 ccgcggtctg ggcgttgtgg ctttgtgtgc attgatgttg gatgcagaca aactggtttt
     4021 cttgtctgtt ggcagcttga cgcagcggcg ggcagcaaaa cccaagtggt gtggctggtt
     4081 taggttggat gtgaagccca gcaccgattg tgtttgctgc ttcctgtggc gcacactgtt
     4141 tgaccacaag gcggcctgtg tccacagcgt ttggaccgcc catatttatt cgaggatgag
     4201 cacatgtttt ggcagttaaa aatcgatgta aaccctggtg aacgggtcat cgtgaccgat
     4261 aaggggcgct tactcagcgt attgactgct ggtgaacacc gc
//
LOCUS       DAOMVK010000161         4275 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_5941_length_4275_cov_2.912559, whole genome shotgun sequence.
ACCESSION   DAOMVK010000161 DAOMVK010000000
VERSION     DAOMVK010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4275
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5941_length_4275_cov_2.912559"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1240)
                     /locus_tag="PK856_06365"
     CDS             complement(<1..1240)
                     /locus_tag="PK856_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL02027.1"
                     /translation="MNAQLPVPLPKNPPPNFRRYLPLLLAMAIFMQMLDTTVLNTALP
                     AMAADLNVSPLNMQSVIVSYALTLALLIPLSGYLSARFGTQKVFLAAIGLFSFGSLLC
                     AFSPTLPTLVFSRIVQGVGGALLTPVARLALIKSFEKSELLRVLNFAIMPALMAPIMG
                     PLVGGYLVEWVSWHWIFLINLPVGLLCFLAGWRYMPNYTEQNTHIDVPGILLFGGAAF
                     FLTIGLEFLGSDNKALAFALLTLATGSALLFGYYRYAKTHKNAIYPLSLLSVRTFRVG
                     LNGNLLARLGISSIPLLLPLLLQVSFGYSPSEAGWMIAPMALAAMLTKPLMQKLLQTF
                     GYRNILLANTVLVGVLMMSIALHGPDSPKWLLVLHFFILGGCNSIQFTSMNTITLADL
                     RPYQNTSGNSLMAVNQQLAMG"
     gene            1457..>4275
                     /gene="putA"
                     /locus_tag="PK856_06370"
     CDS             1457..>4275
                     /gene="putA"
                     /locus_tag="PK856_06370"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HRL02028.1"
                     /translation="MFDFAKEQTPLRNKITDLYRIDEATAINQLASQLDLSQQSKQIE
                     DWARQLISQVRSKRQKMAGVDALMHEFSLSSEEGVALMCLAEALLRVPDKQTQTFLIR
                     DKLSRGDWKSHVGQGSSLFVNAAAWGLLLTGKLSQPANDQNLSSALNRMLSKGGEPMI
                     RKGMDYAMRMLGQQFVTGQTIDEALKNGHERETRGYRFSFDMLGEAALTQLDAERYYQ
                     DYINAIHAIGRDSKGRDVYDSNGISVKLSALHPRYNRNQHARVMNELLPKVKYLYLLA
                     KEYNIGLNIDAEEANRLELSLDLLEAIAQDEDLAGFNGIGFVVQAYQKRCPAVLDFII
                     DLARRTNHRFMIRLVKGAYWDSEIKIAQVDGLEGYPVYTRKVHTDVAYLACAQKLLGA
                     TDVIFPQFASHNAYTVAAIRALGGDKDFEFQCLHGMGETLYDQVVGEGNWKRRVRIYA
                     PVGTHETLLAYLVRRLLENGANSSFVNQIVDENIDIDSLVQNPLKQLEITGGQPHPAI
                     PLPINLYGASRTNSQGIDLSNEYRLQELQAELNQAGASQYQAASLTASAIEQQAVRPV
                     NNPANGQEQVGEVTYASASSVPAIIDAALTAQTAWQQKSASERAAILRKIADLMEEKQ
                     SALLYLAIKEAGKTLINAVGEIREAVDFCRYYAQQIEEFMPNHAAARGVIVAISPWNF
                     PLAIFVGQIVAALAVGNSVVAKPAEQTSLMAHAAVQLMLEAGVPSGVVQLILGEGDIG
                     AALTADPRIAGVLFTGSTEVAQLINRTLSKRSDMPLLLAETGGQNAMIVDSTALPEQV
                     VQDVLLSAFDSAGQRCSALRVLCVQDDIAERVIEMLKGGLKELNIGRPHDLDVDVGPV
                     IDAEAQQRLLEYIERKRSVAAKVTQLEIPSDINANGTYVAPTILELKNLGDLTQEVFG
                     PVLHVVRFKAGELDQLIAHINEQNYA"
BASE COUNT         1171 a         1061 c         1117 g          926 t
ORIGIN      
        1 aacccatggc caattgttgg ttgaccgcca tcaagctgtt gccactggtg ttttgatacg
       61 ggcgcaaatc ggcgagggta atcgtgttca tcgaagtgaa ttggatggaa ttacaaccgc
      121 ccaaaataaa gaaatgcaac accaacagcc atttcgggct gtctggacca tgcaaggcta
      181 tgctcatcat caacacgccc accaaaacgg tgttggcgag caagatattg cggtagccaa
      241 aggtttgcaa cagtttttgc atcaatggct tggtcaacat cgccgccaaa gccatgggcg
      301 caatcatcca gcccgcttca ctgggcgaat atccaaatga cacttgcagc aacaaaggca
      361 acaataaagg aatggagctg atgcccaaac gcgcgagcaa attgccgttt aaacccactc
      421 gaaacgtgcg caccgataac aggcttaagg ggtaaatggc atttttgtgg gttttggcat
      481 agcggtaata gccaaacagc aaagccgaac ctgtggccaa agtcagcaag gcaaaagcca
      541 gcgctttgtt atcgctgcct aaaaattcca atccgatggt gaggaaaaat gcggcgccgc
      601 caaacagcaa gattcctgga acatcgatgt gggtgttttg ttcggtgtag tttggcatgt
      661 agcgccagcc tgccaaaaag cacagcaaac ccacgggcag gttaatcaaa aaaatccaat
      721 gccaagacac ccattccacc aaatagccac ccaccaacgg gcccatgatt ggtgccatca
      781 aagcgggcat gatggcgaaa ttcaataccc gcagcaattc ggatttttca aacgatttaa
      841 tcaaagccaa gcgcgccaca ggcgtgagca aagccccacc tacaccttgc acaatgcgcg
      901 aaaacaccag cgtgggcagc gtgggcgaga acgcacacaa tagcgaacca aagctgaaca
      961 aacctatcgc agccaaaaac accttttgtg taccaaaacg ggcgctcaga taaccactaa
     1021 gaggaatgag caaggccaga gtcaacgcgt acgacacgat gaccgactgc atgtttaagg
     1081 gcgacacatt caaatcggcg gccatggcag gcaaggcggt attgagtacc gtagtgtcga
     1141 gcatttgcat gaagatggcc atggccaata acagcggcaa atagcggcga aaatttggcg
     1201 gtgggttttt gggcaggggc accggaagtt gcgcattcat gaagctgcct ctacaaaaaa
     1261 agggacaaac tcacattcta gccgcaagca aaaacaagat aaagtttcat tccagtataa
     1321 ctggattact tattgataaa gttttaatcc aagcgattga caggcagtat ttttgcaagc
     1381 aatgtaacgg ttttacggcg acgaaatcgc cgtatttgtg cacagggcac acgacgacac
     1441 caaaggagca agttaaatgt ttgattttgc caaagagcaa acaccattac gcaataaaat
     1501 caccgatttg taccgcattg atgaggccac tgccatcaat caattggcca gccaattgga
     1561 tttaagccaa caatccaagc aaattgagga ttgggcgcgt caattgattt cacaagtgcg
     1621 ctccaagcgc caaaaaatgg cgggtgtcga tgccttgatg catgaattca gcttgtcgag
     1681 tgaagaaggt gtggcgttga tgtgtttggc agaggctttg ctccgcgtgc ctgacaaaca
     1741 aacccaaacc tttctcattc gtgacaaatt gtcgcgtggc gattggaaga gccatgtcgg
     1801 tcaaggctct tctttgtttg tgaacgctgc cgcttggggc ttgttgctca caggcaaatt
     1861 gtcacaacct gccaacgacc aaaatctgag cagcgcactg aaccgcatgc tcagcaaagg
     1921 cggcgaaccg atgatacgca aaggcatgga ctacgccatg cgcatgttgg gccagcaatt
     1981 tgtgacaggt caaaccatag acgaagcctt gaaaaacggc cacgagcgcg aaacccgtgg
     2041 ctaccgtttc tcatttgaca tgttgggcga agcggcttta acccaattgg atgccgaacg
     2101 ctattaccaa gactacatca acgctataca cgccatcggt cgcgacagca aaggccgcga
     2161 tgtctacgac agcaacggta tttcagtcaa actgtcggct ttgcacccac gctacaaccg
     2221 caaccaacac gcccgcgtga tgaatgaatt gttgcccaaa gtcaaatacc tgtatttgtt
     2281 ggcgaaagag tacaacatcg gcctgaacat cgatgccgaa gaagccaacc gcttggaatt
     2341 gtctttggat ttgttggaag ccatcgccca agacgaagat ttggcgggct ttaatggcat
     2401 cggttttgtg gtgcaagcct atcaaaagcg ctgtcctgcg gtgttggatt tcatcattga
     2461 tttggcacgc cgcaccaacc accgctttat gattcgtttg gtaaaaggcg catattggga
     2521 cagcgaaatc aaaatcgccc aagtcgatgg cttggaaggc tatcctgttt acacccgcaa
     2581 agtgcacacc gacgtggcat atttggcttg cgcacaaaaa ttattgggcg caaccgatgt
     2641 gatcttcccg cagtttgcat cacacaacgc ctataccgtg gcggccatcc gcgctttggg
     2701 tggcgataaa gactttgaat tccaatgctt acacggcatg ggcgaaaccc tgtacgacca
     2761 agtggtgggc gaaggcaatt ggaaacgccg cgtgcgcatt tacgcgcctg tcggcaccca
     2821 tgaaaccttg cttgcttact tggtgcgccg cttattggaa aatggtgcta actcatcatt
     2881 tgtgaaccaa atcgtcgatg aaaacatcga catcgattct ttggtgcaaa acccattgaa
     2941 acagctagaa attacgggtg gtcaaccaca tcctgctatt ccattgccta tcaatttata
     3001 tggcgcgagc cgcaccaatt cgcaaggcat agacttgagt aatgagtacc gcttgcaaga
     3061 attacaggct gagttaaacc aagcgggagc gagccaatac caagccgcat ccttaaccgc
     3121 cagcgcgata gagcagcaag cggtgcggcc tgtaaacaat cctgccaatg ggcaagagca
     3181 agtgggcgaa gtgacctatg cttcggccag cagcgtgcct gccatcattg atgcggcgct
     3241 gactgcgcaa acggcttggc agcaaaaatc ggccagtgag cgcgccgcca tcttgcgcaa
     3301 aattgccgac ttgatggaag aaaaacaaag cgccttgctg tatttggcga ttaaagaagc
     3361 aggcaaaact ttaatcaatg ccgtgggtga aattcgtgaa gcggtggatt tctgtcgcta
     3421 ttacgcgcag caaatcgaag aattcatgcc caaccatgcg gcggcgcgtg gcgtgattgt
     3481 ggcgatcagc ccgtggaatt tcccattggc gatttttgtg ggccaaatcg tggcagcttt
     3541 ggccgtgggc aacagcgtgg tggccaagcc tgccgaacaa acttcgctga tggcacatgc
     3601 ggcggtgcag ttgatgcttg aagcaggtgt gccaagcggt gtggtgcaat tgattttggg
     3661 cgagggcgac attggcgcgg ccttaaccgc cgacccacgc attgcaggtg tgctcttcac
     3721 aggctctact gaagtggcac aattgattaa ccgcaccttg tccaaacgca gcgacatgcc
     3781 tttactgctg gcagaaacag gtggccaaaa tgcgatgatt gtggacagca ccgccttgcc
     3841 tgaacaagtg gtgcaagacg tgttgttgtc ggcgtttgat tcggcaggtc agcgctgctc
     3901 ggcactgcgt gtgttgtgtg tgcaagacga cattgccgag cgtgtgattg aaatgctcaa
     3961 aggcggcctg aaagaattga acattggccg tccgcatgat ttggatgtgg atgtgggccc
     4021 tgtgattgat gcggaagcgc agcaacgctt gttggaatac atcgagcgca aacgctcggt
     4081 ggcagccaaa gtcacccaat tggagattcc tagcgacatt aatgccaacg gcacttacgt
     4141 ggcaccgacg attttggaac tgaaaaacct cggcgatttg acccaagagg tgtttggccc
     4201 cgtgctgcat gtggtgcgct ttaaagcggg tgaattagac cagttgattg cccacatcaa
     4261 tgagcaaaac tacgc
//
LOCUS       DAOMVK010000162         4265 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_5960_length_4265_cov_3.763658, whole genome shotgun sequence.
ACCESSION   DAOMVK010000162 DAOMVK010000000
VERSION     DAOMVK010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5960_length_4265_cov_3.763658"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="PK856_06375"
     CDS             complement(<1..606)
                     /locus_tag="PK856_06375"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRL02029.1"
                     /translation="MPRTQIRAEMMLALATLGWGMNYPLMKMALQHELGIWTLAVRFS
                     LSFLIMLPLLWMQRQQLSWGSIKVGCILGLILLPSVELMNLGLHYTSSANAGFIFALC
                     IIWVPILNAYLNKTRISPQIKFSMTLGLIGLTIIANLHQLKFNYGDFLILLCSFLYAA
                     YILVLDRYGKTYSGIVMTVMQLLVLGVGCTVIYAIAKPPVHV"
     gene            1088..1747
                     /locus_tag="PK856_06380"
     CDS             1088..1747
                     /locus_tag="PK856_06380"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HRL02030.1"
                     /translation="MKKVLINVSIVVGMLLLYFVPSILMGLSMLVATKHVGESFVWFR
                     DLAPFLGMLIASIGLLYWAYTLGWFRNLNWRIGGKGWLHLIGVFVLMQVWVMAFSQFK
                     PEHTDNDKAIMELMDLFPLWLMFVFICIVAPICEEIIFRGGLIGQIFAKHLCWGAAVN
                     TLLFAAIHAPEDAISWLMYGGLGAAFSWVYVRTQQLWQCVVLHMANNLLALASMLGWL
                     G"
     gene            1821..3467
                     /locus_tag="PK856_06385"
     CDS             1821..3467
                     /locus_tag="PK856_06385"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRL02031.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILEARGLKVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFINSTMHKSNNFTTGQVYEEVLAKE
                     RRGDYLGGTVQVIPHITDEIKRKVHVGAADNQVAIVEIGGTVGDIESLPFLESIRQMR
                     SQLGRANTLFVHLSYVPYIEAAGEIKTKPTQHSVKEMRSIGLQPDILVCRLKNMLPEE
                     EKRKIALFCNVEERAVISCPDEDSIYKIPELLHNQGIDKIICEQLQLNVAEADLTQWK
                     KIVHAINNPKHTVKIGMVGKYVDLTESYKSLSEALKHAGIHTETNVDITYFDSEQIEK
                     EGTDALLAMDAVLVPGGFGVRGVEGKIKAVEYARTNKVPYLGICLGMQIALIEYARHE
                     ANMEGANSTEFDLKTKFPVVALVEEWVNEDGQIEKRDENTDLGGTMRLGAQTCELNEG
                     SLAARIYGATEIKERHRHRYEVNGQFIPKLEEAGLVIAGYSSGHEHLVETIELPQDVH
                     PWFFASQFHPEFTSTPRKGHPLFTAFVKAALKSKVANGDK"
     gene            3547..>4265
                     /gene="kdsA"
                     /locus_tag="PK856_06390"
     CDS             3547..>4265
                     /gene="kdsA"
                     /locus_tag="PK856_06390"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012364209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HRL02032.1"
                     /translation="MKLCGFDVGLDQPFFLIAGPCVIESEQMALDTAGYLKEITTELG
                     IPFIYKSSFDKANRSSGTSFRGLGMAEGLRILDEVKRQIGVPILTDVHTEAEVPEVAA
                     VVDVLQTPAFLARQTDFIRAVAQSGKPVNIKKGQFMAPEDMGNVIDKARAAAAEKGLP
                     ADFMVCERGASFGYHNLVSDMRSLMIMRDTDCPVVFDATHSVQMPGGLGNASGGRREF
                     VPVLARAAIAAGVAGVFMETHP"
BASE COUNT         1112 a          922 c         1116 g         1115 t
ORIGIN      
        1 gacgtgtaca ggtggcttgg cgatggcgta aatcaccgta cagcccacac ccaataccaa
       61 tagctgcatc acggtcatta caatgccaga ataggttttg ccatagcgat cgagtaccaa
      121 aatgtacgct gcatacaaga atgagcacaa taaaatcagg aaatcgccgt agttgaattt
      181 cagttggtgt aaattggcga tgatggtcaa gccaatgagg cccaaagtca tgctgaattt
      241 gatttgtgga ctgatgcggg ttttgttcaa gtaggcattt aaaatcggta cccaaatgat
      301 gcacagagca aaaatgaacc ctgcattggc tgagctggtg taatgcaggc ccaaattcat
      361 taattcaact gaaggcaaga ggattaagcc caaaatacaa cccactttga tgctgcccca
      421 agacaattgt tggcgctgca tccacaacag cggcaacata atgagaaaac tgaggctaaa
      481 acgcactgcc aaagtccaaa tgcccagctc gtgttgtaaa gccattttca tcaaaggata
      541 gttcatgccc caacctaagg tggcaagagc gagcatcatt tcagcgcgaa tttgggtgcg
      601 tggcatggga gtcaatttct gaattattgt gttaagggtt gacgtataaa gtgtgatttc
      661 attgtagcga cagatgaggg ctttgtgtca tcaggtgcta tctaaatttt agattatttt
      721 ttacttttca gacttattga tgaagtacaa cagatgggtg tgtagctatc attggtgttt
      781 ggtgggaaag ataaaatgtg tgattgtagg cactacaggt ggggtgtgta tgcaaagcga
      841 aatgatggtt tgagaactta cttagcagct aagaaattaa catgtggtca ttcaatatag
      901 ctattgggac aaggaaatat cacgcactgt gtttacagac cgctcttatc ttatgatgcg
      961 acctgtaagc aggctagagc ttgagtaatg tcatgcgagg cggtattctc agctgaattt
     1021 cagtatggct gatggccttg catcgccatt tctcgcctaa tctctaattt aagatgtgaa
     1081 agtcaaaatg aaaaaagtcc tcatcaatgt ttccatcgtc gtaggcatgt tgttgctgta
     1141 ttttgtgccg tcaattttga tgggcttgtc catgttggtg gcgaccaagc atgtggggga
     1201 gagttttgtt tggtttcgag acttggcgcc tttcttgggg atgttgattg cgagcatagg
     1261 cttgttgtat tgggcgtaca ccttgggttg gtttcgcaat ttgaattggc gcataggcgg
     1321 caaaggatgg ctgcatctga taggggtgtt tgtgttgatg caggtgtggg tgatggcgtt
     1381 cagtcagttc aagccagagc acacggataa cgacaaagcc attatggaat tgatggattt
     1441 gtttccattg tggttgatgt ttgtgtttat ttgtatagtc gctccgatat gcgaggaaat
     1501 cattttccgt ggcgggctca ttggccaaat ttttgccaag catttgtgtt ggggtgcggc
     1561 agtcaataca ctgttgtttg cggcgataca tgcacctgaa gacgccatca gttggttgat
     1621 gtacggtggt ttgggcgcag cgttttcatg ggtgtatgtg cgcacccagc agttgtggca
     1681 atgcgtggtt ttgcacatgg cgaacaattt attggccttg gccagcatgc tgggctggtt
     1741 ggggtaagta ttcgtcaatc tgggtttaag tccgatttca gtcatgctat aattcgcaat
     1801 tccattcagt gtattctgtc atgaccaagt ttatctttgt gaccggcggt gtcgtttctt
     1861 ctttaggtaa aggcattgct gcagcgtcca tcgctgcgat tctcgaagcg cgtggcttaa
     1921 aagtcaccat gctcaaacta gacccttaca ttaacgtaga cccaggcacg atgagcccgt
     1981 ttcaacacgg cgaagtgttt gtgaccgaag acggcgccga aaccgacttg gatttgggcc
     2041 actacgagcg tttcatcaat tccaccatgc acaagtctaa caacttcacc acgggtcaag
     2101 tgtatgagga agtgttggca aaagagcgtc gcggtgatta tttgggcggc acggtacaag
     2161 tgattccgca catcaccgat gaaatcaaac gcaaagtgca tgtgggcgct gctgacaacc
     2221 aagtggcgat agtggaaatc ggtggcacgg taggcgacat tgagtctttg ccattcttgg
     2281 aatccatccg ccaaatgcgc agccaattgg gtcgcgccaa taccttgttt gtgcacttgt
     2341 cttacgtgcc ttacattgaa gccgcgggtg aaatcaaaac caaaccgacc caacactctg
     2401 tgaaagagat gcgctcgatt ggtttgcagc cagacatttt ggtgtgtcgc ttaaaaaaca
     2461 tgttgcctga agaagaaaaa cgcaagattg ccttgttctg taatgtggaa gagcgagccg
     2521 taatcagttg ccctgatgaa gacagcattt acaaaattcc agaattgctg cacaaccaag
     2581 gcatagacaa aatcatttgt gaacaattgc agttgaatgt ggccgaagcc gatttaaccc
     2641 aatggaaaaa aattgttcac gccatcaaca acccgaaaca caccgtgaaa atcggtatgg
     2701 tcggcaaata cgttgatttg accgagtctt acaagtcttt gtctgaagcc ttgaaacacg
     2761 caggcatcca cactgaaacc aatgtggaca tcacttattt cgacagtgag caaattgaaa
     2821 aagaaggcac tgatgccttg ttagccatgg atgctgtttt ggtacctggt ggttttggtg
     2881 tgcgcggtgt cgaaggcaaa atcaaagccg tagaatacgc ccgtaccaac aaagtgcctt
     2941 acttgggcat ttgcttgggc atgcaaatcg ccttgattga atacgcacga cacgaagcga
     3001 acatggaagg cgccaactct accgaattcg acttgaaaac caaattccca gtagtggctt
     3061 tggtggaaga gtgggtgaat gaagacggtc aaattgaaaa acgcgatgaa aacaccgacc
     3121 tgggtggcac catgcgtttg ggcgcgcaaa cctgcgaatt gaacgaaggc tcattggccg
     3181 cgcgcattta cggcgccacc gaaatcaaag agcgtcaccg tcaccgctat gaagtgaacg
     3241 gccaattcat tcccaaattg gaagaagcag gcttggtgat tgcaggttat tcaagtggcc
     3301 acgagcattt ggtggaaacc atcgaattgc cacaagacgt acacccatgg ttcttcgcct
     3361 cgcaattcca tcctgaattc acatcaacgc cgcgcaaagg ccatccgctc tttactgcgt
     3421 ttgtgaaagc agctttgaaa agcaaagtcg ccaacggtga taaataagcg ctaaactgtt
     3481 acactcactg ccaatggtta tgcactgttg gcagtttgtt tttcagcacg tctgaaagga
     3541 aaaaacatga aattatgtgg ttttgacgtt ggtttagacc aaccattttt cttaatcgct
     3601 ggcccatgtg tgattgaaag cgagcaaatg gcgctggaca cagctggcta tttgaaagaa
     3661 atcaccactg agttgggcat tccgtttatt tacaaatcca gttttgacaa agccaaccgc
     3721 tctagcggca cctcattccg cggcttgggc atggccgaag gcttgcgcat cttggacgaa
     3781 gtgaagcgcc aaatcggtgt gccgattttg accgatgtgc acaccgaagc cgaagtgcca
     3841 gaagtggccg ccgtcgtgga tgtgttgcaa acgcctgctt tcttggcgcg ccaaaccgat
     3901 tttattcgcg cggtggccca atcaggcaaa ccggtgaaca ttaaaaaagg ccaattcatg
     3961 gcgcctgaag acatgggcaa tgtcatcgac aaagcccgtg cagccgctgc cgaaaaaggt
     4021 ttacccgctg actttatggt gtgtgagcgt ggcgcaagct tcggctacca caacttggta
     4081 tccgacatgc gcagcctgat gatcatgcgc gacaccgatt gtcctgtggt atttgatgcc
     4141 acgcattcgg tgcaaatgcc tggcggtttg ggcaatgctt cgggtggtcg ccgtgaattt
     4201 gtgcctgtgc tggcgcgtgc ggccatcgcc gcaggtgtgg cgggcgtgtt catggaaacc
     4261 catcc
//
LOCUS       DAOMVK010000163         4254 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_5978_length_4254_cov_3.638485, whole genome shotgun sequence.
ACCESSION   DAOMVK010000163 DAOMVK010000000
VERSION     DAOMVK010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5978_length_4254_cov_3.638485"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PK856_06395"
     CDS             complement(<1..316)
                     /locus_tag="PK856_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRL02033.1"
                     /translation="MKILSLRFENLNSLYGKWSVDFQHPDYTDNGLFAIVGPTGAGKS
                     TLLDAICLGLFGQTPRLKTISKSENEIMSRHSGYCSAEVVFAVGDKTYRSTWSQSRAR
                     NKP"
     gene            complement(313..1542)
                     /locus_tag="PK856_06400"
     CDS             complement(313..1542)
                     /locus_tag="PK856_06400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D C-terminal
                     domain-containing protein"
                     /protein_id="HRL02034.1"
                     /translation="MLKILHTSDWHLGRSLYGQNRQAEFNAFLNWLMDLLQQERTDVL
                     LIAGDVFDTITPNHAAQELYYRFLAQIAALSLCQHVVIIGGNHDSASFLNAPKSLLSA
                     LNVHVIGASTALEQEVLVLKDHDDKASAIVCAVPYLRDRDVRMSVDGESSQDKNLALV
                     HGIKAHYRDALNLAKHKQQEMGSDVPIIAMGHLFTQGGVTLDADGVRELYVGSLAHIG
                     ADCFDGYDYVALGHLHVPQTVGKQEHIRYCGSPIPMGFGEAKQQKQVIQVTFTGRLPE
                     IQTINIPCFQSLQRIQGDLSHIQAELRQLMQASSNAYVEVVYQGNEVVTNLSEQLEAL
                     VLGSDLRLLRVYNRQFVNKVLVSAHDEAALPDLNPTEVFEVCMAQHELNEAQKSQLRD
                     SYQLVLHQVEQPEEDEA"
     gene            complement(1868..>4254)
                     /gene="gyrB"
                     /locus_tag="PK856_06405"
     CDS             complement(1868..>4254)
                     /gene="gyrB"
                     /locus_tag="PK856_06405"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HRL02035.1"
                     /translation="TQPEEYGADSIKVLKGLDAVRKRPGMYIGDTQDGSGLHHMVFEV
                     LDNAIDEALAGHCDTIVVTIHSDNSISVEDNGRGIPTDMHPEENRSAAEVIMTILHAG
                     GKFDNNSYKISGGLHGVGVSVVNALSDWLTLNIYRNGVEHFVEFNRGDVVAPLKAIGD
                     TSKKGTRVQFLASEETFGVVEFNFDILAKRIRELSFLNNGVAIQLLDKRDGRHEDFAF
                     SGGVGGFVKYINRTKTALHDKIFYTQGESEDGMSVEVAMQWNDSYQEQVLCFTNNIPQ
                     RDGGTHLTALRQVMTRTLNQYIDDNEIAKKSKVQTAGDDMREGLTCVLSVKLPDPKFS
                     SQTKDKLVSSEIGPIVNEVINAGLKDFLEENPNEAKIITSKIVDAARAREAARKAREI
                     TRRKGIMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAKQGRDRKFQAILPLKGK
                     ILNVEKARFEKMLASQEVATLITALGCGIGKEEYNPDKFRYHRIIIMTDADVDGAHIR
                     TLLLTFFYRQMPEIIERGYVYIAQPPLYKAKHGKQERYLKDDFELNQFLLNLALQNAR
                     IESDGKTLEGEELANIAKQFMLTRGVINRESRAVDRSVLVQLVYLPALSFATYEDLLA
                     AEKLLQDSITDPDILIEAIPGDEHHLGKLKVTRRLHGNVMVTYLTQGFLESKDYQSIK
                     QMALLLDGLVHEGAVLFKGEKQQPVKSFSQALDWLLADAQKGLSIQRYKGLGEMNPEQ
                     LWETTMDPQVRRLLKVKIDDAIAADEVFVTLMGDEVEPRREFIESNALTAEIDI"
BASE COUNT          969 a         1043 c         1098 g         1144 t
ORIGIN      
        1 cgggtttgtt gcgggcgcgg ctttggctcc aagtgctgcg gtaggttttg tcgcctacgg
       61 caaacaccac ttcagccgaa caatagccgc tgtggcgcga catgatttcg ttttcgcttt
      121 tgctgatggt cttcaggcgc ggggtttgac caaacaagcc caagcaaatg gcatccaata
      181 gggtggattt gcccgctcct gttggcccca caatcgcaaa caagccattg tcagtgtaat
      241 cagggtgttg aaagtctact gaccacttgc catacagtga attcaggttt tcaaaacgca
      301 aactgaggat tttcatgctt cgtcctcctc aggttgctcg acttggtgca acaccagttg
      361 gtaactgtcg cgcaattggc ttttttgggc ttcgttcaat tcatgttgcg ccatgcacac
      421 ttcaaacacc tcggtgggat tcaaatcggg cagggcggct tcatcgtggg cgctgacaag
      481 gactttgttg acaaactggc ggttgtacac gcgcagtaag cgcaaatcgc tgcccaaaac
      541 gagggcttcg agctgttcac tcaaattggt cacgacttcg ttgccttggt aaaccacttc
      601 cacatacgca ttgctgcttg cctgcatgag ctgacgcaat tctgcctgta tgtgggacaa
      661 atcaccttgt atgcgttgca aagattgaaa gcaaggaatg ttgatggttt gaatttcagg
      721 caagcggcct gtaaacgtga cctggatgac ttgtttttgc tgcttggctt cgccaaaacc
      781 catggggatg ggcgagccgc aatagcgtat gtgttcttgc ttgccaacag tctgtggcac
      841 atgcaaatgc cctagcgcca cataatcgta gccgtcaaaa caatcagccc cgatgtgagc
      901 gaggctgccc acatacaatt cgcgcacacc atctgcgtcc aacgtcacac caccttgggt
      961 gaacaaatgc cccatggcga tgatgggcac atcgctgccc atttcctgtt gtttgtgctt
     1021 ggcgaggttt aaggcatcgc ggtaatgggc tttgatgcca tggactaagg ccaggttttt
     1081 atcttggctg ctttcgccat ccacactcat gcgcacatcg cggtcgcgca aataaggcac
     1141 ggcgcagaca atggcactgg ccttgtcgtc atggtctttg agcaccaata cttcttgttc
     1201 gagggcggtg cttgcgccga tgacatgcac gtttaaggca gacaacagtg atttgggggc
     1261 atttaaaaaa ctggctgaat catggttgcc accgatgatg acaacatgtt gacacaagct
     1321 gagtgccgca atctgagcca agaagcggta atacagttct tgtgcggcat ggttgggggt
     1381 aatggtgtca aacacgtcgc ccgctatcag caacacatca gttcgctctt gttgcagcaa
     1441 gtccatgagc caatttaaaa aagcgttgaa ttctgcctga cggttttgcc catacaggct
     1501 gcggcctaag tgccaatcgg aggtgtgtaa gatttttagc ataacccaaa tcaatgtgta
     1561 acgattttaa attggcccaa tacgaacagt cattctctca agttgccgag tataggtaag
     1621 ccatggcaat aatcataggg tctgttgcaa aagaccaagc catggttgag acacttgatt
     1681 tcgctggtga aaccttgttt ttgtgcatgg attctatcat gaattgtgca taaaacgggc
     1741 tggtcgcgtg tgcttcatcg agtgtgtcgc aagcaaagag ccgagtgctt gcaattgcga
     1801 aatactcaaa tacatacaaa caagcggcct gtaaatgttt acaggccgct tgtttgctat
     1861 cacatgctta aatgtcgatt tctgctgtca aagcattgct ttcaatgaat tcgcgacgcg
     1921 gctctacttc gtcgcccatc aaggtgacaa acacttcatc ggcagcgatg gcatcgtcga
     1981 ttttcacttt caacaaacgg cgcacttgtg ggtccatggt ggtttcccac aattgctctg
     2041 ggttcatctc gcccaagcct ttgtagcgct gaatcgataa gcctttttgg gcatcggcca
     2101 acagccaatc caaagcttga gagaaggatt tcactggctg ttgtttctcg cctttgaaca
     2161 ataccgcgcc ttcatgtacc aagccgtcga gcaacaaagc catttgtttg atggattggt
     2221 agtctttgct ttccaagaag ccttgggtga ggtaagtcac catcacattg ccgtgcaaac
     2281 ggcgtgtgac tttcagcttg cccaaatggt gttcgtcgcc agggatggct tcaatcaaga
     2341 tgtcagggtc ggtgatgctg tcttgcaaca gtttttctgc tgcgagcaag tcctcatacg
     2401 tggcaaaaga aagtgctggc aaatacacca attgcaccaa taccgagcgg tctaccgcac
     2461 ggctttcacg gttaatcacg ccgcgggtga gcatgaattg tttggcaata ttggccagct
     2521 cttcgccttc caaggtttta ccatcggatt cgatgcgggc gttttgcaaa gccaaattca
     2581 acaagaattg attcagctca aaatcgtctt tgaggtagcg ctcttgcttg ccatgtttgg
     2641 ctttatacag cggtggctgt gcaatgtaga catagccacg ctcaatgatt tcaggcattt
     2701 gacggtagaa gaaggtcaag agcaaggtgc ggatgtgcgc gccatcgacg tcggcatcgg
     2761 tcatgatgat gatgcggtgg tagcggaact tgtctgggtt gtattcttct ttgcctatgc
     2821 cgcagcccaa agcggtaatc aaggtggcca cttcttggct ggcgagcatt ttttcaaaac
     2881 gggctttttc cacgttcaaa atcttgcctt ttaacggcaa aatggcttgg aacttgcggt
     2941 cgcggccttg cttggctgag ccacccgccg aatccccttc gacgaggtag agttcagaca
     3001 gggcagggtc tttttcttgg cagtcggcca atttgcctgg caagcccaag ccgtccatga
     3061 tgcctttgcg gcgggtgatt tcacgcgctt tgcgcgcggc ttcacgcgca cgggcagcat
     3121 cgacgatttt gctggtgatg attttggctt cgttgggatt ttcttccaag aagtctttca
     3181 agcctgcgtt aatcacctca ttaacgatgg ggccaatttc gctggaaacc aatttgtctt
     3241 tggtttgcga gctgaatttc gggtcgggca atttcaccga caacacacaa gtcaagcctt
     3301 cacgcatgtc gtcacccgcg gtttgcacct tggatttttt ggcgatttcg ttgtcgtcta
     3361 tgtattggtt taaggtgcgc gtcatcactt ggcgcaaggc agtcaaatgg gtgccgccat
     3421 cgcgttgcgg gatgttgttg gtgaaacaca atacctgttc ttggtaagag tcgttccatt
     3481 gcatcgccac ttccacgctc atgccgtctt ctgattcacc ttgggtgtag aagattttgt
     3541 cgtgcaaagc ggttttggtg cggttgatgt atttgacaaa gccgcccacg ccgcctgaaa
     3601 acgcaaagtc ttcatggcgg ccgtcgcgtt tgtccaataa ttgtatggcc acgccattgt
     3661 tcaagaaaga caattcgcgg atgcgtttgg ccaaaatatc gaaattgaat tcgaccacgc
     3721 caaaagtttc ttcgctggcc aaaaattgca cgcgtgtgcc ttttttggag gtgtcaccga
     3781 tggctttcaa tggggcaacc acgtcgccgc ggttgaactc cacaaagtgt tccacaccat
     3841 tgcggtaaat attgagggta agccaatcgg acaaggcatt caccactgac acgcctacgc
     3901 cgtgcaagcc gccagagatt ttgtaagagt tgttatcgaa tttaccgcct gcgtgcaaaa
     3961 tggtcatgat gacttcggca gcagagcggt tttcttcagg atgcatgtcg gtaggaatgc
     4021 cgcgaccgtt gtcttcaact gagatggaat tgtcagaatg aatggtgacc acgatggtgt
     4081 cacaatggcc ggccaaggct tcatcgatgg cattgtcgag cacctcgaat accatgtgat
     4141 gcaagccgct gccatcttgg gtgtcgccaa tgtacatgcc tgggcgtttg cgtaccgcat
     4201 ccaaaccttt taacacttta atgctgtcag caccgtattc ttctggttga gtca
//
LOCUS       DAOMVK010000164         4217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_6039_length_4217_cov_3.582172, whole genome shotgun sequence.
ACCESSION   DAOMVK010000164 DAOMVK010000000
VERSION     DAOMVK010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6039_length_4217_cov_3.582172"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..772)
                     /locus_tag="PK856_06410"
     CDS             complement(86..772)
                     /locus_tag="PK856_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRL02036.1"
                     /translation="MKAMILAAGRGERMRPLTDATPKPLLKVGREPLIGWHLRRLQQA
                     GFTEVVINHAWLGQQLEDVLQDGRDYGLHIVYSPEGAQGLETAGGIATALPLLGKEPF
                     LVINGDVLTDIDFAQAEHAAAQMAKQQSLAHLWLVNNPEHNPNGDFGLDGEHVVSHAP
                     QQYTFSGCGIYHPDLFQHTPARQAAKLAPLLREAMNHQQVTGSLHTGLWLDVGTVERL
                     QQANDIMQHA"
     gene            1099..2154
                     /gene="aroG"
                     /locus_tag="PK856_06415"
     CDS             1099..2154
                     /gene="aroG"
                     /locus_tag="PK856_06415"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009119503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HRL02037.1"
                     /translation="MKNTDDVKIKEIKELLPPIAHLYELPLTEPASTLVHDTRAEISD
                     LIHGKDQRLLVVVGPCSIHDPKAALEYAERLMPLKKKYEKELVIVMRVYFEKPRTTIG
                     WKGLINDPHLDGSFDINFGLRQARHLLLELNNMGMPASTEFLDMITPQYYADLISWGA
                     IGARTTESQVHRELASGLSCPVGFKNGTDGNMKIAIDAIGAASNPHHFLSVTKSGHSA
                     IVRTAGNPDCHVILRGGSKNTNYDSESIQSAVAELSKAGVTPKLMVDCSHANSRKKYE
                     LQMEVGRDVAEQIKVGESNLMGVMIESHLVEGRQDLVEGQELCYGQSITDACIGWGDT
                     EVLLAELADAVKTRIAA"
     assembly_gap    2217..2226
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2381..3130
                     /locus_tag="PK856_06420"
     CDS             2381..3130
                     /locus_tag="PK856_06420"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003822318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HRL02038.1"
                     /translation="MLQLQTQSHVYIQGGSLSVSLARSEFEIEEAQRLRYKVFAEEMG
                     ANIVSDNGLDTDEYDDYCQHLLVRERENDRIIGCYRLLTAEGAVAIGNWYSANEFDLS
                     RLQHILPQAVELGRACVHQDYRSGSTITLLWAGLMQFMQQQGLEYMIGCGSMCMKDGG
                     HSAASIFRRLKEQCYAPPEYRVFPNNPLPIDALQQDLDVEYPALIKGYVRAGAYICGE
                     PHWDTDFNSADVLVMMPISRINARYARHFLK"
     gene            3135..3941
                     /locus_tag="PK856_06425"
     CDS             3135..3941
                     /locus_tag="PK856_06425"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRL02039.1"
                     /translation="MAEKRNTLLMGWRMARVGWHLVSGCTQIAVFFGRSSPEQKQRRI
                     QNWSAQLLAACGMKIKVVGDINSLPKATGRFWVSNHVSWLDIFAINAVQPMRFVGKDD
                     IQTWPVIGWLVARSDTIFISRSNRNAALAANDIISNALQQGANIAIFPEGTSTDGHQL
                     KPFKHSLFQAPISAQVPVQTLCVRYPMENGELNPAPAYHGDISIWQTLVSLLQQPQSL
                     VEVHILAGVETKDVSRQQLSALAQAQIAQCLEQYKIETVEDTQSYSPTVS"
BASE COUNT         1101 a          913 c         1060 g         1133 t
ORIGIN      
        1 gaggatttta aagtattgtg atgtgtgatt tgaatttaca ggccgcttaa aagcggcctg
       61 taatgcatta agcggcctgt aaatgttacg cgtgttgcat gatgtcgttg gcttgttgca
      121 agcgctctac cgtgcccacg tccagccaca agcctgtgtg gaggctgccc gtgacttgtt
      181 gatggttcat ggcctcgcgt aacaaaggcg cgagttttgc tgcttgacgc gcaggcgtgt
      241 gttggaacaa atcagggtgg tagattccgc aaccgctgaa ggtgtattgc tgcggcgcat
      301 ggctgaccac atgctcaccg tccaaaccaa aatcgccatt gggattgtgc tcaggattgt
      361 tcaccaacca caggtgtgcc aagctttgtt gcttggccat ttgcgcggct gcatgctcgg
      421 cttgggcaaa atcaatgtcg gtcaacacat cgccattgat gaccaaaaat ggctccttgc
      481 ccaacagcgg caaggcagtg gcgatgcctc ccgcagtttc caagccttgc gcaccctctg
      541 gggaatagac aatgtgcaaa ccgtaatcgc ggccgtcttg tagcacatct tccagttgct
      601 gacctaacca cgcatggtta atcacgactt cggtgaaacc cgcttgctgt aagcggcgca
      661 aatgccaacc gattaaaggc tcgcggccca cttttaataa aggtttcggt gtggcatcgg
      721 ttaaggggcg catgcgctca ccgcgacccg cggccaatat cattgctttc atggcgtttt
      781 caaggaaata aatgaaacaa gcagtgtaat ccaaatctcc aaaagccgac accgttgtga
      841 ttttgccatt ttgctgcaaa gcaacagatt ttgtgccatt aacaggaagc taaaattcga
      901 tttaagaaat aaattcttct gcattttatt ttataggaaa ttaaatttca ttaaagcatt
      961 taaatatagg gttgtttgga attatatttc aattaagtcg aataattgcg cttgattttt
     1021 ggtttcaaag cgtttagcct tgcttcatcc gaattttaat tgcttttttg agaaacagag
     1081 ttctgcatga gtatcgctat gaaaaatacc gacgacgtta aaatcaaaga aataaaagaa
     1141 ttattgcctc cgattgccca tttgtacgaa ttgccattga ccgagcctgc cagcactttg
     1201 gtacacgaca cgcgtgctga aatctctgat ttgattcatg gcaaagacca acgcttattg
     1261 gtggtggtcg gcccatgttc cattcacgat cccaaagccg ctttggaata tgctgaacgc
     1321 ttgatgccat tgaaaaagaa atacgaaaaa gaattggtga ttgtgatgcg tgtgtatttt
     1381 gaaaaaccac gcaccaccat tggttggaaa ggtttgatta acgatccgca tttggatggc
     1441 tcgttcgaca tcaattttgg cttgcgccaa gcccgtcatt tgttgttgga attgaacaat
     1501 atgggcatgc ctgcatcaac cgaattcttg gacatgatta caccacaata ctatgccgat
     1561 ttgatcagtt ggggcgcgat tggtgcgcgc accaccgaaa gccaagttca ccgtgaattg
     1621 gcgtctggct tgtcttgccc tgtgggcttt aaaaacggta ccgatggcaa catgaaaatc
     1681 gctattgatg ccatcggtgc ggccagcaac ccgcatcatt tcttgtctgt gaccaaaagc
     1741 ggccattctg cgattgtgcg caccgcgggc aatcccgatt gtcacgtgat tttgcgcggt
     1801 ggcagcaaaa acaccaatta tgacagcgag tcgattcaaa gcgcggttgc cgaattgagc
     1861 aaagcaggtg tgactcctaa attgatggtg gattgcagcc atgccaacag ccgcaagaaa
     1921 tacgaattgc aaatggaagt gggccgcgat gtggccgagc aaatcaaagt gggtgaaagc
     1981 aatttgatgg gcgtgatgat agaaagccat ttggtggaag gtcgccaaga cttggtagaa
     2041 gggcaagagt tgtgttacgg ccaaagcatt accgatgctt gcataggttg gggcgacact
     2101 gaagtcttgt tggctgaatt ggcggatgcg gtgaaaacgc gcattgctgc ttaaggttgc
     2161 agatgatgaa caattgaata aaaagcgtcc tgtaaggttt acaggacgct ttttatnnnn
     2221 nnnnnngtgt aaaaaaccta gattgcgcat ttggtggacg gttggtcata aaactgtaat
     2281 acatcaaatc atcagttttg tttgctgaca catgtttgtc attcacactt tgtagagtgt
     2341 gcagcatcgt gcttgaacat actcaatacg gagaaaagta atgttacaac tgcaaaccca
     2401 aagtcacgtt tacatccaag gtggaagttt aagcgtgtca cttgctcgca gtgaatttga
     2461 gatagaggaa gcacaacgct tacgctataa ggtattcgct gaggaaatgg gtgccaatat
     2521 tgtgtctgac aatggtttgg acaccgatga atatgatgac tactgtcagc atttattggt
     2581 gcgtgaacgt gaaaatgatc gcatcattgg atgttaccgt ttgttgacag ccgaaggcgc
     2641 agtggcaatc ggaaattggt attcggccaa cgaattcgat ttgagccgct tacaacacat
     2701 cttgccccag gcggtggagt tgggacgtgc ctgtgttcat caagattacc gatcaggcag
     2761 caccatcacc ttattgtggg caggcttgat gcaattcatg cagcaacaag gcttggaata
     2821 catgataggc tgcggcagca tgtgcatgaa agacggtggc cacagtgcgg ccagtatttt
     2881 ccgtcgactc aaagagcagt gctatgcccc acccgagtat cgcgtgtttc caaacaatcc
     2941 tttgcccatt gatgcgttac agcaggattt agacgtggaa taccctgcgt taatcaaagg
     3001 ttatgtgcgt gcaggggctt atatttgtgg cgagccacat tgggatacgg attttaatag
     3061 tgcggatgtg ttggtgatga tgccgatttc acgcatcaat gcccgttatg cccgtcattt
     3121 tttgaaataa tggcatggct gagaaacgca acacattgct aatgggctgg cgcatggcgc
     3181 gtgtgggttg gcacttggtg tcaggctgca cccaaattgc cgttttcttt ggacgcagta
     3241 gtccagagca aaaacagcgt cgcattcaaa attggtcggc tcaattgttg gcagcgtgtg
     3301 gcatgaaaat aaaagtggtg ggcgacatca atagcctgcc taaggctaca ggccgctttt
     3361 gggtgtccaa tcatgtttca tggctggata tttttgctat caatgccgta cagcccatgc
     3421 gttttgtggg taaggatgat atccagactt ggccagtcat tgggtggctg gtggcacgct
     3481 ccgataccat tttcatcagt cgcagcaatc gcaatgcagc cttggcggcc aacgacatca
     3541 ttagtaatgc gttgcaacaa ggggcaaaca ttgccatttt cccagagggc acatctacgg
     3601 atggccacca actcaaaccg tttaaacaca gtttgtttca agcccctatc agtgcccaag
     3661 ttccagtgca aaccttgtgt gtgcgttatc ctatggaaaa cggtgaatta aacccagctc
     3721 ctgcttacca cggcgacatc tccatctggc agaccttggt gagcctgctg caacagcctc
     3781 agtctctggt agaagtccac attttggcag gcgttgaaac caaggatgtc agccgtcaac
     3841 agctcagcgc tcttgcccaa gcccaaatag cacagtgcct agaacaatac aaaatagaaa
     3901 cagtggaaga tacacagtcg tattctccta ctgtgtctta attcacagta atgacgttat
     3961 agacacaaat aattaaagcg gtctgtaaag tttataggcc gcttctttgt atattggtaa
     4021 tgcacataat tactatagtg agtcttctgc gacagttttt taacagttgc ttatatatgt
     4081 atgaatgcga gtgttaagac tgcatgggcg actatgcatc gagtaaacag tcatcgcgcg
     4141 agcctgttgt ttggtgttta gggtgtttgt gagagatgct gcttaaaatt tcagctgatt
     4201 tgaggttttg ttaaaaa
//
LOCUS       DAOMVK010000165         4213 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_6050_length_4213_cov_2.643819, whole genome shotgun sequence.
ACCESSION   DAOMVK010000165 DAOMVK010000000
VERSION     DAOMVK010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6050_length_4213_cov_2.643819"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="PK856_06430"
     CDS             <1..578
                     /locus_tag="PK856_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRL02040.1"
                     /translation="AAVMNAVILTSILSAGNSGMYASTRMLYAMGKDGLAYKTFARTN
                     VSGNPIIALLLTSVVVLAIFGLELGNEQAYQYIIAASGLTGFIAWLGIALSHYRFRRA
                     YVAQGHDLKQLVYRAKWFPLGPILALVLCVLVILGQDTDLVLHGKVDWNAITITYMGV
                     PIFLAFYLYHKLRYKTRMIPLRDVDLSQDHH"
     gene            731..2458
                     /gene="argS"
                     /locus_tag="PK856_06435"
     CDS             731..2458
                     /gene="argS"
                     /locus_tag="PK856_06435"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRL02041.1"
                     /translation="MKLHQTVANVVEAAFLQAGLDNDAILLQPAGKPEFGDYQINGVM
                     GAAKRRKANPRDLASLVAAALQANTDVVATAEVAGPGFIKLRLNPAFLNQHLQAALAD
                     ARLGIEAAAAPQTIVIDYSSPNLAKELHVGHLRSTIIGDSLNRILSHLGHKVIAQNHV
                     GDWGTQFGMLVAYLVEQQKTGDADMALSDLEEFYRNAKVRFDEDEDFANAARDYVVKL
                     QSGDEKVLDLWRHFVASSMQHAEEVYAKLGVLLTPDDVIGESYYNNDLQPVVDDLMAR
                     GLAVESNGAKVVFLPEFKNKEGEDAAYIVQKAGGGFLYSTTDLATIRHSVVTFNADRL
                     LYVVDARQALHFQQLFTTARKAGYLPESVQAEHIAFGTMMGEDGKPFKTRDGGTVKLM
                     DLLKESEERATALVQSKNADLAQDAAAHIGHTVGIGAVKYADLAKSRTSDYIFNWSTM
                     LSFEGNTAPYLQYAYTRVQSVLRKAAEQHVVAGDIAITEAAEQQLALALLQFEDVLSH
                     VADSAYPHHLAAYLYQVATLFSRFYEQCPILKAEPAVMASRLSLATLTGNTLKQGLDL
                     LGIDVLEVM"
     gene            complement(2554..2937)
                     /locus_tag="PK856_06440"
     CDS             complement(2554..2937)
                     /locus_tag="PK856_06440"
                     /inference="COORDINATES: protein motif:HMM:NF018684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HRL02042.1"
                     /translation="MKRSTSILLAGTAMLMSSFAWAQYDPQCEDNAQYAQDSQMCYQA
                     REKHFDGVLNQSYQALMKQQSSTGKAKLRQMQRAWIRERDTECRAVVAKIAVSAGVAG
                     DKCFADFTEDRALEMRSWLKQGKRF"
     gene            3302..3793
                     /locus_tag="PK856_06445"
     CDS             3302..3793
                     /locus_tag="PK856_06445"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRL02043.1"
                     /translation="MNLNNCFFMCAVAFSLLMSSPALTLPKLSDLSLPQIDTAKVFQA
                     GSENMGMSYVGGREGMAHEYSRKFAEYQQAALAGKADAQYSLAVAYEYGMGVSQNNAL
                     ALDWYRKSAAQNYVRAQSRMGLAYAKGQLGLAANTHASAKWFDKASANQQNQSLDMIN
                     RMY"
     gene            3961..>4213
                     /locus_tag="PK856_06450"
     CDS             3961..>4213
                     /locus_tag="PK856_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959051.1"
                     /note="seems to be involved in biofilm formation; in
                     asymptomatic bacteriuria E. coli strains 83972 and VR50,
                     the ygiD gene is upregulated during biofilm formation in
                     urine; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HRL02044.1"
                     /translation="MQNLHNLYQHSRVLPDTDTMPVFFFGHGSPMNAIEENEFTQTWN
                     QIGANLPTPTAILAISAHWLTQGVTAVTVMGAPRTIHDFG"
BASE COUNT         1033 a          981 c         1083 g         1116 t
ORIGIN      
        1 ccgccgcggt gatgaatgcg gtgattttga cctcgatttt atcggcgggc aattcgggca
       61 tgtatgcgtc tacacgcatg ttgtatgcaa tgggcaaaga cggcttggct tacaagacgt
      121 ttgcacgcac caatgtgtcg ggcaatccca tcatcgcttt gttgctcacc agtgtggttg
      181 tgttggcgat atttggtttg gaattgggca atgagcaggc ttaccaatac atcattgccg
      241 cttcgggctt aacaggcttc attgcatggt taggcattgc cttgagccat tatcgttttc
      301 gccgtgctta tgtggcgcaa gggcacgatt tgaagcaact ggtttaccgc gccaaatggt
      361 tcccactggg cccgattttg gccttggtat tgtgcgtgtt ggtgattttg gggcaagaca
      421 ccgatttggt tttgcacggt aaagtcgatt ggaacgcgat taccattacc tatatgggcg
      481 tgccgatttt cttggcgttt tacctctacc ataaattgcg ttacaaaacc cgtatgattc
      541 ccttgagaga tgtggatttg agccaagacc accattaagc ggcctgtaaa ctcagaacca
      601 gcatgggtgt ttgcgtgctg attcaatatg agccagtaaa tatgttgcgg ccacattagt
      661 gtggccctgc cttttgcttt acaataactt ctttttgatg ggcatattgc ccgcagcgaa
      721 acagacagac atgaaactac accaaaccgt agcgaatgtg gtagaagcgg cttttttaca
      781 agcaggctta gacaacgacg cgattttatt gcaacccgca ggcaaacctg aatttggtga
      841 ttaccaaatc aatggcgtga tgggtgcggc caaacgccgc aaagccaatc cgcgcgactt
      901 ggcaagccta gtggctgcgg ctttgcaagc caacaccgat gtggtggcta ctgcagaagt
      961 ggcgggccca ggcttcatca agttgcgctt gaatccagcc ttcttgaatc agcatttaca
     1021 ggccgcattg gccgatgcgc gtttgggcat agaagctgcg gcagcgccac aaaccatcgt
     1081 gattgattat tcttctccca atttggccaa agaactgcac gtgggccatt tgcgctctac
     1141 catcatcggc gacagcttga accgcatttt gagccatttg ggccacaaag tgattgcgca
     1201 aaaccacgtt ggcgattggg gcacacagtt tggcatgttg gtggcttact tggttgagca
     1261 acaaaaaacc ggcgatgccg acatggcctt gtctgatttg gaagaatttt accgcaatgc
     1321 caaagtgcgt ttcgatgaag acgaagactt tgccaatgct gcgcgtgact acgtggtgaa
     1381 attgcaaagt ggcgacgaga aggtattgga cttgtggcgc cattttgtcg cttcgtctat
     1441 gcaacacgcg gaagaagtgt atgccaaatt gggcgtgttg ctcacgcctg acgatgtgat
     1501 cggtgaatcg tattacaaca acgatttgca acctgtggta gacgatttga tggcgcgcgg
     1561 tttggcggta gagtctaacg gtgccaaagt ggtgtttttg cctgagttca aaaacaaaga
     1621 aggcgaagac gcggcttaca tcgtgcaaaa agcaggcggt ggtttcttgt attccaccac
     1681 cgacttggcc accatccgcc attcggttgt gactttcaat gccgaccgct tgttatacgt
     1741 ggtagatgcc cgtcaagcct tgcatttcca acaattgttc accaccgcgc gcaaagcagg
     1801 ctatttgcct gaaagcgtgc aagccgaaca cattgctttt ggcaccatga tgggtgaaga
     1861 cggcaaaccg ttcaaaaccc gtgacggcgg cacagtcaaa ttgatggact tgttgaaaga
     1921 atctgaagag cgcgccaccg ctttggtgca atcgaaaaac gccgatttgg cccaagatgc
     1981 cgccgctcac attggtcaca ccgtgggcat aggcgcagtg aaatacgccg atttggccaa
     2041 atctcgcacc tctgactaca tcttcaactg gtctaccatg ttgtcgtttg aaggcaatac
     2101 cgcaccgtat ttgcaatacg cttacacccg tgtgcaaagc gtgttgcgca aggccgccga
     2161 gcaacacgtg gtggcaggcg acatcgccat cactgaagcc gctgaacagc aactggcctt
     2221 ggccttgttg cagtttgaag acgtgttgag ccacgtcgcc gacagcgctt acccacacca
     2281 tttagcagca tatttgtacc aagtggcgac tttattcagc cgcttctacg agcaatgccc
     2341 gattttgaaa gccgagccag ccgtgatggc atcgcgtttg tctttggcca ctttaacggg
     2401 taatacctta aaacaaggtt tggatttgct cggcattgat gtgttggaag tgatgtaatt
     2461 ggagtgtcag ccatcagata gggatggcta taaatactga taacaagcgg cctgtaaatg
     2521 tttacaggcc gcttgttttg atgctgcact gatttagaag cgctttcctt gcttcaacca
     2581 tgagcgcatc tccagcgcac ggtcttcggt aaaatcggca aaacatttgt cgcctgcaac
     2641 acctgcagat actgcaattt ttgccacaac agcacgacat tcagtgtctc gttcacgtat
     2701 ccatgcacgt tgcatttggc gcaatttggc tttgccagtg ctggattgtt gtttcattaa
     2761 ggcttgatag ctttgattca ataccccatc aaaatgcttt tcacgcgctt gatagcacat
     2821 ttggctgtct tgcgcatact gggcattgtc ttcacattgc gggtcgtatt gagcccaagc
     2881 gaatgaactc attaacatgg ccgtgccagc aagcagtatt gaggtagagc gtttcatggt
     2941 ctggtccgat tcaaaaaagt gacgcttgca ttgtaatgca aacactcaaa atgagcggat
     3001 gaaataccaa gggtatccaa cttgctactt tcggtagtat ttttaagcct caatcagatg
     3061 aggtcaacag cagatgtgtg cactgtttgt gcaaagtcta gaaacaaatg aatcaagcct
     3121 tggtatgagg cttgattcaa acaagtgttg gatatcggcc aaaatattcg ccaattcatg
     3181 gacttgggtg gattgtttaa gtcgattttt attataatca tgtttatgta aataaaaatg
     3241 aatcagccga tttttattaa gtatggcttg atttgtgttg ttaaaacttg atgagatggt
     3301 cgtgaattta aataattgtt tctttatgtg cgctgtggcg ttttccctgt tgatgtcgtc
     3361 gcctgcgttg accttgccca agttaagtga tttgagcttg ccgcaaattg acaccgctaa
     3421 agtgtttcaa gcgggcagtg aaaacatggg catgtcgtat gtgggcgggc gcgaaggcat
     3481 ggcgcatgaa tacagccgca aatttgctga gtaccagcaa gctgccctag cgggcaaagc
     3541 ggatgcacag tacagcttgg ctgtggctta tgagtatgga atgggcgtga gccaaaacaa
     3601 tgccttggcc ttggattggt accgcaagag tgccgcgcaa aattatgtgc gagcgcaaag
     3661 ccgcatgggt ttggcttatg ccaaagggca attgggcttg gctgcgaaca cccatgcttc
     3721 agccaaatgg tttgacaaag cctcagccaa ccagcaaaac caaagcttgg acatgattaa
     3781 tcgcatgtat tgattaggtg ttgtgaacaa gcggcctgtc atgttacagg ccgtttgttt
     3841 tttatcttgc catgcaaact gtttcttatg gtgaaagaaa ccctgttgcg tgtagggatg
     3901 tatttgcgcc cagtctgggc atacaatgca gacatctcat aacacactgc ggcatccacc
     3961 atgcaaaacc tacacaacct ctaccaacac agtcgtgtct tgcccgatac cgacaccatg
     4021 ccggtgtttt tctttggcca cggctctccg atgaatgcca tagaagaaaa tgaattcacc
     4081 caaacctgga accagatagg tgcaaaccta cccacgccga cagccatttt ggcgatttca
     4141 gcccattggt tgacccaagg ggtaacggcg gtaacggtga tgggtgcgcc gcgcacgatt
     4201 catgattttg gcg
//
LOCUS       DAOMVK010000166         4197 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_6078_length_4197_cov_3.918397, whole genome shotgun sequence.
ACCESSION   DAOMVK010000166 DAOMVK010000000
VERSION     DAOMVK010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6078_length_4197_cov_3.918397"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PK856_06455"
     CDS             <1..222
                     /locus_tag="PK856_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02045.1"
                     /translation="MHKLAAAHKKRIFEALHQRLPYISVLKRPAIDSVMTMGEGLIVT
                     QQVSGNAAAALNATRQLAHHWTEKLLDTP"
     gene            219..1511
                     /gene="hisD"
                     /locus_tag="PK856_06460"
     CDS             219..1511
                     /gene="hisD"
                     /locus_tag="PK856_06460"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRL02046.1"
                     /translation="MKQLNSAATDFWQQLKDLQAFETAQDPKVDQIVADICHDVQTRG
                     DAAVIEYTNRFDGMSATSMADLTLSQAELKAAFELLPENVQAALTTAARRIADYHQHQ
                     KQSSWQYTDADGTVLGQQITALDRVGIYVPGGKASYPSSVMMNAMPAHVAGVQEIIMV
                     VPTPKGERNDIVLAAAYVAGVTQIFTVGGAQAVAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGIVGIDMVAGPSEILVIADGSTPADWVAMDLFSQAEHDEIAQSILVSTSAT
                     YAAQVQEAMSQLIDTMPRKDIINASLQNRGAIVLVKDLDEACEVANYLAPEHLELSVA
                     NAQDWAAKIRHAGAIFMGAYTSESLGDYCAGPNHVLPTSRTARFASPLGVYDFQKRSS
                     LIQVSQQGAQKLGEIASILAHGEGLTAHARAAEFRLDK"
     gene            complement(1594..2268)
                     /locus_tag="PK856_06465"
     CDS             complement(1594..2268)
                     /locus_tag="PK856_06465"
                     /inference="COORDINATES: protein motif:HMM:NF018604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HRL02047.1"
                     /translation="MQWNLSKVLSFNAGYVDTASFLALHGLFAAHVTGNFVTLGAALI
                     SDNHAGAWTKLAALPMFCVVVVCTRLLHTRLQQVGHASYRVLLWCMLLLFALAAGISW
                     FHTPFAPTDSWISFAVGMLLVAAMAIQNALHRIHWLKESPTTVMTGSTTQLMLDVADW
                     LGSPQGKANEIKARFNRTLPNMLGFALGCAVAALVFHFMPKWVLVMPFFLMVWAYAWK
                     DRYAKT"
     gene            complement(2405..3028)
                     /gene="ycaC"
                     /locus_tag="PK856_06470"
     CDS             complement(2405..3028)
                     /gene="ycaC"
                     /locus_tag="PK856_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HRL02048.1"
                     /translation="MTKPYVRLNKDDAVVLLVDHQTGLLSLVRDIDPDKFKNNVLALA
                     DMATYFKLPTILTTSFEDGPNGPLVPELKAQFPDAPYIARPGQINAWDNEDFVKAIKA
                     TGRKQLIVAGVVTEVCVAFPVLSAIEEGFEVFVIADASGTFNAMTRDAAWARMEAAGA
                     QMMTWFGAACELHRDWRNDVEGLGTLFSNHIPDYRNLMTSYATLTSK"
     gene            complement(3370..3822)
                     /locus_tag="PK856_06475"
     CDS             complement(3370..3822)
                     /locus_tag="PK856_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02049.1"
                     /translation="MWVLSVLALCGYFYRVVLQRPFATVAKHFGIVMVMHLVLNGLAL
                     VLSQLDWTGMARPQSAPMGLKWALMLFTKLYMALLVVALINFAAAFAKKMVERVVSFH
                     QEHNAQNVHKQPIQGLIRHQKRIVIGYQAVFVLGGVMMLWAVWFRMLV"
     gene            complement(3846..>4197)
                     /locus_tag="PK856_06480"
     CDS             complement(3846..>4197)
                     /locus_tag="PK856_06480"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HRL02050.1"
                     /translation="VLLQGMVVAMAGVYEFVRVDTTVHPFHPEHTEHVVTRGVYAFSR
                     NPMYLGMLLCLVAVAVLHWRQPLNVVWCIGFYAYINRFQIQPEERILTQKFGEPFKQY
                     CLQVHRWLGHRAAR"
BASE COUNT         1137 a         1195 c          967 g          898 t
ORIGIN      
        1 atgcataaac ttgccgcggc gcacaaaaag cgtatatttg aagccttaca tcagcgtttg
       61 ccttacatca gcgtgttaaa acgccccgcc atcgacagcg tgatgacaat gggtgaaggc
      121 ttgattgtga cccaacaagt gagcggcaat gccgcagcgg cgctcaacgc cacccgccaa
      181 ttggcacacc attggactga aaaactactg gacacaccat gaaacaattg aattctgctg
      241 ctaccgattt ttggcaacag ctcaaagatt tgcaagcgtt tgaaaccgcg caagacccaa
      301 aagtggatca aattgtggcc gatatctgcc atgatgtaca aacccgtggc gacgcagcgg
      361 tgattgaata caccaaccgt tttgatggca tgagtgccac ttccatggcc gatttgacct
      421 taagccaagc cgaattgaaa gccgcgtttg agctcctgcc agaaaacgta caggccgctt
      481 taaccaccgc cgcgcgccgc atagcagact accaccagca tcaaaagcaa tcctcttggc
      541 aatacaccga tgccgatggc acggtattgg gccaacaaat caccgcgctc gaccgcgtgg
      601 gcatttatgt gcctggtggc aaagcctctt acccaagctc ggtgatgatg aatgccatgc
      661 ctgcccacgt ggcgggcgtg caagaaatca tcatggtcgt gcccacaccg aaaggcgaac
      721 gcaacgacat cgttttggca gccgcttatg tggcaggcgt gacccaaatt ttcaccgttg
      781 gcggcgcaca agccgtggcg gctttggctt acggcaccga aaccatccct caagtggata
      841 aaatcacggg cccaggcaac gcgtttgttg ccgccgccaa gcgccgagtg tttggcatcg
      901 tcggcataga catggtggcg ggcccgagcg agattttggt gattgccgat ggctctaccc
      961 ctgccgattg ggtggcgatg gatttgttca gccaagccga acacgacgaa atcgcccaat
     1021 ccattttggt ttccacttcc gccacttacg ccgcccaagt gcaagaggcg atgagccaat
     1081 tgatagacac catgccgcgc aaagacatca tcaacgcgtc tttgcaaaat cgcggcgcga
     1141 ttgtattggt aaaagacttg gacgaagcct gcgaagtcgc caactacctc gcccccgaac
     1201 acttggaatt gtcggtagca aacgcgcaag attgggcagc gaaaatccgc cacgcaggcg
     1261 caattttcat gggcgcttac accagcgaaa gcttgggcga ctattgcgca gggccgaacc
     1321 acgtattacc caccagccgc accgcccgct tcgcctcgcc actcggcgtg tacgatttcc
     1381 aaaaacgctc cagtctgata caggtatcgc aacaaggcgc gcaaaaattg ggcgaaatcg
     1441 ccagcatctt ggcccacggt gaaggcctca ctgcccacgc ccgcgcagca gagttccgtt
     1501 tggacaaata aacattaatc gtttttgaaa cacaaacaag cggcctgtaa gcatttacag
     1561 gccgctttgc agatgtacag gctttatcat gcttcatgtc tttgcgtagc ggtctttcca
     1621 tgcataagcc cacaccatca agaaaaaagg catgaccagc acccatttgg gcatgaaatg
     1681 aaacaccaat gcggccactg cgcagcccaa ggcaaaaccc aacatattcg gtaaggtgcg
     1741 gttaaaacgc gctttgattt cgtttgcttt gccttgtggc gaacccagcc aatcggccac
     1801 atccaacatc aattgtgtgg tagaacccgt catcaccgta gtgggcgatt ctttcaacca
     1861 atgaatgcgg tgtaaggcat tttgtatcgc catcgccgcc accaacaaca tgcccacggc
     1921 aaaggaaatc cagctgtctg tgggcgcgaa cggggtgtgg aaccaagaaa tgcccgccgc
     1981 caaggcaaac agcaataaca tgcaccacaa caacacccga taagaagcgt gccccacttg
     2041 ttgcaaacgt gtgtgcaata agcgggtaca taccaccacc acgcaaaaca tcggcaaagc
     2101 cgccaatttt gtccatgcac ccgcatggtt atcgctgatt aaggccgcgc ccaaggtgac
     2161 aaaattgccc gtcacatggg cggcaaataa gccgtgcaag gccaaaaaac tggcggtatc
     2221 gacatagccc gcgttaaagc tcaacacctt gcttaaattc cactgcattt tgctttcctc
     2281 ctgctctcac accacccaac tgcaagctca gaaaagacgg ctctgagctt tggtttggtt
     2341 tcgatacaaa gcggcctgta aaatcgttta caggccgctt attacttgat gcgtatgctt
     2401 gatgttattt agacgtcaac gtggcgtatg aagtcatcaa attgcggtaa tcgggaatgt
     2461 ggttagagaa caaagtgccc aagccttcca catcgttgcg ccagtcgcgg tgcaattcac
     2521 acgcagcgcc aaaccaagtc atcatttgcg cgcctgccgc ttccatgcgc gcccaagcgg
     2581 catcgcgggt catggcattg aatgtacctg aagcgtcggc aatcacaaac acttcaaaac
     2641 cctcttcaat tgctgacaac acagggaagg ccacacacac ttcagtcacc acacccgcca
     2701 cgatcaattg tttgcggcct gttgctttga tggctttgac aaagtcttcg ttgtcccaag
     2761 cattgatttg gcctggacga gcgatgtaag gggcatctgg gaattgggct ttcaattctg
     2821 gcaccaacgg gccattgggg ccgtcttcaa aactggtggt caaaatggta ggcaatttga
     2881 aataagtggc catgtcggcc aaggccaaca cattgttttt gaatttgtca ggatctatgt
     2941 cacgcaccag tgacaacaaa cctgtttggt ggtctaccaa caacactacg gcatcatctt
     3001 tgttcaaacg aacgtaaggc ttggtcatat tgctctccta aaatcggcta aatgaatgtt
     3061 gcagcaacac ctatggcgat ggttccatgc cccacatcag gtgttgttga tgtgcaatca
     3121 tcttaagctc gatgatgggc cttgagtaga tggcaaactt caaacacatt gttctgtttt
     3181 tacagcaagt caaactgtac agactatttg ccacatacca tctggggaag atggacatcg
     3241 ggcctgctga aatggttcaa aatacccaaa aatcatcaga cttttgcacc caatgattat
     3301 catccagaca gttgcactct cagctgttgt cacaacacag tctgaatggc caccgtcaaa
     3361 tgctcaaatt tacaccaaca ttctgaacca caccgcccac aacatcatga ccccacccaa
     3421 cacaaacacc gcttggtagc caatgacaat gcgtttttga tgcctaatca agccttgtat
     3481 gggttgtttg tgcacatttt gcgcattgtg ctcttgatga aaactcacca ctcgctccac
     3541 cattttcttg gcaaacgcag ccgcgaaatt gattaaagcc accaccaaca aggccatgta
     3601 cagcttggta aacagcatga gtgcccattt caagcccatc ggagccgact gcggcctcgc
     3661 catacccgtc caatccagct gacttaacac caaagccaaa ccatttaaca ccagatgcat
     3721 caccatcacg atgccaaaat gcttggccac agtggcaaaa ggccgctgca ataccactcg
     3781 gtaaaaatag ccgcacaaag ccaacacaga aagcacccac atcagtgtgt tcaataacaa
     3841 tgccatcatc gtgccgcgcg gtgtcccaac caacggtgta cctgtaagca atactgcttg
     3901 aacggctcac caaatttttg cgtcagaatc cgttcttcag gctgaatttg aaagcggttg
     3961 atgtaagcat aaaaaccgat gcaccaaacc acattcaaag gctggcgcca gtgcaacacc
     4021 gcaaccgcca ccaaacacag caacatgccc aaatacatgg gattgcggct gaaggcgtac
     4081 acgccacgtg tgaccacgtg ctcggtatgc tcagggtgaa acggatgcac ggtggtgtcc
     4141 acacgcacaa attcatacac acccgccatc gccaccacca ttccctgcaa caacacc
//
LOCUS       DAOMVK010000167         4195 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_6082_length_4195_cov_3.534541, whole genome shotgun sequence.
ACCESSION   DAOMVK010000167 DAOMVK010000000
VERSION     DAOMVK010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6082_length_4195_cov_3.534541"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PK856_06485"
     CDS             <1..467
                     /locus_tag="PK856_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HRL02051.1"
                     /translation="NNLEITPAEAQEFINRYFARYPAVAEYMQAAKDRAHEQGYVETI
                     MGRRLYLPDIANKNGNIRAGAERAAINAPMQGTASDLIKRAMILVADWLQADGLSTRL
                     LMQVHDELVLEVPEAEIAVVSEKLPLLMAQAGNLKVPLVAEVGIGLNWDEAH"
     gene            complement(464..1051)
                     /locus_tag="PK856_06490"
     CDS             complement(464..1051)
                     /locus_tag="PK856_06490"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRL02052.1"
                     /translation="MWELILALALLLMALVWMGHRRYRALQGQNQAIWLQVERQLNRR
                     YDWVPMWVQTAKSILKKEMTALKTVIEARQAATRALKNIKQQPEYNHAMQEWLRAEAH
                     FTQCLRDLRALVQTTPELYNHPDLMPLRADLLRIEQDLYAACTQYNQTVDFYNQYKRC
                     FPKNLLAHALGHTQDALLLPIEDTIAFEHNFTEQA"
     gene            1459..2979
                     /locus_tag="PK856_06495"
     CDS             1459..2979
                     /locus_tag="PK856_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRL02053.1"
                     /translation="MKYPVPNSSSSPIQFKSQYENYIGGKWTPPVKGQYFDDICPITG
                     KAFTKVARSTAEDIELALDAAHAAKAAWNNSSSTERSNILLKIADRMEANLENIAVVE
                     TWENGKPVRETLNADIPLAIDHLRYFAGAVRAQEGGISEIDHDTVAYHFHEPLGVVGQ
                     IIPWNFPLLMAIWKLAPALAAGNCVVLKPAEQTPVSILYLMELVGDLLPAGVLNIVNG
                     FGVEAGKPLASNKRIAKIAFTGETTTGRLIMQYATENIIPVTLELGGKSPNIFFEDVM
                     AADDEYLDKAIEGFVMFALNQGEVCTCPSRALVQESIYDKFMEKVLKRVAAIKAGDPL
                     DTDTMIGAQASQDQLEKILSYLDIGKQEGAEVLAGGHQRKLDGDLADGYYVEPTVFKG
                     HNKMRIFQEEIFGPVVAVTTFKDAEEALEIANNTLYGLGAGVWTRNGTLAYRMGRAIQ
                     AGRVWTNCYHAYPAHAAFGGYKQSGIGRENHKMMLDHYQQTKCLLVSYSGKALGFF"
     gene            3036..3413
                     /locus_tag="PK856_06500"
     CDS             3036..3413
                     /locus_tag="PK856_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HRL02054.1"
                     /translation="MSHSRLDATPAALDLIAKLQAQHGDLMFHQSGGCCDGSAPMCFP
                     AGEFKVGAADVLLGHIADCPFYMSAAQFEYWQHTHLTLDVTPGRGASFSLEIPEGVRF
                     IIRSRLFTDVELQDLQAVRTGAL"
     gene            complement(3519..>4195)
                     /locus_tag="PK856_06505"
     CDS             complement(3519..>4195)
                     /locus_tag="PK856_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02055.1"
                     /translation="LSSVLGLGLGLASFSAMAFDDSVNLSVDGKSLVIQEVSGRVLGN
                     AEGTQAVLVDDIFAGQGKKAVPGYQVMFMGRTASVAVEAKKTADGKFVDDRMIHRGVK
                     AAVGIPVVNGKMDLANAKALNVALISDENREFKDGEKFRPRGEQLVKKDAKISNVKLS
                     VKQLNLPDVKETSGGGIVYTLSFDVNGRKVVSEGNSTFRDWNIAKAAMGERLFKVDER
                     VMKAQK"
BASE COUNT         1063 a         1106 c          990 g         1036 t
ORIGIN      
        1 ccaacaattt ggaaatcacc ccagccgaag cgcaagaatt catcaaccgc tatttcgccc
       61 gctaccctgc ggtagctgaa tacatgcagg cggccaaaga ccgcgcccat gagcaaggct
      121 atgtcgaaac catcatgggc cgccgcttgt acttgcccga tattgccaac aaaaacggca
      181 acatccgcgc aggtgcagag cgcgccgcca tcaatgcacc gatgcaaggc acggcgtctg
      241 atttgattaa acgcgcgatg attttggtgg cagactggtt acaggccgat ggtttaagca
      301 cccgtttatt aatgcaagtg cacgatgaat tggtattgga agtgccagaa gcagaaatcg
      361 ccgtggtcag tgaaaaattg cctttattga tggcgcaggc aggcaatttg aaagtgccgt
      421 tggtggccga agtcggcatc ggtttgaact gggacgaggc gcactaagcc tgttctgtga
      481 agttgtgctc aaatgcgatg gtgtcctcaa tgggcaagag caacgcatct tgggtatgac
      541 ccaatgcatg ggccaataga ttcttaggaa aacagcgctt gtattggttg taaaaatcaa
      601 ccgtttggtt gtattgcgta caggccgcat acaggtcttg ctcaatgcgc aacaaatcgg
      661 cacgcaaagg cattaaatca ggatggttgt ataactcagg cgtggtttgc accaaagccc
      721 gcaaatcgcg caaacattgg gtgaaatgag cctcagcacg caaccattct tgcatcgcat
      781 ggttgtactc cggttgctgt ttgatgtttt tcaaggcccg tgtggccgct tggcgtgctt
      841 ctatgacagt ttttaaagcc gtcatttctt ttttcaaaat gcttttagcc gtttgtaccc
      901 acatcggtac ccaatcgtag cggcggttta actgtcgctc cacttgcagc caaatggctt
      961 ggttttgacc ttgcaatgct cggtagcgac ggtgtcccat ccaaaccaag gccatcaata
     1021 gcagcgccaa ggccagtatt aactcccaca tgttgccacc accccttctt taaactcata
     1081 cacctattgg tatgcatgcc ttggttttgc ggcatgtgtt ctggccactt agcaaaaaat
     1141 gtaaaaccta tgggtctaac aagctcacct actcaagtag acccatgaaa gcatggaagt
     1201 ttcccaacat taatcagcca tctgcctgac agtttactcc ataacattcc cttatcgcag
     1261 tataaatttt gcataatttt ccattcttta actccaaatc tgatttatac ccacacatca
     1321 cttggtatat gcccatcgca tttaagttca attccgcact gcaaaaagac ccagtgtatt
     1381 caatgtgcta aggtgaggct catggcagta aaaccagtca tcacccatcc gagtaagcac
     1441 attctgagga gtcacggcat gaaatatcct gttcccaaca gttctagtag tccgattcaa
     1501 ttcaaaagcc aatatgaaaa ttacatcggt ggcaaatgga cgccaccagt gaaaggccaa
     1561 tatttcgatg acatttgccc gattacaggc aaagccttca ccaaagtggc gcgctctact
     1621 gccgaagaca ttgaattggc actggatgcc gcccatgccg ccaaagccgc ttggaacaat
     1681 tcatccagca ccgagcgcag caatattttg ctgaaaatcg ccgaccgcat ggaggccaat
     1741 ttggaaaaca ttgcggtggt ggaaacgtgg gaaaacggca aacccgtgcg cgaaacctta
     1801 aatgccgaca ttcccttggc gatagaccat ttgcgctatt tcgcaggtgc ggtgcgtgcg
     1861 caagaaggcg gtatttcaga aatcgatcac gacaccgtcg cctaccattt tcatgagcct
     1921 ttgggcgtgg tggggcaaat cattccgtgg aatttccctt tgttgatggc gatatggaag
     1981 ctcgcacctg ccttggccgc aggcaattgc gtggtgttga aacctgccga acaaacgcct
     2041 gtatcgattt tgtatttgat ggaattggtc ggtgatttgc tgcctgcggg cgtgcttaac
     2101 attgtgaatg gttttggtgt agaagcaggc aagcctttgg ccagcaacaa acgcatcgcc
     2161 aaaattgcgt tcacgggtga aaccaccact ggccgcctca tcatgcaata cgctaccgaa
     2221 aacatcattc cagtcacctt agaattgggc ggcaaatcgc ccaatatctt ctttgaagac
     2281 gtgatggcgg ctgacgatga gtatttggac aaagccatcg aaggctttgt gatgtttgcc
     2341 ctaaaccaag gcgaagtgtg cacctgccca tcgcgggcac tggtgcaaga gtcgatttac
     2401 gacaagttca tggaaaaagt gctcaagcgc gtggcggcca tcaaagccgg cgacccactc
     2461 gacaccgaca ccatgatagg cgcgcaagcc agccaagacc aattggaaaa aatcctctcc
     2521 tacctcgata tcggcaaaca agaaggcgca gaagtgttgg caggcgggca tcagcgcaaa
     2581 ctcgatggcg atttggcaga tggctattat gtagagccca ccgtgttcaa aggccataac
     2641 aaaatgcgca tctttcaaga ggagattttt ggcccagtgg tggcagtgac caccttcaaa
     2701 gacgccgaag aagccttaga aatcgccaac aacaccttgt atggcttggg tgcaggcgtg
     2761 tggacgcgca acggcacgct cgcctaccgc atgggccgcg ccattcaagc aggtcgcgtg
     2821 tggaccaatt gctatcatgc ctaccctgcc cacgccgctt ttggtggcta caagcaatcg
     2881 ggcataggcc gtgaaaacca caaaatgatg ctcgaccact accaacaaac caaatgcttg
     2941 ctggtgagct attccggcaa agccttgggc tttttctaaa cccacaagcg gcctgtaact
     3001 atttacaggc cgcttttttc ttctaggagc atcacatgag tcactcgcgt ttggatgcca
     3061 cacctgccgc tttggattta atcgccaaac tccaagccca gcacggggat ttgatgtttc
     3121 accaatcggg tgggtgttgt gatggcagcg cgcccatgtg ttttcctgcg ggtgaattca
     3181 aagtgggcgc agctgatgta ttgctcggcc acatcgccga ctgcccgttt tacatgagtg
     3241 cggcgcaatt tgaatattgg caacacactc accttacttt agacgtcacc cctggtcgcg
     3301 gcgcgagttt ttctttggaa attcccgaag gtgtgcgctt catcatccgc tcacgtttgt
     3361 ttacagatgt ggagttgcaa gatttgcaag ccgtacgcac tggggcttta taagcttcac
     3421 acaacacatc cagcacttgt ttgctgtact tcatccatac aaaagcggcc tgtaaatcgt
     3481 ttacaggccg cttttaaaca ctctaagatg cttacgtctt acttttgagc tttcatcaca
     3541 cgctcatcca ctttaaataa acgctcgccc atcgcggctt tggcgatatt ccaatcgcgg
     3601 aaggttgagt taccttcaga cacgactttg cggccgttca catcaaaact caaggtgtaa
     3661 acaatgccac cacctgaagt ttctttcaca tctggcaagt tcaattgttt cacgctcaat
     3721 ttcacattgc tgattttggc gtcttttttc accaattgct caccgcgtgg gcgaaatttt
     3781 tcgccgtctt tgaattcgcg gttttcatcg gaaatcaagg ccacgttcaa ggctttggca
     3841 ttcgccaaat ccattttgcc gttgactacg ggaatgccaa cagcggcttt cacgccgcgg
     3901 tgtatcatgc ggtcgtcgac gaatttgcca tcagcagttt tcttggcttc aaccgccaca
     3961 gaagccgtgc ggcccatgaa catcacttgg tagccaggca cggctttttt gccttgaccc
     4021 gcaaaaatgt cgtctaccaa gacggcttgt gtgccttcag catttcccaa cacacggcct
     4081 gaaacctctt gtatcaccaa agatttgcca tccacagaca aattcacgct gtcatcaaaa
     4141 gccatcgcgc tgaacgatgc caaacccaaa cccaagccca aaacgctgct caaca
//
LOCUS       DAOMVK010000168         4193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_6085_length_4193_cov_4.236829, whole genome shotgun sequence.
ACCESSION   DAOMVK010000168 DAOMVK010000000
VERSION     DAOMVK010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6085_length_4193_cov_4.236829"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="PK856_06510"
     CDS             <1..570
                     /locus_tag="PK856_06510"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRL02056.1"
                     /translation="YRALYPTPDMIAHISQDFFQERGCVPWFPNAVIHDEDLAAQMRV
                     LFDVLEQPSNALFKETLYVATVAKLISRHGQKPQAINTLPDAAHKALLLKDHIAAHPE
                     QEHKLSDLAALVDLSPWHCLRQFKKFVGMPPHAWLIQVRLQRARQYLKQGMPIPQVAF
                     DCGFSDQSHLNRHFKRALGVTPAQYIVSL"
     gene            713..1432
                     /locus_tag="PK856_06515"
     CDS             713..1432
                     /locus_tag="PK856_06515"
                     /inference="COORDINATES: protein motif:HMM:NF015549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HRL02057.1"
                     /translation="MSSHTEPLQAASTPTPKHTLFWQGMMAMMPLSIAVIPWGLLAGS
                     MAMNTGLSVAQAIAMSAVIFAGAAQLVSLGLVMAGASFATIVVTVFFLTAQHFIYALT
                     LRGDIAKHKLLTRVGLGFLLTDELFALAARPEQRQVPYLFGAGLCFYLFWVVASIAGI
                     VLASMVPNLQQYHLDFSIVAIFIPIIVILLKNRAAIAGLVVTTVCVLMMKHLQLEGAM
                     IVSGMAGMLVAALIERKKEAA"
     gene            1429..1746
                     /locus_tag="PK856_06520"
     CDS             1429..1746
                     /locus_tag="PK856_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HRL02058.1"
                     /translation="MTWALLLTLAAVVFFNRYVFLEPKVAIRLPKLLERMLHYAAPCL
                     LAAICVPIVFYDDTGAWRGVTQNAYVYAGVVCCVLAWFTRRVLLSMALSLVVFYTLMY
                     GVF"
     gene            1962..2789
                     /locus_tag="PK856_06525"
     CDS             1962..2789
                     /locus_tag="PK856_06525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRL02059.1"
                     /translation="MPQTSKIDVIIPCYNAALTLERAVMSVVLQTHLQHIIIVDDGSC
                     DHTVATAHRLQAAYPNKIRVECLPQNGGVARARNWGMLVSNAEIVAFLDADDAYQAQV
                     LAFSHAVMQFRPDLPALRLGLIPVDLDAKYSAHPQFDYGWRHVEMTVGGNMVFRRAFL
                     LACGGFPQDALFRRLGGEDVALGLAVLASSQMGTAFDDVGLDEIAVLHHCRPDMYAYK
                     LLDGVLFGLGRSDITEADQQDAQAVTDGIVARLQQLQQWLHTEHKGKVPLKVELAPK"
     gene            2850..>4193
                     /gene="purB"
                     /locus_tag="PK856_06530"
     CDS             2850..>4193
                     /gene="purB"
                     /locus_tag="PK856_06530"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRL02060.1"
                     /translation="MDSIYALSPLDGRYAKSVEALRPIFSEYGLMKFRVQVELSWLKA
                     LAAEEKIVEVPAFSEVTIKEIDDVIANFSLADAAVVKTIEATTNHDVKAIEYWLKERF
                     SGVPEVLAVSEFIHFACTSEDINNLSHALMLKTARDEVVLPALQDIINKLTEMAHEFA
                     AMPMMARTHGQPATPTTLGKELANVVFRLQRQVESLKAQEFLGKINGAVGNYNAHMVA
                     YPDVDWEKHCGLFVKSLGITFNPYTIQIEPHDYIAEFFQTLTRVNTILIDFNRDVWGY
                     ISLHYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLSFMAEKLPVSRWQRD
                     LTDSTVLRNMGVGLGYTILGWSAHLRGLNKLETNPATLLADLDATWELLAEPIQTVMR
                     RYAVANPYEQLKDLTRGKDGITPETLKIFIEKLDIPAFEKEKLLALRPETYIGKAV"
BASE COUNT          958 a         1089 c         1093 g         1053 t
ORIGIN      
        1 tatcgcgccc tctaccccac gcccgacatg attgcccaca tcagccaaga tttttttcaa
       61 gagcgcggct gtgtgccttg gtttccgaat gcggtgatac acgatgaaga tttggccgca
      121 caaatgcgcg tgttgtttga tgtgttggag cagccgagca atgccttgtt caaagaaacc
      181 ctgtatgtcg ccaccgtggc caaactcatc agccgtcatg ggcaaaagcc gcaagccatc
      241 aacaccttgc ccgatgccgc gcacaaagcc ttgctgttaa aagaccacat cgccgcccac
      301 cccgagcaag aacacaagct cagcgatttg gccgccttgg tagacttaag cccgtggcat
      361 tgcctgcgcc aattcaaaaa attcgttggc atgccgccgc atgcttggct gatacaagtg
      421 cgtttgcaac gagcgcggca gtatttaaaa caaggcatgc ccatcccgca agtggctttt
      481 gactgcggct tttccgacca aagccacctc aatcgccatt tcaaacgcgc actgggcgtg
      541 acccctgcgc aatacattgt gtcgttgtaa acgtgcttcg gtgctgcaaa ttctgatata
      601 aaagattgtg tccatcaaat cagctcagtg atgtccacgc gcaattttgt acaagacaaa
      661 ccctaagtca caacgacata ctgacttttt ttttacctaa cgaaagccac cgatgagcag
      721 ccacaccgag cctttacagg ccgcatcgac acccacgccc aaacatacct tgttttggca
      781 agggatgatg gcgatgatgc ctttgtccat cgccgtgata ccgtggggct tgcttgcagg
      841 ctctatggcc atgaacacgg gcttgagcgt agcgcaagcc attgccatgt cggcggtgat
      901 ttttgctggc gcggcacaat tggtgagcct aggtctggtg atggcgggcg catcgtttgc
      961 caccatcgtg gtgacggtgt tctttctcac tgcccaacac tttatttacg cgctgacgct
     1021 gcgcggcgac attgccaaac acaagctgct cacgcgcgtg ggactgggct ttttgctcac
     1081 cgatgaattg tttgccttgg cagcgcgacc tgagcagcgc caagtgccgt atttgtttgg
     1141 cgcgggcctg tgtttttatt tgttttgggt ggtggcgagc attgcgggca tcgtgttggc
     1201 gagcatggtg cccaacttgc agcaatacca tttggacttc tccatcgtcg ccattttcat
     1261 tcccatcatc gtgattttgc tgaaaaaccg cgccgccatc gcaggcttgg tggtgaccac
     1321 ggtgtgcgtg ttgatgatga aacatttgca acttgagggc gcgatgattg tgtctggcat
     1381 ggcgggcatg ttggtggcgg ccttgattga gcgtaaaaag gaggcagcat gacttgggct
     1441 ttgttactga ctttggcggc ggtggtgttt ttcaaccgct atgtgttttt ggagccgaag
     1501 gtggcgatac gcttgcccaa gctgctggag cgcatgttgc actatgccgc accgtgtttg
     1561 ctggcggcta tttgcgtgcc catcgtgttt tacgatgaca ccggcgcgtg gcgcggcgtg
     1621 acgcaaaatg cctatgtgta tgcaggcgtg gtgtgttgcg tgttggcgtg gtttacccgc
     1681 cgcgtgttgc tcagcatggc tttgagtttg gtggtgtttt acaccttgat gtatggtgta
     1741 ttttgagttt tttgaagaca aataagcggc ctgtaaactg ttttcaatgg gtttacaggc
     1801 tgcttgtatt cacaagctcg tgccaacatc gcattccatc tcagtcacac cccctctgca
     1861 ccaagtctct aaaaacttgt ccgctcaacc cacaaatctg cgttaaaata acggcttatt
     1921 tgcatccttc ccacaccaaa accgcgctga ctgccacatc tatgccacaa actagcaaaa
     1981 tcgacgtcat cattccctgt tataatgccg ccttgacttt ggaacgtgcc gtcatgagcg
     2041 tggtgttgca aacgcatttg cagcacatca tcatcgttga cgatggctca tgcgaccaca
     2101 ccgttgccac agcgcatcgt ttacaggccg cttatcccaa caaaattcgc gtggaatgtt
     2161 tgccgcaaaa tggcggcgtg gcacgggcgc gcaattgggg catgttggtc agcaacgctg
     2221 aaatcgtggc ttttttggat gccgacgatg cgtatcaagc gcaggtattg gcgttcagcc
     2281 atgcggtaat gcagtttcgc cccgatttgc ccgctttgcg tttgggctta atccctgtgg
     2341 acttagacgc gaaatacagc gcccacccac aatttgatta cggctggcgg catgtggaaa
     2401 tgacggtagg tggcaatatg gtgttcaggc gcgcattttt gctcgcttgc ggcggttttc
     2461 cacaagacgc gttgtttcgt cgcttgggtg gcgaagacgt cgccttgggc ttggccgtat
     2521 tggccagtag ccaaatgggg acggcatttg acgacgtggg cttggatgag attgcggtgt
     2581 tacaccactg ccgcccagac atgtatgcct acaaattgct cgatggcgtg ttgtttggtc
     2641 taggccgcag cgacatcacc gaggcagacc aacaagacgc gcaagcggtg accgatggca
     2701 tcgttgcgcg cttacagcaa ttgcaacagt ggttgcacac cgaacacaag ggcaaagtgc
     2761 cgcttaaagt ggaacttgcc ccgaaatgat tttttgccag tgcttacagg acgcctgtag
     2821 cttattttgt aaacgaaaga cacggacaaa tggacagtat ttacgctctt agccctctcg
     2881 atggacgtta cgccaaatca gttgaggctt tgcgcccgat tttctctgaa tatggcttga
     2941 tgaaattccg cgtacaagtg gaattgtctt ggctcaaagc cttggcagcc gaagaaaaaa
     3001 ttgttgaagt gcccgcattc agtgaagtca ccatcaaaga aatcgacgac gtgattgcca
     3061 atttctcttt ggccgacgcc gccgtggtga aaaccattga agcgaccacc aaccacgacg
     3121 tgaaagcgat tgaatactgg ttgaaagagc gtttcagcgg tgtgcctgag gttttggcag
     3181 tgagcgaatt catccacttt gcctgcacca gcgaagacat caacaacttg agccatgctt
     3241 tgatgctcaa aaccgcgcgt gacgaagtgg tgttgcctgc tttgcaagac atcatcaaca
     3301 aactcaccga aatggcgcac gaatttgccg cgatgccgat gatggctcgc acccacggcc
     3361 aacctgcgac tcctactacc ttgggcaaag aattggccaa cgtggtgttc cgtttgcagc
     3421 gccaagttga aagcctaaaa gcgcaagaat tcttgggcaa aatcaatggc gccgtcggca
     3481 actacaatgc ccacatggtg gcctaccctg atgtggattg ggagaaacac tgtggcttgt
     3541 ttgtgaagtc tttgggcatc accttcaacc cttacaccat ccaaatcgag ccacacgatt
     3601 acatcgcaga attcttccaa actttgaccc gcgtgaacac gattttgatc gacttcaatc
     3661 gcgacgtttg gggctacatc tctttgcatt acttcaagca aaaagtgaaa gctggtgaag
     3721 taggctcttc taccatgccg cacaaagtga atccaattga ctttgaaaat tctgaaggca
     3781 acttgggcat ggccaatgcc gtattgagct tcatggctga gaaattgcct gtgtcacgct
     3841 ggcagcgcga cttaaccgac tctaccgtct tgcgcaacat gggcgtgggc ttgggttaca
     3901 ccattttggg ctggtctgca cacttgcgcg gcctgaacaa attggaaacc aacccagcga
     3961 ctttgttggc cgatttggac gccacttggg aattgttggc agagccaatt caaaccgtga
     4021 tgcgccgcta cgccgtggcc aatccttacg agcaattgaa agatttgact cgcggcaaag
     4081 acggcatcac cccagaaacc ttgaaaatct tcatcgaaaa attggacatt cctgctttcg
     4141 agaaagaaaa attattggct ttgcgtcctg aaacctacat cggcaaagcc gtt
//
LOCUS       DAOMVK010000169         4184 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_6100_length_4184_cov_3.144587, whole genome shotgun sequence.
ACCESSION   DAOMVK010000169 DAOMVK010000000
VERSION     DAOMVK010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6100_length_4184_cov_3.144587"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /gene="argF"
                     /locus_tag="PK856_06535"
     CDS             <1..783
                     /gene="argF"
                     /locus_tag="PK856_06535"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRL02061.1"
                     /translation="STTYLGPSGSQIGHKESIKDTARVLGRMYDAIEYRGFGQDIVEE
                     LAEYAGVPVYNGLTDEWHPTQMLADLLTMSEHTDKPLQQIAYAYTGDARCNTGNSLLE
                     LGCLMGMDVRIAAPKSLQPSVEVIAHCQALAAKSGARMLITDDLQAAVEGVDFIHTDV
                     WVSMGEPESAWAERIALLKDYQVNSSVMAASGNAQVKFMHCLPAFHDTDTKMGRWVEE
                     TFGIVGLEVTDEVFESAASIVFDQAENRMHTIKAVMVATLAK"
     gene            909..1127
                     /locus_tag="PK856_06540"
     CDS             909..1127
                     /locus_tag="PK856_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02062.1"
                     /translation="MNKKYTGFASPFDPTQVDKAEALARAKKLQKRLLYSKIFFPYII
                     VMALVGVCDTYFHVTVSGVTWAWLGGFL"
     gene            complement(1491..2561)
                     /gene="lptG"
                     /locus_tag="PK856_06545"
     CDS             complement(1491..2561)
                     /gene="lptG"
                     /locus_tag="PK856_06545"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HRL02063.1"
                     /translation="MSLLTRYLLKRLGAMTVFSLIALLALYVFFDVLQESGDLGKDGY
                     GVGSMLAYVALLIPAHIYELLPLAVLIGGLIALSGLANNSELTVMKASGVSTRRFVKT
                     LLGFGLVFAILTAVLGEWVAPKSQQYAERLKNHVQNSNGPVGQSGVWIKNDGRYINFG
                     ELLPDLSVRNLTVYSHNAEYELSQALHADSAHNIGGNQWQLKQVAITDLSAKATKTST
                     QAQATLTLEVDDAVLRALQVQPEQMSLLALGDYIEHLKANHQQSERYEIAWWRKLTYP
                     IAALAMALIALAFTPQATRRTNTTIKLFIGIFVGVGFHFANRFFAFSAQLYNIAPSIA
                     AILPTLLFLIGAIYWIRRQERR"
     gene            complement(2558..3664)
                     /gene="lptF"
                     /locus_tag="PK856_06550"
     CDS             complement(2558..3664)
                     /gene="lptF"
                     /locus_tag="PK856_06550"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HRL02064.1"
                     /translation="MIYQRKFIKELSFTAVGIFVVLLAILLCTQVVNLLGRAADGRVA
                     IDAVGTLIGFWMIGLTPLLLILTAFISLLTVLTRYWRDSEMAVWRAGGLSLNRWLRPV
                     AWFVVPFSLLIAVLTLAVLPWAESRSQEYAQILKQQQALSMVEQGVFRSVGRDRVYFI
                     ERFDSEQGIAKNLFIREQLPEGKSAIITAQSGNFKLDDPKERTLTLLNGRRYVGTPGQ
                     ADYEVLNFDKLDLLVQNNIDPIKPSDARQAIPTSTLWGSAEPRHQSELMWRLSLPISI
                     LILGLLAVPLSYFNPRAGHSYNLILAVVLFLIYQNGLTLLRRAVEDGLPFWLGLLPMH
                     VLMLLLGWLLLRYRNQPSGAFWPTLKAALKGNRS"
     gene            3770..>4184
                     /locus_tag="PK856_06555"
     CDS             3770..>4184
                     /locus_tag="PK856_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRL02065.1"
                     /translation="MKFSIISEKNQLEQNAAHVWVGIKGEKQTPAVAANLLAALQNGL
                     DVEADVDSACGLDVNAGQLNAVAVLQVVAGASRAKLEKHALKIAQYVVEQKAQAVSID
                     VCAVDAATQALLVDVLVLAFSQAVYRFDEHKRDAKA"
BASE COUNT         1083 a          978 c         1131 g          992 t
ORIGIN      
        1 agcaccacgt atttgggccc atcgggcagc caaattggcc acaaagaaag catcaaagac
       61 accgctcgcg tgttgggccg catgtacgat gcgattgaat accgtggctt tggtcaagac
      121 atcgtcgaag aattggccga atacgcaggc gtgcctgttt acaatggcct gactgacgaa
      181 tggcatccta cccaaatgct ggccgacttg ctcaccatga gtgagcacac agacaagcct
      241 ttgcagcaaa ttgcctatgc ctacaccggc gacgcacgct gtaatacggg caattccttg
      301 ttggaactgg gttgtttgat gggcatggac gtgcgcattg ccgcgccaaa atccttgcaa
      361 ccgagcgttg aagtcattgc ccattgccaa gccttagcag ccaaaagcgg cgcgcgcatg
      421 ttgattaccg atgatttaca ggccgctgta gaaggcgtgg acttcatcca caccgatgtg
      481 tgggtgtcta tgggcgagcc tgagtcggct tgggccgagc gcattgcctt gctcaaagac
      541 taccaagtga acagcagcgt gatggcggcc agtggcaatg cccaagtcaa attcatgcat
      601 tgcttgcccg ctttccatga caccgacacc aaaatgggcc gttgggtaga ggaaaccttt
      661 ggaatcgtgg gcttggaagt caccgatgag gtgtttgaaa gcgccgcttc catcgtgttc
      721 gaccaggcag aaaaccgtat gcacacgatt aaagcggtga tggtggccac tttggccaag
      781 tagagtgttt gaagcattta ataagcggcc tgtaagtgta caggccgctt tgttttggcc
      841 agagtatggg tgtgcaacat gcttcactga gcacagagaa tgtgaatgca ggatacaggg
      901 aatgatagat gaacaaaaaa tacacaggct ttgctagccc atttgaccct actcaagttg
      961 ataaagctga ggcattggct agagccaaaa aacttcaaaa gcgcctgctg tactccaaga
     1021 tatttttccc atacatcatt gtgatggcgc tggtaggtgt ttgtgataca tattttcatg
     1081 tgacggtatc gggtgtcaca tgggcttggt tagggggatt tttgtagtgt tgggcattta
     1141 cagcattttt tcaaccttag tattgggcat ttgttcatat tgccaacagt ttcaaaaact
     1201 caatggccaa acactgggtg tggatgcaca gtcgatttct tattccaaag gggtgtcacc
     1261 attcatacac aggtgtagcc gttgtgctgc gccgttgtca gttaaagccg tgaaccatga
     1321 ttatgccgat taaaaaaaga ttgtgtgtct gagggtaatg tttgaggcaa ggaaatgtta
     1381 aagcacaaga tgagtgcttt tattgtatct attagctgtt gaattaagaa tcaataagcg
     1441 gcctgtaaat atttacaggc cgcttttatc attcaaatcc ctttattggc ttatctgcgt
     1501 tcttgtctgc gtatccaata aatcgcgccg attaagaaca acaacgtggg caagatggcg
     1561 gcgatggagg gcgcaatgtt gtagagctgc gcactgaagg cgaagaagcg gttggcaaag
     1621 tggaaaccca cgcccacaaa aataccaatg aacaatttga tggtggtgtt ggtgcggcgc
     1681 gtggcttgcg gggtgaaggc cagcgcaatc aaggccatgg ccaaggcggc gatggggtag
     1741 gtgagcttgc gccaccatgc gatttcatag cgctcagact gttggtggtt ggccttgagg
     1801 tgttcgatgt agtcccccaa agcgagtaaa gacatttgct caggctgcac ttgcaaagcg
     1861 cgcaagaccg catcgtccac ttccaaagtc aatgtggctt gggcttgggt actggttttg
     1921 gtggctttgg cgctcaagtc ggtgatggcg acttgtttga gttgccattg gttgccgccg
     1981 atgttgtgcg ccgaatcggc atgtaaggct tgtgacaatt cgtattcggc attgtggctg
     2041 tagaccgtga ggttgcgcac gctcaaatca ggtagcaatt caccaaaatt gatgtaacgg
     2101 ccatcgtttt taatccacac gccactttgc cctactgggc cattgctatt ttgcacgtgg
     2161 tttttcaagc gttcggcgta ttgttgcgat ttgggcgcaa cccattcgcc caagactgcc
     2221 gtcaagatgg caaatacaag gccaaagccg agcaaggttt tgacaaagcg gcgggtagac
     2281 acgccactgg ctttcatcac cgtcaattcg ctgttgttgg cgaggccaga caaggcgatt
     2341 aagccgccta tcagcacggc caagggcagc aattcgtaaa tgtgggcggg tatgagcaag
     2401 gcaacataag cgagcatgct gcccacacca taaccatctt tgcccaagtc gccgctttct
     2461 tgtaacacat cgaaaaacac atacagggcg agtaaggcaa tcaaactgaa taccgtcatg
     2521 gcacccaagc gtttgagcag gtagcgggtt aataagctca tgaacggttt cctttcaacg
     2581 cggctttaag tgtcggccaa aaggcaccac tgggttgatt gcggtagcgc aataacaacc
     2641 agcccagcaa cagcatcaac acatgcatag gcaacaggcc taaccaaaat ggcaaaccgt
     2701 cttctactgc acggcgcagc aaggtcaaac cattttggta aatcaaaaac aacaccacag
     2761 ccaaaatcag attgtagctg tggcctgcac gcggattgaa ataagacaaa ggtacggcaa
     2821 gcagacccaa aatcaaaatt gatataggca aagacaagcg ccacatcagc tcagattggt
     2881 ggcgtggctc agcgctgccc cacaaagtgc tggtggggat ggcttgcctt gcatcactgg
     2941 gcttgatggg gtcgatgttg ttttgcacca ataagtccaa tttatcgaaa ttcagcacct
     3001 cgtaatcggc ttgacctggt gtgcccacat agcggcggcc attcaataag gtgagagtgc
     3061 gctctttcgg gtcgtccaat ttaaaattgc ccgattgtgc ggtgatgatg gcacttttgc
     3121 cttcgggcaa ttgctcgcga atgaacaggt ttttggctat gccttgctcg ctgtcgaagc
     3181 gctcaataaa gtacacgcgg tcgcggccca cgctgcgaaa caccccttgt tcgaccatgg
     3241 acaaggcttg ctgttgcttg agaatttgcg cgtattcttg gctacggctt tcggcccatg
     3301 gcaatactgc caaggtcaac accgcaatca acaggctaaa cggcaccaca aaccaagcaa
     3361 ctggacgcag ccaacggttt aaggataagc cacccgcgcg ccacaccgcc atttcgctgt
     3421 cgcgccaata gcgggtgagc acggtgagca agctgatgaa ggctgtgagt atcagcaaca
     3481 aaggcgttaa gcctatcatc caaaaaccga ttaaagtgcc cactgcatcg atggcgacac
     3541 ggccatcggc tgcgcgccct agcaagttca ccacttgcgt acacagcaaa attgctaaca
     3601 acaccacgaa aattccgacg gcggtgaaag acagttcttt gataaatttg cgttgataaa
     3661 tcatataagc ttttgcctag ctggcatggt ttcaggaggc cgcgtcaggt acaatcagtg
     3721 tgtttgacgt tttgggcatt caattcgcca caatctggag ataaatgaca tgaaatttag
     3781 cataattagc gaaaaaaatc agctggaaca gaatgctgcc catgtttggg taggcatcaa
     3841 aggcgaaaaa caaacacctg ctgtggctgc taacttattg gccgcattgc aaaatggttt
     3901 ggacgtggaa gccgatgtag acagcgcgtg tggtttggat gtgaatgcgg ggcaattgaa
     3961 tgctgtggcg gtattgcaag tggtagcggg ggcgagccgt gccaagctgg aaaaacacgc
     4021 tttgaaaata gcgcaatacg tggtggaaca aaaagcccaa gcagtgagca tagatgtgtg
     4081 cgcagttgat gcggccaccc aagccttgtt ggtggatgtg ttggtgttag cgttttcgca
     4141 agcggtgtac cgctttgatg agcacaagcg cgatgccaaa gccg
//
LOCUS       DAOMVK010000170         4171 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_6136_length_4171_cov_3.900389, whole genome shotgun sequence.
ACCESSION   DAOMVK010000170 DAOMVK010000000
VERSION     DAOMVK010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6136_length_4171_cov_3.900389"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PK856_06560"
     CDS             <1..415
                     /locus_tag="PK856_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009116987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HRL02066.1"
                     /translation="GVKERKHRLMGFGHRVYKNMDPRAKIMKQTCDEVLNELGLHDDP
                     KFKLAMALEKIALEDPYFVERKLYPNVDFYSGIVLSALGIPVSMFTVIFALARTVGWI
                     SHWHEMIADPLYKIGRPRQLYTGATRRDYVPEAQR"
     gene            503..3337
                     /locus_tag="PK856_06565"
     CDS             503..3337
                     /locus_tag="PK856_06565"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HRL02067.1"
                     /translation="MMNEEIGLSYLYGSNAPYIEELYEMYLKDPSSVDSHWKQYFDEI
                     VTLPGAAARDVAHAPIQQSFVDMAKRPVTQHAASVALDANAMKKQVAVLRLVSAYRVL
                     GARHANIDPLQRMGSQYYSELDPATYGLTAEDMAVQFSVSNTFSSNDRLPLSEIINKL
                     QQTYCGTIGVEYMHITRSEEKHWVQDRFEKDLSTPHFNVETKKRILGKITAAETLERY
                     LHTKYVGQKRFSLEGGESTIPALDYLIQNSSAQGVEEVVIGMAHRGRLNVLVNILGKS
                     PKDLFSEFEGKYTVQLPSGDVKYHNGFSSDIPTNNGPVHISLAFNPSHLEIVNPVVEG
                     SVRARQRRRGDEGQKQVMPVLIHGDSAFIGLGVNQGTFNLSQTRGYSTGGTVHIVINN
                     QIGFTTSDTRDTRSTLYCTDIAKMIDAPIFHVNGDDPEAVCYVMQAAIDYRMQFKKDV
                     VIDLVCYRKLGHNEGDDPYLTQPMMYKKISQHPGSRAIYAEKLAKEGVVPAEGSEELI
                     REYRDALDKGEQVEQTKLTDYKRKHALDWSIYMGTHWAHPTETALPAADIQRLTEKFT
                     AVPADFHLHNTVKKLLENRKKMGAGEMPIDWGMAETLAYASLVTNGNGVRISGEDSGR
                     GTFSHRHAVLHDQNREKWDAGAYVPLRNMSDNQANFMVIDSILNEEAVLAYEYGFACS
                     APDQLVIWEGQFGDFANGAQVAIDQFIASGETKWGRLCGLTMILPHGYDGQGPEHSSA
                     RLERWLQLCSEHNMQVVMLSEASQMFHVLRRQVLRPYRKPLVIFMSKRLLRFKDSMSD
                     LSQFTEGSGFRTVIGDVVERDDAKVKRIVLCAGQVYYDLAAGRKERGLEEEVAIVRVE
                     QLYPFPYEVLAAELSRYANATEIMWVQEEPKNQGAWYQIRHRIEAVSTDKQKVLFAGR
                     PASASPAVGYMSKHAAQLKALIDDTFNLEA"
     gene            3396..>4171
                     /locus_tag="PK856_06570"
     CDS             3396..>4171
                     /locus_tag="PK856_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRL02068.1"
                     /translation="MSIIEVTVPVLPESVSEATLMTWHKKVGEAVARDENIIDLETDK
                     VVLELPAPQAGVIVEIVEQDGATVVSGQLIAKIDTAAKAGDAAPAAEATQAAAAPAAE
                     AAPQSNAQAGVAMPAAAKLAAEKGVDVASIQGSGRDGRVLKEDVANAQAAPAAAPTAN
                     PTFSTVTVSPTPTVQIAGDRPEQRVPMSRLRQRVAERLLQSQSENAILTTFNEVNMQP
                     VMDLRNKYKDKFQKEHDVKLGFMSFFVKAAVAALKKFPVV"
BASE COUNT         1123 a          918 c         1041 g         1089 t
ORIGIN      
        1 aggcgtgaaa gagcgcaaac accgtttgat gggctttggt caccgtgtgt acaaaaacat
       61 ggacccacgt gccaaaatca tgaaacaaac atgtgatgaa gtattgaatg aattgggctt
      121 gcacgatgat cctaaattca aattggcgat ggcattagaa aaaatcgcct tagaagaccc
      181 atacttcgtt gagcgcaaat tgtaccctaa tgtagacttc tactctggta tcgtattgtc
      241 tgctttgggt attccagtat ccatgttcac cgtaatcttc gctttggctc gtaccgtagg
      301 ttggatcagt cattggcacg aaatgattgc tgacccattg tacaaaattg gccgtccacg
      361 ccaattgtac acaggcgcaa cccgtcgcga ttacgttcct gaagcacaac gctaacagcg
      421 tggtgacagc actggtttac aggccggtgc tgtcttataa atttatggtc tgaacaacag
      481 acagcaacaa aggtcttttg ccatgatgaa tgaagaaatc ggcttgtctt acttatatgg
      541 ctccaatgcg ccatatattg aagagctgta tgagatgtac ttgaaagatc ctagctcggt
      601 cgacagtcat tggaaacaat actttgatga aatcgtaacc ttaccaggag cggccgcacg
      661 agatgtggcg catgcgccga ttcaacaatc atttgtcgat atggccaagc gtcctgtaac
      721 ccaacatgca gccagcgtcg cacttgatgc caatgcaatg aagaaacaag tcgcagtatt
      781 acgcttggtt tctgcttacc gtgttttggg tgcccgtcac gccaacatcg atccattgca
      841 acgcatgggc tctcaatatt actcagaatt ggatccagcg acttatggtt taactgccga
      901 agacatggca gtgcaattca gtgtgtctaa cactttttca agcaacgacc gtttgccgtt
      961 gtctgaaatc atcaataaat tgcagcaaac ctattgcggc accattggtg ttgaatacat
     1021 gcacatcacg cgctcagaag aaaaacactg ggtgcaagat cgttttgaaa aagatttgtc
     1081 taccccgcat ttcaatgttg aaaccaaaaa acgcattttg ggaaaaatca cagcggcaga
     1141 gacattagaa cgttatttgc ataccaagta cgtgggccaa aaacgcttct cattggaagg
     1201 tggcgaatcg accattcctg ctttggatta cttgatacaa aactcaagtg cgcaaggggt
     1261 ggaagaagtg gttatcggca tggcccaccg aggtcgtttg aacgtgttgg tgaatatttt
     1321 gggcaagtcg cctaaagatt tgttcagcga gtttgaaggc aagtacactg tgcaattgcc
     1381 tagtggtgac gtgaaatacc acaatggttt cagctcagac attccaacga ataatggccc
     1441 tgttcacatt tcattggcat ttaacccatc gcatttggaa attgtgaacc cagtggttga
     1501 aggttcagtg cgtgcccgtc agcgccgtcg tggtgatgaa ggccaaaaac aagtgatgcc
     1561 agtattgata catggtgact cggcctttat tggcttgggt gtgaatcaag gtacgttcaa
     1621 cttgtcacaa acccgtggtt acagcacagg tggtactgtg cacattgtga tcaataacca
     1681 aatcggtttt accacttcag acacacgtga tacccgttcg actttgtatt gtactgacat
     1741 tgcaaaaatg attgatgcac cgattttcca tgtgaacggt gatgatccag aagcagtgtg
     1801 ctatgtgatg caggccgcta tcgattaccg catgcagttc aaaaaagacg tggtcatcga
     1861 tttggtgtgt taccgcaaat tgggtcacaa cgagggcgat gacccttatt tgactcagcc
     1921 gatgatgtac aaaaaaatct cgcaacaccc aggttctcgt gccatttacg ctgagaaatt
     1981 ggccaaagaa ggtgtggttc ctgccgaagg cagcgaagag ttaatcagag aataccgcga
     2041 tgctttggac aaaggtgaac aggttgagca aaccaaatta accgattaca aacgcaagca
     2101 tgcattggat tggtcgatat acatgggtac ccattgggca catcctaccg aaaccgcctt
     2161 gcctgcagct gatattcagc gtttgactga gaaattcact gcggtgcctg ctgatttcca
     2221 tttgcacaac actgtgaaaa aactgttgga aaaccgcaaa aaaatgggcg caggtgaaat
     2281 gcctattgat tggggtatgg cagaaacctt ggcatatgcc agcttggtga ctaatggcaa
     2341 tggtgtgcgc atttctggtg aggactctgg ccgcggtacg ttctcacacc gccatgctgt
     2401 tttgcatgac caaaatcgtg aaaaatggga tgcaggtgct tacgttccat tgcgtaacat
     2461 gtctgacaat caagccaatt tcatggtcat tgactctatc ttgaatgaag aggcagtgtt
     2521 ggcatatgag tacggctttg cttgctctgc gccagaccag ttggtgattt gggaaggtca
     2581 atttggtgac tttgccaatg gtgcacaagt ggccattgac caatttattg cctctggtga
     2641 aaccaagtgg ggccgtttgt gtggcttaac catgattctg ccgcatggct atgatggcca
     2701 gggtcctgag cattcttctg cccgtttgga gcgttggttg caattgtgct ctgagcacaa
     2761 catgcaagtg gtgatgttgt ctgaagcctc acaaatgttc catgtgctgc gtcgtcaagt
     2821 tttgcgacca taccgtaaac cactggttat tttcatgtct aaacgcttat tgcgttttaa
     2881 agactccatg agtgacttga gtcaattcac tgaaggttcg ggcttccgaa cggtgattgg
     2941 tgacgttgta gaacgtgatg atgccaaagt aaaacgcatt gttttgtgtg caggtcaggt
     3001 gtattacgat ttggctgccg gtcgcaaaga gcgtggattg gaagaggaag ttgccattgt
     3061 tcgcgtagag caattgtatc cattcccata cgaagtactg gctgcagaat taagtcgtta
     3121 tgccaatgcc actgaaatca tgtgggtaca ggaagaaccg aaaaaccaag gtgcatggta
     3181 tcaaatccgt caccgtattg aggcggtttc tactgataaa caaaaagtac tctttgccgg
     3241 tcgccctgca agcgcgtccc ctgcagtggg ttacatgagc aaacatgcgg cacaattaaa
     3301 agcattgatt gacgatacgt ttaatttgga agcataagat ttagtcacca agtttaaggc
     3361 cacttggtgg ccacacctga ttaggagaat gtagtatgtc tattattgaa gttaccgtac
     3421 cggtattgcc tgagagcgtt tcagaagcta ccttgatgac ttggcataaa aaagtgggcg
     3481 aagccgttgc ccgtgatgaa aacatcatcg atttggaaac cgataaagtg gtattggaat
     3541 tgcctgcgcc acaagcgggt gtgattgttg aaatcgttga acaagacggt gctacggttg
     3601 tgtcaggtca gttgattgcc aaaatcgaca ctgctgccaa agcaggcgat gcagcccccg
     3661 ctgctgaagc cactcaagca gctgcggcac ctgcagccga agctgcgcca caaagcaatg
     3721 cgcaagctgg cgtggccatg cctgctgccg ccaaattggc ggctgaaaaa ggtgtggatg
     3781 tggcttctat tcaaggttca ggccgcgatg gccgtgtatt gaaagaagat gttgccaacg
     3841 ctcaagcggc tcctgctgct gcgccaactg ccaacccgac tttctctact gtgacggtaa
     3901 gtcctacgcc taccgtacaa atcgcgggtg accgtcctga gcaacgtgtg cctatgtcac
     3961 gcttgcgtca acgtgttgct gagcgtttgt tgcaatcaca atctgaaaat gccatcttga
     4021 ccacgttcaa cgaagtgaac atgcaaccag tgatggactt gcgtaacaag tacaaagaca
     4081 aattccaaaa agaacacgat gtgaaattgg gctttatgtc tttctttgtt aaagctgcgg
     4141 ttgctgcatt gaaaaaattc ccagtggtga a
//
LOCUS       DAOMVK010000171         4125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_6219_length_4125_cov_3.455528, whole genome shotgun sequence.
ACCESSION   DAOMVK010000171 DAOMVK010000000
VERSION     DAOMVK010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6219_length_4125_cov_3.455528"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..1494
                     /locus_tag="PK856_06575"
     CDS             169..1494
                     /locus_tag="PK856_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HRL02069.1"
                     /translation="MKTVTSIRLRLAAVISLAVLLSACPTTKPIGGGTPTSGGPKSAL
                     PPTPPGQVYGTPGQAVRYTSASFNQVPEWSGQSFKNSLISFQRGCSKLTNKVQWQAVC
                     RQAQSTPMNDQAARLFFENLFTPWQLSDNGNNSGTVTGYYEPVLLGDTRQTAQARFPI
                     YGIPTDFVSVPLSDDNRVRKGQVKVAVTGANQGSINGNGAYTANLNAFPITERSRSIK
                     GRVVGNQFVPYYTRAEINAGALNGKAPILAYANDPVELFFLHIQGSGRLQTPTGQFVR
                     LGYADKNEYPFKSVATYMANKGYLPLAQTNMQGLKAWMRQNPSKLAEVLGQNPSYVFF
                     RTTTGAADEGPIGALGAPLTGEYSAAIDKHHVELGAPIFVATTHPLNNKPHNRLIMAQ
                     DTGSAIKGGVRVDYYWGYGDAAGEVAGKMKHPGQVWMLLPNGYTPRYQP"
     gene            complement(1563..2102)
                     /locus_tag="PK856_06580"
     CDS             complement(1563..2102)
                     /locus_tag="PK856_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HRL02070.1"
                     /translation="MFDHKIIITGPVGVGKSTAIRAYSTTTVVDTDVQATDETRQIKK
                     NTTVAMDYGTIILDDETKVHLYGTPGQERFDFMWEILRSGSRGLIILLNAQAEDPAQE
                     LRFYLHAFGEIITKVPVVVGVNRAVKDEQTRTMMLGLQQVVRELGVEADVLLVDVRKR
                     KDVNKLVKRILFSDKLFAV"
     gene            complement(2259..>2557)
                     /locus_tag="PK856_06585"
     CDS             complement(2259..>2557)
                     /locus_tag="PK856_06585"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958535.1"
                     /note="converts 2-oxoglutarate to glutamate; in
                     Escherichia coli this enzyme plays a role in glutamate
                     synthesis when the cell is under energy restriction; uses
                     NADPH; forms a homohexamer; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HRL02071.1"
                     /translation="STLDAVDAFVEAKILYAPGKASNAGGVATSGLEMSQNSIRLNWT
                     HGEVDERLHGIMKDIHGNCVRYGKQEDGFINYVNGANIAGFVKVADAMLAQGVY"
     assembly_gap    2558..2600
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2601..3609)
                     /gene="gdhA"
                     /locus_tag="PK856_06590"
     CDS             complement(<2601..3609)
                     /gene="gdhA"
                     /locus_tag="PK856_06590"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HRL02072.1"
                     /translation="MNEFLNQVAVRDPNQPEFLQAVKEVMGSLWPFIAKNPQYGDHGL
                     LERLVEPERAIQFRVAWVDDNNQVQVNRAYRVQFNSAIGPFKGGMRFHPSVNLSILKF
                     LGFEQVFKNALTTLPMGGGKGGSDFDPKGKSDGEVMRFCQALMVELYRHLGPNTDVPA
                     GDIGVGGREVGFMAGMMKKLSNDASCVFTGKGMAFGGSLMRPEATGYGNVYFAEEMLR
                     TRGMNLKGQTVSVSGSGNVAQYAAEKATDLGAKVVTLSDSNGMIYVKDGISEDMLLEI
                     MDLKNVKRGRLEDLANVAGIEYIAGKRPWAVPVDIAMPCATQNELTGEDAAVLIHNGV
                     KC"
     gene            complement(3887..>4125)
                     /locus_tag="PK856_06595"
     CDS             complement(3887..>4125)
                     /locus_tag="PK856_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRL02073.1"
                     /translation="SSQYVGGMFGFYFNDKIPQSFADVSDSDKEAFKRFFHGMLERGV
                     SLAPSAFEASFMSAAHTDALIDETIAKAQDCFTA"
BASE COUNT         1112 a         1042 c          874 g         1054 t
ORIGIN      
        1 aaaatggttt gaacgtggat tctaccgtgt ggtacaacta cgccgccgat gtaccaactt
       61 acgatggtgc gggtgttggt gtgaaagtat caatgccgtt caaataattc cacattcatt
      121 tccaatgcat acaaaggcag ccttggctgc ttttgtcata taaagcttat gaaaacagtg
      181 acttcaatcc gattacgcct tgccgctgtc atttctttgg cagttttact cagtgcttgc
      241 cccaccacca aacccattgg tggaggtaca ccaacatctg gagggcccaa atcggccctc
      301 ccccccacac ctcctgggca agtgtatgga acccctggtc aagcagtacg ctatacctca
      361 gcttcgttca atcaagtgcc cgaatggtca ggtcaatcgt ttaaaaacag tttgatttcg
      421 tttcaacgcg gttgtagtaa actcactaac aaagtgcaat ggcaggctgt gtgccgtcaa
      481 gcccaaagca cccctatgaa tgaccaagcc gcgcgcttgt tctttgaaaa cctgttcaca
      541 ccttggcaac tcagtgacaa tggtaataac agtggtacgg taactgggta ttatgaaccc
      601 gtattgctgg gtgacacgcg acaaaccgcg caagcgcgct ttccgattta tggcattccc
      661 actgattttg tcagtgtacc cttatcagat gacaaccgtg tgcgcaaagg gcaagtaaag
      721 gtcgcagtga caggtgctaa tcaaggtagt atcaatggca atggcgcgta caccgccaat
      781 ttgaatgcgt ttccgattac tgaacgcagt cgcagcatca aaggccgcgt ggtaggcaat
      841 caatttgtgc cgtattacac ccgtgccgaa atcaatgcgg gcgcactcaa tggcaaagca
      901 ccgatactgg cttatgccaa tgatcctgtg gaactgtttt tcttgcacat tcaaggttca
      961 ggccgcttgc aaacgcctac aggccaattt gtacgcttgg gctatgcaga taaaaacgaa
     1021 tacccgttta aatctgtggc cacttacatg gcgaacaaag gctacttgcc tttggcacaa
     1081 accaatatgc aaggcctcaa agcatggatg cgccaaaacc caagcaaatt ggctgaagta
     1141 ttgggacaaa atccaagcta tgtgttcttt cgcaccacca caggtgccgc ggatgaaggc
     1201 ccgattggcg cattgggcgc acctttaaca ggggaatatt cggccgccat cgacaaacac
     1261 catgtcgaac tgggtgcacc gatttttgtg gccaccaccc acccgctcaa taataagcct
     1321 cacaaccgcc tcatcatggc acaagacaca ggttctgcga ttaagggtgg tgtgcgtgtg
     1381 gactattatt ggggttatgg agatgccgct ggtgaagtgg caggaaaaat gaagcatcca
     1441 ggtcaggtat ggatgttatt gcccaatggc tatactcccc gttaccagcc ttaagtttca
     1501 ttcagctgaa taacaaataa gcggcctgta aaccaagccg cttatttgtt ttgtaatgca
     1561 atttatacag caaacaattt gtctgaaaac aaaattcgct tcaccaattt attcacatct
     1621 ttgcgtttgc gcacgtccac caacaataca tcagcctcca cacccaattc tctgaccact
     1681 tgctgaagtc caagcatcat ggtgcgggtt tgttcatctt taacagcgcg attaacaccc
     1741 acaaccacag gcactttggt gatgatttcg ccaaaagcat gcaggtaaaa ccgcaattct
     1801 tgtgcagggt cttctgcttg ggcattaagc agaataatca gccctcgact gccactgcgc
     1861 aaaatttccc acataaaatc gaaacgctct tgccctggtg tgccatacaa atgcactttg
     1921 gtctcatcat ccaaaatgat ggtgccgtaa tccatcgcaa cagtggtatt ttttttgatt
     1981 tggcgtgttt catcggtcgc ctgcacatcg gtatcaacca cagtcgttgt actgtaggcc
     2041 cgaatggcag tgcttttccc cacacccact ggtcctgtga tgatgatttt atggtcaaac
     2101 attcgtgttt tccccactga cggtaatgtg taacaccacg ttttatttag agttatgttt
     2161 taaaattgaa tctgagcatt atacgtcaag aaaataaaaa gcccagcact atgctgggct
     2221 tgcaataaag tatttaaatt aatgttgtac acaacacatt aatacacccc ttgagccagc
     2281 attgcgtcgg caactttgac aaaaccggca atattggcac cgttcacata attgatgaaa
     2341 ccatcttctt gtttgccata acgcacacaa ttgccatgaa tgtctttcat gatgccgtgc
     2401 aaacgctcgt ctacttcacc atgtgtccaa ttcaatcgta tgctgttttg cgacatttcc
     2461 aaaccagaag tggccacacc acccgcattt gaagccttac ctggtgcata caaaattttc
     2521 gcctctacaa atgcatctac agcatccaaa gtagaagnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn cgcatttcac accattgtga attaataccg cagcatcttc
     2641 gcccgtcaat tcattttggg tggcacaagg catggcgatg tcaacaggca cagcccatgg
     2701 gcgtttgccc gcgatgtatt caatgcctgc cacattggcc aaatcttcca agcggccgcg
     2761 tttgacgttc ttcaaatcca tgatttcaag caacatgtct tctgagatgc catctttgac
     2821 gtaaatcatg ccgttggaat cgcttaatgt caccactttt gcgcccaaat cggtggcttt
     2881 ttccgccgca tattgcgcca cattaccaga gccagacaca gacacggttt gacccttcag
     2941 gttcatgccg cgtgtacgca gcatttcttc agcaaaatac acattgccat aacccgtggc
     3001 ttcaggacgc atcaagctgc caccaaacgc catgcctttg cctgtgaaca cacagctggc
     3061 gtcgttgctg agtttcttca tcatgcccgc catgaagccc acttcgcggc cacccacacc
     3121 aatgtcaccc gcaggcacat cggtattcgg gcccaaatgg cggtacagct ccaccatcaa
     3181 agcctgacaa aaacgcatca cttcaccatc gcttttgccc ttagggtcga agtcagagcc
     3241 gcctttaccg ccgcccatag gcaaggtggt caaagcattt ttgaagactt gttcgaaacc
     3301 taagaatttg aggatggaaa gattgactga ggggtgaaaa cgcatgccgc ctttgaatgg
     3361 gccgatggct gaattgaatt gaacacggta agcgcgattg acttgaacct gattgttgtc
     3421 atccacccat gcgacacgga attgaatggc acgctctggt tccaccaaac gctctagcaa
     3481 accatggtcc ccgtattgtg gattcttagc gatgaatggc cacaaactgc ccatcacttc
     3541 tttaacggcc tgtaaaaatt caggttggtt agggtcccga acggcaacct ggttcaggaa
     3601 ctcgttcata ttgctgtatt tcaaaatgac atccttgtgt aacacgataa aaacagtagc
     3661 tgcttggttc agtggggcat caagcactta aaaatcggta aaaactcaaa tcgagttcga
     3721 ttctagcaca aaccaagacc atgttaagaa agaaataagc catatttgct actttaaagc
     3781 aattaataca gcattttaga atttagatat cgaacaatta cgccaaacaa aataaaatgc
     3841 caatattgtt tcaaacattg gcatctgtct ttatttactt aacaaactag gctgtaaaac
     3901 agtcttgggc tttggcgatg gtttcgtcaa tcaaagcatc ggtatgggct gctgacatga
     3961 atgaggcttc aaatgccgat ggtgccaagc tcacaccccg ctctaacatg ccatggaaaa
     4021 agcgtttgaa cgcttcttta tcgctgtctg acacatcggc aaagctttgc gggattttat
     4081 cgttgaaata aaagccgaac atgccaccca cgtattggct ggaga
//
LOCUS       DAOMVK010000172         4124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_6222_length_4124_cov_3.191202, whole genome shotgun sequence.
ACCESSION   DAOMVK010000172 DAOMVK010000000
VERSION     DAOMVK010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6222_length_4124_cov_3.191202"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(273..710)
                     /locus_tag="PK856_06600"
     CDS             complement(273..710)
                     /locus_tag="PK856_06600"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRL02074.1"
                     /translation="MSVAEILAKKKAALALKNWEEGQAFLAANAEKEGIVTLPSGLQY
                     EVLQVGTGDKPTLSDTVVCHYHGQNLAGEVFDSSVERGEPATFRINRLIQGYQIALPL
                     MGTGSKWRIFVPSELAYKDEHKSKQISANSTLVFEVALLGIVK"
     gene            complement(806..1660)
                     /locus_tag="PK856_06605"
     CDS             complement(806..1660)
                     /locus_tag="PK856_06605"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRL02075.1"
                     /translation="MNDAEKIQRVCEYINLHLDEPLNLTVLCQQVYVSKYHFHRLFAA
                     YTGVTLMDFVQMGRMKRASYQLAFKPEMNILDIALQAGFDSHEAFGRAFKRRFQQTPS
                     GFRKQANWPFWHEQLRATLPKQEIKMKVEIIHRNVEPIAYLRHIGSPKKVLDSAAQFI
                     AWRKATGLSPIQSSKTYGIPYSDPDQTPEDAFQFDIAGSVTQAVPENEFGVQNAELPA
                     GRYAVIHHEGSHDTMNDSIYQLFREWLPEQQEQAGDFPIFFQYLNFVHDVAEHELRTD
                     ILLLLKEQ"
     gene            1867..2016
                     /locus_tag="PK856_06610"
     CDS             1867..2016
                     /locus_tag="PK856_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02076.1"
                     /translation="MAQLHPVAIGSTLSNTLCAHTPPVSPPLNTVVSAVVNQVAVVVA
                     AVLVA"
     gene            2085..2939
                     /locus_tag="PK856_06615"
     CDS             2085..2939
                     /locus_tag="PK856_06615"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRL02077.1"
                     /translation="MSVIQKHAAGLSMLLFVLLWGSAAIFTRWGLNHSSVAILLIGRY
                     AVALLVLSLIALKTRQWLPDKGTRWQVLQAGFLLVGGYSIAYFEAMAHGITPGLLATL
                     LGTQPILTLLITERRFSPLRLAGLLLALTGLILIVGQGLSLAGMDVWAWVFSFIALAC
                     ITFGTLLQKKIKQAPQQVMPLQFLLTLLMCFVLLPQTTMPTDFSLGYWIPVLWLGVVI
                     SVVAQLLLYKLIQSGNVVNITSLFYLVPIVTAILDFLVWGHAMSLTEILGMAAIVLGI
                     VLVFKPSK"
     gene            complement(3041..3316)
                     /locus_tag="PK856_06620"
     CDS             complement(3041..3316)
                     /locus_tag="PK856_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02078.1"
                     /translation="MNKLIHAVVIAAVASFSAMSMAATVAPAQTAPAKVAPAKVAPVK
                     APAAKAEAKKPAAAHKVSQKSYAKHKTVKHHAAKHNNHKTHKTPAKS"
     gene            complement(3557..>4124)
                     /locus_tag="PK856_06625"
     CDS             complement(3557..>4124)
                     /locus_tag="PK856_06625"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HRL02079.1"
                     /translation="MSFHWGQERQYRQNATQQAIARYAIDRGFNAVLGHHPHVVQGIE
                     RYKQGVIVYSLANFVFGGNGNPSDKDSVAVQLRVDFAGDALVAQDLTALPLSVSGVSN
                     GNDYRPVWANTAVQERTQAKMQAASNVDVRLGWREDLTSQALLAHTLKPSAPSRWCDD
                     SVSVWAPNVLETAAVRLPRCTLTASNWG"
BASE COUNT          939 a         1004 c         1004 g         1177 t
ORIGIN      
        1 gtaaaattca gcatcttgtt ttaaaccatc aagataattt tgatgatttt gggcaccttc
       61 agccgttatt ttactaccat gaatggaacg tctaaattgt gttgtaaaga aaggtcttct
      121 agacattttt gtaccttaat aaaatataac aaattgtttc tacaggatga tgccacttcc
      181 ctaagcgtaa aaaactttcg acaatctaat actatgaatc atttatgaag caaatgaccc
      241 atttacaggc cgctatttat cttctcaaac cactatttca caatgcccaa caacgccact
      301 tcaaacacca aagtggaatt ggcgctgatt tgtttggatt tgtgttcgtc tttgtaagcg
      361 agttctgagg gtacgaatat gcgccattta ctgcctgtgc ccatcaaggg caaggcgatt
      421 tggtaacctt gaatcaggcg gttgatgcga aatgtggcgg gttcgccgcg ctcaactgag
      481 ctgtcaaaca cctcacctgc caagttttgg ccgtggtaat ggcacaccac ggtgtcgctg
      541 agcgtgggct tgtcgcctgt gcctacttgc aaaacctcgt attgcaaacc cgaaggcaag
      601 gtcacaatgc cttctttttc ggcattggca gccaagaagg cttgtccttc ttcccagttt
      661 ttcaaagcca aagcggcttt ctttttggcc aatatttcgg caacagacat ggcattaacc
      721 cgttcaaaaa accgtattat accaagcacg gccatgcaca catggaagaa gcggcctgta
      781 aaccttacag gccgcttcta agcaatcact gctcttttaa taacagcaaa atatcggtgc
      841 gcaattcatg ctcggccacg tcgtgcacaa aattgaggta ttggaagaag atgggaaaat
      901 cccccgcctg ctcttgctgc tcaggcaacc actcgcggaa caattggtaa atgctgtcgt
      961 tcatggtgtc atggctgccc tcgtggtgaa tgactgcgta gcggcctgcg ggcaattcgg
     1021 cattttgcac tccaaattcg ttttcaggca cggcctgcgt gacgctgccc gcaatgtcaa
     1081 actgaaacgc gtcttctggc gtttggtcgg gatccgaata cgggatgcca taggttttgc
     1141 tggattggat gggcgacaaa cccgtggctt tgcgccacgc gatgaattgt gccgcactgt
     1201 ccaatacttt tttgggtgaa ccgatgtggc gtaaatacgc aatcggttcc acatttcgat
     1261 gtatgatttc aaccttcatt ttgatttcct gtttgggtaa agtggcacgc agttgttcgt
     1321 gccaaaatgg ccagttggcc tgtttgcgaa agccagacgg cgtttgctga aagcggcgct
     1381 taaatgcacg cccaaacgct tcgtggctgt caaagcctgc ttgcaacgcg atgtcgagga
     1441 tgttcatctc gggtttgaaa gccaattgat acgatgcgcg cttcatgcgc cccatttgca
     1501 caaaatccat caacgttacc cctgtgtaag cggcaaacag acggtgaaaa tggtatttgg
     1561 acacatacac ctgttggcac aaaaccgtca ggtttaaggg ttcgtccaaa tgcaggttga
     1621 tgtattcaca cacccgctga attttttcgg catcgttcat ctggttttca ttccttgttg
     1681 catggcaagc agtatcgcaa acgctcccta gcctcgcttg attgaaattg cggttttgtc
     1741 tgtcggtaac agacaacggt ggcatggttt gctcaccaaa atcgtttgca tctgtttcaa
     1801 tccatgttag tatcaagccc agtttcgcta agaaatcttt ttatcacaac acttttggag
     1861 gctggtatgg cgcaattgca ccctgttgca atcggttcga ctttatcgaa cacgctgtgc
     1921 gcacacacgc ctcctgtgtc tcctcctctc aacacggttg tgtctgccgt ggtaaatcaa
     1981 gtggcagtgg tagtggctgc tgtacttgtt gcttaatcgc ttaacttcaa attcgattat
     2041 tgattgagat attgcacgaa ctgattcgtg catgaggaac catcatgtct gtgattcaaa
     2101 aacatgcggc tggcttgtcc atgctgttat tcgtcttatt gtggggcagt gccgccatct
     2161 tcacccgttg gggcttgaac cattcttctg ttgccatctt gctgataggt cgctatgccg
     2221 tggccttgct ggtattgagc cttatcgcct taaaaacccg ccaatggttg cccgacaaag
     2281 gcacacgttg gcaagtgtta caggccggct ttttgttggt gggcggctat tccatcgctt
     2341 attttgaagc catggcacac ggcatcaccc caggcttgct cgcaaccttg ctcggcaccc
     2401 aacccatttt gaccttactc atcaccgagc ggcgtttttc accactgcgc ttggcaggat
     2461 tgttgctggc tttaacgggc ttgattttga ttgtgggaca aggcctgagc cttgctggca
     2521 tggatgtgtg ggcgtgggtc ttttcattca tcgccctcgc ctgcatcact tttggtactt
     2581 tattgcaaaa gaaaatcaag caagcaccgc agcaagtgat gcctttgcag tttttgctga
     2641 ctttgttgat gtgttttgtg ttgttgccgc aaaccacgat gcctaccgat ttcagcttag
     2701 gctattggat acctgtgttg tggttgggcg tggtcatttc ggtggtggcg caattgctgc
     2761 tttacaaatt gatacaaagc ggcaatgtgg tcaacatcac cagcttgttt tatttggtgc
     2821 ccatcgtcac ggcgattttg gatttcttgg tgtggggaca tgcgatgtcg ctcaccgaaa
     2881 tcttgggcat ggcggcaatc gtgttgggca tcgtgttggt gttcaaaccc agtaaataaa
     2941 cgcagtgtat gaaaaagcgg cctgtaacat tacaggccgc tttgttgtga tgattagatg
     3001 ggaattttaa tggtttttta actgacttca gaatgcctcc ttatgatttg gctggtgttt
     3061 tgtgggtttt gtggttgttg tgtttggcag cgtggtgttt cacggttttg tgcttggcgt
     3121 agcttttttg gcttactttg tgcgctgcag caggtttttt ggcttcggct ttggcagcgg
     3181 gtgcttttac aggggctact ttggctggtg ccactttggc aggggcagtt tgagcaggag
     3241 caacggttgc agccatgctc atggcagaaa atgaggccac agcagcaatc accacagcat
     3301 gaatcaattt gttcatttta caactccttc aaatcattta tcggtttcgg tgagtgcatg
     3361 atgcgctcga atacttaggc gaaacttagc ccaaaatcaa gctgtgtaag cccatttccg
     3421 cttttaatgt acttaagtta aaacagattc aggcccttgt tttgtgaaga aaaacatttg
     3481 cagcaagctt aatcccagat gaattaaaag cggcctgtaa atgtttacag gccgcttgtt
     3541 ttcatatctt gcagatttat ccccaatttg acgcagtcaa tgtgcaacga ggcaaacgca
     3601 cggcagcggt ctccaacaca ttcggtgccc atacagacac actgtcatca caccaacgac
     3661 tcggcgcaga aggcttgagt gtgtgcgcca gcaaagcctg actggtcaaa tcttcgcgcc
     3721 aacccaagcg cacatccaca ttgctcgcgg cctgcatctt cgcttgtgtg cgttcttgta
     3781 ccgccgtatt cgcccacaca gggcggtaat cattaccgtt actcacgccc gataccgaca
     3841 gcggcaaggc agtgaggtct tgcgccacca aagcatcgcc cgcaaaatcc acgcgcaatt
     3901 gcaccgccac gctgtctttg tcggacgggt tgccattgcc gccaaacaca aaattggcga
     3961 gcgaatacac aatcaccccc tgcttatacc gctctatgcc ttgcaccaca tgcggatggt
     4021 ggcccaatac cgcattgaag ccgcggtcta tggcatagcg agcaatggct tgctgggtgg
     4081 cattttggcg gtattgccgc tcttgccccc agtgaaacga catg
//
LOCUS       DAOMVK010000173         4055 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_6384_length_4055_cov_2.367000, whole genome shotgun sequence.
ACCESSION   DAOMVK010000173 DAOMVK010000000
VERSION     DAOMVK010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6384_length_4055_cov_2.367000"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PK856_06630"
     CDS             complement(<1..261)
                     /locus_tag="PK856_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRL02080.1"
                     /translation="MNPIKAMEVFVAVVKHQGLTAAAAELNLSNVMVGKYIAALEAQV
                     ATELIQRTTRQIRVTEAGWLFYESAQTAINAVNEAYLQLEALH"
     gene            410..1381
                     /locus_tag="PK856_06635"
     CDS             410..1381
                     /locus_tag="PK856_06635"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HRL02081.1"
                     /translation="MNGPTPIQRLHRLEQALACEQRNIEVYIKHDDASSLGQGGNKLR
                     KLEYLLGDAMEKGADVIITTGGIQSNHARLTAAACARLGLRCELVLASVVDNSEYDYH
                     HNGNLVLNDLFGAHVHLANEQMPAAVLIEQLQQRLQAEGHKPYFIPTGGSVALGCVGY
                     AEAAFEIAAQEQVMGVTFDAIHVANGSSGTHAGLLAGFEILGQARHVQAHSVLHNQAS
                     TIETTQTLMQETLQLLGHESAAPIMSVNINPEQMGSAYGMVTDEMKRCLRLLANSEGL
                     LIDPVYSGKAFAGMVHDIETGVVADNSRVLFVMTGGTPALYAYHNLL"
     gene            complement(1581..2465)
                     /locus_tag="PK856_06640"
     CDS             complement(1581..2465)
                     /locus_tag="PK856_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02082.1"
                     /translation="MKPMLPFYLQPIGLITSAAVLAICMLIGKLPHAWLCALLWLLWV
                     CVLEQRSLPSPLGRTVCVGLGVLLVMAILNLWQPTLPLSWFWIVLPLFIWAFHYRQHQ
                     HLLLTVKPLPLNTDTPLPLRLEQAVRYPWGSLFYSVSTVILLTAAFLALSSMVRQFKF
                     SYLFLAFLLCGLAYLAWKAREYFWKRYQFFAQYGGEWLQISERGLSWRKFNSTAHDLL
                     PNHTDYVHIHIAWENISRLEILTDSEAGKYLRIHSQIALNFGLNPHCMDIYEQEVAYP
                     LQTLETYLLQFKQQTNSD"
     gene            complement(2624..3631)
                     /locus_tag="PK856_06645"
     CDS             complement(2624..3631)
                     /locus_tag="PK856_06645"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF533 domain-containing protein"
                     /protein_id="HRL02083.1"
                     /translation="MTLAVMAAFADGSKHSSERNELSAIASSLGLDNKVQLLQIDQAV
                     LRQQAVLEEIAADISDGGSKQLAYEIALGVCEADGSISEAEQAFLSRLAQVLQLDGAP
                     LLSATPSQAIANTELSVSNQQQFNQGLHEVALAGAAIAEAAPKSLVQIDSQLEETLNK
                     RILKAAIINGALELLPQTAASMAIIPLQMRLVYGIGKDFGFDLDQGHIKDLLATLGIG
                     MTSQYLEQAGIKLLGTVLGGLRSSLGGKLGKSLTGMVGKVGNQAISSGMSFATTYALG
                     HVAKEYYASGRQFSPKVLKDAYARLMQDGIGLQPQYQTEIQQQAKTLDLNKIMQMVKG
                     Q"
     gene            complement(3782..>4055)
                     /locus_tag="PK856_06650"
     CDS             complement(3782..>4055)
                     /locus_tag="PK856_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02084.1"
                     /translation="TYFGHPSHGSPPSATGAVLSWQAVHVLINHQGDLIPAYSPSKET
                     SSQDSAALVSMPLTPKTMRWQRCVVRLQTDAQFITAHISVQDCRPE"
BASE COUNT          976 a          910 c         1102 g         1067 t
ORIGIN      
        1 gtgtaaggct tccaattgca ggtaggcctc gttgaccgca ttgatggctg tttgggcact
       61 ttcgtaaaac aaccagcccg cttcggtcac ccgaatttgg cgggtggtgc gctgtatcaa
      121 ttcggttgca acttgcgctt cgagggcggc gatgtatttg cctaccatca cattcgacag
      181 gttcaattcg gcagcggcag cagtcaaacc ttggtgtttg accacggcca caaacacttc
      241 catggctttg atggggttca ttgtgaatat ttagttaata aagaatgaat attatggcta
      301 ttttaattca gaatggcttg atttaacatc gcatttttac accaaaacgg acattgtgat
      361 gaagcttaca ttacctgcga agtggtcaaa attccccaag gcaacattat tgaatgggcc
      421 cacgcccata cagcgtttgc accgtttgga gcaagcctta gcttgtgagc aacgtaatat
      481 tgaggtgtat atcaagcacg atgatgcatc tagcttgggg caagggggca acaagctgcg
      541 caagctggag tatttattgg gcgatgccat ggaaaagggt gccgatgtca tcatcaccac
      601 aggcggcata caatcaaacc atgcccgctt gaccgccgcc gcttgcgcac gcttgggttt
      661 gcgctgtgaa ttggtcttgg catcggtggt ggacaacagc gaatatgatt accaccacaa
      721 tggcaatttg gtcttaaacg atttgtttgg ggcgcatgtc caccttgcca acgagcaaat
      781 gcctgccgct gtattgatag agcaattgca acagcgctta caggccgaag gccataagcc
      841 ttatttcatt cccacaggtg gctcggtggc tttgggctgc gtgggttatg ctgaggcagc
      901 gtttgagatt gccgcgcaag agcaagtcat gggcgtgaca tttgatgcca tacatgtggc
      961 caacggcagc tcaggaacgc atgcaggctt gttggcagga tttgagattt tgggacaagc
     1021 gcgtcatgtg caagcgcact cggttttgca caaccaagcc tccaccatcg aaaccaccca
     1081 gactttgatg caagaaacct tgcaattgtt gggacatgaa tccgctgcac ccatcatgtc
     1141 ggtcaacatc aatcctgagc aaatgggcag tgcttacggc atggtcacag atgaaatgaa
     1201 acgctgtctg cgcctgctgg ccaacagcga aggcttgctc atagaccctg tgtattcggg
     1261 caaggcgttt gcaggcatgg tgcatgacat cgaaacaggc gtggtggccg ataacagccg
     1321 cgtgttgttt gtgatgacag gcggcacacc tgctttgtat gcgtatcaca atttgctgta
     1381 attgctttta aaacccagtt tggaatgaat aacaagcggc ctgtaagcct gaaataggtt
     1441 tacaggccgc atgctggttt atggctgtcg ggtttaaggc aattaagagt atttaagtat
     1501 gctgttgaat cagcttattc aaacaatgtc actgcttctg agctggcaag gtcgtcattc
     1561 agctcgggca atatattcag ttaatcgctg ttggtttgtt gtttgaattg tagtagatat
     1621 gtttctaggg tttgtaatgg gtaggccact tcttgttcgt aaatgtccat gcaatgaggg
     1681 ttcaagccaa aattgagtgc gatttggctg tggatgcgca ggtatttacc agcttctgag
     1741 tctgttaata tttccaagcg gctgatgttt tcccatgcaa tatgaatgtg cacatagtcg
     1801 gtatggttgg gtaataagtc gtgggctgtg ctgttgaact tgcgccaaga caaaccccgt
     1861 tcgctgattt gtagccattc gccgccgtat tgggcaaaaa attgataccg cttccaaaaa
     1921 tactcgcgcg ctttccatgc caaatacgcc aaaccacaca agaggaaagc caaaaacagg
     1981 tagctgaatt tgaattgccg caccatggaa ctcaaggcca ggaatgctgc tgtcaataaa
     2041 atgactgtcg atacagagta aaataagctt ccccacgggt agcgtacggc ttgttccagt
     2101 cgcagcggca atggggtgtc ggtatttaac ggaaggggtt ttactgtcaa taaaaggtgt
     2161 tgatgctgcc tgtaatgaaa tgcccatatg aataagggca atactatcca aaaccaagac
     2221 agaggtagtg tcggttgcca taagttcagt atagccatca caagcaaaac acccaatccc
     2281 acacacactg tccgtcctag cggagaaggc aagctgcgtt gttcaagtac acacacccat
     2341 aagagccata acaaagcgca gagccaagca tggggaagtt tgccaatcag catgcaaatg
     2401 gccaatactg cggcgctggt aatcaagcct atgggctgta aataaaaggg gagcatgggt
     2461 ttcatgatga tggcggctat gagcgagaat ttgctgtggt tgtagcagca agtatgccgt
     2521 ggtaatgcaa agtatgtggc aagctaaaca agcggcctgt aaaccccttg ataggtttac
     2581 aggccgcttg tttgtagttt gtttcaattg taaaaatgaa aaactattgc cctttgacca
     2641 tttgcatgat tttgttcaaa tccaaggttt tggcttgttg ttggatttcg gtttggtatt
     2701 gtggctgtaa gcctatgccg tcttgcatca ggcgggcgta ggcatctttt aagactttag
     2761 ggctgaattg gcggcccgaa gcgtaatact ctttggccac atgacccaaa gcgtaagtgg
     2821 tggcaaaact catgcctgag ctgatggctt ggttgcctac tttgccgacc atgcctgtga
     2881 gggatttgcc cagtttgcca cccaagctgg agcgcaaacc gcccaatacc gtacccagca
     2941 gcttgatgcc tgcttgctcc aagtattgtg aggtcatgcc tatgcccagg gtggcgagca
     3001 aatctttgat gtggccttgg tccaaatcga agccaaaatc cttgcctatg ccatacacca
     3061 agcgcatttg tagcgggata atcgccatgc tggcggcggt ttgcggcagc aattctaggg
     3121 caccattgat gatggcggct tttaaaatgc gtttgttcag cgtttcttca agttggctgt
     3181 cgatttgcac cagacttttc ggtgcggctt cggcaatggc tgcgcctgcg agcgcgactt
     3241 cgtgtaagcc ttggttgaat tgttgttggt tgctgacact gagctcggtg ttggctatgg
     3301 cctggcttgg ggtggcgctc aataaaggcg caccatccaa ttgtaaaacc tgtgccaaac
     3361 ggctgaggaa ggcttgttcg gcttcactga tgctgccatc ggcttcgcac acacctaaag
     3421 caatttcgta ggccaattgt ttggagccgc catcgctgat gtcggcggca atttcttcta
     3481 acacggcttg ttggcgcaac acggcttggt ctatttgtaa caattgcact ttgttgtcta
     3541 agcccaggct ggaggcgatg gcgctgagtt cattgcgttc actgctgtgt ttgctgccat
     3601 cggcaaaggc ggccatgacg gccagggtca tgatggctta gttttcggta tcgctcatgc
     3661 gggagtctcc tgtggttgcg cggtatcgat attcaaacag atgggtggtg cggggcaaag
     3721 ttcaagctta aagttaggta aaggcacctt gatgaagcgg cctgtaaagc cgctgtgggc
     3781 ttcattcagg gcgacagtct tgcactgaaa tgtgtgcggt gatgaattgg gcatcggttt
     3841 gcaaacgtac cacacaacgc tgccatcgca tggttttggg cgttaatggc atgctgacca
     3901 gtgctgctga atcttggctg gaggtctctt ttgacggcga gtaggcggga atgaggtcgc
     3961 cttgatggtt aatcaacaca tggactgctt gccaagacag taccgctcca gtagcagaag
     4021 gcggtgaacc gtggcttggg tggccaaaat aagtc
//
LOCUS       DAOMVK010000174         4011 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_6480_length_4011_cov_3.684024, whole genome shotgun sequence.
ACCESSION   DAOMVK010000174 DAOMVK010000000
VERSION     DAOMVK010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6480_length_4011_cov_3.684024"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            601..870
                     /locus_tag="PK856_06655"
     CDS             601..870
                     /locus_tag="PK856_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02085.1"
                     /translation="MKKTIITTVLAALSVSTAMAASIANFTNATEQQNPYAQSNAQVQ
                     SQAVELEQVNYGKVNGKVVNPLFKYTGQSTASQVSAETSSVRVSH"
     gene            complement(992..1462)
                     /locus_tag="PK856_06660"
     CDS             complement(992..1462)
                     /locus_tag="PK856_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02086.1"
                     /translation="MKVWNTKAVAVLALLLPLSALAIPPVTSLPDSTKLSMPAGFQGH
                     WASALDVANAHKTCRNQYHGDDAATVKIDAARNRIHVQFYENEAQLRWLSFKDKTATQ
                     VRGDLRTVTRYEGDDADTRFTTSAQMVLQNNGRQILTKNVLQNNTTRVLVFCGR"
     gene            complement(1723..2301)
                     /locus_tag="PK856_06665"
     CDS             complement(1723..2301)
                     /locus_tag="PK856_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02087.1"
                     /translation="MKKTSITLLGLAAVLALSGCQNGVVATPFGDYGVKSRDGNRVTI
                     GFINKKTPQLGGNAKTVAKAGKPKIVMPEGFNGYWVAADNLKDRGAVCRGTYLGDSGA
                     HLNIDAKNNAIDLGFYEEGALIYWTSMQQHDPKRVSGQINYLYQGQGDGESEPMLVNI
                     SMQLINNGKELVAKNLVGENQTTTFLRCHKKQ"
     gene            complement(2511..>4011)
                     /locus_tag="PK856_06670"
     CDS             complement(2511..>4011)
                     /locus_tag="PK856_06670"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRL02088.1"
                     /translation="IEPKPTSHGNKRQINQSTCNKDFSCVKGFCPSFVTLNGAQLRPP
                     KVSEQKFDVAALPEPVLPQLDHAYGIVITGVGGTGVITVGALLGMAAHLEQKGVTVLD
                     MTGLAQKGGSVFSHVQIAASPDKIHATRIATGEADLLLGCDAIVAGSVEALSKLQKGV
                     SHAVVNRAPIPSAEFITNADWTFPSGSVERELQAALGDACEFFDSNVLAVKLLGDSIY
                     ANPLLLGYAWQKGWIPLTCASILQAMTLNAVQVEKNKQAFEYGRLAAHDLHGLMQQLQ
                     LPTEAPKAPTLDDIMAEETAFLTAYQSGRYAQRYREVVQQIRANEQRAGVDTTLPLSM
                     AVARNLAKLMAYKDEYEVGRLYSRPEFLNSLRQQFAGEPGVDYQVQYHLAPPALGKRD
                     ADGHLIKQDFQNKWIAPLFKALAVLKPLRGTPFDPFGYTEERKAERRWIEDYIADMQK
                     ISAALNQRNLPLALEWAALPQQIRGYGHVKEKNMHAALLKREHFLKQAL"
BASE COUNT          996 a         1106 c          910 g          999 t
ORIGIN      
        1 gccacacatg gcttggggca gccaagaggc gatgacgcgc ttgttccaca tgctcatcga
       61 caacatcaac aaaggcatgg caggtacacc acaaagcgtg gttgtgcgca aataaacttg
      121 gtcgtgcttt agtgcgtgtc tgaggctctt ctgccatcat atggtgcgaa ccatgacaat
      181 gcagcctgta aacttgaatg ggtttacagg ccgcatttca atcttgtttc attaccaagc
      241 cttatgtcct ttatgcagca tgccccaagc cactgacatc cgcaccattt acctacttct
      301 taatcatgtc aacaacacac cctgtttgaa tttgcacatc attcatctgc tttaaagcat
      361 ctaagtgata taaaaagcgg cctgtaaacc gaaatatcat gacttacagg ccgcttaatt
      421 ctttgaaccc acaataaaaa ctgctgttta ctgtacctga aatcaacact gggcttggct
      481 ttgctagatt gttaacttta agttaacatt gttttgaata tatgcctatt ttttaaacca
      541 ccgagaatcg ataagatgca gtcatcgaaa caacaaaaca acaactaaag aggcaagcaa
      601 atgaaaaaaa ccatcatcac caccgtattg gccgcattga gtgtttctac tgccatggca
      661 gcgagcatcg ctaacttcac caacgccacc gaacaacaaa acccatatgc ccaaagcaat
      721 gcccaagtgc aaagccaagc agtggaattg gaacaagtga attacggcaa agtgaacggc
      781 aaagtcgtga acccattgtt caaatacacg ggccaaagca ctgccagcca agtgtctgca
      841 gaaacctcat cagtgcgtgt aagccactaa gcaaagcgtt atcacacttt ggtaacccct
      901 tactcctgaa gccaaagtga tacaaagaca agtggcctgt aaacatttac aggccgcttg
      961 ttgatttcat gccatgacac caagacccac actaacgtcc acaaaacacc aacacacggg
     1021 tggtgttatt ctgtaacaca ttcttggtca aaatctgacg accattgttc tgtaacacca
     1081 tttgtgcgga agtggtaaat cgggtgtcgg catcatcgcc ctcataacgc gtcaccgtgc
     1141 gcaaatcacc acgcacttgt gtggccgttt tgtccttgaa actcagccaa cgtaactgcg
     1201 cttcgttttc gtaaaattgc acatggatgc ggttgcgggc agcatcaatc ttcacagtgg
     1261 ccgcatcgtc accgtgatac tgattgcgac aagtcttgtg ggcattggcc acatccaaag
     1321 ccgaagccca atgaccttga aaaccagcag gcatagacag cttcgtgcta tcgggcaatg
     1381 aggtcacagg tggtatggcc aaagcgctca agggcaataa caaggccaag actgccaccg
     1441 cttttgtgtt ccaaaccttc atctttactc cttcatcatt ggattgtgtg cccacactaa
     1501 cacagcccaa gcaacacaac agccacctta ttgatgcggc ctgcacacgc cactgggcgt
     1561 agcaccatgt gcacatgcat ggattgccat gcccactcat cgccctagtt gcttatggcc
     1621 aagacagctt acaggccgct tgtttacgct cacaacaccg acactgctac tacatcgtta
     1681 ctccgaacag atttccatat tcctatcaat tggtgtacat gcttactgct ttttatggca
     1741 gcgcaaaaat gtcgtggtct gattttcacc tactaaattt ttagccacca attccttccc
     1801 gttattaatg agttgcatgc tgatgttaac caacatcggt tctgactcac catctccttg
     1861 cccttggtag agatagttga tttgcccact cacccgcttt gggtcgtgtt gctgcatact
     1921 ggtccaataa atgagtgccc cttcttcgta aaatccaagg tctatggcat tgttttttgc
     1981 gtctatgttc aggtgagcgc cactgtctcc gagatacgtc cctcggcaca cggctcctcg
     2041 gtcttttaag ttgtcggcgg caacccaata gccattgaat ccttcaggca tcacaatctt
     2101 aggcttgcct gctttggcta ccgtctttgc attgccacca agttgtggcg tttttttatt
     2161 gataaagcct atggtcacgc ggttgccgtc gcggcttttc acgccgtaat cgccaaacgg
     2221 cgtggccacc accccgtttt gacagccgct caaggccaag acagctgcca agcccagcaa
     2281 tgtgatggat gtttttttca tgattcccct tagtgttcca cagtatgacg tattccttaa
     2341 aacaagcaga cggcatacca aatcaccgcc catgtgccgt ctataacgca catcatgttg
     2401 cctttgcatg aaaaccatac cttggcaaca aaccaatcgc ttcaacacgc aaaaaagcgg
     2461 cctgtaagca tttacaggcc gctgatttca ctgttgccta tgcggtttgc tcacaaggct
     2521 tgtttgagaa aatgctcacg cttgagcagc gcggcatgca tgtttttctc tttcacatgg
     2581 ccatagccgc gaatttgctg tggcaaagcc gcccattcca aagccaaagg caggttgcgc
     2641 tgattcaaag ccgcactgat tttttgcatg tcggcgatgt aatcttctat ccaacggcgc
     2701 tcggctttgc gctcctcggt ataaccaaac gggtcaaacg gcgtgccgcg caaaggtttc
     2761 aacaccgcca aagccttgaa caagggcgcg atccatttgt tttgaaagtc ttgcttgatt
     2821 aaatgcccat cggcatcgcg tttgcccagc gcaggcggtg ccaaatggta ttgcacttgg
     2881 taatccaccc caggctcgcc cgcaaactgt tggcgcaagc tgttgagaaa ctcggggcgg
     2941 ctgtacaagc ggccgacttc gtactcgtct ttgtacgcca tcaacttggc caaattgcgg
     3001 gccaccgcca tgctcaatgg caaagtggtg tccacacctg cgcgttgctc attggcgcgg
     3061 atttgctgta ccacttcacg ataacgctgc gcatagcggc cactttggta agccgtgaga
     3121 aaagctgttt cttcggccat gatgtcgtct aacgttggcg ctttgggcgc ttcggttggc
     3181 agctgcaatt gttgcatcaa gccgtgcaaa tcatgggcgg caagacggcc gtattcaaaa
     3241 gcctgtttgt ttttctccac ttgcaccgca ttcagcgtca tcgcctgcaa gatagacgcg
     3301 cacgtgagcg gtatccagcc tttttgccac gcatatccga gcaacagagg attggcataa
     3361 atgctgtcac ccaaaagctt cactgccaac acattgctgt cgaaaaactc acacgcatcg
     3421 cccaatgcgg cctgtaactc gcgctctacg ctgcccgatg gaaaggtcca atcggcattg
     3481 gtgatgaatt ctgccgacgg tatcggcgcg cgattgacca cggcatggct cacgcctttt
     3541 tgcagcttgg acaaggcttc taccgagccc gccacaatgg catcgcagcc caagagcaaa
     3601 tcggcttcgc ctgtcgcgat gcgcgtggcg tgaatcttgt cggggctggc ggcaatttgc
     3661 acatggctga acaccgagcc acctttttgc gccaagcctg tcatgtcgag cacggtcacg
     3721 cctttttgct ccaaatgcgc cgccatgccg agcaaagcac cgacggtaat cacacccgta
     3781 ccgcccacac ccgtaatcac gatgccgtaa gcatggtcga gttggggcaa gacaggctca
     3841 ggcaaggccg ccacatcaaa tttctgctcg ctcactttgg gcggacgcaa ttgcgcgccg
     3901 tttaaggtga caaagctggg gcaaaagcct ttcacacaag aaaagtcttt gttgcaggtt
     3961 gattggttga tttggcgctt gttgccatga ctggtgggtt taggctcgat g
//
LOCUS       DAOMVK010000175         3982 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_6548_length_3982_cov_3.880316, whole genome shotgun sequence.
ACCESSION   DAOMVK010000175 DAOMVK010000000
VERSION     DAOMVK010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6548_length_3982_cov_3.880316"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="PK856_06675"
     CDS             <1..398
                     /locus_tag="PK856_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRL02089.1"
                     /translation="FLDAGMGNVLAFFELPTQPDMGRDENTPKWVQHLAMKVKDRPTL
                     EAAKAHLESKGVDVLGITNHGIFHSIYFFDPNGHRIELAYDDEKAAEKVARITEEMKV
                     EMLNEWDTTKRAPHHTHFLHVDELDESKT"
     gene            640..945
                     /locus_tag="PK856_06680"
     CDS             640..945
                     /locus_tag="PK856_06680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HRL02090.1"
                     /translation="MLKLIAEDFIHVDKIEVVLPLYQALIEHTRLETGCLAYDLYHDL
                     KNPGHFVFIEEWENRAALDAHVQSEHFQRLVPLIDAHKRAEGVFTHLQNYEALLPCS"
     gene            complement(1029..1286)
                     /locus_tag="PK856_06685"
     CDS             complement(1029..1286)
                     /locus_tag="PK856_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02091.1"
                     /translation="MKSTIMLLGLAMLLTACADNTKADASLSKHCQAAYEAYLKQLEA
                     LPPETVKHATATTGVSVEEAKKLTRQMYADMSDEECQQALP"
     gene            1429..2115
                     /locus_tag="PK856_06690"
     CDS             1429..2115
                     /locus_tag="PK856_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02092.1"
                     /translation="MNKPIQFLFILSLSVMLLACGKDANHQNKSHAYDYRDERHQDFY
                     SRYMRKTQQDYLIIESKNIREKLKLNDVPDEVLARMDVSRTEPTEKIIPKINQLSNRA
                     EILAFFNRNGGTFKECVVEDQEEHFFCIEDEKLGLSSRKYIYIPNESIKLKHIDQPIK
                     MKCSMFQDRKSCSIIDRFNYPYNARLTIHFTDIQNFFYIIPYIEQHFYETTGEHVLEN
                     APPSHASAAQ"
     gene            complement(2252..3781)
                     /locus_tag="PK856_06695"
     CDS             complement(2252..3781)
                     /locus_tag="PK856_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HRL02093.1"
                     /translation="MEFLLDPNIWAGFITLVVLEVVLGIDNLVFIAILADKLPPEQRD
                     KARVIGLSLALIMRLALLSVISWLMALTNPLFNIAGLDFSGRDLILLFGGLFLVYKAV
                     SELHENMEGKPHAHTSNAAYAAFGAVVAQIVVLDAVFSLDAVITAVGMVDNIYVMMAA
                     VVVAVAIMLIASKPLTKFVNAHPTVVVLCLSFLLMIGFSLIAEGLGFHIPKGYLYAAM
                     GFAILIEVFNQVTSRNKAKHQAHIPRRLRTTEAVMRIMGGKTAAETEASVAKEADKAD
                     FVVEERYMIGGVLTLGERSVASIMTPRNHISWVDLDKGTEHMYEVLMSVPHSLFPVCR
                     GDLDHLVTVAKAKDLLDVLDDEAALQALLQAASKPVTVFEGMKVMDAIPSLRQAKGSL
                     VMVTDEFGSVTGLVTPLDVFEAIAGEFPDADESLDLVKVDDSHYIAKGSLDVYQLELQ
                     LGVSDLVDEDASYVNVAGLMLSEAESLPQIGMAVERGSLRFEVESMQDRSIDKVRISR
                     I"
BASE COUNT         1120 a         1029 c          850 g          983 t
ORIGIN      
        1 tcttcttgga tgcgggcatg ggcaatgtgt tggcattttt tgaattgccc acccaacctg
       61 acatgggccg cgatgaaaac accccgaaat gggtgcaaca tttggccatg aaagtgaaag
      121 accgcccaac tttagaagcc gccaaagcgc atttagaaag caaaggcgta gacgtgcttg
      181 gcatcaccaa ccacggcatt ttccactcga tttacttttt cgaccccaac ggccaccgca
      241 tcgaattggc ttacgacgat gaaaaagccg cagaaaaagt cgcgcgcatt accgaagaaa
      301 tgaaggtgga aatgctcaac gaatgggaca ccaccaaacg tgccccgcac cacacgcatt
      361 ttttgcatgt cgatgaattg gatgaaagta agacctgagt ccaacccttc tacggattca
      421 ggattaagag actgcgcagc agcctctttt ttttattttt tggccttatt acggcttatt
      481 tagcattgca tgctcatgca agcggcctgt aaatggcatt tcaccctcaa ttaaacatga
      541 cacattgact ggctccaccc tactgccgca caaggacatg aaaaaaactg gcgtttcagt
      601 taaggtagat ggccaaacgg ccaaaacaag gaaatgctca tgctgaaact gattgcagaa
      661 gatttcatac acgtcgataa aattgaagtt gtcttgcctt tataccaagc cctcattgaa
      721 cacacccgac tcgaaacagg ctgtcttgcc tatgacttgt accatgactt aaaaaaccca
      781 gggcattttg tgttcatcga agaatgggaa aatcgagcgg cattggatgc gcatgttcaa
      841 tccgagcatt ttcaacggtt ggtgcctttg attgatgccc acaaacgtgc agagggcgtg
      901 ttcacccatt tgcaaaacta tgaggcctta ttgccctgtt cttgatggca atacccccat
      961 tcatgtgctt gtggctcagc cgccactcat gcagctcata ccttccttct catcaaagtg
     1021 ttgccgcctt aaggcaatgc ctgctgacac tcctcatcac tcatgtccgc atacatttgg
     1081 cgcgtgagct tcttcgcctc ctccacactc acacccgtcg tggcggtggc atgtttcact
     1141 gtctcaggcg gcaacgcctc tagctgtttc aaataagcct catacgccgc ttggcaatgc
     1201 ttgctcaagc ttgcgtctgc ttttgtgttg tcagcacaag cggttaacag cattgccaag
     1261 cccagcaaca tgattgtcga tttcatttcc ctatctgccc tttgtgattg ttgataattt
     1321 gtatatccca tgttgatgat tttattacct gcacccatgg caagctttac cctgcaagca
     1381 tgattgcgta tcctagattt tttacaccaa ttttaatcac ggcctgccat gaacaaaccc
     1441 atacagttct tattcatatt gagcctgtct gtcatgctgc tggcttgtgg caaagatgcc
     1501 aaccatcaaa acaaaagcca tgcttacgat taccgtgatg aacggcatca ggatttttat
     1561 tcacgttaca tgcgcaagac ccaacaagac tatttgatta tcgaatccaa aaatatacgt
     1621 gaaaaattaa aattgaacga tgtacctgat gaagtgcttg caagaatgga cgtgtctcga
     1681 acagaaccta ctgaaaaaat tattcccaaa atcaatcaac tcagtaatcg agctgaaatc
     1741 ttggcttttt tcaatcgtaa tggcggtaca ttcaaggagt gtgtagtaga agatcaagag
     1801 gaacattttt tctgtataga agatgaaaaa ctgggccttt ctagtcggaa gtatatttac
     1861 attccaaatg aatctatcaa gctaaaacac attgatcaac ctattaaaat gaaatgttct
     1921 atgtttcagg ataggaaaag ttgctcaatt atagataggt ttaattatcc ttataacgca
     1981 aggcttacca ttcatttcac agatattcaa aattttttct acattattcc ttatattgaa
     2041 cagcattttt atgaaaccac gggcgagcat gtgttggaaa acgcgccacc atcccatgca
     2101 agcgctgccc aataaagtat aaaaccgcca caagcggcct gtaaatcaat cgtccatatt
     2161 tacaggccgc ttaaaaatac cgctctatca aaatgcctat ccatacaaaa agccgacgca
     2221 agcgtcggct tttgatgaca tcactacaag cttaaatgcg gctgatgcgc accttgtcta
     2281 tgctgcggtc ttgcatggac tccacttcaa agcgcaagct gccgcgctca accgccatgc
     2341 cgatttgtgg caggctttcg gcttcggaca acatcaaacc tgcaacattg acgtagctcg
     2401 cgtcttcatc caccaaatcg ctcacaccca attgcaattc caattggtag acatcgagcg
     2461 agcctttggc gatgtaatgg ctgtcgtcca ctttgaccaa atccaagctt tcgtcggcat
     2521 cggggaattc gcccgcaatc gcttcaaaca cgtccaaagg cgtgaccaaa cctgtgaccg
     2581 aaccaaactc atccgtcacc atcaccaaag aacctttggc ttgacgcaag ctcggtatcg
     2641 cgtccattac cttcatgcct tcaaacacgg taacaggctt agaagcggcc tgtaacaagg
     2701 cttgcaatgc agcttcgtcg tccaacacgt ctaacaagtc tttggccttg gccaccgtca
     2761 ccaaatggtc taagtcgcca cgacacacgg gaaacaggct gtgcggcact gacatcaaca
     2821 cttcgtacat gtgctctgtg cctttgtcca aatccaccca cgaaatgtgg ttgcgtggtg
     2881 tcatgatgct ggccactgag cgttcgccca aggtcaatac cccgcctatc atgtagcgct
     2941 cttccaccac aaagtcggct ttgtcggctt ctttggccac tgaggcttcg gtttcagccg
     3001 cggttttgcc acccatgatg cgcatcactg cttcggtggt gcgcaaacgg cgcggaatgt
     3061 gtgcttggtg tttggctttg ttgcgcgagg tgacttggtt aaacacctca atcaaaatgg
     3121 caaaacccat ggccgcatac aaatagcctt taggaatgtg gaagcccaag ccttcggcga
     3181 tgaggctgaa accaatcatc aacaagaagc tcaagcacaa caccaccacg gtagggtgcg
     3241 cgttcacaaa cttggttaaa ggcttggaag caatcaacat gatggccact gccaccacca
     3301 ccgccgccat catcacgtaa atgttgtcta ccatgcccac ggcggtaatc accgcgtcga
     3361 gcgaaaacac cgcatcgagc accacaattt gcgccaccac cgcaccaaac gcagcataag
     3421 cggcattgga tgtgtgggcg tgcggcttgc cttccatgtt ttcatgcaat tcggacacgg
     3481 ctttgtaaac caagaacagg ccaccaaaca acaaaatcaa atcgcgacct gagaaatcca
     3541 aacccgcgat gttgaacaaa ggattggtta aggccatcag ccatgaaatc accgataaca
     3601 aagccaaacg catgatgagc gccaacgata aaccgatgac acgggctttg tcgcgttgtt
     3661 cagggggaag tttgtcggcc aagatggcga tgaagaccag attgtctatg cccagcacca
     3721 cttccaacac caccaaggtg atgaaaccgg cccagatatt ggggtctagc agaaattcca
     3781 ttattgaacc ctccaagcgg cctgaacagg cacagggtca atcacaacag gaatgggtaa
     3841 atcagagaaa acagcaaaca gataaaacga tgtcgaagta agactcaaac gcttcggcga
     3901 ggggtccata taaaccttat gttgtggaaa gtgtgcatag tttctctaaa acagaatgtg
     3961 gaatcaatag gttttaaaat tc
//
LOCUS       DAOMVK010000176         3978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_6558_length_3978_cov_3.242162, whole genome shotgun sequence.
ACCESSION   DAOMVK010000176 DAOMVK010000000
VERSION     DAOMVK010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6558_length_3978_cov_3.242162"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..1142)
                     /gene="pyrC"
                     /locus_tag="PK856_06700"
     CDS             complement(96..1142)
                     /gene="pyrC"
                     /locus_tag="PK856_06700"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRL02094.1"
                     /translation="MTERLTLIQPDDFHVHFRSGAALQAVVPHTAHQMARALVMPNLK
                     PPVTQVAEALAYRDEILAAVPTGVAFTPLMSLYLTDKTDVATVTAAKAAGIVAFKLYP
                     AGATTNSDSGVTDLFKLLPVLEAMAEQGMLLLVHGEVTHSHIDIFDREAVFIDEVLLP
                     IMAKVPSLKIVFEHITTSEAAQLVQSAGENLVATVTPQHLLHNRNDMLVGGVRPHLYC
                     LPILKRRTHQEALLAAVSGAQAHKFFLGTDSAPHEQGTKECACGCAGVFSALTAMELY
                     AEAFESVNALDKLEAFASQNGARFYGLPENSRKIVLEKRPQAVPEYVDYVTGRLIPMR
                     AGESLAWSFAGFAD"
     gene            complement(1221..1451)
                     /locus_tag="PK856_06705"
     CDS             complement(1221..1451)
                     /locus_tag="PK856_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009116436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HRL02095.1"
                     /translation="MAQQASLDVKGLKCPMPMLRAKKALAQLESGDELTVYATDPGVQ
                     EDFAAFCRHTGHVLLQSTVNEDESFTLILRHK"
     gene            1577..2167
                     /locus_tag="PK856_06710"
     CDS             1577..2167
                     /locus_tag="PK856_06710"
                     /inference="COORDINATES: protein motif:HMM:NF020331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HRL02096.1"
                     /translation="MSYRFAHLSALVLLCTSLTTLAAERKEFNQNIKEADYSFQQQTI
                     NLPDYPAANGKWVDLYVSPTFAGKPKILLGSIHIHDNNSLSYVLNNQSGNGNDNISFE
                     NLRCSVQSFRSEYNNGFRQIAFADTYNKRWLESKNAPWRDVGSNLSTTTPVQEMLSRV
                     FCDDGMPRNDAELTQRLIDRGTFSAVQNRGTGGSNK"
     gene            2180..2956
                     /locus_tag="PK856_06715"
     CDS             2180..2956
                     /locus_tag="PK856_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRL02097.1"
                     /translation="MSDLILMCVLAFLAGMMDAAVGGGGLLQIPSLFSLMPANTPIAT
                     IMGTNKFASVCGTLTSGWQYVRKIPVPWKMLLPAAVLAFLTSFGGSMLVSYIPVQWFK
                     PFILLVLVVMAVYTFCKKDFGQNQRSSALSKNEWYVGLACGALIGFYDGLIGPGTGSF
                     LTFMFIRLFAFDFLTAVASSKIINITTNLAALSFFVPHGYIFWAWAIPLAAANLCGGF
                     TGTRIALKGGTQFLRYGFMVLLVILIGKFAWDLLPLLHAA"
     gene            3101..>3978
                     /locus_tag="PK856_06720"
     CDS             3101..>3978
                     /locus_tag="PK856_06720"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRL02098.1"
                     /translation="MKRRHVSWLLGGLFIAGTQSLCAQPTPPAELSLSADPAALSRHI
                     TYLSSLSRHHENPAGLDTAAAYIKAQIEQFGLPCAYQNYSVHAQTYRNVVCTLNPDKS
                     DTVIVGAHYDSYRDLPGADDNASGTAGVIELARLLKAQEASLPQQLELVAYTLEEPPF
                     FRSEHMGSAVHAQSLSMRPVNVKAVVILEMIGHYSDEAVQDYPLGIMRAVYPKQGNFI
                     AAVGNWQSKKLTRQYCQHMQALQQLPCERLTAPSRLTGVDFSDHLNYWARKIPAIMLT
                     DTSFYRNKNYHTEHDT"
BASE COUNT          948 a         1128 c          940 g          962 t
ORIGIN      
        1 ttcctcgcgt tgcgatgccc acacccaact gggtgtcagc gccaaagcca aagcggcgct
       61 gatgacggtg cggcggcggt ggttgatgcg gctcatcagt cagcaaaacc agcaaacgac
      121 caagccaagc tctcgcctgc acgcatgggt atcaagcggc ctgtaacata atccacatac
      181 tcaggcactg cttgtgggcg tttttccaac acgattttgc ggctgttttc aggcaagcca
      241 taaaaacgcg cgccattttg cgaggcaaag gcttccagtt tgtccaatgc gttcaccgat
      301 tcaaacgcct cggcgtacaa ctccattgcc gtcaaagcgc tgaacacgcc cgcgcagcca
      361 cacgcacatt cttttgtgcc ttgctcatgc ggcgcactgt ctgtgcccaa gaagaatttg
      421 tgtgcttgcg cgccgctcac ggccgccaac aaagcctctt ggtgggtgcg gcgcttcaaa
      481 atcggcaagc aatacaaatg tggacgcaca ccgccgacca acatgtcgtt gcggttgtgc
      541 aataaatgtt gcggcgtcac tgtggccacc aagttctccc ccgcgctttg caccaattgc
      601 gccgcttcgc tggtggtgat gtgttcaaac acaattttca aactcggcac tttggccata
      661 atcggcaaca acacctcatc aataaacacc gcttcgcggt cgaaaatatc aatgtgcgag
      721 tgcgtgactt caccgtgcac caacaacaac atgccttgct ccgccatggc ttcaagcaca
      781 ggcaacagct tgaacaaatc ggtcacgccc gaatcagaat tggtggtcgc gcccgcagga
      841 tacaatttga acgcgacgat gcccgctgct ttcgccgccg tcacggtggc cacatcggtt
      901 ttatcggtta aatacaaact catcaatggg gtaaacgcca cacctgttgg aacggccgcc
      961 aaaatttcat cacggtaggc caaagcctct gccacctgag tcacaggcgg tttcaaattc
     1021 ggcatcacca aagcgcgggc catttggtgc gccgtgtgtg gcaccacggc ttgtagcgca
     1081 gcaccgctgc gaaagtgaac gtgaaaatca tctggctgta tcagggtcag acgttcggtc
     1141 attgctattc ctttaattga atgtgtggcg cagtgtggtt tactggccgc ttgtatctgt
     1201 atgtatgcgg cctgcaaacc ttatttgtgg cgcagaatca gggtaaagct ttcgtcttca
     1261 ttgacggtgg attgcagcaa cacatgccct gtgtgacgac aaaatgccgc aaaatcttct
     1321 tgcacacctg ggtcggtggc gtaaacggtt aattcgtcgc cgctttccaa ttgtgccaag
     1381 gctttttttg cccgcaacat cggcatcgga cattttaagc ctttgacatc caaacttgct
     1441 tgttgggcca taattcattc tttcaaacgc aaaacggaca aattatagca ttgttaacgg
     1501 cactcacaca gccgcacatg tgggtgcaaa cttgcgttac aatgcccact gccacatatc
     1561 cgcataggaa tttacgatgt cttatcgttt tgcccactta tctgccttgg tcttgttgtg
     1621 caccagcctg acaacgcttg ccgcagaaag aaaagaattc aatcaaaaca tcaaagaagc
     1681 cgattactca tttcaacaac aaaccatcaa tctgcccgac tatccggcag ccaatggcaa
     1741 atgggtggat ttgtatgtga gccccacttt cgcgggcaag cccaaaattt tgctgggcag
     1801 catccacatt cacgataaca attctttaag ctatgtcttg aacaaccaat cgggcaatgg
     1861 caacgacaac atcagctttg aaaacctgcg ttgctcggtg caatccttcc gctccgaata
     1921 caacaatggc ttccgccaaa ttgcctttgc cgacacctat aacaagcgtt ggttagaaag
     1981 caaaaatgcg ccatggcgcg acgtaggcag caatttaagc accactaccc ccgtgcaaga
     2041 aatgctcagc cgcgtcttct gcgacgatgg catgccacgc aacgatgcag aattaaccca
     2101 acgcttaatc gaccgcggca ccttcagcgc cgtacaaaac cgcggcacag gcggctctaa
     2161 caaataagct agccttccca tgagtgattt gattttaatg tgtgtgctcg cttttttagc
     2221 gggcatgatg gacgccgccg tgggtggcgg cggtctgttg caaattccgt ctttgttcag
     2281 cctgatgccc gcaaacacgc cgattgccac catcatgggc acgaacaaat tcgcctcagt
     2341 ctgtggcacg ctgacttcgg gctggcaata cgtgcgcaag atacccgttc cttggaaaat
     2401 gctattgcct gcggctgtgc tggcatttct cacctctttt ggtggctcca tgctggtttc
     2461 ctacattcca gtgcaatggt tcaagccgtt tattttgttg gtgttggtgg tgatggcggt
     2521 atacacgttt tgcaaaaaag actttggcca aaaccaacgc agcagcgcct tgagcaaaaa
     2581 cgagtggtat gtcggcttgg cttgtggcgc cttaatcggt ttttacgatg gcctgatagg
     2641 gccaggcaca ggcagttttc tcaccttcat gttcatccgt ttgtttgcgt ttgactttct
     2701 caccgccgtg gcctcctcca aaatcatcaa catcaccacc aatttggccg ccttgagctt
     2761 ttttgtgccg catggctata ttttttgggc ttgggcgatt ccgttggcag cagccaattt
     2821 gtgcggcggt ttcacgggca cacgcatcgc cttaaaaggc ggcacccaat tcttgcgcta
     2881 tggctttatg gtgttgctgg tgattttaat cggcaaattt gcgtgggatt tgctgccctt
     2941 attgcatgcg gcctgaaaag caactgctga ttgaatccat tttaagtagc aagcgatgcg
     3001 accttgagtc gcattcttta tcgccgttca ttttgtgttg gctgcgtccc aacaccaccc
     3061 gctagattgc caagcattgc acccatcttt ggaaaaaaca atgaagcgca ggcatgtatc
     3121 atggttgttg ggcggcttat tcatcgcggg aactcaaagc ctgtgtgctc aacccacacc
     3181 accagcagaa ctgagcctca gcgcagaccc agccgcttta tcaagacaca tcacctacct
     3241 cagcagcctg tcacgccacc atgaaaaccc tgcaggtttg gacacggctg ctgcttacat
     3301 caaagcccaa atcgagcaat tcggcttgcc ttgcgcctat caaaactaca gcgtccatgc
     3361 ccagacttac cgcaatgtgg tctgtaccct caatccagac aaatctgata ccgtcatcgt
     3421 cggcgcgcat tacgacagct accgtgattt gcctggtgcc gatgacaatg cctcgggcac
     3481 cgcaggggtg attgaattgg cacggctctt aaaagcgcaa gaagcctcgt tgccgcaaca
     3541 gcttgaattg gtggcttaca ccttggaaga gccgcctttc tttcgctccg aacacatggg
     3601 cagcgccgtg catgcacaat cgctatccat gcggcctgta aacgtgaaag cagtggtgat
     3661 tttggaaatg ataggccatt acagtgatga agccgtacaa gactatccct tgggcatcat
     3721 gcgcgccgtc taccccaagc aaggcaattt catcgccgcc gtgggcaatt ggcaatcgaa
     3781 aaaactcacc cgccaatatt gccaacacat gcaagcctta cagcaattgc cttgcgaacg
     3841 cctcaccgcg cccagccgcc tcactggcgt cgatttttcc gaccatttga attactgggc
     3901 cagaaaaatc cccgccatca tgctcaccga cacctcgttt taccgcaata aaaactacca
     3961 caccgaacac gacacctt
//
LOCUS       DAOMVK010000177         3976 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_6566_length_3976_cov_3.491201, whole genome shotgun sequence.
ACCESSION   DAOMVK010000177 DAOMVK010000000
VERSION     DAOMVK010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6566_length_3976_cov_3.491201"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /gene="mutL"
                     /locus_tag="PK856_06725"
     CDS             <1..1070
                     /gene="mutL"
                     /locus_tag="PK856_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957472.1"
                     /note="This protein is involved in the repair of
                     mismatches in DNA. It is required for dam-dependent
                     methyl-directed DNA mismatch repair. Promotes the
                     formation of a stable complex between two or more
                     DNA-binding proteins in an ATP-dependent manner without
                     itself being part of a final effector complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HRL02099.1"
                     /translation="PAFVLNISLPPEQVDVNVHPTKSEVRFRESQSIHRLVFHALHQA
                     LAQTDATHTESVSNAGEILQQVLNTPEPSQNRFDWGQQRPVNPNTDHAPRSHTPVGQY
                     QASSSRPLSLRESQAALDTYAKLYQGNGSDNNVLQAALERSETLDLPTSQTDTDHNTT
                     ESEHPLGYALAQLLGIYILAQSADGLILVDMHAAHERVTYEKLKQQRDGGKLATQQLL
                     IPHSFAASALEVASVEQFQQELADWGLELSLLGASIAIRAVPVLLARGNVEALVRDSL
                     AELAQVGNSDALLAAENQLLATMACHGSVRAGRQLTLPEMNGLLRDMEQTPRANQCNH
                     GRPTWVKLKLGDLDALFLRGQ"
     gene            1306..2082
                     /locus_tag="PK856_06730"
     CDS             1306..2082
                     /locus_tag="PK856_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02100.1"
                     /translation="MLLTPVFLIKLFLTPVLMLGVSIAAKKWGTFWGGLMSGLPLTSG
                     VILTFLAVEQGAGFTQTAILGAIPGLAAVMAAYCVYASLSKRHALGLTGTAALLTFGL
                     LSWALSHLHSVGLSVAVLLLLIFLLIRKTGREYTPQNLIIQQNPWDVPLRMGASTALL
                     LLITTAAPHLGSSLSGVLSTIPVIAWPLMLFTHVQYGRNEMLAVVRGNAIGALRVLSF
                     YAVMYHSLPVLPLLWVVVLAALASVILPLLLSKTLPRKSA"
     gene            complement(2225..2659)
                     /gene="cadR"
                     /locus_tag="PK856_06735"
     CDS             complement(2225..2659)
                     /gene="cadR"
                     /locus_tag="PK856_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Pb(II)-responsive transcriptional
                     regulator"
                     /protein_id="HRL02101.1"
                     /translation="MQVQIGELAKRSDCKVVTIRYYEKEGLLPEPLRSSGNYRLYDEG
                     HLERLQFIRHCRSLDMSLEEIKTILGLRAAPQQDCAEVNAMLDAHIISVEERIVALLH
                     LKQHLLDLREKCGGVKTMATCGILQSLSDGSCHNELGLEGES"
     gene            2878..>3976
                     /locus_tag="PK856_06740"
     CDS             2878..>3976
                     /locus_tag="PK856_06740"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019486313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRL02102.1"
                     /translation="MDCPTEEALIRKKLASMDGIVALDFNLMQRVLNVSHTLPDTLAI
                     EHALAQIDMQAVKQTAALTTSRIAIQEMDCPTEEGLIRKKLAGMDGIDDLQFNLMARV
                     LTVSHAPVVLPNIVAALQSLTFNPKVLDSDTAATASDTPAPSPEKPYKKYLLAAALVA
                     AIAAEAVNFAGGSTVLEMALAAFAILASGLKTYQKGWIALKNFNLNINALMAIAVTGA
                     VIIGHWPEAAMVMVLFTIAELIEAKSLDRARNAIRGLLDLSPEKATVLQADGTWQETL
                     ASDVAVGSTVRVKPGERLALDGVLSKGSSSINQAPITGESLPVEKAVGDAVFAGTINE
                     AGSFEYQVNAAASHSTLARIIAAVESAQGSRA"
BASE COUNT          910 a         1129 c         1022 g          915 t
ORIGIN      
        1 cccctgcttt cgtgctcaac atcagcctgc cgcccgagca agtcgatgtg aacgtgcacc
       61 ccaccaaatc cgaagtgcgc tttcgcgaga gccaatccat ccaccgtttg gtcttccatg
      121 ctttacacca agccttggcg caaaccgatg ccacccacac cgaatcggtg agcaatgcag
      181 gcgagatttt gcaacaagtg ctgaatacgc ctgagccgag tcaaaaccgt ttcgattggg
      241 gccaacaacg gcctgtaaac ccaaatacag accatgcgcc acgctcgcac acgccagtgg
      301 ggcaatacca agcgagcagc tcgcgcccct tgagcttgcg cgaaagccaa gcggctctcg
      361 atacctatgc caagttatac caaggcaatg gcagcgacaa caacgtgtta caggccgcat
      421 tggagcgttc tgaaacctta gacttgccaa cgtcgcaaac agataccgac cacaacacca
      481 ccgaatcaga acacccactg ggctatgctt tggcacaatt gttgggcatt tacatcttgg
      541 cacaaagcgc cgatggcttg attttggtcg acatgcacgc tgctcacgag cgcgtgacgt
      601 atgaaaaact caagcagcaa cgcgatggcg gcaaactggc cacacaacaa ttgctgattc
      661 cacacagctt cgccgcctcg gccttggaag tggccagtgt tgagcaattt caacaagagt
      721 tggcagactg gggcttggag ctgagcttgt tgggcgcttc catcgccata cgcgccgtgc
      781 ctgttttgtt ggcacgcggc aatgttgagg ctttggtgcg cgactcgctc gccgagttgg
      841 cgcaagtggg caacagcgat gccttgctcg cggccgaaaa ccaattgctc gccaccatgg
      901 cgtgtcatgg ctcggtgcgc gcaggccgcc aactcacctt gcccgaaatg aacggtttgc
      961 tgcgcgacat ggaacaaacc ccacgcgcca accaatgcaa ccacggccgc cccacttggg
     1021 tgaaattgaa gcttggtgat ttggatgcgc tgtttttgcg cgggcaataa agcctagcaa
     1081 aataccgtta cccagttttt tctaacgggt tcacccattt acaggccgct tgtttgcaca
     1141 gcggcctgta aacattttga tgcaataaat acggccattt ctgctacaaa accttttacg
     1201 cgcgcaaacc agtccacatc atttgcccaa ctacccatca tgcctttgca caattcacca
     1261 cagcggtgat aatgggtctg tcattcctac ctgtctgcac accccatgtt gctcacccca
     1321 gttttcctca tcaaactctt cctcacgccc gtgttaatgc tcggcgtgtc catcgcggcg
     1381 aaaaagtggg gcacgttttg gggtggtttg atgtcgggtc tgccgctgac gtcaggggtg
     1441 attttgacgt ttttggcggt ggagcaaggt gcgggcttta ctcaaaccgc cattttgggt
     1501 gccattcctg gtttggcagc ggtgatggcg gcgtattgtg tgtatgccag cctcagcaag
     1561 cgccacgcat tgggtcttac gggcacggcg gctttgctca cgtttggctt gttgtcgtgg
     1621 gctttatcgc atttgcacag cgtgggatta agtgtcgccg tgttgttact gctgattttt
     1681 ttactgatac gcaaaacagg ccgtgaatac accccacaaa acctgatcat ccagcaaaac
     1741 ccttgggatg tgccgctgcg catgggtgcg tctactgctt tgctgttgct gattaccacc
     1801 gctgcgccgc atctgggttc aagtttaagc ggtgttttgt ccaccatacc cgtgattgca
     1861 tggccgctga tgctgtttac ccatgtgcaa tatggacgca atgaaatgct ggcggtggtg
     1921 cgcggcaatg ccatcggtgc attgagggta ttgagttttt atgcggtgat gtaccacagc
     1981 ttgcctgtgt tgcctttgtt gtgggtggtg gtattggcgg cgctggcatc ggtgatactg
     2041 cccttgttgt tgagcaaaac cttgccgcga aaatctgctt aagccatcca tctcatgcca
     2101 taaacaagcg gcctgtaaac cttgtcggct gtattcataa cacccgccat gcgcacagcg
     2161 tttacaggcc gcttcatcca ccttcaatga tgccatcatg ccaaccacac ctgcacgagt
     2221 cgcatcaaga ctcgccctca agccctaact cgttgtggca agaaccatcg ctcaagcttt
     2281 gcaaaatgcc gcaagtggcc atggttttca cgccgccaca tttttcacgc aaatccaata
     2341 aatgctgttt caaatgcaat aaggccacga tgcgttcttc cacgctgatg atgtgggcat
     2401 cgagcatggc attcacttcg gcacagtctt gctgcggtgc ggcgcgcaag cccaaaatgg
     2461 ttttgatttc ctccagcgac atatcaagcg aacggcaatg gcggatgaat tgcagccgct
     2521 ccaaatggcc ctcatcgtac aaacggtaat tgccgctgct gcgcagtggc tcaggcagca
     2581 aaccttcttt ttcgtaatag cgtatcgtca ccactttaca atcgctgcgc tttgccaatt
     2641 cgccaatttg cacttgcatt gcccactcct ttcaatgatt tacaggccgc ttgttgactc
     2701 tatagtcact ataggcattc taatgtcaaa taacaagttt tctgctgagg atgggtcatg
     2761 aagccacaca cacacacgca ttgttgcagc accgatcaca gccacgccga cttaccgcaa
     2821 atcgatttgc gtccaaacgc cgatgccgcc aagcgcgtgg cctaccgcat cgacaatatg
     2881 gattgcccca ctgaagaagc cttaatccgc aaaaaactcg cgagcatgga cggcatcgtt
     2941 gctttggatt tcaatttgat gcagcgggta ttgaatgtga gccacacctt gcccgacacc
     3001 cttgccatcg aacacgcctt agcgcaaatc gacatgcaag cggtcaagca aaccgccgcc
     3061 ctcaccacca gccgcatcgc cattcaagaa atggattgcc ctacggaaga aggcttaatc
     3121 cgcaaaaagc tcgcgggcat ggacggcata gacgatttgc aattcaattt gatggcgcgg
     3181 gtattgaccg tgagccacgc gcccgttgtc ttgccgaata ttgtggcggc tttgcagtca
     3241 ctcaccttca atcccaaagt gttggacagt gacaccgctg ccacggcatc agacacacca
     3301 gccccaagcc ctgagaaacc ttacaaaaaa tacctgttgg cggcggcatt ggtggcggcc
     3361 atcgctgctg aagcggtgaa ttttgccggc ggcagcacgg tattggaaat ggcgttggcg
     3421 gcatttgcca tcttggcaag cggcctgaaa acctaccaaa aaggctggat tgcgcttaag
     3481 aatttcaatt taaacatcaa tgccttgatg gcgattgcgg taacaggcgc ggtcatcatt
     3541 ggccattggc cagaagccgc gatggtgatg gtgctgttta ccatcgccga actgattgaa
     3601 gccaaatcgc tggaccgcgc acgcaatgcc atccgcggct tattggattt gagccccgaa
     3661 aaagccacgg tgttacaggc cgatggcact tggcaagaaa cattggcgag cgatgtggcc
     3721 gtgggcagca ccgtgcgcgt aaagccaggc gagcgtttag cattggatgg tgtgctcagc
     3781 aaaggcagct ccagcatcaa ccaagcgccc atcactggcg agagtctgcc cgtggaaaaa
     3841 gccgtcggcg atgcggtgtt tgcaggcacg attaacgaag caggctcgtt tgaataccaa
     3901 gtcaacgccg ctgccagcca ctctactttg gcgcgcatca ttgccgcggt ggaatcggcc
     3961 caaggcagcc gcgcgc
//
LOCUS       DAOMVK010000178         3975 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_6569_length_3975_cov_2.935459, whole genome shotgun sequence.
ACCESSION   DAOMVK010000178 DAOMVK010000000
VERSION     DAOMVK010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6569_length_3975_cov_2.935459"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..904
                     /locus_tag="PK856_06745"
     CDS             164..904
                     /locus_tag="PK856_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-specific adenosine deaminase"
                     /protein_id="HRL02103.1"
                     /translation="MQETESSTAVLSTPPLSPTVVRALNALNIHTLADVEALGAAHCF
                     ALLKASGLTITKSTLWQLGALVCHTTAQQLSPEQKQVLQAAYQALPPVHLPPHVEEQH
                     SYMQAALEQAQAAALQGEIPIGAVVVYQGQIIARAHNECVQQHFIGAHAEMLALQRAG
                     AVLQSYRLSECDVYVTVEPCMMCAGALLQARVKRVIYGIAEAKTGAIESQLQIATHNH
                     LNQHTAFFSGTNAEECRHLMQDFFKSKR"
     gene            1239..>3975
                     /gene="gltB"
                     /locus_tag="PK856_06750"
     CDS             1239..>3975
                     /gene="gltB"
                     /locus_tag="PK856_06750"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HRL02104.1"
                     /translation="MLHDRSLVKENCGFGLIAHLEGQPSHKVVRTAILGLSRMQHRGA
                     ILSDGKTGDGCGLLLQMPKSFFQIVAEENHVHLAKNFAVGMIFFPQDDALEAQYKAII
                     EEEFLQETLQVAWWRDVPVDASILGSIALESMPRIRQVFVNAPSGWREKDMERRMFIT
                     RRRIEKRIDHDDFYVCSLSNQVMIYKGLCMPKDLPKFYQDLGDLRMQSSICLFHQRFS
                     TNTLPKWKLAQPFRYLAHNGEINTISANRAWAQARAYKYRTPLIPDLQTAAPFVNNSG
                     SDSSSLDNMLELFVNGGMDLFRAMRLLVPPAWQNNPDMDADLRGFYDFNSMHMEPWDG
                     PAGIVLSDGRYAACTLDRNGLRPARYVITDDKLMTIASEVGIWDYAPDEVVCKGRVGP
                     GQLLVIDTRTGKLMHSHEIDNELKARHPYREWLDKNVLRLIPFEQMPDDEVGDAWLNQ
                     AELDVFQKQFGYNQEELESVLRILGENGQEAVGSMGDDAPFAVFSHKSRLVYDYFRQY
                     FAQVTNPPIDPLREAHVMSLATSIGREMSVFFEAEGMSSRMNFKSPVLMYSDMQQLLR
                     LPAKDYQHQVLKAVYNPNTTSLQDAIKRLCDEAVEAVKDGTVLLIVSDREIGKDLIQI
                     PAPLSVGAIQQRLVETNLRCDANIVVETGSARDPHHFAVLIGLGATAVYPYLAYESLA
                     NMVSRGAISKPLRVVTANFRNGINKGLYKIMSKMGISTISSYRCARLFELVGIGNEVA
                     DLCFKGMVSRINGAGFAELEGDLKRFHKTAWRKSQGLDAGGFLKYKPQGEYHMYNPDV
                     VNTLQQAVNSGNYQDYRAYADVVNNRPASTIRDLLRVKTEAATPIDVTQVEAAENLYT
                     RFDSAAMSIGALSPEAHEALATAMNRLGGYSNSGEGGEDPKRYGTEKVSRIKQVAS"
BASE COUNT         1057 a         1026 c          945 g          947 t
ORIGIN      
        1 aaagccttgg aattggtgaa atctcccgat gcttacaaaa cagcactggg caaaggtttg
       61 gacaaagcca aactggacgc agcgaaaaaa gacaaggaca aagccaaagc agatgaataa
      121 ggctgactct caaataactg cccgcactgc gggcagtttt tacatgcaag aaacagaatc
      181 aagcacggca gtcttgagca cgccgccatt gagtccaacg gtcgtgcgtg cattaaacgc
      241 attgaacata cacacactgg cggatgtaga agctttggga gcggcacatt gctttgcctt
      301 gctcaaagcc agtggcttaa ccatcaccaa atccaccttg tggcagcttg gagccttggt
      361 atgccacacc actgcacaac aattaagccc agagcaaaaa caggtgttac aggccgctta
      421 tcaagccttg ccacctgttc atttgccgcc ccatgttgaa gagcaacaca gctacatgca
      481 agcagcgctg gagcaggcac aggccgcggc attacaaggt gaaatcccca tcggtgcggt
      541 ggtggtttac caaggtcaaa tcattgcccg tgcccacaat gaatgtgtgc agcaacactt
      601 tatcggcgct catgcagaaa tgttggcctt acaacgcgca ggagcagtgt tacaaagcta
      661 ccgtctgtcg gaatgcgatg tttatgtcac ggtagaaccc tgtatgatgt gcgcaggggc
      721 tttgttgcaa gcccgagtca aacgggtgat ttatggcatt gccgaagcca aaacaggcgc
      781 gatagaaagc cagctccaaa tcgccaccca caatcattta aatcaacata ctgctttttt
      841 cagcggcacc aatgccgaag agtgccgcca tttaatgcag gatttcttta aatccaagcg
      901 ttaaatttta tgtaaatggc ttttttaaac aaaaatgcca atatattgcg catatcaagc
      961 gttttcgcct tttgttaagt tatttttaat ctcattattg gcataatcaa cgcaaaaccg
     1021 cgcttttgca cagcaacatc tgttaaaacc cgttaattca caaaaatgaa tatttttaaa
     1081 gatttagctt agacgtttga tagcactgtg cttttcttct caacttgagt acaaaaaaac
     1141 ctttgacgca gagcaacatt tttctgtatg tttacaaacc caaatgaaaa aataacctcc
     1201 tcatcgacac agaccagcgt cagaaaggtc ttaaagacat gttgcacgac cgctccttgg
     1261 taaaagagaa ctgtggcttc ggcttgattg cccacctaga aggccagccc agtcacaaag
     1321 ttgtccgtac cgctatcttg ggtttatccc gcatgcagca ccgtggtgcc atcctctcgg
     1381 atggaaaaac aggtgatggt tgtggcttac tcttacaaat gcccaaatcc ttcttccaaa
     1441 ttgtcgcaga agaaaatcat gtacatcttg caaaaaactt tgcagttggc atgatttttt
     1501 ttccccaaga cgatgcctta gaagcccaat ataaagccat cattgaagaa gaattcttgc
     1561 aagaaacctt gcaagtggct tggtggcgtg atgtgcctgt ggatgcgagc attctgggct
     1621 ccatcgcttt ggaatccatg ccgcgcatac gccaagtgtt tgtcaatgcc ccatcaggct
     1681 ggcgtgaaaa agacatggaa cgccgcatgt tcatcacccg ccgccgcatt gaaaagcgca
     1741 tcgaccacga cgatttttat gtgtgcagcc tgtccaacca agtgatgata tacaaaggct
     1801 tgtgcatgcc caaagacttg cctaagtttt accaagactt gggtgacttg cgcatgcaat
     1861 cgtctatttg cctgtttcac caacgcttct ctaccaatac cttgcccaaa tggaaactgg
     1921 cccagccttt ccgttacttg gcgcacaatg gcgaaatcaa caccatttct gccaaccgcg
     1981 cgtgggcaca agcgcgtgct tacaaatacc gcaccccatt gatacccgat ttgcaaaccg
     2041 cagcgccgtt tgtgaacaat tcaggctcgg attccagctc gctcgacaat atgttggaat
     2101 tgtttgtgaa tggtggcatg gacttgttcc gcgccatgcg tttgctcgtg ccacctgcat
     2161 ggcaaaacaa ccctgacatg gacgcagact tgcgcggctt ttacgatttc aactccatgc
     2221 acatggagcc ttgggatggc cctgcgggca tcgtgttgtc agacggtcgc tatgcggcct
     2281 gtacgctaga ccgcaatggc ttgcgccctg ctcgctatgt catcaccgac gacaaattaa
     2341 tgacgattgc ctcagaagtc ggcatttggg attacgcgcc tgatgaggtg gtgtgcaaag
     2401 gccgtgttgg ccctggtcaa ttgttggtca tcgacacccg cacgggcaaa ttgatgcact
     2461 cgcatgaaat cgacaacgag ctcaaagccc gccaccctta ccgcgagtgg ttggacaaaa
     2521 acgtgttgcg cttgattcca tttgagcaaa tgcctgacga tgaggtaggc gatgcttggc
     2581 tcaatcaagc cgaacttgat gtgttccaaa aacaatttgg ctacaaccaa gaagaattgg
     2641 aaagtgtgtt gcgcatcttg ggtgaaaacg gccaagaagc agtcggttcc atgggcgatg
     2701 atgcgccatt tgcggtgttt tcacacaaat cgcgcttggt atacgattat ttccgccaat
     2761 actttgccca agtcaccaac ccaccaatag acccattgcg tgaagcacac gtgatgagtt
     2821 tggccacctc cataggccgt gaaatgtcgg tgttttttga agccgaaggc atgtccagcc
     2881 gcatgaattt caaatcacct gtgttgatgt attccgacat gcagcaattg ctgcgcttgc
     2941 ccgccaaaga ctaccaacac caagtgttga aagccgttta caaccccaac accaccagct
     3001 tacaggacgc tattaaacgc ttgtgcgatg aagcagtgga agcggtaaaa gatggcacgg
     3061 tgttgttgat agtgtccgac cgtgaaattg gcaaagactt aatccaaata cctgcacctt
     3121 tgtctgtggg agcgattcaa cagcgcttgg ttgaaaccaa tttgcgctgc gatgccaaca
     3181 tcgtggtgga aacaggttcg gcgcgtgacc cacaccattt cgccgtgtta atcggcttgg
     3241 gtgcgaccgc ggtttaccct tatttggctt acgaatcttt ggccaatatg gtgtcgcgtg
     3301 gtgcgatatc caaacctttg cgcgtggtca ctgccaattt ccgcaatggc atcaacaaag
     3361 gcctgtacaa aatcatgtcg aaaatgggta tttccaccat ttcttcatac cgctgcgccc
     3421 gcttgtttga attggtgggc ataggcaatg aagtggccga cttgtgtttc aaaggcatgg
     3481 tcagccgcat caatggtgca ggctttgccg aattggaagg cgatttaaaa cgcttccaca
     3541 aaaccgcttg gcgcaagagc caaggcttgg atgcgggtgg cttcttgaaa tacaaaccgc
     3601 aaggcgagta ccacatgtac aaccctgatg tggtcaacac cttgcaacaa gcggtgaact
     3661 cgggcaatta ccaagactac cgcgcatacg ccgatgtggt gaacaaccgc cctgcttcca
     3721 ccatccgcga tttgctgcgc gtgaaaaccg aagccgccac accaatagac gtgacccaag
     3781 tcgaagccgc cgaaaacctc tacacccgct ttgactcggc tgccatgtcc ataggcgcac
     3841 tcagccctga ggcacatgaa gccttggcaa ccgcgatgaa ccgtttgggt ggctattcca
     3901 attcaggcga aggcggcgaa gacccgaaac gctatggcac cgaaaaagta tcgcgcatca
     3961 agcaagtggc ttcag
//
LOCUS       DAOMVK010000179         3960 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_6607_length_3960_cov_2.795391, whole genome shotgun sequence.
ACCESSION   DAOMVK010000179 DAOMVK010000000
VERSION     DAOMVK010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6607_length_3960_cov_2.795391"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1910)
                     /gene="pgaB"
                     /locus_tag="PK856_06755"
     CDS             complement(<1..1910)
                     /gene="pgaB"
                     /locus_tag="PK856_06755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HRL02105.1"
                     /translation="MTLSLWTRFWQAVLLCLIALSAPLALAKNNQYGTLAYHDVVDEA
                     GPLYHQVKTDVKSPKSEILKAYYPQTISKKTLISHFNWLKNNGYNVVGWQEVIDAQAG
                     KKPLPDKAVLLTFDDGYESFYRIVFPLLKEYNYKAVFAVVTSLVDQAPNSMVVFGDSR
                     IARGAFVTWPQIKEMSDSGLVEIASHTHNMHRAIKANPGGTAYPASFSAQYANGKYET
                     PAEYHQRLQADYKLSGERIQQHTGKFPDVLVWPYGQFNDHAWRVANELGYKHHFTLHD
                     ERVNPKLTHDEVGRFLIEEETLLETLSEYFGGRLRNKSLERVMHVDLDYVYDTDPKQM
                     SKNIDALIERVYQSGATTVYLQAYADEDGNGVAEKLYFPNRHLPMKKDLFGQIAWQLM
                     TRANVKVFAWMPVMAFDLGPNADYVKDTRLNKVNPEHYLRLNPYNPANIKIINEIYAD
                     LSFYSKFNGLLFHDDAFLTDYEGPISGKRDDATLDQEAEDKTQYLIQLTKGLEKTVSE
                     YSYRGNAKLKSARNIYAEVVINPKAKQWFAQDYQAFLEHYDRTAIMAMPYMEAQHAID
                     AKTAKQWLNDLVKKTDLVKNNDKVVFELQAQNWKTKKAIPEAELNQWFDLLRNQGVYH
                     YGYYPDDLVMKKP"
     gene            complement(1922..>3960)
                     /gene="pgaA"
                     /locus_tag="PK856_06760"
     CDS             complement(1922..>3960)
                     /gene="pgaA"
                     /locus_tag="PK856_06760"
                     /inference="COORDINATES: protein motif:HMM:TIGR03939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HRL02106.1"
                     /translation="SKEGNVQKDGQLGLWLTSIDQRQQEMAIVAGQEYAQRFGADASA
                     QDAQGYYAQVFGQAWPTNSANAGNDSTEQSQKANVQAEFRTAAKQKRFAKQRELIRQY
                     PQWFSTNDATWLDVGEQQEIIATTQASTIDNTRALQTANAQLEQVLAAHPDNIELQKT
                     GLPSILVAAVRLSEPKPGIVAYQKLQAIEPNIPAETKALYADALLTNKQPHQALKIMS
                     SIPKEQLTDEMQDRVIAAYADMGYMSKAQAARKNWNITPKRNDFTHNYRVSNPYATER
                     MFWDIRLLDWDGKHRLANQMVQERLQNAPADTSALRLKGDLRRWQGFPDDALAAYDEA
                     AYYIHPDERVGLEVNRATVYLDQGDYRKAHASIDKLPDYSSSKQDLVKRLDLQAAPQL
                     NVQTAWSDTTSPPQQQHEWSINSQLFSKRSHDGHRAYVEHKVETSPFSNESLRMQRVG
                     VGAELNFYPTQIDVAVGHGTELNNKPYLHAGVNHTFNQWFKAGLELQKNSESTPLRAL
                     EQGVYADAVIANISMHLAPDVDAGLGFSLNDFDDGNLRREAYAYVSSTLWQRDQFSLK
                     NYSRVDWQKNRNTPSAAYYNPEQAHSFSTEMTAQYRHLFDHDIQLTQQLTAGIGRYYQ
                     KNQPAEDTWVVRYGHSWDVQDAYSVGYSVGRKRSVYDGNPEYSNFIGLNASIKF"
BASE COUNT          946 a          872 c          949 g         1193 t
ORIGIN      
        1 ggttttttca tcaccaaatc atcggggtaa tagccataat gatagacacc ttggttgcgc
       61 aacaagtcaa accattgatt cagctctgct tcaggtatgg cttttttggt tttccaattt
      121 tgcgcttgta attcaaacac cactttgtca ttgtttttga ccaggtcggt tttttttacc
      181 aagtcgttca accattgttt cgctgttttg gcatcgatgg catgctgtgc ctccatgtaa
      241 ggcatggcca tgatggctgt gcgatcgtaa tgctctaaaa aagcttggta gtcttgtgca
      301 aaccattgtt tggctttggg attgatgacc acttctgcat aaatgttgcg ggcacttttt
      361 aatttggcat taccacggta gctgtattca gaaactgttt tttctaagcc tttggtcaat
      421 tgaatcaaat attgggtctt gtcttcagct tcttgatcta aggtggcatc atcgcgtttg
      481 ccactgatag ggccttcata gtcggttaag aatgcatcat catgaaagag caagccattg
      541 aatttgctgt aaaagcttaa gtctgcataa atctcattaa tgattttaat attggccggg
      601 ttgtaaggat tcaagcgcaa ataatgctct ggattgactt tgttcaatcg agtgtctttc
      661 acataatcgg cgttgggtcc caaatcaaaa gccatcacag gcatccaagc aaataccttc
      721 acatttgccc gagtcattaa ttgccatgca atttgcccaa acaaatcttt tttcatgggt
      781 aaatggcggt taggaaaata cagtttttcc gcaacaccat tgccatcttc atcggcatag
      841 gcttgtaaat atacagtggt cgcgcctgat tgatacactc gctcaatcaa ggcatcaata
      901 tttttggaca tttgtttggg atcggtgtcg tatacataat ccaaatccac atgcatcacg
      961 cgttctaggc ttttattgcg caagcggccg ccaaaatact cagacagtgt ttctaacaag
     1021 gtttcttctt ctatcaagaa acgacctact tcatcgtgag tcagttttgg gttgacgcgt
     1081 tcgtcgtgta aggtgaaatg gtgtttgtag cccaactcat tggcaacacg ccaagcatgg
     1141 tcattaaatt gaccgtaagg ccacaccaat acatctggga acttacctgt gtgttgctgt
     1201 atgcgttcac cagaaagctt atagtcggcc tgtaagcgtt ggtggtattc tgcgggcgtc
     1261 tcgtatttgc cattggcgta ttgggctgaa aatgatgctg gataggcagt gccaccagga
     1321 ttggctttga tggcgcggtg catattgtgg gtatgtgagg caatctcaac cagaccactg
     1381 tcactcatct ctttgatttg tggccaggtg acaaatgcgc cacgggcgat gcggctgtca
     1441 ccaaacacca ccatgctgtt aggcgcttgg tctaccaaag aggtcaccac tgcaaacacg
     1501 gctttgtagt tgtattcttt caataatggg aaaacaatgc gataaaagct ttcatagcca
     1561 tcatcaaagg tgagtaaaac cgctttatca ggtaagggtt tcttacctgc ttgtgcatct
     1621 atgacctctt gccaacccac cacgttatag ccattgtttt ttaaccagtt aaagtgggaa
     1681 atcaaggttt ttttggaaat ggtttgtggg tagtaagcct tcaggatttc gcttttgggg
     1741 cttttcacat cggttttgac ctgatggtag agggggccag cctcatccac cacatcgtga
     1801 taggccaagg ttccgtattg gttgtttttg gccaaggcaa gaggtgctga taaggcgatg
     1861 aggcacagta agactgcttg ccaaaaacga gtccatagag aaagagtcat gtattgcctc
     1921 actaaaattt aatgctggcg ttgaggccaa taaaattgct gtattcaggg ttgccatcgt
     1981 aaacagaacg ttttctgcct acggaatagc ccacactgta ggcatcttgc acatcccatg
     2041 aatggccata tcgcacgacc catgtgtctt cggcaggctg atttttttga taatatcgac
     2101 caatgccagc ggttaactgt tgggtaagct ggatgtcgtg atcaaaaagg tggcggtatt
     2161 gagccgtcat ctcagtcgaa aaagagtggg cttgttcagg gttgtaatag gctgcagaag
     2221 gggtattgcg gtttttttgc cagtcaacgc ggctgtagtt ctttaagctg aactgatctc
     2281 gttgccacaa tgtgctgctt acataggcat aagcttcacg gcgcaaattg ccatcgtcga
     2341 aatcattgag tgagaaaccc aggcctgcat ccacatcagg tgctaaatgc atgctgatgt
     2401 tggcaatgac cgcatcggca taaacgcctt gttccaatgc tcgcagtggt gtggattcac
     2461 tgtttttttg taattccaaa cctgctttga accattgatt gaaggtgtgg ttgactccgg
     2521 catgtaagta aggcttgtta ttcaactcgg taccatgacc gacagccaca tcaatttggg
     2581 ttgggtaaaa gtttaattct gcacccactc ccacccgttg catgcgtaaa gactcattgg
     2641 agaatggcga tgtttcgacc ttgtgttcca cgtaagcgcg gtgaccatca tgtgagcgtt
     2701 tgctgaacaa ttggctattg atgctccact catgctgttg ttgagggggt gaggtggtgt
     2761 ctgaccaagc agtttgtaca ttcaactgag gagcggcctg taagtccaaa cgtttgacca
     2821 aatcttgttt actgctggaa taatcaggca gcttatcaat actggcatgg gctttacggt
     2881 aatctccctg atccaaatac accgtggcac ggttgacttc caatcccact cgctcatcag
     2941 ggtggatgta ataagccgct tcatcatagg cagccaaagc atcgtctgga aaaccttgcc
     3001 agcggcgcaa atcacctttc aaacgtaagg cgctggtgtc agctggcgca ttttgtaagc
     3061 gttcttgtac catttgattg gccagtcggt gtttgccatc ccaatcaagc aaacgaatgt
     3121 cccaaaacat gcgttcagtg gcgtaaggat tggaaacccg gtaattgtgg gtaaaatcat
     3181 tgcgtttggg ggtgatgttc cagttttttc gggccgcttg ggccttactc atgtaaccca
     3241 tgtccgcata agccgcaatc actcggtctt gcatctcatc ggttaattgc tctttcggga
     3301 tgctggacat tatttttaaa gcttggtgag gctgcttatt ggtcaataag gcatcggcat
     3361 acaaagcctt ggtttcagcc ggaatattgg gttcaatcgc ttgtaacttt tggtaagcaa
     3421 cgatgcctgg ttttggctca ctcaagcgca cagcggcaac caaaattgac ggcagacctg
     3481 tcttttgcaa ctcgatattg tcaggatgtg cggccagaac ttgctctaat tgggcattgg
     3541 cagtttgtaa agcacgggta ttgtcgattg ttgaggcttg ggtggtggcg atgatttctt
     3601 gttgttcacc tacatccagc catgtggcat cgttggtgct gaaccactgc gggtattgac
     3661 ggattaattc acgttgtttg gcaaagcgct tttgtttggc ggcagtgcga aattctgctt
     3721 gtacgtttgc tttttgactt tgctctgtac tgtcattgcc tgcgttagcg gaatttgttg
     3781 gccatgcttg accaaaaacc tgcgcataat aaccctgtgc gtcttgcgct gaggcatccg
     3841 caccaaaacg ttgcgcatat tcctgacctg caacaatcgc catttcttgt tgtcgttgat
     3901 ctatcgaagt cagccacaac cccaactgcc catctttttg tacattgcct tctttgctgt
//
LOCUS       DAOMVK010000180         3952 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_6627_length_3952_cov_3.013600, whole genome shotgun sequence.
ACCESSION   DAOMVK010000180 DAOMVK010000000
VERSION     DAOMVK010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6627_length_3952_cov_3.013600"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..1459)
                     /gene="gabT"
                     /locus_tag="PK856_06765"
     CDS             complement(173..1459)
                     /gene="gabT"
                     /locus_tag="PK856_06765"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HRL02107.1"
                     /translation="MSKVEITQRRLAATARGVGIMCDFYIDKAKNAELWDTEGNRYID
                     FAGGIGVLNTGHLHPKVQAAVAEQLTKFSHTCYQVVPYESYTEAAERINAKAPVSGTA
                     KTFFLTTGAEAVENAIKVARSYTGRPGVISFQSGYHGRTNMTLGMTGKVAPYKLGFGP
                     FPADIVHAQYPNELYGVSSEEALASLQRQFKTTIDPKEVAAIIIEPIQGEGGFNVTPK
                     AFLQGLRDICDEHGIVLIFDEVQTGFARTGTLFATEQMGVKPDLITMAKSLAGGFPIS
                     GLVGKAEIIDAPAAGGLGGTYAGSPLALAAVLAVLDVIEEEKLCERSQQLGDKLRARL
                     EGLKDKIPQIRMVRGLGGMIAVEFMKPGTDEPDPEFTKRAQTHALENGLILLTCGMYY
                     NVMRFLFPVTIEDEIFEESLGILEAALTHAAATAEA"
     gene            complement(1758..2285)
                     /locus_tag="PK856_06770"
     CDS             complement(1758..2285)
                     /locus_tag="PK856_06770"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HRL02108.1"
                     /translation="MNWNTSSLLCAMAMPWIQATKVFLNQVAALKIPMFGICFGHQLM
                     AEAFGGKVEKSDKGWGVGIDSYQMGNVEQDVLVYHQDQVVRLPESAKVRAHSDHCEYG
                     ALEYDFPALSVQCHPEFTQGLVLDLLERGRSEFGDDKTNRAESQTEQAQLHNQQFAQH
                     VVTFFQQHQRVNSEA"
     gene            complement(2600..3799)
                     /locus_tag="PK856_06775"
     CDS             complement(2600..3799)
                     /locus_tag="PK856_06775"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009119952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRL02109.1"
                     /translation="MTQFRDAYIVAATRTPVGKAPRGMLRTTRPDDLLAHVIKSVVGQ
                     VPEFDLRDINDVVAGCAFPEAEQGLNMARISVLLAGLPNTVGGITINRYCSSGINALQ
                     IAADRVRTGEADMTIAAGVESMSMIPMMGNKVALNPAIFENDEHLAIAYGMGLTAENV
                     ANQWKVSREDQDAFAVESHRRALAAIAAGKFKAEISPIEAIYRSADLKTREALEIRKW
                     LDTDEGPRADTTLEGLAKLRPVFAAKGSVTAGNSSQMSDGAGALLVCSEEAIKRYGFT
                     PLARYDGFAVRGVPPEIMGIGPKEAIPAVLKSSGRTLADMKWIELNEAFAAQALAVIR
                     DLNLDTNIVNPNGGAIALGHPLGATGAIRAATVIHGMRERGETGYGMVTMCIGTGMGA
                     AGLIEVL"
BASE COUNT         1014 a         1038 c          880 g         1020 t
ORIGIN      
        1 cattggcagg attggtcacg tcaaccgtgg caccgttatc agcattcaac catgtgccat
       61 caatgtaaca ttgggttttg aacaaagcgt tgtcttgtaa ttgcatttta attccttcat
      121 gtcctcgaaa taaaagcgcc ctgagtttac aggacgcctt tttaaaagtg tcttatgctt
      181 ctgcagtggc tgctgcgtgg gttaacgccg cttctaagat gcccaaagac tcttcgaaga
      241 tttcatcttc gatggtcact gggaacaaga aacgcatcac gttgtaatac atgccgcaag
      301 tgagcaaaat caagccattt tccaaggcgt gggtttgggc acgtttggtg aattctggat
      361 caggttcatc tgtgcctggt ttcatgaatt caaccgcaat catgccgccc aaaccgcgca
      421 ccatgcggat ttgtgggatt ttgtctttca agccttccaa acgagcgcgc aatttgtcgc
      481 ccaattgttg tgaacgctcg cacagttttt cttcttcaat gacatccaat accgccaata
      541 ccgcggccaa tgccaatggg ctacctgcat aagtgccgcc caaaccaccc gctgctggtg
      601 catcgatgat ttcagcctta cccaccaagc cagaaatagg gaaaccaccc gccaatgatt
      661 tggccatggt aatcaaatca ggtttcacgc ccatttgctc agtggcaaac aaagtacctg
      721 tacgcgcaaa gcctgtttgc acttcatcaa aaatcaacac gatgccgtgt tcatcacaaa
      781 tgtcgcgcaa gccttgcaag aacgctttag gggtcacatt gaaaccgccc tcaccttgaa
      841 tcggctcgat gatgatggca gccacttctt taggatcgat ggtggttttg aattggcgtt
      901 gcaagctggc caaagcctct tctgagctca cgccatacaa ttcatttggg tattgtgcgt
      961 gcacgatgtc ggcagggaaa ggaccaaaac ccaatttgta aggggcaacc ttacctgtca
     1021 tgcccaaagt catgttggta cgaccgtggt aaccgctttg gaatgaaatc acaccagggc
     1081 ggcctgtata agaacgggcc actttaattg cgttctctac ggcttcagca cccgtggtca
     1141 agaaaaaggt tttcgcagta ccgctcacag gcgctttggc attgatgcgc tcagccgctt
     1201 cggtatagct ttcgtaagga accacttggt agcaagtgtg gctgaatttg gtcaattgtt
     1261 cggccacagc ggcctgtact ttaggatgca aatgacctgt gttcaacaca ccaataccgc
     1321 ccgcgaaatc aatgtaacgg ttgccttcgg tgtcccaaag ttcggcattt ttcgctttgt
     1381 cgatataaaa atcgcacatg atgcccacgc cacgagcggt ggctgccaaa cggcgttggg
     1441 tgatttctac tttactcatt ttgtctccta actgacttat ataatgtgtc gttttagatt
     1501 tggcagcaca cacctgaatg gtatttgggt tgcacacgca acgaccaacc gactgacaaa
     1561 gagttgtgtt tgcttatttt cgatatcctc agagcctctc atacccgctg caagaaatca
     1621 atatgcgcct taaaataaat ttcactgagc aaacccatgt ttcgcagagt gaaacttgag
     1681 actttattgc ttagtagcaa caacacaagc ttcttctgcc aacaagacgg atttgcctca
     1741 ataacgatta ttgctgatta cgcttccgaa tttacgcgct gatgctgctg aaaaaaagtc
     1801 accacgtgct gggcaaactg ttgattgtgc agttgcgcct gttcggtttg gctttcagca
     1861 cgattggttt tatcatcacc aaattcagaa cgcccgcgtt ccaacaaatc caataccaag
     1921 ccttgggtaa actcagggtg acactgtacc gacaatgcgg ggaaatcata ttccagcgca
     1981 ccatattcac agtgatcgga atgcgcccgt actttcgcac tttcaggcaa gcgcaccact
     2041 tggtcttggt gatacaccaa tacgtcctgt tcgacattac ccatttgata agagtctatg
     2101 cccacgcccc aacctttgtc cgatttttca actttgccgc caaaggcttc ggccatcaat
     2161 tggtgaccaa agcaaatgcc aaacatcgga atcttcaagg ctgccacttg gttcaaaaac
     2221 actttggtgg cctgtatcca aggcatcgcc attgcacaca ataaactgct ggtattccaa
     2281 ttcaacctct tgtccactgt tgcccaacca agtgcgtatc aattcaaaaa aactgccgtg
     2341 tttttgtgca aggttttctg gcaacaagcc catttctaag attgcgattt tcattgttaa
     2401 acaagtccgt atgtgaattg aatcatgtgt tcggctgatt caaaccgccg ctttatacag
     2461 atgaaatata gcattaaaaa acaattcatt acaaacaagc taaccgatac ttacactctc
     2521 tgctatcaga gcaaatagcc tgatacagca agacaaaaaa aagccctctt gcaagcaaga
     2581 gggcttaaaa ggacagcaat tacaatactt cgatcaaacc agcagcaccc atgcccgtac
     2641 caatacacat ggtcaccatg ccataacctg tttcaccgcg ctcgcgcata ccgtgtatca
     2701 cagtggctgc acgtatcgca ccggttgctc ccaatgggtg gcccaaagca atcgctccgc
     2761 catttggatt aacaatattg gtgtccaaat tcaaatcacg aatcacggcc aaggcttgtg
     2821 cagcaaaggc ttcgttcaat tctatccact tcatgtctgc caacgtgcgg cctgaagatt
     2881 tcaataccgc aggaatggct tctttcgggc ctatgcccat gatttctggt ggcacaccgc
     2941 gcactgcgaa accatcgtaa cgtgccaatg gcgtaaagcc ataacgctta atggcttctt
     3001 cagaacacac caacaaagca cccgcaccgt cagacatttg cgatgagtta cccgctgtta
     3061 ctgagccttt ggcggcaaac acagggcgca atttggccaa gccttccaaa gtggtgtcgg
     3121 cgcgtgggcc ttcatcggtg tccaaccact tgcgaatttc caaagcctcg cgggttttca
     3181 aatcggcgct gcggtaaatg gcttctatcg ggctgatttc ggctttgaat ttgccagcag
     3241 ctatcgcggc caaagcacgg cggtggcttt ctacggcaaa cgcatcttgg tcttcgcgcg
     3301 ataccttcca ttggttggcc acgttttcag cggtcaaacc catgccgtaa gcaatggcaa
     3361 ggtgttcatc gttttcaaaa atcgccggat tcaaggccac tttattgccc atcatcggaa
     3421 tcatagacat cgattccacg cctgcggcga tggtcatgtc ggcttcgcct gtgcgcacac
     3481 ggtcggccgc gatttgcaag gcattgatgc ccgatgagca atagcggttg atggtgatgc
     3541 caccaactgt gttaggcaaa cccgcgagca acacgctgat gcgcgccatg ttcaagcctt
     3601 gttcggcttc aggaaaagca caacctgcca ccacatcgtt gatgtcgcgc aaatcaaatt
     3661 cgggcacttg gccaaccacg cttttaatca cgtgcgccaa caaatcatca ggacgggtgg
     3721 tacgcaacat accgcgcggg gctttgccaa ctggggtacg ggttgctgca acaatataag
     3781 catctctaaa ttgagtcatt tatttctcca aattggggcg acaagcggcc tgtaaacggt
     3841 tacaggccgc ttttaggcat cagttacgca atggtttacc ggtagacatc atgttttcga
     3901 tgcgctctaa ggttttctct tgttgcaaca aagaaataaa cactttgcgc tc
//
LOCUS       DAOMVK010000181         3941 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_6656_length_3941_cov_3.151312, whole genome shotgun sequence.
ACCESSION   DAOMVK010000181 DAOMVK010000000
VERSION     DAOMVK010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6656_length_3941_cov_3.151312"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2237
                     /locus_tag="PK856_06780"
     CDS             <1..2237
                     /locus_tag="PK856_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HRL02110.1"
                     /translation="IPVRQRDLQQARQRLHRIRADKVILAAGAIERPLVFGNNDMPGV
                     FTVSAAQTYLNRYGVLLGEKIAICGNNDEIYMAARDLALAGAEVVVADVRKNSKVPSF
                     LPRQVKVHLHSGIAQVMGTGEVSAVEIIDLNTLAKQTWQCEIVLSSGGLSPTVHLYCH
                     DTSRPIWDAAAMAFIVPKQYAACKERVCNVGAVTGSKTWQQALEQTQAVLVEFLGSKG
                     LQAAIELPQVREEAFQAMELAVRIPDGLPAGKGAKAFVDYQNDVTAEDIKITIQENYR
                     HVEHVKRYTAFGFGTDQGKLSSVNSFILTAEAMGAPIEQVSTTTYRPAYTPVSLGVLA
                     GLDADNTFDPERVTGIHQCHQSRGAAFEPVGQWLRPWYFAKAGEDMHAAVNRECLAAR
                     NGVGIMDASTLGKIQIDGPDAREFLNRIYSNAWSKLEPGKCRYGLMLNENGMVMDDGV
                     TACINDEQFYMTTTTGGAANVLSWLERWHQTEWPELKVWMTSVTDHWGTIAVVGPKAR
                     LVLQKLCDDIDFSAEAFPFMDWKAGTVAGLPARVFRVSFSGELAYEINVEAGYGHYVW
                     EQVMAAGAEFDITPYGTETMHVLRAEKGFIIVGQDTDGSMTPIDLGMAWAVGMKKPFS
                     FLGKRSLSRSDTARDNRKHLVGLLPVDSKAVIPEGAQIIASANVQVPKAGGYDAPVPL
                     LGHTTSSYYSAFLGRSIAMAVIENGRNRIGETLYAYSRGNFIEFKVADSTVFVDAKGE
                     RQHA"
     gene            2230..2874
                     /locus_tag="PK856_06785"
     CDS             2230..2874
                     /locus_tag="PK856_06785"
                     /inference="COORDINATES: protein motif:HMM:NF016180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HRL02111.1"
                     /translation="MLNSTTQAYWHTPLHYQWAAVPEQTGHAALQLSSGGAVGIINLR
                     GLLGDAAFVAAIDSALGVALPTAPKQSVYAQDAAIFWLSPDEWLLVCAYAKKNEVLAR
                     LQAACKGVFAQVVDNSGGFMAVQIQGSEAETFLRHLSPYNVSALQDGQVVSTVLKKTT
                     VVIHKVGANDYVLLFRRSFADYLWRIFEKTAKPYGMGVSQNWQSRQADWQRYTA"
     gene            3062..>3941
                     /locus_tag="PK856_06790"
     CDS             3062..>3941
                     /locus_tag="PK856_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HRL02112.1"
                     /translation="MSKLHQQRHKTQKIGFILLRGFTMITFASAVDVLRMSNYHSQNE
                     LYSWHIYQESDDICASNGLRLEHTCDAAGLFECDMVFVCAGIDVQTATTPTIKQLLQQ
                     LDQQGITLGGMCNGSVSLALAKLMDGYRSAVHWESLPAAKEAFLQVKFGEQLYALDRN
                     RYTCSGGTASMDMMLHIIRLQHGKALATAVSQQFVMERIRDDSTTQYKPHTEHIGPGY
                     DYVHTAIELMQANLDEVLAMQEIADLIPLSLRQMERLFKRYCNMSPAQYYLRLRLQRA
                     KELLAQTSMSVMQVTVA"
BASE COUNT          966 a          965 c         1081 g          929 t
ORIGIN      
        1 acattcctgt gcgccaacgc gatttgcaac aagcgcgtca acgcttgcac cgcatacgtg
       61 ccgacaaggt gattttggcc gcgggtgcga ttgaacgccc attggtgttt ggcaacaacg
      121 acatgcctgg cgtgttcact gtgtctgccg cccaaaccta tttaaaccgc tacggcgtgt
      181 tattgggcga aaaaatcgcc atttgtggca acaacgatga gatttacatg gcagcgcgcg
      241 atttggcctt ggctggcgcg gaagtcgtgg tggccgatgt gcgcaaaaac agcaaagtac
      301 cgagtttttt gccacgtcaa gtcaaagtgc atttgcacag tggcattgcc caagtgatgg
      361 gcacaggcga agtgagtgcg gtagaaatca tcgatttgaa cacactggcc aaacaaacat
      421 ggcagtgtga aattgtgttg tccagcggtg gcttgtcgcc caccgtacac ctgtattgcc
      481 acgacaccag ccgcccgatt tgggatgctg cggccatggc cttcatcgtg cccaagcaat
      541 atgcggcctg taaagaacgc gtgtgcaatg tgggcgcggt aaccggcagc aaaacttggc
      601 aacaggcttt ggaacaaacc caagcggtgt tggtggaatt cttgggcagc aaaggtttac
      661 aggccgctat tgagttgcca caagtgcgcg aagaagcctt ccaagcgatg gaattggcag
      721 tgcgcatacc cgatggtttg cctgctggca aaggcgcgaa ggcgtttgtt gattaccaaa
      781 acgacgtgac ggcagaagac atcaaaatca ccattcaaga aaactaccgc catgtggagc
      841 acgtgaagcg ctataccgca tttggttttg gtaccgacca aggcaagcta tcgagcgtga
      901 acagctttat cttaaccgct gaagccatgg gcgcgccgat tgagcaagtg tctaccacca
      961 cttacaggcc ggcttacacg ccggtgagct tgggcgtgtt ggcaggtttg gatgcagaca
     1021 acaccttcga tccagaacgc gtgacgggca tacaccaatg tcaccaaagc cgtggcgctg
     1081 catttgagcc cgtgggtcaa tggttgcgtc cttggtattt tgccaaagct ggcgaagaca
     1141 tgcatgcggc agtaaaccgc gaatgtttgg ccgcacgcaa tggagtgggc atcatggatg
     1201 cgtctacctt gggcaaaatc caaatcgatg gccctgatgc acgtgaattc ttaaaccgca
     1261 tttacagcaa tgcgtggagc aaacttgagc cgggcaaatg ccgctatggc ttgatgttga
     1321 acgaaaacgg catggtgatg gacgacggtg tgaccgcatg catcaacgac gaacaattct
     1381 acatgaccac caccacaggc ggcgctgcca acgtgttgag ctggctggag cgttggcacc
     1441 aaaccgaatg gcctgaattg aaggtgtgga tgacatcggt aaccgaccat tggggtacca
     1501 tcgccgtggt aggcccgaaa gcacgactgg tattgcaaaa actgtgtgac gacattgatt
     1561 tttcagccga agccttccca ttcatggatt ggaaagccgg cacggtagcg ggtttgcctg
     1621 cacgcgtgtt ccgcgtgtct ttctcgggtg aattggcgta tgaaatcaat gtggaagcag
     1681 gttatggcca ctatgtgtgg gaacaggtaa tggcggcggg tgctgagttt gacatcaccc
     1741 cttacggcac cgaaaccatg cacgtgttgc gcgctgaaaa aggcttcatc attgtgggcc
     1801 aagacaccga tggctcaatg acgccgattg acttgggcat ggcttgggct gtgggcatga
     1861 agaaaccgtt ctccttcttg ggcaaacgct cgctttcacg cagcgacacc gctcgcgaca
     1921 accgcaagca cttagtgggc ttgttgccag tagactcgaa agcagtaata ccagaagggg
     1981 cgcaaatcat tgccagcgcc aatgtgcaag tgcctaaagc aggcggctac gatgcacctg
     2041 tgcctttgct gggccacacc acctcgagtt attacagtgc cttcttgggc cgatcgattg
     2101 cgatggcggt gattgaaaat ggtcgcaacc gcatcggtga aaccttatac gcttattctc
     2161 gtggcaattt cattgaattt aaagtggccg acagcaccgt atttgtcgat gccaaaggag
     2221 aacgtcaaca tgcttaattc gactacccaa gcttactggc acacgccttt gcattaccaa
     2281 tgggccgctg tgcctgaaca aaccggtcat gccgctttgc aattgagcag cggcggtgcg
     2341 gtgggcatca tcaatttgcg tggcttgctc ggcgatgcgg cttttgtggc ggcaatagac
     2401 agcgctttgg gtgtggcttt gcccacagcg cccaagcaaa gtgtgtacgc acaagacgct
     2461 gcgatatttt ggttgtcacc cgatgagtgg ttgttggtgt gcgcctatgc caagaaaaac
     2521 gaggtattgg cgcgtttaca ggccgcttgt aaaggggtgt ttgcccaagt ggtggacaac
     2581 agtggtggct ttatggccgt gcaaattcaa ggctctgaag cggaaacatt cttgcgccat
     2641 ttgagccctt acaatgtatc ggctttacaa gatggccaag tggtgtctac ggttttgaaa
     2701 aagaccacgg tggtaattca caaagtgggc gcaaacgatt atgtgttgct gttccgtcgc
     2761 agcttcgccg attatttgtg gcgcattttt gaaaaaaccg ccaagcctta tggcatgggt
     2821 gtgagccaaa actggcaaag ccgccaagca gattggcagc gttacaccgc gtagtttccc
     2881 ttcgttcttc ttatccttca gtctcatctt cgcctctcat gcagtgaacc atgagaggca
     2941 tttttttcaa aataatgtcg taatttaacc attaattgtc gataatgtgc ggtaatgaaa
     3001 tggcaatgtc agtcacctta agcattaaaa ataacagcaa atacagagaa atagaggcca
     3061 tatgagtaaa ttacaccagc aacggcataa aacccaaaaa attggcttta tcttattgcg
     3121 tgggtttacc atgattacct tcgccagcgc cgtcgatgtg ctgcgcatga gcaattacca
     3181 cagtcaaaac gaattgtatt cttggcatat ctaccaagaa agcgacgaca tttgcgccag
     3241 taatggtttg cgtttggaac atacttgcga tgctgcgggc ttgtttgaat gcgacatggt
     3301 gtttgtgtgc gcaggcatag acgtgcaaac cgccaccacg cctactatta agcaattgtt
     3361 acaacagcta gaccaacaag gcatcacctt gggtggcatg tgcaatggtt cggtgtcttt
     3421 ggccttggcc aaactgatgg atggctaccg cagcgccgtg cattgggaga gtttgcctgc
     3481 ggccaaagag gcttttttac aggttaaatt tggcgagcaa ttgtatgcac tagaccgcaa
     3541 tcgctatacc tgctcgggcg gcacggcttc tatggacatg atgctgcaca tcattcgttt
     3601 gcaacacggc aaggccttgg ccaccgcggt gtcgcaacaa tttgtgatgg agcgcatccg
     3661 cgacgattcc accacccaat acaagcccca taccgaacac ataggcccag gctacgatta
     3721 tgtgcacacc gcgattgaat tgatgcaggc gaatctggat gaggttttgg ccatgcagga
     3781 aattgccgac ttgataccgc tgtctttgcg gcagatggag cgcttgttca agcgctattg
     3841 caatatgtca cctgcgcaat actatctgcg cttgcgcttg caaagagcca aagaattgct
     3901 ggcgcaaacc agcatgagcg tgatgcaagt gaccgtggct t
//
LOCUS       DAOMVK010000182         3933 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_6674_length_3933_cov_2.632800, whole genome shotgun sequence.
ACCESSION   DAOMVK010000182 DAOMVK010000000
VERSION     DAOMVK010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6674_length_3933_cov_2.632800"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="PK856_06795"
     CDS             <1..824
                     /locus_tag="PK856_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRL02113.1"
                     /translation="VQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKADDEWL
                     LLDVTPLSLGLETYGGLVEKVIPRNSTIPTAMAQEFTTFKDGQTAMAIHVVQGERELV
                     SDCRSLARFTLRGIPPMVAGAARIRVTFQVDADGLLSVSAQEQTTGVQAAIEVKPSYG
                     LDDTTITQMLKDSMQFAQEDMAQRARQEALVEAESIVTAVSTALMSDGDLLTESERSD
                     IEVVIKRLQLQMAQGDAASIRASVEALNHATDAFAAARMDRNIQRALTGQNIADI"
     gene            842..1078
                     /locus_tag="PK856_06800"
     CDS             842..1078
                     /locus_tag="PK856_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRL02114.1"
                     /translation="MSFSPEQISELLQLPFVGKTVIQRLQEVGLDDVHALANSNATEV
                     LEVIAAHLHSSCWKNSPQAKQSIHNIIAWAKQYP"
     gene            1087..1425
                     /gene="fdx"
                     /locus_tag="PK856_06805"
     CDS             1087..1425
                     /gene="fdx"
                     /locus_tag="PK856_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HRL02115.1"
                     /translation="MPKITVLPHPVLCPEGAEITDAPVGQTICDVLLEHHVDIDHACE
                     KSCACTTCHVVIKQGFNSLEDATELEEDLLDQAWGLTPTSRLSCQARVADVDLVVELP
                     KYTINHAREH"
     gene            1455..1649
                     /gene="iscX"
                     /locus_tag="PK856_06810"
     CDS             1455..1649
                     /gene="iscX"
                     /locus_tag="PK856_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HRL02116.1"
                     /translation="MKWTDSLRIAEDLADTHSDLDPTTIRFTQLRQLVLDLPDFDDVP
                     ERCGEKILEAIQQAWIDEVA"
     gene            complement(1680..2450)
                     /locus_tag="PK856_06815"
     CDS             complement(1680..2450)
                     /locus_tag="PK856_06815"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HRL02117.1"
                     /translation="MDKPALRFSIAPDTHPDSLPGWHIFLRHVQKQLDVSIKIADYAN
                     FSDLRQGLEQDQVDMIFASAADTSYLVNHKDFLALAQPESNVTEIVVLCRADAPYTQI
                     EDIVGNVRTIVFTDSPEIKHLGLILLEPADLNHNEVSFMKADNHLLTAKKLVNGEADL
                     AFIPTEIYMGWSETLRKSVRPLVQTLKDDVEGLNHVLLLSPSQAQLAHPLKQILSDIN
                     QNSQHASLLSDINISQWQVLQDNSDIDFLIDLVDTLQI"
     gene            complement(2453..2962)
                     /locus_tag="PK856_06820"
     CDS             complement(2453..2962)
                     /locus_tag="PK856_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009427603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRL02118.1"
                     /translation="MMAYLWKDMDKADLANQSAYQEMDHQFQDGQKRKILLVDEEVPY
                     PDGRMIVSRTNLQGDITHANKAFVDISAYEREELIGAPQCILRHPDVPSEIFADMWAT
                     IQAGKSWFGCVKNLRKDGRYYWVYASVNPNYRQGKIVAYTSVRRKPSRAKVAETMALI
                     QQINQQKAS"
     gene            complement(3188..>3933)
                     /locus_tag="PK856_06825"
     CDS             complement(3188..>3933)
                     /locus_tag="PK856_06825"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HRL02119.1"
                     /translation="TYNGAADANEQPYVLVGKGITFDTGGISLKPGAGMDEMKYDMFG
                     AASVIASFCAAAEMKLPINLKAIVPTCENMPSGGAAKPGDIVKAMNGISIEILNTDAE
                     GRLILCDALAFAEQFKPQAVVDVATLTGACIIALGHEVSGVMGNNQDLVNELLAVGTE
                     VNDRVWQLPLFDDYRDLLKSNFADLANIGGRPAGTITAAAFLSYFTENYAWAHLDVAG
                     TAWASGASKGATGRPVPLLVNYLVKQAEK"
BASE COUNT         1044 a         1043 c          909 g          937 t
ORIGIN      
        1 atgtgcaaca agcggtggca accttctttg gccaaacccc gctcaacaac ctgaacccag
       61 acgaagtagt cgctttgggc gcggcgatac aagcgaatgt gttggcaggc aacaaagccg
      121 atgatgaatg gttgttgctt gatgtgaccc ccttgtcttt gggcttggaa acctatggcg
      181 gcttggtcga aaaagtgatt cctcgcaatt cgaccatccc aacggcgatg gcacaagaat
      241 tcaccacgtt taaagacggt caaaccgcga tggcgattca tgtggtgcaa ggcgagcgcg
      301 aattggtaag cgattgtcgc tccctcgccc gctttaccct gcgtggcatt ccgcccatgg
      361 tggctggtgc agcacgcatc cgcgtgacct tccaagtcga tgccgatggc ttgttgtcgg
      421 tatcggcaca agaacaaacc acaggcgtac aggccgctat tgaagtgaaa ccttcatacg
      481 gcttagacga caccaccatc acccaaatgc tcaaagattc gatgcagttt gcacaagaag
      541 acatggcaca gcgggcacgc caagaagcct tggtagaagc ggaaagcatc gttactgcgg
      601 tttcaactgc attgatgagc gatggtgatt tgctgactga gtctgagcgc agcgacatcg
      661 aggtggtcat caaacgctta caactgcaaa tggctcaagg cgatgctgca agcatccgtg
      721 ccagtgtgga agccttgaac catgccaccg atgcttttgc agccgcacgc atggaccgca
      781 acattcagcg cgccttaaca ggccaaaaca tcgccgatat ttaaccagaa agcggcctgt
      841 aatgtctttt agccctgagc aaatcagcga attactgcaa ctcccctttg tgggcaaaac
      901 cgtcatccaa cgcttacaag aagtgggctt ggatgatgtg cacgccttgg ccaacagcaa
      961 tgcaacagag gtattggaag tgattgccgc ccatttgcac agcagctgct ggaaaaacag
     1021 cccacaagcc aagcaatcca tccacaacat cattgcttgg gcaaaacaat acccataagg
     1081 atttatatgc caaaaattac tgtgttgcct catccagtgc tgtgcccaga aggcgcggag
     1141 attaccgatg cacctgttgg tcaaaccata tgtgacgtgt tattggaaca ccatgtggac
     1201 atcgaccatg catgtgaaaa atcttgcgct tgcaccactt gtcatgtggt gattaaacaa
     1261 ggctttaaca gcttggaaga tgcgaccgaa ttggaagagg atttgcttga ccaggcttgg
     1321 ggcctgacgc ctacttcgcg cctgagttgc caagcccgtg tggccgatgt ggatttggtt
     1381 gtagaattac cgaaatacac catcaaccac gcccgcgaac actgataagc ggcctgtaaa
     1441 aaaggagcaa ccccatgaaa tggaccgata gcctgcgcat cgctgaagac ttggccgata
     1501 cccactcaga cctagacccc accaccatac gctttaccca attgcgccaa ttggtgttgg
     1561 atttgcctga ctttgatgat gtcccagaac gctgtggtga aaaaatcctc gaagccatac
     1621 aacaggcttg gatagacgaa gtcgcttaat aaaaaaacct gccactggca ggttttttat
     1681 tagatttgca aggtatccac caaatcaatc aaaaagtcta tgtcgctgtt atcttgcaaa
     1741 acctgccact ggctgatgtt gatgtcactg agcaggctgg catgttggct gttttgattg
     1801 atgtcgctca aaatctgttt aagaggatgt gccaactgcg cctgcgatgg cgacaacaac
     1861 aacacatgat ttaaaccttc cacatcgtct ttcagggttt gcaccaaagg ccgcaccgat
     1921 ttgcgcaaag tctcactcca acccatgtaa atttcagtcg gaatgaatgc caaatccgcc
     1981 tcaccattca ccaatttctt ggccgtcaac aaatggttat cggctttcat gaagctgact
     2041 tcattgtggt tcaaatcggc aggctctagc aaaatcaaac ccaaatgctt gatttcagga
     2101 ctatcagtaa acacgatggt gcgcacattt cccacgatgt cttcgatttg cgtgtaaggt
     2161 gcatcggcac gacacaacac aacaatctca gtgacattgc tttcaggctg tgccaaagcc
     2221 aaaaaatcct tgtggttcac caaataactg gtatcggccg cgctggcaaa aatcatgtct
     2281 acctgatctt gctccaagcc ttggcgcaaa tcactgaaat tggcgtaatc ggcaatcttg
     2341 atgctgacat ccaactgttt ttgcacatgg cgcaagaaaa tgtgccaacc tggcaagctg
     2401 tctggatggg tatcgggagc gattgagaaa cgcaatgcgg gtttgtccat gtttaagatg
     2461 ccttttgttg gttgatttgc tgaatcagtg ccatggtttc tgccactttc gcccgtgaag
     2521 gcttgcgtct gaccgaagta taggccacga tttttccttg acggtagtta gggttcaccg
     2581 aggcatacac ccagtaatac cgaccatctt tacgcagatt tttcacgcaa ccaaaccagc
     2641 tcttaccggc ctgtatggtt gcccacatat cggcaaaaat ctcactgggc acatcgggat
     2701 gacgcaaaat gcattgtggg gccccaatca actcctcacg ctcgtaagcc gaaatgtcga
     2761 caaatgcctt gttggcatgg gtgatgtcac cttgcaaatt ggtgcgcgac acaatcatgc
     2821 gcccatcagg ataaggcact tcttcatcca ccaataaaat cttacgcttc tgaccatctt
     2881 gaaactggtg gtccatttct tggtaagcgc tttgattggc caaatcagct ttgtccatgt
     2941 ctttccaaag atacgccatc atgactcccc tctgtcatca aaccctgttt gctcccaagc
     3001 caatttgact tgagaaagag gaaacggcag ggttcaactt cgatgcttta aaaatgtaaa
     3061 aatgttgtgt gcagcaagaa gtgggtcaat aaaaaaagcc ctgctgcaaa gcagcgggct
     3121 ttcgtatggt attgagcatt cacttgattc aaatcaaatg actactggat aaaaaccatg
     3181 ctacatctta tttttcagct tgtttcacca aataattcac caacaaaggc acagggcggc
     3241 ctgtagcccc tttgctcgcg ccactggccc atgccgtgcc tgccacatcc aaatgcgccc
     3301 aagcatagtt ttcagtgaag taagacaaga acgcagccgc ggtaatcgtg cccgcaggac
     3361 ggccaccaat attggccaaa tcggcaaaat tgcttttgag caaatcgcgg tagtcatcaa
     3421 acaaaggcaa ttgccacacg cggtcgttca cttccgtacc taccgccaac aattcattca
     3481 ccaagtcttg attgttgccc atcacaccgc tcacttcatg gcccaaagca atgatgcacg
     3541 cgccggttaa agtggccaca tccaccacgg cttgtggttt gaactgctcg gcaaacgcca
     3601 atgcatcgca caaaatcaaa cggccttcgg catcggtatt caagatttca atgctgatgc
     3661 cattcatcgc tttcacaatg tcgcccggct tggccgcgcc accagaaggc atgttttcac
     3721 acgttggcac gatggctttc aaattgatgg gcaacttcat ttctgcggca gcacagaaag
     3781 acgcgatcac cgaagccgca ccaaacatgt cgtatttcat ctcgtccatg cctgcacccg
     3841 gcttcaaaga gatgccgccc gtgtcaaacg taatgccttt gcccaccaac acataaggct
     3901 gctcattggc atcggccgcg ccgttgtaag tca
//
LOCUS       DAOMVK010000183         3925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_6693_length_3925_cov_3.630749, whole genome shotgun sequence.
ACCESSION   DAOMVK010000183 DAOMVK010000000
VERSION     DAOMVK010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6693_length_3925_cov_3.630749"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1929
                     /locus_tag="PK856_06830"
     CDS             <1..1929
                     /locus_tag="PK856_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009116648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRL02120.1"
                     /translation="ERESHAVYFLNQQSITRLDVMRFMAHGIEPEPSSPTQGNGDGKE
                     ASMDNALQQYTTNLNTEVLAGRIDPLIGRAQEVLRMTQILGRRRKNNPLLVGEAGVGK
                     TALAEGLAYQIVHRQVPENLSNAIVFSLDMGALLAGTKYRGDFEARIKAVLKELTELP
                     NAILFVDEIHTIIGAGSVSGGTMDASNLLKPALAKGQLRCIGATTYQEYRTIFDKDHA
                     LSRRFQKVEVGEPTAAETVAILRGLKSSFENFHQVKYTQEALEAAVELSVRYINERFL
                     PDKAIDVIDEAGAAQKVKPKSKQRKTIAREQIEEIVAKMARIPETTVSTDDKNVLKNL
                     NRTLKSMVFGQEKAIEALVNAIKMSRSGLGQPEKPIGSFLFSGPTGVGKTEVSKQLAF
                     ALGVPFIRFDMSEYMERHAVSRLIGAPPGYVGYEEGGLLTEAISKQPYSVLLLDEIEK
                     AHPDIFNVLLQVMDHGTLTDNNGRKADFRNVVIIMTTNAGAESLAKPGIGFASSKAVG
                     DEMGAINKLFSPEFRNRLDAIVSFASLDENIIQQVVDKFLLQLEQQLLAKNVEIEFTD
                     GLKAYLAEKGFDPLMGARPMSRLIQTTIRKALADELLFGKLAEGGRVTVDWDSKTNDV
                     QLIFPTTKSDRELEQAVH"
     gene            complement(1964..2293)
                     /locus_tag="PK856_06835"
     CDS             complement(1964..2293)
                     /locus_tag="PK856_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HRL02121.1"
                     /translation="MNFNDYLASFPTVDHLSGLNVLDESGAVIHHIPAVAGKLGSLKL
                     YNALAERFDGQLHAQAAEQGLLWFAEHVTDAKAHSGKHPNVDLLLLVQAKQKQYRLQP
                     VATYSGL"
     gene            complement(2312..2950)
                     /gene="upp"
                     /locus_tag="PK856_06840"
     CDS             complement(2312..2950)
                     /gene="upp"
                     /locus_tag="PK856_06840"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HRL02122.1"
                     /translation="MSNLHIIDHPLVQHKLSLMRQQECSTYKFRTLTNELARLMAYEA
                     TRDFPVEDFDMVGWEGNTIQGKQIKGKTVTIVPILRAGIGMLDGVMDMLPTAKISVVG
                     LQRDEETLQPVPYFDKFVPKVEERPALIIDPMLATGGSMVATIDLLKSKGCKDIKALV
                     IVAAPEGVKVVNDAHPDVKIYTAALDSHLNENGYIIPGLGDAGDKIFGTAHH"
     gene            complement(2967..3611)
                     /locus_tag="PK856_06845"
     CDS             complement(2967..3611)
                     /locus_tag="PK856_06845"
                     /inference="COORDINATES: protein motif:HMM:NF021141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2459 domain-containing protein"
                     /protein_id="HRL02123.1"
                     /translation="MMRRLWRAVWRLTLALLSVIMMYGLAAWILGSLSTQASSQFVHD
                     TEPCHDVYVVSNGVHLNLFLPMRSQAMDWQRFLPASLGDTDAPMVQIGWGSRAFYTQV
                     PTWADLTPQIAAQALFYDDAVLYVRPASIPVADAKHVRRVRMCQQELQNVSLDIAKQF
                     ANTTPVSGFHDFYPAQGHYTPFMTCNEWMRLRLQHRSMPIWSPFDQAILNHLPL"
     gene            complement(3614..3922)
                     /gene="grxD"
                     /locus_tag="PK856_06850"
     CDS             complement(3614..3922)
                     /gene="grxD"
                     /locus_tag="PK856_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HRL02124.1"
                     /translation="MSVQQTIQDIVNSHKVVLFMKGDKRFPQCGFSARAVQILQASGC
                     EEFHTVDVLMDEGIRQGIKEYANWPTIPQLYVNGEFLGGSDIMLEMFEAGELQSALAE
                     "
BASE COUNT         1009 a          872 c         1024 g         1020 t
ORIGIN      
        1 gaacgtgaat cacatgccgt ttatttttta aatcagcagt caatcacgcg tttggatgtg
       61 atgcgcttta tggctcatgg tattgagccc gagccttcat cacccacaca agggaacggg
      121 gatggaaagg aagcttccat ggacaatgcc ttacagcaat acaccaccaa tctgaacact
      181 gaagttttgg caggccgcat cgatccactg atagggcggg ctcaagaagt attgcgcatg
      241 acccaaattt tggggcgacg ccgtaaaaac aatcccttgc ttgtgggcga ggctggcgtg
      301 ggtaaaactg ccttggctga aggcttggcc taccaaattg tacaccgaca agtgcctgaa
      361 aacctcagca atgccatcgt gttctccttg gatatgggtg ctttactggc tggtaccaaa
      421 taccgaggcg attttgaggc gcgcatcaaa gccgtgttga aagaattaac cgagttgcct
      481 aatgccatct tgtttgtgga tgaaatccat accattattg gagcgggcag tgtgtctggt
      541 ggcaccatgg atgcttccaa cttattgaag cctgctttgg caaaaggaca gttgcgttgc
      601 attggagcga ccacttacca agaataccgc accatttttg acaaagacca tgctttgagt
      661 cgtcgctttc agaaggtgga ggttggtgaa ccaacagcgg cggaaactgt ggccattttg
      721 cgtggtttga agtcttcctt tgagaacttc catcaggtga aatacacgca agaggcttta
      781 gaagcggctg ttgagctgtc ggtgcgttac attaatgagc gttttttgcc cgataaggca
      841 attgatgtga ttgatgaagc gggtgctgcg caaaaagtaa aacccaaatc aaaacagcgc
      901 aaaaccattg cacgtgaaca aatcgaagag attgtggcga aaatggcacg tatccctgaa
      961 accaccgtgt ctaccgatga caaaaacgtg ttgaaaaatt tgaaccgtac cttaaagtct
     1021 atggtgtttg gtcaggaaaa agccattgag gccttggtca acgcgattaa aatgtcgcgt
     1081 tcgggtttgg ggcagcctga aaaaccaatc ggtagcttct tattctcagg ccctacaggg
     1141 gtgggcaaga ctgaagtgtc caagcaactg gcctttgccc taggtgtgcc attcattcgt
     1201 tttgacatgt cagagtatat ggagcgacat gcggtatcac gcttgatagg ggcgcctcca
     1261 ggatatgtgg gctatgaaga aggtggcttg ttgactgagg ccatcagcaa gcagccatat
     1321 tcggtgttgt tgttagacga gattgaaaaa gcccatcctg atattttcaa tgtgttattg
     1381 caagtgatgg atcatggcac tttaaccgat aacaatggcc gtaaagccga ttttcgcaac
     1441 gtggtcatca tcatgaccac gaatgcagga gcggaaagtt tggccaagcc tggcatcggt
     1501 tttgccagct ccaaagcagt gggtgatgag atgggggcga ttaacaaatt gttcagcccc
     1561 gagttccgta accgtttgga tgcgatagtg tcgtttgctt cattggatga aaacatcatt
     1621 caacaagtgg tggataaatt cttgttgcag ttggaacaac aattactggc caaaaatgtg
     1681 gaaattgaat tcaccgatgg cttaaaagct tatctagctg agaaaggctt tgatccgttg
     1741 atgggggccc gtccaatgag ccgtttgata caaaccacca tacgcaaagc gctggctgat
     1801 gaattgctgt ttggaaagtt ggctgaaggt ggtcgtgtga ccgtggattg ggacagcaaa
     1861 accaatgatg tgcagttgat ttttccaacg accaaaagcg acagagaatt ggaacaagct
     1921 gtgcattaat acgataaaaa ccgccgaaag gcggtttttt tatttacagg ccgctatatg
     1981 ttgcgacggg ttgtaggcgg tattgttttt gtttggcttg taccaataat aataagtcta
     2041 cattggggtg tttgccagag tgggctttcg catcggtcac atgttcagcg aaccacagca
     2101 agccttgctc ggctgcttga gcatgaagct gaccatcaaa tcgttctgct aaggcattgt
     2161 agagctttaa actgcccaac ttacctgcaa cggcagggat gtgatggata accgcccctg
     2221 attcatccaa gacgttcagg ccgcttaaat ggtcgacagt tgggaaactt gccaagtagt
     2281 cgttgaaatt catgctgtat ccttagggta gtcagtggtg tgcagtgcca aaaatcttgt
     2341 caccggcatc gcccaaacca ggaatgatgt agccattttc attcaagtgg ctgtccaatg
     2401 ctgcggtgta aattttcaca tcaggatggg catcgttcac cactttcacg ccttctggtg
     2461 cggccacaat caccaaagct ttgatgtctt tacagccctt gcttttgagc aaatcgatgg
     2521 tggcaaccat agaaccgcct gttgccaaca ttgggtcgat gattaaggca gggcgctctt
     2581 cgactttggg cacgaatttg tcgaaataag gcacgggttg cagggtttct tcatcgcgtt
     2641 gcaaaccgac cacgctgatt ttggctgttg gcaacatgtc catgacgcca tcgagcatgc
     2701 cgatgcctgc gcgcaagatc ggcacgatgg tcacggtttt gcctttgatt tgcttgcctt
     2761 gtatggtatt gccttcccag cccaccatat caaaatcttc tactggaaag tcacgtgtgg
     2821 cttcataagc catcaggcgg gccaattcgt tggttaaggt gcggaatttg taggttgagc
     2881 attcttgctg gcgcatcagg ctgagtttgt gttgtaccaa agggtggtca atgatgtgta
     2941 ggttagacat ggtgtggcct taaactttaa aggggtaaat ggttgagaat ggcttggtcg
     3001 aatggagacc aaataggcat gctgcggtgt tgtaggcgca gccgcatcca ttcattgcag
     3061 gtcataaaag gtgtgtaatg accttgagca gggtaaaaat cgtgaaatcc agatacgggt
     3121 gtggtgttgg caaactgttt ggcaatgtct aggctaacat tttgcaattc ttgctgacac
     3181 attcgcacac ggcgcacatg cttggcatcg gcgacaggga tgcttgcagg gcgaacatac
     3241 aggacggcat catcataaaa caaggcttgt gcggctattt gaggagtcaa atctgcccaa
     3301 gtcggaacct gagtataaaa tgcgcgactg ccccagccta tttgtaccat gggtgcatcg
     3361 gtatcgccca agcttgcggg cagaaagcgc tgccaatcca tggcttgcga gcgcatgggc
     3421 agaaacagat tcaaatgcac accattgctg accacataca cgtcatgaca cggttcggtg
     3481 tcatgaacaa attggctgct ggcttgggtc gatagacttc ccaaaatcca agcagccaag
     3541 ccgtacatca tgatgactga taacagggcg agtgtgagtc gccaaaccgc gcgccacaat
     3601 cgcctcatca tgcttactct gctaatgcgc tttgcaattc gccagcttca aacatttcca
     3661 acatgatgtc tgaaccgcct aagaattcac cattcacata caattgtgga atggtaggcc
     3721 agttggcgta ttctttgatg ccttggcgta tgccttcgtc catcaacaca tccactgtgt
     3781 gaaattcttc acagccagaa gcctgtaaaa tttgcaccgc acgggcagag aagccacatt
     3841 gtgggaaacg tttgtcgcct ttcataaaca acaccacttt gtggctgtta acgatgtctt
     3901 gtatggtttg ttgcacgctc atgct
//
LOCUS       DAOMVK010000184         3850 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_6877_length_3850_cov_2.881686, whole genome shotgun sequence.
ACCESSION   DAOMVK010000184 DAOMVK010000000
VERSION     DAOMVK010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6877_length_3850_cov_2.881686"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3083
                     /locus_tag="PK856_06855"
     CDS             <1..3083
                     /locus_tag="PK856_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HRL02125.1"
                     /translation="LDILPLFARLSAQEQHRIFHPTGQARRVILATNIAETSLTVPGI
                     RFVIDTGLARVKRYSARAKVEQLHIEKISQAAARQRAGRCGRVAAGVAIRLYAEDDFN
                     TRSEYTDPEILRSNLAAVILRMVSLQLGDVAAFPFLQAPEQRYINDGFQVLLELGAVD
                     DNNRLTKLGEQMAKLPVDPKIARMLLAAKRLNCVPEILIIVSALSIQDPRERPFEARD
                     AADKMHERFKDKESDFLSYLYLWEFFLQTRAQKSYKQTMQVLQQHFLSGPRMREWREL
                     HQQLEQTARELELLLNTGAANESVSSVETNKPNAKFQKSEHAVDDKTVNKMRQHGGTE
                     TSYDQIHRALLTGLIAQVGLKASDSHEYQGARGSRFHLFPASNLFKAKPKWVMAAELM
                     ETTKLYARDVAKINPEWIELEAPHLVKKHYFEPHWSKERGEVLASERVTLYGLTVMPR
                     RLVSYGRIAPEEARELFIRGALVEQQAHFQDAFFKHNAKLIKDITELEHKSRKQDVLV
                     DDEVLFDFYQQRIDVSYAQGKKTYPIVDAATFHAWLKLQADDKALYLSRDDLMQHSAT
                     HITDAQFPKTFKTADGAFKLSYRFEPHHPLDGVTMTVPLPVLNRLDDKQLEWLVAGLI
                     REKLQLLIKALPKQVRRVCVPVPDFVTRFLEREPNQHEPILPQLAAFIAREFGDVRIL
                     EQIDFDAWAAAELPAHCFFNFRVIDDGGQELAMGRNLLALQQQLGHAAAVTFSDNANE
                     FEQENVTTWSIGKLPDSIKFARGKQQLTGYLGLQIQSDDSIALKLFDTQGAADEAHYH
                     GVIALMAKELKDQIKDLNKGLASFTQVAMLLKHLGVDALRDDITRAVCQRAFIGDDDL
                     PRNDKDFKEQIKRARSRLPAVKEALNKYLLEVAQEYAPLVLRLQKHPLKQVLQQQLQD
                     LIHAGFASETPWSQWPHLARYLKGMNLRMDKFSGNPARDAQREADIQVLHQQWQQRIS
                     DLQRSHQDIPSDLKLFRWGIEELRVSLFAQELKTPYPVSVKRLQKQWTELTRVV"
     gene            3215..3754
                     /locus_tag="PK856_06860"
     CDS             3215..3754
                     /locus_tag="PK856_06860"
                     /inference="COORDINATES: protein motif:HMM:NF016874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HRL02126.1"
                     /translation="MTSLDIYWATPAVFWPDSEAWVAAQKRELQDFARAQNTDLHVLS
                     PIDTALEQKCCVDESIFLSNVAMVQQATCVIADVSPFRSMEPDVGTALQIGLATQMGK
                     PVYLYSKHADVSLQAAYQAQMNGEGYVMFEGKPTQVEDFGHPVNLMLMHDLAGNKRTV
                     FASLQAALQQVLSNMVKPV"
BASE COUNT          973 a         1027 c         1023 g          827 t
ORIGIN      
        1 atctggacat tttgcctttg ttcgcgcgct tgtcggcgca agagcaacac cgcatattcc
       61 accccacagg ccaagcgcgg cgggtgatat tggccaccaa catcgccgaa acctcgctca
      121 ccgtgccggg tatacgtttt gtcatcgaca caggtttggc gcgggtgaaa cgctattctg
      181 cgcgcgccaa agtggaacaa ctgcacatcg agaaaatctc ccaagccgcc gcgcgccaac
      241 gcgcaggtcg ttgtggccgt gtggccgcgg gcgtggccat ccgcttgtat gctgaagacg
      301 atttcaatac ccgttcagaa tacaccgacc ccgaaatcct gcgctcgaat ttggccgcgg
      361 tgattttgcg catggtcagc ctacaattag gcgatgtggc cgcgtttcca ttcctacaag
      421 cgcctgagca acgctacatc aacgatggtt ttcaggtgtt gctggaattg ggcgcggtag
      481 acgacaacaa ccgcctcacc aagctcggtg aacaaatggc caagctgcct gtcgacccga
      541 aaatcgcgcg catgttgctc gcggccaaac gcctgaactg tgtgcctgaa atcctgatta
      601 tcgtgtcggc cttgtcgata caagacccac gcgaacgccc atttgaagcg cgcgatgctg
      661 ccgacaaaat gcacgagcgt ttcaaagaca aagaatccga tttcttatcg tatttgtatt
      721 tgtgggaatt tttcttgcaa acgcgcgcgc aaaaatccta taagcaaacc atgcaagtgt
      781 tgcaacaaca cttcttgagc gggccgcgca tgcgcgaatg gcgcgaattg caccaacaat
      841 tggagcaaac cgcgcgtgaa ttggaattgt tgctcaatac aggtgcggcc aacgaaagcg
      901 tcagcagcgt cgaaaccaac aagccgaatg ccaaattcca aaagagcgaa cacgcggtag
      961 acgacaaaac cgtgaacaaa atgcgccaac acggtggcac tgaaaccagt tacgaccaaa
     1021 tacaccgcgc cttgctcaca ggcttgattg cccaagtcgg cctgaaagcg tctgacagcc
     1081 acgaatacca aggcgcgcgt ggcagccgct ttcatttgtt ccccgcctcc aatttgttca
     1141 aagccaagcc caaatgggtg atggcggcgg aattgatgga aaccaccaag ctgtatgcgc
     1201 gcgacgtggc gaaaatcaat cccgaatgga tagaattgga agcgccgcat ctggtgaaaa
     1261 aacactattt tgaaccgcat tggtccaaag agcgcggcga agtattggct tcggagcgcg
     1321 tgactttgta cggcttgacc gtgatgccac gccgcttggt cagctatggc cgcatcgcgc
     1381 ctgaggaagc gcgtgaattg ttcatacgcg gtgcgctggt tgagcagcaa gcgcattttc
     1441 aagacgcatt tttcaaacac aatgcaaagc tcatcaaaga catcaccgag ctggaacaca
     1501 aatcgcgcaa gcaagacgtg ttggtagacg atgaggtgtt gttcgatttt taccaacagc
     1561 gcatagatgt gagctatgcc caaggcaaaa agacctatcc gatagtggat gcggccacct
     1621 tccatgcttg gctcaagtta caggccgatg acaaagcgct gtatttgagt cgcgatgatt
     1681 tgatgcaaca ctcggccacc cacatcaccg atgcgcaatt tccgaaaacc ttcaaaaccg
     1741 ccgatggtgc gttcaagctt tcctatcgct ttgagccaca ccacccactc gacggcgtca
     1801 ccatgaccgt gcccttgcct gtgctcaatc gcttggacga caagcaattg gaatggctgg
     1861 tggcgggctt gatacgcgaa aaattgcaat tgctgattaa ggctttgccc aagcaagtgc
     1921 gccgtgtgtg tgtgcctgtg cccgattttg tcacgcgctt tttggagcgc gagccgaatc
     1981 aacacgagcc tatcttgccg caattggcgg cgtttatcgc gcgcgagttt ggcgatgtgc
     2041 gcatcttgga gcaaattgat tttgacgcgt gggccgcagc cgaattgcct gcccattgct
     2101 tcttcaattt ccgcgtcatc gacgatggtg gacaagaatt ggcgatgggc cgcaacttgc
     2161 ttgccttgca acagcaattg ggccatgccg ccgccgtgac tttcagcgac aatgccaacg
     2221 aatttgagca agaaaacgtc accacttgga gcataggcaa gctgcccgat tcgattaaat
     2281 ttgcccgcgg caaacagcag ttaactggct atttgggttt gcaaatccaa agcgatgaca
     2341 gcattgcctt gaaactgttc gacacccaag gcgcggcgga tgaagcgcat taccacggcg
     2401 tgattgcgct gatggccaaa gagctcaaag accaaatcaa agacttgaac aaaggcctcg
     2461 cttccttcac ccaagtggcc atgttgctca aacacttggg cgtggacgcg ctgcgcgacg
     2521 acatcacccg cgccgtgtgc caacgcgcct tcatcggcga cgacgatttg ccgcgcaacg
     2581 acaaagattt caaagagcaa atcaagcgcg cgcgcagccg tttgcctgcg gtgaaagaag
     2641 ccttaaacaa atacctgttg gaagtggcgc aagaatacgc gcccttggtg ttgcgtttgc
     2701 aaaaacaccc gctcaaacag gtgttgcaac agcaattgca agacttgata cacgcaggct
     2761 ttgccagcga aacgccgtgg agccaatggc cgcatttggc acgttatttg aaaggcatga
     2821 atctgcgcat ggacaaattc agcggcaacc ctgcacgcga tgcccagcgc gaagccgaca
     2881 ttcaagtgtt acatcaacaa tggcagcagc gcatcagtga tttgcaacgc agccaccaag
     2941 acattccaag cgatttgaaa ctgttccgct ggggcataga agaattgcgc gtgtctttgt
     3001 ttgcgcaaga attgaaaacg ccgtatcccg tgtcggtgaa gcgtttgcaa aagcaatgga
     3061 cggaattgac gcgcgtggtg taaagctgag cggcctgtaa accgcagcat ggtttacagg
     3121 ccgcttgttg agtatgaaat cctgctacaa cacatttaca agcggcattt aggcgatgtt
     3181 tgctgcaata gccgcccata acaaggggaa acacatgaca tctttggaca tctactgggc
     3241 cacgcctgcg gtgttttggc ccgattcaga agcatgggta gcggcgcaaa agcgcgagct
     3301 gcaagacttt gcccgtgcgc aaaacaccga tttgcatgtg ttgtcgccga tagacacggc
     3361 tttggaacaa aaatgctgcg tggacgaatc gattttcttg agcaatgtgg cgatggtgca
     3421 gcaagccacg tgcgtgattg ccgatgtgtc accgtttcgg agcatggagc cagacgtggg
     3481 cacagcctta caaatcggct tggccacgca aatgggcaag cctgtgtatc tgtattccaa
     3541 acacgccgac gtgagcttac aggccgctta tcaagcgcaa atgaatggcg agggttatgt
     3601 gatgtttgag ggcaagccca cccaagtcga agactttggc catcctgtga atttgatgtt
     3661 gatgcacgat ttggcaggca acaaacgcac cgtgtttgcc agcttacagg ccgcattgca
     3721 acaggtattg agcaatatgg tgaaacctgt ctagccttgc tgtgtgttgg ttgacaccat
     3781 gttcttacag actgagcata cccacattgc taaaaacagg ccgcatgact tggacaccca
     3841 agcatgtggc
//
LOCUS       DAOMVK010000185         3846 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_6887_length_3846_cov_3.593511, whole genome shotgun sequence.
ACCESSION   DAOMVK010000185 DAOMVK010000000
VERSION     DAOMVK010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6887_length_3846_cov_3.593511"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /gene="ispE"
                     /locus_tag="PK856_06865"
     CDS             complement(<1..624)
                     /gene="ispE"
                     /locus_tag="PK856_06865"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRL02127.1"
                     /translation="MTLQNVPADAQAFLAPAKLNLMLHITGRRDDGYHLLETVFTFIG
                     LYDTVYLRRRDDGDIVLHTLIEGVDAEQDLSVRAARILQQASGSKFGADIWVEKRIPM
                     GGGLGGGSSDAATVLLALNHLWQTQLSREALMALGLQLGADVPVFVFGRNSFASGIGE
                     ELSELSIPNQWYVVVKPDVHVSTPKIFAHGRLTRDSAPSIIRVFPRVQ"
     gene            complement(643..1230)
                     /locus_tag="PK856_06870"
     CDS             complement(643..1230)
                     /locus_tag="PK856_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HRL02128.1"
                     /translation="MKRWLRGSATVLVLTVLAACQTAPIKPAVWQAGSRDALNFVASG
                     RLAVKQNEKGSYANFDWDSNGRMQQLSVSTPLGNTVGELCQDGTGVVATASNGEVYQA
                     VTASELSERLMGFAIPVESLDLWAHGYYAENTPHTVDEQGRLLQAGWKIERQTDGEGH
                     PRLLVLDNQQLSIRLAFNDYQTGASEADTTFCQRK"
     gene            complement(1227..3134)
                     /locus_tag="PK856_06875"
     CDS             complement(1227..3134)
                     /locus_tag="PK856_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02129.1"
                     /translation="MASWHQRLLPLAACIAVIATTPALAQAEIKKPADQSVSPLTKDI
                     NTSLDKLQKPTAQKARDAADEKIRLQKITQDANAFAGMLASELSLLQGNTGGALGAYV
                     VMFERTGRPELAERAMQIAINGGGYNEAQLVLERWRKVEPVPSQLQKQMAWELDASRG
                     NVGTALNDFPAVLTQANEAQARRVFLLLSQLSLAGPQAAQQGYDNVMAATKKYPNMSE
                     AAVVEAMYAALSRHDKQAVNALNRLASLDADIRPTTQLAIGLLAQKSPQVINQFFSQA
                     DASKLSDMWRELQIDTLVRTEQFDKAFQLIQNELSKNPTPELQLQAGILAMKKKDYRD
                     ANVFLSKVYQGGDVAQRNRAATLLAVVAFGQDQTEKGAEWAAKIDDAEFQFDKSVILA
                     TAAAERKDWPEMRKQLDKLQASKPAANSLYDQGQAQRLELLWIYNQQDPMVVYREYSK
                     KIDEQLASGAPDVNLLAELYSQRGILLTDKLKRPEDGLKDLQKQLILRPNDANAMNVL
                     GYTLLSQEATREQGFALVEQAYKLEPQAPHINDSLGWAYYLKGQPEKALAYLKFAYDK
                     MPDAEVAAHLVDTYMALGQERKARDFAKKGLELDSTHAVLLETLARHHLQPKQAPSAV
                     AASQADTGVQP"
     gene            3278..>3846
                     /gene="mutM"
                     /locus_tag="PK856_06880"
     CDS             3278..>3846
                     /gene="mutM"
                     /locus_tag="PK856_06880"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HRL02130.1"
                     /translation="MPELPEVETTRNGIAPFTEGQTIDHVVVRQSKLRWPIPEDLNQH
                     IAACKIVSVSRRAKYLLIHLPHGVIMVHLGMSGSLRVFEADLAPEAGKHDHVDLVLQN
                     GTVLRYHDPRRFGAWLWFSGAVETHPLICKLGPEPLEEAFSAEYLQAALSTQKRALKL
                     ALMDNHVVVGVGNIYANEVLFCCKLLPTT"
BASE COUNT          913 a         1079 c          882 g          972 t
ORIGIN      
        1 ctgcaccctt ggaaagaccc gtattatgct aggggcagaa tcacgtgtca agcgcccgtg
       61 cgcaaaaatt ttcggtgtag acacatgcac atcgggcttc accaccacat accattgatt
      121 tggaatggaa agctcactga gctcttcacc gatacccgag gcaaaagaat tgcgcccaaa
      181 aacaaacaca ggcacatcag ctcccagttg caaacccaaa gccatcagcg cttcacgcga
      241 gagttgggtt tgccacaaat gattcaaggc cagcaataca gttgctgcat cagatgaacc
      301 accgcccaag ccgccgccca tgggtatccg tttctctacc caaatgtccg ccccaaactt
      361 gctgccgctg gcttgttgca atatccgtgc cgcgcgcaca ctcaaatctt gctcggcatc
      421 taccccttca atcaaggtgt gcaacacaat gtcaccatcg tcccgacggc gcaggtagac
      481 ggtatcgtac aggccgataa aggtgaacac ggtttccaat aagtggtaac catcgtcacg
      541 gcggcctgta atgtgcaaca tcagatttaa tttggcaggt gccaaaaaag cctgcgcatc
      601 ggcaggcacg ttttgaagcg tcatgaggct tctttccttc atttatttac gctgacaaaa
      661 agtagtgtct gcctctgatg cacccgtttg gtaatcatta aaagccaaac gtatgctcaa
      721 ttgctggtta tccaacacca acaaacgcgg atgaccttca ccgtctgttt ggcgttctat
      781 cttccaaccg gcctgtaaca aacgtccttg ctcatccaca gtgtgcgggg tgttttcagc
      841 ataatagcca tgtgcccaca aatccaacga ctctacagga atggcaaaac ccatcaaacg
      901 ctccgacaac tcacttgccg tgaccgcttg gtaaacctca ccattgctgg cggtggccac
      961 cacaccagtg ccgtcttggc acaactcgcc taccgtattg cccaaaggtg tgctcacact
     1021 caattgctgc attctgccat tgctgtccca atcgaaattg gcatacgatc ctttttcatt
     1081 ttgtttcact gccaagcgcc ctgatgcgac aaaattcaag gcatcacgag aacccgcctg
     1141 ccacacggcg ggcttgattg gtgcagtctg acacgccgcc aagactgtca acaccaaaac
     1201 cgtggcactg ccacgcaacc accgcttcat ggttgtactc ctgtatcggc ttgactggca
     1261 gcaacagcac ttggcgcttg cttaggttgc agatgatggc gcgccagagt ttccaacaac
     1321 accgcatggg tgctatccaa ttccaagcct tttttggcaa aatcacgcgc cttgcgctct
     1381 tgtcccaaag ccatgtaggt gtcaaccaag tgagccgcca cttctgcatc gggcattttg
     1441 tcgtaggcaa acttcaaata tgccaaggct ttttcaggtt ggcctttaag gtaataagcc
     1501 cagcccaaac tgtcattgat gtgcggggct tgaggttcca gtttgtaggc ttgctcaacc
     1561 aaagcaaacc cttgctcacg ggtcgcttct tgcgacaata aggtataacc cagcacattc
     1621 atggcattgg catcattggg gcgcaaaatc aattgctttt gcaaatcctt caagccatct
     1681 tcaggccgct tcaacttgtc ggtcaataaa ataccgcgct gtgaatacaa ctctgccaac
     1741 aaattcacat ctggcgcgcc actggccaat tgttcatcga tttttttgct gtactcacga
     1801 tacaccacca tcgggtcttg ctgattgtat atccacaaca attccaaacg ctgtgcctga
     1861 ccttggtcgt acaaactgtt ggccgcaggt ttactggctt gtagtttgtc caactgcttg
     1921 cgcatttcgg gccaatcttt gcgctcagcc gcggcagttg ccaaaatcac gcttttatcg
     1981 aattggaatt cggcatcatc gatttttgca gcccattccg cacctttttc agtttggtct
     2041 tggccaaatg ccaccactgc caacaaggtc gccgcacggt tgcgttgtgc cacatcacca
     2101 ccttggtaaa ccttgctcaa aaacacattg gcatcgcgat agtctttctt cttcatcgcc
     2161 aatataccgg cctgtaactg caattcaggc gtgggatttt tactgagctc gttttgaatc
     2221 aactgaaagg ctttgtcgaa ttgctcggtg cgcaccagag tgtcaatttg caactcgcgc
     2281 cacatatcag acaatttgct cgcatcggcc tggctgaaaa actggttaat cacttgcggt
     2341 gatttttgtg ccaacaagcc tatggccaat tgtgtggtgg gtcgtatgtc ggcatccaag
     2401 ctggccaagc gattcaatgc gttcacggcc tgtttgtcat gcctagacaa agccgcatac
     2461 atggcttcaa ccacggcggc ttcagacata ttcgggtatt ttttggtggc agccatcaca
     2521 ttgtcgtaac cttgctgcgc cgcttgtggg ccagccagac tcaattgtga cagcaacaag
     2581 aacactcgtc gtgcctgtgc ttcattggct tgggttaaca ccgcaggaaa atcattcaaa
     2641 gcagtaccaa cattgccacg cgatgcatcc aattcccacg ccatttgttt ttgcaattgg
     2701 ctgggcactg gttccacctt gcgccaacgc tcaagcacca gttgcgcttc attatagcca
     2761 ccaccattga tggcaatttg catggcacgc tcagccaatt caggccgacc tgtgcgctca
     2821 aacatcacca cataagcacc caacgccccg cctgtattgc cttgtagcaa agacaactca
     2881 ctggccaaca tccctgcaaa cgcattggcg tcttgggtga ttttttgcag gcggattttt
     2941 tcatcggccg catcacgcgc tttttgcgct gtgggctttt gcaatttgtc caaactggtg
     3001 ttgatgtctt tggtgagcgg agacacggat tggtctgctg gttttttgat ttcagcttgg
     3061 gccaatgccg gagtcgtggc gatcacggcg atacaggccg ctaagggtaa cagccgttgg
     3121 tgccaacttg ccataaaacg tccatccttg agtcatttca ggttgaggcc aatactttaa
     3181 cacagcacaa tgttagtttg ggtatcccta gtccacactt gaatcacaag ttttcagcct
     3241 catttcttag ccctgagttt gtccagaaag ccctcccatg cctgaattac ccgaagtcga
     3301 aaccacgcgc aacggcattg cgccatttac agaaggtcaa accatagacc atgtggtggt
     3361 gcgccaaagc aaattgcgct ggcccatacc cgaagattta aaccaacaca tagcggcctg
     3421 taagatagtg tcggtgtcgc ggcgcgccaa atacttgctc atccacctgc ctcacggcgt
     3481 catcatggtg catttgggca tgtcgggcag tttgcgtgtg tttgaagccg accttgcacc
     3541 tgaagcaggc aaacacgacc atgtggattt ggtgttgcaa aacggtacgg tactgcgcta
     3601 ccacgaccca cgccgctttg gcgcatggct gtggttcagt ggcgcagtgg aaacccatcc
     3661 tctcatctgt aaattgggtc ctgagccgtt ggaagaagct ttttccgccg aatacttaca
     3721 ggccgcattg agcacacaaa aacgcgcttt gaagctggcc ttgatggaca accatgtggt
     3781 ggtgggcgtg ggcaatatct acgccaatga agttttgttc tgctgcaagt tattgcccac
     3841 cacaat
//
LOCUS       DAOMVK010000186         3825 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_6946_length_3825_cov_2.617772, whole genome shotgun sequence.
ACCESSION   DAOMVK010000186 DAOMVK010000000
VERSION     DAOMVK010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6946_length_3825_cov_2.617772"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1328
                     /locus_tag="PK856_06885"
     CDS             <1..1328
                     /locus_tag="PK856_06885"
                     /inference="COORDINATES: protein motif:HMM:NF015831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HRL02131.1"
                     /translation="KMGDLTIDQAGLVIKNGPSITINGIDAGNKTITNVAPGVNGTDA
                     VNVDQLDDVKNTAGKGWNIQTNGDKATQVKPGDTVDIGTTDGETNIQVSRNGNKIDFE
                     LNKNLDLGSTGSVVMGNTLLNNNGLTINGGPSITQNGIDAGNKTITNVAPGVGLTDAV
                     NLSQLNQAITGAKYAGLRVENGSDIIAPNESLNIIGQGGTTVSYDKNTNTYTVSSKNG
                     GTGTGSMDDWNVTGSDGKGGSVNVPITDGKNVDFAAGSKNVVVTPIQTADGAKVTVDV
                     AQDLNLNSVTTKNADGSSTVVNGNGVTIKGKDGKNGASITQAGIDAGNQKITNVAAGT
                     DATDAVNVSQLNAVSNNINNLSNRINEVEDNANAGTATAIAIANLPQAYEPGARVIAV
                     AAGAYEGETGYAIGYSAISDGGNWIIKASGTGNSQQKFGAGAGVGYRWR"
     gene            complement(1598..2563)
                     /locus_tag="PK856_06890"
     CDS             complement(1598..2563)
                     /locus_tag="PK856_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HRL02132.1"
                     /translation="MIQERQQAFLGGGMTAYITHTACLGHNMGEGHPEGPERLAAIKD
                     RLMAAQVFDFLNQVDAPSVSEAQLARVHSPKYIEYLESVQPSVGTYRVDTDTAMNSGT
                     LDAARHAAGAVVKAVDMVMNGQAPNAFCAIRPPGHHAETRKAMGFCFFNNIAVGAMHA
                     IAEHGIERVAILDFDVHHGNGTEEIFKNDDRVMLLSLFQHPFFPYCGDKPIGNNPNIV
                     NTPMKSGSKGEDVREVVADLWQHKLDAFQPQIIFISAGFDGHLEDDMGNFGLVDADYK
                     WITERIVKWAHTLCEGRIVSVLEGGYELSPLSRSVAVHVQALVDA"
     gene            2786..3454
                     /locus_tag="PK856_06895"
     CDS             2786..3454
                     /locus_tag="PK856_06895"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HRL02133.1"
                     /translation="MKIEIWSDFVCPFCYIGKANLLQALHELNINDAEIVWRSFELDP
                     SFPQSENTSHYQLLQDKYQLDLQAAVDKCAQLSEIGRSCGLDMNFAQAYYGNTFLAHQ
                     LQQLAQAKSAQSAADINQALFQASFTLGLPLNQIDTLVALATEYGLDAEEVGTALESQ
                     QYATQVQVDETAAHELNISGVPFFGFDGRLAVAGAQSIDAFKEVIQHILTHDAADDAK
                     MLRQ"
     gene            3472..>3825
                     /locus_tag="PK856_06900"
     CDS             3472..>3825
                     /locus_tag="PK856_06900"
                     /inference="COORDINATES: protein motif:HMM:NF018485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HRL02134.1"
                     /translation="MGLAAYRSVLIASLSTLLALGLFRFAYSALLPLLIAAQWFDAKT
                     ALYLSTANLCGYLIGALSANPLNQRYNHTQLICVAAFVGSMSLLACAIAHLPIAWYVF
                     WRLLAGITGAWLMVLA"
BASE COUNT         1073 a          912 c          848 g          992 t
ORIGIN      
        1 tgaaaatggg cgatttgacc attgatcaag ctggtttggt catcaaaaat ggtcctagta
       61 tcaccatcaa tggtatcgat gcaggtaaca aaaccattac caacgtggct cctggcgtta
      121 atggtaccga tgcagtcaat gtcgatcagt tggatgatgt gaaaaacacg gctggcaaag
      181 gttggaacat tcaaaccaat ggcgacaaag ccacccaagt gaaacctggt gacactgtag
      241 acattggcac aaccgatggt gaaaccaata ttcaagtcag tcgcaatggc aataaaattg
      301 actttgaatt gaataaaaac ttggatttgg gcagcactgg cagcgttgta atgggcaata
      361 ccttgttgaa caacaatggc ttaaccatta acggtggccc tagtattact caaaatggta
      421 ttgatgcagg caataaaacc attaccaacg tggcaccagg tgtaggttta accgatgctg
      481 tgaacttaag ccagttgaat caagccatca caggtgcgaa atatgccggc ctgagagtag
      541 aaaatggcag tgacatcatt gctccaaatg aaagtttgaa catcattggt caaggtggca
      601 cgactgtaag ctacgataaa aacaccaata cctataccgt ttcctcgaaa aatggtggta
      661 ctggcacagg cagcatggac gattggaatg tcacaggctc agatggtaaa ggcggttctg
      721 tgaacgtacc aatcactgat ggtaagaatg ttgactttgc cgctggcagc aaaaatgtgg
      781 tggttacacc catccaaact gctgatggtg cgaaagtaac agtcgatgtc gcacaagact
      841 tgaacttgaa cagtgtcacc accaaaaatg ccgatggcag cagcactgtt gtcaatggca
      901 atggtgtcac catcaaaggc aaagacggta aaaatggcgc cagcatcaca caagcaggta
      961 tcgatgcagg caatcagaag atcaccaatg ttgcggcggg aactgatgcc accgatgctg
     1021 ttaatgttag ccagttgaat gcagtaagca acaacatcaa caacttaagt aaccgcatca
     1081 atgaggtaga agacaatgcc aatgcaggta cggccactgc catcgccatt gccaacttgc
     1141 ctcaagccta tgaaccaggt gctcgcgtca tcgcggttgc agcgggtgca tacgaaggtg
     1201 aaacaggtta tgcgattggt tactctgcca tctctgatgg tggtaactgg atcatcaaag
     1261 cctctggtac tggtaactcg caacagaaat ttggtgcggg tgcgggtgta ggttaccgct
     1321 ggagataaac tctgtttaaa gtaatccatt aataagcggc ctgtaagcac ttacaggccg
     1381 ctttcttatg tctacaccca gtatgcttat cgcgacaata ttccaacagt cacatttcaa
     1441 tgtgcgattc atgccatgat tttccaacat ggtatgttta agaaaataca accagatgaa
     1501 caagataaaa cagttgctac ccagtcaaaa gcggcctgta aattcataat caaatttaca
     1561 agccgctttt atgatttgga taaagtcgtc tatgtgctta tgcatccacc aaggcttgca
     1621 cgtgcactgc cacactgcgt gacaaaggtg acaactcata acccccttcc aaaacagaca
     1681 cgatacgtcc ttcacacaaa gtatgtgccc atttgacgat gcgctcagta atccatttat
     1741 agtcggcatc gacaagacca aaattaccca tatcgtcttc taaatgccca tcaaagcccg
     1801 cgctgatgaa aataatttgt ggctgaaaag catcgagttt atgctgccac aaatcagcga
     1861 ccacctctct cacgtcttcg cctttgctac cagatttcat cggggtatta acgatattgg
     1921 gattgtttcc aatgggttta tcaccacaat aagggaaaaa tgggtgttga aacaaagaca
     1981 acaacatcac cctatcatcg tttttgaaaa tttcttcggt gccgttaccg tggtgtacgt
     2041 caaaatccaa aatggccacc cgttcaatgc catgttcagc aatggcgtgc atggcgccaa
     2101 cggcaatgtt attgaaaaaa caaaacccca tcgctttacg ggtttccgca tgatggccag
     2161 gaggccgtat tgcacaaaat gcattcggtg cttgcccatt catcaccatg tccacagcct
     2221 taaccaccgc gcctgccgca tgtcttgccg cgtctaaagt gcctgagttc atggcagtat
     2281 cggtatccac gcggtaagtt ccgacggaag gctggaccga ctctaggtac tctatgtatt
     2341 tgggtgagtg cactcgcgcc aattgggctt cactgacgct gggagcatca acctgattga
     2401 ggaagtcaaa cacttgcgcc gccatcaatc ggtctttgat ggctgccaaa cgctcaggcc
     2461 cctctgggtg cccttctccc atattgtgtc ccaaacatgc tgtgtgggta atgtaagccg
     2521 tcattccccc gcccaaaaaa gcttgttgtc tttcttgtat catcagccac gctctcctca
     2581 gtatctctct cacattcata tctttatttt ggtcaattca aacagatgat gtgtggcata
     2641 agtcttctta tcattgtgaa cttatcatac ccgcgtttat cctgactgaa cagaagccat
     2701 actgaatgca gacaaatgcc atctgccatt ttcagctgaa tgtggtatgt tggggtttta
     2761 ctgtatttga gatttgagac gattcatgaa aatcgaaatt tggtctgatt ttgtttgccc
     2821 gttttgttac ataggcaaag ccaatttact acaagcctta cacgaattaa acatcaatga
     2881 tgcagagata gtttggcgca gctttgaact cgatccaagc ttccctcaat ctgaaaacac
     2941 cagtcattac caattgctac aagacaaata ccaattagat ttacaggccg ctgtagacaa
     3001 atgtgcgcaa ttatctgaaa ttggccgcag ctgcggctta gacatgaatt ttgcccaagc
     3061 ctattatggc aatacttttt tggcgcacca attacagcaa ttggcccaag ccaaatctgc
     3121 tcaaagcgcg gcagacatca atcaagcctt attccaagcc tcatttactt tgggattgcc
     3181 tttaaatcaa atcgatacct tggtggcttt ggctaccgaa tacggtttgg atgcagaaga
     3241 agtaggtact gccttagaga gccaacaata cgccacccaa gttcaggtag atgaaactgc
     3301 cgcacatgaa ttaaacatca gtggtgtgcc cttctttggc tttgatggcc gcttggccgt
     3361 cgcaggagca caatccatag acgcattcaa agaagtcatc cagcacattt tgacccatga
     3421 tgccgctgac gatgccaaaa tgttaagaca ataaatcgtt tgaaaggcaa tatgggtcta
     3481 gccgcttacc gcagtgtcct catcgccagc ctcagcacct tattggctct gggcctgttt
     3541 cggtttgcgt attccgccct attgcctttg ttgattgccg cccaatggtt tgatgccaaa
     3601 accgccctgt atttgagcac agccaattta tgcggttatt taattggcgc cttatcggcc
     3661 aacccactga atcagcgata caaccatacc caactcatct gtgtcgcagc ctttgttggc
     3721 agcatgagct tgctcgcctg tgctatcgca catttaccga ttgcatggta tgtgttttgg
     3781 cgcttactgg caggcattac aggggcttgg ttaatggttt tagca
//
LOCUS       DAOMVK010000187         3822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_6955_length_3822_cov_3.328643, whole genome shotgun sequence.
ACCESSION   DAOMVK010000187 DAOMVK010000000
VERSION     DAOMVK010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6955_length_3822_cov_3.328643"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            309..935
                     /locus_tag="PK856_06905"
     CDS             309..935
                     /locus_tag="PK856_06905"
                     /inference="COORDINATES: protein motif:HMM:NF025380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HRL02135.1"
                     /translation="MMKRLGKTLLLLVFVLAATVAAYYAYFMYTAQTQLSQNQLNTVS
                     REGIVTQIHELNRLETMAFHIDTVVKSEKEGTWYKLWQDHEKGLFVIKGQVIAGVDLS
                     KLDASDVTVSADGKQVSIDLPPAEIFTISLADIQMYDYQRGWLNPEPVNPEVLNQVQS
                     VARTQVKQSACKAEILKLATDNASKQVAKLFELTRIKVSVQAGIIPSC"
     gene            1005..2351
                     /locus_tag="PK856_06910"
     CDS             1005..2351
                     /locus_tag="PK856_06910"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRL02136.1"
                     /translation="MLLPDDQPKADLHPDRLRALAILLGLPVPTAVEHSAILVLGVSL
                     GSNLLALACEYPQASFTAMCMNQRELNQLQAACRHLGLHNIQLLLSEDIPAKHETGFD
                     YILCHGYYSYLQEADKEALLHHISLNLNENGITYLDYLIEPGWQTFSTLQHLIANSSH
                     FATQPSLEELHKGIHLIHAQLPEHSPLKTSLEHTVPNLSLRSHPDLNGYWPLQAAFAD
                     TAQSFIQGLHQVALGYVCDSDLSRYYFDHFNPALLQWCGNDFHKKENLYDLLHHQASR
                     ASLIVPIQQLIGFHLPKHALICERMLSLHITGRFELDSAQNAWHCVDAPELTVVASSF
                     NNVLVTAINQAYDADSTVSVSALLEALDHSFKQIPDIKLHATKLVADMVLSGMVHIRS
                     QSQNGFSHRQQMLSLHHWRMQHSPYLTPANKWLQAWLEADLMALCEPSASKTTTGS"
     gene            2420..3514
                     /locus_tag="PK856_06915"
     CDS             2420..3514
                     /locus_tag="PK856_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019389799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HRL02137.1"
                     /translation="MNALLPDTRPFPTDPVKNDLLMYANQMAQNPNNAQGKLAGEQLR
                     NQIATMLFERHILGLSVAMSMAANPAAYQQLWLSLNEVLTAQKDTEVQWLALPVILVA
                     GSKQPMALNNTTPVPALQAFFAQYPQFAALQDATWLPHLVDGSELATIKSDKWFAAKE
                     SAAAAASLVELLPAADLHFTAGQEVVVVYALAYGNQALKSILGTALQEAAMPLMQLWT
                     EHFHGNGVTVFANPMTAMTPMMALQDASALRTRMALDVFAANAIRSIRLQSPRAGVVV
                     AAQAGGKILFGFNATDAVMSLAPQVFIWTLASNDDLDLIMANFIDLMVECQVEHIRVL
                     HEALPEGVELPAYAKAVDMLSSNPLFPQTH"
     gene            3596..>3822
                     /locus_tag="PK856_06920"
     CDS             3596..>3822
                     /locus_tag="PK856_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HRL02138.1"
                     /translation="MQRILFVCLGNICRSPMAEFLLRDMLAQQGLVARISVDSAGTSG
                     WHDGEDMHVGTAKILTDLGIDHTGFHSSKVQA"
BASE COUNT          999 a          971 c          854 g          998 t
ORIGIN      
        1 tgactttgga aagtgctgtt gaattcatcg acgatgatga attggtagaa atcacaccaa
       61 aatcattgcg tttgcgcaaa cgctatttga gcgaaaccga acgtcgccgt catttcaaaa
      121 aattagacta agcagtactc agtcttttga tttgatgtac atgaaagcgt cctttacagg
      181 acgctttttt cattgctgct accaaacttc attcagctta acctttgcat acacacttgt
      241 gacttgcatt ctcgcgccct agcctcattt aaagcttacc gatttttgaa cgcatggatg
      301 actgacagat gatgaaacgc ttgggcaaaa ccttgttact cttggtgttt gtgttggcag
      361 ccactgtggc tgcctattat gcgtatttta tgtacacagc ccaaacccaa ttgtcgcaaa
      421 accagctcaa cacggtcagt cgcgagggca tcgtcaccca aatccatgag ctcaaccgtt
      481 tggagaccat ggcgtttcac attgataccg tggtgaaatc tgaaaaagaa ggcacttggt
      541 acaaattgtg gcaagaccat gaaaaaggct tgtttgtcat caaaggccaa gtgattgctg
      601 gggtagactt atcaaaattg gatgccagtg atgtcaccgt atccgccgat ggcaaacaag
      661 tgtccatcga tttgccccct gctgaaatat tcaccatctc tttggccgac attcaaatgt
      721 acgattacca acgtggctgg cttaatccag aaccagtgaa tcccgaagtt ttgaaccaag
      781 tacaaagcgt ggcacgcact caggtcaaac aatctgcatg taaagccgaa attttgaaat
      841 tagcgactga caatgccagc aaacaagtgg caaaattgtt tgaactcacg aggattaagg
      901 tatccgttca agcgggaatt ataccgtcat gttagtagtt ttgacctaaa caaccgatac
      961 cacctattat cctgtcatta atttacttat tttaacagga taacatgctg cttccagacg
     1021 accagcccaa agccgacttg caccctgatc gcctacgtgc actggccatc ttattgggtc
     1081 tgcccgtacc gacagcggta gaacactcag ccatcttggt tctcggggtg agtttgggca
     1141 gcaacctctt ggccttagcc tgtgaatatc cgcaagcgag tttcacggcc atgtgtatga
     1201 atcaacgtga attaaaccag ttacaggccg cttgtcggca tttgggttta cacaatattc
     1261 aactgcttct cagtgaagat atccctgcca agcatgaaac aggctttgac tatattttgt
     1321 gtcatggcta ttacagctat ttacaagagg ccgataagga agcgctgctg caccacatca
     1381 gcctaaacct taatgaaaat ggcattacct atttggatta tctgattgaa ccaggctggc
     1441 aaacctttag tacactgcaa cacctcatcg ccaacagcag ccattttgcg acccaaccca
     1501 gcttagagga gctgcacaaa ggtatacact taatccatgc ccaattgcct gaacacagcc
     1561 cactcaaaac cagcttagag cataccgttc cgaatttgag tttgcgctca cacccagact
     1621 taaatggcta ctggcctttg caagctgcat ttgctgatac cgcccaaagc tttattcagg
     1681 gtttacacca agtagcctta ggctatgtct gcgattcaga cctttcacgc tattattttg
     1741 accacttcaa ccctgctctg ctgcaatggt gtggcaatga ttttcacaaa aaagaaaacc
     1801 tgtatgactt gctgcaccac caagccagtc gcgccagttt gattgtgccc atacagcaat
     1861 tgattggatt tcacttgccc aagcacgctc tcatttgtga gcgcatgctg agcttgcaca
     1921 ttacaggccg ctttgaactg gactcagccc aaaatgcttg gcattgtgtt gacgctcccg
     1981 agctcactgt ggttgccagc tcatttaata atgtgttggt gaccgccata aaccaagctt
     2041 atgatgcaga cagcaccgtg tccgttagcg cattgttgga ggccttagac cacagcttca
     2101 aacaaattcc cgacatcaag ctccatgcaa ccaaattggt ggccgacatg gtgttatcag
     2161 gcatggtaca catccgcagc caaagccaaa acggattttc acaccgtcag caaatgctgt
     2221 ccttgcacca ttggcgcatg cagcacagtc cttacctcac gcctgccaac aaatggctac
     2281 aggcttggct tgaagcagat ttgatggcct tgtgtgaacc cagcgccagt aaaaccacaa
     2341 ctggttcata gtttacacca atgaacttga cctccagctc tgctaaaata gcagtctatt
     2401 gaacctgtgg aagtttgccg tgaatgcttt gttacccgat acccgccctt ttccaaccga
     2461 ccctgttaaa aacgacttgt tgatgtatgc caatcaaatg gcacaaaatc ccaacaatgc
     2521 ccaaggcaaa ttggcgggtg aacaattgcg caatcaaatc gccaccatgt tgtttgagcg
     2581 ccatatttta ggtctgtctg tggccatgtc catggcagca aatcctgcgg cttaccaaca
     2641 attgtggttg tcattgaatg aagtcttaac cgcacaaaaa gacaccgaag tgcagtggct
     2701 cgccttgcct gtgattttgg tggcaggcag caagcagcct atggcattga acaacactac
     2761 acctgtgcca gctttacagg cattttttgc ccaataccca caatttgctg ctttgcaaga
     2821 cgcgacttgg ttgccacatt tggtcgatgg ctcagaattg gccaccatca aatccgacaa
     2881 atggtttgcc gccaaagaat cagccgctgc agccgcatca ttggtcgagc tgttaccagc
     2941 cgccgatttg cattttactg caggacaaga agtggtggtg gtgtatgcct tggcttatgg
     3001 caatcaagca ttgaaatcca tactaggcac ggccttacaa gaagccgcca tgcctttgat
     3061 gcaattgtgg acagagcatt ttcatggcaa cggcgtgacc gtatttgcca atcccatgac
     3121 cgccatgacg ccgatgatgg ccttacagga cgcctcagct ttgcgtaccc gcatggcttt
     3181 ggatgtgttc gcagccaatg ccatacgctc tattcgtttg caaagcccac gcgctggcgt
     3241 ggtggtcgcg gcgcaagcag gtggcaaaat tttgtttggt ttcaacgcca ccgatgcggt
     3301 catgagcttg gcgccacaag tgtttatttg gactttggcc tcaaacgatg atttggattt
     3361 gattatggcc aacttcattg atttgatggt ggagtgccaa gtagagcaca tccgcgtttt
     3421 gcacgaggct ttgcccgaag gcgtagaatt gccagcttat gccaaggcag tagacatgtt
     3481 gagcagcaat cccctcttcc cacaaacaca ttaagcctgt ttacaggccg cttaggcatt
     3541 gctcacgcat caataagcgg cctgtaaaca ccttctatgt cgcttaagga tgtttatgca
     3601 acgcatctta tttgtgtgct tgggcaatat ttgtcgctca ccgatggctg agtttttact
     3661 gcgtgacatg cttgcccaac aaggcttggt ggcacgcatc agcgtagaca gcgcaggcac
     3721 ttcaggttgg cacgatggtg aagacatgca tgtgggcacg gccaaaattt tgaccgactt
     3781 gggcatagac cacactggtt ttcacagcag caaagtccaa gc
//
LOCUS       DAOMVK010000188         3801 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_7002_length_3801_cov_2.969834, whole genome shotgun sequence.
ACCESSION   DAOMVK010000188 DAOMVK010000000
VERSION     DAOMVK010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7002_length_3801_cov_2.969834"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..1257
                     /locus_tag="PK856_06925"
     CDS             172..1257
                     /locus_tag="PK856_06925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRL02139.1"
                     /translation="MSKQKTVLFLESSKNMGGQEWQLLQQMQNLQAQGYRCVLLCHPQ
                     ARIAQQAQERQLETVYLGFRNSAHLPTIVGIKRAIKQYQPQACICHSGHDANNLSIAC
                     VLLPHRPKIIRSRTYYTNDKKKALQALLPLDVLMVPSRFMQAQIQKQFPSKRVEVVYP
                     GVDFAKLDGQTEADLPEALTHWLAQRPDAEVVMQVGMFRREKGHHIVLEAIAQLLATR
                     PNVVYVMAGGGKSDTLSAHIERLGLQDKVWMGELRAIAPALRQADVMVMPSLLEPLGM
                     VQIEALGLAVPVIVSNVGGIPETVTHQQTGLIVDDMSPEAWAHAIDYALSHPDTMRVW
                     ARAGQTQVRQQFSPEHTTKRLLQLLNA"
     gene            complement(1475..1550)
                     /locus_tag="PK856_06930"
     tRNA            complement(1475..1550)
                     /locus_tag="PK856_06930"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1515..1517),aa:Ile,seq:cat)
     gene            complement(1609..3570)
                     /gene="rpoD"
                     /locus_tag="PK856_06935"
     CDS             complement(1609..3570)
                     /gene="rpoD"
                     /locus_tag="PK856_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HRL02140.1"
                     /translation="MANQNPKKQELEQDTHDDAPLSVEEQRARLRQLIILGKERGYIT
                     YAEINDHLPDDVSDTDQIENIVSMISGLGIQVSEEAPDADSLLMNDGATGVTDEDAVA
                     EAEAALSSVDSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIERALRNMVEAISA
                     CPSTIEEILGLMQRVRDDEIRVDEVVEAIIDPEEEEAALNAIAEEASEAALNEDEEAE
                     AEDDAEEDEDEEVSEEDGAAIASANLEELRQNALSHFEIVAVKFDSMVVVLEKHGSAH
                     PDYVTARQAITEDLLKVRFATRQIESLCESLRQRVNTIRQLERGIRDICVNNVHMPLE
                     YFREHFAPNLVDVNWVENELNRSHKDWNNALERFKFSIMEKQTKLLDMQKLSRLSIEE
                     LKDINKDMARGERESASAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKA
                     VDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQYLQE
                     TGEDPDSAKLAELMEMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAP
                     SDAAMYSGLREVTKEVLESLTPREAKVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQ
                     IEAKALRKLRHPTRSERLKSFLDSEENKQ"
BASE COUNT          948 a          921 c          883 g         1049 t
ORIGIN      
        1 gtgttttggt gtttggccac agtgctgatg gcttcattca gaatgcaaca ggcaataaaa
       61 tgacaaatca acacacagag tgtggtttag cctttaaaat aagccctcct gtcatgtgga
      121 gcacgccaac catggaatgg ccgctccgct tcatcctcac ataggtctta gatgagcaag
      181 caaaaaaccg tgttgttctt agaaagctcc aaaaacatgg gcggccaaga atggcaattg
      241 ctgcaacaaa tgcaaaattt acaagcacaa ggctaccgtt gtgtactttt atgtcaccca
      301 caagcccgca tcgcacagca agcacaagag cggcaattgg aaacggtgta tttgggcttt
      361 agaaacagcg cccatttgcc gaccattgtg ggcatcaagc gggccatcaa acaatatcaa
      421 ccccaagcct gtatttgtca tagcggtcac gatgccaaca atttgagcat tgcttgcgtg
      481 ttgctgcctc atcgccccaa aatcatccgc tcgcgcacct attacaccaa tgacaagaaa
      541 aaagccttac aagctttgtt gcctttggat gtgttgatgg tgcccagccg cttcatgcaa
      601 gcgcagatac agaagcaatt tcccagcaag cgggtagagg tggtgtatcc aggggtggat
      661 tttgccaaat tggatggcca aaccgaggcc gatttgcctg aggctttgac ccattggttg
      721 gcacaacgac ccgatgccga agtggtgatg caagtgggca tgttcaggcg tgaaaaaggc
      781 caccacattg tgttggaggc cattgcccaa ttgttggcga ctcggccaaa tgtggtgtat
      841 gtgatggcag gcggtggcaa atcagatacg ttgagcgcac acattgagcg cttaggtctg
      901 caagacaagg tgtggatggg cgagttgcgt gccatcgctc ccgcgctgcg ccaagccgat
      961 gtgatggtga tgccatcgtt gcttgaacca ctcggtatgg tgcaaattga agcattgggc
     1021 ttggctgtgc cggtgattgt gagcaatgtc ggcggcattc ccgaaaccgt gactcaccag
     1081 caaacaggtc tgatagtgga tgacatgagt ccagaagctt gggcacatgc catcgattac
     1141 gctctcagcc accctgatac catgcgtgta tgggccaggg cgggtcaaac acaagtgcgc
     1201 caacaattct cacctgaaca cacaaccaaa cgtttgctgc aattgttgaa tgcttagtgc
     1261 acgtttaagt atcgtgtagt aagtcataaa agcggcctgt aaatgtttac aggccgcttt
     1321 tatttggtgg tgtattcctg ccatggtttt atcaattaaa accgacaccg tgaaattgtg
     1381 ccccaaacgt ctaacaaacc gtctataagc gagcaacaaa aaagccaccg aattggtggc
     1441 taatctgtaa aaccttgtct agcaaggcta atcttggtgg gcccagagag aatcgaactc
     1501 tcgaccaagg gattatgagt cccctgctct aaccgctgag ctacaggccc taatgaagaa
     1561 gcggaagtat aacaaacatg ccgcttgatt aaaagacctt aaagcagatt attgcttatt
     1621 ttcttcactg tctaagaaac tttttaagcg ttctgagcga gtagggtgac gtaatttacg
     1681 caaggctttg gcttcgattt gacgaatacg ctcacgggtc acatcgaatt gcttgcccac
     1741 ttcttccaaa gtatggtcgg tattcatgtc tatgccaaaa cgcatgcgca agactttggc
     1801 ttcgcgtggg gtcaagcttt ccaaaacctc tttggtcact tcacgcaagc ctgagtacat
     1861 ggccgcatct gatggtgcca cattgttcgc atcttcaatg aagtcaccca aatgtgaatc
     1921 atcatcgtca ccaatcggcg tttccatgga aatcggctct ttggcgattt tcatgatttt
     1981 gcggattttg tcttctggca tttccatcaa ttcggccagc ttggcagaat ccgggtcttc
     2041 gccagtttct tgcaggtatt ggcgcgagat gcggttcatc ttgttgatgg tttcaatcat
     2101 gtgcaccgga atgcggatgg tgcgtgcttg gtcggcaatt gagcgggtaa tggcttggcg
     2161 tatccaccaa gtggcgtaag tcgagaattt gtaaccacgg cggtattcaa atttgtccac
     2221 ggctttcatc aaaccgatgt tgccttcttg aatcaagtcc aagaattgca aaccacggtt
     2281 ggtatatttt ttcgcaattg aaatcaccaa gcgcaagttg gcttgtatca tttcttgttt
     2341 ggcagaagca gattcacgtt cgccacgcgc catgtctttg ttgatgtctt tcaattcttc
     2401 aatcgacaaa cgcgacaatt tttgcatgtc caacaactta gtttgctttt ccatgatgga
     2461 aaacttgaag cgctctaagg cattgttcca gtctttgtgg ctgcggttta attcattttc
     2521 aacccagttg acatcgacta agttaggcgc aaaatgctca cggaaatact ccaaaggcat
     2581 gtgcacattg ttcacgcaaa tatcgcgtat gccacgctcc aattgacgga tggtattgac
     2641 tcgttggcgc aggctttcac acaagctttc aatttgacgg gttgcaaagc gaactttgag
     2701 taaatcttcg gtgatggctt gacgagcggt aacatagtct ggatgagcgc tgccatgctt
     2761 ttctaaaacc accaccatgc tgtcaaactt gaccgccacg atttcaaaat ggctcaaagc
     2821 attttgacgc aactcttcca agttagcgct ggcaatggcg gcgccatctt cttcactgac
     2881 ttcttcgtct tcgtcttctt ctgcatcatc ttcagcctct gcttcttcgt cttcattcaa
     2941 agcagcttct gaggcttctt cagcaatggc atttaaggcc gcttcttctt cctcgggatc
     3001 gatgatggct tcaacaacct cgtcaacacg aatttcatca tcacgaacgc gttgcattag
     3061 gcccaagatt tcctcaatgg tgcttgggca agcgctgatg gcttccacca tattgcgcag
     3121 tgcacgttca atttttttgg caatgatgat ttcgtcttca cgggtcaata agtccacttg
     3181 gcccatttca cgcatgtaca tgcgcacagg atcggtggta cggccaaatt cagaatcaac
     3241 actggataaa gccgcttcgg cttcggccac tgcatcttca tcggtaacgc cagttgcacc
     3301 gtcattcatc aacaaggaat cggcatcagg cgcttcttca gaaacctgta tgcccaagcc
     3361 tgaaatcatg ctgacgatgt tttcaatttg atcggtatca gacacatcat caggtaagtg
     3421 gtcattgatt tcggcatagg taatgtaacc acgctcttta cccaagataa ttaactgacg
     3481 caaacgcgca cgctgctcct ccacactcaa tggagcatcg tcatgagtgt cttgctcaag
     3541 ttcttgtttt ttaggatttt gatttgccat actcttctca aagcttaagg ttttggagga
     3601 ttatcgaagt tgcgtttctt ccgagttgtt tctatagatt tgccattaag gccggtcact
     3661 gatcagtgcc actaacaaac ggttttcatc gctactcaga cctactgtac tgtttttgag
     3721 caccaaatct tggatttgtt gtgcctttaa ttcttttaac aagcgctgca taccgtcctg
     3781 aaaaccttgt tcatcttcag g
//
LOCUS       DAOMVK010000189         3796 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_7009_length_3796_cov_3.219995, whole genome shotgun sequence.
ACCESSION   DAOMVK010000189 DAOMVK010000000
VERSION     DAOMVK010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7009_length_3796_cov_3.219995"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..194
                     /locus_tag="PK856_06940"
     CDS             3..194
                     /locus_tag="PK856_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HRL02141.1"
                     /translation="MYNYQSEFTQFMNDYLEKNPEVAAGRLTNRAKLWDVTLKDEEQS
                     NFEAADLPKPAYTYQTSAD"
     gene            200..877
                     /locus_tag="PK856_06945"
     CDS             200..877
                     /locus_tag="PK856_06945"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HRL02142.1"
                     /translation="MTVETWSIAPELAQYLHAIGPKEPEVLQRLRERNTGHRLGKMSL
                     APEQAQLLVWLAKLTQTKKYLELGVFTGYSSTAMALALPDDAQIIACDLNASFTDLAK
                     ETWQEAGVANKIELRLQPALFSLQELLDEGHAGSFDMAFIDADKAPTPQYVDLCLELV
                     RVGGIVAIDNVLLGGRVLQAAQADSPPSVSILQQFNQAMPNDPRYVAITLPVGDGMTL
                     LVKQTQD"
     gene            881..1603
                     /locus_tag="PK856_06950"
     CDS             881..1603
                     /locus_tag="PK856_06950"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRL02143.1"
                     /translation="MFKCVGLSSVLISVLLSACQSIAPPQAQLTELAALQESADDSVQ
                     NHEPKSASENIPPEAAVNEVEMLQEHVKLLEQNMAQLQEQIQRLTRQQIGLNQSIGVL
                     AKNQGVRTNTVPIDNSVLKADQTYSRAYQQYQQKKYTQAIQTLRPYANGGDGRNDASN
                     GMYLLMLSHKNLKQCESTIAIGRQFQQRFPQHIRAAEALYSVGQCQYDMQQKDIARDT
                     WKRVRVLYPHSAAAKRAAQQSK"
     gene            1617..2108
                     /gene="purE"
                     /locus_tag="PK856_06955"
     CDS             1617..2108
                     /gene="purE"
                     /locus_tag="PK856_06955"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRL02144.1"
                     /translation="MVQVGIIMGSNSDWNVMQHAAAFLQQFGVAFECKVVSAHRTPDL
                     MFEYAEQARGRGLKAIIAGAGGAAHLPGMVAAKTTLPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAISLLANENAELAAQLDAFRQKQTDTVLAMDLPE
                     IDV"
     gene            2160..2777
                     /locus_tag="PK856_06960"
     CDS             2160..2777
                     /locus_tag="PK856_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02145.1"
                     /translation="MQTFATALLTGMTLLLSQAAWAEIAAPAMADNTARIRLFGQNQR
                     PSLAYVGIDCNTNPKGNKINVGGGLGDAFGSFTRTIKNTDLGMAATPATQSLSQRNGI
                     LSKAFYKEIIVPAGLPLNAKAHFIGLSSTNIYQTDSGSSITTYRYTMTEKSTSSKGIS
                     FVPQAGHDYEILSLREQGKEYLVVTDITTPDFEAVAVNEPFSCKK"
     gene            2781..>3796
                     /locus_tag="PK856_06965"
     CDS             2781..>3796
                     /locus_tag="PK856_06965"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HRL02146.1"
                     /translation="MTVLSTPILPPARLGILGGGQLGRMFTVAAKTMGYQVVVLDPDP
                     LAPAAEYADEHLCAPFTDATALAKLAQCAAITTEFENVNADAMRQLAAHTVVSPSGDC
                     VAIAQNRIHEKAWIRKAGLQTAPYQVIEQAADITEASAEFLPGILKTATMGYDGKGQI
                     RVKTVAELQAAFAEHNGVACVLEKMVDLRAEISVIVCRLNNDQAVCFDPAENQHIDGI
                     LAYSIVPARLPHALQQQAQTMAKRLADELDYVGVLAVEMFVTGDEQVLVVNEMAPRPH
                     NSGHHTLDACITDQFQQQVRLMCGLTPGNTTLLVPCTMANILGDVWGEDGSEPNWLNL
                     QQQA"
BASE COUNT         1054 a         1009 c          878 g          855 t
ORIGIN      
        1 ctatgtacaa ctaccaatca gaattcaccc aattcatgaa tgactatctg gaaaagaatc
       61 cagaagtggc agcgggacgt ttgaccaacc gcgccaaatt gtgggatgtc actttaaaag
      121 acgaagaaca aagcaatttt gaagcagccg acttgcctaa acctgcttat acctaccaaa
      181 ccagcgctga ctgattgtag tgacggtaga aacatggagt attgcgcccg agttggcgca
      241 atatttacac gcaataggcc ccaaagagcc ggaagtttta cagcgcttgc gtgaacgcaa
      301 tacaggtcac cgtttaggca aaatgagttt ggcacccgag caagcgcaat tattggtgtg
      361 gctggccaaa ctcacgcaaa ccaaaaaata cttggaattg ggtgttttca caggttacag
      421 cagcacagcc atggctttgg ccttgcccga tgatgcccaa atcattgcct gtgatttaaa
      481 tgccagcttt actgacttgg caaaagaaac ttggcaagaa gcaggtgtcg caaacaaaat
      541 cgaattgcgt ttgcaacctg ctctttttag cctgcaagaa ttattggatg aagggcatgc
      601 gggcagtttt gacatggctt tcattgatgc cgataaagcg cccaccccac aatatgtgga
      661 tttgtgtttg gaattggtgc gcgtaggtgg gattgtggcg atagacaatg tattgttagg
      721 cggcagggtg ttacaggccg ctcaagcaga cagccccccc agtgtcagca tcttacagca
      781 gtttaatcag gccatgccaa acgacccacg ttatgtggcc attaccttac ctgttggtga
      841 tggcatgact ttgttggtca aacaaactca ggattgaatc atgttcaaat gtgttggttt
      901 aagcagcgtg ctcatcagtg tgttactcag cgcatgtcag agcatcgccc ctcctcaagc
      961 acagttgaca gaactagctg cattgcaaga atctgcggat gattcggtac aaaatcacga
     1021 gcctaaatca gcctctgaaa acatcccacc agaagccgcg gtcaacgaag tggaaatgct
     1081 gcaagaacat gtgaagcttt tggagcaaaa catggcacaa ttacaagagc aaatacaacg
     1141 cttgacccgc caacaaatcg gcctgaatca aagcataggg gtgttggcca aaaaccaagg
     1201 cgtgcgcacc aatactgtcc ccatagacaa ctccgtctta aaagccgacc aaacctacag
     1261 ccgtgcttac caacaatacc aacaaaaaaa atacacccaa gccatacaaa ctttgcgacc
     1321 gtatgccaat ggcggcgatg gcagaaatga tgccagcaac ggcatgtatt tattgatgct
     1381 ctcacacaaa aacctcaaac agtgtgaatc caccatcgcc attggccgac aatttcaaca
     1441 acgttttcca caacacatcc gtgcagcaga agccttgtac agtgtaggcc aatgccaata
     1501 cgacatgcag caaaaagaca ttgctcgcga cacttggaaa cgtgtgcgcg tcctctatcc
     1561 tcacagtgct gccgccaaac gcgccgcaca acaatcgaaa taacaggaat attattatgg
     1621 tacaagtggg catcatcatg ggcagcaaca gtgactggaa cgtcatgcaa catgccgccg
     1681 ccttcttaca acaatttggc gtggcgtttg aatgcaaagt cgtgtcggcc caccgcaccc
     1741 cagacctgat gtttgaatac gccgagcaag ctcgtggccg cggcctgaaa gccattattg
     1801 ctggcgctgg tggcgcagcc cacttgcctg gcatggttgc cgccaaaacc actctgccag
     1861 tattgggcgt tccggtgcca agcaaatact tgcgcggcga agactcgttg ttgtccatcg
     1921 tgcaaatgcc caaaggtgtt cctgtggcga ctttcgctat tggtgaagca ggtgccgcca
     1981 atgccgcctt gtttgccatt tccttgctag ccaacgaaaa cgccgaattg gcagcacaat
     2041 tggacgcatt ccgccaaaaa caaaccgaca ccgttttggc gatggacttg cctgagattg
     2101 atgtttaaaa cattcaaaac atttacagac cgcttatgaa gcgtatttta ggaaatcaca
     2161 tgcaaacctt cgccaccgcc ttattaactg gcatgacgct gttgctcagc caagccgctt
     2221 gggcagagat tgccgcgccc gccatggccg acaatactgc ccgcatacgc ttgtttggcc
     2281 aaaatcaacg ccctagcctt gcgtatgtgg gcattgattg caacaccaat cccaaaggca
     2341 acaaaatcaa tgttggtggc ggccttggcg acgcttttgg ttcattcacc cgcaccatca
     2401 aaaacaccga cttaggcatg gcagccacgc ccgccacaca aagcttaagc caacgcaacg
     2461 gcattctttc taaggcattt tacaaagaaa tcattgtccc cgcgggtttg cctttgaacg
     2521 ccaaagccca tttcattggc ttgagctcaa caaacatata ccaaactgac tcaggctcaa
     2581 gcatcaccac ttaccgatac accatgacag aaaagtccac ttcttccaaa ggcatttcct
     2641 ttgttcctca agctggacat gattatgaaa tcctgtcatt gcgtgaacaa ggcaaagagt
     2701 atttggtggt caccgacatt accacacccg attttgaagc agtcgcagtt aacgaacctt
     2761 tttcttgtaa aaaataaacc atgacagtct taagcacacc catattacct cccgcccgtt
     2821 tgggcatctt aggcggcggc caattgggcc gcatgttcac tgtggcagcc aaaaccatgg
     2881 gctaccaagt ggtcgtgcta gaccctgatc cactggcacc tgctgctgaa tatgctgatg
     2941 agcatttgtg cgccccgttt accgatgcaa ctgccttggc caaattggca caatgcgcag
     3001 ccattaccac cgagtttgaa aacgtcaatg ccgatgccat gcgccaattg gctgcgcata
     3061 cagtggtttc acccagcggg gactgcgtcg ctatcgcaca aaatcgcatc catgaaaaag
     3121 catggatacg caaagcaggc ttgcaaactg ccccttatca agtcatagaa caagctgccg
     3181 acattactga agcctcagcc gaatttttac caggcatttt gaaaaccgcc accatgggct
     3241 acgatggcaa aggccaaatt cgcgtcaaaa ccgtggccga gttacaggcc gcttttgctg
     3301 aacacaatgg tgtagcctgt gtgttggaaa aaatggtgga cttgcgcgcc gagatttcgg
     3361 tcatcgtgtg ccgcttaaat aacgaccaag ccgtgtgttt cgaccctgct gaaaaccaac
     3421 acatagatgg cattttggcc tattccattg tgcctgcccg tttgccacat gccttgcaac
     3481 agcaagctca aaccatggcc aaacgcttgg cagatgaatt ggattatgtc ggcgtgttgg
     3541 cagtggaaat gtttgtgaca ggcgatgaac aagtgctggt ggtgaatgaa atggcaccac
     3601 gtccacacaa ctcaggccac cacaccttag atgcctgcat caccgaccaa ttccagcaac
     3661 aagtgcgctt gatgtgcggc ttaacgccag gcaacaccac cttgctcgta ccttgcacca
     3721 tggcaaatat tttgggcgac gtgtggggcg aagatggcag cgaaccaaac tggctcaatc
     3781 tgcaacaaca agccag
//
LOCUS       DAOMVK010000190         3793 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_7017_length_3793_cov_2.650080, whole genome shotgun sequence.
ACCESSION   DAOMVK010000190 DAOMVK010000000
VERSION     DAOMVK010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7017_length_3793_cov_2.650080"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /gene="glyQ"
                     /locus_tag="PK856_06970"
     CDS             complement(<1..846)
                     /gene="glyQ"
                     /locus_tag="PK856_06970"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HRL02147.1"
                     /translation="MLTFQEIIFKLQTFWAAQGCAVLQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPDNIQELYLQSLQELGL
                     SPLEHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWTKTLDGQVLTYGDVYHQNEVEQSTYNFEYSDTDWLLGQ
                     FNKFEEQAQRILAVEETSLALPAYELVLKAGHTFNLLDARGAISVTERATYIGRVRAL
                     SRAVAQ"
     gene            1159..1359
                     /locus_tag="PK856_06975"
     CDS             1159..1359
                     /locus_tag="PK856_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02148.1"
                     /translation="MNNDDEDNYTQRNLLIFIGVLVGLVTTAIVVWAAWLGLNGGAAA
                     EASAPAAASAVAAAKSWLTMAW"
     gene            1338..1652
                     /locus_tag="PK856_06980"
     CDS             1338..1652
                     /locus_tag="PK856_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRL02149.1"
                     /translation="MVDNGVVKFYFAVGKSDLATGADEALKDVIAGVKEGKKAMVSGY
                     HDSTGTAAVNEEVAKKRAFAVKDALVRLGVTEDQIELVKPQVSEGTGANAEARRVEVV
                     LK"
     gene            1771..2247
                     /locus_tag="PK856_06985"
     CDS             1771..2247
                     /locus_tag="PK856_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02150.1"
                     /translation="MNQLKTLCFAALLASSAWAQAQTWQICTWDVEVVQNAKSWQSVD
                     LVEQTKFNSGEVAAIEIDNERRGWEFTGRFLRTNGKYTEPCTYVNGKTQRMVVWPSYE
                     HADKMPPKDVLFQSGDVLTITQYVTEADVAKRQLSSDEFELQAVELNSGVYSREVP"
     gene            2542..3171
                     /locus_tag="PK856_06990"
     CDS             2542..3171
                     /locus_tag="PK856_06990"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HRL02151.1"
                     /translation="METLMAVLLALVLLEISPGPDMMLTLARGIGQGRRIALLSVLGM
                     TLVAGVVQVSLLVLGLATVLHRYPFALQLLQWAGALYLLYLGAKMLYASRAGNTAIAS
                     AAPISGVQAVKEGTINSLTNPKSLLFMFAFIPQFVDPQAGPVWSQLLILGSLQKLTGI
                     VSLGSIALASGKVGECLGKHPRWLLWQQRFTGAVMMGLGVRLFFSGNSK"
     gene            complement(3252..>3793)
                     /locus_tag="PK856_06995"
     CDS             complement(3252..>3793)
                     /locus_tag="PK856_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRL02152.1"
                     /translation="RPEHVGRYPHMFSGGQRQRIALARAMMLNPKIIVADEPTSALDV
                     SIQAQVLNLFMDLQDAYHTAYVFISHNLSVVRHVADDLMVMYLGKAVEHGPKEAIFEK
                     PCHPYTRALLAAAPSLNGHQAELKLKGELPSPLNLPSGCSLHLRCPFAQEQCGQQEPE
                     LRPWQGRLVACHRLEDIAA"
BASE COUNT          915 a          868 c         1107 g          903 t
ORIGIN      
        1 ttgtgccaca gcgcggctga gcgcacgaac acggccgatg taagtggcgc gttcggttac
       61 cgaaatcgca ccgcgggcat cgagcaagtt gaatgtgtgg ccggctttga gcaccaattc
      121 gtaggcaggc aaggccaaag aggtttcttc taccgccaaa atgcgctggg cttgttcttc
      181 aaatttgttg aattggccga gcaaccagtc ggtgtctgag tattcgaaat tgtaagtgga
      241 ttgttccact tcgttttggt ggtaaacatc gccataagtg agcacttggc cgtctaaggt
      301 tttggtccac accaagtcgt acacgttttc cacgccttgc aaatacatgg ccaaacgctc
      361 gatgccgtaa gtgatttcgc ccaatacagg ggtgcaatca atgccgccca cttgttggaa
      421 ataggtgaat tgggtcactt ccatgccatt gagccacact tcccagccca agccccaagc
      481 gcccaaagtt gggttttccc agtcgtcttc gacaaaacga atgtcgtgtt ccaatggcga
      541 caagcccaat tcttgcaagg attgcaaata caattcttgg atgttgtcgg gtgcgggttt
      601 taaggccact tggaattggt aatagtgttg caagcggttg gggttgtcgc cataacggcc
      661 gtctttgggg cgacgcgaag gttgtacata ggcggcaaac caaggctcag ggcccaatgc
      721 gcgcaagcaa gtggcaggat gcgatgtgcc tgcgcccact tccatatcga aaggttgtaa
      781 cactgcacag ccttgtgctg cccagaaagt ttgtaatttg aaaatgattt cttgaaaggt
      841 caacatgaga actaacttta gacgtagccg taaatagggc ttattctact gtatcgaggc
      901 ggtggtgggt agtgagttgc ctgttgcaag agctaagttg tgagggctgg gcaagggcgc
      961 agcttgatga gcagcgtatg gaaacctgtc atgcaagtgt ttacaggccg catacccgtt
     1021 gggtgagaca gaatttgcag caaacatgcc acataaacat gttcaaaaca cagtgaaatc
     1081 agtataatgc gtgcgtttga gtttacatga tgctgcgatg cggcattgct ttgatagaaa
     1141 cggggataca aagtgtcaat gaacaatgat gatgaagata actacaccca acgcaacttg
     1201 ctgattttta tcggcgtgtt ggttggtttg gtcaccactg ccatcgtggt atgggcagct
     1261 tggttaggtt tgaatggtgg cgcggctgct gaagcatctg cgccagcggc agcttctgct
     1321 gtggcggccg caaagtcgtg gttgacaatg gcgtggtgaa attctacttc gcagtgggca
     1381 aatctgattt ggctactggc gcggatgaag ccttgaaaga cgtgattgct ggcgtgaaag
     1441 aaggcaaaaa agccatggtt tcaggctacc atgacagcac aggcactgcg gctgtgaacg
     1501 aagaagtggc gaaaaaacgc gcgtttgctg ttaaagatgc cttggtacgt ttgggcgtga
     1561 ccgaagacca aatcgaattg gtgaaaccac aagtgtctga aggcacgggt gccaatgccg
     1621 aagcgcgtcg cgtggaagtg gtattgaaat aattgctgct tcttgctgct aggcagcaag
     1681 aagccgtctt aaaaaagcac ttgtcagcgc aagtgctttt tttgtgcgcc acattcaggc
     1741 atgatttggc caatgacaaa aggatgacac atgaatcaac tcaaaaccct gtgtttcgcc
     1801 gccttgctcg ccagcagtgc gtgggcgcaa gcgcaaacct ggcaaatatg cacttgggac
     1861 gtggaagtgg tgcagaatgc caaatcttgg caaagcgtcg atttggtaga gcaaaccaag
     1921 ttcaacagcg gtgaagtggc cgcaatagaa atagacaatg aacgccgtgg ctgggagttt
     1981 acaggccgct tcttgcgcac caacggcaag tataccgagc cttgcactta tgtgaatggc
     2041 aaaacccaac gcatggtggt gtggccttca tatgaacacg ccgacaaaat gccgccaaaa
     2101 gatgtgttgt ttcaaagcgg cgatgtgctc accatcaccc aatacgtcac cgaggccgat
     2161 gtggccaagc gccaactgag cagcgatgaa tttgaattgc aagcggtaga gctgaacagc
     2221 ggcgtgtaca gccgcgaagt gccttgatgg tttaacactg acgacatgaa gtattgagcc
     2281 tgtggcaatc tcacagagca tgaacaagcg gcctgtaagt gtttacaggc cgcttgtttt
     2341 tacatcaatc gatatggctt caatctctgg cttaaagagg caagtaagca gcgcaattca
     2401 gcctgattgt cattgaaacc atacaagcct gtggcgcaat cgtgtgtgca aagcgcataa
     2461 tcaagcttac ctgcgcaagg gtgtgggttt acgcgtgaaa atggtcaatc tgttgaagaa
     2521 tcggagccta aaatgcccag tatggaaact ttgatggcgg tgttgttggc gttggtgttg
     2581 ctggagattt cgccaggtcc agacatgatg ctcaccttgg cgcgcggcat aggccaaggg
     2641 cggcgcattg cgctgctcag cgtgttgggc atgacgctgg tggcgggcgt ggtgcaagtg
     2701 tctttgttgg tgctcggctt ggcgacggtg ttgcaccgct acccgtttgc cttgcaactg
     2761 ttgcaatggg caggcgcgct gtatttgctg tatttgggtg cgaaaatgct gtatgcgagc
     2821 agggcgggaa atacggcgat tgcctctgct gcgcccatca gcggcgtaca agcggtgaaa
     2881 gaaggcacga ttaacagctt gaccaatccc aaatcgctgc tgtttatgtt tgcgttcatt
     2941 ccgcaatttg tggatccgca agcagggcca gtgtggtcgc aattgctgat attgggcagc
     3001 ttgcaaaagc tcacgggcat cgtgtctttg ggcagcattg ctttggcttc gggcaaggtg
     3061 ggtgagtgtt tgggcaaaca cccgcgctgg ctgttgtggc aacagcgctt tacaggtgca
     3121 gtaatgatgg gcttgggtgt gcgtctgttt ttcagtggca acagcaagta aacaatgtgt
     3181 ttgaacaacg aataagcggc ctgtaaatgt ttacaggccg cttttacatg cgcttgaatc
     3241 aagtgtaagg cttaggctgc gatgtcttcc aaacggtgac aggcaaccaa gcggccttgc
     3301 cacgggcgca gctcaggctc ttgttggcca cattgttctt gcgcaaacgg gcagcgcaaa
     3361 tgcaatgagc agccgctggg cagattcaag gggctgggca attcgccttt gagcttgagt
     3421 tcggcttggt ggccgttcag gctcggtgcg gcggcgagca gggcgcgggt ataaggatgg
     3481 caaggctttt caaaaatggc ttccttgggg ccgtgttcca ccgctttgcc caaatacatc
     3541 accatcaaat catcggccac atggcgcacc accgacagat tgtgcgaaat aaacacataa
     3601 gccgtgtggt aagcatcttg caaatccatg aacaggttca agacttgcgc ctgtatcgac
     3661 acgtccaagg ctgaggtggg ttcgtctgcg acaatgattt tggggttcag catcatggcg
     3721 cgcgccaagg cgatgcgctg gcgctgaccg ccagaaaaca tgtgcggata gcggccaaca
     3781 tgctcaggcc gca
//
LOCUS       DAOMVK010000191         3767 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_7080_length_3767_cov_2.652209, whole genome shotgun sequence.
ACCESSION   DAOMVK010000191 DAOMVK010000000
VERSION     DAOMVK010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7080_length_3767_cov_2.652209"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(245..721)
                     /gene="lysM"
                     /locus_tag="PK856_07000"
     CDS             complement(245..721)
                     /gene="lysM"
                     /locus_tag="PK856_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HRL02153.1"
                     /translation="MGLFSFIKNAGEKLFGKNEEEVQKEAAANLPDLNRKAADAIKAH
                     IDAQNLGLEGLAVTYDGASGQVTLSGNAPSQEAAEKAGLTAGNVTGVGHVDNNLAAPA
                     AEESQFHDVVSGDTLSAIAKKYYGNANDYMKIFEANKPMLSHPDKIYVGQKLRIPK"
     gene            917..1846
                     /gene="thrB"
                     /locus_tag="PK856_07005"
     CDS             917..1846
                     /gene="thrB"
                     /locus_tag="PK856_07005"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HRL02154.1"
                     /translation="MSVYTSVSEAQMRLFLQQYDLGEFISLKGIAQGVTNTNYFVTTT
                     QGKFVLTLFEVLRQEELPFYLHLMLHLSENGVACPKPVAQLTGRLDAEIAGKPACLVT
                     CLHGADIAHPTAQQCHSVGAMLAKMHVASLSFDAQMANPRGKAWWTAEIERLLPLMSY
                     VDATLLKDTVAALNAHDDSDLPQGIIHADLFKDNVLIHGDDVAGFIDFYYACQGNFMY
                     DLAIAINDWARTDANYIDKEFADALMSGYQSVRPLSEAEQAYLPLAQQAACIRFWVSR
                     LLDFHFPPEGELTFTKNPDVFRDLLLKLQQDQQ"
     gene            1872..2951
                     /locus_tag="PK856_07010"
     CDS             1872..2951
                     /locus_tag="PK856_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02155.1"
                     /translation="MSSCRCAYVVPASYAKNIKIDTKQDIVLPEDANLWAQFHDLRPR
                     FVHYVGNNPAIFDFVKQTQALCLRWQPQSSMNIEINGLNQLILQLVLTEPLRLRCNDD
                     SFREIHLSLGEDFFMPHFGLGLPHMVIKADARTGRIPPLPALYGASHLSINHPWNAKS
                     QANDVAFDCRQLKPYAHLNHLKLNGNMQMWSALTQLPLHKLEVFNAPNLSEFPLLTDM
                     PNLQELTVAGSETEGSKHINYLSKQKQDLVVYFQGTVDRNAWQQAGYVYQQHDNQTAQ
                     SENPVWAQSMAPVDLSDVAWNEEASAADANNPTLFERMRTESVTEFFGFKPKHGGEKT
                     EEQEPSLLERMRTESVGDFFRNKRK"
     gene            complement(3109..>3767)
                     /locus_tag="PK856_07015"
     CDS             complement(3109..>3767)
                     /locus_tag="PK856_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02156.1"
                     /translation="VPQALPDHTDAALSQMQYAAGQLPRHDWAAWFKNLLVYGAILGL
                     SLAVSQGLQDYVRLVVINMDSNTDRMTHVNQVLAIMTTGGLALGLFVSGWVMQFLGRL
                     MALWGLCILALGCMAYMTWQPSMFQDFNGAWVLMGAYLGLGSLMWWSMALVAASVAHH
                     RCTAWALVSVGGVMMAASIHHQLPDLAQGERFAFEYYAAMMVMIAVLIALVRKTQVMD
                     "
BASE COUNT         1018 a          988 c          793 g          968 t
ORIGIN      
        1 gaaaataaat ttctgtaaac gcgcaatttt aaacgctaaa ccgatttccg acaagcgcta
       61 attccatgat gacagactgc gttgccgctt tgcccacaca tcagcccgaa aaacagactt
      121 attgatgctg tttggcagtg cattttgtat tttctaaagt ttgtgtcgtt cacagtcatg
      181 tcatccaatg taaaagcggc ctgtaaacat ttacaggccg ctttcattca aactctagca
      241 acacttattt aggaatgcgc aatttttgac ccacatagat tttatcaggg tgtgacaaca
      301 tgggtttgtt ggcttcaaaa attttcatgt agtcgttggc attgccgtag tattttttgg
      361 caatggcaga caaggtatcg ccagacacca catcatgaaa ttgcgactct tctgcagcag
      421 gtgccgccaa attgttatcc acatggccca cgcctgtcac attgcctgcg gtcaaacccg
      481 ctttttcagc ggcttcttgg ctgggcgcgt tgcccgataa agtcacttgg ccactggcac
      541 catcgtatgt aactgccaaa ccttccaaac ccaaattttg tgcatcaatg tgtgctttga
      601 tggcatcggc agctttgcga ttgaggtcag gcaaattggc agcagcttct ttttgcactt
      661 cttcttcgtt tttaccgaac agcttttcac ccgcattttt aatgaaactg aacaatccca
      721 tattcctact ccttagattt agtcaaacac acgaagtcct ttagaaagtg caagctgcac
      781 aaagttcatc agggccactc aaaaatgaac attatcatgt tttctgataa ttcgcatgat
      841 ttcatcatat gcaatgttgg caagtgtgaa ttttttgtta tcatcctgct tttggttgct
      901 ttgtggtctg tgtcttatgt ctgtttatac ctcggtgagt gaagcgcaaa tgcgcttgtt
      961 tttgcaacaa tatgatttgg gggaattcat cagcctaaaa ggcatcgccc aaggggtgac
     1021 caacaccaat tattttgtca ccaccaccca aggcaaattt gtcttaaccc tatttgaagt
     1081 gttgcgccaa gaagaattgc ccttttattt gcacttgatg ttgcatttaa gtgaaaacgg
     1141 cgtcgcctgt cccaagccag tggcgcaact tacaggccgt ttggatgccg aaatcgctgg
     1201 caaacccgcc tgtttggtga cttgtttaca cggtgctgac attgcccacc ccacggcaca
     1261 acaatgccac tcagtaggcg ccatgttggc aaaaatgcac gtggcaagct tgagtttcga
     1321 tgctcaaatg gccaaccccc gtggcaaagc ttggtggacg gcggaaattg aacgcttgct
     1381 gccattgatg agttatgtgg atgccacttt actcaaagac acggttgccg ccttgaatgc
     1441 ccacgacgac agcgacctgc cccaaggcat cattcatgcc gatttgttca aagacaatgt
     1501 gttgatacac ggcgatgatg ttgcgggctt catcgacttt tactatgcct gccaaggcaa
     1561 tttcatgtac gacttggcca tcgccatcaa tgattgggcg cgcaccgatg ccaactacat
     1621 cgacaaagag tttgcagacg ctttaatgtc aggctaccag agcgtgcgac cactgagtga
     1681 agctgaacaa gcttatttgc ctttggcaca acaagcggcc tgtatccgtt tctgggtgtc
     1741 gcgtttgttg gacttccatt tcccacctga aggcgaactg acgttcacca aaaacccaga
     1801 tgtcttccgt gatttgctgt taaagcttca acaagaccaa caataacaat ataaggtata
     1861 aggttgaacc catgagctct tgtcgttgcg cctatgtcgt gcccgccagc tatgctaaaa
     1921 acataaaaat tgataccaaa caagacattg tgctccccga agatgccaat ctttgggcac
     1981 aattccatga tttacgcccc cgttttgtcc actatgtggg caacaaccca gccatttttg
     2041 atttcgtgaa acaaactcag gctttgtgcc tgcgttggca acctcaaagc agcatgaata
     2101 ttgaaatcaa tggcctcaat caactgattt tacaattggt gctgaccgaa ccattgcgcc
     2161 tgcgctgcaa tgacgattcc tttcgtgaga ttcatttaag tttgggtgag gacttcttca
     2221 tgccccactt tggccttggt ttgccgcaca tggtcatcaa agccgatgcc cgtacaggcc
     2281 gcataccgcc cttgcccgct ttgtatggtg caagccattt gagcatcaac cacccttgga
     2341 atgccaaatc acaagccaat gatgtggctt ttgattgccg ccaactcaaa ccgtacgcgc
     2401 atctaaacca tttgaaactc aatggcaata tgcaaatgtg gtcagcccta actcagcttc
     2461 cattgcacaa attggaggtt ttcaatgcgc ccaatttaag cgagtttccc ctgttaaccg
     2521 acatgcccaa cttgcaagaa ctcacggtgg cgggttcaga aaccgaaggc agcaaacaca
     2581 tcaattattt gtccaaacaa aagcaagatt tggtggtgta tttccaaggc accgttgaca
     2641 gaaacgcctg gcaacaagca ggttatgtct accaacaaca cgataatcaa acagcgcaaa
     2701 gtgaaaatcc cgtatgggca caatccatgg cacctgttga tttgtcagat gtggcatgga
     2761 atgaagaggc atcagctgct gacgccaaca acccgacatt gtttgagcgc atgcgcaccg
     2821 aatcagtaac agagtttttt ggcttcaaac ccaaacatgg cggagaaaaa accgaagaac
     2881 aagaaccctc attgcttgaa cgcatgcgta ccgaatcggt aggcgatttt ttccgcaaca
     2941 agcgcaagtg agctcaacaa ggaaacgagc attgtctgtg agctgttaaa gccatgcgta
     3001 cagccatttc atcatactgg tgtcagtcta tagtgtgaca accacacaaa ctgctctgcc
     3061 atttaataag cggcctgtaa atgcttacag gccgcttatt cttattcatc aatccataac
     3121 ttgggttttg cgaaccaagg cgattaagac cgcaatcatc accatcattg ccgcgtaata
     3181 ctcaaaagca aagcgctcac cctgcgctaa atctggaagt tgatggtgta tgctggccgc
     3241 catcatcaca cctcccacac tcaccaacgc ccaagccgta cagcggtgat gggcaaccga
     3301 agccgccacc aaggccatgc tccaccacat caagcttccc aagcccaaat acgctcccat
     3361 caatacccat gccccattaa aatcttgaaa catactgggc tgccaagtca tgtaagccat
     3421 acatccaaga gccaaaatac ataaacccca taaggccatc agcctaccca gaaactgcat
     3481 gacccacccg ctgacaaaca atcccagcgc caagcccccc gtagtcatga tggccagtac
     3541 ttggttaacg tgcgtcattc tgtcggtatt tgagtccatg tttatgacta ccaaacgcac
     3601 ataatcctgt aaaccctgcg ataccgccaa acttaagccc agtatcgcgc cgtatacaag
     3661 caaattttta aaccacgcag cccaatcgtg acgcggcaat tgccccgccg catattgcat
     3721 ctggcttaaa gcggcatccg tatggtcagg caaagcctgg ggcacca
//
LOCUS       DAOMVK010000192         3741 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_7156_length_3741_cov_2.471243, whole genome shotgun sequence.
ACCESSION   DAOMVK010000192 DAOMVK010000000
VERSION     DAOMVK010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7156_length_3741_cov_2.471243"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PK856_07020"
     CDS             <1..528
                     /locus_tag="PK856_07020"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dimethylallyltransferase"
                     /protein_id="HRL02157.1"
                     /translation="GLYQRLLQVDPVTAARLSANDSQRIERALEIHALTGQAMSELLQ
                     AQNEANHIALETATLIPQQRAQLHHSIAERYDEMLQAGFVDEVQALQQNYPELHADLP
                     SMRCVGYRQVWDYLAGVSSADEMRELGIIATRQLAKRQLTWLRKIAATHAFDPYEHPD
                     LLPQVLPLIRQHFEF"
     gene            530..1048
                     /locus_tag="PK856_07025"
     CDS             530..1048
                     /locus_tag="PK856_07025"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRL02158.1"
                     /translation="MTNAAQPALLKRFLALVYEGLIFLAVTILAIIVALPVSYLLRSQ
                     PMLQQIVITLWFFMAWWLYAKLNWRKGQTLAMKAWHLQLRNELGHIPALQQLRMRFLW
                     AVLFLVLVPVLSYLVLSHFSQLPPKPRFYLSLVWWLLPWGFACVHPSKQFLYDVLAGT
                     RIISKNAAEQSS"
     gene            1112..1972
                     /locus_tag="PK856_07030"
     CDS             1112..1972
                     /locus_tag="PK856_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HRL02159.1"
                     /translation="MRWQGRKQSDNIEDRRGSGGGGGFPGGRGGMGIGGIIIVLVGYF
                     VFGVDLTGMVGGNTAPSAQVANQPAGPVKQTGEEAQLRELSSVVLASTEESWASYFQK
                     HGAIYREPKMVLYRGATSTACGTGQSAMGPFYCPADEKVYLDLAFYDDMKNQLGAEGD
                     FAFAYVIAHEVGHHVQNLLGINQKVSQAQRSGSKKQANQLSVALELQADCFAGVWGQY
                     MQKQGLLEMGDVEKAMNAAAAVGDDRLQQQATGRIVPDSFTHGSSQQRMQWFNRGFQS
                     GDPEQCNTFG"
     gene            1991..2503
                     /locus_tag="PK856_07035"
     CDS             1991..2503
                     /locus_tag="PK856_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NemA protein"
                     /protein_id="HRL02160.1"
                     /translation="MKTLRLYTLTVTAALTLSACGSMGIGFGLGNSGGGVNFGTGISF
                     PLGGLGVRNDPAKSGINLIDVQVVSYFGTAAQGYKSSDNPIDGGFYRRLLGKQGKLWL
                     VQDYYNDSDRKYNEPMLLSVDEAYRFTALPASGTFYTYHENGQIASKRVYENTRLLKS
                     EQWDNSGHAL"
     gene            2578..3672
                     /gene="aroC"
                     /locus_tag="PK856_07040"
     CDS             2578..3672
                     /gene="aroC"
                     /locus_tag="PK856_07040"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRL02161.1"
                     /translation="MPGNTFGQIFTVTTFGESHGKAIGCIVDGCPPNIELSELDIQPD
                     LDRRKPGTSRHVTQRREADEVEILSGVFEGKTTGTPIALLIRNTDQRSKDYGNIAESF
                     RPGHADYTYWHKYGIRDYRGGGRSSARETAARVAAGAIAKKWLHEHFGTEIIGYVTQV
                     GEKHIAFEGEEHIHNNPFFAANHSQIDELEAYMDSIRKSLDSVGAKLTISARNIPVGL
                     GEPVFDRLDADIAHAMMGINAVKGVEIGDGFDVVAQRGSYHGDELTPTGFKTNHAGGI
                     LGGISTGQDIKVNIAIKPTSSIATERESINIHGVSMTLATHGRHDPCVGLRAAPIAEA
                     MLALVLMDHALRNRAQNADVKVSTPDIEHL"
BASE COUNT          929 a         1044 c          917 g          851 t
ORIGIN      
        1 ggcctgtacc aacgcttgct gcaagttgac cctgttacag cagcgcgcct cagcgccaac
       61 gacagtcaac gcatcgaacg tgctttggag atacacgccc tcacaggaca ggcaatgagt
      121 gaattgctac aagctcaaaa cgaggccaac cacatcgcct tggaaaccgc cactttaatc
      181 ccacaacaac gcgcccaact gcaccacagc atcgccgagc gctacgatga gatgttacag
      241 gccggctttg tggacgaagt tcaggctttg caacaaaact atcctgaatt gcatgccgac
      301 ttgccttcca tgcgctgcgt gggctatcgc caagtgtggg actatttggc aggtgtgagc
      361 tcagccgatg aaatgcgaga actgggcatc atcgccacac gccaattggc caaacgccaa
      421 ttgacatggc tgcgcaaaat cgccgctact cacgcgtttg acccttacga acaccctgat
      481 ttattgccgc aagtattgcc tttaattcgc caacattttg agttttaaca tgacaaatgc
      541 tgcccaaccc gccctgctaa aacgcttctt ggctttggta tatgaaggct tgatattctt
      601 ggcagtgacc atactcgcca tcattgttgc cctgcctgtg tcttatttgt tgcgcagcca
      661 acccatgctg caacaaattg tgattacctt atggtttttc atggcctggt ggctgtatgc
      721 caaactcaat tggcgcaaag gccaaacctt ggccatgaag gcatggcatt tgcaactgcg
      781 caatgaactt ggccacattc ctgccctcca acaactgcgc atgcgttttt tgtgggcggt
      841 gttgtttttg gtattggtgc cagtattatc gtatttggtc ttaagccact ttagccaatt
      901 accacccaag ccccgctttt acctcagttt ggtgtggtgg ttactgcctt ggggctttgc
      961 ctgtgtacac ccgtccaagc aattcttgta tgatgttttg gcgggaaccc gcatcatctc
     1021 taaaaacgct gccgaacagt cctcttaagg cggatgcaca atttcgctga cttaggtaca
     1081 atttcattac ccctcaaata aggacaccat catgcgctgg caaggacgta aacaaagcga
     1141 caacatcgaa gaccgacgcg gctcaggtgg cggtggcggt tttcctggtg gccgtggtgg
     1201 catgggcata ggcggcatca tcatcgtatt agtcggctat tttgtgtttg gtgtcgattt
     1261 aacgggcatg gttggaggca acaccgcccc aagtgcccaa gtagccaacc aacctgcagg
     1321 cccagttaag caaactggtg aagaagccca actgcgtgaa ctcagcagcg tggtgttggc
     1381 atctacagaa gaatcttggg catcttattt ccaaaaacat ggcgccattt accgtgagcc
     1441 aaaaatggtg ttgtaccgcg gtgctacctc aaccgcttgt ggcacagggc aatcggccat
     1501 gggcccgttt tactgccctg ccgatgaaaa agtgtatttg gatttggcct tttatgacga
     1561 catgaaaaac caattgggtg ccgaaggtga ttttgccttt gcctatgtca tcgcacacga
     1621 agtcggccac cacgtacaaa acctgctcgg catcaaccaa aaagtcagcc aagcccaacg
     1681 cagtggcagc aaaaaacaag ccaatcaact gtcagttgcc ttagaattac aggccgattg
     1741 ttttgcgggc gtgtgggggc aatacatgca aaagcaaggc ttattggaaa tgggtgacgt
     1801 ggaaaaagcc atgaatgccg ccgccgcagt gggcgacgac cgtttgcaac agcaagccac
     1861 cggccgcatc gttcccgaca gtttcaccca cggctcttcc caacagcgca tgcaatggtt
     1921 caaccgcggt ttccaatcgg gcgaccctga acaatgcaat acctttggtt aagcggcctg
     1981 aaagcatgat atgaaaacct tacgacttta taccctcacc gtgactgccg ctttaaccct
     2041 cagtgcctgt ggctccatgg gcattggttt tggattgggc aactcaggcg gtggggtcaa
     2101 ttttggcacg ggcatctcgt ttccgctcgg cggtttgggt gtgcgcaatg accctgccaa
     2161 aagcggcatc aacctgatag atgtgcaagt ggtgagctat tttggcaccg ctgcgcaagg
     2221 ctacaaatcc agtgataatc ctatcgatgg tggcttttac cgccgcttat tgggcaagca
     2281 aggcaagctg tggctggtac aagactatta caacgattcc gaccgcaaat acaacgaacc
     2341 catgctctta agcgtggatg aggcttaccg tttcacggcc ttgccagcga gtggcacttt
     2401 ctacacctac catgagaatg gccaaatcgc cagcaagcgt gtgtatgaaa acacccgttt
     2461 gctcaaaagc gaacagtggg acaacagcgg tcacgcttta taaaccctga catgcccact
     2521 gccttacagg ccgcttacta ccccgttatt catcttcatc agaaaagaac acgcattatg
     2581 cccggcaata cttttggtca aatcttcact gttaccacgt ttggcgagag ccacggcaaa
     2641 gccataggct gcatcgttga cgggtgccca cccaatattg aattgtctga actcgatatc
     2701 caacccgatt tggacagacg caaacctggc accagtcgcc atgtcaccca acgccgcgaa
     2761 gccgatgaag tggaaatctt atcaggcgtg tttgaaggca aaaccacagg cacaccaatt
     2821 gccttgctca tacgcaatac cgaccaacgc agcaaagact atggcaacat cgcagaatct
     2881 ttccgcccag gccacgccga ttatacctat tggcacaaat acggcatacg cgactaccgt
     2941 ggtgggggtc gcagctcagc gcgtgaaacc gcagcccgtg tggccgcagg ggcaatcgcc
     3001 aaaaaatggt tgcatgaaca ctttggtact gaaatcatcg gctatgtcac ccaagtgggc
     3061 gaaaaacaca tcgcttttga aggcgaagaa cacatccata ataatccatt tttcgccgcc
     3121 aaccacagcc aaatcgacga gcttgaagcg tatatggaca gcatccgcaa gtctttggac
     3181 tcggttggcg ccaagctcac catcagtgcc cgcaacattc ccgtaggcct cggcgaaccc
     3241 gtttttgacc gcttggatgc cgacatcgcc cacgcgatga tgggcatcaa tgccgttaaa
     3301 ggcgtggaaa ttggcgacgg ctttgatgtg gtggcacagc gcggcagcta ccatggcgac
     3361 gagctcaccc ccacgggttt caaaaccaac cacgcaggcg gcatcttagg cggcatctcc
     3421 acaggccaag acatcaaggt caacatcgcc atcaagccaa cgagctcgat agccaccgag
     3481 cgcgaatcga ttaacatcca cggtgtatcc atgacgcttg ccacccacgg ccgtcacgac
     3541 ccttgcgtgg gcttgcgcgc cgcccccatc gccgaagcga tgctggcctt ggtgttgatg
     3601 gaccacgcct tgcgcaaccg cgctcaaaat gccgatgtga aggtatccac gcctgatatt
     3661 gaacatttgt aaatttcaat ccacacaaaa gcggcctgta catttacagg ccgctttttt
     3721 catgctgacg atgctgatta t
//
LOCUS       DAOMVK010000193         3733 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_7178_length_3733_cov_3.594073, whole genome shotgun sequence.
ACCESSION   DAOMVK010000193 DAOMVK010000000
VERSION     DAOMVK010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7178_length_3733_cov_3.594073"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PK856_07045"
     CDS             <1..218
                     /locus_tag="PK856_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02162.1"
                     /translation="DFFNTPRHSGSLWTSYKVTPKFTAGVGMYAQSDAVGSYTKSGDA
                     VLIKASLAMRVLMRCCLTKSAKMPKHS"
     gene            176..328
                     /locus_tag="PK856_07050"
     CDS             176..328
                     /locus_tag="PK856_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRL02163.1"
                     /translation="MLSYQISKNAKAQLNVYNLADKEYYSSTYSTHYATLGDGRAAVA
                     SLKLTF"
     gene            402..1088
                     /locus_tag="PK856_07055"
     CDS             402..1088
                     /locus_tag="PK856_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HRL02164.1"
                     /translation="MKMLVRIPQLLSVTEAQRCRELLDKQDWQDGRLTAGHIAQHVKT
                     NQQLPRDNVAAQQVGQLILEKLSESPLFMSVALPLKVLPPLFNRYEGGTTYGDHIDNA
                     VFTMPHTGEKVRSDVSTTVFLTDADAYEGGELVISDTYGEQRVKLNAGDAIVYPATSL
                     HRVEPVTAGVRVGAFFWTQSMVASGEQRQVLYDLDVAIQQVLQADAKSEAGRRLSGVY
                     HNLLRLWSHT"
     gene            1103..2239
                     /locus_tag="PK856_07060"
     CDS             1103..2239
                     /locus_tag="PK856_07060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HRL02165.1"
                     /translation="MNPAAHAHILQAALGHIPAHIQHVTDYETCAREFIADDIWHYIQ
                     SGSDQNLSLAANRRSFDQLALLPRVLRSVHEGHTRVQLFEHEHRAPLLLAPVAYHGLV
                     HPDAELATVQAAVAMQTTMVVSTLASRRFMDIRRTAEAAAQELQRPVPPLWLQLYSQP
                     TRAQTQAVLQAAAEANFDAIVWTVDAHYKRTAMALPVGVSAVNTDAEAQYKHTSQLLD
                     ECLVLGSRFTEHAPTWEDLAWLRGQTDKPILVKGIVSPADAKQALALGANGLVVSNHG
                     GRVLDGMVSPVDVLPLMRAAVGAKVPLLLDGGIRWGTDVVKAVALGADAVMVGRPQMH
                     ALAVAGMLGVAHMLHLLRVELELAMAQLGCAQVGDLNASVLHRS"
     gene            2355..2846
                     /locus_tag="PK856_07065"
     CDS             2355..2846
                     /locus_tag="PK856_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRL02166.1"
                     /translation="MAETFYQQTGSKQEQYQSLLPQIQALISDERDFVANMANVCAVL
                     KQQFDWLWIGFYVVKGEELVLAPFQGPIACTRIGKGRGVCGSAWAQQQTIVVPDVNQF
                     AGHIACSSLSQSEIVVPLLWQERCLGVLDIDSAELATFDDTDAEFLQQVVAVLLAQSD
                     LPV"
     gene            complement(2857..3321)
                     /locus_tag="PK856_07070"
     CDS             complement(2857..3321)
                     /locus_tag="PK856_07070"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRL02167.1"
                     /translation="MLIETQDANLIHAIMQQAYAEYRHDASPSSALAETVAGIEQELT
                     TGMRAFIWQPDELALAMVKTQWLPDTLYFSRLSVIPSARHQGHAQKLLTALAVQARLA
                     QLSHLECKVRAEISRNVALYQGLGFVCMAQESVHKKDGTEVPTLSMRKTLNP"
     gene            complement(3632..3716)
                     /locus_tag="PK856_07075"
     tRNA            complement(3632..3716)
                     /locus_tag="PK856_07075"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3680..3682),aa:Leu,seq:caa)
BASE COUNT          832 a          846 c         1134 g          921 t
ORIGIN      
        1 gtgacttctt caatacccca cgccacagcg gcagcttgtg gaccagctac aaagtaacgc
       61 ctaaattcac cgcaggtgtg ggcatgtacg cacaaagcga tgctgtgggc agctacacca
      121 aaagcggcga tgctgtgttg attaaggcat ccctggctat gcgcgttttg atgcgatgtt
      181 gtcttaccaa atcagcaaaa atgccaaagc acagttgaat gtgtacaact tggctgacaa
      241 agagtattac agctcgactt actctaccca ctacgccact ttgggtgatg gccgtgccgc
      301 agtggccagc ttgaaattga cgttctaatg cattaattgc gtttaagcta aaaccgcaac
      361 cccatcctcg tttaagatga tggggttgtt tctatgaaag aatgaaaatg ttggtacgca
      421 ttccccaatt gctcagtgtc actgaagcgc agcgttgtcg cgaattgttg gacaagcagg
      481 attggcaaga tggtcgcttg accgcaggcc acatcgccca acatgtgaaa accaaccaac
      541 aattgccgcg cgataacgtc gctgcccagc aagtggggca attgatattg gaaaagctgt
      601 ctgaaagccc tttattcatg tctgttgcct tgccgctgaa agtgttgcca ccgctgttta
      661 accgctatga gggcggcacc acttatggtg accatattga caatgccgtg ttcaccatgc
      721 cgcacacagg cgaaaaagtg cgcagtgatg tatctaccac tgtgtttctc acggatgccg
      781 atgcttatga gggcggtgaa ttggtcatca gcgacactta tggtgagcaa cgggtgaaat
      841 tgaatgcggg cgacgcgatt gtttaccctg ccaccagttt gcacagggtt gagcctgtga
      901 cagcaggagt gcgcgtgggg gcttttttct ggacgcaaag catggtggca tcgggtgagc
      961 aaagacaggt tttgtatgat ttggatgtgg cgatacagca ggttttacag gctgatgcca
     1021 aaagcgaagc gggtcgtcgc ttgagtgggg tgtaccacaa tttgctgcgc ctgtggtcgc
     1081 acacatgaag caggagcaga acatgaatcc agccgcacat gcgcatattt tacaggccgc
     1141 attgggccac attcctgccc acatacaaca tgtgaccgat tacgaaacgt gcgcgcgcga
     1201 gtttattgcc gatgacattt ggcactacat ccaaagcggc agcgaccaaa acctgagctt
     1261 ggctgccaat cgccgcagtt tcgaccaatt ggccttgttg ccacgcgtgc tgcgttcggt
     1321 acatgagggc catacccgtg tgcagttgtt tgagcatgag catcgcgcgc ccttattgct
     1381 ggcacctgtg gcctaccatg gcttggtgca tcctgatgct gaattggcga cggtacaagc
     1441 ggcggtggcc atgcaaacaa cgatggtggt gagcactttg gccagtcgcc gtttcatgga
     1501 catacgtcgc acggcagaag ccgcggcgca agaattacag cggcctgtac cgcccttgtg
     1561 gttacagttg tacagtcaac ctacgcgagc gcaaacccaa gcggtgttac aggccgctgc
     1621 tgaggcgaac ttcgatgcca tcgtgtggac ggtagacgcg cattacaaac gcactgccat
     1681 ggctttgcct gtgggcgtga gcgcggtgaa cacagatgca gaagcgcaat acaaacacac
     1741 cagtcagtta ttggatgagt gtttggtctt gggttcgcgc tttaccgagc atgcgcccac
     1801 ttgggaagat ttggcgtggt tgcgtgggca aaccgacaaa ccgattttgg tcaaaggcat
     1861 agtgtcgcct gccgatgcca agcaagcgct ggctttgggt gcgaatggct tggtggtgtc
     1921 caatcacggc gggcgggtgc tggatggcat ggtgtcgcca gtggatgtat tgccactgat
     1981 gcgcgctgcg gtgggggcga aagtgccttt gttgctagac ggtggcatac gttggggcac
     2041 tgacgtggtg aaagcggtgg ctttgggtgc ggatgcggtg atggtgggcc ggccgcaaat
     2101 gcatgccttg gctgtggcag ggatgttggg cgtggcgcac atgttgcact tattgcgggt
     2161 ggaattggag ctggcgatgg cgcagcttgg ttgtgcccaa gtgggcgatt tgaatgcatc
     2221 ggtattgcac cgttcttaaa cgcggttgaa acagtgaata agcggcctgt aaacggtttt
     2281 aaaaggttta caggccgctt gttttggcag gcgcaagcaa actggtatga tgggattttt
     2341 tgggcgagtg caagatggca gaaacatttt atcagcaaac aggcagcaaa caagagcaat
     2401 accaaagcct gttgccgcaa atccaagcct taatcagtga tgagcgtgat tttgtcgcca
     2461 atatggcgaa tgtgtgcgcg gtattgaagc agcaatttga ttggctgtgg attggttttt
     2521 atgtggtgaa aggtgaggaa ttggtgttgg cgccgtttca aggcccgatt gcgtgtacgc
     2581 gcataggcaa aggccgcggc gtgtgtggca gcgcgtgggc gcagcagcaa accatcgttg
     2641 tgcccgatgt gaatcaattc gcaggtcaca ttgcttgcag cagtttgtct caatcggaaa
     2701 tcgtggtgcc tttgttgtgg caagagcgct gtttgggtgt gctcgacatc gacagcgctg
     2761 aattggcgac ttttgatgac accgatgccg agtttttgca gcaagtggtg gccgtgctgt
     2821 tggcgcaatc cgatttgcca gtttgaacgg ctgtgcttac ggattcaagg ttttgcgcat
     2881 gcttaaagtc ggcacttcgg tgccgtcttt tttgtggacg ctttcttgcg ccatacagac
     2941 aaaacctagg ccttgataca aagccacatt gcggctgatt tcagcgcgca ctttgcattc
     3001 taaatggctt aattgcgcga gccgcgcttg tactgccaac gcggtgagca gcttttgggc
     3061 atggccttgg tggcgggcgg aggggatgac agacaagcga gaaaaataca gcgtatcagg
     3121 caaccattgt gttttgacca tggcgagggc cagctcatca ggctgccaaa taaatgcgcg
     3181 catgccagtg gtcaattctt gttctatgcc tgccacggtt tcggccaggg cgctggaggg
     3241 cgacgcgtcg tggcggtatt cggcataagc ctgttgcatg atggcgtgta tgagatttgc
     3301 atcttgggtt tcaatcaaca tggtggtttt gcagcaaaat aaaaagcaat atagcgcaag
     3361 aatgtgacgc ttgtgtggtt tacaggccgc attgaatgca agatggcggt gcttttgagt
     3421 gggatgcggg ttggacttgg gtggtgttga atgctgaggt gatgctgtga tttgaaggta
     3481 tgggtgtgat ttttcagcta ggggttgcgt gcttgggtaa tgtgcttgtt gattgaaaca
     3541 tggcaggtga cgcgatgaat cgattggtga tggaaatcgc acacggaaac gacaggcaaa
     3601 aaaataaccg cgaatcgcgg tttattttcc atggtgccca gggtcggact cgaaccgaca
     3661 caccttgcgg cgggggattt tgagtcccct gcgtctacca atttcgccac ctgggcagtg
     3721 gatttggctt act
//
LOCUS       DAOMVK010000194         3731 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_7185_length_3731_cov_4.482862, whole genome shotgun sequence.
ACCESSION   DAOMVK010000194 DAOMVK010000000
VERSION     DAOMVK010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7185_length_3731_cov_4.482862"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PK856_07080"
     CDS             <1..411
                     /locus_tag="PK856_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003768595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HRL02168.1"
                     /translation="KFDHCAIYLLIAGSYTPFTLVTLNGPWGWSLFGVSWGLCLFGII
                     QELTIGLRSPSRWLSMVIYVLMGWLVVVAIAPLLKNLAGWGLFWLVAGGLAYSGGIYW
                     FIKDTQIKHGHGIWHIFVLLGSLFQFICIYFYVL"
     gene            424..750
                     /locus_tag="PK856_07085"
     CDS             424..750
                     /locus_tag="PK856_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003359266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HRL02169.1"
                     /translation="MIVSTTPSLEGHKIVRYVGVVNGEAIMGANIFKDLFASVRDIVG
                     GRSGTYERELQKARDIALEELQQRAAKLGANAVVAVDLDYEVLGETNSMLMVAASGTA
                     VVIEAL"
     gene            complement(828..1667)
                     /locus_tag="PK856_07090"
     CDS             complement(828..1667)
                     /locus_tag="PK856_07090"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HRL02170.1"
                     /translation="MRHSSESVLQAGHAAHSPKPSDAQLLRSIEFHDLLKLNRTQVFV
                     NATLSWPWLILGWFLAYKTWYVPAVLATGMFYMLALRQAHDCYHKNIGLSPRNTQLLL
                     HVLAITMMCVTHAVGFTHLQHHKYKLNEHDIEGSWSRKSAWSAIVLGWVFYYKIQQNG
                     LKNAPNRLKRHAYIDLALILAVLLLTAISQQPLLIYHVASMLVCSCLVGFFAVWSVHH
                     GCDGEHDIARTERRPWLNFATAHLLFHVEHHTFPTVPCNHLPQLAKRMDEVVPHMTQK
                     RVT"
     gene            1916..2173
                     /locus_tag="PK856_07095"
     CDS             1916..2173
                     /locus_tag="PK856_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02171.1"
                     /translation="MGDWSKEPWGTDEAADWFGRYWNGGGIPLVLKTIHGFEPETEQY
                     EAVRAACMVLGCFGSAYAWPSAHLEGRLPALLQAIMLFSYA"
     gene            2421..2762
                     /locus_tag="PK856_07100"
     CDS             2421..2762
                     /locus_tag="PK856_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011133687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHCW motif cupin fold protein"
                     /protein_id="HRL02172.1"
                     /translation="MKMFPFPFTTTDWQEIIATEHPGETGMAYWRTQYFGEADQRIRV
                     RKVEYSAGYLADHWCDKGHVLFCLQGELTTRLQDGREFKLSAGMSYQVGDGVDAHQSY
                     TATGAILLIVD"
     gene            complement(2845..>3731)
                     /locus_tag="PK856_07105"
     CDS             complement(2845..>3731)
                     /locus_tag="PK856_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRL02173.1"
                     /translation="FEEAGLPAGVLNVVVGAGSEIGDYFVEHAIPRMVSFTGSTAVGK
                     RVGALAVGGRYIKRVALELGGNAPLVVLDDADLDLAVNIAAMGRFLHQGQICMSTNRV
                     IVDAKVYDAFVAKLVEKIKTVRYGNPDEMGTIVGPIINESQLSGLKKIIAQAEQDGAK
                     LVLGGEITNQVLPPHVFIDVDPQSALAKEESFGPVLPVIKARDEAHALELANDTEYGL
                     SSAVVTRNHERGVAFALQIEAGMTHINDITVDDQAHAPFGGEKNSGLGRFNGDWILEE
                     FTRTHWVTVQTQPRPYPL"
BASE COUNT          935 a          802 c          993 g         1001 t
ORIGIN      
        1 aaatttgacc actgcgccat ttacctgttg attgcgggca gttacacccc atttaccttg
       61 gtgactttga atggcccatg gggttggtct ttgtttgggg tgtcttgggg cttatgctta
      121 tttggcatca tccaagaatt gaccataggt ttgcgttcac ccagccgctg gctttcgatg
      181 gtgatttacg tgttgatggg ctggttggtg gtggtggcga ttgcgccctt gttgaaaaac
      241 ttagcgggtt ggggtttgtt ttggctggtg gcgggcggtt tggcttacag cggtggtatt
      301 tattggttta tcaaagacac acaaatcaaa catggtcacg gtatttggca cattttcgtg
      361 cttttaggca gtttgtttca gtttatctgc atttattttt acgtattgtg agaaggaatg
      421 aaaatgattg tttcgacaac accgagtttg gaaggccata aaatcgtgcg ctacgtaggc
      481 gtggttaatg gtgaggccat tatgggtgcg aatattttta aagacttgtt tgccagcgtg
      541 cgcgacattg tcggtggacg ttctggtaca tatgaacgcg aactgcaaaa agcccgtgat
      601 attgctttgg aagaattgca gcaacgtgct gccaaattgg gcgccaatgc agtggtggcg
      661 gtggatttgg attatgaggt tttgggcgaa accaacagca tgttgatggt ggccgcttcg
      721 ggtacggccg tggtcataga agccttgtaa cacattaaaa gcatgattaa gcggcctgta
      781 aactgtttac aggccgtatt tgtatgggtt tcacatgaaa cagcagctta agtcacgcgt
      841 ttttgagtca tatgtggcac cacttcatcc atgcgtttcg ccagttgcgg caagtgattg
      901 catggcacag tgggaaaagt atggtgttcc acatgaaaca gcaaatgggc agtggcaaaa
      961 ttcaaccaag ggcggcgctc ggtgcgggca atgtcgtgtt caccatcgca gccatggtgc
     1021 acgctccaca ccgcaaagaa accgaccaaa caactgcaca ccaacatgga agccacatgg
     1081 taaatcaaca agggttgttg gctgatggca gtgagcagta acaccgcaag aatcaaggcc
     1141 aaatcgatgt aagcgtggcg tttgaggcga tttggtgcat ttttaagacc attttgttgg
     1201 attttgtagt aaaacaccca gcccaataca atggcagacc atgccgattt gcgtgaccaa
     1261 gagccttcaa tatcgtgctc attcagtttg tatttgtggt gttgtaaatg ggtaaatcca
     1321 actgcatggg tcacacacat catggtgatg gccaatacat gcagcaagag ttgggtattt
     1381 cttggcgata aacctatgtt tttatggtag caatcgtggg cttggcgcaa ggcgagcatg
     1441 taaaacatgc ctgtggccaa cacagcagga acataccaag ttttgtaagc caaaaaccag
     1501 cccaaaatca gccaaggcca agacaaagtg gcgttgacga aaacctgtgt gcgattgagc
     1561 ttgagcaaat catgaaattc tatgctgcgc agcaattggg catcgctggg tttaggggaa
     1621 tgtgcagcat ggccggcctg taacacagat tcagaagagt gacgcatgat ttactccttt
     1681 ttaaatttct gtctttactg aaatgtactt aatttaaatt tcagcgtcaa tatttatttc
     1741 agtaaaaaca gaaataaata taattcattg tattcaaggt aacctttaat catgaaaacc
     1801 tgttttgaaa attggctcat aatggtttgg tgatttttag cggtgaatta ggtaggttgc
     1861 tcaatgctga gtttgagcgt gagttttgac aaagcggatt gatgcggagg ataaaatggg
     1921 cgattggagc aaagagcctt ggggcactga tgaggcagcg gattggtttg ggcgctattg
     1981 gaatggaggt ggaataccat tggtcttgaa aacgattcat gggtttgaac ctgaaaccga
     2041 gcaatacgag gctgtacgcg cagcttgtat ggtgttgggc tgctttggtt cggcatatgc
     2101 atggccgagc gcacatttgg aagggcgttt acctgcattg ctacaggcca tcatgctgtt
     2161 ctcatatgct taacccacct gatgatgcgt agggattttt agacctgtgg aatcatgacg
     2221 aagcggtggt gaaatcggta gagcagcaaa ttgctgtgtt aaatcagcat atctaagact
     2281 gtgtacgggc atggtgcagc gtaaggcgtg gttgctgggt gaagcatgta tgtttcattg
     2341 cgcatgactt tcttttcatg aggcattgct tcaccacaat gcgctatgat gtgaaaccat
     2401 atttttacag gccgcttgtg atgaagatgt tcccttttcc gtttaccacc accgattggc
     2461 aagaaatcat cgcaaccgag catcctggcg aaacaggcat ggcctattgg cgcacacagt
     2521 attttggtga agcagaccag cgcattcggg tacgcaaggt ggaatacagt gcgggctatt
     2581 tggccgacca ttggtgcgac aaaggccatg tgttgttttg tttgcaaggc gaattaacaa
     2641 cgcgcttgca agatgggcga gaattcaaat taagtgcggg catgagttat caagtgggcg
     2701 atggcgtgga tgcgcaccag tcttacactg caacaggtgc tattttactc attgttgatt
     2761 aacacacaaa cctgttgcag caagcataaa aagcggcctg taaatgttac aggccgcttg
     2821 ttcaaacatg attcatgtga aacatcacaa tgggtatgga cgtggttggg tttgcaccgt
     2881 tacccaatgg gtgcgggtaa attcttctaa aatccaatcg ccattgaagc ggcccaagcc
     2941 tgagtttttc tcgccaccaa aaggcgcatg cgcttggtca tcgacggtga tgtcgttgat
     3001 gtgggtcatg cccgcctcta tttgcaaggc aaaggccacg ccgcgctcat gattgcgcgt
     3061 gaccacggca ctggacaagc cgtattcggt gtcgttggcc aactccaaag cgtgggcttc
     3121 gtcgcgtgct ttgatcacag gcaagacagg gccaaagctt tcttcttttg ccaatgccga
     3181 ttgtggatcg acatcaatga acacatgcgg aggcaaaacc tggttggtga tttcgccacc
     3241 caataccagt tttgcgccat cttgctcggc ttgagcgatg atttttttca ggccgcttaa
     3301 ttgggattca ttgatgatgg gccccacaat ggtgcccatt tcatcaggat tgccatagcg
     3361 cacggttttg attttttcga ccagtttggc cacaaaggca tcgtaaacct tggcatcaac
     3421 tatgacgcgg ttggtagaca tgcaaatttg gccttggtgt aagaagcggc ccatggctgc
     3481 aatgttcacc gccaagtcca aatcggcatc gtccaaaacc accaaaggcg cattgccgcc
     3541 caattccaaa gccacgcgct tgatgtagcg accgcctaca gccaatgcac ccacgcgttt
     3601 gcccaccgcg gtagagcccg tgaaagacac catgcgcggg atggcatgct cgacaaagta
     3661 atcaccgatt tcgctgcctg cgcccaccac cacgttcaac acccctgctg gcaaacccgc
     3721 ttcttcaaaa a
//
LOCUS       DAOMVK010000195         3725 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_7199_length_3725_cov_3.464578, whole genome shotgun sequence.
ACCESSION   DAOMVK010000195 DAOMVK010000000
VERSION     DAOMVK010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7199_length_3725_cov_3.464578"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            266..703
                     /locus_tag="PK856_07110"
     CDS             266..703
                     /locus_tag="PK856_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02174.1"
                     /translation="MYGLILMVHLLAACVFIGTVFFEVMVLTGVKKKLPIAVSTQFEK
                     ALSQRLFAIMPWILLTLVLSGLGLLHTHYQALLALPHSSFGVLLACKLVLVLSVLLHF
                     VTIKYWRSKCMLTAQRSRLLHRSVLLHVLCIAILAKAMFYWAW"
     gene            complement(782..1984)
                     /locus_tag="PK856_07115"
     CDS             complement(782..1984)
                     /locus_tag="PK856_07115"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRL02175.1"
                     /translation="MTAFSTSSPWVCALCIMLLSACSAENEQPANTSNNAPTSASTVA
                     TASAEASKSASLAIPRIPEITIPDFIGATKQQLQFEQAMPAQLKTVAGIKVYPARCDN
                     QQLISSNGSITQYSANNATQISSKGVISINQDGSGTSTNEHLTLDVQADGSGTLVAKP
                     TSEDGEGITLTVNADGSGTYVGPEGTISVDGKGGGTWVGKLGSITINADGSGTWTGDN
                     DVVEIQANGAGNWTGTQMQTNHGDGTGTVGPEEKPVQMAPWPLAPKVGKLPLLNKLSM
                     PGQVCGYVISLDDQVLFDFDQATIRNDASTILSELSLALKQITATSIEIRGHTDSKGS
                     DEYNQTLSENRAQAVLKALQQQTQLSNISAHGYGETMPVAPNELDGKDSPSNRQLNRR
                     VEIFVKTL"
     gene            2218..2703
                     /locus_tag="PK856_07120"
     CDS             2218..2703
                     /locus_tag="PK856_07120"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HRL02176.1"
                     /translation="MEQNQDIPAIRSLIQVAFQDAPNTSHTEHLIVEALRHAHMLTIS
                     LVAEAEGRIVGHVAASPVSISSGATDWYGLGPVSVFPEKQRQGVGSRLIEAILAQLRK
                     QSAAGCVVLGEPEYYQRFGFKIEPSLVLAGVPREYFMVLSFNKDVPSGNVVYHAAFNA
                     A"
     gene            complement(2770..>3725)
                     /locus_tag="PK856_07125"
     CDS             complement(2770..>3725)
                     /locus_tag="PK856_07125"
                     /inference="COORDINATES: protein motif:HMM:NF017388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HRL02177.1"
                     /translation="YAGDFGGGLYRFNLQSPNPNMWTVTKIFQTANNQPITAAPAVFR
                     NSAEKYTVVAGTGSEIYQEDLAAKHQQALYGIFDNLTLENNSAFVNDYDLLNQTLSNE
                     VLNTSNGPLEVRKSSNNAIDETRHKGWKVNFDDSNGERITVKPSIMGNSILLSTRIYE
                     QQIIENTGDDPCLDQTKRASSSAYSWLMQFNTSNGGLIPADNKSVYIDFNLGSDARFT
                     SNANLQYMISGQKLHNLTSITLIDRLVEGFSASTSGDSGGSGEDSILNPNAKIPKNTC
                     VGEGSKAFTFDTEGQSETYNIFGACRDDAPGYKRLSWREIF"
BASE COUNT          920 a          764 c          921 g         1120 t
ORIGIN      
        1 acaactacta taacgcttca aaacctcact taatgtcgtt aaagcggcct gtaaatacct
       61 aaacgcgttt acaggccgct tattcaatga ttccaaagtt aaagtggcta taacattttg
      121 aatacaagat gcgttatcta gtttttgatg ggtaaaattt gagggattct tgtgtgtaac
      181 tgaacaggct gttgcggcat atcagtatgt attttttgct cagatttata ctgaatccca
      241 tattcatcgt tgaaatgggc atgttatgta tggtttgatt ttgatggtgc atttattggc
      301 ggcctgtgtg tttatcggca cggtattctt tgaggtgatg gttttaactg gagtgaagaa
      361 aaagctgcca attgcagtat ccactcaatt tgaaaaagcc ttgtctcaac gcttgtttgc
      421 catcatgccg tggatattac tcacattggt gctcagtggt ttgggtttac tacatacgca
      481 ttatcaggct ttattggcat tgccacacag cagtttcggg gtattgttgg cctgtaagct
      541 ggtattggta ttgagcgtgt tacttcattt tgtgaccatt aaatactggc gtagcaaatg
      601 catgctcact gcacagcgct cgcgtttgtt gcacagaagt gtgttgctgc atgtattgtg
      661 tattgcgatt ttggccaagg ccatgttcta ttgggcttgg taatgcttaa aaaagcggcc
      721 tgtaaatgtt tacaggccgc ttatgcacat gatctgggta tccactgtga aagataagaa
      781 gttatagggt tttcacaaag atttcgacac gtcgattcaa ttggcggttg cttgggctgt
      841 ctttgccatc gagctcatta ggtgcaacag gcatggtttc gccatagccg tgagctgaga
      901 tattgcttaa ctgtgtttgc tgttgtaagg ccttgagcac tgcttgggcg cggttttcag
      961 acagggtttg attgtattca tcgctgcctt tgctgtcggt atggccacga atttctatgc
     1021 ttgtggcggt gatttgcttc aatgctaggc tcaactcgct gagtatggtt gatgcatcat
     1081 tgcgtatggt ggcttggtca aaatcaaaca agacttggtc atccaaactg atgacatagc
     1141 cacacacttg cccaggcata gagagtttgt tcagtagcgg cagtttgcct actttcggag
     1201 ccaacggcca tggtgccatt tgtacaggct tttcttctgg gcctacggtg cctgtaccat
     1261 caccatgatt ggtttgcatt tgtgtgcccg tccaattgcc tgcaccattg gcttgtatct
     1321 cgaccacatc attgtcaccc gtccatgtgc cagagccatc ggcgttgatg gtaatgctgc
     1381 ccaacttgcc tacccaggtg ccacctcctt taccatcgac actgatggtg ccttcggggc
     1441 caacataagt acctgaacca tctgcattga ccgttaaggt aatgccttcg ccatcctcgc
     1501 tagtgggttt ggcaaccaaa gtacccgaac catcggcctg aacatccaaa gtgaggtgtt
     1561 cattggtaga ggtgcctgag ccatcttgat tgatgctgat caccccttta gaactgattt
     1621 gagtggcgtt gttggcgctg tattgggtga tgctgccatt gctgctgatg agttgttggt
     1681 tgtcacagcg ggcagggtaa accttgatgc ccgccacggt ttttaattga gcgggcatgg
     1741 cttgctcgaa ttgtaactgc tgtttcgtgg cacctatgaa atctgggatg gtgatttcag
     1801 ggatgcgggg tatggctaag cttgcagact tggatgcttc ggctgaagca gtagccactg
     1861 tgctggctga agtgggtgca ttgtttgaag tgttggcagg ttgttcgttt tcagcggaac
     1921 aagcgctgag taacatgatg cacagtgcac aaacccatgg acttgaagta gaaaaagcag
     1981 tcatctcttg ctctaataaa ccataacaga gcgcatcata agcaaaagcg agtcgtatct
     2041 tccaccatgt ttagatgaat taccttaaat ttacacagat tataaacctg tgctaaaact
     2101 gttctgccat ttttaggttg attgaaagag taaatcaata ataggtttca aaagtattga
     2161 gcaccgatgt agtgcgaaca gtgacaaaga gaaagcgagc atcatatgca catcagaatg
     2221 gagcaaaatc aagacattcc agccatacgc agtctcattc aagtggcatt tcaggatgcc
     2281 ccaaatacct ctcataccga gcatttaatc gttgaggcat tgcgtcatgc ccatatgttg
     2341 acaatatcgt tggtggctga ggcggaagga cgcattgttg ggcacgtggc tgcttcacct
     2401 gtgagcatca gcagtggggc aacagattgg tatggcttgg ggcctgtatc agtgtttcca
     2461 gaaaaacaaa ggcaaggcgt gggcagtcgt ttgattgagg ctatattggc acaattacgc
     2521 aaacaatctg cagcgggttg tgtggttttg ggtgagcctg agtattacca acgttttggt
     2581 tttaaaattg agccgagctt ggtgttagcg ggcgtgccta gagaatactt tatggtgctg
     2641 agttttaaca aggatgtgcc tagtggcaat gtggtttacc atgccgcatt caatgcggct
     2701 tgatatctaa aagcggcctg taaacagttt acaggccgct tgtgtaatgc ttaggctaag
     2761 attgaatact tagaagattt cacgccagct taaacgtttg tatccagggg catcatcacg
     2821 gcatgcgcca aaaatattat aggtttcgct ttggccttcg gtatcaaaag taaaggcctt
     2881 actaccctca ccgacacagg tgtttttcgg aattttggca ttggggttta agattgaatc
     2941 ctcacctgag cctcccgaat cgccactggt actggcactg aaaccctcaa ccaagcggtc
     3001 aatcaaggtg attgaagtca agttatgcag tttttgcccc gaaatcatgt attgcaaatt
     3061 ggcgtttgag gtaaagcgtg catcggaacc caagttgaaa tcgatgtaaa ccgatttgtt
     3121 gtctgcggga atcaagccac cattgctggt attgaattgc attaaccaac tgtaggcgct
     3181 gcttgaggct cgcttggttt gatcaagaca tggatcgtca cccgtgtttt caatgatttg
     3241 ctgttcataa atacgggtag acaggagaat ggaattgccc atgatgcttg gtttaacagt
     3301 gatgcgttcg ccgttactgt catcaaaatt gaccttccac cctttatgtc tggtttcatc
     3361 aatggcattg ttactggatt tacgaacctc tagtggacca ttacttgtgt taagaacttc
     3421 attggacaaa gtttggttta ataaatcata gtcgttaaca aaagctgaat tgttttccaa
     3481 tgtcaaatta tcgaaaatgc catataaggc ttgttggtgt ttggcagcca agtcttcttg
     3541 ataaatttca ctcccagttc ctgccacaac agtatatttt tcagcactat tacggaacac
     3601 tgcgggggct gcagtgatgg gctgattgtt ggcggtttgg aaaattttgg taaccgtcca
     3661 catattggga ttagggcttt gtaaattaaa gcgatacaaa cctccaccaa aatcacccgc
     3721 gtaag
//
LOCUS       DAOMVK010000196         3719 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_7224_length_3719_cov_3.694596, whole genome shotgun sequence.
ACCESSION   DAOMVK010000196 DAOMVK010000000
VERSION     DAOMVK010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7224_length_3719_cov_3.694596"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="PK856_07130"
     CDS             complement(<1..140)
                     /locus_tag="PK856_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRL02178.1"
                     /translation="MQPTIESLGLVPMVVEQSGRGERAYDIYSRLLKERIIFMVGPVT
                     DE"
     gene            complement(222..1535)
                     /gene="tig"
                     /locus_tag="PK856_07135"
     CDS             complement(222..1535)
                     /gene="tig"
                     /locus_tag="PK856_07135"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRL02179.1"
                     /translation="MDVTVENLEGLNRKITLALPWEGIRAAVDKKLSQTQRKAKVQGF
                     RPGKAPLKMVDAMYGADIRNEVLNDAVVKAFYEVVEAQKLRVAGLPRFNEVENQDDEN
                     TFKIDAQFEVFPEVKVGDLSAQEVEKATTEVSDAEVDKTIEILRGQRTRYNHVERAAQ
                     KEDRVIIDFAGKIDGEAFDGGSAENYPFVLGQGQMLPEFEAGVEGLKEGESKDVEVSF
                     PEDYHGKEVAGKTAVFTITLRNVSAATLPEVDEEFAKQLGIVDGDVAKMRDEVKKNVS
                     REVNRRVAEKNKAAVMDALLAVTEFDVPAALVQEETGRMMQDARQNFINQGFDAKQLP
                     ELPADMFTEQATRRVKLGLILAQLVEEQQLQPTNDDIRDIVAEFAESYEDPAEVIEWY
                     MGDAERQQGPAALATEAKVVEFVLGKAKVTDKALSFDEVMGNTAA"
     gene            complement(1700..2560)
                     /locus_tag="PK856_07140"
     CDS             complement(1700..2560)
                     /locus_tag="PK856_07140"
                     /inference="COORDINATES: protein motif:HMM:NF020481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HRL02180.1"
                     /translation="MNYSLDALWWELQTDAVRDLASLLTAPSLWQTDCELSRRELLGD
                     TGFRYLLALDNKPDVLYQYLQAASRQSHRLGFYAEHLLAFWFAHAPHCQLLGQNIVLQ
                     EAAHTPTIGALDYVARINDRLYHLELTCKYYAHSSADPHGLRGIQAKDTLASKAAKLM
                     QQLALHEHPVFAAWCEAQQLQQADLQAASVVRGMGFWLGDVVQSPINPYAWRGEYLEN
                     QAAWPHIEGCRYAAIPSKRLLSPLRLPFEATLAHGQVGSENGVWWAQLQQRPDGHWHE
                     ISRSMCLQNA"
     gene            complement(2576..3382)
                     /locus_tag="PK856_07145"
     CDS             complement(2576..3382)
                     /locus_tag="PK856_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRL02181.1"
                     /translation="MEKPQLPDFLQNITVVLARTSHPANIGSAARAMKTMGLTQLVLV
                     APNLIATPMTPVVPEFNPEAVSDFSLPEESFVLASGAKDVLENARIVATLQEALADTT
                     MSCALTSRKRELTATMHTPRELCPSLIDAARRGEKVALVFGNETFGLSIEEVMACNRL
                     MTINGNPNYFSLNLAQAVQVVCYELFSHVDVSMDYLRPEHEYATHEQVTGMVGHLQAA
                     MDSVGFFDRRPPERLMRRMQAVFDRAQLEREEIDILRGFFHNIEKQVKKD"
     gene            3536..>3719
                     /locus_tag="PK856_07150"
     CDS             3536..>3719
                     /locus_tag="PK856_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRL02182.1"
                     /translation="MTPFLNTALKAARRAASMMTRASNNLDSVVYDSKAFNDFVSEVD
                     KESERIILEVLSEAYPH"
BASE COUNT          901 a         1006 c          839 g          973 t
ORIGIN      
        1 ctttcatcgg tgacagggcc taccatgaaa ataatgcgtt cttttaacaa gcgcgaataa
       61 atatcgtaag cgcgttcgcc acgaccgctt tgttccacca ccataggcac taagcccaaa
      121 gactcaatag tcggttgcat caggtctcac ctcttgtcac gattgtcaaa aaaagcacca
      181 aaacaacacc tgctgttttg gtgctcatct gtgtattaaa cttaagcagc agtattgccc
      241 attacttcgt caaaagacaa ggctttgtcg gtcactttgg ctttacccaa aacgaattct
      301 acaactttgg cttcggtagc caatgctgct gggccttgtt gacgttcagc atcacccata
      361 taccattcaa tcacttccgc tggatcttcg tagctttcag caaattcagc cacgatgtca
      421 cggatgtcgt cgttggtagg ttgcaactgt tgttcttcta ccaattgagc caaaatcaaa
      481 cccaatttca cgcggcgagt cgcttgctca gtgaacatgt cagcaggcaa ttctggcaat
      541 tgtttggcat cgaaaccttg gttgatgaag ttttgacgcg catcttgcat catgcggcca
      601 gtttcttctt gtaccaaagc agcaggcaca tcaaactcgg tcacagccaa caaagcatcc
      661 attaccgcgg ctttgttttt ctcagccaca cggcgattta cttcgcgaga cacgtttttc
      721 ttcacttcgt cgcgcatttt ggcaacgtcg ccatccacaa tgcccaattg tttggcaaat
      781 tcttcgtcca cttctggcaa agtcgccgca gacacgttac gcaaagtgat ggtgaacacg
      841 gcagttttac cagccacttc tttaccatgg tagtcttcag ggaagcttac ttccacgtct
      901 ttgctttcgc cttctttcaa gccttcaaca ccggcttcga attctggcaa catttggcct
      961 tgacccaata cgaatgggta gttttcagca gagccaccat caaacgcttc gccgtcgatt
     1021 tttccagcga aatcaatgat cacgcggtct tctttttgcg cagcacgttc aacgtggttg
     1081 taacgggtgc gttgaccacg caaaatttcg atggttttgt ctacttcagc atcgctcact
     1141 tcggtagtgg ctttttcaac ttcttgagca gacaaatcgc ctactttcac ttctgggaac
     1201 acttcaaatt gggcatcaat tttgaaggtg ttttcgtcgt cttggttttc cacttcgttg
     1261 aaacgtggca agccagcaac gcgcaatttt tgagcttcaa ccacttcgta gaaagctttt
     1321 accacagcgt cgttcaacac ttcgttgcgg atgtccgcac cgtacatggc gtcaaccatt
     1381 ttcaaaggcg ctttacctgg gcggaagcct tgtacttttg ctttgcgttg ggtttgtgac
     1441 aattttttgt ctacggcggc gcggatgcct tcccatggca aagccaaagt gattttacgg
     1501 ttcaagcctt ccaagttttc tactgttacg tccataccca ttaaccttat ctgttatcta
     1561 aattaattta accacgcccg catcggttta caggacgcat atcaaagctc gcccagcccg
     1621 tatttcagtc aaaacaccaa aaaacagcta ggacaaatag tccgctagtt tagcataatc
     1681 aagtcaaatt gaaacccgtt tacgcgtttt gcaagcacat agaacggctg atttcatgcc
     1741 aatgtccatc tggcctttgt tgtaactgcg cccaccacac gccgttttcg ctgcccactt
     1801 gcccgtgagc cagtgtggcc tcaaatggca aacgcaaggg cgagagcaaa cgcttggatg
     1861 gtatcgccgc gtagcgacag ccttctatgt gtggccacgc ggcttggttt tcaagatact
     1921 caccgcgcca tgcatacgga ttaatcggtg attgcaccac atcgcccaac caaaacccca
     1981 tgccacgcac cacagaagcg gcctgtaaat cggcttgttg tagctgctgt gcctcacacc
     2041 aagcggcaaa cacaggatgc tcgtgcaagg ccaattgctg catgagcttg gctgccttgc
     2101 tcgccaaagt gtctttggcc tgtatgccac gcaagccatg tgggtcggca ctgctgtgag
     2161 cgtaatactt acaggttaat tccaagtggt agagcctgtc gttgatgcgt gccacataat
     2221 ccaaagcgcc gatggtgggc gtgtgcgctg cctcttgcag cacaatattt tgccccaaca
     2281 attggcaatg tggcgcatgt gcaaaccaaa acgccaacaa atgctcggca taaaacccca
     2341 aacggtgcga ttgccgagaa gcggcctgta aatactgata caacacatcg ggcttattat
     2401 ccaaggccag caaatagcga aagcctgtgt cgcccaaaag ctcacgccgt gacaactcac
     2461 aatcggtttg ccacaaagat ggcgcagtga gcaagctggc caaatcgcgc acagcgtcgg
     2521 tttgcaattc ccaccacaaa gcatccaagg aataattcat ttacaggccg ctttctcagt
     2581 cttttttgac ctgtttttcg atgttgtgga agaaaccacg caaaatgtcg atttcctcac
     2641 gctccaactg ggcgcgatca aacaccgctt gcatgcggcg catcaaacgt tcaggggggc
     2701 ggcggtcgaa aaagcccacg ctgtccatag cagcctgtaa atgccctacc atgcctgtga
     2761 cttgctcatg cgtggcgtat tcgtgttcgg ggcgcagata atccatgctc acgtccacat
     2821 ggctgaacaa ttcataacac accacttgca ccgcttgcgc caaattcaac gagaagtaat
     2881 tcgggttgcc attgatggtc atcaagcggt tgcacgccat cacttcttcg atgctcaaac
     2941 caaaagtttc attaccaaac accagtgcca ctttttcgcc acggcgtgcc gcatcgatca
     3001 agcttgggca caattcgcgc ggtgtgtgca tggtggcggt gagctcgcgt ttgcggctgg
     3061 ttaaggcgca gctcatggtg gtgtcggcca aagcctcttg caaagtcgcc acgatgcgcg
     3121 cgttttccaa cacatctttc gcgcccgaag ccaacacaaa gctttcttct ggcaaggaaa
     3181 aatcgctcac ggcctctgga ttaaactctg gcacaacagg tgtcattggg gtggcaatca
     3241 gattgggcgc caccaacacc aattgggtca aacccatggt tttcatggcc cgtgcggccg
     3301 agcctatgtt ggcaggatgc gaagtacgcg ccagcaccac ggtaatattc tgtaaaaaat
     3361 caggtaattg gggtttttcc atgtcgtttg cttcttggga tagtgcgcga attcgtttat
     3421 aatacgcgcc ttagatttat ttcgatcttt aacagctttg tctttgccgc ctcgtcgcac
     3481 ctgcgacgaa agaggctatt gtaacgctac acccgatgaa tttggaaatc acaacatgac
     3541 cccatttttg aatacggcct tgaaagccgc tcgccgcgct gccagcatga tgacccgcgc
     3601 ttctaataac ttagattctg tggtttacga cagcaaggcg tttaacgatt ttgtgtctga
     3661 agtcgataaa gaatcagaac gcatcatttt agaagtgttg agcgaggcct accctcacc
//
LOCUS       DAOMVK010000197         3710 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_7245_length_3710_cov_3.222709, whole genome shotgun sequence.
ACCESSION   DAOMVK010000197 DAOMVK010000000
VERSION     DAOMVK010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7245_length_3710_cov_3.222709"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /gene="trmD"
                     /locus_tag="PK856_07155"
     CDS             <1..512
                     /gene="trmD"
                     /locus_tag="PK856_07155"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009019 - tRNA
                     (guanine-N1-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRL02183.1"
                     /translation="HQGKNAPRVLYLSPQGQPLTHAKTQELAQLDGLILLCGRYEGVD
                     ERLLERHIDEEIAVGDFVVSGGELPAMMLMDSVLRQIDGVLGHQDSAAQDSFANGLLD
                     CPHYTRPVSFENMHVPEVLLSGNHAKIAKWREQQSVLRTTQRRPDLLAALKQEASELG
                     NSEPTDKAI"
     gene            528..899
                     /gene="rplS"
                     /locus_tag="PK856_07160"
     CDS             528..899
                     /gene="rplS"
                     /locus_tag="PK856_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRL02184.1"
                     /translation="MNIIEQLEQEEIARLNKEIPAFAPGDTVIVSVSVTEGTRTRLQA
                     YEGVVIARRNRGLNSNFIVRKISNGEGVERTFQLYAPSVAKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKVAK"
     gene            989..1315
                     /locus_tag="PK856_07165"
     CDS             989..1315
                     /locus_tag="PK856_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02185.1"
                     /translation="MKKVYLGVLAGMLISSPVFADWLPSLPSPDKWLNQQCMSRIAQY
                     PKVKLNSSERRNTCACMTQHAKANLSTRDILDLAQMSDSELERKYGSQAKQSFERCSK
                     QQMASQ"
     gene            complement(1410..2345)
                     /gene="ppk2"
                     /locus_tag="PK856_07170"
                     /pseudo
     CDS             complement(1410..2345)
                     /gene="ppk2"
                     /locus_tag="PK856_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003824635.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
     gene            complement(2447..>3710)
                     /locus_tag="PK856_07175"
     CDS             complement(2447..>3710)
                     /locus_tag="PK856_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003822834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HRL02186.1"
                     /translation="PELFLRIQADGHILTKPMKGTAPILGDGQDAERAVFLQNDAKNR
                     AENVMIVDLLRNDLGKLAVAGGVSVPQAFEVTAFGSVWQMTSTVVAQMRPETTVANII
                     AAAFPCGSITGAPKRMAMQVIGELEQRDRGMYTGSVGYLEPCATGLGFQGTWNVVIRS
                     LALTEQATPQRYHVSMGIGSGIVIDSRGADEWDECAWKARFVRGLPAEVGLIETLRVE
                     NGVCEWLVLHQARLQQSAADLHITIDESRLWQDLQAACASEWAEGVWRVKCSIDADGS
                     HDWQAAPLAALEGAQLVCFSETILPKHDVLRRYKTQAREQFDVVWQQAVNQGAFDGLL
                     FNEEGVLLEGGRSNVFVEIDGAWYTPALDLDVLNGVMRQAVMAKPSRFGFVGSIQESR
                     SITREDLQAATQIRLSNALRGVFEVSLM"
BASE COUNT          948 a         1037 c          854 g          871 t
ORIGIN      
        1 aacaccaagg taagaacgcg ccacgtgtgc tgtatttaag cccacaagga caacctctta
       61 cccatgccaa aacgcaagaa ttggcacaac tggatggctt gattttactg tgtggccgct
      121 atgaaggcgt ggatgaacgc ttgctggagc gccacataga tgaagaaatt gctgttgggg
      181 attttgtggt atctggcggg gaactgccag cgatgatgtt gatggattcg gtattgcgtc
      241 aaattgacgg ggtattgggc caccaagatt cagccgcaca agactcattt gccaacggtt
      301 tgctcgattg tccgcattac acgcggcctg taagctttga aaacatgcat gttcctgaag
      361 tattgttgtc aggaaaccat gccaaaattg caaaatggcg agaacaacag tctgttttgc
      421 gtaccacgca acgccgacca gatttactgg cagcgttgaa acaagaggcg tctgaactcg
      481 gaaattccga accaacagac aaggccatat gatatatagg aaatgtaatg aacattattg
      541 aacaattaga gcaagaagaa attgctcgtt tgaacaaaga aatcccagcg tttgcaccag
      601 gcgacaccgt aatcgtgtct gtgtctgtga ctgagggtac gcgtacccgt ttgcaagctt
      661 acgaaggtgt ggtaattgca cgtcgtaacc gtggtttgaa ttctaacttc atcgttcgta
      721 aaatctctaa cggtgaaggt gttgaacgta cattccaatt gtacgctcca tcagtagcga
      781 aaatcgaagt aaaacgccgt ggtgacgttc gccgcgcgaa attgtactac ttacgtggtt
      841 tgactggtaa agcggctcgc attaaagaaa aattgccagc acgtaaagtg gccaaataag
      901 aagtatttgc ttaaaaatac gttaaaaacg gtgtcttatg gcaccgtttt ttctatgatg
      961 tagaccgata ctcgaatgtg ggaccgtcat gaaaaaagtt tacctgggtg ttttggcagg
     1021 catgttgatc agcagccccg tcttcgcaga ttggttgccc agcctcccgt cgccagacaa
     1081 atggctgaac caacaatgca tgagccgcat cgcccaatac cccaaagtca aactcaatag
     1141 cagcgaacgc cgcaatacct gtgcttgcat gacccaacac gccaaagcca atttatccac
     1201 ccgcgacatc ttggatcttg cgcaaatgtc cgacagtgaa ttggaacgca aatacggtag
     1261 ccaagccaag cagtcttttg agcgttgcag caaacagcaa atggccagtc aataagcctt
     1321 attgacctga cacacatcaa aagcggcctg taaaccttac aggccgcttt ttctatgcat
     1381 gcattgctga tgcaagcatg atactggatt caatcgtgca cactgtccaa gctgcccgat
     1441 ttgctgtggg cattgggcac caacacgatt ttgtcatcgg taggccgtat ggctttgagg
     1501 tctttgccag ggtaatccaa gctgtgcaag aaaaaacgga tgcaattgag gcgcgcacgc
     1561 tttttgtcgt cagactaaat caccgtccac ggcgcatcgc ctgtgtgggt gtggaaaaac
     1621 atttggtttt tggcctcggt atactcgtcc cagcgatcta aagactgtat gtcgattggc
     1681 gagagcttcc aatgttttaa cgggtcatcg cggcgcgaaa tgaaacggcg caactgctct
     1741 tcacggctca ctgaaaacca aaacttgaac aagtggatgc catcattgac cagcatgcgc
     1801 tcaagctctg gcgtttggcg catgaattgc aaatactctt gcggctcgca aaaacccatc
     1861 acccgctcga cacctgcgcg gttataccaa gagcggtcaa acaacaccat ttcacccgcg
     1921 gttggcaaat gttccacata gcgctgaaaa taccattggc ctttttctac ctcgttgggt
     1981 ttttccaaag ccaccacgcg cgcgccacgc ggattcaaat gctccataaa acgcttaatc
     2041 gtgccaccct tgcctgccgc atcgcgccct tcaaacaaac acacaatgcg ctgccccgtt
     2101 tccttcaccc acgattgcac tttcagcaac tcgatttgca gcaattgctt atcatgctca
     2161 tacacacggc ggctcatgcg ggttttatac gggtaagaca aaggcaacgg cgccgtttgt
     2221 gcgtcttcag cactgttgcg tccctctgct tccaaaacct tttgggtaaa aatcggcaag
     2281 gcttccgcct cagcctccga tgctaatgct tcttcggcat gctcctgagt ggattgtgga
     2341 ttcaaaattt catctgccat gtttcaacct ttcatcaaga aaataccgtc tacacattat
     2401 aaatcgtgcc ttgaccaaac tcatgtttat gcttcaagca cttgcgctac atcaagctta
     2461 cctcaaacac accgcgcaaa gcattgctca aacgtatctg agtagcggcc tgtaagtctt
     2521 ctcgggtaat gctgcgtgat tcttgtatgc tgccgacaaa gccaaagcgc gatggcttag
     2581 ccatcaccgc ttggcgcatc accccattta acacatccaa atccaaagca ggcgtgtacc
     2641 aagccccatc gatttccaca aacacattgg agcggccacc ttcgagcaac acgccctcct
     2701 cattgaacaa caagccatcg aatgcacctt gattcactgc ttgctgccac accacatcaa
     2761 actgctcgcg cgcttgggtt ttgtagcggc gtaacacatc atgcttgggc aatatggttt
     2821 cagagaaaca cactaactgc gcgccctcta gtgccgccaa aggtgcggct tgccagtcat
     2881 gactgccatc ggcatctatg ctgcatttca cccgccacac accctcagcc cactcgctcg
     2941 cacaagcggc ctgtaaatct tgccacaagc gactttcatc gatggtaatg tgcaaatctg
     3001 ccgcactttg ctgcaaacgc gcttggtgca aaaccaacca ttcgcacacg ccgttttcca
     3061 cccgcaaggt ttcaatcaag cccacttcgg caggcaggcc gcgcacaaag cgcgctttcc
     3121 aagcacactc gtcccattcg tctgcgccac ggctgtcgat gacaatgccc gagcctatgc
     3181 ccatgctcac atggtagcgc tgcggcgtgg cttgctcggt caaggccaac gagcggatga
     3241 ccacattcca agtgccttga aaacccaagc ctgtggcaca cggctccaaa tagcccacgc
     3301 tgcctgtgta catgccgcgg tcgcgctgct ccaattcgcc tatcacttgc atcgccatgc
     3361 gcttgggcgc gcctgtgatg gaaccacaag gaaaggccgc ggcaatgata ttcgctaccg
     3421 tggtttcagg ccgcatttgc gccaccacgg tagacgtcat ttgccacacc gagccaaacg
     3481 cggtcacttc aaacgcctgt ggcacactca cgccaccggc caccgccaac ttgcccaaat
     3541 cattgcgcag caagtctact atcatcacat tctcagcgcg gtttttggca tcgttttgca
     3601 aaaacaccgc gcgttcagca tcttggccat cgcccaaaat cggcgccgtg cctttcatcg
     3661 gcttggtgag aatgtgacca tcggcctgta tgcgcaaaaa cagctcaggc
//
LOCUS       DAOMVK010000198         3698 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_7275_length_3698_cov_2.129564, whole genome shotgun sequence.
ACCESSION   DAOMVK010000198 DAOMVK010000000
VERSION     DAOMVK010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7275_length_3698_cov_2.129564"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2928
                     /locus_tag="PK856_07180"
     CDS             <1..2928
                     /locus_tag="PK856_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HRL02187.1"
                     /translation="NADGVLQKEQAQEVLAADVRLPNSHDEGYTTAEAYRQALLAQGV
                     SKQDAQIRVSEYKIRLELAKLKIIAGVLGVPLGELTKRDQAFQLQKAQHKNRIFKRVA
                     TAIGVLALAAIMAGGLAWVKKTEAQKNLSLSLYNSGINKLTESEYGDGAAFIAAAARS
                     GDANAALFGQSMLALQENLTLMPNLTPGASQFSPDGNWLVGYANNGQGNLKLQIWDVN
                     RKRLLKQIDSISTDQPKTPLFDTKNNAYLQTKDQELLQLNIQNFATKPVFKAQADELI
                     RVLEVSPNGQWLVIRSVGSTELKHQQYYLVDLHTQNRYPITTYDAIASLTATAFTPNS
                     THLLFNIKKENQTNDVKVFDLSKPQTAIFSTTSPNEYIDASINPNGDAVLINTNKNID
                     FFDLKSGHHWQKPKPVGGYSFVSFNPDGEHLVAANDNKLLLLDEQNGEVTAETELRSL
                     GALNSMSQVAKQPEEAKKSLSAKLTHDMTQELVVLNKQPFLRTLSNKPQLKFERMMVA
                     ELKILRPAVDQKQVFVVKEDSKNLYRLPVNNQQDMQLFASFPQDITNVQVLKKTPYLL
                     VRTGKVIQVLDNQTGKPVGKPITLPSEKKVYLMDASDQKILARSSDHKLTIWEVATGN
                     EVGHYEQAQPIAPFITDGQFKYMLQAGDKDYTVTELATGKIIKQESKPLTGAKFSPDA
                     RYLEFTERGGKGTVMSMTELKPLFNFNSIENPFIAFSHDSRVMAVSEDARYMRLWDLT
                     ASKAFGQRIKISQKNPVFEFNVDNTRIFVQSDDNKYGSETLLIDAKTGIPLTMPFAQG
                     QYDDSFMLGDTDVLTLAQTGSTFTIRDWEVPGQLNMESTQVADDLEQYYGKVLDLNTG
                     AIDAYVKKDQAFNTWYFQDAYTRTITPASSTSILDVIKPHMSFKQPEDEVILSETFTY
                     HPLARAALAEHFSQKIETAYLGQRYLEITQAQLPKVHNAMLKQQVETLLAQAQKNLQG
                     LQP"
     gene            2925..>3698
                     /locus_tag="PK856_07185"
     CDS             2925..>3698
                     /locus_tag="PK856_07185"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HRL02188.1"
                     /translation="MNLSRKSKGLIMLALFAAVAVLSWIGLSRLYPESNTLRYLPDRI
                     YRIIKILMGTDPTSSGLEKPDTPWQLIIVKLFSIYILALLVFKGLQKFFHEQWVLYRI
                     AFMKNHNIVVGMGEKSLRLLKDMSEQGMKPVVIEQNEEHPHKKQVRKWGHATVFGDAS
                     DDKVLEEAACKHAQSLVCFLDDEQKVLNVAKAVKIHHQDATQEQAMLAHLHFRHPRIV
                     ELLQQSALCQNHDHQVQWRFFNIQKMVARQFMQQLAHDLS"
BASE COUNT         1157 a          853 c          814 g          874 t
ORIGIN      
        1 aatgccgacg gggtgctaca aaaagaacag gcacaagagg tgttggctgc ggatgtgcgc
       61 ctgccaaaca gccatgatga gggctacacc accgcagagg cttatcgaca ggctttgtta
      121 gcacaaggcg tcagcaagca agacgcccaa atacgggtaa gcgaatacaa aatccgtttg
      181 gaattggcga agctcaaaat catcgctggt gttttgggcg tgcctttggg agaactcacc
      241 aaacgcgacc aagcgtttca attgcaaaaa gcccaacaca aaaaccgcat cttcaaacgc
      301 gtggccacgg ccataggtgt gttggcattg gccgccatca tggcaggagg attggcttgg
      361 gtaaaaaaaa ccgaagcaca aaaaaacctg tctttgtctt tgtacaattc tggcatcaat
      421 aaactgaccg aaagtgaata tggcgatggc gctgcattca ttgcggctgc agcccgcagt
      481 ggcgatgcca atgccgcttt gtttggtcaa tccatgctgg cactgcaaga aaatctgaca
      541 ctaatgccga atctcacacc tggagcttca cagttcagcc cagatggcaa ctggcttgta
      601 ggctatgcaa acaacggtca gggtaactta aaactacaaa tctgggatgt gaatcgcaag
      661 cgcttgctca agcaaattga cagcattagc actgatcaac ctaaaacacc tctttttgat
      721 accaaaaaca atgcttattt acagaccaaa gaccaagaat tgctgcaact gaacatccaa
      781 aatttcgcaa ccaagccggt gttcaaagcc caagcagacg aattgattcg ggtacttgag
      841 gtcagcccaa atgggcaatg gttggtcata cggtcagtag gcagcaccga attgaaacac
      901 caacaatact atttggtaga tttacacact caaaatcgct atccaatcac cacgtatgat
      961 gccattgcca gtttaacggc aacagcgttc acacccaact ctacccattt gctttttaat
     1021 atcaaaaaag aaaatcaaac caatgatgtg aaagtttttg acctctcaaa accgcaaaca
     1081 gcaatatttt ccaccacaag tcccaatgaa tacattgatg cgagcatcaa tcccaatggt
     1141 gatgcagttt tgattaacac caataaaaac attgattttt ttgaccttaa atcgggtcac
     1201 cattggcaaa agcctaaacc tgtgggcggc tacagcttcg tatccttcaa tccagacgga
     1261 gagcatttgg ttgcagccaa tgacaataaa ttactgctgc ttgatgaaca aaatggcgaa
     1321 gtcaccgcag aaaccgagtt gcgcagcttg ggtgcgttga actcgatgag ccaggtggcc
     1381 aaacaacccg aagaagccaa aaaatcttta tctgcaaaac tcacgcacga catgactcaa
     1441 gaactggtgg ttctaaataa acagcctttt ctcaggactc tgagtaacaa accacagttg
     1501 aagtttgaac ggatgatggt cgccgagctg aagatcctgc gcccagccgt tgatcaaaaa
     1561 caggtatttg tagttaaaga agacagtaaa aatctttatc gtctgccagt aaacaatcaa
     1621 caagacatgc aattgtttgc gagctttccg caagacatca ccaatgtaca agtgttgaaa
     1681 aaaacacctt atcttttggt gcgcacaggc aaggtcatcc aagttttgga caatcaaacg
     1741 gggaaacccg tagggaaacc gattaccctg ccctcagaaa aaaaagttta tttgatggat
     1801 gccagtgatc aaaaaatatt ggcacgcagc tctgaccata aactcaccat ttgggaagtc
     1861 gctactggaa atgaagtagg ccattatgag caagcccaac ccatagcgcc ttttatcact
     1921 gacggtcaat ttaagtatat gttacaggca ggagataaag actataccgt gacagaattg
     1981 gccacgggta aaatcattaa acaagagtct aagccattga ctggcgcaaa attcagtcct
     2041 gacgcacgtt acttagaatt tactgagcga ggtggaaaag gcacggtcat gagcatgact
     2101 gagctgaaac ccttatttaa ctttaacagt attgagaacc catttattgc attcagccat
     2161 gacagccgag tgatggcagt gtctgaagat gcaagataca tgcgcttatg ggatttaact
     2221 gcctccaagg catttggaca gcgcatcaaa atcagccaaa aaaatcctgt ctttgaattc
     2281 aatgtcgaca atacacgcat ctttgtgcaa agcgatgaca ataaatatgg cagtgaaacc
     2341 ttgctcattg atgccaaaac gggcatccct ttgacaatgc cgtttgctca aggccagtat
     2401 gatgacagct ttatgctggg agacaccgat gttttaacct tggcgcaaac tggcagcacg
     2461 ttcaccatcc gtgactggga agttccgggt cagctcaaca tggaatcgac gcaagtggcc
     2521 gatgacttgg aacagtatta tggcaaagta ttggatttga acacgggcgc catcgatgcc
     2581 tatgtgaaaa aagaccaagc gtttaataca tggtattttc aggacgctta tacccgtacg
     2641 attacccctg cctcaagcac ttccatactg gatgtcatca aaccacacat gtcattcaaa
     2701 caacctgaag atgaagtgat tttgagtgaa acatttacct accatccatt ggcacgtgca
     2761 gccttggcag agcatttcag tcagaagatt gaaacagctt atttgggaca acgctatctg
     2821 gaaattaccc aagcccaatt acccaaagta cacaacgcca tgttaaaaca acaagtggag
     2881 actttattgg cccaagctca gaaaaattta caagggttgc agccatgaat ttaagccgta
     2941 aatccaaagg cctgatcatg ctggcgttgt ttgctgcggt ggcggtattg tcgtggattg
     3001 gtttgtcacg cttgtatcca gaaagcaata ccttgcgata tttgcctgac cgcatttacc
     3061 gcatcatcaa aattttgatg ggcactgatc caacctccag tggtctggaa aaaccagaca
     3121 cgccatggca actgatcatc gtcaaactgt tttccattta catcttggcc ttgttggtat
     3181 tcaaaggctt gcaaaagttt tttcatgagc aatgggtgtt gtaccgcata gcattcatga
     3241 aaaaccacaa cattgttgtc ggcatgggtg aaaaaagcct gcgcttgctc aaagacatgt
     3301 cagagcaagg catgaaaccg gtggttattg agcaaaatga agaacacccc cacaaaaaac
     3361 aagtgcgtaa atgggggcat gccacggtgt ttggtgacgc cagtgatgac aaggtattgg
     3421 aagaagcggc ctgtaaacat gcgcagagct tggtgtgttt cttagacgat gagcaaaaag
     3481 tactcaacgt ggccaaagca gtcaaaatac accaccaaga tgcaacacaa gagcaagcca
     3541 tgttggcaca tttgcacttt cgccacccgc gtatagtcga gttgttacaa caatctgcct
     3601 tgtgccaaaa ccacgaccat caagtgcaat ggcgcttttt taacattcaa aaaatggttg
     3661 caagacaatt catgcagcaa ttggcgcatg atttatcc
//
LOCUS       DAOMVK010000199         3697 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_7280_length_3697_cov_2.415980, whole genome shotgun sequence.
ACCESSION   DAOMVK010000199 DAOMVK010000000
VERSION     DAOMVK010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7280_length_3697_cov_2.415980"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..799
                     /locus_tag="PK856_07190"
     CDS             62..799
                     /locus_tag="PK856_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRL02189.1"
                     /translation="MTSVSNPIALVTGGSRGIGAEIAQRLAQDGFTVAVNYTSNPAAA
                     DEVVAQITADGGKAFAVQADISDAAAVAAMFDDIAKRGDLTVLVNNAGIMNLAPIAEM
                     DSGVFERMLNINLLGSFNTMQQAAKHMVAGGRVINTSSSVTKLLQPTYGPYAATKAAV
                     EAMSVVFARELRGRSITVNTIAPGPTASALFLNGKTPEQIQHLENISPLQRLGQPSDI
                     AAVVSFLAGPDGAWVNGQTLFANGGAI"
     gene            complement(1018..2229)
                     /gene="ubiM"
                     /locus_tag="PK856_07195"
     CDS             complement(1018..2229)
                     /gene="ubiM"
                     /locus_tag="PK856_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HRL02190.1"
                     /translation="MKKGSSMQTDIVVVGAGPAGLSLVRALAGSGLSVALVERNDYSS
                     IAHPTYDGREIALTHASQALMQQYGLWQHLPENEIYPLRDAKVVSGASDFTLHFPQPK
                     PEAGLIAEQLGFLVSNHNIRQAAFNAAEPVENVAWLTGVNVEQVQVQVADDEATVKLS
                     DGRSLHTRLVVAADSRFSNTRRQLGISADSHDYGRTVLVFRTEHEISNQATAFECFHY
                     GRTLALLPLSSHMTNCVVTANHQIAKRLLALSPEALAQDMAQQLQGRLGKMQVISSVH
                     QYPLVGVHARHFQAKRGVLIGDAAVGMHPVTAHGFNLGLESACSLGELLQQAAAKQQD
                     IAAKSLLQWYEMKHMLRTRPLYHGTHAMVSLFTNDAPPAKLLRHAVLRVSNHLPPLKK
                     AIAMQLTGKWA"
     gene            2447..2914
                     /locus_tag="PK856_07200"
     CDS             2447..2914
                     /locus_tag="PK856_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02191.1"
                     /translation="MHKRPVNTDPSLTLHLLEQQLRSADDDERFAAMRDLLPLFHAQA
                     QQTLLQVLRYRYLMPTAWIRARLLALSLPQGDIALRADERYHLREVLAALDLWDNEAY
                     LPVLLAHLNELLSHSQTASPLLQECCEVLLSVAPADLHTALQQHGLRLADVLT"
     gene            3051..>3697
                     /locus_tag="PK856_07205"
     CDS             3051..>3697
                     /locus_tag="PK856_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRL02192.1"
                     /translation="MTDYIRIRGARTHNLKNIDLDIPRHQLVVITGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTSHNPRSTVGTV
                     TEIHDYLRLLFARVGTPHCPDHDIPLSSQTVSQMVDSVLAMPEDTKIMVLAPAVSGRK
                     GEFVELFNDLRVQGFTRVRIDGETYSLDDELPVLNKNRKHHIDVVIDRIKVRPDM"
BASE COUNT          846 a         1041 c          934 g          876 t
ORIGIN      
        1 gccttgaccg cattcgccgc caaatttata ttcaagcttt cattccaaaa ggttggctag
       61 catgacttca gtatcaaacc ccatcgcttt ggtcacaggc ggctcacgcg gtataggtgc
      121 ggaaattgcg cagcgcttgg cgcaagacgg ctttaccgtt gccgtgaatt acaccagtaa
      181 tcctgccgcc gcagacgaag tggtggcgca gattacagct gatggcggca aggcttttgc
      241 ggtgcaagcc gacatcagcg atgcggcagc ggtggcggcc atgtttgacg acatcgccaa
      301 acgcggcgac ttgacggtat tggtcaacaa cgctggcatc atgaatttgg cccccatcgc
      361 ggagatggac agcggcgtgt ttgagcgcat gctcaacatc aatttattgg gcagcttcaa
      421 caccatgcag caagccgcca aacacatggt tgcaggtggt cgcgtcatca acacttccag
      481 cagcgtcacc aagctgcttc agcccactta cggcccgtat gccgccacca aagcggcggt
      541 agaagccatg agcgtggtgt ttgcgcgcga attgcgcggc cgcagcatca ccgtgaacac
      601 catcgcccct ggccccactg cttcggcctt gtttttaaat ggcaaaaccc ctgagcaaat
      661 tcagcacttg gaaaacattt ccccgctgca gcgcttgggc cagcccagtg acattgccgc
      721 ggtggtgtcg tttctcgcgg gccccgatgg cgcttgggtc aacggccaaa ccttgtttgc
      781 caatggtggc gcgatttaaa ccgctgtttg ctcaaacaag cggcctgtaa acacttacag
      841 gccgcttgat tttgataatg ggtgtatctg tttgatggca tggaaaaaat cgcagagcca
      901 tcaacacacc gattcaaacc cagctgttcg cggttaatcc acatgaaata cggtctgtaa
      961 ctatttacag gccgcttatt ggtattgcca actgttataa acagatttaa acagacttta
     1021 agcccatttg cctgtgagct gcatggcgat ggcttttttc agcgggggca aatgattgct
     1081 gacgcgcaac actgcatggc gcaacaattt ggcaggcggc gcatcgttgg tgaacagcga
     1141 caccatcgca tgtgtgccgt gatacagtgg ccgcgtgcgc agcatgtgct tcatttcata
     1201 ccattgcaac agcgatttgg ccgcaatgtc ttgctgtttc gccgccgctt gttgcagcaa
     1261 ttcgcccaaa ctgcaagcgc tttccaaacc caaattaaac ccgtgggcgg taacgggatg
     1321 catgccgaca gcggcatcgc ctatcaacac accgcgcttg gcctgaaaat ggcgcgcgtg
     1381 cacgcccacc aatgggtatt ggtgcacgct gctgatgact tgcatcttgc ccaagcgacc
     1441 ttgtaattgc tgcgccatgt cttgcgccaa ggcttcaggc gataaggcca gcaagcgttt
     1501 ggcgatttga tgattggcgg tcaccacaca attggtcatg tggctgctca atggcaacaa
     1561 ggccaaggtg cggccgtaat gaaaacactc aaacgccgtg gcttggttgc tgatttcatg
     1621 ctcggtgcga aacaccaaca cggtgcggcc gtaatcatgg ctgtcggcgg aaatacccaa
     1681 ttggcggcgc gtgtttgaaa aacggctgtc ggccgccacc accaaacgcg tgtgcaagct
     1741 gcgaccgtcg ctgagtttca ccgtggcctc atcatcggcc acttgcactt gcacttgctc
     1801 cacattcacc cccgtgagcc aagccacgtt ttccaccggt tcagcggcat tgaatgcggc
     1861 ctgtcggatg ttgtgattgg acaccaaaaa acccagctgc tcggcaatca agcccgcctc
     1921 aggcttgggc tgagggaaat gcaaggtaaa atcagacgcg ccgctcacca ctttcgcatc
     1981 gcgcagcggg tagatttcgt tttcaggcag gtgttgccac aggccatatt gctgcatcag
     2041 cgcttgcgag gcatgagtta aggcgatttc gcggccatcg tatgtaggat gagcgatgct
     2101 gctgtaatca ttgcgctcca ccagcgccac gctcaagcca ctgcctgcca atgcgcgtac
     2161 caaactcaag cccgctggcc ctgcgcccac caccacgatg tctgtttgca tgctgcttcc
     2221 ctttttcatg ttgaattcag aaagcatcag cttaatgcgc ctaacacaaa cgcgcacaaa
     2281 aatcacgccc aaacttggct tgcatcaaaa gatggagccc tttgtgtaca tggcttttga
     2341 tgaccaacgg atttgggcaa atctgctcat ggcttgattg gcccattttt gatataccca
     2401 tttgtttgct acagtgtgtt cccatctttc taccgatggc atcaacatgc ataagcggcc
     2461 tgtaaacact gaccctagcc tcactctcca cttacttgag caacaattgc gctcagccga
     2521 tgatgacgag cgtttcgccg ccatgcgcga cttgctgcct ttgtttcatg cacaagcaca
     2581 gcaaacctta ctgcaggtgt tgcgataccg ttatttgatg cccacagctt ggatacgcgc
     2641 ccgtttactg gctttgtcac tgcctcaagg cgatattgcc ttgcgagccg atgaacgcta
     2701 ccacttgcgc gaagtgcttg cagccttgga cttgtgggac aacgaagcgt atctgcctgt
     2761 cttgttggcg catttaaacg aattattgtc ccactcacaa acagcaagcc ctttattaca
     2821 agagtgttgt gaggtgttac tgagtgtggc acctgctgat ttgcacacag ctttacagca
     2881 acacggcctg cgtttggccg atgtgttgac gtaatcctcg tggccttact caccgctatg
     2941 gtgtttgaaa taacacagtt ttacctagtc cacatccaca cttaagcgag aagtatcaaa
     3001 gcaaaactgc tataatgctc ggttccgtac cctatagact ccaccacatc atgactgact
     3061 acatccgcat acgcggcgcc cgtactcaca atttaaaaaa catcgatttg gacattcccc
     3121 gccaccaatt ggtggtgatt actggtttgt ctggcagtgg gaaatcttcg ctcgcgtttg
     3181 acacgcttta tgccgaaggt cagcgccgtt atgtggaaag tctgtcggct tacgcccgcc
     3241 aattcttgca aatgatggac aagcctgatg ttgatttgat tgagggtctg tcgccagcga
     3301 tttccatcga gcaaaaatcc acctcgcaca acccgcgctc cacagtgggc acggtcacag
     3361 aaattcacga ttacttgcgc ttgttgttcg cgcgcgtggg cacgccgcat tgccccgacc
     3421 acgacattcc cttgtccagc caaaccgtgt cacaaatggt ggacagcgtg ttggccatgc
     3481 ctgaagacac caaaatcatg gtgttggcgc ctgcggtatc gggccgcaaa ggcgaatttg
     3541 tggagttgtt caatgatttg cgcgtgcaag gcttcacccg agtgcgcatc gatggcgaaa
     3601 cctattcgtt agacgacgaa ttgcctgtgc tcaacaagaa ccgaaaacac cacatagacg
     3661 tggtcatcga ccgcatcaaa gtacgccccg acatgca
//
LOCUS       DAOMVK010000200         3696 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_7282_length_3696_cov_3.712442, whole genome shotgun sequence.
ACCESSION   DAOMVK010000200 DAOMVK010000000
VERSION     DAOMVK010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7282_length_3696_cov_3.712442"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="PK856_07210"
     CDS             complement(<1..230)
                     /locus_tag="PK856_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02193.1"
                     /translation="MYSFQVKLFAIKIVFFWCSNLAKQCDFKACHAANLTPMMWADNT
                     NFSGNSAYYPVYHSSFIPLWNNYMQIVRLPCQ"
     gene            455..1720
                     /locus_tag="PK856_07215"
     CDS             455..1720
                     /locus_tag="PK856_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRL02194.1"
                     /translation="MEQGTHVCSALSIVHPIELVRGLNAKVYDSEQREYIDFVGGIGV
                     LNLGHCHPRIVAAITEQASQLTHSAFNAVPHRPYLQLVKALSAFVPTHTPFDVLLTNS
                     GAEATENALKIARLKTRRTAVIAFDGGFHGRTLAAVNLNGKVAAYKTQLGPLPGPVYH
                     IPYPSADNEVTVAQAQAALERIISVEVDVNEVAAIIAEPVIGEGGFHLLHSEFAQYLR
                     AFCDQHGIALIWDEIQSGFARTGKRFAFEHLGVEPDLLLLGKSIAGGLPLGAVMGSSA
                     WMEGLPKGSLGGTFSGNALACAAALATIEILSDDTALVHMAQAYEQAVLTHYQHWREQ
                     GLPVGRITGVGTMRGIELVDGAGKPNPALLGKVLQAAREQGLLLMPAGKYRHVIRLLA
                     PLTTEAEVMEQGLQRLGAVLAQCSADVTV"
     gene            1733..>3696
                     /locus_tag="PK856_07220"
     CDS             1733..>3696
                     /locus_tag="PK856_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRL02195.1"
                     /translation="MIPIHVMPEMPTQTFLHVLQQQGFAGEIDTSAAQRAVYATDNSI
                     YERPPQAIVCPKHVADLQVLTRLLEEERFRGVVVTARGGGTGTNGQSLTEGIVVDVSR
                     HMNHILSIDVAKRQVRVQAGVVKDQLNRALKPHGLFFAPELSTSNRATIGGMVNTDAS
                     GQGSCTYGKTRDHVLALKTVLWGGEVLESQALPEQTWRNDITSLSANMQQRFHMLYEL
                     VSEHQEAIQAAFPKLNRCLTGYDLPHVLEQGQFNLNSVLCGAEGSLGLVAEATLNVLP
                     IPTERVLVNISYAHFQEALRDASALMAMQPLSIETVDSKVLGLAKADIVWQQVAEFFP
                     EVAGQSAVMGINLVEFVGDDVPVLQAQVARFVAHLQADAAVKRLNITEAWGSAAVERV
                     YAMRKRSVGLLGNVEGERRPQPFVEDTAVPPENLAAYIAEFRALLDGMQVDYGMFGHV
                     DAGVLHVRPALDMKNPQDAAKIKPITDQVAALVHKHGGVLWGEHGKGLRSEYAPQFFG
                     AVYPLIQQVKAIFDPYNQFNPGKIATPSTQDHAVLTRVDEVSLRGQLDQVIKPEVWHS
                     FHNAVHCNGNGACYNYDENDAMCPSWKAMRDRVHSPKGRASLMKTWLRQQGDVLGEEQ
                     AIQSQSVFQNVMQQLFLRLQAAPTREQDDFS"
BASE COUNT          843 a          883 c         1086 g          884 t
ORIGIN      
        1 atttggcacg gcaaacgaac tatctgcatg taattattcc ataaaggaat gaagctcgag
       61 tggtagacag ggtaataggc tgaattgccg ctgaaattgg tgttgtcagc ccacatcata
      121 ggggtaaggt tggcggcgtg gcaagcctta aaatcacatt gtttggctaa gtttgaacac
      181 caaaaaaata cgattttaat ggcaaacagt ttaacttgaa atgaatacaa atcagtcttt
      241 cctcatgtgg catcaagtca aattgtgatg gtttacaggc cgctttcatg gccttgtttg
      301 gtatgagttg gaagatttaa gaaaaaaatg aatgaagtgg tggggttttt tcgtttgtcc
      361 aaaccacggg gaatttggtt tgatagcgct tacataacaa cagtcacacc aaccatggtt
      421 gatgacacca cacatcatga aggacggcaa gaatatggaa caaggcaccc acgtttgctc
      481 ggcattgtct attgtgcacc caatcgaatt ggtgcgcggc ttgaatgcca aggtgtatga
      541 cagcgagcag cgcgaataca ttgattttgt tggcggcatc ggcgtgttga atttgggcca
      601 ttgccatcca cgcatagtgg ccgccatcac cgagcaagct tcgcaattga cccattcagc
      661 tttcaatgcc gtgccgcatc gcccgtattt gcaattggtc aaagccttga gtgcttttgt
      721 gcccacccac accccgtttg atgtgttgct gaccaacagc ggcgcagaag ccacggaaaa
      781 cgctttaaaa atcgcccgtt tgaaaacccg tcgcaccgct gtcatcgcct ttgatggcgg
      841 ctttcatggc cgaactttgg cggcggtaaa cctcaatggc aaggtggcgg cttacaaaac
      901 ccagcttggt cctttgcctg gccctgtgta ccacattccc tacccaagtg cagacaatga
      961 agtgactgtg gctcaggctc aggccgcttt ggagcgcatc atcagcgtgg aagtggacgt
     1021 gaacgaagtg gcggccatca ttgccgagcc tgtcatcggt gaaggcggtt tccatttgtt
     1081 acactcagaa tttgcccaat acttgcgcgc gttttgcgac caacacggca tcgccttgat
     1141 ttgggatgaa atccaatcgg gttttgcgcg cacaggcaag cgctttgcgt ttgagcattt
     1201 gggggtagag cctgatttgt tgttgttggg caagagcatt gcgggcggct tgcctttggg
     1261 cgctgtgatg ggttcgtccg cttggatgga aggtttgccc aagggcagct tgggaggcac
     1321 gttctcaggc aatgctttgg cgtgtgcagc ggctttggca acgatagaaa ttttgtctga
     1381 tgatacagcc ttggtacaca tggctcaggc gtatgaacaa gcggtgttaa cgcattacca
     1441 acactggcgt gaacaaggct tgcctgtggg gcgcatcacg ggcgtgggca cgatgcgcgg
     1501 catcgaattg gtggatgggg caggcaagcc caatcctgcc ttgcttggca aagtgttaca
     1561 ggccgccaga gaacaaggtt tgctgttgat gcctgcaggc aaataccgcc atgtcatccg
     1621 tttgcttgcg cctttaacca cagaggccga agtgatggag caaggcttac agcgcttggg
     1681 tgcggtattg gcgcagtgtt ctgctgatgt cacggtgtaa gcgagcggca cgatgatacc
     1741 gattcatgtg atgccagaga tgcccacaca aacctttctg catgtgttgc aacagcaagg
     1801 gtttgcaggc gagatagaca ccagcgcagc gcagcgcgcg gtgtatgcca ctgacaattc
     1861 catatacgag cgcccaccgc aagccattgt gtgccccaag catgtggccg atttgcaggt
     1921 gttgacgcgc ttgttggagg aagagcgctt tcgcggcgtg gtggtgacgg cgcgcggcgg
     1981 cggcacaggc accaatggtc agtccttgac cgagggcata gtggtggacg tgtcgcggca
     2041 catgaatcac attttgagca tcgatgtggc caagcgccaa gtgcgggtgc aagcaggcgt
     2101 ggtgaaagac caattgaatc gggcattgaa gccacacggc ttgttttttg cacccgaatt
     2161 gtccacttcc aatcgcgcca ccattggcgg catggtcaat accgacgcca gtggccaagg
     2221 cagttgcact tatggcaaaa cgcgcgacca tgtgttggca ttgaaaaccg tgttgtgggg
     2281 cggcgaggtt ttggaatcgc aagctttgcc tgagcagact tggcgtaacg acatcacctc
     2341 cttgtctgcc aacatgcaac agcgttttca tatgctgtat gagctggtga gcgaacatca
     2401 agaggcgata caggccgcct ttcccaagct taaccgctgt ttgacaggct acgatttgcc
     2461 gcatgtgttg gagcaagggc aattcaatct caacagcgtg ttgtgcggcg ctgaaggctc
     2521 gctgggcttg gtggctgagg ccacgttgaa tgtgttgccg attccgactg agcgggtgtt
     2581 ggtcaacatc agctatgccc attttcaaga ggctttgcgg gacgcttcag ctttgatggc
     2641 catgcagcct ttgtcgatag aaaccgtcga ttccaaagtg ctgggcttgg ccaaagccga
     2701 cattgtgtgg cagcaagtgg ccgagttttt ccctgaagtg gcaggccaaa gcgcggtgat
     2761 gggcatcaat ttggtggaat ttgtgggcga tgatgtgccc gtattgcaag cgcaagtggc
     2821 gcgttttgtg gcgcatttac aggccgatgc tgcggttaag cgcttaaaca tcaccgaagc
     2881 ttggggcagt gcggcggtgg agcgggtgta tgccatgcgc aagcgctcgg ttggtttgtt
     2941 gggcaatgtt gagggcgagc gccgaccgca gccctttgtg gaagacactg ccgtgccgcc
     3001 tgaaaatttg gcggcttaca tcgccgaatt tcgtgcctta ttggacggca tgcaagtgga
     3061 ttacggcatg tttgggcatg tggatgcggg cgtgttacac gtgcgcccag cgcttgacat
     3121 gaaaaacccg caagacgccg ctaaaatcaa gccgattact gaccaagtgg cggccttggt
     3181 acacaaacat ggtggcgtgt tgtggggtga gcatggcaag ggtttgcgct cggaatatgc
     3241 accccagttt tttggggcgg tttacccatt gatacagcaa gtcaaagcga tattcgaccc
     3301 ttacaaccaa ttcaatcctg gcaaaatcgc cacgccgtct acccaagacc atgcggtgtt
     3361 gacgcgggtg gatgaagtgt cattgcgggg ccaactcgac caagtcatca aaccagaggt
     3421 gtggcacagc tttcacaatg ccgtgcattg taatggcaac ggtgcttgct ataactacga
     3481 tgaaaacgat gccatgtgcc cttcgtggaa ggccatgcgc gacagagtgc attcacccaa
     3541 aggccgcgcc agcttgatga aaacttggtt gcggcagcaa ggcgatgtgt tgggtgaaga
     3601 gcaagcgata caaagccaat cggtgtttca aaacgtgatg cagcaattgt ttttgcgttt
     3661 acaggccgct cctacacgtg agcaagacga cttttc
//
LOCUS       DAOMVK010000201         3682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_7322_length_3682_cov_2.877033, whole genome shotgun sequence.
ACCESSION   DAOMVK010000201 DAOMVK010000000
VERSION     DAOMVK010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7322_length_3682_cov_2.877033"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PK856_07225"
     CDS             <1..487
                     /locus_tag="PK856_07225"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRL02196.1"
                     /translation="VIVIAFVFLLVRLATSGYFADVEEMTGPAVETRIKPQGVVTMGD
                     GVPAGQRTGESVFNKVCIQCHAENAVVANSPKMNHPSDWAPRIAKGMPALLHSAINGF
                     NAMPKRGGNPDLTDAELERAIAYMANSAGGKFTEPAIVAASDATSASAPATASAASAA
                     Q"
     gene            557..1641
                     /locus_tag="PK856_07230"
                     /pseudo
     CDS             557..1641
                     /locus_tag="PK856_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957870.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
     gene            1663..>3682
                     /locus_tag="PK856_07235"
     CDS             1663..>3682
                     /locus_tag="PK856_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HRL02197.1"
                     /translation="MKKKDTRKPTLTPPKRGVVSNNKLSKQPDIGFRVFSAFNDTIWL
                     IMLIVTGFFAAALISFDMNDPAFTRSTDGTTAHNMTGMVGAYVADIAYYLCGLSIWWV
                     VIEAAILLFRAFKPLLIAKKIDTNHRLGMGALGTVIMIAGSGLLETWLGSAYLQDRLP
                     LGAGGFWGQTIVPILEYGVGRSSGVIISMLLMLMGLSMMLQMTWLELVEKVGNRAQWL
                     WFNLIKRPSQLMRELPKPRKFRQTMATVEDITATPVATPTGASSNRKTPTPNADAAAF
                     DTAPTVAPPSKPAIPVQTSLFDDEPVKKPANNHPVRTDLPHLDLLQAATAQVSTMKPE
                     QLQETAQLIETKLAEFGVSVQVVSATTGPVITRYEIEPATGVKGSQIVNLAKDLARAL
                     SLQSVRVVETIAGKNTMGIELPNESRQDVAFRELLQANVFDSAKSKLTLAMGKDIAGV
                     PVVADLGKMPHVLVAGTTGSGKSVAINTMILSMLYKATAEEVRLIMVDPKMLELSVYE
                     GIPHLLAPVVTDMKQAGNALSWCVAEMERRYRLLAHVGVRQLAGFNDKIQAARAADKP
                     LFNPFSLTPDEPEALEKLPHIVVIIDELADLMMVEGKKVEQQIARLAQKARAAGIHLI
                     LATQRPSVDVITGLIKANIPTRIAFQVSSKIDSRTILDQGGAENLLGKG"
BASE COUNT          873 a          974 c          960 g          875 t
ORIGIN      
        1 cgttattgtt atagcatttg tgtttttgct ggtgcgtttg gccacttcgg gctattttgc
       61 cgacgtggaa gaaatgacag gccctgcagt tgaaacgcgc atcaagccgc aaggcgtggt
      121 caccatgggt gacggcgtgc cagcgggcca acgcacaggc gaatcggtgt tcaacaaagt
      181 gtgtattcaa tgccatgcgg aaaatgccgt ggtggccaat tctccgaaaa tgaatcatcc
      241 aagcgattgg gcgccacgca tcgccaaagg catgccggct ttattgcatt cggccatcaa
      301 cggtttcaat gccatgccca agcgtggtgg taaccctgat ttgaccgatg ccgaattgga
      361 acgcgcgatt gcctacatgg ccaattcagc gggcggtaaa tttaccgagc ctgcaatcgt
      421 ggcggcctct gatgccacca gtgccagcgc acctgccacg gccagcgcag cctcagccgc
      481 ccaatagacc caagatgtag tatgataaac gcgccgaaaa gttggcgcgt tttttattga
      541 ttaaagaaga taatccatga cttattatat cggtttgatg tcgggcacca gtttggatgg
      601 cgtggatgcc gtgctcattc aaatggatgg tgccgcttgg cagggtgcat tggctcacag
      661 ctatctgcct taccctgacg acatcaaaac cgcggttttg gccttacaac aacgtggaca
      721 cgatgagttg caccaagcag ctgtgttgtc acaacgcttg agtcgaattt atgctcaaac
      781 cgtgctgcaa ttgttggctg agcaagactt acaggccgct gatattgccg ccataggctg
      841 tcatggccaa accatacgcc atgcacctga gttgggttac agcttgcaaa ttgccgattg
      901 gtcgttgtta gcggaattga ctggcatcaa cgtgatcggc gattttcgca gccgcgattt
      961 ggccgcagga ggcaaggcgc acccttggtg ccggcttttc atgccgcttt gtttacccat
     1021 cctgaagaaa cgcgcgtgtt ggccaatata ggcggcattt ccaatatcag cgtgttaagg
     1081 ccaaacagcc cgattttggg ttttgacact ggcccaggca atatgttgat ggatgcttgg
     1141 atgcaaaccc atttcaatct gccttacgat gccgacagct gcatcgccaa acaaggcaaa
     1201 gttttgccag aattgctaca aatattgtta agccatccct attttcacca agcagcgccc
     1261 aaatccacag gccgcgattt gttttctttc aactggttag cccaatattt gaatggcaca
     1321 gaagccccac aagatgtgtt gcgcacccta ttggagctga ctgcggtgag tttaagcacg
     1381 gaaattgctg gttttgcccc caaagcagtg tatgtgtgtg gcggtggtgt gtacaatccc
     1441 ctgctactag agcgattacc tgtattgttg ccacaaagcc aatggcacag taccactgcc
     1501 ttgaatctag acccacaatg ggtcgaagct gctgcttttg catggttggc agcctggcgc
     1561 ttgcttggct tacccagcaa tgctcctgat gccactggtg caaaagggcc gcgcgtattg
     1621 ggtgccattt accaagcctg atttttattt tgatgtaagt gtatgaagaa aaaagatacc
     1681 cgtaaaccca cgttaacccc gcctaagcgg ggcgtggtca gcaacaataa attgtctaag
     1741 cagcccgata tcggcttccg cgtattcagt gcttttaacg acaccatttg gttaatcatg
     1801 ttaatcgtca cgggcttttt tgccgcggcc ttaatcagct tcgacatgaa cgacccagcc
     1861 tttacccgca gcaccgatgg caccactgcc cacaacatga cgggcatggt gggcgcttat
     1921 gtggccgaca ttgcctatta tttgtgcggc ttgtcgattt ggtgggtggt gatagaagcc
     1981 gctattttgc tgtttcgtgc cttcaaaccc ttgctcattg ccaaaaaaat cgacaccaac
     2041 caccgcctcg gcatgggtgc cttgggcact gtcatcatga ttgcaggcag tggcttgctt
     2101 gaaacctggt tgggctcagc ctatttgcaa gaccgtttgc ctttgggcgc gggcggcttt
     2161 tgggggcaaa ccatcgtccc catcttggaa tacggcgtgg gccgctccag tggcgtcatc
     2221 atcagcatgt tgttgatgct gatgggctta tcgatgatgt tgcaaatgac atggttggaa
     2281 ttggtggaaa aagtgggcaa ccgtgctcaa tggctgtggt ttaacctcat caagcgcccc
     2341 agccaattga tgcgcgaatt gcccaagcca cgcaaattcc gccaaaccat ggccacagta
     2401 gaagacatca ccgccacccc cgttgccacg cccacaggcg cgagcagcaa ccgcaaaact
     2461 cccactccca atgcggatgc cgctgcattt gatactgcgc caacagtcgc gccgccaagc
     2521 aaacctgcca tccccgtaca aacctcgctg tttgatgatg agccagtgaa gaagcccgct
     2581 aacaaccatc ctgtgcgcac cgatttgccg catttggact tgttacaggc cgctacagca
     2641 caggtttcca ccatgaagcc tgagcaattg caagaaaccg cgcaattaat cgaaaccaaa
     2701 ttggccgagt ttggcgtgag cgtgcaagtg gtcagtgcta ccacaggccc tgtgattacc
     2761 cgttatgaaa tcgagcctgc cacaggcgtc aaaggcagtc aaattgtcaa tttggccaaa
     2821 gatttggcac gggctttgtc tttgcaaagc gtgcgcgtgg tggaaaccat tgcgggcaaa
     2881 aacaccatgg gcatagagtt gcccaatgaa tcgcgccaag atgtggcgtt tcgcgaattg
     2941 cttcaagcga atgtgtttga ttcagccaaa tccaaactca cgcttgccat gggcaaagac
     3001 attgcaggag tgcctgtggt ggctgatttg ggcaaaatgc cccacgtttt ggtggcaggt
     3061 accacgggtt cgggcaagtc ggttgccatc aacaccatga ttttgtccat gctctataag
     3121 gccacggccg aagaagtgcg cctcatcatg gtagacccta aaatgttgga attgtcggtg
     3181 tacgaaggca tcccccattt gctcgcccct gtcgtcaccg acatgaagca agccggcaac
     3241 gccctcagtt ggtgtgtggc cgaaatggag cgtcgctacc gcttgctcgc acacgtgggt
     3301 gtgcgccaat tggcaggctt taatgacaaa atacaggccg ctcgtgcagc cgacaagcct
     3361 ttatttaacc cattcagcct cacgcccgat gaaccagaag ccttggaaaa attgccgcac
     3421 atcgtggtca tcattgatga gttggccgat ttgatgatgg tggaaggcaa aaaagtggag
     3481 caacaaattg cccgcttggc gcaaaaagcg cgtgctgcag gtatacactt gattttggca
     3541 acacaacgcc catcggtaga cgtgatcaca ggcttgatta aggccaacat ccctacccgc
     3601 atcgctttcc aagtatcgag caaaatcgat tcgcgcacca tcttagacca aggcggcgcg
     3661 gaaaacctgc tcggcaaagg cg
//
LOCUS       DAOMVK010000202         3679 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_7332_length_3679_cov_3.338024, whole genome shotgun sequence.
ACCESSION   DAOMVK010000202 DAOMVK010000000
VERSION     DAOMVK010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7332_length_3679_cov_3.338024"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /gene="mraW"
                     /locus_tag="PK856_07240"
     CDS             <1..237
                     /gene="mraW"
                     /locus_tag="PK856_07240"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HRL02198.1"
                     /translation="RGRLAVISFHSLEDRIVKQYFKSLVEKPKLPRWAMVKDEDLPAA
                     PIELMGKAIKPSAEEISANPRSRSAIMRVVEKTN"
     gene            246..506
                     /gene="ftsL"
                     /locus_tag="PK856_07245"
     CDS             246..506
                     /gene="ftsL"
                     /locus_tag="PK856_07245"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HRL02199.1"
                     /translation="MNKLNVLLLVAVVASAFALVTLHYENRVLYTQIAKEQQTAYQLN
                     EEHAVLILEQAKFTNHHVIQEAAAKQNLHVPSLAETKMIEKE"
     gene            509..2260
                     /locus_tag="PK856_07250"
     CDS             509..2260
                     /locus_tag="PK856_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009118966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRL02200.1"
                     /translation="MLIKNEYKPEMIGVKAKKIKKVQSTNGRLFVVLGVLGLAFVAAC
                     VKGIYMQTQQQSFLQNQSNQRVVRNLPLPASRGTITDRNGSVLALSVPTRYVSADPSK
                     LEQLPTAEQLQQLSTILNVPVVELTSKLSDKKRQFVYLKRQLPLDQAEKVNALKIKGI
                     HLVEDSKRYYPMGASFAHVLGFVNIDDKGQEGLEMALNKSLSGVNGIETVMVDKYGTV
                     FSAVDSDENRPAQNGQDLHLSLDQRIQAMAHDSLLKTLEFHKAKAGSAVVLDAKTGEI
                     LALVNAPTFDPNNPSEAKPEQRRNRAVIDMVEPGSTMKPIPIALALDQGKVTRNTVLN
                     TMPYNIGPKTIKDTHNYPSLTVQGVIQKSSNVGSSKMSAMFKPKDMYDFYSKVGYGRK
                     LETGFPGESVGKLRPWDKWYPVDQATMSYGYGIQVSVLQLARAYTMFTNNGAILPVTF
                     RKLDHAPEGQQLIKPETAQAMREMMVSVTEQGGTGVRGAVDGYDVAAKSGTAQKYTPG
                     VGYTSNKHIALFAGFAPAQNPRVVVVVSIDEPSANGYYGGLVAGPAFQEIMAGSLNVL
                     GVAPTRPMHVAAAKPTQ"
     gene            2306..>3679
                     /locus_tag="PK856_07255"
     CDS             2306..>3679
                     /locus_tag="PK856_07255"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007341900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRL02201.1"
                     /translation="MNWSALGLTAPIRVVIDSRQLHAGDVFLACPGEYTDGRDFIQAA
                     IDAGAAAVIWDTDGGFEWTFGATPNISISSLRQYMGLIAAEALNHPGQQLDIIGVTGT
                     NGKTSITQWLAQALDQLGQPCGIMGTVGNGFWDKLEESSHTTLDAWSVEQWLHQFVQQ
                     GAKAVAMEVSSHGLDQYRVNGVPFTSAIFTNLTRDHLDYHGSMDAYGACKSRLFFWQG
                     LKHAIINVDDDFGKQLITEIAHSDSEHAQVYGYGFNETADVSIHTYQPSLDGTHIVMH
                     TPWGVANIHTQLIGRFNAQNLAACVAVLGAQGHELGAICQALANIKPATGRMDGIREA
                     GKPLVVVDYAHTPDALEKAVSTLHDLKQTASKVWCVFGCGGNRDKGKRPLMGAAAATH
                     ADVVVVTSDNPRLEAPQDIINDILPAVPNPAYVHTDRAQAIQYAVQNASANDIILIAG
                     KGHETYQD"
BASE COUNT          994 a          796 c          960 g          929 t
ORIGIN      
        1 agagggcgtc ttgctgtgat ctcattccat tctttagaag atcgcattgt taaacagtat
       61 tttaaaagtt tggtggagaa accaaagcta cctaggtggg caatggtgaa agacgaagat
      121 ttgccagctg cacccattga attgatgggt aaggcaatca agccttctgc tgaggaaatt
      181 tcagctaatc cacgttcccg ttccgccatt atgcgggtgg tggaaaaaac gaactgaagt
      241 ggcatatgaa taaattaaat gtgttgttgc tggttgcggt ggtggcttca gcatttgctt
      301 tggtcacgct gcattatgaa aatagggtgt tgtatacgca aattgccaaa gagcagcaaa
      361 cggcgtatca attgaatgaa gagcatgcgg tattgatctt ggaacaggcg aagtttacca
      421 accaccatgt gatacaagag gcagctgcaa agcagaatct acatgttcca agccttgctg
      481 aaaccaaaat gatagaaaaa gaataatcat gctgattaaa aacgaataca aacctgaaat
      541 gattggtgtc aaagccaaga aaatcaagaa agttcaatct accaatggtc gattgtttgt
      601 ggtgttgggt gtgttgggtt tggcgtttgt tgcggcttgc gtgaaaggca tttacatgca
      661 aacgcagcaa cagtcgtttt tgcaaaatca aagcaatcag cgggttgtac gcaatctgcc
      721 tttgcccgcc agtcgcggca ccattaccga tcgcaatggc tctgtattgg ctttaagtgt
      781 gccaacacgc tatgtttcag ctgacccatc taagttggag caattaccca ctgcagaaca
      841 attgcaacaa ttgtccacga tattgaatgt gcctgttgtt gaattgacca gcaaattgag
      901 tgataaaaag cgtcagtttg tgtatttgaa gcgtcagtta cccttagatc aggctgaaaa
      961 agtcaatgcc ttaaaaatca agggtataca cttggttgag gattcaaagc gttattaccc
     1021 tatgggtgcg tcgtttgcac atgttttggg ctttgtgaac atcgatgaca aagggcaaga
     1081 aggtttggaa atggctttga ataagtcttt gtcgggtgtg aatggcatag aaaccgtgat
     1141 ggtggacaaa tacggtacgg tctttagtgc ggtggacagt gatgaaaatc gacccgcaca
     1201 gaatggccaa gatttacacc tgtcactaga ccagcgcata caagccatgg ctcatgacag
     1261 tttgttaaaa acattggaat tccataaggc taaagcaggc agcgcagtgg tgttggatgc
     1321 caaaacgggt gaaattttgg ccttggtgaa cgcacctacg tttgatccta acaatccttc
     1381 agaagccaag cctgagcagc gtcgcaatcg cgcggtgatt gatatggtag agccaggttc
     1441 aaccatgaaa cctattccca tagctttggc attagaccaa ggtaaggtga cgcgcaatac
     1501 ggtgttgaat accatgccgt ataacatcgg ccctaaaacc attaaagaca ctcacaatta
     1561 tccaagctta accgtgcagg gcgtgataca aaaatcgtcc aatgtgggct ctagcaaaat
     1621 gtcggcaatg tttaagccta aagacatgta cgatttttat tctaaagtgg gttatggccg
     1681 caagttggaa acgggcttcc ctggtgagtc agtgggtaaa ttgcgtcctt gggataaatg
     1741 gtatccagtg gatcaggcga ccatgtcata tggctatggc atacaagtga gtgtgttgca
     1801 attggcacgt gcttacacca tgttcaccaa caatggtgcg attttgcctg tgactttccg
     1861 caagctcgat catgctccag aagggcaaca actgattaaa cctgaaaccg cacaagcaat
     1921 gcgtgaaatg atggtatcgg tgactgagca aggcggtacg ggtgtgcgcg gtgcggtgga
     1981 tggttatgat gtggcagcca aatcaggtac ggcacaaaaa tacacaccag gcgtgggtta
     2041 taccagcaat aagcacattg ctttgtttgc gggctttgct cccgcccaaa atccacgcgt
     2101 tgtggtggtg gtaagcattg atgagccgag tgcaaatggc tattatggcg gcctggttgc
     2161 aggaccagct tttcaggaaa tcatggcagg cagtttgaat gttttgggtg tggcaccaac
     2221 cagacccatg catgtggctg cggccaaacc aacacagtag ccatcaatta tgtttagtgc
     2281 aaaaacgcct ttgaatccgc tggccatgaa ttggtcagct ttgggactaa ccgctccaat
     2341 acgagtggtg atagacagcc gccagctaca tgctggcgat gttttcttgg catgcccagg
     2401 tgaatacacc gatgggcgtg attttattca ggccgctatt gatgcaggtg cggccgccgt
     2461 gatttgggat accgatggtg ggtttgagtg gacgtttggt gccacgccca atatcagtat
     2521 ctcttctttg cgccaataca tgggtttgat tgccgctgag gccttgaatc accccggcca
     2581 acaattggac atcattggtg tcacaggcac caatggcaaa acatccatta cacagtggtt
     2641 ggcgcaggct ttggatcaat tgggtcagcc gtgcggcatc atgggtacgg tgggcaatgg
     2701 tttctgggat aaattagaag agtcttctca taccactttg gatgcttggt cggttgagca
     2761 atggttgcat caattcgtgc agcaaggcgc caaggcggtg gctatggaag tgtctagcca
     2821 tggtttagac caataccgtg tcaacggtgt gccgtttacc agtgccatct tcaccaattt
     2881 aacccgtgac catttggact atcacggcag tatggacgcc tacggagctt gcaaaagccg
     2941 tttgtttttt tggcaaggat tgaagcatgc catcatcaat gtggatgatg attttggcaa
     3001 gcagctgatt actgaaatcg cccattccga ttcagaacac gcacaagtgt acggctatgg
     3061 gttcaatgaa accgccgatg tcagcatcca tacttaccaa ccgagcttgg atggcacgca
     3121 cattgtcatg cacacacctt ggggtgtggc caatatccac acccagttga taggtcgctt
     3181 taatgcacaa aaccttgcag cttgtgtggc agtattgggt gcacaaggcc atgaattggg
     3241 tgcgatttgc caagctttgg caaacatcaa gcctgctaca ggccgcatgg atggcatacg
     3301 cgaagcgggc aaacctttgg tggtagtgga ctatgcccac acaccagatg ccttggaaaa
     3361 agccgtttcc accttacatg acttgaaaca aaccgcttca aaagtatggt gtgtgttcgg
     3421 ttgtggtggc aatcgcgaca aaggaaagcg acctttgatg ggcgctgcgg cagccacaca
     3481 tgctgatgtg gtggtggtga ccagtgacaa tccacgcttg gaagcgcctc aagacatcat
     3541 caacgatatt ttgccagccg tgcctaatcc agcctatgtg cataccgacc gagcacaagc
     3601 gatacagtat gcggtgcaaa acgcttcagc caacgacatt attttaatcg caggcaaagg
     3661 ccatgaaacg tatcaagac
//
LOCUS       DAOMVK010000203         3669 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_7358_length_3669_cov_3.795794, whole genome shotgun sequence.
ACCESSION   DAOMVK010000203 DAOMVK010000000
VERSION     DAOMVK010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7358_length_3669_cov_3.795794"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..349)
                     /locus_tag="PK856_07260"
     CDS             complement(29..349)
                     /locus_tag="PK856_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HRL02202.1"
                     /translation="MAYFERHVFVCTNARNDDSRKSCGDHNVGKHAAGVVKNECLKAH
                     IHGEGKIRVSQSGCLGRCEDGPLLVIYPEARWYNYHSDEDLKHIVAEDLVAGRPVEHL
                     LLDK"
     gene            complement(371..1165)
                     /locus_tag="PK856_07265"
     CDS             complement(371..1165)
                     /locus_tag="PK856_07265"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HRL02203.1"
                     /translation="MTRHPYQRLRAQQFELPEVGISEEDNIRSLHLGSPTIQSSMNLD
                     DPSELVLSYSQAMMGWLLFQDPPAHITQIGLGGGSFARWLAEFCPVTLQTVVEIHPQV
                     IAIAQAMFEIPAESDTFELVEADGADFIKTMRGGTDVIMVDGFDGEQIIDALVDVPFF
                     QDCHTALTEQGIFVTNWWQGDKRFDHFVEKLRIVFDDRVLLLPALTHGNIAVFAFKDT
                     PEMNLDKLKKRADKLQETYHLDFAHMVHSIKSRHQHNGKVLQFTPA"
     gene            complement(1315..1704)
                     /locus_tag="PK856_07270"
     CDS             complement(1315..1704)
                     /locus_tag="PK856_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02204.1"
                     /translation="MRITRLGVVCVAVALQAACLSTTVSDDAQALAQLQQDKARLNRV
                     YASVEASLLEIQRSDAAMQYARQQLAELQLARLKTCTSPASPNTQLRLQYAKCQYQAT
                     LSDLDKLQQWQKVRYQFSAKTAKTASK"
     gene            complement(1695..2621)
                     /gene="ttcA"
                     /locus_tag="PK856_07275"
     CDS             complement(1695..2621)
                     /gene="ttcA"
                     /locus_tag="PK856_07275"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HRL02205.1"
                     /translation="MSEKSKAELEANKLMKRLRHAVGDAINDFGMIEHGDKIMVCLSG
                     GKDSYAMLDILLGLQKSAPIDFDIVAVNLDQKQPGFPTHVLPEYLEKLGIAYHIIEED
                     TYSVVKRVVPEGKTTCALCSRLRRGVLYRVAKELGCSKIALGHHRDDILETLFLNMFF
                     GGKLKSMPPKLVSDNGEHIVIRPLAYVKEKDLVRFAALRQFPIIPCNLCGSQPNLQRQ
                     VVKEMLEDWDKRFPGRIESMFSALQNVVPSHLADPKLFDFVGLERGQDLPHGGDTAFD
                     KEEFKSNEPAPLGRVINILDNRSPKAEPLSCE"
     gene            complement(2805..3248)
                     /locus_tag="PK856_07280"
     CDS             complement(2805..3248)
                     /locus_tag="PK856_07280"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRL02206.1"
                     /translation="MPQLHYSIQIDQDPLTVHNVLVDKEAYATWTYSFSNASCFDGDW
                     SEGSQMLFTFVGANQTQWGMVARVDKNQPGELIQLRYVDQWKNNPQTPHNWNSALETY
                     RFIPISQGTKLSCTLEVDNNEACIEFFDHAWPAALQRLKDLCEQQ"
     gene            complement(3338..>3669)
                     /locus_tag="PK856_07285"
     CDS             complement(3338..>3669)
                     /locus_tag="PK856_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HRL02207.1"
                     /translation="KQRDPTCHGEVVAIRDACKNLNSHDLSGAMLYTTAEPCPMCLGA
                     ILWANIEQVYYGCNIQDTDNIGFRDEVFYRVATPEGKAKLLQECERASCLDLFQEYSE
                     LEHKQLY"
BASE COUNT          820 a          874 c         1003 g          972 t
ORIGIN      
        1 ccacacgatt tcaggttgtt tcaacatatt atttgtccaa taacaggtgt tctacagggc
       61 ggcctgcgac caaatcttcg gcgacaatgt gctttaaatc ctcatcgctg tggtagttat
      121 accagcgtgc ttcgggataa atcaccaaca aagggccgtc ttcacagcgc cccaagcagc
      181 cactttggct gacgcggatt ttgccttcgc catgaatgtg ggctttgagg cattcgtttt
      241 tcaccacgcc tgcggcgtgt ttgcccacgt tgtggtcgcc gcaagatttg cggctgtcat
      301 cgttgcgggc attggtgcac acaaacacat gtcgttcaaa ataagccatg gttatccttt
      361 tacaggccgc ttaagccggt gtgaattgca atactttgcc attgtgttgg tggcgtgatt
      421 tgatggaatg caccatgtgg gcaaaatcca aatggtaggt ttcttgcagc ttgtcggcgc
      481 gttttttcaa tttgtccaaa ttcatttcag gcgtgtcttt gaacgcgaac accgcaatat
      541 tgccgtgggt gagggcaggg agcaagagca cacggtcgtc aaacacgatg cgcagttttt
      601 ccacgaaatg gtcgaaacgc ttgtcgcctt gccaccaatt ggtgacaaaa atgccttgct
      661 cggttaaagc cgtgtggcaa tcttggaaaa atggcacatc taccaaggcg tcgatgattt
      721 gttcgccatc gaagccgtct accataatca catcggtgcc gccgcgcatg gttttgatga
      781 aatcggcacc gtctgcctcc acgagttcaa acgtgtcgct ttcggcgggg atttcgaaca
      841 tggcttgggc aatggcgatg acttgcgggt ggatttccac cacggtttgc aaggtgactg
      901 ggcaaaattc agccaaccag cgggcaaacg agccgccacc caaaccgatt tgggtgatgt
      961 gggctggcgg gtcttgaaac agcaaccaac ccatcatggc ttggctgtaa gacaagacca
     1021 attcgctggg gtcatcgaga ttcatcgacg actgtatggt cgggctgccc aaatgcaatg
     1081 agcggatgtt gtcttcttcg gaaatgccca cttcgggcaa ttcaaattgt tgcgcccgta
     1141 agcgctgata cgggtgacgg gtcatggtct tgtacaggca aaataatgcc aacactataa
     1201 cacaggcaca gggctgggtt ggtgagtgag ggctggggtt aaagaatggg gtttgcagtg
     1261 tggctgcaag cgtttacagg ccgcttgtat tttaataagc ggcctgtaaa cggtttattt
     1321 actggcggtt ttggcagtct tggcgctgaa ttggtagcgc actttttgcc attgttgcaa
     1381 tttgtccaag tcgctcaaag tggcttggta ttggcacttg gcatattgca aacgcaattg
     1441 ggtattgggc gatgcgggcg aggtgcaggt tttgaggcgg gccagttgca gctccgccaa
     1501 ttgttgccgc gcgtattgca tggccgcatc gctgcgttgg atttccagca aactggcttc
     1561 gacgctggca tacacgcggt tcaggcgcgc tttgtcttgt tgcagttgcg ccaatgcttg
     1621 cgcatcgtcg ctgacggtgg tggataggca agcggcctgt aaagccacgg ccacacacac
     1681 cacacccaat cgcgttattc gcatgacagc ggttccgctt tggggctacg gttgtccaaa
     1741 atattgatga cgcgacctag gggcgcgggc tcgttgcttt tgaactcttc tttgtcaaac
     1801 gcggtgtcgc cgccgtgagg caagtcttgg ccgcgctcta ggccaacaaa atcaaacagt
     1861 tttgggtcgg ccaaatgcga aggcaccacg ttttgcaagg cagagaacat cgattcaatg
     1921 cgccctggaa aacgtttgtc ccaatcttcg agcatttctt tcaccacttg gcgctgcaaa
     1981 ttgggctggg agccgcacaa attgcacgga atgatgggga attggcgcaa ggcggcaaag
     2041 cgtaccaagt ctttttcttt cacataggcc aaagggcgaa tgacgatgtg ttcgccattg
     2101 tcagacacca atttcggcgg catggatttg agcttgccac cgaagaacat gttcaaaaac
     2161 agcgtttcca aaatgtcgtc gcggtggtgg cccaaggcga ttttgctaca gcccaattct
     2221 ttggccacgc ggtataacac gccgcggcgt aagcggctgc acaaagcaca agtggttttg
     2281 ccttcaggca ccacgcgttt gaccacgctg taagtgtctt cttcaatgat gtggtaggca
     2341 atgcccagct tttccaaata ctcgggcaac acgtgggtag ggaagccagg ttgtttttgg
     2401 tcgagattca cggccacgat gtcaaaatca atcggtgcgg atttttgtaa gcccagcaaa
     2461 atgtcgagca tggcgtaaga atctttgccg ccagagaggc aaaccatgat tttgtcgccg
     2521 tgttctatca tgccaaaatc attgatggca tcgcccaccg catggcgcag gcgtttcatc
     2581 agtttattgg cttcgagttc ggctttgctt ttttcagaca taatggcaac aggcttaata
     2641 gaaagggctg cgattatacg cgaaaaccac gaattttggg tggttttgtt ggctaaggca
     2701 tttattccta tcggaaaatt gcctgtgctg agcgggcgca atgcagccgt atgcctgtag
     2761 tgcaagacag ggcgtcatca ggaaacggta tttcgttgct ttctttattg ttgttcgcac
     2821 aagtctttca ggcgctgcaa agccgctggc catgcgtgat caaaaaattc gatgcaggct
     2881 tcgttattgt ctacttccag cgtgcatgag agtttggtgc cttggctgat gggaatgaag
     2941 cggtaggttt ccaaggctga attccaattg tgtggcgttt gcgggttgtt tttccattga
     3001 tccacatacc gcaattgtat caattcgcct ggttgatttt tgtctacccg tgcgaccatg
     3061 ccccattggg tttgatttgc tccaacaaag gtgaacaaca tttgtgagcc ttctgaccaa
     3121 tcgccatcga agcagcttgc gttgctaaac gagtacgtcc atgtggcata ggcttctttg
     3181 tctaccaaca cattgtgcac ggtgagaggg tcttgatcga tttgtatgct gtagtgcagt
     3241 tgtggcatgg taattcccct ttacaaaccg atgaattgga atgttgcgtg tgagcagatg
     3301 cggcctgtaa gcaagcatta caggccgcat taagggttta gtacaattgt ttgtgttcga
     3361 gttccgaata ctcttggaat aaatccaagc acgatgcgcg ttcacactct tgtaagagct
     3421 tggccttgcc ctcaggcgtg gccacgcggt aaaacacttc atcacgaaag ccgatattgt
     3481 cggtgtcttg gatattgcag ccgtaataca cttgctcgat gttggcccac aaaatcgcgc
     3541 ccaagcacat tgggcaaggc tcggcagtgg tgtaaagcat ggcgccgctc aaatcatggc
     3601 tgttcagatt cttacaggca tcgcgtatgg ccaccacttc gccgtggcaa gtggggtcgc
     3661 gttgcttta
//
LOCUS       DAOMVK010000204         3657 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_7389_length_3657_cov_2.229595, whole genome shotgun sequence.
ACCESSION   DAOMVK010000204 DAOMVK010000000
VERSION     DAOMVK010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7389_length_3657_cov_2.229595"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PK856_07290"
     CDS             complement(<1..191)
                     /locus_tag="PK856_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02208.1"
                     /translation="MKNKVFLVSGLLVLSACTSVQVNAVAENQVNAVHQICIVNNPKV
                     LVKDFVPVVQSRLQHHGIVS"
     gene            complement(349..786)
                     /locus_tag="PK856_07295"
     CDS             complement(349..786)
                     /locus_tag="PK856_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PACE efflux transporter"
                     /protein_id="HRL02209.1"
                     /translation="MQLQGVKRKIIQAISFEVLAVIMVSPFVAMLFGKSLSNAAGLSI
                     LLSFLALFWNFAFNTMFEAWEIRQVNRQRTLKRRIIHAVGFEAGFAILVVPVVAYWLN
                     ITWQAALVTDLGLMLFFMFYQFGFQWAFDKIFGVPRSAMANIS"
     gene            890..1777
                     /locus_tag="PK856_07300"
     CDS             890..1777
                     /locus_tag="PK856_07300"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRL02210.1"
                     /translation="MFKDLPLNALRAFEATARLGEIRLAAAELFVTPAAVSQQLKKLE
                     SQLNLNLWDRHGNKITLTAAGEQLYSSCHQCFSALKQNIHTLQPHANTNSVSLQLPDS
                     LATLWLIPRLGDFYQQHPDIDVKISTSDDWQHIEPDFNIDVYIRCQPEHSDFDSAFEH
                     DKMFAEYFGVYASPHFNLNNKINLIAIQSDEVVYDSINWARWLEQYPHPSLKTADLRY
                     FDSEHDMIQAAISGYGLVLASNVLVSKSLEQGVLKALFPQQILPWHELYYASYRTGRQ
                     RHSAVATLLSWLGQQSALS"
     gene            complement(1969..2721)
                     /gene="gloB"
                     /locus_tag="PK856_07305"
     CDS             complement(1969..2721)
                     /gene="gloB"
                     /locus_tag="PK856_07305"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HRL02211.1"
                     /translation="MKIVPISAFNDNYIWLIHDEESAVCVDPGDAAPVLAALEQKQLD
                     LDAIILTHHHNDHIGGVADLQAAYPDVAVYGPDDIACVNQALKEGQELNLLGQVWRVW
                     FVPGHTHNHLAYILRIEGKNRLHVFCGDTLFSAGCGRVFSGTVAQLEQSLQRFAGLPK
                     DTLFYPAHEYTASNLRFALSVEPGNEAANKALEAAEKTPTLPVSLSHEKSINPFLRTD
                     SAEIIASVSAKNLPHATASELFASLRELKNQF"
     gene            complement(2734..>3657)
                     /locus_tag="PK856_07310"
     CDS             complement(2734..>3657)
                     /locus_tag="PK856_07310"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HRL02212.1"
                     /translation="VIALISGGASALLSLPCADISLEDKRAVNKALLHSGAPIEHMNA
                     VRKHLSAIKGGRLAQAAAPAQVLTLAISDVVGDDLSVIGSGPTVSDASTFADVKRILQ
                     QYQIELPAAVAAYLQAASDADETPKSLPNSQAHLVVTPNQALMHTEAWAKAQGLNVLY
                     LGDQISGESKDVAQVMAGIAKHIAAQNLPIAKPALLLSGGETTVTVKNPLGKGGRNTA
                     FLLALATALQGQEGIYALAADTDGIDGSEDNAGAWFAPETWQQGLALGLDAEALLQQD
                     LAYRYFEALNQLLVTGPSRTNINDVRALLIV"
BASE COUNT         1013 a          938 c          772 g          934 t
ORIGIN      
        1 gaaacaatgc catggtgctg caaacgactc tgcaccacgg gcacaaaatc cttaaccaac
       61 accttcggat tgttcacaat acaaatttga tggacagcat tgacttggtt ttcagccaca
      121 gcattcactt gtacactggt acaagcggac aaaaccagca aacctgaaac caaaaacact
      181 ttatttttca ttacacactt ctattctttt aacattatta aagagtgcgc aatttatcac
      241 atatgcataa aaaagaaacc agtatttatc atgactggtt tttttcactc actgcacagt
      301 ttttaatttt cagatatcag caatcttaaa attcactgat gtttcaaatt aggaaatatt
      361 ggccattgcc gaacgtggca ccccaaagat tttgtcaaat gcccattgaa aaccgaattg
      421 gtaaaacatg aaaaacagca tcaagcccaa atctgtcacc agtgcggctt gccaagtgat
      481 gttcaaccaa tacgccacca cgggcaccac caaaattgca aaacctgcct caaagcctac
      541 cgcgtgtatg atgcggcgct taagcgtgcg ttgacggttt acttgacgta tttcccatgc
      601 ttcaaacatg gtgttgaaag caaaattcca aaacaaggcc aaaaacgaca gcaaaatgga
      661 caagccagct gcattagata aactttttcc aaacaacatg gccacaaacg gagacaccat
      721 aatcaccgcc aacacttcaa aactgatggc ctgaatgatt ttacgtttta caccctgcaa
      781 ctgcatgacc cacctcttga taaaccgata ttttgaggct gaatgataaa aatgtttgcg
      841 ctatactgca aacgagtttt cttccataca ctttagaaaa actaatgcca tgttcaaaga
      901 cctgcctcta aatgccttgc gtgcgtttga agccactgct cgattgggtg aaatcaggct
      961 ggccgctgcc gaactgtttg ttactcctgc ggctgtgtcc cagcaattaa aaaagttgga
     1021 gagtcagtta aacctcaacc tatgggacag acatggcaat aaaatcaccc ttacagcggc
     1081 aggtgagcaa ctgtacagca gttgtcatca atgcttctct gccctgaaac aaaacatcca
     1141 taccctacaa cctcatgcaa ataccaacag tgtcagccta caactgcccg actcactcgc
     1201 cactttgtgg ctgataccca gactgggcga tttttatcaa caacatccag acattgatgt
     1261 gaaaatttcg accagcgatg attggcaaca catcgagcct gatttcaaca ttgatgtgta
     1321 catacgttgc cagcctgagc acagcgattt tgattcggct tttgaacacg acaaaatgtt
     1381 tgccgagtat tttggtgtgt atgcctcgcc acacttcaat ctgaacaata aaatcaattt
     1441 gatagccatc caatctgacg aggtggttta cgacagcatc aattgggctc gctggctaga
     1501 gcaataccca catccaagtt tgaaaacagc agatttgcgc tatttcgaca gcgagcatga
     1561 catgatacag gccgccattt caggctatgg tttggttttg gcaagcaatg tgttggtgtc
     1621 aaaaagtttg gagcaaggtg tattgaaagc gctgtttcca cagcagattc tgccttggca
     1681 cgaactgtat tacgcctcgt accgaactgg cagacaacgg cactcagccg ttgccacttt
     1741 gctgtcttgg ctaggacaac aatctgcgtt atcgtaaaat ctcttgccac tttgatgatt
     1801 gagccatgtg aatacaatta aaatactcaa cgcgttatgt ttatttatgc atggataaaa
     1861 tatgattccg cacattaatt cgctcatatt gcttaatacc gccatttcac aagcacaaaa
     1921 aagcggcctg taaaccatta caggcctctt gttccatctc gtgttgcttt aaaactggtt
     1981 tttcagttca cgcaaagagg caaataactc gctcgcagtt gcgtgtggca agttttttgc
     2041 agacacactg gcaatgattt cagccgaatc ggtgcgcaaa aatggattaa ttgatttttc
     2101 atggcttaag ctcactggta aagtgggcgt tttttctgcc gcttccaagg ccttgtttgc
     2161 ggcctcattg ccaggctcaa cactcaaagc aaagcgcaaa ttgctggcgg tgtattcgtg
     2221 agcagggtaa aacaaagtgt ctttgggcaa gcccgcaaag cgctgtaagc tttgctccaa
     2281 ttgcgccacg gtgccagaaa acacccgacc acaccctgcg ctgaacaagg tatcgccgca
     2341 aaatacatgt aagcggtttt ttccttctat gcgaagaatg taagccaagt gattgtgggt
     2401 atgacctggc acaaaccaca cacgccacac ctgtcccaac aaattcaact cttggccttc
     2461 tttcaaggcc tgattcacac aagcgatgtc atctgggcca tacacagcca catcaggata
     2521 agcggcctgt aaatcggcga ctccaccaat gtggtcattg tggtggtggg tcaaaatgat
     2581 ggcatccaaa tccagttgct tttgctccaa ggccgccagc actggcgcag catcgcctgg
     2641 atccacacac acagccgatt cttcatcgtg tatcaaccaa atataattgt cattaaatgc
     2701 cgaaatgggc actattttca ttgtgttcac cttttataca attaacagcg cacgcacatc
     2761 gttgatgttg gtgcgactcg gccctgtcac cagcaactgg ttcaaagcct caaaataccg
     2821 atatgccaaa tcctgttgca acaaggcttc ggcatccaag cccaatgcta gtccttgctg
     2881 ccaggtttca ggcgcaaacc aagcgccggc attgtcttca ctgccgtcta tgccatcggt
     2941 gtcggcagcc aaggcataaa tgccctcttg accttgcaat gctgtcgcca aagccagcaa
     3001 aaatgcggtg ttgcgtccgc ctttgcccaa tggatttttc actgtcaccg tggtttcacc
     3061 acctgataaa agcaaagctg gtttggcgat gggcaggttt tgcgccgcga tgtgtttggc
     3121 tatgcctgcc atcacttgcg ccacgtcttt ggattcacca ctgatttggt cacccaaata
     3181 caacacgttc aagccttgcg ctttggccca agcctcggta tgcatcagtg cttgattcgg
     3241 agttaccacc aaatgagctt ggctattggg caaggattta ggcgtctcat ccgcatcaga
     3301 tgcggcctgt aaatatgccg ccacagcagc gggcaattca atttggtatt gctggaggat
     3361 gcgtttcaca tcggcaaacg tgctggcatc ggacacggta ggacctgaac caatcacact
     3421 caaatcgtcg cccaccacat ccgaaattgc caaggtcaaa acttgtgcag gtgcggccgc
     3481 ttgcgccaat cgcccgcctt tgatggcaga caggtgtttg cgcaccgcat tcatgtgttc
     3541 gatgggtgcg ccgctgtgca gcaaggcttt gttgactgcg cgcttgtctt ccaacgatat
     3601 atcggcacat ggcaaagaca acaaagccga agcaccgccc gaaatcaagg caatcac
//
LOCUS       DAOMVK010000205         3652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_7402_length_3652_cov_2.381985, whole genome shotgun sequence.
ACCESSION   DAOMVK010000205 DAOMVK010000000
VERSION     DAOMVK010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7402_length_3652_cov_2.381985"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="PK856_07315"
     CDS             <1..865
                     /locus_tag="PK856_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRL02213.1"
                     /translation="GFEVTYLDVQENGLIDLNVLKAALRDDTILVSVMWVNNEIGVIQ
                     DIDAIGEICRERGIVFHVDAAQAAGKVEIDVTKTKVDLLSLSGHKIYGPKGIGALYVR
                     RKPRIRLEAQMHGGGHERGFRSGTLASHQIVGMGEAFRIAKEEMAAENERLLGLRNRF
                     LELIDGIEEVYINGDMEHRYVGNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLEP
                     SYVLRALGRNDELAHSSLRITFGRFTTMEEVEYAANLLKSKIGKLRDLSPLWEMHQEG
                     IDLSTVQWAEH"
     gene            913..1299
                     /gene="iscU"
                     /locus_tag="PK856_07320"
     CDS             913..1299
                     /gene="iscU"
                     /locus_tag="PK856_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HRL02214.1"
                     /translation="MAYSDKVIDHYENPRNVGSFDKGDDSVGTGMVGAPACGDVMKLQ
                     IKVSEAGVIEDAKFKTYGCGSAIASSSLITEWVKGKTLDEALSIKNSEIAEELALPPV
                     KIHCSILAEDAIKAAVSDYKTKHGDK"
     gene            1380..1700
                     /gene="iscA"
                     /locus_tag="PK856_07325"
     CDS             1380..1700
                     /gene="iscA"
                     /locus_tag="PK856_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HRL02215.1"
                     /translation="MITITERAATHIQNFLNNRGKGQGIRLGVRTSGCSGMAYTLEFA
                     DGALDEDMVFEGFGVKVFVDPKSHVYLDGTELDYTKEGLQEGFKFINPNVKDECGCGE
                     SFHV"
     gene            1729..2229
                     /gene="hscB"
                     /locus_tag="PK856_07330"
     CDS             1729..2229
                     /gene="hscB"
                     /locus_tag="PK856_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HRL02216.1"
                     /translation="MAIDYYKIFALPKSYALDAAALEQNYRRLSTQHHPDKTAHLSAF
                     EQKQAVMLSATINEAYRILKSPLDRAAYLLQEQGIHADAPENTRFEAAFLMQQMQWRE
                     DLDDAGHDENALLALNRQIVAARDALQAALNQDFADEQWETAANHVREGRFIHKLLQQ
                     IKQALP"
     gene            2250..>3652
                     /gene="hscA"
                     /locus_tag="PK856_07335"
     CDS             2250..>3652
                     /gene="hscA"
                     /locus_tag="PK856_07335"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HRL02217.1"
                     /translation="MALLQIAEPGMSAAPHQHRLAVGIDLGTTNSLVATVQSGSARCL
                     PDEQGRVTLPSVVRYGQEVEVGFDALKAQKIDPLNTISSAKRLIGRQVGDLNRSQWSY
                     RFTDNDKIIEMQTRMGLKTPIEVSAEILKALKIRAENSLGGSLTGAVITVPAYFDDAQ
                     RQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNRAEGTFVVFDLGGGTFDVSILRLEQ
                     GLFQVLATGGNSALGGDDFDHRLYCWLLEHEKLSALSEQDTQLLLSLIRAAKQELTNN
                     ASTTLRAVLSNGREVESNITREHFQQLTQHLVMKTIEPVKQAMRDADVSKADIGGVIM
                     VGGATRMPHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKADDEWLLLDVT
                     PLSLGLETYGGLVEKVIPRNSTIPTAMAQEFTTFKDGQTAMAIHVVQGERELVSDCRS
                     LARFTLRGIPPMVAGAAR"
BASE COUNT          982 a          905 c          927 g          838 t
ORIGIN      
        1 aggctttgaa gtgacttact tggatgtgca agaaaacggt ttgattgatt taaacgtatt
       61 gaaagcggcc ttgcgtgacg acaccatttt ggtgtctgtg atgtgggtta acaatgaaat
      121 tggtgtgatt caagacatcg atgccatcgg cgaaatctgc cgcgagcgtg gcattgtatt
      181 ccatgtagac gcagcccaag cggccggtaa agtggaaatt gatgtcacca aaaccaaagt
      241 agacttgctc agcttgtcag gtcacaaaat ttacggccct aaaggcatcg gtgctttgta
      301 tgtacgccgc aaaccacgca tccgcttaga agcacaaatg cacggtggtg gccatgagcg
      361 tggtttccgt agtggcacat tggcctcaca ccaaattgtg ggcatgggag aggccttccg
      421 cattgccaag gaagaaatgg ctgccgaaaa cgaacgctta ttgggcttgc gcaatcgctt
      481 cttagaattg attgatggca ttgaagaagt gtacatcaat ggcgatatgg agcaccgcta
      541 cgtgggcaat ttgaacgtca gcttcaactt cgttgagggc gagagcctga tcatggcagt
      601 gaaagaattg gctgtttcca gtggatcagc ctgtacttca gccagcttgg aaccttctta
      661 cgtgttgcgc gcattgggcc gcaacgatga attggctcac agctcattgc gcatcacttt
      721 tggccgcttc accaccatgg aagaagtgga atacgctgcc aatttgttga aatcaaaaat
      781 cggcaaattg cgcgacttat cgccattgtg ggaaatgcac caagaaggca tagacttgtc
      841 taccgtacaa tgggcagaac actaatttaa ccaatatttt aagcggttca ggccgcttct
      901 gaggagcaca acatggcata cagcgacaaa gtaatcgatc attacgaaaa tccccgcaac
      961 gtgggttctt ttgacaaagg tgacgacagt gtgggcactg gcatggtcgg cgcacccgct
     1021 tgtggcgacg tgatgaaatt gcaaatcaaa gtgagcgaag caggcgtaat tgaagatgcc
     1081 aaattcaaaa cttatggctg cggctctgcc atcgcctcat cgtctttgat taccgaatgg
     1141 gtaaaaggca aaaccttgga cgaagccttg tccatcaaaa acagcgaaat cgctgaagaa
     1201 ttggctttgc cacctgtgaa aattcactgc tctattttgg ctgaagatgc catcaaagca
     1261 gctgtgagcg attacaaaac caaacacggc gacaaataat ccgccttaca ggccgcttgc
     1321 agcgatatta agcggcctgt aaacccctat cctgcttcaa gcaacaccaa ggatgcaaca
     1381 tgattaccat taccgaacgt gcggcaacac acattcaaaa cttcttaaac aaccgtggca
     1441 aaggccaagg catacgcttg ggcgtgcgca ccagcggctg ctcgggcatg gcttatacct
     1501 tggaattcgc cgatggtgct ttggatgaag acatggtatt tgaaggcttt ggcgtgaagg
     1561 tatttgtaga cccaaaaagc catgtgtatt tagacggcac cgagcttgat tacaccaaag
     1621 aaggcttgca agaaggtttc aaattcatca atccgaatgt caaagacgag tgtggttgtg
     1681 gtgaaagctt tcacgtttaa gcatcgctaa ggtttcagga cgcctgttat ggccatagat
     1741 tattataaaa tttttgcatt acccaaatct tatgcacttg atgccgcggc tttagaacaa
     1801 aactaccgcc gcttatcaac ccaacaccat ccagacaaaa cagcacattt gtcggctttt
     1861 gaacaaaagc aagcagtgat gctgtcggcc accatcaacg aagcctaccg catcctcaaa
     1921 tcgccgttag accgtgccgc ttacctgctg caagagcaag gcatacacgc cgatgcacct
     1981 gaaaacaccc gttttgaagc ggcgttcttg atgcaacaaa tgcagtggcg tgaggattta
     2041 gacgatgctg gccacgatga aaatgccttg ctcgcgctca atcgccaaat tgtggctgcg
     2101 cgcgatgcct tacaggccgc tttaaaccaa gactttgctg atgaacaatg ggaaacagcc
     2161 gccaatcacg tgcgtgaagg ccgtttcatc cacaaattat tgcaacaaat caagcaagcc
     2221 ttaccttaat acaaagacag aacacaccca tggctttatt acaaatcgcc gaaccaggca
     2281 tgtcagcagc cccacaccaa caccgcttgg ctgtgggcat agacttaggc accaccaaca
     2341 gtttggtcgc caccgtacaa agcggcagcg cccgttgttt gcccgatgaa caaggtcgcg
     2401 tgactttgcc ctcagtggtg cgttatggcc aagaagtaga agtgggcttt gatgctttaa
     2461 aagcccaaaa aatcgacccg ctcaacacca tcagctcggc caagcgttta atcggtcgtc
     2521 aagtcggcga tttgaatcgc agccaatggt cttaccgctt caccgacaac gataaaatca
     2581 tcgaaatgca aacgcgcatg ggtttgaaaa caccgataga agtgtctgca gaaatcctca
     2641 aagccttaaa aattcgtgct gaaaacagct tgggcggcag cctaacgggt gcggtgatta
     2701 ccgtgcctgc ttattttgac gatgcccagc gccaagccac caaagatgcc gcacgtttgg
     2761 caggcttaaa cgtgttgcgt ttgctcaacg aacctaccgc cgccgccatt gcctatggtt
     2821 tggacaaccg tgctgaaggc acgtttgtgg tgtttgactt gggtggtggc acgtttgatg
     2881 tatcgatact gcgtttggaa caaggtttgt tccaagtgtt ggccacaggt ggcaacagcg
     2941 ctttgggtgg cgacgatttc gaccaccgct tgtattgctg gttgcttgag catgaaaaac
     3001 tgtcggcctt gagcgaacaa gacacgcaat tgttgttaag cctgattcgc gccgccaaac
     3061 aagagctgac caataatgcc agcacgactt tgcgtgcggt attgtcgaat ggacgcgaag
     3121 tggaatccaa catcacccgc gagcatttcc aacaattgac gcaacacttg gtgatgaaaa
     3181 ccatagagcc cgtgaagcaa gcgatgcgcg atgcggatgt cagcaaagcc gatattggcg
     3241 gtgtcatcat ggtgggtggc gccacacgca tgccgcatgt gcagcaagca gtggccactt
     3301 tctttggcca aaccccgctc aataacctga atccagacga agtggtggct ctgggcgctg
     3361 ccatacaagc aaatgtgttg gcaggcaata aagccgatga tgaatggctg ctgcttgatg
     3421 taacgccttt gtctttggga ctggaaacct atggcggctt ggtggaaaaa gtcattccac
     3481 gcaattcgac cattcccact gcaatggcgc aagaattcac cacctttaaa gacggccaaa
     3541 ccgcgatggc gatccatgtg gtgcaaggcg agcgcgaatt ggtgagcgat tgccgctcac
     3601 tcgcccgctt taccctacgc ggcattccgc ccatggtggc aggcgcagcg cg
//
LOCUS       DAOMVK010000206         3624 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_7476_length_3624_cov_2.986271, whole genome shotgun sequence.
ACCESSION   DAOMVK010000206 DAOMVK010000000
VERSION     DAOMVK010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7476_length_3624_cov_2.986271"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PK856_07340"
     CDS             complement(<1..439)
                     /locus_tag="PK856_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HRL02218.1"
                     /translation="MGSAVSGFQIGDEVYYAGVVNRAGCNAEYQTVDARIIAKKPQSL
                     DFAQAAALPLTSLTAYEGLFDRLQVNEHSNMHNRVLLVIGGSGGVGSQIIQLAKQLSD
                     LTVVATASRPESQVWCEELGADFVINHRHDLAEEWAKTGLPAPS"
     gene            719..1087
                     /locus_tag="PK856_07345"
     CDS             719..1087
                     /locus_tag="PK856_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRL02219.1"
                     /translation="MSNSKHTHFECAVGCSVEATLELIGGKWKGVILYHLLQESVLRF
                     SQLQKLFPSCTQRTLTNQLRALEADGFIKRVVYAQVPPKVEYSLTERGLTLAPVLLAL
                     KAWGDVELGKTDVAMATSGL"
     gene            complement(1128..2387)
                     /locus_tag="PK856_07350"
     CDS             complement(1128..2387)
                     /locus_tag="PK856_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL02220.1"
                     /translation="MSDSVYKHQGFVAFVSARLMAVLATQIQSVVVAWQVYDLTRDPM
                     ALALVGLAQFIPMLLLVLPAGDWIDRFNRKWVLGGSWLVGCACSLMLWWLAGHGESGI
                     DGIYAVLLVYGCSRAFSAPAMQSLLPQVVAREQLPAAIAANSMLMRGAAIGGPLIGGF
                     LYAYGGGSATFIVCALCFGLASSLLMRVPLLDPEGVKAAMRLARQAKSSMVERVQEGM
                     RFIWRHPVVLGSISLDLFAVLLGGVIALLPIYAQEVLHVNASGLGILRSSMAVGEVLC
                     GLYLSTHPFNKNIGRTLFIAVTVFGVANLVFAFSHLFLLSCAALFVAGAADMVSVYIR
                     SSMVQLSTPDNMRGRVNSVNSLFISSSNELGEFRAGSSAAAFGVVPAAVMGGVCTLLV
                     VGLWSKWFPSLRQANQFSDVEYRDKQS"
     gene            complement(2456..3238)
                     /gene="lpxA"
                     /locus_tag="PK856_07355"
     CDS             complement(2456..3238)
                     /gene="lpxA"
                     /locus_tag="PK856_07355"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HRL02221.1"
                     /translation="MAATNIHPQAIVDPKAQLAEGVSVGAFSIIGPNVSIDAGTEVGP
                     HVVIDGHTSIGKNNKIFQFASLGAQPQDKKYAGEPTKLIIGDNNTIREFTTFNTGTVT
                     GIGETRLGNDNWIMAYVHLAHDCVVGNHTIFANNASLAGHVTIGDWVILGGYSLVHQF
                     CILGDYSMTAFAAGVHKDVPPYVMAAGYRAEPKGLNTEGMRRRGMSPEQIERVQHVYK
                     VLYRQGLSYNDAKAQIIELAQSQDEVKVFAEFFAQSTRGVIR"
     gene            complement(3242..>3624)
                     /gene="fabZ"
                     /locus_tag="PK856_07360"
     CDS             complement(3242..>3624)
                     /gene="fabZ"
                     /locus_tag="PK856_07360"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HRL02222.1"
                     /translation="LDRVTAFEANQTLTAIKNVTMNEPFFQGHFPGFPVMPGVLITEA
                     LAQACGTLAILSEGGRNDKEIYFFAGIDNARFKRQVIPGDQLVFEVELLQSKRGIGKF
                     KAVAKVDGQVAAEAEIMCAKKVVG"
BASE COUNT          961 a         1040 c          836 g          787 t
ORIGIN      
        1 agcttggcgc aggcaaacct gttttcgccc attcttctgc caaatcatgg cggtgattga
       61 tgacaaaatc cgcacccaat tcctcacacc aaacttggct ttcaggccga gaggccgtcg
      121 ccaccaccgt taaatccgac aattgtttcg ccaactgaat aatttgcgag cccaccccgc
      181 ccgagccgcc gattaccaac aacacacgat tgtgcatgtt gctgtgctca ttcacttgca
      241 agcggtcaaa caaaccttcg taagcggtta agctggtcaa aggcaaggca gcggcttggg
      301 cgaaatccaa gctttgcggc tttttggcga tgatgcgggc gtcaacggtt tggtattcgg
      361 cattgcagcc cgcgcggtta accacgcctg cataatatac ttcgtcgccg atttgaaaac
      421 cactgaccgc gctgcccact tcggcaacca cccctgccgg attttgatgt caacagggtt
      481 gacagcgatg gcttcaattt tcaccaacac ctcatgcgcg gcaacactgg gcatgggcgc
      541 ttcaaatgcc aacaaagact gcgcatcggt gatgggcaaa gacgtttcaa aacctatggc
      601 ttgcatggca atactccttt gtgaacagtg ctgctatcat agcctgaacc ttgcctgagc
      661 gtaagaacgc acttaatgga cagccagtgt cttaaaagac accataaaga gaacacacat
      721 gagcaacagc aaacacaccc attttgaatg cgccgttggt tgcagcgtgg aagcgacttt
      781 ggaattgatt ggtggcaaat ggaaaggcgt gattttgtac catttgctgc aagaatcggt
      841 gttgcgcttc agccaattgc aaaaactgtt tcccagctgc acccaacgca cgctcaccaa
      901 ccaattgcgc gccttagaag ccgatggctt catcaaacgc gtggtctacg cacaagtgcc
      961 gccaaaagtg gaatactcgc tcaccgaacg tgggctgaca cttgcgcccg tcttattggc
     1021 tttgaaggct tggggcgatg tggaattggg caaaaccgat gtggccatgg cgacaagcgg
     1081 cctgtaaaca tttacaggcc gcttatgagc cactccatca accacagtta actttgtttg
     1141 tcgcggtatt ccacatctga aaactgattg gcttggcgca ggcttggaaa ccatttcgac
     1201 cacaaaccca ccaccaacaa ggtacataca ccacccatca ccgccgcagg caccacgcca
     1261 aacgccgccg cgctgctgcc cgcacgaaat tcacccaatt cattcgatga gctgatgaac
     1321 aagctgttca ccgaattcac acggccacgc atattgtcgg gtgtggacaa ttgcaccata
     1381 gaactgcgga tgtaaacgct gaccatgtct gccgcacctg ccacaaacaa agccgcgcac
     1441 gacaacaaaa acagatgtga aaaggcaaac accaaattcg ccaccccaaa cacggttacc
     1501 gcaataaaca aggtacggcc aatgttttta ttaaagggat gggtacttaa atacaggccg
     1561 cacaatacct cacccaccgc catggagctg cgcaaaatgc ccaagcccga agcattcaca
     1621 tgcaaaacct cttgcgcgta tatcggcaac aaagcgatga cgccaccaag caacaccgca
     1681 aacaaatcca atgaaatcga gcccaatacc acagggtggc gccaaataaa gcgcatgcct
     1741 tcttgcacgc gttccaccat agaagatttg gcctgtctgg ccaagcgcat cgcggctttt
     1801 actccttctg ggtctaacaa aggcacgcgc atcaacaaac tgctcgccaa accaaaacac
     1861 agcgcacaca cgataaacgt ggcactgccg ccaccgtaag catacaaaaa accaccaatc
     1921 aacgggccgc ctatggccgc accgcgcatc aacatagagt tcgcggcgat ggcagcaggc
     1981 aattgctcac gcgccaccac ttgtggcaac aaggattgca tggctggcgc cgaaaacgca
     2041 cggctgcaac cataaaccaa taacaccgca taaataccat ctatgccaga ttcgccatgc
     2101 cctgccagcc accacagcat caatgaacat gcacagccca ccagccaact gccacccaat
     2161 acccatttac ggttgaaacg gtctatccag tcgcccgcag gcaacaccaa taacaacatc
     2221 ggaatgaatt gtgccaagcc caccaaagcc aaggccatgg ggtcacgggt taaatcatag
     2281 acttgccacg ccaccaccac cgattgaatt tgcgtggcca ataccgccat caaacgtgcg
     2341 ctgacaaagg ccacaaagcc ttggtgtttg tataccgaat cactcatctt gttctttttc
     2401 tgaataaaca aaagcgtcct gtaacttaca ggacgcttgt caaaccatca agcctttaac
     2461 gaatcacgcc acgcgtagac tgggcgaaga attctgcaaa aaccttcacc tcgtcttggc
     2521 tttgcgccaa ctcgatgatt tgcgctttgg catcgttata gcttaaacct tgacggtaca
     2581 acactttgta cacgtgttgc acccgctcaa tttgttctgg gctcatgcct cggcggcgca
     2641 tgccttcggt gttcaagcct ttgggctcag cgcgatagcc agcggccatc acatacggtg
     2701 gcacatcttt gtgcacgcct gcggcaaatg ctgtcatgct gtagtcgccc aagatacaga
     2761 attggtgtac caaggagtag ccgcccaaaa tcacccaatc accgatggtg acatggccgg
     2821 ccaatgaggc attgttggca aaaatggtgt gattacccac cacgcaatcg tgcgccaaat
     2881 gcacataagc catgatccag ttgtcattgc ccaagcgggt ttcgccaatg cctgtgaccg
     2941 tgcccgtgtt aaacgtggtg aattcacgga tggtgttgtt gtcgccgatg atgagcttag
     3001 tgggttcgcc cgcgtatttt ttgtcttgcg gctgcgcacc caatgaggca aattggaaaa
     3061 tcttgttgtt tttgcctatg ctggtgtggc catcaatgac cacatgcggg ccaacctctg
     3121 tgcccgcatc aatgctgaca ttcgggccga tgatggaaaa tgcacccaca ctcacgcctt
     3181 cggcaagctg agctttcggg tcaacaatgg cttggggatg gatgttggta gcagccatgg
     3241 cttatcccac tacttttttg gcacacatga tttcagcctc agccgccact tggccatcta
     3301 ctttggccac ggctttgaat ttgcctatgc cgcgtttgct ttgcaacaac tcgacttcaa
     3361 acaccaattg gtcacctggg atgacttgac gcttgaaacg cgcgttatca atgcccgcaa
     3421 agaaatagat ttctttgtcg ttgcggccac cttcagacaa aattgccaaa gtgccacagg
     3481 cttgtgccaa ggcttcagta atcaacacgc ctggcatcac aggaaaacct gggaaatggc
     3541 cttggaaaaa aggctcattc atggtgacgt ttttgatggc ggttaaggtt tgattggctt
     3601 caaaagcagt gacgcgatca agca
//
LOCUS       DAOMVK010000207         3622 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_7483_length_3622_cov_3.449117, whole genome shotgun sequence.
ACCESSION   DAOMVK010000207 DAOMVK010000000
VERSION     DAOMVK010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7483_length_3622_cov_3.449117"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PK856_07365"
     CDS             complement(<1..204)
                     /locus_tag="PK856_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02223.1"
                     /translation="MNHSSKCALMFSTVLLLASTSSLANTQGYLNAKQKISNEIVSCL
                     KNGGMEGKAALNECYYDAAMSFSK"
     gene            502..915
                     /locus_tag="PK856_07370"
     CDS             502..915
                     /locus_tag="PK856_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02224.1"
                     /translation="MMAKSKFDGIVDKMVAGDFNEASVKLTEDELDFLQQHPLLLAKI
                     SDGAFIKKKYIYRLFGISVGMMAVAKVLEYGEWLAQYKIINNLLTEVQFSISMELLGA
                     TVIAYFLEITLERRVERNKRLVAQIMARGHVNESN"
     gene            complement(1032..1634)
                     /locus_tag="PK856_07375"
     CDS             complement(1032..1634)
                     /locus_tag="PK856_07375"
                     /inference="COORDINATES: protein motif:HMM:NF025501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4303 domain-containing protein"
                     /protein_id="HRL02225.1"
                     /translation="MNQTEFHPELDTALRTYLQAAFDWLREHHQHEHLYAVGLGMVED
                     ICGFFIVGNTLERLADLSHGLKDTSEANKTGYFWSISEWKSEEFLGLRNLVGQVVADI
                     AHPLSIDDDAYDAFRLVYQDHILNVLADMRGQGLLQNAQSEELWVWVQYADAYDEDFD
                     DVSFARLNPPELSHLFSLRYEPNKHNLTHVLHERLQTLPH"
     gene            2108..2785
                     /locus_tag="PK856_07380"
     CDS             2108..2785
                     /locus_tag="PK856_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HRL02226.1"
                     /translation="MTTPANFNGQAPVIDVNDSVMLLIDHQSGLFQTVGDMPMTELRA
                     RAGALAKIATLAKMPVITTASVPQGPNGPLIPEIHQNAPHAQYVARKGEINAWHNEDF
                     VKAVKATGRKTLIIAGTITSVCMAFPSISAVAEGYKVFAVIDASGTYSKMAQEITLAR
                     MVQAGVVPMDTAAVASEIQGTWNREDAHEWAQTYTQIFPAYQLLMESYAKAQDVLKNN
                     EVLDSNR"
     gene            complement(2886..>3622)
                     /locus_tag="PK856_07385"
     CDS             complement(2886..>3622)
                     /locus_tag="PK856_07385"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRL02227.1"
                     /translation="IGHRYYQHCQAMLIEAEAAQAAIESAHFEPTGTIRVSCPVTLLQ
                     IHIAPLIFNFMQQYPKITVELEASNRAVDVIGEGFDIAIRARTRLESEDLVAKILSNR
                     HKVLVANTEWLQQRPALTHPQELVGLPSLGLGKPNQTFMWSFQHPNHGTWEQIHFPRL
                     ITTEMTTLLQAAVNGLGLVELPHLVVEKHLATQELTMVLPEWLLPIDIIHAVYPSRRG
                     QLLAVKTFLNFLETFYKQDQESTPSQ"
BASE COUNT          982 a          770 c          926 g          944 t
ORIGIN      
        1 tttgctgaag ctcattgctg cgtcgtaata acattcattg agtgcagctt tgccttccat
       61 gccaccattt tttaaacaac tgacgatttc attggaaatt ttttgcttgg cattcaaata
      121 accttgggtg ttggcaaggc tgctggttga ggcgaggagc aatacagtgg agaacattaa
      181 tgcacatttt gatgaatgat tcatggtttt ccctttaaca atgtccaaca gacaacagtc
      241 tattgatagc aaatactcag caagttttag gccatgtgca tcatcacatc atcaatactc
      301 atgagcggcc tgtaagctta aatcaaggtt tacaggccgc ttgtttgcat gccatcagac
      361 aagcggatgg agtgaattaa ggtgagtgta tgtgaatatg ggatggggca tgggctggtg
      421 taactgcgca caatcagtct ctatggtgtg atggcaagac gagatgctac actgaagcga
      481 ttgaattcta tcggaattag catgatggcc aagagcaaat ttgatggcat agtcgacaaa
      541 atggtggctg gagattttaa cgaagcaagc gtgaaactca ccgaagatga gctggatttt
      601 ttgcagcaac atccgttgct gctggcgaaa atttcggatg gggcgtttat caagaaaaaa
      661 tatatttacc gtttgtttgg catcagcgtg gggatgatgg ctgttgccaa ggtgttggaa
      721 tacggtgagt ggctggcaca gtacaaaatc atcaacaatt tgctgaccga agtgcagttt
      781 tccatttcca tggagttgct gggtgccacc gtcatcgcct attttctgga aatcaccttg
      841 gagcggaggg tagaaagaaa caagcgtttg gtggcacaaa tcatggccag aggccatgta
      901 aacgaatcca attgagtgct tggttttact cgaatgggca tagcttgggt gatgacagtg
      961 tagaccttga tagcagcggc ctgtaaacct tgttttgggt ttacaggccg cttctttgtg
     1021 ggtttgattc attaatgcgg caatgtctgc aagcgttcat gcagtacatg ggtgaggttg
     1081 tgcttatttg gctcatagcg cagagaaaat aaatgactga gctcgggcgg attaagacgg
     1141 gcgaagctga catcgtcaaa atcttcatca taggcatcgg cgtattgcac ccacacccac
     1201 aattcctcgc tttgggcatt ttgcagcaag ccttggccac gcatatcggc caacacattc
     1261 aagatgtggt cttggtaaac caagcgaaag gcatcgtaag catcgtcgtc aatgcttaaa
     1321 ggatgggcaa tgtctgcaac gacttgtcca accaaattgc gtaatcccaa aaattcttca
     1381 cttttccatt cagagatgct ccagaaataa cccgttttgt tagcttcgga ggtgtctttg
     1441 agaccatgag acaaatccgc caagcgttcc aatgtgttgc ctacgatgaa gaatccgcag
     1501 atgtcttcca ccatgcccaa acccacggca tacaaatgct catgttggtg gtgttcgcgc
     1561 aaccaatcaa aagcggcctg taaataagtg cgtaaggcgg tatccagttc ggggtgaaac
     1621 tcggtttggt tcatgggaaa tccttgaaaa aaacacaggt aagaggcagc aaaaaggcag
     1681 gtttggagcg tgttgcaagt gccatgtgca tggcatcgtg ctactgaggc attatcaaga
     1741 attcatgttt aactggcaaa tcgactgcac ttaagggaaa aatcaaaaaa gatgctgctg
     1801 gcagtaacac catttgcatg cttggtgatg agtgtgtaag ccaccaaaaa atttgttgat
     1861 agacagaaat ccatgtcgcg caacccgtat ggatgttggg tcgactttta aatggaaaac
     1921 cattgggtgc caccgcaggc gaagcgtcag aaggtaaggt tgttccaaaa ttggaacaat
     1981 cttacctgat tttggtggct actcgagtgg gtttgcttga attaatatgg caacagtttc
     2041 aaaggcatag caaacagtgc taagcatttt ccccattatg atgaacccaa aaataaggca
     2101 aataaccatg actaccccag ctaattttaa cggccaagcc ccagtaattg atgtcaatga
     2161 ttctgtgatg ttgttgattg accaccaaag tggtttgttt caaaccgtag gcgacatgcc
     2221 catgaccgaa ttgcgtgccc gtgcaggtgc tttggcgaag attgccactt tggccaagat
     2281 gccagtcatc accacggctt ctgtgccaca aggcccaaat ggcccattga ttccagaaat
     2341 tcaccaaaat gcgccacatg cccaatacgt ggcccgcaaa ggcgaaatca atgcttggca
     2401 caatgaggat ttcgtgaaag ctgttaaagc cacaggccgc aaaaccttga ttattgcggg
     2461 tacgattacc agcgtgtgca tggcattccc atccatcagt gccgtggcag agggttacaa
     2521 agtgtttgcg gtgattgatg cttcagggac gtattcaaaa atggcgcaag aaatcacttt
     2581 ggcgcgcatg gtgcaagccg gtgtggtgcc tatggacact gcggcggtag cgtctgaaat
     2641 ccaaggcact tggaaccgtg aagatgcgca tgaatgggct caaacctata cccaaatctt
     2701 ccctgcatac caattgttaa tggaaagcta tgccaaagcc caagatgtgt tgaaaaacaa
     2761 tgaagttttg gattcaaacc gttaatcttt gttgattaag gatgacacag ccacctcaca
     2821 ttcatgtggg tggctgtttt gcatggtaca aaccgagtat cacgggtagt ttgaacgatt
     2881 ttttgctatt gacttggagt ggattcttgg tcttgcttat aaaaggtttc caaaaaattc
     2941 aaaaaggttt tgactgccag caattgcccg cggcgacttg gatacactgc atggatgatg
     3001 tctatgggca acagccattc aggtaatacc atggtgagtt cttgggtggc caaatgcttc
     3061 tccaccacca aatgtggcag ctcaaccaaa cccaaaccat tcacagcggc ctgtaacaag
     3121 gtggtcattt cggtggtaat caggcgagga aagtggattt gttcccaggt gccatggttg
     3181 gggtgttgaa acgaccacat gaaggtttga ttgggtttgc ccaagcccaa ggaaggcaag
     3241 cccaccaatt cttgtgggtg tgtcaatgcg ggacgttgtt gcagccactc ggtattggcc
     3301 accaacacct tgtggcggtt ggacaaaatt ttggccacca aatcttctga ttccaaacgt
     3361 gtgcgggccc gaatggcgat gtcaaaaccc tcgccaatca catccacggc ccgattgctg
     3421 gcttcaagct ctacggtgat ttttgggtat tgctgcatga aattgaaaat caaaggggcg
     3481 atgtggattt gcagcaaagt cacagggcag ctcacacgta tggtgcctgt gggctcaaaa
     3541 tgggccgatt caatggcggc ttgagcagct tcggcttcaa tgagcatggc ttggcagtgt
     3601 tgataatagc ggtggccaat tt
//
LOCUS       DAOMVK010000208         3621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_7489_length_3621_cov_2.979529, whole genome shotgun sequence.
ACCESSION   DAOMVK010000208 DAOMVK010000000
VERSION     DAOMVK010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7489_length_3621_cov_2.979529"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="PK856_07390"
     CDS             complement(<1..900)
                     /locus_tag="PK856_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HRL02228.1"
                     /translation="MSSRHIVVLDRGTLMPLPWAFDFEHHLTEHDDTPDNEAINRCRD
                     AEIIITNKVKIDRATIEASPNLKMIAIAATGFEHVDVQAAQERGIIVSNIKAYGNDTV
                     AEHAFMLMMALMRQLPAYQRDVAAGLWQNSPYFCHFGAPIHDVNGKTLLIFGKGSIGS
                     ALAQRAEAFGMKVIYGEHKHASACRDGYVPFAEALARADVISLHCPLNDQTRNLLGEA
                     ELKALKPGCVIINVGRGGLVDETALIAALKYGGLGGAGFDVITNEPPTEGNPLVNTHL
                     PHLIVTPHMAWGSQEAMTRLFHML"
     gene            1014..1268
                     /locus_tag="PK856_07395"
     CDS             1014..1268
                     /locus_tag="PK856_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HRL02229.1"
                     /translation="MMKTLTFAILHFGVAFSVAYALTGSIGISSTVALVEPIVNTVVF
                     FFHEKAWQRIERKRRQKLGLQAALAEKTAHKPWFGCGHSL"
     gene            1332..1655
                     /locus_tag="PK856_07400"
     CDS             1332..1655
                     /locus_tag="PK856_07400"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRL02230.1"
                     /translation="MGLQVEDLVVGDGAEAQSGKEITVHYTGWLTDGTQFDSSIDRKQ
                     PLSIVLGVGQVIKGWDQGIEGMKIGGKRKLTIPPELAYGNHAVGGVIPKNATLVFEVE
                     LLGVA"
     gene            1823..2596
                     /locus_tag="PK856_07405"
     CDS             1823..2596
                     /locus_tag="PK856_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018149794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRL02231.1"
                     /translation="MAVVLFDDGVHKCVAFTDLVAGEGVQANQFAISHQGEGVLLDPG
                     GNLTYKHLLAEISDYFLPSKTRYIFASHPDPDIIASVNGWLLITDAQVLIAQEWERFL
                     PHFCLKGATVGRLKTIPPQGMCLDLNGGELQIIPAHFLHPVGNFHIYDPISKILFSGD
                     VGASLVDAEEAAQTVSDFNHHLHNNGMLGFHQRYMSGNMACRYWVNMVRQLDIEWIVP
                     QHGASFKGKEMVNQFLDWFEGLACGIDLLSQDDFQLLPK"
     gene            2827..3582
                     /locus_tag="PK856_07410"
     CDS             2827..3582
                     /locus_tag="PK856_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRL02232.1"
                     /translation="MAVVLYDDGEHKCVAFSDLVTGEGVQSNQFAVVHKGDGMLLDPG
                     GNLTYKHLLAEISDYFLPSHTRYVFASHADPDIIASINGWLLITDANVLIAKEWERFI
                     PHFCLKGATTGRLISIPAPGMKVELQGAELHLLPAHFLHPVGNFHVYDPISKILFSGD
                     VGASLVDAETAAKPIEDFDHHLHNNGMLGFHKRYMSGNKACRYWVNMVRQLDVEWIVP
                     QHGASFKGKEMVNRFLDWLEDLECGIDLLSQED"
BASE COUNT          893 a          776 c          948 g         1004 t
ORIGIN      
        1 gagcatgtgg aacaagcgcg tcatcgcctc ttggctgccc caagccatgt gtggcgtgac
       61 tatcaaatgt ggcaaatggg tgttgaccaa aggattgcct tcggtcggcg gctcattggt
      121 aatcacgtca aagcctgcgc cgcccaagcc gccgtatttc aaggcggcga tgagtgcggt
      181 ttcatcgacc aagccgccgc ggccaacgtt gatgatgacg caacctggtt tcaaggcttt
      241 caattctgct tcaccgagca aattgcgggt ttggtcgttg agtgggcaat gcaacgaaat
      301 cacatcggcc ctcgccaatg cctctgcaaa cggcacatag ccatcgcggc aagcgctggc
      361 gtgtttgtgt tcgccataaa tcaccttcat gccaaacgct tcggcgcgtt gcgccaaggc
      421 cgagcctatg ctgcctttgc cgaaaatcaa cagggttttg ccattcacat cgtggatggg
      481 cgcgccaaag tggcagaaat acggtgaatt ttgccacaag cctgcggcga catcgcgctg
      541 gtaggcaggc aattggcgca tcaaggccat catcaacatg aaagcgtgtt cggccacggt
      601 gtcgttacca taggctttga tgttggacac gattatgccg cgctcttgag cggcctgtac
      661 atccacatgc tcaaaacccg tggccgcgat ggcaatcatt ttcaggttgg ggctggcttc
      721 aattgttgcg cggtcgattt tgaccttgtt ggtgatgatg atttcggcat cgcgacagcg
      781 gttgatcgct tcattgtcag gcgtgtcatc gtgttcggtg aggtggtgtt caaaatcaaa
      841 tgcccatggc aaaggcatta aggtaccgcg gtctaaaacc acaatgtgac gactgctcat
      901 atttttcctt atcgataaaa ccaatcaaat gatttgtaaa gtttgttgca ctatttaaat
      961 aagaatagtt atcatttgta tttatatata cgttcacacc acgaggtgac cccatgatga
     1021 aaaccctgac ttttgccatc ttgcactttg gtgtggcatt cagtgtggct tatgccttga
     1081 cgggcagcat aggcatttcc agtaccgtag cattggttga acccattgtg aataccgtgg
     1141 tgttcttttt ccatgaaaag gcttggcagc gtattgaacg aaaacgccgc caaaaactag
     1201 gtttacaggc cgctttagca gaaaaaacag cccataagcc ttggtttggg tgtggccaca
     1261 gcctgtagtg caagcaggtg gtgagaagct tgtgctaaag tggggttttt gcaatggtag
     1321 aaggagtgaa catgggttta caggttgaag atttggtggt gggtgacggt gctgaggccc
     1381 agtcaggtaa ggaaatcact gtgcattaca cgggttggtt aacggatggt acacagtttg
     1441 attccagcat agaccgcaaa cagcctttga gtatcgtgct aggtgtgggc caagtcatta
     1501 agggttggga ccaaggcata gagggcatga aaatcggtgg caaacgcaaa ttgaccatcc
     1561 ctcctgagct cgcttatggc aaccacgccg tgggcggcgt gattccaaaa aacgccacgt
     1621 tggtgtttga ggtggagttg ttgggcgtgg cctaagtcat tgattgggtt ttgtttgacc
     1681 agaagcaagc cagcatttag ggcttgctta atttatgtca aagaggatga agatactgct
     1741 cagtattcat catgtttcta taatggttat gtcataattt ctttcaatgc aatgacttac
     1801 tgttgaaaag ggagttaaag acatggcggt ggtattgttc gatgacggtg tgcataaatg
     1861 tgtggcattc acagatttgg tggcaggtga aggggtacag gccaaccaat ttgccatttc
     1921 gcaccaaggt gagggcgtat tgctcgaccc tggtggcaat ttaacctata aacatttgtt
     1981 ggctgaaatt tccgattatt ttttgccttc caaaacccgc tatatctttg cctctcatcc
     2041 cgacccagac atcattgctt cggtgaatgg ctggctgttg attaccgatg cccaagtgtt
     2101 gattgcccaa gaatgggagc gttttcttcc ccatttttgt ttgaaagggg caacggttgg
     2161 gcgtttaaag accataccgc cgcaaggcat gtgtttggat ttgaatggcg gggagttgca
     2221 gattattcct gcccattttt tgcatcctgt tggcaatttt cacatttacg accccatcag
     2281 taagattttg ttttctggcg atgtgggcgc ttcgttggtg gatgccgaag aagccgcgca
     2341 aaccgtgagc gatttcaatc accatttaca taataacggc atgttgggct ttcatcagcg
     2401 ctacatgagt ggcaatatgg cgtgtcgtta ttgggtaaac atggtgcgtc aactggacat
     2461 agagtggata gttccgcaac acggcgcttc gttcaaaggc aaggaaatgg tgaaccaatt
     2521 cttggattgg tttgaaggtt tggcgtgtgg catcgattta ctcagccaag acgattttca
     2581 acttttgcct aagtaaatgt ctgacagtgg ggttaaatgg aaaagcgcta gagcaatatt
     2641 atagtgagtg aaaaataaga ataattactt aattgcgtat tttatctaat ttattaaata
     2701 ttatggccaa taatactgat ttttcttact ttaaatcaag caattcccat ttagcgctaa
     2761 tcaggttaaa acaaccgtgt tataacaagc tctcttaaaa atacagatac agttggaagt
     2821 cacatcatgg cagtggtttt gtatgacgac ggcgagcaca agtgtgtcgc cttttcagat
     2881 ttggtaacgg gtgaaggtgt acagtccaat cagtttgcgg ttgtgcacaa aggcgacggc
     2941 atgttgctcg atcctggagg taaccttact tataagcatt tgttggcaga gatttccgat
     3001 tacttcttgc catcgcacac gcgctatgtg ttcgcatcgc atgccgaccc agacatcatt
     3061 gcgtccatca atggctggtt gttgattacc gatgccaacg tgttgatagc aaaagaatgg
     3121 gaacgcttta ttccacattt ttgcttaaaa ggcgccacta ctggccgttt aatcagcatt
     3181 cccgcgccag gcatgaaagt agaattgcaa ggtgcagagt tgcatttgct gccagcgcat
     3241 ttcttgcatc cagtgggcaa tttccatgtg tatgacccca tcagcaaaat cttgttctca
     3301 ggtgacgtag gcgcttcttt ggtcgatgcg gaaaccgcag ccaaaccgat tgaagacttc
     3361 gaccaccatt tgcacaacaa tggcatgttg ggttttcaca agcgttacat gagtggcaac
     3421 aaagcttgtc gctattgggt gaacatggtg cgccaattgg atgtggaatg gatcgtgccg
     3481 caacacggtg cgtcgtttaa gggcaaggaa atggtcaacc gtttcttgga ttggttggaa
     3541 gacttggaat gcggcatcga tttgctcagc caagaggatt gacccctgct gtcaattccg
     3601 tacagttaaa cttgggagat t
//
LOCUS       DAOMVK010000209         3619 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_7494_length_3619_cov_4.350730, whole genome shotgun sequence.
ACCESSION   DAOMVK010000209 DAOMVK010000000
VERSION     DAOMVK010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7494_length_3619_cov_4.350730"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="PK856_07415"
     CDS             <1..918
                     /locus_tag="PK856_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HRL02233.1"
                     /translation="KNKVIMRWLAHRGGALDFLAHKQANPGQPYPVSVVLGCDPATIL
                     GAVTPVPDTLSEYQFAGLLRGSRTELVKCIGNDLQVPARAEIVLEGVIHPDETALEGP
                     YGDHTGYYNEQDHFPVLTIERITMRQNAIYHSTYTGKPIDEPAVLGVALNEVFVPLLQ
                     KQFPEIVDFYLPPEGCSYRMAVVSMKKQYAGHGKRVMMGCWSFLRQFMYTKFIIVVDE
                     DVDVRDWKEVIWAITTRMDPVRDTVLVENTPIDYLDFASPVSGLGGKMGMDATNKWPG
                     ETNREWGVVISKDAETSRRVDAMWSELGL"
     gene            1244..2434
                     /locus_tag="PK856_07420"
     CDS             1244..2434
                     /locus_tag="PK856_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRL02234.1"
                     /translation="MKKLLFAQVVTLALLTACGGGNTQTQTSSEAKPEAANPASATAA
                     ASNTQAGADLPQTDALNVYNWSNYMDESTWEDFKKEYSLKANYDTYENNEALEAKVLT
                     GKSGYDLVVPGIAFLPRQIKAGAYQKVHKDLIPNYKNIDPKLLKLLEQVDPGNEYAVP
                     YFSGLNTLAINKDKVKAALGTDQLPENEWDLVFKPEYTSKLKSCGISLWDSPSEIFPL
                     VVKYIGKNPADSNLDDLKAAAEVLKKVRPDIKRFSPSFIDELARGDICVAVGNGGDMN
                     MSKKRAEEVKNGVNIEVLTPKGMGFWVESWLIPADAKNIVNAHKYMDWTLRPDIAARN
                     AAEVTFAPASLPARDLMDKKLVETRSIFPNEADTAAGFLMPELSPDAKKLTVRLWQGL
                     RSGS"
     gene            2601..2900
                     /locus_tag="PK856_07425"
     CDS             2601..2900
                     /locus_tag="PK856_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02235.1"
                     /translation="MKKSLILLAALLGTVGLAQAEKINDYDNVRIKEQGTITRQTTTQ
                     TDNMPAQVTTSTQAVERKVSVDPAPNFTIDGQPAYEIKEEVTTTPVEVSTEIIPQ"
     gene            3161..>3619
                     /locus_tag="PK856_07430"
     CDS             3161..>3619
                     /locus_tag="PK856_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRL02236.1"
                     /translation="MSQYVFSMLRVSKVVPPQKQIIKDISLSFFPGAKIGLLGLNGSG
                     KSTVLRIMAGVDKEFEGEAIPMSGINIGYLPQEPELDPNKTVREEVESGLGAVGDAQK
                     RLDEVYAAYAEEDADFDALAEEQARLEAILSAGAGDNVEHQLEIAADALRL"
BASE COUNT         1024 a          902 c          847 g          846 t
ORIGIN      
        1 aaaaacaaag tcatcatgcg ctggctcgct caccgtggtg gcgctttgga ttttctggcc
       61 cacaagcaag ccaatcctgg gcagccctat cctgtgtctg tggtattggg ctgcgaccct
      121 gccaccattt tgggcgcggt cacacccgtg ccagacacct tgagtgaata ccaatttgca
      181 ggcttgttgc gcggctcacg caccgagttg gtgaagtgca taggcaatga tttgcaagtg
      241 cctgcgcggg cggaaatcgt gttagaaggc gtgatacacc cggatgaaac cgcgttagaa
      301 ggcccgtatg gcgaccacac cggctattac aacgaacaag accacttccc agtgctcacg
      361 atcgagcgca tcaccatgcg ccaaaacgcg atttaccaca gcacttacac cggcaagccg
      421 atagacgaac ctgcggtatt gggcgtggct ttgaacgaag tgttcgtgcc tttgttgcaa
      481 aaacagtttc ctgaaattgt cgatttttac ttgccgcccg aaggttgctc ttaccgcatg
      541 gcggtggtga gcatgaaaaa gcaatacgca ggccacggca agcgcgtgat gatgggctgc
      601 tggagcttct tgcgccaatt catgtacacc aaattcatca tcgtggtcga tgaagacgtg
      661 gatgtgcgcg actggaaaga ggtgatttgg gcgattacca cgcgcatgga tcctgtgcgc
      721 gacaccgtat tggtggaaaa cacgccgatt gactatctgg attttgccag cccagtgtca
      781 ggcttaggtg gcaaaatggg catggatgcc accaacaagt ggccaggcga aaccaaccgc
      841 gaatggggtg tggtcatcag caaagatgcc gaaaccagtc gccgtgtcga tgccatgtgg
      901 tctgaattgg ggttgtaaaa ccaaccctat ctccccataa cataccgaat acaagcggcc
      961 tgtaaactga gatttaaagt ttacaggccg ctttttatct gaatttcagc ctaccgaacc
     1021 cgcattaaaa ccatacccaa acctgctttc gtgtttctca ccacaaaata aaccccaaat
     1081 tcatttagca catgactgac tcggattaaa tacgctttaa tgattgcagc gcgaagttta
     1141 tcttttgatt cagcaatatg tgtatttccg ttataatcgg cgcggattgt gcctcaagca
     1201 ggcacaacgc ttttaatgaa ttcgacaggg aaagataacc tatatgaaaa aattgctctt
     1261 tgctcaagtt gtcacgttgg cattgctgac tgcatgcggt ggcggtaaca ctcaaactca
     1321 aacttccagt gaagccaaac ccgaagcagc caacccagcc tctgcaactg ctgcagccag
     1381 caatacccaa gccggtgctg atttaccgca aactgatgcc ttaaacgttt acaactggtc
     1441 gaactacatg gatgagtcga cttgggaaga ttttaaaaag gaatacagtt taaaagccaa
     1501 ttacgacacc tatgaaaaca acgaagccct cgaggccaaa gtgttgacag gcaagtctgg
     1561 ctacgatttg gtggttcctg gcattgcgtt cttgccacgc caaatcaaag cgggtgctta
     1621 tcaaaaagtc cacaaagatt tgattcctaa ctacaaaaac attgatccaa aattgttgaa
     1681 attattggaa caagtagacc caggtaacga atacgcggtg ccttatttct caggcttgaa
     1741 caccttggcc atcaataaag acaaagtcaa agccgctttg ggcacagacc aattgcctga
     1801 aaatgaatgg gacttggtat tcaaaccaga atacaccagc aaattgaaat cttgtggcat
     1861 cagcttgtgg gactcaccaa gtgaaatctt cccattggtg gtgaaataca taggtaaaaa
     1921 ccctgccgat tctaacttag acgatttaaa agccgctgcc gaagtcttga aaaaagtgcg
     1981 gccagacatt aaacgcttta gcccatcctt cattgatgaa ttggcgcgtg gcgacatctg
     2041 cgtggcagtg ggcaatggcg gcgacatgaa catgtctaaa aaacgcgccg aagaagtgaa
     2101 aaatggcgtg aacatcgagg tattaacgcc taaaggcatg ggtttctggg tggaatcttg
     2161 gttgattcct gccgatgcga aaaacatcgt caatgcccac aaatacatgg actggacttt
     2221 gagacctgac attgctgccc gcaacgccgc agaagtaacg tttgcacctg caagcttgcc
     2281 tgctcgcgac ttgatggaca aaaaactggt ggaaacccgc tctatcttcc ctaatgaagc
     2341 ggatacagca gcaggcttct tgatgccaga gctcagccct gacgcgaaaa aattgaccgt
     2401 gcgtttgtgg caaggtttac gtagcggttc ttaatctgct ggctctgtta tgaaatctca
     2461 caacccaaac atcacttgtt tgggttgttt ttttaacgca gcccaactgt aaagccttgt
     2521 acaagcgtgt aaagcctgca actacaattt agagcttcag tcataattac aaacgactta
     2581 attaatgcat aggaattgtg atgaaaaaaa gcttaatctt attggctgca ttgttgggca
     2641 ctgtgggttt ggcccaagcg gaaaaaatca acgattacga caatgtgcgc atcaaagagc
     2701 aaggcaccat tacccgccaa accaccaccc aaaccgacaa catgcccgca caagtgacca
     2761 cttctaccca agcggtggaa cgaaaagtca gtgtagaccc tgcaccaaat ttcaccattg
     2821 atggccaacc cgcctatgaa atcaaagaag aagtgaccac cacaccagtt gaagtcagca
     2881 ccgaaatcat ccctcaataa ggtgttgccc atttttacag gccgcttatg tgcggcctgt
     2941 cacttatttc acctcacgat ttgtgacctc tatctcggca ttattactga gacagaacaa
     3001 agctaaccct aacacatcac atccaattgc tgcttccaca cagattattc gcaaactcaa
     3061 agaaatagcc ccatctaaat ctcaagatta gtccctcgcc cactggcaag tatggcgcaa
     3121 ttgggtacaa tagccgtttt tattccaacg agacgacaac atgagccaat acgtattttc
     3181 catgctgcgc gtgagcaaag tggtgccgcc gcaaaaacaa atcatcaaag acatttccct
     3241 gtctttcttc cctggtgcca aaatcggctt attgggtttg aatggctcag gtaagtctac
     3301 cgtgttgcgc atcatggctg gcgtggacaa agagtttgaa ggcgaagcca tccccatgtc
     3361 tggcatcaac atcggctatt tgccgcaaga gccagaatta gaccctaaca aaaccgtgcg
     3421 cgaagaagtg gaaagcggct taggcgctgt tggcgatgca caaaagcgct tagacgaagt
     3481 ttacgcagcc tatgccgaag aagacgctga ctttgatgcc ttggccgaag aacaagcgcg
     3541 cttggaagcc attttgtccg caggtgctgg cgacaatgtg gaacaccaat tggaaatcgc
     3601 cgccgatgca ctgcgcttg
//
LOCUS       DAOMVK010000210         3608 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_7525_length_3608_cov_3.491416, whole genome shotgun sequence.
ACCESSION   DAOMVK010000210 DAOMVK010000000
VERSION     DAOMVK010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7525_length_3608_cov_3.491416"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1244
                     /locus_tag="PK856_07435"
     CDS             3..1244
                     /locus_tag="PK856_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HRL02237.1"
                     /translation="MLCAAGLVISSQSFAQTLSLRKDAPARYTVKSGDTLWSISRHYL
                     RSPWQWPQLWGVNRSHIRNPHLIYPGQTLVLRYVNGRPVLGFEDGGNIPTIKLTPGVR
                     TVSDGYGINTINMNLAQTFMRTPQIISTEVTKNAPYIIEGPDSRVIYSPGDRVYAYGL
                     AEPGIYYSYRVTKDIRDPDTNKFIGREVTITGKLEVLNYKPTAWEQREDDVNQRLNDD
                     EYYTKKHPLLKFPTTTAQPMVVREVSSEILKDDYLMKEPDFLTTGLHFMPHSPAAPID
                     AKVLSIIDGVSEAAQFQTIAINQGTVQGVDPGTVVSIYRKTKTHKIERKGKDSGPKYR
                     YISMPSEELALAMVYQANDNVSYALILDSLRNISIGDTVREPGRDLDDFPETTKHEPN
                     DPQEPHYYDNGRYNRGDYIKY"
     gene            1292..2395
                     /gene="dprA"
                     /locus_tag="PK856_07440"
     CDS             1292..2395
                     /gene="dprA"
                     /locus_tag="PK856_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRL02238.1"
                     /translation="MQTAERLASLELALTPKVGAMSFLKLLQAFSTAAAVWSASSTQL
                     QSIISSKAITAISTHQGQKALQAALTWESQHPDNHLLCLVDDDYPIFLSEIDSPPPIL
                     FARGDINLLQQPSVALIGSRHASASGLHISHDLAAELSRYGVCIVSGFAAGIDTAAHQ
                     GCVQAQGKTIAVLGTGIDRVYPAANRDLAHQISQSGLLLSEFPLGTAPIAHNFPRRNR
                     IIAGLAQAIIVVEATLESGSLITARLGLEMNKEVMAVPGSIHNPQSKGCHALIKQGAK
                     LVECVNDILEECPRLPQIRPVNELTKEKNTPTICINESSPLLKAMGFDPIHPDQLVDK
                     LGISATDVYAELLQLELQGKIAALSGGRYQRIT"
     gene            2411..2869
                     /locus_tag="PK856_07445"
     CDS             2411..2869
                     /locus_tag="PK856_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HRL02239.1"
                     /translation="MFDVIAFLIENFQDWETCPKQDDLGHLLAEAGFDGEEIHDALSC
                     LENLTSSLSMDNAILDDCPYVRVLSQYELDILPEDVIHLLSFLQEEGGISPTQRELII
                     HMLTQLPDEDITVDHTKLLALMVLWLHQSELPILIGDELLVALHGNTMMQ"
     gene            2968..>3608
                     /locus_tag="PK856_07450"
     CDS             2968..>3608
                     /locus_tag="PK856_07450"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HRL02240.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGADFEVLASYGHVRDLVPKSGAV
                     DPENDFAMKYQLVSRNSKHVDAIVQAAREAEHLYLATDPDREGEAISWHLQQILDGKR
                     GLKALPKERVVFHEVTKKAVLEAISNPRAISQELVDAQQTRRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIKAFTEQEYWSVHLDSHKGRSKFAAKLT"
BASE COUNT         1012 a          911 c          780 g          905 t
ORIGIN      
        1 ccttgctttg cgccgcaggc ttagtcattt catcgcaaag ttttgcgcaa acattatctt
       61 tacgtaaaga cgcgcccgct cgctacacag tgaaatcagg ggatacattg tggagcatct
      121 cgcgccatta tttgcgtagc ccatggcagt ggccacagct ttggggagtt aaccgttccc
      181 acatccgcaa tcctcatttg atttacccag gccaaactct cgttttgcgc tacgtcaacg
      241 gacgtccagt gctgggtttt gaagatggtg gcaacatccc aaccatcaaa ttaactcctg
      301 gtgttcgtac agtttccgat ggctatggca tcaacaccat caatatgaat ttggcgcaaa
      361 cctttatgcg caccccccaa atcatctcaa cagaagttac taaaaatgcc ccttacatca
      421 ttgaaggccc agacagtcgc gtaatttatt cacctggtga tcgtgtatac gcctatggct
      481 tggctgaacc aggtatttat tacagctacc gcgtgaccaa agacatacgt gatccagata
      541 ctaataaatt tattggccgt gaagtgacca ttacgggcaa attagaagta ttgaattaca
      601 aaccaacggc ttgggaacaa cgtgaagatg acgtgaatca acgtttgaat gacgatgagt
      661 attacaccaa gaaacaccct ttactgaaat tcccaaccac caccgcccag cctatggtag
      721 ttcgtgaagt gtcttccgaa atattaaaag atgactactt aatgaaagaa cctgactttt
      781 taaccacggg tttacatttc atgccacact caccagcagc cccaattgat gccaaggtat
      841 tgtctatcat tgatggcgtc agtgaagcag cccaattcca aaccatagct attaaccaag
      901 gtacggttca aggcgtcgac ccaggcacag tggtgagcat ttaccgtaaa accaaaaccc
      961 acaaaattga gcgcaaaggc aaggacagcg gccctaaata ccgttacatt tctatgccat
     1021 cagaagaatt ggctttggcc atggtatacc aagccaatga caacgtatct tatgccttga
     1081 ttttagacag tcttcgcaac atcagcattg gtgatactgt gcgcgaacca ggccgcgatt
     1141 tggatgattt cccagaaacc accaaacacg aacctaacga tccacaagag cctcattatt
     1201 acgacaatgg ccgctacaac cgcggtgatt acatcaagta ctgactttca gagcgcacct
     1261 tcaaggtgcg cttttttgtg gagactatcc catgcaaacc gcggaacgtt tggctagtct
     1321 ggaattggca ttaaccccaa aagtgggggc gatgtctttt ttgaaactac tgcaagcttt
     1381 ctccacggcc gctgcggtgt ggtcagcttc ctccactcaa ttgcaatcaa taatttccag
     1441 taaagccatt actgccatct ctacccatca agggcagaaa gcattacagg ctgctttaac
     1501 atgggaatca caacaccctg acaaccattt gctttgctta gtagacgatg attaccccat
     1561 ttttttaagt gaaatcgatt ccccaccacc catattgttc gcacgaggcg acatcaactt
     1621 acttcaacaa cccagtgtcg ctttaattgg cagccgacac gccagtgcca gtggcttaca
     1681 catttcgcat gacttagcag ctgaattaag ccgctatggt gtctgcattg tttctggatt
     1741 cgccgcaggc atagacacag ccgctcacca aggttgtgtt caagcccaag gcaaaaccat
     1801 tgcagtactt ggaacaggca tagaccgtgt ctaccctgct gctaaccgag acttggctca
     1861 ccaaatcagt caatcaggtt tactgctatc cgaatttcca ttgggcaccg cccctattgc
     1921 tcacaacttc ccacgtcgca accgcatcat tgcgggtttg gctcaggcca ttattgtggt
     1981 cgaagcgact ttagaatccg gctcattaat caccgctcgt ttgggtttgg aaatgaacaa
     2041 agaagtgatg gcagtgcctg gttctatcca caatcctcaa agcaaaggct gccatgcctt
     2101 aatcaaacaa ggcgcaaaac tggtggaatg cgtaaatgat attttagaag aatgcccacg
     2161 cttgcctcaa atacggcctg taaatgaact tactaaggaa aagaacacac caacaatatg
     2221 tattaatgaa tcatcaccac tcttgaaagc catggggttt gaccccatac atcctgacca
     2281 actggtcgat aaacttggca tcagcgccac agatgtgtat gctgaactcc tgcagttaga
     2341 gttacaaggc aagattgctg ctctttcggg cggccgttac caacgaatta cttgataaag
     2401 tggatggatt atgttcgatg tcattgcctt cttaattgaa aacttccaag attgggaaac
     2461 ctgtcccaaa caagatgatt tgggtcactt gttggctgag gctggttttg acggcgaaga
     2521 gatacacgat gccttaagtt gcttggaaaa tctaaccagc agcttgagta tggacaatgc
     2581 cattttagac gattgtccgt atgtgcgtgt cttaagccaa tatgagctgg acattttacc
     2641 cgaagacgtg attcatttac tgagcttctt acaagaagaa ggcggcatca gcccaacaca
     2701 acgtgaactc atcattcaca tgctgacgca acttcctgat gaagacatca ctgtagacca
     2761 caccaaatta ctggcattaa tggtgttatg gctacaccaa agcgagttgc ctattttgat
     2821 aggcgatgaa ttattggttg ccttgcatgg caataccatg atgcaataat ggttaggtta
     2881 gcaaatacct accccaaaac gcagccattt cgctgcgttt tgctcacttt gcctcacaca
     2941 aaataaccaa gtgaataaga caaaaacatg gcgaaaaacc tactgattgt ggagtctccc
     3001 tccaaagcca aaaccttgaa aaaatacttg ggtgccgact ttgaagtctt ggcatcgtat
     3061 ggtcatgtgc gcgatttggt tcccaaaagt ggtgcggttg accctgaaaa cgattttgcg
     3121 atgaaatacc aattggtcag tcgcaacagc aaacacgtcg atgccatcgt acaggccgct
     3181 cgtgaagccg aacacctcta cctcgcaacc gacccggata gggaaggtga agcgatttct
     3241 tggcatttac agcaaatcct cgatggcaaa cgcggcctga aagccctgcc caaagagcgc
     3301 gtggtgttcc atgaggttac caaaaaagcc gtcttggaag cgatttccaa tccgcgtgcc
     3361 atttcgcaag aattggtcga tgcccagcaa acccgccgcg ctttagacta tttggtgggt
     3421 ttcaatctat cgcccttgtt gtggaaaaaa atccgccgtg gcttgtcggc aggtcgtgtg
     3481 caaagccctg ctttgcgcct gatttgcgaa cgtgaaaacg aaatcaaagc ctttaccgag
     3541 caagagtatt ggtctgtgca tttggacagc cacaaaggcc gcagcaaatt tgccgccaag
     3601 ctcaccca
//
LOCUS       DAOMVK010000211         3603 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_7539_length_3603_cov_2.395434, whole genome shotgun sequence.
ACCESSION   DAOMVK010000211 DAOMVK010000000
VERSION     DAOMVK010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7539_length_3603_cov_2.395434"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PK856_07455"
     CDS             <1..333
                     /locus_tag="PK856_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRL02241.1"
                     /translation="GSVITQFNSSNKGIDIAGNAGQPVVAAADGKVVYSGSDLRGYGN
                     LIIVQHNTTFLTAYGHNQSLLVKENDNVKRGQTIAYMGNSHTDRYKLHFELRQNGKPV
                     NPLDFIPN"
     gene            417..>659
                     /gene="rdgC"
                     /locus_tag="PK856_07460"
     CDS             417..>659
                     /gene="rdgC"
                     /locus_tag="PK856_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HRL02242.1"
                     /translation="MWFKQVSFFVLPQDAELNLEDIQTKLEEASFKPCQGLDWKSEGF
                     ARAVPFREDLVFNAQDSWRISLRQEDKVLPASVIREI"
     assembly_gap    660..669
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <670..1315
                     /locus_tag="PK856_07465"
     CDS             <670..1315
                     /locus_tag="PK856_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HRL02243.1"
                     /translation="AEIQANDGRNVGRKEKMDLKEQITDDLLPRAFTRSRYTEGLIDV
                     PGQLLLVNQSNSNKAENFVSQLRQALGSLPASLPRTNESPSSLMTAWLEQGEAAGNFE
                     LDSDCELKGVGDAAPVIKISKQDLAADEVKQHLEHGKVCTQLGLIWNEQVRFVLSEDL
                     SLKRIQYLDMLQEEASNQGDDMESLMTATQIIMTQNLSLLIHELIEHLGGLQAA"
     gene            1326..1892
                     /gene="dcd"
                     /locus_tag="PK856_07470"
     CDS             1326..1892
                     /gene="dcd"
                     /locus_tag="PK856_07470"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HRL02244.1"
                     /translation="MSIKSDKWIRRMSEEHGMISPFEPQQIKQLNGEKIISYGTSSYG
                     YDIRCAREFKIFTNINSTIVDPKNFDTRNFVEIEDDYCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYANEGVAQVLFF
                     ESDEDCETSYKDRNGKYMGQVGVTLPKA"
     gene            2075..2557
                     /locus_tag="PK856_07475"
     CDS             2075..2557
                     /locus_tag="PK856_07475"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HRL02245.1"
                     /translation="MSIYSHSIQSLTGESLALSQFEGQALLLFNSASQCGFTPQLSHF
                     EYLNEQYACQGLRIIGFPCNQFKQQEPGSPADIHTFCQKNFGVTFLMSEKIDVNGKHT
                     HPLWQELKASKRGILGTQSIKWNFTKFLIAPDGEIILRAAPYTNPLKLIPHIEKLISK
                     "
     gene            2679..3518
                     /gene="serB"
                     /locus_tag="PK856_07480"
     CDS             2679..3518
                     /gene="serB"
                     /locus_tag="PK856_07480"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HRL02246.1"
                     /translation="MAVLVLQNPHLAQLNWQTWSNVFPIHDTQNHSVWRIQVADDFTL
                     TVEQNQWLTQHQVDAAVLPDIPFSDLGLIISDMDSTLITIECIDEIAAGNGLKDQVSA
                     ITERSMAGELDFASSLRERVALLKGLPESELAHVYDHVLQLTSGAETLIAACKQHGVK
                     FMLVSGGFTYFTERLKTQLGLDYAYANELEIVDGKLTGKLTGRMIDAQAKADLLQQHA
                     NELNIPLSHTLAMGDGANDIPMIQAAGFGVAFHAKPKTRSMADICIDFGGLDAIYHCF
                     KPA"
BASE COUNT         1054 a          841 c          747 g          951 t
ORIGIN      
        1 ggcagcgtga ttacccaatt caacagctcc aataaaggca ttgatattgc tggtaacgca
       61 ggccaacctg ttgtggcagc ggccgacggc aaagtggttt acagtggcag cgatttgcgt
      121 ggctatggca atttgattat tgtgcaacac aacaccacct tcctcaccgc ttatggccac
      181 aaccaaagcc tgttggtgaa agaaaatgac aatgtaaaac gcggtcaaac cattgcctac
      241 atgggcaatt cccacaccga ccgctacaaa ttgcatttcg aattgcgcca aaatggcaaa
      301 ccagtgaatc cattggattt catccctaat taacaagtaa gcggcctgta aaagcttaca
      361 ggccgctttt ttgtcatcaa tatcgcctgc gtgcgatatt tttatttgag gctattatgt
      421 ggtttaaaca agtcagcttt ttcgtattac cccaagacgc cgaattgaat ttagaagaca
      481 tccaaaccaa actcgaagaa gccagcttca aaccttgcca agggctggat tggaaaagtg
      541 aaggctttgc ccgcgctgtg ccatttcgtg aagacttggt ctttaatgcc caagacagct
      601 ggcgcatcag cttgcgccaa gaagacaagg tattgcctgc cagcgtgatc cgcgaaattn
      661 nnnnnnnnnc gccgaaatcc aagccaacga cggtcgcaat gtcggtcgca aagaaaaaat
      721 ggatttaaaa gagcaaatca ccgatgattt actgccccgc gccttcaccc gctcacgcta
      781 taccgaaggc cttattgatg tacctgggca attgctattg gtcaaccaaa gcaacagcaa
      841 caaagctgaa aattttgtca gtcaattgcg ccaagccttg ggcagtttgc ctgcatcatt
      901 accgcgcacc aatgaatcac caagctcatt gatgactgct tggttagagc aaggtgaagc
      961 cgcgggcaat tttgaattgg acagcgactg cgaactcaaa ggcgtgggcg atgccgcccc
     1021 tgtcatcaaa atcagcaagc aagatttggc ggctgacgaa gttaaacaac acctagaaca
     1081 cggtaaagtg tgcacacaac tgggcctgat ctggaatgaa caagtccgct ttgtgttaag
     1141 cgaagactta agcttgaagc gcatccagta cttagacatg ttgcaagaag aagcctcaaa
     1201 tcagggcgat gacatggaga gcctgatgac agccactcaa atcatcatga cccaaaatct
     1261 aagcctgtta attcatgaat taattgaaca cttgggcggc ttacaagcgg cctgaaagag
     1321 acactatgag cataaaatcc gacaaatgga tacgccgcat gagcgaagag cacggcatga
     1381 tttccccatt tgaacctcaa caaatcaagc aattaaatgg cgaaaaaatc atttcctatg
     1441 gtacgtctag ctatggttat gacatacgtt gcgcacgtga atttaagatt ttcaccaaca
     1501 tcaattccac cattgtcgat cctaaaaact tcgacacccg caattttgtg gaaattgaag
     1561 acgattattg catcatcccg cccaactcat ttgcgttggc tcgcacagtg gaatacttcc
     1621 gcattccacg caatgtcttg acagtttgtt tgggtaaatc gacttatgct cgctgcggca
     1681 ttattgtgaa cgtgacccct tttgagcccg aatgggaagg ttatgtgacc ttagaattct
     1741 caaacacaac tccactaccc gcaaaaatat acgccaacga aggcgtggcg caggttttgt
     1801 tttttgagag tgatgaagac tgtgaaacct cttacaaaga cagaaatggc aagtacatgg
     1861 gccaagttgg tgtcacctta cctaaggctt aagctctaat tatatttact tgcctttgca
     1921 atttaatgct gcttttttgc agaaaaatag gtagaatggt ttctgcaaat gtttcctcct
     1981 gaatattttg cattattccc ttattttgaa ttgatattca aacgctgtat tggctattcc
     2041 ccttgccgta cagcgtattt ttttggaggc aaacatgagt atttattctc actccatcca
     2101 aagtctgact ggtgaatctt tggctttaag ccaatttgaa ggtcaagcct tgttgttgtt
     2161 taattctgcc agtcaatgtg gatttacccc acaattatcc cattttgaat acttgaatga
     2221 gcaatatgca tgccaaggtt taaggattat tggttttcca tgcaatcagt tcaaacagca
     2281 agaacctggc tcacctgctg acatccacac gttttgccaa aaaaatttcg gtgttacctt
     2341 tttaatgtct gaaaaaattg atgttaatgg caaacacacc catcctttgt ggcaagaatt
     2401 gaaagccagc aaacgcggca ttttgggcac tcaaagcatc aaatggaact tcaccaaatt
     2461 tttaattgct cccgatggcg aaatcatttt aagagcagct ccttacacca acccgctcaa
     2521 acttattcct cacattgaaa aacttatatc gaaataagcc agcccaattc acactccttt
     2581 cacaacccaa ctcaatttat caccattaaa atgacgctac gtgaggctta taccatgaat
     2641 ttatggcaaa ataagccttt tatttggcgg tgatgatcat ggctgttttg gtattacaaa
     2701 accctcattt ggcacaactg aattggcaga catggtcaaa cgtgtttccc atccacgata
     2761 cccaaaacca cagtgtttgg cgcattcaag tagccgatga ctttacttta accgttgaac
     2821 aaaaccaatg gttaacacaa caccaagttg atgcagcagt cttacctgat attcctttct
     2881 ctgatttggg cctcatcatc agcgacatgg actccacact gattaccata gagtgcatcg
     2941 atgaaattgc cgcaggcaac ggactcaaag accaagtgtc agcaatcact gaacgctcta
     3001 tggcaggaga attggatttt gccagctctt tgcgggaacg tgtggccttg ttgaaaggtt
     3061 tgcctgaatc cgaattggcc catgtatacg accatgtttt gcaattaacc tcaggagctg
     3121 aaaccttgat agcggcctgt aaacaacatg gagtgaaatt catgctggtt tcaggtggat
     3181 tcacttactt tactgaacgt ttaaagactc aactggggct cgattatgcc tatgccaacg
     3241 aattggaaat cgtagatggc aaactcacag gcaaacttac aggccgcatg atagacgctc
     3301 aagccaaggc cgatttactg caacaacatg ccaatgaatt gaacattcca ttgagccaca
     3361 ccttggccat gggcgatggt gccaatgaca tcccaatgat acaagccgca ggctttggtg
     3421 tggccttcca tgccaagccc aaaacccgca gcatggcgga catttgcatt gattttggtg
     3481 gactggatgc catttaccat tgctttaagc ctgcataatt tctatcttaa acttcttagg
     3541 cacgacttaa tagttgactg aattagtcgg aaataagata atgttcacat attcaagcag
     3601 gaa
//
LOCUS       DAOMVK010000212         3597 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_7557_length_3597_cov_3.413326, whole genome shotgun sequence.
ACCESSION   DAOMVK010000212 DAOMVK010000000
VERSION     DAOMVK010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7557_length_3597_cov_3.413326"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..128)
                     /locus_tag="PK856_07485"
     CDS             complement(<1..128)
                     /locus_tag="PK856_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabZ"
                     /protein_id="HRL02247.1"
                     /translation="MTEYALPIEAKTLQTLLPHRYPFLLLDRVTAFEANQTLTAIK"
     gene            complement(141..1181)
                     /gene="lpxD"
                     /locus_tag="PK856_07490"
     CDS             complement(141..1181)
                     /gene="lpxD"
                     /locus_tag="PK856_07490"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRL02248.1"
                     /translation="MKLSEIVAKIGGQLQGIDKDVLRLAPLESADASAIAFLSNPKYH
                     QQLLDTQAAAVIIHTKVEDTPAHVSVIRTDDPYLYFAQLAWLFHPRAQASGKRHPTAM
                     VEDSAVVPDSCEIGANVYIGEHTVLGENCRVLAGAVIEYDCVLGGDCIIHPNVTLYHG
                     CTLGQRVEIHSGTVIGGDGFGYAPVKTDGSWFKIPQTGGVTLADDVEIGSNSTVDRGA
                     MEDTRIGRGSKIDNFVQIAHNCQIGEHTVIASCTGISGSCKIGNHVVIAGGVGMAGHL
                     TIADKTFIGGGTNITKSITEAGHYASVYPMQTFKEWSKNAVYVRRLADMNGRIKQLEQ
                     HIASLTGEPSNN"
     gene            complement(1186..1686)
                     /locus_tag="PK856_07495"
     CDS             complement(1186..1686)
                     /locus_tag="PK856_07495"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRL02249.1"
                     /translation="MRSWLMSAGVAVALMGTAASAWAEAVQKIGFVDTDRVYRESKDA
                     QAINTTLEKEFGTQFKHLRQLEKQGVELQKLLLSNKLAASEQKRQQERMAKINKEYLT
                     LKSSTNEEYSLRRNEEFASMQQKANQALIELAKAENYDVIVKDVVYVRSDFDITDKLI
                     KIMNRN"
     gene            complement(1701..>3597)
                     /gene="bamA"
                     /locus_tag="PK856_07500"
     CDS             complement(1701..>3597)
                     /gene="bamA"
                     /locus_tag="PK856_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HRL02250.1"
                     /translation="TPKVTRLDRNRVEIELTIDEGETTTIKNIEFDGNEKFSDRTLRN
                     QISHTESGLFTWFRKSNRFSQDKMPADLESLSEFYQNRGYFDFKVTNAEVLPNPDDKT
                     ELNMKISVNEGQRYRFGQVAVAGDLLDVPAEEINDLNKIKAGKWYERSQVMDLVRSVQ
                     STMGNYGYAYTSVEVQPRPNAADGIVDFTVMVNPGRKIYVNQINIAGNNKTRDEVVRR
                     ELRQMENATYDQAKITRSKERVQQLGYFDNVKVDAVPVPNSPDQVNLDMAMTERSTGS
                     IDAALGYVQDDGIVLSGGIAQDNLFGTGKSASLRLSNSESSKAAALSFTDPYFTPDGV
                     SLGYDVFYREYDAREAEVSNYKTSTMGAGIRMGVPVTEYDRVNFGFTPEWMKLKLYDG
                     APKRYTDFVEQYDGKGSTSADFLTLKGNVGWGRNKTDSALWPTRGYIMKANAEIGLPG
                     GDIQYYKLTHGQTWFFPLSKNLTLMLNGEVGYADGYGKTKELPFFHNFYGGGLGSVRG
                     YESSSLGPKEPNGYDDDYDYMGGSMKAVANAELLFPLPGIKDQRSVRLSLFADAGSVW
                     DNDQHGKNDGTSYKSTFTEELRYSAGAAFTWLSPMGPLKFSYAYPLNKKDGDEEQRFQ
                     FQLGTTF"
BASE COUNT          946 a          899 c          739 g         1013 t
ORIGIN      
        1 tttttgatgg cggttaaggt ttgattggct tcaaaagcag tgacgcgatc aagcagcaaa
       61 aatgggtaac gatgcggtaa caaagtttgc aaggttttgg cttcaatcgg taatgcatat
      121 tcagtcatgc ttgagtatcc tcagttgtta cttggctcgc ctgtgagcga tgcgatgtgt
      181 tgttcaagtt gtttgatgcg gccattcatg tcggcaaggc ggcgcacata caccgcattc
      241 ttgctccact ctttgaaggt ttgcatcgga taaacagagg cataatgccc tgcttcagta
      301 atggatttgg tgatgttggt tccaccgcca ataaaggttt tgtcggcgat ggtcaaatgc
      361 ccagccatgc ctacaccacc agcaatcacc acgtgattgc ctatcttgca actgcctgaa
      421 atgcctgtgc aagacgcaat cacagtgtgc tcaccgattt ggcaattgtg ggcaatttgc
      481 acaaaattgt cgattttact gccacgaccg atgcgtgtgt cttccatcgc accgcggtct
      541 acggtggaat tagagccaat ttcaacatca tcggccaaag tcacaccacc tgtttgtgga
      601 attttaaacc atgagccatc ggttttcaca ggtgcatagc caaagccatc gccaccaatg
      661 acagtgcccg aatgaatttc cacccgttgg cccaaagtac aaccatggta aagcgtcacg
      721 ttggggtgga tgatgcaatc gccgccaagc acgcaatcat actcaatcac ggcgcctgcc
      781 aacacgcgac agttctcacc caatactgtg tgctcaccaa tgtaaacatt cgcaccaatt
      841 tcacaactgt caggaaccac tgcagagtct tctaccatgg ctgttggatg tcgcttgccc
      901 gaagcttgcg ctcgtgggtg aaacaaccaa gccaattgag caaaatacaa atacggatca
      961 tcagtacgta tcacactcac atgtgcaggc gtgtcctcaa ccttggtgtg gatgatcacc
     1021 gctgcggctt gggtatccag caattgttgg tggtattttg ggttacttaa aaatgcgata
     1081 gccgaagcat cagcactttc caatggcgcc aacctcagca catctttgtc aatcccttgc
     1141 agctgaccac ctatttttgc gactatttca gataacttca tatttttaat tgcgattcat
     1201 gattttaatc agtttgtcgg tgatgtcaaa atcagaacgg acgtacacca catctttcac
     1261 tatcacgtca tagttctcag ccttggccaa ctcaatcaaa gcctgattgg ctttttgctg
     1321 catagaagca aactcttcat tgcgacgcaa agagtattct tcattggttg atgatttcaa
     1381 tgtcaaatat tctttgttga ttttggccat gcgttcttgt tgacgttttt gctcactggc
     1441 agccaactta ttggaaagca ataatttttg taattccaca ccttgctttt ccaactggcg
     1501 caaatgctta aattgcgtac caaactcttt ttccaaagtt gtgttgatgg cttgagcatc
     1561 tttggattcc cgatacaccc tatcagtgtc aacaaaaccg attttttgta ctgcttctgc
     1621 ccaagctgag gcagcagtgc ccattaaggc cactgccaca cctgctgaca tcaaccatga
     1681 acgcatattc acataccttt ctagaaggtt gtacccaatt ggaattggaa acgttgctct
     1741 tcatcgccat cttttttgtt caaaggatag gcgtaactga atttcaacgg acccattggg
     1801 gataaccacg taaatgccgc acctgctgag taacgcaatt cttcagtaaa tgtgctcttg
     1861 tagctcgttc catcattttt accgtgttga tcgttatccc aaacagaacc agcatcggca
     1921 aacaaactca aacgcactga gcgttggtct ttaatgcctg gtaatgggaa caacaattcc
     1981 gcattggcca cggctttcat gctaccaccc atgtagtcgt aatcatcgtc atagccattt
     2041 ggttctttcg gccccaagct gctgctttca taaccgcgca ccgaacccag accaccacca
     2101 tagaagttat ggaagaacgg caattctttg gttttaccat agccatctgc ataacccact
     2161 tcgccattaa gcattaaagt taagttcttg cttaaaggga agaaccaagt ttgaccatgt
     2221 gtcaatttgt aatactgaat gtcaccacca ggtaagccga tttctgcatt ggctttcatg
     2281 atgtaacctc gggtcggcca caaagcactg tcagttttgt tacgccccca acccacattg
     2341 ccttttaagg tcaaaaagtc tgctgaagta gagcctttac catcatattg ctccacaaag
     2401 tcggtatagc gtttcggcgc accatcatac aacttcaatt tcatccattc aggagtgaaa
     2461 ccaaaattca cacggtcata ctcggtcaca ggtacgccca tgcggatacc tgcacccatg
     2521 gtagaggtct tgtaattact gacttcggct tcacgtgcat cgtattcgcg gtagaacacg
     2581 tcataaccca agctgacacc atcaggagta aagtacgggt cagtaaacga caatgctgcg
     2641 gccttagaag attcgctgtt agacaaacgc aaactggcag acttgcccgt accaaacaaa
     2701 ttatcttggg caataccacc ggataacacg ataccatcgt cttgtacgta acccaacgcc
     2761 gcatctatgg agcctgtaga gcgttcagtc attgccatgt ccaaattcac ttggtctggt
     2821 gagttaggta cgggcacagc atccactttg acattatcga aataacccaa ttgctgcaca
     2881 cgctctttag atcgggtgat tttggcttga tcgtaagtgg cattttccat ttgacgcaac
     2941 tcacggcgta ccacttcatc acgggttttg ttattacccg caatattgat ttgattcaca
     3001 taaattttac ggcctggatt caccatcaca gtaaaatcca caataccatc agcagcattt
     3061 ggacgcggct gcacttcaac cgaggtatac gcataaccat aattgcccat ggtggattgc
     3121 acgcttcgca ccaaatccat gacttgactg cgttcatacc atttacccgc tttgattttg
     3181 ttcaaatcat tgatttcttc ggctggcaca tccaataaat caccagcaac agccacctga
     3241 ccaaaacggt agcgctgacc ttcattcacc gaaattttca tattcaattc ggttttgtca
     3301 tccggattgg gtaatacctc ggcattggtc actttaaaat caaaataacc gcggttttgg
     3361 taaaactcac ttaagctttc caaatctgca ggcattttgt cttgggaaaa acggttagac
     3421 ttgcggaacc aagtaaacag gccgctttct gtatgactga tttgattgcg caaagtgcga
     3481 tcagagaatt tttcgttacc atcaaattca atgtttttga tggtggtggt ttcaccctca
     3541 tcaatggtca attcaatttc aacacggttg cggtctaaac gagtcacttt tggcgtg
//
LOCUS       DAOMVK010000213         3576 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_7608_length_3576_cov_2.984947, whole genome shotgun sequence.
ACCESSION   DAOMVK010000213 DAOMVK010000000
VERSION     DAOMVK010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7608_length_3576_cov_2.984947"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..849)
                     /locus_tag="PK856_07505"
     CDS             complement(130..849)
                     /locus_tag="PK856_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRL02251.1"
                     /translation="MTKILLVEDDAAIAEPLSYALKRETWEVTWCPTAQQALDFLNEN
                     QVDFMILDVGLPDMDGFELCRQIRKTNNTPLLFLTARTDEIERIIGLEIGADDYCAKP
                     FSPRELISRIKAILRRVQPPAPAPVVINNTDTLSVPAHPDDVLLPPWHYQAARQQVSY
                     DGQMLALTRNELNLLVAFLRRPNWVLSREQLMQAAWPHPDHSLLRTVDTHIKTLRQKL
                     KSAEAQESIVTHRGLGYELRH"
     gene            1072..1590
                     /locus_tag="PK856_07510"
     CDS             1072..1590
                     /locus_tag="PK856_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003778705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRL02252.1"
                     /translation="MKLTPMSEKFILHWGEMGTQWGVNRTVAQIHALLFIAARPMAAD
                     EICDTLGVARSNVSNSIKELQSWRLVTVVHQLGDRRDHFETNGEVWELFRVIAAERQR
                     REIEPTERFLTEMMASPEFAQQDKEVQRRITQTHELISTLTVWTGQMLKLSTATLVRL
                     LKLGSSIQKFLK"
     gene            1607..1747
                     /locus_tag="PK856_07515"
     CDS             1607..1747
                     /locus_tag="PK856_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02253.1"
                     /translation="MSQVDDIIKNHLQAASAEQRHVYVACSNYDDNLPHLQYLAAQAD
                     LD"
     gene            1760..2089
                     /locus_tag="PK856_07520"
     CDS             1760..2089
                     /locus_tag="PK856_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02254.1"
                     /translation="MMFWCLGGRYLAQFAPEHAKYQSEDAQLCRELITRLQQGFYPDA
                     NIYFDPEQDSPVRSSEYSDCPDVWQIPAMMYAVVMGSEYVDLDDDAYDDGLPLTVATA
                     VWNADYD"
     gene            2240..3103
                     /locus_tag="PK856_07525"
     CDS             2240..3103
                     /locus_tag="PK856_07525"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HRL02255.1"
                     /translation="MNILLLGSTGLIGSAVHAKLSATTHTVIAPTSAELDFQRPDTWA
                     AWLQQADVLVNCVGVMSADSGLMQSVQCTAPQALYQQAQRAGMGYVLQVSALGASVDA
                     DTVFLRSKGQLDAWLLASCLNVGIVRPSLVFAKTGRSSRLFVNLAKLPHLWLPEAGHM
                     VIQPLTIQPVALNDVATAVVAMLQSRYSGVVNAVGGKALSLAAYLTLLRQYAWQRGPA
                     HIHALPVTWAKRAAEVLQHVSSGLITPASIEMLLESEEVEVEDFARILGRQPQTPAQF
                     LQAAYAQGAHK"
     gene            3100..3465
                     /locus_tag="PK856_07530"
     CDS             3100..3465
                     /locus_tag="PK856_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX-like family protein"
                     /protein_id="HRL02256.1"
                     /translation="MNMRKVMVAALVVMWLYSGLQPILTVPTESLQMLARVGVSAAWQ
                     WPLLLAASVCDVAVALWIVWRPSRTLWWLQCGLVAFYSVVIALWLPENWLHPFGPLIK
                     NLPIMAMMAHLATTEARKV"
     gene            3467..>3576
                     /locus_tag="PK856_07535"
     CDS             3467..>3576
                     /locus_tag="PK856_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003778699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HRL02257.1"
                     /translation="MEYYLWVKALHILSSVLMVGTGFGTAFYLFWANRSGS"
BASE COUNT          862 a          810 c         1008 g          896 t
ORIGIN      
        1 cggccaaaat gtcttccatc accttgcggg tattcggttt cacctgttct tgaaactgat
       61 gcaccatcat gatgccgcca caaatcaaaa tcaccgtgaa gagtagccaa atgcggataa
      121 aaatgggcat taatgtctca attcatagcc cagtccacgg tgggtcacaa tggattcttg
      181 tgcttcggca gactttaatt tttgacgcag ggttttgatg tgggtatcga cagtgcgcaa
      241 caagctgtgg tcaggatgtg gccatgcggc ctgcatcaat tgctcacggc tgagtaccca
      301 gttgggacgg cgcaggaaag ccaccaacaa attcaattca ttgcgggtca gtgcaagcat
      361 ctggccatcg tagctgactt gctgtcttgc ggcttggtaa tgccaaggtg ggagcagtac
      421 atcatcggga tgggcaggca cactgagtgt gtctgtgtta tttatgacca caggcgcggg
      481 agcagggggc tgtacgcggc gcaaaatggc tttgatgcga gaaatcaatt cgcgcgggct
      541 aaaaggcttg gcgcaataat cgtcggcacc gatttccagg ccgataatgc gttctatttc
      601 atcggtgcgt gcggtcagaa acaacaacgg cgtgttattg gttttgcgga tttggcggca
      661 caattcaaag ccatccatgt ctggcaaacc cacatccaaa atcatgaaat ctacttggtt
      721 ctcgttcaaa aaatccagtg cttgttgcgc agttgggcac caagtcactt cccaagtttc
      781 gcgttttaaa gcgtagctta gtggttctgc aatcgcagca tcgtcttcaa ccagtagaat
      841 ttttgtcatg gtagcttgca atcatggggt gatttaacaa tgaggcctat tctaatggag
      901 tcttgcacta ttttcagctg tatttattgg gattcatcca tgcgcccagc atatgggtat
      961 ctgcgcagca tttgttgcaa cttagagaaa gtttcaaaac attgcttaat ttctgtattg
     1021 acagaaatta tgagaggttt taccatgtgt tcaaagtaag gagaatccaa catgaaactg
     1081 acaccaatgt ccgaaaaatt cattttgcac tggggtgaaa tgggcacgca atggggtgtt
     1141 aaccgcacag tggcgcaaat tcacgcctta ttgttcattg ccgcacgccc gatggcggcg
     1201 gatgaaattt gcgatacctt gggcgtggca cgttccaatg tcagcaacag catcaaagag
     1261 ctgcaaagct ggcgtttggt gacggtggta caccagttag gcgatcgccg tgaccatttt
     1321 gaaaccaatg gcgaggtgtg ggaattgttc cgtgtgattg ccgcagagcg ccagcgccgt
     1381 gagattgagc ctactgagcg ttttctgact gaaatgatgg cgagccctga gtttgcccag
     1441 caagacaaag aagtgcagcg tcgcattacc caaacccatg agttgatttc caccttaacc
     1501 gtgtggacag ggcaaatgct caagctctcc accgccactt tggtgcgctt attgaaactg
     1561 ggcagcagca ttcaaaaatt cttgaagtaa acgcaaggag agaggcatga gccaagtcga
     1621 tgacatcatc aaaaaccatt tacaggccgc atctgctgag cagcggcatg tgtatgtggc
     1681 atgcagcaat tacgatgaca acctgcctca cttgcaatac ttggccgcgc aagccgattt
     1741 ggattaggcc acagcgaaaa tgatgttttg gtgtttgggt gggcgctatt tggcgcagtt
     1801 tgcgcccgaa catgccaaat accaaagcga agatgcgcag ttgtgccgtg aattaatcac
     1861 acgcttgcaa caaggctttt atcccgatgc caatatttat tttgaccctg agcaagatag
     1921 ccctgtgcgc tccagcgaat acagcgattg ccctgatgtg tggcagatac ccgccatgat
     1981 gtacgctgtg gtgatgggca gcgagtatgt ggatttggat gatgatgctt atgatgatgg
     2041 tttgcctttg actgtggcta ccgccgtgtg gaatgccgat tacgattgag taataaaaca
     2101 gataagcggc ctgtaaatgg cggcaccaga taaaaccata tggtaagcat accgtaagca
     2161 aacaaggcaa tctgcctcaa agcgattgcc tgtttttaaa aatatatatt tctcttctaa
     2221 cagaaatttc ggaattatta tgaacatcct tttactcggc agtacaggtt tgattggttc
     2281 agcagtacac gccaagttga gcgcaacaac tcacactgtt attgcaccga catcggcgga
     2341 attggatttt cagcgccctg acacatgggc ggcgtggttg caacaagccg atgtgctggt
     2401 caattgcgtg ggcgtgatgt cggcagacag cggcttgatg cagagcgtgc aatgcactgc
     2461 gccgcaagcg ttgtaccagc aagcccaacg agcagggatg ggctatgtgt tgcaagtatc
     2521 ggctttggga gcatcggttg atgctgacac ggtgttttta cgcagcaaag gccagttgga
     2581 tgcgtggttg ctggcaagct gcctgaacgt gggcatcgtg cgccccagtt tggtgtttgc
     2641 caaaacaggg cgcagcagtc gcttgtttgt gaacttggcc aagctgccac acctgtggct
     2701 gccagaggca gggcacatgg tgatacagcc tttgaccata caacctgtgg ccttgaacga
     2761 tgtggcaaca gcagtggtgg cgatgttgca aagccgttat tcaggcgtgg tgaatgcggt
     2821 cgggggcaag gcgctgagtt tggcggccta cctgactttg ctgcgacaat acgcttggca
     2881 acgagggcct gcacacatac acgccttgcc tgtgacttgg gcgaagcgtg cagcggaggt
     2941 gttgcaacat gtcagctcag gtttgatcac gcctgccagc atagagatgt tgcttgagag
     3001 tgaagaagtc gaagtggaag atttcgcacg aatattgggc aggcagccac aaacacctgc
     3061 acagttttta caggccgctt atgcacaagg agcacacaaa tgaacatgcg caaagtcatg
     3121 gtggctgcct tggtggtgat gtggctgtac agcggtttgc aaccgatatt gaccgtgccg
     3181 accgagtcgc tgcaaatgtt ggcgcgagta ggggttagtg cagcatggca gtggccgctg
     3241 ctgctcgcgg catcggtgtg cgatgtggcg gtggccttgt ggatagtgtg gcgacccagt
     3301 cgcacgctgt ggtggctgca atgtggtttg gtggcgtttt acagtgtggt gatcgcgctg
     3361 tggctgcccg aaaactggct gcatccattt ggcccattga ttaaaaacct gcccatcatg
     3421 gcgatgatgg cgcatttggc aacaactgaa gcaaggaagg tgtgagatgg aatactattt
     3481 atgggtaaaa gccctgcaca tattgtcgtc tgtgttgatg gtgggcacag ggtttggcac
     3541 tgcgttttac ctgttttggg ccaaccgcag tggctc
//
LOCUS       DAOMVK010000214         3571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_7617_length_3571_cov_4.731229, whole genome shotgun sequence.
ACCESSION   DAOMVK010000214 DAOMVK010000000
VERSION     DAOMVK010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7617_length_3571_cov_4.731229"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1394)
                     /gene="nuoL"
                     /locus_tag="PK856_07540"
     CDS             complement(<1..1394)
                     /gene="nuoL"
                     /locus_tag="PK856_07540"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRL02258.1"
                     /translation="MDKMNLYLVIALAPLVGSLLAGLLGKVIGRKGAHIVTILGVAIS
                     FVLSCGVLYDFWTGKGQVFDQNIYTWLTMGGLDFGIGFMVDSLTALMLVVVTSVSLMV
                     HIYTIGYMSDDNGYQRFFSYISLFTFSMLMLVMSNNFVQLFFGWEAVGLVSYLLIGFY
                     FQRDTAIFANLKAFLVNRVGDFGFVLGIALVLAHFGGSLNYADVFAAAPGLKDATIQL
                     FPGVDWSLLTVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGIF
                     MVSRMSPLYEMSTVALNVIVVIGAITALFMGFLGLIQTDIKRVVAYSTLSQLGYMTVA
                     LGVSAYSIAMFHVMTHAFFKALLFLAAGSVIIGMHHDQDMRNMGGLRKYMPITHITML
                     LGSLALIGTPFFSGFYSKDSIIEAVHASQLPAADFAYVALIISVFVTALYCFRQYFMV
                     FWGKERWREHKGHD"
     gene            complement(1421..1726)
                     /gene="nuoK"
                     /locus_tag="PK856_07545"
     CDS             complement(1421..1726)
                     /gene="nuoK"
                     /locus_tag="PK856_07545"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HRL02259.1"
                     /translation="MISITHYLILASLLFGVSAMGIFMNRKNVLVILMSIELMLLAVN
                     FNFVAFSQYLGDTAGQIFVFFILTVAAAEAAIGLAILVLIYRNRQSINVQDLDSLKG"
     gene            complement(1723..2373)
                     /locus_tag="PK856_07550"
     CDS             complement(1723..2373)
                     /locus_tag="PK856_07550"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRL02260.1"
                     /translation="MMFQTILFYVFAAIVLFGAVQTVTAKSPVHAALYLVLTFCTSAL
                     MWMLMQAEFLGIVLVLVYVGAVMVLFLFVVMMLNIDVEEMRAGFWKNLPVSGTVGVLM
                     AVVLILALIDPKTNLAAFGAMADVPADYSNVRALGREIYTTYLLPFELAAVLLVLGMV
                     AAIALTHRGNKNPRYIDPAEQVKVQAKDRFRMVKIQPEVEVVEAAAEGETKQEVKS"
     gene            complement(2380..2859)
                     /gene="nuoI"
                     /locus_tag="PK856_07555"
     CDS             complement(2380..2859)
                     /gene="nuoI"
                     /locus_tag="PK856_07555"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HRL02261.1"
                     /translation="MANLVKSFLLTELLKGMSITLKNFFSPKLTLMFPEETTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAISIESEQREDGTRRTTQYDIDLTKCIFC
                     GFCEEACPVDAIVETHILQYHGEKRGDLYYTKPMLLAVGDRYEDEIAKSKAADAPYR"
     gene            complement(2879..>3571)
                     /locus_tag="PK856_07560"
     CDS             complement(2879..>3571)
                     /locus_tag="PK856_07560"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HRL02262.1"
                     /translation="ASLLYIMAITSFGVYGVIIAGWASNSKYAFLGAMRSSAQIISYE
                     IAMGFALVGVIMVSGSLNFVDIVNQQGQGIAGGSVLSWNWLSLFPLFVVYLISGVAET
                     NRAPFDVAEGESEIVAGFHVEYSGMAFALFFLAEYINMILIGALTALLFLGGWLSPFP
                     ASWGIIGAASPVWMFIKMAMVLYCFLWFRATFPRYRYDQIMRLGWKVFIPVTLVCVAL
                     LGLWMISPWSLW"
BASE COUNT         1184 a          908 c          710 g          769 t
ORIGIN      
        1 tggtcgtgac ccttgtgttc acgccaacgc tctttgcccc agaacaccat gaaatactgg
       61 cggaagcaat acaaagccgt tacgaataca gagatgatca aagccacata agcaaaatct
      121 gctgctggca attgtgaggc atgcactgct tcaataatgg agtcttttga atagaaacct
      181 gagaagaacg gtgtaccaat caaagccaaa gaacccaaca acatggtgat atgggtaatc
      241 ggcatgtatt tacgcaagcc acccatattg cgcatgtctt gatcatggtg cataccgatg
      301 atgaccgaac ctgcggctaa gaacaacaag gctttaaaga aagcatgagt catcacatgg
      361 aacatggcaa ttgagtaagc agacacaccc aaagccacag tcatgtaacc caattgtgac
      421 aaggtagagt acgcaaccac acgtttgata tcggtttgaa tcaagcccaa gaaacccatg
      481 aacaaagcag taatggcacc aatgactacg attacattca atgcgacagt cgacatttca
      541 tacaatggcg acatgcgtga caccatgaag atacctgcag tcaccatggt ggccgcgtgt
      601 atcaaggctg aaatcggtgt agggccttcc atagaatctg gcaaccacac atgcaatggg
      661 aactgagccg acttacccat cgcgcccaca aacaacagca agcaagtcac agtcaacaaa
      721 gaccagtcca cgccagggaa caattggatg gtggcatctt tcaagccagg cgcagcagca
      781 aacacatcgg cgtagttcaa actaccaccg aaatgagcca gcaccaaagc gatacccaat
      841 acaaagccaa agtcaccaac acggttaacc aagaatgctt ttaagttggc gaaaatagcg
      901 gtgtcacgtt ggaagtaaaa accaatcaac aaataagaca ccaaacccac cgcttcccaa
      961 ccgaagaaca actgtacaaa gttgttgctc atcaccaaca tcaacattga gaaagtaaac
     1021 aatgagatgt aagagaagaa acgttggtag ccattgtcat cgctcatata gccgatggta
     1081 taaatgtgca ccattaaaga cacactggtc accaccacca acattaaagc agttaagctg
     1141 tcgaccatga agcctatgcc aaaatccaaa ccacccatgg tgagccatgt gtagatgttt
     1201 tggtcaaaga cttgtccttt gccagtccag aagtcgtaca agacgccaca agacagcaca
     1261 aaagaaatgg ccacacccaa aatcgtcacg atatgggcac ctttacggcc gattacctta
     1321 cccaacaaac cggccagcaa ggagccgacc agtggggcaa gtgcaatcac taaatacaaa
     1381 ttcattttat ccataatgat tgatttcttc ttgggtgaaa ttagcctttc aggctgtcta
     1441 aatcttgtac gttaatgctt tgacggttac ggtaaatcaa taccaaaatc gccaaaccga
     1501 tggcagcttc tgctgctgct accgtcaaaa tgaaaaacac aaagatttgt ccggcggtat
     1561 cgcctaaata ctgagaaaaa gccacgaagt taaaattcac cgccaataac atcaattcaa
     1621 ttgacatcaa aatgaccaag acattcttac ggttcataaa aatgcccatg gcactgacac
     1681 cgaataacaa agaggccaaa atgagatagt gggtaatcga aatcatgatt tcacctcttg
     1741 tttggtttcg ccttcagcag cagcttctac cacttctact tcaggttgaa ttttcaccat
     1801 gcggaaacgg tctttggctt gcactttaac ttgctcggct ggatcgatgt aacgtgggtt
     1861 tttattgcca cggtgggtca aggcaatggc cgcaaccatg cccaacacca acaacaccgc
     1921 tgccaattca aacggcaaca agtaagtggt gtaaatttca cgacccaaag cacgcacatt
     1981 gctgtaatca gcaggcacat cagccattgc accgaaagca gccaaattgg ttttgggatc
     2041 aatcaaagcc aaaatcaaca ccactgccat caaaacgccg acagtgcctg atacaggcaa
     2101 gtttttccaa aatccagcac gcatttcttc aacatcgatg ttcagcatca tcaccacgaa
     2161 caagaacaac accatgaccg caccgacata caccaacacc aacacaatgc ccaggaattc
     2221 agcttgcatt aacatccaca tcaaagcact ggtacaaaac gtcaatacca agtacaaagc
     2281 ggcatgtaca gggcttttgg cggtaacggt ttgaaccgca ccaaaaagca ctattgctgc
     2341 aaatacataa aataagatgg tttgaaacat gatcttccct tatcgatatg gcgcgtcagc
     2401 tgctttgctt ttggcaattt cgtcttcgta acgatcgcca actgccaaca acatcggttt
     2461 ggtgtaatac aaatcaccac gtttttcacc gtgatactga agaatatgtg tttccacaat
     2521 cgcatctact gggcaagctt cttcacagaa accgcagaaa atacatttgg tcaaatcgat
     2581 gtcgtattgc gtggtgcgac gggtaccgtc ttcacgttgc tcagattcaa tcgaaatcgc
     2641 cattgcagga caaactgctt cgcacaattt acacgcgata caacgctctt caccattagg
     2701 gtaacgacgc tgcgcatgca aaccacggaa acgcactgat tgaggcgtgg tttcttcagg
     2761 gaacatcaac gtcaattttg gtgaaaagaa gtttttcaac gtaatcgaca tgcccttgag
     2821 aagctcagtc aataaaaatg attttactaa attagccatt gttttactct cttattcctt
     2881 accacagtga ccatggagaa atcatccaca agcctagtaa agctacacat accaaggtta
     2941 caggaatgaa tactttccaa cccaaacgca tgatttggtc ataacggtaa cgtgggaagg
     3001 ttgcacggaa ccataagaag caatacaaca ccattgccat cttgatgaac atccaaactg
     3061 gactggctgc accaataatg ccccagcttg ctgggaatgg agacaaccaa ccacccaaga
     3121 acaacaacgc agtcaatgca ccaatcaaaa tcatgttgat gtattcggcc aagaagaaca
     3181 aggcaaatgc cataccagaa tactcaacgt ggaaaccagc tacgatttca gattcaccct
     3241 cagccacgtc aaatggcgca cggttggttt cggccacgcc tgaaatcaag tacaccacaa
     3301 acaatgggaa caaagacaac cagttccatg acaagacaga accacctgca atgccctgcc
     3361 cttgttgatt cacgatgtcc acgaaattca aactacctga aaccatgatg acgccaacca
     3421 aagcaaaacc catggcgatt tcgtaagaga tgatttgcgc agaagaacgc atggcaccca
     3481 aaaacgcgta ttttgagtta gacgcccaac ctgcaatgat cacgccgtac acaccaaaag
     3541 aggtgatggc catgatgtaa agcaagcttg c
//
LOCUS       DAOMVK010000215         3521 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_7758_length_3521_cov_3.310444, whole genome shotgun sequence.
ACCESSION   DAOMVK010000215 DAOMVK010000000
VERSION     DAOMVK010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7758_length_3521_cov_3.310444"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PK856_07565"
     CDS             complement(<1..244)
                     /locus_tag="PK856_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02263.1"
                     /translation="MSKNILLATWNEADTAYRVFSELTQAGIAGVNQVAIIAKTENGK
                     IKLKDADSNTIGKSTVKGGLIGMVVGVLGGPVGVLLG"
     gene            418..969
                     /locus_tag="PK856_07570"
     CDS             418..969
                     /locus_tag="PK856_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02264.1"
                     /translation="MRFLTLLLLFGGSYAHADTLFIHENPNKSGHCYMTNSAGKKVFE
                     GEYQTDCFAIGTSQLSTDPNADEPATGYHTVHLFQFRGQTGVAINPKGEVVYHTYWFD
                     NGPDYVEDGMFRMRDAHGKIGYADGFTGRMVIAPQYACAFPFENGVAQVALRGEVVRE
                     DEEHSVCVGDYFKIDKQGKRVAE"
     gene            1041..2145
                     /gene="prfB"
                     /locus_tag="PK856_07575"
     CDS             join(1041..1121,1123..2145)
                     /gene="prfB"
                     /locus_tag="PK856_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HRL02265.1"
                     /translation="MEAEIINQLNNTLDDLEQRNQDIRGYLDYDGKKDRLEEVVGLSE
                     DPELWNDPKKAQEIGKERKLLEGIVVNLDEISSGIDDSRMLLEMAVEEADLEAFTGVQ
                     EDIAVLEKRMADLEFKRMFNQEADSNNCFIDIVAGAGGTEAEDWAGMLYRMYTRYAER
                     KGFKVDILEEDFGEIAGINRATIKLEGDYAYGLLRTETGIHRLVRYSPFDSNNKRHTS
                     FASVFVYPEVDDSFEIEINPSDLRVDTYRASGAGGQHINKTDSAVRLTHEPTGIVVQC
                     QNDRSQHRNKDEAMNMLRAKLFELEMRKRNEAKQDLENSKSDVGWGHQIRSYVFDQSR
                     IKDLRTGYEVGNIKAVMDGDLDDFIQASLKQGV"
     gene            complement(2240..2725)
                     /locus_tag="PK856_07580"
     CDS             complement(2240..2725)
                     /locus_tag="PK856_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02266.1"
                     /translation="MNTSPQMLFLQANAFFYDGVTAQKTYWQTYASAPDKMPFLLMHV
                     CDGHQDLAEATHGFLNTVYWMACVYHTYCGGFCDATEREDLVIQAQQAQMYLHGFIDG
                     LLNEAYTNVELEAYLHAHFDHSLFEAVYAAMGYEAMQIDIDAAINVWDFATHPDVQQY
                     L"
     gene            2943..>3521
                     /locus_tag="PK856_07585"
     CDS             2943..>3521
                     /locus_tag="PK856_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HRL02267.1"
                     /translation="MGTNRLEAFSDGVLAIIITIMVLELKVPEGMDWAALRPGLPIAL
                     SYVLSFVYVGIYWNNHHHLFQLAQKASGSVLWANLFMLFWLSLFPVTTAWLGEHPHAS
                     APSMLYGVVLLMAALGFYMLERRIIAVDPNNQALKTALGRNLKGKLSPLCYVLGIVLA
                     YFSPLLGQLIYALMAALWLIPDRGMEQAAQSHP"
BASE COUNT          908 a          904 c          851 g          858 t
ORIGIN      
        1 agcccaacaa caccccaaca ggcccaccca atacgcccac caccatgcca atcaaaccgc
       61 ctttgacagt ggatttgcct atggtattgc tgtcagcatc tttgagcttg attttgccat
      121 tctcggtttt ggcaatgatg gccacttggt tgacgcctgc tatgcctgct tgggtcaatt
      181 cgctgaacac acggtaggcc gtatccgcct cattccatgt cgccagtaaa atgtttttac
      241 tcatgcctta ccctccatta atgcttggtt tatgattgga aacagcgatt tcggcatctt
      301 accctagggc tgaggtttct cgctagcatg cccaagcgaa atttaccgtc ccagtgccac
      361 agcttcagcc gtttttgcgc cagctcaatt caaccctctt tttacaggct gcttaccatg
      421 cgatttttga ctctgttatt gctgtttggc gggtcttatg cccatgcaga caccttgttc
      481 atccatgaaa accccaacaa atcggggcat tgctacatga ccaattcggc aggcaaaaaa
      541 gtgtttgagg gagaatacca aaccgattgt ttcgccatag ggacgagtca actcagtaca
      601 gaccccaatg ccgatgagcc tgcaacaggc taccataccg tgcatttgtt tcagtttcgt
      661 ggccaaacag gtgtggccat caaccccaaa ggcgaggtgg tttaccacac gtattggttt
      721 gataacggcc ctgattatgt cgaagacgga atgtttcgca tgcgcgatgc ccatggcaag
      781 attggttatg ccgatggttt tacaggccgc atggtgattg cgccgcaata cgcttgcgct
      841 tttccctttg aaaacggcgt ggcccaagtg gctttgcgcg gcgaagtggt gcgtgaagac
      901 gaggaacaca gcgtttgtgt cggcgattat tttaaaattg ataagcaagg taaacgcgtc
      961 gccgagtgat taggcgagtt tgccgtttta cagtacaata accgccttta tattgaaccc
     1021 gttacacgaa agaaaacaac atggaagcgg aaatcatcaa ccaattgaac aacactctcg
     1081 atgacttgga gcagcgcaac caagacatca gggggtatct ttgactacga tggcaaaaaa
     1141 gaccgtctag aagaagtggt gggcctctct gaagacccag aattgtggaa cgacccgaaa
     1201 aaagcccaag aaatcggcaa agaacgcaaa ttgcttgaag gcatcgtggt gaatttggac
     1261 gaaatcagct caggcataga cgacagccgc atgttgttgg aaatggccgt agaagaagcc
     1321 gatttggaag cgtttacagg cgtgcaagaa gacatcgccg tgttggaaaa acgcatggcc
     1381 gatttggaat tcaagcgcat gttcaaccaa gaagccgaca gcaataactg cttcatcgac
     1441 atcgtggctg gtgcaggcgg cacggaagca gaagactggg cgggcatgct gtatcgcatg
     1501 tacacccgct atgccgaacg caaaggcttt aaagtcgaca tcttggaaga agactttggt
     1561 gaaatcgcgg gcatcaaccg cgccaccatc aagctagaag gcgactatgc ctatggcttg
     1621 ctgcgcaccg aaacaggcat ccatcgcttg gtacgctact cgcctttcga ttccaataac
     1681 aaacgccaca cctcgtttgc ctctgtgttt gtatacccag aagtggacga ttcgtttgaa
     1741 atcgaaatca acccctctga tttgcgtgtc gacacctacc gcgcttcggg tgccggtggt
     1801 cagcacatta acaaaaccga ctcggccgtg cgcttgaccc acgaacccac aggcatcgtg
     1861 gtgcagtgtc aaaacgaccg ctcgcaacac cgcaacaaag acgaagcaat gaacatgttg
     1921 cgcgcgaaac tgtttgaatt ggaaatgcgc aaacgaaacg aagccaaaca agacttggaa
     1981 aacagcaaat ccgatgtggg ttggggccac caaatccgct cgtatgtatt cgaccagtcg
     2041 cgcatcaaag atttgcgcac aggctatgaa gtgggcaaca tcaaagcggt gatggacggt
     2101 gacttagacg acttcattca agcgagcttg aagcaaggcg tgtaaaccct ctgcgtttca
     2161 tgtgaaacca atcaaaatcg gcctgtaaac cttttacagg ccgattcgtc atgcatctct
     2221 ttgtattttc aacacatgct cacaagtatt gttgcacatc aggatgagta gcgaaatccc
     2281 acacattgat tgcggcatct atgtctattt gcatggcttc atagcccata gccgcgtaca
     2341 ccgcttcaaa caaggagtgg tcaaaatgtg cgtgtaaata cgcctccagc tcaacattgg
     2401 tataagcctc gttaagcaaa ccgtcgatga acccatgcaa atacatctga gcttgctgcg
     2461 cctgtatgac caaatcttct cgttcagtag catcgcaaaa gccgccacaa tatgtgtggt
     2521 acacgcaagc catccaatac acggtattca aaaaaccatg ggtggcttca gcaaggtctt
     2581 gatgcccatc acacacatgc atcaataaaa acggcatttt gtcaggcgca gaggcatagg
     2641 tttgccaata tgttttctgc gccgtcacgc cgtcataaaa aaacgcattg gcttgtaaaa
     2701 acagcatctg tgggctggtg ttcatccgta caacctttcc tagtgtgatg gtagaccaag
     2761 cggcctgtaa gccttacagg ccgcttatac cgcttgtttt atcaacaagc cacattctga
     2821 gcccatccct ccgcaccccg ccaccaatat ttttagccaa tttgtgtggg cacagtaaac
     2881 caattgcttc agcccccact cttaatgctc tgttcgtgaa gtgaatactc aagggggcta
     2941 ttgtgggaac caaccgttta gaagcattca gtgatggcgt gctcgccatc atcatcacca
     3001 tcatggtgtt ggagttaaaa gtgcctgagg gcatggattg ggccgcgctg cgtcctggcc
     3061 tgccgattgc actgagttat gtgctcagct ttgtatatgt gggcatttac tggaacaacc
     3121 accaccacct gtttcaactg gcacaaaaag cctcaggctc ggtgttgtgg gccaatttgt
     3181 ttatgctgtt ttggctatct ttattccccg tgaccacggc gtggttgggt gaacatccgc
     3241 acgccagcgc accgagcatg ttgtatggcg tagtgttgct gatggcggct ttgggttttt
     3301 acatgttaga gcggcgcatc atcgccgtag accccaataa ccaagccctc aaaaccgcat
     3361 tggggcgcaa tctcaaaggc aaattgtccc cgttgtgtta tgtgctgggc atcgttttgg
     3421 cctatttttc accattatta gggcaattga tatacgccct catggcggcg ttgtggctga
     3481 tacccgaccg tggcatggag caagcggctc aatcccatcc t
//
LOCUS       DAOMVK010000216         3518 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_7777_length_3518_cov_2.518048, whole genome shotgun sequence.
ACCESSION   DAOMVK010000216 DAOMVK010000000
VERSION     DAOMVK010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7777_length_3518_cov_2.518048"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PK856_07590"
     CDS             <1..295
                     /locus_tag="PK856_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HRL02268.1"
                     /translation="VLMHGDKHISTRQLARDLGMKHIEAADPKQANKWTGYLVGGTSP
                     FGTKTVLPVYVEASILELDTIYINGGKRGFLVVMQAADLQVLQPQLVNVAVDA"
     gene            439..1632
                     /locus_tag="PK856_07595"
     CDS             439..1632
                     /locus_tag="PK856_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRL02269.1"
                     /translation="MNKKIKWLLLTMVVLAIGLAAYFFWFNKQDKVDYITETVARGDL
                     AMTVNATGTVAAAQKVTVGAQASGQIKKLYIKVGDEVKKGDLIAQIDPITQQNTLQTK
                     QAELASLNAQLANQKIQLDIASKQYQREQALLAEDASSREAVENAKDSWSAAQAALKQ
                     TQASIKQTTVLINTAEVELGYTKIIAPFTGTVVSVPVEEGQTVNANQTTPTIAEIADL
                     SQMEVKLEIAEGDITKVKAGLPVEYTILSEPNEKFHTTLQSIDPGLTTLTDGTYSEST
                     KSEAAVYYYAKLLVPNEAGKLRIGMTTQNVITSKEVKNTVIVPTMAIRNQKGKRIVRV
                     LQADGKVEEREIKVGLTDGLNTQAVSGLKEGEKVIMGSMNASEKSQAVQQGQGRRPSG
                     GMGPR"
     gene            1638..2739
                     /locus_tag="PK856_07600"
                     /pseudo
     CDS             1638..2739
                     /locus_tag="PK856_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     assembly_gap    2740..2749
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2750..>3518
                     /locus_tag="PK856_07605"
     CDS             <2750..>3518
                     /locus_tag="PK856_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRL02270.1"
                     /translation="QYFDVNGKKLAAGRFFTEDDVKNNESVVVIDPNTVKQLFGEGER
                     PLGKVILFNNVPLKVIGITEADTGFGANENLNMWSPYTTVMNKITGARNISSVTVKVK
                     DNVSSQVAEQSITALLEARHGTKDFFTRNSDSIKQTIESTTGTMTMLISSIALISLIV
                     GGIGVMNIMLVSVTERTKEIGVRMAIGARQMNILSQFLIEAVLICLLGGFIGIALSGL
                     IGLIFNQFVSSFPMSFSTGSIVLALTCSTLIGVAFGY"
BASE COUNT          950 a          809 c          939 g          810 t
ORIGIN      
        1 ggtattgatg catggggaca agcacatttc cacccgccaa ttggcccgtg atttgggcat
       61 gaaacacata gaggcagccg accccaagca agcgaacaaa tggacgggtt atttggtggg
      121 cggcaccagt ccgtttggca ccaaaacggt tttgcctgtg tatgttgagg ccagtattct
      181 ggaattggac accatttata tcaatggcgg taaacgtgga ttcttagtgg tgatgcaagc
      241 ggcggatttg caggtcttgc agccacaact ggtgaatgtg gcggtggatg catgatgaaa
      301 atgcaaataa tttgcatgtg ctaagtttct tctaagtttc aggccgcata atgtcagtca
      361 attcaggtag acttggccga gcattgcgct tggcgctgca ctacacagca atcatcattc
      421 aaatgggttt attcgcgcat gaataaaaaa atcaaatggc tactgttgac catggtggtc
      481 ttggcgatag gcttggcggc ttattttttc tggtttaaca aacaagacaa agtcgattac
      541 atcaccgaaa ccgtggcgcg tggcgatttg gccatgacgg tgaatgcaac gggaaccgtg
      601 gcagctgctc agaaggtgac ggttggtgcg caggcctcag gccaaatcaa aaaactctat
      661 attaaagtgg gtgatgaagt gaaaaaaggc gatttaatcg ctcaaatcga cccgattacc
      721 caacaaaaca ccttgcaaac caaacaagcc gaattggctt ctttgaatgc gcaattggcg
      781 aatcaaaaaa ttcagttaga cattgcttct aagcaatacc aacgtgaaca ggctttgttg
      841 gcagaagacg cgagctcacg cgaagcggtg gaaaatgcca aagacagttg gtcagcagca
      901 caagcggcct taaagcaaac ccaagcctcc atcaaacaaa ccacggtatt gattaacact
      961 gccgaagtgg aattgggcta taccaaaatc atcgcaccgt tcacaggtac ggtggtgtcg
     1021 gtgcctgtgg aagaaggcca aaccgtcaat gccaatcaaa ccacaccgac cattgctgaa
     1081 atcgctgatt tgagccaaat ggaagtgaag ttggaaattg ccgaaggcga cattaccaag
     1141 gtaaaagcag gcttgccagt ggagtacacc attctgtctg agccaaatga gaagtttcac
     1201 accactttgc aatccatcga tcctggcttg accactttaa ccgatggcac ttacagcgag
     1261 tcgaccaaaa gcgaggcagc ggtgtattac tacgccaaac tgttggtgcc caatgaggcg
     1321 ggaaaactgc gcataggcat gaccacacaa aacgtgatta cctcgaaaga agtgaaaaac
     1381 accgtgatag tgccgaccat ggcgatacgc aatcaaaaag gcaaacgcat cgtgcgggtg
     1441 ttacaggccg atggcaaagt agaagagcgt gaaatcaaag tcggcttgac cgatggcttg
     1501 aatacccaag cggtgtctgg cttgaaagag ggtgaaaaag tcatcatggg cagcatgaat
     1561 gccagtgaga agtcgcaagc ggtgcaacaa ggacaaggcc gtcgtcctag cggtggcatg
     1621 ggcccgaggt aagcaccatg gcgatgatcg aaattaaaaa cctgaacaag tattttggtg
     1681 aaggtgaaaa ccgcgtccac atcttaaaaa acattaatct atcggtggaa caaggcgatt
     1741 tcattgccat catcggccaa tcgggctcgg gcaaatccac actgatgaat gccattggct
     1801 gtttggatac gcctagctca ggctcttacc aagtcgatgg catcgaaacc ttaaacatgg
     1861 aagccgatga tttggcggcc ttgcgccgca aaaagtttgg cttcattttt caacgctaca
     1921 atttgctgtc cagcttgagc gcgcgtgaca acgtggcctt gcctgcggtg tatgcgggtg
     1981 tggatgaaaa actgcgccat gagcgcgcgg tgcaattgct cagcgatttg ggcttgggcg
     2041 acaagaccag caatttgccc agcgaattgt ctggcggtca gcaacagcgg gtgtctattg
     2101 ctcgcgcgct gatgaatggc ggcgaaatca tcttggccga cgagccaact ggggcgctcg
     2161 attcggcttc gggtgtgatg gtgatgggca ttttgcaaga cttgtatgcg caaggccaca
     2221 ccatagtgtt ggtaacccac gatgccaaaa tcgccgatta tgccaaccgc gtcatcgaaa
     2281 tcaaagacgg tgaaatcgtt tctgacgtga gcaaagaaac cgttaaagcg ccgatacagc
     2341 aagcaaaact caaagagaaa tcgacactcg gtttttacaa agaccaagtg ggcgagtcgt
     2401 tcaaaatggc ggtgcaggcg attgtggcgc acaaaatgcg ttctttgttg accatgcttg
     2461 gcatcatcat cggcattgcc tcggtggtgt cggtggtggc cttggggcgc ggttcacaag
     2521 agcagatatt gcagaatatt tcctccatag gcaccaacac catttcgata tacccaggct
     2581 cgggctttgg tgacagacgt tccaatcggg tcaaaacctt ggtggtggcc gatgctgaag
     2641 ccttggccga acaaacctat gttgacaacg tcacgcccag tgtctctacc agcggtttgt
     2701 tggtgtttgg cagcacgtct ttaaccggct ctttaacggn nnnnnnnnna ccagtacttt
     2761 gacgtgaacg gcaaaaaatt ggctgcaggc cgcttcttta ccgaagatga tgtgaaaaac
     2821 aatgaatcgg tggtggtcat agacccaaat acggtgaaac aattgtttgg tgagggtgag
     2881 cgcccattgg gcaaagtcat cttgttcaat aatgtgccgc ttaaagtgat tggcattaca
     2941 gaagccgata caggttttgg cgccaatgaa aacctcaata tgtggtcgcc ttacaccacg
     3001 gtgatgaaca aaatcacggg cgcacgcaac atcagctcgg tcacggtgaa ggtgaaagac
     3061 aatgtcagct cacaagtggc cgagcaaagc attaccgcct tgcttgaagc ccgccacggc
     3121 accaaagact tcttcacccg caacagcgat tcgatcaagc aaaccataga atccaccacg
     3181 ggcacgatga cgatgttgat ttccagcatt gccttgattt cgctgattgt gggcggcata
     3241 ggcgtgatga acatcatgtt ggtgtcggtg accgagcgca ccaaagaaat cggtgtgcgc
     3301 atggccattg gtgcgcggca aatgaacatc ttgtcgcagt ttttgataga ggcagtgttg
     3361 atatgcttgc taggtggctt cataggcatt gccttgtctg gcttaatcgg cttgattttc
     3421 aaccagtttg tcagcagctt tccgatgtcg ttttccacag gctcgattgt gttggccttg
     3481 acttgctcga ccttgattgg ggttgcgttt ggctacat
//
LOCUS       DAOMVK010000217         3513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_7796_length_3513_cov_2.707634, whole genome shotgun sequence.
ACCESSION   DAOMVK010000217 DAOMVK010000000
VERSION     DAOMVK010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7796_length_3513_cov_2.707634"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..642)
                     /locus_tag="PK856_07610"
     CDS             complement(142..642)
                     /locus_tag="PK856_07610"
                     /inference="COORDINATES: protein motif:HMM:NF022479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HRL02271.1"
                     /translation="MLLQMTTPALIFPAISLIMLAYTNRFLGLSSVIRSLTHEYQQHP
                     DEALLLQIDNLQRRVIMVRNMQLFGVLCIMFSIASIWALSVDNAFWGMGLFIACLVCM
                     AVSLLISMYELILSCHALEIALADMEKDLKQISHPILSRPRRRAKRASVKQERKEVQE
                     LEDNIK"
     gene            complement(690..1157)
                     /locus_tag="PK856_07615"
     CDS             complement(690..1157)
                     /locus_tag="PK856_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02272.1"
                     /translation="MSKRLYSTQTAWPWSRLQLCLYMYLCGVVLTAAFFLTYHFLFQI
                     NDAMWVFVILLMLTLFILFYVASLPFKKIKVVDFYAKHLEIRAWNFWNGRGREILSQI
                     AYSELVSVEQLVMQRVVLSLRFHQHPPLCLRFESRLYEFLQQELISCSAKPFD"
     gene            complement(1150..1317)
                     /locus_tag="PK856_07620"
     CDS             complement(1150..1317)
                     /locus_tag="PK856_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02273.1"
                     /translation="MFTLFVLALITIGVVAAVLIGMIRQSRAEIMALRRQVQRLEARF
                     DGIAAQEKMHE"
     gene            complement(1470..>1632)
                     /locus_tag="PK856_07625"
     CDS             complement(1470..>1632)
                     /locus_tag="PK856_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HRL02274.1"
                     /translation="AGKDLHVETVELDMYAAIDVLMDKLDRMVLKHKEKNTDYRTTPS
                     GRETIAETE"
     assembly_gap    1633..1642
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1643..1815)
                     /gene="raiA"
                     /locus_tag="PK856_07630"
     CDS             complement(<1643..1815)
                     /gene="raiA"
                     /locus_tag="PK856_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HRL02275.1"
                     /translation="MNLTVTGLNLDVTEALREYVSTKLARISRHADNIISTTVTLSTV
                     EKGQHKVEADVHLA"
     gene            complement(1926..3350)
                     /gene="rpoN"
                     /locus_tag="PK856_07635"
     CDS             complement(1926..3350)
                     /gene="rpoN"
                     /locus_tag="PK856_07635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HRL02276.1"
                     /translation="MQNQLKLKQTQQLQLNPQLQQAIKLLQMSGLELDEAVEQALLEN
                     PLLERVESDFSEESHEPEVLSSEAESMEFDANGDFEEDWQSLQLTAGLGSTGITYEGE
                     FQDAFAQIEDNSDFRQFLRDQVCEHQLSDAEVFSVRVLIEHLDEHGYLSTSLTEVVEH
                     SPLEWFLEEEQLAAALVKLQRFEPAGVGATDLAESLLLQLQRMAEDETIVLAKLIVRQ
                     YFNVLAQPSNVTKLKKYLPMSAAEIQTAVMLIQTLNPYPSSGYAHAEPTQYVLPDIRV
                     FKKSGKWIAQLQRQSQPQIRVNEEYAAVVEESGHSDLKRQIQEAKQLISGLEMRSDTV
                     LRVADYVVEHQQEFFSQGAIALKPMLIKSVAEFLDLHESTISRAVNQKYLSCPQGLFE
                     LRWFFTQTATNSDGDGEDGISNRVIKTILEKLIGEEDKRKPLSDEKLAQKLLEHDIKI
                     ARRTVTKYRESLRIASASERKIRS"
     gene            complement(3364..>3513)
                     /locus_tag="PK856_07640"
     CDS             complement(3364..>3513)
                     /locus_tag="PK856_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004286233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter ATP-binding
                     protein"
                     /protein_id="HRL02277.1"
                     /translation="TDHNVRETLRICDRAYIISEGSVLATGLPEELVQNERVREVYLG
                     ENFDY"
BASE COUNT          963 a          879 c          693 g          968 t
ORIGIN      
        1 acggagaacc ttgatgaggc gcttagccca gagtgaacca tgtgtgtatt aaggtattca
       61 tgcaataggc atcaataaag cggcctgtaa accttacagg ccgcttaaat attgacaccc
      121 aaccacatcg gcttgtatct gctatttgat attgtcttcc aattcttgta cttccttgcg
      181 ctcttgcttg accgaggcac gtttggccct gcggcgaggt cgagacaaaa ttgggtggga
      241 aatctgtttc aaatcttttt ccatgtcggc caaggcaatt tccaaagcat gacaagacag
      301 aatcaactca tacatggaaa tcagcaaaga caccgccatg cacaccaaac aggcaatgaa
      361 caaacccatg ccccaaaatg cattgtctac cgacaaggcc caaatactgg caatgctgaa
      421 catgatgcag agcaccccaa ataactgcat gttgcgcacc atgatgacgc gccgctgcag
      481 attgtcaatt tgcaatagca aggcctcatc tgggtgttgt tgatactcgt gagtcaaact
      541 gcgtatcacg ctggacagcc ccaagaagcg gttggtatag gccaacataa tcaaggaaat
      601 ggctggaaaa atcagcgcag gcgtggtcat ttgtaaaagc attgggattc tctgaatgtg
      661 gtttgcttgg tgttcaaaat aaaaaatatt taatcaaatg gcttggcaga acatgaaatc
      721 aattcttgct gcaaaaattc atacagtcgg gactcaaatc tcaaacataa tggcggatgt
      781 tggtgaaaac gcaagcttag caccactctt tgcattacaa gttgctccac cgaaaccagt
      841 tccgaatacg ctatttggct caatatttcc cgccctcgcc cattccaaaa attccaagcc
      901 ctgatttcca aatgtttggc ataaaaatcc actactttga tttttttaaa tggcaatgaa
      961 gccacataaa acaatatgaa caatgtcaac atcagcaata tcacaaatac ccacattgca
     1021 tcatttatct gaaacaaaaa atggtaggtc agaaaaaaag cagcggttaa aaccacgcca
     1081 caaagataca tgtacaagca aagttgtaaa cgcgaccaag gccaagctgt ttgcgtcgaa
     1141 tacagacgct tactcatgca ttttttcttg tgctgcaatg ccatcaaaac gggcttccag
     1201 tcgctgaact tggcgcctca aggccatgat ttcggctcga ctctgtctga tcatgccgat
     1261 taaaaccgcc gccacgactc cgatggtgat caaggccaac acaaataagg taaacatttt
     1321 ctatttcctg ctcaatgggt tctattgtgc attgcacaca agaagcatac ctgaaattcg
     1381 tcgttaacaa tactgcgctc attgcagaac aagcggcctg aaatgacaaa agccatatct
     1441 ctcgatatgg ctgttttcat ttcaaccgat cattcggttt cagctatggt ttcgcgtccg
     1501 cttggagttg tcctgtaatc agtgttcttc tctttgtgtt taagcaccat tcgatcaagt
     1561 ttgtccatca acacatctat ggctgcgtac atgtccagtt ccactgtttc cacatgcaaa
     1621 tccttaccag ccnnnnnnnn nngccaaatg cacatctgcc tctactttgt gctgtccttt
     1681 ttccactgtg gacaaggtca ctgtggtaga aataatgtta tctgcatggc ggctgatgcg
     1741 agccaatttg gtgctaacat attcacgcaa agcttcagtg acatctaaat tcaaaccagt
     1801 tacagtcaga ttcatacaag tctccttctt ggcgggctat gcccaaattc ccatcatctc
     1861 gtgtgtgatg atgttgaatt catcatatcc tgtttgcacc aaaacgccaa tggctttatg
     1921 caagtttagc tgcgaatttt acgttcactt gcagaggcaa tgcgcaaact ttcacggtat
     1981 ttcgtcactg tgcgacgggc tatcttaatg tcatgttcca agagcttttg cgccaatttt
     2041 tcatccgaca gcggtttgcg tttgtcttct tcaccaatca gcttttccaa gatggtttta
     2101 atcacccgat tgctgatgcc gtcttctcca tcaccatcgc tgttggtggc cgtttgggta
     2161 aagaaccaac gcaattcaaa cagtccctga gggcaactga ggtatttttg attcactgca
     2221 cgtgaaatgg tggattcatg caaatccaaa aattctgcaa ccgatttaat cagcatgggc
     2281 tttaaggcaa tcgcaccttg tgagaaaaac tcttgctggt gctcaaccac ataatcggcc
     2341 actcgcaaca cagtgtccga acgcatttcc aatccactga tcaattgctt ggcttcttga
     2401 atctgtcttt tcaaatcact gtgaccagac tcttcaacca cagctgcgta ttcttcatta
     2461 acacgtattt gcggctgact ctgcctttgt aattgcgcaa tccatttgcc actttttttg
     2521 aatacccgta tgtcaggcaa aacatattgc gtaggctctg catgggcata accactgctg
     2581 ggatagggat tcaaggtttg gattaacatc actgcggttt gaatttctgc cgcgctcatg
     2641 ggcaagtatt ttttgagttt ggtcacgttg ctaggctgtg ccaaaacatt gaaatactgg
     2701 cgcacaatca atttggccaa cacgatggtt tcatcttcag ccatgcgctg cagttgcaac
     2761 aacaaagact cggccaaatc agtggcaccc actcctgcag gttcaaaccg ttgcaacttc
     2821 accaaagcgg cggccaattg ctcttcttct aaaaaccatt ccaatggtga atgttcaaca
     2881 acttcagtca agctggtcga caaataaccg tgttcatcca aatgctcaat caacacccgt
     2941 acagaaaaca cttctgcatc acttaattgg tgctcacaca cttggtcacg caaaaactga
     3001 cggaaatcac tgttgtcttc aatttgcgca aaagcgtcct gaaactcgcc ttcataggtt
     3061 atgcctgttg agcccaatcc tgctgtcaac tgcaaacttt gccagtcttc ctcaaaatcg
     3121 ccattggcat caaactccat gctttcggct tcgctggaca atacttcagg ctcatggctt
     3181 tcctcagaga aatcactctc cacccgctct aacaatgggt tttctaacaa agcctgctca
     3241 accgcctcat ccaactccaa accagacatc tgcaacagtt taattgcctg ttgcagttga
     3301 gggtttaatt gcagctgttg cgtctgcttg agtttgagtt ggttttgcat gtttcaatat
     3361 caatcaataa tcgaaatttt cgcccaaata cacttcacgg acacgctcat tctgcaccag
     3421 ctcttcaggc agacctgttg ccaatacgct gccttcggaa atgatgtaag cacggtcaca
     3481 aatgcgcaag gtttcgcgca cattgtgatc ggt
//
LOCUS       DAOMVK010000218         3503 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_7822_length_3503_cov_3.826856, whole genome shotgun sequence.
ACCESSION   DAOMVK010000218 DAOMVK010000000
VERSION     DAOMVK010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7822_length_3503_cov_3.826856"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /gene="rbsC"
                     /locus_tag="PK856_07645"
     CDS             complement(<1..283)
                     /gene="rbsC"
                     /locus_tag="PK856_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959255.1"
                     /note="functions to transport ribose at high affinity;
                     forms a complex with RbsA2C2B; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HRL02278.1"
                     /translation="MSSTNTKALLSRLGPFFGLLLLIIVITAMNPSFLTASNILNVLR
                     QVSISALIAFGMTFVILTRGIDLSVGSTLALTGAVAATLLASGTDPILAM"
     gene            complement(270..1763)
                     /gene="gguA"
                     /locus_tag="PK856_07650"
     CDS             complement(270..1763)
                     /gene="gguA"
                     /locus_tag="PK856_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRL02279.1"
                     /translation="MIQMSGISKSFSGNQVLNDVSFELANGEIHALMGENGAGKSTLM
                     KILSGIHHKDSGHILVDGEEQTFKTIKDSERSGIHVIHQELNILPDLSVAENLFLGKE
                     LTYGFGIMKRAEMRRQAHDLLCKLGLDIDPKTRAGDLSVGKQQLVEIAKAIASDAKYI
                     VMDEPTAALTDREIQTLFETVRELKNKGISFVYISHRMEEIFAICDRITILRDGQYAG
                     VRDIPKTTFDEIVAMMVGRELGERFPERQHCIGDIKLSVDNLCANHVFENVSFELRKG
                     EVLVLAGLMSAGRTEVAQTLFGARKANSGSIAIDGQAVKIHNPVQAMQHKIGFVTEDR
                     KTEGLLLDFSIQDNIMLTNFKKTCRMGITQPASENQFVQKFIDQLAIRTTDAHLPVKS
                     LSGGNQQKVVLAKWLGTEPDILILDEPTRGVDIGAKKEIYTIINQLAEAGVAILMISS
                     ELPEVIGMADRVLVMHEGRITGEVNKNNMTQENIMHYATGGEKHVLH"
     gene            complement(1775..2164)
                     /gene="rbsD"
                     /locus_tag="PK856_07655"
     CDS             complement(1775..2164)
                     /gene="rbsD"
                     /locus_tag="PK856_07655"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019259650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HRL02280.1"
                     /translation="MKKHGILNRELAGIFAKLGHTDQIVIADCGLPIPDGITCIDLAY
                     ELGKPSFKEVLAAVLADVAVESSTCAQEITVHNAELWQHLESLALNTAQISHEAFKQQ
                     CAEAKVIIRTGEAMPYANIILRSGVIF"
     gene            complement(2442..>3503)
                     /locus_tag="PK856_07660"
     CDS             complement(2442..>3503)
                     /locus_tag="PK856_07660"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HRL02281.1"
                     /translation="GFLSVYEEDTDDDEDGEEGSKKLPDMAEGDVLPVDALRGEQHFT
                     QPPPRYTEASLVKALEEFGIGRPSTYASIISTLKDREYVTLDQKRFTPTDTGDIVNKF
                     LTEHFGQYVDYNFTAKLEDQLDEIASGKRQWQPVMKTFWKDFDKQVKEKEDIPRAQIT
                     SEEIDEVCPKCGDHKLMIKFGKRGRFIACQGYPDCDYTRNVNETAEEAAKAAEEPTEV
                     EGRFCPKCNGQLIYKKGRFGKFIGCSNYNSKPKCTYIEPLEKKDTGVACPKCGKGHLI
                     ERKSRYGKLFYSCETYPDCDYAVWNPPINEPCPKCQWPLLTIKVTKRRGTEKVCPQKE
                     CGYAEQIEAPEAKKATDEA"
BASE COUNT          801 a          814 c          883 g         1005 t
ORIGIN      
        1 ccatcgctaa aatggggtca gtgcctgagg cgagcaaagt ggcagccact gcgcctgtta
       61 aagccaaggt tgagcccaca gacaaatcaa tgccgcgggt caaaatcaca aacgtcatgc
      121 caaacgcaat caaggcgcta atggaaacct gacgcaacac gttcaaaata ttgctggcgg
      181 tgagaaagct tgggttcatc gcggtaatga cgatgatgag caacaacagc ccaaagaatg
      241 ggcccaagcg cgataacaag gctttggtat tagtggagga catgtttttc tcctccggtg
      301 gcgtaatgca tgatgttttc ttgagtcatg ttgtttttgt taacctctcc ggtgatgcgg
      361 ccttcatgca tcaccaaaac acggtctgcc atgccaatga cttcgggcaa ttccgaagaa
      421 atcatcaaga tggcaacccc tgcttcagca agttggttga tgatggtgta aatttctttt
      481 ttagcgccaa tgtccacgcc gcgggtgggc tcatccaaaa tcaagatgtc gggctcggtg
      541 cccaaccatt tggctaaaac cactttttgt tggttgccgc ccgacaggct ttttacgggc
      601 aaatgggcat cagtggtgcg tatcgccaat tggtctatga atttttgcac aaattggttt
      661 tcgctggcgg gctgggtaat gcccatgcga caggtttttt tgaaattggt cagcatgatg
      721 ttgtcttgaa tagaaaaatc caataacaag ccttcggttt tgcggtcttc ggtgacaaag
      781 ccgattttgt gttgcatcgc ttgcacaggg ttgtgaattt tcaccgcttg cccatcaatg
      841 gcgatgctgc cactgttggc tttgcgtgcg ccaaacaggg tttgcgccac ttcggtgcgg
      901 cctgcgctca ttaaacctgc caagaccaat acttcgcctt tgcgcagctc aaacgacacg
      961 ttttcaaaca catggttagc gcacaaattg tctacactca gcttgatgtc gccaatgcag
     1021 tgttggcgct ctggaaaacg ctcgcccaat tcgcggccca ccatcatggc gacaatttcg
     1081 tcaaaagtgg ttttgggtat gtcgcgcaca cctgcatact ggccatcgcg caaaatggtg
     1141 atgcggtcgc aaatggcgaa gatttcttcc atgcggtggg aaatgtacac aaaggaaatg
     1201 cctttgtttt tcagctcacg cacggtttca aacagggttt ggatttcgcg gtcggtaaga
     1261 gcggcagtgg gctcatccat cacaatgtat ttggcatctg aggcaatggc tttggcgatt
     1321 tccaccaatt gctgtttgcc cacagacaag tcgccagcgc gggttttcgg gtctatgtcc
     1381 agtcccagtt tgcaaagcaa atcatgggct tggcgacgca tttcagcgcg tttcatgatg
     1441 ccgaagccat acgtcaattc tttgcctaag aacaggtttt ccgccacgct caagtcgggc
     1501 aagatgttca attcttggtg tatcacatgg atgccggagc gttcagaatc tttgatggtt
     1561 ttgaaggttt gttcttcgcc atcgacgagg atgtggccgc tgtctttgtg gtggatgcca
     1621 ctgaggattt tcatcaacgt ggatttgcct gcgccgtttt cacccatcaa cgcatgtatc
     1681 tcaccattag ccagttcaaa cgacacatcg tttaagactt ggttgccact gaaagatttg
     1741 ctgatgccac tcatttgtat catgggaatg cctcttaaaa aatcacaccg ctgcgcaaaa
     1801 taatattggc ataaggcatt gcctcacctg tacggataat gactttggcc tctgcgcatt
     1861 gttgcttgaa tgcctcgtgc gaaatttgag cggtattcaa ggctaaagat tccaaatgtt
     1921 gccacaattc agcgttgtga acagtgattt cttgcgcgca agtgctggat tctaccgcca
     1981 catcggccaa tacggccgcc aacacttctt tgaatgaggg tttgcccaat tcatatgcca
     2041 aatctatgca ggtgatgcca tcggggatgg gcaagccaca gtcggcaatc acgatttggt
     2101 cggtgtgacc aagcttcgca aaaatacccg ccaattcacg gtttaaaatg ccatgttttt
     2161 tcatgaaata aagagccctt caatctgatg acattgatta tgcacagcaa ggctgcgatt
     2221 gagtaaaatg gtgtgagaaa gggcgttatg gtaacaaatc tgagtggttc ttgagagtaa
     2281 atttgctgga tttgggttat gtctggcatt aataatttat ttgtcattta atattcgcat
     2341 taaatggcaa atgaaaaatg agagagatag tatgtcgata aacacacata aaagcggcct
     2401 gtaaatgctt tacaggccgc ttgaagaagg ttgctgtgct tttaggcttc gtctgttgct
     2461 tttttggcct caggcgcttc gatttgctca gcgtaaccac actctttttg tggacacact
     2521 ttttcagtac cacggcgctt ggtgactttg atggttaaca gcggccattg gcatttcggg
     2581 cacggctcat taatcggtgg attccatacc gcgtaatcgc aatcgggata ggtttcgcaa
     2641 ctgtagaaca atttgccata acggctcttg cgctcgatta aatggccttt gccgcatttc
     2701 ggacacgcca cgcctgtgtc ttttttctcc aaaggctcga tgtaagtaca cttaggcttg
     2761 ctgttgtaat tgctacagcc gatgaattta ccgaaacggc cttttttgta aatcagctga
     2821 ccattgcatt ttggacagaa gcggccttct acttcggtag gctcttctgc tgccttggcg
     2881 gcttcttcgg cagtttcgtt gacgttgcgg gtgtaatcac aatctgggta accttggcag
     2941 gcaatgaagc ggccgcgttt gccaaatttg atcatcaatt tgtggtcgcc acatttcggg
     3001 cacacttcgt cgatttcttc ggaggtgatt tgcgcgcgtg gaatgtcttc tttttctttg
     3061 acttgtttgt caaagtcttt ccagaaggtt ttcatcaccg gctgccattg gcgcttgccg
     3121 ctggcaatct cgtccaattg gtcttcgagt ttggcggtga agttgtaatc tacgtattgg
     3181 ccgaaatgct cggtgaggaa tttgttgacg atgtcgccag tgtcggttgg cgtgaagcgt
     3241 ttttggtcta gcgtgacgta ttcacggtct ttcaatgtcg aaatgatgct ggcgtaagtc
     3301 gatgggcgac caatgccaaa ttcttccaag gcttttacca atgaggcttc ggtatagcgt
     3361 ggcggtggtt gggtaaagtg ttgttcgccg cgcaatgcgt cgactggcaa cacgtcgcct
     3421 tcggccatgt ctggcaattt tttgctgcct tcttcgccgt cttcatcgtc gtcggtgtct
     3481 tcttcataca cgctcaagaa gcc
//
LOCUS       DAOMVK010000219         3502 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_7825_length_3502_cov_2.535248, whole genome shotgun sequence.
ACCESSION   DAOMVK010000219 DAOMVK010000000
VERSION     DAOMVK010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7825_length_3502_cov_2.535248"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..1031)
                     /locus_tag="PK856_07665"
     CDS             complement(186..1031)
                     /locus_tag="PK856_07665"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRL02282.1"
                     /translation="MTESVSKYRHISFTAHYTGYVWYRMGISHPNFATQEGQMYAQLV
                     DPAECLAEFFWGSSIRTTLMQRHEMLDTRLEALIAEHPQVQILEIACGLSPRGWLFRE
                     KYPDIQFVELDLPDMAQMKREALASLQPDAKVIGADLFSDRFAAVFRTFDTSKPLVII
                     SEGLINYFALDLLRTLLQRLIKYTQDFQALYYLTDVYPMPLQAHFRYMAWQASWILKT
                     LSHSDFDFHFNTPGEVATFFHSCGFAQVNLWQPSMFKKQEPAASDIETQSHQGDLVWV
                     IEAQR"
     gene            1476..2693
                     /locus_tag="PK856_07670"
     CDS             1476..2693
                     /locus_tag="PK856_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HRL02283.1"
                     /translation="MKKRLHIGLGLSSALLASMLLINPVYAETYKASNAEKALKIINL
                     NDLEGQVQASMDKGAFGYIRGGAEDEQNLRHNTSSFDKKYIMPRALQGIEFSDLDLKT
                     QLLGIPLSTPIIQAPMAAQGLAHEQGEIATAKGMAKSGSIFSLSTYGNKTIEEVAAAL
                     DKPYFFQLYMSKNEKFNEFTLKRAKENGAKAIILTVDSPVGGYREDDIRNDFQFPLGF
                     ANLELFAKQNDDGSKTGKGAGISEIYVQAKQAFTPADIAYVKKLSGLPVIVKGIQDPQ
                     DADTAIKVGAAAIWVSNHGGRQLDSGPSSFDVLPAIAKVVNKRVPIVFDSGVRRGSHV
                     FKALASGADIVAIGRPILYGLHLGGSEGVHSVVEQLNKELKINMMLGGAKNIEQIKKT
                     KLYTDKSFEQQSQ"
     gene            complement(2874..3116)
                     /locus_tag="PK856_07675"
     CDS             complement(2874..3116)
                     /locus_tag="PK856_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02284.1"
                     /translation="MATVKKRKRALAPNKPTFESGGLAVLAALVGVLFCIVGIGLMYV
                     SHRNGMIYIRLLQFGIISVVLGVIGFIVALKVKPKA"
     gene            complement(3094..>3502)
                     /locus_tag="PK856_07680"
     CDS             complement(3094..>3502)
                     /locus_tag="PK856_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02285.1"
                     /translation="DYHQFSPKRYFWVRKASKPAMLRSTVGTQAVDEHWLDTLCAPVW
                     RLIESRSASGVRAWPELEREQIEAAANQAEALGLALFKKVWQQLAEDASAKHILQAAH
                     VMQLARQSQQQWPVFAIESMETGESTDGNSEEA"
BASE COUNT          994 a          847 c          770 g          891 t
ORIGIN      
        1 tgctgcccga gccaaaattc aacagcgtgt cgtctacatc aaaaaaaatg gccttgtgca
       61 ttgttcgtgt ccttgggtgt catgagctgg cttaagcggc ttattttaac ccatgtcagc
      121 cgtctacgca gtgtgcaaaa gcttacaggc cgcatacaag cggcctgtaa tagattacaa
      181 acacattaac gctgcgcttc gatgacccac accaaatcac cttggtggga ttgtgtttct
      241 atgtcagaag cagctggttc ctgtttttta aacatactgg gttgccatag attcacttga
      301 gcaaacccac atgaatggaa aaacgttgcc acctcgccag gggtattgaa atggaaatcg
      361 aaatcactgt gagacaaagt ttttaaaatc cagctggctt gccaagccat gtaacggaaa
      421 tgggcttgca aaggcatggg gtacacatcg gtcaggtaat acaatgcctg aaaatcttgc
      481 gtgtatttga ttaagcgttg caataaggtg cgcagcaagt ccaaggcaaa ataattgatt
      541 aagccttcgc tgatgatgac aagaggtttg ctggtatcga aagtgcggaa taccgcggca
      601 aaccgatcag aaaacaaatc agccccgatg actttggcgt ctggttgcaa acttgccaac
      661 gcttcgcgct tcatttgcgc catatcaggc aaatccagtt ctacaaattg tatgtctggg
      721 tatttttcgc ggaacaacca acccctaggt gacaaaccac aggcgatttc caagatttgg
      781 acttgtggat gctcggcaat taaggcttct aaacgggtat ccaacatttc atgcctttgc
      841 atgagggtgg tgcgtatgct gctgccccaa aaaaattccg ccaaacactc tgctggatca
      901 accaattggg catacatctg cccttcttgg gtagcaaaat ttggatggga gatgcccatg
      961 cgataccaaa cataacctgt gtaatgcgcg gtaaaagaaa tatggcggta tttggatacg
     1021 gattcggtca tggcaggcct ccagttttca actcgttacc cacaattaat aatagattca
     1081 ataccctatt tatgccagta cccatgcata cttttacgct tgttgggtaa aaagcatgag
     1141 gtttcttaca agatacgtag ccaaatcagt tggcatttat tagagtttac ttactgtaaa
     1201 tgttgttttg atgacattac ctatctttaa gggtaataaa acgagttcca tgttttatcc
     1261 gctactcaag aaagaataaa tctattaaat acccgcatgt attgacagct aattgcccat
     1321 acttggacta aaaatagaac aatatttccc aaatttgtat cttgttgaac attatcatct
     1381 taattaatat ttcaacatca gcggtgcacc aacattcatt gcaactgcac ccatccaaca
     1441 cccgcaattt gaaattgaat gaagaaagaa aaaatatgaa aaaacgactg cacatcggtt
     1501 tgggcttgtc ctcagcatta ttggcttcaa tgctgctcat taaccctgtt tacgccgaaa
     1561 cctataaggc cagcaatgct gaaaaagcct tgaaaattat caacttaaat gatttagaag
     1621 gccaagtcca agccagcatg gacaagggtg cgtttggtta catccgtggc ggtgccgaag
     1681 atgaacaaaa tttgcgccac aacaccagca gttttgataa aaaatacatc atgccgcgtg
     1741 cactgcaagg catagaattc agcgacttgg atttgaaaac ccaacttctg ggcatccctt
     1801 tgtccacgcc catcattcaa gctcccatgg ctgctcaagg actggctcac gagcaaggcg
     1861 agattgccac tgccaaaggc atggcaaaat ctggttccat tttttctttg agcacttatg
     1921 ggaataaaac catagaagaa gtggctgccg ctttggataa gccttatttc ttccagctct
     1981 acatgagtaa gaatgagaaa ttcaacgaat tcaccttgaa gcgggccaaa gaaaacggcg
     2041 ccaaagccat cattttgacc gtagattcac ctgtgggtgg ctaccgcgaa gacgacattc
     2101 gcaacgattt tcaatttcca ttgggctttg ccaatttgga attgttcgcc aagcaaaatg
     2161 acgatggttc aaaaacaggc aaaggcgcag gcatcagcga aatttacgtg caggccaagc
     2221 aagcattcac cccagccgac attgcttatg tgaaaaaact gtcaggtctg cctgtcatcg
     2281 tcaaaggcat acaagaccca caggatgcgg acacagccat taaagtaggc gctgcagcca
     2341 tttgggtttc caaccatggt ggccgccaat tagacagtgg cccgtcatcg tttgatgtgt
     2401 tgcctgccat agccaaagtg gtgaacaagc gcgtgcccat cgtgtttgac agtggcgtgc
     2461 gtcgcggctc acacgtattc aaagccttgg ccagtggtgc tgatatcgtc gccatcggtc
     2521 gcccgatttt gtacggcttg catttgggtg gctctgaagg cgtgcactcg gtggtggaac
     2581 aattgaacaa agaattgaaa atcaacatga tgcttggcgg tgccaaaaac atcgaacaaa
     2641 tcaagaaaac caaattgtat accgacaaaa gctttgaaca acaatcccaa taatccacag
     2701 tggtcagcat aagcctgcaa tgtttgcagg cttttttcac ttcatgggtg cgggcagtga
     2761 gtacaatcta tcaaacacag tgtcgtcact cgattcagca ctcttacaat agcaatgcgg
     2821 cctgtaaaca aatgggtgtt tacaggccgc attatgggta tgattcgcca gattcaggct
     2881 tttggtttga ctttcaaggc cacaataaaa ccgatcacac ccaataccac actgatgatg
     2941 ccaaactgca gaagtcggat gtaaatcatg ccattgcgat gagacacata catcaaccct
     3001 attcccacaa tacaaaacaa cacgcctacc aatgcggcca aaaccgccaa tcctcccgac
     3061 tcaaatgtcg gtttgtttgg tgcaagcgct cgcttacgct tcttcactgt tgccatctgt
     3121 actttcccct gtttccatgc tttcaattgc aaacactggc cattgttgtt ggctttgtct
     3181 tgccaattgc atcacatgcg ccgcttgcaa aatgtgcttg gcactggcgt cttcggccaa
     3241 ttgttgccac acttttttga acaaagccaa gcccaaagcc tcggcttgat tggccgcggc
     3301 ttcgatttgc tcgcgctcaa gctcgggcca agcacgcaca ccactggcgc tgcgcgactc
     3361 aatcaaacgc caaacaggcg cgcacaaggt atccaaccaa tgctcatcga cagcttgcgt
     3421 gcccacggtc gagcgcaaca tcgcaggttt gctggctttg cgcacccaaa aatagcgctt
     3481 gggactgaat tggtggtaat cc
//
LOCUS       DAOMVK010000220         3485 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_7875_length_3485_cov_4.514286, whole genome shotgun sequence.
ACCESSION   DAOMVK010000220 DAOMVK010000000
VERSION     DAOMVK010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7875_length_3485_cov_4.514286"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..1382
                     /locus_tag="PK856_07685"
     CDS             6..1382
                     /locus_tag="PK856_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HRL02286.1"
                     /translation="MLARIQFAFTVSFHIIFPSITIGLASYLAVLEGCYLKTHNPVYK
                     QLYLFWSKIFAVNFGMGVVSGLVMAFQFGTNWSGFSEFAGSITGPLLTYEVITAFFLE
                     AGFLGVMLFGWNKVGPRLHFFATCMVALGTFMSTFWILASNSWMQTPQGYIIENSVVV
                     PTDWLAVIFNPSFPYRLAHMAIAAFITSALLVVATGAWHALKGKRDAAVNKMLSMGLG
                     LLIVLVPLQVLVGDMHGLNTLKYQPAKIAAVEGHFKNEGNEPTPLILFGIPDMEAEET
                     KYKVEIPALGSLILTHSLDKQVPALTEFAKEDRPNSTIVFWSFRIMAGLGMLMLLLAL
                     WGNWLRRGQRWGNKPLFLRFAVLMGPSGLVAVLAGWFTTEVGRQPWVVYGVQRTVDAV
                     SGHGVLPMTISLAVFIVIYCSVFGVGTYYLIHMLKKGPDDALPPETTDMIAASGHVNV
                     ATDTTQQH"
     gene            1393..2397
                     /gene="cydB"
                     /locus_tag="PK856_07690"
     CDS             1393..2397
                     /gene="cydB"
                     /locus_tag="PK856_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HRL02287.1"
                     /translation="MGLDLALIWFVIIVFGTLMYIVMDGFDLGIGILMPFTPKLEDRD
                     TMVNTVAPVWDGNETWLVLGGAALFGAFPLAYAVVLEALMMPLGLMLIALIFRGVAFE
                     FRFKAIESHRGFWDKAFMGGSIVATFCQGVVVGALVQGLEVTGRHYSGGALDWLTPFS
                     LFCGLGLIVAYALLGSTWLMMKTEGELHTMAQRWSRRLVVLLLLAMVVVSVWTPMLDA
                     KIAARWFSLPNFWFFLPVPVLALAASWGIHKAINNNNHISPFLLTLLLIFLGFSGLGI
                     SIWPNIIPPNISIWQAASALQAQGFMILGTLLILPIILGYTAWSYFVFRGKVVDGYDH
                     "
     gene            2408..2515
                     /locus_tag="PK856_07695"
     CDS             2408..2515
                     /locus_tag="PK856_07695"
                     /inference="COORDINATES: protein motif:HMM:NF022085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2474 family protein"
                     /protein_id="HRL02288.1"
                     /translation="MKQYLWLVGIWAASIAALSVFTLLFKLLMRWAGLS"
     gene            2682..3030
                     /gene="rnpB"
                     /locus_tag="PK856_07700"
     ncRNA           2682..3030
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK856_07700"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(3157..>3485)
                     /locus_tag="PK856_07705"
     CDS             complement(3157..>3485)
                     /locus_tag="PK856_07705"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRL02289.1"
                     /translation="ICGVLSYNDFQAHEGSANIGYWLGEAYQGHGIVSSALPVLVQYG
                     FTQLGLKQVFIRVAEGNDKSAAIPKRLGFQLIDTDEGSEILHQQAINHHVYAMRQEDW
                     AGVSAQ"
BASE COUNT          746 a          761 c          977 g         1001 t
ORIGIN      
        1 cttttatgct ggcgcgcatt cagttcgcgt ttacggtctc gtttcacatc attttcccct
       61 ccatcaccat cggtttggca agttatttgg cggtgttgga aggctgttat ttgaaaaccc
      121 acaatcctgt ctacaagcaa ttgtatctgt tttggagcaa aatctttgcc gtcaattttg
      181 gcatgggggt ggtgtctggt ttggtgatgg catttcaatt tggcaccaac tggagcggct
      241 tttccgagtt tgcaggcagc attacgggcc cgttgctcac gtatgaggtg attacagcct
      301 ttttcttaga agcgggtttc ttgggggtga tgctgtttgg ttggaacaaa gtcgggccac
      361 gcctgcattt cttcgccact tgcatggtcg ccttgggcac gtttatgtcg actttttgga
      421 ttttggcttc caattcatgg atgcaaaccc cgcaaggtta catcattgaa aacagtgtgg
      481 tggtgcctac cgattggttg gcggtgatat tcaatccgtc ttttccatac cgcttggcac
      541 atatggcgat tgcggctttc atcaccagtg ccttgttggt ggtggcgacg ggggcttggc
      601 atgcgctcaa aggcaagcgt gatgccgccg tgaacaaaat gctgtccatg ggtttgggtt
      661 tgctcattgt gttggtaccg ctgcaggtgt tggtgggtga catgcatggt ttgaatacct
      721 tgaagtatca gcctgcaaaa attgccgcgg ttgaaggcca ttttaaaaac gaaggcaatg
      781 aacctacgcc gctgattttg tttggcatac ccgatatgga agcagaagaa accaaataca
      841 aagtggaaat tcctgctttg ggcagtttga tactcactca cagtttggac aaacaagtgc
      901 ctgctctgac cgaatttgcc aaagaagacc gaccgaattc taccatcgtg ttttggtcgt
      961 tccgcatcat ggcgggcttg ggcatgttga tgttgttgtt ggcgctgtgg ggcaattggt
     1021 tgcgccgtgg gcagcgttgg ggcaacaagc ctttgttttt gcgctttgcg gtgttgatgg
     1081 ggccatcggg attggttgca gtgttggcag gttggtttac cactgaagtg ggccgtcagc
     1141 catgggtggt gtatggcgta cagcgcacgg tggatgccgt gtctggacat ggcgtgttgc
     1201 cgatgaccat cagtttggcg gtgttcattg tgatttattg ctcggtgttt ggtgtgggta
     1261 cgtattatct gatacacatg ctgaaaaaag ggcctgatga tgctttgcca cccgaaacca
     1321 ccgacatgat agccgcttca ggccatgtca atgtggctac cgacaccacc caacaacatt
     1381 aaggagacga acatgggact ggacttggcc ttgatttggt ttgtcatcat cgtgtttggc
     1441 accttgatgt acattgtgat ggatggtttt gacttgggca taggcatttt gatgccgttt
     1501 acgcccaaac tagaagaccg cgacaccatg gtaaacaccg tggcgcctgt gtgggatggc
     1561 aatgaaacct ggttggtatt gggcggcgcg gctttgtttg gtgcgtttcc cttggcttat
     1621 gcggtggtgt tggaagcctt gatgatgccc ttgggcttga tgctgattgc cttgattttc
     1681 cgaggtgtgg catttgagtt tcgtttcaaa gcgatagaaa gccaccgtgg tttttgggac
     1741 aaggcgttta tgggtggctc tatcgtggcg actttttgcc aaggtgtggt cgtaggtgcg
     1801 ttggtacaag gcttagaggt tacaggccgc cattatagcg gcggtgcttt ggattggttg
     1861 acaccgttca gcttgttttg cggtttgggt ttgattgtgg cttatgcctt attgggctcg
     1921 acttggttga tgatgaaaac cgaaggtgaa ttgcacacta tggcgcagcg ttggagccgt
     1981 cgtttggtgg tgttgctgtt gctggccatg gtggtggtga gtgtgtggac gccgatgttg
     2041 gatgccaaaa ttgcggcacg ttggttcagt ttgcccaatt tttggttctt cttgcctgta
     2101 cccgtattgg cattggcagc ctcgtggggc atacataaag ccatcaacaa caataaccat
     2161 atctcacctt ttctgctcac cttattgctg attttcttgg gcttcagcgg cttgggcatt
     2221 tccatctggc ccaacatcat tccgcctaac atcagcattt ggcaggcggc ttctgctttg
     2281 caggcacaag gctttatgat tttgggtact ttgttgattt tgcccatcat cttaggctat
     2341 accgcttggt cgtattttgt gttccgcggc aaagtggtgg atggctatga tcattgaggg
     2401 aaaaaccatg aaacagtatc tttggttggt gggcatttgg gcagcaagca tcgcggcatt
     2461 gagcgtgttt accttattgt ttaaattgtt gatgcgctgg gcaggcttgt cttgatgtgt
     2521 ataaaaccct gagcaagctg tggataagct gtgaattgtg tggataactt gtgtatgaag
     2581 tggataaatc acggttttaa ggacggcact taaagtaatt tgcatcccaa tgcggcctgt
     2641 aaaaagttca ggccgcattc gtgttataat gcccgcgcat tacagtatgc cagacagtcg
     2701 ccgcgtggca acacggggag gaaagtccgg gctccacaga gcagaatgcc agttaacggc
     2761 tgggcgtcgt gaggcgacgg aaagtggaac agaaagcaaa accgccgatg gccttagggc
     2821 acaggtaagg gtgaaaaggt gcggtaagag cgcaccgggc acttggcaac aaggcgtcgc
     2881 aggctaaacc ccattcggag caagaccaaa cagaatctgt taatgcggct cgcattggat
     2941 tcgggtaggt tgcttgacgg catgagtgat catgttgcta gaggaatgac tgtccaacga
     3001 caaaacccgg cttatcggca cactgtaatg catccccctc acaatttcta tctgtttctc
     3061 ttattgattc catcgcagta aatccatgtg caaactgaat gcatccgtgg taaatattgc
     3121 tgcttaagat gggttgaaaa cacacttaca ggcagcttat tgggctgata cgcctgccca
     3181 atcttcttgc cgcatcgcat acacatggtg gttaatggct tgctgatgca ggatttcact
     3241 gccctcatcg gtatcaatca attgaaagcc caaacgcttg ggaatggcgg cgcttttgtc
     3301 attgccctct gccacccgaa tgaaaacctg ttttaagccc aattgagtaa acccatattg
     3361 caccaacaca ggcaaagcgc tgctgacgat gccatgccct tgataggcct cgcccagcca
     3421 ataaccgatg ttggcagaac cctcatgggc ttgaaaatca ttgtacgaca acaccccaca
     3481 aatct
//
LOCUS       DAOMVK010000221         3479 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_7886_length_3479_cov_3.967874, whole genome shotgun sequence.
ACCESSION   DAOMVK010000221 DAOMVK010000000
VERSION     DAOMVK010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7886_length_3479_cov_3.967874"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..116)
                     /locus_tag="PK856_07710"
     CDS             complement(<1..116)
                     /locus_tag="PK856_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRL02290.1"
                     /translation="MDYYPMFARLQQRPVLVVGAGQVAERKVDSLLQSGALVR"
     gene            213..698
                     /locus_tag="PK856_07715"
     CDS             213..698
                     /locus_tag="PK856_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02291.1"
                     /translation="MRIPSTSACVCCSAVLLSVLLSACGKGLSDEQLQKVTVKAASEA
                     STPSTDLIEPVPLTASSPDPIPTGVEPEALAEEPKAAKPVHEFNPADFPDACNDYVDA
                     VSQCIDVMNKGGLEGTASLQMQLDEMQASWLKETDKSNLNNTCSTALMDIDPALRDLG
                     C"
     gene            692..988
                     /locus_tag="PK856_07720"
     CDS             692..988
                     /locus_tag="PK856_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02292.1"
                     /translation="MLKQGACYVGLSLLIAACKPAAPQAKPLPEPCQRYQAYLQRCEQ
                     LIVGADATRKQLFNQYREQALSQWQQESDQTQLSRSCQQGLDKLQQIFKPQGCE"
     gene            985..1359
                     /locus_tag="PK856_07725"
     CDS             985..1359
                     /locus_tag="PK856_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02293.1"
                     /translation="MMIFRAWMLSATLLGLAACQPNADKTASAPSTATSAPTTTASAA
                     VAPLSASQLAALPDACQQLLSNIEKCVVKYEKLQPEDAQTYRMEYEQLKAQIVQGTDV
                     KGLEAACIESNKQAEQVPETKC"
     gene            1485..2210
                     /locus_tag="PK856_07730"
     CDS             1485..2210
                     /locus_tag="PK856_07730"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HRL02294.1"
                     /translation="MSFFQGWTIPVVDEATHQHLAAKTTELEQRLRDIATRFGAARFA
                     SSLAVEDMVITDVIARLGLNIEVFTLETGRLNPETSALIPATEERYGIKLRVMTPNED
                     AVKRYVNEHGRDAFYESVDLRRECCRIRKIEPLNRAISGADAWLTGQRREQSATREEL
                     PLEEFDANHQIAKFNPIFDWHEADVWAYVEAHQVPVNALYQQHYPSIGCEPCTKPVRA
                     DEDIRAGRWWWENKDTKECGLHK"
     gene            2297..3217
                     /gene="cysD"
                     /locus_tag="PK856_07735"
     CDS             2297..3217
                     /gene="cysD"
                     /locus_tag="PK856_07735"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HRL02295.1"
                     /translation="MSDAISNKHLDWLEAESIYIIREVIAEAKNPALLFSGGKDSVVL
                     LALACKAFSYGERKLQLPFKLLHVDTGHNYQEVIDFRDQTVERLGVELVVGHVEDSIK
                     KGTVVLRSELDSRNAAQAVTLLEAIEDHEFDALMGGARRDEEKARAKERIFSFRDEFG
                     QWDPKAQRPELWSLYNTRLFKGENMRVFPISNWTEMDIWQYIEREGLALPPIYFAHQR
                     EVVEKNGLLVPVTRLTPKKDGDVSEVRSVRFRTVGDISCTCPVASTASTPAEIVAETA
                     QTTVSERSATRMDDQVSESAMEERKKAGYF"
     gene            3289..>3479
                     /locus_tag="PK856_07740"
     CDS             3289..>3479
                     /locus_tag="PK856_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HRL02296.1"
                     /translation="MSTTDSVLRFITAGSVDDGKSTLIGRLLYDSQALLADQVAQLNK
                     RSAGGTLDLASLTDGLEAER"
BASE COUNT          918 a          883 c          921 g          757 t
ORIGIN      
        1 cgcacgagcg caccactctg tagtaaactg tcgactttgc gttccgccac ttgtccagca
       61 ccgaccacca aaacaggccg ttgctgtaag cgtgcaaaca ttgggtaata atccataatc
      121 ttccttcatt tcaggccgct attgtatgcc gagccccgtc aggaagtgaa agaagcaatt
      181 tttgtgaact tagtgaaaat ggttataagc tcgtgagaat cccatctact tcagcgtgtg
      241 tgtgttgttc agcggtatta ctgagtgtgt tattgagcgc ctgtggcaag ggtttgtcag
      301 acgagcaatt gcaaaaagtg acggtgaagg cagcttccga agccagcacg cccagtaccg
      361 atttgataga gcccgtgccg ttaacggcat cgtcaccaga ccccattccc acaggtgtgg
      421 agcctgaggc tttggcggaa gagcccaagg ctgcgaaacc tgtgcacgaa ttcaatcctg
      481 ccgacttccc cgacgcctgt aatgattatg ttgatgcggt gtcgcaatgc atcgatgtga
      541 tgaacaaagg tggcttggaa ggcacggcca gcttgcaaat gcaattggat gaaatgcagg
      601 cttcatggct caaagaaacc gataaaagca atttgaacaa tacttgcagc accgccttga
      661 tggacataga ccctgcattg cgagatttgg gttgttgaaa caaggcgcgt gttatgtggg
      721 tttgagtctg ttgatagcgg cctgtaaacc tgctgcacct caagcaaagc ctttacccga
      781 accgtgtcaa cgctatcaag cctatttaca gcgctgtgaa caattgattg tgggggcgga
      841 tgccacgcgc aaacaactgt tcaaccaata ccgcgagcag gccttgagcc aatggcaaca
      901 ggaaagcgac caaacccaat tgtcccgttc ttgtcagcaa ggtctggaca aactgcaaca
      961 aatctttaaa ccgcaaggat gtgaatgatg atttttcgag cttggatgct gagcgcgacg
     1021 ctattgggct tggcagcctg ccaaccgaat gccgacaaaa cggcctcggc cccatcgaca
     1081 gccacaagcg cgcctaccac cactgccagc gcagccgtgg cgcctttgtc tgccagccaa
     1141 ttggccgcct tgccagacgc ttgtcagcaa ttgctcagca acattgaaaa atgcgtggtg
     1201 aaatacgaga aattgcaacc tgaagacgca cagacttacc gcatggaata cgagcaattg
     1261 aaggcgcaaa tcgtgcaagg taccgatgtc aaaggtttgg aagcggcctg tatcgagagc
     1321 aataaacagg ctgaacaagt gcctgaaacc aagtgctgaa agcaagctgc tataaacgcc
     1381 ttgttgctta tagataattt ggctataaac acataaacga atgttctttc gttattcaat
     1441 ttcaaattgc tacactagcc ccactgcaaa tatggggctt tttcatgtct ttttttcaag
     1501 gttggacgat acctgtggtg gacgaagcca cacaccaaca cttagccgcc aaaaccactg
     1561 agctggaaca gcgtttgcgc gacatcgcca cgcgttttgg cgcggcgcgt tttgcctcga
     1621 gcttggcggt ggaagacatg gtgattaccg atgtgattgc gcgcttgggg ctgaacattg
     1681 aagtgttcac cttggaaaca gggcgtttga atcccgagac ctcagcctta atccccgcca
     1741 ccgaagaacg ctacggcatc aagttgcggg tgatgacccc gaatgaagac gcggtcaaac
     1801 ggtatgtgaa cgaacatggc cgcgatgcgt tttacgaaag cgtggatttg cgccgtgaat
     1861 gttgccgcat ccgcaaaatc gagcctttaa accgcgccat cagcggcgca gacgcatggc
     1921 tcacaggcca acgccgcgaa caatcggcca cgcgtgaaga attgccacta gaagaattcg
     1981 atgccaatca ccaaatcgcc aaattcaatc cgattttcga ttggcacgag gccgatgtgt
     2041 gggcttatgt tgaagcgcac caagtgcctg tgaacgcatt gtatcaacaa cactatccca
     2101 gcataggttg cgagccttgt accaagcctg tgcgcgcgga tgaagacata cgcgcagggc
     2161 gctggtggtg ggaaaacaaa gacaccaaag agtgcggctt gcacaaataa accgccataa
     2221 gcggcctgta aagcttacag gccgcttgtc agcacccaat ttaaaacacc gattaaactt
     2281 gaataaagaa gcagacatga gtgacgcaat cagtaacaaa catttggatt ggctggaagc
     2341 cgaatcgatt tacatcatcc gtgaggtgat tgctgaagcc aagaatccag ccttgctgtt
     2401 ttctggcggc aaagactcgg tggtgttgtt ggccttggca tgcaaagcct tcagctatgg
     2461 cgagcgcaag ctgcaattgc catttaaatt gctgcacgtg gacacaggcc acaactacca
     2521 agaggtgata gatttccgtg accaaacagt agaacgcttg ggcgtggagc tggtggtggg
     2581 ccatgtggaa gattcgatta aaaaaggcac ggtggtgctc agaagcgaat tggattcgcg
     2641 caatgccgcc caagccgtga ccttgcttga agcgattgaa gaccatgaat ttgatgcctt
     2701 gatgggcggc gcgcgtcgcg atgaagaaaa agcccgcgcc aaagaacgca tcttctcttt
     2761 ccgcgatgaa tttggccaat gggacccgaa ggctcagcgc ccagaattgt ggagcctcta
     2821 caatacccgt ttgttcaaag gcgaaaacat gcgcgtgttc ccgatttcca attggacgga
     2881 aatggacatt tggcaataca tcgagcgcga aggcttggcc ttgccgccga tttactttgc
     2941 ccatcagcgc gaagtggtgg aaaaaaatgg cttgcttgtg ccggtgacgc gtttaacgcc
     3001 taagaaagac ggcgatgtca gcgaagtgcg ctcggtgcgt ttccgcaccg tgggcgacat
     3061 ttcttgcacc tgcccagtgg cgtctaccgc gtctacgcct gccgaaattg tggcggaaac
     3121 cgcgcaaacc acggtatcag aacgcagcgc cacccgcatg gacgaccaag tgtctgagtc
     3181 ggcgatggaa gagcgcaaaa aagccggtta tttctgatat tcgaacccca tactcttaca
     3241 ggccgcatgc aatcagtata agcggcctgt aaacaaggaa cacaagcaat gagcacgaca
     3301 gattcagtat tgcgcttcat caccgcaggc agtgtagacg atggcaaatc cactttaatt
     3361 ggccgcttgc tgtacgacag ccaagccttg ctcgccgacc aagtggcgca gcttaacaaa
     3421 cgcagcgcag gcggcacttt ggatttggcc agtttgaccg atggcttgga agccgaacg
//
LOCUS       DAOMVK010000222         3471 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_7910_length_3471_cov_3.716042, whole genome shotgun sequence.
ACCESSION   DAOMVK010000222 DAOMVK010000000
VERSION     DAOMVK010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7910_length_3471_cov_3.716042"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            323..709
                     /locus_tag="PK856_07745"
     CDS             323..709
                     /locus_tag="PK856_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02297.1"
                     /translation="MKNIKKNLLWVTLGLGMATIAIASPSIDKEIDAMVKENNKTMPT
                     DMGDGLMAKSMQRAGKIITLSMRFEDASLSAKDFDVTGGKKVAISQTCSSQLMRRYLD
                     HGYTIQYHYEFQDKTRVTVPVKRSDC"
     gene            829..1281
                     /locus_tag="PK856_07750"
     CDS             829..1281
                     /locus_tag="PK856_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HRL02298.1"
                     /translation="MSAAISVFSLGFTKKTAEQFFALLRNNQVKTVLDVRLNNVSQLA
                     GFAKRDDLRFFLREVGNIDYVAMPDWAPTDGLMKQYKNGDIAAERFQDDFWALLAQRN
                     IERDVPLSLLEHGCLLCSEHEPHQCHRRLVLEYLQEHANLNLHITHLL"
     gene            1527..1796
                     /locus_tag="PK856_07755"
     CDS             1527..1796
                     /locus_tag="PK856_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02299.1"
                     /translation="MKKTIITTVLAALSVSTAMAASIANFTNATEQQNPYAQSNAQVQ
                     SQAVELEQVNYGKVNGKVVNPLFKYTGQSTASQVSAETSSVRVSH"
     gene            2152..3180
                     /locus_tag="PK856_07760"
     CDS             2152..3180
                     /locus_tag="PK856_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRL02300.1"
                     /translation="MISLQNDYSEGAHPRILTLLSETNRVQSAGYGEDAYCLQAASYI
                     KQHLQAPQAAVHFVSGGTQSNTVIIKSMLRPHEAVIAAQSGHIAVHETGAIEATGHKV
                     ITMPVGNHGKLTPEAVRNAVTTHTDEHMVKPRMVYISQTTEIGSVYSVAELRALRACC
                     DELGLYLHIDGARLGAALAANSELSLAIMAEVADVFYIGGTKNGALLGEAIVIVNPDL
                     QTDFRYGMKQHGALLAKGRILGVQFVGLFAENVFFECAAHAHEQAMALAQGLRDLGCG
                     FLTEPQSNQIFPILPNTVITALLEQFAFYHWQDINDTDSAVRLVTSWATEPANVKAFL
                     RAATELLP"
     gene            3268..>3471
                     /locus_tag="PK856_07765"
     CDS             3268..>3471
                     /locus_tag="PK856_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02301.1"
                     /translation="MKSILLIAALSVFSAAAMAQTPAHQHSAVSSSGSDSTQEYKTAM
                     DEMHAGMNIAYTDDADVDFARGMI"
BASE COUNT          949 a          808 c          852 g          862 t
ORIGIN      
        1 gcgttttttt cgaggtcggt gagcatggcc acgcacagaa ggtgaggaaa cagcgttcag
       61 tataaagctt tccgaaacag aagcctatgt ttgtgaccaa caagtgcttg atttgtgtaa
      121 ccgcatccac aagcggcctg taaaagcttg aatgagtatc catgcaacat cgacgagctg
      181 tatttgactc gcagacaggc aagctgcatg cacttgaagc ttcagatgaa aagatggtca
      241 aacattaaaa gagatattgg ggtgattaat tcacgaagtt aaactatgat ttctaaacga
      301 ctcatttacg ctatggatga tcatgaaaaa cattaaaaaa aatttgttgt gggtgacttt
      361 gggtttgggc atggctacca tcgccattgc gtcaccatcc atagacaaag aaattgatgc
      421 gatggtgaag gaaaacaaca aaaccatgcc caccgacatg ggggatggac tgatggccaa
      481 atccatgcaa agagcaggca aaatcattac gctcagcatg aggtttgaag atgccagtct
      541 ctccgcaaaa gattttgatg tgactggtgg aaagaaagtg gcaatatcac aaacttgctc
      601 cagtcagctg atgagacgct atttggatca tggctacacc attcaatatc actatgagtt
      661 tcaagataaa acccgcgtca ccgtgcctgt aaaacgcagc gattgctgac aaattgggtt
      721 tacaggccgc ttgcgcctaa atgaaagact caagctacaa gcaggtgaag cttgtggctg
      781 atatgggtga catattcaat gtggcttttt tcttacgaag gaatgtcgat gtctgctgcc
      841 atttccgtgt tcagcttggg tttcaccaag aaaaccgccg agcaattttt tgccttgttg
      901 cgcaacaacc aagtcaaaac cgtcttggac gtgcgtttga acaatgtgtc gcaattggcg
      961 ggctttgcca agcgcgatga tttgcgcttt tttctgcgtg aagtcggcaa tattgattat
     1021 gtggccatgc ccgattgggc gcccaccgat ggcttgatga agcaatacaa gaatggcgac
     1081 atcgctgccg agaggtttca agacgatttt tgggccttgt tggcgcagcg caatattgaa
     1141 cgcgatgtac cgctttcttt attggagcat ggctgcttgc tgtgcagcga gcatgagcca
     1201 caccagtgtc accgccgttt ggtgttggaa tacctgcaag aacatgccaa tttgaatttg
     1261 cacattaccc atttgctatg aaagtagtga aaccatacga aagcggcctg taaaccgaac
     1321 aatcacggct tacaggccgc ttaaatcttt gagcccacaa taaaaacagc tgtttactgt
     1381 gcctgaaatc accactgggc ttggctttgc tagattatta actttaagtt aacgttgttt
     1441 tgaatatatg cctatttttt aaaccaccga gaatcgataa gatgcagtca tcgaaacaac
     1501 aaaacaacaa ctaaagaggc aagcaaatga aaaaaaccat catcaccacc gtattggccg
     1561 cattgagtgt ttctactgcc atggcagcga gcattgcgaa tttcaccaac gccaccgaac
     1621 aacaaaaccc atacgctcaa agcaatgccc aagtgcaaag ccaagcggtg gagttggaac
     1681 aagtgaatta tggcaaagtg aacggcaaag tcgtgaaccc attgttcaaa tacacgggtc
     1741 aaagcactgc cagccaagtg tctgctgaaa cctcatcagt gcgcgttagc cactaagtac
     1801 ctctcaatcg gctgggttta tcctactcct gaagccaagc tgattcacac aagcggcctg
     1861 taagcatttg caggccgctt gtgcgtgttg tgtttggaca tgagctgaat gagcggcgct
     1921 gcaagaataa cagtttttag aaacttctac agccagcttg ttcagcggct taccatagta
     1981 ctgcggaaac atgaatagca tgccattctg tgtggattca agcagaagtc gacttgagtt
     2041 tgtggcgcgc aaaaccgaag caaaattgac ttgagcgtgt aagtattcat tggcaatggg
     2101 atgggtggcg gtacaatggc tgctttaata atgtaacagg acgcatgtct gatgatttcc
     2161 ttgcaaaacg attacagcga aggtgcccat cctcgcattc tgaccttgct ctctgaaacc
     2221 aatcgggtgc aaagcgcagg ctatggtgaa gatgcgtatt gtttacaggc cgcctcctac
     2281 atcaaacaac acctgcaagc accgcaagcg gcggtacact ttgttagcgg cggcacccaa
     2341 agcaataccg tcatcatcaa gtctatgttg cgcccacatg aggccgtaat cgccgcgcaa
     2401 agtggccaca ttgccgtgca tgaaacgggc gcaattgaag cgacaggtca caaagtcatc
     2461 accatgcctg tgggcaacca tggcaagctc acacctgagg cagtacgcaa tgcagtgacg
     2521 acccacaccg atgagcacat ggtgaagccg cgcatggtct acatttccca aaccaccgaa
     2581 atcggcagtg tgtacagcgt ggctgagttg cgcgcattgc gggcatgttg cgatgagttg
     2641 ggtttgtatt tgcacatcga tggcgcgcgt ttgggcgcgg ctttggcggc aaacagcgaa
     2701 ttgagcttgg ccatcatggc cgaagtggcc gatgtgtttt acatcggtgg caccaaaaat
     2761 ggtgctttat tgggcgaagc gattgtgatt gtgaaccctg atttacagac cgactttcgc
     2821 tatggcatga agcaacacgg tgctttattg gccaaaggcc gcatcttggg tgtgcaattt
     2881 gtgggtttgt ttgctgaaaa tgtgtttttt gaatgcgctg cacacgcaca cgagcaagcg
     2941 atggcactgg cgcaaggctt gcgcgatttg ggctgcggtt ttttgaccga gccacaatcc
     3001 aaccaaatct tcccgatttt gcccaatacc gtcatcaccg ctttgttgga acaatttgcc
     3061 ttctaccatt ggcaagacat caacgacacc gacagcgccg tgcgtttggt cacttcgtgg
     3121 gccactgagc ctgccaatgt taaagcgttt ttacgcgctg ccactgaatt attaccttaa
     3181 tcccttaaat gccaacctga tcgaatgcta tattcaataa gcattttatt gggttgtttt
     3241 ttttcattca caaagtcagg aatccgcatg aaatccattt tattgattgc cgcattaagc
     3301 gtgttttcgg ctgccgcaat ggcgcaaaca cccgcccacc aacactcagc ggtaagcagc
     3361 agcggcagcg acagcaccca agaatacaaa accgccatgg atgagatgca tgctggaatg
     3421 aatattgcat atacagatga tgcggatgtg gactttgccc gcggcatgat c
//
LOCUS       DAOMVK010000223         3465 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_7931_length_3465_cov_2.978006, whole genome shotgun sequence.
ACCESSION   DAOMVK010000223 DAOMVK010000000
VERSION     DAOMVK010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7931_length_3465_cov_2.978006"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PK856_07770"
     CDS             <1..467
                     /locus_tag="PK856_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HRL02302.1"
                     /translation="KGQFMAPEDMGNVIDKARAAAAEKGLPADFMVCERGASFGYHNL
                     ISDMRSLMIMRDTDCPVVFDATHSVQMPGGLGNASGGRREFVPVLARAAIAAGVAGVF
                     METHPNPCEAKSDGPNAVPMEYMKALLTSMKQLDAVVKSQALLENDLLQYGK"
     gene            560..1045
                     /locus_tag="PK856_07775"
     CDS             560..1045
                     /locus_tag="PK856_07775"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRL02303.1"
                     /translation="MNLESLQAERLPTEVPLPMDLLLLADETQEAICAYVHDSEVWVC
                     RQRQDIVAVACVLVLDDSRVELKNLAVAEGMQGKGIGGIFLQQLKQHYRATHTQMLVG
                     TADVGWSQQRFYQSHGFVRVGTRERFFTENYPEPIIENGQQLVDMHVFACDLQDIAQQ
                     P"
     gene            1170..2156
                     /locus_tag="PK856_07780"
     CDS             1170..2156
                     /locus_tag="PK856_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02304.1"
                     /translation="MQGEDWLYRLTGLASDDFASVQQGIVLEAGNHLYSLANHRRLQA
                     GYLSLPALADMSKPSGTGRVRLQELVADVQDLHAHSDNAGAVFQVASQFNLLEMVGPH
                     VRPEDGIAGYVYDQTQGPACAMACAAGTLYRNYFVKLSEQTGQSEAMQLNTLADLEAA
                     LGNAEQGLWEMVNGYLLPYANGLQQVTGLIRGREAELRALIRVGMQADTEVTLPNCGH
                     TVTQVYASAVPMAYASAGERGWEALARLVLEAAYELTLLIAAQSPKKRVFLTRLGGGA
                     FGNPDAWIDDAIVRAIEQVQGADLEVFLVSHLHSHAHNQMLINRCRELGLVE"
     gene            complement(2228..2842)
                     /locus_tag="PK856_07785"
     CDS             complement(2228..2842)
                     /locus_tag="PK856_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02305.1"
                     /translation="MHTLLSLIPRPKRLLPLIGLSSLALLMACTEKPAEATSASAASS
                     TEAPATEASSASAPVIVTPSQRQLSSAQLDMAHAVLEAMKTKQYDSFASHIHPSFAAE
                     LKANPKLMDEMAGFIPHGEPVKPPKLYAMEDVQYEGYGNMLMATFDYPYENRNMLFVI
                     AFDAAEGSTSIKSLAVNSFPGVGDYMEQDEGENTEASSDAQASE"
     gene            complement(2965..>3465)
                     /locus_tag="PK856_07790"
     CDS             complement(2965..>3465)
                     /locus_tag="PK856_07790"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL02306.1"
                     /translation="YGVMVFLMTATPLAMHHHDYAFPDIAVVIQWHVLGMFVPSFFTG
                     HLIKKHGLKPIIMLGCALLIISAIINLFGQSYVHFFTSLVLLGIGWNFTFIGATQLVS
                     LTYRPQEKGKVQGMNDFLVFTTAAIASLFAGQTVETIGWIWVNLISLPLIAVAMLLIA
                     KTRTKV"
BASE COUNT          839 a          773 c         1003 g          850 t
ORIGIN      
        1 aaaaaggcca attcatggcg cctgaagaca tgggcaatgt catcgacaaa gcccgcgccg
       61 ccgctgccga aaaaggcttg cccgctgact ttatggtgtg tgaacgtggc gcaagcttcg
      121 gctaccacaa cttgatttcc gacatgcgca gcctgatgat catgcgtgac accgattgcc
      181 ctgtggtatt cgatgccacc cattcggtgc aaatgccggg cggtttgggc aatgcctcag
      241 gtggtcgccg tgaatttgtg cctgtgcttg cgcgtgcggc catcgccgca ggtgtggcgg
      301 gcgtgttcat ggaaacccac ccgaatccgt gcgaagccaa atcagatggc ccgaacgccg
      361 tgcctatgga atacatgaaa gccttgctca cttcgatgaa gcaactcgat gccgtggtga
      421 aaagccaagc cttattggaa aacgacttgc tgcaatacgg taagtaatcc aacatgctca
      481 aagcggcctg taaacttgaa tgtaaggttt acaggccgct ttgttgtgac acgtatctat
      541 ttatcaaaca gagaaacaca tgaatttgga gagcttacag gcagagcgat tgccaaccga
      601 agtgccattg ccaatggatt tattgctgtt ggccgatgaa acccaagagg ccatttgtgc
      661 ctatgtgcat gacagcgagg tgtgggtgtg tcggcagcgg caagacattg tggcggtggc
      721 ctgtgtcttg gtgcttgatg acagccgtgt ggaattgaaa aatctggctg tggccgaggg
      781 aatgcaaggg aaaggcatcg ggggtatttt tttacagcaa ttgaaacaac actaccgcgc
      841 cacccatacg caaatgttgg tgggcactgc cgatgtgggt tggtcacaac aacggtttta
      901 ccaaagccat ggttttgtgc gtgtgggcac acgtgagcgt tttttcaccg agaactaccc
      961 agagcccatt attgagaatg gccaacaatt ggtggacatg catgtgtttg cttgtgattt
     1021 gcaagacata gcgcagcaac cataagcggc ctgtaaggac tcgctaagca tcaatagaga
     1081 gatattcaca atcatagcga tacggaacgg tgacctatta cgtggtaggc atccattcga
     1141 tgggcttgga aaaataaaaa ggaggggaaa tgcaaggtga ggattggctt taccgcttaa
     1201 cgggtttggc cagtgatgat tttgccagtg tgcagcaagg catcgtgttg gaagcaggca
     1261 atcacttgta cagtttggcc aatcatcggc gcttacaggc cggttatttg agtttgcctg
     1321 ctttggcaga catgtctaag ccctcaggca cggggcgtgt tcgcttgcaa gagctggtgg
     1381 ccgatgtgca agacttgcat gcgcattccg acaatgcagg tgcggtgttt caagtggcat
     1441 cccagttcaa tttgttggaa atggttggcc ctcatgtgcg gcctgaagat ggtattgcgg
     1501 gttatgttta tgaccaaacg caaggccctg cttgtgccat ggcttgcgcg gcgggcacgc
     1561 tctaccgcaa ttattttgtc aaactgagtg agcaaacagg ccaaagtgag gccatgcaat
     1621 tgaatactct ggctgatttg gaggccgcat tgggcaatgc tgagcagggt ttgtgggaga
     1681 tggtaaatgg ctatttgttg ccatatgcca acggtttaca gcaagtgact gggttgatac
     1741 ggggacgaga agctgaactt cgagccttga tacgcgtggg gatgcaggcc gatacggaag
     1801 tgactttgcc taattgtggg cacacggtta cgcaagtgta tgcttcggct gtgcccatgg
     1861 catatgcctc ggcaggagag cgagggtggg aagccttggc acgcttggtg ttggaggctg
     1921 cttatgagct gactttgctc atcgccgccc aatcacccaa aaaacgtgtg ttcctaaccc
     1981 gtttgggagg aggtgcattt ggtaatcccg atgcatggat agacgatgca attgtccgag
     2041 ccatagaaca ggtacaagga gcagacttgg aagtgtttct ggtcagccat ctgcacagtc
     2101 atgcccacaa ccaaatgttg ataaatcgct gccgtgagtt gggcttggtc gagtaagtcc
     2161 atataagcgg cctgtaaggt tttacaggcc gcttatagac atggacaaca ggtgctttta
     2221 aagctgatta ttcacttgcc tgagcatcgc tgctggcttc ggtgttttca ccttcatctt
     2281 gttccatata atctcctacg cctgggaaag aattgactgc caagcttttg atggaagtgc
     2341 tgccttcggc ggcatcaaag gcaatcacaa acaacatatt gcggttttca tacgggtagt
     2401 caaacgtggc catcaacatg ttgccatagc cttcgtattg cacgtcttcc attgcataca
     2461 gtttcggcgg cttcacaggc tcgccatggg ggatgaagcc tgccatttca tccatcaatt
     2521 tggggtttgc tttcagctcg gcggcaaagc ttgggtggat gtgcgaggca aatgaatcat
     2581 attgcttggt tttcatcgcc tcaagtaccg catgggccat gtccaattgg gctgagctga
     2641 gttggcgttg cgaaggggta acgatgacag gtgcagatgc tgaagaggct tctgtggcgg
     2701 gtgcttcagt actgctggct gcgcttgccg aagtggcttc agcaggcttt tctgtgcagg
     2761 ccattaacag cgccaagctg ctgagcccaa tgaggggcaa aaggcgtttc ggtcgcggaa
     2821 tcaaagaaag caaagtgtgc atggggaagc tcttgtgagg gaatgttgta tggtaatcag
     2881 gagtggtatg aaactcaagc ttctgtttta aaggtgaagc agggcttata agcggcctgt
     2941 aaaaaggttt acaggccgct tgtttcagac tttggtacgg gttttggcga taagcaacat
     3001 ggccacagca atcagcggca agctgatgag gttgacccat atccagccta tggtttccac
     3061 ggtttgccct gcaaacaagc tggcgatggc ggcggtggtg aacaccagaa agtcgttcat
     3121 gccttgtact ttgccttttt cttgcggtcg gtaagtgagg ctcacgagtt gggtggcacc
     3181 aataaaggtg aaattccagc ctatgccaag caataccaag gaggtgaaaa agtgcacata
     3241 gctttgcccg aacaaattga tgatggcgct gatgatgagc aaggcgcagc cgagcatgat
     3301 gatgggtttt aagccgtgtt tcttaatcaa gtggccagta aaaaatgagg gcacaaacat
     3361 gcccaacaca tgccattgaa tcaccacggc aatgtcgggg aaggcgtaat cgtggtggtg
     3421 catggccaaa ggcgtggccg tcatcaaaaa caccatcacg ccata
//
LOCUS       DAOMVK010000224         3439 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_7995_length_3439_cov_3.064716, whole genome shotgun sequence.
ACCESSION   DAOMVK010000224 DAOMVK010000000
VERSION     DAOMVK010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7995_length_3439_cov_3.064716"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1379)
                     /locus_tag="PK856_07795"
     CDS             complement(<1..1379)
                     /locus_tag="PK856_07795"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRL02307.1"
                     /translation="MRNTSRTWPKALALLVMVLTSLYLLIGGAWLMSLGGSWHYLIVG
                     ILLAMVSVLYAKNRSSSLWLYSGILLGTLLWGLWEVGSDFWALAPRVDVWFLLGLWLI
                     LPFTGRQLGDAGKPKAAMATIMLAILATLTYSWFNDPQEINGVISRAQPAPAQTAGIA
                     NEDWPAYARTQGGARYSPLTQINEQNVKNLEVAWTYHTGDFKTASDSGETTNQVTPVK
                     VGDTVYICTAHQFLDAIDAKTGKRLWRFDPKLSNTKTFQHLTCRGVSYHDNAQAAGLT
                     TANTAATTPSQICPRKVILPVNDGRLIAVNADTGVACSDFGSNGEVNLQANMPNAYRG
                     GYNPTSPPVVTASSIIIGGSVTDNHSNQEPSGVIRAYDVNTGVLRWVFDTGAEDPNQM
                     PGEAGKFVHNSPNAWAPLAYDEASDTVYVPTGVGTPDIWGGDRTELKERFANSIVALN
                     GSTGKLQWH"
     gene            complement(1628..2698)
                     /locus_tag="PK856_07800"
     CDS             complement(1628..2698)
                     /locus_tag="PK856_07800"
                     /inference="COORDINATES: protein motif:HMM:NF024996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRL02308.1"
                     /translation="MLLLSLPVLAEVTLYGQLKGGVAYSKQPDKEGSIVQMEDYLSKL
                     GLKYTEPIADGTDLIWQVETAISLNGSRDGDMKGWNSYDTFIGIQNDTMGTLRVGYLS
                     DGMFGVQNQKLFVGRGESKGDAADPKGLKIFMRADKRWQGVRYDMPHLNGFDFNITHQ
                     LADNATTEEANAATVVNQATIVAGAYQQQAWYMKGAAGLYKHQSVDTGEVRDAKLYRV
                     LAGYDDKKTYVGLSYQYTDGFQGLWEKRFASVNKPLRPTVKTHEAVISASHKIGRFTP
                     RASYAHGWDETLVGSGRKVGNTSYDQIIIGSDYAFNKSTIAMTSLGWIRKPEDYGHSS
                     NGDNVGIKYSSFSLGLGLRKTF"
     gene            complement(3042..>3439)
                     /locus_tag="PK856_07805"
     CDS             complement(3042..>3439)
                     /locus_tag="PK856_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL02309.1"
                     /translation="RWVSRYRSKYILFWMYAIRVVFVLWFINMPREPWVFYVFAIGIG
                     FTFLATVPPTATLVGKLFGVRYLATLFGLTLFSHQVGGFLGAYLGGLSIVEFGDYRWM
                     WYADMALAALAAVIHLPIREAKITPNHSV"
BASE COUNT          905 a          960 c          734 g          840 t
ORIGIN      
        1 aaatgccatt gcaatttgcc cgtgctgcca ttcaaagcca cgatggagtt ggcaaagcgc
       61 tctttcaact cggtacggtc accgccccaa atatcgggcg tgcccacgcc tgttggcaca
      121 tacacggtat cgctggcttc gtcatatgcc aacggtgccc aagcattggg ggaattgtgt
      181 acaaatttgc ctgcttcacc tggcatttga tttgggtctt ctgcacccgt gtcaaacacc
      241 caacgcaaca cacctgtatt cacatcgtaa gcccgtatca cgcctgaagg ctcttggttg
      301 gaatggttgt cagtcaccga accgcctatg atgatgctgc tggcagtgac cacaggcgga
      361 gaagtcgggt tatagccacc tcggtaggca ttaggcatat tggcctgtaa attcacttca
      421 ccattgctgc caaagtcgct acaagctaca cctgtatcgg cattcaccgc aatcaagcgg
      481 ccatcattca caggcaaaat caccttgcgt ggacagattt gtgatggcgt ggtggcagcg
      541 gtattggctg ttgtcaaacc tgcggcttga gcattgtcat ggtaagacac accacgacac
      601 gtcaaatgtt gaaaggtttt ggtattggac aatttggggt cgaaacgcca caagcgtttg
      661 cctgttttgg catcaatggc atctaaaaat tggtgtgcgg tacagatgta cacggtatcg
      721 cccactttaa ccggcgtgac ttgattggtg gtttcccctg agtcactggc ggttttgaaa
      781 tcgccagtgt ggtaagtcca agccacttcc aaattcttca cattttgctc attgatttgc
      841 gtgagcggcg agtagcgcgc cccgccttgg gtgcgtgcat atgcaggcca atcttcattg
      901 gcaatgcctg cagtctgtgc aggggcaggt tgggccctgc tgatgacacc attgatttct
      961 tgtgggtcgt taaaccagct gtaagtcaat gtggccaaga tggccaacat gattgtggcc
     1021 atggcggctt tgggtttgcc cgcatcaccc aattggcggc ctgtaaacgg caaaatcaac
     1081 cacaaaccca ataagaacca cacatccact cgtggcgcca aggcccaaaa atcgctgcca
     1141 acctcccaca atccccacaa cagcgtgccc agtaaaatgc cgctgtaaag ccacaaactg
     1201 ctgctgcgat tcttggcgta caacacgctg accatggcca acaatatccc cacaatgagg
     1261 taatgccatg agccacccaa gctcatcagc caagcacctc caatcaacaa atacaaactg
     1321 gtcagcacca ttaccagcaa ggccaaggcc ttaggccagg ttcgcgacgt atttctcatg
     1381 acaacaccac tccttaaatc atccatttga tgtgattaca tgcatgctca atggcattga
     1441 gtaaacattt catgccagtt cccacatcgc accacacatc ttgagtcatt acgactgttc
     1501 cacatggcat aagtttttgg ctgaatactc aaaaatcaaa acctcttatg cttacaaaag
     1561 cttaccaatg aataaatttt tgtaggtttg gcgaacatta caggccgcac tcaccttact
     1621 gccgcactta aaatgttttg cgcaaaccca agcccaagct gaaggagctg tatttaatgc
     1681 cgacattgtc accattgctg ctgtgaccat agtcttctgg tttgcgtatc cagcccaatg
     1741 aagtcatggc tatggtggat ttgttgaaag cataatcact gccgatgatg atttggtcgt
     1801 aagaggtatt gccgactttg cggccactcc ccaccaaggt ttcatcccag ccatgtgcgt
     1861 aagaggctct aggagtgaag cgcccgattt tgtgcgaggc agaaatcact gcttcatggg
     1921 ttttcaccgt cgggcgtaac ggcttattca cactggcaaa gcgcttttcc cacaagcctt
     1981 gaaagccatc cgtgtattgg taagacagtc ctacataggt ttttttgtca tcataccccg
     2041 ccaatacccg atacaacttg gcatcgcgca cctcaccagt gtccacgctt tggtgtttgt
     2101 acaggcctgc tgcccctttc atataccaag cttgctgctg gtaagcacct gccactatgg
     2161 tcgcttggtt cacgacagtt gccgcattgg cctcttccgt ggtcgcatta tcggccaatt
     2221 ggtgggtgat gttgaaatca aatccattca aatgcggcat gtcataccgc acgccttgcc
     2281 aacgcttgtc tgcgcgcatg aagattttca aacctttggg gtctgccgcg tcgcctttgg
     2341 actcaccacg ccccacaaac agtttttgat tttgcactcc gaacataccg tcgctgaggt
     2401 agcccactcg caaagtgccc atagtgtcgt tttgtatacc tataaaagtg tcgtaactgt
     2461 tccaaccttt catatcgcca tcacggctgc cattcaaaga aatcgccgtt tccacttgcc
     2521 aaatcaaatc cgtgccatcc gctattggct ctgtgtattt caggcccaat ttcgacaaat
     2581 agtcttccat ctgcacaatc gagccttctt tatcaggctg tttgctgtat gccacgcccc
     2641 cttttaactg gccgtataaa gtcacctcag ccaagacagg caaactcaac aacagcacac
     2701 agactccata ccccaagcgt aacaccatcc aaaccccttt tcttcctaca aaatggccat
     2761 caccaaaccg attgaacggt tctgaccctg ttcatgcaaa ttggtttaat ccccatcatc
     2821 actcacagaa aaatctattt gtgttccacc ttccacccaa accaaaatcc atcaacctag
     2881 gtacccttat ctcatcggat tgatacaaca ctttcggttg aaacaaaacc acaagcataa
     2941 gattgatagc gtggtagcaa tgcatgaaaa caaatagtaa caactgcctt aaatggttta
     3001 caggccgcat caagcggcct gtaaactttc aaacaaggga tttaaacact gtgattgggt
     3061 gtgattttcg cttcacgtat gggcaaatga atcaccgccg ccaatgcggc caaagccatg
     3121 tcggcatacc acatccagcg gtaatcccca aattcaacaa tcgacaagcc gcccaaatac
     3181 gcccccaaga aaccaccgac ttgatgcgaa aacagggtca agccaaacaa ggtggccaaa
     3241 tagcgcacgc caaacaattt gccaaccaaa gtcgctgtcg gtggcacagt cgccaagaaa
     3301 gtaaagccta tgccgatggc aaacacataa aacacccaag gctcgcgcgg catattgatg
     3361 aaccacaaca caaacaccac ccgtattgca tacatccaaa acaagatgta tttgctgcgg
     3421 tagcgcgata cccaacgcc
//
LOCUS       DAOMVK010000225         3432 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_8021_length_3432_cov_3.558188, whole genome shotgun sequence.
ACCESSION   DAOMVK010000225 DAOMVK010000000
VERSION     DAOMVK010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8021_length_3432_cov_3.558188"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..451)
                     /locus_tag="PK856_07810"
     CDS             complement(209..451)
                     /locus_tag="PK856_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02310.1"
                     /translation="MQYLWVYLRRSVRLYPRGVDLYLFVEWGLLLVLTLIWLWGDGLS
                     GYVLLASCTGILLSYGHGFLLLRWMRHFARRNGIAE"
     gene            complement(524..1126)
                     /locus_tag="PK856_07815"
     CDS             complement(524..1126)
                     /locus_tag="PK856_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HRL02311.1"
                     /translation="METTHITTIAATSMGEHKAKGSRFLAYAYPLSRADDLKPILQTL
                     HEEHHKAVHWCYAYRLGHEGTLFRANDDGEPSGSAGMPILRQIDSAGLTDLVVVVVRY
                     FGGTLLGVPGLIQAYKQATVLALQAASRLEKPILKTVNIQFAYEKMSEVMRLIKRCDA
                     QIVTQQMDMQVSMEVAVPLAFANAFWQQLEDWHWLIIQKE"
     gene            1227..1712
                     /locus_tag="PK856_07820"
     CDS             1227..1712
                     /locus_tag="PK856_07820"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HRL02312.1"
                     /translation="MDWWTHFLNMLHSEFSDIKDLTELFRMMIRLLLAAILGGLLGLE
                     RESSGKNAGIRTHMLVAMGSAFFMMVPWLLGVDAGNTSRIMQGVITGIGFLGAGSIMK
                     ADKENEVKGLTTAAGIWLTSAIGIACGLGLQTLAILGTLLAFAILWAMPRLSKLGKKQ
                     Q"
     gene            1798..2277
                     /gene="trmL"
                     /locus_tag="PK856_07825"
     CDS             1798..2277
                     /gene="trmL"
                     /locus_tag="PK856_07825"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HRL02313.1"
                     /translation="MPLHIALYQPEIPANTGNIARTCAGTHTSLHLIRPLGFSTDDKM
                     LKRAGLDYWAHVDIHYHDDLEAFIAHTEAQNGKVFLIETYSDVPFSEHDFSNQAQDIY
                     FLFGRETTGLPQEFAQARAQDCLRIPQSEHIRSLNLSNSAAIVIYEALRQQGYPNLA"
     gene            complement(2507..>3432)
                     /locus_tag="PK856_07830"
     CDS             complement(2507..>3432)
                     /locus_tag="PK856_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRL02314.1"
                     /translation="VLIIAEHNNNTLNPATLHAVAAAQKLGAVHVLVAGNAAAAVADQ
                     AKQVAGVEKVLLSESAQFENALPEEVAPLVVSLAGAYSHIAATATAFGKNLLPRIAAL
                     LDASQVSDVTEIIDAQTFTHPIYAGNAFETIKTAETKLVLTIRATAFDAAAATGGNAA
                     VETVAAIPAQNLSRFVGRELTKSDRPELTQAKIIVSGGRAMGSAEKFNELLTPLADKL
                     GAAIGASRAAVDAGYAPNDYQVGQTGKVVAPSLYVAVGISGAIQHLAGMQDSKVIVAI
                     NKDPDAPIFAVADYGLVGDAFDLVPELVNAL"
BASE COUNT          910 a          905 c          798 g          819 t
ORIGIN      
        1 tggaccaaca gcaaagccaa tggcgcaaca aacgcagcca aagctgccaa caagcggcca
       61 gtggtggcgg cagcgatgcc gagcgccaaa acattttggc cagctgtgag gccaaggcca
      121 atcgcgagcg tttgaatgaa ttgaagcaat accatgtgcc caattgatga ctgtgcatga
      181 gcaaaaacca gcctaaggct ggtttttttt attcggctat tccattgcgc cgtgcaaaat
      241 gccgcatcca tctcagcagc aaaaaaccat ggccatatga caacaaaatt cctgtgcatg
      301 atgccaacaa cacataccca gacagtccat ctccccacag ccaaatgagc gtcagcacca
      361 gcaacaatcc ccattcaaca aacaggtata aatccacgcc tcggggatac agccgtacgc
      421 ttcggcgcaa atacacccac aaatactgca taatggcaaa attccagcca attttgagcc
      481 caagtaagcc agctagcacc caaaaaagca gcatcaacat ggcttattct ttctgaatga
      541 tcaaccagtg ccaatcttca agttgctgcc aaaatgcgtt ggcaaacgcc aaaggcacgg
      601 ccacctccat gctcacttgc atgtccattt gttgcgtcac aatctgcgca tcacaacgct
      661 tgattaagcg catcacctcg ctcatttttt catacgcaaa ttgaatgttc acggttttta
      721 aaatgggttt ttccaaacga gatgcggcct gtaaagccaa aaccgtggcc tgtttgtaag
      781 cctgtatcaa gccaggcacg ccaagcagtg tgccaccgaa atagcgcacc accaccacca
      841 cgaggtctgt gaggcccgca gaatcgattt ggcgcaagat gggcatccct gcactgcctg
      901 atggctctcc atcgtcattg gcgcgaaata gagtgccttc gtggcccaat cgataggcat
      961 aacaccaatg cacggctttg tgatgctctt cgtgcagggt ttgcaagatg ggtttcaaat
     1021 cgtcggcgcg gctcaatggg taggcatagg ccaagaaacg gctgcctttg gccttgtgtt
     1081 cgcccatgct ggtggcggca atggtggtga tgtgtgtggt ttccatgtgc ggcattataa
     1141 acaagctttt acaggccgcc catccaagga ttgaatcatt tgccttgttc gcattctaaa
     1201 aaccatgact gcataaggag cacgaaatgg actggtggac ccactttctc aacatgttgc
     1261 acagtgagtt ttccgacatc aaagacttga ccgaattgtt tcggatgatg atacggctgt
     1321 tgttggctgc gatattaggc ggcttactcg ggctcgaacg tgaatccagt ggcaaaaatg
     1381 caggcatacg cacccacatg ctggtggcca tgggttcggc atttttcatg atggtaccgt
     1441 ggttattggg cgtggacgcg ggcaatacca gccgtatcat gcaaggggtg attacaggca
     1501 ttggcttttt gggcgcaggc agcatcatga aggcagacaa ggaaaatgag gtcaaaggtt
     1561 tgaccacagc cgcgggcatt tggctgactt cagccatagg cattgcctgt ggattgggct
     1621 tacaaacctt ggcgattttg ggcactttgt tggcttttgc gattttatgg gccatgccac
     1681 gcttaagcaa actgggcaaa aaacagcaat agcctgttgt ttgaactaca atattgcctt
     1741 atcacaccgt tcataacagc ttgagaacgg tggttttcat gccgaaagat ttgttttatg
     1801 cctttacaca tagccttata ccagcccgaa attcccgcca atacgggcaa catcgcccgc
     1861 acttgcgcag gcactcacac cagcttgcac ttgatacgac cattggggtt ttccaccgat
     1921 gacaaaatgc tcaagcgcgc aggcttggat tattgggcac atgtggacat ccactaccac
     1981 gatgatttag aagccttcat tgcccatacc gaagcacaaa acggcaaggt ttttttgatt
     2041 gaaacctaca gcgatgtgcc tttcagcgaa catgatttca gcaaccaagc acaagacatt
     2101 tactttttgt ttggccgcga aaccacgggt ttaccacaag agtttgccca agcgcgggcg
     2161 caagattgtt tacgtattcc tcaatccgaa cacatccgct cgctgaattt gtccaatagc
     2221 gccgccatcg tgatttacga agccttgcgc caacaaggct atccaaactt ggcttgagtg
     2281 tttgaacata aatttaagcg gcctgtaaat gtttacaggc cgcttttttg cagcatctta
     2341 cggtggctgt gtttgttatt catttaaagc tgccccaccc cttttaacat agccatcaaa
     2401 cgcccgcccg taggctgcat tgatttttcc gatgtgctac tgttaccgaa gcccaacaaa
     2461 aaaacgctcc gcgtagcgga gcgttcgaaa attatttcag caaggattac agagcattta
     2521 ccaactctgg caccaaatca aatgcatcgc ccaccaaacc ataatccgca accgcaaaaa
     2581 tcggcgcatc agggtctttg ttgatggcaa caatcacttt actgtcttgc ataccggcca
     2641 aatgctgaat cgcacccgaa ataccaaccg ccacatacag actaggtgcc accactttgc
     2701 cggtttgacc cacttggtag tcgttaggcg cataacccgc gtcaaccgca gcgcgtgaag
     2761 caccaatagc cgcgcccaat ttgtctgcca atggcgtgag caattcattg aatttctcag
     2821 cactgcccat ggcacggcca ccagacacaa taattttggc ttgtgtcaat tcaggacggt
     2881 cagatttggt caattcacgg cccacaaaac ggcttaagtt ttgagcaggt atggccgcaa
     2941 cggtttcaac cgctgcatta ccacccgtcg cagccgccgc atcaaatgcg gtcgcacgaa
     3001 tggtcaatac caatttggtt tcagctgttt tgatggtttc aaacgcattg cccgcataaa
     3061 tcgggtgggt aaaggtttgc gcatcaatga tttcagtcac atcagacact tgtgaggcat
     3121 ccaacaaagc cgcaatgcgt ggcaacaggt ttttaccaaa cgcagtggca gtggcagcaa
     3181 tgtggctgta agcacctgcc aaagacacca ccaaaggcgc aacttcttca ggcaaggcat
     3241 tttcaaattg agcggattca gaaagcaata ctttttcaac acccgccact tgtttggctt
     3301 gatcggcaac ggctgcggca gcattgccag caaccaaaac atgcacagcg cccaattttt
     3361 gcgcggcagc aaccgcgtgc aaagtggctg gattcaaggt gttattgttg tgttcagcga
     3421 taatcaaaac tg
//
LOCUS       DAOMVK010000226         3430 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_8028_length_3430_cov_3.272296, whole genome shotgun sequence.
ACCESSION   DAOMVK010000226 DAOMVK010000000
VERSION     DAOMVK010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8028_length_3430_cov_3.272296"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PK856_07835"
     CDS             complement(<1..253)
                     /locus_tag="PK856_07835"
                     /inference="COORDINATES: protein motif:HMM:NF017987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChuX/HutX family heme-like substrate-binding
                     protein"
                     /protein_id="HRL02315.1"
                     /translation="MTTLWQRYQDLKAEQAYLFQYDAARQLGVSEGELLASAPDSIYL
                     GSDFVGIFNRLQAIQLVQLVVRNQCAVHEKITHITPLKLN"
     gene            complement(347..1141)
                     /locus_tag="PK856_07840"
     CDS             complement(347..1141)
                     /locus_tag="PK856_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRL02316.1"
                     /translation="MKLNVPLNDEHQLFASYARGFKAPQYDARGAASHSVLNGMIQYY
                     NVPNFDLKPEESDNFELGWQHEGNTTHAKITGFYNRYKNFIEESQTIRTLYPTGRPMP
                     MVMELSSVNLDKVVTYGLEARGQYDFAPYWNVNGSLFWMRGINKADDSHMNSELPMKL
                     RLGLAYANETWGANADWTLSRSKTEYATADKSTPKTPGYGLVDVGGFWNINKNAKLTL
                     NAYNVFDKKYWLPADMLWLNTTGQLDKQDSYSQMGRAISAGIQVKF"
     gene            complement(1138..1962)
                     /locus_tag="PK856_07845"
     CDS             complement(1138..1962)
                     /locus_tag="PK856_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRL02317.1"
                     /translation="MLKSSYNSGNQGWAATVGGAARNDVASGLIMYTHRDYAEIQTAG
                     KQASEQDNSSNNVLLKGKLDLGEHHLTATGEYFKRDGHSKDLLSINSFDDTNSERKRA
                     SLEYQYLPDTAGVLQAVKAGYDYQDLASDMLQTEPSRNLLNRHLQGYQQTQHRAYTQG
                     LWQFDAGIEHNVLAGIDYRHVKTTTNRHSANFDLATDAQVGAVDDLLYYPANTKAVWS
                     AFIQDRMVFDNGVSLTPALRYSHEKSTPNSADFERLKNQANVDFQQMIAGGSYSPA"
     gene            complement(1969..2529)
                     /locus_tag="PK856_07850"
     CDS             complement(1969..2529)
                     /locus_tag="PK856_07850"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRL02318.1"
                     /translation="MPRSHRPIVRCVYCALAVLSASATMAAETANSPSADTELPAIHV
                     EAQKGDNNSANSQTISRKDLDKAGAEDLKTMLRYEPGVEVEAQAGLNNGNFNIRGISE
                     DRVWVMVDGLNLPDSFKDSFWYGGGGARGKNGMTFGQNLVEPDTINSVSVVKGPFEAQ
                     YSNEAIGGAVNMRTYDPADLINDGQQ"
     gene            2725..3054
                     /locus_tag="PK856_07855"
     CDS             2725..3054
                     /locus_tag="PK856_07855"
                     /inference="COORDINATES: protein motif:HMM:NF009840.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme oxygenase"
                     /protein_id="HRL02319.1"
                     /translation="MIVVTNRIRVKTGHGAQMAPRFTRSKSLCEFKGFHKVEVLVAEL
                     DDCDEMSVNMYWDSRADFQVWRESDAFKAAHKRPEGSSSTESPVISSQLLIAEVAAVL
                     ENEAESH"
     gene            3201..3276
                     /locus_tag="PK856_07860"
     tRNA            3201..3276
                     /locus_tag="PK856_07860"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3234..3236,aa:Phe,seq:gaa)
     gene            3351..3426
                     /locus_tag="PK856_07865"
     tRNA            3351..3426
                     /locus_tag="PK856_07865"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3384..3386,aa:Phe,seq:gaa)
BASE COUNT          745 a          778 c          958 g          949 t
ORIGIN      
        1 cattcagttt taatggggtg atgtgggtga ttttttcatg cacagcgcat tggttacgca
       61 ccaccaattg cactagttgt atcgcttgca agcggttgaa aatgccaaca aagtcgctgc
      121 ccaaataaat cgaatcggga gcgctggcga gcaattcgcc ttcgctcacg cccagttggc
      181 gcgcggcgtc gtattggaat aaataggctt gctcggcttt caagtcttgg tagcgttgcc
      241 ataaggtggt catgttgtgt ctcctgctgt gtggggctta caggccgctt gtgagatgca
      301 atgcggcctg taaaccctgt atctgtttaa aaatggtgtg atgcaatcaa aatttcactt
      361 gaatgccagc gctgatggcg cggcccattt ggctgtagct gtcttgtttg tccagttggc
      421 cagtggtgtt cagccacaac atgtcggctg gtagccagta tttcttgtcg aacacgttgt
      481 aagcattgag cgtgagcttg gcatttttgt tgatgttcca aaaaccaccc acgtctacca
      541 agccgtagcc tggggttttg ggcgtggatt tgtcggcagt ggcgtattcg gttttggagc
      601 ggctcagtgt ccaatcggcg tttgcgcccc aagtttcatt ggcataggcc aggcctaggc
      661 gcagcttcat tggcaattcg ctgttcatgt ggctgtcgtc ggctttgttg atgccgcgca
      721 tccaaaacag gctgccattc acattccaat acggggcaaa atcatactgg ccgcgcgctt
      781 cgaggccgta agtcaccact ttgtccaaat tcaccgagct caattccatc accatcggca
      841 tagggcggcc tgtagggtag agtgtgcgta tggtttggct ttcctcaatg aagtttttgt
      901 agcggttgta aaagcctgtg attttggcgt gggtggtgtt gccctcgtgt tgccagccga
      961 gttcaaaatt gtcggattct tcaggtttca aatcgaaatt gggcacgttg taatactgaa
     1021 tcatgccgtt taacacgctg tgagaagccg caccacgggc gtcgtattgc ggcgctttaa
     1081 agccacgtgc atatgaggcg aacaattggt gttcatcgtt cagcggcaca ttcagtttca
     1141 agctgggctg tagctgccgc cagctatcat ttgttggaaa tccacattgg cttggttttt
     1201 caagcgctca aaatcggcgc tgttgggcgt ggatttctcg tggctgtagc gcagggcagg
     1261 ggtgagcgac acgccattgt caaacaccat gcggtcttga ataaaggcgc tccacactgc
     1321 tttggtgttg gcggggtaat acagcaaatc atcgactgcg ccgacttgtg catcggtggc
     1381 aaggtcgaaa ttggcgctgt gtcggttggt ggtggttttg acgtggcggt agtctatgcc
     1441 tgccaagaca ttgtgctcga tgcccgcatc aaattgccat aaaccttggg tataagcgcg
     1501 gtgttgggtt tgctgatagc cttgtaaatg gcggttgaga aggttgcgac tgggttcggt
     1561 ttgcaacatg tccgaagcca aatcttggta gtcataacct gctttaacgg cctgtaacac
     1621 tccagcggta tctggcaagt attggtattc caagctcgcg cgtttgcgtt cgctgttggt
     1681 gtcgtcaaag ctgttgatgc tgagcaagtc tttggaatgg ccatcacgct tgaagtattc
     1741 acctgtggcg gtgaggtggt gttcgcctaa gtccaatttg cctttgagca aaacattgtt
     1801 gctgctgttg tcttgttcgc tcgcttgctt gcctgcggtt tggatttcgg catagtcgcg
     1861 gtgggtgtac atgattaagc cagaggccac atcattgcgc gccgcgccac caacggtagc
     1921 ggcccagcct tggttgccgc tgttatagct gcttttgagc aaggcgcctt attgttgacc
     1981 atcgttgatt aaatcggcag ggtcgtaagt gcgcatattg accgcgccgc ctatcgcttc
     2041 gttggagtat tgggcttcaa acgggccttt gaccacgctg acgctgttaa tggtgtcggg
     2101 ctcaaccagg ttttggccga atgtcatgcc atttttaccg cgcgcgccgc cgccaccata
     2161 ccagaagctg tctttaaagg aatcgggtag gttcaaacca tccaccatca cccacacgcg
     2221 gtcttcggaa atgccgcgga tattgaaatt gccattgtta agcccagctt gcgcctctac
     2281 ttccacgcca ggttcgtagc gcagcatggt ttttaaatct tccgcgcccg ctttgtccaa
     2341 atctttgcgg ctgatggttt gactgttggc gctgttgttg tcgccttttt gggcttcgac
     2401 atgtatggcg ggtaattctg tgtcggcgga tgggctgttt gccgtttctg cggccatggt
     2461 ggctgaggca gataaaacag ccaaggcgca gtaaacacag cgaacaatcg ggcgatgcga
     2521 gcggggcata attgaccttc aatgatgaaa tgatgagaat atttttatta atgattatga
     2581 tatcgattat catttgtatt attaaggtgg cttaaagttt tgtcaagcaa ggtaatcctg
     2641 actttacaca gatgcagagg ctctcattcc cagttaagct agcgggcagt gagacaagca
     2701 cacaatgaaa ataggagcag taagatgatc gtggtgacga atcgaattcg agtaaaaaca
     2761 ggccatggcg cacaaatggc accgcggttt acccgctcta aaagcctgtg tgaattcaaa
     2821 ggttttcata aggttgaggt tttggtggct gaattggatg attgtgatga aatgagtgtg
     2881 aacatgtatt gggacagccg tgctgacttt caggtttggc gtgagtcaga cgcgtttaaa
     2941 gcggcacaca agcggcctga aggcagctcc agcactgaaa gccctgtcat cagcagccaa
     3001 ttgctcattg cagaggtggc tgcggttttg gaaaatgaag ctgaatccca ttaagcggcc
     3061 tgtaaattgg ggagttactt aagaggtgtc agttaaatcg caagtaaaaa caaagcgttt
     3121 tcatgccgag catgtgatgg tgaggggaaa gtgcttgcat cacaaggata ttaatgtttt
     3181 aatacgcatc tcttaagcgc ggccagatag ctcagtcggt agagcaacgg attgaaaatc
     3241 cgtgtgtcgg cggttcgatc ccgcctctgg ccaccactta agccgccgct tatcagcggt
     3301 attttttatt tcagtagttg cagtattcaa acgtgtcatg tacacatcat ggccagatag
     3361 ctcagtcggt agagcaacgg attgaaaatc cgtgtgtcgg cggttcgatc ccgcctctgg
     3421 ccaccatttg
//
LOCUS       DAOMVK010000227         3424 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_8051_length_3424_cov_3.820718, whole genome shotgun sequence.
ACCESSION   DAOMVK010000227 DAOMVK010000000
VERSION     DAOMVK010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8051_length_3424_cov_3.820718"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="PK856_07870"
     CDS             <1..695
                     /locus_tag="PK856_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HRL02320.1"
                     /translation="ASFGLYGKPTTINNTETFASVPFIIRDGAASFNQFGLENNFGTK
                     LFSVSGHVERPGNYEVPLGTPFSELLEMCGGMKDGKKLKAVIPGGSSAPVIPADIMMQ
                     TNMDYDSIAKAGSMLGSGAVIVMDEDVCMVKALERLAYFYYEESCGQCTPCREGTGWL
                     YRIIHRIENGEGRPEDLDLLESVGDQMAGKTICALADAAVMPVQGMMKHFRDEFEYHI
                     EHKKCKCNHKWC"
     gene            725..3013
                     /gene="nuoG"
                     /locus_tag="PK856_07875"
     CDS             725..3013
                     /gene="nuoG"
                     /locus_tag="PK856_07875"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HRL02321.1"
                     /translation="MLQIEIDGKELSVEQGSTVIEAALKSGTYIPHFCYHKKLSIAAN
                     CRMCLVEVEKQRKPLPACATPVTDGMKVFTQSKVAKDAQKGVMEFLLINHPLDCPICD
                     QGGECQLQDLSVGYGKSTGRYEEDKRSVVGKDMGPLVSAAEMSRCIHCTRCVRFTDEI
                     AGWQELAMANRGEFSEIEPMRGKTLETELSGNVIDLCPVGALTSKPFRYDARSWELSR
                     RKSISAHDALGSNLIVQVKSHQVRRVLPLENEAINECWLSDRDRFAYEGLNHAERLAQ
                     PMIKHGGEWHETDWQTALTYVAKGLKGVAADHGANAISVFANANSTVEELLLAKQLAA
                     GLGVNSIDTRMRQQDFNLDVAQQGALWLGQSINDFGSSNAVLVVGSNLRQEQPLLTAR
                     IRQAVKEGMSLSVIGSSEQQLRMHANQHIAHPATWVEVLTQLTNPAADASEFIAATAK
                     QLAEGEKTSIVLGADVVNHPQFAQIYAAAQALAAASNSVVGVLPQAANSVGADVIGFK
                     AANNAVNPVSALNGSQKAVMLLNIEPDMDWAGSETAKQALLQAQTVIALTSFQSESLR
                     ELADVMLPVTPSTETSGSFVSMEGRLQSFHGVVKPYAQSRPMWKVLRVMGNELGLDGF
                     DYTSSEEVLARACINSDSIVGKLNNQVQNIAATGLQAASGLVRVGGVGIYQTDSVVRR
                     SAPLQATVHAAIPAASVHPKTLAALQLTDAAQVVVKSGAASVLVAVVADESIAENVVS
                     LPLHPQNKALGAMNNAIELGRG"
     gene            3016..>3424
                     /locus_tag="PK856_07880"
     CDS             3016..>3424
                     /locus_tag="PK856_07880"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009119545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HRL02322.1"
                     /translation="METWFIGLLGPTWGFVVSSLIKIVIILAPLILCVAYMTYFERKV
                     IGFMQLRVGPQVTGPFGLIQPFADVVKLLMKEIIRPTLANKGLYLLAPVLSLMPAFAA
                     WAVIPFSENWFLTNVDASLLYIMAITSFGVYGVI"
BASE COUNT          846 a          726 c          915 g          937 t
ORIGIN      
        1 cagcttcatt tggtttgtac ggcaaaccga ccaccatcaa caacaccgaa acctttgcgt
       61 cggtgccatt catcattcgt gatggtgcgg ccagcttcaa tcaatttggt ttggaaaaca
      121 acttcggtac caaattgttt tcggtttcag gtcacgttga gcgcccaggt aactacgaag
      181 tgccattggg cacgccgttc tctgagttgt tagagatgtg tggcggtatg aaagacggca
      241 aaaaactgaa agcggtgatt cctggtggat cttctgcacc agtgattcct gctgacatca
      301 tgatgcaaac caatatggat tacgattcta ttgccaaagc tggctcgatg ttgggttccg
      361 gtgcggtcat cgtgatggat gaggacgttt gcatggtgaa agccttggaa cgtttggctt
      421 acttctatta tgaagaatcg tgtggtcaat gtacgccttg ccgtgaaggc acgggttggt
      481 tatatcgcat catacaccgc attgaaaatg gtgaaggccg tcctgaagat ttggacttgt
      541 tggagtctgt gggggaccaa atggcgggta aaaccatttg cgccttggct gacgctgcag
      601 tgatgcctgt acaaggcatg atgaaacact tccgtgatga gtttgagtac cacattgagc
      661 acaaaaagtg taaatgcaac cataaatggt gttaacaccc gtgtgaaata ggattaccta
      721 atatatgtta caaattgaaa ttgatggtaa ggaattatcg gtagagcagg gcagcacggt
      781 catcgaagct gctttgaaat ccggtaccta tattccgcat ttttgttacc ataaaaaatt
      841 gtcaattgct gccaactgcc gtatgtgctt ggtagaagtt gaaaaacaac gcaagccttt
      901 gcctgcttgt gcgacccctg tgaccgatgg catgaaagtg ttcactcagt ctaaagtggc
      961 caaagatgca caaaaaggtg tgatggagtt tttgctgatt aaccacccat tggattgtcc
     1021 gatttgtgac caaggtggtg agtgtcaatt gcaagacttg tcggttggct acggtaaatc
     1081 tacaggccgc tatgaagaag acaagcgctc agtggttggc aaagacatgg gtccattggt
     1141 gtcagcagct gaaatgtcac gctgtatcca ctgtacccgt tgcgtgcgct tcaccgatga
     1201 aattgcgggt tggcaagagt tggccatggc caaccgtggt gagttctctg aaatcgagcc
     1261 tatgcgtggc aaaactttgg aaacagagtt gtctggtaac gtgattgact tatgcccagt
     1321 aggcgcattg accagtaaac cgttccgtta tgatgcccgt tcatgggagt tgtctcgtcg
     1381 taaatccatt tctgctcacg atgccttggg ttctaacttg attgttcaag tgaaatctca
     1441 ccaagtgcgc cgcgtgttgc cattggaaaa cgaagccatt aatgagtgct ggttgtcaga
     1501 ccgtgaccgt ttcgcttacg aaggtttgaa ccatgctgag cgcttggcac agcctatgat
     1561 taaacacggc ggtgagtggc acgaaaccga ttggcaaacg gctttgactt atgtggccaa
     1621 aggtttgaaa ggcgttgcag cagatcatgg tgccaatgca atttctgtgt ttgccaatgc
     1681 caattcaact gttgaagaat tgttgcttgc caagcaattg gctgctggtt tgggtgtgaa
     1741 cagtattgat acccgcatgc gtcaacaaga cttcaatttg gatgtggctc agcaaggcgc
     1801 attgtggtta ggacaaagca tcaatgattt tggcagcagc aatgccgtgt tggtggtggg
     1861 cagcaatttg cgccaagaac aacctttgtt gactgctcgc attcgtcaag ccgttaaaga
     1921 aggcatgagc ttgagcgtca tcggtagcag cgaacaacaa ttgcgcatgc atgctaatca
     1981 gcacattgct catccagcaa cttgggtaga ggtgttgacg cagttgacca atccagctgc
     2041 tgatgcaagt gaattcattg ctgccacagc caaacaattg gctgaaggtg aaaaaaccag
     2101 catcgttttg ggtgctgatg tggtgaacca tccacaattt gcgcaaatct acgcagcggc
     2161 tcaagccttg gctgcggcaa gcaactcagt agtgggtgtg ttgccacaag ctgccaatag
     2221 cgtgggtgcg gatgtgattg gtttcaaagc cgcaaacaat gcagtaaacc cagtttcagc
     2281 tttaaatggc agccaaaaag cggtgatgtt gttaaacatc gagccagaca tggattgggc
     2341 gggcagtgaa acagccaaac aggctttgct ccaagcacaa actgtgattg cattgacgtc
     2401 attccaaagt gaaagcttgc gtgagttggc cgatgtgatg ttgccagtga ccccatctac
     2461 cgaaacttca ggcagctttg tcagcatgga aggtcgtttg cagagtttcc atggtgtggt
     2521 gaaaccttac gcacaatctc gtcctatgtg gaaagtcttg cgtgtgatgg gcaatgagct
     2581 gggcttggat ggtttcgatt acacctcttc tgaagaagta ttggcgcgtg cttgcatcaa
     2641 cagtgacagc attgtgggta aattgaacaa tcaagtacaa aacattgctg ccacaggttt
     2701 acaggccgca tctggcttgg tacgtgtggg cggtgtgggt atttaccaaa ctgattcagt
     2761 tgtgcgtcgt tctgcgccgt tacaagccac cgtacatgca gccattcctg ctgccagcgt
     2821 gcatcctaaa actttggccg cattgcaatt gaccgatgcc gctcaagtgg tggtgaaatc
     2881 tggtgcagcc agcgtgcttg ttgctgtggt ggctgatgaa agcatcgctg agaatgtggt
     2941 gtccttaccg cttcatccac agaacaaagc attgggtgcc atgaataacg caattgagtt
     3001 aggtagaggt taatcatgga aacttggttt attggtctgt taggtcccac ttggggattt
     3061 gtggtgtcat ccttaatcaa aatcgtcatt attttggcgc cattgatttt gtgcgtggcc
     3121 tacatgacgt attttgaacg taaagtgatt ggtttcatgc agttgcgtgt gggcccacaa
     3181 gtcacaggtc cgtttggttt gattcaacct ttcgccgacg tggtgaaatt gttgatgaaa
     3241 gaaatcattc gccctacttt ggcgaacaaa ggcttgtatt tattggcccc tgtgttgtca
     3301 ttgatgccag cgtttgctgc ttgggcggtg attccgtttt cagaaaactg gttcttaacc
     3361 aatgtagacg caagcttgct ttacatcatg gccatcacct cttttggtgt gtacggcgtg
     3421 atca
//
LOCUS       DAOMVK010000228         3422 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_8057_length_3422_cov_2.888031, whole genome shotgun sequence.
ACCESSION   DAOMVK010000228 DAOMVK010000000
VERSION     DAOMVK010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8057_length_3422_cov_2.888031"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PK856_07885"
     CDS             complement(<1..317)
                     /locus_tag="PK856_07885"
                     /inference="COORDINATES: protein motif:HMM:NF020858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HRL02323.1"
                     /translation="MSYTQSFIIVAENSDFPLQNLPYGVFQPQGEHARVGVAIGAEVL
                     DLAALEAAGLLPTGAQNVFGQTSLNAFIALGRPVWQQMRARLQQLLSADCAELRDNTD
                     LCA"
     gene            680..1438
                     /locus_tag="PK856_07890"
     CDS             680..1438
                     /locus_tag="PK856_07890"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRL02324.1"
                     /translation="MEKQSNGVQSLEVGLSVLNVLVEAGAALMLKEVAAAADMHPAKA
                     HRYLVSLVKCGYAKQQTDGRYGLGNAALTMGLAATRRSDGLQIAQPCLQQLHQEVGEY
                     LQVARWTPLGPLVVQFLDNEFGIPISAKVGAIMPILRSATGRVFASFLPETMVKPLVE
                     QAAQDHDAEVWQAFQTQKQRIVQQGYCVVEGEMVLGINAIAAPVFDANNDVVFVLTCL
                     AAADKIHVGFDGAQVQAVLQAARTISAQLGQHRS"
     gene            1517..2038
                     /locus_tag="PK856_07895"
     CDS             1517..2038
                     /locus_tag="PK856_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02325.1"
                     /translation="MDKKPLLGMNKDDDGQIILPDDTYQILVLERPSNNEVLHELFAI
                     FELIHQFHSDNWPDDSFWCQHLPFSFVSKFSLFYIEDLDYLYENPNGTMHHESWEFLS
                     WLDALKERGWEWWSGDLNETTLTIVLLINDIPERLDAMLEIFRILHLNVTRHFVNYYS
                     EKEIRHLNLYEYD"
     gene            complement(2107..3195)
                     /locus_tag="PK856_07900"
     CDS             complement(2107..3195)
                     /locus_tag="PK856_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HRL02326.1"
                     /translation="MDITAAVTAGQGAAFSIETVQLDAPKDQEVLVRIVASGVCHTDA
                     VARDLAIAPLPAVLGHEGSGVIEAVGSNVPELQVGDHVVLSFSYCGECEMCLTGHPTS
                     CATFNQLNFGGCNHDHSHRIHQHDKPLSTFFGQSSFGTYAVAHYHNVVKVDKEVDLAL
                     LGPLGCGIQTGAGTVLNRIKPEFGSSIVVYGSGAVGMSAIMAAKIVGCAHIIAVDIHD
                     SRLALAQELGATATINGSREDVVARIKEITGGGSHYAVETTGVPPVVRQSLQALRPLG
                     TCAIVGITPKMEIDVFADLMAEGKTMMGVIEGDAVTKVFIPKLVDYYKRGQFPFDKLV
                     QFFDFDHINEAFEASKDGSVIKPILRIG"
BASE COUNT          858 a          788 c          902 g          874 t
ORIGIN      
        1 ttagcgcaca aatcagtgtt gtcacgcaat tcggcacaat cggccgacag caattgctgc
       61 aagcgcgcac gcatttgttg ccacacgggg cgacccaagg cgatgaacgc attcaagctg
      121 gtttggccaa acacattttg cgcgcctgtt ggcaacaaac ctgcggcttc taaggccgcc
      181 aaatccaaga cttcagcgcc tatggccaca cccacacggg catgttcacc ttgcggttga
      241 aacacgccat aaggcaggtt ttgcagtggg aaatcgctgt tttcggcaac gatgatgaat
      301 gattgcgtgt atgacattga atctcactcc ttaaagcagg cagctgcgct gaatagggtt
      361 gaatcacaga ataacaaggt ggtttgcatg tcttgaatgt gctcacagtt ccactttcaa
      421 gatggcaaac accgatagaa aacagtgttt tgcacgcacc aattgaaagc cattatagtg
      481 gcattttgaa tttacgcaaa aaataatttt attacgaata gcgtaatttc agaccgattt
      541 caggcatact gctgacatga gcatcagttt atagacgatg tacagataat aggtggcaat
      601 tgcctctgca tgacttcgtg gcggcagtca gtgtcttact caagcctgtg atgattctgt
      661 ccgtttaaca aggaagacta tggaaaaaca atccaacggt gtccaatcat tggaagtggg
      721 tttgtcggtt ttgaatgtgt tggtggaggc gggagccgcc ttgatgctca aagaagtggc
      781 tgcggccgct gacatgcacc ccgccaaggc tcatcgctat ttggtcagct tggtcaaatg
      841 cggctatgcc aagcagcaaa cagacggccg ctatggcttg ggcaatgccg ctttgaccat
      901 gggcctggcc gccacgcgcc gcagtgatgg cttacaaatc gcccaacctt gcttgcaaca
      961 attgcatcaa gaagtggggg agtatttaca agtcgctcgt tggacgccgc tagggccgct
     1021 ggtggtgcaa ttcttggaca atgaatttgg catccctatc agcgccaaag tcggcgccat
     1081 catgcccata ttgcgctcgg ccacagggcg ggtgtttgcc agcttcttgc ctgaaaccat
     1141 ggtcaaacca ttggtagagc aggctgccca agaccatgat gccgaagtat ggcaggcgtt
     1201 tcaaacgcaa aaacagcgca tcgtgcagca aggttattgt gtggttgaag gcgagatggt
     1261 gctgggcatc aatgcgattg ccgcgcctgt gtttgatgcc aacaacgatg tggtgtttgt
     1321 gctgacgtgt ttggctgctg ccgataaaat ccatgtggga tttgatggtg cgcaagttca
     1381 ggcagtgtta caggccgctc gcaccatttc tgcacaattg ggccaacatc gcagctgaca
     1441 agcgactgtg tttggcttga atagaaaggg aggaacgaga gttgctatat ggaaggtgat
     1501 taaaaggagg agtaatttgg acaagaaacc gttattaggt atgaacaaag atgatgatgg
     1561 acaaataatt ttgcctgacg acacttatca aatattggta ttggagcgac caagtaataa
     1621 tgaagtattg catgagctat ttgcaatttt tgaattaatt catcagtttc attcagataa
     1681 ttggcctgat gattcatttt ggtgccagca tttaccgttt tcttttgtat ccaagttcag
     1741 tttgttttac atcgaagatt tggattatct gtatgaaaat cctaacggga ctatgcatca
     1801 tgagagttgg gagtttctat cttggttaga tgcgttaaaa gaacgagggt gggaatggtg
     1861 gtcgggtgat ttgaatgaaa ccactttaac catagtattg ttgattaacg atattcctga
     1921 gcgcttggat gccatgctgg aaatttttag gatattgcac ttgaatgtca ctcgtcattt
     1981 tgtgaattat tattctgaga aggaaatcag acatttgaat ttgtatgaat atgactgatg
     2041 ttgtgaaaca agcggcctgt aaacatttac aggccgcttg tttgcgaatc acgtcgagag
     2101 taaagcttaa cctatgcgta atatcggttt aatcaccgaa ccatctttgg atgcttcaaa
     2161 cgcttcattg atgtggtcga aatcaaagaa ttgcaccaat ttatcgaatg ggaattggcc
     2221 gcgcttgtaa taatcgacca gcttcggaat gaagactttg gtcacggcat cgccttcaat
     2281 cacgcccatc atggttttgc cttcggccat caaatcggcg aacacatcga tttccatttt
     2341 cggggtgatg ccgacgatgg cgcatgtgcc cagtgggcgc aaagcctgta aagattgacg
     2401 caccacgggc ggtacccctg tggtttccac ggcgtaatgg ctgccgccgc ccgtgatttc
     2461 tttgatgcgg gccaccacgt cttcacgact gccgttgatg gtagcggtag cgcccaattc
     2521 ttgtgccaaa gccaagcggc tgtcgtggat gtcgacggcg atgatgtggg cacagcccac
     2581 gatttttgcc gccataatcg cactcatgcc caccgcgcct gagccgtaca ccacgatgga
     2641 cgagccaaat tcaggtttga tgcggttgag caccgtgcct gcgcctgttt ggatgccgca
     2701 gccgagcggg ccgagcaagg ccaaatccac ttctttatcg actttgacca cattgtggta
     2761 atgcgccacg gcgtaagtac caaaagagga ttggccaaaa aaagtagaca aaggcttatc
     2821 gtgttggtga atgcggtggc tgtggtcatg gttgcagcca ccaaaattga gttggttaaa
     2881 cgtggcgcaa gaagtggggt ggcctgttag gcacatttca cattcgccgc aatacgaaaa
     2941 cgacaacacc acatggtcgc ccacttgcaa ctcgggcacg ttgctgccca ctgcttcgat
     3001 gacaccagag ccctcgtggc ccaataccgc aggcaagggc gcgatggcca aatcgcgggc
     3061 aacggcatcg gtgtggcaca cgcctgaggc gacaatgcgc accaacacct cttggtcttt
     3121 gggggcatcc aattgcacgg tttcgatgga aaatgcggcg ccttggccag cggtgactgc
     3181 tgcggtaatg tccatgggtt ttctcttctg tgcttgttga tttgtagcct tatgatacaa
     3241 aaatgcccag ttcaatacag ctgttattac acatatgagc tatgctttac ttgaagggaa
     3301 tctcgagcgc gcagatgcaa aaagaccgcc taaaaagacg gtcttttttt aataaggtgg
     3361 cgagccgaac ccccggcact ggcgacttca aagtgggctg ctggcttccg cccctgaccc
     3421 gt
//
LOCUS       DAOMVK010000229         3413 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_8091_length_3413_cov_3.597379, whole genome shotgun sequence.
ACCESSION   DAOMVK010000229 DAOMVK010000000
VERSION     DAOMVK010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8091_length_3413_cov_3.597379"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(324..1091)
                     /locus_tag="PK856_07905"
     CDS             complement(324..1091)
                     /locus_tag="PK856_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL02327.1"
                     /translation="MNDLFHISGLKVAFGQRVVLDIADLHIPNHATTAIIGPNGAGKS
                     TLLKALIGLYPSRELRCLNEDAAQVLKQGKIAWVGQHEVFATPLTLLEYVLLGRYPHL
                     GWLSRPSARDITYAETLLAEFELAAFADKRLQALSGGEKQRAALVRALLQDTQILLLD
                     EPNNHLDIKHQHQLMQHIQRYQSHKNVSSVMVLHDLALAANYCDEVILLGHGQVIAQG
                     KPQDVMTAAHLQQAYQWPITPELRNGVWYFDTFQAAV"
     gene            complement(1088..2062)
                     /locus_tag="PK856_07910"
     CDS             complement(1088..2062)
                     /locus_tag="PK856_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRL02328.1"
                     /translation="MRSLVKSLPYGRVSLPKALLWLLGSALLIWLCLGVGLGEWQRPD
                     QIDPMIWELRWPRVLAALMVGMSLAVAGAAMQALFANPLADPSLIGTSSGAALGVVIV
                     LAFGLGSLGVSLAAFLGAMTVCLLVLSVHHLLGSGRLGLLIIGFAISSFCLAVVSLIL
                     FVSDDMVLRSATNWLAGNLGAAGYTPIHYAATIMVLGLFLLISKSRDLDILLLGDETA
                     RSMGISVQRSRILIVVATAMLTGAAVSLSGVIGFIGMMVPNVIATVWGGTRASIMWRS
                     AGIGALFLLLVDTIARHIVFPIDLPVGIVISLLGAPFFLWLFVKGMRT"
     gene            complement(2046..2852)
                     /locus_tag="PK856_07915"
     CDS             complement(2046..2852)
                     /locus_tag="PK856_07915"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRL02329.1"
                     /translation="MKSPMKTLLLISMLGLGMAQAQAAERIVVLSSDIADIMVALKAE
                     SLVVGRDSLSKAPSLAQAKNIGMFRNLSAEPIVALKPTVVLGSHMAHPESIYGQLNRH
                     KIKAVNVIPIQSEQSFAKGIVEVGKYAGKSSQAGALSQTWLRQMQARSKTGKRYVFTY
                     DGQIVAGKNTVADTLIRLAGGVNAAANIDGMKPIARETWATLKPDVVVIAQHSLPMVG
                     GSVAAFKQRPEMATSAAARNNKVVSMTAGEAFSLDLNSPKVVDKLHALAR"
     gene            complement(2849..>3413)
                     /locus_tag="PK856_07920"
     CDS             complement(2849..>3413)
                     /locus_tag="PK856_07920"
                     /inference="COORDINATES: protein motif:HMM:NF017022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ChuX/HutX family heme-like substrate-binding
                     protein"
                     /protein_id="HRL02330.1"
                     /translation="QHQPTSAPIVALSRDDAADFQERWRNLSNIHHFFGLLDEFKLSR
                     KQAFEAAPRGYAFKVDNSCVEKAYEAAAAAEVSLITFVGNHGIIQIQTGTVKHVSRQR
                     GWLNIFDQKHNSFTMHIDDSKIESTWLLLRPTRDGIVSCLECLDAFGHTVLTVFGQRT
                     EGQAELAAWRDVLQAATGKTVQDEVMA"
BASE COUNT          804 a          909 c          932 g          768 t
ORIGIN      
        1 atggctggcg gtcatggctc gtttggcacc aaaatgggtg attctttggt ggcttatgca
       61 ttgcctgaat ccaaatgatg cagtgatggt gtattgaagc ttacaggccg cttaatgcgg
      121 cctgtaaata ttttactcga tacaattgtg cttgaatcag gaatgtctgc ccacattgtt
      181 tgccatcatc catgtggcag aggatgtgat tgaatgcatt caagttgata agtttgattg
      241 gcaaacaatt acaggccgca ttaagcggcc tgtaagcatt gcgtagcttg attcaaggtt
      301 tagttggcag tttgccttgt tgttcaaacc gctgcttgaa acgtatcgaa ataccacacg
      361 ccgttgcgca gctcgggggt gatgggccat tggtaggctt gttgcaaatg ggcggctgtc
      421 atcacgtctt gtggtttgcc ttgggcgatg acttggccat ggccgagcaa aatcacctca
      481 tcacagtaat tggccgccaa ggccaagtcg tgtaacacca tcaccgagct gacgttttta
      541 tggctttggt agcgctgaat gtgttgcatc aattggtgtt ggtgcttgat gtcgaggtgg
      601 ttgtttggct cgtctaaaag caggatttgc gtgtcttgca acagcgcgcg caccaaggcg
      661 gcacgttgct tttcgccgcc agacaaggct tgcaagcgct tgtctgcaaa cgcggccaat
      721 tcaaattcgg cgagcaaggt ttcggcatag gtgatgtcgc gggcgctggg gcgtgagagc
      781 cagcctaggt gcggatagcg gcccaataac acgtattcca acaaggtcaa tggcgtggca
      841 aacacctcgt gttggcccac ccaagcgatt ttgccttgtt ttaacacctg agcggcatct
      901 tcgtttaaac agcgcaactc acggctgggg tacaggccga ttaaggcttt taacagggtg
      961 gatttgcctg cgccattggg gccgatgatg gcggtggtgg catggttagg gatgtgcaaa
     1021 tcggctatgt ccaaaaccac gcgttggcca aaggccactt tcaggccgct gatgtgaaac
     1081 aaatcgttca tgtgcgcatg cctttcacaa acaaccacaa gaaaaacggt gcgccgagca
     1141 gcgaaatcac gatgcccacg ggcaaatcaa tggggaacac aatgtggcgg gcgatggtgt
     1201 ctaccaacag caaaaacaac gcgcctatgc ctgccgagcg ccacatgatg ctggcgcgtg
     1261 tgccgcccca aacggtggcg atgacattgg gcaccatcat gccgataaaa ccaatcacgc
     1321 cagacaacga cactgccgcg cccgttaaca tggcggtggc gacgacgatg aggatgcgcg
     1381 aacgctgcac agagatgccc atggagcggg cggtttcatc gcccaacagc aaaatgtcca
     1441 aatcacgcga tttgctgatg agcaaaaaca agcccaaaac catgatggtg gctgcgtagt
     1501 ggatgggggt gtaacctgct gcgcccaaat tgcccgcgag ccaattggtg gcgctgcgca
     1561 aaaccatgtc gtcagacaca aacaagatga ggctgaccac ggccaaacaa aacgagctga
     1621 tggcaaagcc gatgatgagc aagcccaagc ggcctgaacc cagtaaatgg tgtaccgaca
     1681 gcaccaataa acacaccgtc atcgcgccca aaaaagcagc caaagacacg cccaaactgc
     1741 ccaagccaaa cgccagcact atcaccacgc ccaaagccgc accactggaa gtgccaatca
     1801 ggctggggtc ggccaatggg ttggcaaaca aggcttgcat cgccgcgccc gccaccgcta
     1861 aagacatgcc caccatcaag gccgccaaaa cacgtggcca acgcaattcc caaatcatcg
     1921 ggtcgatttg gtctggcctt tgccattcgc ccaagcccac gcccagacac aaccaaatca
     1981 acaaagcgct gcccagcagc cataacaagg ctttgggcag ggaaacgcga ccataaggaa
     2041 gcgatttaac gagcgagcgc atgcagtttg tccaccactt tcgggctatt caaatccaag
     2101 ctgaaggctt cgcctgcggt catgctcacc actttattgt tgcgagcggc tgcactggtg
     2161 gccatttcag gccgctgttt gaaggcggca acactgccgc cgaccatggg caaactgtgt
     2221 tgggctatca ccaccacatc gggcttgagc gtggcccaag tttcacgggc aatcggtttc
     2281 atgccatcga tgttggccgc ggcattcacg ccgcctgcca aacgaatcaa ggtatcggcg
     2341 acggtgtttt tgcctgccac gatttgccca tcgtaagtga acacatagcg tttgcctgtt
     2401 ttggagcggg cttgcatttg gcgcaaccaa gtttgcgata aagccccagc ttgggaggat
     2461 ttacccgcgt attttcccac ttccacgatg cctttggcga atgattgttc gctttggatg
     2521 ggaatcacat tcacggcttt gattttgtgg cggtttaatt ggccgtaaat gctctctgga
     2581 tgtgccatgt ggctgcccaa caccacggtt ggctttaagg cgactatggg ttcggcgctc
     2641 aaattgcgaa acatgccgat gtttttggct tgcgccaagc tcggcgcttt gctcaagctg
     2701 tcgcggccga ccaccaagct ttcggctttc aaagccacca tgatgtcggc gatgtcgctg
     2761 ctgagcacca caatgcgttc cgccgcttgg gcttgtgcca tgcccaagcc caacatgctg
     2821 atgagcagca aggttttcat tggcgatttc atgccatcac ctcatcttgc actgttttgc
     2881 ccgtagcggc ctgtaacaca tcgcgccaag ccgctaattc ggcttggcct tcggtgcgtt
     2941 gcccaaacac ggttaacacg gtgtgaccaa acgcatccaa gcattccaag cagctgacga
     3001 tgccatcacg ggtagggcgc agcagcaacc aagtcgattc gattttgctg tcgtcgatgt
     3061 gcatggtgaa gctattgtgt ttttggtcga aaatattcaa ccagccacgt tggcgtgaca
     3121 catgtttcac cgtgccggtt tggatttgaa tgatgccgtg gttgccgaca aaggtaatca
     3181 acgacacttc agcggcagcg gcggcttcat aggctttttc aacacagctg ttgtcaactt
     3241 tgaacgcata gccacgcggc gcggcttcaa aagcctgttt gcgcgacaat ttgaattcgt
     3301 ctaacaagcc aaagaaatgg tggatgttgc tcagattgcg ccaacgctct tggaaatcgg
     3361 ccgcatcatc gcggcttaag gcgacaatcg gcgcggaagt gggctggtgt tgg
//
LOCUS       DAOMVK010000230         3408 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_8110_length_3408_cov_2.883984, whole genome shotgun sequence.
ACCESSION   DAOMVK010000230 DAOMVK010000000
VERSION     DAOMVK010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8110_length_3408_cov_2.883984"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PK856_07925"
     CDS             complement(<1..267)
                     /locus_tag="PK856_07925"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRL02331.1"
                     /translation="MKIIVIGASGILGQAVVTHLHALGHDVITASRNSGMEQVDIRDD
                     ASVRALFVRTGKVDAIVSTAGVVSFEPLRSMTSAQFMLGLQDTFL"
     gene            380..1279
                     /locus_tag="PK856_07930"
     CDS             380..1279
                     /locus_tag="PK856_07930"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRL02332.1"
                     /translation="MDKMRALEVLVCVVEHGSFSKAADTLDMSAVMVGKYIAALESEL
                     GTRLIERSTRLQKLTAAGNAFYQQAKLTLAQLQHTFTVVEQLQAVPTGTLTISAPPSF
                     GAQTIAPISAAFQALHPTVKIVLDLSNRRVDVLQEDIDIAIRIGTLHTDATVAKHLCS
                     YRMAIGAAPAYLQHAGTPHNLDDLYQHRVLVHRLWHDLDPEQYPHLQTLQLMQEQSTF
                     VSNDGLALRIAAEAGAGIIVQPEVLLQDAFASGRLLRVLPHIALPEQPVHVLYAPHSK
                     ALPKVALFVPFLLQHITKYVQMA"
     gene            complement(1436..2011)
                     /locus_tag="PK856_07935"
     CDS             complement(1436..2011)
                     /locus_tag="PK856_07935"
                     /inference="COORDINATES: protein motif:HMM:NF025432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HRL02333.1"
                     /translation="MDESRNLMNAAQTNALLSVLQSRFEHTRVHFADVAWPDIALALQ
                     QHATALWSLAQMEASGGEPSVLAYAADKGLLWFADAAPESPKARRSLCYDEAALMART
                     KHPPQGSAMAQAAAMGVSLINAEQYQVLQRIWPCDLKTSSWLATPDAIRRLGGALFGD
                     RRYDKVFVYHNGADSYYAARGFRCVLSLSLH"
     gene            complement(2145..3407)
                     /gene="rho"
                     /locus_tag="PK856_07940"
     CDS             complement(2145..3407)
                     /gene="rho"
                     /locus_tag="PK856_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRL02334.1"
                     /translation="MHVSELQRQHVSELLTLAEQHGIENANRLRKQDLVFAIVRALVK
                     KGESFTGKGTLEILPDGFGFLRSPDTSYLAGPDDIYVSPSQIRRFNLHTGDTIEGSVR
                     TPKDGERYFALVKLDIINGDDPEKCKHKILFENLTPLFPTRQFKLERDGKNKEDKNVT
                     SRAIDLVAPIGFGQRALLVAPPKTGKTVMLQNIATAITTNYPEVELIVLLIDERPEEV
                     TEMTRSVRGEVVSSTFDEPAQRHVQVAEMVIEKAKRLVEHGKDVVILLDSITRLARAY
                     NTVVPASGKILTGGVDANALHRPKRFFGAARNLEEGGSLTIIATALVDTGSRMDDVIY
                     EEFKGTGNMELHLERRLAEKRVFPAISVNRSGTRREELLVPNDQLQRMWLLRKFLHPM
                     DDVEAMEFLQDKIKSSKNNADFFDQMKR"
BASE COUNT          805 a          918 c          841 g          844 t
ORIGIN      
        1 taagaatgta tcttgcaaac ccagcatgaa ttgcgcgctt gtcatgctgc gcaaaggctc
       61 aaaactcacc acacctgcag tagacactat cgcatcaacc ttgcccgtgc gcacaaacaa
      121 agcgcgcaca ctggcgtcgt cgcggatgtc gacctgctcc atgccactgt tgcgactggc
      181 ggtaatgacg tcgtgcccta atgcatgcaa atgcgtgacc acagcctgcc ccaaaatgcc
      241 cgaagcccct attacgatga ttttcatgtc atgtcctgtg ttgtttagcg atgccgccat
      301 tattatccta agttttcatg gcataaatat gctataaatt tggaatgaat taactgtgag
      361 ttagcaatgg tggacgaaca tggacaaaat gcgtgcgttg gaagtattgg tgtgcgtggt
      421 tgaacacggc agtttcagca aggcggcaga cacgcttgac atgtcggcag tgatggtagg
      481 caaatacatc gccgccttgg aaagcgaatt gggcacccgt ttgattgaac gcagcacacg
      541 tctgcaaaaa ctcaccgccg caggcaatgc tttttaccaa caggccaaac tgactctggc
      601 acagttgcaa cacacgttta ccgtggtgga gcaattacag gccgtgccaa cgggcacact
      661 caccatcagc gccccacctt cgtttggtgc acaaaccatc gctcccatca gtgctgcctt
      721 ccaagctttg catcctacag tgaaaatcgt gctggatttg agcaatcgcc gcgtggatgt
      781 gttgcaagaa gacatagaca ttgccatccg catcggcact ttgcacaccg atgccaccgt
      841 cgccaaacac ctgtgcagct accgcatggc catcggtgcc gcacccgcgt atttacaaca
      901 cgctgggacg cctcacaact tggatgattt gtaccaacac cgcgtgttgg tacaccgttt
      961 gtggcacgac ttagaccctg agcaataccc acatttgcaa acactgcaat tgatgcaaga
     1021 gcaatcgacg tttgtcagta acgacgggtt ggccttgcgc atcgctgccg aggctggcgc
     1081 tggcatcatt gtgcagcccg aggtattgct gcaagacgca tttgccagcg gccgcttgct
     1141 gcgggtttta ccgcacatcg ccttgcccga acagccagtg catgtattgt acgcaccgca
     1201 cagcaaggcc ttgcctaagg tggcgctgtt tgtgccattt ttattgcaac acatcacaaa
     1261 atatgtgcag atggcttaat gcaagcaatc cataccgacc ttcttttgca acacggcttt
     1321 gtgcatccat ttagcacatt catcgcattg ggtttgtaaa ctgctccaat aaagaataag
     1381 cggcctgtaa atgcttacag gccgcattca tcactgcttt taacttgaac agacatcagt
     1441 gcaaactcaa actgagcaca cagcgaaagc cgcgcgctgc gtaatacgag tcggcgccat
     1501 tgtggtagac aaacaccttg tcgtaacgcc tgtcgccaaa caaggcgccg cccaaacggc
     1561 gaatcgcatc gggtgtggcc agccaggatg aggttttcaa atcgcacggc cagatgcgtt
     1621 gcaaaacttg gtattgctca gcattgatta agcttacccc catcgccgcg gcctgagcca
     1681 tggcactgcc ttgtggtggg tgtttggtgc gagccattaa agccgcctcg tcgtaacaca
     1741 ggctgcgccg tgctttggga ctttcaggtg ccgcatctgc aaaccacaac aagcccttat
     1801 cagccgcgta agccagcaca ctcggttcgc cgccgctggc ctccatttgc gccagcgacc
     1861 acaaagccgt ggcgtgttgc tgcaaagcca gtgcgatgtc tggccaagcc acatccgcga
     1921 aatgcactcg cgtgtgttca aaacggcttt gtaatacaga caatagcgcg ttggtttgcg
     1981 cagcattcat taagttacgg ctctcgtcca tgcacctctc ctaatcttca attccatcgc
     2041 ttgcgtttgc ctttaccaca caacaacgcc tgcttcatct tcatgcggcc tgtaaacaca
     2101 aaaaagcccg ataagacatc gggctttttg attcaaactg gggattaacg tttcatctga
     2161 tcaaagaaat cggcgttgtt tttcgatgat ttgattttgt cttgcaagaa ttccatcgct
     2221 tccacatcgt ccatagggtg caagaatttg cgcaacaacc acatgcgttg caattggtcg
     2281 tttggcacca acaattcttc gcggcgggtg ccagagcggt tgacgctgat ggctgggaac
     2341 acgcgttttt cggccaagcg gcgctccaaa tgcaattcca tattgccagt gcctttgaat
     2401 tcttcgtaaa tcacgtcgtc catgcggctg cctgtgtcta ccaaagcggt ggcgatgatg
     2461 gttaaagagc caccttcttc caaattacgc gcagcgccga agaagcgttt cggacggtgc
     2521 aaggcattgg catccacacc accggtcaaa atcttgccag atgcgggcac aacggtgttg
     2581 taagcacgcg ctaggcgggt aattgagtcg agcaaaatca ccacgtcttt gccatgctct
     2641 accaaacgtt tggctttttc aatcaccatt tcggccactt gtacgtggcg ttgtgcaggc
     2701 tcgtcaaagg ttgaagacac cacttcgccg cgcactgagc gagtcatttc ggtcacttct
     2761 tctggacgct cgtctatcaa caacacaatc aattccactt cagggtaatt ggtggtgatg
     2821 gcggtggcga tgttttgcaa catcacggtt ttacctgttt tcggcggcgc taccaacaaa
     2881 gcacgttgac caaaaccgat gggtgccacc aaatcaatgg cgcggctggt gacgtttttg
     2941 tcttctttgt ttttgccatc gcgttccaat ttgaattggc gcgttgggaa caagggtgtc
     3001 aggttttcaa acaggatttt gtgtttgcat ttttctgggt cgtcaccgtt gatgatgtcc
     3061 aatttcacca atgcaaaata gcgctcaccg tctttgggtg tgcgcacgct gccttcgatg
     3121 gtgtcgcctg tgtgcaaatt gaagcggcga atttggctgg ggctgacgta aatgtcatca
     3181 gggccggcca aataagaagt gtctgggctg cgcaagaagc caaagccatc aggcaagatt
     3241 tccaaagtac ctttgccagt gaagctttcc ccttttttca ccaaggcgcg cacgatggca
     3301 aaaaccaaat cttgtttgcg caagcggtta gcgttctcta tgccgtgttg ttcggccaaa
     3361 gtcagtaatt cggaaacgtg ttgtcgttgt aattcagaaa cgtgcatg
//
LOCUS       DAOMVK010000231         3401 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_8141_length_3401_cov_2.997310, whole genome shotgun sequence.
ACCESSION   DAOMVK010000231 DAOMVK010000000
VERSION     DAOMVK010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8141_length_3401_cov_2.997310"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..814)
                     /gene="cmk"
                     /locus_tag="PK856_07945"
     CDS             complement(164..814)
                     /gene="cmk"
                     /locus_tag="PK856_07945"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003774569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRL02335.1"
                     /translation="MTAVIAIDGPSASGKGTVAARVAEALGMDYLDSGALYRLTALAA
                     QTQGVAWDDEAALEPIAAQLPVAFVVGSILLNGEDVSATIRQEAIGMGASQVAKWPAV
                     RAALLERQRAFAGERGLVADGRDMGSVVFPDAKLKVFLTASAEERARRRALQLNVDLN
                     SAAYQAIVDDIKQRDWNDSQRAVAPLKQLPDAHLLDTTELSIENAVKKVLDWYRQS"
     gene            987..>2102
                     /gene="aroA"
                     /locus_tag="PK856_07950"
     CDS             987..>2102
                     /gene="aroA"
                     /locus_tag="PK856_07950"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRL02336.1"
                     /translation="MMKDFIFLPASKIQDSHVRLPGSKSISNRTLLLAALSDNVCVLR
                     DVLDSDDTKRMLEALTALGVAIQSPQAHSHQITGTSGRFPVQQADLFLGNAGTAFRPL
                     TAALAVMNGHYRLSGVPRMHERPIGDLIDALRLAGADVSYEQNTGYPPLQIAPWQDNG
                     VRQVSVKGNVSSQFLTALLMALPLTGHAYTIDVVGELISKPYIDITLKLMAQFGVHVE
                     HESHQRFHLPAAAQYHAPAELFVEGDASSASYFLAAGLISGQKITVEGVGKNSIQGDV
                     AFVDALVGIGAQVSYGDNSITVWRDAKQAIQPFDFDANHIPDAAMTLAIVAVATGQPC
                     SIRNIESWRVKETDRLSAMTTELRKLGVQVTEGQDYIS"
     assembly_gap    2103..2112
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2113..2254
                     /locus_tag="PK856_07955"
     CDS             <2113..2254
                     /locus_tag="PK856_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRL02337.1"
                     /translation="DTYDDHRMAMCFSLIALLNTPIIINEPECVNKTFPTYFQEFAKL
                     CV"
     gene            2315..2710
                     /locus_tag="PK856_07960"
     CDS             2315..2710
                     /locus_tag="PK856_07960"
                     /inference="COORDINATES: protein motif:HMM:NF016701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HRL02338.1"
                     /translation="MFHGSCLCGAVKYTILLEQLDDITLCHCHKCQKASGSAFNAVTP
                     VAAEFMQIQDEQGMLKHFESSPGVERNFCGQCGSPVFSFRPALQQYRVRIGSLDDSVN
                     IKPAQHIYYAYRAPWLIINDIAPKYPEGL"
     gene            2949..>3401
                     /locus_tag="PK856_07965"
     CDS             2949..>3401
                     /locus_tag="PK856_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HRL02339.1"
                     /translation="MTAILIILGIILLIVGILGIIYPALPGLPLMFLGALSIAYANDF
                     SYMAWGILGVILAIAIIGSLLDYVAGTLGAKFTGASKQAIWGSVIGTVIGAILSFTGM
                     FFAIFLGPLIGAALGEYIARRDLFKAGTVGLGTFMGFIAGVIAKIGCAF"
BASE COUNT          821 a          899 c          789 g          882 t
ORIGIN      
        1 ataaattctc acaaatacac cgccaaggtg tgcgggggtc tgattagtca cagttcactt
       61 gccctggcca agtaaacgat acaattcata ttgttacatg ctcttgcgtt tcttaattca
      121 aatcattgaa tcaaaaaatg gaattggcga ttatatccga atttcagctt tgtcgatacc
      181 aatccaaaac tttttttacc gcattttcaa tgcttaactc ggtggtatcg agcaaatggg
      241 catctggcaa ttgttttaaa ggtgcgaccg cacgctgact gtcattccaa tcgcgctgct
      301 taatatcatc cacaatggct tgataagccg ctgaatttaa atcaacattc aattgcaaag
      361 cgcgacgacg ggcgcgctct tctgcgctgg cggttaaaaa caccttgagc ttggcgtcgg
      421 gaaacaccac actgcccatg tcacgaccat cagccactaa accgcgctca cctgcaaacg
      481 cgcgttggcg ctctaacaaa gcggcacgca ccgcaggcca tttcgccact tgcgatgcgc
      541 ccatgcctat cgcttcttgg cgtatggtcg cgctcacgtc ttcgccattc agcaaaatac
      601 tgcctaccac aaacgccacg ggcaattgcg ctgcgatggg ttccaatgct gcttcgtcat
      661 cccaagccac gccttgtgtt tgcgctgcca acgcggttaa gcgatacaaa gcacccgaat
      721 ccaaataatc catgcccaat gcttcggcca cgcgagccgc taccgtgcct ttgcccgatg
      781 cgctggggcc atctattgca attaccgctg tcatcattcg tccttcagag gcgcaaaaac
      841 cgtgattata ggccacttat ttggtaagcg acctgtaaac attccttgat atccccgcac
      901 agtttgctgc actgcccttt cggtcaggcg ggaacttgtt tacactctag gcaattcttt
      961 acaggccgct tatttcagtt gagttgatga tgaaagattt tattttctta cccgcaagca
     1021 aaattcaaga cagccacgtg cgtcttcctg gttccaaaag catttccaat cgcactttgt
     1081 tgttggcggc cttatcagac aatgtgtgcg tcttgcgcga tgtgttggat tcagacgata
     1141 ccaaacgcat gctcgaggct ttgacggcct tgggcgtggc gattcaatcc ccacaggccc
     1201 acagccacca aatcacaggc acatcaggtc gattccctgt tcagcaagcc gatttgtttt
     1261 tgggcaacgc aggcacggcg tttcgcccgc ttaccgctgc cttggcggtg atgaacgggc
     1321 attaccgttt gtcaggcgtg cctcgcatgc acgagcgccc gattggcgac ttgattgatg
     1381 ccttgcgttt ggcgggtgcg gacgtgagct acgagcaaaa caccggctac ccaccgctgc
     1441 aaatcgcccc ttggcaagac aatggcgtgc gccaagtttc ggtaaaaggc aatgtgtcca
     1501 gccagtttct caccgcctta ctgatggcct tacctttaac gggccatgct tacaccatag
     1561 atgtggtggg cgaattgatt tccaagcctt acatcgacat caccttaaaa ctgatggcgc
     1621 aattcggtgt gcatgttgaa cacgaaagtc accaacgctt ccacttgcct gcagcagcgc
     1681 aataccacgc gcctgccgaa ttgtttgttg aaggcgatgc ctccagtgcc tcatatttct
     1741 tggcggccgg cttgatttca ggtcaaaaaa tcaccgtcga aggcgtgggc aaaaacagca
     1801 tacaaggcga tgtggcgttt gtggatgcct tggtcggcat cggtgcgcaa gtgagttatg
     1861 gcgacaacag catcaccgtg tggcgcgatg ccaaacaagc catacagcct tttgattttg
     1921 atgccaacca cattcccgat gccgccatga ctttagcaat cgtggctgtg gccacaggcc
     1981 agccttgcag catacgcaac atcgaaagct ggcgcgtgaa agaaaccgac cgcttgagcg
     2041 caatgaccac tgaattgcgc aaactcggcg tgcaagtcac cgaaggacaa gactacatca
     2101 gcnnnnnnnn nnagacactt acgacgacca ccgcatggcg atgtgttttt ctctcatcgc
     2161 cttgttgaac acacccatca tcatcaatga gccagagtgt gtgaataaga cattcccgac
     2221 ttattttcaa gaattcgcca aattgtgcgt ttaattgtca atttgctgct ttaacatcac
     2281 gaccattgca tcattttttg ctgaggagtt gaccatgttt catggaagtt gcttgtgcgg
     2341 tgcagtgaaa tacaccattc ttttagaaca gctagatgac atcaccctgt gccattgcca
     2401 caaatgtcaa aaagccagcg gctcggcatt caatgccgtc actcctgttg ccgctgaatt
     2461 catgcaaatt caagatgagc aaggcatgct caagcatttt gaatccagcc caggggttga
     2521 gcgcaatttt tgcggccaat gtggttcacc tgtgtttagc ttccgccccg cactacagca
     2581 atatcgggta cgcataggca gcttggacga cagtgtcaac atcaaacctg cccagcacat
     2641 ttactatgcc taccgagccc cgtggctcat tatcaatgac atcgccccga aatacccaga
     2701 aggcctttaa atctggcttt tattcatacc caccaagcgg cttacaggcc gcttgagtca
     2761 caaacacacc aattgcttaa tatctaccct gtattttcaa attcaattca ggggtaaata
     2821 caacacatgt ggccgacacg cacaccaagg tatttcaata ttcggccatg gttttaagct
     2881 tcttaacaat tgttatttgt cgcttgcgcc cacataataa agcccactga aacgatgaaa
     2941 gtggctttat gactgcaata ttgattattt tgggcatcat tttgttgata gtaggcattt
     3001 tgggcatcat ctacccagca ctaccgggtt tgcctttgat gtttttgggc gccttgtcca
     3061 ttgcctatgc aaatgacttc agctacatgg cttggggtat tttgggggtg attttagcaa
     3121 tcgccatcat cggctcttta ttggattatg tggcgggcac tttgggtgca aaattcactg
     3181 gagcaagcaa acaagccatt tggggcagtg tgataggcac ggtgataggg gcaatccttt
     3241 cattcacagg catgtttttc gccatcttct tagggccact gataggggcg gctttgggtg
     3301 aatacatagc cagacgagac ttattcaaag cgggcacagt aggcttgggc acgtttatgg
     3361 gctttattgc cggcgtgatt gccaaaatcg gctgcgcctt c
//
LOCUS       DAOMVK010000232         3397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_8156_length_3397_cov_3.497606, whole genome shotgun sequence.
ACCESSION   DAOMVK010000232 DAOMVK010000000
VERSION     DAOMVK010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8156_length_3397_cov_3.497606"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..>1155
                     /gene="ffh"
                     /locus_tag="PK856_07970"
     CDS             86..>1155
                     /gene="ffh"
                     /locus_tag="PK856_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRL02340.1"
                     /translation="MLDNLSSRLGQLVKTMRGHARLTEDNIKDALREVRMALLEADVA
                     LPVVKDFVAKVKEKAMGQEVLGSLTPGQAFIGVVNDSLTDLMGKQNDALNLAAVPPAI
                     ILMAGLQGAGKTTTIGKLARLLKTEQKKKVLVVSADVYRPAAIEQLRLLAEQVEVEFY
                     NSNSWEDPVQIALDAKEYAKKHFFDVLMVDTAGRLAIDEVLMEEIQKIHAAINPIETL
                     FVVDAMQGQDAVNTAKAFDNALPLTGIILTKMDGDARGGAALSVRHVTGKPIKFIGVG
                     EKVTGLEPFHPDRLSSRILGMGDVLTLIEDVQKGIDQETAAEMAKKLHKGKGFDLNDF
                     KAQIVQMRKMGGIESLMAKMPG"
     assembly_gap    1156..1165
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1166..1459
                     /locus_tag="PK856_07975"
     CDS             <1166..1459
                     /locus_tag="PK856_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003788088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRL02341.1"
                     /translation="IGEMSKHVPEGTAERAMGQVEAIINSMTAKERANPALIKASRKR
                     RIAIGSGVTVQEVNKMLKQFEQSQKVMKQFSKGGMGKMLKMAKGIKGILPGQQ"
     gene            complement(1813..3057)
                     /gene="lysA"
                     /locus_tag="PK856_07980"
     CDS             complement(1813..3057)
                     /gene="lysA"
                     /locus_tag="PK856_07980"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRL02342.1"
                     /translation="MNPYQYVNNVLHFDGVALPVLAKEYGTPLYVYSETALTAAYKAY
                     EQAFAANQPLICYAVKANGNLSVLKHFAALGSGFDIVSGGELQRVLAAGGEAGKVIFS
                     GVGKSRDEIKLALQVGVKCFNVESLPELDRINEVAAELGMTAAISLRINPDVDAQTHP
                     YISTGLKENKFGIAFVDAMGAYRHAAALPNLRIVGIDCHIGSQLIDLSPLAEALERLL
                     VLVDQLAAEGIALQHIDIGGGVGIVYNDEAQPDLQVYADTVARLLAGRPQHLVMEPGR
                     SLVGNAGLLLTKVEFVKQGEGKNFVIVDAAMNDLMRPALYQAYHAIEAVALHSDVPAV
                     LADVVGPICETGDFLAKDRELAVEEGDLLVVKSAGAYASSMASNYNTRTRAAEVMVNQ
                     GVQRLIRRREAISDLWKDEVGL"
     gene            complement(3084..3251)
                     /locus_tag="PK856_07985"
     CDS             complement(3084..3251)
                     /locus_tag="PK856_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HRL02343.1"
                     /translation="MGVLCTLLSACGFKGGLYLPKENDKQTFGAVQTGFGIDKRPNQV
                     SPTMQNNPQPF"
BASE COUNT          948 a          860 c          761 g          818 t
ORIGIN      
        1 tgtcgcaaac gcaacctgtc gtgtaaaata gaccaatttt atcaggaaca cccttgcggt
       61 gcagacaatt atctagggga tacctatgtt agacaatctt tccagccgtt tgggtcaact
      121 ggttaaaacc atgcgcggcc atgcgcgctt aaccgaagac aacatcaaag acgcattgcg
      181 tgaagtgcgc atggctttat tggaagccga tgtggccttg cctgtggtga aagactttgt
      241 ggccaaggtc aaggaaaaag ccatgggcca agaggtattg ggcagcttga ccccaggcca
      301 agctttcatt ggcgtggtga acgactcttt aactgatttg atgggcaagc aaaacgatgc
      361 cttgaatttg gccgccgttc cgcctgccat catcttgatg gccggtttgc aaggtgcggg
      421 taaaaccacc accatcggta aattggctcg cttactcaaa accgagcaaa agaaaaaagt
      481 gttggtggtt tctgccgacg tgtaccgtcc tgctgcgatt gaacaattgc gcttgttggc
      541 tgagcaagtg gaagtggaat tttacaattc caattcttgg gaagatcccg tacaaatcgc
      601 cttggatgcc aaagaatacg ccaagaaaca tttctttgat gtgttgatgg tcgataccgc
      661 gggccgcttg gcgattgatg aagtgttgat ggaagaaatc caaaaaatcc atgccgccat
      721 caatccaatt gaaaccttgt ttgtcgtgga tgcgatgcaa ggccaagatg cggttaacac
      781 agccaaagct tttgataatg ccttgccttt aaccggtatt atcttgacca aaatggacgg
      841 tgatgcacgc ggtggtgcag ccttgtctgt gcgccatgtc actggcaagc caattaaatt
      901 catcggtgtg ggtgaaaaag tcacaggttt agaacctttc caccctgacc gtttgtctag
      961 ccgcatcttg ggcatgggcg acgttttgac cttgattgaa gacgtacaaa aaggcataga
     1021 ccaagaaact gccgccgaaa tggcgaagaa attgcacaaa ggcaaaggtt ttgacttgaa
     1081 cgatttcaaa gcccaaattg tacaaatgcg caaaatgggt ggcattgagt cattgatggc
     1141 aaaaatgcca ggtcannnnn nnnnnatcgg tgaaatgtct aaacacgtgc ctgaaggcac
     1201 agctgagcgc gccatgggcc aagtagaagc catcatcaat tcgatgactg ccaaagagcg
     1261 tgccaaccca gccttgatta aagccagccg caaacgccgc atcgccatcg gctcaggtgt
     1321 gaccgtgcaa gaagtgaaca aaatgctgaa acagtttgaa caatcacaaa aagtgatgaa
     1381 gcaattcagc aaaggcggta tgggcaaaat gttgaaaatg gccaaaggca tcaaaggtat
     1441 cttgccaggt caacaataat aagacgtttc agctttatga gcagataagc ggcctgtaaa
     1501 tcgttacagg ccgcttctat ttgtttttac atctttgtgt tattacattt ccacttcaat
     1561 ctgctgcctg ctctcaaact gatttgaatg aaaaacctgc cattttgcga cttgctacaa
     1621 aatgattcag atactccact acccaatcta aacaaaccac aacatcctct aaccaagcta
     1681 aaaacttggc ttcacatacc gatacatggt ttcactggct gaatttgtgt tgtaaggcca
     1741 gatgaaaatc ttgcccatac tggcctgatt caatctccac cacgcaaacc accttatcaa
     1801 cttcagaatg gtttacaggc cgacttcatc tttccacaaa tcgctgatgg cttcacggcg
     1861 acggattaag cgctgtacgc cttggttcac catcacttca gcagcacggg tacgggtgtt
     1921 gtaattgctc gccatgctag aggcgtaagc gcctgcactt ttcaccacca acaagtcgcc
     1981 ttcctccacc gccaattcac ggtctttggc caaaaaatcg cctgtttcac atatcgggcc
     2041 caccacatcg gccaataccg caggcacatc gctgtgcagc gcaaccgctt caatggcatg
     2101 ataagcttgg tacaaagcag gtctcatcaa atcgttcatc gccgcatcca caatgacaaa
     2161 atttttgccc tcgccctgtt tcacaaattc aactttggtc aacaacaaac ccgcattgcc
     2221 caccaaagat cggcctggct ccatcaccaa atgctgcgga cggcctgcta acaagcgcgc
     2281 aaccgtatcg gcatagactt gcaaatcagg ctgagcctca tcgttgtaaa caatgcccac
     2341 accaccgcct atgtcaatgt gttgcaatgc aatgccttca gccgccaatt ggtctaccaa
     2401 taccaacaag cgctccaaag cttcggccaa tgggctcaaa tcaatcaatt gtgaaccgat
     2461 gtggcaatca atgcctacga tgcgcaaatt cggcaacgct gccgcgtggc ggtaggcacc
     2521 catggcatcg acaaatgcga tgccaaattt gttctctttc aaaccagtag agatataagg
     2581 atgggtttgc gcatcaacat cgggattgat gcgcaaggaa atggctgccg tcatgcctaa
     2641 ctcagccgcc acctcattga tgcggtctaa ttcaggcaaa gattccacat tgaaacactt
     2701 cactcctact tgcagcgcca atttgatttc atcgcggctc ttgcccacgc cagaaaaaat
     2761 cactttacca gcctcaccac ctgcggccaa cacgcgttgc aattcgccac cagacacgat
     2821 gtcaaaaccc gagcccaaag cagcaaaatg ctttaacacg ctcaaattgc cattggcttt
     2881 gaccgcgtaa caaatcaagg gttgattggc ggcaaaagcc tgctcatatg ccttatatgc
     2941 tgccgttaat gcggtttcgc tgtagacata caaaggtgtg ccgtattctt tggccaaaac
     3001 aggcaaagcc acgccgtcaa agtgcagaac attgtttacg tattggtatg ggttcatggt
     3061 gtgtttcgtc gtctaaggtt gaattaaaaa ggctgcgggt tgttttgcat ggtgggggaa
     3121 acctgattcg ggcgcttgtc tatgccaaaa cccgtttgca cagcaccaaa ggtttgctta
     3181 tcgttttcct taggcaaata taagccgcct ttgaaaccac aagcactgag taatgtgcac
     3241 agcacaccca tccacaaaat tttcacaccc tactcccgat aatattgatt gccagcttgc
     3301 gcttattgcc cgtaaacgaa cattgttttc aaggaaaact cgtggcaaga tgggcaatct
     3361 taacaaaata caccgtcaga caccatgagc atgaatg
//
LOCUS       DAOMVK010000233         3391 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_8171_length_3391_cov_2.283273, whole genome shotgun sequence.
ACCESSION   DAOMVK010000233 DAOMVK010000000
VERSION     DAOMVK010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8171_length_3391_cov_2.283273"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PK856_07990"
     CDS             complement(<1..245)
                     /locus_tag="PK856_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02344.1"
                     /translation="MKKWLWLSCLLFLTACGFQLRGVGLHDTLLPYHKWALEDAGSMK
                     NALKYELKRRQNVEVVDAADSEAILRVLSVNTDKRTQS"
     gene            complement(257..1684)
                     /gene="gatB"
                     /locus_tag="PK856_07995"
     CDS             complement(257..1684)
                     /gene="gatB"
                     /locus_tag="PK856_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRL02345.1"
                     /translation="MTWETVIGLEIHVQMNTQSKIFSGASTAFGADANTHTSVVDLAM
                     PGALPVMNRAVIEKGIKLGLALKATINQTNIFDRKNYFYPDLPKGYQISQMDYPIVEF
                     GKLDIVVGDEVKTINVTRAHMEEDAGKSVHDAVQGYTGIDLNRAGTPLLEVVSEPEMR
                     NAQEAVAYAKAMHDLVTWLEICDGNMAEGSFRIDANVSVRPVGQEAFGTRCEIKNLNS
                     FRFLEAAINYEVERQIELIEDGGKVIQQTRLWDPDKGETRAMRSKEDAHDYRYFPDPD
                     LLPVIISDEQLARVKAEMPELPAEMAERFVAAYAITDYDARLLTTSKLTADYFETAAK
                     TSNEGKLVANWMNGELAASLNRAEKTLAESPISAERLAGLVKKISDGTLSSKLAKKVF
                     EALWESDLSAEAIIERDGLQQINDTGAIEKMIEEVLAANPKSVEEFKAGKEKALNALV
                     GQVMKASRGKANPAQVQDLMKAKLV"
     gene            complement(1748..>3391)
                     /locus_tag="PK856_08000"
     CDS             complement(1748..>3391)
                     /locus_tag="PK856_08000"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRL02346.1"
                     /translation="NPLVQARAYSMEVINMLQADKALQQVDGLHKGRLKIPYGHGVVL
                     SHIPRKNMFAALTAEAREKVLPDHLVLYQEDLHDEVDAETIQEQLWGMFPYQFGEALS
                     AAEVDRVRWHLFPEIRIDAMALQGQLFAEESLAETVETAFKRPVNMENDTAASIAESV
                     AIPDLIKVMDMQQEQLARNLGSGHRVIHGVAGSGKTLILAYRCQYLAMKSTKPILVMC
                     FNVTLAAKLRVMMVSKGLGDKVQAVHFHDWCAEQLRQYGIAVMAGEGKIYDRQVATVL
                     QAVADGRIPKEQYGAILIDEGHDFAAEWLQVLTQMLDREEESLLLLYDDAQSIYRRGG
                     LDFSLSSVGIKAQGRTSVLRLNYRNSREILAFAAAFASDYWQNQELQDEDRIPVIAPE
                     AAGILGPEPMVYVCEDFVAEADKAAKCIQSWQKKGLALNKIAILAYTKEQAKQVSKIL
                     SEQGINAQLLLDGRSKKAYQPEDNSVAIMTLHSSKGLEFSHVVVMGVGQMGKVTDRVN
                     DDARVLYVGLTRAQRVLVVTGSGKGRFIQQLVQQVAAVS"
BASE COUNT          819 a          929 c          733 g          910 t
ORIGIN      
        1 gattgggtgc gtttgtcagt attaacgctc aagacacgca aaatggcttc tgaatcggcc
       61 gcatccacca cttccacatt ttggcgacgc tttaattcat acttgagcgc atttttcatg
      121 cttcccgcat cttccaaagc ccatttgtgg taaggcaaca aggtatcgtg tagcccgaca
      181 ccccgcagct ggaacccaca agccgtaagg aaaagcaaac aactgagcca cagccatttt
      241 ttcatgtgtt cgtcctttaa accaatttgg ctttcatcaa atcttgcact tgagcagggt
      301 tggctttgcc acgactggct ttcatgactt ggcctaccaa agcattcaag gctttttctt
      361 tacccgcttt gaattcttct accgattttg gattggcagc caacacttct tcaatcattt
      421 tttcaatcgc gcccgtgtca ttgatttgtt gcaaaccatc gcgctcaatg atggcttcgg
      481 cgctcaaatc gctttcccac aaggcttcaa atactttttt cgccaattta ctcgacaaag
      541 tgccatcgct gatttttttc accaagcccg ccaagcgctc ggctgaaatc ggtgattccg
      601 ccaaggtttt ttcggcacgg ttcaagcttg cagccaattc accgttcatc cagttcgcca
      661 ccaacttgcc ttcatttgac gttttggcag cggtttcaaa ataatcggcc gtcagttttg
      721 aagtggtgag caaacgcgca tcgtaatcgg tgatggcata ggcggcgaca aagcgctctg
      781 ccatttcagc aggcaattct ggcatttcgg ctttcacgcg cgccaattgc tcgtctgaaa
      841 tgatgacggg taacaagtct ggatcaggga aatagcggta atcgtgcgcg tcttctttag
      901 aacgcatggc acgggtttca cctttatcag gatcccacaa acgggtttgt tggatgactt
      961 tgccaccgtc ttcaatcaat tcgatttgac gttctacttc gtaattgatg gcggcttcta
     1021 agaagcggaa agagttcagg tttttgattt cacaacgcgt accaaacgct tcttggccca
     1081 cagggcgtac cgatacgttg gcatcgatgc gaaacgatcc ttcggccatg ttgccatcgc
     1141 aaatttccaa ccatgtcacc aaatcgtgca tggctttggc ataagccacc gcttcttgtg
     1201 cattgcgcat ttcaggctca gacaccactt ccaacaaagg cgtacccgca cggttcaaat
     1261 caatgcctgt atagccttgc accgcatcgt gtaccgattt gcccgcatct tcttccatgt
     1321 gcgcacgggt cacgttgatg gttttgactt catcgcctac cacaatgtcc aatttaccaa
     1381 attcgactat cgggtaatcc atttggctga tttggtagcc tttgggcaag tcggggtaaa
     1441 aataattttt gcggtcaaaa atattggttt ggttgatggt ggctttcaaa gccaagccca
     1501 atttgatgcc tttttcaatc accgcacggt tcatcaccgg caaagcgcca ggcatggcca
     1561 aatccaccac cgaggtgtgg gtgttggcat cggcaccaaa cgcagtggat gcaccgctga
     1621 aaattttcga ttgcgtgttc atttggacgt ggatttccag tccaattacg gtttcccaag
     1681 tcatgtttta actctcttct tgcggtttac aggcggcctg taaaccaggt attgcttaaa
     1741 aataaatcta acttacggcg gcaacttgct gcacaagctg ctgaataaat ctgcccttac
     1801 ctgaacctgt caccaccaaa acccgttgtg cgcgggtcaa gcccacgtac agcacacggg
     1861 catcatcatt gacgcggtcg gtgactttgc ccatttgccc cacgcccatc accaccacgt
     1921 gtgaaaattc caggcctttg gaggaatgca aagtcatgat ggcaacgctg ttgtcttcgg
     1981 gctgataggc ttttttactg cggccatcaa gcaacaattg cgcattgatg ccttgctctg
     2041 acaagatttt gctgacctgc ttcgcctgct ctttggtata cgccaaaatc gcaatcttat
     2101 tcaaagccaa accttttttc tgccacgatt gaatgcattt ggcggcttta tccgcctcag
     2161 cgacaaaatc ctcacaaaca taaaccatag gctctgggcc taaaatgccc gcagcttctg
     2221 gtgcaatcac gggaatgcgg tcttcatctt gcagctcttg attttgccaa tagtcactgg
     2281 caaatgctgc ggcaaacgcc aaaatctcgc ggctgttgcg gtaattcaag cgcaacacag
     2341 aagtacggcc ttgcgctttg attccaacgc tcgacagtga aaaatccaat ccgccacggc
     2401 ggtaaatgga ttgggcatcg tcataaagta acaataaaga ttcttcttca cggtcaagca
     2461 tttgcgtcaa tacttgcagc cattctgcag cgaaatcatg cccttcatca atgagaatgg
     2521 ccccatattg ctctttgggt atgcgcccat cggcaacggc ttgtaatacg gtggcaactt
     2581 ggcgatcata gattttgcct tcgcctgcca tcaccgcaat cccatactgg cgcaactgct
     2641 cggcacacca atcatgaaaa tgcacagcct gaaccttgtc acccaaacct ttgctgacca
     2701 tcattacccg cagcttggcc gccagcgtca cattaaaaca catcaccaag atgggcttgg
     2761 tcgacttcat cgccaaatac tgacagcgat aagccaaaat cagtgtttta cccgagcccg
     2821 ccaccccatg tatcacacgg tggccactgc ccagattgcg tgccaactgc tcttgctgca
     2881 tgtccatgac tttaatcaaa tcagggatgg ccacagactc ggcaatgctt gccgcagtgt
     2941 cattttccat gtttacaggc cgcttgaatg cagtctctac ggtctctgca agcgactctt
     3001 ctgcaaataa ttgaccttgc aaagccatgg catcaatgcg aatttctgga aacaaatgcc
     3061 agcgcacacg gtcgacttcc gccgcactca aagcctcacc aaattgatag ggaaacatgc
     3121 cccacaattg ttcttgtatg gtttcagcat ccacctcgtc gtgcaaatct tcttggtaca
     3181 acaccaaatg gtcgggcaag accttttctc gtgcctcagc cgtcaatgcc gcaaacatgt
     3241 ttttgcgcgg aatgtgactg agcaccacgc catgtccata cggtattttc aagcggcctt
     3301 tgtgtaagcc atcaacctgc tgcaaagcct tatcggcctg taacatattg atgacttcca
     3361 tggaataagc ccgtgcttgt accaatggat t
//
LOCUS       DAOMVK010000234         3365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_8261_length_3365_cov_2.245619, whole genome shotgun sequence.
ACCESSION   DAOMVK010000234 DAOMVK010000000
VERSION     DAOMVK010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8261_length_3365_cov_2.245619"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /gene="ptsP"
                     /locus_tag="PK856_08005"
     CDS             <1..1057
                     /gene="ptsP"
                     /locus_tag="PK856_08005"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003747937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HRL02347.1"
                     /translation="IIDPDEKILAEYRKRAKIYRNQKRQLNKLKNTAATTADDVNIQI
                     FANIEAPSDITTAHKFSIDGVGLFRSEFLYLNRDTLPTEDEQYEIYLDAVKRMNGKPL
                     TIRTVDLGVDKNPRWFSHGDALNPALGLTGIRLCLAEPIMFRTQMRAVLRAAVHGPIR
                     LLWPMIGSLSELRQCHAHLDLAKQQLSDRGMAFQADIPVGCMIEIPSAALMVTSLLRQ
                     VDFLSVGTNDLIQYTLAVDRSDDTVSYLYQPAHPAVLKLLTHILRSAIKAGKPVSVCG
                     EMAGDDRYTRLLLGMGLRQFSMHPSNILRVKNMIVHSNTDEVEKQVWRILRNDDPNKV
                     DDLLKKLNQLETQKTAA"
     gene            complement(1087..1608)
                     /locus_tag="PK856_08010"
     CDS             complement(1087..1608)
                     /locus_tag="PK856_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02348.1"
                     /translation="MENSTATIATKPKARRSRVSKAFWGLIGLMAIVSIGLISMAVVA
                     MNKYTYATEAVEKPKDPGVEIMDLGGGTSKIVSGNTLPAKPLDVDESVVDGGTAGKAT
                     ATMAEQKDAGGALVDKVAVKPVTAPKKQQVAVADAGEDSPKPKASVSANKQEKTPVSK
                     QHKESNKQMDNLF"
     gene            complement(1728..2339)
                     /locus_tag="PK856_08015"
     CDS             complement(1728..2339)
                     /locus_tag="PK856_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HRL02349.1"
                     /translation="MKKALTAALIGLSLTQLVSGCAVLAVGGIAAGAASALDRRSTGA
                     QADDQMMELQVSNTVNAYLKEQSGKDVYVNVKTVSYNRNVLLLGMVPNQEAKDFAERV
                     ARSQTAVNNVYNHISIGQARDFNAAQDTWLTSKIRTMLLKPQGYNPNQVKITTFNGVT
                     YAQGVLTPDEQAAVSAQISTTLGVQKVVTLYETFVPTASPASP"
     gene            complement(2361..2951)
                     /locus_tag="PK856_08020"
     CDS             complement(2361..2951)
                     /locus_tag="PK856_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HRL02350.1"
                     /translation="MNLSDRVFDHFAESIKAKQDNQELLAEATAAAIELMFAALVNDG
                     KVLACGNGGSAADAQHFAAELVGRFEMERMGLAGIALTTDTSALTAIANDYDFNRIFS
                     KQVEALGRSGDVLLAISTSGNSANIIAAIEAAHERDMRVVALTGKDGGKIAHILQDQD
                     ILLNVAHPRTARIQEIHILLIHAICDGIDQLLMGNV"
     gene            complement(2948..3301)
                     /locus_tag="PK856_08025"
     CDS             complement(2948..3301)
                     /locus_tag="PK856_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRL02351.1"
                     /translation="MRLQHPTGLAAEERAAQFLSQQGCRIVARNWHCKSGEIDLVAWD
                     GAVCVFVEVRLRSSEQFGGAAASITASKQRKLLLSAQTYLQAEHSSEPECRFDAVLIN
                     GEGHIEWLKNILECK"
BASE COUNT          817 a          920 c          767 g          861 t
ORIGIN      
        1 catcatcgac cccgatgaga aaattttggc cgaataccgc aaacgcgcca aaatttaccg
       61 caaccaaaag cgccagctca acaaactcaa aaacaccgcc gccaccactg ctgacgatgt
      121 caacattcag atttttgcca atattgaagc acccagcgac attaccaccg cgcacaaatt
      181 ctccatagat ggcgtgggct tgttccgcag tgaattttta tacctgaacc gcgacaccct
      241 gcccaccgaa gacgagcaat acgaaatcta tttggatgcc gtgaagcgca tgaacggcaa
      301 accgctgacc atacgcaccg tggatttggg cgtggacaaa aacccacgct ggttcagcca
      361 cggcgacgca ctcaatcctg ctttgggctt aacaggcata cgcttgtgtt tggccgagcc
      421 catcatgttc cgcacccaaa tgcgtgcagt gctgcgcgca gccgtgcatg gtcccattcg
      481 tttattgtgg ccgatgattg gctctttgag cgaattgcgc caatgccatg cgcatttgga
      541 tttggccaaa cagcaattgt ccgaccgcgg tatggcattc caagcggata ttcccgttgg
      601 ctgtatgatt gaaatcccat ccgcggcctt gatggtgacc agcttgctgc gccaagtgga
      661 tttcctgtct gtcggtacca atgacttgat tcaatacacc ttggcagtag accgcagtga
      721 cgataccgtc agctatttgt accaacctgc tcaccctgcg gtgttaaaac tgctcaccca
      781 cattttgcgc agcgccatca aagcaggcaa acccgtatcg gtgtgcggcg aaatggccgg
      841 tgacgaccgc tatacccgct tattgctcgg catgggctta cgccaatttt ccatgcaccc
      901 atcgaatatt ttgcgggtaa aaaacatgat agtgcacagc aataccgatg aggttgaaaa
      961 acaggtttgg cgcattttgc gcaatgacga ccccaacaaa gtcgatgact tactgaagaa
     1021 actcaatcag ttagaaacgc aaaaaaccgc tgcctaagca gcggtttttt tgtttacagg
     1081 ccgcatttaa aacaaattgt ccatttgctt attagactct ttgtgctgct tgctcacggg
     1141 ggttttctct tgtttgttag cagaaacaga ggcttttggt ttaggactgt cttcgcccgc
     1201 atcggcaacc gccacttgct gtttcttagg tgcggtcaca ggcttcacag ccactttgtc
     1261 taccaatgcg ccgcccgcat ctttttgctc ggccatggtt gcggtggctt tgcccgcagt
     1321 gccaccgtct accaccgact cgtccacatc caaaggtttg gctggcaaag tattgccact
     1381 gacaatttta ctggtaccac cgcccaaatc catgatttcc acaccaggat ctttgggctt
     1441 ttcaaccgct tcagtggcgt aagtgtattt gttcatggcg accacggcca tgctgatgag
     1501 tccaatggac acgattgcca tcaagcctat caggccccaa aatgctttac tgacgcgaga
     1561 gcgtcgtgct ttgggtttgg tggcgatggt tgcggtgcta ttttccataa tattacaatc
     1621 caaagtgtct gtatggccct gattcatttg atgaaatggc atcgacacat taacacaaat
     1681 actttaaaac gtctgtattc ggccgcaaaa cggccgaatt aacagactta tggtgatgca
     1741 ggactggccg ttggtacaaa tgtttcgtac aaagtcacca ctttttgcac ccctaacgtc
     1801 gtgctgattt gcgcgctgac cgccgcttgc tcatcaggtg tcaacacacc ttgtgcatag
     1861 gtaacgccat tgaaagtggt gattttcact tggttggggt tatagccttg tggctttaac
     1921 aacatggtac ggattttaga agtcaaccat gtgtcttgcg ccgcattaaa gtcacgcgcc
     1981 tgaccgatgc tgatgtggtt gtacacattg ttcaccgcgg tttgtgaacg ggccacgcgt
     2041 tccgcaaagt ctttggcttc ttgattcggc accatgccta acaacaacac attgcggttg
     2101 tagctgacgg ttttgacatt aacatacaca tctttgccgc tttgctcttt caaataagca
     2161 ttgacggtat tgctcacttg caattccatc atctgatcgt ctgcctgcgc gcccgtactg
     2221 cgtctgtcta atgcagatgc ggctccagcg gcgatgccac ccacggccaa aacggcgcaa
     2281 ccactgacca attgcgttaa actcaaaccg attaatgctg ctgttaatgc ttttttcatg
     2341 caatagtcct tacaggccgc ttacacattg cccattaaaa gttgatcaat accatcacag
     2401 attgcgtgaa ttaacaaaat atggatttct tgaatgcgcg cggtgcgtgg gtgcgccaca
     2461 ttcaataaga tgtcttggtc ttgcaagatg tgggcgattt taccgccgtc tttaccggtt
     2521 aaagccacca cgcgcatgtc gcgttcatgg gccgcttcaa tcgctgcaat gatgttggcc
     2581 gaattgcccg aagtcgaaat cgccaacaac acatcaccac tgcgacccaa agcctcaacc
     2641 tgcttggaga aaatgcggtt aaagtcgtaa tcgttggcaa tcgcggttaa agccgaggtg
     2701 tcggtggtca aagcgatgcc cgccaagccc atgcgttcca tttcaaaacg acccaccaat
     2761 tcggcagcaa aatgctgggc atcggcagcc gagccaccat tgccgcaagc caaaaccttg
     2821 ccatcgttta ccaaggcggc aaacatcaat tcgatggcag cggcagtggc ttctgccaat
     2881 aattcttgat tgtcttgttt tgccttaatg ctttctgcaa aatggtcaaa tacgcgatcg
     2941 cttaaattca tttacattcc aatatgtttt tgagccactc gatgtggcct tcgccattga
     3001 ttaacaccgc atcaaatcgg cattcaggct cgcttgagtg ttcggcttgc agataggttt
     3061 gcgcacttaa cagcaattta cgttgcttac tggccgtaat cgatgccgcg gcgccgccaa
     3121 actgctctga gctgcgcagg cgcacctcga caaacacgca cacagcccca tcccaagcca
     3181 ccaaatcgat ttcaccgctc ttacaatgcc aattgcgcgc cacaatgcgg caaccctgct
     3241 ggcttaaaaa ctgtgctgcg cgctcttctg ctgccaaacc agtcggatgt tgtaagcgca
     3301 tgccactctc cgcatatgat agtatcacac cattgaaatg cccaatgaga ctacagtgtt
     3361 acaac
//
LOCUS       DAOMVK010000235         3363 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_8268_length_3363_cov_3.773881, whole genome shotgun sequence.
ACCESSION   DAOMVK010000235 DAOMVK010000000
VERSION     DAOMVK010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8268_length_3363_cov_3.773881"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="PK856_08030"
     CDS             <1..503
                     /locus_tag="PK856_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRL02352.1"
                     /translation="NLVFILLPPISIWAFFLAYVGAAIARGLFVGVGVLAVCAFFGLT
                     WPVSVTWTILFAIMGCAIMGTLGLLAGIVAEKFDQMAVFQNFLIMPLTFLSGVFYSIN
                     SLPEFWRTISHFNPVFYMIDGFRYGFFGHSDVSPWLSLSVVTICWLATSIGILMLLKS
                     GYKLRH"
     gene            519..767
                     /locus_tag="PK856_08035"
     CDS             519..767
                     /locus_tag="PK856_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009116308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HRL02353.1"
                     /translation="MLTPEQVQAMIANVLPCEHIAVEGDGHHFFAHIVSNEFEGKARL
                     ARHRLVKDGLADVIASNELHALSIATAATPSEWAAKQH"
     gene            831..2093
                     /gene="murA"
                     /locus_tag="PK856_08040"
     CDS             831..2093
                     /gene="murA"
                     /locus_tag="PK856_08040"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRL02354.1"
                     /translation="MDKLRIHANGPLNGEITISGAKNAALPLMCAALLTKETLRLTNV
                     PMLRDVKTTHKLLEGMGATVSTDGVSVFDINCGTLNELCAPYDLVKTMRASILVLGPT
                     LAHFGEAQVSLPGGCAIGSRPVDQHLKGLEAMGADIVIEHGYVKAKLKAERLQGTRIV
                     MDMVTVGGTENLMMAACLASGTTVLENAALEPEVVDLGECLIKMGAKIYGLGTPTITI
                     EGVESMHGAEHAVVADRIEAGTFLCAVAMTGGKVLLKNAAPKTMTAVLDKLVEAGAHI
                     EAGDDWILIDMQHRPRPVNIRTLPHPAFPTDMQAQFMAMNAVADGASKVTETIFENRF
                     MHVPELNRMGAQISTEGNTAMMQGVAQLSGATVMGTDLRASASLVIAGLVASGETIIE
                     RIYHLDRGYENIEAKLCGVGANIVRISE"
     gene            2152..2787
                     /locus_tag="PK856_08045"
     CDS             2152..2787
                     /locus_tag="PK856_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRL02355.1"
                     /translation="MKYLTLGLSTALVLGLSACQPSSSTPASASHASATQAVAGKIAT
                     LNGSDISAEGIGADFQLLNQDGKTVSLADFKGKWVILSFGYTRCPDVCPTTLLDLSQV
                     MTLLGADADKVQVVMVSVDPSRDTPAVMKDYVSLFNERFVGLTAQDDAAMAPVLKSWK
                     IAMSKVPIQDGHYSVDHSAGMYVLDPNGKAKLYLPYATKPEHIAADLKALM"
     gene            2796..>3363
                     /locus_tag="PK856_08050"
     CDS             2796..>3363
                     /locus_tag="PK856_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRL02356.1"
                     /translation="MFESSAEIPYQHITFDHGIVVSVKRIDLVHPHIAGNKFYKLKYN
                     LQAAREQGKTLLISFGGAYSNHIHALAHAAHEYGFQAIGIIRGQELAEQALNPTLQDA
                     ANLGMRLEFISRSDYRQKHTPEFLQAVLSRHPNAYIIPEGGCNDLAIQGCAEILSDAD
                     KRDFEVICCAVGTGGTIAGIINASSDAQY"
BASE COUNT          784 a          911 c          868 g          800 t
ORIGIN      
        1 gcaatttggt gtttattctg ttgccaccga tttcgatttg ggcgtttttc ctcgcctatg
       61 tcggcgcggc gattgcgcgt ggcctgtttg tcggcgtggg cgtgttggct gtgtgtgcgt
      121 ttttcggtct gacttggcct gtgtctgtga cgtggacgat tttgtttgcc atcatgggct
      181 gcgccatcat gggcactttg ggcttgcttg caggcatcgt ggcggaaaaa ttcgaccaaa
      241 tggcggtgtt ccaaaacttc ctcatcatgc ctttaacctt tttatcgggc gtgttttact
      301 ccatcaacag cttgcccgaa ttttggcgca ccatcagcca tttcaacccc gttttctata
      361 tgatagatgg ttttcgctac ggctttttcg gccacagtga tgtgtcgccg tggttgtcgc
      421 tgtctgtggt cactatttgt tggcttgcca cctctatcgg catcttaatg ctgttaaaat
      481 ctggctataa attacgtcat taatcataaa ggtctgcaat gttaactccg gaacaagttc
      541 aagccatgat agccaatgtg ttgccatgcg aacacatcgc tgttgaaggc gatggccacc
      601 atttttttgc ccacattgtt tccaatgaat ttgaaggcaa agcccgtttg gcccgccacc
      661 gcttggtcaa agacggcttg gccgatgtca tcgccagcaa tgaattgcac gctttgtcca
      721 tcgccaccgc cgctacccca agcgaatggg ctgcgaaaca acactgatac cccgcgatca
      781 atgcggcctg taaatggttt acaggccgct tatacagaaa gacccccact gtggataaac
      841 tacgaattca cgccaatggc ccactcaatg gcgaaatcac catttctggc gccaaaaacg
      901 ctgccttacc tttgatgtgt gcggcgttgc tcaccaaaga aaccctgcgt ttgaccaatg
      961 taccgatgct gcgcgacgtg aaaaccacgc acaaattgct cgaaggcatg ggtgcaaccg
     1021 tgtctaccga cggtgtcagc gtgttcgaca tcaattgcgg cactttgaac gaattgtgcg
     1081 cgccgtatga cttggtcaaa accatgcgcg cgtccatctt ggtattgggc ccgactttgg
     1141 cgcactttgg cgaagcccaa gtgagtttgc ctggcggttg cgccatcggt tcacgccctg
     1201 tcgatcagca tttgaaaggc ttggaagcca tgggtgccga catcgtgatt gaacacggct
     1261 atgtcaaagc caagctcaaa gccgagcgct tacaaggcac acgcattgtg atggacatgg
     1321 tcaccgtggg cggcactgaa aacctgatga tggcagcgtg tttggcttct ggcaccaccg
     1381 tgttggaaaa cgcggctttg gaaccagaag tggtggattt gggcgaatgc ctgattaaaa
     1441 tgggcgcaaa aatctacggc ttgggcacgc ctaccatcac catcgaaggc gtggaaagca
     1501 tgcacggcgc agaacacgcg gtggtggccg accgtattga agcgggcact ttcttgtgcg
     1561 ctgtggcgat gacaggcggt aaagtcttgc tcaaaaatgc ggcccccaaa accatgacgg
     1621 ccgtactcga caaattggtg gaagcaggcg cgcacattga agcaggcgac gattggattt
     1681 tgatcgacat gcaacaccgc ccgcgccctg tgaacattcg caccttgcca cacccagcct
     1741 tccctaccga catgcaagcg cagtttatgg cgatgaatgc ggtggccgat ggcgcgtcta
     1801 aagtgaccga aaccattttt gaaaaccgct tcatgcacgt gccagaactc aatcgcatgg
     1861 gcgcgcaaat ctctaccgag ggcaacactg ccatgatgca aggcgtggcg caactgtctg
     1921 gtgccacggt gatgggcaca gacttgcgcg cgtcagccag cttggtgatt gcaggcttgg
     1981 tggccagcgg tgaaaccatc atcgagcgca tttaccacct agaccgtggt tacgaaaaca
     2041 tcgaagccaa gctctgtggc gtcggtgcca atattgttcg catcagcgaa taaacccgta
     2101 cgagcggctt gtaagcgttt acaggccgct tgtttgcatg aaagctttga catgaaatac
     2161 ctgaccttgg gtttgagcac agcattggtg ctgggcttga gcgcttgcca accttcctca
     2221 tccacccctg cttccgcctc acacgccagt gccactcaag ccgtagcagg caaaatcgcc
     2281 accctcaatg gcagcgacat cagcgccgaa ggcataggtg cggattttca attgctcaat
     2341 caagatggca aaaccgtgtc tttggccgat tttaaaggca aatgggtgat tttatcgttt
     2401 ggttataccc gttgccctga tgtttgcccc accactttat tggatttatc gcaagtgatg
     2461 acgctgctcg gcgctgatgc tgacaaagtg caagtggtga tggtgtcggt agacccaagt
     2521 cgcgacacgc ctgcggtgat gaaagactat gtgagcttgt tcaatgagcg ttttgtcggc
     2581 ctcaccgccc aagacgatgc cgccatggcg cctgtattga aatcgtggaa aatcgccatg
     2641 agcaaagtgc cgattcaaga cggccattac agcgtagacc acagcgcagg catgtatgtg
     2701 ctcgacccca atggcaaggc caagctctat ctgccttacg ccaccaaacc cgaacacatt
     2761 gctgctgacc tcaaagcctt gatgtaagcc gcaacatgtt tgaatcgtct gcagaaattc
     2821 cctaccaaca cattacgttt gaccacggca tagtcgtcag cgtcaaacgc attgatttgg
     2881 tgcacccgca catcgcaggc aacaagtttt acaagctcaa atacaactta caggccgcac
     2941 gagagcaagg caaaaccttg ctcatcagct ttggcggcgc gtattccaac cacattcatg
     3001 ccttggcgca cgccgcacac gaatacggtt ttcaagccat aggcatcatc cgtggacaag
     3061 aattggccga gcaagcctta aaccccactt tgcaagacgc ggccaatttg ggcatgcgcc
     3121 ttgaattcat cagccgcagc gattaccgcc aaaaacacac gcctgaattc ttacaggccg
     3181 tgttaagccg ccatccaaat gcttacatca ttcccgaagg tggttgcaac gatttggcca
     3241 tacaaggctg tgcagaaatt ttgtccgatg cagacaaacg cgattttgag gtgatttgct
     3301 gcgccgtagg cacgggcggc accatcgcag gcatcatcaa tgccagctca gacgcgcaat
     3361 aca
//
LOCUS       DAOMVK010000236         3358 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_8282_length_3358_cov_2.713291, whole genome shotgun sequence.
ACCESSION   DAOMVK010000236 DAOMVK010000000
VERSION     DAOMVK010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8282_length_3358_cov_2.713291"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="PK856_08055"
     CDS             complement(<1..1027)
                     /locus_tag="PK856_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRL02357.1"
                     /translation="MTQIISKINSRSDEFKTNAEAMQAQLRDLHEVTNTIHQGGGEAA
                     RVKHEGRGKLLARDRIDSLIDRGSNFLEIGQLAAWNMYSGDIASAGMVAGIGQIQGQQ
                     CVIVANDATVKGGTYYPMTVKKHLRAQEIAEQNHLPCVYLVDSGGAFLPLQDEVFPDR
                     DHFGRIFYNQAQMSAKGIPQIAVVMGSCTAGGAYVPAMSDETVIVRDQGTIFLGGPPL
                     VKAATGEVVSAEDLGGGDVHTRISGVADYLAENDRHALGLARNIVSNLNRKKQVDMTL
                     TEVEEPVYAAEELYGIVPTDTRKPYDVREIIARIVDGSKFDEFKARFGTTLICGFARL
                     YGMPVGII"
     gene            complement(1041..2213)
                     /locus_tag="PK856_08060"
     CDS             complement(1041..2213)
                     /locus_tag="PK856_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HRL02358.1"
                     /translation="MNIQTIDFGLGDDINMLRDAVQDFANNEIAPRAAQVDADNEFPA
                     DLWRKFGDMGLLGVTVGEEYGGSNMGYVAHMVAMEEISRASAAIGLSYGAHSNLCVNQ
                     INRNGSAEQKQRFLPKLVSGEFVGALAMSEPNAGSDVVSMQLKAEKKGDRYVLNGSKM
                     WITNGGDADVMVVYAKTDPAAGPKGITAFLVEKGTKGFSHGNHLDKLGMRGSNTYPIF
                     FDDVEIPEENVMGGVGNGVKVLMSGLDYERAVLSAGPLGIMDACMDVVVPYLHDRKQF
                     GQAIGEFQLMQGKLADMYSTWLACKALVYTVGKACDQADHARSLRKDAASAILYAAEK
                     ATWMAGEAIQTLGGNGYINEFPTGRLWRDAKLYEIGAGTSEIRRMLIGRELFNETA"
     gene            2379..2990
                     /locus_tag="PK856_08065"
     CDS             2379..2990
                     /locus_tag="PK856_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRL02359.1"
                     /translation="MATTYKRSSMMQARMEQNRHTIFQAARGLIAVGGFKEAQMAAIA
                     EQAGVSTGLIYRYFSNKTQLMVEILTDAVSKEIVILNDIAKRPETAEVNLHAAVTAFV
                     RRALTGPQLAYAFMAEPVDPQVEAERILCKQRFANVFKEILVAGMGKEEFNIDDVETA
                     AACVVGAMTEAVISPITPTTPTTHDKHRVATTIADFCVRAVCR"
     gene            complement(3108..>3358)
                     /locus_tag="PK856_08070"
     CDS             complement(3108..>3358)
                     /locus_tag="PK856_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HRL02360.1"
                     /translation="LAYLASMRLNCLIIDCAHPPQNETPRNHNDFNMVCYLVDVLRCD
                     EVILTHIGHEMDAWAEQNSAELNAPFVLARDGMQRLFE"
BASE COUNT          823 a          916 c          798 g          821 t
ORIGIN      
        1 cgatgatgcc cacgggcatg ccatacaagc gcgcaaagcc acaaatcaaa gtggtgccaa
       61 aacgggcttt gaattcatca aatttcgagc catcgacaat gcgggcaatg atttcgcgca
      121 catcgtaagg cttgcgggta tcggtaggca caatgccata caattcttca gcggcgtaaa
      181 caggctcttc cacttcggtc agcgtcatgt ccacttgttt cttgcgattg aggttgctga
      241 cgatgttgcg cgccaagccc aatgcatgtc tgtcgttttc cgccaaataa tccgccacac
      301 ctgagatgcg cgtgtgcaca tcgccaccgc ccaaatcctc tgctgacacc acctcacctg
      361 tggcggcttt caccaacggt gggccaccca agaatatggt gccttggtcg cgcacaatca
      421 cggtttcgtc agacatcgca ggcacatacg caccacccgc ggtacagctc cccatcacca
      481 ccgcgatttg cgggatgcct ttggccgaca tttgcgcttg gttgtagaaa atgcggccga
      541 aatggtcacg gtctgggaac acttcgtctt gcaaaggcaa gaaagcacca cctgaatcca
      601 ccaaatacac gcaaggcaag tggttttgtt cggcgatttc ttgcgcacgc aagtgttttt
      661 tcaccgtcat ggggtaatac gtgccgcctt ttaccgtggc atcgttggcc acaatcacac
      721 attgttggcc ttgaatttgg cctatgcctg ccaccatgcc agcagaagcg atgtcgccgc
      781 tgtacatatt ccaagccgcc aattggccaa tttccaagaa attgctgccg cggtcgatga
      841 ggctgtctat gcggtcacgg gccaacaatt tgccacgccc ttcgtgtttc acacgcgccg
      901 cttcgccgcc gccttggtgg atggtattag tgacttcgtg caagtcgcgc aattgtgctt
      961 gcatggcttc agcattggtt ttgaattcgt cgctacggct gttgatttta gaaatgattt
     1021 gcgtcatgtg tggctcccaa ttacgccgtt tcattgaaca attcgcggcc gatcaacatg
     1081 cggcgaattt cagaagtgcc tgcaccgatt tcgtacaatt tggcatcgcg ccacaagcgg
     1141 cctgtaggga attcattgat gtagccattg ccacccaagg tttgtatcgc ttcgcctgcc
     1201 atccaagtgg ctttttcagc cgcatacaaa atcgcgctgg ccgcgtcttt gcgcaaagaa
     1261 cgcgcatgat cggcttgatc gcaagcctta cccacggtat acaccaaggc tttacacgcc
     1321 aaccaagtgg aatacatgtc ggccaatttg ccttgcatta attggaattc gccaatggct
     1381 tgaccaaatt gtttgcggtc gtgcaaatac ggcaccacca cgtccataca cgcatccatg
     1441 atgcccaaag gccctgcgct caataccgca cgctcataat ccaagcccga catcaacact
     1501 ttcacgccat tgcccacacc acccatcacg ttttcttcag ggatttccac gtcatcaaag
     1561 aaaatcgggt aagtattgga gccacgcatg cccaatttgt ccaaatggtt gccgtggcta
     1621 aagcctttgg tgcctttttc caccaagaag gcagtaatgc ctttcgggcc tgccgcaggg
     1681 tcggttttgg catacaccac catcacatcg gcatcgccac cgttggtaat ccacattttt
     1741 gagccattga gcacatagcg gtcgcctttt ttctcagctt tcaactgcat actcaccaca
     1801 tccgaacccg cattgggctc agacatggcc aaagcaccga caaactcacc cgacaccaac
     1861 ttaggcaaaa agcgttgttt ttgctcggcg ctgccattgc ggttgatttg gttcacacac
     1921 aaattggaat gcgcaccata agacaaacct atcgctgcag aagcacgaga gatttcctcc
     1981 atggccacca tgtgcgccac ataacccatg ttgctgccgc cgtactcttc acctacggtc
     2041 acaccgagta agcccatgtc gccaaacttg cgccacaagt cggcaggaaa ttcattgtca
     2101 gcatccactt gggcggcgcg tggggcaatt tcattgttgg caaagtcttg cactgcatcg
     2161 cgcaacatgt tgatgtcatc gcccaagcca aagtctatgg tctgtatgtt cattggtgtc
     2221 tctcccagtc tcggtgtttg tttgtgcttc tcagtttttg aaactcgttt acaatggaat
     2281 atacaacgaa aaaaataaaa agtgaacaat aattcataaa atgatttaaa attcaccaac
     2341 agaataagaa aacgtcttaa acagaaagct tgaatgccat ggcaaccact tacaaacgtt
     2401 catcgatgat gcaagcccgt atggagcaaa accgacacac catttttcaa gccgcacgtg
     2461 gtttgattgc cgtaggggga ttcaaagagg cgcaaatggc ggcgattgcc gagcaagcgg
     2521 gggtttctac aggtttgatt taccgctatt tttccaacaa gacccaattg atggtggaaa
     2581 ttctgaccga tgcggtgagc aaagaaatcg tgattttgaa cgacattgcc aagcggcctg
     2641 aaacggctga ggtcaatttg catgcggccg tgaccgcgtt tgtgcgccgc gctttaacag
     2701 ggccgcaatt ggcgtatgcg tttatggctg agcctgttga cccgcaagtg gaagccgagc
     2761 gcattttgtg caaacagcgc tttgccaatg tgtttaaaga aatcttggtc gctggtatgg
     2821 gcaaggagga attcaacatc gacgatgtgg aaaccgcggc ggcgtgtgtg gtgggcgcaa
     2881 tgactgaggc cgtcatcagc ccgattacgc ccaccacacc caccacccac gacaaacacc
     2941 gtgtggccac gacgattgcc gatttttgtg tgcgggcggt gtgccgttaa taccgtgtat
     3001 tgacacaatt taaagaacag aaatgcttct aaaatggttt acaggccgca taatttgcac
     3061 catgcggcct gtaaacatta gcaaacgtgt ttattcaaac acaccactca ttcaaataac
     3121 ctttgcatgc catcgcgtgc caacacaaac ggcgcgttca attcggcact gttttgctct
     3181 gcccatgcgt ccatttcatg accgatgtgg gtgagtatca cttcatcaca gcgcaatacg
     3241 tccaccaaat aacacaccat gttgaaatca ttgtggttgc gcggggtttc attttgcggc
     3301 gggtgggcgc agtctatgat gaggcaattt aaacgcatgc tggccaaata ggccaagg
//
LOCUS       DAOMVK010000237         3351 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_8311_length_3351_cov_3.691141, whole genome shotgun sequence.
ACCESSION   DAOMVK010000237 DAOMVK010000000
VERSION     DAOMVK010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8311_length_3351_cov_3.691141"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..424)
                     /locus_tag="PK856_08075"
     CDS             complement(125..424)
                     /locus_tag="PK856_08075"
                     /inference="COORDINATES: protein motif:HMM:NF013939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HRL02361.1"
                     /translation="MQAHECQTLAQARTEIDRLNSEILALLAQRQHYVERVADLKTDI
                     SEVPAPERRAAVLRQVAQEASDLGMSSEVAVAVFDAMIAAFIALELKQFKDLNQA"
     gene            complement(437..1492)
                     /gene="tal"
                     /locus_tag="PK856_08080"
     CDS             complement(437..1492)
                     /gene="tal"
                     /locus_tag="PK856_08080"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HRL02362.1"
                     /translation="MTKLQQVAALGQQIWLDSLSRSLIQSGALAEKLADGVMGITSNP
                     AIFQQAFQNDALYTEELAALKQQDLDAKSRYETMAIADVRNACLVCLPVYQSNNGQAG
                     MVSLEVSPSLAHDTAGTVAEGKRLWHAINQPNTMIKVPATAAGIEALRELVAAGINVN
                     MTLIFSRAVLADILQAHAQGLQQRLGQGLPIDKIAVVASFFLSRVDTALDISLPEHLQ
                     GKVALALARSAYADWQDFVANEAWQALANQGAHAVQLLWASTGTKNPAYPDTLYVDQL
                     IGANTVNTVPDKTLSAFIDHGTASATLDGHKDTSLHILSEVAALGIDIEALALRLRDD
                     GLQQFEDAFAKILDITA"
     gene            complement(1529..2071)
                     /locus_tag="PK856_08085"
     CDS             complement(1529..2071)
                     /locus_tag="PK856_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003778157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HRL02363.1"
                     /translation="MNNIRPFLNTHPQTDAAIFIDPSCVIIGDVVLGADSSVWPMAVI
                     RGDVNYIRVGERSNVQDLAMLHVSHKNAAKPDGSPLIIGDDVTVGHQAMLHGCRIGNR
                     VLVGMKTTILDDAIIEDDVMIGAGSLVPARKRLESGYLYVGSPVKQVRKLTEDEIAFL
                     AYSAKHYVKVATQHQQSSQT"
     gene            2476..>3351
                     /locus_tag="PK856_08090"
     CDS             2476..>3351
                     /locus_tag="PK856_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRL02364.1"
                     /translation="MAKFAQTFILSAIASVTLAACGGAETKPAEPAASSKPVASASGA
                     APAADGAPKRVAITAIVEHPALDAVRKGVEEQLAADGYKVGTNLTIDFQSAQGNTATA
                     GQIAKKFAGDKPDVIVAIATPSAQSVVAATRDVPVVFAAVTDPVAAKLVTSWEPSGTN
                     VTGVSDELPLEPQIDLMKKLVPNVKAVGYVYSPGEVNSTVILEKLKTQLKTQGIEVIG
                     APAQRTTDISTAANSLAGKVQMIYTTTDNNVVSGYESLYKVAAANKIPLLASDTDTVK
                     RGASAALGVNYHDMGM"
BASE COUNT          807 a          789 c          863 g          892 t
ORIGIN      
        1 tttaatttgc taattggaac agttctcatt tgttttcaat tgcttaaatg aaaataaatg
       61 ctgtttgtat tgtgaaaagc ggcctgtaaa tgctttacag gccgcttgca gtgcttggtt
      121 tggattaggc ttgatttaag tctttgaatt gctttaattc cagcgcgata aaggcagcaa
      181 tcatggcatc aaataccgcc accgccacct cgctgctcat gcccaaatcg ctggcttctt
      241 gagctacttg gcgcaaaacc gcggcgcgac gctcgggtgc gggcacttcg ctgatgtcgg
      301 ttttgaggtc ggccacgcgt tcaacatagt gttgcctttg tgctaacaaa gccaaaattt
      361 cgctgttgag gcggtcgatt tcggtgcggg cctgcgccag tgtttggcat tcatgtgctt
      421 gcatgggcgg ctttcgttac gcggtaatgt ccaaaatttt ggcaaacgcg tcttcaaatt
      481 gttgcaaacc atcgtcgcgc aaacgcaagg ccaaggcttc gatgtctatg cccaaagccg
      541 ccacttcgct caaaatgtgc agcgatgtgt ctttgtggcc atccaaagtg gcgctcgccg
      601 tgccgtggtc tatgaaggcg cttaaggttt tgtcgggcac ggtgttgacc gtgttggcac
      661 cgatgagttg gtctacatac aaggtgtcag ggtaggcggg gttttttgtg cctgtggagg
      721 cccacagcaa ttgcaccgca tgtgcgcctt ggtttgccaa tgcttgccat gcctcattgg
      781 ccacaaaatc ttgccaatcg gcataggcag agcgcgccaa ggccaaagca accttgcctt
      841 gtaagtgttc aggcaagctg atgtcgagtg cggtatcgac acgcgacaag aagaagctgg
      901 cgaccacagc aattttatca ataggcaagc cttgacctaa acgctgttgt aagccttgtg
      961 catgggcttg caaaatatcg gccaatacgg cgcgtgagaa gattaaggtc atgttgacat
     1021 tgatgccagc ggccaccaat tcgcgcaaag cttctatgcc tgcggcagta gccggcactt
     1081 taatcatggt attgggttgg ttgatggcat gccacaagcg tttgccctcg gccaccgtgc
     1141 cagcggtatc gtgtgccaag ctgggtgaca cttccaagct caccatgcct gcttggccat
     1201 tgttcgattg ataaaccggc aaacacacca agcaagcatt gcgcacatcg gcaatggcca
     1261 tggtttcata ccggcttttg gcatcgaggt cttgctgttt taaggccgcc aattcttcgg
     1321 tatacaaggc atcgttttga aacgcttgct gaaaaatagc agggttggag gtgatgccca
     1381 tcacgccatc ggccaatttt tcggccaatg cgcccgattg aatcaatgag cgagacaagc
     1441 tgtctagcca aatttgttgc cctaatgccg ccacttgttg caattttgtc atgtttcttc
     1501 cttgagtcct gctgtatcgg cctgtgtgtc aggtttgcga tgattgttgg tgttgtgtgg
     1561 ccactttaac atagtgtttg gcagaatagg ctaagaacgc tatttcgtct tcagtgagct
     1621 tgcgcacttg tttgacgggt gagccgacat acaaatagcc gctctccaaa cgcttgcgtg
     1681 caggcaccaa gctgccagcg cctatcatca catcgtcttc aatgatggca tcgtctaaga
     1741 tggtggtttt catgcctacc aacacacgat tgccaatacg gcagccgtgt aacatggctt
     1801 ggtggcccac cgtgacatca tcgccgatga tgaggggaga gccatcgggc ttggcggcat
     1861 ttttgtgcga cacatgcagc atggccaagt cttgcacatt gctgcgttcg cccacgcgga
     1921 tgtagttcac atcgccacgt atcaccgcca taggccacac cgagctgtcg gcgcccaaca
     1981 cgacatcacc aatgatgacg cagctggggt ctatgaatat tgcggcatcg gtttgtggat
     2041 gggtgtttaa aaaaggtcgt atgttgttca tcatcattgt tttcatgtta agtgagctta
     2101 caggccgctt gataaggcgg agatttatgg ccgctgtgcc gaacagattt gcgtgacatt
     2161 aaattaagca ccataaaaca gcaaataaac gataagtatg attcttgcaa ttcatttgag
     2221 cttattagca tattatatta tttatcatta aatcattggt tgattttatt tttatcctga
     2281 tatttaataa acattaataa taatttacat ggcaaaccct ctgtgtgggt atgtgtcagc
     2341 tttgctgaat ttactttaga ataagccagt tttcagtaac agcatgacat tgtgttgaat
     2401 gcacgcaaaa taaaataatc gtgcaaacct gacaccatgc tgataatcat caacgagaaa
     2461 tagaaggaca agaacgtggc caaatttgcg caaacattca ttttatctgc cattgccagt
     2521 gtgactttgg ctgcttgcgg cggcgctgaa accaaacctg ctgaaccagc agcaagcagc
     2581 aaacctgtag ctagcgccag tggtgctgcc cctgctgctg atggtgcacc gaaacgcgtg
     2641 gccattaccg ctattgtgga gcatcctgct ttggatgcgg tgcgcaaagg tgtggaagag
     2701 caattggcgg ctgatggcta caaagttggt accaacttga ccattgattt ccaaagcgct
     2761 caaggcaata ctgctaccgc aggtcaaatt gccaaaaaat tcgctggcga caagcctgac
     2821 gtgattgtgg cgattgcgac gcctagtgcc cagtctgtgg tggcggcaac gcgtgatgtg
     2881 cctgtggtat ttgctgcggt aaccgaccct gtggcagcca aattggtgac ttcttgggag
     2941 ccttctggca ctaatgtaac aggtgtgtct gacgaattac cattggagcc gcaaatcgat
     3001 ttgatgaaaa aattggttcc taatgtgaaa gccgtgggtt atgtgtacag cccaggtgaa
     3061 gtgaattcaa ccgtgatttt ggaaaaattg aagacccagt tgaaaaccca aggcattgaa
     3121 gtgataggcg cacctgccca gcgcacaacc gacatttcta ccgcagccaa cagcttggct
     3181 ggcaaagtgc aaatgatata caccaccacc gacaacaatg tggtgtctgg ttatgagtct
     3241 ttgtacaaag tggctgctgc caacaagatt cctttgttgg cttctgatac cgataccgtg
     3301 aagcgcggtg cttctgcggc attgggcgtg aattaccacg acatgggcat g
//
LOCUS       DAOMVK010000238         3333 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_8370_length_3333_cov_2.937157, whole genome shotgun sequence.
ACCESSION   DAOMVK010000238 DAOMVK010000000
VERSION     DAOMVK010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8370_length_3333_cov_2.937157"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(280..813)
                     /locus_tag="PK856_08095"
     CDS             complement(280..813)
                     /locus_tag="PK856_08095"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HRL02365.1"
                     /translation="MRWREQSVSIGYAQGKVADGDTVRGVNVKYNYEFSNQWGVLAST
                     TYMQGDSSVGANQLPDISPDKWGEASRDTKYFSVMAGPSYRINNNINVYGVVGIARTS
                     AKGQAQWLNHEGGKYVHRGDFSVKDTSTGLGYGAGVQINPTTNIVVDLAYEGARSSNA
                     LENKNLNAFNVGLGYRF"
     gene            complement(735..>914)
                     /locus_tag="PK856_08100"
     CDS             complement(735..>914)
                     /locus_tag="PK856_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02366.1"
                     /translation="TMSLFTQQNHGIKGNCHEEVISVSVCFLPMSHASCDGVNSRYPL
                     GMHKGKLLMETLCAV"
     assembly_gap    915..924
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1297..1306
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1307..1831)
                     /locus_tag="PK856_08105"
     CDS             complement(<1307..1831)
                     /locus_tag="PK856_08105"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRL02367.1"
                     /translation="MFETSLSASQVTQALYETFVMLGWSLLIGGLWGLVQGTVMVLIR
                     DNGVRPNVALFRILTPIINTLRSVPFIILLIALIPITKFIVGTSIGTMAAVVPLSLYI
                     GPYIGRLVETSLLEVGSGIIETARSLGASTWQIVWHFLLPEARQSLVLNLTTATIGLL
                     GATTMAGAVGAGGIG"
     gene            complement(1824..2855)
                     /locus_tag="PK856_08110"
     CDS             complement(1824..2855)
                     /locus_tag="PK856_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRL02368.1"
                     /translation="MVRFEHVEKTYLKNGRSVEALKHINLDIPAGSVFGIIGHSGAGK
                     STLLRLINGLESPSSGRLSVLGQDLSAVSAQELKRLQKQIGMVFQHFNLLDSKTVFEN
                     VALPLRLNKVGNQEVEERVSALLDYVGLADKAQMRPHQLSGGQKQRVGIARALVNRPQ
                     ILLCDEATSALDPQTTASILGLLKKINQEQGITIVLITHEMEVIQKIAQQVAVMSEGE
                     IVEAGSTAAIFQHPQQRVTQEFIATVIQKDFPLKLVQQLNTAHSQDSQIVRLEFLQQS
                     AQQTVMNDMLLTHTVKANILFANMIEIDGQVIGSMFVELTGRLAERSVALQYLTERNV
                     VVDQGVAYV"
     gene            3066..>3333
                     /locus_tag="PK856_08115"
     CDS             3066..>3333
                     /locus_tag="PK856_08115"
                     /inference="COORDINATES: protein motif:HMM:NF025587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HRL02369.1"
                     /translation="MTHSTDPTTTLPILDLRLLEAADTKAAFLQNLQDLARNTGFFYL
                     IGHGISAARIQEIENLSRQFFAQDQASKDALSMTNSPHFRGYSRV"
BASE COUNT          904 a          862 c          666 g          881 t
ORIGIN      
        1 gaaactttat tttttcttcc attgattgct tagtcgaaat atcaatgaga tacaacacgt
       61 tcagtatgaa actttattac tctcatacaa ctgatgtatg agagtaacaa agtgccatac
      121 tagattgctc aaaaatccac acactccatg ccacccaagc atgtatgtgt ggatttttta
      181 tggtttactt tagccgagcg caccattcac acatgcattc aaagacatat gctcataaaa
      241 aaagccagca attgctggct actgatccca aattcaattt taaaaccgat aacccaaacc
      301 aacattgaat gcatttaaat tcttattttc caaagcattg ctagaacgag caccttcata
      361 agccaaatca acaacaatgt tggtagttgg attgatttgc acacctgcgc cgtatcccaa
      421 accagttgac gtgtctttta ctgaaaaatc accacgatga acatatttac caccctcgtg
      481 gttaagccat tgcgcttgcc ctttggctga ggtgcgggcg atgcccacca caccatacac
      541 attgatatta ttgttaatgc gataactagg gcccgccatc acggagaaat acttggtgtc
      601 tctagatgct tcgccccact tatctgggct gatatcaggc aattgattgg cacccacact
      661 gctgtcaccc tgcatataag tggtactggc caatacgccc cactgattcg aaaattcata
      721 attgtatttc acattcacac cgcgcacagt gtctccatca gcaactttcc cttgtgcata
      781 cccaatggat accgactgtt cacgccatcg caactggcgt gagacatagg caagaaacaa
      841 acagaaacag agatgacttc ttcatggcaa ttccccttaa taccatggtt ttgttgtgta
      901 aataacgaca tggtnnnnnn nnnnaataaa ttcattgaag ttaaagcact atcaatgcca
      961 atttatattt cgatattcat ttcctagccc attttcatcg ccaaatctgt tcatgtcaca
     1021 tcggttctga taattgaatc accgcagacc gcgccataca tcacaaaagg gatttacctt
     1081 aagtttgcct tgtcacattt acaggccgct taatttactt aagcggcctg taatgccttt
     1141 gaaacacttt taaactacgg atggctcatt tgagcattga tatatgaagt ttacaggctg
     1201 catggctgca taggtttaag ccaaacgctt ggcgatgaat tcacccgtaa actgtatcgc
     1261 ttgcacgatg gcaatcaaaa tcaacacggt aatcacnnnn nnnnnngccg atgccgcctg
     1321 cacccactgc gcccgccatg gtggtagcgc ccaataagcc tatggtggcg gtggtgaggt
     1381 ttaagaccaa agattggcgc gcctcgggca acagaaaatg ccaaacaatt tgccatgtgg
     1441 atgcgcccaa gctgcgggcg gtttcgatga tgccgctgcc cacttccaac agcgaagttt
     1501 ccaccaagcg accgatataa gggccgatat acaagctcaa aggcaccact gctgccattg
     1561 tgccgatgct ggtaccaaca atgaatttgg tgatgggtat cagtgcaatc agcaaaatga
     1621 tgaagggaac agagcgcaaa gtattgatga tgggcgttaa gatgcgaaac aaagccacat
     1681 ttgggcgcac gccattgtcg cgtatcaaca ccatgaccgt gccttgcacc aaaccccaca
     1741 agccgccgat gagcagcgac caacccaaca tcacaaaggt ttcatacaaa gcctgtgtga
     1801 cttggcttgc gcttaaactg gtttcaaaca tatgccactc cttggtctac caccacattt
     1861 ctctcggtca aatactgcaa ggccacgctg cgctcggcca agcggcctgt aagctctaca
     1921 aacatgctgc ctatgacttg gccatcgatt tctatcatgt tagcgaacaa aatattcgcc
     1981 ttcaccgtgt gcgtcagcaa catgtcgttc atcactgttt gctgcgccga ttgctgtaaa
     2041 aattccaagc gcacaatctg gctgtcttga ctgtgtgcgg tattcaattg ctgcaccagc
     2101 ttcaaaggaa agtctttttg tatcaccgtg gcgatgaatt cctgtgtcac tcgctgttgt
     2161 gggtgttgaa aaatcgccgc agtgctgcct gcctccacaa tctcgccctc actcattacc
     2221 gccacttgtt gggcaatttt ttgtatcact tccatctcat gggtgatgag cacaatggtg
     2281 atgccttgct cttgattgat tttcttgagc aagcccaaaa tcgaagccgt ggtttgtggg
     2341 tctagcgccg aagtcgcctc gtcgcacaac agtatttgcg gtcgattcac caaggctcgg
     2401 gcaataccaa cgcgttgttt ttgcccacct gaaagttgat gcggacgcat ttgcgctttg
     2461 tctgccaagc ccacatagtc caacaaggcg cttacccgtt cttctacttc ctgattgccc
     2521 accttgttca agcgcagtgg caaagccacg ttttcaaaca cggttttgct gtcgagcaag
     2581 ttgaagtgtt gaaacaccat gccaatttgc ttttgtaagc gtttcaattc ttgcgcagac
     2641 accgcgctca agtcttgccc caacacgctc aagcggcctg aactcgggct ttccaagcca
     2701 ttaatcagtc gcaacaaagt ggatttgccc gcaccactgt gaccaatgat gccaaacaca
     2761 ctgcctgctg gaatgtccaa attgatgtgt ttcaaagcct cgacgctgcg cccatttttc
     2821 agatatgtct tctcgacgtg ttcaaaccgc accatatgcg ccacccactg caagtaaaaa
     2881 tcgcactgta gcaaaggtca attgatttaa aaaagaatta aaaattcatt tcaaataacc
     2941 taaaggcata aatacatatt acttatggtt attaaattat cgtttaatat tattttttaa
     3001 attttttcgt tgcgattatg ctgtttgcac acacaaaacc gctgacaagc acacggagaa
     3061 atcagatgac tcacagcacc gacccaacca ccaccctgcc gattttggat ttgcgcttac
     3121 ttgaagccgc ggataccaaa gccgccttct tgcaaaactt gcaagacttg gcgcgcaata
     3181 caggtttttt ttatttgata ggccatggca tcagcgctgc gcgcatccaa gaaattgaaa
     3241 acctgagccg ccaatttttt gctcaagacc aagccagcaa agacgcgctg tccatgacca
     3301 actcaccgca ttttcgcggc tattcccgcg tca
//
LOCUS       DAOMVK010000239         3316 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_8424_length_3316_cov_2.527446, whole genome shotgun sequence.
ACCESSION   DAOMVK010000239 DAOMVK010000000
VERSION     DAOMVK010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8424_length_3316_cov_2.527446"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1352)
                     /locus_tag="PK856_08120"
     CDS             complement(<1..1352)
                     /locus_tag="PK856_08120"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRL02370.1"
                     /translation="MEHSYPLVETIVGGLVFAFILGMVAQRLRMPPLIGYLFAGIIVG
                     PYGPDIPGMQGSLELSHQLAELGVILLMFGVGLHFSIKDLMAVRKVALPGAIVQIGFA
                     TILGMGLASIMGWSWGAGLVFGLALSCASTVVLMAALERWHIKDSNRGRIAIGWLIVE
                     DIAMVFVLVLIPALSGLLSGQGESMTFTEVALLVAKTCFWMILFVVVMMVGGNKFIPW
                     SLKKVVRTGNKDLFRLGVLAIALGCAIVANYLFHVSFALGAFFAGMILSETEMNHAAE
                     EESSAVRDFFTVLFFVSVGMLFNPEVLTTVSGIVLVLSTLFIIIIGKSVAAYMIVKMF
                     GYPKTTALTIAVSLAQIGEFSFMIASLGLSYKILEPAQNDAILAAAMISIILNPFLFV
                     ALNQYKEKKNRVPAHEAHVPTIDGTKTNSAEADGETSILDAKPRAKVAAPIKPLSIVF
                     "
     gene            complement(1572..2387)
                     /locus_tag="PK856_08125"
     CDS             complement(1572..2387)
                     /locus_tag="PK856_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRL02371.1"
                     /translation="MGLDATTWLVMLCAGAFAGFWAGLLGVGGGLIIVPIMVWILSHQ
                     GVGPEYSQHLAIGTSFAVMVFTSFSSVLAQHKREAVRWDLVWKLAPWMVIGTFVGASL
                     AKYVPGLMLQSVFVVFAFVIAAQTFMDFKPKASRQMPGTAGSVASGGFIGMLSSWVGI
                     GGGSLSVPFMLFCNVPIVNAVGTSAAFGWPIALAGCIGYLVNGWAVPGLPTGAAGFVY
                     LPALIGLAIGTVTFAPIGVKVAHKLPPRKLKLAFAMMLTITACQMLWKILHTI"
     gene            complement(2448..>3316)
                     /locus_tag="PK856_08130"
     CDS             complement(2448..>3316)
                     /locus_tag="PK856_08130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HRL02372.1"
                     /translation="CGDAAPLALMLAAYSAEQTNDDAAKQGYLQRLAALPESMQLSRY
                     LLEAESALERYDYEAAQMSIDAARKLNPGLTRLLQLELRMAVDQKDAMKVLRLTEQLA
                     KAGALSTTELQQYQWVAYRQLLAQCHDAKALKTCLKRIPDADQKGGLSVEIAERFQQL
                     GLYAQAAKWAKANYPLKRDVALLNVLFESSANLSDKEQQQAFEAADGWLKTYREDTDL
                     LLALGEAAYQRQLWGKAQSYLEASLSQHPSIQAHLALAKVFKATEQNALAEQHQAAAL
                     ALVEQAGSWDEA"
BASE COUNT         1011 a          907 c          652 g          746 t
ORIGIN      
        1 tcaaacacaa tagataaagg tttgattggt gcagccacct tggctcttgg cttggcatcc
       61 aaaattgagg tttcaccatc ggcctcagca ctgttggtct ttgtgccatc aatagtgggc
      121 acgtgcgctt catgggcagg cacacggttt ttcttttcct tgtattggtt caaagccaca
      181 aacaagaatg gattcaagat gatggaaatc atcgctgcgg ccaaaatggc atcgttttgt
      241 gcaggctcca aaattttata gctcaaaccc aaagaggcga tcatgaaaga gaattcaccg
      301 atttgtgcca aagaaaccgc gatggtcaaa gctgtggttt tgggataacc aaacattttc
      361 acaatcatgt aagcagcaac agacttgcct atgatgatga tgaataaggt acttaacacc
      421 aacacaatac cagacacagt cgtcaatacc tcgggattaa acaacatccc cactgacaca
      481 aagaacaaaa cggtgaaaaa gtctcgaacc gcgctggatt cttcttcagc cgcatggttc
      541 atctcagttt cgctcaaaat catacctgca aaaaatgccc ccaatgcgaa cgaaacatga
      601 aacaaataat tggcaacgat ggcgcagccc aaagcgatgg ccaacacacc caaacgaaat
      661 aagtctttat tgcctgtgcg caccactttt ttcaatgacc atgggatgaa cttattgcca
      721 cctaccatca ttaccaccac aaacaaaatc atccaaaaac acgttttggc aaccagtaaa
      781 gccacttcag tgaaagtcat gctttcacct tgaccagaca acaaacctga tagcgctgga
      841 atcaacacca acacgaacac catggcgatg tcttcaacta tcagccaacc tatggcaatg
      901 cggcctcgat tggaatcttt gatgtgccaa cgctccaaag ccgccatcaa taccacggta
      961 ctggcacatg acagcgccaa accaaatacc aaacctgcgc cccaactcca gcccatgatg
     1021 gaagccaggc ccatgcccaa tatggttgca aagccaattt gcacaatggc gccaggcagc
     1081 gcgactttac gcaccgccat caaatcttta atggaaaaat gcagacccac accaaacatc
     1141 agcaaaatca cacccaattc agccaattga tgtgagagtt ccaagctgcc ttgcatgcct
     1201 ggaatgtctg ggccataagg gccaacaatg ataccggcaa acagatagcc gattaatggc
     1261 ggcatgcgca agcgttgcgc aaccatcccc aaaatgaaag caaaaaccaa gccacccaca
     1321 atggtttcaa caagtggata agaatgctcc atggcactcc tttatacaga atagagttaa
     1381 cagatgacga tagatatcga gagagatgcc cacacccact gatgactagg cattaaagac
     1441 acacatacaa aaagccgaat atgaagtgtg ttcaccagag cgtggaacca cttaaatata
     1501 tcggctttta tcgacttttg gcaaccgttt tatgccaatc atcttatttt taacgcaaat
     1561 tgctaaattt attaaattgt atgcaatatt ttccacagca tttggcaagc ggtaatggta
     1621 agcatcatcg caaaagccaa cttcagtttg cgtggaggca gtttgtgcgc gacttttacc
     1681 cctattggcg caaatgtgac cgtgcctatg gccaaaccta tcaacgcagg caagtacaca
     1741 aaacctgccg cacccgttgg caatcctgga acagcccaac catttaccaa atagcctata
     1801 caacctgcca gcgcaatagg ccaaccaaat gcagccgatg tgcctactgc attcacaatc
     1861 ggcacattac aaaacaacat gaatggtacc gacaaagaac caccaccaat acccacccaa
     1921 gaggaaagca tgccgataaa accaccagat gccacagaac ctgcagttcc tggcatttgt
     1981 cgactggctt tgggtttaaa atccatgaat gtttgtgcgg cgataacaaa cgcaaacacc
     2041 acaaaaaccg attgcaacat caaacctggc acatacttgg ccaaactggc acccacaaaa
     2101 gtacctatca ccatccacgg tgccaacttc caaaccaaat cccaacgcac tgcctcacgt
     2161 ttgtgctgtg ccagaacgct agaaaagctg gtaaatacca tcactgcaaa cgaagtgcca
     2221 atcgccaaat gttggctgta ttcaggccct accccttgat gtgaaagtat ccacaccatg
     2281 atgggtacaa tgattaagcc tccgccaacc cccagcaaac ctgcccaaaa acctgcaaat
     2341 gcacctgcac ataacatcac caaccatgtg gtggcatcta aacccataaa catttgtgac
     2401 acgcctataa caaaaaaatg gccgcataag cggcctgtaa acccatatta cgcttcatcc
     2461 caactgcctg cttgctccac caaagccaat gcagccgctt ggtgttgctc agccaaagca
     2521 ttttgttctg tcgccttaaa tactttcgcc aaagccaaat gcgcttgtat cgatggatgt
     2581 tgagacaagc tggcttccaa atagctttgc gccttgcccc ataactggcg ttggtatgcg
     2641 gcctcaccca aggccaacag taagtcagtg tcttcacggt aggtcttcaa ccaaccatcg
     2701 gcagcttcaa aagcctgttg ctgttcttta tcagacaaat tggcagatga ttcaaacaac
     2761 acgtttaaca aagccacatc acgtttcaga gggtaattgg ctttggccca tttggccgct
     2821 tgagcataca gacccaattg ctgaaaacgc tcagcaattt caacgctcag accacctttt
     2881 tggtcggcat cagggatgcg tttcaaacag gttttcaagg ctttggcatc atgacactgc
     2941 gccaacaatt gacggtacgc cacccattgg tattgttgca attcggttgt gctcaatgcc
     3001 cctgccttgg ccaattgctc ggtcaaacgc aagactttca tcgcatcttt ctgatccaca
     3061 gccatgcgta attccaattg caacaagcgg gtcaaaccag gattgagttt gcgtgctgca
     3121 tcgattgaca tttgtgcggc ttcgtaatcg taacgttcca acgcactttc tgcttcaagc
     3181 aaatagcgcg ataactgcat ggactcaggc aaggcagcaa gacgctgcaa ataaccttgt
     3241 ttggccgcat catcattggt ttgctcggca ctatatgccg ccaacatcaa ggccaatggc
     3301 gccgcatcac cacact
//
LOCUS       DAOMVK010000240         3313 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_8435_length_3313_cov_3.682934, whole genome shotgun sequence.
ACCESSION   DAOMVK010000240 DAOMVK010000000
VERSION     DAOMVK010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8435_length_3313_cov_3.682934"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="PK856_08135"
     CDS             <1..773
                     /locus_tag="PK856_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HRL02373.1"
                     /translation="GKGGVGKSTTTTNLAIALSKMGARVGVLDADIYGPSQPTMLGVA
                     HLKPEQRNGKFIPVVSDGGIQTMSIGFMIDTDQAVVWRGPMVSQALQQLLFQSQWDDV
                     DYLFIDLPPGTGDIQLTLSQKIPVTASVIVTTPQDIALLDARKAVDMFAKVNIPILGI
                     VENMAMHVCSNCGHAEAIFGSDGGKALADKLNVPLLAQLPLSLPIREAMDAGQGAALH
                     SKETAIADLYQQAAWQVALKVADMGRDFSGRFPKIVVE"
     gene            936..1475
                     /locus_tag="PK856_08140"
     CDS             936..1475
                     /locus_tag="PK856_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HRL02374.1"
                     /translation="MALKATIYKANINVANMDEHVYGDFSLTMARHPSETEERLMVRL
                     LAYCRFATPDTEGLEFTKDLFETGEPALWQHDLTGMLTQWIEVGLPDEDKVRKASARC
                     ERVAVVAYGSTAPDWYQKSSKLKTLGNVSVWTLSTASTAAVQALCSRGMDLQLNIMDG
                     EWTLISDDAQAIIEWEQWQ"
     gene            complement(1554..2144)
                     /locus_tag="PK856_08145"
     CDS             complement(1554..2144)
                     /locus_tag="PK856_08145"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRL02375.1"
                     /translation="MVSWWFVGLVGGILFTPGPTNTLLATAGLQRGWWQATRLIPFEL
                     VGYVLAISVWGYGMAQMTAHLPQLPAIMKTLSAVYLAFLAMRLWQHAQASDSESTGRI
                     AAPNLFLATLLNPKALLFATAVFPMETWRSWQGYFTIMAEFGLLVGMAACAWIALGAS
                     MRQAKQTWLRPEYLQRLAACVLSVFAFGLLGSIWSI"
     gene            2634..>3313
                     /locus_tag="PK856_08150"
     CDS             2634..>3313
                     /locus_tag="PK856_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO family protein"
                     /protein_id="HRL02376.1"
                     /translation="MNTQPSHLLSLPEHHYSTVAVQPLHATNLLAYNEALAAELGLPA
                     AAALNEDERLSLAGSLENPPQAPIATVYSGHQFGIWAGQLGDGRAMLLGDITDPNGQT
                     WELQLKGAGITPYSRRADGRAVLRSSIREYLCSEYMYALSIPTTRALALAVSPDLVYR
                     EQAETAAVVTRAAPSFLRFGHFEHWYHRGLHTDIQLLADLLLEQHYPECLQADNTYLA
                     MFATISQR"
BASE COUNT          859 a          934 c          790 g          730 t
ORIGIN      
        1 cgggcaaagg tggcgtgggc aaatccacta ccaccaccaa tttggccatc gccttgagca
       61 aaatgggcgc gcgcgtgggt gtgcttgatg ccgacattta cggccccagc caacccacca
      121 tgcttggtgt ggcgcatttg aaacccgaac aacgcaatgg caagttcatt cccgtggtgt
      181 ctgacggcgg cattcaaacc atgtcgatag gcttcatgat agacaccgac caagccgtgg
      241 tgtggcgtgg ccccatggtc agccaagctt tgcaacaatt gctgttccaa agccaatggg
      301 acgacgtgga ttatttgttc atcgatttgc caccaggcac aggcgacatt caattgacgc
      361 tgtcacaaaa aatccccgtg accgcatcgg taatcgtcac cacgccacaa gacatcgcct
      421 tgctggacgc gcgcaaagcc gtggacatgt ttgccaaggt caacattccg attttgggca
      481 ttgttgaaaa catggccatg cacgtttgct ccaactgcgg ccatgccgaa gcgatatttg
      541 gcagcgatgg cggcaaggct ttggccgaca aattgaacgt gcctttgttg gcgcaactgc
      601 ctttgagctt gcccatccgc gaggccatgg acgcaggcca aggtgcagcc ttacacagca
      661 aagaaaccgc cattgccgac ttgtaccaac aagccgcttg gcaagtggcc ttaaaagtgg
      721 cagacatggg tcgcgatttc agcggtcgct tcccgaaaat cgtggtggaa taaccccccg
      781 tcttgcatca acatgcggcc tgtaaaacat ttacaggccg catttgcgtg cgcgatggca
      841 ttcatctgcc tgtttgtaca ccacaaatca cacttaaacc tgacgccatc gccaaaatct
      901 tatatcctgt gcgcactatt tttaggacag cagcaatggc actcaaagca accatttaca
      961 aagccaacat caatgtcgcc aacatggatg agcatgtgta cggcgatttt tccctgacca
     1021 tggcgcgtca cccttcggaa accgaagagc gtttgatggt gcgcttattg gcgtattgcc
     1081 gctttgccac gcccgatacc gaaggcttgg aattcaccaa agacttgttt gaaacaggcg
     1141 aacctgcgtt gtggcaacac gatttaaccg gcatgttgac gcagtggata gaagtgggct
     1201 tgcccgatga agacaaggta agaaaagcca gcgcccgctg cgaacgcgtc gctgtggtgg
     1261 cttatggcag caccgcgcca gattggtatc aaaaaagcag caagctcaaa accttgggca
     1321 atgtcagcgt ttggaccttg tctactgcct ctaccgcagc cgtacaagcc ttgtgcagcc
     1381 gtggcatgga cttgcaattg aacatcatgg atggcgaatg gacactcatc agcgacgatg
     1441 cgcaagccat cattgaatgg gaacaatggc aataacgagg attgaaacca taaaaagcgg
     1501 cctgtaaacg atttacaggc cgctttttat aatgaagctg agtattgctg cattcagata
     1561 gaccatattg aacccagcaa accgaaagcg aaaaccgata acacacaggc cgccaagcgt
     1621 tgcaaatact caggccgcaa ccacgtttgc ttggcttgac gcatgctcgc tcccaatgca
     1681 atccaagcac aagcagccat gccaaccaac agcccgaatt ccgccatgat ggtgaaatag
     1741 ccttgccaag agcgccaagt ttccatagga aacaccgccg tggcaaacaa caaggctttg
     1801 ggattgagca aggttgcaag aaacagattc ggggcagcaa tgcggcctgt agattcagaa
     1861 tcagaggctt gagcatgttg ccacaagcgc atggccaaaa aagccaaata cacggcactg
     1921 agggttttca tgatggcagg caattgcggc aagtgagccg tcatttgcgc catgccataa
     1981 ccccacacac tgatggccaa cacatagcca acaagctcaa acggtatcag cctggttgcc
     2041 tgccaccatc cacgttgcaa gcccgctgtt gccaataagg tattggttgg ccctggcgtg
     2101 aacaaaatgc cacccaccaa acccacaaac caccatgaaa ccattgtgtt gtttccaatt
     2161 tgtttgaatt gtctgaggca aaccatacca tggttcggtt cggccaaatt tgcgtcatgg
     2221 taaaccattc tcacccatcc taacgttaat tctgggttaa ttttgtggat ggcaacaggg
     2281 ttttgccttg tttcaacctt gttaaatcca ttccttgccc ataaaaaaag agccgccaat
     2341 catggcggcc aaaataaggg aataatgcac ttctaggact ggcacattcg ctccaagttc
     2401 aggccgattg gcgaccactc ctaccgttta aatctgactt ttcaagcgct taaataccaa
     2461 gcacaccaac aaaccaaaac ccacttaccc tgctgcaatg cttactccca ctttatgcaa
     2521 ggccacccat ttcaacaagc attatggctt tgaattcaca cattccaccc ccatatgtgt
     2581 accatcggtt tgcacctcac ttttgggcaa catcaccata aagacctccc aacatgaaca
     2641 cacagccatc gcatttactc agcctacccg aacaccatta cagcactgtt gcggtgcagc
     2701 ccctgcacgc caccaacctg ttggcctata acgaagcctt agcagcagag ctgggtttgc
     2761 ctgctgctgc cgcgctcaat gaagacgagc gcttgagttt ggcaggcagc ttggaaaacc
     2821 ctccacaagc accgattgcc accgtgtaca gcggccacca attcggcata tgggcagggc
     2881 aattgggcga tggccgcgcc atgttattgg gcgacatcac cgacccgaat gggcaaacat
     2941 gggaattgca actcaagggc gcaggcatca ccccttattc gcgccgcgcc gacggccgcg
     3001 cagtattgcg ctcatccata cgcgagtatt tgtgctctga atacatgtat gctttgagca
     3061 ttcccaccac tcgtgccttg gccttggccg tatcgcccga cttggtttac cgcgagcagg
     3121 ccgaaaccgc cgccgtggtg acacgggcag cgccctcgtt tttgcgcttt ggtcattttg
     3181 aacactggta tcaccgtggt ttacacactg atatacagct attggccgat ttgctgttgg
     3241 aacaacacta cccagagtgt ttacaggccg acaatactta cttggccatg tttgccacta
     3301 tcagccaacg cag
//
LOCUS       DAOMVK010000241         3311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_8447_length_3311_cov_2.240172, whole genome shotgun sequence.
ACCESSION   DAOMVK010000241 DAOMVK010000000
VERSION     DAOMVK010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8447_length_3311_cov_2.240172"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="PK856_08155"
     CDS             complement(<1..618)
                     /locus_tag="PK856_08155"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRL02377.1"
                     /translation="MSPNLHPDLARVTERIRTRSARSRQQYLDMLASMRLQGKRERSQ
                     LSCTNLAHATAAMPNAIKIELHQASAANLAIVSAYNDILSAHQPLKDYPDLIKQAAMS
                     VGATAQFAGGTPAMCDGVTQGQAGMELSLFSRDVIAMSTAIALSHQMFDATVCLGVCD
                     KIVPGLLIGALSFGHLPTVFVPAGPMETGISNDEKAKIRQLYATGQ"
     gene            783..2255
                     /gene="zwf"
                     /locus_tag="PK856_08160"
     CDS             783..2255
                     /gene="zwf"
                     /locus_tag="PK856_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HRL02378.1"
                     /translation="MSKTLQHSKPPFDMVLFGGTGDLAMRKLLPALYAAHLQGLLHVT
                     GRILGLGRQDLGRAGYLAQLEAEARPRIKAWDAQAWEGFLARIDYLQLEATESADYAR
                     LAAALNSATPKLNVYYLATAPKLFEPICAHLAEVGLQAEPHRVVLEKPLGTDLASSNA
                     INEAIARYFHESQIYRIDHYLGKESVQNLLALRFANVLFEPLWRREWVEHVQITIAEK
                     LGVAGRGEFYDGIGALRDMVQNHLLQLLCFVAMEPPNSLQADAVRNEKIKVLQSLKPL
                     RSSDVPKQVVRGQYQAGEIDAVPVPGYVAEEDTPEHSNTETFVALKAKIDNWRWAGVP
                     FFLRTGKRMPEKVAEIVIQFREVPHAVFPSVGGMSAGNRLVIRLQPEEDIRLYLMAKE
                     PGNAMKVKPTYLSLDFCQVFAGRRAEAYERLLLDVIDGKLALFMRRDEVMAAWEWVTP
                     IMRYWAEEETGPLPYLAGTWGPEAATVLLSKIGVAWHDDE"
     gene            2242..2955
                     /gene="pgl"
                     /locus_tag="PK856_08165"
     CDS             2242..2955
                     /gene="pgl"
                     /locus_tag="PK856_08165"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HRL02379.1"
                     /translation="MTTNETWHCFSDANACSLALAQALTQELQRLLSLQARVVLAVSG
                     GQSPRAMMAALSHAGVDWSRVLIQWVDERLLSRHHPNSNSELIESVLRQHQAAVSQWH
                     NELPEVTDSPRELTDVEQQAVLQAALSFYKKPDIVVLGMGEDGHTASLFPRAKQLPQA
                     LNLHNTVPLMLLQDVVAPYARISMTLPAILSAKVIFIAAYGQQKREVLQQAAKQLDAN
                     LPISSVFHHAQKIPVYVYC"
     assembly_gap    2984..2993
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2994..>3311
                     /locus_tag="PK856_08170"
     CDS             2994..>3311
                     /locus_tag="PK856_08170"
                     /inference="COORDINATES: protein motif:HMM:NF014715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HRL02380.1"
                     /translation="MTTAPFSLVADIGGTHARFALEVQPLELHHVMVLASENFASMEA
                     AINHYVTSVGQPRIQHAAFAMANPVLGDVVQMTNHHWTFSIASMRLAMGWETLLVVND
                     FSAQ"
BASE COUNT          738 a          785 c          954 g          824 t
ORIGIN      
        1 ttggccggtg gcatacaact gacgtatctt ggctttttca tcgttgctga tgcctgtttc
       61 catgggccct gcgggcacga atacggtggg caaatgaccg aagctcaaag cacctatcaa
      121 taggcctggc acgattttgt cgcacacgcc caagcacacg gtggcatcga acatttggtg
      181 tgataaggca atggcggtgc tcatggcgat gacatcgcgc gaaaacaaag acaactccat
      241 gcctgcttgg ccttgggtga caccgtcgca catggcaggc gtgccacctg caaactgtgc
      301 cgtcgcgccc acactcatgg cggcttgttt gatcaggtcg ggatagtctt tgagcggctg
      361 gtgcgcggat aaaatgtcgt tataggcaga cacgatggcc agattggctg cagatgcttg
      421 gtgcaattca atcttaatgg cgttgggcat ggcggcggtg gcatgggcca aattggtgca
      481 cgacaattgg ctgcgctcac gcttgccttg caggcgcatg ctcgcaagca tgtccaaata
      541 ctgctggcgg ctgcgcgcgc tgcgcgtgcg tatgcgctca gtcactcgtg ccaaatcggg
      601 atgaaggttg gggctcatgg tgaggttcct gtgcaggtaa gccgaaaaga catgatacgg
      661 cgttttcagt ttggttccca gtggctgtct ggtcttggct ttacaggccg cttgtggctg
      721 gaatctgggc acatgatgca gtaacatgaa tcccatctgc atgcacagca ttggaggtaa
      781 ccatgtctaa aaccttacaa cacagcaaac cgccgttcga tatggttttg ttcggtggaa
      841 cgggcgattt ggccatgcgc aagctgttgc ctgctttgta tgccgcccat ttgcaaggct
      901 tattgcatgt tacaggccgc atcttgggct tgggccgcca agacttgggg cgggcaggtt
      961 atttggcgca attggaagcg gaggccagac cccgtatcaa ggcttgggat gcgcaagcat
     1021 gggaaggttt tttggcccgc attgattacc tgcaactaga ggccaccgag agtgcagact
     1081 atgcacgctt ggctgctgcg ctgaacagcg ccactcccaa attaaacgta tattacttgg
     1141 ccactgcacc caaattgttt gagccaattt gtgcccactt ggccgaggtc ggcctgcaag
     1201 cagaaccgca tagggtggtg ttggaaaagc ctttgggcac cgatttggcc tcgtctaatg
     1261 ccatcaacga agcgattgcg cggtattttc atgagtcaca aatttaccgc atagaccact
     1321 atttgggcaa agagtcggtg caaaatctgc tcgccctgcg ttttgccaat gtcttgtttg
     1381 agcccttgtg gcggcgtgaa tgggtggagc atgtgcaaat caccatagca gagaaactgg
     1441 gcgtggcggg tcgtggtgag ttttacgatg gaataggtgc tttgcgcgac atggtgcaaa
     1501 accatttgct gcagttactg tgttttgtgg cgatggagcc gccgaacagt ttacaggccg
     1561 atgcggtgcg caatgaaaaa attaaggtgt tgcaatcctt gaaaccgctg cgcagcagtg
     1621 atgtgcctaa acaagtggtg cgcgggcaat accaagcagg cgagattgat gccgtgcctg
     1681 tgcctggcta tgtggcagaa gaagacacac ccgagcacag caataccgaa acttttgtcg
     1741 ccttgaaagc caaaattgac aattggcgtt gggctggggt gcctttcttt ttgcgcacag
     1801 gcaaacgtat gccagagaaa gtggccgaaa ttgtgattca atttcgagaa gtgccgcatg
     1861 cggtgtttcc aagtgttggg ggaatgagcg cgggcaaccg tttggtcatc cgtttgcaac
     1921 ctgaggaaga catccgtttg tatttgatgg ccaaagagcc tggcaatgcg atgaaggtga
     1981 agcccacgta tttgagtttg gatttttgcc aagtatttgc ggggcgcaga gccgaagcct
     2041 acgagcgctt attgctggac gtgatagacg gtaaattggc tttgttcatg cgccgcgatg
     2101 aggtgatggc ggcatgggaa tgggtaacac ccatcatgcg ctattgggcg gaagaagaga
     2161 ctgggccatt gccttacttg gcaggaactt gggggcctga ggccgctacg gtgttgttgt
     2221 caaaaattgg ggtggcttgg catgacgacg aatgaaacat ggcattgctt ttccgatgca
     2281 aacgcttgca gcttggcttt ggctcaagcg ctgacccaag agctgcaaag attgctcagt
     2341 ttgcaagcgc gcgtggtatt ggcggtttca ggtgggcaat caccgcgggc gatgatggct
     2401 gctttaagcc atgccggtgt ggattggtcg cgcgtgttga ttcaatgggt agacgagcgc
     2461 ttgctctctc gccaccatcc aaacagcaac agtgagctga tagaaagcgt attaagacaa
     2521 caccaagctg ccgtatctca atggcacaat gaattgcctg aggtgacaga ttcgcctcgt
     2581 gaattgacag atgttgagca gcaagcggtg ttacaggccg cattaagctt ttacaaaaaa
     2641 cctgatattg tggtgttggg catgggagaa gatggccata ctgcatcatt gtttccccgt
     2701 gccaagcaat tgcctcaggc gctgaatttg cacaacacag tgcctttgat gctgttgcaa
     2761 gatgtggttg cgccttatgc ccgcatcagc atgacactgc ctgcaatttt atctgccaaa
     2821 gtcattttta tcgccgccta tggtcagcaa aaacgggagg tattgcaaca agccgccaaa
     2881 caactggatg ccaacttgcc catcagcagc gtgtttcatc atgcacagaa aattccagtc
     2941 tatgtctatt gctaagacct cattcccttc ttcaatcaat tttnnnnnnn nnnatgacaa
     3001 ccgccccctt ttcgctggtg gctgatattg gtggcaccca cgctcgcttt gcattggaag
     3061 tgcagcctct tgagttgcac catgtcatgg tgttggccag tgagaatttt gcgagcatgg
     3121 aggctgccat aaaccattat gtaacttcgg tggggcagcc acgtatccag catgctgcct
     3181 ttgccatggc caatcctgtt ttgggcgatg tcgtgcagat gaccaatcac cattggactt
     3241 tttccattgc ctccatgcgc ttggccatgg gttgggaaac tctgctcgtg gtgaatgatt
     3301 tcagtgcgca g
//
LOCUS       DAOMVK010000242         3308 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_8461_length_3308_cov_2.672302, whole genome shotgun sequence.
ACCESSION   DAOMVK010000242 DAOMVK010000000
VERSION     DAOMVK010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8461_length_3308_cov_2.672302"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="PK856_08175"
     CDS             complement(<1..689)
                     /locus_tag="PK856_08175"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRL02381.1"
                     /translation="MADGYARASGKVGVCFIITGPGMSNIATAMAQAKADSIPMLVIS
                     SVNKIAHTGSGAGHLHELDNQQALVEQVAAYSRTVWSVDTLPEILAEAFNLFDSAKPA
                     PVHIQLPLDIITADASHVQWHISKSHRPAASAQALDEAAACLQAASAPMLLLGGGCVD
                     VPPEHIRALAECLDAPTTLSINAKGLLPIGHPLNLGSNATFAPVRKLIAEADVVLAIG
                     TELGQTDYDLYV"
     gene            complement(620..853)
                     /locus_tag="PK856_08180"
     CDS             complement(620..853)
                     /locus_tag="PK856_08180"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRL02382.1"
                     /translation="MNKKTCGELLVEILAAYGVDTVFGIPGVHTVELYRGLPNTPIRH
                     ITPRTNKAQALWPMAMPALQARWACVSSSLALA"
     gene            complement(883..2502)
                     /locus_tag="PK856_08185"
     CDS             complement(883..2502)
                     /locus_tag="PK856_08185"
                     /inference="COORDINATES: protein motif:HMM:NF007412.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HRL02383.1"
                     /translation="MKNTATVDKPLAILTLAIVFASVAGLAIYSTESIALAATMMGWV
                     TRVFTTPVLIFTLLSVVFCLWLAFSKYGHIKFGHEKPEYSTMTWIFMFFLSGMGSATL
                     YWGFLDWAYYYQTPGLNIPAQTPEAFKYSVAYSFFHYGPSAWSIFALTSVPLCYHYYV
                     RKNKGLSLAAIIEAVTGYKATGPVGRLVDLLFLLCMFGALTISLVLTAVTFTNILSLL
                     TGIPNTFTTKVTIILTVSVLFALSSYVGMDSGMKRLSQLVCFGIIAFAVYIFIFGPTE
                     FILNNSLSSLGLMSTRFMDMSLFTDPTGSGQFTRDWTVFYWLWWISYAPGVALFVTRV
                     SRGRSIREVVLALIVAGCAASWSMYGILENFSAHSFLNGLVDVPAILATKGGEVAIGE
                     LLNLLPAGTALMWAFLIIMAVYLAAHMDAVGYAVSATCVRNLKEGQDPSPNSRLFWCI
                     MLTLVPIAMIFSKAPLDTMKAATVITALPFIVIILIQTYGLVKWLKQDYGHVPAHKIE
                     QEYMSVRSSTKTSEHEAQHAVSPANVSADKS"
     gene            complement(2809..>3308)
                     /locus_tag="PK856_08190"
     CDS             complement(2809..>3308)
                     /locus_tag="PK856_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE-like domain-containing protein"
                     /protein_id="HRL02384.1"
                     /translation="HDQEEALEISDQIVVMNHGKVEQVGSGAKLYEEPDNAFVTEFLG
                     EVNRFEGYIERGELVLGTYHYQPLPPQEKWHEGTATAYIRPHEFNISRDSEGAMLQAD
                     IHHISVLGPIVRVSLYDKSGQDIEVMIPHQHHLANQFVVGERVYLTPSNISVFTQKDM
                     VDYVI"
BASE COUNT          873 a          822 c          869 g          744 t
ORIGIN      
        1 acatacaaat cgtaatcggt ttgtcccaat tccgtgccga ttgccaacac cacatcggcc
       61 tcggcaatca gtttgcgcac cggtgcaaac gtggcattgc tgcccaagtt aagcggatgg
      121 cctatcggca acaagccttt ggcattgatg ctgagggtgg tgggcgcatc caaacattct
      181 gccaaagcac ggatgtgctc aggtggcaca tcgacacaac cgccaccgag caagagcatg
      241 ggcgcagatg cggcctgtaa acacgctgcg gcctcgtcta aagcttgcgc tgaggcagca
      301 ggtctgtggc ttttgctgat gtgccactgc acgtggctgg cgtcggcagt gatgatgtcg
      361 agcggcaatt gaatgtgtac gggcgcgggt ttggcactgt cgaacaggtt aaacgcttcg
      421 gccaaaatct caggcagcgt gtccacgctc caaaccgtgc ggctgtacgc cgccacttgt
      481 tcaaccaagg cttgttggtt gtccaactcg tgcaagtggc cagcaccgct gccggtgtgg
      541 gcgattttgt tgacactgga aatcaccaac atcgggatgg aatcggcttt ggcttgcgcc
      601 attgccgtgg cgatgttgct catgccaggg ccagtgatga tgaaacacac gcccaccttg
      661 cctgaagcgc gggcatagcc atcggccata aagcctgcgc cttgttcgtg cgcggggtga
      721 tgtgacggat gggggtgttg ggtaggccgc ggtacaactc cacggtgtgc acgccaggaa
      781 tgccaaacac cgtgtccacg ccgtaagccg ccaagatttc aaccaataat tcaccacagg
      841 tttttttatt catgctcatc ctttcttgtt ttgttctatg gcttaagatt tgtcggcgct
      901 gacattggcc gggctgacag catgttgtgc ctcatgctca gaggtttttg tagatgagcg
      961 taccgacata tactcttgct caattttatg cgcaggcacg tggccgtagt cttgtttcag
     1021 ccatttcacc aagccataag tctgtatcaa aatgatgacg atgaagggca gcgcggtaat
     1081 cacggtggcg gccttcatgg tgtccagcgg cgctttggag aaaatcatgg cgatgggtac
     1141 caaggtcaac atgatgcacc aaaacaagcg tgaattaggt gaagggtctt ggccttcttt
     1201 caagttgcgt acacaagtgg ccgaaaccgc atagcctacc gcatccatgt gtgccgccaa
     1261 atacacggcc atgatgatga gaaaggccca catcaaggct gtacccgcag gcagtaaatt
     1321 caacaattca cctatggcca cttcgccacc tttggtggcc aagatggcag gcacatcaac
     1381 caaaccgttt aagaaactgt gggcgctgaa gttttccaaa atgccataca tagaccaact
     1441 ggcggcacaa ccggccacaa tcaaagccaa aaccacttcg cgtatgctgc gaccgcgtga
     1501 cacacgggtc acaaacaaag ccacacctgg cgcgtacgaa atccaccaca accaataaaa
     1561 caccgtccaa tcacgggtga attggccact gcctgttggg tcggtgaaca aactcatgtc
     1621 catgaaacgg gtagacatca ggcccaagct gcttaaagag ttgttgagga tgaattcggt
     1681 ggggccaaaa ataaagatgt aaaccgcaaa agcgatgatg ccaaaacaca ccaactgact
     1741 caaacgcttc atgccgctgt ccatgcccac gtaagagctc aaggcaaaca acacggacac
     1801 ggtcaaaatg atggtgactt tggtggtaaa ggtattgggt atgccggtga gcaaagacaa
     1861 aatattcgtg aaggtgaccg cggtcaacac caaagaaatg gtcagcgcac caaacatgca
     1921 cagtaaaaac agcaaatcca ccaagcggcc cactgggcct gtggctttgt agcctgtgac
     1981 ggcttcaatg atggcggcca aactcaagcc tttgtttttg cgcacataat agtgataaca
     2041 caaaggcacc gaagttagcg caaaaataga ccaagcgctg gggccgtaat gaaagaaaga
     2101 ataggccacg ctgtatttga aggcctcagg ggtttgggcg gggatgttca ggccgggtgt
     2161 ttggtagtaa tacgcccaat ccaaaaagcc ccaatacaaa gtggccgaac ccataccaga
     2221 caggaaaaac atgaagatcc acgtcatggt ggagtattcg ggtttttcat ggccaaactt
     2281 gatgtggccg tatttgctga aggccaacca caggcagaaa acgacggaca ataaggtgaa
     2341 aatcaagacg ggtgtggtga atacgcgggt gacccatccc atcatggtgg cggccagcgc
     2401 aatcgactcg gttgagtaaa tcgccagacc tgccacggaa gcaaagacga tggccaaagt
     2461 cagtatggcc aatggtttgt ccacggtagc tgtgtttttc ataattctcc tagtgtgcat
     2521 gtgggcttgt tataacaagc ctcttcaaat ggtatcggta caaagaaaca gacaaatact
     2581 cgctttgaac ataacataaa gcctgttgaa cacatgacag cagagcgtga acagtatcat
     2641 cacgaatcat gaacagtgaa aaatagacag tgttgtcaat gctttgttgg gcatggaaac
     2701 gcatgatgac aatgcccttg caaacatggc ataccaattt gagcgggacc agaaatgttt
     2761 acaggccgca taagcggcct gtaaacatat tttaagcatt aaaaaacatc aaatcacata
     2821 atccaccatg tctttttggg taaagacgct gatgttactc ggggtgaggt agacacgttc
     2881 gcccaccaca aattggttgg ccaaatggtg ttggtgcgga atcatcactt caatgtcttg
     2941 acctgatttg tcgtacaaag acacgcgcac gatggggcct aacaccgaaa tgtggtggat
     3001 gtctgcctgt aacattgcgc cttcactgtc gcggctgatg ttgaattcat gcgggcggat
     3061 ataggcggtg gccgtgcctt cgtgccattt ttcttgcggt ggcaagggtt ggtagtggta
     3121 agtacccaat accaattcgc cacgctcgat atagccttca aagcggttca cttcgcccaa
     3181 aaattcggtg acaaacgcat tgtcaggttc ttcgtacaat ttcgcgccgc tgcccacttg
     3241 ttcgactttg ccgtggttca tcaccacgat ttggtcggaa atttccaagg cttcttcttg
     3301 gtcgtggg
//
LOCUS       DAOMVK010000243         3284 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_8545_length_3284_cov_3.512233, whole genome shotgun sequence.
ACCESSION   DAOMVK010000243 DAOMVK010000000
VERSION     DAOMVK010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8545_length_3284_cov_3.512233"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1279)
                     /gene="benA"
                     /locus_tag="PK856_08195"
     CDS             complement(<1..1279)
                     /gene="benA"
                     /locus_tag="PK856_08195"
                     /EC_number="1.14.12.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004925496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate 1,2-dioxygenase large subunit"
                     /protein_id="HRL02385.1"
                     /translation="MPTIPIISVSHLDHIDDLLVENFESGEFKLHRSVFTDQTLFDLE
                     MKYIFEGNWVFLAHESQIPNNNDYYTTHIGRQPVLIARNKNGELNAMINACSHRGAQL
                     CRYKRGNKATYTCPFHGWTFNNSGKLLKVKDPGEAGYSDCFNHEGSHDLKKVARFENY
                     KGFLFGSLNADVLPLTEYLGETTKIIDMIVDQSDQGLEVLRGSSSYTYEGNWKLTAEN
                     GADGYHVSAVHWNYAATTQQRKEKQQENDNVRAMSAGSWGKQGGGSYGFDNGHMLLWT
                     QWGNPEDRPNFPKADEYTEKYGAAMSKWMIERSRNLCLYPNVYLMDQFGSQIRVLRPI
                     SVNKTEVTIYCIGPVGEEPAARTRRIRQYEDFFNASGMATPDDLEEFRACQVGYAGIE
                     LEWNDMCRGAKHWIHGPDDAANEIGLKPKLSCIK"
     gene            complement(1359..2279)
                     /gene="catA"
                     /locus_tag="PK856_08200"
     CDS             complement(1359..2279)
                     /gene="catA"
                     /locus_tag="PK856_08200"
                     /EC_number="1.13.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018576 - catechol 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019615 - catechol catabolic process,
                     ortho-cleavage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catechol 1,2-dioxygenase"
                     /protein_id="HRL02386.1"
                     /translation="MEHAKIDALVNKMNVDTATGPVDARVQQVVIRLLSDLFKAIEDL
                     DITPSEVWKGLEYMTDAGQANELGLLAAGLGLEHFLDLRADEADAKAGIHGGTPRTIE
                     GPLYVAGAPESVGFARMDDGSESDTLDTLYIEGVVKDTEGNVIEGAKVEIWHANGLGT
                     YSFFDKRQSEFNLRRTILADKAGHYLAQTTMPVGYGCPPQGTTQALLNKLGRHGNRPS
                     HVHYFVSAPGYRKLTTQFNIEGDQYLWDDFAFATRDGLVAVATDVTDPAELKRLGATS
                     PIKHITFNVELVKDKAAAPTAEVDRRRAAA"
     gene            complement(2373..2663)
                     /gene="catC"
                     /locus_tag="PK856_08205"
     CDS             complement(2373..2663)
                     /gene="catC"
                     /locus_tag="PK856_08205"
                     /EC_number="5.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007483157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016159 - muconolactone delta-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muconolactone Delta-isomerase"
                     /protein_id="HRL02387.1"
                     /translation="MLFHVRMDVHIPLDMPAEKAAEIKAVEKAYSQNLQQQGKWRHIW
                     RVTGQYSNISIFDVDSNEELHTILQGLPLYPYMDIEVMALNRHPSSVRDDDS"
     gene            complement(2678..>3284)
                     /locus_tag="PK856_08210"
     CDS             complement(2678..>3284)
                     /locus_tag="PK856_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HRL02388.1"
                     /translation="NPWQQDVEHVIAIKQALGSDISVRVDVNRAWSEMDCIKGIQALQ
                     DGGIDLVEQPCAIENTDALRRLTERFDVAIMADEALTGPHSAYRLAKQYGAHVFAVKI
                     AQSGGLLEAKEVATVAKLAGIDLYGGTMLEGPIGSIASAHTFATFDNLAFGTELFGPL
                     LLTEDILQEPLQYRDFELHLPTGPGLGVVVDENKIDALRHQ"
BASE COUNT          776 a          837 c          798 g          873 t
ORIGIN      
        1 ttttgatgca gctgagtttc ggtttcaggc cgatttcatt ggcggcatcg tctggtccat
       61 gtatccagtg tttggcaccg cggcacatgt cgttccattc cagctctatg cctgcatagc
      121 ccacttggca cgcgcgaaac tcttccagat cgtctggcgt ggccatgcct gaggcattga
      181 agaagtcttc gtattggcgg atgcggcggg tacgcgcggc aggctcttca cccacagggc
      241 caatacagta aatggtgact tcggttttgt tgacagaaat cgggcgcaac acgcggattt
      301 gtgaaccaaa ttggtccatc aaatacacat ttgggtacaa acacagattg cgcgagcgtt
      361 ctatcatcca tttcgacatc gccgcaccgt atttttcagt gtattcatcg gctttaggga
      421 agttaggacg gtcttcaggg ttgccccatt gcgtccacaa caacatgtga ccattgtcaa
      481 aaccatatga tccgccgcct tgtttgcccc atgagcctgc actcatggca cgcacattgt
      541 cgttttcttg ctgtttctct ttgcgttgtt gggtggtggc cgcgtaattc caatgcacag
      601 ctgacacgtg gtagccatcg gcaccatttt ctgccgtgag tttccagttg ccttcatacg
      661 tataagaaga tgagccgcgc aacacttcca agccttgatc ggattggtca acaatcatgt
      721 cgatgatttt ggtggtctca cccagatact cagtcaaagg caagacatcg gcattcaaac
      781 taccaaacaa aaagcctttg tagttttcaa agcgggccac ttttttcaag tcgtgcgagc
      841 cttcgtgatt gaaacaatct gaataaccag cttcacctgg gtctttgact ttcagcaatt
      901 tgcctgaatt gttaaacgtc cagccgtgga acgggcaggt gtaagtggct ttgttaccgc
      961 gcttgtaacg acacaactgc gcaccacggt gagagcaggc attgatcatg gcattgagct
     1021 caccgttttt attgcgggca atcaacaccg gttggcgacc aatgtgggtg gtgtaatagt
     1081 cgttgttatt ggggatttgg ctttcgtggg ccaagaaaac ccaattgcct tcaaaaatgt
     1141 atttcatttc caaatcaaac aaggtttgat cggtaaacac agagcggtgt aatttgaatt
     1201 cgccgctttc aaaattttca accaataagt cgtcgatgtg atccaagtgg ctgacactga
     1261 taatcggaat ggtaggcatg gtgtgctcca atcaaactga gtttattttt ctctaagcgg
     1321 cgttacaggc cgcttagaat gctttggaaa ggctcgtatt aagcagcagc gcggcgacgg
     1381 tcaacttcgg cagtcggtgc tgcggctttg tctttcacca gctccacatt gaaggtaatg
     1441 tgtttgattg gactggttgc acccaagcgt ttcaactcag cagggtcggt cacgtctgtt
     1501 gcgactgcca ccaagccatc gcgggtcgca aaggcaaagt catcccacag gtattgatcg
     1561 ccttcaatgt tgaattgggt ggtgagtttg cggtaaccag gggcagacac aaagtaatgc
     1621 acgtgtgatg gacggttgcc atggcgaccc aatttgttga gcaaggcttg ggttgtgcct
     1681 tgtggcggac aaccatagcc cacaggcatg gtggtttgtg ccaagtaatg gcctgccttg
     1741 tcggcaagaa tggtgcgacg caagttgaat tcagattggc gcttgtcaaa gaaagaatac
     1801 gtacccaaac cattggcgtg ccaaatttcg acttttgcgc cttcaatgac gttgccttcg
     1861 gtgtctttca ccacgccttc gatgtacaaa gtgtctaagg tgtctgattc agaaccatcg
     1921 tccatgcgcg caaagcccac gctttcaggc gcaccagcca catacaacgg gccttcaatc
     1981 gtgcgtggtg taccgccgtg tatgcctgct ttggcatcgg cttcgtctgc gcgcaaatcc
     2041 aagaaatgct ccaagcccaa acctgcggcc agcaagccca attcattggc ttgacctgca
     2101 tcggtcatgt actccaagcc tttccacact tcgcttgggg taatgtccaa atcttcaatg
     2161 gctttgaata aatcacttaa caaacgtatc accacttgtt gcacacgggc atccactggg
     2221 ccagtggcgg tgtccacgtt cattttgtta accaacgcat cgatttttgc atgttccatg
     2281 ataaaactcc tttgtaatca ctgattgttt atgaatgact gcttaagatg tggtcgcgct
     2341 tctgcgagtg cgtaccccac ccgatactgc gattaagaat cgtcgtcacg caccgatgat
     2401 gggtgacggt tcaaagccat cacttcgata tccatgtaag gatacaaagg taagccttgc
     2461 aagatggtgt gtaattcttc gttgctatcc acatcgaaaa tgctgatgtt ggaatattgg
     2521 ccagtcacgc gccaaatgtg acgccacttg ccttgttgtt gtaagttttg agaataggct
     2581 ttctctacgg ctttgatttc tgccgctttt tcagctggca tatccagagg gatatgcaca
     2641 tccatgcgca cgtgaaacag catgtcgtct cctttgttta ttgatgacgc aaagcgtcga
     2701 ttttgttttc atccaccacc acacccaaac cagggccagt gggcaggtgt aattcaaaat
     2761 cacggtattg caagggctct tgcaaaatgt cttcggtgag caacaatggc ccaaacaact
     2821 ccgtaccaaa cgccaaatta tcaaaggtag cgaaggtgtg ggccgaagca atcgaaccaa
     2881 ttgggccttc caacatggtg ccaccgtaca aatcaatgcc tgccagcttg gcaaccgtgg
     2941 ccacttcttt ggcttccaac aagccgcccg attgggcaat tttcaccgca aacacatgcg
     3001 cgccatattg cttggcaaga cggtaagcac tgtgcggccc agttaaagct tcatctgcca
     3061 tgatcgccac atcaaaacgc tcggttaaac ggcgcagcgc atcggtgttc tcaatcgcac
     3121 aaggctgttc aaccaaatca atgccgccgt cttgcaaggc ttgtatgcct ttgatgcaat
     3181 ccatttccga ccaagctcgg ttcacatcca cgcgcacgct gatgtcgctg cccaaggctt
     3241 gcttgatggc aatcacatgt tccacatctt gctgccatgg attg
//
LOCUS       DAOMVK010000244         3281 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_8558_length_3281_cov_2.789523, whole genome shotgun sequence.
ACCESSION   DAOMVK010000244 DAOMVK010000000
VERSION     DAOMVK010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8558_length_3281_cov_2.789523"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="PK856_08215"
     CDS             <1..703
                     /locus_tag="PK856_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02389.1"
                     /translation="TNASAQAEAADQAYSNTPRSQSAKGSERLGTQWGDDVDSHVTSV
                     DLRRVSEAPIAQTSVRYADKRYSGRAVNSISLAAGKIEFSVESDRGRNLSLYRDGSQY
                     YLKGEAGQAYRLVYRNNSNNTYEIVSSVDGLDVLNGSRASRYNTGYVLRPKERLVIEG
                     FRKSNSAVASFIFSKPDDSYAANTPNGNVNNTGIIGTAVYELYDPQARKRPVSRSSDG
                     LDAFPADEGYAPAPR"
     gene            906..1373
                     /locus_tag="PK856_08220"
     CDS             906..1373
                     /locus_tag="PK856_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009116016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRL02390.1"
                     /translation="MNIAPYLNFDGTCAEAMAFYAQVFQGTIVHQSTFGEMPPDPNMP
                     PLPESAMGRIMHAHLQFGHNNIMASDSFPSIPGMDEQACVGGYQKPQGLWVSIGVDSA
                     AEGQRIFDALAEGGTVAMPYAATFWSEGFGMVTDRFATPWMVNVSDSTNTELS"
     gene            complement(2005..2742)
                     /gene="ubiE"
                     /locus_tag="PK856_08225"
     CDS             complement(2005..2742)
                     /gene="ubiE"
                     /locus_tag="PK856_08225"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HRL02391.1"
                     /translation="MTDKQTHFGYKTVDEAEKAGKVAEVFHSVAGKYDIMNDVMSGGL
                     HRVWKHFTINTARLHKGEKVLDIAGGTGDLSRGWKKRVGDKGEVWLTDINSSMLTVGR
                     DRLLNEGTVTPVALCDAEKLPFPDNYFNLVSVAFGLRNMTHKDQALKEMYRVLKPGGQ
                     LLVLEFSKVAKPLAPVYDFYSFKFLPTFGKVIAKDADSYQYLAESIRMHPDQETLQQM
                     MYDAGFDLVDYHNLTAGVVALHKGTKL"
     gene            complement(2799..3152)
                     /locus_tag="PK856_08230"
     CDS             complement(2799..3152)
                     /locus_tag="PK856_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HRL02392.1"
                     /translation="MTAIPSAIRLDKSKTVLLLDYEQEQYSLSAEYLRVYSPSAEVRG
                     HGVGQEVLQVGKRAVRIVGVEAVGNYALRIVFSDGHDSGLYDWAYLHELALSEPEKWS
                     QYLQRLTAAGASRDA"
     gene            complement(3155..>3281)
                     /locus_tag="PK856_08235"
     CDS             complement(3155..>3281)
                     /locus_tag="PK856_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HRL02393.1"
                     /translation="YEAIMALYQHAIDHEYRFFSYGDAMVLTRQDTQGLTPFIEA"
BASE COUNT          892 a          841 c          707 g          841 t
ORIGIN      
        1 taccaatgcc agtgcccagg ccgaagcagc agaccaagcc tactccaata cgccacgctc
       61 tcaaagtgcg aaaggctcag aacgcttggg cacgcaatgg ggtgacgatg tggattctca
      121 cgtgaccagt gtggatttgc gccgtgtgtc tgaagcgccc atcgctcaaa ccagtgtgcg
      181 ctatgccgac aaacgctatt caggccgcgc ggtgaacagc atttctttgg ctgccggcaa
      241 aatcgaattc agcgtggagt ccgaccgtgg ccgcaatctg agcctgtacc gtgatggcag
      301 ccaatactat ttaaaaggcg aagccggcca agcctatcgc ttggtatacc gcaacaacag
      361 caacaacacc tatgaaattg tgtccagcgt ggatggctta gacgtactca atggcagccg
      421 tgccagccgc tacaatactg gttatgtgct gcgcccaaaa gagcgtttgg tcatcgaggg
      481 cttccgcaaa agcaacagcg ccgttgcctc attcatcttc agcaagcctg acgactcgta
      541 tgcggccaac actccaaatg gcaatgtcaa taacacaggc atcataggta cggcggttta
      601 tgaactctac gacccgcaag cacgcaagcg gcctgtaagc cgcagcagtg atggtttgga
      661 tgcctttcct gccgatgaag gctacgcccc agctccacgc tgatgctttg tatgcatagc
      721 aaaaagatat ctgcctttac tggcagatat cttttttttg ttttggttac agctgaaaat
      781 acagtaagct gttgttcctg tttcaccctt tgcacaatca gcatgcggcg tttcaacaat
      841 acaggccgca tgttaattga tgacttggga cagattttct caacaaacac gtagagagga
      901 tacacatgaa tattgcacct tatctgaatt tcgacggcac ttgtgccgaa gccatggctt
      961 tttacgctca ggtctttcaa ggcaccatcg tgcaccaatc cacgtttggc gaaatgccac
     1021 cagaccccaa tatgccgccg ctgcctgagt cagccatggg ccgcatcatg cacgcccatt
     1081 tgcagtttgg gcacaataac atcatggcct cagattcttt tccaagcatt cctggtatgg
     1141 atgaacaagc ctgtgtaggc ggctaccaaa agccacaagg gctgtgggtg tccataggtg
     1201 tggacagcgc cgcagaaggc caacggatat ttgatgcctt ggctgaaggt ggcaccgtcg
     1261 ccatgcctta tgccgcaact ttttggtcag aaggcttcgg catggtcacc gaccgctttg
     1321 ccaccccttg gatggttaat gtaagcgact caactaatac agaactttcc taaaaacact
     1381 tacaacacaa cccaagcaac ttagcactca tgaggtagct aaaacagtaa tgacttcaat
     1441 ccacaaatga tattatgcat tttgagtttt aaaactcatt ttagattatt acatttagat
     1501 ttagtctact ttccaaacca aacaacgaaa aatggtttta ctagtaatac ttcttctact
     1561 taatttttga agaaacaact ccagaatagg aattttatgc ataaagtaat agccatttca
     1621 ttgattctac tcacctcaac ttcgtcattt gcatggacga actcaataca catcggaaat
     1681 aacactagca catatgattc cgacaatggc ttctctagtt ctaaccggat aggtaacaat
     1741 actagtacat acaatttgaa ctcaactcaa gacactaatg attacaacca acaaagagaa
     1801 tcaaaccgcc cctctatagg attaccctca actcaaattg gtaatgggac gtacaattca
     1861 aatggcactt catgtcaaag aattggaaat atcatgtcat gtatttaatt catctcaata
     1921 ttaaatacca ttccatacaa tagtacttta aaccaacaaa aagccctcgt gaaacacaag
     1981 ggcttttgaa tggatatgta tggcttacag tttggtgcct ttgtgcaaag ccaccacgcc
     2041 agcggtcagg ttgtgataat cgaccaaatc aaaacctgcg tcatacatca tttgttgcaa
     2101 ggtttcttgg tctgggtgca tgcgtatcga ctcagccaaa tactgataag aatcggcatc
     2161 tttggcgatg actttgccga aagtcggcaa gaatttgaac gagtaaaaat cgtaaacagg
     2221 cgccaaaggc ttggccactt tggaaaattc caataccaac aattggccac ctggtttgag
     2281 cacgcggtac atttctttca aggcttggtc tttgtgggtc atgtttctga gaccaaatgc
     2341 caccgatacc aaattgaaat aattgtctgg gaacggcagt ttttcagcat cacacaaagc
     2401 cacaggcgtg accgtgcctt cattgagcaa gcggtcgcgg ccaacggtta acatagacga
     2461 attgatgtcg gttaaccaca cttcgccttt gtcaccgacg cgctttttcc aaccgcgcga
     2521 caaatcgcct gtgccgcctg cgatgtccaa tactttttca cctttgtgca aacgtgcggt
     2581 attgatggtg aaatgcttcc acacacggtg caggccgccc gacatcacgt cgttcatgat
     2641 gtcgtatttc cccgcaaccg aatgaaatac ctcagccact ttaccggctt tttctgcttc
     2701 gtctacggtt ttgtatccga aatgggtttg cttgtctgtc attgttttgg ttcttccaaa
     2761 tgcggcctga aagcctgatt gtaacctact cagaggcttt atgcgtcacg agatgcacca
     2821 gctgcagtca atctttgcaa atattgtgac catttttctg gctcactcaa agccaattca
     2881 tgcaaataag cccaatcgta taagcccgaa tcatggccat cgctgaacac gattctcaac
     2941 gcataattgc ccaccgcttc cacacccaca atgcgcacgg cgcgtttgcc cacttgcaac
     3001 acctcttgac ccacgccatg accacgcact tcggcactgg gcgagtacac acgcagatac
     3061 tcggcgctga gggaatactg ctcttgctca taatccaaaa gcaacacagt tttggatttg
     3121 tccaaacgaa tggcgctggg tatcgctgtc atgtttaagc ctcaataaaa ggggttaagc
     3181 cttgtgtgtc ttggcgcgtc aacaccatgg catcgccata gctgaagaag cggtattcgt
     3241 ggtctatcgc gtgttggtac aaagccatga ttgcttcata a
//
LOCUS       DAOMVK010000245         3278 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_8568_length_3278_cov_2.933602, whole genome shotgun sequence.
ACCESSION   DAOMVK010000245 DAOMVK010000000
VERSION     DAOMVK010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8568_length_3278_cov_2.933602"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1353
                     /gene="gltX"
                     /locus_tag="PK856_08240"
     CDS             <1..1353
                     /gene="gltX"
                     /locus_tag="PK856_08240"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRL02394.1"
                     /translation="YLHIGGVRTALFSWAFAKKHQGQFVLRIEDTDLERSTAESVQAI
                     LDGMHWVNMGYDEGPFYQTKRFDRYKEVIAKMLADGSAYHCYTTKEELDAQRAEAEAN
                     KQHFMYDGKWRPEAGKTLPAIPEGVPPVVRFKMPQDGVTAWDDAVKGRIEFNNKEFDD
                     LIIARADGTPTYNFCVVVDDFDMNITHIIRGDDHVNNTPKQINILKSLTDTMPVYGHL
                     PMILNANGQKMSKRRDAVSVVDYDRQGILPEALLNYLARLGWAHGDDEFFSMEEFVQW
                     FDLKDVSPSASRFDQEKLLWLNAQHIKAKTDEELVALVKPRLQVRDIENTDTPALAEV
                     LGLVKERAQELNALADECVYFYRKSTPTEEELKKHWDEEAPTRMHRFALQLEGLTDWT
                     AEAIHDLFKPFCEAEGIKMGKLGMPLRLAVCGTSKTPSIDAVLALLGRTEVLKRLSEV
                     "
     gene            1485..2054
                     /locus_tag="PK856_08245"
     CDS             1485..2054
                     /locus_tag="PK856_08245"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HRL02395.1"
                     /translation="MQLLQDKIRDHGNVLSPTVLKVDSFLNHQIDAALMKAVGEAFAE
                     RFKDTQATKILTLESSGIAPATMLGLVLGLPVIFARKRKSLTLSGNLLSATVHSFTKN
                     VTSEISVSRDFLQAGDQVIIIDDFLANGEAVRGLLSVVEQAEATTLGVGIVIEKGFQH
                     GGKELREKGVRVESLAIIDSMDTDHIRFR"
     gene            2368..2913
                     /locus_tag="PK856_08250"
     CDS             2368..2913
                     /locus_tag="PK856_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010327138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HRL02396.1"
                     /translation="MAQQHYHPVSRYIHWLMAAIFIIAWAIGFYSTEFMTYGVDANKG
                     SVITLHKNIASTILFLVVIRIFWRYTHPAPKLPDTMSAGMKKMAHFAHYVLYFLLLAI
                     PISGCIFSWSAGHPVPVLYSFEIPRLMAENKELVKYARPVHEYLSLFAGLVVVGHVLA
                     ALKHHFIDKGNVLRSMLKPKQ"
     gene            3169..>3278
                     /locus_tag="PK856_08255"
     CDS             3169..>3278
                     /locus_tag="PK856_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRL02397.1"
                     /translation="MNAQYLFTLPADSGEDFVAADHLPLVVYFYPKDSTPG"
BASE COUNT          856 a          861 c          740 g          821 t
ORIGIN      
        1 tatttacaca ttggtggtgt gcgcacggct ttgttttctt gggcttttgc caaaaaacac
       61 cagggtcaat ttgtactgcg catcgaagac accgatttgg agcgctcgac tgccgaatcg
      121 gtacaggcaa ttttggatgg catgcattgg gtgaacatgg gctatgacga aggccctttc
      181 taccaaacca aacgctttga ccgctacaaa gaagtcattg ccaaaatgtt ggcagatggc
      241 tctgcctacc attgctacac caccaaagaa gaattggacg cgcagcgcgc cgaggccgaa
      301 gccaacaaac aacattttat gtacgatggc aaatggcgtc ctgaagcagg caaaaccttg
      361 cctgccattc cagaaggcgt gccgcctgtg gttcgtttca aaatgcctca agacggcgta
      421 accgcttggg acgatgccgt taaaggccgc atcgaattca ataacaaaga atttgacgac
      481 ttgatcatcg cccgcgccga tggcaccccc acttacaact tctgcgtggt ggtagacgat
      541 tttgacatga acatcaccca catcatccgt ggcgacgatc atgtcaacaa cacccccaaa
      601 caaatcaata ttttgaaatc attgaccgac accatgcctg tgtacggcca tttgccgatg
      661 attttgaatg ccaacggtca aaaaatgtcc aaacgccgcg atgccgtgag cgtggtcgat
      721 tacgaccgcc aaggcatttt gccagaagcc ttgttgaatt atttggcgcg cttaggctgg
      781 gcccacggcg atgacgaatt cttcagcatg gaagaatttg tgcaatggtt tgatttgaaa
      841 gacgtgagcc cttctgccag ccgttttgac caagaaaaac tgttgtggct caatgcccaa
      901 cacatcaaag ccaaaaccga tgaagaattg gtggccttgg ttaaaccacg cttgcaagtg
      961 cgcgacattg aaaacaccga cacccctgct ttggctgagg tattggggtt ggtcaaagag
     1021 cgtgcgcaag aattgaatgc cttggccgat gagtgtgtgt atttctaccg caaatccacc
     1081 ccgactgaag aagaattgaa aaagcattgg gatgaagaag cccctactcg catgcaccgt
     1141 tttgccctgc aattagaagg tttaaccgat tggaccgctg aagccatcca tgacttgttc
     1201 aaacctttct gcgaagcaga aggtatcaaa atgggcaaat tgggcatgcc actgcgtttg
     1261 gccgtgtgcg gcaccagcaa aaccccgtct attgatgcgg tactggcctt gctcggtcga
     1321 accgaggtct tgaaacgcct gagcgaagta tagcaagctg cctgaaaaca cgcgcccacc
     1381 acgggcgcgt gtttgattta tgcgatttaa aaaccaacct gtttgattaa cagcgctgcc
     1441 accgccgtca aaagcgacaa cagcgtgctg aagcgataac cattatgcaa ttactacaag
     1501 acaaaatccg tgaccacggt aacgtactct ctcccaccgt attgaaagtg gattctttcc
     1561 tcaatcacca aatcgatgcg gccttgatga aagccgtggg cgaagcgttt gccgagcgtt
     1621 tcaaagacac ccaagccacc aaaattttga ccttggaatc gtcaggcatc gcccctgcca
     1681 ccatgttggg cttggttttg ggcttgccgg tgatttttgc gcgcaaacgc aaatctttga
     1741 ccttgtctgg caatttgttg tcggccaccg tgcattcgtt caccaaaaac gtgaccagtg
     1801 aaatctcggt atcgcgcgac tttttacaag cgggcgacca agtcatcatc atcgacgact
     1861 tcttggccaa cggcgaagcc gtgcgcggct tgctcagcgt ggtagaacaa gccgaagcca
     1921 ccactttggg cgtgggcatc gtgatcgaaa aaggcttcca acacggtggc aaagaattgc
     1981 gtgaaaaagg cgttcgagtc gagtctttgg ccatcatcga cagcatggac accgaccaca
     2041 tccgctttcg ttaaaccaac aagcggcctg taagcaagca cccgtgggta tctgtgaaga
     2101 tacccactta gcgtttacag gccgcatatc agaccttgca tacatccact gagacttgaa
     2161 tcgtcatcac tgtaatcacg ctgccgtatt gttctcgtca atcgtctctg ctgtactccc
     2221 ctccctaatt cccgcaacac tcagctccac caacttctcg agcttttatt aaccactggt
     2281 taataatatt tttcataatt aaccctgtat gaaaaatcaa gccactttat aatcctgccc
     2341 atctgtctta cacaaatgga aacccacatg gctcaacaac actaccatcc agtctcgcgc
     2401 tacatccact ggttaatggc agccattttc atcattgctt gggccatcgg tttttacagc
     2461 accgaattca tgacttacgg tgtcgatgcc aacaaaggct cggtcatcac cttgcacaaa
     2521 aacattgcgt ccaccatttt gtttttggtg gtcatccgca ttttttggcg ctatacccac
     2581 cctgcgccca aactgccaga caccatgtcg gcaggcatga aaaaaatggc acattttgcc
     2641 cattacgtgt tgtatttctt attgttggcg ataccgattt caggctgtat tttcagctgg
     2701 agcgcaggcc atcctgtgcc agtgttatac agttttgaaa ttcccagatt gatggcagaa
     2761 aacaaagaac tggtgaaata tgcgcgccct gtgcatgagt atttgtcttt gtttgcaggt
     2821 ttggtggtgg taggccatgt tttggctgcg ctgaaacacc acttcatcga caaaggcaat
     2881 gtcttgcgca gcatgttaaa accaaagcaa taaacccatt gaaataagcg gcctgtaaga
     2941 tttacaggcc gcttatttgt atgcccaaca acatgtatat ccattatcag ctcgcttgtt
     3001 ctgcccatca tggcgcgatt caactggctt ttaacaagcg ctcatagtct gattcaccgc
     3061 cttgtatcaa acaggtatta ctccataaat ccaacaagcg gcctgtacgc aatcaacaca
     3121 cacgaaagtg ttacaatgcc cactggtttt gattatggat gtccaaacat gaacgcccaa
     3181 tatttattca ctttgcctgc cgattctggc gaagattttg tcgctgccga ccacttgcct
     3241 ttggtggtgt atttctaccc caaagactcc accccagg
//
LOCUS       DAOMVK010000246         3263 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_8627_length_3263_cov_3.904613, whole genome shotgun sequence.
ACCESSION   DAOMVK010000246 DAOMVK010000000
VERSION     DAOMVK010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8627_length_3263_cov_3.904613"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PK856_08260"
     CDS             complement(<1..388)
                     /locus_tag="PK856_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HRL02398.1"
                     /translation="MTGRSSAAKNKQTLPMLPLRDVVVYPFMVIPLFVGRDKSIAALE
                     AAMEKDEPVFLLAQLNPSEEDPKANDLHKMGTIANVLQMLKLPDGTVKVLVEGVERAQ
                     VVSLNDTDQEFFVANIERVEEFQDDPV"
     gene            complement(539..1303)
                     /locus_tag="PK856_08265"
     CDS             complement(539..1303)
                     /locus_tag="PK856_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02399.1"
                     /translation="MNTRDQMVELAAVHGFVTYPTAIFCTDQGVGTRQSVGLVRDADV
                     HGLKQSLLLTEFEQQWHVTLSISVDLACFWSIMAEEMSSESVGYQDGLWAFLQENHVC
                     EERDGDDGLGDSVFLQSVEPLTDALLRPIGLGKKRLYDLQAASACVMDFEHLFLPWFE
                     ARQSLEAVLRLIKEGDATMMTALHAAHAERQRWFFKRKPPQYLEYESMMFALDAPVAE
                     FLQGVLLCCNGDVEAGTAVLLRYQQALQAKRRIFGG"
     gene            complement(1439..2731)
                     /gene="clpX"
                     /locus_tag="PK856_08270"
     CDS             complement(1439..2731)
                     /gene="clpX"
                     /locus_tag="PK856_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRL02400.1"
                     /translation="MSDKLKECSFCGKSENEVRNLIEGQQGCICNECVAACSVLFEQS
                     SDDEAVTPTEWSDEDLPTPAQMVVSLNDHVIGQDAAKKSLAVAVYNHYKRLRYPFAKQ
                     DEVELSKSNILLVGPTGSGKTLLAQTLARKLNVPFVMADATTLTEAGYVGEDVEQIIT
                     KLLSKCDFDVDKAQRGIVYIDEIDKIARKSENPSITRDVSGEGVQQALLKLVEGTVAS
                     VPPQGGRKHPNQEFIEIDTTNILFICGGAFDGLEKIIRRRSEKGGIGFGAEITSKDEG
                     KSVGELFKTVEPEDLIKFGLIPELIGRLPVITTLSELDEAAMIEILTEPKNALVKQYQ
                     VLFGMEGVELEFLPSALRSIAQLALARKTGARGLRSIMESVLLETMYVLPDLKHKGLK
                     KVIVNQEVIDKGATPELVYEGEAEAKPKRKTSSKKAAS"
     gene            complement(2728..>3263)
                     /locus_tag="PK856_08275"
     CDS             complement(2728..>3263)
                     /locus_tag="PK856_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRL02401.1"
                     /translation="RLLKERIIFMVGPVTDESANLVVAQMLFLESENPDKDIALYINS
                     PGGSVTAGLSIYDTMNLIKPDVSTLCLGQAASMGAFLLSAGTKGKRYALPNSRIMIHQ
                     PLLGGLSGQASDIEIHAKELMKIKQKLNELMAEHTGKRLAQLEKDTDRDNFMSADDAH
                     KYGLIDQVINSRDSIKA"
BASE COUNT          853 a          874 c          656 g          880 t
ORIGIN      
        1 ccacaggatc gtcttgaaat tcttccacac gctcaatatt ggccacgaaa aattcttgat
       61 cggtatcgtt taaagacacc acttgcgcgc gctccacgcc ttcaaccaac actttcaccg
      121 tgccatcagg cagcttcagc atttgcaaca cattggcaat ggtgcccatc ttgtgcaaat
      181 cgttggcttt tgggtcttcc tcgcttgggt ttaactgagc caataagaag actggttcat
      241 ctttttccat tgccgcttcc aaagccgcaa tcgacttgtc ccgtcccaca aacaacggaa
      301 tcaccatgaa tgggtaaacc accacatcac gcagtggcag cataggcaag gtttgtttat
      361 tttttgcagc agaagatcgt ccagtcatac tgtctcatct ttctatacag ggtttataca
      421 ttgttttgca aatgggtatc attggataaa attcaagccc tgtttgtgca atacacaaga
      481 gcgataggga agtatttgct tttgttatca attattgatt aaagggctta caggccgctt
      541 atccaccgaa aatacggcgc ttggcttgta aggcttgctg atatcgcaac aaaacggctg
      601 taccagcttc tacatcacca ttgcaacaca gcaatacacc ttgtagaaac tctgcaacag
      661 gtgcgtccaa ggcaaacatc atcgactcat attccaaata ctgtggtggt ttgcgcttaa
      721 aaaaccagcg ctggcgttct gcatgtgccg catgcaaagc cgtcatcatg gtcgcatcgc
      781 cctccttaat caaccgcagc acagcctcca agctttgcct cgcctcaaac catggtagaa
      841 acaggtgttc aaaatccatg acacaggctg atgcggcctg taaatcgtat aaacgcttct
      901 tccccaagcc tatgggtcgc aacaaagcat cagtcaatgg ctcgacggat tgcaagaaca
      961 cactgtcacc caaaccatca tcgccatctc gttcctcaca cacatgattc tcttgtaaaa
     1021 acgcccaaag accgtcttga taccccactg attctgaaga catctcttct gccatgatgc
     1081 tccaaaagca ggccaagtcg acactgatgc tcaaagtaac atgccattgc tgttcaaact
     1141 cggtcagcag cagcgattgt ttcaatccat gcacatcagc atcccgcacc aggccaacac
     1201 tttgtcttgt tcctacacct tggtctgtgc aaaatatggc tgtggggtat gtgacaaatc
     1261 cgtgtacagc tgccaattca accatttgat cgcgggtatt cattgattca atcacctatg
     1321 tttgtatggg tttacaggcc gcttattttc tcttgcaagc ggcctgtaaa ccgaaataga
     1381 acaatcatac aaaaaaacgc cacacaaggt ggcgttttca atacacaaac agtgcacatt
     1441 agctggcggc ttttttgctg ctggttttac gtttgggctt ggcttctgct tcgccctcgt
     1501 aaaccaattc aggtgtggca cctttgtcta tgacttcttg attcacaatg actttcttca
     1561 agcctttgtg tttcaaatca ggcaacacat acatggtttc gagcaataca gactccatga
     1621 tagaacgcaa accacgtgcg cccgttttgc gagccaaagc caattgggca attgaacgca
     1681 aggccgatgg caagaattcc aactccacac cttccatacc aaacaacact tggtattgtt
     1741 ttaccaaggc atttttaggc tcagtcagaa tctctatcat cgccgcttca tccaactcac
     1801 tcagcgtggt gatgacaggc aagcgaccta tcaactcagg aatcaaacca aatttaatca
     1861 agtcttcagg ctcaacggtt ttgaacaact cacccaccga tttgccttcg tctttgctgg
     1921 tgatttcggc accaaaacca atgccgcctt tttcagagcg acggcgaatg attttttcca
     1981 agccatcaaa cgcgccacca caaataaaca aaatattggt ggtatcgatt tcaatgaatt
     2041 cttggttagg atgtttgcgg ccgccttgtg gaggcactga agccaccgtg ccttcaacca
     2101 gtttcaacaa agcctgttgc acgccctcac ccgacacatc gcgggtgatg gatgggtttt
     2161 cactcttgcg ggcaatcttg tcaatttcat cgatgtaaac aatgccacgc tgcgctttat
     2221 ccacatcaaa atcacacttg ctcagtaatt tggtgatgat ttgctccacg tcttcgccga
     2281 cataacctgc ctcggtcaat gtggtggcat cggccatcac aaacggcaca ttcaacttgc
     2341 gcgccaaggt ttgtgcaagc aaagtcttac ccgagccagt tgggcctacc aacaagatgt
     2401 tagattttga caattccact tcatcttgtt tggcaaacgg gtagcgcaaa cgcttgtagt
     2461 ggttgtacac cgctactgcc aatgactttt tcgccgcatc ctgaccaatc acatggtcgt
     2521 tcaagctcac caccatttgt gcaggagtcg gcaaatcctc gtctgaccat tcggtaggcg
     2581 tgaccgcttc atcatcactg ctttgctcga acaacacgct acaggccgcc acacactcgt
     2641 tacaaatgca accttgctga ccttcaatca gattgcgaac ttcgttttcg ctcttaccgc
     2701 aaaacgagca ttcttttaat ttatcactca tgcttttatg ctatctcggc tgttaatcac
     2761 ttgatcgatc aaaccatatt tgtgcgcatc gtctgcagac atgaagttgt cacggtcggt
     2821 gtctttttcc aattgtgcca aacgcttgcc cgtgtgttcg gccatcaatt cattcaattt
     2881 ttgcttgatt ttcatcagct ctttggcatg aatttcaatg tctgaagcct gaccagacaa
     2941 accacccaac aaaggctgat ggatcatgat gcgactgttt ggcaaggcat aacgcttgcc
     3001 tttggtgccc gcagacaaca agaaagcacc catgctcgcg gcttggccca agcacaaggt
     3061 tgaaacatcg ggtttaatca gattcatggt gtcgtaaatc gacaaacctg cagtcacaga
     3121 gccacctgga gaattgatgt acaaagcgat gtctttgtct gggttttcac tttccaaaaa
     3181 cagcatttgc gccaccacca aattggcact ttcatcggtg acagggccta ccatgaaaat
     3241 aatgcgttct tttaacaagc gcg
//
LOCUS       DAOMVK010000247         3259 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_8639_length_3259_cov_2.626404, whole genome shotgun sequence.
ACCESSION   DAOMVK010000247 DAOMVK010000000
VERSION     DAOMVK010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8639_length_3259_cov_2.626404"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..712)
                     /locus_tag="PK856_08280"
     CDS             complement(53..712)
                     /locus_tag="PK856_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02402.1"
                     /translation="MGMSACASQYVKHELGAKQGHVAADYQYQVILADQLVAIGTPKT
                     QIANHADAWVLIGKQHSYLMTVSGKPTGYLQQVLHSVDARFLRIHGQYSRDGLGAVLI
                     AERPEQDKLFEQVRLDYRKPLAQMSAAERQRLQNLGFYCNAISSSGVYDCSLGLNVQY
                     HIITAAKPTGSKVHKFAQPHPFKITLRSVAIGAGGGRGDRTCAMAGRRRGARHWCTAI
                     V"
     gene            complement(904..2421)
                     /locus_tag="PK856_08285"
                     /pseudo
     CDS             complement(904..2421)
                     /locus_tag="PK856_08285"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
     gene            complement(2741..>3259)
                     /locus_tag="PK856_08290"
     CDS             complement(2741..>3259)
                     /locus_tag="PK856_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL02403.1"
                     /translation="TACVVVVLLSMPQLLIGTFGYDRADAFVLQSTGIVMQVIGCVLF
                     GWLADKIGGGKAMILGSIAVFVCALVYYNALGVWSAAAIFATYACLGLAAGAVGVVPM
                     LMVRMYPAPVRFTGISFSYNVSYAIVGGLTLPVVQWLSRYSDIGAMYYVLFLAALSVV
                     CCMVYMRTFERT"
BASE COUNT          910 a          888 c          713 g          748 t
ORIGIN      
        1 tgcggcctgt aagcctgtta caacacttac aggccgcttg attggcagcg cttcacacta
       61 ttgctgtgca ccaatgcctt gcaccacggc gccgcccagc cattgcacag gtgcggtcac
      121 cacgtccacc accagcgcca atggcaacgc tgcgcagcgt gattttgaac ggatgtggtt
      181 gggcaaactt gtgaacttta ctgcccgtag gtttcgcggc ggtgatgatg tggtattgca
      241 cattcaggcc gagcgagcaa tcgtatacac cgctgctgct gatggcattg caataaaagc
      301 ccaagttttg caaacgctga cgctcagctg ccgacatttg cgccaaaggc ttgcggtaat
      361 ccaaacgcac ttgctcaaac aatttatctt gttcaggccg ctcagcaatc aacaccgcgc
      421 ccaagccatc gcgtgagtat tgcccgtgta tgcgcaaaaa gcgcgcgtcc acgctgtgca
      481 agacttgttg taaatagcct gtgggtttgc cgctcaccgt catcaaatag ctgtgttgct
      541 tacctatcaa cacccacgca tctgcatgat ttgcaatctg ggttttgggt gtgcctatcg
      601 ccaccaattg atctgctaaa atcacttggt attggtaatc ggcggcaaca tggccttgct
      661 tcgcacccaa ttcatgtttg acatattggc tggcgcaggc gctcataccc atacaaatac
      721 tcaccgctgt tattaatgct ccgtgtttca ttgcaccccc tcaagacatg gtgcgaagta
      781 ttgatgtttg cgtgtgcact gtgcgataaa tgaattggtc catgctgcct gagcaagcgg
      841 cctgtaaaca ggttttcctc tttacaggcc gcttgttact cgcgcatggc tgtgtcattg
      901 catttagaag ttatttgggt cttctaattt tttcactttt gccgccaatt cagcatcgtt
      961 gatgtcggga atgccgtcgc catcaatgtc caccacttct tcaggcgcag tggccaaaca
     1021 ttctggattg tagacaatga tgttttcttt gccgtaagca tcttcagtga ccacgtattg
     1081 caaaccactg cggttcattt tgtggcattg gcgttggtac caacctgcaa aacctgtttt
     1141 cggctcgttt gggttgtgat aaaacgcgtt cattacccca ggcaatccaa ggccacacac
     1201 caaacccgaa agcagcacac aaataaatgt gtagccgatt aagatgtcgc tgtatttggc
     1261 caattgcggc atgttggcca ccgccaaaat caaggccaaa gaaatgcctc ctctgacccc
     1321 gccccaagac aagatggcca agctgccgtg gtagcttttt tgtttgaccc gtcgaaatgg
     1381 gatgaaggcc aaaaaattgg ccgcaaaccg cgccgcatgc aaaatcacaa atgccaccac
     1441 gccaccaatc aacaaatcgg catggaaatc gatgatgaac aattccatcc cgattaaggt
     1501 gaataagaag gaattgatga tgccctcaac cgtgtgccag aaatggttga cgtcattaac
     1561 ctcttgctca gacaaaatct cttgccaacg gttacccacc accaagccgc cgaccacaca
     1621 tgcaatcggg ccagacgcat gcgcatacaa agcgaccaaa tacgagcccg tggccaataa
     1681 ggcggtggac aagatgatgg attccatttc atgcttgcct ctgagcaagc gcaacaccat
     1741 ctaaccaaag gtccaaccaa tgacaacggc cacaacaatt tcataaccca aattgaccaa
     1801 agcgcctgcc acggtgaatt taccaccatt gatgaccgcc aaaatggtca tgaacaaggc
     1861 aatacacacc gcgtcattga acagcgattc gccttcgagt ttgaccatca aatgatgggg
     1921 tgcccgcacc gagctcagaa cgcctttgat gcctatcgga tctgtggcac ccaaggccgc
     1981 acccaataac aacaacacta acaaccccac tttgtgacct gtggccaatt ggtagccata
     2041 caggctcaaa ccaaagaaaa tggcacacaa aatcaaggct atgctggcca aaatagcgat
     2101 gggtctgaaa taggttctca aatccgagat tttgaatttc agtgccgatg aagtcagaat
     2161 gaaacaaatc accccattga tcagaaaatc atagaaattg atgttacgca ctgctttttc
     2221 aatttcagca atattgatgt gcacataatc attgtgccca aacaggctca ccgcccattg
     2281 cagtaaaaac acgaaaatgg ccgatacgat gggcacgcct atggccgcag gcaaacccaa
     2341 tacccgtttg ttgaacaagg tggtggcgat ggacaatgca aacaccaccg taaagacttg
     2401 taagaaagaa tagcttccca tgtgtgttgg ccccttttac ctttttattt tgtgacaatg
     2461 ttatcaagcg tccttgataa acagcggggt tattttaatc gaatttgagc gctattatca
     2521 ttattattta accgattctt ggcaagaact ctggttgtgg caaggtttgc ggtgtgtgtt
     2581 tttatacagc aatgccccca agctgcaatg cttgacagcc acatgccctg cacaacatcc
     2641 aaaatccaaa cagtaagaac gagcccaata cacacaaaag cggcctgtca ctttacaggc
     2701 cgcttgtctc atttcatgga gtattcaatg aatggctaag tcatgtgcgc tcaaacgtgc
     2761 gcatgtatac catgcaacac acgacactca atgctgccaa gaaaagtaca taatacatgg
     2821 caccaatatc gctgtatcgg ctcaaccact gcaccacagg caaggtcaag cctcccacta
     2881 tggcgtaaga cacgttgtaa gaaaaagaaa tgcctgtaaa gcgcacaggt gctgggtaca
     2941 tgcgcaccat caacatgggc acaacgccca ccgcgcctgc agccaaaccc aaacatgcat
     3001 aagtggcaaa aatggcagct gcgctccaca cacccaaggc gttgtaatac accaaagcgc
     3061 acacaaacac agcgatactg cccaaaatca tcgctttacc accaccgatt ttatcggcca
     3121 accagccaaa cagcacgcaa ccaatgactt gcatcacgat gcctgtggat tgcagcacaa
     3181 acgcatcggc gcggtcgtag ccaaaagtgc cgatgagcaa ttgcggcatg gacaacaaca
     3241 ccaccaccac acacgcggt
//
LOCUS       DAOMVK010000248         3251 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_8668_length_3251_cov_3.158010, whole genome shotgun sequence.
ACCESSION   DAOMVK010000248 DAOMVK010000000
VERSION     DAOMVK010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8668_length_3251_cov_3.158010"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="PK856_08295"
     CDS             <1..761
                     /locus_tag="PK856_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRL02404.1"
                     /translation="QGLPLVVDNTVATPLLCPALKLGADIVVESLTKYIGGHGNSIGG
                     AIIDGGKFDWAAHAERFPQLTTPDASYHGVNYAEHFGAAAFIARARVVPLRNMGAAIS
                     PMNTFLILQGLETLALRMERHCENALKVAQFLQNSGKVEWVNYPGLDNSPYKALIERD
                     FGGKASGLLSFGIKGGREAGARFIDALQLFTRLVNIGDAKSLACHPATTTHRQLNDAE
                     LKSAGVSADMVRLSVGIEHIDDLLADLEQALAAV"
     gene            1019..2446
                     /locus_tag="PK856_08300"
     CDS             1019..2446
                     /locus_tag="PK856_08300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HRL02405.1"
                     /translation="MQHTHKIWMSLAVVAWLSACAPMGQQKPLAHVLDATSLNLSSAP
                     LPHTQANFWQQLNDPVLNQLLAQTLSDAPDLAATRARIDGAAAQYGLARSQTGPQIAA
                     DGTFNRQRFSEYGMYAGLFGGQYMNSFDLSLKGAWEFDFWGKHRAQMAAAIGQKNALE
                     YELEQTRLMLAQAVLTQYVQWQNLLRQQDILQRRIAVQKNAAGLMVNREHAGLIAGSD
                     VYPLQMGAHQLQAQVAQIDGQIDAIKHTLAALSGQAPDALNGVQPTMWKAVPSVPAAS
                     VTADLLGQRPDIAAQRAALQAASEHIKAAKAEFYPNVKINGLVGLSSIEIGNLFETGA
                     KVVNILPAVTLPIFNSGALKAQLAQSHAQYDLQAAQYNKTVLTALREAADALSDGQVT
                     AKQSQERLAAWHLLQKASNAKAARVRAGLDNKLSTWQNQDDALAAEGLYQNALTAQRL
                     AWVNTQITLGGGFDAAANHTESAGK"
     gene            2448..>3251
                     /locus_tag="PK856_08305"
     CDS             2448..>3251
                     /locus_tag="PK856_08305"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRL02406.1"
                     /translation="MSDTPKPTESPAEHTLQAAAEPQIIAKPVGSKRKRNLIVLTLVL
                     LAIAAGFTAMYLLVWQHEVETDDSYVGGHLIQVTPQINGTVNAVVVDDTDTVKAGQVL
                     VELDADDAQLAYERAKSELANAAKQNQQLSAGVRQASAQIAVQQANLKKAQADLARRM
                     TLAGTDAISDEEIAHARSAVEAAQAGLTAAHAQEQQSKAALGRESLREQPAVQTAMSH
                     MKDAWLNLERTHIKAASDGQVARRNVQVGQKVAAGSPMMAIVPLNNLWVD"
BASE COUNT          802 a          843 c          893 g          713 t
ORIGIN      
        1 cacaaggctt gcctttggtg gtggacaaca ccgtggccac gccgctgttg tgcccggcat
       61 tgaaattggg cgcggacatt gtggtggaat ctttgaccaa atacattggt ggccatggca
      121 acagcattgg tggcgccatc attgatggcg gcaaattcga ttgggcggca catgccgagc
      181 gcttcccaca actcaccacg cccgatgcgt cttaccatgg tgtgaattat gccgagcatt
      241 ttggtgcggc agcgtttatc gcccgcgcgc gcgtggtgcc attgcgcaat atgggcgcag
      301 ccatcagccc gatgaacacc ttcttgattt tgcaaggctt ggaaaccttg gcgctgcgca
      361 tggagcgcca ttgtgaaaat gccttgaaag tggcgcaatt cttacaaaac agcggcaaag
      421 tagagtgggt gaattaccca ggcttggaca acagccctta caaagccctg atagagcgtg
      481 atttcggcgg caaggcttca ggcttgctca gctttggcat caaaggtggc cgcgaagcag
      541 gtgcgcgttt cattgatgct ttgcaattgt tcacgcgctt ggtgaacatc ggcgatgcca
      601 aatcattggc ctgccatcct gccaccacca cccaccgcca attaaacgat gccgagctga
      661 agtcagcagg cgtgagcgct gacatggttc gtttgagcgt gggcatagag cacattgacg
      721 acttgttggc tgatttggag caggctttgg cagcggtata aaaatcaaag atttgttcct
      781 ttagtcatgc aaatgcggcc tgtaaatcgg gccgcatttt tgtgtttgca cacatatcgc
      841 aaatagaatc aactgtctaa gaaaagtgca aaaaagggtt tgctggctct tggcaagtga
      901 ttttatctag agtagtgttc gagtttggct cggaatttgc cgtttttttt cattgttttt
      961 aatccataca aacacgtttg tatcttcctt gccgtcatga aaacataagg tgttacccat
     1021 gcagcacaca cataaaattt ggatgtcttt ggcagtggtc gcttggctca gcgcatgcgc
     1081 accgatgggg cagcaaaagc ccttggccca tgttttggac gcgaccagcc tcaatctgtc
     1141 atctgctcca ttgccacaca cccaagccaa tttctggcaa caactcaatg accctgtact
     1201 caatcaattg ttggcgcaaa ccttaagcga cgctcccgat ttggcggcga cccgtgcacg
     1261 cattgatggc gcagccgcac aatatggttt ggcgcgttcg caaacaggcc cacaaatcgc
     1321 cgcagatggc acgtttaacc gtcagcgttt ttctgaatac ggcatgtacg caggcttgtt
     1381 tggcgggcaa tacatgaaca gctttgattt gtcgctcaaa ggcgcgtggg aattcgattt
     1441 ttggggcaaa caccgagcgc aaatggccgc tgccatcggc cagaaaaatg ccttggaata
     1501 cgaattggaa caaacgcgat tgatgttggc gcaagcggtg ttgacccaat atgtgcaatg
     1561 gcaaaacctg ctgcgtcagc aagacatctt gcagcgccgc atcgcggtgc aaaaaaatgc
     1621 cgcaggcttg atggtgaacc gtgaacacgc aggcttgatc gctggcagcg atgtgtatcc
     1681 attgcaaatg ggtgcgcacc aattgcaggc acaagtggcg caaattgatg gtcaaatcga
     1741 tgccattaaa cacactttag cggccttaag cggccaagca cccgatgcct tgaatggtgt
     1801 gcaaccgacg atgtggaaag cggtgccctc cgtgcccgcc gcgagtgtga ccgcagattt
     1861 attgggccag cggcctgaca ttgcggcaca acgcgcggct ttacaggccg catctgagca
     1921 tatcaaagcc gccaaagccg agttttaccc caatgtgaaa atcaatggtt tggtcggctt
     1981 gtcgtcgatt gaaatcggca atctgtttga aacaggcgcg aaagtggtga atattttgcc
     2041 cgcggtgact ttgccaatat tcaacagcgg tgctttgaaa gcccaattgg cgcaaagcca
     2101 tgcccaatat gatttacagg ccgctcaata caacaaaacc gtgctcacgg ccctgcgtga
     2161 agcggccgat gccttgtcgg atgggcaagt cacggccaag caaagccaag agcgtttggc
     2221 ggcgtggcat ttgttgcaaa aagccagcaa tgccaaagct gcccgtgtgc gcgcaggttt
     2281 ggacaacaaa ttgagcacat ggcaaaacca agacgatgcc ttagcggccg agggtttgta
     2341 ccaaaacgcc ctcactgctc agcgcttggc ttgggtcaat acccaaatca ccttaggtgg
     2401 cggctttgat gctgctgcaa accacaccga atccgcagga aaataagatg agcgatacac
     2461 ccaaaccaac cgaatcaccg gcagaacaca ctttacaggc cgctgctgag ccacaaatca
     2521 tcgccaagcc cgtaggcagc aagcgcaaac gcaatttgat tgtgttgacc ttggtcttgc
     2581 tggccatagc agcaggtttc accgccatgt atttgttggt gtggcaacac gaagtggaaa
     2641 ccgatgattc ttatgtgggc ggtcacttga tacaggtcac gccgcaaatc aatggcacgg
     2701 tcaacgctgt ggtggtggac gacaccgaca ccgtcaaagc agggcaggtg ttggtggaat
     2761 tggatgccga tgatgctcaa ttggcgtacg aacgcgccaa gagcgaattg gccaatgcgg
     2821 ccaagcaaaa ccagcaattg agcgcaggtg tgcgccaagc ctcagcgcaa atcgccgtgc
     2881 agcaagccaa tttgaaaaaa gcccaagccg atttggcgcg ccgcatgact ttggctggca
     2941 cggatgcgat ttcagatgaa gaaatcgccc atgcgcgctc ggctgtggag gctgcgcaag
     3001 caggcttgac cgccgcgcat gcgcaagagc agcaatccaa agccgctttg ggccgcgaat
     3061 ctttgcgcga acagcctgct gtgcaaaccg cgatgtcaca catgaaagac gcttggctca
     3121 atctggaacg cacccacatc aaagccgcca gcgatggcca agtggcgcgc cgcaatgtgc
     3181 aagtgggtca gaaagtggct gcaggcagtc cgatgatggc cattgtgccg ctgaacaatc
     3241 tgtgggtaga c
//
LOCUS       DAOMVK010000249         3240 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_8716_length_3240_cov_2.430141, whole genome shotgun sequence.
ACCESSION   DAOMVK010000249 DAOMVK010000000
VERSION     DAOMVK010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8716_length_3240_cov_2.430141"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1289)
                     /locus_tag="PK856_08310"
     CDS             complement(<1..1289)
                     /locus_tag="PK856_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HRL02407.1"
                     /translation="MMNQELIDYTHQELIQHKQQIVADYVNSRQASMFFKRYGQELDR
                     CLGILWQEMFVNRRMCLLAIGGYGRQEMYPHSDLDLAIVCAESLSNEDEHDIARFVQI
                     LWDIGLAPAAKIGSTEQLLQAARDDLTSDTAFLEARFVCGNHKLAHEFVADLNLQRDV
                     AGFVEGKLLEQSQRHYKARGAASQLEPNIKTCPGGLRDLHTMLWLAKAQGLRPHFPTL
                     VHEGILTRAEAGLLMHSHKQLARMRIELHLVAGREEDRLIFDLQRQVAERLGYQDSES
                     SIKSEQLMKQMYRATKTVKQLNGILLPMLKGRVFSSLPRIVYDIDEKYYQVGNAIAVR
                     NVKLFKQQPHEIFNIVRVMQAHNDLSQMAPRTLRAWWQAAQKIGPNFYADNENRKMFI
                     SMFRRGTGLTHILRFFNLYGMLGRYIRPWAKIVGLLQ"
     gene            complement(1286..>1801)
                     /locus_tag="PK856_08315"
     CDS             complement(1286..>1801)
                     /locus_tag="PK856_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02408.1"
                     /translation="KILPPLAQLKQHKLLWDQTMTALSVAAMQKRPVNMSYALMCTYL
                     PVSLDKLHFDLRVPKDCADAAKVLQAVHPILLSTEPLQAETVLNVFQRCDAWRRPERW
                     QAAWEASLILAEVSQQEPPSQVWQQALMACLALDVAAIAAQSPDKASIPANIRAARLH
                     TITQSIASSTP"
     assembly_gap    1802..1817
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1818..2342)
                     /gene="cca"
                     /locus_tag="PK856_08320"
     CDS             complement(<1818..2342)
                     /gene="cca"
                     /locus_tag="PK856_08320"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="catalyzes the addition and repair of the essential
                     3'-terminal CCA sequence in tRNAs without using a nucleic
                     acid template; phosphohydrolase activities include
                     hydrolysis of pyrophosphate, 5'-nucleoside tri- and
                     diphosphates, NADP, and 2'-AMP with the production of Pi,
                     metal-dependent phosphodiesterase activity for 2',3'-cAMP,
                     2',3'-cGMP, and 2',3'-cCMP, and hydrolysis 2',3'-cyclic
                     substrates with the formation of 2'-nucleotides and
                     3'-nucleotides; these phosphohydrolase activities are
                     probably involved in the repair of the tRNA 3'-CCA
                     terminus degraded by intracellular RNases; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA tRNA nucleotidyl
                     transferase/2'3'-cyclic
                     phosphodiesterase/2'nucleotidase/phosphatase"
                     /protein_id="HRL02409.1"
                     /translation="MQVYLVGGAVRDGLLGLPIKDRDWVVVGATPEQMLSLGYTPVGK
                     DFPVFLHPSSHEEYALARTERKTSAGYHGFQFDTDTTVTLEQDLARRDLSINAMAQDA
                     QGLIVDPYGGQADLQAGILRHVSEAFAEDPVRILRVARFAARYDFQVADSTLQLMHTM
                     TSNGEVDALVAERVW"
     gene            2596..>3240
                     /locus_tag="PK856_08325"
     CDS             2596..>3240
                     /locus_tag="PK856_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017510038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRL02410.1"
                     /translation="MTSEIFMQLLLNGLVVGSIYGLIALGYTMVYGIMGLINFAHGEI
                     VMFGGMICISILTAFIGMGIQLPGFALVLLALICTIPICMAVGYTMELVAYRPLRRAP
                     RLAPLITAIGLSIVLQQVAVILWGTSYVKFPAVLDHTPINIMGAVVTKIQILIVVLCF
                     AIMFGMLAVVNKTKLGRAMRSTSQKPDVARLMGVNVNWVISITFMLGSGLGAIAG"
BASE COUNT          769 a          769 c          791 g          895 t
ORIGIN      
        1 tgctgtaaca gcccaacaat ctttgcccat ggtctgatgt agcgacctaa catgccatac
       61 aaattgaaaa agcgcagaat gtgggtcaag cccgtaccac gtcgaaacat agaaatgaac
      121 attttgcggt tttcgttgtc ggcgtaaaaa ttagggccaa ttttttgcgc cgcttgccac
      181 caagcacgca aagtgcgtgg ggccatttgg ctaagatcgt tatgcgcttg catgacacgg
      241 acgatgttga aaatttcatg cggctgttgc ttgaatagtt tcacattgcg aacggctatg
      301 gcattgccca cttggtagta tttttcatcg atgtcgtaca ctatgcgcgg caaactggaa
      361 aacacgcggc ctttaagcat gggcagcagt atgccgttaa gctgcttcac cgttttggtg
      421 gcacggtaca tttgcttcat caattgttcg cttttgatgc tggactcgct gtcttggtag
      481 cccaagcgct ccgccacttg gcgctgtaaa tcaaaaatca agcgatcttc ttcacgccca
      541 gcgactaaat gcaattcgat gcgcatccgc gccaattgct tgtggctgtg catcaacaag
      601 cccgcttcgg cacgggtgag gatgccttcg tgtaccaagg ttggaaaatg tgggcgcaag
      661 ccttgtgctt tggccagcca taacatggtg tgtaagtcgc gcaaaccacc tgggcaagtt
      721 ttgatgttag gttccaattg gctggcggcg ccacgggctt tataatggcg ctggctttgt
      781 tccaataatt tgccttctac aaaacccgcc acgtcgcgtt gtaaatttaa gtctgccaca
      841 aattcatggg ccaatttatg attgccacag acaaaacgtg cttctagaaa cgccgtatcg
      901 ctggttaaat catctcgagc ggcctgtaac aattgttcgg tactgcctat cttggcggca
      961 ggggccaaac ctatgtccca taaaatttgc acaaagcgcg caatgtcgtg ttcatcttca
     1021 ttacttaaag actctgcaca cacaatggcc aaatccaaat ccgagtgtgg atacatttct
     1081 tggcgaccat agccaccaat tgccaacaaa cacatgcggc ggttaacaaa catttcttgc
     1141 cacaaaatcc ccaagcatct gtctaattcc tggccgtagc gtttgaaaaa catcgaagct
     1201 tggcggctgt tgacatagtc tgcaacgatt tgctgtttgt gctgaatcaa ttcttggtgg
     1261 gtatagtcta tgagttcttg attcatcatg gtgtgctact cgctatgctt tgggtgatgg
     1321 tatgcaatct ggcggcacgg atgttggcgg gtatgctggc cttatcaggg ctttgcgcag
     1381 cgatggctgc cacatccagt gccaaacagg ccatcagtgc ttgttgccac acctgtgaag
     1441 ggggctcttg ttgggatact tctgccaaaa taagagatgc ctcccatgcc gcttgccaac
     1501 gctcagggcg acgccatgcg tcacagcgtt gaaacacatt caagactgtt tcagcttgca
     1561 gtggttccgt actaagtaag atgggatgca cggcctgtaa gactttggcc gcatcggcgc
     1621 agtctttggg tactcttaag tcaaaatgca gcttgtccaa tgagactggt aaataggtac
     1681 acatcaatgc ataagacatg tttacaggcc gcttttgcat cgctgcaaca ctcaatgcag
     1741 tcatggtttg atcccacaac agcttgtgtt gtttgagctg agccaatggc ggtaggattt
     1801 tnnnnnnnnn nnnnnnncca cacacgttcg gccaccaacg catcgacttc gccattgctt
     1861 gtcatggtgt gcatcaattg cagtgtgctg tcggccactt gaaaatcata gcgagcagca
     1921 aagcgggcca cgcgcaaaat gcgcacaggg tcttcagcga atgcctcact gacatggcgc
     1981 aagatgccgg cctgtaagtc agcttgtcca ccatagggat ccactatcaa cccttgcgca
     2041 tcttgtgcca tggcattgat gctgagatca cgtctggcca agtcttgttc taaggtgaca
     2101 gtggtatcgg tatcgaattg aaagccatgg tagcccgcgc ttgttttgcg ctcagtgcgt
     2161 gccaacgcgt attcttcatg gctgctaggg tgtaggaaaa cggggaaatc tttgccgaca
     2221 ggggtgtaac ccaaagacag catttgttca ggtgtcgcac ctaccaccac ccaatcccta
     2281 tctttgattg gcaaacccaa caaaccgtca cgcaccgcac cacctaccaa atatacttgc
     2341 atcttgccac ttcctgtttc ttgtcttacc ccaaagggat aaagtttgta tatttacaat
     2401 aagcatgtat attactgaca aattcattat ttgatgaatt ttttgcgatg cagcaacaga
     2461 ctctttgtta atttaatttt aattacgcaa agaaatgtgc tattttttcg acttattagg
     2521 gctcaatctg cgacataagc agaaacaccc cataaaaatc aatacaaaaa cgacacatgg
     2581 caaaggagcg agatcttgac gtcggaaatt tttatgcaac tgctgttaaa cgggctggtg
     2641 gttggctcga tttacggctt aattgcccta ggctatacga tggtgtatgg catcatgggt
     2701 ttgattaact tcgcgcacgg tgaaattgtg atgtttggtg gcatgatttg catcagcatc
     2761 ctgaccgcat tcatcggcat gggcatacaa ttgcctggtt tcgctttggt gttattggca
     2821 ctgatttgta ccattcccat ttgtatggca gtgggttaca ccatggaatt ggtggcttac
     2881 cgtccgttgc gtcgcgcacc acgtttggca ccgttgatta cggcgattgg cttgtctatc
     2941 gtcttacaac aagtcgctgt gattttgtgg ggcacttctt atgtgaagtt tcctgccgtt
     3001 ttggaccaca ccccaatcaa catcatgggt gcagtggtga ccaagattca aattctgatt
     3061 gtggtattgt gtttcgccat catgttcggc atgttggcgg tggtgaacaa aaccaaattg
     3121 ggccgcgcca tgcgctctac cagtcaaaag cctgatgtgg cgcgtttgat gggtgtgaac
     3181 gtgaactggg tgatttcgat tacctttatg ttgggttctg gcttgggtgc gattgcaggc
//
LOCUS       DAOMVK010000250         3204 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_8853_length_3204_cov_3.280406, whole genome shotgun sequence.
ACCESSION   DAOMVK010000250 DAOMVK010000000
VERSION     DAOMVK010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8853_length_3204_cov_3.280406"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..889)
                     /locus_tag="PK856_08330"
     CDS             complement(143..889)
                     /locus_tag="PK856_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02411.1"
                     /translation="MAGYRDLFIVSSALVTSYGLLPTSDRVQQTMNTLQSIRQWAPEA
                     DIVVLELSRERISDEYEQMFLQAGAKMVMHFFNHDGVMRNYQLCSPEKDPAGNYIKNL
                     NEITAMQAFLYWCLDKQYMQGYRRVFKISGRYSLCEPYNPELYEQADFKNRCAFLRLN
                     NSGLDFAVSGSFKYMYMTRLWSFDPVLLPKLEVWFEVMLDLLVKRYEVGGYTDIEHLL
                     AVVIPRKYCIYLDRVGVQGLIGLHGQKVVE"
     gene            complement(938..1282)
                     /locus_tag="PK856_08335"
     CDS             complement(938..1282)
                     /locus_tag="PK856_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003785859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HRL02412.1"
                     /translation="MKTAVVLSLLVMSNVLMTFAWYWHIKPGNSMMPMWKIVLISWCI
                     ALFEYCLAVPANHYGASWGIRPFQLKIMQEVITLTVFALFAVFYLKEGFKLNYLISFG
                     FILGAVYFAFKK"
     gene            complement(1342..1893)
                     /locus_tag="PK856_08340"
     CDS             complement(1342..1893)
                     /locus_tag="PK856_08340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRL02413.1"
                     /translation="MTTYKIGVVVGSLRKESINRQLAQALVKLLPQDFSTEFIEIGDL
                     PLYNQDLDGNQPAAVGKFKQQIEQQHGIIFVTPEYNRSIPGVLKNAIDHGSRPYGQNS
                     WAGKSVGIVGTSPGAPATSMSQQHLRNTLVFCNMPALNQPEAFIQWRDGMIDAQGSFN
                     ERTGSFMQNWVDAYVAWVKKTAA"
     gene            complement(1956..2498)
                     /locus_tag="PK856_08345"
     CDS             complement(1956..2498)
                     /locus_tag="PK856_08345"
                     /inference="COORDINATES: protein motif:HMM:NF017538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HRL02414.1"
                     /translation="MQTILYFHGFESNCQTEKFARTKAFFAAHGMELIGLDVNYADIA
                     PNVIDQIVAEIFSRHDIVACIGCSLGGYWANRSAVLHLVVAAVVNPALQFYPSYLQKY
                     PKLAAYQHESTYTESNLAPRLVMLGTADEVLDYRVAEAAFPESEVKIIEGGSHAGYDF
                     LDEFLQAAWLTIQQVDFCAA"
     gene            complement(2548..>3204)
                     /locus_tag="PK856_08350"
     CDS             complement(2548..>3204)
                     /locus_tag="PK856_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HRL02415.1"
                     /translation="REGKKAKSGDKDAQKLVAIMERLVAHLDQGLPVRSMGLDADELL
                     TIKTLCLLTVKPAMYVANVAEDGFENNPFLDALTELANKEGAPVVAVCAAMEAEIADL
                     DDADKAEFLQEMGLAEPGLNRLIRAGYDLLGLQTYFTAGVKEVRAWTIHKGDTAPQAA
                     GVIHTDFERGFIRAQVIAFDDYIALGGEAKAKEAGKMRAEGKEYVVADGDVINFLFNV
                     "
BASE COUNT          937 a          858 c          614 g          795 t
ORIGIN      
        1 aatccgctca ttgctgctgt ctttacaaaa cgatggtaga aaaaagagct ttcaggacgc
       61 ctgaaagcct caataagatt tatgctgggc accattttac aggatagatg ggcttggggt
      121 ctaacccaag ttaaccctac acctactcca ccaccttttg cccatgcaaa ccaatcaaac
      181 cttgcacccc gactctgtca agataaatgc agtatttgcg aggtatcacc accgccaaca
      241 aatgctcaat atcggtataa ccacccactt catagcgctt caccaacaaa tccaacatca
      301 cctcaaacca cacttccagt ttgggcaaca acacaggatc aaaactccac aaacgggtca
      361 tgtacatgta tttgaagcta ccgctaacgg caaaatccag acctgaatta ttcaaacgca
      421 aaaaggcaca ccgattctta aaatcggcct gctcatacaa ttctggattg tatggttcac
      481 acaaactgta gcggcctgaa attttgaaga cccgacggta gccttgcatg tactgcttat
      541 ccaaacacca gtacaaaaac gcctgcatgg cagtgatctc attcaaattt ttaatgtagt
      601 tgccagcagg gtctttttct ggtgagcaca attggtaatt gcgcattacc ccatcatgat
      661 tgaaaaagtg catcaccatt ttggcaccag cctgtaaaaa catttgttcg tactcatccg
      721 aaatacgctc acgcgacaat tccaatacca cgatatccgc ctctggtgcc cattgacgta
      781 tgctttgcag cgtattcatg gtttgttgca cccgatcaga agtgggcaac aaaccatacg
      841 aagtcaccaa tgccgacgat actatgaata aatcccgata ccctgccata ttttccccat
      901 taagccactg tcaggcctta ttgttgtgct taacccatca ttttttaaat gcaaaataca
      961 ccgcgcctaa gatgaaacca aagctgataa ggtaattcag cttaaaccct tctttcaagt
     1021 aaaacaccgc aaacaaggca aacaccgtca aagtgatgac ttcttgcatg attttcaatt
     1081 gaaacgggcg tatgccccaa gatgcaccat aatgattggc gggcacggcc aagcaatact
     1141 caaacaaggc gatgcaccaa gaaatcagca cgattttcca cattggcatc atcgaattgc
     1201 caggcttaat gtgccaatac caagcaaatg tcatcaacac attggacatg accaacaagc
     1261 ttaacactac cgctgttttc atcaacatac ttactcacat aaaaaaacgc gcccaaaagc
     1321 gcgttagctt acaaggcaaa ctcaagctgc ggtttttttc acccaagcca cgtatgcatc
     1381 cacccaattt tgcataaagc tgcctgtgcg ctcattaaac gaaccttgcg catctatcat
     1441 gccatcacgc cattggataa acgcttcagg ctgattcaac gctggcatgt tgcaaaacac
     1501 cagcgtattg cgcaaatgct gttgtgacat agaggtcgct ggcgcacctg gcgatgtacc
     1561 cacgatgcca actgacttac cagcccaaga gttttgaccg tagggacgcg aaccatggtc
     1621 tatcgcattt ttcaacacac caggtatgga acgattgtat tctggcgtca caaaaataat
     1681 gccatgttgt tgctcgattt gttgcttgaa tttgcccact gcagcaggtt gattgccatc
     1741 caaatcttgg ttgtacaaag gcaaatcgcc aatttcgatg aattcggtgc tgaagtcttg
     1801 tggcaacaat ttcaccaaag cctgtgccaa ttgacggtta atggattctt tacgcaaact
     1861 gcctaccaca acaccaattt tataagtagt catgtacacc ctttcaagaa tagcaacgag
     1921 aacggtatgg agaatgcctc cactgcaaag ttcatttaag ctgcacaaaa atccacttgc
     1981 tgtatggtca gccaagcggc ctgtaaaaac tcatccaaga aatcatatcc agcatgactg
     2041 cctccttcaa tgatttttac ttcactttct ggaaatgcag cctcagccac acggtaatcc
     2101 aacacctcat ccgctgtgcc caacatgacc aagcgaggag ccaaattgga ttcggtatag
     2161 gtagattcgt gttggtaagc cgcaagctta gggtattttt gcaaataaga tgggtaaaac
     2221 tgcaaagcag gattcaccac cgcagccacc accaaatgca acaccgcact gcgattggcc
     2281 caatagccgc ccaaactaca gcctatgcaa gcaacgatat catgacggct gaaaatttcc
     2341 gccacaattt gatcgatgac attgggggcg atatcggcat aattaacgtc taatccaatt
     2401 agttccatgc catgtgccgc aaaaaaagcc ttagttcggg caaatttttc ggtttggcaa
     2461 ttggattcaa acccatgaaa atacagtatg gtttgcatga aacacttcca aaacaaagcg
     2521 cctgtcataa acaggcgctg ttgttaatca cacattgaat aagaagttga tgacgtcgcc
     2581 gtcagccacc acatattctt tgccttcagc ccgcatttta cctgcttctt tggccttggc
     2641 ttcaccaccc aaggcaatgt agtcatcaaa cgcaatcact tgagcacgaa tgaaaccacg
     2701 ttcaaagtct gtgtgtatca caccagccgc ttggggtgcc gtgtcacctt tgtgtatggt
     2761 ccatgcacgc acctctttca cgccagcagt gaaataggtt tgcaaaccca acaagtcgta
     2821 acccgcacga attaggcgat tcaaacctgg ctctgccaag cccatttctt gcaaaaattc
     2881 ggccttatcg gcatcgtcca aatcggcgat ttccgcttcc atcgcggcac aaaccgccac
     2941 cacaggtgca ccttctttgt tggccaattc ggtcaaggca tccaaaaatg ggttgttttc
     3001 aaaaccatct tcagccacgt tggccacata catcgccggc ttaacagtca gcaagcacaa
     3061 ggttttgatg gtcaacaatt catccgcatc caagcccatt gaacgcacag gcaaaccttg
     3121 gtcaaggtga gccaccaaac gctccatgat ggccaccaat ttttgcgcgt ctttgtcgcc
     3181 tgatttggct tttttgcctt cacg
//
LOCUS       DAOMVK010000251         3193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_8906_length_3193_cov_2.654557, whole genome shotgun sequence.
ACCESSION   DAOMVK010000251 DAOMVK010000000
VERSION     DAOMVK010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8906_length_3193_cov_2.654557"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..152)
                     /locus_tag="PK856_08355"
     CDS             complement(<1..152)
                     /locus_tag="PK856_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRL02416.1"
                     /translation="MADISLLALFLIGFLGGSHCVGMCGGLSSAFVLQLPPNMRRWQL
                     ILCLNTG"
     gene            398..1231
                     /locus_tag="PK856_08360"
     CDS             398..1231
                     /locus_tag="PK856_08360"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRL02417.1"
                     /translation="MKILAWTALLCCGQLYAQAPERIVVLDAATADTLQALGVADAII
                     ALPKVQMPAYLAPVLREQVVDTGSLDKLAYSQLRQLKPDAIYALSLNAKTKSALQQIA
                     PTQVLAFNPQQYWQDLERNTLAVAVPHAKQSLAQQSLAHLQQQISAVQQQTNQDQRSV
                     VVLEHNQGHYRWEAQAAYQGLLYGVLQIKRPVNLPTQSTEVTQAQLQHWQADALWVID
                     ISGAKHQQSVDVSVLEEAARGTPALANERIKMLNPSHWTQTAVGLQGVQLQVDDVARM
                     W"
     gene            1271..1477
                     /gene="copZ"
                     /locus_tag="PK856_08365"
     CDS             1271..1477
                     /gene="copZ"
                     /locus_tag="PK856_08365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HRL02418.1"
                     /translation="MKHTTLKVQGMTCQHCVKAIEKSVGKLAGVTEVAVNLANAQVDV
                     HFDERQSDEAAIKAVIQEEGYEVV"
     gene            complement(1520..1879)
                     /locus_tag="PK856_08370"
     CDS             complement(1520..1879)
                     /locus_tag="PK856_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRL02419.1"
                     /translation="MSLLPSAEQICTHCGACCATFRVSFYWAEGSALPPDYTEALTPV
                     YDCMAGTNQSQPKCIALQGEVGREVSCSVYADRSSTCKEVQLGDEQCQKARLAHHLPP
                     IVFHESSNDDLHHDQAS"
     gene            complement(2101..3087)
                     /locus_tag="PK856_08375"
     CDS             complement(2101..3087)
                     /locus_tag="PK856_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRL02420.1"
                     /translation="MAHNHSHAHSHHSNNQKVLLWSFLIITAYMFIEAFGGWITNSLA
                     LLSDAGHMLSDALSLGIALLAFRYGVKAASRDKSFGYRRFEILAAALNGLTLMLVAAW
                     IVFEAVARFVSPLPIQSTGMLVIAVIGLIVNIVVAVMMFKGGDTHDNLNMRGAFLHVI
                     SDLLGSVAAILAALAVKWYGWTWADPLISVLVALLVLRSGYFVLRDALHILMEGTPRQ
                     IDTARIRALLLEQPEVTDIHDLHVWSITSGEHALASHVVLKQDQRLSEVQQLLHRLED
                     LLHAQGIGHVTLQVESQDHGHDANAVCSATPLQAAASVHEHDSHDHNHHIHP"
BASE COUNT          865 a          783 c          834 g          711 t
ORIGIN      
        1 cctgtattca aacacagtat caattgccaa cggcgcatgt tgggtggcaa ttgcaacaca
       61 aatgcactgc tcaagccgcc gcacatgccc acgcaatgac tgccgcccaa aaagccaatt
      121 aaaaacaatg ccaataaaga gatgtcagcc atgagcgtgc ttgttagacg aaaatcagca
      181 ttatagccaa gccgaccagt ctcggttcgg ccttaagtca aatcaaggac acatcgagac
      241 catggaattt aaaccaccgc attttgaatg gtggtaatat tttgttacat cttcattctt
      301 tgagcgtgag gcaacacatt taaacgcaag agccactgtg tttgcgtgtg ttggtttgga
      361 agccagttgc ttaaagcgtc accctgagga gatggccatg aaaatcttgg catggactgc
      421 gttattgtgc tgcggccaat tgtatgccca agcgcctgag cgcatcgtgg tgctggatgc
      481 ggccactgcg gacaccttac aggctttggg cgtggccgat gccatcattg ctctgcctaa
      541 agtgcagatg cctgcctatt tggcacctgt gttgcgtgaa caagtggtcg acactggcag
      601 tctggacaaa cttgcttaca gccaactgcg acaactcaaa cctgatgcca tttatgcact
      661 cagcctcaat gccaaaacca aatctgcctt gcaacaaatt gcgcccacgc aagtgttggc
      721 gtttaatccc caacagtatt ggcaagattt ggagcgcaat accctagcgg tggctgtgcc
      781 acatgccaaa caaagcttgg cacagcaaag cctggctcat ctacagcagc aaatatcggc
      841 tgtgcaacaa caaaccaatc aagaccaacg cagcgtggtg gtgttggagc acaatcaagg
      901 ccattaccgt tgggaggcac aagcagcata ccaaggcttg ttgtatgggg ttttgcaaat
      961 caagcggcct gtaaacttgc ccacgcaatc cactgaagtg acccaagcac aattgcagca
     1021 ctggcaagca gatgcgctgt gggtgataga catcagtggg gcaaaacacc agcaaagtgt
     1081 ggacgtgagc gttttggaag aggcggcacg tgggacgcct gcattggcaa atgagcggat
     1141 taaaatgtta aaccccagtc attggacgca aaccgccgtc ggtttgcaag gggtgcagtt
     1201 acaagtagac gatgtggcac gcatgtggtg aaaacctcaa tgaagacact ggcaaataag
     1261 gacaaaacaa atgaaacaca caacattgaa agtacagggc atgacttgcc aacattgtgt
     1321 caaagccatt gaaaaaagcg tgggcaaatt ggcaggtgtg acagaagtgg cggtgaattt
     1381 ggccaatgcg caggttgatg tgcattttga tgagcgccaa tcggatgaag ccgcaatcaa
     1441 agcagtgata caagaagagg gatatgaggt ggtctgagtt aagactagtt ttgcggacag
     1501 gcatgaaaaa agccagtggt taactggctt ggtcgtggtg taagtcatcg ttgctgcttt
     1561 catgaaacac aatgggcggc aagtgatgtg ccaaacgtgc tttttggcac tgctcgtcac
     1621 ccaattgcac ttctttgcac gtgctgctgc gatcggcata cacgctgcaa ctgacctcgc
     1681 ggcccacttc gccttgtaag gcaatgcact tgggctggct ttggttggtg cctgccatgc
     1741 aatcgtaaac tggggtcaat gcctcagtgt aatcaggagg taaggcactg ccttcggccc
     1801 aataaaaaga cacccgaaat gtggcacagc atgcaccaca atgggtacaa atctgttctg
     1861 ctgagggaag tagggacatg gttttccttg aagtggattg agagccaagc aaaaacacgc
     1921 aagcgagatt tttgccgagc cgcaatatta tgcagtgtca aacctcagga aaacaatggg
     1981 cagaaaagat ttattttttc ttacaaatca aatcaataga acaaaatatg cctttgctct
     2041 ttctgggtga aatcagcatg aacaaaggca tgaataggcg gatttatttg ggcttggtgc
     2101 tcaggggtga atgtggtgat tgtggtcatg cgaatcatgc tcatgcactg aggcagcggc
     2161 ctgtaaaggc gtggcactgc acacggcatt ggcatcgtgg ccatggtctt ggctttccac
     2221 ttgcagcgtc acatggccta tgccttgggc atgcaaaagg tcttctaagc gatgcagcaa
     2281 ttgttgcacc tcacttaagc gttggtcttg cttcaacacc acatggctgg ctaaggcatg
     2341 ctcaccactg gtgatgctcc acacatgcaa atcatggatg tcggtcactt caggttgctc
     2401 aagtagcaag gcacgaatgc gggccgtgtc gatttggcgt ggcgtgccct ccatcaaaat
     2461 gtgcagcgca tcgcgcagca caaaatagcc actgcgcaac accaacaagg ccaccaacac
     2521 actgattaag gggtcggccc aagtccagcc ataccacttc accgccagtg ctgccaaaat
     2581 tgccgccacc gagccgagca aatcgctgat gacgtgcaag aacgcgccgc gcatattgag
     2641 gttgtcgtgg gtgtcgccgc ctttgaacat catcaccgcc accacaatgt tcacaatcaa
     2701 accaatcacc gcaatcacca acatgcctgt gctttggatt ggcaaagggc tgacaaaccg
     2761 cgctaccgct tcaaacacaa tccacgcggc aaccaacatc agggttaagc cattgagcgc
     2821 ggccgcgagt atctcaaagc ggcggtagcc aaaacttttg tcgcgactgg cggctttcac
     2881 gccatagcga aacgccaata aggctatgcc aagcgacaag gcatcgctga gcatgtgacc
     2941 tgcgtccgac aagagcgcca agctgttggt aatccaaccg ccaaatgcct cgatgaacat
     3001 gtaggcggtg atgatgagaa acgaccacag caacactttt tgattgttgc tgtggtggct
     3061 gtgggcgtga gagtgattgt gagccatgtt tagtccttag aagcggcctg taagcctgct
     3121 tggaatcata gactcattgt gaagcatggg ctatgccact gccgtgtaaa acactgtgac
     3181 cattgtgttt ggg
//
LOCUS       DAOMVK010000252         3189 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_8928_length_3189_cov_3.704531, whole genome shotgun sequence.
ACCESSION   DAOMVK010000252 DAOMVK010000000
VERSION     DAOMVK010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8928_length_3189_cov_3.704531"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PK856_08380"
     CDS             <1..200
                     /locus_tag="PK856_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HRL02421.1"
                     /translation="PAYVVFPDRTLQNLVEAAPQRLVDLDGIYGMGSSKIDKYGAEVV
                     ALCQDIAADHSESVLNSASGL"
     gene            231..779
                     /locus_tag="PK856_08385"
     CDS             231..779
                     /locus_tag="PK856_08385"
                     /inference="COORDINATES: protein motif:HMM:NF022426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HRL02422.1"
                     /translation="MNTHDWLILSALTLLGACRTMTPEQCQQADWQRLGQVDGGNGQA
                     WSRLEQHQKSCQKAGIVPDVAAYQQGYQTGLQNYCQPQTIFNKAMQGMGNVSVCPADL
                     QADLFKFKQVPAAYREARDELERAERRYDNMQSNLYFSNNLTREQYLYYRQQLRFLRM
                     DVLEARTAVNWRASEVQRLKQQ"
     gene            complement(943..1614)
                     /locus_tag="PK856_08390"
     CDS             complement(943..1614)
                     /locus_tag="PK856_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRL02423.1"
                     /translation="MGDFDWTIILLAVVPVLFALTVQAVAQVLAARHYGDNTAVQMGR
                     LTLNPVPHIDLIGTIILPLFSVWMFATTGLPLIIGWVKPVPINYGNMRNIRMGLRMVA
                     ASGWIANFLMLIVWALICGISLHFGAAAEPVVKMAGIGIVVNIVLLAFGFIPLLPYAG
                     GRFIDSFLPAKYSIPFQKTAVWSGWLILALIVIQPLRPILMMYIMPIYSFFNAIAQFI
                     IKLFV"
     gene            complement(1640..2260)
                     /locus_tag="PK856_08395"
     CDS             complement(1640..2260)
                     /locus_tag="PK856_08395"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRL02424.1"
                     /translation="MAQFFTIHPDNPQERLVRQAADIIRDGGVVVLPTDSCYALVCAL
                     GDKKAMERIIAIRQLDLRHHFTLLCHNLSELGLYAKVDNSQFRLLKAATPGSYTFILP
                     ATKEVPSRTLHPKRKTIGLRVPDHAIAQAVLSEVGEALLSCTLMLPDDEEPLTDAYDI
                     RDRLEHHVDAVIDGGWCGTEATTVIDMTDGIELLRQGKGDVALFGL"
     gene            complement(2299..2610)
                     /locus_tag="PK856_08400"
     CDS             complement(2299..2610)
                     /locus_tag="PK856_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02425.1"
                     /translation="MNRKQLLLLQMASLLFGSLTLSHLLTGSMFSLFSALALVVTVAM
                     SVWGWRQIDAREQSMTPEQAELEAQNIHPYTPREISWLKGFAFFVGLMLLLIVWIQLG
                     K"
     gene            complement(2641..>3189)
                     /locus_tag="PK856_08405"
     CDS             complement(2641..>3189)
                     /locus_tag="PK856_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRL02426.1"
                     /translation="LRSGRITRLQLMSEKLEKKGIAGAYDGALALAGNPDSVGRAHLA
                     RYLVQAGHVKNMQQAFKKYLGDGKPAYVKHEWASLEDALAWITAAGGVAVIAHPARYD
                     MSATKMREFITEFKAAGGVGIEVASSSHTLSERLNYAMLAERFELYASAGSDYHALHE
                     GGQLGVPPELPPICKPIWELFH"
BASE COUNT          831 a          897 c          741 g          720 t
ORIGIN      
        1 tgccggctta tgtggtgttc cccgaccgca ccttgcaaaa cttggtcgaa gccgccccac
       61 aacgcctcgt cgatttggac ggcatttacg gcatgggcag cagcaaaatc gacaaatacg
      121 gggcggaagt ggtggctttg tgccaagaca ttgccgccga tcacagcgaa agcgttttaa
      181 acagtgcaag cggcctataa actgactttg ttactgagaa aagggttttc atgaacacac
      241 atgactggtt gatactttct gccctcacct tactcggcgc ttgccgcacc atgacacccg
      301 aacaatgcca acaagccgat tggcagcgct tggggcaagt ggatggcggc aatggtcagg
      361 cttggtcacg attggaacaa caccaaaaat cgtgtcagaa agccggcatt gttcccgatg
      421 tcgccgccta ccaacaaggc taccaaacag gtttgcaaaa ctattgccaa ccccaaacca
      481 tttttaataa agccatgcag ggcatgggca atgtgagtgt gtgccctgca gatttacagg
      541 ccgacttatt caaattcaag caagtgcctg cggcgtatcg cgaggccaga gatgaattgg
      601 aacgcgccga aagacgctat gacaacatgc aatccaacct ctatttcagc aacaatctca
      661 cgcgtgagca atacctgtat taccgccaac aattgcgctt cttgcgcatg gatgtactgg
      721 aggcgcgcac agccgtgaat tggcgcgcca gcgaggtgca gcgtttgaaa cagcaataac
      781 aattgcgctt cttacgcatg gatgtgctag aagctcgcac gcagcttaat tggcgtggca
      841 atgatgtgca gcgtttgaag cagcaatacc aattgtattg acgcgttacc tacaaaaaag
      901 cggcctgtaa atctttacag gccgcttttt atattgctgt catcacacaa acagcttgat
      961 gatgaactgt gctatcgcat tgaagaaaga gtaaattggc atgatgtaca tcatcaaaat
     1021 cgggcgcaat ggctgtatca caatcaaagc caaaatcaac caaccactcc acaccgcggt
     1081 tttttgaaac ggaatcgagt atttcgctgg caaaaagctg tcgatgaagc gaccacctgc
     1141 atacggcaac aatggaatga agccaaacgc caacaacacg atgttcacca caatgcctat
     1201 gccagccatt ttcaccacag gctcagctgc cgcgccaaaa tgcaaactga tgccgcaaat
     1261 caacgcccaa acaatcaaca tgaggaaatt ggctatccaa cccgaagccg ccaccatgcg
     1321 caaacccatg cggatgttgc gcatgttgcc gtagttaatc ggtacgggtt ttacccaacc
     1381 aatgatcagc ggcaaacccg tggtcgcaaa catccacacg ctgaacagcg gcaaaataat
     1441 cgtgccaatc aaatcaatgt gcggcacggg attcaaggtt aagcgtccca tttgcaccgc
     1501 tgtgttgtcg ccataatggc gcgcagccaa cacttgcgcc accgcctgta ccgtcaaggc
     1561 aaacaatact ggcaccaccg ccagtaagat tatcgtccag tcaaaatcac ccaaaatatg
     1621 tcttcctatg tatcaacttt tataaaccaa acagcgcgac atcgcctttg ccctgtcgca
     1681 acaattctat gccatcggtc atgtctatca ccgtggtcgc ttccgtgccg caccagccgc
     1741 catctatcac cgcgtccaca tggtgttcca agcggtcgcg aatgtcatac gcatcggtga
     1801 gcggctcttc atcatctggc aacatcaagg tacagctcaa taaagcctcg cccacttcgc
     1861 tcaacaccgc ttgcgcaatc gcatggtcgg gcacacgcaa accgatggtt ttgcgcttgg
     1921 ggtgcagcgt gcgcgatggc acttctttgg tggcgggcaa aataaacgtg taactgcctg
     1981 gcgtggccgc ttttaacaag cgaaactggc tgttgtccac tttggcatac agtcccaatt
     2041 cactcaaatt gtggcacaac aacgtgaaat ggtgacgcaa atccagttgg cgtatcgcaa
     2101 tgatgcgctc catcgccttc ttgtctccca aggcacacac caaggcataa caagaatcgg
     2161 tgggcaacac caccacgccg ccatcacgaa tgatatcagc cgcttggcgc accaaacgct
     2221 cttgcggatt gtctgggtgg atggtaaaaa attgggccat gttctgtttt cctcaagtca
     2281 ttgttggtgc ggcaggcttt atttgcccaa ttgtatccac actatcagta gcaacatcag
     2341 gcctacaaaa aaggcaaagc ccttcaacca tgatatttca cgtggcgtgt atgggtgaat
     2401 gttttgtgcc tccagctcgg cttgttcagg tgtcatgctt tgttcacgcg catctatctg
     2461 tcgccaaccc cacaccgaca tggccactgt caccaccaaa gccaacgccg aaaacaggct
     2521 gaacatcgag cctgtcagta aatgactcaa agtcaatgag ccaaacaaca ggctggccat
     2581 ttgtaacaac aataactgtt ttcggttcat gatgcggcct gtaaagtcaa tgtatcggtg
     2641 tcagtgaaat aattcccata tcggtttgca tatcggtggc aattcaggtg gcacacccaa
     2701 ttgcccgcct tcgtgcaaag catggtaatc gctgcctgca ctggcataca attcaaaacg
     2761 ctcggcgagc atggcgtaat tcaaacgctc gctcaaagtg tgtgagcttg atgcaacctc
     2821 tatgcccaca ccgcctgcgg ctttgaattc ggtgatgaat tcgcgcattt tggtggcact
     2881 catgtcgtag cgcgcagggt gggcgatcac cgccacgccg cctgccgcgg taatccacgc
     2941 cagcgcatct tctaagctcg cccattcgtg tttcacatac gcaggtttgc catcgcccaa
     3001 gtatttttta aacgcttgct gcatgttttt cacatgcccc gcttgcacca aatagcgcgc
     3061 caaatgcgca cgccccacgc tgtcaggatt gcctgccaaa gccaaagcgc cgtcatacgc
     3121 gcctgcgatg ccctttttct ccagcttttc cgacatcaat tgcaaacgcg tgatgcggcc
     3181 gctgcgcaa
//
LOCUS       DAOMVK010000253         3173 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_8991_length_3173_cov_3.528865, whole genome shotgun sequence.
ACCESSION   DAOMVK010000253 DAOMVK010000000
VERSION     DAOMVK010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8991_length_3173_cov_3.528865"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PK856_08410"
     CDS             complement(<1..634)
                     /locus_tag="PK856_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRL02427.1"
                     /translation="MEYLCLQAAFCHQQAACKPFDLPHQEIAMFKPLYAAFFALLLLC
                     GLSACSKPETALPAQTTVLILGDSLTQGVGASSAQTNYPELLKQQTRWNIINGGVSGN
                     TSAQALARLPELLQHHQPKLVIISIGGNDFLQRLSNSDTQANIAQSIALSQQSGAQVL
                     LVAVPELTLAAAVGHPNDHAMYAQLAQSHQVYLLEDAWSDVLGDEALRADP"
     gene            complement(647..1072)
                     /locus_tag="PK856_08415"
     CDS             complement(647..1072)
                     /locus_tag="PK856_08415"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRL02428.1"
                     /translation="MSSYQLHPVLTEKAHTAMPYTQFIGLEVWLDNQGSPLFHVPYKP
                     DNIGNTFLPALHGGMIGGFIESAATLFLRQHSGLDQFAKIIDFSIEYLRSGHPESTYA
                     ACTITRQGSRIAHLSVVVWQKDRERPIAVGRVHCLMPEA"
     gene            complement(1069..1533)
                     /locus_tag="PK856_08420"
     CDS             complement(1069..1533)
                     /locus_tag="PK856_08420"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRL02429.1"
                     /translation="MTEAVNAKVYSILSQHYFKLPHCQAIGLRFEHATHRSQTLSIDW
                     RSDLLGDQDTQVLHGGAITTLVDVSSATAVAAQLPDFETLATLDMRIDYLHAATPHLR
                     VFATSDCYRLSGQVAFVRTVCYHEDNINNPIALGTATFMRSPIPAHLKELIQ"
     gene            complement(1530..2363)
                     /locus_tag="PK856_08425"
     CDS             complement(1530..2363)
                     /locus_tag="PK856_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HRL02430.1"
                     /translation="MTEQATIELALQPEPAVAWVVGQPVADIPKDLFIPPEALQVILQ
                     NFEGPLDLLLYLIRKQNLDILDIPIAAITEQYLGYIAQLDSAHFDLAAEYLLMAAVLI
                     EIKSRLLLPQVKAVDEEEEIDPRQELAQRLLAYEQMRLAAMRLDELPQAGRDFAWAVL
                     HVEVDVPTVLPDVSVGDLKQAWLAILSRAKNHAQHQVVQDAISVRAQMSHVLRSLKLT
                     GSCRFSALFHEQQSVPLLVVNFIAMLELIKEGLIYVQQNAAFADIDIHLAATQAEESL
                     P"
     gene            complement(2356..3132)
                     /locus_tag="PK856_08430"
     CDS             complement(2356..3132)
                     /locus_tag="PK856_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HRL02431.1"
                     /translation="MDTEHNPNRRFGGIERLYGTPAWHKLQLAHVTVVGVGGVGSWVV
                     EALARSGVGHITLIDLDNVAESNVNRQLPAITPLFGMPKVDALKQRIFDINPACKVTT
                     IEDFADEDNIATLFAERVDFVVDAIDQVRVKAAMAAWFVRHKQPFVVSGAAGGQRHPE
                     HIRVADLSQVKNDKLLANLRYTLRKRHGFSRDTDKKMKVACVYSLETVMPPQNGDACD
                     VSGLQGLSCAGYGASMLVTATVGLHCAHAAIEHIVNAHHD"
BASE COUNT          731 a          778 c          921 g          743 t
ORIGIN      
        1 cggggtcggc gcgcaatgct tcgtcaccca aaacatcgct ccatgcgtct tccagcaaat
       61 acacttgatg gctttgtgcc aattgggcgt acatggcgtg gtcattgggg tggcccactg
      121 ccgctgccaa agtcaattcg gggacggcga cgagcaagac ttgtgcacca ctttgttggc
      181 ttaaggcgat gctttgggca atgttggctt gggtgtcgct attggataag cgttgcaaaa
      241 agtcgttgcc accgatgctg atgatgacga gcttgggctg gtggtgttgc aacagctcgg
      301 gtaaacgcgc cagagcctgt gcggaggtgt tgcctgatac accgccgttg atgatgttcc
      361 agcgggtttg ctgttttagc aattcaggat aattggtttg cgcagagctg gcgcctacgc
      421 cttgggtgag cgagtcacca agtatcaaca ccgtggtttg cgcgggtaag gcggtttctg
      481 gcttggagca ggcgcttaag ccacacagta gaagcaaggc gaagaaggcc gcataaagtg
      541 gtttgaacat ggcaatctct tgatggggta aatcaaatgg tttacaggcc gcttgctgat
      601 gacaaaaagc ggcctgtaaa cacaggtatt ccaagcgata aagtgcttaa gcctctggca
      661 tcaagcagtg cacgcggccc acggcgatgg ggcgctcacg gtctttttgc cacactacta
      721 ccgataaatg ggcaatgcgg ctgccttggc gggtgatggt gcaagcggca taagtcgatt
      781 cggggtggcc tgagcgcagg tattcaatcg agaagtcgat gattttggcg aattggtcga
      841 gtccactgtg ctgtctcaaa aacagtgtgg cagcgctttc aatgaagccg cctatcatgc
      901 cgccatgcaa ggcgggcaga aaagtattgc cgatgttgtc gggtttgtaa ggcacatgaa
      961 acaagggtga gccttggttg tccagccaca cttccaaacc gatgaattgg gtgtaaggca
     1021 tggcggtgtg ggctttttcg gttaaaacgg gatggagttg gtaagagctc attgaatcaa
     1081 ctcctttaag tgtgcgggaa tggggctgcg catgaaagtg gccgtaccca aggcgatggg
     1141 gttgttgatg ttgtcttcgt ggtaacacac ggtgcgcaca aaggcgactt ggccgctcaa
     1201 gcggtagcaa tcggaggtgg caaacacacg caaatggggc gtggcggcat gcaaatagtc
     1261 tatgcgcatg tccaatgtgg ccaaggtttc aaagtcgggc agttgggcgg ccacggcggt
     1321 cgcggatgac acgtcaacca aggtggtgat ggcgccgcca tgcaagactt gggtgtcttg
     1381 gtcgcccagc aaatcgctgc gccaatctat gctcaaggtt tggctgcggt gggtggcatg
     1441 ttcaaagcgc aggcctatgg cttggcaatg gggcaatttg aaatagtgct gactcaaaat
     1501 tgagtagact ttggcattga cggcttcggt catggcaaac tttcttctgc ttgggtggcg
     1561 gcaaggtgga tgtctatgtc ggcaaacgcg gcgttttgct gcacataaat caagccttct
     1621 ttgattaatt ctagcatggc gatgaaattc accaccaata agggcacgga ttgctgctcg
     1681 tgaaacaaag cgctgaagcg gcaagagcct gtgagcttca ggctgcgcaa cacatgcgac
     1741 atctgcgcgc gcacgctgat ggcatcttgc accacttggt gttgggcatg gtttttggcg
     1801 cgtgacaaaa tggccaacca agcttgcttc aaatcaccca cagacacatc gggcaaaacc
     1861 gtgggcacgt ccacttcgac atgcaagact gcccaggcaa aatcacgacc cgcttgcggc
     1921 aattcgtcca agcgcatcgc tgccaaacgc atttgttcat acgccaataa gcgctgtgcc
     1981 aattcttggc gtgggtcgat ttcctcttcc tcatccactg ctttcacttg tggtagtagc
     2041 aggcgtgatt tgatttcaat cagtaccgca gccatcagca aatactcggc cgccaagtca
     2101 aaatgggcac tgtcgagttg ggcaatatag cccaaatatt gctcggtaat cgcggcgatg
     2161 gggatgtcga gaatgtcgag gttttgcttg cgaatgaggt agagcagcaa atccagcggc
     2221 ccttcaaagt tttgcaagat gacttgcaag gcttcgggcg gaatgaacaa gtctttggga
     2281 atgtcggcaa cgggctgacc aaccacccaa gccacggctg gctcgggttg taaggccaat
     2341 tcgatggttg cttgctcagt catggtgggc attcacgatg tgttcgatgg cggcatgggc
     2401 acagtgcaaa cccacggtgg cggtgaccaa catgcttgcg ccatagcctg cacacgacaa
     2461 gccttgcaaa ccgctgacat cacatgcatc accattttgc ggtggcatca cggtttccag
     2521 tgaatacacg cacgcgacct tcattttctt gtcagtatca cgactgaaac cgtggcgttt
     2581 gcgcaaggta tagcgcaggt tggcaagcaa tttgtcgttt ttcacttggc tcaaatcggc
     2641 cacgcggatg tgttcgggat ggcgttgtcc acctgccgca cccgacacca caaacggttg
     2701 tttgtggcgc acaaaccaag ctgccatcgc ggccttaacc cgcacttggt ctatggcatc
     2761 caccacaaaa tccacgcgtt cggcaaacaa agtggcgatg ttgtcttcat cagcaaagtc
     2821 ttcgatggtg gtgactttac aggccggatt gatgtcaaaa atccgttgct ttaaggcatc
     2881 gactttgggc atgccaaaca agggcgtgat ggcaggcaat tggcgattga cgttggattc
     2941 ggccacattg tccaaatcga ttaaggtgat gtgacccacg ccagaacgcg ccaacgcttc
     3001 taccacccaa gaacccacgc cgcccacgcc caccacggtc acatgggcca gttgtagttt
     3061 gtgccaagca ggtgtgccgt ataagcgttc tatgccgccg aagcggcgat tggggttgtg
     3121 ttctgtatcc atcaatcagt taagccaagt caaataatcg gtaaatttta tgc
//
LOCUS       DAOMVK010000254         3168 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_9015_length_3168_cov_3.303566, whole genome shotgun sequence.
ACCESSION   DAOMVK010000254 DAOMVK010000000
VERSION     DAOMVK010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9015_length_3168_cov_3.303566"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PK856_08435"
     CDS             <1..383
                     /locus_tag="PK856_08435"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRL02432.1"
                     /translation="GVCDILFKQIAKTGTQFSVALLTAFVCAAVLIWGYLLSRKTEWH
                     GFSLRCGVILGALNFANIYFYIKAHQALHETPSVVFAGVNIGVLALAMLIGTVFWKER
                     LNKINLAGFGLAIAAVLCLYAAMG"
     gene            complement(442..858)
                     /locus_tag="PK856_08440"
     CDS             complement(442..858)
                     /locus_tag="PK856_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02433.1"
                     /translation="MKILLKLLVFILVYCWSGFATKMILLDVLPNYLPTETHPMHRLY
                     WAYLLWVSMACLLLLLMAGAWMVEIQLPQQCTNMALYVGLPLALVILVTSLFLLVVWV
                     FTFPCLLFALYLYHWHRQIKPNPSAKSALSIATKAD"
     gene            complement(977..1546)
                     /locus_tag="PK856_08445"
     CDS             complement(977..1546)
                     /locus_tag="PK856_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02434.1"
                     /translation="MDKLHKVHATSSNKVVLKRAKNTHPYPTAKVILTFAAAPAIAGM
                     AVFLLIALFSMQFGILIISPFVIFFAEALFLVPAVITGIIVSTCRWRKDDLDLIKAAA
                     VGGLSVLVSTMFFSPGAALLTSMIAAVVSLLLGYLVLPNAEYLDIPIYQHPEPDYWAV
                     EIPTLKDETQPTSAQTGFYYLKDLPQHKH"
     gene            complement(1670..2179)
                     /locus_tag="PK856_08450"
     CDS             complement(1670..2179)
                     /locus_tag="PK856_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02435.1"
                     /translation="MNTEYQTAYTPPAIAQVKGPVREPHLYPSFKVVLVFTSAPAMVG
                     LLLGLFTVFVTLKAEAVLALLVYVFVAELLYGLSALIAGIIINKCRWEKNAADICKAA
                     ITCAACAALPTALVDLEVALAMGLVAGVMGLILAAVVLPSAHSLASLALEAQATNPMT
                     PTSDKHHSD"
     gene            2387..3085
                     /locus_tag="PK856_08455"
     CDS             2387..3085
                     /locus_tag="PK856_08455"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRL02436.1"
                     /translation="MTEQWISVTSIDGFATDAFVVRPDVPAKAAVIVGMEIFGVNSHI
                     QAVCKRLANEGYVAIAPQLFDRIQKNVNMGYSADDVARGKELKQQAEADLKTEIASDI
                     QACIDWAYDHEGANKVGMMGFCWGGLNTWRAAQHAQSLWAAICYYGGGMVAENELADK
                     PVAPVLAHFSDNDATTPLDGIATLRERYGEEAVVYEYPAAHGFNCDQRGAYNQGAAEV
                     AWQRSLAFLRMCLQ"
BASE COUNT          835 a          701 c          849 g          783 t
ORIGIN      
        1 atggcgtgtg cgacatcttg ttcaagcaaa ttgccaaaac tggcacacag ttttcagtgg
       61 ccttgctcac agcatttgtg tgcgcggcgg tattgatttg gggctatttg ctcagccgca
      121 aaaccgagtg gcatggcttc agtttacgct gcggagtgat cttgggtgcg ctcaattttg
      181 ccaatattta tttctatatc aaggcacacc aagccttgca cgaaaccccg agcgtggtgt
      241 ttgcaggcgt gaacatcggc gtgttggctt tggccatgct catcggcaca gtgttttgga
      301 aagagcgttt gaacaaaatc aatttggcag gttttggctt ggcgattgcc gccgtcttgt
      361 gtttgtacgc ggccatgggc tgattttgaa gtgaaggaac agtttacagg ccgcttatgc
      421 ggcctgtaat cgttttgggc atcaatcggc ttttgtggca atggacagtg cagacttggc
      481 agaggggttg ggtttgattt gcctgtgcca atggtacaga tacaaggcaa acaacagaca
      541 aggaaaggta aacacccaca ccaccagtaa aaataaagac gtgaccaaaa tcaccaaggc
      601 caaaggcaag cccacataca aagccatgtt ggtgcattgt tgcggcagct gtatctccac
      661 catccacgcc cctgccatca gcagtaacaa cagacaagcc atgcttaccc agagcaaata
      721 cgcccaatat agtctgtgca tgggatgtgt ttcggtgggt aagtagttgg gcaatacatc
      781 caacaatatc attttggtgg caaagccaga ccaacaatac accaatataa acaccagtaa
      841 tttgagcagt attttcatga cttagccctt ggtgtgtgct gagtgcctat gcgccatgtt
      901 gctgattggt gatttacagg ccgcttgata tagatgcggc ctgtaaaggt attcatgtgc
      961 cttggttgta ctattatcaa tgcttgtgtt gtggtaaatc tttgaggtaa taaaaacctg
     1021 tttgagcgga cgttggttgg gtttcatctt tgagagtggg aatttccact gcccaatagt
     1081 caggctctgg gtgttggtaa atgggaatat ccaaatactc ggcattgggc agcaccagat
     1141 agcccaataa taaagacacc actgctgcaa tcatgcttgt gagcaaggct gcacctggtg
     1201 agaaaaacat ggtcgagacc aacacactca ggccaccgac tgctgcggct ttgatgaggt
     1261 ctaagtcgtc ttttcgccag cgacacgtgc tgacaatgat gcctgttatc acggcaggga
     1321 cgagaaacaa tgcttctgcg aagaaaatca caaacgggct gatgatcaga atgccaaatt
     1381 gcatggaaaa cagtgcgatg agcagaaaga cagccatgcc agcgattgca ggcgcggctg
     1441 caaaagttaa aatcaccttg gccgtagggt atgggtgggt gtttttcgcc cgcttgagta
     1501 ccactttatt ggacgatgtg gcatgcacct tgtgaagctt atccatatgc atctcctgag
     1561 tgagatttac aggccgcatt catcatgcgc agtagcgatt ggccttgtgt caccgtatta
     1621 aattctgcac aaaaaacatt ctgagcccac cgattcaaaa attcattgct caatctgagt
     1681 ggtgcttatc agatgttggt gtcattgggt ttgttgcttg ggcttccaat gcaagggaag
     1741 ccaatgagtg tgcagatggc aataccacgg ccgctaaaat gagtcccatc acgcctgcga
     1801 caaggcccat cgctaaagca acttctaagt cgaccagcgc agtgggtaag gcagcacatg
     1861 cggcgcaggt gatggctgcc ttgcagatat cagcggcatt tttttcccaa cggcatttgt
     1921 tgatgatgat gcccgctatc aacgccgata agccataaag cagctctgcc acaaacacgt
     1981 ataccagtaa agccaaaacc gcttctgcct tcaatgttac aaacacggta aacaaaccga
     2041 ggagcaggcc gaccatggct ggtgcggatg taaataccaa tacgactttg aacgaaggat
     2101 aaaggtgagg ttcacgcact gggcccttga cttgggcgat ggcaggcggg gtataagcag
     2161 tttggtattc ggtattcatg gcatgctcct atgggaatac aggcaacatt taaatctaga
     2221 gtttggattt ctttggtatt gagtttattt taatacattt atttctgttt taaaagaaat
     2281 ttatgtaatt tatacgtgcc gcttgttacg tacgcgaaaa gtgtgggcag actgaaattg
     2341 aggcaaaatg tgggctatta atgggaaaac agcataagga atggcgatga cagaacaatg
     2401 gatcagcgta acaagcattg atggttttgc caccgatgcg tttgtggtgc ggcctgacgt
     2461 gcctgccaaa gcggcagtga tagtgggcat ggagatattt ggcgtgaaca gccacataca
     2521 agccgtgtgc aaacgattgg cgaacgaggg ctatgtggcc atcgctccgc aattgtttga
     2581 ccgcatccaa aaaaacgtca acatgggcta cagcgccgat gatgtggcgc gtggtaagga
     2641 attaaaacaa caggctgaag cggacttaaa aactgaaatt gccagcgaca tacaagcctg
     2701 tattgattgg gcttacgacc atgaaggggc caacaaagtc ggcatgatgg gtttttgttg
     2761 gggcggcctg aatacttggc gcgccgcgca acatgcccaa agcctgtggg cggcgatttg
     2821 ttattacggc ggtggcatgg tggccgagaa cgaattggca gacaaacctg tggcacctgt
     2881 attggcgcat ttttccgaca acgatgccac cacgccgctt gatggcatcg ccacactgcg
     2941 agagcgctat ggcgaagaag cggtggtgta tgaatatcct gccgcacacg gctttaattg
     3001 cgaccaacgc ggggcttaca accaaggcgc agccgaggtg gcatggcagc gcagcttggc
     3061 ttttttgcgc atgtgtttgc aatgagcata aaccgaattt aacgcattat ggcggtacaa
     3121 taacggcttc ttgcggcgag aagccgctca ttgcagttga gaaagaca
//
LOCUS       DAOMVK010000255         3159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_9052_length_3159_cov_2.228737, whole genome shotgun sequence.
ACCESSION   DAOMVK010000255 DAOMVK010000000
VERSION     DAOMVK010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9052_length_3159_cov_2.228737"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /locus_tag="PK856_08460"
     CDS             <1..1198
                     /locus_tag="PK856_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HRL02437.1"
                     /translation="VPMYLAVPEITVTDAYFEAMSGLTTTGATVIKDIDALPPSINFW
                     RAMLQWLGGMGIIVLAVAVLPMLGVGGMQLYRAEMNGINKTNKLSPRIAQTAKTMWGI
                     YVFFTFVLWMALHFAGMSWFDALCHAMGSISLGGVTTHSDGLAFFNSPLIESIIMLGI
                     VVGGMNFANHYFAFANRSVAHYWRDSEIKLTLSALFFTILAGTIYLWLIKYYSFWESL
                     RYVAFTTVSIGLATGYSIVDFGAWPLVLSLWIMLLCNFLCNAGSTGGGIKAIRAIVLF
                     KFSLREMTLLLHPSAVYRVKINGNSVPERMALTILAFVFVYCLTIMLFTFIMMVTGLD
                     FLTSLTAIVACITTTGTGLGNVGPSYTFGYFSDLQKWLCLFVMLLGRLEIFTLLVLFT
                     PAYWRK"
     gene            1208..>2921
                     /locus_tag="PK856_08465"
     CDS             1208..>2921
                     /locus_tag="PK856_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRL02438.1"
                     /translation="MQNIFWRVSVLTAAVALAACSTQKETPVEPVTSSDASSLTGIQF
                     SRTPVENAGKVMNDYQQYQPTLDALRNDNDSAAMGFLNQQPHDSAMAEKVRNQWLKSL
                     GKREQWERFNAEYAKLDKNGAEQETQCFAHVSGVQKNPQLVQDLIKEHGKLPLGCNTM
                     LQSAASSGAVNQKQVWERVRGLLSNNQTTDARNLANAIGQPLPASLGSYSGSGAAAQE
                     SLLYSVTNANARKSNDIGSRISAISGSLTAEQAGFAYGAAGETHAKSQNFGVALQLFN
                     QADKAQLDTEQWEWYVRSALRFSRWQDVNNIIAAMPSSMQNKEDWQYWRGRALAATGQ
                     QGQAQGLYQKASKSGRNFYALMATEALGGKVNTQSNVKRASEAEQLRASQDGKIARAM
                     TLFQSSVSANDRTMRGVAQQQWRYAVRDLNEPSLLAAAAMAQKQGFNEMAIYAADRTN
                     NLIDYNIRYMSPYRDITSRYAAQVGIDPAWAYGLIRQESRFMIGARSHVGASGLMQLM
                     PATAREVERKIGSSSNGLDDIEHNIKLGTWYLADVRNSSGSETMATAGYNAGPGRARQ
                     WRANT"
     assembly_gap    2922..2931
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          772 a          809 c          762 g          806 t
ORIGIN      
        1 agtgcccatg tatctggccg tgcccgaaat cacagtgacc gatgcgtatt ttgaggccat
       61 gtcaggcctc accaccacag gcgccaccgt catcaaagac attgatgcct tgcctccatc
      121 catcaatttt tggcgcgcca tgttgcaatg gttgggcggc atggggatta tcgtattggc
      181 tgtcgcggta ttgcccatgt tgggtgtcgg agggatgcag ctctaccgcg ctgaaatgaa
      241 tggcatcaac aaaaccaata aactgtcacc tcgcatcgct caaaccgcca aaaccatgtg
      301 gggcatttat gtgttcttca cttttgtgct gtggatggct ttgcatttcg caggcatgtc
      361 ttggtttgat gccttgtgcc atgccatggg ttctatctcc cttggcggtg tgaccaccca
      421 ttcagatggt ttggcatttt tcaattcgcc tttgattgaa tccatcatca tgcttggcat
      481 cgttgttggc ggaatgaatt ttgccaatca ctattttgcc tttgccaacc gttcggttgc
      541 acactattgg cgcgattcag aaatcaagct taccctgtca gccttgtttt tcaccatctt
      601 ggcaggaacg atttacctgt ggttgattaa gtattacagc ttttgggaat cattgcgtta
      661 cgttgctttt accactgtgt ccataggctt ggccacaggt tattccatcg tggattttgg
      721 ggcttggccc ttggttttat cgctgtggat catgctgttg tgtaacttct tgtgtaatgc
      781 aggctcgaca ggtggcggca tcaaagccat ccgtgccatc gtcttattca agttcagttt
      841 acgagaaatg acacttttac tccacccaag cgcggtgtat cgggttaaaa tcaacggcaa
      901 ttcagtccca gagcgcatgg cactcaccat tttggcattt gtgttcgtgt attgtctcac
      961 catcatgttg tttaccttta tcatgatggt caccggattg gattttctca cttcattaac
     1021 cgcaatcgtg gcctgtatca ctaccacagg aacaggtttg ggcaacgtag gtcccagcta
     1081 cacgtttggg tatttcagtg atttacaaaa atggctgtgc ttgtttgtca tgctgcttgg
     1141 ccgtttggaa attttcacct tattggtgtt gtttacgccc gcttattggc gcaaataggg
     1201 aaaacttatg caaaacatat tttggcgcgt cagcgtcttg accgctgcag tcgctttggc
     1261 tgcctgttcc actcaaaaag aaacacctgt tgaacccgtt accagcagcg atgcctcgtc
     1321 tttaactggc atacagtttt ccagaacccc agtggaaaac gcgggcaagg tgatgaatga
     1381 ttatcaacaa taccaaccca cgcttgatgc actgcgtaac gataacgaca gtgctgcgat
     1441 gggtttttta aatcaacagc cacacgacag tgccatggct gaaaaagtgc gtaatcaatg
     1501 gctcaaaagc ttgggcaaac gtgaacaatg ggagcgtttt aatgccgaat acgccaagct
     1561 cgataaaaat ggtgctgaac aagaaaccca atgttttgcc catgtcagcg gtgttcaaaa
     1621 aaatccgcaa ctggtgcaag acttaatcaa agagcatggc aaactgcctt tgggctgtaa
     1681 caccatgttg caaagtgccg cttcaagtgg cgcggtgaat caaaaacaag tgtgggagcg
     1741 cgtgcgcggt ctgttgtcta acaaccaaac caccgatgcc cgcaatctgg ccaatgccat
     1801 aggtcaacct ttgcctgcca gcttgggaag ctattctggc agcggcgctg cggcgcaaga
     1861 atccttgctt tactccgtga ccaatgccaa cgcacgcaaa tccaatgaca ttggcagtcg
     1921 catctcagcc atttcaggca gcctcactgc cgagcaagca ggcttcgctt atggtgcagc
     1981 aggtgaaacc catgccaaga gccaaaattt tggcgtggcc ttgcaattgt tcaaccaagc
     2041 cgacaaagcc caattggata ctgagcaatg ggaatggtat gtgcgctctg ccctgcgctt
     2101 tagccgctgg caagacgtga acaacatcat cgccgccatg ccttcttcca tgcaaaacaa
     2161 agaagattgg caatactggc gcggtcgtgc cttggccgcc acaggtcaac aaggccaagc
     2221 gcaaggcttg taccaaaaag cctctaaatc aggccgcaat ttctatgctt tgatggcaac
     2281 cgaagccttg ggtggcaagg tcaataccca aagcaatgtc aaacgtgctt ctgaagccga
     2341 gcaactgcgt gcatcccaag acggcaaaat cgcccgcgcc atgactttgt tccaatccag
     2401 cgtatcggcc aacgaccgca ccatgcgtgg cgtggcacaa caacaatggc gctatgccgt
     2461 gcgtgatttg aatgaaccta gtttgcttgc agctgctgcc atggcacaaa agcaaggctt
     2521 caatgaaatg gcgatttatg ctgctgaccg caccaataat ctgattgatt acaacatccg
     2581 ctacatgtca ccttaccgtg acatcacaag tcgttacgcc gcccaagtgg gcatagaccc
     2641 tgcgtgggct tatggcttaa ttcgtcaaga aagccgcttc atgataggcg cacgctcgca
     2701 cgtgggcgcg tctggtttga tgcaattaat gccagccacc gctcgcgagg tggagcgcaa
     2761 aatcggctca tctagtaacg gcttagatga catcgaacac aacatcaaac tgggtacttg
     2821 gtatttggcc gatgtgcgca acagctcagg cagcgaaacc atggccactg caggctacaa
     2881 cgcaggccct ggtcgcgcgc gccaatggcg tgccaatacc cnnnnnnnnn naaaagtgat
     2941 ggccaatgca acgtattatg caagtttgtt caatgagccc aaacaatcat tgacccaacg
     3001 cataggtact gtgcctgcca agtaatcggt ttacacagca taaaaagcgg cctgtagatg
     3061 tttacaggcc gcttttgtat tgctagcaat gcttcgctct actggcaggc acaagcgctg
     3121 aaaagcgagt ggcaccatta tcagcctatt tttcacttc
//
LOCUS       DAOMVK010000256         3144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_9110_length_3144_cov_2.459048, whole genome shotgun sequence.
ACCESSION   DAOMVK010000256 DAOMVK010000000
VERSION     DAOMVK010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9110_length_3144_cov_2.459048"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="PK856_08470"
     CDS             complement(<1..922)
                     /locus_tag="PK856_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRL02439.1"
                     /translation="MKQLSVKACSDLLQSKQVSATELAQDYLQHIQNENAAINAFVHL
                     DADKTLHEAKLADERLQTGMSSLLTGVPIAYKDNFCQDGWVTSCGSKMLENFVAPYTA
                     HVVQNLRNKGMVTLGKVNMDEFAMGSTSETSHFGITRNPWNHGHVPGGSSGGSAAAVA
                     ARLTPAALGSDTGGSIRQPAAHCGVTGLKPTYGIISRFGMVAYASSLDQAGPIAQTAE
                     DCALLLNTMAGFDERDSTSLERPVEDYMRDLNQPLTGLRVGLPKEYLEGGLNADVTAT
                     VQNTVALLKSLGASMVDISLPQTEFVIPAYY"
     gene            complement(940..1227)
                     /gene="gatC"
                     /locus_tag="PK856_08475"
     CDS             complement(940..1227)
                     /gene="gatC"
                     /locus_tag="PK856_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRL02440.1"
                     /translation="MALTASDVQKIARLSRLALSDAELEATEKQLNNVFDMIAKMQTI
                     PTDGVQPMRHPHELALRLRDDTVSEENHREHYQECAPLVQAGLYLVPKVLD"
     gene            1460..2500
                     /locus_tag="PK856_08480"
     CDS             1460..2500
                     /locus_tag="PK856_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRL02441.1"
                     /translation="MLRFLTRFFSNDLAIDLGTANTLIYIKGKDIVLNEPSVVAMHIN
                     PNSNKDETLAVGADAKKMLGRTPGSIQAIRPMKDGVIADFNVTERMLKAFIRKVNNGK
                     LSAPPRIVICVPCGSTQVERKAIRDSALAAGASSVNLIEEPMAAAIGAGLPIEEPTGS
                     MVVDIGGGTTEVGVVSLSGLVYSHSIRVGGDAFDEAIINYVRRNYGMLIGEATAEQIK
                     KEIGTAFPGTEVREIEVKGRNLAEGIPRSFTITSNEVLEALSEPLNQIVQAVKNALEQ
                     TPPELGGDIAERGPVLTGGGALLRDIDRLLAEETGLPVNIADDPLTCVARGSGIALDK
                     IGKLNSIFVVNP"
     gene            2513..>3144
                     /gene="mreC"
                     /locus_tag="PK856_08485"
     CDS             2513..>3144
                     /gene="mreC"
                     /locus_tag="PK856_08485"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRL02442.1"
                     /translation="MSESLQFNQRRLNPLTKVVLLVLLASILLVTDKRVAAVKQMRSQ
                     LAGLFYPVQWIANQPISWYHDANQYFTSQNHIQEQNKLLSSENSRLKVELSQIKGIHN
                     NVDEIAANNRLKALLSLPTTEAQIVYVGNEPFPTHYLLNKGSSDGIKSGQAVVTADGL
                     LGQITSVGSKHSELTLLNNAQTVIPVMVARTGVRSLLYGNLETLELRYFPV"
BASE COUNT          797 a          777 c          753 g          817 t
ORIGIN      
        1 cataataggc aggaatcaca aattcggttt gtggcaagct gatgtctacc atgctcgcgc
       61 ccaaagactt gagcaaggcc acggtatttt gcaccgttgc cgtcacatct gcgttcaggc
      121 cgccttctaa atactctttg ggcaagccca cgcgcaatcc tgtcaaaggc tggttcaaat
      181 cacgcatgta gtcttcaact gggcgctcca agctggtgga atcgcgttcg tcaaagcccg
      241 ccatggtatt gagcaacaag gcgcaatctt cggccgtttg ggcaatcgga cctgcttggt
      301 ccaagcttga agcatacgcc accatcccga aacggctgat gatgccatag gttggtttca
      361 agccagtgac gccacaatgc gcagcaggct gtcgtatcga gccgcctgta tctgagccca
      421 aagccgcagg tgttaagcgt gcagccacag ccgccgccga accaccactg gagccaccag
      481 gcacatggcc atggttccat ggattgcggg taatgccaaa atgcgaggtt tcgctggtag
      541 aacccatggc aaactcatcc atattgacct tacccaaagt caccatgcct ttgttgcgca
      601 ggttttgcac cacgtgtgcg gtgtaaggtg cgacaaagtt ttccaacatt tttgagccac
      661 aagaagtcac ccaaccgtct tggcaaaaat tgtctttata ggcaatcggc acgccagtga
      721 gcaaactgct catgcctgtt tgcaaacgtt cgtctgccaa cttggcttca tgtaaagttt
      781 tgtcggcatc caaatgcaca aacgcattga tggcggcatt ttcattttga atgtgttgca
      841 aataatcttg tgccaattca gtggcggata cttgtttgct ttggagcaaa tcactgcatg
      901 ctttaacgct caattgtttc atctgtgtct ttcacgcgtt taatctaaaa ctttaggcac
      961 caaatacaaa ccggcctgta ccaaaggcgc acattcttga taatgctcac gatgattttc
     1021 ttcgctgact gtgtcatcgc gcaaacgcag cgccaattca tgtggatgac gcatcggttg
     1081 cacaccatcg gtgggtatgg tttgcatttt tgcaatcatg tcaaacacgt tgtttaattg
     1141 tttttctgtg gcttcaagct cggcgtcgct taaggcgaga cgggacagtc ttgcgatttt
     1201 ctgaacatca cttgcggtta acgccatgag ggtatcctta caattgcatt taaataatac
     1261 gttaaactgg cagccaaatg cttagcaagc ggcataaagt caggtatgat acacgctttt
     1321 ggttttaggc tgccgtgagc ggtaaaatta taaccgattt tcaccactgg gtaagcctta
     1381 gcacgagaca ccgcatcgca acagcgatgc ccacaatctg aatccatacg gcccaatata
     1441 acctttcagg aaacttccga tgttgcgttt tttaacccgc tttttctcca atgacctcgc
     1501 cattgacctt ggtaccgcca acactttgat ttacattaaa ggcaaagaca tcgttttgaa
     1561 tgagccttca gtggtggcaa tgcacatcaa ccctaattca aacaaagacg aaaccttggc
     1621 cgtaggtgcc gatgccaaaa aaatgctggg tcgtacccct ggctcgattc aagccatacg
     1681 ccccatgaaa gacggcgtga ttgccgactt caatgtgacc gagcgcatgt tgaaagcgtt
     1741 tattcgcaaa gtgaacaatg gcaaactctc tgccccgcca cgcattgtga tttgcgtgcc
     1801 ttgtggctct acccaagttg aacgcaaagc catccgtgat tcggctttgg cagcgggtgc
     1861 gtctagtgtg aacttaattg aagaacctat ggctgctgcc attggcgctg gcttgccaat
     1921 tgaagagcca actggttcta tggtggtgga cattggcggt ggtactactg aagtgggcgt
     1981 ggtgtctttg agcggcttgg tgtattcaca ttcgattcgt gtgggtggtg atgcgtttga
     2041 tgaagccatc atcaattatg tgcgccgtaa ctacggtatg ttgattggtg aagccactgc
     2101 tgagcaaatc aaaaaagaaa ttggtacagc cttccctggc actgaagtgc gtgaaatcga
     2161 agtcaaaggc cgcaatttgg cggaaggcat tccacgctcg ttcacaatca cgtccaatga
     2221 agtgttggaa gccttgtctg aacctttgaa tcaaatcgta caggccgtta aaaacgcctt
     2281 agaacaaacc cctcctgaat tgggtggtga catcgctgaa cgtggcccag ttttgactgg
     2341 cggcggcgcg ctgttgcgtg acattgatcg tttgctggca gaagaaacag gcttgcctgt
     2401 aaacattgct gacgatcctt taacctgtgt ggcccgtggc tcgggtattg ccttggataa
     2461 aattggtaag ttgaattcca ttttcgtcgt aaacccttga tgcgttgctt cgatgtctga
     2521 gtccttacaa tttaaccaac gacgcctcaa cccactgacc aaagtggtgt tgttggtgtt
     2581 gttggccagc attttgttgg ttactgacaa gcgcgtggct gcagtgaaac aaatgcgcag
     2641 ccaattagca ggtctttttt accctgtgca atggattgcc aaccaaccca tcagttggta
     2701 tcacgacgcc aatcaatatt tcacctcaca aaaccacata caagagcaaa acaaattact
     2761 cagcagtgaa aacagtcgtt taaaagttga actgagccaa atcaaaggca tacacaacaa
     2821 tgtggatgag attgcagcca acaaccgttt aaaagcactg ctgtcattgc ctaccactga
     2881 agcacaaatt gtgtatgtag gcaacgagcc tttccccacc cactatctgc tcaacaaagg
     2941 cagcagtgat ggcattaaat cgggacaggc tgtagtcact gcagacggct tattgggcca
     3001 aatcacttcg gttggcagca aacacagcga gttaaccctg ctcaacaatg cccaaaccgt
     3061 gataccggtg atggtggctc gcacaggtgt gcgcagtttg ctctatggca atttggaaac
     3121 cttggaattg cgttactttc ctgt
//
LOCUS       DAOMVK010000257         3133 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_9138_length_3133_cov_3.443145, whole genome shotgun sequence.
ACCESSION   DAOMVK010000257 DAOMVK010000000
VERSION     DAOMVK010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9138_length_3133_cov_3.443145"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1728
                     /gene="nrdA"
                     /locus_tag="PK856_08490"
     CDS             <1..1728
                     /gene="nrdA"
                     /locus_tag="PK856_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HRL02443.1"
                     /translation="KYPKDTRLGYVKRFYDAISEFKISLPTPIMSGVRTPSRQFSSCV
                     LIECDDSLDSINATASAIVKYVSQRAGIGINAGRIRALGSEIRGGEAQHTGCIPFYKL
                     FQTAVKCCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEENRVRHMDYGVQINRC
                     LYQRLLKNQDITLFSPSDVPGLYDAFFADQDEFERLYEQYEQDNSIRQRKVSAMDLFS
                     MLMQERAGTGRIYIQNVDHCNTHSPFNPAVAPVRQSNLCLEIALPTKPLDNVNDPEGE
                     IALCTLSAFNLGKIESMDDIEEWAELAVRALDALLDYQDYPIEAARQATMNRRTLGIG
                     VINYAYYLAKNGVRYSDGSALGLTHRTFEAIQFYLLKASVKLAQEYGACPKFGETTYS
                     QGVLPIDTYKKDLDAFCTEPLHLDWEGLRADIVKHGLRNSTLTALMPSETSSQISNAT
                     NGIEPPRGLVTVKASKDGILKQVVPEYERLKDDYELLWQMPNNDGYLKLVGVMQKFVD
                     QAISANTNYDPSRFEGGKVPMKQLIKDLLSAYKYGLKTLYYHNTRDGADDVQQDLQDD
                     GCAGGACKI"
     gene            1801..2934
                     /gene="nrdB"
                     /locus_tag="PK856_08495"
     CDS             1801..2934
                     /gene="nrdB"
                     /locus_tag="PK856_08495"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HRL02444.1"
                     /translation="MMYSTFSKKPNNQLLEPMFFGQPVNIARYDQQKYEIFEKLIEKQ
                     LSFFWRPEEVDVSQDRLDYSNLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPIVSI
                     PELETWIETWSFSETIHSRSYTHIIRNIVHEPATVFDDIVTNEYIIRRAEEIAQYYDD
                     LIKATQHYHLFGEGEHQVAGQTITVSLRELKRKLYRCMMCVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHILNLLRSGVDDPEMAEIAAECEQDCFDL
                     FKRAALQEKEWAEYLFKDGSMIGLNKDILGQYIEYITNLRMMAVGLQPAFEGANQNPI
                     PWINAWLSSDNVQVAPQEVELSSYLIGQIDSELSADDLGDFEL"
     gene            2934..>3133
                     /gene="yfaE"
                     /locus_tag="PK856_08500"
     CDS             2934..>3133
                     /gene="yfaE"
                     /locus_tag="PK856_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I ribonucleotide reductase maintenance
                     protein YfaE"
                     /protein_id="HRL02445.1"
                     /translation="MAVITTCDKSFALRENETLLEALERTGHDVEFQCREGYCGSCRT
                     KILKGSVSYQELPLAFVGSDEC"
BASE COUNT          833 a          855 c          731 g          714 t
ORIGIN      
        1 aaatacccta aagacacgcg cttgggctat gtgaagcgtt tttacgatgc gatttccgaa
       61 ttcaaaatct ctttgcctac gccgattatg tcgggtgtgc gcacgccatc acgccaattc
      121 tcttcttgcg tgttgattga gtgtgacgac agcttagaca gcatcaacgc caccgcctct
      181 gcaattgtga aatacgtgtc gcaacgcgca ggcataggca ttaacgcagg ccgcattcgc
      241 gccttgggca gcgaaattcg cggcggtgaa gcgcaacaca caggctgtat cccgttttac
      301 aaactgttcc aaaccgcagt gaaatgctgc tcgcaaggcg gcgtgcgcgg tggtgcagcc
      361 actttgttct accctatgtg gcatttggaa gtggaaagcc tgctcgtgtt gaaaaacaac
      421 cgcggcgtgg aagaaaaccg tgtgcgccac atggactacg gcgtgcaaat caaccgctgc
      481 ttgtaccaac gcttgctcaa aaaccaagac atcaccttgt tctcaccttc tgatgtgcct
      541 ggtttgtacg atgctttctt tgccgaccaa gacgaatttg agcgcttgta cgagcaatac
      601 gaacaagaca acagcatccg ccaacgcaaa gtatcggcca tggatttgtt ctcaatgttg
      661 atgcaagagc gcgcaggtac aggccgcatc tacatccaaa acgtcgacca ttgcaacacc
      721 cacagcccgt tcaacccagc ggtagcgcct gtgcgccaat ccaatttgtg cttggaaatc
      781 gccttgccta ccaaaccgct cgacaatgtg aacgaccctg aaggcgaaat cgccttgtgc
      841 accttgtctg cgttcaactt gggcaaaatc gaaagcatgg acgacatcga agaatgggca
      901 gaattggccg tgcgcgcttt ggatgccttg ttagactacc aagactaccc gattgaagcc
      961 gcgcgccaag ccacgatgaa ccgccgcacc ttgggcatag gcgtgattaa ctacgcttac
     1021 tacttggcca aaaatggcgt gcgctattcc gatggctcgg ccttgggctt gacccaccgc
     1081 acttttgaag cgattcaatt ttacttgctc aaagcctcgg tgaaattggc acaagaatac
     1141 ggcgcttgcc cgaaattcgg cgaaaccact tattcacaag gtgtgttgcc gattgacact
     1201 tacaaaaaag atttggatgc attctgcacc gaacctttgc atttggattg ggaaggcttg
     1261 cgtgccgata tcgtgaaaca cggcttgcgc aattccactt tgaccgcttt gatgccatct
     1321 gaaacctcta gccaaatctc caatgccacc aacggcattg agcctccgcg cggtttggtc
     1381 acagtcaaag cctcaaaaga cggcattttg aaacaagtgg tgcctgagta cgagcgcttg
     1441 aaagacgatt acgaattgtt gtggcaaatg cctaacaacg atggctattt gaaactggtg
     1501 ggcgtgatgc aaaaattcgt tgaccaagcg atttcggcca acaccaatta cgacccttcg
     1561 cgttttgaag gcggcaaagt gccgatgaaa caattgatta aagacttgtt gtctgcctac
     1621 aaatacggct tgaaaacctt gtactaccat aacacccgcg acggtgccga tgatgtacaa
     1681 caagacttac aagacgatgg ttgcgctggc ggagcctgca aaatctaagt ttgaacaggt
     1741 ttacaggccg cataaagcgg cctgtaagtt ctcagccaca acaatcaaaa agccaacacc
     1801 atgatgtatt ctactttcag caaaaaaccc aacaaccaat tgttggaacc gatgttcttc
     1861 ggccagcccg tcaacatcgc ccgttacgac caacaaaaat acgaaatttt cgagaaactc
     1921 atcgaaaagc aactgtcgtt cttttggcgt ccagaagaag tcgatgtgtc gcaagaccgt
     1981 ttggactaca gcaatttgcc cgagcatgaa aaacacattt tcatcagcaa cttgaaatac
     2041 caaaccctgc tcgactccat ccaaggccgc agccccaacg tggctttatt gcccatagta
     2101 tccattcctg aattggaaac atggattgaa acgtggtcgt tttccgaaac catccactcg
     2161 cgctcgtata cccacatcat ccgcaacatc gtacacgagc ccgccaccgt gtttgacgac
     2221 atcgtcacca acgaatacat cattcgccgt gccgaagaaa tcgcccaata ctacgacgac
     2281 ttaatcaaag ccacccaaca ctaccatttg tttggcgagg gcgaacacca agtcgcaggc
     2341 caaaccatta ccgtgtcttt gcgcgaactc aagcgcaagc tgtaccgctg catgatgtgc
     2401 gtgaacgcct tggaagccat ccgtttttac gtgtcgtttg cctgctcgtt tgcctttgcc
     2461 gagcgcgaat tgatggaagg caatgccaaa atcatcaaac tcatcgcccg cgacgaagcc
     2521 ttgcatttaa ccggcaccca acacattttg aatttgttgc gctcaggtgt ggacgaccca
     2581 gaaatggccg aaatcgccgc cgaatgtgag caagactgtt ttgacttgtt caaacgcgcc
     2641 gccttgcaag aaaaagaatg ggccgaatac ctgttcaaag acggctctat gattggtctg
     2701 aacaaagaca ttttgggcca atacatcgaa tacatcacca atttgcgcat gatggccgtg
     2761 ggcttacagc ccgcttttga aggcgccaac caaaacccga ttccatggat taacgcttgg
     2821 ctctccagcg acaacgtgca agtcgcgccg caagaagtgg aattaagctc ttacctcatc
     2881 ggccaaatcg actccgaatt gtcagccgat gatttggggg atttcgagct gtaatggccg
     2941 tcatcactac ttgcgacaaa tcgtttgcgc tgcgcgaaaa cgaaaccctg ctcgaagccc
     3001 tagagcgcac tggccacgac gtggaattcc agtgccgcga gggctattgc ggctcatgtc
     3061 gcaccaaaat cctcaaaggc agtgtcagct accaagagct gccactggct tttgtcggct
     3121 cagacgaatg ctt
//
LOCUS       DAOMVK010000258         3124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_9172_length_3124_cov_3.702183, whole genome shotgun sequence.
ACCESSION   DAOMVK010000258 DAOMVK010000000
VERSION     DAOMVK010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9172_length_3124_cov_3.702183"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="PK856_08505"
     CDS             complement(<1..878)
                     /locus_tag="PK856_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRL02446.1"
                     /translation="MTLPFSLWWLFLAILAILITASSIGSILALKYGSQNPTISNLNA
                     RINAWWVMTLVLVLAFVTGHIGSTFLFFLVSFAALRECLSLVYSRRGDYAVLVACFYV
                     VLPVQYYFVLTDWYGMFAIFIPVYAFLLLPILAGLSGDPTRFLDRTAKIQWSLMVSVF
                     CISHVPFLLNLKITNFDQNILLVIFLIAVVQASDVLQYIWGKLLGKRKIAPVLSPSKT
                     VEGFVGGVLSATALATALWWITPFTPFQAGLIGLVICLMGFAGGLVMSAIKRDFGVKD
                     WGHMIKGHGGMMDRVDS"
     gene            complement(875..1522)
                     /locus_tag="PK856_08510"
     CDS             complement(875..1522)
                     /locus_tag="PK856_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRL02447.1"
                     /translation="MKTGLSNPLARVLDTVLCLITSFITGVTSKQASDLAFTPTNKVY
                     YANHASHGDFVLVWISLPKTWRMLTQPVAGADYWLGSKLKAFIAEQVFRVLLIDRQHD
                     PKQAIAAMDGALKQGKSLILFPEGTRNTTDDQVLLPFKSGLYHLARENENIEFVPVWI
                     NNINRVLPKGKILPIPLLCDVRIGEVIKKLPDEDKTEFLTRTRQALLDLAPQEAK"
     gene            complement(1537..1947)
                     /locus_tag="PK856_08515"
     CDS             complement(1537..1947)
                     /locus_tag="PK856_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02448.1"
                     /translation="MSDAALYHTTAATLSSARINMAAAWLLAALAYMAILIAPFYLTV
                     YAAVFLATLLLASAYLHLRLHLDTVLLQALATGQLQDAEFEAALLQLRLRKKSLNHSA
                     HSRCVACLAVWYKALVIDSVLSLLSVITLVSTTL"
     gene            complement(1940..>3124)
                     /locus_tag="PK856_08520"
     CDS             complement(1940..>3124)
                     /locus_tag="PK856_08520"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HRL02449.1"
                     /translation="PFWAWSIVPYWTLNLFYAGAFFCCQNRAQIHRLLQQLVLAQAIA
                     VVCFIAYPLTFTWEKPLTDGLTGRLFSALAGFDQPYNQAPSLHIILTMVIGRFYWYRF
                     AMWTRPLWLAWLLLIAVSVLTTYQHHFIDIPTGMLVGCLVLWLLPWQGNQAASFAVFR
                     LPSNPFLSLPKPKRSPTHLRWVWLYASCAVMLSLLAFGLQAAWYGLLWPALALLWLAT
                     VYGHADAAAWQKQATGKHTLAVSLFAWPLRLMYRANMAYWLRGKALSAQVTERIHVGS
                     LLAAPDFHAVVDVSAEYPLAVMPSYYATIPMLDMVPADVAALRAAADALHQMLQQQNQ
                     PVLVCCALGYSRSVAVVLVYLLRQGHAHDLQAALKLLQQARPDMVVSPDHQQQILKAA
                     HE"
BASE COUNT          843 a          817 c          842 g          622 t
ORIGIN      
        1 gaatcgaccc tgtccatcat gccgccgtgg cctttgatca tatggcccca gtctttcacg
       61 ccaaagtcgc gtttgatggc cgacatcacc aagccgcctg caaagcccat caagcaaatc
      121 accaagccga ttaagcctgc ttgaaatggt gtgaacgggg taatccacca caaagccgtg
      181 gccaaagccg tggccgacag cacgccgccc acaaagcctt ccacggtttt cgagggcgac
      241 aacacgggcg cgattttgcg cttgcccaag agcttgcccc aaatgtattg caacacatca
      301 ctggcttgca ccaccgcaat caagaagatt accaacaaga tgttttggtc gaaattggtg
      361 attttcaagt tcaataaaaa tggcacatgc gaaatacaga acacgctcac catcaacgac
      421 cattggattt tggcggtgcg atcaaggaag cgggtagggt cgcctgacaa gcctgctaaa
      481 atcggcagca gcaagaaggc gtacacgggt atgaagatgg cgaacatgcc ataccaatcg
      541 gtgagcacga aatagtattg caccggcaag acgacataaa aacacgccac caacacggca
      601 tagtcgccac ggcggctgta aaccaagctc aaacactcgc gcaaagcggc gaaagacacc
      661 aagaaaaaca aaaatgtaga accgatgtgg cccgtcacaa atgccaacac caataccaaa
      721 gtcatcaccc accatgcgtt gatgcgggcg ttcaaattgc tgatggtcgg gttttgcgag
      781 ccgtatttca gcgccaaaat cgagcctatg ctgctggcgg taatcaaaat cgccaatatg
      841 gccagaaaca gccaccacag tgaaaaaggt aaagtcattt tgcctcctgc ggtgccaaat
      901 ccaacaaggc ttggcgggtg cgggttaaga attcggtttt gtcttcatcg ggcagttttt
      961 taatcacctc gccgatgcgc acatcgcaca gcaagggaat gggcaagatt ttgcctttgg
     1021 gcaagacgcg gttgatgttg ttaatccaaa cgggtacgaa ttcaatgttt tcgttttcgc
     1081 gcgccaaatg atacaggccg cttttgaatg gcaataaaac ttggtcatcg gtggtgttgc
     1141 gcgtgccttc tggaaacaga atcagcgact tgccttgttt gagcgcaccg tccatggcgg
     1201 cgatggcttg tttggggtcg tgctgtctgt ctatgagcaa tacccgaaat acttgttcgg
     1261 caatgaaagc cttgagtttg ctgcccagcc aataatcggc acccgccaca ggttgggtga
     1321 gcatgcgcca ggttttgggc aaggaaatcc acaccaacac aaaatcgccg tggctggcgt
     1381 ggttggcata atagacttta ttggtggggg taaaggccaa atcactggct tgtttggaag
     1441 tgacgcccgt gatgaacgag gtaatcagac acagaacagt gtccaaaacc cgtgccaatg
     1501 ggttggagag tccggttttc atgcatgctc ctgcttttag agtgtggtgc tgactagagt
     1561 gatgacactc agcaaggata acacactatc tatgacaagc gctttgtacc acaccgccaa
     1621 acaggcaaca cagcggctgt gagcgctgtg attgaggctt tttttgcgca aacgcaattg
     1681 caataaagcg gcttcaaatt cagcgtcttg caattgccct gttgccaaag cctgcaatag
     1741 cacagtatcc aaatgcaggc gcaggtgcaa ataagccgat gccaacaaca atgttgccaa
     1801 aaataccgct gcgtacaccg tcaaataaaa cggagcaatg aggatggcca tgtaagccaa
     1861 tgccgccaac aaccaagccg ccgccatgtt gatgcgggcg ctggataagg tggcggcggt
     1921 ggtgtggtag agtgcggcat cactcatggg cggctttcag tatctgttgt tggtggtcag
     1981 gggaaaccac catgtcaggc ctggcttgtt gtaacagctt caaagcggcc tgtaaatcgt
     2041 gcgcatggcc ttgacgcagc aaatacacca acaccacggc cacgctgcgg ctatagccta
     2101 aggcacagca caccaacaca ggctggtttt gctgttgcag catttgatgc aaggcatcgg
     2161 ccgcggcgcg caaagccgca acatcggcag gcaccatgtc caacatggga atggtggcgt
     2221 aataactggg catgaccgcc aacgggtatt cggcgctcac atcgaccacg gcgtgaaaat
     2281 caggcgcagc caataagctg ccgacatgga tgcgttcggt gacttgggca gacaaagcct
     2341 tgccgcgcag ccaatacgcc atgttggcgc ggtacatcag gcgcaaaggc caggcaaaca
     2401 ggctcaccgc caaagtgtgt ttgccagtgg cttgtttttg ccacgctgcc gcatcggcat
     2461 ggccgtacac cgttgccaac cacaacaaag ccagcgcagg ccacagcaag ccataccaag
     2521 cggcctgtaa gccaaatgcc aacaggctta acatcacggc gcaactggca tacagccata
     2581 cccagcgcaa atgggtgggg ctgcgtttgg gtttgggcaa ggacagaaac ggattgctgg
     2641 gcaagcgaaa cactgcgaat gacgcggctt gattgccttg ccaaggcaag agccataaga
     2701 ccaaacagcc caccaacata cctgtaggga tgtcgatgaa gtggtgttgg taagtggtga
     2761 gcaccgacac tgcaatgagc agcagccatg ccagccacag cggccttgtc cacatcgcaa
     2821 agcgatacca ataaaagcgc ccgatgacca tggtgagaat gatgtgtaaa gacggcgctt
     2881 ggttataagg ttggtcaaaa cctgccagtg cgctgaacaa gcggcctgta aggccatcgg
     2941 tgagcggctt ttcccaagtg aaggtcagag ggtaggcgat gaaacacact accgcgatgg
     3001 cctgagccag taccaattgt tgcagcaagc ggtggatttg tgcgcggttt tgacagcaaa
     3061 aaaacgcgcc tgcgtaaaac agattcagcg tccaatacgg cacgatgctc caagcccaaa
     3121 acgg
//
LOCUS       DAOMVK010000259         3106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_9234_length_3106_cov_2.529990, whole genome shotgun sequence.
ACCESSION   DAOMVK010000259 DAOMVK010000000
VERSION     DAOMVK010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9234_length_3106_cov_2.529990"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(289..672)
                     /locus_tag="PK856_08525"
     CDS             complement(289..672)
                     /locus_tag="PK856_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRL02450.1"
                     /translation="MSKQIIHTDKAPAAIGAYSQAVRAGNTVWISGQIPMDPATGDVV
                     AGGFAEQTEQVFKNLAAVIEAAGGTFDQVVKINAYLTDLANFATFNETMARYMSQPYP
                     ARAAVGINSLPKGVLVEAEAILVLE"
     gene            complement(694..1863)
                     /gene="hemW"
                     /locus_tag="PK856_08530"
     CDS             complement(694..1863)
                     /gene="hemW"
                     /locus_tag="PK856_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HRL02451.1"
                     /translation="MIEFHPIGSGLTALPPLSLYVHLPWCVQKCPYCDFNSHQSSSEG
                     LPEEAYIQALLQDLEHELPHIWGRSVETLFIGGGTPSLFQAASIDKLLAGIRARVRLL
                     PNAEITMEANPGTFERERFAGFAAAGVNRLSLGVQSFQPEHLQALGRIHDDQQAITAI
                     EAACKLFPRFNVDLMYALPKQSIEQAVRDVQTAIDLGAPHISAYQLTLEPNTAFGHTP
                     PKNLPDDDALIDIEEAVHATLLQAGFKRYETSAFNRDGLPCRHNVNYWQFGDYIGIGA
                     GAHGKISYHDKIERTVRVRHPKDYLAGIQDGSAIKRERVEKNALPFEFMLNALRLIDG
                     VPSHFYNERTGLNFGTIAHAVKDATARGLLDKNPSRLQTTPLGQRFLNDLLQLFL"
     gene            complement(1856..2494)
                     /gene="rdgB"
                     /locus_tag="PK856_08535"
     CDS             complement(1856..2494)
                     /gene="rdgB"
                     /locus_tag="PK856_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HRL02452.1"
                     /translation="MFSEIVLASNNAGKLREFKAFFEPYGVQVIPQGELNVPECPEPH
                     CTFVENALAKARHASLVTGRPALADDSGICATALNGAPGVHSARYAGEHKNDADNNTK
                     LGLALRDEANKEVWYVCVLVLVRSHDDPQPIILDGVWQGEWQEEARGDNGFGYDPHFY
                     VSQYKQTAAEIPTTLKNRISHRGRAMLGLMQKMDELFHLHGLDVPEKAFFND"
     gene            complement(2484..2726)
                     /locus_tag="PK856_08540"
     CDS             complement(2484..2726)
                     /locus_tag="PK856_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02453.1"
                     /translation="MKTNFVKRALLAVVLFTVLFALLMIAAAYFWTHDSARIAIVCFI
                     AAFISMLCQFGSILLFARHKQRQALMQQQRKNNDVQ"
     gene            complement(2775..>3106)
                     /locus_tag="PK856_08545"
     CDS             complement(2775..>3106)
                     /locus_tag="PK856_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02454.1"
                     /translation="GDSERNWRRVGLALDRVGLTVRQVEASQRIYIVQPTTAESEAVS
                     NEKPGLFKRVFSRKKKETVTARDSLVVKLIPVQGGDRLVILDKQAAPYKGRDLNQYLS
                     RLQTELR"
BASE COUNT          739 a          756 c          833 g          778 t
ORIGIN      
        1 gggcaaaatc caagcatgag catggatttt tgcacaaatt aatggctttt aatgcaagct
       61 caaacagcat ggctgcacat taaaaaccgt gcgtggttat ggtgtggttt ggatggggtt
      121 taagtcaaaa tcggtcagtc aaatgggaag cgtgcgaggt gaatgatggc tgaagatagc
      181 actgagtgat gtactggtaa gtttggattc attcgtcaca accaatttaa tcaccattga
      241 aaaagcggcc tgtaaacatt tacaggccgc atttcattca gcgatgactt attccaaaac
      301 caagatggct tcggcttcta ccaacacgcc tttaggcaag ctgttgatgc ctaccgcagc
      361 gcgagctggg taaggttggc tcatgtagcg ggccatggtt tcgttgaaag tggcgaagtt
      421 ggccaaatcg gttaaatatg cattgatttt aaccacttgg tcaaacgtac cgccagcagc
      481 ttcaatcact gcggccaagt ttttgaacac ttgttcggtt tgttcagcaa aaccaccggc
      541 aaccacgtcg cctgttgctg gatccatagg gatttgacca gaaatccaca cggtattgcc
      601 tgcgcgtacg gcttgtgagt aagcgccaat ggcggcaggg gctttgtctg tgtgaatgat
      661 ttgtttgctc atgctgcttc ctttttgaat ggattataag aacaactgca gtaaatcgtt
      721 taaaaaacgc tgacccaatg gtgtcgtttg caagcggctt gggtttttgt ccaacaagcc
      781 gcgagcggtc gcgtctttga ctgcgtgagc gattgtacca aaattcaggc cagtgcgttc
      841 attatagaag tggctgggca cgccatcgat aaggcgcaag gcattgagca tgaattcaaa
      901 cggcaaagcg tttttttcaa cgcgctcacg cttgatggcc gagccgtctt gtatgcctgc
      961 caaatagtct ttggggtggc gcacgcgcac ggtgcgctcg attttgtcat ggtaggaaat
     1021 cttgccatgt gcacccgcac cgatgccgat gtagtcgcca aactgccaat aattgacatt
     1081 gtggcggcaa ggcaagccat cgcgattgaa tgccgaggtt tcgtaacgct tgaagccggc
     1141 ctgtaacaag gttgcatgca cggcttcttc aatgtcgatc aaggcatcgt cgtcgggcag
     1201 gttttttggt ggtgtgtggc caaacgcggt gttgggttcc agcgtcaatt ggtaggccga
     1261 gatgtgcggt gcacccaagt ctatggcggt ttgcacatcg cgcacggctt gttctatgct
     1321 ttgcttgggc aaggcataca tcaagtccac attgaagcgg ggaaacaact tacaggccgc
     1381 ttctatggct gttattgctt gctggtcatc gtggatgcgg cccaaggctt gcaaatgctc
     1441 aggctgaaag ctttgcacgc ccagcgataa gcggttaacc cctgcagcgg caaagccggc
     1501 aaagcgttcg cgctcgaagg tgccaggatt ggcttccatg gtgatttcgg cattgggcaa
     1561 caggcgcacg cgcgcccgta tgcccgcgag cagtttgtct atggatgcgg cctgaaacag
     1621 gctgggcgtg ccaccaccta tgaatagggt ttccacgctg cggccccaaa tgtgcggcaa
     1681 ctcgtgttcc aaatcttgta acaaggcttg gatgtaagcc tcttctggca agccttcgct
     1741 gctggattgg tgtgagttga aatcacaata cggacatttt tgcacgcacc aaggcaggtg
     1801 cacatacaaa gacaaaggcg gcagggcggt gaggccgctg cctatcgggt gaaactcaat
     1861 cattgaaaaa cgctttttct ggcacgtcta agccatgcaa atgaaacaac tcgtccattt
     1921 tttgcatcaa gcccaacatg gcgcggccac ggtggctgat gcggtttttc aaggtggtgg
     1981 ggatttcggc cgcggtttgc ttgtattggc tcacgtaaaa atgcgggtcg tagccaaagc
     2041 cattgtcacc acgggcttct tcttgccatt cgccttgcca cacgccatcc aaaataatcg
     2101 gctgtgggtc gtcgtgtgag cgcaccaata ccaacacaca cacataccac acttctttat
     2161 tggcttcatc gcgcaaagcc aaacccaatt tggtgttgtt gtcggcatcg tttttgtgtt
     2221 cgcctgcata gcgggcagaa tgcacgccag gtgcgccatt caaagcggtg gcgcaaatgc
     2281 cggaatcatc ggccaaggca gggcggcctg tcaccaagct ggcatggcgc gctttggcca
     2341 aagcattttc cacaaaagtg caatgtggct ctgggcactc aggcacattc aattcgccct
     2401 gggggatgac ttgcacccca taaggctcaa aaaaagcctt gaattcacgc aatttgccgg
     2461 cattgttgct ggccaaaacg atttcactga acatcgttgt tctttctttg ttgttgcatc
     2521 aatgcctgtc tttgtttgtg gcgggcaaag agcaagatgc tgccaaactg gcacagcatg
     2581 gaaataaaag cggcgatgaa acacacgatg gcgatgcggg cgctgtcgtg ggtccaaaaa
     2641 taagctgcgg ctatcattaa gagtgcaaac agcacggtaa acagcacaac ggccaataaa
     2701 gctcgtttga caaaatttgt tttcatagtt aaaaaaaggc agcctgaagc tgccttggta
     2761 aaaaatcctg aaaattaacg caattcggtt tgtaaacggc tcaggtattg gttcaagtca
     2821 cggcctttgt agggagcggc ttgtttgtcc aaaatcacca agcggtcgcc accttggact
     2881 ggaatcaatt tcaccaccaa actgtcgcga gcggttacgg tttctttttt cttgcggctg
     2941 aacacgcgtt tgaacaaacc aggtttctca ttgctcaccg cttcgctttc ggctgtggtc
     3001 ggttgcacaa tgtaaatgcg ttgagacgct tcgacttgtc gcacggtcaa gcccacgcgg
     3061 tctaaggcca agcccacacg gcgccagttg cgctctgaat cgcctt
//
LOCUS       DAOMVK010000260         3103 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_9248_length_3103_cov_3.148950, whole genome shotgun sequence.
ACCESSION   DAOMVK010000260 DAOMVK010000000
VERSION     DAOMVK010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9248_length_3103_cov_3.148950"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PK856_08550"
     CDS             <1..180
                     /locus_tag="PK856_08550"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRL02455.1"
                     /translation="IFERFERVVPTGNKASGLGLGLHIVKEIVVAHQGTIHVESDIGT
                     GSKFTVELPLQATLM"
     gene            complement(292..365)
                     /locus_tag="PK856_08555"
     tRNA            complement(292..365)
                     /locus_tag="PK856_08555"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(328..330),aa:Glu,seq:ttc)
     gene            490..1242
                     /gene="surE"
                     /locus_tag="PK856_08560"
     CDS             490..1242
                     /gene="surE"
                     /locus_tag="PK856_08560"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRL02456.1"
                     /translation="MNILICNDDGYLAQGIRTLAKVTAEFANVRVVAPERDRSAVSNS
                     LTLDRPLSILQAENGFYYVNGTPTDCVHLGLHALPDFVPDMVFSGINHGANMGDDTLY
                     SGTVAAATEAYLLGIPAVAFSLHDKSDQYFGTAEKVVWQVAEYFANNPPKQPLLWNIN
                     IPKVAPDELKGFKVTRLGRRHHMQCIVPAQSPRGKPVYWIGLPGEAADNEAGTDFAAG
                     EEGYVTVTPLSLDLTAYNQMADVGTAIQSWSL"
     gene            1230..1916
                     /locus_tag="PK856_08565"
     CDS             1230..1916
                     /locus_tag="PK856_08565"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HRL02457.1"
                     /translation="MELMMSRSGHWQGFDWRRERMVHRLQHMGIHNPLVLQAMANIPR
                     HVFVEEALRSRAYDETALPIGLAQTISQPYTVAIMTQLLLGTGKTGQEKILEIGTGSG
                     YQTAVLQHIYGNNVYSIERIFELHQLAKKQLRNVGASLVRLVYDDGYAGLPKEAPFDG
                     IIVTAAAPELPLALLQQLAIGGRMVLPLEEANGVQYLWLIEKTPHGFHETCVQEANFV
                     PLINEQDEEF"
     gene            2012..2950
                     /locus_tag="PK856_08570"
     CDS             2012..2950
                     /locus_tag="PK856_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRL02458.1"
                     /translation="MQQSTLLKTGLLTIAIMTVAACNNFKQPAATVENGTSTGTGQVV
                     TSTIPDNNPYGTGPITDNNPYTPPVTSTPSTSTGPYVGNYAPVDINAVFHTVASGDTV
                     YNITKRYGISQDQLRTWNSMPDNNVRLGTRLRVKSPDYVASNSNSNSSYTPPTTTTTS
                     TTPTQTTTKPTQTTSTSTTTTTSTTSTGTRSVGGISWMRPTAGSVITQFNSSNKGIDI
                     AGSAGQPVVAAADGKVVYSGSDLRGYGNLIIVQHNTTFLTAYGHNQSLLVKENDTVKR
                     GQTIAYMGNTHTDRYKLHFELRQNGKPVNPLDYIPN"
BASE COUNT          867 a          839 c          686 g          711 t
ORIGIN      
        1 atctttgaac gctttgagcg ggttgtccct acaggtaata aagcatctgg cttgggcttg
       61 ggtttacaca ttgtgaaaga aatcgtggtc gcccaccaag gcaccattca tgttgaaagc
      121 gacatcggca caggctcaaa attcacagta gagctgccat tgcaagccac actgatgtaa
      181 cgcgatacaa ataataaaag cggcctgtac accagttaca ggccgctttt atattgaatg
      241 ctgtgtcatc taaaccaaac tgtaaacaag tgactcaaca ccacgatgga gcacgcccac
      301 agggattcga accccggttg ccactttgaa gaagtggtgt ccttggccag ctagacgatg
      361 ggcgcgatag ctgagtagtc atggttacag cgcgccaaga taaaccaaag cctatggcct
      421 gtcaaacgcc tttccccttc cgaaaataat taatttcggt acaatagcca ccacagggga
      481 ataaacacaa tgaatatttt aatttgcaat gacgatggtt atttggcaca agggatacgc
      541 actctggcca aagtaacagc cgaatttgcc aatgtgcgcg tggtggcacc agagcgcgat
      601 cgcagcgccg tcagcaactc actcactttg gatcgtcctt tatccattct tcaagccgaa
      661 aatggctttt attatgtgaa tggcacccca accgactgcg tccacctagg cttacatgcc
      721 ctacccgact ttgtcccaga tatggtgttt tcaggcatca accatggtgc aaacatgggc
      781 gacgatacct tgtactctgg cacggttgcc gcagcgactg aagcctactt gcttggcatt
      841 cctgccgtcg cattttcgtt gcatgacaaa tcagaccaat actttggaac tgcggaaaaa
      901 gtggtgtggc aagtggccga atactttgcc aataacccac ccaagcaacc acttctgtgg
      961 aacatcaaca tccccaaagt ggctcctgac gaactaaaag gcttcaaagt cactcgcttg
     1021 ggccgtcgcc atcacatgca gtgcatcgtt ccggcgcaaa gcccgcgtgg caaacctgtg
     1081 tattggatag gcttacctgg tgaagctgct gacaatgagg cgggtaccga ctttgctgcc
     1141 ggcgaggaag gttatgtgac cgtcacacct ctatcactgg atttaaccgc ttacaatcaa
     1201 atggccgatg tcggcaccgc catccaatca tggagcttat gatgtcgcgc agcggccatt
     1261 ggcaaggttt tgattggcga cgcgagcgca tggtacacag actccaacac atgggcatac
     1321 acaacccctt ggtgttacaa gccatggcca acattccccg ccatgtgttt gttgaagaag
     1381 ccttgcgctc tcgggcttac gatgaaaccg cacttcccat cggtttggca caaaccattt
     1441 ctcaacctta tactgtcgcc atcatgacgc aactgctctt aggcactggc aaaacaggtc
     1501 aagagaagat tttggaaatt ggcactggca gcggttacca aaccgccgtt ttacagcaca
     1561 tctatgggaa caatgtatac tctattgagc gcatattcga attgcatcaa ttggccaaaa
     1621 agcaattgcg caatgtaggc gccagtttgg tgcgtttggt ttatgacgac ggctatgcag
     1681 gcttacctaa agaagcaccc tttgacggca tcattgtcac tgcagccgcg cctgaattac
     1741 ccttggcttt gcttcaacaa ttagccatag gcggacgcat ggtattgcct cttgaagagg
     1801 ccaatggggt gcaatatctg tggctcattg aaaaaacccc acacggtttt catgaaacct
     1861 gcgtacaaga agccaatttt gtaccgctca ttaacgagca agacgaagaa ttttaaacca
     1921 tgtaaaatag ccgctgctcc aatgctcgcg gctgtgattt ggcattatac tgccactgaa
     1981 tacgattcca actaacaaag gttatgcaca gatgcaacaa tcgacgctat tgaaaactgg
     2041 tttgttgacc atcgccatca tgactgtggc ggcctgtaac aacttcaaac aacccgctgc
     2101 caccgtagaa aatggcacca gcacaggcac tggacaagtg gtaaccagca ccattcccga
     2161 taacaatcct tatggcactg gcccgattac cgacaacaat ccctataccc cacccgtaac
     2221 aagcacccca agcacaagca cagggccgta tgtgggcaat tatgcgcctg tcgacatcaa
     2281 cgctgtgttc cacacagttg cttctggcga cacggtttat aacatcacca aacgctatgg
     2341 catcagtcaa gaccaattgc gcacttggaa cagcatgcct gacaacaacg tacgcttggg
     2401 cacacgcttg cgtgtgaaat cacccgatta tgtagcgagc aacagcaata gcaactcaag
     2461 ctatacccca cctactacga ccacgacaag caccacacca acccaaacca ccactaaacc
     2521 gacacaaacc accagcacct caaccacgac cacaacttct actacgagca caggcacacg
     2581 ctcagtgggt ggcatttcat ggatgcgtcc aacagctggc agcgtgatta cccaattcaa
     2641 cagctccaat aaaggcattg atattgctgg tagcgcaggt caacctgtag ttgctgcagc
     2701 cgatggcaaa gtggtttaca gtggcagtga tttgcgtggt tatggcaatt tgattattgt
     2761 gcaacacaac accaccttct taaccgctta cggccacaac caaagcctgt tggtgaaaga
     2821 aaatgacacc gtaaaacgcg gtcaaaccat tgcctacatg ggcaataccc ataccgaccg
     2881 ctacaaactg cattttgaat tgcgtcaaaa tggcaaaccg gtcaacccat tggattacat
     2941 tcctaactaa gcagtaagcg gcctgtaaga cttacaggcc gcttgttgaa cccaatatcg
     3001 cccacgtgcg atatttttat ttgaggcaat tatgtggttt aaacaagtca gctttttcgt
     3061 actcccccaa gacgccgaat tgaatttaga agacatccaa acc
//
LOCUS       DAOMVK010000261         3086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_9321_length_3086_cov_2.689871, whole genome shotgun sequence.
ACCESSION   DAOMVK010000261 DAOMVK010000000
VERSION     DAOMVK010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9321_length_3086_cov_2.689871"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PK856_08575"
     CDS             <1..443
                     /locus_tag="PK856_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02459.1"
                     /translation="DIKSDIILLDQAVTQSGMNDSAIQDLQKEVAAAKSPEEFTQAAA
                     KIRDQYQSYHQAINALDFKTDEVRGIRDELSTQSGDLISSLDEFVNLNIRSNVSYTPV
                     QADRVHVTMTRVENKIETSEKNTLQTLKKLQALGKDNGVDLQIQ"
     gene            615..2921
                     /locus_tag="PK856_08580"
     CDS             615..2921
                     /locus_tag="PK856_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HRL02460.1"
                     /translation="MNTHPSAQKPIFSYKKYWAKRFGTAPFLPTTRAEMDELGWDSCD
                     IIIVTGDAYIDHPSFGMALVGRLYEAQGYRVGIIAQPDWTSVDAFRELGEPNLFFGVT
                     SGNMDSMINRYTADKKPRSDDAYTPDAALNKRPDRAVVVYSQRCKEAYPGVDVMIGSI
                     EASLRRIAQYDFWSDKVRPSILINARADILLYGSGERALIEVAERRAKGIRLKDIRDV
                     RGTAFLIQHGKTPDNQEWTEIDSTTVDVPGKVDTLLSPYMEYSPEELAARDEEVKEHA
                     KPVLFMTAKERAEARRQARHQQVVRIPSFEAVAYDPVLYAHASRTLHLESNPGNARAL
                     VQMHGERDVWLNPPPIPLTTEEMDYVFGLPYARNPHPSYGDAHIPAWEMIRFSINIMR
                     GCFGGCTFCSITEHEGRIIQSRSHESVIKEIEEIRDKTNDFKGYISDLGGPTANMYRM
                     ACKSHDIEKNCRKLSCVWPDICPNLNTDHSSLIELYRKARSVEGVKKISIGSGLRYDL
                     AVESPEYIKELVTHHVGGYLKIAPEHTEAGPLSMMMKPEMKKYDRFKELFEKASKEAG
                     KEQYLIPYFIAAHPGTSDEDMMNLALWLKKNNFRLDQVQTFMPTPMALATTMWHTGRN
                     PLKKLSRSSTKVDIVKEGKRRKLHKAFLRYHHPDNWPMLRNALHDMGRDDLIGYGKKH
                     LIPPTQPADWVPPVKGASKAKGKQGQAAATNSAANPSRQTTKGKFQTARTAWHPTERF
                     QDAGKGGNKAKTGGAGNTAAKRSKPKAR"
BASE COUNT          868 a          875 c          719 g          624 t
ORIGIN      
        1 acgacatcaa atccgacatc attttgctcg accaagccgt tacccaatcg ggcatgaacg
       61 acagcgccat tcaagacttg caaaaagaag tggctgctgc caaatcgcct gaggaattca
      121 cccaagcggc cgccaaaatt cgcgaccaat accaatccta ccaccaagcc atcaacgccc
      181 tcgatttcaa aaccgacgaa gtccgtggca tacgcgacga attgagcaca caaagcggcg
      241 acttaatcag cagtttggat gaatttgtca atctcaacat ccgctccaat gtgtcttaca
      301 cgcctgtaca agccgatcgc gtgcatgtca ccatgacacg ggtggaaaac aaaatcgaaa
      361 cctcggaaaa aaacaccctc caaaccctga aaaaattaca ggctttgggc aaagataatg
      421 gcgtggactt acaaattcaa taagcccaaa tcaaaaaaag cagccatgca aacagcggcc
      481 tgtaagcttt acaggccgca tatttgaaaa ataaccttta ttttcaatct tttcactgcc
      541 atccctgccc ctcaaggcca atttgtgaga aaatgctgca cttataaaat agctcggcaa
      601 gatctcataa cagcatgaac acacatcctt cggcgcaaaa gccgattttt tcgtataaga
      661 aatactgggc caagcgcttt ggtactgcgc cctttctccc cactacccgc gccgaaatgg
      721 acgaattggg ttgggattct tgcgacatca tcatcgtcac tggcgacgcg tacatcgacc
      781 accccagctt tggcatggcc ttggttggcc gcttgtacga agcacaaggc taccgcgtgg
      841 gcatcattgc ccaacccgat tggaccagtg tggatgcctt ccgcgaattg ggtgagccca
      901 atctgttttt tggtgtgact tcgggcaata tggactcgat gatcaaccgc tataccgccg
      961 acaaaaaacc gcgttcagac gatgcctata ccccagacgc tgcgctgaat aagcgccctg
     1021 accgtgcggt ggtggtgtat tcacagcgct gtaaagaggc ttatcctggt gtagacgtga
     1081 tgataggctc gatagaagcg agtttgcgcc gcattgccca atacgatttt tggtcggaca
     1141 aagtgcgtcc atccattctc atcaatgccc gcgccgacat cttgttatac ggcagtggcg
     1201 aacgcgcttt gattgaagtg gccgagcgcc gcgccaaagg catacgcctc aaagacatcc
     1261 gcgatgtgcg cggtacggca tttttaatcc aacacggcaa aaccccagac aaccaagagt
     1321 ggacagaaat cgattccacc acggtagacg tgccaggcaa agtggatacc ttgctcagcc
     1381 cttacatgga atacagccca gaagaattgg ctgcccgtga cgaagaggtg aaagaacacg
     1441 ccaaacccgt gttgttcatg accgcaaaag agcgcgccga agcccgtcgc caagcgcgtc
     1501 accaacaagt ggtgcgcatc ccaagctttg aagccgtggc ttacgaccct gtgctctatg
     1561 cccatgccag ccgcactttg cacttggaat ccaaccctgg caatgcccgc gccttggtgc
     1621 aaatgcacgg tgagcgcgat gtgtggctca acccgccacc gattcccctc accaccgaag
     1681 aaatggacta tgtgtttggc ttgccctacg cgcgcaatcc acacccgagc tacggcgatg
     1741 cccacattcc cgcttgggaa atgattcgtt tctccatcaa catcatgcgc ggttgttttg
     1801 gtggctgtac cttctgctca attaccgaac acgaaggccg catcatccaa agccgttcgc
     1861 acgaatcggt gattaaggaa atcgaagaaa tccgcgacaa aaccaacgat ttcaaaggct
     1921 atatttccga tttgggcggc cctactgcca atatgtaccg catggcgtgt aaatcacacg
     1981 acatcgagaa aaactgccgc aaactctctt gtgtgtggcc agacatttgt ccgaacctga
     2041 acaccgacca ctcgtctttg attgagttat accgcaaagc gcgttcggta gaaggcgtga
     2101 agaaaatcag cataggctca ggcttgcgct acgatttggc agtggaaagc cctgaataca
     2161 tcaaagaatt ggtgacccac cacgtgggcg gctatttgaa aatcgccccc gagcacactg
     2221 aagctggccc gttgtcaatg atgatgaagc ctgaaatgaa aaaatacgac cgcttcaaag
     2281 aattgtttga aaaagccagc aaagaagcgg gcaaagaaca gtatttgatt ccgtatttca
     2341 tcgcggccca cccaggcact tcagacgaag acatgatgaa tttggcgctg tggctgaaga
     2401 aaaacaactt ccgcctcgac caagtgcaaa ccttcatgcc aacgcccatg gctttggcca
     2461 ccaccatgtg gcacacaggc cgcaatccgc tgaaaaaact gtcgcgcagc tctaccaaag
     2521 tcgacatcgt taaagaaggc aagcgccgca aattgcacaa agcctttttg cgctaccacc
     2581 atccagacaa ctggccgatg ctgcgcaatg ccttacacga catggggcgt gacgatttga
     2641 tcggctatgg caaaaagcat ttgataccgc caacccagcc tgccgattgg gtgccacctg
     2701 tcaaaggtgc ttccaaagcc aagggcaaac aaggccaagc agcggcaacc aacagcgctg
     2761 ccaaccctag ccgccaaacc accaagggca aattccaaac cgcccgcacg gcttggcacc
     2821 ctacagagcg tttccaagac gctggcaaag gtgggaataa agccaaaaca ggcggcgcag
     2881 gaaataccgc agccaagcgc agcaagccca aagcacgtta aatcaagttt gccagcacag
     2941 atttacccga taccacagct caggctgtgg tattttttat gctattgtta agaccgcacc
     3001 catttacacc caaggatacg gtatgagcaa cacacaatgg tatatcggca gtgaaaaagg
     3061 catagaacac atcttaactc ggcctg
//
LOCUS       DAOMVK010000262         3086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_9322_length_3086_cov_2.462224, whole genome shotgun sequence.
ACCESSION   DAOMVK010000262 DAOMVK010000000
VERSION     DAOMVK010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9322_length_3086_cov_2.462224"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /gene="serS"
                     /locus_tag="PK856_08585"
     CDS             complement(<1..931)
                     /gene="serS"
                     /locus_tag="PK856_08585"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRL02461.1"
                     /translation="MLDIQVLRNEMQRVTQAMQSRNFAFDAAQFSQLDEERKTLQTKT
                     QELQAKRNQVSKQIGIAKSKGEDASSIMAEVGNLGEELKQVEDAYKVVQEQLDALLLH
                     LPNLPSADVPVGRDENDNVEVRKVGTPRAFDFDVKDHVDLGAPLGLDFETGAKLSGAR
                     FTVMRGQIALLHRALAQFMLNTHTLKHAYTEHYTPYIVNPEILFGTGQLPKFAEDMYA
                     VQCGGEEATQSQYLISTAEITLTNTVRESILKADELPIKMTAHSPCFRSEAGSYGKDT
                     RGLIRQHQFDKVEMVQVVHPEKSYQALEEMVGHA"
     gene            1107..2483
                     /locus_tag="PK856_08590"
     CDS             1107..2483
                     /locus_tag="PK856_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02462.1"
                     /translation="MQPNNNLVFYLAGDNDEPSKVSGEQNSIVEPVSSSDVLPESDVS
                     SEASEQSLLSEHAPQIEELNPVDLDEVDWIATLENIRHQRRGDDVTNEDTAFANAAGQ
                     ANVLKVTSMKTTQLHQPTASALAAGKDLEKLSYSLKEAQRLVKNTPATEYSAPKATEA
                     PVWSMEDLEKQIQSLSGNHTHEDIEAVSFETEPFIVDERTDNEFEAVLSEDENNQTEL
                     NALLDDLKAAAKQVPFDEYAVESTQLHSAPSQELIDKANEVAEKTALSLEERHKILDG
                     FLSLWQHKREPELNENAERKVMFKEEWHAHNSDVLQAASESLETPEHVMWLKTLPEAM
                     MDGEVNTDVTSVSDEVAVTLEQPEEATQQNMYAPVHVSIVTPELNANERLSVVSEQEL
                     LAQLAIKLKPYFVDMLAGLVRNELNRQTTALVQRLQDNINEEIPMLVDDVLQENLQNV
                     LREIKQSQ"
     gene            2562..>3086
                     /gene="phoU"
                     /locus_tag="PK856_08595"
     CDS             2562..>3086
                     /gene="phoU"
                     /locus_tag="PK856_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HRL02463.1"
                     /translation="MAEHISSQFNQELETVRTMVMKMGGLVEQQLQNVIKGLEGNNSG
                     LLQEVVAADRTVNEMEVSIDDQCQLIIARRQPAAGDLRLVLTVTRIITDLERIGDEVK
                     KIALYGNGLISKNGVLPHQFHDLNRLAQRSLGMIHTALDAFARLDATVVVQLNKEDDL
                     LDYEYDNLLRQQITY"
BASE COUNT          866 a          654 c          759 g          807 t
ORIGIN      
        1 cggcatgacc aaccatttct tctagggctt ggtaagattt ttcaggatgg accacttgca
       61 ccatttcaac tttgtcaaat tggtgttggc gaataagacc gcgggtgtct ttgccgtaag
      121 aacctgcttc tgagcggaag caaggagaat gtgccgtcat tttaatgggc aattcatcgg
      181 cttttaaaat gctttcacgc acagtgttgg ttaaggtgat ttcggctgta gaaatcaaat
      241 actggctttg tgtcgcttcc tcaccgccac attgtacggc atacatgtct tcggcaaact
      301 tgggcaactg acctgtacca aacagaatct caggattgac gatgtaaggg gtgtaatgct
      361 cagtataagc atgtttgagg gtgtgagtat tgagcatgaa ctgcgccaac gcacggtgca
      421 ataatgcgat ttggccacgc atcacggtaa aacgagcacc cgataatttg gcgccagtct
      481 caaaatccaa gcctaatggc gcacccaaat caacatggtc tttgacatca aaatcaaacg
      541 cacgaggtgt gccaacttta cgcacttcca cgttatcgtt ctcatcgcga ccaacaggaa
      601 catcagcact gggtaagttg ggtaagtgca acaataaagc atccaattgc tcttgcacca
      661 ctttgtaagc atcctcaact tgtttcaatt cttcgcccaa gttacccact tcagccatga
      721 tgctgcttgc atcttcacct ttgcttttgg cgatgccaat ttgcttggat acttggttgc
      781 gcttggcttg caattcttgg gttttggtct gcaaggtttt gcgttcttcg tccaattggc
      841 taaattgtgc tgcatcaaac gcgaaatttc ggctttgcat ggcttgggtg acgcgctgca
      901 tctcattgcg caacacttga atatctaaca tggttttgac ccatacaata aaaacaaaca
      961 ttgtagcggt ctgtgtgcca tttgtcaggt aggctctgcg gataaattcg cgtgtaactg
     1021 gctgcatcgc agcgatagaa tgatgctaga atgattccat taaattaaat gactgtatta
     1081 ctgcatacca aaggataaca agaaccatgc aaccgaataa taatttggtc ttttatttag
     1141 ctggtgacaa cgatgagccc agcaaagtgt ctggtgagca aaattcaatt gttgaacctg
     1201 tttcttcaag cgacgtgtta ccagaatcgg atgtttcaag cgaggcttca gagcagtcat
     1261 tgctttctga acatgcacca cagattgagg aattaaatcc cgttgacttg gatgaagtgg
     1321 attggattgc aacattagag aacatacgcc accagcgccg tggggacgat gtgacgaatg
     1381 aagacacagc atttgccaat gcagcgggcc aagccaatgt gctcaaagtc acctcaatga
     1441 aaaccaccca gttgcatcaa ccaacagctt cggcattggc tgcgggtaag gatttggaaa
     1501 aactgagcta cagcctgaag gaagctcaac gtttggtaaa aaacacccca gcgacagaat
     1561 attctgctcc caaggcaacg gaagcgcccg tttggagcat ggaggatttg gaaaaacaaa
     1621 ttcagtcctt gagtgggaac catacacacg aagacattga agcagtttca tttgaaactg
     1681 agccttttat tgtggatgaa cgcacagata atgagtttga ggctgtgtta tccgaggacg
     1741 aaaacaatca gaccgagctt aatgctttgc ttgatgactt gaaagctgcg gcaaaacagg
     1801 ttccatttga tgagtatgct gttgaatcga ctcaattgca ttcggcgccg agtcaagagt
     1861 tgatagacaa ggccaatgaa gtggctgaaa aaacggcttt gagtttggaa gagcgacata
     1921 agattttgga tggcttcttg tcattgtggc aacacaaacg tgaacctgaa ttaaatgaaa
     1981 atgccgaacg caaagtcatg ttcaaagaag agtggcatgc ccataacagc gatgttttac
     2041 aggccgcatc agaatcactt gagacacctg agcatgtgat gtggttaaaa actctgcctg
     2101 aagcaatgat ggatggcgag gtcaacactg atgtgacatc agtctctgat gaagtcgctg
     2161 taacgctaga gcagccagaa gaggcaaccc aacaaaacat gtatgcccct gtgcatgtca
     2221 gcattgtgac acctgagttg aatgccaatg agcgtctcag tgtggtcagc gagcaggagt
     2281 tattggcaca attggccatt aagttgaagc cttactttgt ggacatgttg gcaggcttgg
     2341 tgcgcaatga actcaatcgt caaaccactg cgttggtgca gcgtttgcaa gacaacatca
     2401 atgaagaaat tccgatgttg gtagacgatg tgttacagga aaacttgcag aatgtgttgc
     2461 gtgaaataaa acagtcacaa tagcccatgt caattgtcac atgaatgtca tgatagattg
     2521 ttatgcttgt ggcctatgat tgaaacgagg atatcttgat aatggctgaa cacatttctt
     2581 cacagtttaa ccaagagctg gaaaccgtcc gcaccatggt aatgaaaatg ggcggtttgg
     2641 ttgaacagca attacaaaac gtcattaaag gcttggaagg caacaacagt ggtttgctgc
     2701 aagaagtggt ggctgccgac cgcactgtga atgagatgga ggtgagcatt gatgaccagt
     2761 gtcaattgat catcgcccgc cgtcagcctg ctgcaggcga tttgcgcttg gtgttgactg
     2821 ttacccgcat catcaccgat ttggagcgca tcggcgatga ggtcaaaaag attgctttgt
     2881 atggcaatgg tcttatcagc aaaaatggcg tgttgccaca ccaatttcat gatttaaacc
     2941 gtttggcaca gcgctctttg ggcatgatac acacggcttt ggatgcgttt gcgcgtttgg
     3001 atgccacggt ggtggtgcag ttgaacaaag aagacgattt gttggattac gaatacgaca
     3061 atctgttgcg tcagcaaatc acttac
//
LOCUS       DAOMVK010000263         3082 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_9341_length_3082_cov_2.747275, whole genome shotgun sequence.
ACCESSION   DAOMVK010000263 DAOMVK010000000
VERSION     DAOMVK010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9341_length_3082_cov_2.747275"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1705
                     /locus_tag="PK856_08600"
     CDS             <1..1705
                     /locus_tag="PK856_08600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HRL02464.1"
                     /translation="TTPNGFFVWLPEAYGVESVEVIKGANSNLYGASGAGGVVNLVSK
                     RPKSEPAGEVKIGLGTDKRGSIAADYSGVLTEDNSVRYRVVAQARREDSQIEFANMKH
                     YYFAPSLTWDISDNTNLTLLASYQHEKGRPTNGFLPAYGTIIDTPYGKINRDTSMGEP
                     DFDKTDRTQVSAGYEFQHDFGNGWKFSQNYRYSHMDLLNQGVFAWGSDGKRTAYRGYS
                     YSDGDQNLHNLDNRVSKLWQGERFSNTLLVGLDYLHSTTDGKNNGFGYVPGLDIFNPQ
                     YGADITLTGDPYHIKLKQTGLYAQNQFKWDNWLLNAGVRHDKAKSSGYIGTVDQSYDV
                     NHTSYNAGLMYQFDNGLSPYVNYSESFRPISGGDAQNRAYKPYEGQQFEAGLKYSPVD
                     LNGRFSIAYFDLKEKNALVSDASNVQVQAGERRGRGVELQANVNLNDNWSLAGAYTYT
                     DSKQDLSTNTTIRSPMMPRHMASTQVAYQFNAGALNGLKLGTSLRYVGSTTDDQYYAG
                     HTIPSYTLWDAMAQYTFAKDWQVQLNARNLTDKDYVSACSFYCYYGAGRSVEASLSYK
                     W"
     gene            complement(1780..2979)
                     /locus_tag="PK856_08605"
     CDS             complement(1780..2979)
                     /locus_tag="PK856_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HRL02465.1"
                     /translation="MAISIIAMMVLAACNQGGGAAAGGDSKAASGESAQTSSGGGDGK
                     EIIIGFSAPLTGPQSHYGEDYKSGVQMAIDEANEQGITINGGPAKFKLDAQDDGADPK
                     QATQVAERFVDLKVAGVIGHFNSGTTIPASKVYEENGIPNISMATSPVLTQQGFKNVF
                     RSMTSDSQQGLVLGKYAVEKLGYKTVGIIDDRTAYGQGLADEFEKSAKAAGATILPRQ
                     FTNDKATEFQSILTAFKESPPDVIFYGGADPQSAPMVVQMKRLGIKSQFLSGEMTKNP
                     NFIKVAGESAEGVMASLAGVPLAKLPKGPEFETKYKAKFGKEVQIYAPYGYDGTWAMI
                     NAMKAANSSKPADYLPKLAATDMPGITSDHFAYDDKGDLKDVAITMYKVEKGQWVPVE
                     SLTSSSK"
BASE COUNT          785 a          780 c          727 g          790 t
ORIGIN      
        1 caccaccccc aatggctttt ttgtgtggct acctgaagcc tacggcgtgg aaagtgtgga
       61 agtcatcaaa ggcgccaatt ccaatttgta tggcgcatct ggcgcaggcg gcgtggtgaa
      121 tttggtgagc aaacgcccca aatctgagcc tgctggcgaa gtgaaaatcg gcttgggtac
      181 agacaagcgc ggcagcattg ccgccgatta cagtggcgta ttgaccgaag acaacagcgt
      241 gcgctaccgc gtggtggcac aagcgcgccg tgaagacagc caaatcgaat ttgccaacat
      301 gaagcattac tattttgccc cgagcctgac ttgggacatt tccgacaaca ccaacctgac
      361 cttgctcgcc agctaccaac acgaaaaagg ccgccccacc aatggctttt tgccagctta
      421 cggcaccatc atcgataccc cttacggcaa aatcaaccgc gatacctcca tgggtgagcc
      481 tgattttgac aaaaccgacc gtacccaagt gagcgcgggc tatgaattcc aacacgattt
      541 tggcaatggt tggaaattca gccaaaacta ccgctacagc cacatggatt tgctcaacca
      601 aggcgtgttt gcttggggct cagatggcaa gcgcactgct taccgcggct attcttacag
      661 cgatggcgac caaaacctgc acaaccttga caaccgtgtg agcaaattgt ggcaaggcga
      721 gcgctttagc aataccttat tggtgggctt ggactatttg cattccacca ccgatggcaa
      781 aaacaatggc tttggctatg tgccaggctt ggacatattc aacccgcaat acggtgctga
      841 catcaccttg acgggcgacc cttaccacat caaattgaaa caaacaggtc tttatgccca
      901 aaaccaattc aaatgggaca attggttgct gaatgcgggt gtgcgccatg ataaggcaaa
      961 aagctcaggc tatatcggca cagtcgacca aagctacgat gtcaaccaca cctcttacaa
     1021 cgcaggcttg atgtaccaat ttgacaatgg tttgtcaccg tatgtgaatt actctgaatc
     1081 tttccgcccg atttcaggtg gtgatgccca aaaccgtgcc tacaaaccgt atgaaggtca
     1141 acagtttgaa gccggtttga aatacagccc tgtggatttg aatggccgtt tctccatcgc
     1201 ttatttcgat ttgaaagaga aaaatgcgct ggtgagcgat gcgtctaatg tgcaagttca
     1261 agcaggtgaa cgccgtggtc gtggggtgga gttacaggcc aatgtgaatt tgaatgacaa
     1321 ttggtctttg gcaggcgctt acacctatac cgattccaaa caagacttgt ctaccaacac
     1381 caccatacgc tcaccaatga tgccacgcca catggcctca acccaagtgg catatcaatt
     1441 caacgcaggc gcattgaatg gtttgaaact gggcacgtct ttgcgctatg tgggctcaac
     1501 caccgatgac caatactacg caggccacac catccctagc tacactttgt gggatgcgat
     1561 ggcgcaatac actttcgcca aagactggca agtgcaactg aacgcgcgca atttaaccga
     1621 caaagactat gtcagcgctt gcagctttta ttgctattac ggcgcaggcc gcagtgtgga
     1681 agccagcttg agttacaaat ggtaagtctg tattgatgca gtaacataaa aaagcggcct
     1741 gtaaacattt acaggccgct tcttactgat ttttaaaact tatttagaac tagaagtcaa
     1801 actttcaact ggaacccatt ggcctttttc gactttgtac atggtgatgg caacgtcttt
     1861 caagtcacct ttgtcatcgt aggcaaagtg gtcactggtg ataccaggca tgtcggttgc
     1921 agccaattta ggcaagtaat ccgcaggttt ggatgagttt gccgctttca tggcattgat
     1981 catcgcccaa gtgccgtcat agccgtaagg tgcgtaaatt tgaacctctt tgccgaattt
     2041 ggctttgtat ttggtttcaa attcagggcc tttaggcaat tttgccaaag gcacgcccgc
     2101 caaagaagcc atcacgcctt cagcgctttc acccgccact ttgatgaagt tagggttttt
     2161 ggtcatctca ccagacaaga attgagattt gatgcccaag cgcttcattt gcacaaccat
     2221 aggcgcagat tgtgggtcag caccgccgta gaaaatcacg tctggtgggc tttctttgaa
     2281 cgcggtcaag atagattgga attcagtcgc tttgtcattg gtaaattgac gtggcaaaat
     2341 ggttgcgcca gcggctttgg ctgatttctc aaattcgtca gccaaacctt gaccataagc
     2401 tgtacggtca tcaatgatgc cgacggtttt atagcccaat ttttccactg cgtatttgcc
     2461 caataccaaa ccttgttgtg agtcagaggt catggaacgg aatacgtttt taaagccttg
     2521 ctgtgtcaac acagggctgg tggccataga aatgttagga atgccgtttt cttcgtaaac
     2581 tttagacgct ggaatggtgg taccagagtt gaagtgacca atcacacccg ccacttttaa
     2641 gtcgacaaaa cgctcagcca cttgggtggc ttgcttagga tccgcgccat cgtcttgcgc
     2701 atccaatttg aacttggcag ggccaccgtt gatggtgata ccttgctcat tggcctcatc
     2761 aattgccatt tgcacgccgc tcttatagtc ttcaccataa tgcgattgtg ggccagtcaa
     2821 tggtgcagag aaaccaatga tgatttcttt gccatcgccg ccaccgctgc tggtttgcgc
     2881 gctttcgcca gacgctgctt tgctgtctcc accagctgct gcaccgccgc cttgattaca
     2941 ggccgccaaa accatcatag caataatgct gatcgccata tttctcataa ttcttccttt
     3001 gttgttgttt ggtgataatc acttgtgtta cagacttggg taatacattt tgaaaaccaa
     3061 caaagccagc cgcctgattt tt
//
LOCUS       DAOMVK010000264         3079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_9359_length_3079_cov_2.936177, whole genome shotgun sequence.
ACCESSION   DAOMVK010000264 DAOMVK010000000
VERSION     DAOMVK010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9359_length_3079_cov_2.936177"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /gene="lpxB"
                     /locus_tag="PK856_08610"
     CDS             complement(<1..802)
                     /gene="lpxB"
                     /locus_tag="PK856_08610"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HRL02466.1"
                     /translation="MSAQRPLTIALIAGEASGDLLGAHLIAALKQHYPNCRFVGIAGP
                     LMQKEGCESLYPQEALAVRGFVEVIKRLPSILKIRKGVVSQLKAMRPDVFIGIDAPDF
                     NLGVAERLKAAGIPTIHYVSPSVWAWRRERVNKIVHQVNRVLCLFPMEPQLYRDAGGQ
                     ADFVGHPLAQTLPMVPDKTGARAQLKLEEAEQVITVMPGSRVSEIDYMGAVFLDTIPL
                     ILERYPNAQFLIPAATTATRQRLLELLEKDAYKKLPVRVMSAHATLACV"
     gene            988..1719
                     /locus_tag="PK856_08615"
     CDS             988..1719
                     /locus_tag="PK856_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009115337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRL02467.1"
                     /translation="MKTSLLCLGIASALALSACAQTANQTQSANGFRYSEAEMQDLAS
                     ANKLSHRVFYAKPSTGENAAWFDAVKRGDLATVKKMVAAGQNIEAKDSGSLDQTALGW
                     AAFIGYEDMVDFLIAQGASLQATDKGDVYNVLKSAVLGKNVKVVKKIHGLMPKVDLND
                     QSLESDGETLVMVAASNNRIDIVKYLIAQGANVNLSTTTQDKNMGSYDQSPLTYACKR
                     GHVQMQQLLIKHGAVNHRTGKPACD"
     gene            1822..2247
                     /locus_tag="PK856_08620"
     CDS             1822..2247
                     /locus_tag="PK856_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRL02468.1"
                     /translation="MNHLNHLNTLPTAGLIIVHNQALLLAYSNNKQAWYLPGGKIDAG
                     ESAKQALLREIREELALDLETEQLHFYTHISAPAYGETPAVMMAQDCFRCELGDAIPQ
                     AQAEIGAVRYFTLAEFQQLDIQVAGVVQVYARLQADGLV"
     gene            complement(2315..>3079)
                     /locus_tag="PK856_08625"
     CDS             complement(2315..>3079)
                     /locus_tag="PK856_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007341978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HRL02469.1"
                     /translation="GDAVYYAGVVNRAGCNAEYQAVDARIIAKKPQSLDFAQAAALPL
                     TSLTAYEGLFDRLQVNERSDMRNRVLLVIGGSGGVGSQIIQLAKQLSDLTVVATASRP
                     ESQAWCEELGADFVINHRHDLAQEWQKTGLPAPSWVFSTQHTDDYAEQLAALMAPQGK
                     LVLIDDPKALPIGVFKSKSISIHWEFMFTRGMFVTADIARQHDILAEVAALIDAGKIK
                     TTLNKVISPINVANLKQAHSDIESGNSIGKIVLQGW"
BASE COUNT          766 a          845 c          759 g          709 t
ORIGIN      
        1 ccacgcaggc caaagtcgcg tgtgccgaca tcacacgcac aggcagtttt ttatacgcat
       61 ctttttccaa caattccaat aagcgctggc gcgtggctgt ggtggcggcg ggtatcaaaa
      121 attgtgcgtt gggatagcgc tccaatatca atggaatggt gtcgagaaac acagcgccca
      181 tgtaatcgat ttcgctgacg cggctgccag gcatcacggt gatgacttgt tctgcctctt
      241 ccaatttcaa ctgtgcgcgc gcgcctgttt tatcgggcac catgggcaag gtttgcgcca
      301 aaggatggcc aacaaaatcg gcttgtccgc ccgcatcgcg gtacaactgc ggctccatcg
      361 gaaacaggca cagcacacga ttgacttggt gaacgatttt gttcacacgc tcgcgtcgcc
      421 atgcccaaac cgatgggctg acataatgta tcgtcggtat gcctgccgcc ttgagccttt
      481 cggccacgcc caaattgaaa tcgggcgcat caatgccgat gaacacatca ggccgcatcg
      541 ccttgagctg tgacaccacg cctttgcgga ttttcaaaat gctcggcaag cgcttaatca
      601 cttccacaaa cccgcgcacc gccaaggctt cttgtggata cagactttcg cagccctctt
      661 tttgcatcaa cggccctgca atgcccacaa agcgacagtt tgggtaatgc tgttttaaag
      721 ccgcaatcaa atgtgcacca agcaaatcgc ccgatgcttc acccgcgatt aaagcgatgg
      781 taagaggtcg ttgagcactc atgtagccag acattcggaa ataatccgcc attttaactg
      841 aaatttactt cagaggtttt gcttgggctt aaattcgccg ctatggctcc aaacaccaca
      901 cccaacacaa cctgcgtcac acacgctcaa cagctgtgtg gtattgttgt aaaattccgt
      961 tttccccact tagattgaga acacgccatg aaaaccagct tactttgttt gggcatcgcc
     1021 agcgccttgg ctttgagcgc ttgcgcccaa accgcaaacc aaacccaatc tgccaacggt
     1081 ttccgctaca gcgaagccga aatgcaagac cttgccagcg cgaacaaatt gtcccaccgc
     1141 gtgttctacg ccaagccttc taccggtgaa aatgccgctt ggttcgatgc cgtcaaacgc
     1201 ggcgacttgg ccactgtcaa aaaaatggtg gcagcaggcc aaaacattga agccaaagac
     1261 agcggcagct tagaccaaac cgctctgggc tgggccgcgt tcatcggcta cgaagacatg
     1321 gtggactttt tgattgccca aggtgcgagc ctacaagcca ccgacaaggg cgatgtgtac
     1381 aacgtgctca aatctgccgt attgggcaaa aacgtcaagg tggtgaaaaa aatccacggc
     1441 ctcatgccca aagtggattt gaacgaccaa agcttggaat ccgatggcga aaccttggtg
     1501 atggtggcgg caagcaataa ccgcatcgac atcgtcaaat acctgattgc ccaaggtgca
     1561 aacgtgaatt tgtccaccac cacccaagac aaaaatatgg gctcatacga ccaaagccca
     1621 ctcacatacg cctgcaaacg cggtcatgta caaatgcaac aattgctgat taagcatggc
     1681 gcggtaaacc accgcacagg caaacctgct tgcgattgat ggcattaata taaaaagcgg
     1741 cctgtaaatg tacaggccgc ttttttgtgg cacgggtaca tagcccttat aatcagccac
     1801 ccattcccca cgcacagacc catgaatcac ttaaatcacc tcaacacgct gcccaccgct
     1861 ggcctcatca ttgttcataa ccaggctttg ctcttggctt acagcaataa caagcaggct
     1921 tggtatttgc ctggtggcaa aatcgatgcg ggagaaagcg caaaacaggc tttgctgcgt
     1981 gagatacgtg aagagttggc gctggatttg gagaccgaac aattgcattt ttacacccac
     2041 atcagcgcgc ctgcttatgg tgaaacgcct gcggtaatga tggcacaaga ctgttttcgc
     2101 tgtgaattgg gcgatgccat tccgcaagca caggccgaaa tcggcgcggt gcgctatttt
     2161 acgctggccg agtttcagca actggacatc caagtggcag gcgtggtgca agtgtatgcg
     2221 cgtttacaag ctgatggttt ggtgtaagac agtggttata aacaagcggc ctgtaaaaac
     2281 atttacaggc cgcttgtttg aatcacgctc ttgcttacca accttgcaac acgattttgc
     2341 ctatgctgtt gccgctttca atgtcgctgt gggcttgctt caaattggcc acattgatgg
     2401 ggctgatgac tttgttcagc gtggttttga ttttgcctgc gtcgattaag gccgccactt
     2461 ctgccaagat gtcgtgttgg cgcgcgatgt cggcggtgac aaacatgccg cgcgtgaaca
     2521 tgaattccca atggatggaa atggatttgg atttgaacac gcctatgggc aaggctttgg
     2581 ggtcgtctat caacaccaac ttgccttgtg gcgccatcaa tgccgccaat tgctcggcat
     2641 aatcgtcggt gtgttgggta gaaaacaccc agcttggcgc aggcaaacct gttttttgcc
     2701 actcttgcgc caaatcatgg cggtggttga tgacaaaatc cgcacccaat tcctcacacc
     2761 acgcttggct ttcaggccga gaagcagtgg ccaccaccgt caaatccgac aattgcttcg
     2821 ccagctgaat gatttgtgaa cccaccccac ccgagccgcc tataaccaac aacacacggt
     2881 tgcgcatgtc actgcgctca ttcacctgca ggcggtcaaa caagccttcg taagcggtta
     2941 aactggtcaa aggcaaggca gcggcttggg cgaaatccaa gctttgtggc tttttggcga
     3001 tgatgcgcgc gtcaacagct tggtattcgg cattgcaacc tgctcggttg accacgcctg
     3061 cgtaatacac tgcatcgcc
//
LOCUS       DAOMVK010000265         3067 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_9412_length_3067_cov_3.079017, whole genome shotgun sequence.
ACCESSION   DAOMVK010000265 DAOMVK010000000
VERSION     DAOMVK010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9412_length_3067_cov_3.079017"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PK856_08630"
     CDS             complement(<1..233)
                     /locus_tag="PK856_08630"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRL02470.1"
                     /translation="MKKLALAAVLCGVAYSSFAATPVRIETNMGNIDLVLDEKKAPVT
                     VKNFVSYANKGHYNGTVFHRVIDGFMIQGGGMDA"
     gene            complement(271..1260)
                     /locus_tag="PK856_08635"
     CDS             complement(271..1260)
                     /locus_tag="PK856_08635"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HRL02471.1"
                     /translation="MAQTLSAFLQEHAAAHQIDASLIDVMNTLAASCVAISDKVRLGD
                     LAGIIGEAGSDNVQGEHQKKLDVIANDMLIDALQNNPHVAGLASEEEDEFVAAHADGK
                     YLVLFDPLDGSSNIDVNISIGTIFSILPHQGVLSTASFLQAGNQQVASGYALYGPQTL
                     FVLTLRHGVFVFTLNAEEQFVLTRDHCQISAATKEFAINMSNQRHWQSAMQACIAELL
                     QGKEGTRGKDYNMRWVASMVAEVHRILMRGGVFMYPKDNRDPSKAGKLRLMYEANPMS
                     LIVSQAGGLSTNAHQAILDIQPEGLHQRVSVVLGSSEEVTYITDKHIATGMQA"
     gene            complement(1348..2193)
                     /gene="rlmJ"
                     /locus_tag="PK856_08640"
     CDS             complement(1348..2193)
                     /gene="rlmJ"
                     /locus_tag="PK856_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HRL02472.1"
                     /translation="MLSYRHAFHAGNHADMLKHLVLYFVLDYYNRKDKAYTYVDTHSG
                     AGLYDLQAAFAQKIGEYKQGWERLHQATSLPQGLQAFVEHVQSTLENEQSYCGSPWLA
                     ASLLREQDKAKLFELHPSDFDLLRGNIANLHRGRQIQLFKDNGYQGLIGLMPPPSRRG
                     VVLIDPPYETTQDYRAVIDTLKAAMERFATGTYMLWYPLLSRRESIELSGHLQKLAPN
                     NYLQAELWVHAPRADGFGMHGSGMFVINPPFVLAEQLQAALPVLTETLAQDDSAHFVL
                     QHQTA"
     gene            2583..>3067
                     /locus_tag="PK856_08645"
     CDS             2583..>3067
                     /locus_tag="PK856_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02473.1"
                     /translation="MMGLLCFLQKRLFEVTLMKQMIRVLSFAALAGLLAGCGEGYVLI
                     DSKGQVGIEQRNLIYIQIVMMLLVVVPAIAMSLWFAWKYRHNNKAVEKEYAPNWDYSH
                     KIEMWVWAVPCVIILIMAVISWRATHKLDPYRPLDADKEHIQIQAIAAPYQWVFIYPN
                     E"
BASE COUNT          736 a          683 c          799 g          849 t
ORIGIN      
        1 gcgtccatgc cgccgccttg aatcatgaag ccatcaatca cgcggtgaaa cacggtgcca
       61 ttgtaatggc ctttattggc gtaagagacg aagtttttga ccgttacggg ggcttttttc
      121 tcgtctaaaa ccaaatcaat attgcccata ttggtttcaa tgcgcacggg tgtggcggca
      181 aaactgctgt atgcaacacc gcacaatacg gctgccaagg ctaatttttt catgttaagc
      241 gtcctgtaag tcatgaaggg tattgaaaac tcaggcttgc atgcctgtgg cgatgtgttt
      301 gtcggtgatg taagtcactt cttcgctgct gccgagcacg acagacacgc gttggtgcaa
      361 accttcgggt tggatgtcca aaatcgcttg atgggcattg gtagacaagc cgcctgcttg
      421 gctcacaatc aagctcattg ggttagcttc atacatcaag cgcagtttgc ctgctttgct
      481 tgggtcgcga ttgtcttttg gatacatgaa cacgccaccg cgcatcaaaa tgcggtgtac
      541 ttcggccacc atagaggcca cccaacgcat gttatagtct ttgccgcgag tgccttcttt
      601 gccttgcaat aattcggcga tgcacgcttg catcgcgctt tgccaatggc gttggttaga
      661 catgttgatg gcgaactctt tggttgccgc gctgatttgg cagtggtcgc gggtcaacac
      721 gaattgctct tctgcattca aggtgaacac gaacacgccg tggcgcaagg tcaacacaaa
      781 cagggtttgt gggccataca aggcatagcc actggccact tgttggttgc cggcctgtaa
      841 aaagcttgct gtgctcaaca cgccttggtg cggcaagatg gagaagatgg tgccaatgga
      901 gatgttgacg tcgatgttag acgagccatc gagcgggtcg aacaacacca agtatttacc
      961 gtctgcgtgg gcagccacaa attcgtcttc ttcttcagaa gccaagcccg ccacatgggg
     1021 attgttttgc aaggcatcaa tcaacatgtc gttggcaatc acatccaatt tcttttggtg
     1081 ctcgccttgc acattgtcgc tgcccgcttc accgatgatg cctgccaaat cgcccaagcg
     1141 cactttgtcg ctgatggcca cgcatgaggc agccaaagtg ttcatcacat caatcaagct
     1201 ggcatcaatc tgatgcgcgg ccgcatgttc ttgtaaaaat gcactcaatg tttgtgccat
     1261 ggaaaacaat ccttttgttt gaagcggttt ttttaaagtg ggtattttaa agcagtttgc
     1321 ccgaatcggc acggcttaca ggccgcatta tgccgtttgg tgttgtaaaa caaagtgagc
     1381 gctgtcgtct tgcgccagtg tttcggttaa cacaggtaaa gcggcctgta attgctctgc
     1441 caacacaaaa ggcggattga tgacaaacat gccactgccg tgcatgccaa agccatcggc
     1501 gcgcggcgcg tgtacccaca attcggcctg taaataattg ttgggcgcta atttttgcaa
     1561 atggccagac agctcgatgc tttcgcggcg cgagagcagg ggataccaca acatataagt
     1621 gccagtggca aaacgctcca ttgctgcttt caaggtgtcg atgacagcgc ggtagtcttg
     1681 cgtggtttca tacggcgggt cgattaacac cacaccacgg cggctcggcg gcggcatcaa
     1741 gccgattaag ccttggtagc cattgtcttt gaataattgg atttggcggc cacggtgcaa
     1801 gttggcgatg ttgccacgca ataagtcaaa gtcactggga tgcaattcaa acagtttggc
     1861 cttgtcttgc tcgcgcaaga gcgaagcggc cagccacggt gagccgcaat aagactgctc
     1921 attttccagc gtgctttgta cgtgttcgac aaaggcttgt aaaccttggg gtaatgacgt
     1981 tgcttggtgc aagcgttccc agccttgttt gtattcgcct attttttgtg caaaagcggc
     2041 ctgtaaatcg taaagacctg cgcctgagtg ggtgtcgaca taggtatagg ctttgtcttt
     2101 gcggttgtaa tagtccaaaa caaaatacaa aaccaaatgt ttgagcatgt cggcatggtt
     2161 gccggcgtga aaagcgtgac ggtagctgag catggtgcgg ccaaatgtga atacagcagc
     2221 cattgtaacg gttgtgctgc gattaagcac gaaacctcgg ttgtcagtgg gcgcttgata
     2281 aaaatcaaga ttcaagggat ttgaatgtaa atccgtgatt taaaaaactt ttaattgtaa
     2341 atggtgtaac tagcccaagg tttgagttaa ctcaatgatt acaattaaaa tatttgatat
     2401 gcatcaggct ttgagctggg ctgtctgtct gtttttgtgg ggcagggggc agcccaattg
     2461 cttgtaaacc atgaaattta acccagttcc tgttataagt taacgacttg gtagcactta
     2521 acctcatgat gttgttttta ctgtgtttgc tgttaatcaa tggtgaataa cagtaagcct
     2581 gcatgatggg gttgttgtgt tttttacaaa aaagattatt tgaggtcacg ctcatgaagc
     2641 agatgattcg ggtcttatct tttgctgcgc ttgccggttt attggcgggt tgtggtgagg
     2701 gatatgtgct gatcgattcc aagggacagg ttggtatcga gcagcgtaat ttgatctaca
     2761 tccaaattgt gatgatgttg ttggtggtgg ttcctgccat tgcaatgtcg ttgtggtttg
     2821 catggaagta tcgccataac aacaaagcag tagaaaaaga gtatgcgccc aattgggact
     2881 attctcacaa gattgaaatg tgggtttggg ctgtcccttg tgtcattatt ttgattatgg
     2941 cggtcatttc atggcgtgct actcacaaat tggatccata ccgtcccttg gatgccgaca
     3001 aagagcacat tcaaattcaa gccattgccg caccttatca atgggtattc atttacccta
     3061 atgagaa
//
LOCUS       DAOMVK010000266         3066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_9417_length_3066_cov_3.017602, whole genome shotgun sequence.
ACCESSION   DAOMVK010000266 DAOMVK010000000
VERSION     DAOMVK010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9417_length_3066_cov_3.017602"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="PK856_08650"
     CDS             complement(<1..1060)
                     /locus_tag="PK856_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL02474.1"
                     /translation="MKNTDVQASHMEVQQFIDAQPLSRTQKLIIALCFLVVAIDGFDT
                     AAVGFIAPALKEAWQLDPEHLMPLFGAGLFGLMTGALIFGPLADKIGRKPILVGSVFV
                     FGLASLLSAFSPDMTTLVVLRFITGIGLGGAMPNAITLTSEYCPSARRSTLVTLMFCG
                     FTVGSALGGIVSAQLLSVVDWYGILIIGGVLPLLTLPFLMWLLPESLRFMVLQNQPEH
                     QIKATAQRLLPAGEAQVPSLVASRNEKAKVSVTELFSKDYAVGTMLIWLAFFMSLLII
                     YLISSWMPTLLKDVGFDLKNASWITSVFQIGGTVGAIMLGFLMDRLGASKVLCAAYVL
                     GAVFVVMVGQGITSLNMAL"
     gene            complement(1231..1935)
                     /locus_tag="PK856_08655"
     CDS             complement(1231..1935)
                     /locus_tag="PK856_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRL02475.1"
                     /translation="MSYVFEPMKVVGLPVHGSDDLYPVRRVYCVGRNYAAHAREMGFD
                     PDREPPFFFCKPNDAESIVPVKAGASVDLPYPSLTANYHYEIELVVAIGKGGKDIAVE
                     QAAEHIFGYAVGLDMTRRDLQMKMREMGRPWEIGKAFDFSAPIGELHPIAETGEINTA
                     DIHLTVNGKTAQHSDISHLIWNVAETIAKLSELFELKAGDLIFTGTPEGVGAVVRGDV
                     MHAEVAGLSAITVNVK"
     gene            complement(1966..2619)
                     /gene="maiA"
                     /locus_tag="PK856_08660"
     CDS             complement(1966..2619)
                     /gene="maiA"
                     /locus_tag="PK856_08660"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HRL02476.1"
                     /translation="MNMKLYNFFRSGTSHRTRIALNLKGLEYEYVAVALGKEEHLQAA
                     FQAVNPQGLVPALVLDDKTTLIQSPAIIEWLEEVFPQNPLLPAEPLARAHVRAMAAIV
                     GCDIHPLNNRRILQYLRGELQQSEEDVLKWCARWISAGFAALEELLAGDTERGDFCYG
                     NRPGLTEVYLIPQVESARRFNVDLSPYPNIVAIDERCAQIKAFADAAPWVQVDAPQH"
     gene            complement(2600..>3066)
                     /locus_tag="PK856_08665"
     CDS             complement(2600..>3066)
                     /locus_tag="PK856_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HRL02477.1"
                     /translation="AHQLIELPKSWKRWATADREPIDTWTFGRVTLLGDAAHPTTQYM
                     AQGACMAMEDAVTLGEALRVHDNDIAKAFDTYQRARVARTARIVLSSRQMGKIYHAKG
                     VERMIRNDLWRGRSAERFYDAMEWLYGWNVNNCLDAAQHYTGKAQQHEHETV"
BASE COUNT          789 a          843 c          714 g          720 t
ORIGIN      
        1 ataaggccat gttcaaactg gtgatgcctt gacccaccat caccacaaac accgcgccca
       61 acacataagc cgcgcacaag actttgcttg cgcccaatct gtccatcaaa aagcccaaca
      121 ttatcgcgcc caccgtgcca ccgatttgga acacggaagt aatccacgag gcatttttca
      181 aatcaaaacc cacgtcttta agcaaggtcg gcatccagct ggaaatcaaa tatatgatga
      241 gcaagctcat gaagaatgcc agccaaatca gcatggttcc cactgcatag tctttgctga
      301 acagctcggt cacgctgact ttggcttttt catttctcga tgccaccagt gatggcactt
      361 gagcctcacc tgcgggcaac aaacgctggg ccgtggcttt gatttgatgt tcaggttggt
      421 tttgcaacac cataaagcgc agcgattcag gcaaaagcca cattaagaag ggtaaggtta
      481 acaagggcaa cacaccgcca ataatcaaaa taccatacca atccaccacg ctgagcaatt
      541 gcgccgacac gatgccgccc aaggccgagc ccacggtaaa gccacaaaac atcagcgtca
      601 ccaaagttga gcgacgggcg ctggggcaat attcagaagt taaggtaatc gcattgggca
      661 tggcgccgcc caagcctatg cctgtgatga agcgcaacac caccaaagtg gtcatgtcgg
      721 gtgaaaacgc cgacaacaaa ctggccaaac caaacacaaa caccgagccc accaaaatgg
      781 gtttacgacc aattttgtct gccaaggggc caaatatcag tgcgccagtc atcaagccaa
      841 acaagccagc accaaacaat ggcatcaaat gctcgggatc caattgccac gcttctttca
      901 atgcgggggc gataaagccc actgcggcag tatcgaaacc atcgatcgcc accaccaaaa
      961 aacacagtgc aatgatgagt ttttgcgtgc gtgacaatgg ctgtgcatca atgaattgct
     1021 gcacctccat gtgcgacgct tgtacgtcgg tattcttcat tttcttcttc ctttttggca
     1081 ttgtttaatc acaatttgtt tttttaaata cgtgttttaa agcggtattg cataaattga
     1141 gggcgcaaaa aaagaaccaa tagcctcatc gcattggctc ttgtggtcac aagcggcctg
     1201 taaagccact tgtgttgcgg caaatcagcg ttacttcaca ttcacggtaa tcgcgctcaa
     1261 tcccgctact tcggcgtgca tcacatcgcc gcgcaccacc gcccccacgc cctcaggtgt
     1321 gccagtgaat atcaaatcgc ctgctttcaa ttcaaacaat tcggacaatt tggcaatggt
     1381 ttcggccaca ttccaaatca aatggctgat gtcgctgtgt tgagccgttt tgccgttcac
     1441 ggttaagtgt atatcggctg tgttgatttc gcctgtttcc gcaatgggat gcaattcgcc
     1501 tatgggtgca gaaaaatcga atgccttgcc tatctcccaa gggcggccca tttcacgcat
     1561 tttcatttgc aaatcgcgac gggtcatgtc caagcccacg gcgtagccaa aaatgtgctc
     1621 agccgcctgt tccactgcaa tgtctttgcc gcctttgcct atggccacca ccaattcgat
     1681 ttcataatgg tagtttgccg tcaaacttgg gtaaggcaag tccacgctgg cgcctgcttt
     1741 aacaggcacg atggattcgg catcgttggg tttgcaaaag aaaaacgggg gttcacgatc
     1801 agggtcgaaa cccatttcgc gtgcatgcgc cgcataattt cggcccacac aatacacacg
     1861 gcgcactggg tacaaatcat cactgccgtg tacaggcaaa ccaaccactt tcatgggttc
     1921 aaatacataa gacatacaat tcctttttta actggataac gaaaactagt gttgcggtgc
     1981 gtccacttgc acccatggtg cagcatcggc aaaagccttg atttgcgcgc atcgctcatc
     2041 aatggccaca atattggggt aagggcttaa atccacattg aagcgacgtg ccgattccac
     2101 ttgcggaatc aaatacactt cggtcaaacc tgggcgattg ccgtagcaaa aatcaccgcg
     2161 ctcagtgtct cccgccaaca attcctccaa agcagcaaaa cccgcactga tccagcgcgc
     2221 acaccatttc aacacatctt cttcagactg ctgcaattcg ccgcgcaagt attgcaagat
     2281 gcggcggtta ttaaggggat ggatatcgca gcccacgatg gctgccatgg cgcgcacatg
     2341 cgctcgtgcc aaaggctcgg cgggcaacaa tgggttttgt ggaaacactt cttccagcca
     2401 ctcgatgatg gcagggcttt ggatcaaagt ggttttatcg tctaaaacca atgcaggcac
     2461 caaaccctgt gggtttaccg cttgaaatgc ggcctgtaaa tgctcttctt tgcccagcgc
     2521 caccgccaca tattcgtatt ccaagccttt caaattcagg gcgatgcggg tacggtggga
     2581 agtgccactg cggaagaagt tatacagttt catgttcatg ttgttgtgcc ttacctgtgt
     2641 aatgttgggc tgcatcgaga cagttgttga cgttccagcc atacaaccac tccatggcat
     2701 cgtaaaaacg ctctgctgag cgaccgcgcc acaaatcatt gcgtatcatg cgctctaccc
     2761 ctttggcatg gtaaatcttg cccatttgac gggaagacaa cacgatgcgg gcggtgcgag
     2821 cgacacgggc gcgttgatac gtgtcaaaag cttttgcgat gtcattatca tgtacacgca
     2881 aagcctctcc caaggttact gcgtcttcca tggccataca agcaccttgc gccatgtatt
     2941 gcgtggttgg gtgggcggcg tcgcccaata aggtcacgcg gccaaacgtc catgtgtcta
     3001 tcggctcgcg gtcggcagtg gcccagcgtt tccacgattt tggcaattca atcaattggt
     3061 gcgctt
//
LOCUS       DAOMVK010000267         3064 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_9421_length_3064_cov_3.435028, whole genome shotgun sequence.
ACCESSION   DAOMVK010000267 DAOMVK010000000
VERSION     DAOMVK010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9421_length_3064_cov_3.435028"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="PK856_08670"
     CDS             complement(<1..888)
                     /locus_tag="PK856_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HRL02478.1"
                     /translation="MHITATELRSLSQNILAAHGFSKEHQHSITETLVTAQIDQCHSH
                     GVWRLLGIIDTLKHGKMDATASPVVSHEQGAIIKIDAKLGAATTALALGLPKLIEKAK
                     QSGIALLAVNHCVHFSALWVEIEQLTRAGLIGINMTPSHAWVAPAGSREPLLGTNPLG
                     FGFPRRNPDEPYVFDFATSATARGEIQLHDRNGTPLPEGVAIDADGQPTTDPKAAMAG
                     AMLTFGGHKGSAISTMVELLAGIAIGDMTSQESMAFADGTPTLPYGGEIIIAIDPVML
                     LGEDVDSLYARGDAYLNQFN"
     gene            complement(909..1523)
                     /locus_tag="PK856_08675"
     CDS             complement(909..1523)
                     /locus_tag="PK856_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02479.1"
                     /translation="MITKFRHLERYPLWFEIAPLAITAAIMAVQRFLFTYVITYLSFT
                     IGICITSLFLPLKHPHWAIMKIGLPAVLIMMCGGLLFSIWIAASTTPTMVNTGLSILA
                     PTILLPATCLLLGLVSLTTATSWGTVGLPMMGIGHDIGTLTVLYALFAIFCWLSPLFG
                     ILSALTGWFALKTTLVSKAELPLQKQTESLISGYRNMPKITSLA"
     gene            complement(1617..>3064)
                     /locus_tag="PK856_08680"
     CDS             complement(1617..>3064)
                     /locus_tag="PK856_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRL02480.1"
                     /translation="QNGAHTVRSPIDGSTLAKVNYLPAAEVGQHIAASQTAFEAWRKI
                     PAPRRGELVRILGEVLREHKADLAQVVTLEAGKITQEALGEVQEMIDICDFAVGLSRQ
                     LYGLTIASERPGHHMRETWHPVGPVGIISAFNFPVAVWSWNAALALVCGNSIVWKPSE
                     KTPLTAIACQALFEKAVARFGSDAPANLSTLIIGDRDTGEALVNDARVPLVSATGSTR
                     MGREVGTKVAARFGRSILELGGNNAMILAPSADVELATRAILFSAVGTAGQRCTTLRR
                     LIVHKDLKAQVLERLKKSYQSVSIGNPTENHLVGPLVDAASFNMMQDALTQARSEGGT
                     VFGGERQLADTYPDAFYVAPALVEMPAQSDVVKHETFAPILYVMEYEHFEDALRLNNE
                     VPQGLSSCIFTTDMREAEIFQSATGSDCGIANVNIGTSGAEIGGAFGGEKETGGGRES
                     GSDAWKAYMRRQTNTINYSRELPLAQGIKFD"
BASE COUNT          735 a          771 c          832 g          726 t
ORIGIN      
        1 gttgaattgg ttcaaatacg catcgccgcg cgcatacaag ctgtccacgt cttcgcccaa
       61 taacatcaca ggatcgatgg cgatgatgat ttcaccgcca taaggcaaag tgggcgtgcc
      121 gtcggcaaac gccatcgatt cttggctggt catgtcgcct atggcgatgc ctgcgagcaa
      181 ttccaccata gttgatatag ccgagccttt gtgcccgcca aacgtcagca tcgcgccagc
      241 catcgccgct ttcgggtcag tggtgggttg tccatcggca tcaatcgcca cgccctcagg
      301 taagggagtg ccattgcggt cgtgcaattg gatttcaccg cgcgcggtgg cactggtggc
      361 aaagtcaaac acatacggtt cgtcaggatt gcggcgtgga aagccaaagc caagtggatt
      421 ggtacccaat aaaggctcgc ggctgcccgc tggcgcgacc caagcatggc ttggggtcat
      481 gttgatgcct atcaaccctg cacgggtcag ttgttcgatt tctacccaca aggctgaaaa
      541 atgcacgcag tgattcaccg caagcagggc gatgccgctt tgcttggctt tttcgattaa
      601 tttgggcaaa cccaatgcca aagccgtggt ggctgcaccg agtttggcat cgattttgat
      661 gatggcgcct tgctcgtggg agactacggg gctggcagtg gcatccattt tgccgtgttt
      721 taaggtatcg atgatgccaa gcaaacgcca tacgccgtgc gaatggcatt ggtcgatttg
      781 agcggtgacc aaggtttcgg tgatgctgtg ctggtgttct ttgctaaagc cgtgggctgc
      841 taagatgttt tggcttaagc tgcgcaattc ggttgcggta atgtgcatgt ttcttcctag
      901 gtagttgttc acgccaaaga ggtaatcttt ggcatatttc ggtatccgct gatcagtgat
      961 tcagtttgtt tttgcagggg caattctgct ttggaaacca gcgtggtttt gagggcaaac
     1021 cagccagtta atgcagacag aataccgaat aatggggata accaacagaa gattgcaaac
     1081 agtgcatata agacagtgag tgtgcctatg tcatggccta tgcccatcat gggcaaaccg
     1141 accgtgcccc aagaagtggc tgtggtcaaa gacaccaagc caagcaatag acaggtggca
     1201 ggtaacaaga tggtaggagc caatatgctt aaacctgtgt tcaccatggt gggcgtcgtg
     1261 cttgctgcaa tccagatgga aaacaacaag cccccacaca tcataatcaa taccgcaggc
     1321 aaacctattt tcatgatggc ccagtgtggg tgttttaaag gcaagaataa gcttgtgatg
     1381 caaataccaa tggtgaagga aaggtaagta atgacatatg tgaaaagaaa gcgttgtact
     1441 gccatgatgg cagcggtgat tgccaaagga gcgatttcaa accaaagcgg gtaacgctct
     1501 aagtggcgaa acttggtaat catgatgcgc tcctaaaaaa gggcaataat cacccttggc
     1561 tgttgccatg aagacaacag ccaaaagcta acagaggtag gatttacagg ccgctatcag
     1621 tcaaatttaa tgccttgagc caatggcaat tcgcgtgaat agttgatggt attggtttga
     1681 cgacgcatat aggctttcca tgcgtctgag ccagattcac ggccgccgcc ggtttctttt
     1741 tcgccaccaa atgcgccgcc aatttccgcg ccgctggtgc caatgttgac gttggcaatg
     1801 ccgcaatcgc tgcctgtggc gctttggaag atttccgctt cacgcatgtc agtggtgaaa
     1861 atgcaagaag acagaccttg aggaacttca ttgttcaagc gcaacgcgtc ttcaaagtgc
     1921 tcgtattcca ttacatacaa aatcggcgcg aaggtttcgt gtttgaccac atcgctttgc
     1981 gcaggcattt ccaccaaagc aggggcaacg taaaacgcat cagggtaggt gtcggccaat
     2041 tggcgctcgc caccaaacac ggtgccgcct tcgctgcggg cttgggtcaa cgcgtcttgc
     2101 atcatgttga agctggcggc atccaccaat gggccaacca aatggttttc ggtagggtta
     2161 ccgatggata ccgattggta agattttttc aggcgttcta atacttgggc tttcaagtct
     2221 ttgtgcacga tgaggcggcg caaagtggtg cagcgttggc cagcagtgcc cacggcgctg
     2281 aacaaaatgg cgcgagtggc caattccaca tcggcgctcg gtgccaaaat catcgcgtta
     2341 ttgccgccca attccaagat agagcgacca aaacgtgcag ccacttttgt gcccacttca
     2401 cggcccatgc gtgtgctgcc tgtggcgctc accaagggta cacgcgcatc gttcaccaag
     2461 gcttcgcctg tgtcgcgatc gccaatgatt aaggtagaaa ggttggcagg ggcatcgctg
     2521 ccaaaacgtg ccacggcttt ttcaaacaag gcttggcaag cgatggcggt caatggggtt
     2581 ttttccgatg gtttccacac aatcgaattg ccgcacacca atgccaaagc cgcattccac
     2641 gaccatactg ccacaggaaa attgaaggca ctgatgatgc ccacagggcc cactgggtgc
     2701 cacgtttcac gcatgtggtg gcctgggcgc tcagaggcga tggtcaagcc atacaattgg
     2761 cgcgacaagc ccacggcgaa atcacaaata tcaatcattt cttgcacttc acccaaggct
     2821 tcttgggtga ttttacctgc ctctaaagtc actacttgtg ccaaatcggc tttgtgctcg
     2881 cgcaatacct cacccaagat gcgcaccaat tcgccgcgac gcggtgctgg aattttgcgc
     2941 caagcttcaa acgcggtttg agatgcagcg atgtgttggc ccacttcggc ggctggcaaa
     3001 taatttacct tggccaaagt gctgccatcg atggggctgc gcacagtgtg tgcgccattt
     3061 tggt
//
LOCUS       DAOMVK010000268         3062 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_9432_length_3062_cov_1.906884, whole genome shotgun sequence.
ACCESSION   DAOMVK010000268 DAOMVK010000000
VERSION     DAOMVK010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9432_length_3062_cov_1.906884"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="PK856_08685"
     CDS             <1..401
                     /locus_tag="PK856_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02481.1"
                     /translation="EVYPKFQAFLGSPDIIQATEIKQLLQFLVLGVNSMGARIRYYKN
                     VESFVAKLLIDNDEEHIKSGLIYLLYGQDEVKYRMVELIEGNRRIIGFGRSAITEILG
                     WLNQDELPIINEKITKVMSYFGYDVEQYAE"
     gene            complement(555..1037)
                     /locus_tag="PK856_08690"
     CDS             complement(555..1037)
                     /locus_tag="PK856_08690"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HRL02482.1"
                     /translation="MSLTAADYNYLRLCVDLAQTALYAGDEPFGSVLVNQFGDILYQD
                     HNHVASGDATQHPEFAIARWAAVNLSPEERAHCVVYTSGEHCPMCAAAHAWVGLGKIV
                     YATSGQQLRAWLQEWQAPLVPVAALSITDVAPNIETAGPVEEFEEEMRDLYRQAFQKQ
                     "
     gene            complement(1141..2619)
                     /gene="proP"
                     /locus_tag="PK856_08695"
     CDS             complement(1141..2619)
                     /gene="proP"
                     /locus_tag="PK856_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HRL02483.1"
                     /translation="MRGLKRNKPVQISDIKIIDKDKSRKAITAAALGNAIEWFDFGVY
                     GFVAFALGQVFFPNASPSVQMIAALATFSIPFVFRPLGGIVFGAMGDKHGRQKILAVT
                     IILMSISTFAIGLIPSYASIGIWAPILLLLCKVAQGFSVGGEYAGAAIFVAEYSPDRK
                     RGFMGSWLDFGSVAGFVLGAGVVSLLQLSLSDAAFLQWGWRVPFFLALPLGIIGLYLR
                     HALDETPVFQEHSETKAAAAPALGFKHIFKTHKRSLLVCVGLVLVTNVTYYMLLTYLP
                     SYFSHNLGYSADHGVLIIIAVMIGMLFVQPIIGALSDRYGRRPFFFIGSLALVVLPLP
                     AFWLLATNVPVMVFLGLLGLALSLNMLIGVMAATLPALFPTAIRYTGLAIAFNVSVVI
                     AGLTPTITSTLVEMTHNLYVPAYYLIVFGFLGLVATVAMPETANKPLRGSAPMASNKA
                     EARELLEEHHDRIEERIEDIDQQIAALQTLRQKLANQHPQIN"
BASE COUNT          827 a          652 c          820 g          763 t
ORIGIN      
        1 atgaggttta tccaaaattt caggcatttt taggttcccc tgacattatt caagccacgg
       61 aaatcaaaca attgctccag tttttggttt tgggtgtgaa ttctatgggg gcacgcatac
      121 gttattataa aaatgttgaa tcattcgtgg ccaaactgtt gatagacaat gatgaagagc
      181 atattaaatc tggtttgatt tatttgcttt atggtcagga tgaagttaaa taccgtatgg
      241 tagagttgat agagggtaat cgcagaatca ttggatttgg acgttcggcc atcacggaaa
      301 tattgggctg gttaaaccaa gatgaactgc ccattatcaa tgagaaaatc accaaagtga
      361 tgagttattt tggatacgat gtagagcagt atgcagagtg atgagcaaat ttgattaaat
      421 agcgaatcaa agtaaaagtg gcctgtaaac cttgtgaata aggcttacag gccgctttaa
      481 tatttgggta atgtgttcag cagagaatga aggagtctaa ctctcaatat gggtttttga
      541 atttgcagtc tgattcactg cttttgaaac gcctgtcggt acaaatcgcg catttcctct
      601 tcaaactctt ccactggccc tgcggtttcg atattggggg cgacatcggt aatggataag
      661 gcggcaacgg ggacgagtgg ggcctgccat tcttgcagcc aagcgcgcaa ttgttggccc
      721 gaggtggcgt aaacgatttt gcctaagccc acccaagcat gggcggcggc acacatgggg
      781 caatgttcac ctgaggtgta gaccacgcaa tgggcgcgct cttcggggct gagattgaca
      841 gcggcccagc gtgcgatggc aaattctggg tgttgggtgg catcgccgct ggcaacgtgg
      901 ttgtggtctt ggtagaggat gtcgccaaat tgattcacca acacggagcc aaatggctca
      961 tcgcctgcgt acagggcggt ttgggcgagg tcgacacaga ggcgcaagta gttgtaatcg
     1021 gcagcggtga gtgacatggc gggctcctgt gtggatgagg tttgacactc attgcaacag
     1081 aagcaccagc caacgtcaat gggggcagac ataccttgtt tataacaagg tatgttgatt
     1141 tcagttgatt tgagggtgtt ggttggcgag tttttgacgc agtgtttgta aggcggcaat
     1201 ttgttggtct atgtcctcaa tgcgttcttc tatgcggtca tggtgttctt ccaacaattc
     1261 acgggcttct gctttgttgc tggccatggg ggcgctgcct cgcaatggct tattggcagt
     1321 ttctggcatg gccactgttg ccaccaaacc caagaatcca aacacaatca ggtaataagc
     1381 aggcacatac agattgtgag tcatttctac caaggttgag gtgatggtgg gggttaaacc
     1441 tgcaatcact acggatacgt taaaggcaat ggccaaacct gtgtagcgga tggcggtggg
     1501 gaataaggct ggcagggtgg cagccatgac accaatcaac atattcaaag acagggccaa
     1561 acccaacaat cctaaaaaca ccatgacggg cacattggta gccaataacc aaaacgcagg
     1621 caagggcaaa acaaccaagg ccaagctgcc gatgaaaaag aaagggcgac ggccataacg
     1681 atcgctcaat gctccgatga tgggctgcac aaacaacatg cctatcatca ctgcaatgat
     1741 gattaacacg ccatggtcgg ctgaatatcc caagttgtga gagaaataac tcggtaaata
     1801 cgtcaacaac atgtagtaag tgacgttggt gaccaaaacc aatcccacac acaccaacaa
     1861 gctgcgtttg tgggttttga agatgtgttt gaaacccaat gcaggtgcgg ccgcagcctt
     1921 ggtttcgctg tgctcttgaa acactggggt ttcatccaag gcatggcgca aatacaggcc
     1981 gatgatgccc aatggcaaag ccaagaaaaa aggcacgcgc cagccccatt gcaagaatgc
     2041 cgcatcgctg agtgacaatt gtaataaaga caccaccccc gcgccgagca caaagcctgc
     2101 cactgagccg aaatccaacc aactgcccat aaagccacgt ttgcggtcgg gagagtattc
     2161 cgctacaaat atcgcggcac ccgcatattc cccgccaaca gagaagcctt gggcgacttt
     2221 acacagcagc aatagaatcg gtgcccaaat gcctatgctg gcgtaggagg gaatcaggcc
     2281 aatggcgaag gtgctgatag acattaaaat gatggtgacg gccagaattt tttggcgccc
     2341 atgtttgtct cccatcgcgc caaacacgat gccgccaagc ggcctgaaca caaatggaat
     2401 ggagaaggtg gccaaagcgg caatcatttg cacgctgggg gaggcattgg ggaagaatac
     2461 ctgccctaag gcaaaagcaa caaagccata cacgccaaag tcgaaccact caatggcatt
     2521 gcccagtgcg gcagcggtga tggccttgcg gcttttgtct ttgtcgatga ttttgatgtc
     2581 gctgatttgg acaggtttgt ttctttttaa tcctcgcatg tggtttccta tttaattttt
     2641 tggctggcaa caaaatgcgt tgcagttact gaattacata gatgaacaga ttgggctgca
     2701 cccaagacgc aaatctaaaa caatgtggct aaaatgtggg tcaaagctat aggcttatgt
     2761 cgcctaagct tcaacggaca agcagttttg ccattaggct aaaacaggct catagtgggc
     2821 attgccactg gtttgtgtga ctgagacgaa agctagtgtt ccgcttgaaa gtctgatatt
     2881 caataaaatt gaaacagtgg gttttggtga aggtcatgca tcaccgtttt ttaccttagc
     2941 ataacttgct attaatttgc aaacttgtgg gctttattgg catttaaggt tgaagtttgg
     3001 tgaagttggg taataagcgt tcgctgggtg agggtgtttt gtttgctgag gggtgatggg
     3061 ag
//
LOCUS       DAOMVK010000269         3057 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_9452_length_3057_cov_2.962025, whole genome shotgun sequence.
ACCESSION   DAOMVK010000269 DAOMVK010000000
VERSION     DAOMVK010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9452_length_3057_cov_2.962025"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PK856_08700"
     CDS             complement(<1..226)
                     /locus_tag="PK856_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02484.1"
                     /translation="MLDASDILTFFGLVFLFAMVMGVVIAVCAAKILFYKRHHKYSVP
                     AAIVLVLGWYAYEIVPPIWQNYQATKAFKKK"
     gene            complement(574..1383)
                     /locus_tag="PK856_08705"
     CDS             complement(574..1383)
                     /locus_tag="PK856_08705"
                     /inference="COORDINATES: protein motif:HMM:NF016818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-interacting protein"
                     /protein_id="HRL02485.1"
                     /translation="MSSNQSQLQRVRHDIKIRRLRVEKIEDLSASLKRFTFSGDDLDS
                     FASASFDDHLKAFFPNAAGELNLPQVGKKGVVFPEGVAKPAVREYTPTGIDLAGKRLA
                     LEFAIHDAGPATSWAQTAQVGDEIGIAGPRGSIVLPMDLQHYILIGDETALPAIRRRL
                     GELPASANAHVIAEVNGAADETALPSAATVSATWVHRAGKAAASEAFMLPVLANLQLP
                     QADVQVWIACEREQARQIREWFLEQGWDKHAVRYASYWNKGTAAYHEPHED"
     gene            complement(1442..3052)
                     /locus_tag="PK856_08710"
     CDS             complement(1442..3052)
                     /locus_tag="PK856_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003683571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HRL02486.1"
                     /translation="MTMQFGAKPLFENVSVKFGEGNRYGLIGANGSGKSTFMKILGGD
                     LEQSSGEVAIDNGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAIYANMEA
                     TEDDYMRAAELEGKFAEYDGYTAEARAGALLSGVGIEEALHQANMSEVAPGFKLRVLL
                     AQALFSKPDVLLLDEPTNNLDINTIRWLEDTLNQYDSTMIIISHDRHFLNEVCTHTAD
                     VDYGTVTTYPGNYDDYMLASAQSRERAMKDNAKAKEKLQELQEFVARFSANKSKARQA
                     TSRLKQADKIKDEMIEVKPSTRQNPYIRFEPDEKAKLHRHAVEINGLSKGFDKQLFKD
                     ISFIMEAGERLAIIGPNGAGKSTLLKLLAGAFNPEYAEGVVADKGLVKWAEKANIGYY
                     PQDHENDFDCNDDLTTWMRQWGLEGDDEQVIRGTLGRLLFGSNDVTKNVRVLSGGEKG
                     RMLYGKLILQKPNVMVMDEPTNHMDMESIESLNMALDKFPGTLIFVSHDRQFVSSLAT
                     QILELDGEGGYDYFLGDYETYLSKKGVI"
BASE COUNT          707 a          811 c          725 g          814 t
ORIGIN      
        1 attttttctt gaaggcttta gtggcttgat agttttgcca tattggaggc acgatttcat
       61 aggcgtacca tcccaatacc aatacaattg ccgcgggcac tgaatatttg tgatggcgtt
      121 tgtaaaacaa aattttggca gcgcaaacag caatgaccac acccatgacc atcgcaaaca
      181 aaaacactag gccaaaaaat gtcaaaatat cagaagcatc aagcatgaca ttcccttatt
      241 tggtttcaac acttgttctc aagccatgca cgcgatggtt tatccaccct cagccatgat
      301 ccatttcgtt tttcaaacag cagaagtgca cgtttagcat tatggtcaag cttgtgattc
      361 ccttgtgatg tttcgcccat ttctggccat ttttatggct gtaaagccac aatggtaatg
      421 cacgatgcca aaccgatggg caaacccagc tactgctttt aagcaaaaac aagcggcctg
      481 taagtgttta caggccgctt gtttgggcat tttggtgtgt tttgctaagg ctttatcaac
      541 gcctctacta caccttactc atgcgatgaa tgctcaatct tcatgtggct cgtggtaggc
      601 cgcggtgcct ttgttccaat aactggcgta gcgcactgcg tgtttgtccc agccttgttc
      661 caaaaaccac tcgcggattt ggcgcgcttg ctcgcgctca cacgcaatcc acacttgcac
      721 atcggcttgc ggcagctgca aattggccaa cacaggcaac atgaacgctt cgcttgccgc
      781 ggctttgcca gcgcggtgca cccaagtcgc gctcacagtg gcagcgcttg gcaaggcggt
      841 ttcatcggcg gcaccattca cttcggcaat gacgtgcgcg ttggcactgg cgggcaattc
      901 acccaagcgg cggcggatgg cgggcaaggc ggtttcatca ccgataagga tgtagtgttg
      961 caaatccatg ggcaatacta tggagccgcg tgggccggca atgccgattt catcgcctac
     1021 ttgcgcggtt tgcgcccaag aggtggctgg gcctgcatcg tggatggcaa attccaaagc
     1081 caagcgtttg cctgccaagt ctatgcctgt gggcgtgtat tcgcgcaccg caggtttggc
     1141 cacgccttcg ggaaacacca cccctttttt acccacttgt ggcaggttca attcgcctgc
     1201 ggcattgggg aaaaacgctt tcaaatggtc gtcgaagctg gcgctggcaa agctgtccaa
     1261 atcgtcgccg ctgaaggtaa agcgcttcaa gctggcgctc aagtcttcga ttttttccac
     1321 gcgcaaacgg cggattttga tgtcgtgacg cacacgttgc aactgagatt gattgctact
     1381 cattatcatt actcctgttt aaaaaagcgg cctgtaagcg tttacaggcc gctttaaaag
     1441 cttaaatcac gcctttttta gacaaataag tctcgtagtc gcccaagaag tagtcgtaac
     1501 caccctcacc gtccaattcc aaaatttgtg tggccaaaga gctcacaaat tggcggtcgt
     1561 gcgacacaaa aatcaaggtg cctggaaatt tgtccaaagc catgttcaat gattcgatgc
     1621 tttccatgtc catgtggttg gtcggctcgt ccatcaccat cacgttgggc ttttgcaaaa
     1681 tcaatttgcc atacaacatg cggccttttt caccaccaga caacacgcgc acgtttttgg
     1741 taacgtcatt gctgccaaac agcaagcggc ccaaagtgcc acggatgact tgctcgtcgt
     1801 cgccttccaa gccccattgg cgcatccaag tggtcaagtc gtcgttgcaa tcgaaatcgt
     1861 tttcatggtc ttgtgggtaa tagccgatgt tggctttttc tgcccatttc accaagcctt
     1921 tgtccgccac cacgccttcg gcgtattcag gattgaatgc acctgcgagc aatttcaaca
     1981 aggtggattt gcctgcgccg ttagggccga tgatcgccaa gcgctcgccc gcttccatga
     2041 tgaagctgat gtctttgaac aattgtttgt caaagccttt gctcaagcca ttgatttcca
     2101 ccgcatggcg gtgcaatttg gctttttcat cgggctcaaa acggatgtaa gggttttgac
     2161 gggtagacgg ttttacctca atcatttcgt ctttaatttt gtccgcttgt ttcaaacgtg
     2221 aagtggcctg acgggctttg gatttattgg cagagaaacg cgccacgaat tcttgcaatt
     2281 cttgcaattt ttctttggct ttggcgttgt ctttcatcgc gcgttcgcgt gattgcgcgc
     2341 tcgcaagcat gtaatcgtcg taattaccag ggtaagtggt gacggtgcca taatccacgt
     2401 cggcggtatg tgtgcagacc tcattcaaaa agtgacggtc atgcgaaatg atgatcatgg
     2461 tggaatcgta ttgattcaaa gtgtcttcca accagcggat ggtgttgatg tccaagttat
     2521 tggtgggctc gtccagcaac aacacatctg gtttggagaa caacgcctgc gccaacaaca
     2581 cgcgcagttt aaagcctggt gccacttcgc tcatgttcgc ttggtgcaag gcttcttcta
     2641 tgcccacgcc tgagagcaat gcgccggcac gcgcttcagc ggtatagcca tcgtattcag
     2701 caaatttgcc ttccaattcg gccgcacgca tgtagtcgtc ttcggtggct tccatattgg
     2761 cataaatggc atcgcgttcg gtcatggcgg cccacatctc agtgtggccc atcatcacca
     2821 catccaatac acgcatgtct tcatacgcaa actgatcttg gcgcaactta cccaaacgca
     2881 caccattatc aatcgccacc tcgccactgc tttgctccaa atccccaccc aatatcttca
     2941 taaaagtgga tttgcctgag ccattggcac cgatgagacc atagcggtta ccttcgccaa
     3001 atttcaccga cacattttca aacaaaggct tggcgccaaa ctgcatggtc aaattgt
//
LOCUS       DAOMVK010000270         3055 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_9459_length_3055_cov_2.802000, whole genome shotgun sequence.
ACCESSION   DAOMVK010000270 DAOMVK010000000
VERSION     DAOMVK010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9459_length_3055_cov_2.802000"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            343..624
                     /locus_tag="PK856_08715"
     CDS             343..624
                     /locus_tag="PK856_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02487.1"
                     /translation="MEVLTDLKAILHSKRANIYYLEHCRVMQKDGRVLYLTESKKQNF
                     YWNIPIANTTVIMLGTGTSITQAAMRMLASAGVLVGFLWRWRYAFVCRC"
     gene            608..1300
                     /gene="cas1f"
                     /locus_tag="PK856_08720"
     CDS             608..1300
                     /gene="cas1f"
                     /locus_tag="PK856_08720"
                     /inference="COORDINATES: protein motif:HMM:TIGR03637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated endonuclease Cas1f"
                     /protein_id="HRL02488.1"
                     /translation="MFAGAEIEWLTPQSEYRPTEYVQGWLSFWFDEAKRLEVAKSLQL
                     QRLAFLQQVWRKDKDLQQAGFGSEDAVLQRGFQSFEAAIARSQKVGDLLLNEAALTKK
                     LYHFAAQTCRIQDFNRSHENGDLANQFLSHGNYLAYGLAACTLWVLGIPHGFAVMHGK
                     TRRGALVFDVADLVKDAIVLPMAFMCASENASEQEFRQQVLQKFTEHKALDFMFDVVK
                     QSSLQAAWGASV"
     gene            1297..2010
                     /locus_tag="PK856_08725"
     CDS             1297..2010
                     /locus_tag="PK856_08725"
                     /inference="COORDINATES: protein motif:HMM:NF037707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRL02489.1"
                     /translation="MMVTFVSQCEKRAIKRTQRVLDAFVDRIGDRTWQTVMTEEGLLA
                     VRKLLRKTATKNTAVSCHWLRSRSRSDLVWIVGNRQQFDAQGRVAVNHTEKSFQNSAW
                     ENNWYYLPLIKALAALAALLHDWGKANAVFQAKLTQPNKLGDPLRHEWVSCLLLQALV
                     TQASADTAQDASWLQCLETWQWNEQGLQAALTAQAQGKSKLNALPPAAQLLAWLIVSH
                     HRLPDLKPVQGEAKHQGYA"
     gene            2011..>3055
                     /locus_tag="PK856_08730"
     CDS             2011..>3055
                     /locus_tag="PK856_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated helicase Cas3"
                     /protein_id="HRL02490.1"
                     /translation="MQCADLNALFKRLTQEWGYHNLEEGKPQARFPQCFAFEQGLLST
                     SEPWKKQVQKWAGRLQVALPLAEQAMQSGAWRVVLHHARLCLMLGDHHFSSLEQNQNW
                     QSPLALFANTGKDKQLKQKLDEHLCGVAGQALTISQYLQRFESEMDVAHDVRSLKQKS
                     PAQFAWQDKAVQGIQQFRAQHNDGMARGWFVVNMASTGQGKTIANAKIMRALSDDANS
                     LRYVLALGLRTLTLQTGDAYRDSLGMGDDELAVLIGSKAVLELHQLNVNQQKAQKTQQ
                     EEIQDAWAEHGSESAESLLDEDLRYAATDLPEFMQAVFKDNQAAKNQAFLFKSVLVCT
                     IDHMMAATETVRGG"
BASE COUNT          795 a          626 c          836 g          798 t
ORIGIN      
        1 gcatttctaa gctgcctgtg cggcagtgaa cctgaggaag aagaaaatag catcaatgtg
       61 ttttttctaa gctgcctgtg cggcagtgaa ctgtggcttt atttgccaca agctagggta
      121 gcaaacaatt tgcgggattt gaatcggaaa aacctttatt tttaatgctt ttaaaaattt
      181 aaaataaatc aatattttat aaatttcaaa aaactttggg taaattatgg gtatgaaaat
      241 ggtggcaagg tatttacagg tcgcatgtat tttaatttaa gccatatctt tttttgaata
      301 acttcactat tatgacattc aacttaaatg ttgagagctg gcatggaagt attgaccgat
      361 ttaaaagcca ttttgcattc gaaacgggcg aatatttatt atttggaaca ttgccgtgtg
      421 atgcaaaaag atggtcgggt tttgtatctt accgagtcta aaaagcaaaa tttctattgg
      481 aacatcccta ttgccaatac cactgtcatc atgctgggca cggggacatc gattacccaa
      541 gcggcgatgc ggatgttggc cagtgcaggg gtgttggtcg gctttttgtg gcggtggcgg
      601 tacgcctttg tttgcaggtg ctgaaattga atggttaacg cctcaaagtg aatacaggcc
      661 gactgagtat gtgcaaggct ggttgtcgtt ttggtttgat gaggccaagc gcttagaggt
      721 ggccaaatct ttgcagttgc agcgcttggc atttttacag caggtgtggc gcaaagacaa
      781 agatttgcaa caagcaggtt ttggcagtga agatgctgtg ttgcagcgtg gttttcaaag
      841 ttttgaggcg gccatcgctc gcagtcaaaa agtgggcgat ttgttgctca atgaggctgc
      901 gcttaccaaa aagctgtatc actttgcggc gcaaacctgt cgcattcaag atttcaatcg
      961 ctcgcatgaa aacggcgatt tggccaacca atttctctcg catggcaatt accttgccta
     1021 tggtctcgcc gcctgtactt tatgggtttt aggtattcca cacggatttg ctgtgatgca
     1081 tggcaaaacc cgtcgcgggg ctttggtgtt tgatgtggcc gatttggtga aagatgcgat
     1141 tgtgctgcct atggcgttta tgtgcgccag tgaaaatgcc agtgaacaag agtttcggca
     1201 acaggtactg caaaagttca ctgagcataa ggccttggat ttcatgtttg atgtggtgaa
     1261 gcaatcaagt ttacaggccg cttggggtgc atcggtatga tggtcacctt tgtcagtcaa
     1321 tgcgaaaagc gtgcgattaa acgcacgcaa cgggtattgg atgcgtttgt cgatcgcata
     1381 ggtgacagaa cgtggcaaac ggtgatgacg gaagagggtt tgttggctgt gcgcaaattg
     1441 ttgcgcaaaa ccgccaccaa aaataccgcc gtgtcttgtc attggttgcg ttcgcgcagc
     1501 cgcagtgatt tggtgtggat tgttggcaat cggcagcaat ttgatgcaca agggcgggtg
     1561 gcggtgaatc acaccgaaaa atcatttcaa aacagtgctt gggaaaacaa ttggtattac
     1621 ttgcccttga ttaaggcctt ggcggctttg gccgcgttgt tacacgattg gggcaaggcc
     1681 aatgcggtgt ttcaagccaa attgacgcag cccaataagc tgggtgatcc attgcggcat
     1741 gaatgggtgt cgtgtttgtt gctgcaagct ttggtcacgc aggcatctgc ggatacagcg
     1801 caagatgcaa gctggctaca gtgtttggaa acttggcagt ggaatgaaca gggcttacag
     1861 gccgctttaa cggcacaagc gcaaggcaaa agcaaattga atgcattgcc gccagcggcg
     1921 caattgctgg catggctaat cgtaagccac caccgattgc ccgatttgaa acccgtgcaa
     1981 ggtgaggcca aacaccaagg ttatgcgtaa ttgcagtgtg cggatttaaa cgctttgttc
     2041 aagcgtttga ctcaggaatg gggctatcac aatctggaag agggcaagcc acaagcgcgt
     2101 tttcctcagt gttttgcttt tgagcagggc ttgttgtcca cttctgagcc gtggaaaaaa
     2161 caggtgcaaa aatgggcagg gcgtttacag gtcgcattgc cattagccga gcaggcgatg
     2221 caaagcggcg cgtggcgtgt ggtgttacac catgcccgct tgtgcttgat gctgggcgac
     2281 catcactttt cttccttaga acaaaatcaa aattggcaaa gcccattggc tttgtttgcc
     2341 aacacaggca aagacaaaca actcaagcaa aaattggatg agcatttgtg tggtgtggca
     2401 ggacaggctt taaccatcag ccaatatttg cagcgctttg aaagcgaaat ggatgttgcg
     2461 cacgatgtgc gcagcttgaa gcaaaaaagc cccgcccagt ttgcatggca agacaaagct
     2521 gtacagggca ttcaacaatt cagggcgcaa cacaacgatg gcatggcgcg tggctggttt
     2581 gtggtgaaca tggccagcac aggccaaggc aaaaccattg ccaatgccaa aatcatgcgg
     2641 gctttgtctg atgatgcaaa ctcattgcgc tatgtgttgg ccttgggcct gcgcacgctc
     2701 actttgcaaa ccggcgatgc ttatcgcgac agcttgggca tgggtgacga tgaattggcg
     2761 gtgttgattg gttctaaagc cgtgttggaa ttacaccaac taaatgtgaa tcagcaaaaa
     2821 gcacaaaaaa cccagcaaga agaaatacaa gacgcatggg ccgagcatgg cagtgagtcg
     2881 gcagagtcgt tgttggatga agatttaagg tatgccgcta ccgacttgcc tgaattcatg
     2941 caagccgttt tcaaagacaa tcaggcagcg aaaaaccaag catttttgtt taagtccgtg
     3001 ttggtttgca ccatagacca catgatggcg gccacagaaa ccgtgcgcgg tggca
//
LOCUS       DAOMVK010000271         3050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_9476_length_3050_cov_3.505175, whole genome shotgun sequence.
ACCESSION   DAOMVK010000271 DAOMVK010000000
VERSION     DAOMVK010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9476_length_3050_cov_3.505175"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PK856_08735"
     CDS             complement(<1..204)
                     /locus_tag="PK856_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02491.1"
                     /translation="MALWIQQPLPHILNVVVMFLWLLLSIAVVLSRFKTLHFRHKTLK
                     AAYFTCFALGLVWYFNIPAKQDRD"
     gene            complement(415..807)
                     /locus_tag="PK856_08740"
     CDS             complement(415..807)
                     /locus_tag="PK856_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02492.1"
                     /translation="MDNIYEWLGISADATETEILAAIAAKKQNYQLNTSQEVHIKQVL
                     LTPHLRKHHDLRMATAGHGAGFSGGAFAPMDLGIDDTASYTPAENHTVIRTKLKKRKT
                     HPSNIMYLCLAIAVLAFLLNLLFKLNAI"
     gene            1099..1854
                     /locus_tag="PK856_08745"
     CDS             1099..1854
                     /locus_tag="PK856_08745"
                     /inference="COORDINATES: protein motif:HMM:NF023166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HRL02493.1"
                     /translation="MARTFFLLILASAVPVLWAKTSPLQTTEHSLTKKSELICIHEDQ
                     QKTCSLYTINGLQTNWPWLSQRIRKQSESEQPVLTAKRLPVPTGFKTKSDWVMQREYA
                     VKHTGTYRDLVGIEFGGWDYGNGAAHGNPYASHNLYDAQTQQEVSFADMVLPEKKEAL
                     KQALQQSFATWLNREQDDDKGRDAAEEQDDAEEYWPEHAKISDNVTLSKQGVVFHYGV
                     YELGAYAMGTPDLELTWKQVLPMLKPQWRQRFK"
     gene            complement(1921..>3050)
                     /locus_tag="PK856_08750"
     CDS             complement(1921..>3050)
                     /locus_tag="PK856_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HRL02494.1"
                     /translation="NFSKYFGDLKTGVNFKQPELAKTLGRISQNGADEFYRGETAKLL
                     VAQMQRDGGLISAEDLNKYKVKWRKPMSIKWRDNVIHTAPLPSSGGIALRQLLAMKEY
                     RAADFKGVPLNSAQYMHLVSEIEKRVFADRADYLGDPDYTKAPVKKLTSDVYLQKRAA
                     EVNAKTISPTEHVKPGLEPAHTTHFSILDKDGNAVANTYTLNWDYGNGVVVEGAGFLL
                     NDEMDDFSAKAGTANIFGVVGSDANAIEPGKRMLSSMSPTIMTQDGKVNLVIGTPGGS
                     RIFTSIFQVINNLYDYNLPLQQAVDAQRFHHQLLPKDVIYYDDFAPIKGDVAAQLTAM
                     GYKVEDQGWRMGDIQAIQVKDGKVAAASDSRGRGHHAVIQP"
BASE COUNT          819 a          685 c          761 g          785 t
ORIGIN      
        1 gtctctgtct tgtttggctg ggatgttgaa ataccaaacc aagccgagcg caaaacaggt
       61 gaaataggcc gctttgaggg ttttgtgcct gaaatgcaga gttttaaaac ggcttaatac
      121 gaccgcaatc gacagcaaaa gccacaaaaa catcacgacc acattcaaaa tgtggggcaa
      181 aggctgctga atccacaacg ccatggctaa ccacatggtt atcaacagat aacacaatac
      241 tgccatcgaa tgcttcaaga cagtggcgat tcttactgcg caagcgctaa tgctgggcat
      301 cataagtttt cattcaagat taaagaagca gtaggcatgg actgtaaaac agcaccgcca
      361 aatgctcaag cggcctgtaa acatttacag gccgcttgag agcacagatt caaatcaaat
      421 agcattcaat ttaaacaaca aattcaacaa aaatgccaat acagcaatgg ccaagcacaa
      481 atacatgatg ttgcttgggt gtgttttgcg ctttttcagt ttggtgcgta tcacggtgtg
      541 attttcggca ggagtgtaag aagcagtatc gtctatgccc aaatccatgg gtgcgaaggc
      601 accacctgaa aatcctgcgc catggcctgc agttgccatg cgcaaatcat ggtgtttgcg
      661 cagatgaggc gtgagcaaaa cctgtttgat gtgtacctct tgagaggtat tcaattggta
      721 gttttgcttt ttggcagcaa tggcagccaa gatttcggtt tctgtggcat cggctgagat
      781 gcccaaccat tcataaatat tgtccatgtg acagtccttc aatgtgacaa caaaagtgat
      841 ggatggaaat gtgtggttgt catcactttg gggtatatcg gtttggcttt attatgatgt
      901 gaatttaaca ctgaaaaatg tatagaaagc acagatttaa gttaattttt ataaataagc
      961 tctaagcttt aattgcggta agtggcacag ttcaatacct gacagtgcat attgaatggc
     1021 gacaacattg cgcattgcct cgcagaggct aactcattat gctaaaagta ttcaatgcaa
     1081 aagtgaaagc gaaccatcat ggcacgtact tttttcctct tgattttggc cagtgccgtc
     1141 cccgtgttgt gggcgaaaac gtcaccatta caaaccacag aacacagctt gaccaaaaaa
     1201 agcgagttga tttgcataca tgaagatcaa caaaagactt gttctttgta taccattaat
     1261 ggcttgcaga ccaattggcc gtggctgagc caacgcatac gcaaacaatc agagtcggag
     1321 caacccgtgc tcacagccaa acgtttgcct gtacccacgg gcttcaaaac gaaaagtgat
     1381 tgggtaatgc agcgtgagta tgcggtaaaa cacacgggta cttaccgtga tttggttggc
     1441 atcgaatttg gcggttggga ttatggtaat ggtgccgcgc acggcaatcc atacgccagc
     1501 cacaatttat acgatgctca aacccaacaa gaggtgagtt ttgccgacat ggtattgcct
     1561 gagaagaaag aagcgttgaa gcaagccttg cagcagagtt ttgcaacatg gttgaaccga
     1621 gaacaagatg atgacaaagg acgagatgct gcagaagaac aagatgatgc tgaagagtat
     1681 tggcctgaac atgccaaaat ttccgacaac gtgacactca gcaagcaagg cgtggtgttt
     1741 cactatggtg tgtatgagct gggtgcgtat gcgatgggta cgccagattt agagctgact
     1801 tggaagcaag tcttgcccat gttgaagccg caatggcgac aacgctttaa ataaatggcg
     1861 gtaaacaaat aagcggcctg taaatgttta caggccgcta ttcaatggtt caaaccgatt
     1921 ttaaggctgt atcaccgcat ggtgtccacg gccacggcta tcggaggccg cagcgacttt
     1981 gccgtctttc acttgtattg cttggatgtc gcccatgcgc cagccttggt cttcgacttt
     2041 gtagcccatg gccgtgagtt gggcggccac gtcaccctta atcggcgcga aatcatcgta
     2101 gtaaatcacg tcttttggca gcaattggtg gtgaaaacgc tgtgcgtcta ccgcttgttg
     2161 caaaggcaag ttgtaatcgt acaaattgtt gattacttgg aagatggagg tgaagatgcg
     2221 cgagccaccc ggtgtgccga tgaccaaatt cactttgcca tcttgcgtca tgatggtcgg
     2281 gctcatggac gagagcatgc gcttgccagg ctcaatcgca ttggcatccg aaccgaccac
     2341 accgaagatg ttggccgtgc ctgctttggc actgaaatcg tccatttcat cgttgagcaa
     2401 gaagcccgcg ccttctacca ccacgccatt gccatagtcc caattcaagg tataggtgtt
     2461 ggccaccgca ttgccgtctt tgtctaatat tgaaaagtgg gtggtgtggg caggctctaa
     2521 gcctggcttc acgtgttcag tgggcgaaat cgttttggca ttcacttcgg cggcgcgttt
     2581 ttgcaaatac acatcgctgg tgagcttttt cacgggggct ttagtgtaat cggggtcgcc
     2641 caaatagtcg gcacggtcgg caaacacgcg cttttcaatt tcggatacca agtgcatgta
     2701 ttgagcagaa ttgagtggca cgcctttaaa atcggcggcg cggtattctt tcatcgccaa
     2761 caattgacgc aaggcgatgc cgccagagct gggcaatggc gcggtgtgga tgacattgtc
     2821 gcgccattta atgctcattg gtttgcgcca tttcaccttg tatttgttca aatcttcggc
     2881 actgatcaac ccaccatcac gctgcatttg tgccaccagt aatttggcgg tttcgccgcg
     2941 gtaaaattca tctgcgccat tttgtgaaat gcggcccaaa gtcttggcca actcaggttg
     3001 tttgaaattc acgcctgttt tcaaatcgcc aaaatacttg gagaaattgg
//
LOCUS       DAOMVK010000272         3049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_9480_length_3049_cov_2.094856, whole genome shotgun sequence.
ACCESSION   DAOMVK010000272 DAOMVK010000000
VERSION     DAOMVK010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9480_length_3049_cov_2.094856"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="PK856_08755"
     CDS             complement(<1..1197)
                     /locus_tag="PK856_08755"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HRL02495.1"
                     /translation="MSDQLARYPEYNQIIQAVLKADALLSGQKEVLELLAKRAPLFQV
                     LHRLVSIVEEQYDGTFCSILLVDSVNKTFKAGVRVEYEKNYSEEETGVSILPPYMGPC
                     CMASHLHEPVLVADIANDARWQAPWREWALSNDLQSCRSQPIYSSTGEVLGTFAMYHK
                     KYADPTSANLYQIEVTSHIAGIAIERKLIEQAELLKIEDERRLNAELTQAIQMRDEFL
                     SIASHELSSPLALLKMQTELHKEMLEEGELTHDDMVQLFDNHAARLDKVIAAVRTMLD
                     SSSASVGKLELNYHDFDLSELLNDVVQRLKPLVEKSGTHIQLQANTPILGQWDQFKIE
                     QVLTNLITNSIKYGDHKPIHISLSTEQGIALLEVQDQGIGIAPEDHSKIFERFERVVP
                     TGNKASG"
     gene            complement(1534..1935)
                     /locus_tag="PK856_08760"
     CDS             complement(1534..1935)
                     /locus_tag="PK856_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HRL02496.1"
                     /translation="MQTKAILGEQEIQTMLQAATAKAKENQWQVAIAIVDDGGHLLGF
                     SRLDGCAPVAAHIALEKAKSAALGRRETKGYEDMINSGRTAFVSAPLQGLLEGGVPVV
                     IKGQVIGAVGVSGLAKELDAELAKAAVNAII"
     gene            complement(2028..2453)
                     /locus_tag="PK856_08765"
     CDS             complement(2028..2453)
                     /locus_tag="PK856_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02497.1"
                     /translation="MKTFWSLSINLSCILALMACSPTSTEIVEPTSVPALDEQAFKTQ
                     FDQNFQQSCFTGIQSQFETEQISANEDDLQAAQEVCACISYNLIQNNTVEQLQSLEQM
                     SASAAQETTQPLVNSCQEQIQLQSESASSPHAASTSHEN"
     gene            complement(2455..>3049)
                     /locus_tag="PK856_08770"
     CDS             complement(2455..>3049)
                     /locus_tag="PK856_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HRL02498.1"
                     /translation="LHNQMHDDGLNDMRRNIDAWWPAIEAGAEAILINASGCGSTVKE
                     YGFHLQNDAQYAQKAQRVSELAKDIVEILIGETEGLKALFAHAPSAATLAYHPPCSLQ
                     HGQKLGGKVETLLSGLGYPVLLPKDSHLCCGSAGTYSFLQPELSGRLLQNKVNNLEAL
                     QPKAVLSGNIGCIAHIGSGTDIPVMHWIEYLDQQLGN"
BASE COUNT          742 a          645 c          735 g          927 t
ORIGIN      
        1 gccagatgct ttattgccag tagggacaac gcgctcaaag cgttcaaaaa tcttactgtg
       61 gtcttcaggt gcaatgccta tgccttggtc ttgcacttcc aagagtgcta tgccttgctc
      121 ggttgagagt gagatgtgta tgggtttgtg gtcgccatat ttgatgctgt tggtaatcaa
      181 attggtcaat acctgttcta ttttgaattg gtcccattgg ccgagtatgg gggtgttggc
      241 ttgtaattgt atgtgtgtgc ccgatttttc caccagtggc ttcaggcgct gtacgacatc
      301 attgagcaat tcgctcaagt caaaatcgtg gtaattcaat tccagtttgc ccacggaggc
      361 gctggacgaa tccaacatgg tgcgcacagc ggcgatgact ttgtctaaac gcgcagcatg
      421 gttgtcaaac aattgcacca tgtcatcatg ggtcaactcg ccttcttcca gcatttcttt
      481 gtgaagttca gtttgcattt tcagcaaagc cagtggggag ctgagttcat gggatgcaat
      541 agagagaaat tcatcacgca tttgtatggc ttgagtcaac tctgcgttca aacggcgctc
      601 atcttcaatt ttcaacaatt cggcttgttc aatcagtttg cgctcaatgg caataccggc
      661 aatgtgtgaa gtcacttcga tttggtacag attggccgaa gtggggtcag catatttttt
      721 gtgatacatc gcaaacgtgc ccaaaacctc acctgttgac gagtaaatgg gttgtgagcg
      781 gcaagactgt aaatcattgc ttaaagccca ttcgcgccaa ggtgcttgcc aacgggcatc
      841 attggcaatg tctgccacca aaacaggttc gtgcagatga gaagccatgc agcatgggcc
      901 catgtatggc ggcaatattg aaacccctgt ttcttcttcg ctgtagtttt tttcgtactc
      961 tacgcgcaca cctgctttaa aggttttgtt gacgctgtca accaatagta tggagcaaaa
     1021 agtgccgtcg tattgttctt caacgataga taccaatctg tgcagcactt ggaaaagtgg
     1081 cgctcttttt gctaggagtt ctaagacttc tttttgtcca gacaaaagtg cgtctgcttt
     1141 aagcactgct tgaatgattt ggttgtattc tggatagcga gcgagttggt ctgacatggt
     1201 atccacattc tgtctaatct attcgaatgt aatattatga agatgagaag ggctgtttgg
     1261 ataccattat ttatggaaaa tggcaaaaaa tgtttatatg caaattaact gttttggcag
     1321 gcttcttgca tgtaaatatc agtatcaatt aagggttttg gctgattgag cttagagttt
     1381 aaaattaaaa tatctatcat tgttatgatg ttattttaat tttaaagttt gatgataaca
     1441 atgctgagat gaactctgag ttgagtttta gctaaatgcc caaatgaaat gcctcctgaa
     1501 acagaaggta ttttcatttg ggttgatatc atgttaaata atggcgttta ccgctgcctt
     1561 agccaattcg gcatccaatt ctttggccaa acctgacaca ccaactgcgc ctatcacctg
     1621 accttttatg acaactggga caccaccttc gagtaagcct tgtagcggcg cagatacaaa
     1681 tgcggtacga ccactgttaa tcatgtcctc ataacccttg gtttcacgac gacctaaggc
     1741 cgcagatttg gctttttcta aggcgatgtg ggcggcaact ggggcgcaac cgtctaagcg
     1801 gctgaagccc aataagtgac caccatcgtc cacgatggca atggctactt gccattggtt
     1861 ctctttggct ttggctgtgg cggcttgtaa catggtttga atttcttgtt cacctaaaat
     1921 ggctttggtt tgcataatgt gtatcctttg acagggtgga aagagaaagc gagtcggttc
     1981 aatggcgatg cgcttatgtt atttgtgtat gacattgaca aattttgtca gttttcatgg
     2041 ctggtgctgg cagcatgagg cgaagaagca gattctgatt gcaactgtat ttgttcctga
     2101 catgaattca ccagtggttg ggtggtttct tgggcggcac tcgcactcat ttgctccaag
     2161 ctttgcaatt gttccacagt gttgttctgt atcaaattgt aggagatgca ggcacacact
     2221 tcttgagcgg cctgtaaatc atcttcattg gcactgattt gttctgtttc aaattggctt
     2281 tgaatgcctg taaaacacga ttgttgaaag ttttgatcaa actgggtttt aaaagcttgt
     2341 tcgtccagtg ctggcactga agttggttcc actatttcgg tgctggttgg agaacaagcc
     2401 atcagcgcca atatgcacga cagattgata ctcagtgacc aaaatgtttt catgtcagtt
     2461 gcccaattgt tggtctaggt attcaatcca gtgcatcacg ggaatgtcgg tgccgctgcc
     2521 tatgtgggcg atgcaaccga tattgcctga caataccgct ttgggttgta aagcttctaa
     2581 attattcact ttgttttgca acaagcggcc tgaaagctct ggctgtaaga aagaatacgt
     2641 gcccgcagag ccgcagcaca aatggctgtc tttcggtaat aatactgggt agcccaagcc
     2701 tgacaacagg gtttccacct tgccgcccaa tttttgaccg tgttgcaaag aacaaggggg
     2761 atggtaggcc agtgtagctg cgcttggggc atgagcaaac aaggctttta agccttcggt
     2821 ttcgccgatt aaaatttcca caatgtcttt ggccaactcg ctcacgcgtt gggctttttg
     2881 cgcgtattgg gcatcgtttt gcaaatgaaa accgtattct ttaaccgtgc tgccgcagcc
     2941 tgaagcattg attaaaatcg cctctgcccc tgcttcaatc gcaggccacc aagcatcaat
     3001 gttgcgacgc atatcattta ggccgtcatc gtgcatttgg ttgtgcagg
//
LOCUS       DAOMVK010000273         3044 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_9500_length_3044_cov_2.875209, whole genome shotgun sequence.
ACCESSION   DAOMVK010000273 DAOMVK010000000
VERSION     DAOMVK010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9500_length_3044_cov_2.875209"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /gene="tilS"
                     /locus_tag="PK856_08775"
     CDS             complement(<1..445)
                     /gene="tilS"
                     /locus_tag="PK856_08775"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRL02499.1"
                     /translation="MLKNKLQQAILKTWSEQGSDHFHLEVGLSGGVDSVVLLHALKQL
                     QPELGFVLSAVHVNHQLQASATEWVQFCQNLCEAWEVSLRVEVVNVVHRQRLGVEAAA
                     RAARYQAYANTSADAVVLAHHADDQIETAFLGWLRGGPIPPHRLRY"
     gene            complement(432..1391)
                     /locus_tag="PK856_08780"
     CDS             complement(432..1391)
                     /locus_tag="PK856_08780"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HRL02500.1"
                     /translation="MKQVFLDFEQPIAELSQKIEELRFVQDDSVVDISEEIKRLQKKS
                     DDLTKTIFTKLTPVQISQVSRHAQRPYTLDYIEHIFTDFQELHGDRNFADDPAIVGGL
                     ARFNGQSVMVIGHQKGRDTKEKIYRNFGMPKPEGYRKALRLMKLAEKFHLPIITFIDT
                     PGAYPGIGAEERGQSEAIGLNLYEMSDLHVPVVCTVIGEGGSGGALAIAVGDYVNMLQ
                     YATYSVISPEGCASILWKTAEKAPEAAQALGITAKRLHELDLIDKVIPEPLGGAHRNF
                     VEIMQNVKETITEQLRQAQDLPMSDLLTRRFDRIMAYGTYAEK"
     gene            1621..2010
                     /locus_tag="PK856_08785"
     CDS             1621..2010
                     /locus_tag="PK856_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HRL02501.1"
                     /translation="MSEINTMRLDKWLWAARFFKTRALAQKHIELGRIKVNNERVKNS
                     KYIVVGDVVDMSLNSLPFRLTVNGLNMQRRPAPEARLLYVEDADTIAKREHIKLLNQA
                     ADMQTDYPEGRPTKRDRRQLDRLKQDW"
     gene            complement(2229..2537)
                     /locus_tag="PK856_08790"
     CDS             complement(2229..2537)
                     /locus_tag="PK856_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02502.1"
                     /translation="MNGSRLYVDFNELIDEDLVLLSQTDVTLDQQGNEVFLYPGKHVD
                     IYTDDIDDNGCVDNLIASGVVELNDPGVFPVCKWNCRINENGIQHESDLKIAQKYNEE
                     "
     gene            complement(2789..>3044)
                     /locus_tag="PK856_08795"
     CDS             complement(2789..>3044)
                     /locus_tag="PK856_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003780365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HRL02503.1"
                     /translation="RIDGLEDESRAGVATALAVANLPQVYIPGKSGLAIATGVHKGEV
                     GYALGYSSISEGGNWVFKASATANRMGDVGGGAGLLYMFD"
BASE COUNT          867 a          782 c          596 g          799 t
ORIGIN      
        1 aatacctcag ccgatgcggt ggtattggcc caccacgcaa ccatcctaag aaagccgttt
       61 caatttgatc atcggcatgg tgggccaata ccaccgcatc ggctgaggta ttggcatatg
      121 cttgataccg agccgctctc gctgcagcct ccacgcccaa tcgctgtcgg tgaaccacat
      181 tgaccacttc aacgcgcaaa gacacttccc atgcctcaca caagttttgg caaaattgaa
      241 cccattccgt tgcagatgct tgcaattgat ggtttacatg cacagcagaa agcacaaaac
      301 ccagttctgg ctgcaattgc tttaaggcat gcaataacac cacagagtcc acaccgccac
      361 tgagtccaac ttccaaatga aaatggtcag aaccctgttc tgaccacgtt tttaaaatag
      421 cctgttgcag cttatttttc agcatacgtt ccataagcca tgatgcgatc aaagcgacgt
      481 gttaacaaat ccgacatcgg caagtcttgc gcttgacgca attgttcggt gatggtttct
      541 ttcacatttt gcatgatttc cacaaaattt ctgtgcgcgc cacccaaagg ttctgggatg
      601 actttatcaa tcaagtccaa ttcatgcaaa cgcttggcag taatgcccaa tgcttgtgcc
      661 gcttcagggg ctttttctgc ggttttccac aaaatcgagg cacaaccttc tggagaaatc
      721 actgagtaag tcgcgtattg caacatgttc acataatcgc ccacggcaat ggccaaagca
      781 ccaccggagc cgccttcacc tatcaccgtg cacaccacag gcacatgcaa atcactcatc
      841 tcatacaaat tcagaccaat cgcctctgat tgaccgcgtt cttcagcacc aatcccaggg
      901 taagcacctg gggtatcaat aaaggtgatg atgggcaaat gaaacttttc agccaatttc
      961 atcaaacgca aggctttgcg atagccctca ggtttgggca taccaaaatt gcggtaaatt
     1021 ttttctttgg tgtcacgacc tttttgatgg cctatcacca ttaccgattg accattgaaa
     1081 cgggccaaac cacccacaat ggcaggatcg tcggcaaagt tgcggtcacc atgcagctct
     1141 tgaaaatcgg taaagatgtg ttcgatgtag tccagcgtat aaggacgttg cgcatgacgc
     1201 gaaacctgtg aaatctgaac gggcgtgagt tttgtgaaaa tggttttggt taaatcgtcg
     1261 cttttctttt gtaagcgttt gatttcttca gaaatatcaa ctacagagtc atcctgaaca
     1321 aaacgcaact cttcaatttt ttggcttaat tctgcaatcg gttgctcaaa atccaaaaat
     1381 acttgcttca tgcagtggta tccatatgtc gtaaattatc aattatcata cgctatcttc
     1441 cccaaagacg cagctttttt gcgccttgcc tgatggcgta tccatgaaaa ttggggtaaa
     1501 aattcggcct tcattatggc ataggcctgc cttaattcaa acactaaaaa acaggcttgc
     1561 caaacacaag ccctgtcttt atcagtacaa taacacactt atgttatggg aaaaccgatt
     1621 atgagtgaaa tcaacaccat gcgtttggac aaatggcttt gggccgcccg tttctttaaa
     1681 acccgcgctc tggcgcaaaa acacattgaa ttgggccgca tcaaagtcaa caatgaacgc
     1741 gtcaaaaaca gcaaatacat cgtggtgggt gatgtggtgg acatgtcttt aaacagtttg
     1801 ccgtttagac tgactgtgaa tggtttgaac atgcagcgtc gtcctgcacc tgaagcgcgt
     1861 ttattgtatg tagaagatgc ggacactatt gccaaacgtg agcacatcaa actcctcaac
     1921 caagccgccg acatgcaaac cgattaccct gagggacgcc caaccaaacg cgaccgccgt
     1981 caactggaca gattaaaaca agattggtaa gactcaggcc actcacccat ttgctcactc
     2041 ttctctatta acaaataaag cggcctgtaa acccaaatat cagcacgaaa aggtttacag
     2101 gccgctgtta catgtgattc tgtcttcaat tcaaccccgt tttgaaaaca cgggttttta
     2161 tttgccaaat aacccttccc ttacatacac aatcaatgcg ccagagcgac aacggttaat
     2221 tatgcggttt attcttcatt gtatttctgc gcgattttta aatcactttc atgctgaatg
     2281 ccattttcat tgattctgca attccactta cacacgggaa acaccccagg atcatttagt
     2341 tccaccacac cactggcaat taagttgtct acacaaccat tgtcgtcaat gtcatccgta
     2401 taaatgtcta cgtgtttgcc tggatacaag aatacttcat tgccttgttg gtcaagtgtg
     2461 acatcggttt gtgaaagcaa aaccaaatcc tcatcaatga gttcgttaaa atccacatac
     2521 aatctggaac cattcatttg aatacctcac tgatttgact gttttaaaat aaatggtgaa
     2581 ctgaaaattt ggatagaaaa ctggactatt catccattca cagcattcaa caccaaatta
     2641 acaaaataca agcctccact ctacatggct ttgtatttgc agcaccagag ctgctttggt
     2701 gtacatttaa tgtataaata ccatcactgt aacaaaaaca gcagccatct attgcgatga
     2761 ctgctgtgtg ttttacaggc cgcttgtatc aatcaaacat atacaacaag ccagcaccgc
     2821 cgcccacatc gcccatgcga ttggcagtgg ccgaagcctt gaatacccag ttaccacctt
     2881 cagaaatcga cgagtaaccc aaggcataac cgacttcgcc tttgtgtacg ccagtcgcaa
     2941 tcgccaaacc gctcttacct gggatgtaga cttgcggcaa gttggccaca gccaaggccg
     3001 ttgccacgcc agcccgcgac tcgtcttcca aaccatcaat gcgg
//
LOCUS       DAOMVK010000274         3027 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_9555_length_3027_cov_3.293405, whole genome shotgun sequence.
ACCESSION   DAOMVK010000274 DAOMVK010000000
VERSION     DAOMVK010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9555_length_3027_cov_3.293405"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="PK856_08800"
     CDS             <1..378
                     /locus_tag="PK856_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="1-aminocyclopropane-1-carboxylate
                     deaminase/D-cysteine desulfhydrase"
                     /protein_id="HRL02504.1"
                     /translation="AVGTGGTLAGLINASAAQQRLIGYAALKTDHLSAEIMHMTHKRQ
                     WQVFSEEVFGGYGRFNAELLDFIANTQAHNDLPLDPIYTGKAFYRLSQHIAEGLFAPN
                     SRILFIHTGGLQGHRPVSPEHAG"
     gene            519..821
                     /locus_tag="PK856_08805"
     CDS             519..821
                     /locus_tag="PK856_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02505.1"
                     /translation="MLDALFHNRTAHLALVLITLLLSFVLALLAVFGSIYQSIAYLVF
                     LPVCFMFFLSGLFGLLLAVDEPCEQPRLKRLYSLQLAVLLFAVLLIPLSATFLTEF"
     gene            823..1476
                     /gene="hisG"
                     /locus_tag="PK856_08810"
     CDS             823..1476
                     /gene="hisG"
                     /locus_tag="PK856_08810"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRL02506.1"
                     /translation="MTASHNLTIALSKGRIFDETLPLLAAAGIAPAEDPEKSRKLIID
                     TNHPNIRLVIVRATDVPTYVQYGAADLGIAGRDVLTEYGGDGLYQPLDLRIAKCRMMV
                     AVKKGFDYEAASRPGSRLKVATKYPDIAMEHFSNKGVHVDIIKLYGSMELAPLVGLSD
                     AIVDLVSTGNTLKANNLEAVEHISDVSSRLVVNKAALKLKYEVIQPIIDAFRDARQD"
     gene            1710..2045
                     /locus_tag="PK856_08815"
     CDS             1710..2045
                     /locus_tag="PK856_08815"
                     /inference="COORDINATES: protein motif:HMM:NF018684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HRL02507.1"
                     /translation="MKKSLLMIATAASLLAVSFSSQAASFNCKKASKPTERAICANQR
                     LNESDTKLTNTYNILLRFGGDNRRIRNDQLQWLRDRNQCGNRVACISEEYRYRQQQLE
                     QEIGQYQGG"
     gene            complement(2193..2522)
                     /locus_tag="PK856_08820"
     CDS             complement(2193..2522)
                     /locus_tag="PK856_08820"
                     /inference="COORDINATES: protein motif:HMM:NF018684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HRL02508.1"
                     /translation="MFKQWPTVFVMVSVMLLPLASQAASFNCKKASKPTERAICHNSQ
                     LSQADEAMNETYQILMGYDGDKRYIRNEQLRWLRERNQCGSRVACIGKKYEQRIQALQ
                     FQVEQYH"
     gene            complement(2564..2974)
                     /locus_tag="PK856_08825"
     CDS             complement(2564..2974)
                     /locus_tag="PK856_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRL02509.1"
                     /translation="MTYQAHAKSTKQKYIVDLSNEAGHVWHVDEPVAKGGGDTAPNPM
                     EVVLSALGACTTVTLQMYADHKGWDLQSVKVDLVLDPEEPKAGEARQIVRQVQVTGNL
                     DEAQKERLLKVAESCPVHKLLAGEISIQTALQAL"
BASE COUNT          716 a          796 c          717 g          798 t
ORIGIN      
        1 gctgttggca cgggcggcac gcttgctggc ctcatcaatg caagcgctgc ccagcaacgc
       61 ctcattggct atgctgcgct gaaaaccgac catctcagcg ccgaaatcat gcacatgacg
      121 cacaagcggc agtggcaggt tttttcagaa gaggtgtttg gcggttatgg ccgtttcaat
      181 gccgaattgc tggatttcat cgccaacaca caagcgcaca atgacttgcc actcgaccca
      241 atctacactg gcaaagcgtt ttaccgcttg agccaacaca ttgcagaggg cttgtttgcg
      301 ccgaacagcc gcatcttgtt catccacaca ggcggcttgc aaggccatcg gcctgtcagt
      361 cctgaacatg caggctgata cccattggtg ctgcgttgat gggcacacaa atccgcactc
      421 atcttggcat ctctgatgta agtcaataca ccgttttcgc cgcagggtat aaaaactttg
      481 ttaaaatgcc ccagcaagct gggcacaagg agcgaaaaat gttagacgca ttgtttcaca
      541 accgcacagc gcatttggct ttggtattga ttaccttgtt gttgtcgttt gtattggctt
      601 tgttggccgt gtttggcagc atttaccaaa gtattgctta tcttgtgttt ttacctgtgt
      661 gcttcatgtt ttttttatcg ggtttatttg ggctgttgct ggctgtcgac gaaccttgcg
      721 agcagcctcg cctgaaaaga ctctactcgc tgcaattggc ggtattgctg tttgccgtgt
      781 tattgatacc gttgtcggcg acttttttga cagaattctg agatgaccgc tagccacaat
      841 ttgaccatcg ccttgtccaa aggccgcatt tttgatgaaa ccctgccctt gctggccgcc
      901 gcaggcatcg cccctgctga agacccagaa aaatcccgca agctcatcat agacaccaac
      961 catcccaata ttcgcttggt gattgtgcgc gcgactgacg tgcccactta tgtgcaatac
     1021 ggcgcggccg atttgggcat tgcaggtcgc gatgtgctca ccgaatacgg cggcgacggc
     1081 ctgtatcaac ccttggattt gcgcattgcc aaatgccgca tgatggtggc ggtgaaaaaa
     1141 ggtttcgatt acgaggccgc atcacgtcca ggtagccgct tgaaagtggc caccaaatac
     1201 cctgacatcg ccatggagca tttctccaat aaaggcgtgc acgtggacat catcaagctg
     1261 tatggctcca tggagttggc tccgttggtg ggcttgtctg atgccatcgt cgatttggtg
     1321 tctacaggca acaccttgaa agccaacaat ctggaagcgg tggaacacat cagcgatgtg
     1381 tccagccgtt tggtggtgaa caaagccgca ttgaaactca aatacgaagt catccaaccg
     1441 attatcgatg ccttccgcga cgcgcgccaa gattaattgc acaaatcaaa gcggcctgta
     1501 aatctgaaac aaggtttaca ggccgctttt ttgttttcac cgttcaggat ttttattgaa
     1561 agaatggaac actgggacac attccaaact aagcagcgca gttgcttctg cttaaggtag
     1621 caagcgccgc acttttatgt gggccacttg tctgatggaa gcacttcgat tagtgttggc
     1681 ctgtgttgtc actttaaaag gaatccgcca tgaaaaaatc cctgttgatg attgccaccg
     1741 ccgcgagctt gttggcggta tcgttttcca gccaagccgc tagcttcaat tgcaaaaaag
     1801 ccagcaaacc cacagaacgg gccatttgcg ccaatcaacg cctgaatgaa tccgacacca
     1861 aactgaccaa cacttacaat attttattga ggtttggcgg tgacaaccgc cgcatccgca
     1921 acgatcaatt gcagtggttg cgcgaccgca atcagtgcgg caaccgtgtt gcttgcatca
     1981 gtgaagaata ccgctaccgc caacaacagt tggaacaaga aatcggccaa taccaaggcg
     2041 gttaatgccc tgccatgatt tgaatatcaa tgcggcctgt aaaccctctc tggatttaca
     2101 ggccgcttat ttgtgacttt tacttcaaaa tctcatgccg catggaatgc ttatcttaaa
     2161 ttgaggttca gccatggata tgggtaaaac gcttaatggt attgctccac ttgaaactgc
     2221 aatgcctgaa tcctttgttc atacttttta ccgatacaag caactcggct gccacactga
     2281 ttgcgctcac gcaaccaccg cagctgctca ttgcgtatgt agcgtttatc gccatcgtaa
     2341 cccatcagta tttgataggt ctcattcatc gcctcatcgg cttggctcaa ttggctgtta
     2401 tgacaaatcg ctcgttcagt gggtttgctg gcttttttgc aattgaagct cgcagcctga
     2461 ctggcaagcg gtaacagcat cacgctcacc atcacaaaga cggtaggcca ttgcttgaac
     2521 atattaggct ccttaaggac tgaaaacgaa tcaggtgttt cgtttacagc gcttgcaaag
     2581 cggtttggat gctgatttcg cccgccaaca gcttgtgcac aggacaagat tcagccactt
     2641 ttagcaaacg ctctttttgg gcctcatcca aattgccagt gacctgcact tggcgcacga
     2701 tttgacgtgc ctcacctgct ttgggttctt ctgggtcgag taccaaatcc actttgacgg
     2761 attgcaaatc ccaacctttg tggtcagcat acatttgcaa agtcaccgtg gtacacgcgc
     2821 ccaaagccga caacaccacc tccatcggat tcggcgcagt atcgccgccg cccttggcca
     2881 caggctcatc cacatgccaa acatggcctg cttcattact caaatccacg atgtattttt
     2941 gcttagtgct tttggcatgc gcttggtaag tcatggtgtc actccctttg gaatatggtc
     3001 ggtcatcggt ttatcgctgc caagtat
//
LOCUS       DAOMVK010000275         3020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_9584_length_3020_cov_3.249916, whole genome shotgun sequence.
ACCESSION   DAOMVK010000275 DAOMVK010000000
VERSION     DAOMVK010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9584_length_3020_cov_3.249916"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="PK856_08830"
     CDS             complement(<1..588)
                     /locus_tag="PK856_08830"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HRL02510.1"
                     /translation="MNNALQLNHIQAFAFDLDGTLVDSIKDLALAANAMREALGMEDL
                     PIDTLKSFVGDGMGRLVHRALTNDHEALADEALWQQGFAFFAQYYHEHIADYSSPYAG
                     VVDGVQLLKSLGYPVMVVTNKSERFAIKLLADLGLLDEFSLVIGGDTLPEKKPSALPL
                     QHVCDVLHIEPKQLAMVGDSHNDILTAKAAGAYAIG"
     assembly_gap    804..831
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1090..1395
                     /locus_tag="PK856_08835"
     CDS             1090..1395
                     /locus_tag="PK856_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02511.1"
                     /translation="MQTFKTTLTLALLLGLSAHMAQAKTKGVHNPDRVGQAAWVSSPP
                     TNIRATPNGKIQCVVQREERIRLYGSSGKGDHNGVWYYTDYCGKMGLIHSTQFVIFE"
     gene            1513..2004
                     /locus_tag="PK856_08840"
     CDS             1513..2004
                     /locus_tag="PK856_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02512.1"
                     /translation="MMQKQAVLTQLHTELGFELPSAYQAILLAFEQLYVGELVNGQDV
                     SFDALAHLLQPFTHNHQNHQRLQAFMADSAHKYPAGKVYRRDTDALMTTETFAQSIIM
                     GSGDDGVYWFWNKNDGAVWDYYLDDGSVSKAAQDFATWMTGFSLQICDEWNVFVDESD
                     GSE"
     gene            complement(2123..2614)
                     /locus_tag="PK856_08845"
     CDS             complement(2123..2614)
                     /locus_tag="PK856_08845"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRL02513.1"
                     /translation="MIKLHTNFGVIGIELDHEKAPITAANFEQYVKDGFYDGVIFHRV
                     IKGFMVQGGGMDADMKEKQTRDTIENEAANGLKNDKYTLAMARTSAPHSASAQFFINT
                     TNNDFLNFKAPNPQGFGYAVFGKVVEGFDVVDQIEGVKTGNKGFHQDVPKDAVVITKA
                     EIV"
     gene            complement(2635..>3020)
                     /locus_tag="PK856_08850"
     CDS             complement(2635..>3020)
                     /locus_tag="PK856_08850"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRL02514.1"
                     /translation="VFHRVIDGFMIQGGGMDAQMREKATDKAIVNEARNGLQNTVGTI
                     AMARTAAPNSATSQFFINTANNTPLNYKGPTPAGAGYAVFGRVTGGMDVVNKISKVRT
                     TSKGMHQDVPATPIVIKKVTVYQTK"
BASE COUNT          767 a          653 c          782 g          790 t
ORIGIN      
        1 gcctatggca taagcacctg cggctttggc ggtgagaatg tcattgtgtg aatcgccgac
       61 catcgccaat tgcttgggct ctatgtgtaa tacgtcgcac acatgttgca aaggcaaggc
      121 agatggcttt ttctcgggca aggtgtcgcc gccgatgacc aagctgaatt cgtctaacaa
      181 acccaaatct gccaataatt tgatggcaaa gcgttcggat ttgttggtga ccaccatcac
      241 agggtagccc aaagacttga gtagctgcac accatcgacc acaccagcgt aagggctgga
      301 ataatcggca atgtgttcat ggtaatactg ggcgaaaaag gcaaagcctt gttgccacag
      361 tgcttcatct gccaaagcct cgtggtcatt ggtgagcgcg cggtgtacca agcggcccat
      421 gccatcgcca acaaagcttt ttaaggtatc gataggcaaa tcttccatgc ccaaagcctc
      481 gcgcatggca ttggcagcca aggccaagtc tttaatcgaa tcgaccaaag tgccatccaa
      541 atcaaaagcg aaagcctgaa tgtggtttaa ttgcaaagcg ttattcatat cggtatgcgg
      601 cctgtaaaag tttaggaggc aattatacgg ccttataccc agaggtgcga atcttgcccc
      661 atggtggtgt caaacaggtc aaaacgacaa atgcgcttga taatggcatt gttgtcaaag
      721 aactgagttt aagaacaagg agaaagaaca tgaaatctac aggaattgta agaaaagttg
      781 acgagcttgg aagaatagtt cttnnnnnnn nnnnnnnnnn nnnnnnnnnn nattcgcgct
      841 acacccaatg gcaagatttt gtgtgtggcc aagaagcaag gcgctatcaa aatctatggt
      901 tctactggtg tgtatgatga caatggcgaa tggtattaca ccaattattg cggccgcatg
      961 ggcgtgatcc atggctcaca attcatttct ccaggttaat cacagcattg ggttggcatt
     1021 acatcggtgt gatagagatg ctgttgcgag caataataaa atcattctat ttttcagaga
     1081 aagtatccta tgcaaacttt caaaaccaca ttgaccttgg ccttgttgtt gggtttgagc
     1141 gcacacatgg cacaggcaaa aaccaaaggc gttcacaatc ctgatcgggt aggacaagct
     1201 gcttgggtgt ccagcccgcc caccaacatc cgcgccacgc ccaatggcaa aattcaatgt
     1261 gtggtgcaac gtgaggagcg cattcgtttg tatggatcca gcgggaaagg ggatcacaac
     1321 ggcgtgtggt attacaccga ctattgcggc aagatgggtt tgatccattc gacccaattt
     1381 gtgatctttg aataagtctg aataagcggc ctgtaaagct tacaggccgc ttagttgcag
     1441 ttttcgtttg aatggaatgt gaagctctgc gactgccatg cccagagata ttgggggtag
     1501 gaaatgatgg atatgatgca aaagcaagca gtgttgacac aattgcacac ggaactgggt
     1561 tttgaattgc ccagtgccta ccaagccatc ttgctggcgt ttgagcagtt gtatgtgggt
     1621 gagttggtca atgggcaaga cgtgagtttt gacgccttgg cgcatttgtt acagccattt
     1681 acgcacaacc atcaaaacca ccagcgtttg caagcattca tggctgactc cgcgcacaaa
     1741 tacccagcag ggaaagtgta tcgccgcgat accgatgcct tgatgaccac agaaacattt
     1801 gcccagtcca tcataatggg cagtggcgac gatggtgtgt attggttttg gaacaaaaac
     1861 gatggtgcgg tatgggacta ttatttggac gatggctcgg tgagtaaggc agcacaagat
     1921 tttgcaacat ggatgacagg gttttcactg caaatctgcg acgaatggaa tgtgtttgtg
     1981 gatgaaagcg atggaagtga ataaatcgtt ggtttagata atggtgatat agaacgaatg
     2041 ttgtttcata aacaagcggc ctgtaaacag ttacaggccg cttgtgattg tgtttaagcc
     2101 atcatcagct taaagccagt gcttaaacga tttccgcttt ggtaatcact accgcgtctt
     2161 taggcacgtc ttggtggaaa cctttgttgc cagttttcac gccttcgatt tggtctacca
     2221 cgtcaaagcc ttcaaccact ttgccgaaca cggcatagcc aaaaccttgt gggttaggag
     2281 ctttgaagtt caagaagtcg ttgttggtgg tgttgatgaa aaactgagcc gaagccgaat
     2341 gaggcgctga agtgcgcgcc atggcgaggg tgtatttgtc gtttttcaaa ccgttggccg
     2401 cttcgttttc gatggtgtcg cgggtttgtt tttctttcat gtcggcatcc atgccgccac
     2461 cttgaaccat gaagcctttg atcacgcggt ggaaaatcac gccatcgtaa aagccatctt
     2521 tcacgtattg ttcaaagttg gccgcagtga taggggcttt ttcgtggtct aattcaatgc
     2581 cgatcacgcc gaaattggtg tgtaatttaa tcatggtcag tctttcgcta atgattactt
     2641 ggtttgataa acagtgactt ttttaatcac aataggggta gcaggtacgt cttggtgcat
     2701 gcctttagaa gtggtgcgca ctttggaaat cttgtttacc acatccatgc cgccagtcac
     2761 ccgaccaaac accgcataac ccgctccagc tggggtcgga cccttgtaat tcaagggtgt
     2821 gttgttggca gtattgatga aaaattggct ggtggccgaa ttgggtgctg ctgtgcgcgc
     2881 catggcgatg gtgcctacgg tgttttgcaa gccgttgcgg gcttcgttga cgatggcttt
     2941 gtcggtggct ttctcacgca tttgtgcgtc catgccgccg ccttgaatca tgaagccatc
     3001 aatcacgcgg tgaaacacgg
//
LOCUS       DAOMVK010000276         3015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_9603_length_3015_cov_3.118243, whole genome shotgun sequence.
ACCESSION   DAOMVK010000276 DAOMVK010000000
VERSION     DAOMVK010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9603_length_3015_cov_3.118243"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="PK856_08855"
     CDS             <1..507
                     /locus_tag="PK856_08855"
                     /inference="COORDINATES: protein motif:HMM:NF037008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRL02515.1"
                     /translation="AVHYHYGNKEVLFLAVLKRRIAPFVNMVLDNMKQKAQLHASVRP
                     EDLVQAFVDVCLQYVRDFDREAVLVTRLVTRLMLDEYKVFREQLAQEYTDIIEHILHL
                     FEQALPHLDKETVRWRMHIALSTLFNSFAGNDVLKALARREWVNAKNPEQVARYVVPF
                     VVAGLVAP"
     gene            539..>3015
                     /locus_tag="PK856_08860"
     CDS             539..>3015
                     /locus_tag="PK856_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRL02516.1"
                     /translation="MLSLGLMLLLGGIAAYFRAKGWMIALAAFVGALIGGSVWWALLP
                     LVAGVVCCLPFVKSLYMPKVFAMFKKVTPPMSETEQDAINAGTVWWDGELFSGKPDYQ
                     KLLNYPHPQLTAEEQAFIDGPVEELCNMLNDWQINHELKDLPQEAWDFIKQQGFLGMI
                     IKKQYGGLEFSNYAHAKVVTKIATRCGSAAVTVMVPNSLGPGELLQHYGTEAQKDFYL
                     PRLAKGADIPCFALTSPSAGSDAGGIPDFGVVTRGSYTDPHTGERHEDVLGVRVSWEK
                     RWITLAPVATVLGLAFKMFDPDGLLGDKKEIGITCALVPTHHEGVQIGRRHWPSGSAF
                     MNGPTWGADVFIPLDWIIGGVDYAGRGWQMLVECLSVGRCISLPANSVAAGMVSSYTT
                     SLYARIRDQFGLPIGKFEGVDEALARIIANTYQMEASQDLALTGLDVGEKPSVISAML
                     KYHNTERMRKTLNDAMDIHGGRTVVQGPRNYLVPAYQAIPIAITVEGANILTRSLMIF
                     GQGAIRSHPYVLREMMAATNNDAASFNQVFGQHVHFVFTNVARSFWLGLTNGCLSKAP
                     QRGVTAPYYKQMNRVAANFALLTDMCMFSLGGSLKFREKLSARLGDIVANLYICSAVL
                     KRFERDGSQKADVAIMQYAAQQALFDAQVAMDGVLKNLPNRPLAWLLRGLTMPMGHAI
                     KAPSDTLGTQVANEAMKGEEALERLTHFMFKSSDESDAVGVLAPAHKAVIAAADAEKK
                     LRKLERDDQLDAPSPNLRLEQALQLSLISQAEFDLISKARNLKRQVIMVDDFDMQLEQ
                     HDDKLLERHMVIPPFLIELMGRKSKAL"
BASE COUNT          708 a          637 c          837 g          833 t
ORIGIN      
        1 gccgtgcatt accactatgg caataaagag gtcttgttct tggcggtatt gaagcgccgc
       61 atcgcgcctt ttgtgaacat ggtgctcgac aacatgaaac aaaaagccca gctgcacgcc
      121 agtgtgcggc ctgaagattt ggtgcaggcc tttgtggatg tgtgtttgca atacgtgcgt
      181 gattttgacc gtgaagcggt gttggtcacg cgcttggtga cgcgtttgat gttggatgag
      241 tacaaggtgt ttcgtgagca attggcacag gaatacaccg acatcattga acacattttg
      301 catttgtttg aacaggcttt gccgcatttg gacaaggaaa ccgtgcgttg gcgcatgcac
      361 attgcactga gtactttgtt caacagtttt gctggcaatg atgtgttaaa agccttggcc
      421 cgtcgtgaat gggtcaatgc taaaaatccc gagcaagtgg cgcgctatgt ggtgccattt
      481 gtggttgcag gtttagtcgc gccctaatca aggcgcagtc aattaaggag aagaagtaat
      541 gttaagtttg ggcttaatgc tactgctcgg tggaatcgct gcttattttc gtgccaaagg
      601 ctggatgatt gctttggcgg cgtttgtggg tgcgctcatc ggtggttcag tgtggtgggc
      661 tttgttgcct ttggtggcag gagtggtgtg ttgtttgccg tttgtaaaat ctttgtacat
      721 gcctaaagta tttgccatgt tcaaaaaagt gacgccgccg atgtcggaaa ccgagcaaga
      781 tgcgattaac gcaggcacgg tgtggtggga tggcgagttg ttttcaggca agcccgacta
      841 ccaaaaattg ttgaactacc cacacccaca attgactgca gaagagcagg cattcattga
      901 tggccctgtg gaagaattgt gcaacatgct caatgattgg caaatcaatc acgaattgaa
      961 agacttgcca caagaggcgt gggatttcat caagcagcag ggcttcttgg gcatgattat
     1021 caagaaacaa tatggcggtt tggaattctc caattatgcc catgccaaag tggtgaccaa
     1081 aatcgctacc cgttgtggca gtgccgctgt gacggtaatg gtgccgaatt ctttgggccc
     1141 tggtgagcta ttgcaacact atggtaccga agcgcaaaaa gacttttatt tgccacgttt
     1201 ggccaaaggt gctgacattc catgttttgc cttaaccagt ccttcagctg gctctgatgc
     1261 aggcggcatt cctgactttg gggtggtgac gcgtggttct tacaccgatc ctcacacagg
     1321 cgagcgccat gaagacgtat tgggtgtgcg agtgtcatgg gaaaaacgct ggattacctt
     1381 ggcccctgtg gccacagtat tgggcttggc cttcaagatg tttgaccccg atggtttgtt
     1441 gggtgacaaa aaagaaattg gcattacctg tgccttggtg cctacccatc atgagggtgt
     1501 gcaaattggt cgccgtcact ggccatcagg cagtgcgttc atgaatggcc caacttgggg
     1561 tgcggatgtg ttcattcctt tggattggat catcggtggt gtggactacg caggtcgtgg
     1621 ttggcaaatg ttggtggaat gcttgtctgt aggccgttgt atttctttgc ctgccaactc
     1681 tgtggcagca ggcatggtca gttcttacac caccagtttg tatgcccgca ttcgtgatca
     1741 atttggtttg ccaattggca agttcgaagg tgtggacgag gctttggcgc gcatcattgc
     1801 caatacttat caaatggaag cctcacaaga tttggctttg acaggtttgg atgtaggtga
     1861 aaaaccaagt gtgatttcgg cgatgttgaa atatcacaat accgaacgca tgcgcaaaac
     1921 cttgaacgat gctatggaca tacatggcgg tcgtactgtg gtacaaggcc cgcgtaatta
     1981 tttggtgcca gcttaccaag cgattccaat tgccattacg gtggaaggcg cgaatatctt
     2041 gacccgcagc ttgatgatct tcggtcaagg cgcgattcgc agccatcctt atgtgttgcg
     2101 tgaaatgatg gcggctacca ataacgatgc ggcttcattc aatcaagtct ttggtcagca
     2161 cgtgcatttt gtgttcacga atgtggcgcg cagtttctgg ttgggcttga ccaacggctg
     2221 tttgagcaaa gccccacaac gtggcgtgac cgcaccgtat tacaaacaaa tgaaccgtgt
     2281 ggcggccaac ttcgccttgt taaccgatat gtgcatgttc agcttgggtg gttcattgaa
     2341 attccgcgag aaattgtcgg ctcgtttggg cgacattgta gcgaatttgt acatctgttc
     2401 tgctgtgttg aaacgctttg agcgtgacgg ttcacaaaaa gccgatgtgg ccatcatgca
     2461 gtatgctgcg caacaggctt tgtttgatgc ccaagtggcc atggatggtg tgttgaaaaa
     2521 cctgcctaat cgtccgcttg cgtggttgtt gcgtggcttg accatgccta tggggcacgc
     2581 catcaaagca cctagcgata cattgggtac gcaagtggcc aatgaagcca tgaagggtga
     2641 agaagcttta gagcgtttga cacacttcat gttcaagtca agtgatgaga gcgatgctgt
     2701 gggtgtgttg gctccagccc acaaagcggt gattgcggct gccgatgctg agaaaaaatt
     2761 gcgcaaactt gaacgtgatg atcagctgga tgctccttct ccaaacttgc gtctggaaca
     2821 ggctttgcaa ttgtcattaa tcagtcaagc agagtttgat ttaatcagca aagcccgcaa
     2881 tttgaaacgt caagtaatta tggttgatga tttcgatatg cagcttgagc aacatgatga
     2941 taaattgtta gagcgtcaca tggtaatacc cccattttta attgaattaa tgggtagaaa
     3001 atctaaagcc ttgtt
//
LOCUS       DAOMVK010000277         2993 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_9722_length_2993_cov_2.961198, whole genome shotgun sequence.
ACCESSION   DAOMVK010000277 DAOMVK010000000
VERSION     DAOMVK010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9722_length_2993_cov_2.961198"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="PK856_08865"
     CDS             complement(<1..973)
                     /locus_tag="PK856_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HRL02517.1"
                     /translation="MSSNKLVVRKVAVLGAGVMGAQIAAHLANAKVPTVLFDLPAKEG
                     PKNAIVEKALVALKKQKPAPLASKTAIQYIQAANYDDDLALLSECDLVIEAVAERLDI
                     KENLYSKVAPHLGAKTIFATNTSGLSIETLAGVFSKELKDRFCGVHFFNPPRYMHLVE
                     LIACKETDASVLDNLERFLVSTLGKGVVRAKDTPNFVANRIGVFSMLATMANAEKYDL
                     RFDVVDDLTGARLGRPKSATFRTADVVGLDTFAHVAKTMQDNLQADPWHAHFGLPTWL
                     TGLIEKGLLGSKTKAGIFKKEGKRIFVLDPKAGDYVASTEKADKAVTE"
     gene            complement(1041..2816)
                     /locus_tag="PK856_08870"
     CDS             complement(1041..2816)
                     /locus_tag="PK856_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HRL02518.1"
                     /translation="MNKNVFTSIKLSTILITAGIAAQAHASGYHFGTQSVSSQATANS
                     SAAEAADPSTIFYNPAGLTKLEGTQATINLNLVIPSIDSYDQKGYYPTDANGSPAAAG
                     KGEAIKGSSKDSIDPGLTFAPHAYASHQVNDRVSVGLGVFVPFGSGTEYERDSVLRYN
                     VNELGLQTIAIQPTVAFKLNDQHSFAVGLVAQNTSAELRQYANFGPAVASGLSAKSGE
                     AAAGLTKLNAGIAQLNGGIAQYNAGIAQADAGIAQLQAALQANPNNPQLQAQLAATQA
                     ARDKAVTDRTGLQTQLAGYTAQRDGVAQQKQVVDAQLAQMGKSGNGAADGHAKVKGDD
                     WGFGYNLAWMWDINDAVRVGVNYRSKVTHNLKGHAEWYLEDGAFNNPYLGSAIKQTIR
                     DRGYVSKETASVKIVTPESLSLHGMWKVDPKWNLFADATWTRHSRFNQAILQYENMKT
                     VLPAADGNKSNKTVLNPNWKDTWKFGVGASYQITEPLQLRFGLAYDQSPVRSSEYRMS
                     TLPDNDRIWFSLGGKYDFNKHHSVNVAYSYIYIKDAEAQVNGYGTDKVAGGIGSTASV
                     SSKTNGSAKFDSNAHILGVQYTYRF"
BASE COUNT          776 a          735 c          687 g          795 t
ORIGIN      
        1 actcggtaac ggctttgtcg gctttttcag ttgaagccac gtaatcgccg gctttagggt
       61 cgagcacaaa aatgcgtttg ccttcttttt tgaagatgcc cgctttggtt tttgagccca
      121 gcaagccttt ttcaatcaag cccgtcaacc aagttggcaa accaaaatgg gcatgccaag
      181 ggtcggcctg taaattgtct tgcatggttt tcgccacgtg tgcaaacgtg tccaagccca
      241 ccacatcggc agtgcggaac gttgcagatt tcgggcgacc caaacgtgcg cccgtcaaat
      301 catcgaccac atcaaaacgc aaatcgtatt tctcagcatt cgccatggtc gcaagcatgg
      361 agaacacgcc tatgcggtta gcgacaaaat taggcgtgtc tttggcgcga accacgcctt
      421 tgcccaaagt cgataccaag aaacgctcta aattatccaa aaccgaagca tcggtttctt
      481 tgcaggcaat caattccacc aaatgcatgt aacgtggtgg attgaagaaa tgcacgccgc
      541 agaagcggtc ttttaattct ttggaaaaca caccagccaa agtctcaatg gacaggcctg
      601 acgtgttggt cgcaaaaatg gttttagcgc ccaaatgcgg ggccactttg ctgtacaggt
      661 tttctttgat gtccaaacgc tcagccacag cctcaatcac caaatcacat tcagacaaca
      721 aagccaaatc atcatcgtaa ttggcggcct gtatgtattg tatggccgtt ttactggcca
      781 acggtgctgg cttttgcttt ttcaatgcga ccaaggcttt ttccacgatg gcgtttttag
      841 gaccttcttt ggcaggcaaa tcaaataaca cagtcggcac tttggcattg gccaagtggg
      901 cagcaatttg agcacccatc acgcctgcgc ccaaaacggc cactttgcgt accacgagct
      961 tgtttgaaga cataagaaaa cctctttact gacagggttg taattcatgc gagtgggttg
     1021 ccccacccgc gttgacttga ttagaaacgg taagtgtatt gcacacccaa aatgtgggca
     1081 ttgctgtcaa atttggcaga accattggtt ttgctgctga cgctggctgt tgaaccaata
     1141 cccccagcca ctttatcagt gccgtaacca ttcacttgag cctcagcatc tttgatgtag
     1201 atatagctgt aggccacgtt tacagaatgg tgtttgttga agtcatactt accacccaat
     1261 gagaaccaga tgcggtcgtt atctggtaag gtagacatgc ggtattcaga gctgcgcact
     1321 ggcgattggt cataagccaa accaaagcgc aattgcaaag gctcggtgat ttggtaagac
     1381 gcacccacac caaatttcca agtgtctttc caatttggat tcaacacggt tttgttagac
     1441 ttgttgccat ccgctgctgg caatacggtt ttcatattct cgtattgcaa gatggcctgg
     1501 ttaaagcgcg agtggcgtgt ccatgtagca tcggcaaaca gattccattt tggatccact
     1561 ttccacatgc catgcaaaga caaagattca ggtgtcacaa ttttcacact ggcggtttct
     1621 ttggaaacat agccacggtc acgaatggtt tgcttgatgg cactgcccaa ataaggattg
     1681 ttaaacgcgc catcctctaa ataccattcg gcatggcctt tcaaattatg ggtcactttg
     1741 gagcggtagt tcacacccac acgcacggca tcattgatgt cccacatcca agccaagtta
     1801 taaccaaaac cccagtcatc gccttttacc ttggcatgac catcagcagc gccattgcca
     1861 ctcttaccca tttgagccaa ctgtgcatct acgacttgtt tttgttgggc cacaccatca
     1921 cgttgtgcgg tgtatcctgc caactgagtt tgtaaaccgg tacgatcagt gactgctttg
     1981 tcacgagcgg cttgggtagc tgctaactga gcttgcaatt gagggttatt gggattggct
     2041 tgtaatgcgg cctgtaactg tgcaatacca gcatctgctt gagcaatacc tgcattgtac
     2101 tgagcgatgc caccattcaa ttgagcgatg cccgcattca atttggtcaa accagcggca
     2161 gcctcacctg acttggctga caggcccgaa gccacagcag ggccgaaatt cgcatattga
     2221 cgcaattctg cagacgtatt ttgcgccacc aaacccacgg caaaagagtg ttggtcattc
     2281 agtttgaacg cgacagtcgg ttggatggcg atggtttgta agcccaattc atttacgttg
     2341 tagcgcaaaa ccgaatcacg ctcatattca gtgcctgagc caaatggcac aaacacaccc
     2401 aaacccactg acactcggtc attgacttga tgagacgcat aagcatgagg ggcaaaagtt
     2461 aaccctgggt cgatgctgtc tttgctgctt cccttaatgg cttcaccctt gcctgcagca
     2521 gcgggactgc catttgcatc cgtagggtaa tagccttttt ggtcataaga atcaatgcta
     2581 ggtatcacca aattcaaatt gatggtggct tgtgtgcctt ccaatttggt caaaccagct
     2641 gggttataga aaatggtaga aggatcggca gcttctgcag cagaagaatt tgctgttgct
     2701 tgtgaactga cagattgggt accaaagtga taacccgatg catgtgcttg tgcagcaata
     2761 cccgcagtaa tcaaaatggt tgaaagttta attgaagtga atacgttttt attcatgctt
     2821 tatttctcct tggttcatgc caatgcaata taaaaaaggc agccattgta tgtttttttt
     2881 gctacccagt cagacaaatc ccccactgcc tagcatgcat agtgcaggca gcggggatta
     2941 ggcatcaaaa tatgtggtaa tcctccctaa aaataattga gtttaaaagt aga
//
LOCUS       DAOMVK010000278         2990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_9738_length_2990_cov_3.994889, whole genome shotgun sequence.
ACCESSION   DAOMVK010000278 DAOMVK010000000
VERSION     DAOMVK010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9738_length_2990_cov_3.994889"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..550)
                     /locus_tag="PK856_08875"
     CDS             complement(80..550)
                     /locus_tag="PK856_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02519.1"
                     /translation="MLKKLSLGLMVLASTSAVMAAGITAQDAGVYDVVSIKDNKPVGL
                     SGIQMRIYQKGGDWFMDGKDDNIKSGPAKGKWFPVCNDGNKCEFKTSSKSDLKKIFPD
                     LAVIQKTDDIGCIQNEVQAICRLDSKVQKGYTGYMTVVLQAKPHVYMTMQRRPS"
     gene            complement(612..1151)
                     /gene="hslV"
                     /locus_tag="PK856_08880"
     CDS             complement(612..1151)
                     /gene="hslV"
                     /locus_tag="PK856_08880"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HRL02520.1"
                     /translation="MQQFDGTTIVSVRRGEHVAIGGDGQVTLGNTIIKATARKVRKLY
                     NNQVLAGFAGGTADAFTLIELFESKLQKHQGKLMVAAIELAKEWRTDRALRRLEAMLI
                     VADKQYTLIITGNGDVLEPEDSIAAIGSGGAFAQSAARALMENTDLAPEVVVKKSLEI
                     AGGICIYTNQNHTIETLEA"
     gene            complement(1260..1670)
                     /locus_tag="PK856_08885"
     CDS             complement(1260..1670)
                     /locus_tag="PK856_08885"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRL02521.1"
                     /translation="MNFSPFVMFDGDCEQAFGYYHSVFGGELEVLRYSDAPLLDKPEA
                     QMDNKIMYAQLQINDQKLMGCDANDTMYQPQQGVLVAWQGETEAEVERVFAALAQGGM
                     VRQALNYAFWTPKYGLVEDKFGMVWMISCRKSEE"
     gene            complement(1689..>2990)
                     /gene="mutS"
                     /locus_tag="PK856_08890"
     CDS             complement(1689..>2990)
                     /gene="mutS"
                     /locus_tag="PK856_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRL02522.1"
                     /translation="HIQTHGDEFLLDLEARERERTGLSTLKVEFNRVHGFYIELSKAQ
                     AEQAPADYQRRQTLKNVERYITPELKVFEDKVMSAQDQALALEKQLFDGLLRELQAAL
                     PVLQRIAKAAASLDVLAALAKIAAQEQWVQPELVDFPCVEIRQGKHPVVAAQVAQFIA
                     NDARLDGKHKLMLLTGPNMGGKSTFMRQTALIVLLAHVGSLVPAQSARIGMVDQIFTR
                     IGASDDLAGNRSTFMVEMSESAYILHHATERSLVLMDEVGRGTSTFDGLALAQAIAEY
                     LLQKNKSLTLFATHYFELTKLPERFQDAFNMHLNALEQSQDIVFLHQVEAGPASKSYG
                     VAVAKLAGLPTKALHSAQQYLHQLEQKQREQQAQLGLFQDWEAEAATEMPMQAQVTTP
                     DTDSKYAACIEALQRMQPDELTPRAALDVLYKLQALLPDGD"
BASE COUNT          710 a          806 c          670 g          804 t
ORIGIN      
        1 ttcttgaggt gtcatcactg ctgctgacat agtgtttcgc tttcctgatg gccgcctgct
       61 cacaagcggc cggtaatgat tatgatgggc gacgttgcat ggtcatgtac acatgcggtt
      121 tggcttgcaa caccaccgtc atgtagccgg tatagccttt ttgtactttg ctgtctaaac
      181 ggcaaatcgc ctgtacttcg ttttgaatac aaccaatatc atcggttttt tgaatgacgg
      241 ccaaatcagg gaatattttt ttcaaatcgc ttttagatga ggtcttgaat tcacatttgt
      301 taccatcatt gcacactgga aaccatttgc ctttggctgg gcctgatttg atattgtcgt
      361 ctttgccatc cataaaccaa tcgccacctt tttggtaaat gcgcatttgt atgcccgata
      421 agcccacggg tttgttgtct ttgatggaga ccacatcgta aacacctgcg tcttgtgcgg
      481 taatgcctgc tgccatcacg gcgcttgtgc tggccaaaac catcaaaccc aaactcaatt
      541 ttttcaacat actcatttcc ttttcagtcg gtcatgcggc tgtacatcat tcttcggtgt
      601 acaaactgga tttatgcttc taaggtttct atcgtgtgat tttggttggt gtaaatacaa
      661 atgccacctg caatttccaa agatttcttc accaccactt caggagccaa atcggtgttt
      721 tccatcaagg cacgggcggc agattgcgca aatgcgccac cagaaccaat ggcagcaatg
      781 ctgtcttctg gctccaatac atcgccattg ccggtaataa ttaaggtgta ttgcttgtcg
      841 gccacgatca gcatcgcttc caaacggcgc aaagcgcgat cggtacgcca ctcttttgcc
      901 aattcaatcg ccgccaccat caatttgcct tggtgttttt gcaatttgct ttcaaacagc
      961 tcaattaagg tgaacgcgtc ggcggtaccg ccagcaaatc ctgccaaaac ctgattgttg
     1021 tacagcttgc gcactttgcg tgcggtggct ttgatgatgg tgttacccaa agtgacttgg
     1081 ccatcgccgc caatggcgac gtgttcaccg cgtcgtaccg acacaatggt tgtgccgtcg
     1141 aattgttgca tactgactcc ttaactgatg cggcctgtaa cgccgccttt gccacaaagg
     1201 tgagggcaaa acagcctgac atcaagcagg ctgcataaaa taatctgttt acaggccgct
     1261 tattcttcgc ttttgcgaca cgaaatcatc cacaccatgc caaatttgtc ttcaaccaag
     1321 ccatatttgg gcgtccaaaa cgcgtaattc aaggcttgcc tgaccatgcc accttgtgcc
     1381 aaagccgcga acacccgctc cacttctgct tcggtttcac cttgccatgc caccaacacg
     1441 ccttgttgtg gctggtacat ggtgtcgttg gcatcgcaac ccatcaactt ttggtcattg
     1501 atttgcaatt gggcatacat gattttgttg tccatttgcg cctcaggctt gtctaataaa
     1561 ggcgcatcgc tgtaacgcaa cacttctaac tcaccaccaa acacactgtg gtaataccca
     1621 aaagcctgct cgcaatcacc atcaaacatc acaaatggac tgaaattcat gttaactctc
     1681 ctgtgggttc aatcaccgtc gggcagcaaa gcctgtaatt tgtacaagac atccaaagcc
     1741 gctcgcgggg tcaattcatc gggctgcatg cgctgtaaag cctcaataca agccgcatat
     1801 ttgctgtcgg tgtctggtgt tgtcacctgt gcctgcatgg gcatctcagt cgcagcttct
     1861 gcttcccaat cttgaaacaa gcccaattgt gcctgttgct cgcgttgctt ttgttctaat
     1921 tggtgtaaat attgttgcgc ggaatgtaaa gccttagtgg gcaaacccgc caatttcgcc
     1981 accgccacac cataactctt gctcgctggc cctgcttcca cttggtgcaa aaacacaatg
     2041 tcttgacttt gctccaaggc attcaaatgc atgttgaacg catcttgaaa acgctcaggc
     2101 aatttggtca gctcgaaata atgggtggca aacagggtta aagatttgtt tttttgcagc
     2161 aagtattcgg cgatggcttg agccaaggcc aagccatcaa acgtggacgt accgcgcccc
     2221 acttcatcca tcaacaccag tgaacgctcg gtggcgtggt gcaaaatgta agccgactct
     2281 gacatttcca ccataaaggt agagcggttg cccgccaaat catccgatgc accgatgcgg
     2341 gtgaaaattt ggtcgaccat gccgatgcgt gccgactgcg caggcaccaa tgagcccaca
     2401 tgcgccaaca acacaatcaa ggcggtttgg cgcataaacg tggatttacc gcccatatta
     2461 gggccagtga gcaacatcag tttgtgtttg ccatccaaac gcgcgtcatt ggcaatgaat
     2521 tgcgccactt gcgccgccac cacagggtgt ttgccttggc gtatctccac acaaggaaaa
     2581 tccaccaact caggctgtac ccattgttct tgcgcggcga tttttgccaa ggccgccaat
     2641 acatccaagc tggcagcggc tttggcgatg cgttgcaaca caggcaatgc ggcctgtaac
     2701 tcacgcaaca agccatcaaa caattgcttt tccaaagcca aggcttggtc ttgcgcactc
     2761 atcaccttgt cttcaaacac cttcaactcg ggcgtgatgt agcgctcgac attttttaag
     2821 gtttggcggc gttggtaatc tgcaggcgct tgctcagctt gggctttaga caactcaatg
     2881 taaaaaccat gcacgcggtt aaattccacc ttgagcgtgg acaagcctgt gcgctcgcgc
     2941 tcgcgcgctt ccaaatccaa taaaaactca tcgccatggg tttgaatgtg
//
LOCUS       DAOMVK010000279         2978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_9791_length_2978_cov_1.835443, whole genome shotgun sequence.
ACCESSION   DAOMVK010000279 DAOMVK010000000
VERSION     DAOMVK010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9791_length_2978_cov_1.835443"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1203)
                     /locus_tag="PK856_08895"
     CDS             complement(<1..>1203)
                     /locus_tag="PK856_08895"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRL02523.1"
                     /translation="TVVTQADLQEKSYTNLAEALSDVPGVDIRNGVGKTGGLNIQMRG
                     MPSEYTLVLIDGRRQNTSGSIAPNGFGEMANGFMPPLSQIQRIEVIRGPMSTLYGSDA
                     MGGVVNIITKPVSSGEWGGNITADTTLHQYEGGNTRQIGFNASGPIVKDTLGIQVRGR
                     YFDRGESERMIENGTSRDPRPGESKNYEFGTKLTYNLNENHELWADVAHSRQRYSNED
                     SRLGTLDFADLPSSRPGLPSSQRARGYKDFMAMEKDQFAIGHVSEFELGTWQSYVSQV
                     KAETTGRTMPYGYTVNDNSYRFNGENRTLKNTDTTVNTKFNTELGAHNLTVGAEYQDN
                     ETIDRAAGDGVKFTQDSWAVFLEDQWKILPNLSMTMGGRYEDHSSFGGHFTPRAYLVW
                     NTTDKITLK"
     assembly_gap    1204..1213
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1214..1403)
                     /locus_tag="PK856_08900"
     CDS             complement(<1214..1403)
                     /locus_tag="PK856_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02524.1"
                     /translation="MRTRFFHSTLSVAILAAFAMPALADTTTQSAEADTELEEIVVTA
                     SGSGSNLKNAPASVSVIHE"
     gene            complement(1709..2282)
                     /locus_tag="PK856_08905"
                     /pseudo
     CDS             complement(1709..2282)
                     /locus_tag="PK856_08905"
                     /inference="COORDINATES: protein motif:HMM:NF015786.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(2345..>2978)
                     /locus_tag="PK856_08910"
     CDS             complement(2345..>2978)
                     /locus_tag="PK856_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HRL02525.1"
                     /translation="KGAFGACLMNEVDLVADGLKAMQDVVDIDVTVKHRIGVDKQEEY
                     AVVRDFVGTIAEKAACKTFIVHARNAWLQGLSPKRDFPELEIILNGGVTDNAQVAEHL
                     QYVDGVMVGRSAYHQPLSMMAQWDALFYGDAHEVMTFEALLPLLQSYAESHLQADKGT
                     QLRHMARHYLGLSHGMKGARQWRRMLSDANLLADNRADLIAEAGAALNLR"
BASE COUNT          760 a          756 c          683 g          769 t
ORIGIN      
        1 ttttaaggtg attttatcgg tggtattcca caccaaataa gcgcgagggg tgaagtggcc
       61 accaaaactg ctgtggtctt catagcgacc gcccatggtc atgctcaaat taggcaagat
      121 tttccactgg tcttccaaaa acacagccca tgaatcttgg gtaaatttca ccccatcgcc
      181 tgcggcgcgg tctatggttt cattgtcttg gtattcggca ccaacggtaa gattgtgtgc
      241 gcccaattcc gtattgaatt tggtgttcac cgtggtatcg gtgtttttca aggtgcggtt
      301 ttcaccgttg aaacggtatg aattgtcgtt gactgtgtaa ccataaggca tggtgcgacc
      361 tgtggtttcg gctttgactt gtgacacata gctttgccat gtacccaatt caaattccga
      421 cacatggccg atggcgaact ggtctttttc catcgccata aagtctttgt aaccccttgc
      481 gcgctgcgaa gacggcaaac caggccgtga acttggcaaa tcggcaaaat caagcgtgcc
      541 caaacggctg tcttcattgc tgtaacgctg acgagaatgc gccacatccg cccacaattc
      601 gtgattttca ttcaagttgt acgtgagttt ggtgccaaat tcgtaatttt tagattcacc
      661 tggacgaggg tcacggctgg tgccattttc aatcatgcgc tcagactcac cacggtcaaa
      721 atagcgacca cgcacctgta tgcccaacgt gtctttgaca atagggccgc tggcattgaa
      781 gccaatttga cgggtattgc cgccttcata ttggtgcaaa gtggtatcgg cagtgatgtt
      841 gccaccccac tcaccagaag aaacaggctt ggtgatgatg ttcaccacac cacccatggc
      901 atctgaacca tacaaggtgg acatggggcc acggatgact tcaatgcgct gaatttgcga
      961 caaaggtggc ataaagccat tggccatttc accaaaacca ttcggtgcaa tcgaaccaga
     1021 agtattttga cggcggccat caatcaaaac caatgtgtat tcagaaggca tgccacgcat
     1081 ttgaatgttc aggccgcctg ttttgcccac gccattgcga atatcaaccc cagggacatc
     1141 tgacaatgcc tcggccaaat tggtatagct tttttcctgc aaatcagcct gtgtcaccac
     1201 ggtnnnnnnn nnncctcatg tatcacagac acactggcag gcgcattttt caaattgctg
     1261 cctgagccgc tggcagtcac cacaatttct tctagctcgg tatcggcttc ggctgattgt
     1321 gtggtggtgt cggccaaagc gggcatcgca aaggccgcca atatggcaac agacaaggtt
     1381 gaatggaaaa agcgcgttcg catggctacc taccccttaa ggttaacttt aaagaaacaa
     1441 atacagtcca acactgtaaa ttcgcagaga ttataattga tactcactct caatatcaat
     1501 tacaaattga tttaaatggc aataactgct gaatacttct taaaaataag cttacttgcc
     1561 taaaaatttc aataaaatta gaaattgttt gaatccgatt gatcgacaca cttacagata
     1621 aattcacaat actgttaacc taaaataaaa aatcggcctg taaatgttta caggccgatg
     1681 gcaaacttta tggattttga agcgtgattt aagcgcgttt gcgtgcccac atggtggcga
     1741 aattcaagca aatgtgattg ccattggcat cggtgcggtg caaagaacct tcgtcttcgt
     1801 tgtatttcac gatgtcccaa tctgcataca attcacgcaa ttgattgggt ttgaataaaa
     1861 attgcaatac cgatggcaca ttcgcgccct cagtttccat cgcactgacg atgagattcc
     1921 agccgcctac tgccgtttgc gcttgcatat tgtgaatgat gtcggctatg cgttcgggtt
     1981 ttaaaaactg cgtaagtgga ttgcaccacg gcacgattga tgtcgtaaac atcggtttcg
     2041 atgttgtcca tgttttcggc ggtgatgatg tgattcaagg tggcaatgct ggcttcgtcg
     2101 gcatcacacg catgcacatc aaagccatgc ttggccaaat acaaggcatt gcggccgcgg
     2161 ccacagccca aatccaagac atgtccgtgg ttcaaagggc cagcctccac ccacgcttgc
     2221 acttcagaat gggtggtgga taagccgtag tgttgtttga aatagtcggg actgatgctc
     2281 atggtgcgac aacctttgcg cgaaaaagaa acatcatgcc acagcggttt acaggccgct
     2341 tgctttatcg caagtttaaa gccgcgcctg cttcggcaat caaatcggct cggttgtctg
     2401 ccaacaaatt ggcatcagac aacatgcgac gccattgtct tgcgcctttc atgccatggc
     2461 tcaagcccaa ataatgccgt gccatgtggc gcaattgcgt gcctttatcg gcctgtaaat
     2521 ggctttcggc atagctttgc aacaacggca acaaggcttc aaacgtcatg acctcatggg
     2581 catcgccgta aaacagtgca tcccattgcg ccatcatgga aagaggctgg tggtaggcgc
     2641 tgcgccccac catcacccca tccacatatt gcaaatgctc agccacttgc gcgttgtcgg
     2701 taacgccgcc attcaaaatg atttccaact cggggaaatc gcgcttcggt gacaaacctt
     2761 gcagccacgc attgcgtgca tgcacgataa aggttttaca ggccgctttt tcagcgatgg
     2821 tgccaacaaa atcacgcacc acggcgtatt cttcttgctt gtctacaccg atgcggtgct
     2881 tcaccgtcac atcaatgtcc accacgtctt gcatggcttt caagccatcg gcgaccaaat
     2941 ccacctcatt catcaagcaa gcgccaaacg cgcctttt
//
LOCUS       DAOMVK010000280         2975 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_9803_length_2975_cov_3.645205, whole genome shotgun sequence.
ACCESSION   DAOMVK010000280 DAOMVK010000000
VERSION     DAOMVK010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9803_length_2975_cov_3.645205"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..595)
                     /locus_tag="PK856_08915"
     CDS             complement(161..595)
                     /locus_tag="PK856_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HRL02526.1"
                     /translation="MNTVKKNVLVTHTAEQMFALVDDIEHYADFLPWCNQSVVHKREP
                     NMVEATVGIDYMRLKQSFSTRNTNIDGREIRMQLLEGPFKSLEGIWKFTPFGDIGCRI
                     DFQLQYEFANGLISHIIAPVFGRISGTLVDSFIKEADKRHGK"
     gene            841..1296
                     /gene="smpB"
                     /locus_tag="PK856_08920"
     CDS             841..1296
                     /gene="smpB"
                     /locus_tag="PK856_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003740866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRL02527.1"
                     /translation="MSIISNKKAFHDYFIEEQLEAGLVLEGWEVKAIRAGRVQIKESY
                     VYFRDGAFYLIGCHITPLPEASTHVNPDVTRSRKLLMKQGEIEKWFGKVERAGLTVVP
                     LNLHYSRGRIKADIGLAKGKKLHDKRESEKDKDWQREKQRIMKHSNNDS"
     gene            1313..1660
                     /locus_tag="PK856_08925"
     CDS             1313..1660
                     /locus_tag="PK856_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02528.1"
                     /translation="MRSRSLFIPLFLIVMGSLWFIKSIDLMPETSSIIAIGLAVLGLV
                     VLITDGINKQSIVAGPFLMYLGAAVYLYYEEWFRIPHILSVGMVVLGSLLLLSRSGMV
                     PDKYARHLFPPQD"
     gene            1675..>2975
                     /gene="guaA"
                     /locus_tag="PK856_08930"
     CDS             1675..>2975
                     /gene="guaA"
                     /locus_tag="PK856_08930"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRL02529.1"
                     /translation="MDKILILDFGSQVAQLIARRVREAHVYCELHPFDMSLEDIKAFQ
                     PNGIILSGGPNSVYESDYQADTGLLELGIPVLGICYGMQWMAHVMGGEVASGNQREFG
                     YAEVLVSPSRLLESLTDDSNMLEVWMSHGDKVSKLPQGFEVVGKTPSCPIAIMQNTAK
                     NFYGVQFHPEVTHTIKGRELLNHFVLDICGAQPGWTMPNYIDEAITKIREQVGTDEVI
                     LGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGTMVMDMFARNLGVKVIHVQ
                     AEADFMGKLAGETDPEKKRKIIGAEFVEVFQRESGKLTNAKWLAQGTIYPDVIESAGG
                     KKNKAHAIKSHHNVGGLPEDMHLQLLEPLRDLFKDEVRELGVALGLPREMVYRHPFPG
                     PGLGVRILGEVKAEYADLLRRADDIFIQELRNFKDEQGVSW"
BASE COUNT          759 a          686 c          742 g          788 t
ORIGIN      
        1 tgccttacca aatatgccca tcgggtagtc ttgtgtgtgt ggaaattgcg tgtacaggcc
       61 gcttaattgc accgcagcca aggctgttgt gcctgcgtct acttccaagc ccaccaagta
      121 ttgttgttct ttggtggcgt acaccacctc tacagtgagt ttatttgcca tggcgtttgt
      181 cggcttcttt gatgaatgaa tcgaccaaag tgcctgaaat acggccaaaa acaggggcga
      241 tgatatgcga tatcaagcca ttggcaaatt catactgcaa ttgaaaatca atgcgacagc
      301 ctatgtcacc aaacggggta aatttccaaa tgccttccag tgatttgaat ggaccttcga
      361 gcaattgcat gcggatttcg cgcccatcga tattggtgtt gcgggtagaa aagctttgtt
      421 tcaagcgcat gtaatcaatg cccacggtgg cttctaccat attcggctcg cgtttgtgca
      481 ccaccgattg gttgcaccaa ggcagaaaat ccgcgtaatg ttcaatatcg tcgaccaaag
      541 caaacatttg ctctgctgtg tgtgtcacca agacattttt tttaacggtg ttcatgtact
      601 cgttttctag tcgcgtttca ttcaaaagtt ttgcatgcga acactcgcat ccatgacgtt
      661 catgcattta ttgcgtgcca tgttaatggc aaaaccgctg ttgcttctgc cccaattagg
      721 gtaagcaaat cacttgcgcg gcaacaaacc gctattatgc cattaaatac cttggggcga
      781 aaatccacgc aagcgtcgct ttctgttaaa atagcggcct gtaattttag gggcgctgtc
      841 atgagcatca tcagcaataa aaaagccttc catgactact ttattgaaga gcagctggaa
      901 gcgggtttgg tattagaagg atgggaagtc aaagccatcc gcgctggccg tgtgcaaatc
      961 aaagaaagct acgtgtattt tcgcgatggc gcgttttatc tgattggttg ccacatcacc
     1021 cctttgcctg aagcctcgac gcatgtcaat cctgatgtga cgcgttcgcg caaattgctg
     1081 atgaagcaag gcgaaatcga gaaatggttt ggcaaagtag aacgtgcggg cttgactgtg
     1141 gtgccattga atctgcatta cagccgtggc cgcatcaagg ccgacattgg cttggccaaa
     1201 ggtaaaaaac tacacgacaa acgtgaaagt gagaaagata aagactggca gcgcgaaaaa
     1261 caacgcatta tgaagcattc taacaatgat tcttaagaag gcgttttaaa ccatgcgcag
     1321 tcgttctttg tttatccctt tatttttaat tgtgatgggc agtttgtggt ttatcaaatc
     1381 catagacttg atgcccgaaa cctcttccat catcgccata ggattggcgg tgttgggtct
     1441 ggtggtgtta atcaccgacg gtattaacaa gcaatccatc gtggccggcc cgtttttgat
     1501 gtatttgggc gcagcggtgt atttgtatta cgaagagtgg tttcgcattc cccatatttt
     1561 atcggtgggc atggtggttt tggggtcttt gttgttgctc tcgcgcagcg gcatggttcc
     1621 agacaaatat gcccgccatc tgttcccccc acaagattga tttgagaatt tctgatggac
     1681 aaaatcctga ttttagattt cggctcacaa gtggcgcagc tgattgcgcg tcgtgtgcgt
     1741 gaagcccatg tttattgtga attacacccg tttgacatgt ctttagaaga catcaaagcg
     1801 tttcagccca acggcattat tttgtctggc ggccctaact cagtgtatga atccgattac
     1861 caagccgaca caggtttgtt ggaattaggc attcctgtat tgggcatttg ctacggcatg
     1921 caatggatgg cgcatgtgat gggcggcgaa gtggcttctg gtaaccaacg agaatttggt
     1981 tatgccgaag tattggtaag ccctagccgt ttgctcgaaa gcttaaccga cgacagcaat
     2041 atgttggaag tgtggatgag ccacggtgat aaagtgtcaa aattgccaca aggctttgaa
     2101 gtggttggca aaaccccaag ctgcccaatt gccatcatgc aaaacaccgc aaaaaacttc
     2161 tacggcgtgc aatttcaccc agaagtgacc cacaccatca aaggccgtga gttgttaaac
     2221 cactttgtct tagacatttg tggcgcgcaa ccaggctgga ccatgcccaa ctacatcgac
     2281 gaagccatca ccaaaattcg cgaacaagtc ggcactgacg aggtgatttt aggcttgtct
     2341 ggcggcgtgg attcttctgt ggccgcagcc ttgattcacc gcgccatcgg cgaccaattg
     2401 acttgcgtgt ttgtagacca cggtttgctc cgcttgaacg aaggcacaat ggtgatggac
     2461 atgttcgcgc gcaatttggg cgtgaaagtc atccatgttc aggccgaagc cgatttcatg
     2521 ggcaaattag ccggtgaaac cgaccctgag aaaaaacgca aaatcattgg cgctgaattt
     2581 gtcgaagtgt tccaacgtga atctggcaaa ttaaccaatg ccaaatggtt agctcaaggc
     2641 accatctatc ctgacgtgat cgaatcggca ggcggcaaga aaaacaaagc ccatgcgatt
     2701 aaatcgcacc acaacgtggg cggcttgcca gaagacatgc acttgcaatt gttagaacct
     2761 ttgcgtgact tgttcaaaga cgaagtgcgc gaattgggcg tggctttggg tttaccacgc
     2821 gaaatggtat accgccaccc attcccaggc ccaggcttgg gcgtgcgcat cttgggcgaa
     2881 gtgaaagccg aatacgccga tttattgcgc cgtgctgacg atattttcat ccaagaattg
     2941 cgcaatttca aagacgaaca aggcgtgtct tggta
//
LOCUS       DAOMVK010000281         2968 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_9843_length_2968_cov_2.742190, whole genome shotgun sequence.
ACCESSION   DAOMVK010000281 DAOMVK010000000
VERSION     DAOMVK010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9843_length_2968_cov_2.742190"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PK856_08935"
     CDS             complement(<1..554)
                     /locus_tag="PK856_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HRL02530.1"
                     /translation="MAWTHDQMAQRAAKELQDGFYVNLGIGLPTMVANHIPEGVDVWL
                     QSENGLLGIGEFPTEATIDPDLINAGKQTVTARPGASFFSSSESFAMIRGGAVNLAIL
                     GAMEVAGNGDLANWMIPGKMVKGMGGAMDLVGGVQRVIVLMEHCTKKGDSKILPACTL
                     PLTGKAVVNRIITDLGVLDVTPEG"
     gene            complement(566..1267)
                     /locus_tag="PK856_08940"
     CDS             complement(566..1267)
                     /locus_tag="PK856_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HRL02531.1"
                     /translation="MNKLYPDAASALADVIQDGQTLAVGGFGLCGIPEALIAALRDTG
                     KKDLTCISNNAGVDGFGLGVLLETRQVKKMISSYVGENKEFERQYLSGELEVELTPQG
                     TLAEKLRSGGAGIPAFYTRTGVGTQIADGKEVKVFNGQEYILEETLVADIALVKAQKA
                     DKAGNLVFNKTARNFNPECAMSGRITIVEVEEIVEIGDLDSDHIHLPGIYVQRIVLNA
                     HPEKRIEKRTLRDAE"
     gene            complement(1473..1607)
                     /locus_tag="PK856_08945"
     CDS             complement(1473..1607)
                     /locus_tag="PK856_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02532.1"
                     /translation="MQHRRRMQNFQALLQHTNRRQLAEKNQQQQPAQPIESHLVSSGR
                     "
     gene            complement(1715..2587)
                     /locus_tag="PK856_08950"
     CDS             complement(1715..2587)
                     /locus_tag="PK856_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009116818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRL02533.1"
                     /translation="MWKPYQQFAQQHFPELCLARPVDSHKGTFGTVAIIGGSTGMSGA
                     AIMAARAALKLGAGKVKIGFAQAALPLPVLEQYPEIMLYTAQELLQHSDISAWSVGCG
                     LDTDASALQLMQQAQQRFQLEPSSKVVWDADALNLFAQHPNLQAAIQPHHIITPHPSE
                     AARLLYQSTSEIQRNRQAAVIQLSERYACISTLKGHHSLVKIPNQAVYENLSGNPGLA
                     TAGSGDILSGFMTSLLAQGINPAIAASAAVWIHGAAAEILALQNIGPIGMVATELVDA
                     ARLLRNQLTLAIDF"
     gene            complement(2781..>2968)
                     /locus_tag="PK856_08955"
     CDS             complement(2781..>2968)
                     /locus_tag="PK856_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003785114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HRL02534.1"
                     /translation="WGEDGSEPNWLNLQQQASSHLHLYGKKSARKGRKMGHFTTLADS
                     ADAALQAAQTLHASLKI"
BASE COUNT          736 a          702 c          707 g          823 t
ORIGIN      
        1 aagccttctg gtgtcacatc caacacaccc aaatcggtga tgatgcggtt tactacggct
       61 ttgcctgtta agggcaaagt acacgcaggc agaattttag aatcgccttt tttggtgcag
      121 tgttccatca acacaatcac gcgttgcacg ccgcccacca agtccatcgc gccgcccatg
      181 cctttgacca tcttgccagg tatcatccag ttggccaaat cgccattgcc agccacttcc
      241 atcgcaccca aaatcgccaa attcaccgcg ccgccgcgta tcatggcaaa cgattctgag
      301 ctagaaaaga aagacgcacc agggcgagct gtcacggttt gtttgcccgc gttaatcaaa
      361 tcagggtcta tggtggcttc agtagggaat tcgccaatgc cgagtaaacc attttccgat
      421 tgcaaccaca catccacgcc ttcagggatg tggttggcca ccatggtggg caagcctatg
      481 cccaaattca cataaaaacc gtcctgtaac tctttggccg cgcgttgcgc catttgatca
      541 tgtgtccaag ccattgttat tctccttatt cagcgtcgcg caaagtgcgt ttttcaatgc
      601 gtttttcagg gtgggcattt aacacgatgc gttgtacata aatgccaggt aagtggatgt
      661 ggtcggaatc caaatcaccg atttccacaa tttcttccac ttcaacaatg gtgatgcgcc
      721 cgctcatggc acattctgga ttgaagttgc gtgcggtttt gttgaatacc aaattgcctg
      781 ctttatcggc tttttgggct ttaaccaagg ctatatcggc taccaaggtt tcttccaaga
      841 tgtattcttg accgttaaac actttcactt ctttgccatc ggcaatttga gtgcccacgc
      901 ctgtgcgggt gtaaaaagca ggaatgccag cgccgcctga gcgcaatttc tcagccaaag
      961 tgccttgtgg cgtcaattcc acttccaatt cgcccgacag gtattggcgc tcgaattctt
     1021 tattttcacc gacataagat gaaatcattt ttttcacttg gcgggtttcc aataacacgc
     1081 ctaagccaaa accgtccacg ccggcgttgt tgctgatgca agtgaggtct tttttgcccg
     1141 tgtcacgcaa agccgcaatc aaggcttctg gaatgccaca caagccaaag ccacccacgg
     1201 ccaaggtttg accatcttga atcacatcag ccaatgcact ggctgcatcg ggatacagtt
     1261 tgttcattct ttttcctttg tacacataag tcttgcttaa atgatgtgag ttgcatattg
     1321 atattgcagc acacaatccc ttgaacatta acattgaatg cataaaaaat aaagttcatg
     1381 cttaagggaa atagcctgtc tcaatgacaa aataaaagcc attcaaatga atggcattgt
     1441 ttgtaatgaa atgaattgct gctgtggttt gcttaacgac cgctgctgac caaatgagat
     1501 tcaatcggct gggctggttg ctgctgctga tttttctctg ccaattggcg gcggttggtg
     1561 tgctgcaata aagcttggaa gttttgcatt ctgcgacggt gttgcatgat tcagtccttt
     1621 cgtggttatt atcattaggt tatacaatga agccaatatc ggtatattag cttaatagtg
     1681 tgacaaaaaa atgaaacaaa agttcaatcg aaaattaaaa gtcaattgcc aaagttaatt
     1741 gatttctcaa taaacgggct gcatccacca gctctgtggc taccatacca ataggaccga
     1801 tattttgtaa agccaaaatt tcagcagctg caccatgtat ccataccgct gcagaggctg
     1861 ctatggctgg attgatgcct tgagccaata gacttgtcat aaatccagac agaatatcgc
     1921 cgctgcctgc tgtggccaaa ccaggattgc cgctcaggtt ttcatataca gcttgattgg
     1981 gtattttgac caagctgtgg tggcctttga gcgtgctgat gcaagcgtaa cgctcactta
     2041 attgaatcac cgctgcttgg cgattgcgtt gaatctcaga agtagactgg tacagcaaac
     2101 gcgccgcttc gcttgggtgg ggtgtgatga tgtgatgagg ctgaatagcg gcctgtaagt
     2161 taggatgttg agcaaacaga ttgagtgcat cggcatccca aacaaccttg ctcgatggct
     2221 ctaattgaaa acgctgttgg gcttgctgca tcaattgcag tgctgacgca tctgtgtcca
     2281 agccgcaacc aacagaccaa gcactgatgt cggagtgttg cagcaattct tgcgcggtgt
     2341 aaagcatgat ttcggggtat tgttccagca ctggcaatgg caacgctgct tgcgcaaagc
     2401 caattttgac tttgcctgca cccagtttca atgcggcccg cgcagccatg atggcagcgc
     2461 cactcatgcc tgtgctgccg cctatgatgg caaccgtacc gaaagtgcct ttgtggctgt
     2521 ctacaggccg agctagacac aattcaggga agtgttgctg ggcaaattgt tggtagggtt
     2581 tccacatggg gattccttgc tgagtgggct taagcatgtt gtgatgacgg cttgcagtgt
     2641 ttggtggtga gtgccatcag tataaagcac acaaatgaat tgtttacatg tacatgcggc
     2701 ctgtaaacca aatatgtttg ggtgaatggc aaaattaaag cagccttatg gctgctttaa
     2761 atgggtgttg aaatcggtat ttatattttc aagctggcat gcaaggtttg agcggcttgt
     2821 aaagcggcat ctgcagaatc tgccaaggtg gtgaagtggc ccatttttcg gcctttacga
     2881 gccgattttt taccatacaa gtgcaaatgg ctactggctt gttgttgcag attgagccag
     2941 tttggttcgc tgccatcttc gccccaca
//
LOCUS       DAOMVK010000282         2961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_9887_length_2961_cov_2.988988, whole genome shotgun sequence.
ACCESSION   DAOMVK010000282 DAOMVK010000000
VERSION     DAOMVK010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9887_length_2961_cov_2.988988"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..557)
                     /locus_tag="PK856_08960"
     CDS             complement(99..557)
                     /locus_tag="PK856_08960"
                     /inference="COORDINATES: protein motif:HMM:NF014766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02535.1"
                     /translation="MYTSVDTLIVGIPTIRPHKLSVTTMQVQTLVLVKITTEDGLVGW
                     GEATTIGGLNYAAESPESVKANIEHYFAPLLLQDKSDNVAQTLKSIHKAINGNRFAKC
                     AVQTALLDIQAQRLGVPLSELLAAVCVTACLCCGLWHRATPTKTLPKPKK"
     gene            667..1581
                     /locus_tag="PK856_08965"
     CDS             667..1581
                     /locus_tag="PK856_08965"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRL02536.1"
                     /translation="MELRHLRYFVAVAEEQSFTKASEKLFIAQPPLSRQIQNLESELG
                     IQLFERGSRPLQLTDAGQFFYQHAVTLLAKAEEVKTMTKRIGLIETTIRMGFVGSLLY
                     GLLSRIVYAFRQQQPQLKIELIEMGTAEQIQALKEGRIDVGFGRLRISDSAVSRVLLR
                     EERLILATHTSHPWASLSEGKYLADVIDQNLLLYPNASKPNFSTQILGLFSEHGLLPK
                     HTKVVREIQLALGLVAAGEGVAIVPESAKAISIPGLHYTPLLDSDAVSPIFLATRSMD
                     DSDYIHSLFDCIYRIYDKEGIPYQRYQS"
     gene            complement(1916..2914)
                     /locus_tag="PK856_08970"
     CDS             complement(1916..2914)
                     /locus_tag="PK856_08970"
                     /inference="COORDINATES: protein motif:HMM:NF012945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02537.1"
                     /translation="MKTLNIAITGFGNVGQHIARLLWQRQAAYRERYATDVRLVGVCR
                     SRSGLLGTQGLNESDVLGAEFVPDLSGEAFLQGLQADVLIDAGPSDFETGGMGLVYMR
                     HALNTGMHVIAVSKGALALHGPELLQTATAHGVQVWVSGATAAALPTVDFVQRQLAGC
                     EVLEIEGIFTGTANFILNQMVHAGMGFDEALQQAQAAGIAEADSRFDTEGWDTAAKIT
                     IIANTLFGTALNVHSLPRQGITDLQAADMATWQAQGLMPRLIGYVRCEQGQVRAGVEV
                     RALDPQEVFYHVQGKNKAMRVVTAEMGELCVQGGASSPQATAAAALKDLENLLKTW"
BASE COUNT          711 a          821 c          667 g          762 t
ORIGIN      
        1 gcccaaggct tgcttgatgg caatcacatg ttccacatct tgctgccatg gattggcacc
       61 gattttgagt ttgaaggtgt tgtgacgctt ggctgcaatc attttttggg cttcggcaat
      121 gtctttgtcg gtgtcgcccg atgccaaagt ccacaacaca ggcaagctgt cacgcaaacg
      181 gccgccaaca attcgctcaa tggcacaccc aagcgctgtg cttgtatgtc gagcaaggcg
      241 gtttgtaccg cgcatttggc aaaacgattg ccgttgatgg ctttgtggat ggatttcaag
      301 gtttgcgcca cattgtcgct tttgtcttgc aacagcaatg gggcgaaata atgttcgatg
      361 ttggctttga cgctctcagg gctttcagcg gcatagttca ggccgcctat ggtggtggct
      421 tcaccccaac cgaccaagcc gtcttcggtg gtgattttca ccaacaccaa ggtttgcact
      481 tgcatggtgg tcaccgacaa tttgtgcgga cgtatggtag ggatgcctac gattaaagtg
      541 tctacggatg tgtacatttt ttcactttca atcttgtttg attgctctgt ttgatatacc
      601 ttaaaagaat gaattacata tgtctaagat taaatttgta tttttctata cttaaaaggt
      661 attgatatgg aattacggca tctgcgctac tttgtggccg tggcagagga gcaaagcttt
      721 accaaagcct cagaaaaatt gttcatcgca cagcctcctt tgagtcggca aattcaaaat
      781 ttggaatctg aattgggcat acaattgttt gaacgcggca gtcgaccttt gcagctcacc
      841 gatgcaggtc aattttttta ccaacatgcc gtcaccttat tggccaaagc cgaagaggtg
      901 aaaaccatga ccaaacgcat aggcttaatc gaaaccacca tccgcatggg ttttgttggc
      961 tctttgcttt atggtttgct gtcgcgcatt gtgtatgcgt ttcgccagca acagcctcag
     1021 ctcaaaattg agctcatcga aatgggcact gccgaacaaa ttcaagccct caaagaaggc
     1081 cgcattgatg tcggctttgg acgtttgcgc atttctgatt ctgccgtcag tcgggttttg
     1141 ctgcgtgaag aacgtttgat tttggccaca cacaccagcc atccgtgggc cagtctgtct
     1201 gagggcaaat acttggccga tgtcattgac caaaatcttt tgctctaccc caatgcctcc
     1261 aagcccaatt tctccaccca aatattgggt ttgttcagcg agcatggcct gctgcccaaa
     1321 cacaccaaag tggtgcgtga aatccaattg gccttaggtc tggtggccgc aggtgaaggc
     1381 gtggccatcg tgccagaaag cgccaaagcc atctcgatac ctggtttgca ttacacgcct
     1441 ttgctcgaca gtgatgcggt cagccccatc tttctcgcca cccgcagcat ggacgacagc
     1501 gactacatcc actccttgtt tgactgcatt taccgcattt atgataaaga aggcattcct
     1561 taccaacgct accaatcgta accgagcata gctttaacaa tgcgtacaga gcaaatgctt
     1621 tcatcgctgt ttttaaagcg ctcttttagg acatgagtat ttatttagat gtcacctatc
     1681 tgcgcatgta ttgctgttat cttatttgtc caagcaaaac aggcagtgtt caataacatc
     1741 caaactgtca gctgttttta aacattcccc ccctttttaa aacaaaaaaa gcggcctgta
     1801 agccgcttca ttaaaaaagt catcccaaca ttgagcctca taccgtgtta tggagaaacg
     1861 ctcgtgcccc cacgctccaa cacactgctc aagctcacca gccaagccag cctgctcacc
     1921 atgttttaag caaattctcc aaatctttca aagccgccgc cgccgttgcc tgcggcgagg
     1981 atgcaccacc ttgcacacac aattcaccca tttccgccgt caccacccgc atcgccttgt
     2041 ttttgccctg cacatggtaa aacacttctt gtggatctag tgcccgcact tccacgccag
     2101 ccctcacttg cccttgttca caacgcacat agccgattaa acgtggcatc aagccttgcg
     2161 cttgccatgt ggccatgtca gcggcctgta aatcggtaat gccttgtcgt ggcaaactgt
     2221 gcacattcaa cgccgttcca aacaaagtgt tggcgatgat ggtgattttg gcggcggtgt
     2281 cccagccttc ggtgtcaaaa cggctgtcgg cttcggcgat gccagcggcc tgcgcttgct
     2341 gcaatgcctc atcaaaaccc atgcccgcgt gcaccatttg attgagaatg aaattggccg
     2401 taccggtgaa aatgccctca atttccaata cctcacagcc tgccaactgt ctttgcacaa
     2461 aatccaccgt gggcaaagcc gctgccgtcg ccccgctcac ccacacttgc accccatgcg
     2521 cggtggctgt ttgcaacaat tccggcccat gcaaggccaa agcccctttg gatacggcga
     2581 tgacgtgcat gccggtgttc aaggcatggc gcatatagac caaacccatg ccaccggttt
     2641 caaaatcgct tgggcctgca tcgattaaca catcggcctg taagccttgc aaaaatgcct
     2701 cgccgctcaa atcaggcaca aattcggcgc ccagcacatc gctctcgttc aagccttgtg
     2761 tgccgagcaa gcctgagcgc gaacggcaca cacccaccaa acgcacatcg gtagcataac
     2821 gctcgcgata cgccgcttgg cgctgccaca gcaaacgcgc aatgtgttga cccacgttgc
     2881 caaagcctgt gatggcgatg ttcaatgttt tcatgcaaac accctcaatt gatctgaatc
     2941 accaacataa acgcagacaa g
//
LOCUS       DAOMVK010000283         2958 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_9895_length_2958_cov_2.901137, whole genome shotgun sequence.
ACCESSION   DAOMVK010000283 DAOMVK010000000
VERSION     DAOMVK010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9895_length_2958_cov_2.901137"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..2030)
                     /locus_tag="PK856_08975"
     CDS             complement(66..2030)
                     /locus_tag="PK856_08975"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HRL02538.1"
                     /translation="MSILSQWQIQQPSSKYAQVMALLTLLWIALPLLQQLPIGVSLLF
                     GVLMLARLGLTMLGIGGLPTWLNIVLMFVAAAAVYGQLQTIFGQEGGVSLLLLMAVIK
                     SFEGKTTRDWQVLLVAALFLVAGGLLFQQTLQIGVWLVLALLMIFSSLCLLNGMGWQL
                     AWRNTMWALGLSLPLMVALFLLVPRLPEPLWRLPTQQNTSKTGVSTELTMGDMAQVIE
                     SNELAFNVTFKQGTLPQTNQLYWRVMVMNEFDGTTWRAGTQQTLLQDALKLTGRPLSY
                     EITLQDFEGHIPALEQVTQVDDSMQVRENQVITAKRPYAQLRKVALQSVLSPTIAERL
                     NPGQKRHWLNLPAHGNLRSRDLARSWKSSSQDDAEVVNQALSFFQTKGFSYSLSPGKL
                     QQNGDQIDEFLFDSKVGFCEHYASSFVVLMRAAGIPARVVTGYLGGTFNDNGQFLQVR
                     GKEAHAWAEVWLQSKQAWVRIDPTSVVSITRIDDGINAALPEQMSAVEGMMPAGLQKW
                     MASSQYYWQQWVVNYDADNQQALFARLGMGALTVTKWLLLAAVVIVLTVSPIIWWWRR
                     QYRQRIEPLSAGFMLIKQHFFHEDQDERWHYGAQDLQQWLQTQQLLTPTWQNLLQQYN
                     NLRYAQIQAPADISWQWYRQVKKALKSKKR"
     gene            complement(2030..>2958)
                     /locus_tag="PK856_08980"
     CDS             complement(2030..>2958)
                     /locus_tag="PK856_08980"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRL02539.1"
                     /translation="QKGLMDTQEAVSWQHLSIRPTQLGINLVLIAAAIWVAALNYAVN
                     LAYGLAFWILSATAVAALLSIRQLLGLRLDIKMPNEVFAGELAEIDIALDAKDLRSRM
                     LIVRFIEEEGAKHSLSKQDLNTLHTHGQGSGRTVLHVQTSHRGYEHLPWLQLISTAPF
                     GLLEVSVVCECQETFVVYPAPQEHTVSVSGRQVAEDAGHSRKVGGDEVAYLKTYEEGQ
                     SLQTIAWKQYAKTGTLLSKHFEEPMASRPDVISYKDYPQVRSPDQLASLLCHRVLEQS
                     TQQQHYVLELPSQVITPQSQQRSLCLNALAVM"
BASE COUNT          801 a          812 c          610 g          735 t
ORIGIN      
        1 cgtctttggc agcatttccc aatccgatgt aaacatggtt catgttgtct ttcccttaaa
       61 ctgaatcagc gctttttgga tttcaacgcc tttttgactt gtcgatacca ctgccagctt
      121 atgtctgctg gggcttgaat ttgagcataa cgcaagttgt tatattgttg cagtaaattt
      181 tgccaagttg gcgtcagcag ctgttgcgtt tgcaaccact gttgcaagtc ctgagcaccg
      241 taatgccacc gctcatcttg gtcttcatga aagaaatgct gtttaatcaa cataaagccc
      301 gcacttaaag gctcaatgcg ctgtcgatac tgacgacgcc accaccatat gattggactc
      361 actgtcaaca caatgacaac ggcagccaat aacaaccact tggtcactgt cagtgccccc
      421 atacccaaac gggcaaacaa ggcttgttgg ttgtcggcat cataattcac tacccattgc
      481 tgccagtagt attgcgaact tgccatccac ttttgcaaac cagcgggcat cataccctca
      541 accgcactca tctgttctgg taaagcggca ttgatgccat cgtcaatgcg ggtgatggaa
      601 accacagaag tgggatcaat gcgtacccaa gcctgtttgc tctgcaacca gacctcagcc
      661 caagcatgag cttctttgcc tcttacttgt aaaaactgtc cattgtcatt gaatgtaccg
      721 cccaaataac ctgtgaccac gcgcgcaggt atgcctgcag cccgcatcaa caccacaaaa
      781 gatgaagcat agtgctcaca aaaacccact ttgctgtcaa acaagaattc atcaatttgg
      841 tcaccatttt gctgcaattt tccaggtgac aaactgtaag aaaaaccttt ggtttgaaaa
      901 aatgacaaag cctgattcac cacttcggca tcgtcttgac tgctgctttt ccagcttctg
      961 gccaaatcac ggctgcgcaa attaccgtgc gcaggcaaat tcagccaatg gcgtttttga
     1021 ccaggattta aacgctcagc aatggtggga cttaacacac tttgcagagc aactttacgc
     1081 aattgagcat acggtctttt cgcggtgatg acttggtttt cacgcacttg catgctgtca
     1141 tccacttgtg tgacttgctc caaagcaggt atgtgcccct caaagtcctg caaggtgatt
     1201 tcataactca aaggccggcc tgtcagcttt aaagcgtcct gtaacaaggt ttgttgagtg
     1261 cccgcacgcc atgttgtgcc atcaaactca ttcatcacca tcactcgcca atacaattga
     1321 ttggtttgag gtaaggttcc ttgtttgaat gtcacattaa aggccaactc atttgactca
     1381 atcacttgtg ccatatcgcc catggtcaac tcagttgaca cccctgtttt agaggtattt
     1441 tgctgagtcg gcaatcgcca caaaggctct ggtaatcgcg gcaccaacaa gaataaggca
     1501 accatcaaag gcaaggacaa acccaatgcc cacatggtat tgcgccaagc aagctgccaa
     1561 cccataccat tcaacaaaca caagctgctg aaaatcatca acaaggccaa caccaaccac
     1621 accccgattt gcaaagtctg ttggaacagc aaaccacccg caaccaagaa caatgccgcg
     1681 accaacaaca cctgccaatc acgggtggtt ttgccttcaa atgacttgat gaccgccatc
     1741 aacaacagca atgacacgcc accttcttgc ccgaatatgg tttgcaattg cccatacacc
     1801 gcagcagcgg ccacaaacat caacacaatg ttcaaccaag tcggtagacc acctatacct
     1861 agcatggtca aacccagtct ggccagcatc aacacaccaa acaacagact cactccaatg
     1921 ggtaactgct gtaacaaagg caaagcaatc cacaacaagg tcaacaatgc catgacttgt
     1981 gcgtatttgc ttgaaggctg ttggatttgc cattgagaaa gaatgctcat tacatcaccg
     2041 ccaatgcatt caaacacaag ctgcgctgtt gcgactgtgg ggtaatgact tggcttggca
     2101 actccaaaac ataatgctgt tgctgcgtgc tttgctccaa aacccgatgg cacaataagc
     2161 tggccaattg gtcaggcgag cgtacttgcg ggtaatcttt gtaagaaatc acatctggac
     2221 gcgatgccat aggctcctca aagtgcttac tcaataaagt gcccgttttc gcatattgtt
     2281 tccaagcgat ggtttgtaaa gactggcctt cttcataagt cttgagatat gccacttcat
     2341 ctccacccac tttgcggctg tgccctgcgt cttcggcaac ttgtcgccct gacacgctga
     2401 ccgtatgctc ttgtggcgca ggatacacga caaatgtttc ttggcattcg cacaccaccg
     2461 atacctctaa caatccaaat ggggcggtac tgatgagctg caaccatggc aaatgctcat
     2521 accctcggtg tgaggtttgc acgtgtaaaa cagtccgccc actaccttgc ccatgggtat
     2581 gcaaagtgtt caaatcttgc ttgctcaaac tgtgcttggc accctcttct tcaataaacc
     2641 gcacaatcaa catgcggctg cgcaaatctt tggcgtctaa ggcaatgtct atttcagcca
     2701 attcgcctgc aaacacttca ttgggcattt tgatgtccaa gcgcaaaccc agtaattgcc
     2761 gtatgctcaa cagcgccgcc acagccgtgg cgcttaaaat ccaaaaagcc aagccatagg
     2821 ccaagttgac cgcataattc aatgcggcca cccatattgc ggcggcaatc aacaccaaat
     2881 tgatgcccaa ctgagttggg cggatggata agtgctgcca agacacggct tcttgcgtgt
     2941 ccattaagcc tttttggc
//
LOCUS       DAOMVK010000284         2953 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_9918_length_2953_cov_2.524155, whole genome shotgun sequence.
ACCESSION   DAOMVK010000284 DAOMVK010000000
VERSION     DAOMVK010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9918_length_2953_cov_2.524155"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PK856_08985"
     CDS             complement(<1..213)
                     /locus_tag="PK856_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009115660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HRL02540.1"
                     /translation="MMDASNLVWLDLEMTGLDADKERIIEAAMIVTDTNLNILAQSPI
                     WVVHQSDALLAAMDEWNTATHTRTGLV"
     gene            334..1584
                     /locus_tag="PK856_08990"
     CDS             334..1584
                     /locus_tag="PK856_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRL02541.1"
                     /translation="MLTGLFLLFFVLSILIQLYLSVRQSLAVRSHRSFVPEAFTSQVT
                     LEEHHKAADYTLAKQRLHRYEILFQAALLLFWTLAGGLNVIAAIAIKWLPNTPILQGV
                     VLIGLFSLISGILGLPFALYRTFKLESRFGFNRSTLATFISDQFKGVFIATLIGAPLL
                     YLIIYLMGAMGNTWWLSVWLVWAGFSLLLMWAFPKWIAPLFNKFEPMPEGGLKTRIEA
                     LLTRTGFHSNGLFVMDGSKRSGHGNAYFTGLGQQKRIVFFDTLLKDLSDSEVEAVLAH
                     ELGHFKHGHVFKQIAISLGLGLVVLYALGALLNLPDFAFFTQLGVDYPSHAMALLLFM
                     LVLPVFTFPFSPLSSIMSRKNEYEADRFAAHNSNAIDLISALTKLYRSNASTLTPDAW
                     YSRFYDSHPNASERIAALQQAGKR"
     gene            1584..2462
                     /gene="rsgA"
                     /locus_tag="PK856_08995"
     CDS             1584..2462
                     /gene="rsgA"
                     /locus_tag="PK856_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRL02542.1"
                     /translation="MQIAQIITSYGRLYIVRDEQGQTFQATTRSKRVDYACGDHVHIQ
                     SINAEQVVIEDFLERSSLLYRQDAFKSKLIAANVTQLLIVVAAEPSPNETLVQRALLA
                     AEAAHIEAHIVLNKADLDGTAKWQPVFQFYADMGYGYSSLSALDGADALRPILQGHSN
                     IFLGQSGMGKSTLTNAILGENIARTGDISTALDSGKHTTTHAQLYQLDADTQLIDSPG
                     LQAFGLHHLERAETALTDLLHYFPDLRHLIGQCRFHNCTHRNEPNCAVKNAAESGDIA
                     PHRLHFLQTITDELSH"
BASE COUNT          724 a          848 c          626 g          755 t
ORIGIN      
        1 aaccaagccc gtgcgggtat gggttgctgt attccactca tccatggcag ccaacaaagc
       61 atcgctttgg tgcaccaccc aaatcggaga ttgggccaag atgtttaaat tggtgtcggt
      121 cactatcatg gcggcttcga taatgcgctc tttatcagca tccaaacccg tcatttccaa
      181 atccaaccac accaaattac ttgcatccat cattgtcttt tccttattta agttggctat
      241 tttgccgcag ccattcgagc atgacaagca atgcgcatga ggctgggcaa atcatgcgat
      301 aatgccaaca ctttcatttt ggcacatcag cccatgctta ctggattatt tttattgttt
      361 tttgtgctca gcatcttgat tcaattgtat ttgtcggtac gccaaagttt ggcggtacgc
      421 agccaccgca gctttgtgcc agaggctttt acctcacagg tgactttaga agagcaccac
      481 aaagccgcag actacacttt ggccaaacag cgtttacacc gttatgaaat tttgtttcag
      541 gccgcattat tgttgttttg gacactggca ggcggcctga atgtcatcgc cgccatcgcc
      601 atcaaatggc tgcccaatac ccctattctg caaggtgtgg ttttgatcgg cttgttcagc
      661 ctcatcagcg gcatcttggg cttgccgttc gcgctgtacc gcaccttcaa attggaaagc
      721 cgttttggct tcaaccgcag caccctcgcc accttcatca gtgaccaatt caaaggcgtg
      781 tttatcgcca ccttaattgg cgcacctttg ctctacctca tcatttacct gatgggtgcc
      841 atgggcaaca catggtggct gtctgtgtgg ctggtgtggg caggcttctc cttattgttg
      901 atgtgggcat ttcccaaatg gattgcgcct ttgttcaaca aatttgaacc catgcccgaa
      961 ggcggcctga aaacccgcat cgaagccttg ctcacccgca caggctttca cagcaacggt
     1021 ttatttgtga tggacggttc caaacgctca ggccacggca atgcttattt cacaggtttg
     1081 ggccagcaaa agcgcatcgt gtttttcgat accttattaa aagatttaag cgacagtgaa
     1141 gtcgaagccg tcttggcgca cgaattgggc catttcaaac acggccatgt gttcaaacaa
     1201 atcgccatca gtttgggact gggcttggtg gtgttgtatg cattgggtgc tttgttaaac
     1261 ctgcctgatt ttgctttctt cacacaattg ggcgtggact accccagcca tgccatggct
     1321 ttactgctgt ttatgttggt attgcctgtg ttcacgtttc cgttttcacc gctgtccagc
     1381 atcatgtcgc gcaaaaatga atacgaagcc gaccgctttg ccgcacacaa cagcaatgcc
     1441 atcgacttga tttccgcctt aaccaaactc tatcgcagca atgccagcac cttaaccccc
     1501 gatgcttggt attcacgctt ttacgattca caccccaatg ccagcgaacg catcgccgca
     1561 ttgcagcaag caggaaaacg ctgatgcaaa tagcacaaat catcaccagt tacggccgcc
     1621 tttacatcgt gcgcgacgaa caagggcaaa cctttcaagc caccacccgc agcaagcgcg
     1681 ttgactatgc ctgcggcgac catgtacaca tccaatccat caatgctgag caagtggtga
     1741 ttgaggattt tttagagcgc agcagcctgc tctaccgcca agatgccttc aaaagcaaac
     1801 tcattgccgc caacgtcacc caattgctca tcgtggtggc cgccgagcct tcaccgaatg
     1861 aaaccttggt acaacgcgcc ttgcttgccg ccgaagccgc ccacatcgaa gcacacattg
     1921 tattgaacaa agccgatttg gacggcacag ccaagtggca accggtgttt cagttttacg
     1981 ccgacatggg ctatggctac agcagcctca gcgccctcga cggtgcagac gctttgcgcc
     2041 ccatactgca aggtcacagc aatattttct taggccaatc gggcatgggc aaatccacgc
     2101 tgaccaatgc catcctgggt gaaaacattg cccgcactgg cgatatttcc accgctttgg
     2161 attcgggcaa acacaccacc acccatgccc agctctacca actcgatgcc gacacgcaat
     2221 tgattgattc cccaggtttg caagcctttg gtttacacca cttagaacgc gctgaaacgg
     2281 cactgaccga tttgctgcat tacttccccg atttgcgcca tttaatcggt cagtgccgtt
     2341 tccacaattg cacccaccgc aacgagccca attgcgccgt gaaaaatgcc gccgaaagcg
     2401 gcgacattgc cccgcaccgt ttgcacttct tgcaaaccat cactgatgag ctgtcacact
     2461 aacgctcaaa cacctcaaaa gcggcctgta actcttttac aggccgcttt tattgggttt
     2521 taagtaaatg tgtcatactt gatgtgatgt cagagattaa agtccaacaa tatttaaccc
     2581 cgtcccatgc ctgccgaagg gtcagccaac agcctcaacc tgactcaatt caaacacata
     2641 ccgcactcag aatttatctc cttacaagcc tggattttgt tccaaaccct caaccaacgg
     2701 cttgaaacaa accacattca acacaccacc aaatcacaac tacttacaca agccgccttt
     2761 accccattca caaaccatca acaaaagcat acaaaagcgg cctgtaaatg cttacaggcc
     2821 gcttaaacat ccgctaaggc ttattgagcc gctacaggtg cttcagggat ttccaagcca
     2881 ttggctttga acaaagcctt caattgctct tctactttgg catttttcgc ttcagcagcc
     2941 aattggtctt tga
//
LOCUS       DAOMVK010000285         2947 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_9942_length_2947_cov_2.871369, whole genome shotgun sequence.
ACCESSION   DAOMVK010000285 DAOMVK010000000
VERSION     DAOMVK010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9942_length_2947_cov_2.871369"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="PK856_09000"
     CDS             <1..783
                     /locus_tag="PK856_09000"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HRL02543.1"
                     /translation="AAAGIMLATAGVILQRMTLNPLASPELLGISAGTAAGVFVAVLL
                     GISAGVQVWLCGIAAAITTLAAILFVNRNSGLQPERILLTGMAIAALFDALINVFLAA
                     GDPRAQAVLVWLSGSTYQATPLLANTLIVLAVCLLVLSLPLARCLGLLSLGSPMAQAL
                     GLNVALTQKGLVVLAAILTALSTLMLGPMCFVGLWAPHLAKFLGFHKPLHQLLAAAAI
                     AVIAMVFADWLGRQIVYPYEIPAGLMATLLGGAYFFRLMRKI"
     gene            780..1244
                     /locus_tag="PK856_09005"
     CDS             780..1244
                     /locus_tag="PK856_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02544.1"
                     /translation="MNVTSTLSFAFKRYRHSMAVVLLLSLLASAMGIGVLAYINTRLL
                     QADAGFVHAIWWFVLLLALYLLVATAAQMALTRLGHHLVYDLLTRLLKQIMDSDLRHI
                     QTWARAKSWPAWPVTFKPSAICLCACPNCCKVACSLPQPACICCICRPACLP"
     gene            1160..2425
                     /locus_tag="PK856_09010"
     CDS             1160..2425
                     /locus_tag="PK856_09010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic peptide export ABC transporter"
                     /protein_id="HRL02545.1"
                     /translation="MPELLQGGLFIAAASVYLLYLSPSLFAVTLLWIAATLVGGYVTV
                     KHVYRHLGHMRSQENDLFQQYEAAITGHKELSLNRARAQRFYQESFTPTAQKRRLHVI
                     WADNFHALAGNWTNVMMLGAVGFIFYLAYYRHWASIADATTIAITVLFIRTPLLAAVG
                     AYPTLLQGKVALRALQDLRLPEPSPDFLQAASLPHNWQTIAFKQVTYAYQEAQTADAS
                     FHLSPINFSLNRGETVFLVGKNGSGKSTLSLLLSGLYLPHSGHISVDGVAIDEHNRSA
                     YRQYFSSVFTDFHLFSQLLGGDGALADADLVSAWVAHLHLQDKIHIENSHIQNTALSQ
                     GQKKRLGLLLAAVEQRPVLILDEWAADQDPHFRKVFYEQLLPVLKAQGHTIFAISHDD
                     KYFHHADRIIQMHNGKLSDFDAASADKSF"
     gene            2447..>2947
                     /locus_tag="PK856_09015"
     CDS             2447..>2947
                     /locus_tag="PK856_09015"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRL02546.1"
                     /translation="MSYSKLPKFHLALLSTAVLLAYSQVQAADEVADSAEADTELEAI
                     TVVGSAGKIGGIKFHDTRSADVIKRNNMDENAIHKIDEAIANQAGVLTNMYGTDNKVD
                     WFKIRGFDASTAIDGTPTTPNGFFVWLPEAYGVESVEVIKGANSNLYGASGAGGVVNL
                     VSKRPKS"
BASE COUNT          627 a          843 c          740 g          737 t
ORIGIN      
        1 gctgccgcag gcatcatgct cgccacagcg ggggtgattt tacagcgcat gacgttgaac
       61 ccactggcca gtcccgaatt gctcggcatc agcgcaggca ctgccgcggg ggtgtttgtg
      121 gcggtattgc tgggcatcag cgcaggggtg caagtgtggc tgtgcggcat cgccgctgcc
      181 atcaccacgc tggctgccat cttgtttgtc aaccgcaaca gcggcttgca acccgaacga
      241 atcttgctca caggcatggc catcgccgcc ttattcgatg ccctcatcaa tgtgtttctc
      301 gccgctggcg accctcgtgc gcaagcggta ttggtgtggc tgtctggctc cacctaccaa
      361 gccacgccct tgcttgccaa caccttaatt gtgctggccg tgtgtttgtt ggtattgagt
      421 ttgcctttgg cacgctgctt gggcttgctc agtttgggca gccccatggc acaggccttg
      481 ggcttgaatg ttgcccttac ccaaaaaggc ttggtggtgt tggccgccat cttaacggcc
      541 ttatccacgt tgatgctcgg ccccatgtgt tttgtcggcc tgtgggcacc ccatcttgcc
      601 aaattcttgg gctttcacaa gccattacac caattgctcg ccgcggctgc catcgccgtg
      661 attgcgatgg tgtttgccga ttggctgggc cgccaaattg tttatcccta tgaaattcct
      721 gcaggcttga tggccacatt gcttggcggt gcttacttct tcagattgat gcgaaaaata
      781 tgaatgtgac ttccaccctg tcttttgcct tcaaacgcta tcgccacagc atggctgtgg
      841 ttttgctgct gtcgctgctc gcaagcgcaa tgggcatagg cgtattggct tacatcaaca
      901 cgcgtttgtt acaggccgat gctggctttg tgcatgccat ttggtggttt gtactgttgc
      961 tcgccctgta cttattggtg gccactgccg cgcaaatggc cttaacccgc ttgggccacc
     1021 atctggtcta cgacttactc acgcgcttgc tcaagcaaat tatggacagt gatttgcgcc
     1081 acatccaaac ttgggcaagg gcaaaatcat ggccagcttg gccggtgaca ttcaagccat
     1141 cagctatatg tttgtgcgca tgcccgaatt gttgcaaggt ggcctgttca ttgccgcagc
     1201 cagcgtgtat ttgctgtatt tgtcgcccag cttgtttgcc gtgaccttgc tgtggattgc
     1261 cgccaccttg gtcggtggct atgtcaccgt caaacacgtt taccgccact tgggccacat
     1321 gcgcagccaa gaaaacgact tgttccaaca atacgaagcc gccatcactg gccacaaaga
     1381 gctgagctta aaccgcgccc gtgcgcaacg cttttaccaa gaaagcttca cccccaccgc
     1441 gcaaaagcgt cgcctccatg tgatttgggc cgacaatttt cacgcactgg caggcaattg
     1501 gaccaatgtg atgatgttgg gtgcggtcgg ctttatcttc tatttggcct attaccgcca
     1561 ctgggccagt atcgccgatg ccaccaccat cgccatcact gtgttgttca tccgcacgcc
     1621 tttgctggcg gctgtgggcg cttaccctac tttattgcaa ggcaaagtgg ctttgcgggc
     1681 tttacaagat ttgcgcttgc ctgagcccag cccagacttt ttacaggccg catctttgcc
     1741 gcacaactgg caaaccatag ccttcaagca agtgacgtat gcttatcaag aagcccaaac
     1801 agccgacgca tcgtttcatt tgtcaccgat taatttcagt ctcaaccgcg gcgaaaccgt
     1861 gtttttggtg ggcaaaaatg gcagcggcaa atcgacttta tcactgttat taagcggcct
     1921 gtatttgcct cacagtggcc acatcagcgt cgatggcgtg gccatagacg aacacaatcg
     1981 cagcgcttac cgccagtatt tttccagcgt gttcaccgat tttcatttat tcagccaatt
     2041 gcttggtggc gatggtgcgc tcgccgatgc cgacttggta agtgcttggg tcgcgcattt
     2101 gcacctgcaa gacaaaatcc acatcgaaaa cagccacatc caaaacaccg ccttatcgca
     2161 aggccagaaa aaacgcttag gcctgctgct cgccgcagtg gaacagcggc ctgtattgat
     2221 actggacgaa tgggctgccg accaagaccc acactttcgc aaagtgtttt acgagcaatt
     2281 gctgcctgtg ttgaaagcgc aaggccacac catttttgcc atcagccacg atgacaaata
     2341 tttccaccat gccgaccgca tcatccaaat gcacaacggc aagctgtctg attttgatgc
     2401 agccagcgca gacaaatcat tttaaacccc tccaagaaag cctcttatgt cttattccaa
     2461 acttcccaaa ttccatctgg cactgctcag caccgccgta ttgctcgctt acagccaagt
     2521 acaggctgcc gatgaagtgg ccgactctgc cgaagccgat accgaactcg aagccatcac
     2581 cgtggtgggc tcggctggca aaatcggtgg catcaaattc cacgataccc gttcggccga
     2641 tgtgattaag cgcaacaata tggatgaaaa cgccatccac aaaatcgatg aagccattgc
     2701 caatcaagca ggcgtgttga ccaatatgta tggcaccgac aacaaagtgg attggttcaa
     2761 aatccgcggc tttgatgcct ctactgccat cgatggcacc cccaccaccc ccaatggctt
     2821 ttttgtgtgg ctacctgaag cctacggcgt ggaaagcgtg gaagtcatca aaggtgccaa
     2881 ctccaatttg tatggcgcgt ctggtgcagg tggcgtggtg aatttggtga gcaagcgccc
     2941 caaatct
//
LOCUS       DAOMVK010000286         2939 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_9974_length_2939_cov_3.003814, whole genome shotgun sequence.
ACCESSION   DAOMVK010000286 DAOMVK010000000
VERSION     DAOMVK010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9974_length_2939_cov_3.003814"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /gene="gluQRS"
                     /locus_tag="PK856_09020"
     CDS             <1..773
                     /gene="gluQRS"
                     /locus_tag="PK856_09020"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HRL02547.1"
                     /translation="ILHTLEAFALCWDGEVMYQHQRHDAYQAALETLQAADEVYPCYC
                     SRKDIAALNPVQGVDGWVYPKICAEPLDQSEQAYRPHRDDKTPAWRVRVPDAVYGFDD
                     AIVGHYQQNLAQEVGDFVLKRADGWWAYQLAVVVDDAEQGMSHVVRGQDLLVSTPRQL
                     YLQQLLGLPQPHYAHLPLLTNALGQKWSKQTQAPALDVKHRVALLNQVLDILGLPKRP
                     AHDMTCAELLAWAVPLWAIEKVPSQAHMVDSNADIEPN"
     gene            834..1100
                     /locus_tag="PK856_09025"
     CDS             834..1100
                     /locus_tag="PK856_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02548.1"
                     /translation="MSKLASLIKMVGRNPESKAAAIATAATTGITIAKFLGKRSIYGA
                     IAGVGVSLIAKKLMEKQTQRTAAKDDIIEHAPLQPDPNAPKRGE"
     gene            complement(1162..1854)
                     /locus_tag="PK856_09030"
     CDS             complement(1162..1854)
                     /locus_tag="PK856_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HRL02549.1"
                     /translation="MSFFAQPLWMLFVILAVYQFSLWLRKTTGSVWLPPILTCTVLLI
                     AYLKLFGVDYQYFQKGAEVVDFWLKPAVVVLAVPLYQQRKKIIQLWLPITVSQVLGSI
                     VGLVSGVWLARLFGAADIVAVSLAAKSVTNPIALDITLSLNGIPAITAVGVIFAGLTG
                     QILGFKLLEVLGIKTMSAQGLALGSASHALGINAAWQRGPEYAAYATLGLILNGILSA
                     IIAPYLIPWLFA"
     gene            complement(1851..2195)
                     /locus_tag="PK856_09035"
     CDS             complement(1851..2195)
                     /locus_tag="PK856_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HRL02550.1"
                     /translation="MIKGLLAVAASLATGEAIVYFTHWPLPASVLGLIALYLALTLKW
                     VTVEDLTAITDFLMQHLALFLIPPCVALITHLDLIQADFLPLLGASFISTVLLVLVVG
                     RVAQWLQKGKQS"
     gene            complement(2283..>2939)
                     /locus_tag="PK856_09040"
     CDS             complement(2283..>2939)
                     /locus_tag="PK856_09040"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HRL02551.1"
                     /translation="KNVKGIILRANSPGGSPVISKVAFDEIRRLKAAHKNIPVVVVME
                     DVCASGCYYIASAADTIYANPSSLVGSIGVISGGFGFTGLMDKLGVERRMKTAGANKG
                     MGDPFSPETPEQNAIWQSMLTDIHKQFVDAVKLGRGKKIDETKNPDVFSGRVYTGDQG
                     KQVGLVDEFGSVYTVSRDVFKAPEMVNYTPKDDFSTSFGRQFGMQMKSAADTLTLPSW
                     "
BASE COUNT          771 a          748 c          755 g          665 t
ORIGIN      
        1 atattttgca cacactcgag gcttttgccc tgtgttggga tggcgaagtg atgtaccaac
       61 accagcgcca tgatgcttat caagccgctt tggaaacctt acaggccgct gatgaagtgt
      121 atccgtgtta ttgcagtcgc aaagacattg cggctttaaa ccctgtgcaa ggtgtggacg
      181 gttgggtgta tcccaaaatc tgtgcagaac cattggatca gtctgagcag gcttacaggc
      241 cgcatcgtga tgacaaaacc cctgcttggc gcgtgcgtgt gcctgatgcg gtgtatggtt
      301 ttgacgatgc gatagtcggc cattatcagc aaaatttggc gcaggaagtg ggcgatttcg
      361 tgctcaagcg tgccgatggt tggtgggcat accaactggc agtggtggta gacgatgccg
      421 aacaaggcat gagccatgtg gtgcgtgggc aagatttgtt ggtgtccaca ccgcggcagc
      481 tgtatttgca acagctgttg ggcttgccac agccgcacta tgcacattta ccgcttttaa
      541 ccaatgcctt ggggcagaag tggtctaagc aaacacaggc gcctgctttg gatgtgaagc
      601 accgagttgc cttattgaat caggtattgg acatcttggg tttgcccaag cggcctgcac
      661 acgacatgac gtgtgccgag ttgctggctt gggctgtgcc tttgtgggct attgaaaaag
      721 tgcccagtca ggcgcatatg gtggattcca atgccgacat tgaacctaac tgaaatcccg
      781 ccatctattc aagcaaacat caagcacata aatggcacac aaaggagtgt tgtatgagta
      841 aattggcaag tttgattaaa atggtgggcc gcaatcctga atccaaagcc gctgccatcg
      901 ccaccgctgc caccacaggc attaccatcg ccaagttttt gggcaagcgt tcgatttatg
      961 gcgcgattgc gggcgtgggc gtgagtttga ttgcgaaaaa attgatggaa aaacaaacac
     1021 agcgcacggc ggccaaagac gacatcatcg aacacgcgcc tttgcaacct gatcccaatg
     1081 cgcccaaacg tggcgaataa gcagacccgc attcaagcaa aaaacctcgc aattgcgagg
     1141 ttttttcatg gctaaatcaa atcaagcaaa cagccaagga atcaaatagg gtgcgatgat
     1201 ggcgctcaaa atgccattca aaatcaaacc caaagtcgcg taagccgcat attcagggcc
     1261 gcgttgccag gcggcattga tgcccaaggc atgagacgcc gaacccaaag ccaagccttg
     1321 tgcgctcatg gttttgatgc ccagcacttc cagcaatttg aaacccaaaa tttgccccgt
     1381 taaacccgcg aaaatcacac cgacagcggt gatggcaggg atgccgttga ggctaagagt
     1441 aatgtccaag gcgatggggt tggtgaccga cttggccgcc aaagacacgg ccacgatgtc
     1501 ggctgcgcca aacaagcgcg ccaaccacac accagaaacc aagcctacaa tggaacccaa
     1561 tacctgtgac acggtaatcg gcagccacaa ttgaatgatt tttttgcgct gttggtacaa
     1621 aggcacggcc aataccacca cagcaggttt gagccagaaa tccaccactt ccgcgccttt
     1681 ttgaaagtat tggtaatcca ccccaaacag tttcaaatac gcaatgagta aaacggtaca
     1741 cgttaaaatc ggcggcagcc acacactgcc tgtggttttg cgcaaccaca gtgaaaactg
     1801 gtacaccgcc aaaatcacaa acaacatcca caaaggttgg gcaaagaaac tcatgattgt
     1861 ttgcctttct gtagccattg cgccacacga cccaccacca acaccaataa cacagtgctg
     1921 atgaagctgg cacccaagag cggtaaaaag tcggcctgta tcaaatccaa atgcgtgatc
     1981 aaggccacgc aagggggaat caggaacaag gccaagtgtt gcatcaaaaa gtcggtgatg
     2041 gcagtcaaat cttctactgt tacccatttg agcgttaagg ccaaatacaa ggcgatgagg
     2101 cccaaaacgc tggcaggcaa gggccagtgg gtaaagtaaa ctatggcttc tccagttgcc
     2161 agtgaggcgg caactgccaa taagccttta atcatggtgt gtttctcctc ttcaattctt
     2221 tattctagtg taaaaaagcg gcctgtaagc ttacaggccg ctttgaacag cgattatagg
     2281 gtttaccacg atggtaaggt caaggtgtct gctgcagatt tcatctgcat gccaaattgg
     2341 cggccaaacg aggttgaaaa atcgtctttc ggggtgtagt tcaccatttc aggcgctttg
     2401 aacacatcac gagacacggt gtacacgctg ccaaactcat ccaccaagcc cacttgtttg
     2461 ccttgatcgc ctgtataaac gcggccactg aagacatctg ggtttttggt ttcatcaatt
     2521 tttttgccac ggcccaattt cactgcatcg acaaattgct tgtggatgtc ggtgagcatg
     2581 gattgccaaa tcgcgttttg ctcaggcgtt tcgggcgaga atgggtcgcc catgcctttg
     2641 ttggcgcccg cggttttcat gcgtcgctcg acgccgagct tgtccatcaa gcccgtaaag
     2701 ccaaagccgc ctgaaatcac gcctatgctg cccaccaagc tagatgggtt ggcataaatg
     2761 gtgtcggcag cactggcgat gtaataacag cccgaggcac acacgtcttc catcaccacc
     2821 accacaggga tgtttttgtg ggcggccttg agacggcgta tctcatcaaa cgccactttg
     2881 gaaatcacgg gtgagccgcc agggctgttg gcgcgcaaga tgatgccttt gacgttttt
//
LOCUS       DAOMVK010000287         2933 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_9997_length_2933_cov_3.306810, whole genome shotgun sequence.
ACCESSION   DAOMVK010000287 DAOMVK010000000
VERSION     DAOMVK010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9997_length_2933_cov_3.306810"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="PK856_09045"
     CDS             complement(<1..614)
                     /locus_tag="PK856_09045"
                     /inference="COORDINATES: protein motif:HMM:NF012550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HRL02552.1"
                     /translation="MTTSLNSFQALKQLDVAGKTYHYFDLQAASAQIGDVSRLPKSLK
                     VLLENLLRFEDNVSVKSDDIKAIAAWLQSKSSKQEIQYRPARVLMQDFTGVPAVVDLA
                     AMRQAMQKAGGDANKINPLSPVDLVIDHSVMVDHFASGNAFDLNVKLEMQRNEERYQF
                     LRWGQHAFDNFSVVPPGTGICHQVNLEYLGQTVWTRDVGGNTYA"
     gene            complement(776..1357)
                     /locus_tag="PK856_09050"
     CDS             complement(776..1357)
                     /locus_tag="PK856_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRL02553.1"
                     /translation="MKKTAAGVVGAVALMAAMAAQAAPVTYQFDPDHTYPSFETDHFG
                     GISTWRGKFTQTSGKVVVDTAKKTGELEAVINMDSFDSGNAGLNTHAKGAEILDVAKY
                     PTAVYKGKLTKFKLGKPTEVVGELTLHGVTKPLNLKINSFKCFENPMSKKETCGADAT
                     AKFDRAVFGIDYGKGYGFNMQTTLNIQVEGIRQ"
     gene            complement(1522..2820)
                     /gene="tyrS"
                     /locus_tag="PK856_09055"
     CDS             complement(1522..2820)
                     /gene="tyrS"
                     /locus_tag="PK856_09055"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRL02554.1"
                     /translation="MSGLINELQQRGLIAQTTDAEALQQLLDQEQISLYCGFDPTADS
                     LHIGHLLPVLALRRFQLAGHKPIALVGGATGMIGDPSFKAVERSLNTPETVAGWVEKI
                     RNQLTPFLSFDGDNAATMANNHDWFGGMGCLDFLRDIGKHFSVNAMMNKESVKQRLDR
                     DDVGISFTEFAYSLLQAYDFTQLNSRHACKLQIGGSDQWGNITAGIDLTRRLNKDTVY
                     GLTLPLVTKSDGTKFGKTEGGAVWLDAKKTSPYQFYQFWLKVADADVYKFLKFFTFLS
                     VEEINAIEAADQASGTKPEAQRILAEQMTRLIHGEEALAAAQRISASLFAEDQSDLTE
                     SDFEQLALDGLPSFKAEGGVNVVEALVLTDLAKSNKEGRGFVASGAVALNGAVAVANN
                     PEHAPEKPDDAFMLTEADKRFGKYTIIRRGKKLHALLIWA"
BASE COUNT          748 a          739 c          661 g          785 t
ORIGIN      
        1 aaggcatagg tattgccacc cacatcgcgc gtccacaccg tttggcccaa atactccaaa
       61 ttcacttggt gacaaatgcc cgtacctgga ggcacgacgc tgaaattatc aaacgcatgc
      121 tggccccaac gcaaaaattg gtagcgctct tcattgcgct gcatttccaa tttcacgttc
      181 aagtcaaacg cattgccact ggcaaagtgg tctaccatca ccgagtggtc tatcaccaaa
      241 tccacaggcg acaagggatt gattttattg gcgtcgccac ctgctttttg catggcttgg
      301 cgcatggccg ccaaatccac caccgcaggc acaccggtaa agtcttgcat caacacccga
      361 gcaggtcggt attggatttc ttgcttactg gatttacttt gcagccatgc ggctatggct
      421 ttgatgtcat cagacttgac cgaaacattg tcttcaaaac gcaataggtt ttccaacaac
      481 acttttagag acttgggcaa acggctgaca tcgccaattt gagcagatgc ggcctgtaaa
      541 tcaaaatagt ggtaggtttt acctgccaca tcgagttgtt tcaaagcttg aaagctgttt
      601 aatgaggtag tcataacgat tcctaaatca aagacaactg gttccgagtg tgttttatta
      661 gcagactgtg tgagcacaca aaagttttta tggatggcgt agcttaagac ttaggctatc
      721 acatccataa aaaaacgcac ccatcacagg tacgttttaa cttaatcttg catgcttatt
      781 ggcgtatgcc ttcaacctgt atgttcaatg tggtttgcat attgaaaccg tagcctttgc
      841 catagtctat gccaaacact gcacggtcaa atttggcagt ggcatcggca ccgcaagttt
      901 cttttttgct catcgggttt tcaaaacatt tgaatgagtt gatttttaaa ttcagaggtt
      961 tggtcacacc gtgtaaggtc aattcaccca ccacttccgt gggtttaccc aatttgaatt
     1021 tggtcaactt acccttgtaa accgcggtag ggtatttggc cacatccaag atttctgcgc
     1081 ctttggcgtg ggtgttcaat cccgcattgc ccgaatcaaa agaatccatg ttgatcacag
     1141 cttccaattc acctgttttc ttggcagtgt ctaccaccac tttgccagaa gtttgggtga
     1201 atttaccgcg ccaagtcgaa atgccaccaa agtggtcggt ttcaaaactt ggataagtgt
     1261 ggtctggatc aaactgataa gtcacaggcg ctgcttgagc cgccattgct gccatcaatg
     1321 ccaccgcacc taccacgcct gctgctgttt ttttcatgtt ggtttcctgt aaacgataca
     1381 aattgaaagt gtgcccactg tagtggatat gccatttggc aacaagccga tttaagtaaa
     1441 tgatgcttta ctcagcgtta acaatgcatg aataagcggc ctgtaaacac gttacaggcc
     1501 gcttgaataa cctcagaacg cttatgccca gatcaacaag gcatgcagtt tcttaccacg
     1561 gcgaatgatg gtgtatttgc caaaacgctt gtcggcttcg gtcaacataa acgcatcgtc
     1621 tggtttctca ggcgcatgct caggattgtt ggccacagcc accgcaccat tcaacgctac
     1681 cgcgccactg gccacaaagc cacggccttc tttattggat ttggccaaat cagtcaaaac
     1741 caaagcctcg accacgttca cgccgccttc tgccttgaag ctgggcaaac catccaaagc
     1801 caattgctcg aaatcacttt cagtcaaatc gctttggtct tcggcaaaca agctggcgga
     1861 aatgcgctgc gctgctgcca aagcctcttc gccatggatt aagcgtgtca tttgctcagc
     1921 caaaatgcgt tgcgcctctg gtttggtacc gctggcttgg tcggccgctt caatggcatt
     1981 gatttcttcc acgcttaaga aggtgaagaa tttcaagaat ttgtacacat cggcatcggc
     2041 cactttgagc cagaattggt agaattggta aggcgaggtt ttcttcgcat ccaaccacac
     2101 cgcgccgcct tcggttttgc caaatttggt gccgtcggat ttggtgacca aaggcagggt
     2161 caagccatat accgtgtctt tgttcaagcg acgggtcaag tctatgccgg cggtgatgtt
     2221 gccccattgg tctgagccac cgatttgcaa tttgcaggca tggcggctgt tcaattgggt
     2281 gaagtcatag gcttgcaaca gactgtaagc gaactcggta aacgaaatac cgacatcatc
     2341 gcggtctaaa cgctgcttca ccgactcttt gttcatcatc gcattcacag agaaatgctt
     2401 accgatgtca cgcaagaaat ccaaacaacc catgccgcca aaccaatcat ggttattggc
     2461 catggttgca gcattgtcgc cgtcaaacga caaaaatggt gtcaattgat tgcgaatttt
     2521 ttctacccaa cctgctacgg tttcaggcgt gttcaggctg cgctcaaccg ctttaaagct
     2581 tgggtcacct atcatgccag tggcaccgcc caccaaagca atcggcttgt gacccgccaa
     2641 ttgaaaacgg cgcaatgcca aaacaggcaa taagtggcca atgtgcaagc tgtctgcggt
     2701 tgggtcaaaa ccacaataca aagaaatctg ttcttgatcc aataattgtt gtaaggcttc
     2761 cgcgtcggtg gtttgggcga tcaaaccgcg ctgttgtaat tcgttgatta atccgctcat
     2821 tgctgctgtc tttacaaaac gatgatagaa aaaagagctt tcaggacgcc tgaaagcctc
     2881 ataacatttg tgcttggcac cattttacag gatagatggg cttggggtct aac
//
LOCUS       DAOMVK010000288         2909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_10107_length_2909_cov_2.827961, whole genome shotgun sequence.
ACCESSION   DAOMVK010000288 DAOMVK010000000
VERSION     DAOMVK010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10107_length_2909_cov_2.827961"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PK856_09060"
     CDS             <1..556
                     /locus_tag="PK856_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02555.1"
                     /translation="LPLKANQTYFKFAQKRVKSLVKQYAKQPDRLMRLLRGTTNMGAA
                     VGLLAGMCVLALPVMGILAAIALPMYADYANKAKFHQAYAELVVLKPAIEEVVQKNIQ
                     VQTDQPSAAGYVDVTGVTSLSDVTTKFDVVGEGFVMGYDLSNHNVQMYLKRDSAGLWH
                     CEMRTTGSNPIRQRDVPTACEIVD"
     gene            complement(706..1557)
                     /locus_tag="PK856_09065"
     CDS             complement(706..1557)
                     /locus_tag="PK856_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02556.1"
                     /translation="MGQFKKYLWAACLMTLLCWGIGSLIHNLYLAAQEDKERMAYEAR
                     LQKLNIPRNENGVRYSIGNIGGQRVQIPPGIVNTWITYDGDPSDFDFDAKKKYKRPPA
                     SYESSIAQFGFKFRISDGALLRLGTQTEVDYQNDSKSEPTPWAYVISRSGNQYPTRER
                     RMVMDVYFKNYINPTSQTPLSYSGQEYGLEVYRFFGGTAPPPQNSWYKEEVYVQRDTA
                     GEITTIIKCTNNPAFAVNSCNQWFYMPKNQMKAFLDVSYPRAYLSEWQKIEQHIITIL
                     NGFALTH"
     gene            complement(1682..2068)
                     /locus_tag="PK856_09070"
     CDS             complement(1682..2068)
                     /locus_tag="PK856_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRL02557.1"
                     /translation="MAEQDYQNGLKVRTEVMGAAFVERAQNNTTELTQPLQDWINEHA
                     WGSTWQREGVLERKTRSLITLAMLTALKCPTELKGHIRGAINNGCTVAEIQETLLHSL
                     PYCGAPAAQEAFRAAQEVLDDMVKQP"
     gene            complement(2185..2559)
                     /locus_tag="PK856_09075"
     CDS             complement(2185..2559)
                     /locus_tag="PK856_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02558.1"
                     /translation="MMLIYMAVSAWRIRKQPQRLPELPLKMLLCLTTIICIALVHMRY
                     HQQARLEANRIAEQIQAYQLQHKAYPADLQAAQAVSTSVSHTHYSLNQNQQPSLFYPA
                     TFWPFEIYAYDFQTQQWQHQPD"
BASE COUNT          687 a          645 c          771 g          806 t
ORIGIN      
        1 gttgccgctc aaagccaacc aaacctattt taaattcgcc caaaaacggg tgaaatcctt
       61 ggtgaagcag tatgcgaagc aacccgatcg gttaatgcgc ttgctgcgcg gcacgacgaa
      121 catgggggct gccgtcggtt tgttggcagg catgtgtgtg ctggctttgc cagtgatggg
      181 catcttggcg gccatcgcct tgcccatgta tgccgactac gcgaacaagg ccaaatttca
      241 ccaagcctat gctgagttgg tcgttctgaa accagccatt gaagaggtag ttcaaaaaaa
      301 tatccaagtc caaaccgacc aaccaagtgc tgctggttat gtggatgtga cgggagtcac
      361 ttcgctcagc gatgtgacca ccaaattcga tgtggtgggc gaaggttttg taatggggta
      421 tgacttgagt aatcacaatg tgcaaatgta tttaaagcgc gacagcgcag gcttatggca
      481 ttgcgaaatg cgcaccactg gctctaaccc catacgccag cgtgatgtgc caactgcatg
      541 cgaaattgtg gattaatacc gaagcagcat gcaaacgtaa caagcggcct gtaaaccttt
      601 acaggccgct tttaatcaca tgattcttct agcggctttt taaaaatgcc tctcggtttt
      661 ggttgagaaa atgtcagacc attgagcttg agttgcacga gggcatcagt gggttaaggc
      721 aaacccgttg agtatggtaa tgatatgctg ctcaattttt tgccattctg ataaatatgc
      781 gcgtggataa ctcacgtcta aaaatgcttt catttgattt ttcggcatat aaaaccattg
      841 attacaagaa ttgacggcaa aggctggatt atttgtacat ttgatgatgg tggtaatttc
      901 accagctgta tcacgctgta catacacttc ttctttgtac caagagttct gtggcggcgg
      961 cgctgttcca ccaaaaaaac ggtaaacctc caatccatac tcctgcccag aataggagag
     1021 cggagtctgt gaggtgggat taatatagtt tttgaagtaa acatccatga ccatccttct
     1081 ttccctcgta gggtactgat ttccacttct ggaaataaca tatgcccaag gagttggttc
     1141 agacttactg tcattttgat aatctacttc tgtttgtgtg cccaatcgca acaatgcccc
     1201 atcgctgatt cgaaatttaa aaccgaactg tgcaatggaa gactcataac ttgcaggcgg
     1261 ccgtttgtat ttctttttag catcaaaatc gaaatcactt ggatcaccat cataagtaat
     1321 ccaagtattc acgattccag gcggaatttg aacccgctga ccgccaatat tgcctatgct
     1381 gtaacggact ccattttcgt ttcgggggat attcagcttt tgcagccgtg cttcataggc
     1441 cattctttct ttgtcttctt gcgcagcaag gtacaagttg tgtatcagcg accctatgcc
     1501 ccagcaaaga agcgtcatca gacatgccgc ccataagtat tttttgaatt gccccatcga
     1561 acattcccct gcttgaatcg cttaagtttg aaacccattc atagctgtat cgaaccatcg
     1621 cacaatgcaa gctgtttagc ttggttaagg ctgactggtc gcttggtttg ggttttcggc
     1681 attaaggctg cttgaccatg tcgtccaaca cctcttgcgc ggcgcggaag gcttcttggg
     1741 cggcgggggc gccgcaataa ggcaggctgt gcagcaaggt ttcttggatt tcggcgacgg
     1801 tgcaaccgtt gttaatcgcg ccgcggatgt ggcctttgag ttcggtgggg cattttaagg
     1861 cggtgagcat ggcgagggtg atgagggaac gggttttgcg ctctaacacg ccttcgcgct
     1921 gccaagtgga gccccaagcg tgttcgttaa tccagtcttg caagggttgg gtcagctcgg
     1981 tggtgttgtt ttgcgcgcgc tcgacaaagg ctgcacccat cacttcggtg cgtactttca
     2041 agccgttttg gtaatcttgt tctgccatgg tctcttcggt ttctgttggt ggatttgtgg
     2101 tgggaattct agcgggtttt gagctgattg tagctgaata cggcgtttga tgcggcctgt
     2161 aagcctttta caggccgctt gtattcaatc gggctgatgt tgccactgtt gggtttggaa
     2221 atcataggca taaatttcaa acggccaaaa tgtggcggga tagaacaagc tgggttgctg
     2281 attttgattg agggagtaat gcgtatggga aacagaggtg gatacggctt gagcggcctg
     2341 taaatctgct ggatacgctt tatgctgtag ctgataagcc tgtatttgtt cggcaatgcg
     2401 gttggcctca aggcgggctt gttggtgata gcgcatgtgt accaaggcga tgcagatgat
     2461 ggtggtgagg caaagcagca ttttcaaggg cagctcaggc aggcgctgcg gctgtttgcg
     2521 tatgcgccat gcactcacgg ccatgtaaat tagcatcatg ggcaccaaaa aatacagcat
     2581 aaaccctgca tgtttttgcc ccaatatcag ggcactgatg gcgcagaata cgatgcaggg
     2641 aatgagggag cggagcatga aaatccttgg gtatcgcggt ggttttgaag atgggaagca
     2701 gctcagattg tgacaaacag gcgcttgtgg agtctttgaa taaacgctgt ttgtaggtct
     2761 tgaatgtgag cattaagcgg cctgtaaacc tcaatatagg cttacaggcc gcttgttttg
     2821 ttacggcatc aattcggcaa cacggctcac tatcgcttcg gccacttggt ctttgcccat
     2881 ggcgggcaat tcggtttcgg cttcgtcat
//
LOCUS       DAOMVK010000289         2899 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_10150_length_2899_cov_4.462729, whole genome shotgun sequence.
ACCESSION   DAOMVK010000289 DAOMVK010000000
VERSION     DAOMVK010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10150_length_2899_cov_4.462729"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1530
                     /locus_tag="PK856_09080"
     CDS             <1..1530
                     /locus_tag="PK856_09080"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HRL02559.1"
                     /translation="QLVAAGDYKAKNIASEDILLIVASTQGEGEPAEEAVSLHKFLHG
                     KKAPQLAQLSYAVLALGDTSYPNYCQAGKDFDAALEKLGATRLAERVDCDLDFQTASE
                     AWRTQVAGVVQQLAAASAAPAAGATPSASSSVVAEQHYTKENPYSATLSVGQKITSDD
                     ANKDVRHIEIDLADSGIHYVAGDALGVWFSNDEVLVAEVLGLAGLSGDEKISLANGQH
                     LSIKEALTFHDELTQNTPQFIKGYAELSQNAALLQLIQDTTALQAAVDCTPIAGILHD
                     YPHAISAEQLHGLLRPLTPRMYSIASAQAEVETEVHLTVGRVAYEHHGQAYTGGASGF
                     LSDRVEEGGEIKVFVEPNKNFRLPENGDTPIIMIGAGTGVAPFRAFIQQRDNNGDAGL
                     NWLVFGNQKFTDDFLYQSEWLQYRKNGLLNKVSTAWSRQGQEKVYVQHKLRENAADVW
                     QWLQNGAHVYVCGDALRMAKDVEQALLDIIAAHGNMSLDDADEYLSELRENKRYQRDV
                     Y"
     gene            complement(1527..2084)
                     /locus_tag="PK856_09085"
     CDS             complement(1527..2084)
                     /locus_tag="PK856_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02560.1"
                     /translation="MWFLNLQTFLTSPWFSLTLFILIFVLVLKIHTLQQQQIKLGSAA
                     SLKQDLLQQTIIQKGENLYQLAAAWLDVSDELQQLNYDYLNARIVPEDYAFQLDHLLD
                     EPGYDVGQIEMLIQVYFPSLLPQFEDIKHSLKRLAEIFDHYDFDPINSNDLARYNQQW
                     EILGLQSTAFKRMLSMQIQMVRPEA"
     gene            2202..>2899
                     /locus_tag="PK856_09090"
     CDS             2202..>2899
                     /locus_tag="PK856_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957994.1"
                     /note="hemoprotein; NADPH dependent; with the alpha
                     subunit (a flavoprotein) catalyzes the reduction of
                     sulfite to sulfide; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit beta"
                     /protein_id="HRL02561.1"
                     /translation="MNTKDPRAKLPDAPLADNERLKGQSNLLRGTIKEDLADELTGGF
                     NGDNFQLIRFHGMYEQDDRDIRAGRVEQKLEPKKNVMLRCRLPGGIIQPQQWLGIDEF
                     ATEHTMYGSIRLTNRQTFQFHGVLKQDIKPMHQWLNKLGLDSIATAGDVNRNVLCTSN
                     PVESALHQEAYEWAKKISEHLLPHTRAYAEIWLDGEKFASTEDGLEQEPVLGTNYLPR
                     KFKTTVVVPPLNDV"
BASE COUNT          767 a          646 c          776 g          710 t
ORIGIN      
        1 caattggtgg cagcaggcga ttacaaagcc aaaaacattg ccagtgaaga cattttgttg
       61 attgtggcct ctacccaagg cgagggtgaa cctgctgaag aagcggtgag cttgcacaaa
      121 ttcttacacg gcaaaaaagc accgcaattg gcgcagttat cgtatgcagt cttggctttg
      181 ggtgatacct cttacccgaa ttactgccaa gcaggcaaag actttgatgc cgccttggaa
      241 aaactgggcg cgacgcgctt ggccgaacgt gtggactgtg atttggactt tcagaccgca
      301 tcggaagctt ggcgtaccca agtggcaggc gtggtgcagc aattggctgc tgccagcgcc
      361 gctcctgcgg ctggtgctac accaagtgca agcagcagcg tggtggccga gcagcattac
      421 acgaaggaaa atccttacag tgcaactttg tcagtaggcc aaaaaatcac ttctgatgat
      481 gcgaacaaag acgtgcgcca catcgaaatt gatttggccg attcgggcat acactatgtc
      541 gcgggcgatg ctttgggtgt gtggttcagc aatgatgagg tattggtggc cgaagtattg
      601 ggcttggcag gtttaagcgg cgatgaaaag atcagcttgg ccaatggcca acacctgagc
      661 atcaaagagg ccttgacctt ccatgatgaa ttaacgcaaa acacgccaca attcatcaaa
      721 ggctatgccg aattgagcca aaatgcggct ttgttgcagt tgatacaaga cacgacagct
      781 ttacaggccg ctgtggattg caccccaatt gcaggcattt tgcacgatta cccccatgcc
      841 atcagtgccg agcaattaca tggcttgttg cgccccttga ccccgcgcat gtattccatc
      901 gcttctgcgc aagccgaagt ggaaaccgaa gtgcatttga ccgtgggccg tgtggcgtat
      961 gaacaccatg gccaagctta cacaggtggt gcatcaggct ttttgtctga ccgcgtggaa
     1021 gagggcggcg aaatcaaagt attcgttgag ccgaataaaa acttccgttt gcctgaaaac
     1081 ggcgacacgc cgatcatcat gatcggtgcg ggcacaggtg tggcaccgtt tcgcgcattt
     1141 atccagcaac gcgataacaa tggcgatgcg ggtttgaact ggttggtgtt tggcaatcag
     1201 aaattcaccg atgacttttt gtaccaatct gagtggttgc aataccgcaa aaacggcttg
     1261 ttgaacaaag tgtctacggc gtggagccgc caaggccaag aaaaggtgta tgtacagcac
     1321 aaattgcgtg aaaacgccgc cgatgtgtgg caatggctgc aaaatggcgc gcatgtgtat
     1381 gtgtgtggcg atgccttgag aatggccaaa gacgtggagc aagccttgct cgacatcatc
     1441 gcagcacatg gcaacatgag cttggacgat gccgatgagt atttgagcga attgcgtgaa
     1501 aacaaacgtt accaacgcga cgtgtattaa gcttcaggcc gcaccatttg aatttgcata
     1561 gacagcatgc gtttgaaggc tgtcgattgc aggcccaaga tttcccattg ttggttgtag
     1621 cgcgccaaat cgttgctgtt gatgggatcg aaatcatagt ggtcgaagat ttcagccaag
     1681 cgtttgagcg agtgtttgat gtcttcaaac tgtggcaata aagatgggaa atacacctgt
     1741 atcagcattt cgatttggcc gacatcgtaa cctggttcat cgagcaagtg gtcgagttga
     1801 aacgcatagt cttcaggcac gatgcgggca ttgaggtagt cgtaattgag ttgttgcaat
     1861 tcatccgaaa cgtctagcca cgccgctgcc aattggtaga ggttctcgcc tttttggatg
     1921 atggtttgtt gcagcaagtc ttgtttgagt gacgcggcac tgcccaattt gatttgttgt
     1981 tgttgcaggg tgtggatttt caagaccaac acgaaaatca gaatgaacag cgtcaaactg
     2041 aaccatggcg aggttaaaaa ggtttgtaaa tttagaaacc acatggcgtt agccccttgt
     2101 ttttatttgt tttaaagatg cggttaagca tcgaatgatt ataattaaat tagagtgagt
     2161 attgtgtact tgttcaaatt taaatttatt tggaatacat tatgaacaca aaagacccac
     2221 gcgccaaatt gcctgatgca cctttggccg acaatgagcg tttaaaaggc caaagcaatc
     2281 tgttgcgcgg aaccatcaaa gaggatttgg ccgacgaatt gaccggtggt tttaatggtg
     2341 acaatttcca actcatccgc tttcacggca tgtatgaaca agacgacaga gacatccgtg
     2401 caggacgtgt ggagcagaag ttagagccca aaaaaaatgt gatgctgcgt tgtcgtttgc
     2461 caggtggcat cattcagcca caacaatggc tgggcataga cgaatttgcc accgagcaca
     2521 ccatgtacgg ctcgatacgc ctgaccaatc gccaaacctt ccaatttcat ggcgtgttaa
     2581 aacaagacat caaacccatg caccaatggt tgaacaaatt gggcttagat tcgattgcca
     2641 ctgcgggcga cgtgaaccgt aatgtgttgt gtacttccaa ccctgtggaa tcggctttgc
     2701 accaagaagc ttacgaatgg gcgaagaaaa tttctgagca tttgctgcca cacacgcgcg
     2761 cctatgccga gatttggttg gatggtgaga aatttgcctc taccgaagat ggtttggagc
     2821 aagagcctgt gttgggcacg aattatttgc cgcgcaaatt caaaaccacg gtggtggtgc
     2881 cgccattgaa cgatgtgga
//
LOCUS       DAOMVK010000290         2883 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_10220_length_2883_cov_2.132603, whole genome shotgun sequence.
ACCESSION   DAOMVK010000290 DAOMVK010000000
VERSION     DAOMVK010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10220_length_2883_cov_2.132603"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..298)
                     /locus_tag="PK856_09095"
     CDS             complement(29..298)
                     /locus_tag="PK856_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02562.1"
                     /translation="MKKIILSSAVAALALTSTLASAAVGVNHNGPQLNPFAQSNAQVQ
                     GNEVTLELVQTGKLNPKEVNPLFKVVSPATTGSTEGQAYSVRVSH"
     gene            complement(502..1197)
                     /locus_tag="PK856_09100"
     CDS             complement(502..1197)
                     /locus_tag="PK856_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - oxidoreductase activity, acting
                     on NAD(P)H, nitrogenous group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HRL02563.1"
                     /translation="MDHHSVEQAITGRHSVRAFTPQAVDKATLLELLNIAARAPSGTN
                     CQPWNVYVATGAARDRIVAEVCALHDAGRSDKSLAEAYSESYPYYPEQWISPYIDRRR
                     ENGWGLYGLLDIQKGEKDKMHAQHQRNFKLFDAPVALFFTVNKAMGIGSKMDIAMMMQ
                     NLMVAAHARGLATCPQAAWNHYHKVVLPIVGAGEDEELVCGMALGYADDSEVVNTFIT
                     PRVKAEDFTRFVE"
     gene            complement(1206..>1560)
                     /locus_tag="PK856_09105"
     CDS             complement(1206..>1560)
                     /locus_tag="PK856_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRL02564.1"
                     /translation="VLLPLKAYVQELIQEMQQVHGDATIVLKQMEQVPAMQSEEGNAL
                     YSLIEGLVDEHKRSKVAYATEGGHFQLADMHAVICGPGSIAQAHKADEYIELTQLARC
                     DRFLQQLLHTQSIKN"
     assembly_gap    1561..1570
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1571..2341)
                     /locus_tag="PK856_09110"
     CDS             complement(<1571..2341)
                     /locus_tag="PK856_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRL02565.1"
                     /translation="MNTVDWLSRLIAFDTTSRHSNLQLIDCVDEYLRGLGLQPWRAWN
                     TDQSKANLFVTLPAHDGNIAGGTVLSGHTDVVPVDGQQWTSDPFQADIRDGKLYGRGA
                     CDMKGFIAACLNMVPQALQTKLREPIHLALSYDEEIGCLGAPVMLNQLQQHGLSPTQC
                     IVGEPTSMQMVVAHKGINVFSCKVHGHSAHSSLTAQGVNAIDYAARIIVFLQGLSKQL
                     QTQHEQDAAFDVPFSTLNVGRIRGGTAVNIIPNECELQF"
     gene            2652..>2883
                     /locus_tag="PK856_09115"
     CDS             2652..>2883
                     /locus_tag="PK856_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02566.1"
                     /translation="MKTHLSGLLLCVLALNVPAETVWSISSSPSQPSQQAHQPNRPYP
                     DRPRPPPRPHPRPPHHHQGPPYYPYYPPHVYPS"
BASE COUNT          656 a          711 c          756 g          750 t
ORIGIN      
        1 ggtttacagg ccgctttggt atggtgtctt agtggcttac gcgcacgcta taggcttggc
       61 cttcagttga acctgtggtg gctgggctca ccactttgaa caatggattc acttctttag
      121 gatttaattt gcctgtttgt accaattcca aagtcacttc attgccttgt acttgggcat
      181 tgctttgggc aaacggattc aattgtgggc cattgtggtt cacgccaacg gcggcacttg
      241 ccaaagtaga agtcaaagcc aatgcggcca cggcgctgct caagatgatt tttttcattg
      301 tgtttacctt tttgattgct gtttttaatt tatgtgacca ttgtatagtt aatcaaaaag
      361 atgagtaagt gcggtttaat taaatgattg ttaatcatta attaatgatg taagcaaaag
      421 cgagtgagtg taaaatatta acaaaaaaca aaaggacgcc ttgaaagcgt cctgtaaata
      481 tgaacaaggg tataaagagg cttattctac aaagcgtgtg aaatcttctg ctttcacccg
      541 tggggtaatg aaggtattca caacctcgct gtcgtcggca tagcccaaag ccatgccaca
      601 caccaattct tcatcctcac ctgcgcccac gataggcaac accactttgt ggtagtgatt
      661 ccaagccgcc tgtgggcaag tggccaagcc gcgcgcatgt gctgccacca tcaggttttg
      721 catcatcatg gcaatgtcca ttttcgagcc tatgcccatg gctttgttga cggtgaaaaa
      781 caaggccaca ggtgcatcaa acaatttgaa attgcgctgg tgttgggcat gcatcttgtc
      841 tttttcgcct ttttggatgt cgagcaagcc gtacaagccc cagccgtttt cgcggcggcg
      901 gtcgatgtaa ggcgaaatcc actgctctgg gtaatacggg taagactcgc tataggcttc
      961 ggccaagctt ttgtcgctgc ggcccgcatc gtgtaaggcg cacacttcgg cgacaatgcg
     1021 atcccgcgct gcgcctgtgg ccacgtacac attccaaggt tggcaattgg tgccagaagg
     1081 ggcacgggcg gcgatgttta acaattccaa cagggtggct ttgtccacgg cttgtggggt
     1141 aaaagcgcgc accgaatggc ggcctgtaat ggcttgctct actgaatggt gatccatgcg
     1201 tgttcctaat tcttaatgga ctgggtatgt aataactgtt gtaaaaagcg gtcgcaacgc
     1261 gcgagctggg ttaattcgat gtattcatcg gctttgtggg cttgagctat gctgccaggg
     1321 ccgcaaatca ccgcgtgcat gtctgccaat tgaaagtggc cgccttcggt ggcgtaagcg
     1381 actttgctgc gcttgtgttc gtctaccaag ccctcaatca agctgtacaa ggcattgcct
     1441 tcttcgcttt gcattgcagg cacttgctcc atttgcttaa gcacaatggt ggcgtcgccg
     1501 tgtacttgct gcatttcctg tatcaactct tgcacatagg ctttgagtgg caacaacacg
     1561 nnnnnnnnnn aaattgcaat tcgcattcgt tggggatgat gttcaccgcc gtgccgccac
     1621 ggatgcgacc gacattcaaa gtgctgaaag gcacgtcaaa cgcggcatct tgctcgtgtt
     1681 gggtttgcaa ttgtttggac aggccttgca aaaacacgat gatgcgggcg gcgtaatcaa
     1741 tcgcgttcac gccttgtgcc gtcagcgatg aatgggcgga atggccatgc actttgcacg
     1801 aaaacacatt gatgcctttg tgcgccacca ccatttgcat gctggtgggc tcgcccacaa
     1861 tgcattgcgt gggcgacaag ccatgttgtt gcaattggtt cagcatcacg ggcgcgccca
     1921 agcagccgat ttcttcgtca taactgagcg ccaagtgtat cggttcgcgc agcttggttt
     1981 gcaaagcctg tggcaccatg ttcaaacaag cggcgatgaa gcctttcatg tcacaggcgc
     2041 cgcggccata caatttgcca tcgcgaatgt cggcttgaaa agggtcgctc gtccattgtt
     2101 gtccatcaac aggcaccaca tcggtgtggc ctgataagac cgtgccgcca gcgatgttgc
     2161 catcgtgagc aggcagggtg acaaacaaat tggctttgct ttggtcggta ttccaagcgc
     2221 gccatggctg caagcccaaa ccgcgcaagt attcgtcaac gcaatcaatc aactgcaagt
     2281 tggaatggcg gctggtggtg tcgaaagcga ttaatcggct taaccaatct acggtattca
     2341 tggcggctgc tcagtgtgaa caagaagcag caaccttacc acaaactgcc caaaaccgtg
     2401 tggcatgcgg cctgtaagat gcttgatgga tatggattgg ctgtgagcag cgattgaatg
     2461 ggtgtgtgtt aaaccatgtt tcacatgaaa cacagcatgg gatacacagg taaatggacg
     2521 gtgatttcag gggtgttttg tggtttacag gccgcttgtt cgcccatttg ggccataaac
     2581 ggcacattta cttcgcctta tcgtgcttat atgtttgtgt gtgatggctt tttcggaaca
     2641 gagaggatga gatgaagacc catttaagtg gcttattgct gtgtgtgctg gctttgaacg
     2701 tgcctgctga aacggtgtgg tcgattagca gctcaccgtc gcagcccagc caacaagcgc
     2761 accaaccgaa tcgaccctat cccgacagac cgcgtccgcc gccccgtccg catccgcgcc
     2821 cacctcatca ccatcaaggg ccgccgtatt atccgtatta cccgccgcat gtttacccga
     2881 gtc
//
LOCUS       DAOMVK010000291         2880 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_10233_length_2880_cov_4.008850, whole genome shotgun sequence.
ACCESSION   DAOMVK010000291 DAOMVK010000000
VERSION     DAOMVK010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10233_length_2880_cov_4.008850"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PK856_09120"
     CDS             complement(<1..586)
                     /locus_tag="PK856_09120"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRL02567.1"
                     /translation="MDSIPLWQAEAARLALAKMAPDVVGEVEPVLPRLFQALADGDSC
                     IALAKHEQARLHGAKPIVGGPQQASTPLIQFADKLTLGRVWQLEAEIASHLHRLSQPV
                     NPEQDLSGLLQTLFDAQSSSEQQQAAALACVNQLAIITGGPGTGKTTTVAKLLCLLSF
                     GMAVPPRIALAAPTGKAAARMTESLRFSLQQLRHN"
     gene            complement(604..1308)
                     /gene="lolD"
                     /locus_tag="PK856_09125"
     CDS             complement(604..1308)
                     /gene="lolD"
                     /locus_tag="PK856_09125"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HRL02568.1"
                     /translation="MSNDLVLACYNVSRTYQDGKLKVDVIQNLDLEVKPGESVSIVGA
                     SGSGKSTLLHIMGGLDTPSSGEVRLMGQPLKDVSQKDLGLLRNQHLGFVYQFHHLLPE
                     FTALENVKMPLLIGKVNKAEAEKRAIEMLEKVGLGSRIDHRPSELSGGERQRAAIARA
                     LVTKPKCLLADEPTGNLDRKNAQVILDLMLDLQRELGTALVVVTHDEELAARFGRMLT
                     MREGHLMPYSLATEQL"
     gene            complement(1301..2551)
                     /locus_tag="PK856_09130"
     CDS             complement(1301..2551)
                     /locus_tag="PK856_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HRL02569.1"
                     /translation="MAQLETWIGLRYLRSKKRNGFVSFISVMSVAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLSVAPHMEIGFIDEGGVRPWQDLQKIVAGNKEVLASAPYVADQA
                     LLANSGEMRGVQLRGIDPAQEGRVVDYANKVEHGKLADLREGEFNIILGKDLAMLLGV
                     QVGEKVTVITPEGNVTPAGVTPRLKQFNVVGVVQTKMFEVDSSLAMINLKDAQVLYRM
                     GDAVSGVRLKLANPQEAPALASTIIPKAEQTSVWARDWTYQNRSYFEAVELEKKMMTI
                     ILSCIVIVAAFNLVSSLVMAVNEKQADIAILRTLGMSPASIMKIFMVQGAVAGFFGTL
                     FGVVAGVILGLNIGKIVHWYEQLTGQRLINAQTYFIDYLPSDVRLSDVLIIAAISLLM
                     AFLATLYPSWAAARTQPAEALRYE"
     gene            2696..>2880
                     /locus_tag="PK856_09135"
     CDS             2696..>2880
                     /locus_tag="PK856_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HRL02570.1"
                     /translation="MNKEYMQQAISEAQAGIHAGHGGPFGCVIVKDGKVIGSGHNEVL
                     KQRDPTCHGEVVAIRDA"
BASE COUNT          704 a          847 c          689 g          640 t
ORIGIN      
        1 tgttgtggcg caattgctgc aaagaaaagc gcagcgattc ggtcatgcgc gcggcagctt
       61 tgcctgtggg cgctgccaaa gcaatgcgcg gcggcacggc catgccaaag cttaacaaac
      121 acaacaattt tgccacggtg gtggttttgc ctgtgcctgg gccgccggtg atgatggcca
      181 attggttgac acaggcgagc gctgccgctt gttgttgctc actgctggac tgggcatcaa
      241 acaaggtttg caacaggcca gacaagtctt gctcagggtt tacaggctga cttaaacggt
      301 gcaaatggct ggcaatctcg gcttccaatt gccacacccg ccccaaagtg agcttgtcgg
      361 caaactgtat caagggcgta gacgcttgct gtggcccacc cacgatgggt ttggccccat
      421 gcaaacgcgc ttgttcgtgt ttggccaagg caatgcaact gtcgccatcg gccaaggctt
      481 gaaacaagcg tggcaacact ggctcaacct cgcccaccac atcgggggcc atctttgcca
      541 aagccaaacg ggcggcttct gcctgccaca gtggaatgct gtccataagc gtcctgtaaa
      601 agattacaac tgttcggtgg ccaagctgta tggcatcaaa tggccttcgc gcatggtgag
      661 catgcgcccg aagcgggcag ccaattcttc gtcgtgtgtg accaccacca aggctgtgcc
      721 caattcgcgt tgcaaatcca gcatcaaatc caaaatcact tgcgcatttt tgcggtcgag
      781 attgcctgtt ggctcatcgg ccagcaagca tttgggcttg gtcaccaagg cgcgggcgat
      841 ggcggcacgt tggcgctcac cacccgacaa ttcggatgga cggtggtcta tgcgactgcc
      901 caaaccgact ttttccaaca tttcgatggc gcgcttttcc gcttcggcct tgttcacctt
      961 gccaatgagc aatggcattt tcacgttttc caaggcggtg aattcgggca ataaatggtg
     1021 aaattggtac acaaatccca aatgctgatt gcgcaacaaa cccaagtctt tttggctcac
     1081 gtctttcaaa ggctggccca tcaagcgcac ctcgccgctg ctcggtgtgt ccaagccgcc
     1141 catgatgtgc agcaaagtcg atttgccact gcccgatgcg cccacaatgc tcacgctctc
     1201 gcctggcttc acttccaaat ccaaattttg aatcacatcc actttgagct tgccgtcttg
     1261 ataggtgcgc gacacattgt agcaggccaa taccaaatca ttactcatag cgcaaagcct
     1321 ccgcaggttg ggtgcgggcg gctgcccagc ttgggtacaa agtggccaaa aaagccatca
     1381 acaatgaaat cgcggcaatg atcaacacat cgctcaagcg cacatcgctt ggcaaataat
     1441 caatgaaata cgtttgcgca ttgatcaggc gttgtccggt taattgctcg taccaatgca
     1501 caattttgcc gatgttcagg ccgagtatca ccccagccac cacaccaaac aaagtgccga
     1561 agaagccagc caccgcgcct tgcaccatga agattttcat gatgctcgca ggcgacatgc
     1621 ccaaggtgcg caagatggcg atgtcggctt gcttctcgtt caccgccatc accaaagacg
     1681 acaccaaatt aaacgcggcc acaatcacaa tacagctcaa aatgatggtc atcattttct
     1741 tttccaattc cacggcttca aaatagctgc ggttttggta agtccaatcg cgtgcccaca
     1801 cactggtttg ctcggctttg ggtatgatgg tgctagccaa agctggcgct tcttgcggat
     1861 tggccagttt caagcgcaca ccactcaccg catcgcccat gcgatacaac acttgcgcgt
     1921 ctttcaaatt gatcatggcc aagctggaat ccacttcaaa catcttggtt tgcaccacgc
     1981 ccaccacatt gaattgcttc aagcgcgggg tcacaccagc aggcgtcaca ttgccctcag
     2041 gggtgatcac ggtgactttt tcacccactt gcaccccaag caacatcgcc aaatctttgc
     2101 ccagaatgat gttgaactca ccctcgcgca aatcggccaa cttgccgtgt tcgactttgt
     2161 tggcgtaatc caccaccctg ccctcttgag cagggtctat gccgcgcaat tgcacgccgc
     2221 gcatctcgcc cgaattggcg agcaaggctt ggtcggccac atacggcgca gaagccaaaa
     2281 cctctttatt gcctgcaacg attttttgca aatcctgcca cggtcgcaca ccaccttcat
     2341 ctatgaagcc gatttccatg tgcggcgcca ccgacaacag ctgaccacgt atctcttttt
     2401 gaaacccatt catcaccgac aacaccacaa tcagcgcggt cacccccaaa gcaatgcccg
     2461 ccaccgacat caccgaaata aacgacacaa aaccattgcg ctttttcgag cgcaaatagc
     2521 gaagtcctat ccacgtttcc aactgagcca ttgatagcct aaaccgttga cataaagaat
     2581 gcgattgtag cgagtttggc agcgtcctga aagcaagcct ctgcctgttt acttgttcta
     2641 acttggcaaa tttggcatca tccagcgtca aaacacagaa ttccaaggca gtcccatgaa
     2701 caaagaatac atgcaacaag ccattagcga agcccaagca ggcatacacg caggacatgg
     2761 cggcccgttt ggttgcgtca tcgtcaaaga tggcaaagtg ataggctcag gccacaatga
     2821 ggtattaaag caacgcgacc ccacttgcca cggcgaagtg gtggccatac gcgatgcctg
//
LOCUS       DAOMVK010000292         2862 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_10320_length_2862_cov_3.105807, whole genome shotgun sequence.
ACCESSION   DAOMVK010000292 DAOMVK010000000
VERSION     DAOMVK010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10320_length_2862_cov_3.105807"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1304
                     /locus_tag="PK856_09140"
     CDS             <1..1304
                     /locus_tag="PK856_09140"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRL02571.1"
                     /translation="QGVGMFYQYVIVGGGAGGVELAARLGRQFGREQGRDKVLLIDRS
                     VFHIWKPTLHEVAVGTLNPQQEGLSYSILARRNNFSFTVGNLVGLDAEQKQLTLASVY
                     TDDQDHDLIIPERTISFERCILAMGSGSNFFNTKGAAEHAYVLENAQDAQRFQRHLLD
                     LFAKAAYSPQKSLNVVIIGGGATGVELAAEMTEAYHEILQTFGSPQRFDIKITLIESS
                     PRILAGLPETVSTQATSALQQKGIQVLTSTRAEEVGRDFVQTDHTRIEADVVVWAAGI
                     KAADINPSFGLPVNRINQFEVNAQLQTPADAVYAMGDCAACKWEDDKLLPARAQVAHQ
                     QATYLTRLLKAHDAGQGFNDAFVYKDSGSLVSLGDNKGVGNLMGSLSGKSFFIEGLVA
                     KWMYMSLHLMHHRAILGTPKTILLAFARLAQRRVSGRLKLH"
     gene            1331..2107
                     /locus_tag="PK856_09145"
     CDS             1331..2107
                     /locus_tag="PK856_09145"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HRL02572.1"
                     /translation="MNAPAEAKFTEETVLWVKRHTDKLMTFAITRPEGYRFSAGQFSR
                     LGFRDGEGYLWRAYSIVSAEYDDTLEYFVVLIDGGPISAKFALLQEQDTLLLDKTVTG
                     FLLPERFADGRDLVMLCTGSGIAPFLSQLQQADVYQRFERIALVHSVSHQNELIFNQR
                     VQDLAEHPLVEDYQGKLSFQAVVTREATDGALNARIPELLKDGSLAKALGWEFAPEHT
                     RFLICGNPAMVHDTFQALLSMGFAMHRNRIPGQIIMENGF"
     gene            2139..2717
                     /locus_tag="PK856_09150"
     CDS             2139..2717
                     /locus_tag="PK856_09150"
                     /inference="COORDINATES: protein motif:HMM:NF019204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HRL02573.1"
                     /translation="MNTAKLYCITMGATVPGRLIEQHDVMFAAAADISDLIPSLQAAW
                     PEGASVHVDAYREVTVVGSHHISLTQDPVSADASGLKLFFFNLGGYVEGDMEEYHHKA
                     LVIAASQKEAIASIKKCEFYQNRGYAGAESHIDDKMNVDIDDVHIVDDLISHAGWHLH
                     ISPASQTATEDVWQVGYLSLSKLAKRAASLEA"
     gene            2769..>2862
                     /locus_tag="PK856_09155"
     CDS             2769..>2862
                     /locus_tag="PK856_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HRL02574.1"
                     /translation="MSIRTRFAPSPTGYLHIGGVRTALFSWAFAK"
BASE COUNT          716 a          753 c          695 g          698 t
ORIGIN      
        1 atcaaggagt tggcatgttt tatcaatatg taattgtcgg cggtggcgct ggcggtgtgg
       61 aattggcagc ccgtttgggc cgacaatttg gacgtgagca aggacgcgac aaagtcttgc
      121 tcatagaccg ctcggtattt cacatttgga agcccacttt gcacgaagtg gccgtaggca
      181 ccttaaaccc acaacaagaa ggcttgtctt acagcatctt ggcacgccgc aacaacttca
      241 gcttcaccgt gggcaatttg gtgggcttgg atgctgagca aaagcaattg accttggctt
      301 cggtctacac cgatgaccaa gaccatgatt tgattatccc tgaacgcacc atcagctttg
      361 agcgctgcat cttggccatg ggaagcggct ccaatttctt taataccaaa ggtgcagccg
      421 aacacgctta tgtcttggaa aatgcccaag atgcccagcg ctttcaacgc catttgctcg
      481 acttgtttgc aaaagcggct tactcgcccc aaaaaagcct gaatgtggtc atcattggtg
      541 gcggcgcgac tggggtggaa ttggcggcag aaatgactga ggcctatcat gaaatcttgc
      601 aaacgtttgg gagcccacaa cgctttgaca tcaagattac cttaatcgaa tcttcacccc
      661 gcatcttggc aggtctacct gaaaccgtgt ccacccaagc caccagcgcc ttgcaacaaa
      721 aaggcataca ggtattgacc agtacccgtg ctgaagaagt gggccgtgac tttgtccaaa
      781 cagaccacac acgcatcgaa gccgatgtgg tggtgtgggc ggccggtatc aaagccgccg
      841 acatcaaccc atcatttggt ttgcctgtta accgcatcaa ccaatttgaa gtcaatgccc
      901 aattacaaac ccctgccgat gcggtctatg ccatgggcga ttgtgcggcc tgtaaatggg
      961 aagatgacaa attactgcca gcacgcgcgc aagtcgccca ccaacaagcc acttatttga
     1021 cgcgcttatt gaaagcccat gatgcgggac aaggattcaa tgatgccttt gtctacaaag
     1081 attctggctc attggtgtct ttgggcgaca acaaaggcgt gggcaacctc atgggcagct
     1141 tgagtggcaa aagttttttc attgaaggct tggtggccaa gtggatgtac atgtctttgc
     1201 acttgatgca ccaccgcgcc atcttaggca cgcccaaaac catcttattg gctttcgcac
     1261 gcttggctca acgccgcgtt tcaggccgtt taaaactaca ttgatattga ttgaatcaca
     1321 gagaattttt atgaacgcac cagccgaagc aaaatttacc gaagaaaccg tcttatgggt
     1381 caaacgccat accgacaaat tgatgacgtt tgccatcacc cgccccgaag gctaccgctt
     1441 cagcgcaggc caattctcgc gcttgggctt tcgcgatggt gaaggctatc tttggcgcgc
     1501 ttactccatc gtttcagccg aatacgatga caccttagaa tactttgtgg tcttgataga
     1561 cggtggccct atttcggcca aattcgcctt attgcaagaa caagacaccc tcttgctcga
     1621 caaaaccgtc acaggctttt tgcttccaga gcgttttgcc gatggccgtg atttggtgat
     1681 gttgtgcacg ggttcaggca tagccccatt cttatcgcag ttgcaacagg ccgatgtata
     1741 ccaacgtttt gaacgcatcg ccttggtgca ctcggtgtcc catcaaaacg aattgatttt
     1801 caaccaacgg gtgcaagatt tagctgaaca cccattggta gaagactacc aaggcaagct
     1861 gtctttccaa gccgtggtca cccgtgaagc cactgatggc gctctcaatg cacgcatacc
     1921 cgagctgctc aaagatggca gcttagccaa ggctctgggc tgggaatttg cccccgaaca
     1981 cacccgcttt ttgatttgtg gcaaccctgc catggtgcac gacaccttcc aagcgctgtt
     2041 aagcatgggg tttgccatgc accgcaaccg cattccaggt caaatcatca tggaaaacgg
     2101 cttctaaaac ccaatcatac tcactacaca ggccacacat gaacactgcc aaattgtatt
     2161 gcatcaccat gggcgccacc gtccctgggc gcttgataga acaacatgat gtgatgtttg
     2221 ccgctgctgc cgacatcagc gatttgatac ccagcttaca ggccgcttgg cctgagggtg
     2281 catcggtgca tgtggatgcc taccgtgaag tgactgtggt gggttctcat cacatcagtc
     2341 tgactcaaga ccctgtctca gcagatgcaa gcggcctgaa actgtttttc tttaatttgg
     2401 gcggctatgt cgaaggcgac atggaagaat atcaccacaa agccttggta atagctgcct
     2461 ctcaaaaaga ggccatcgcc agcatcaaga aatgtgagtt ttaccaaaac cgcggctatg
     2521 cgggcgccga atcacacatt gatgacaaaa tgaatgtcga catagacgat gtccacattg
     2581 tggatgattt aatcagccac gcaggttggc atttgcacat ctctcccgcc tcccaaacag
     2641 ccacagaaga tgtgtggcaa gtaggttatt tatcgttaag taagctggcc aaacgtgccg
     2701 cctcacttga ggcttgaagc gtcagtgcag tacaatacgc gattatttga aatttgaaag
     2761 aagcagacat gtccatccgc acccgttttg cgcccagccc tactggttat ttacacattg
     2821 gtggtgtgcg cacggctttg ttttcttggg cttttgccaa aa
//
LOCUS       DAOMVK010000293         2842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_10408_length_2842_cov_2.980266, whole genome shotgun sequence.
ACCESSION   DAOMVK010000293 DAOMVK010000000
VERSION     DAOMVK010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10408_length_2842_cov_2.980266"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PK856_09160"
     CDS             complement(<1..235)
                     /locus_tag="PK856_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRL02575.1"
                     /translation="MDHNNNIAQLKDSGLKVTGPRLKILDLFEQRPEAHLSAEDVYRL
                     LLEDESDIGVATIYRVLTQFEQAEILIRHHFETG"
     gene            371..754
                     /gene="bamE"
                     /locus_tag="PK856_09165"
     CDS             371..754
                     /gene="bamE"
                     /locus_tag="PK856_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003780297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HRL02576.1"
                     /translation="MNKFIVLTLAVAAVGLSACSSERVSNFPSYKLKVEQGNELDANK
                     VQQLQAGMTREQVRLLLGTPSIRSAFHANRWDYQFLISRNGVVKENHNLTVYFDGDLL
                     SRYEGSALQAAGHSQAAEPQAAPAE"
     gene            816..1628
                     /gene="dapB"
                     /locus_tag="PK856_09170"
     CDS             816..1628
                     /gene="dapB"
                     /locus_tag="PK856_09170"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRL02577.1"
                     /translation="MSELLNVAIVGANGRMGRALVQAIHDHPQCRLSGALEHDQSPAL
                     GMDAGFTLGLETGVEITHDAVKVLSQSDVLIDFSRPESTLNYLTACEKLGINMIIGTT
                     GFDDAGKARIAEAAKKIGIVFAANYSVGVNLTFALIHKAAQVLNDGFDIEVIEAHHRH
                     KVDAPSGTALRMGEVLADAIGKDLKDVGVFAREGITGPREAGTIGFATVRGGDIVGDH
                     TVMFAADGERVEVTHKASSRATFANGAVRAAAWLRNQPVALYDMQDVLNLRW"
     gene            1869..>2842
                     /locus_tag="PK856_09175"
     CDS             1869..>2842
                     /locus_tag="PK856_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ionic transporter y4hA"
                     /protein_id="HRL02578.1"
                     /translation="MAANSSKHNPLWTVIVPVISMLILGIYALNPHFFGIVTLLTLGL
                     VGSVIAAVHHAEVIAHRVGEPFGTLVLAMAVTIIEVALIVSMMMSGGEATMGLARDTI
                     FAAVMIILNGIIGASLLLGARKHLVQDFSLEGVTAALGTMTVIVTFALILPSFSTTAP
                     GPVYTSNQLMFVAIMTLVLFAAFTFFQTVRHRDYFLPVVQETDLGNIDSHANPPNNKD
                     VGISFVCLLLSLVAVVLSAKGISPSLEKLLSVVGAPAATLGIIIAAIVLMPEFGAALR
                     AARANRLQTSLNLAIGSAIASIGLTIPVVAIVAIVMDWPLELGLDAKS"
BASE COUNT          650 a          738 c          718 g          736 t
ORIGIN      
        1 tacccgtttc aaaatggtgg cgaattaaga tttccgcttg ttcaaattgg gttaaaacac
       61 ggtatatggt ggccacaccg atgtcgcttt cgtcctccag caacaagcgg tacacatctt
      121 cagcactcag gtgcgcttct gggcgctgtt caaacaagtc caaaattttc aaacgcgggc
      181 cggtgacttt caggccgctg tcttttaatt gggcaatatt attgttgtga tccatgatga
      241 ttcccacgaa ttgacataag tcgctataat acccactttc tgagtgatag accaccttac
      301 tgacgcccct acaatgggct tcatggctgg ctcaccgaaa ccgcacatcc cgttttgaat
      361 cgaggtaacc gtgaataaat ttatcgtcct gactttagcc gttgctgccg tgggtttaag
      421 tgcctgttct tcagaacgcg tttccaattt tccttcctac aaactcaagg tagaacaagg
      481 caatgagttg gatgccaaca aagtgcaaca gttacaggcc ggcatgacac gtgaacaagt
      541 gcgcttgttg ttgggcacgc ccagcatacg ctctgccttc catgccaacc gctgggatta
      601 ccaattcctg atttcacgca atggcgtggt caaagaaaac cacaatttga ccgtgtattt
      661 tgatggcgac ttgctcagcc gttatgaagg ttcagcctta caggccgctg gtcacagcca
      721 agctgccgag ccgcaagcgg ccccagccga ataatactga cggtggtctt gtaccaccgt
      781 tatttgtttg acctgtccaa aataagtaga gaaacatgtc tgaattattg aatgtagcca
      841 tagtgggcgc caacggtcgc atgggccgag ccttggtaca agccatacac gaccatcccc
      901 aatgccgttt gtctggcgct ttagaacacg accaatcgcc agccttgggc atggacgcgg
      961 gttttacctt gggcttggaa acaggcgtgg aaatcaccca cgatgcggtg aaggtattga
     1021 gccaaagcga tgtattgatt gatttcagcc gtcctgaatc cactttaaac tacctgacag
     1081 cgtgcgaaaa actcggcatc aacatgatca tcggcaccac aggttttgat gatgcgggca
     1141 aggcgcgcat tgccgaagcc gccaagaaaa ttggcatcgt gttcgccgcc aattacagcg
     1201 tgggcgtgaa tttgacgttt gccttaatcc acaaagccgc gcaagtgttg aacgatggct
     1261 ttgacattga agtgatagaa gcccaccacc gccacaaagt tgatgcccca tcgggcacgg
     1321 cgctgcgcat gggcgaagtg ttggccgatg cgataggcaa agatttgaaa gacgtgggcg
     1381 tgtttgcgcg cgaaggcatt acaggtccac gcgaagcggg caccataggc tttgccactg
     1441 tacgtggtgg cgacatagtc ggcgaccaca cggtgatgtt tgccgccgac ggcgaacgcg
     1501 tcgaagtgac ccacaaagcc tctagccgcg ccacgtttgc caacggtgcg gtacgcgcgg
     1561 cagcatggtt gcgcaaccaa cctgtggcct tgtacgacat gcaagacgtg ttgaatttgc
     1621 gttggtaaac caagccaatt caaacgcatt aagcggcctg taaacagttt acaggccgct
     1681 ttttattggc aaaagcggaa tgcttattct tgacaccaac acaccaaagc ctttatttta
     1741 ataagcgatt ttgagtttac aaggtttgtg cagcgtgttt tcgatgcaaa catcttgcaa
     1801 aactttctca cccacttcag tacctcgcta cctaaaacca ttacgccaac ccctattttg
     1861 agaccaagat ggcagccaat tcttctaaac acaatcctct gtggacggtc atcgtccccg
     1921 tcatttccat gctcattctt ggcatttatg cacttaatcc ccattttttt ggcatcgtga
     1981 ctttgttaac cttgggtctg gtcggcagcg tgattgcagc cgttcaccac gccgaagtca
     2041 tcgcccaccg cgttggtgaa ccttttggca ctttggtatt agccatggct gtgaccatta
     2101 ttgaagtggc gttgattgtg tcgatgatga tgtctggcgg cgaagccacc atgggtttgg
     2161 cgcgagacac catttttgca gcggtgatga ttattttgaa cggcatcata ggcgcgagtt
     2221 tgctgctggg tgcgcgcaaa cacttggtgc aagacttcag cttggaaggc gtgaccgcgg
     2281 ctctgggcac gatgacggta atcgtcacat ttgccttgat tttgcccagc ttctccacca
     2341 ccgctcctgg ccctgtctac accagcaatc aactgatgtt tgttgccatc atgacgcttg
     2401 tgttgtttgc cgcgttcacg tttttccaaa ccgtgcgcca ccgcgattat ttcttgcctg
     2461 tggtacaaga aaccgatttg gggaacatcg attcccacgc caacccacct aataacaaag
     2521 acgtgggcat cagttttgtg tgtttgttgc tgtcattggt ggcggtggtg ttgagcgcca
     2581 aaggcatttc accttcgctt gaaaaactgc tttccgtggt gggtgcccct gcggccacac
     2641 tgggtatcat cattgccgcc atcgtgttga tgccagagtt tggtgctgct ttgcgtgcgg
     2701 cgcgtgccaa ccgtttgcaa accagtttga atttggccat aggctcggcc attgccagca
     2761 taggcttgac cattcccgtg gtggccatcg tcgccattgt gatggattgg ccactggaat
     2821 tgggcttgga tgccaaatcc at
//
LOCUS       DAOMVK010000294         2839 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_10419_length_2839_cov_2.427083, whole genome shotgun sequence.
ACCESSION   DAOMVK010000294 DAOMVK010000000
VERSION     DAOMVK010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10419_length_2839_cov_2.427083"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="PK856_09180"
     CDS             <1..586
                     /locus_tag="PK856_09180"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003789165.1"
                     /note="converts 2-oxoglutarate to glutamate; in
                     Escherichia coli this enzyme plays a role in glutamate
                     synthesis when the cell is under energy restriction; uses
                     NADPH; forms a homohexamer; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HRL02579.1"
                     /translation="GEKVVTLSDSNGMIYVKDGISEAMLLDIMDLKNVKRGRLEDLAN
                     VAGIEYIAGKRPWTVPVDIAMPCATQNELNGEDAALLIHNGVKCVAEGANMPSTLDAV
                     DAFVAAQVLYAPGKASNAGGVATSGLEMSQNGIRLNWTHGEVDERLHGIMKDIHGNCV
                     RYGKQEDGYINYVNGANIAGFVKVADAMLAQGVY"
     gene            741..1280
                     /locus_tag="PK856_09185"
     CDS             741..1280
                     /locus_tag="PK856_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HRL02580.1"
                     /translation="MFDHKIIITGAVGVGKSTAIRTYSTSAVVDTDVRATDETSQIKK
                     DTTVAMDYGTITLDAETKVHLYGTPGQERFDFMWEILRSGSRGLIVLVNAQSTDPGQE
                     LRFYLHAFADVVNKVPVVVGLNQTDKNEHSSAMVAELQQVVEEVGVSADVLVVDVRKR
                     KDVNKLVKRILFSDKLFAV"
     gene            complement(1348..2670)
                     /locus_tag="PK856_09190"
     CDS             complement(1348..2670)
                     /locus_tag="PK856_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HRL02581.1"
                     /translation="MKTVTSTRFRLAAAASLAVLLSACPTTKPIGGGTPSGGPTSALP
                     PTPPGQVYGTPGQAVRYTSASFSQVPEWSGQSFKNSLISFQRGCSKLTGKVQWQAVCN
                     QAKNTPQTDTTARLFFENLFTPWQLSDNGNNSGTVTGYYEPVLHGDTQQTSQARFPIY
                     GIPTDFVSVPLSDENRLRKGTVRVSVTGANQGNINGSGTYTANLNAFPITERSRSIKG
                     RVVGNQFVPYYTRAEINAGALNGKAPILAYADDAVELFFLHIQGSGRLQTPSGQFVRL
                     GYADKNEYPFKSVATYMAGKGYLPLAQTNMQGIKAWMRQNPSKLSEVLGQNPSYVFFR
                     TTTGAADEGPIGALGAPLTGEYSAAIDKHHVELGAPIFVATTHPLNNKPHNRLIVAQD
                     TGSAIKGGVRVDYYWGYGDAAGEVAGKMKHPGQVWMLLPNGYTPRYQP"
BASE COUNT          698 a          625 c          796 g          720 t
ORIGIN      
        1 gggtgaaaaa gtcgtgacat taagtgattc caatggcatg atttacgtga aggatggtat
       61 ttctgaggcg atgttgctcg acatcatgga cttgaaaaac gtcaaacgtg gccgcttgga
      121 agatttggcc aatgtggctg gcattgagta cattgcgggc aaacgcccat ggaccgtgcc
      181 tgtcgacatc gccatgcctt gcgccactca aaatgagttg aatggcgaag acgcggcttt
      241 gttgatacac aatggtgtga aatgtgtggc cgaaggcgca aacatgccgt ctactttgga
      301 cgctgtggac gcatttgtgg ccgcgcaagt gttgtatgca ccgggcaaag catcgaatgc
      361 cggtggtgtg gccacctctg gtttggaaat gtcgcaaaat ggcatccgtt tgaattggac
      421 gcatggtgaa gtagacgaac gtttgcatgg catcatgaaa gacattcacg gcaattgtgt
      481 gcgctatggt aaacaagaag atggttacat caactatgtg aatggcgcga acattgcggg
      541 ttttgtaaaa gttgctgatg caatgctggc tcaaggtgtg tattgatgtg ttcattacaa
      601 cattaatata aaaactttct cataagccca gcaccgcgct gggttttttt atgtcttgac
      661 gtataatgct gaaaatacga aactgaaaaa taactctaat aatttgtgtt gtcattcaat
      721 accgctcatg gggaaacacg atgttcgacc ataaaatcat cataacaggt gctgttgggg
      781 ttggaaaaag caccgccata cgcacttaca gcacctctgc tgtggtagac accgatgtgc
      841 gtgccaccga tgaaaccagt caaatcaaaa aagacaccac tgtggccatg gattacggca
      901 ccattacctt ggatgcggaa accaaagtgc atttgtatgg cacgccaggg caagagcgtt
      961 tcgattttat gtgggaaatt ttgcgcagtg gcagccgtgg tttgattgta ttggtcaatg
     1021 cccagtcgac agaccctggc caagaattgc gcttttacct gcacgcgttt gctgatgtcg
     1081 tgaacaaagt gccggtggtg gtgggattga atcaaacgga taaaaacgag cacagcagtg
     1141 ccatggtggc agagttgcag caggtggtgg aagaggtggg cgtgagcgcc gatgtgttgg
     1201 tggtggatgt gcgcaaacgc aaagatgtga ataaattggt gaaacgcatt ttgttttcag
     1261 acaaattgtt tgcggtttaa aagaatcaaa caaaacaaat aagcggcttg gtttacaggc
     1321 cgcttatttg tattttaaaa gcccgattta aggctggtaa cgtggcgtat agccattggg
     1381 caacaacatc catacctgac ctgggtgctt catttttcca gcaacttcgc cggccgcatc
     1441 gccatagccc cagtagtaat cgacacgcac gccgcctttg atggcagagc ccgtgtcttg
     1501 tgccacgatg aggcggttat gcggcttgtt attgagcgga tgggtggtgg ccacaaaaat
     1561 cggcgcgccc aattcgacat ggtgtttgtc gatggcggct gaatactcgc ctgtgagcgg
     1621 tgcacccaaa gcgccaattg ggccttcatc ggccgcgcct gtggtggtgc ggaagaacac
     1681 atagcttggg ttttgcccca agacttcaga caatttgctt gggttttggc gcatccatgc
     1741 tttgatgcct tgcatattgg tttgggccaa aggcaaatag cctttgcctg ccatgtaagt
     1801 ggccaccgat ttgaatgggt attcgttttt gtcggcatag cccaagcgca caaattggcc
     1861 tgatggcgtt tgcaagcggc ctgaaccttg aatgtgcaag aaaaacagct ctaccgcatc
     1921 atcggcatag gccagaatcg gggctttgcc gttgagcgcg cctgcgttga tttcagcacg
     1981 ggtgtaatac ggcacaaatt gattgcccac cacgcggcct ttgatgctgc ggctgcgttc
     2041 ggtaatcgga aacgcattca aattggcggt gtaagtaccg ctgccattga tgttgccttg
     2101 attggctcct gtgacagaaa cgcggacagt acctttgcgc agacggtttt catccgataa
     2161 aggcacgctg acgaaatcgg tgggtatgcc ataaatgggg aagcgtgctt gagaagtctg
     2221 ttgggtgtcg ccatgtaaaa caggctcgta atagccagtg accgtgccgc tgttattgcc
     2281 attgtcactg agttgccaag gcgtgaacag gttttcaaaa aacagccgcg ctgtagtgtc
     2341 ggtttgcggc gtgtttttgg cttgattgca cacagcttgc cactgcactt taccggtgag
     2401 tttgctacag ccgcgttgga atgaaatcaa actgttttta aacgattgac ctgaccattc
     2461 gggcacttgg ctgaatgagg ctgaggtata gcgcacggct tggcctggtg tgccatacac
     2521 ttggcctggt ggcgtgggcg gcagggcaga agtaggcccg cccgagggtg tgccacctcc
     2581 tattggtttg gtggtggggc aggcactgag taacactgcc aaagaagcgg cagcagcaag
     2641 gcggaatcgg gtcgaggtca ctgttttcat aggctttata tgacaaaagc agccaaggct
     2701 gcctttgtat tcatcgggaa tgaaatcaaa cattatttga atggcattga tactttcaca
     2761 ccaacacccg caccatcata agtcggtaca tcggcggcgt agttgtacca cacggtagaa
     2821 tccacgttca aaccatttt
//
LOCUS       DAOMVK010000295         2833 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_10451_length_2833_cov_2.537797, whole genome shotgun sequence.
ACCESSION   DAOMVK010000295 DAOMVK010000000
VERSION     DAOMVK010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10451_length_2833_cov_2.537797"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            263..904
                     /locus_tag="PK856_09195"
     CDS             263..904
                     /locus_tag="PK856_09195"
                     /inference="COORDINATES: protein motif:HMM:NF007980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HRL02582.1"
                     /translation="MTDAFFDWSLWRRSVGADLSQVQYPNLKAQLPYLQQMDITEFKP
                     AAVMVLIEPGSVAQVVLTVRSLQLHQHGGQVSFAGGKKDPEDGNLIETALRETHEELG
                     IPPNNIEVLGQMPSLPTVSAYNVSPVIGLLKPHTNIVPNVGEVAKVFLVPLAQLMQPK
                     RYLHYPIMRGNQVFQTVKFAHTENIWGATATILLALAEHYQAYARDTRYGFDE"
     gene            888..1580
                     /locus_tag="PK856_09200"
     CDS             888..1580
                     /locus_tag="PK856_09200"
                     /inference="COORDINATES: protein motif:HMM:NF014007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HRL02583.1"
                     /translation="MVLMNEDVPLWLAYGSIALLLLMVGYVCQDKALLRRMVQIRTVF
                     LLLLLLFLWALSVNVSRGASSGLSFHLLGMTLAFLMLQPKAALGLLLVLSSVFMLPTV
                     WQHQQWLSLAPSMVLTVLPAWLVNVVLTPVLKRYLPKHVFVFILGHGFVTAGVSMLVT
                     GMCITWALHVTGRYSWYVLSEEALPVYVLLMWGEGFLTGLLVAVMVAFKPHWLDAYSD
                     AVYLPAAPVKRW"
     gene            complement(1648..2133)
                     /gene="mscL"
                     /locus_tag="PK856_09205"
     CDS             complement(1648..2133)
                     /gene="mscL"
                     /locus_tag="PK856_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HRL02584.1"
                     /translation="MVRKFGTELREFLMRGNVIDLAVGGVIGGAFGTIVKSLVDDVIM
                     PPIGLLLNGVDFSNLFIVLKDGAKGAGDYSTIAAAKAAGAVTLNYGLFINAIIQFMIV
                     GTAIFMLVRAVNKMHKPPVPEAAPETKDCPMCFTAIPVQAHRCPHCTSQLDGTTLDNA
                     H"
     gene            complement(2176..>2833)
                     /locus_tag="PK856_09210"
     CDS             complement(2176..>2833)
                     /locus_tag="PK856_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRL02585.1"
                     /translation="DRVRNLAAEFDSELVFHCDVASDDEINQLFVDLGKHWEGLDGLV
                     HAIAFAPKEALQGDFLDSLSRDAFNTAQDISAYSFPAMAKAARPMMKGRNSALLTLTY
                     LGAVRAIPNYNLMGMAKASLESSVRFTAAAVGKDGIRCNAISAGPIKTLAASGISNFS
                     RLLGHVASHNPLGRNVTTEEVGNVAAFLLSDLASGVTGEITYVDGGYSINALSVEEGQ
                     "
BASE COUNT          712 a          597 c          724 g          800 t
ORIGIN      
        1 agacatctgt ttcggatcaa aattaaacgg ctgattcaca tagttctcct caaattggaa
       61 ttgctactgt gttgctggta atgttaaaca tggccaaaag ctcagcttaa tgaagctctc
      121 aggacttctt ctagggaaag cgtatcatta aatcagcgct gctcaaaatg gtttttacgt
      181 tgaagctcaa ttttatcggt caccttgctt gtacttattg aataaaaggt atactgaatg
      241 ttttcttatt ggatgtctag tgatgacaga tgcattcttt gattggtcac tttggcgtcg
      301 cagtgtgggt gcggatttga gccaagtgca ataccccaat ttaaaagccc aattgcctta
      361 tttgcagcag atggacatca cagaatttaa acccgcagct gtgatggtat tgatagagcc
      421 aggcagcgtg gcacaagtgg tattgactgt gcgttctttg caattgcacc agcatggagg
      481 ccaagtgtct tttgccgggg gaaagaaaga tcctgaagac ggcaatctga tagaaactgc
      541 cttgcgcgaa acccatgagg aattgggcat accaccaaac aatattgagg tattggggca
      601 aatgcccagt ttgccaactg tcagtgccta caatgtttcc cctgtaatcg gcttgctcaa
      661 gccgcacacc aatatcgtgc cgaatgttgg cgaagtggct aaggtcttct tggtgccttt
      721 ggcgcaattg atgcaaccca aacggtattt gcattatccc atcatgcgtg gcaatcaggt
      781 atttcaaacc gtgaaattcg cgcataccga aaacatttgg ggagcaacag cgaccatttt
      841 attggcttta gctgagcatt accaagctta cgcaagagac acccgttatg gttttgatga
      901 atgaagatgt gccactgtgg ttggcttatg gctccattgc attgttgttg ctgatggtgg
      961 gttatgtgtg tcaggacaaa gctttgttaa ggcgcatggt tcaaatacgc actgtttttt
     1021 tgttgctatt gctgctgttt ttatgggcct taagcgtgaa cgtcagtcgt ggcgccagtt
     1081 ctggcttgag ttttcatttg ctgggcatga cactggcttt tttgatgttg caacctaagg
     1141 cggctctggg tttgttgctg gtgttgagta gcgtttttat gctgcccact gtttggcagc
     1201 atcagcaatg gctaagtttg gctccaagca tggtgctgac ggtgttgcct gcttggttgg
     1261 tcaatgtggt tttaacacct gtgctgaaac ggtatttacc caaacacgtg tttgtgttta
     1321 ttcttggcca tggttttgtc actgcgggtg ttagcatgtt ggtgacggga atgtgcatca
     1381 catgggcatt gcatgttaca ggccgctaca gttggtatgt attgagtgaa gaagcgttgc
     1441 ctgtgtatgt gctgttgatg tggggagagg gattcttaac agggttgttg gtggcggtga
     1501 tggtggcgtt taaaccgcat tggttggatg cttacagtga tgctgtgtat ttgccagctg
     1561 cacctgttaa acgttggtaa gcggcctgta agttgtttag ggcaaaataa aacccgcttg
     1621 agagcgagtt ttatttgttt gtcacgctca gtgtgcgtta tccaaagtgg tgccgtccaa
     1681 ttgcgaagtg caatgtgggc agcggtgtgc ttgtactgga atggcagtga aacacatagg
     1741 gcaatctttg gtttctggag ccgcttcagg tacaggtggt ttgtgcattt tattcacagc
     1801 acgcaccaac ataaagatgg cggtgcctac aatcataaat tgaatgatgg cattgatgaa
     1861 caaaccataa ttcaaagtca ctgcaccagc agctttggct gctgcaatgg tggagtaatc
     1921 acccgcacct ttggcaccgt cttttaagac gatgaataaa ttactgaaat caacgccatt
     1981 cagcaataag ccgataggag gcatgatgac atcgtcaacc agtgatttga cgatggtgcc
     2041 gaaagctccg ccaatcaccc cgccgactgc taggtcaatc acgttgccgc gcattaaaaa
     2101 ttcgcgtaat tccgttccaa atttacgtac catgatgaat aactcctact tgatgtgatg
     2161 tgaaatggga aggttctatt gaccttcttc aacgctcaat gcattgatgc tgtagccacc
     2221 atcgacataa gtaatctctc cggtgacgcc agaagctaaa tcagaaagca agaaggctgc
     2281 cacatttccc acttcttcag tagtcacgtt gcgacccaat gggttgtgtg aggcaacatg
     2341 gcccaaaagg cgactgaaat tggagatgcc agaggcagct aaggttttga ttggaccggc
     2401 agaaatggcg ttacaacgga tgccatcttt gccgactgct gctgcagtaa agcgaacaga
     2461 agattccaag ctggctttgg ccatgcccat caaattgtag ttagggatgg cacgcacagc
     2521 gcccaaatac gtcaatgtca acaaggcaga gttacggcct ttcatcattg gacgggccgc
     2581 tttggccata gcagggaagc tataggcaga aatgtcttga gccgtgttga aagcatcacg
     2641 gcttaaactg tctaaaaaat caccttgtaa ggcttcttta ggggcgaagg caattgcgtg
     2701 gaccaaacca tccaaacctt cccaatgttt gcccaaatca acaaacaatt ggttgatttc
     2761 gtcgtcgctg gcgacatcac aatgaaacac cagttctgaa tcaaattcag cggccaaatt
     2821 gcgtacgcgg tct
//
LOCUS       DAOMVK010000296         2828 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_10486_length_2828_cov_2.685900, whole genome shotgun sequence.
ACCESSION   DAOMVK010000296 DAOMVK010000000
VERSION     DAOMVK010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10486_length_2828_cov_2.685900"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PK856_09215"
     CDS             complement(<1..366)
                     /locus_tag="PK856_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007341276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02586.1"
                     /translation="MFNKAQNNSNETSVGPNIKQPPRLFTATQQIIANIEAHLGAPLL
                     CYWNSPRGSICGNDVLALYHLLEHIGNHDKIYVFIKSDGGSGIQALRMINLIRGHSKE
                     LVSLVPLECASAATMMAIGA"
     gene            complement(517..1566)
                     /gene="recA"
                     /locus_tag="PK856_09220"
     CDS             complement(517..1566)
                     /gene="recA"
                     /locus_tag="PK856_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRL02587.1"
                     /translation="MSEDKSKALAAALAQIEKNFGKGAIMKMDGSHKDENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIYGPESSGKTTMCLEAVAQCQKSGGVCAFIDAENAFDPI
                     YARKLGVKVEDLLVSQPDTGEQALEICDMLVRSGGVDMVVIDSVAALVPRAEIEGEMG
                     DSHVGLQARLMSQALRKLTGNIKRTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     ASVRLDIRRTGQIKKGDDILGNETRIKVVKNKVAPPFKQAEFDILYGEGVSLEGEIID
                     IGVKLGLVEKSGAWYSYNGNKIGQGKDNTRMWLKENPEIANEIEQKIRAEVGITAQIT
                     EGTLDETDGEEPQDS"
     gene            complement(1619..2041)
                     /locus_tag="PK856_09225"
     CDS             complement(1619..2041)
                     /locus_tag="PK856_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02588.1"
                     /translation="MKKPEEIIMAVLAALWVILTYFVLTYIGAHLETSLTITGLTLLW
                     CVVAFVFWQKNLMAWLWPVFIGLLIACWWPFWDWFAIKDVVDTSRIGDTLLMTKPWYA
                     TWAFKFAIAIVPTLIAYVAAWKLAQKRKQKAVSPTPLA"
     gene            complement(2080..>2828)
                     /locus_tag="PK856_09230"
     CDS             complement(2080..>2828)
                     /locus_tag="PK856_09230"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRL02589.1"
                     /translation="DTEAEEDTYSIFNFQRGTEAGLCMHAILEVTDFSLSAASQSERY
                     LAILEQYQIAPEWYPALYQMIDVTRLAKLDADVCLADLSRKQYLPEMGFVLRADDFNA
                     AEIGKWLQNSSLPVNMKSALQGVTFRDISGFLNGFIDLTCIASNGRVYVIDYKSNHLG
                     MQLGDYTERALNHAITDHRYYLQALIYALAVERYLQGRGFKAEAIHIRYLFLRGMQAE
                     SNQGIWRWDIAASELEKLTPNLLHDNIGTV"
BASE COUNT          713 a          697 c          673 g          745 t
ORIGIN      
        1 ggcaccgatg gccatcatgg tggcggcgga tgcacattcc aaaggcacga gcgagaccaa
       61 ttctttgctg tggccgcgga tgaggttgat catgcgcaag gcttggatgc cgctgccgcc
      121 atcgctcttg atgaaaacat agattttgtc gtggttgcct atgtgttcaa gcaagtggta
      181 gagcgccaac acatcgttgc cacaaatgct gccgcgtggg ctgttccaat aacacagcag
      241 cggtgcgccc aaatgcgctt caatattggc gatgatttgc tgggtggcgg taaacaaacg
      301 cggtggttgt ttgatgtttg ggccaacgct ggtttcattg ctgttattct gggctttgtt
      361 gaacatgaac actccaagta tgggctgtta aaacatgagc caccatcaaa acataaatac
      421 tctaggcggg caatagcttg cgtttaatgg cgagcgagca aaaaaaagcg gcctgtacat
      481 ttacaggccg cttattggag tattcaaaca gttcaattat gaatcttgcg gctcttcgcc
      541 gtcggtttca tccaaagtgc cttcggtgat ttgggcggtg atgcccactt cggcacggat
      601 tttttgctcg atttcattgg caatttcagg gttttctttc aaccacatgc gcgtgttgtc
      661 tttgccttgg ccaattttgt tgccattgta gctgtaccac gcgcctgatt tttcgaccaa
      721 gcccaatttg acaccgatgt cgatgatttc accttccaaa ctcacgcctt cgccatacaa
      781 aatgtcgaat tcggcttgtt tgaacggcgg cgcaacctta tttttcacca ctttgatgcg
      841 ggtttcattg cccaagatgt cgtcgccttt tttgatttgg ccagtacgac gaatgtccaa
      901 acgcacagac gcatagaatt tcaaggcatt tccaccggtg gtggtttctg gactgccgaa
      961 catcacgcca attttcatgc ggatttggtt gatgaacacc accaaggtgt tggtgcgctt
     1021 gatgttgcct gtgagtttgc gcaaggcttg gctcatcaag cgtgcttgca agcccacgtg
     1081 gctgtcgccc atttcgcctt cgatttcggc cctaggcacc aaggcagcaa ccgagtcaat
     1141 gacgaccatg tccacgccac cagagcgcac gagcatgtcg cagatttcca aagcttgctc
     1201 gcctgtgtca ggttgagaca ccaacaagtc ttcaactttg acgcccaatt tgcgcgcata
     1261 aatcggatcg aatgcgtttt ccgcgtcaat gaaagcacac acgccgccac ttttttggca
     1321 ttgcgccacg gcttccaaac acatggtggt tttaccagaa gattctggac cgtaaatttc
     1381 cactatgcga ccgcgtggca aaccacccac gcccaaagcc aaatccagac ccaaagagcc
     1441 agtggaaatc acttccaaat tttcatcttt gtggctgcca tccattttca tgatggcgcc
     1501 tttaccgaag tttttctcaa tttgcgccaa ggcggcggct aaggctttgc ttttgtcttc
     1561 tgacatacgt gcaatctttc tatatctggg cttttttcaa ttaggattaa gaaaaaagtt
     1621 aagccaaggg agtaggggat actgcttttt gcttgcgctt ttgcgccaat ttccatgcgg
     1681 caacataagc aatcaaggtt ggtacgatgg cgatggcaaa tttgaatgcc caagtggcat
     1741 accaaggttt ggtcatcaat aaggtgtcac cgatgcgaga ggtgtctacc acgtctttga
     1801 tggcgaacca atcccaaaat ggccaccagc aagcgatgag caagccaatg aacacaggcc
     1861 acagccacgc cattaaattc ttttgccaaa agacaaaggc caccacacac cacaataagg
     1921 tcaagccggt aatcgttaac gaggtctcaa gatgagcgcc gatgtaagtc aaaacaaaat
     1981 aagtcaaaat cacccacagt gccgcaagca cggccatgat gatctcttca ggctttttca
     2041 taagaatggt gtcctttgtg ttacttttta gagaaataat taaacagtgc cgatattgtc
     2101 atgaagcaag ttgggcgtca atttttccag ctcgctagcg gcgatgtccc agcgccaaat
     2161 gccttgattt gattcagctt gcataccgcg taaaaacagg taacggatgt gtatcgcttc
     2221 ggccttgaaa cctcgacctt gcaaatagcg ttccacagcg agggcgtaaa tcaaagcctg
     2281 caaataatag cggtggtcgg taatcgcatg gtttagcgca cgctcggtat agtcgcccaa
     2341 ttgcatgccc aaatgattgg atttgtaatc aatcacatac acgcggccat tgctggcgat
     2401 gcaggtcaaa tcaataaagc catttaaaaa accactgatg tctctaaaag tcacaccttg
     2461 taaggcagac ttcatattaa caggtaaact tgaattttgt aaccattttc caatttcagc
     2521 ggcattaaaa tcgtctgcac gcaacacaaa tcccatttca ggcaagtatt gcttgcggct
     2581 taagtcggcc aaacacacat cggcatcaag cttggccaaa cgggtaacgt ctatcatttg
     2641 gtagagggca ggataccatt cgggggcaat ttggtattgt tccaaaatgg ctagataacg
     2701 ctctgattgc gacgcggcgc ttaggctaaa gtcggttact tccaagatgg cgtgcatgca
     2761 caggcctgct tcggtgccgc gctggaagtt gaaaatgctg taggtatctt cttctgcctc
     2821 ggtgtctg
//
LOCUS       DAOMVK010000297         2821 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_10520_length_2821_cov_3.299349, whole genome shotgun sequence.
ACCESSION   DAOMVK010000297 DAOMVK010000000
VERSION     DAOMVK010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10520_length_2821_cov_3.299349"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..165
                     /locus_tag="PK856_09235"
     CDS             <1..165
                     /locus_tag="PK856_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HRL02590.1"
                     /translation="AHFDLRPKGIVKMLDLLRPIYRKTAAYGHFGREDEEFTWEKTDK
                     AAALRAAAGL"
     gene            493..1794
                     /locus_tag="PK856_09240"
     CDS             493..1794
                     /locus_tag="PK856_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL02591.1"
                     /translation="MSVATTESQPANSKGRVLFASLMGTTIEFFDFYIFATAAALVFP
                     HLFFPAGNEHAALLASLATFAAAFFARPIGSVIFGHFGDRIGRKATLVASLLTMGVST
                     VAIGLLPTYASAGLWAPALLTLCRIGQGLGLGGEWSGAVLMATENAPEGKRAWYGMFP
                     QLGAPIGFILSGGVFLLLGNILTEEQFFAWGWRVPFLFSALLVLMGLWMRLKLTETPA
                     FQKVLNSKKEAKVPFKQVVLEHPKTLILGIVAGICTFVVFYLTTVFAMAWGTTKLGYS
                     RNEFIIMELIATLFFALFIPLSAVMAEKFGRKNSSIFICVLAALFGLTFNPLLGSGSM
                     LGVLLFLCLGMTIMGLTYGPIGTVLSEIFPTSVRYTGSALTFNFAGIFGASFAPFIAT
                     SLATDHGLWAVGLYLSAASIISLVAFVLLRETKSVDVNNQI"
     gene            1989..2690
                     /locus_tag="PK856_09245"
     CDS             1989..2690
                     /locus_tag="PK856_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02592.1"
                     /translation="MKTWAIGIVASSMVLASTSALAASYANENQFAVDGKAMKVTETS
                     ARLLSNPEGTAPLLVEDITDGMGKTKIPGYKVMVMTRALSTAFDARKKDDGTIENLKT
                     VHRGGKILFGIPVKNGKMNVNEAKVLSLGLISDEGKTFSPNNKDTAPSGEQKFSDSSK
                     VTALSYKINSLTLPNLKAGERVGGGISYKLHTVVDGHTYDVSANTVFPSVETKGQPKH
                     LKAFPAKANVFEANR"
BASE COUNT          658 a          588 c          751 g          824 t
ORIGIN      
        1 gcgcactttg atttgcgccc taaaggcatc gtgaaaatgt tggatttgtt gcgcccgata
       61 taccgtaaaa ctgccgctta cggccacttt ggtcgcgaag acgaagagtt tacttgggaa
      121 aaaaccgaca aagccgcggc attgcgcgcc gctgcaggtt tataacactt tccatagcac
      181 cctgttcaaa cagccacact taaacggtgt ggctgttttg ttttaagcgg cctgtaaact
      241 ggaatggggc tttggggtgg gcagaaacag cctttttaga gtgtattgtg aataaatgtt
      301 aaatttacgc gggcttgtgg tgtgctgatg cggtgattat tcatatggtg gtgacttctg
      361 ccagtttgga tttaaatttt ggaattatca attttataag tatcaaattg ttgcgatgca
      421 atggattttt gtcatgtgaa atggcatgat attgctcata taaagaaatt catagaagaa
      481 agagaacgtt ttatgtcagt tgcaaccaca gaatcgcaac ctgccaattc caaaggccga
      541 gtgctgtttg cgagtttgat gggtaccacc atcgagtttt tcgattttta cattttcgcc
      601 actgcggcgg ccttggtgtt tccacatttg ttttttcctg caggcaatga gcatgcggct
      661 ttgttggcat cattggccac attcgctgcg gcattttttg cgcggcccat cggttcggtg
      721 attttcggtc actttggtga ccgcattggt cgcaaagcca cattggtggc ttcgctgttg
      781 acgatgggcg tgtctacggt ggcgataggc ttgttgccaa cctatgcttc agcaggcttg
      841 tgggcacctg ctttgttgac tttgtgccgc ataggccaag gtttgggttt gggcggtgag
      901 tggagtggtg cggtgttgat ggcgactgaa aatgcgccag aaggcaaacg ggcttggtat
      961 ggcatgttcc cgcaattggg cgcacccata ggctttattt tgtcgggcgg ggtgttcttg
     1021 ttattgggca atattctgac cgaagagcag ttttttgcct ggggttggcg cgtgccgttt
     1081 ttgttctcgg ctttgttggt gttgatgggc ttgtggatgc gcttgaaatt gactgaaacc
     1141 ccagccttcc aaaaagtgtt gaactcgaaa aaagaagcca aagtgccgtt caaacaagtg
     1201 gtattagagc atccaaaaac cttgattttg ggcattgtgg caggcatttg cacgtttgtg
     1261 gtgttttact tgaccacggt gtttgccatg gcatggggca cgaccaagct gggctattcg
     1321 cgcaatgaat tcatcatcat ggaattgata gccaccttgt tcttcgcttt gttcattcct
     1381 ttgtctgcgg tgatggcaga gaaatttggt cgcaaaaatt cgtctatttt catttgcgtg
     1441 ttggcggcct tgtttggcct gacgttcaac ccattgttgg gttcgggcag catgttgggc
     1501 gtgttgttgt tcttgtgttt gggcatgacc atcatgggtt tgacttacgg ccccatcggc
     1561 acggtattgt cagaaatttt ccccacttca gtgcgctaca ctggctcggc tttgacgttt
     1621 aactttgcgg gtatttttgg tgcatcgttt gcgcctttca tcgccaccag tttggccaca
     1681 gaccacggct tgtgggcagt gggtttgtat ttgtcggccg cttccattat ttcgttggtg
     1741 gcatttgtgc tgttgcgcga aaccaaaagc gtggatgtga acaatcaaat ctgattgaaa
     1801 accatttgaa aaagcggcct gtaaacgatt tacaggccgc ttttgcatgt tcagtcgctg
     1861 tagccatact ttggatatgg gcgtttgcct gtgacttgtt ggtttaattg tttgaagaaa
     1921 tgattttata gattataatc attctcgttt gtatttgact gcagcatcac attaaaaagg
     1981 gaatgggcat gaaaacttgg gccataggca tcgtcgccag cagcatggta ttggcaagca
     2041 cttcagcatt ggcggcatca tatgccaatg aaaatcagtt tgcggtggat ggcaaagcca
     2101 tgaaagtgac tgaaacctcg gcacgtttgt tgtccaatcc agaaggcacc gcacccttgt
     2161 tggtggaaga catcaccgat ggcatgggca aaaccaagat acctggctac aaagtgatgg
     2221 tgatgacccg cgctttgtct actgcttttg atgcacgaaa aaaagacgat ggcacgattg
     2281 aaaacctcaa aaccgtgcac cgtggtggca aaatattgtt cggcattcct gtgaaaaacg
     2341 gcaaaatgaa tgtgaacgaa gccaaagtat tgagcttggg cctcatcagt gacgaaggca
     2401 aaaccttctc acccaataac aaagacactg cgccatcagg cgagcaaaaa ttcagtgaca
     2461 gcagcaaagt gaccgccttg tcttacaaaa tcaacagcct gactttgcct aatttaaaag
     2521 caggtgagcg tgtgggcggt ggcatcagct acaaattgca caccgtggtt gatggccaca
     2581 cttacgatgt gtcggccaat acggtgtttc ctagcgtgga aaccaaaggc caaccgaaac
     2641 acttgaaagc cttccctgcg aaagccaatg tgtttgaagc caaccgttaa ttcgattttc
     2701 acagcgacct gtggtgttac aggccgcttt ttatttgagc taaagagagc attatgaaaa
     2761 aaacattgtt gagcagcgtt ttgggcttgg gtttgggttt ggcatcgttc agcgcgatgg
     2821 c
//
LOCUS       DAOMVK010000298         2806 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_10603_length_2806_cov_3.571792, whole genome shotgun sequence.
ACCESSION   DAOMVK010000298 DAOMVK010000000
VERSION     DAOMVK010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10603_length_2806_cov_3.571792"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="PK856_09250"
     CDS             <1..988
                     /locus_tag="PK856_09250"
                     /inference="COORDINATES: protein motif:HMM:NF013698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRL02593.1"
                     /translation="ISQLAYKGSLMYLGLDIAKAKIDCCLMHDTKSLHRTFENNQDGF
                     IKLTGWLEKHTANKVYACCEATGSYSEAIADYLHDTGHKIAVVNPLRIKAFVTSELQN
                     VKTDKQDAKSIAIYCQRNQPELYKPASPSERELKALTRYLDGLKELLTSHTNRSLVAH
                     HSVKPYIETSIKNTKAEIKVIEKVIQSRIKSDDDLDKQAMLLKSITAISDGTIPHILT
                     LFADKQFKTAKQATKYVGLNPIIKQSGNKSTRHLAISKMGDKYIRSALYMPAVVAFRM
                     AVYQPFIKRLKDKGKTNKQIICAMMRKLLVYCFTVLKTGNQFDPKTILNHQS"
     gene            complement(1253..2272)
                     /locus_tag="PK856_09255"
     CDS             complement(1253..2272)
                     /locus_tag="PK856_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02594.1"
                     /translation="MDAYDYMEQAADVGEKTLEGVRLIEQAIKAADAENDVEAGYEAR
                     DMLMDATYDLGMPKKQLMAFAWMIKQFEAEEIYIDEDDLMWKYKWVALGIVDFPEISK
                     AQIDHLLDDMKQKYLGFQYSLKPYYKIRTMMAMSMGEPEEVTRLRTEWKAAKKDYMND
                     CIACETNDEVYFQFYHGDYQKAVDKAKSLISGKQKCDEVPHLTNGVLALAFWQLGNAE
                     AAAERFKKGYKLTKNKAEFLNANADFIQYLTASEQWQEAEKVAQAELKVLPESENKFR
                     QFRLYAAIAVLLQAAQKQGMSLDVGMGLQAALQHAQQAATAFDERNGNTYYQQQLATQ
                     LAAYA"
     gene            complement(2336..>2806)
                     /locus_tag="PK856_09260"
     CDS             complement(2336..>2806)
                     /locus_tag="PK856_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02595.1"
                     /translation="LFQAEDVGFAFKPLFPEEARAFADLLSGLNRRLAAHQLEVVLNY
                     FSPHDIPMLYISSQQTQAERELERVAEASNDLFGGILGSLQEEEQAPLAQLYLNFHNE
                     VVQSVFCSGKSEATIASVVEVLYVQALLMGHYPLKQNEVKLMNQGLLSIIKAMK"
BASE COUNT          775 a          694 c          576 g          761 t
ORIGIN      
        1 aataagccag cttgcatata aggggagtct catgtattta ggtttagaca ttgcaaaggc
       61 aaagattgat tgttgtttaa tgcacgatac aaaatccctg caccgtacat tcgaaaacaa
      121 tcaagatggt ttcattaaac tgacaggttg gttagaaaaa cacaccgcaa acaaggttta
      181 cgcctgttgt gaggccacag ggtcatacag tgaagcgata gctgactatc tacacgatac
      241 aggccacaaa atagcagtag tcaatcctct acgcattaaa gctttcgtca ccagtgaatt
      301 gcaaaacgtc aaaaccgaca aacaagacgc taaaagcatt gcaatctact gccaacgcaa
      361 tcagcctgaa ctgtacaaac ctgcttcacc atctgagaga gaattaaaag ccctcacacg
      421 ctatttagat ggtttaaagg aattgctcac atctcatacc aatcgttccc tggtcgcaca
      481 tcattctgta aaaccttata tagaaaccag tattaaaaac accaaggcag aaatcaaagt
      541 gatagagaaa gtcatccagt cacgtattaa gtctgatgat gacttggata aacaggcaat
      601 gcttctaaaa tccatcacag caatcagcga cggcacaata ccgcacattc ttaccctgtt
      661 cgcagacaag cagtttaaaa ccgctaagca agccaccaag tacgttggtc ttaacccaat
      721 catcaagcaa tcaggcaaca agtctacaag gcatttagca atctcaaaga tgggcgataa
      781 gtacatacgt tcagcactgt acatgccagc cgttgtagcg ttcagaatgg ccgtatacca
      841 gccattcatc aaacgcttaa aagacaaggg taagaccaac aaacagatta tttgcgcgat
      901 gatgcgaaag ctcttggttt actgtttcac cgttctgaaa acaggtaatc agtttgaccc
      961 aaaaaccatc ctaaaccatc aatcgtgaaa ttaagtgtca agggcggcaa agccgtgcat
     1021 ttcacccttg acactttacc aaccaaaaaa cactcagccc aagggtaggt agttggcttt
     1081 ttaactgcct gcccttgcgg cgtcgcaagt gagacacgcc gtatgcatag aaacctaaca
     1141 atttctgcaa tattgactac aaaaaccaac gaattttaat aataatgttg actttatcaa
     1201 tacagtatct tttgtttctt aaatgtatct tttaaatcaa actcaaacac attcaggcat
     1261 aggctgccaa ttgtgtcgcc aattgctgtt ggtaatacgt gttgccgtta cgctcatcga
     1321 atgcggtggc ggcttgctgg gcgtgttgta atgcggcctg taaacccatg cccacatcca
     1381 aactcatgcc ttgcttttga gcggcctgta acaacaccgc aatggcggca tacaagcgaa
     1441 attggcggaa tttgttttcg ctttcaggca agactttcaa ttcggcttgg gcgacttttt
     1501 cggcctcttg ccattgctca ctggcagtca aatactgaat gaaatcggca ttggcattca
     1561 aaaattcggc tttgtttttg gtgagtttat agcctttttt aaagcgctca gctgcggctt
     1621 cggcattgcc caattgccaa aaggccaagg ccaacacgcc attggtcagg tgcggtactt
     1681 catcgcattt ttgcttgccc gaaatcaagg atttggcctt gtccacggct ttttgataat
     1741 cgccgtggta aaactgaaaa tacacttcat cgttggtttc gcaggcgatg caatcgttca
     1801 tatagtcttt tttcgctgcc ttccattcag tgcgcaagcg cgtcacttct tcaggctcgc
     1861 ccatgctcat ggccatcatg gtgcggattt tgtaatatgg cttcaatgag tattgaaagc
     1921 ccaggtattt ttgcttcatg tcgtctaaca aatggtcgat ttgcgctttg ctgatttcgg
     1981 ggaaatcgac tatgcccaag gccacccatt tgtacttcca catcaaatcg tcttcatcga
     2041 tgtagatttc ttcagcttca aactgcttaa tcatccatgc gaatgccatc aattgcttct
     2101 tgggcatgcc caaatcataa gtggcgtcca tcaacatgtc gcgcgcttca tagcctgctt
     2161 cgacatcgtt ttccgcatcg gcagccttaa tcgcttgctc aatcaagcgc acgccttcca
     2221 aagttttttc gcccacatca gcggcttgtt ccatgtaatc gtaagcgtcc atgcacagtc
     2281 tctttcattt gttggtttgc ttgcttttaa cagctgtttt aggtttacag gccgcttatt
     2341 tcatcgcttt gatgatgctg agcaagcctt gattcatcag cttcacttca ttttgtttca
     2401 atgggtaatg ccccatcaac aaggcttgca cgtacaacac ctcgaccaca ctggcgatgg
     2461 tggcctcgct cttgcccgag caaaacaccg attgcaccac ttcattgtga aaattcaaat
     2521 acagctgtgc caacggtgct tgttcttcct cttgcaaact gcccaagatg ccgccaaaca
     2581 agtcattgct ggcttcggcc acacgctcca attcgcgttc ggcttgggtt tgttggctgc
     2641 tgatgtagag catgggaatg tcgtgggggc tgaaataatt caacaccact tccaattggt
     2701 gcgcagccaa acggcggttc aggccgctta ataaatcagc aaacgcgcgt gcttcttcgg
     2761 gaaacaaagg cttgaaggca aaacccacgt cttcggcctg aaacag
//
LOCUS       DAOMVK010000299         2803 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_10619_length_2803_cov_2.824600, whole genome shotgun sequence.
ACCESSION   DAOMVK010000299 DAOMVK010000000
VERSION     DAOMVK010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10619_length_2803_cov_2.824600"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="PK856_09265"
     CDS             complement(<1..737)
                     /locus_tag="PK856_09265"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HRL02596.1"
                     /translation="MADTHYQTLGVTRDADADTIKKAYRKLARKYHPDLSKEPDAQER
                     MQDINIAYETLSDAEKKAEYDYLLDHPHAYTGGQSTGGSGFDSRAYEQHAHYGDGQDF
                     SGFEDLFGRFGAGFGGGNSRSRSQREPQAWQGEDQHASIEVDLQAAYTGSTEQIGLNI
                     PTYNAYGQAEIQRKTLQVKIPAGVKQGQSIRLAGQGQAGMNGGGNGDLYLEIHYRTPK
                     DVSVDGLDVLITTNVLPWEAAIGGPITV"
     gene            complement(942..1400)
                     /locus_tag="PK856_09270"
     CDS             complement(942..1400)
                     /locus_tag="PK856_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HRL02597.1"
                     /translation="MPALQFESSSLVLVALIGLGVLSHNASITIAACVLLLCQQTALA
                     QYLPWIQKNGLNIGIIILTIGVLAPLASGSLKVPSLMAIFTNWQLLAAVAVGILVAWL
                     GGRGVGLMSAQPLLVTGLLLGTIIGVAVFRGVPVGPLIAAGILSLVLQAQ"
     gene            complement(1509..2324)
                     /locus_tag="PK856_09275"
     CDS             complement(1509..2324)
                     /locus_tag="PK856_09275"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HRL02598.1"
                     /translation="MTASVFEIDPQLVTDFARDGVVVVRGLVKPEMIELLARGIDKNI
                     AEPSPRAKTASRSDDPGWFFEDFCNWQHIPEYREFIFNTPIAEAAAQLMQSEQVRLYH
                     DHTLVKTAGTQQPTPWHQDQPYYNIDGRQNISMWLPVDPIPRAATLEFVGGSHLGPWL
                     MPRSFMDKQAKWFPEGSLQDLPDIEADRSAHNILGWELQPGDAVFFHMLALHSSKGSA
                     TTRRVFSVRFLGDDITHAPRNWVTSPEFLGLAERLPAGAPMHDDELFPLLYSA"
     gene            complement(2337..>2803)
                     /locus_tag="PK856_09280"
     CDS             complement(2337..>2803)
                     /locus_tag="PK856_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HRL02599.1"
                     /translation="TQGMRLMAMVGFIMITATGFAAVMTATGDVPALVESSVGIIGDN
                     KGLAAFMMLLIGLFVTMGIGSSFSTVPILTAIYVPLCVSFGFSPMATVAIIGAAGALG
                     DAGSPASDSTLGPTAGLNADGQHDHMWDTVVPTFLHYNLPLICTGWIAAMVL"
BASE COUNT          660 a          713 c          786 g          644 t
ORIGIN      
        1 actgtgatgg gcccgccgat ggctgcttcc catggcaaca cattggtggt gatcaacaca
       61 tccaagccat ccacgctcac gtctttgggg gtgcggtaat ggatttccaa atacaaatca
      121 ccattgccac caccattcat gcccgcttga ccttggcccg ccaaacggat gctttggcct
      181 tgtttcacgc ctgcggggat tttgacttgc aaggttttgc gctggatttc ggcttggcca
      241 taggcattgt aagtggggat gttgaggcca atttgttcgg tgctgcctgt gtatgcggcc
      301 tgtaaatcaa cttcgatgct ggcgtgttgg tcttcacctt gccaagcctg tggttcacgt
      361 tggctgcgag aacggctgtt gccgccgcca aagcccgcgc caaagcggcc aaacaaatct
      421 tcaaaaccac tgaagtcttg gccatcgcca taatgggcat gttgttcata ggcgcggctg
      481 tcaaagcctg agccgcctgt tgactggcca cctgtgtagg catgcgggtg gtcgagcagg
      541 taatcgtatt cggctttttt ttcagcatcg ctcaaggttt cgtaggcaat gttgatgtct
      601 tgcatgcgct cttgcgcatc gggttctttg cttaaatcgg ggtggtattt gcgcgccagt
      661 ttgcggtagg cttttttgat ggtgtcggca tctgcatcgc gtgtcacacc gagggtttgg
      721 taatgggtgt ctgccatgtg tcttaaatcc tgcaaaaaaa gatgaatggg aaaacctgtg
      781 tttgtgagta tataaccaag cgctgtactg atgaaacacc acagtcaaat aaatcgcggt
      841 ttgtcatgag gatggcgtgt catcttgatg gaacacaaac aagcggcctg taaacgttta
      901 caggccgctt gcacattaca gaaacttatt gtgaaagaag cttattgcgc ttgtaatacc
      961 aaagacaaaa tgcctgcagc aatcaatggc cccacgggca cgccgcgaaa cacggccacg
     1021 ccgatgatgg tgccgagcag caagcctgtc accaacaagg gttgggcgct catcaagcca
     1081 acgccgcgtc cgcccagcca tgcgaccaag atgcccacag ccacagccgc gagcaattgc
     1141 caattggtga agatggccat caagctgggc actttcaagc tgcccgaggc cagtggcgcc
     1201 aacacgccta tggtgagaat gatgatgccg atgttgaggc cgtttttttg tatccaaggc
     1261 aggtattgcg ccagcgcagt ttgttggcac agcaacaaca cgcaggcggc gatggtgatg
     1321 gaggcgttgt ggctcaatac gcccaggcca attaaggcca ccaataccaa agaactgctt
     1381 tcaaattgga gggcaggcat ggtctgactt ttctcaatga aaacgccatg gtaacagcga
     1441 tgctgacaaa gaaacaggct gccagcatca taactggcag cctgtttttg tatgcacatc
     1501 tggtggtatt aagcgctgta aagcagtgga aacagttcgt catcgtgcat gggcgcaccc
     1561 gcaggcaagc gctctgccaa gcctaggaat tcaggcgagg tcacccaatt gcgtggggca
     1621 tgggtgatgt cgtcgcccaa gaagcgcacc gagaacacgc ggcgcgtggt ggccgagcct
     1681 ttggatgagt gcagggcaag catgtggaag aacactgcgt cccctggttg caactcccaa
     1741 cccaaaatat tgtgggcgct gcgatcggct tcaatgtcag gcaaatcttg taagctgcct
     1801 tctgggaacc atttggcctg tttgtccatg aatgaacgtg gcatcaacca aggccctaag
     1861 tgtgagccgc caacaaattc cagtgtggcg gcgcggggaa tggggtcgac aggcaaccac
     1921 atgctgatgt tttggcggcc atcgatattg taatacggtt ggtcttggtg ccacggtgtg
     1981 ggctgctgtg tgcccgcggt tttgaccaag gtgtggtcgt ggtacaggcg cacttgttcg
     2041 ctttgcatca attgtgcggc ggcttcggca atgggcgtgt tgaaaatgaa ttcacggtat
     2101 tcggggatgt gttgccaatt gcaaaagtct tcaaaaaacc aacctggatc gtcgctgcga
     2161 ctggcggttt tggcacgagg gctgggttcg gcaatgtttt tgtctatgcc acgggcaagc
     2221 agttctatca tttctggctt gaccaagcca cgtaccacca ccaccccgtc gcgggcgaag
     2281 tcggtaacca attggggatc tatttcaaat actgaggcag tcatgaggcg tttcctttac
     2341 agaaccatgg cggctatcca gccggtacaa atcaacggta aattgtagtg taaaaatgtg
     2401 ggtaccaccg tgtcccacat atggtcgtgc tgtccatcgg cgttcaggcc ggctgtgggg
     2461 cctaaggttg aatctgaggc aggagagcct gcatcaccca aagcgccagc ggcaccgata
     2521 atcgccacgg ttgccatcgg tgagaagcca aacgacacac acaaaggcac ataaatcgcg
     2581 gtcaaaatcg gtacggtaga gaatgaagaa cctatgccca tggtgacaaa caagccaatg
     2641 agcaacatca tgaatgcggc caaaccttta ttatcaccaa taatgcccac tgaagattcc
     2701 accaaagcag ggacatcgcc cgtggccgtc atcaccgcgg caaagcccgt ggcggtaatc
     2761 atgatgaagc ccaccatcgc catgaggcgc atgccttgcg tga
//
LOCUS       DAOMVK010000300         2800 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_10642_length_2800_cov_2.631694, whole genome shotgun sequence.
ACCESSION   DAOMVK010000300 DAOMVK010000000
VERSION     DAOMVK010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10642_length_2800_cov_2.631694"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..752
                     /gene="rpoE"
                     /locus_tag="PK856_09285"
     CDS             153..752
                     /gene="rpoE"
                     /locus_tag="PK856_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HRL02600.1"
                     /translation="MDERQIDQELVERAQQGDTKAFEILVAKYNRRLGRLLSRFIKDE
                     HEVNDVVQESFIKAYRALPNFRGDSAFYTWLYRISINTAKNFLSNNNKRPIMSSEITN
                     GDGEVMDVVEQVADNLTPEAELMNKQILQTVESAVAKLPEDLRRAITLREMDGLSYEE
                     IAEVMQCPIGTVRSRIFRAREVIAADLRPLLDTSEHNRW"
     gene            918..1760
                     /gene="dapF"
                     /locus_tag="PK856_09290"
     CDS             918..1760
                     /gene="dapF"
                     /locus_tag="PK856_09290"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRL02601.1"
                     /translation="MRTLNFTKMHGLGNDFIVINAMNKPIDWTAEHVNAWSNRHTGIG
                     FDQLLVIETSENERCDFKYRIFNADGGEVEQCGNGARCFAKYVTEHGLTDKTSVMVET
                     MRGIIVLNVLADGQITVDMGKPHFAPEEIPYIPKNKADHDAKVHMLVVGMETVPVSLV
                     SMGNPHAVMLVEDVATAPVQVLGEAIEKHPQFPQRVNVGFGQVISPSEINLRVFERGV
                     GETQACGTGACAAVVAGISHGLLTDTVLVHLPGGDLTIHWKEGESVMMTGPAAEVFTG
                     SLSY"
     gene            1760..2389
                     /locus_tag="PK856_09295"
     CDS             1760..2389
                     /locus_tag="PK856_09295"
                     /inference="COORDINATES: protein motif:HMM:NF016243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HRL02602.1"
                     /translation="MSMNEQSVREYLLQHPDFMQRYPDVLPVAKGKVKDFRDAQLAKM
                     QADNQLLKQRQQQWVNALHDNQHLSSVFWQHAADLARCNSYKQVLAVLEHMLFEELDF
                     PIYALKLMPPSNKRPVPEENRLQAGFKWIETQAWACAHLPKSMNTWLPEAYESHLVLP
                     LLRQEQFLGVWVLASKQVDYFHPEQDTTYLKAFVQVLSATLARIMGVAD"
     gene            2389..>2800
                     /locus_tag="PK856_09300"
     CDS             2389..>2800
                     /locus_tag="PK856_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02603.1"
                     /translation="MLSTVVVCVYVGLFVYFTVRSGLRQWLSGALVLAAGGSWLLSNL
                     LPGFVYYHGSAFLNLAVLYITLSSLFFFVNHWHYHSATGVFYAEPDTPPYLVYLAVSG
                     MMMHLAWLIPLLWSTYQYPEGLSSFSLLGLLQLYF"
BASE COUNT          707 a          597 c          738 g          758 t
ORIGIN      
        1 gtggtgtttg ctgcgcaatt gaatgaccag catttgcgta agaaccatcc tgccacccaa
       61 cctgctttgg cgtttttaaa acaatacaca atagtccgac acccacgcat tgataacagt
      121 gcttaaaacc aataggtaag tgataaaaca ccatggatga aagacaaatc gatcaagaat
      181 tggttgagcg agcgcagcaa ggagacacca aagcttttga aattttggtg gccaaataca
      241 accgccgttt ggggcgtttg ctgtcacgct tcatcaaaga tgaacatgag gtgaacgatg
      301 tggttcaaga atcattcatc aaggcttatc gtgcgttgcc caactttcga ggcgacagtg
      361 ctttttatac ttggttatac cgaatcagca tcaataccgc gaaaaacttc ctgtccaata
      421 acaacaagcg tcccatcatg tccagcgaga ttaccaatgg tgatggtgaa gtaatggatg
      481 tggtggagca ggtggcggac aatttaaccc ctgaagccga gttgatgaac aagcaaattt
      541 tgcaaacagt agaaagtgct gtggcaaaat tgccagagga tttgcgccgt gccatcacct
      601 tgcgtgaaat ggatggtttg tcgtacgaag aaatcgccga ggtgatgcaa tgtccgatag
      661 gcacagtgcg ttcgcgcatt ttccgtgcgc gtgaggtcat tgcggcggat ttgcgaccgc
      721 tattagacac gagtgaacac aaccgttggt aagttgtttc acgtttacag gccgcttgta
      781 tgctcataca agcggcctgt aaacatttgt ggtcatgata aagccgtaat ccttgtgaat
      841 cgtgtggcag acactgtgct gacgtggcaa tttatgccac aatgaaagtc ttcttgcttt
      901 ggcctacggt taataccatg agaacattga atttcaccaa aatgcacgga ttgggcaacg
      961 attttatcgt catcaatgcc atgaataagc ccatagattg gactgccgaa cacgttaatg
     1021 cgtggtcgaa tcgccatacg ggcatcggtt ttgaccaatt gttggtcatt gaaacctctg
     1081 aaaatgagcg ttgtgatttt aaataccgca tcttcaatgc cgatggtggt gaagtggagc
     1141 aatgtggcaa tggtgcacgt tgttttgcca aatacgtgac cgagcacggt ttaaccgata
     1201 aaacctcggt aatggtggaa accatgcgcg gcatcattgt gctgaatgtg ttggcagatg
     1261 gccaaatcac cgtagacatg ggcaaaccac attttgcgcc agaagaaatt ccctacatac
     1321 ccaaaaacaa agccgaccac gatgccaaag tgcacatgtt ggtagtgggc atggaaaccg
     1381 tacctgtcag cttggtgagc atgggcaatc cgcatgcggt gatgttggtg gaagatgtcg
     1441 ccactgcacc cgtgcaagtc ttgggtgagg ccatagaaaa acacccgcaa ttcccacaac
     1501 gggtgaatgt gggttttggc caagtcattt ctccttcaga aatcaatttg cgtgtgtttg
     1561 agcgcggtgt gggtgaaacc caagcctgtg gcacaggtgc atgcgcggct gtggttgcgg
     1621 gcatcagtca tggcttgttg accgacacgg tattggtaca cctgcctggc ggggatttga
     1681 ccatacattg gaaagagggc gagtcggtga tgatgacggg ccctgccgct gaagttttca
     1741 caggaagttt gagttattga tgagcatgaa cgaacaaagc gtgcgtgagt atctgttaca
     1801 acacccagat ttcatgcagc gatatcccga tgtgttgccc gtggccaaag gcaaggtgaa
     1861 agattttcgc gatgcccaat tggccaaaat gcaagccgac aaccaattgc tcaagcagcg
     1921 tcagcaacaa tgggtcaatg ccttacatga taatcagcat ctgtcctcgg tgttttggca
     1981 acatgctgcc gatttggccc gttgtaatag ctacaaacag gtattagcag tattggagca
     2041 catgttgttc gaggagttgg attttccaat ttacgcttta aaattgatgc caccgagcaa
     2101 taagcggcct gtacctgaag aaaaccgttt acaggccggg tttaaatgga tagaaaccca
     2161 agcttgggct tgtgcccatt tgcccaaaag catgaacact tggttgccag aagcctatga
     2221 aagccatttg gtattaccat tgttgcgcca agagcagttt ctgggtgtgt gggtattggc
     2281 ttctaagcaa gtggattatt tccacccaga gcaagacacc acttatttaa aggcttttgt
     2341 gcaggtgttg tctgccacat tggcacgcat catgggtgtg gccgattgat gttaagtacg
     2401 gtcgtggtgt gtgtgtatgt gggcttgttt gtctacttta cggtgcgcag tggtttaaga
     2461 caatggctga gtggtgcgct ggtcttggct gcgggcggct cttggctgct cagtaattta
     2521 ttgcctggct ttgtgtatta tcacggttct gcgtttttga atttggcggt gttgtacatc
     2581 accttaagca gtttgttctt ttttgtgaac cattggcatt accattcggc cacaggcgtg
     2641 ttttatgcag aacccgatac gccgccttat ttggtgtatt tggccgtcag tggcatgatg
     2701 atgcatttgg cgtggttgat accgctgttg tggtcaactt accaatatcc tgaaggactg
     2761 agcagctttt ctttattggg tttgctgcaa ttgtatttct
//
LOCUS       DAOMVK010000301         2794 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_10667_length_2794_cov_3.686747, whole genome shotgun sequence.
ACCESSION   DAOMVK010000301 DAOMVK010000000
VERSION     DAOMVK010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10667_length_2794_cov_3.686747"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..389)
                     /locus_tag="PK856_09305"
     CDS             complement(147..389)
                     /locus_tag="PK856_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HRL02604.1"
                     /translation="MLEMRPNCECCDAALPANSERAYICSFECTFCASCATGHLDAHC
                     PNCGGQLRLRPSRAPHLWDDFPASTEHVYKPDCGQA"
     gene            complement(524..1969)
                     /locus_tag="PK856_09310"
     CDS             complement(524..1969)
                     /locus_tag="PK856_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRL02605.1"
                     /translation="MTQLLDYRQFYINGLWVNPAKANDLEVIDPSTEKAVAVISIGDK
                     ADTDAAVAAAKAAFPAWAATAPAERLAYVEKILDIYKRRAKDMARAISLEMGAPQDMS
                     LEDQVDCGVYHIENFITAFKDFKFVRPLSADTPDTMIAWEPVGVVASITPWNWPMNQV
                     TLKVIPAILAGDTVILKPSEIAPLSSIVWTEIVDEAGLPAGVYNMVNGDGMGVGTQLS
                     THPDVDMVSFTGSTRAGVAITKAAADTVKKVVLELGGKGANLVFADADENAVARGVQH
                     CFYNSGQSCNAPTRMLVERSFYDKAVEIAKKVADETAVDIAHKEGAHIGPVISKLQFD
                     KIQGLIQDGINEGARLVSGGTGLPAHLSEGYFVRPTVFADVNNEMGIAKNEIFGPVLT
                     IIPFDTEEEAIAIANDTIYGLTNYVQTTDLERRRRLSRQLQAGMVEMNGKSRARGAAF
                     GGVKASGRAREGGVFGLEEFMDSKFISGWAE"
     gene            complement(2211..>2794)
                     /locus_tag="PK856_09315"
     CDS             complement(2211..>2794)
                     /locus_tag="PK856_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRL02606.1"
                     /translation="MAVFFNALNSWLFFKQKPSPRFAPAACIGLLGMVLLFWHELDFG
                     AWHVGQLTGMALAALGTFGFSLGNMLSVRHQKHGLDVFTTNAYAMLYGTLILAMLAAV
                     FDVSLPMPMQAGFVWPLLYLAVFGSVIGFTAYFTLVKRLGAAQSAYTTLLFPLVALSI
                     STLWEGYEWSVAAVLGMGLILAGNFWMFYKPKA"
BASE COUNT          751 a          766 c          632 g          645 t
ORIGIN      
        1 caaggcttgc gcgacaaaat tgagcaaacg catgtcgtgt ggcaggaagt ttgaccatat
       61 tgtaaacagg tcatacagat aacaatattg accatacagc tgtttacagg ccgcttaagc
      121 ggcctgtaaa cattccaata ttcaaattac gcttgcccac aatcaggctt gtacacatgc
      181 tcggttgagg caggaaaatc gtcccacaaa tgcggggcgc ggctggggcg caagcgcaat
      241 tgcccaccac aattggggca atgcgcgtcc aaatgccctg tggcgcaaga ggcgcaaaac
      301 gtgcattcaa acgagcaaat gtaagcccgc tcagaattgg caggcaaggc agcatcacaa
      361 cattcacaat ttgggcgcat ttccaacatg atgagtcctt gtatgggttt ggatacagtt
      421 tgattgtaat ggcattgtgt gtttattcaa gcatttgcat tgcatgcaga caaaaaaaat
      481 acaggccgca tcaagcggcc tataagctta ttcaagcaca gacttattca gcccagcctg
      541 aaatgaattt gctgtccata aattcttcca agccaaacac accgccttca cgagcgcggc
      601 ctgaggcttt gacaccacca aacgcagcac cacgggcgcg gctcttgcca ttcatttcca
      661 ccatgccggc ctgtaattgg cggctcaatc gacggcggcg ttccaagtcg gtggtttgca
      721 cgtaattggt caagccgtaa atggtgtcgt tggcaatcgc tatcgcttct tcttcggtat
      781 cgaatggaat gatggtcaac acagggccaa agatttcgtt tttggcaatg cccatttcat
      841 tgttcacatc ggcaaacacg gttgggcgca cgaaataacc ttcgctcaaa tgcgcaggca
      901 aacccgtacc gccagacacc aaacgcgcac cttcgttgat gccgtcttga atcaagcctt
      961 ggattttgtc gaactgcaat ttggaaatca caggaccaat gtgcgcacct tctttatggg
     1021 caatgtcaac ggcggtttca tcagccactt ttttggcgat ttccaccgct ttgtcgtaga
     1081 acgagcgctc aaccaacatg cgcgttggcg cattgcaaga ttggcctgag ttgtagaagc
     1141 aatgctgtac accgcgtgcc acggcgtttt catcggcatc ggcaaacacc aagttcgcgc
     1201 ccttgccgcc caattccaac accacttttt tcacggtatc ggcagcggct ttggtaatcg
     1261 ccacacctgc acgggtagaa cccgtgaacg acaccatatc cacatcagga tgggtagaca
     1321 attgcgtgcc cacgcccatg ccatcaccgt taaccatgtt gtaaacgcct gcaggcaaac
     1381 ctgcttcgtc cacaatctcc gtccacacga tagaagacaa aggcgcgatt tctgaaggtt
     1441 tcaaaatcac cgtgtcgcct gccaaaatcg caggaatgac tttcaaagtc acttggttca
     1501 tcggccagtt ccacggggtg atggaagcca ccacacccac aggttcccat gcaatcatgg
     1561 tatctggggt atcggcgctc aatggacgta caaatttgaa gtctttaaaa gcagtgatga
     1621 agttttcgat gtggtagaca ccgcaatcga cttggtcctc taaagacatg tcttgtggcg
     1681 cacccatttc caagctgatg gcacgggcca tatctttggc gcggcgtttg tagatgtcta
     1741 aaatcttttc cacataagcc aagcgctcag caggcgcagt ggctgcccaa gcagggaatg
     1801 cggctttggc ggctgccacg gctgcatcgg tatcggcttt gtcaccaatc gaaatcaccg
     1861 ccaccgcttt ttcagtagaa gggtctatca cttccaaatc atttgctttg gcaggattca
     1921 cccatagtcc attgatgtaa aactgacggt aatccaataa ttgtgtcatg tatttctcct
     1981 ctgttttttc gcacttggat gtttgctgcg gttttgtttc gcctaacaaa acaaagcctt
     2041 gctgctgtaa tcttcggtaa cttattcaat caaaatcagt ttgtcacaag ccacaccacg
     2101 atacctgaga aggtttgccg cagcaaccac ccccacccac ttcttacact tgctcacatt
     2161 cagaggcttg attcgattaa gaagaaaggc ttacaggccg caatgtaaat ttatgccttt
     2221 ggtttataaa acatccaaaa attacctgcc aaaatcaagc ccatacccaa cacagcagcc
     2281 acactccatt catacccttc ccacagcgtc gaaatgctca aggccaccaa ggggaacaac
     2341 agcgtcgtgt aagccgattg cgccgcgccc aggcgtttga ccagcgtgaa atacgcggta
     2401 aagccgatta ctgagccaaa caccgccaaa tacagtaaag gccacacaaa gcctgcttgc
     2461 atcggcatgg gcaaactcac atcaaatact gctgccaaca tcgccaaaat caacgtaccg
     2521 tacaacattg catatgcatt ggtggtgaac acatccaagc cgtgcttttg atggcgcacg
     2581 ctgagcatat tgcccaagga aaaaccaaac gttcccaagg ccgccaaagc catgcccgtg
     2641 agctgaccca cgtgccaagc gccaaaatcc agctcatgcc aaaacagcaa caccatgccc
     2701 agcaagccta tgcaagcggc aggtgcaaac cgtgggctag gcttttgttt gaaaaacagc
     2761 cacgaattga gggcattgaa aaacaccgcc atcg
//
LOCUS       DAOMVK010000302         2785 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_10714_length_2785_cov_3.273993, whole genome shotgun sequence.
ACCESSION   DAOMVK010000302 DAOMVK010000000
VERSION     DAOMVK010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10714_length_2785_cov_3.273993"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="PK856_09320"
     CDS             complement(<1..529)
                     /locus_tag="PK856_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRL02607.1"
                     /translation="MAAYYRFISRSTDADGVCVAHYEPLLPAQGAWNAHEQHMAPATG
                     VMCAELEAFLPRNDLRIGRVGLDILGLIPLQPFTISTKVLRPGKTIELIEATMAAQGR
                     ACIVARAWRMQTGDTSAIAGVEDRAIKRPEDMQDWTGMKQWAGGFIESMQFKADDTRR
                     AGKGVVWLNNDLDMVD"
     gene            749..2035
                     /locus_tag="PK856_09325"
     CDS             749..2035
                     /locus_tag="PK856_09325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRL02608.1"
                     /translation="MFASSSQHIASYYAQSATWPEFPTLQDALDCEILIIGAGFSGLH
                     TALKLAEAGVQNIAVIEASRIAWAASGRNGGQAILGWSSDMGPIVHDLGYGEALALWQ
                     DMEWAAQEIQDLPKRHGFDIDYRSGHLWCSVQAKKVTGLQHWVEEAQEKWHYEGLRFI
                     PKAELPHWIASERYQAAVLDQNGGHLHPLKLCYGMATSLQKHGVQLFEQTRALRYQRE
                     QDHIVVTTESGRITCRQLVLACNAYVDQLAPQLNQKILPVGCFQVATEVLSEECLQAA
                     LPHNSCVTDNQFILDYFRRSVDNRLLFGGGCTYLGGMPKDINAFMRPLLARVFPQLAD
                     VNFEFAWGGHLDCSVRRTPDVGMDGNVYWLQGYSGHGVLPTLAAARAVAEAILGKTER
                     IKRYQNIANPTFWGNEPLAAPIEALSKLYFRLRDRF"
     gene            complement(2162..2683)
                     /locus_tag="PK856_09330"
     CDS             complement(2162..2683)
                     /locus_tag="PK856_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02609.1"
                     /translation="MFKKTCIAVTALAALVIGLHWHSKAENASPTVAQVPQTLSKLPV
                     NSAQASSPTLTRAVLEQLGFKILSWEEDHDGLSISVQPKQAPSQAELYVQRMDLNAEA
                     CADEVDLGDSRIETINAAISVYHQQDPQSKVSPSVMVHNGKQTCWLIEGNSGLSAKQD
                     LASKLSLAKKLPL"
BASE COUNT          645 a          718 c          697 g          725 t
ORIGIN      
        1 catctaccat gtccaaatca ttgttcagcc acaccacacc tttgccagcg cggcgcgtgt
       61 catcggcttt gaattgcata gactcaatga agccaccagc ccattgcttc atgcctgtcc
      121 aatcttgcat gtcttcaggc cgcttgatgg ctctgtcttc aacacccgca atggcgctgg
      181 tgtcacccgt ttgcatgcgc cacgcccgtg ccacaataca ggcgcggcct tgtgcggcca
      241 tggtcgcctc aatcaactca atggtttttc ctggtcgcaa gactttggtg ctgatggtaa
      301 acggctgcaa aggaatcaaa cccaaaatgt ccaagcccac acggcctatg cgcaaatcat
      361 tgcggggcaa aaaagcctcc aattccgcgc acatcacacc cgtagcgggc gccatgtgtt
      421 gctcatgtgc attccaagca ccttgtgcgg gcaataaagg ttcgtaatgg gcgacacata
      481 cgccatccgc atctgtactg cggctgatga aacgataata agctgccatg gtgggtcctt
      541 tttaatgtct ggttctgtct tgccacagtc ttactggttg gtgaaatact aacaccgata
      601 ttggctgcgt ttcaaattta tgaacaatga ttcatcaatc aactttttcc tgctatctgt
      661 gcagtgcaaa acactgcaca gatgtgtttt tgtgacttct tcagtacact gattgacttc
      721 atgacaaacc gatatcaagg cgatttctat gtttgcctct tcaagccaac acattgcatc
      781 gtattatgcc caaagcgcca catggcccga atttcctacc ttacaagatg cgctggactg
      841 tgaaatttta atcataggag cgggtttcag cggcctgcat acggcgctaa aactggccga
      901 agcaggtgtt caaaacatcg ctgtgattga agccagtcgc atcgcttggg ccgcttcagg
      961 ccgcaatggt gggcaggcga ttttgggttg gtcatctgac atggggccta ttgtgcatga
     1021 tttgggctat ggcgaagcct tggctttgtg gcaagacatg gaatgggcgg cgcaagaaat
     1081 ccaagactta cccaagcgcc acggctttga catcgattac cgcagcggcc atttgtggtg
     1141 ctcggtgcag gccaaaaagg tcacaggctt acaacactgg gttgaggaag cacaagaaaa
     1201 atggcattac gaggggctgc gtttcattcc caaagccgaa ttaccgcatt ggattgcttc
     1261 tgagcgctat caagctgcgg tattagacca aaatggcggc cacttacatc ctttgaaact
     1321 ctgttatggc atggccactt ccttgcaaaa acacggcgtg caattgtttg aacaaacccg
     1381 tgctttgcgt taccaacgcg aacaagacca catcgtggtg acaaccgaat ctgggcgcat
     1441 tacctgtcga caattggttt tggcttgcaa tgcctatgtt gaccaacttg cgccccaact
     1501 gaatcaaaaa atcctgcctg tgggctgttt tcaagtggcg actgaagtgt tgtccgaaga
     1561 gtgtttacag gccgccttgc cccacaattc atgcgtgacc gacaatcagt ttattttgga
     1621 ttacttccgc cgcagtgtcg acaaccgctt gctgtttggt ggcggttgta cgtatttagg
     1681 cggcatgccc aaagacatca atgcctttat gcgccccttg ctggcacggg tgtttccaca
     1741 attggcggat gtgaattttg aatttgcttg gggtgggcat ctcgattgca gcgtgcgtcg
     1801 caccccagat gtgggtatgg atggcaatgt gtactggttg caaggctatt caggtcacgg
     1861 cgtgttgccg accttggccg ccgctcgtgc cgtggccgaa gccattttgg gaaaaaccga
     1921 acgcattaaa cgctaccaaa acattgccaa ccctacgttt tggggcaatg aacccctagc
     1981 cgcccccata gaagccttga gcaaactgta tttccgcctg cgcgaccgct tctaaaccat
     2041 ttgcctcatc aaagcggcct gtaaccgcat gatggactgt tacaggccgc tttgagtagt
     2101 gtgttcaggc ttccattcat ctaatagtaa tagccatcct taaggctaca aacttacctt
     2161 tctacaaagg cagttttttg gccaaactga gtttgctggc caagtcttgt ttggcgctca
     2221 agccactgtt gccttcaatc agccaacacg tttgtttgcc gttgtgcacc atcacgctcg
     2281 ggctgacttt ggattggggg tcttgttggt ggtaaaccga gatggccgca ttgatggttt
     2341 cgatgcggct gtcgcccaaa tccacttcat ctgcacaggc ttctgcattc aaatccatgc
     2401 gctgcacata caattcggct tggctgggag cctgtttggg ctgtactgaa atgcttaaac
     2461 cgtcgtgatc ttcttcccaa ctgaggattt taaaacccaa ttgttctaag acagcgcggg
     2521 tcaaagtggg agaacttgct tgggcactgt ttacaggcag cttactgaga gtttggggca
     2581 cttgagcgac ggtaggcgag gcattttcag ctttgctgtg ccaatgcaaa ccgatgacaa
     2641 gtgcggccaa ggctgtgaca gcgatgcatg tttttttgaa cataggccat cttgaggctg
     2701 gatacaaaga gaatggcgca cccaacagga atcgaacctg tggcctccag cttcggaaac
     2761 tgacgctctt ccaactgagc tatgg
//
LOCUS       DAOMVK010000303         2764 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_10821_length_2764_cov_3.260244, whole genome shotgun sequence.
ACCESSION   DAOMVK010000303 DAOMVK010000000
VERSION     DAOMVK010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10821_length_2764_cov_3.260244"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PK856_09335"
     CDS             <1..196
                     /locus_tag="PK856_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HRL02610.1"
                     /translation="QQLDSNIRGIEIKLRPVNQPKPKQIQRQFSTEALNSFDAAAEQV
                     AHHQELAETLQRFASRRRKP"
     gene            234..2252
                     /locus_tag="PK856_09340"
     CDS             234..2252
                     /locus_tag="PK856_09340"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0033543 - fatty acid beta-oxidation,
                     unsaturated, even number, reductase/isomerase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HRL02611.1"
                     /translation="MQASLLGTPLQVSGHVLRNRVIMGSMHTGFEEHPEGNEHLAAFY
                     QARAKGGVALIITGGISPNVDGVVTDEGAKLTTNEEVAWHKVVTDAAHQDGAKICMQI
                     LHTGRYAMVRQPVAPSAIQAPINPARPRALSLEEVKQTVQDYIRCAQLAQQAGYDGVE
                     VMGSEGYLINQFIAKRTNQRDDEYGGNLENRHRFAQEIVAGIRAACGDDFIIIFRLSV
                     LDLVEDGSTWEDNLQLIARIEAAGASMFNTGIGWHEARIPTIATMVPRAAFKEFTGKL
                     KQASSLPVIATNRINMPEVAESILQAGLADAVCLARPMLADAEWTNKALNQQSAAINT
                     CIACNQACLDHIFVGKLTSCLVNPFACHELQLIQTPALKKKKIAVIGAGPAGMACAKT
                     AAERGHQVTLFDALSELGGQLNIAKTIPGKQEFYEAIRYFKHELEKLGVNIQLNHRVQ
                     ADDVRDFDEIVLASGIHPRQIELEGINHSKVMTYLDVLRERKAVGNKVAIIGSGGIGF
                     DVGEFLSQDGPDSALDSHLFQQEWQIDASLAHAGGLNSPEPQLHPSKRDITICQRKLG
                     VVGRTLGKTTGWIHRLTLKERGVNMLGGLEYVKVDDAGLHIKQAGEDKVLDVDHVIVC
                     AGQVPNRDLQAALEALGKPVHLIGGAEIAAELDAKRAILSGTQVALAL"
     gene            complement(2328..>2764)
                     /locus_tag="PK856_09345"
     CDS             complement(2328..>2764)
                     /locus_tag="PK856_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003822440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRL02612.1"
                     /translation="EEAIASPLLDDVSLDGAQGVLVNITTAPGCLRMSEYKEIMSVVS
                     EYAHPDAERKYGTAEDPNMAEDQLRVTIIATGLKEQAKVAPSSLKMVRSQQATGTHGA
                     EVPNVIRSGRSARALNLGAADFADQSVLDDFEIPAMIRRQAD"
BASE COUNT          745 a          703 c          662 g          654 t
ORIGIN      
        1 acaacaactc gactctaata tccgtggcat tgaaatcaaa ttgcggcctg taaaccagcc
       61 taaacctaag cagattcagc gccaattcag taccgaagcc ttaaacagct ttgatgcagc
      121 cgctgaacaa gtggcccatc accaagaatt ggccgaaact ttacagcgct ttgccagccg
      181 tcgccgcaaa ccttgatgca ttcataataa aatacggata gaaggaaaca ccgatgcaag
      241 cttctttatt gggcacgcca ttgcaagtga gcggccatgt attgcgcaac cgtgtcatca
      301 tgggctccat gcacacaggc tttgaagaac atcccgaagg caacgagcac ttggctgctt
      361 tttaccaagc ccgcgccaag ggtggcgtag ccttaatcat aaccggtggg atttcaccca
      421 atgttgatgg tgttgtcacc gatgagggtg caaagctcac caccaatgaa gaagtcgctt
      481 ggcacaaagt ggttaccgat gctgcgcatc aagacggtgc caagatttgc atgcaaatct
      541 tgcatacagg ccgctatgcc atggtgcgcc aacccgtagc cccctctgcc attcaagcgc
      601 ccatcaatcc cgccagaccg cgtgctttaa gcttggaaga ggtcaaacaa accgtacagg
      661 attacatccg ttgtgcacaa ttggcacaac aagcaggcta tgatggcgtc gaagtcatgg
      721 gcagtgaggg ctatctgatc aatcagttta ttgccaaacg caccaaccaa cgcgatgacg
      781 aatacggtgg caacttggaa aaccgccacc gctttgcgca agaaatcgtg gcaggcatac
      841 gcgccgcttg tggtgacgat ttcatcatta ttttccgctt atcggtattg gatttggtcg
      901 aagatggttc aacttgggaa gacaatctac aactgattgc ccgcattgaa gccgcaggtg
      961 cctccatgtt taacacaggc ataggctggc atgaggcccg cattcccacc atcgccacca
     1021 tggtacctcg ggcggcattc aaagaattca ctggcaaact caaacaggcc agcagcttac
     1081 ctgttattgc caccaaccgc atcaatatgc ccgaagtggc cgaatccatt ttacaagcgg
     1141 gtttggccga tgccgtgtgc ttggccagac ccatgcttgc cgatgccgaa tggaccaaca
     1201 aagccttaaa ccaacaatct gccgccatca acacctgcat tgcttgcaac caagcctgcc
     1261 tcgaccacat ttttgtcggc aaactcacat cttgcttggt taacccgttt gcctgtcacg
     1321 aactgcaatt gatacaaaca cctgctttga aaaagaaaaa aattgctgtg ataggtgcag
     1381 gtcctgcggg catggcttgt gccaaaaccg ctgctgaacg tggccatcaa gtgacattgt
     1441 ttgatgcgtt gagcgagttg ggcggacaat tgaacatcgc caaaaccata ccaggcaagc
     1501 aagagtttta cgaagccata cgttatttta aacatgagtt ggaaaagctt ggcgtaaaca
     1561 tacaattgaa tcaccgtgta caggccgatg atgtgcgtga ctttgatgaa attgtgttgg
     1621 ccagcggcat ccatccgcgc caaattgaac ttgaaggcat caaccattcc aaagtcatga
     1681 cttacttgga tgttttgcgt gagcgtaaag ctgtgggcaa taaagtagcc atcatcggtt
     1741 cgggtggtat tggttttgat gtaggtgaat ttttaagcca agatggccca gactcagccc
     1801 tggacagcca tttatttcaa caagaatggc aaattgatgc cagcttggca catgcaggcg
     1861 gcctgaacag ccctgaaccc caattacatc catccaaacg tgacatcacc atatgccaac
     1921 gcaaactagg cgtggttggg cgcactttgg gcaaaaccac aggctggata caccgcttaa
     1981 ccttgaaaga gcgcggcgtg aacatgctcg gaggcttgga atatgtcaaa gtagacgatg
     2041 caggtttaca tatcaagcaa gcaggtgaag acaaagtatt agatgtagac catgtgattg
     2101 tgtgtgctgg tcaagtgccc aatcgggatt tacaggccgc attggaagcc ttgggtaaac
     2161 ccgtgcattt gattggtggc gctgaaatcg ctgcggagtt ggatgccaaa cgtgcgattt
     2221 tatcgggtac acaagtcgct ttggcgttat aagattttgc tcactgtttc tatcaaacaa
     2281 aaaagcctgc tttaaagcag gctttttatc ataatcggtt cagcacatca gtcggcttga
     2341 cgacgtatca tggctgggat ttcaaaatca tccaataccg attgatcggc aaaatcagct
     2401 gcgcccaaat tcaaagcacg cgcactgcgg cctgaacgaa tcacattagg cacttccgca
     2461 ccatgagtgc ctgttgcttg ttgagagcgc accattttca atgaagatgg ggccactttg
     2521 gcttgctctt tcaaacctgt tgcaatgatg gtcacacgca attgatcttc agccatgtta
     2581 gggtcttcag cggtaccgta tttgcgctca gcatctggat gggcatattc actcacgaca
     2641 gacatgattt ctttgtactc agacatgcgc aagcaaccag gagccgtggt gatgttcacc
     2701 aacacacctt gcgcgccatc caaagacaca tcatccaaca atgggctggc aatcgcttct
     2761 tctg
//
LOCUS       DAOMVK010000304         2763 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_10832_length_2763_cov_2.378139, whole genome shotgun sequence.
ACCESSION   DAOMVK010000304 DAOMVK010000000
VERSION     DAOMVK010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10832_length_2763_cov_2.378139"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PK856_09350"
     CDS             complement(<1..376)
                     /locus_tag="PK856_09350"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRL02613.1"
                     /translation="MEWGMVVAFWGVSMLFIMSPGADWSYAISAGIHKKVVPSVLGML
                     SGHVLATLVVAAGVGVVLAQHPQALMALSLLGAAYLAWMGWQMFRQPATPSAGEQMDD
                     THWGKWCWKGLCVSGLNPKVLLL"
     gene            497..949
                     /locus_tag="PK856_09355"
     CDS             497..949
                     /locus_tag="PK856_09355"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRL02614.1"
                     /translation="MNKIDKKILAHLQAEGRLSVTELAERVGLSVSPCHRRVKVLEDT
                     GVIQGYRAQLNPELVGLNFSAIIFVTLKDGNRDSLHAFEAAIIDIPQIVQAQRLFGDP
                     DYLLQIMTTNLASFQKLYDEKLSAIPSIQRLTSTIVMKDVVSGRALPI"
     gene            complement(1034..2326)
                     /locus_tag="PK856_09360"
     CDS             complement(1034..2326)
                     /locus_tag="PK856_09360"
                     /inference="COORDINATES: protein motif:HMM:NF007710.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS family transporter"
                     /protein_id="HRL02615.1"
                     /translation="MEQTQLNSTGARIKAIIGSASGNLVEWYDFYIYSFCALYFAHAF
                     FPKGDQTTELLQAAAVFAVGFFMRPLGSWYFGRLADRVGRKHSMLISVLMMCAGSLVI
                     AFVPTYETIGMAAPIILLLARITQGFSVGGEYGATATYMSEVATSHNRGFLSSFQYVT
                     LIGGQLLASAVVTVLLLVLSKEQMSDWGWRIPFFIGALLAVVSIIMRRNLIETISKKH
                     TENKEAGSMKALWKNPRAMLIVLGITAGSSTIFYTFTTYMQKFLVNTSGFEKDTATFI
                     MTFVLLIFMVAQPVYGMISDKIGRKLCMVLFGGFGVLTTVPLMSSIAQTNNVTIAIVL
                     ILLGLAWISFYTSISGIVKADLFPAHVRALGVGLPYAIANAIFGGSSEFLALKLKSVG
                     HEEYFFYYVSVVMAIALIAALAMPDNRKHSLIDKDTHL"
BASE COUNT          809 a          711 c          568 g          675 t
ORIGIN      
        1 acaacagcaa aaccttcgga ttcaagccgc tcacacacaa tcctttccaa caccacttgc
       61 cccaatgcgt gtcatccatt tgttcgcccg cactcggtgt cgcaggttgg cgaaacattt
      121 gccagcccat ccaagccaaa taagccgcgc ccaacaggct caaggccatc aatgcttgcg
      181 gatgctgtgc caacaccaca cccactcccg ctgccaccac caacgtggcc aatacatgcc
      241 cactgagcat gcccaatacc gatggcacca cttttttatg tatgcccgcg ctgatggcat
      301 acgaccaatc cgccccaggg ctcatgatga acaacatcga cacaccccaa aatgccacta
      361 ccatgcccca ttccatttgc ttccttgcct catccgtgtg gatgtgttga ttgatgtggc
      421 aatcttagcc aatagtcctt agaatgggct gtcaaagttt tgtgcaaatt ttaagcaaat
      481 tggaaaattt ttctacatga acaagataga taagaaaatt cttgcgcatc tacaagccga
      541 agggcgatta tctgtcaccg aattggccga gcgcgtcggt ttgagcgtgt cgccttgcca
      601 ccgccgcgtc aaggttttgg aagacacagg cgtgatacaa ggctaccgcg cgcaattgaa
      661 tcccgaattg gtgggactga atttttccgc aattattttt gtgaccttaa aagacggcaa
      721 ccgcgattct ttgcacgctt ttgaagccgc catcatcgac attccgcaaa tcgtgcaagc
      781 gcaacgcttg tttggcgacc ccgattattt gttgcaaatc atgaccacca atttggccag
      841 ttttcaaaag ctttacgatg aaaaactctc cgccataccg agcatacagc gcctcacctc
      901 caccatcgtg atgaaagacg tggtgtcagg ccgtgccttg ccgatttaaa ccaatgttta
      961 cttgaatcag atacaagcaa gcggcctgta agttcaatac aaacttacag gccgcttatt
     1021 tatggtgcga atcttacaaa tgggtgtcct tgtctatcaa actgtgtttg cggttgtcag
     1081 gcatggccaa tgcagcaatc aaggctatgg ccatcaccac cgacacataa tagaaaaaat
     1141 actcctcatg ccccaccgat ttcagtttca aggccaagaa ttctgagcta ccaccaaaaa
     1201 tcgcattggc aatggcataa ggcaagccca cgcccaaggc gcgtacatgg gctggaaaca
     1261 aatctgcttt cacaatgcca ctgatggagg tataaaaact gatccaagcc aaacccagca
     1321 aaatcagcac gatggcaatg gtgacattat tggtttgagc gatgctgctc atcaaaggca
     1381 cggtggttaa cacaccaaag ccaccaaaca agaccatgca cagcttgcgg cctatcttat
     1441 cggaaatcat cccatagacg ggttgggcca ccatgaaaat caacagcaca aacgtcatga
     1501 tgaaggtggc tgtgtctttc tcaaacccag aggtgttgac caaaaacttt tgcatatagg
     1561 ttgtgaaggt ataaaaaatg gtggagctgc ctgcggtgat gcctaacact atcagcatcg
     1621 cacgtggatt tttccataaa gccttcatgg agccagcttc tttgttttcc gtgtgtttct
     1681 tgctgatggt ttcaatcaga ttgcgacgca tgatgatgga cactaccgcc aacagcgcac
     1741 caatgaaaaa ggggatgcgc cagccccagt cactcatttg ctccttactc aacaccaaca
     1801 acaataccgt gaccactgca gacgccaaca attgcccacc aatcaaagtc acatactgaa
     1861 aggaagacaa gaagccccga ttgtgtgaag tagccacttc gctcatgtaa gtggcggtgg
     1921 cgccatactc accacccacc gaaaaacctt gggtgatgcg tgccaacagc aaaatgatag
     1981 gcgcagccat gcctatggtt tcgtaagtgg gcacaaaggc gatgaccaaa gagcctgcac
     2041 acatcatcaa cacggaaatc aacatggagt gtttgcggcc tactcggtcg gccaaacgtc
     2101 caaaatacca gcttcccaat ggtcgcataa aaaagccgac tgcaaacacg gcagcggcct
     2161 gtaacaattc ggtggtttgg tcgcctttag gaaaaaaggc atgagcaaaa tacagcgcac
     2221 aaaacgagta aatgtagaaa tcataccatt caaccaaatt gcctgatgcg ctgccgatga
     2281 tggctttgat gcgtgcacca gttgagttca attgtgtttg ttccattgtc tctccttgtt
     2341 tttggttttt gttctaaact cggatttgca atataaaaga tatttttttg gcatgctagc
     2401 aaaaaaatac cttacactgc gatacacaaa tttattcaat tttgaataaa aaacaccacg
     2461 cataaacagc tgaaaatact gaatttaaac caaaaaatac ccaaaataaa tgctgggaaa
     2521 attttttatt tctgtgatga ataatcccaa ttttttcact tttccaaaat aaaaatacag
     2581 actgtttcaa ttcggtattt tgtttgttgc ataaaaaagc cacctgaact caggtggctt
     2641 gatagataaa cgctcatact catttcatta cactattgca ttaatgaatc gttttgctga
     2701 tgccttgatt caccacgcca ataggcaacg tcaccaaatc cgccgccaaa gtgaatggcg
     2761 ttg
//
LOCUS       DAOMVK010000305         2760 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_10854_length_2760_cov_1.921627, whole genome shotgun sequence.
ACCESSION   DAOMVK010000305 DAOMVK010000000
VERSION     DAOMVK010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10854_length_2760_cov_1.921627"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1634
                     /locus_tag="PK856_09365"
     CDS             <1..1634
                     /locus_tag="PK856_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRL02616.1"
                     /translation="QSQPDVNNGAQATQNNSALVASAPVAMPSRTEVPSIIPTATPQR
                     LASLDASSVVLPNVQLLQAPVDDANLRPDDELLINNGIIIEEKLAEFKVNVSVTDAYA
                     GPVITRYEIEPATGVRGSQVVNLEKDLARSLGFASIRVVETIPGKTCMGLELPNPKRQ
                     MIRLSSVFQAPVFQESKSKLTMALGQDISGNPVVTDLAKAPHLLVAGTTGSGKSVGVN
                     AMILSMLFKATPEEVRMIMIDPKMLELSIYEGIPHLLAPVVTDMKLAANALNWCVNEM
                     EKRYRLMSHMGVRNLAGYNQKIADAKARGQTIVNPFSITPDEPDLLEKLPFIVVVVDE
                     FADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANIPTRIAFQV
                     SSKIDSRTILDQMGAENLLGQGDMLFLPPGTGYPRRIHGAFVADDEVHNVVEYLKQFG
                     EPDYVDEILSHGGLDDSSSGASGNGGGEEDPLYDQAVKYVISSGKTSISAVQRQMRIG
                     YNRAARLVDQMEAAGVLSAPEHNGNRSLLVPQREM"
     gene            1726..>2760
                     /locus_tag="PK856_09370"
     CDS             1726..>2760
                     /locus_tag="PK856_09370"
                     /inference="COORDINATES: protein motif:HMM:NF021375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HRL02617.1"
                     /translation="MQRCYYHNSSSGFVSEPQYEILGKLTQNHGFDLNDLQRNAWLAQ
                     INVLKKQLHNIPVNQVFFEFAIPRMGRRVDNIVLLPNAILVLEFKVGATHYTQQAKNQ
                     TIDYCLDLLNFHAESHGKKVIPVLVCTQAPAMTVQWALVDQLQECVCCNQDNLNETLL
                     SLVQQCADAPLIDADKWQQSAYKPTPTIIEAAQALYKQHQVGEITRHDAGATNLAVTS
                     ARINHIIEDAKQQNNKVICFLTGVPGAGKTLAGLNIANERLHADENEHAVFLSGNGPL
                     VSVLREALVRDAVAIAKEQGLKLSKNTVQPKTQAFIQNIHHFRDEYVDTDAVPLEKVV
                     VFDEAQRAWDK"
BASE COUNT          741 a          641 c          703 g          675 t
ORIGIN      
        1 aacagagcca gcccgacgtg aataatggtg ctcaggctac acaaaacaac tctgcactgg
       61 tcgcatcagc tcctgttgcc atgccgagta gaacagaagt gccttccatc atcccaaccg
      121 caactcctca gcgtttggca agcttagacg catcaagcgt ggtgttaccg aatgtgcaat
      181 tgctgcaagc acctgtagat gatgcgaatt tgcggccgga tgatgaattg ctgattaaca
      241 atggcatcat catcgaggag aaactggcag aatttaaggt caacgtcagc gtgaccgatg
      301 cttatgctgg ccccgtgatt acccgctatg aaatcgagcc tgccacgggc gtgcgcggtt
      361 cacaagtggt gaatttggaa aaagacttgg cgcgctcatt gggctttgcc tctatacgag
      421 tggtggaaac cattcctggt aaaacgtgta tgggtttgga attgcccaac cccaaacgcc
      481 aaatgatacg tctgtcatcg gtatttcaag caccggtgtt tcaagagtcc aaatcaaaat
      541 taaccatggc cttgggtcaa gacatttcag gcaatcccgt ggtcaccgac ttggccaaag
      601 cccctcattt gttggtggca ggtaccacag gctcaggcaa atccgtgggc gtgaatgcga
      661 tgattttgtc catgctcttc aaggccacgc ctgaagaagt gcgcatgatc atgattgatc
      721 cgaaaatgct ggagttgtcg atatacgaag gcattccaca tttgctcgcg cctgttgtga
      781 ccgacatgaa gcttgcggcc aatgcgctca attggtgtgt gaacgaaatg gagaagcgtt
      841 atcgcttgat gagccacatg ggtgtgcgca atttggcggg ttacaaccaa aaaattgccg
      901 atgccaaagc gcgtggccaa accattgtta acccattcag catcacgcct gatgaacctg
      961 atttgttgga aaaactgccc tttatcgtgg tggtggtgga cgagtttgcc gacttgatga
     1021 tgacggcagg caaaaaaatc gaagaattga ttgcgcgctt ggcccaaaaa gcccgtgcag
     1081 caggcataca tttgattttg gcaacccaac gcccatcggt agatgtgatt actggtttga
     1141 ttaaggccaa tattcctacc cgtatcgcct tccaagtgtc gagcaaaatc gattcacgca
     1201 ccatcctcga ccaaatgggc gctgaaaacc tgttaggcca aggcgatatg ctgttcttgc
     1261 cgccaggcac aggctacccg cgccgcattc atggcgcgtt tgtggccgat gatgaggtgc
     1321 acaacgtggt ggagtatttg aaacaattcg gcgaacccga ttatgtggat gaaattctca
     1381 gccatggcgg cttggacgac agcagcagtg gtgcgagcgg caatgggggt ggcgaagaag
     1441 accccttgta cgatcaagcc gtgaaatacg tgatttccag cggcaaaacc tctatctcag
     1501 ctgtacaacg ccaaatgcgc ataggctata accgcgccgc ccgtttggtc gaccaaatgg
     1561 aagctgcagg cgtgttgtca gcccccgaac acaacggcaa ccgcagcttg ctggtaccac
     1621 aacgcgagat gtgattttgc agtgttttaa aacttacagg ccgcttaatg cggcctgtaa
     1681 cattattttg gtatgcaata gatgtattga ttgggagcgg ctgtgatgca acggtgttat
     1741 taccacaatt cgagttcagg ttttgtgtct gagccacagt atgaaatttt ggggaaatta
     1801 acccaaaatc atggttttga tttgaatgat ttgcagcgca acgcttggtt ggcacaaatc
     1861 aatgttttaa aaaaacaatt acacaatatt cccgtaaacc aagtgttttt tgaatttgcc
     1921 attccccgca tgggcaggcg tgtagacaat atcgtgttat tacccaatgc cattttggtg
     1981 ttggagttca aagtgggtgc cacacattac acgcaacagg ctaaaaatca aaccattgat
     2041 tactgcttgg atttgttgaa ttttcatgca gaaagccacg gtaaaaaagt gattcctgtg
     2101 ttggtgtgca cgcaagcacc tgcaatgaca gtgcaatggg cgttggttga tcaattacaa
     2161 gagtgcgtgt gctgtaatca agacaatcta aatgaaacac tgttatcgct ggtgcagcaa
     2221 tgtgctgatg caccccttat tgatgcagac aaatggcagc agtctgctta caaaccaacg
     2281 cctaccatca tcgaagccgc tcaagctttg tacaagcaac accaagtggg tgaaatcacc
     2341 cgccatgatg ccggtgccac caatttggca gtgacttcgg cccgcatcaa tcacattatt
     2401 gaagatgcca agcaacaaaa caacaaagtg atttgttttt tgaccggagt gcctggcgca
     2461 ggcaaaacct tggcgggctt gaatattgcc aatgaaagat tgcatgctga tgagaatgaa
     2521 catgcggttt ttttgtctgg caatgggcca ctggtttccg tattaagaga ggctttggtc
     2581 agagatgcgg tggcgattgc gaaagagcaa ggcctcaagc tcagtaaaaa cactgtacag
     2641 cccaaaaccc aggcatttat tcaaaatatc caccactttc gcgatgagta tgtcgacaca
     2701 gatgccgtgc cattggaaaa agtggtggtg tttgatgagg cccaacgggc ttgggacaaa
//
LOCUS       DAOMVK010000306         2742 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_10945_length_2742_cov_3.471530, whole genome shotgun sequence.
ACCESSION   DAOMVK010000306 DAOMVK010000000
VERSION     DAOMVK010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10945_length_2742_cov_3.471530"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..589
                     /locus_tag="PK856_09375"
     CDS             110..589
                     /locus_tag="PK856_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02618.1"
                     /translation="MEMLPINDFNSGVTMWMINVFLRVLFINVWLIVLAILILKIPAL
                     NSSFNIALLIVLTVYHGTVLSFYALSLNYSVYQQKIDSKFLEQTRTALYFMPVFIVLY
                     TFNFQGVQLNNQQDLTLKSVSGTLLNKYQTPSLISKLECLKSDCLYLKIAEDEYGGI"
     gene            576..866
                     /locus_tag="PK856_09380"
     CDS             576..866
                     /locus_tag="PK856_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02619.1"
                     /translation="MGVFECAVYDNEGCTFINREYFYQSKANHLTDVRIDFLQDTNLA
                     YALQIGNTIKYPRAYFEEKYAQEKQDAKLAIGILLALFLLTPLYLKKMFLLI"
     gene            1106..1291
                     /locus_tag="PK856_09385"
     CDS             1106..1291
                     /locus_tag="PK856_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3079 domain-containing protein"
                     /protein_id="HRL02620.1"
                     /translation="MRFPKQPKFPERLCWGCNKLCSVSDLQCGNGQDRAQHPIEIFGA
                     DWYVDLTPEELDKIDLV"
     assembly_gap    1503..1514
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1515..1843)
                     /locus_tag="PK856_09390"
     CDS             complement(<1515..1843)
                     /locus_tag="PK856_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRL02621.1"
                     /translation="MSEPHYTYLHTLADLDAHQDWLPEIFAIHKQLRPAMNDFAAYEQ
                     LLRTMMSENQRLLLVDYGKPPFIGIAMFNMHHNTYQNKMMMLEDLVIDESLRGTNIGS
                     QVLTEIER"
     gene            complement(1843..2361)
                     /locus_tag="PK856_09395"
     CDS             complement(1843..2361)
                     /locus_tag="PK856_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HRL02622.1"
                     /translation="MHQVRIKQVQADITSLAVDAIVNAANKSLLGGGGVDGAIHRAAG
                     PELLSACIALRGCNTGEAKMTSGYLLPALHVIHTVGPVWHGGQHGEAELLAACYRHAL
                     ELADVHQIRSIAFPCIATGIYAYPPEQAAQIAVDTVRDIAPTLSHVEEIIFCCFHTED
                     AALYQSLLKTEN"
     gene            complement(2365..2694)
                     /gene="cyaY"
                     /locus_tag="PK856_09400"
     CDS             complement(2365..2694)
                     /gene="cyaY"
                     /locus_tag="PK856_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003787772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HRL02623.1"
                     /translation="MNETEFVTLSENIFDHIDDYVSDHFDAFDTEKSGNVLTLEHEDG
                     AQIIINRHAANQELWIAAKSGGYHFQYKNGAWVASRDGSEFYAVLSQTLSDLLGENVT
                     VPALQAD"
BASE COUNT          681 a          576 c          693 g          780 t
ORIGIN      
        1 gaccaattgt aaggcgattt gggtgtttta aagcggcctg taaacccatt gaaaaaggtt
       61 ttcaggccgc ttaagtcttg gcttgtgtat tgctactggc aatcatgcaa tggagatgct
      121 ccccatcaac gattttaaca gcggtgtaac catgtggatg attaatgtgt ttttaagagt
      181 gctattcatc aatgtgtggc tcatcgtttt ggccatactc attctgaaaa ttccagctct
      241 aaactccagt tttaacattg ccctgctcat tgtattgact gtttatcatg gcaccgtact
      301 ttctttttat gcgttaagct tgaactattc cgtgtatcag caaaaaatag acagtaaatt
      361 tcttgagcaa accagaacgg cattgtattt tatgcctgta ttcattgttc tctatacctt
      421 caattttcag ggcgtacaat taaacaatca gcaagacctc acgcttaaga gtgtttctgg
      481 gacgctatta aataagtatc aaactcctag tttaatatct aaacttgagt gcttgaaaag
      541 cgattgtttg tatctcaaaa ttgctgaaga tgaatatggg ggtatttgaa tgtgctgttt
      601 atgataatga gggctgcact tttatcaacc gtgaatattt ctatcagtcc aaagcgaatc
      661 atctgactga tgtccgaatt gatttcttac aagacaccaa cttggcttat gcattacaga
      721 taggtaatac catcaaatac cctcgagcct attttgaaga aaagtacgca caagaaaaac
      781 aagatgccaa gctggccatt ggtattttgt tggcactgtt tcttttaaca cctttatacc
      841 ttaagaagat gtttctctta atttgatgcg cttagctacg gcaagcgttg acacggtttg
      901 ggttatattt agacgattct gtatggcagg taacaagcgg cctgtaaacc cacaaaaaag
      961 gtttacaggc cgcttgtatt tgcttgcgtt gcatcaataa gcccgatgga taaaggagta
     1021 agctaaaccc atactcaatg cgataagccc tgtatcaaac agggttttgg ataggaggag
     1081 aatggcaagt gtccgtgggc agaaaatgcg ttttcccaag cagccgaagt ttccagagcg
     1141 tttgtgctgg ggttgcaaca agttgtgttc ggtgagcgat ttgcaatgtg gcaatggcca
     1201 agaccgtgcg cagcatccga tagagatttt tggcgcagat tggtatgtcg atttgacgcc
     1261 tgaggagttg gataaaattg atttggttta agcaaactca gatgtgatac tttcattcct
     1321 ttaaagcaaa aaagcggcct gtaatatagt tacaggccgc tttttgatgc tgagtgacac
     1381 atcacaaggc tttggtaaag tggaagcttt cggccacata gccgtggagg aaataaaatt
     1441 tgtgtgcgcg gccacggtac acgcccgaat ccaaacccaa gtgctcacaa ccgttttcgc
     1501 ggnnnnnnnn nnnncgctca atttcggtta acacttggct gccaatgttg gtgccgcgca
     1561 gggattcgtc tatgaccaag tcttctaaca tcatcatttt gttttggtag gtgttgtggt
     1621 gcatattgaa catggcgatg ccgatgaagg gcggcttgcc ataatccacc aataagaggc
     1681 gttggttctc gctcatcatg gtgcgcagca attgctcgta agccgcgaaa tcattcatgg
     1741 ctgggcgcaa ttgtttgtgg atggcaaaga tttcaggcag ccaatcttgg tgtgcatcca
     1801 agtcggccag tgtgtgcaaa taggtatagt gtggctctga catcagttct ccgttttcaa
     1861 tagactttgg tacaaggctg cgtcttctgt gtggaagcag caaaaaatga tttcttcgac
     1921 atggcttaag gtgggggcga tgtcgcgcac ggtatccaca gcaatctgcg ctgcttgttc
     1981 gggtggatag gcgtaaatgc ctgtggcaat acaggggaaa gcgatgctgc gtatttggtg
     2041 cacatcggcc aattcaagtg cgtggcggta gcaggcggcg agcagctcag cctcgccatg
     2101 ctggccgccg tgccaaacag ggccaacggt gtggatgaca tggagggcgg gcaataaata
     2161 acccgaagtc attttggcct cgcctgtgtt gcagccgcgc aaggcgatgc aagccgacag
     2221 caattctggc ccagcggcac ggtgtatggc gccatccacg ccgccaccgc cgagcaggct
     2281 tttgttggca gcgttgacga tggcatctac ggccaaggaa gtgatgtcgg cttgaacttg
     2341 cttgatgcgc acttgatgca tgacttaatc ggcctgtaaa gcaggtacgg tcacgttttc
     2401 acccaataag tcgcttaagg tttggctcaa tacggcataa aattcgctgc catcgcggct
     2461 ggccacccaa gcgccgtttt tgtattgaaa gtggtagccg ccgcttttgg ctgcgatcca
     2521 caattcttgg ttggcggcgt ggcggttgat gatgatttgt gcgccgtctt cgtgttcgag
     2581 ggtcaacaca ttgcctgatt tttcggtatc gaaggcatcg aaatggtcgc tgacgtaatc
     2641 gtcgatgtgg tcaaaaatgt tttctgataa ggtgacaaac tcggtttcat tcatgctcat
     2701 ggtgtctgac ggtgtatttt gttaagattg cccatcttgc ca
//
LOCUS       DAOMVK010000307         2732 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_11008_length_2732_cov_3.092641, whole genome shotgun sequence.
ACCESSION   DAOMVK010000307 DAOMVK010000000
VERSION     DAOMVK010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11008_length_2732_cov_3.092641"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..831
                     /locus_tag="PK856_09405"
     CDS             91..831
                     /locus_tag="PK856_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HRL02624.1"
                     /translation="MKHQNMGLTFTKATQPSTAQNRVGKIGLLHEQGTQVDFSQWLLF
                     VVSTFLISATPGPNMLLAFQYGLNYGFKKTMWTLAGLTCGLTIWLVLSISGVAVLSQK
                     VPVAFEVFKCLGALYLAYLGWQAWQSKGSDLRSDVEPVVPTPAKLFRMGVVVALSNPK
                     AILFFAAFFPKFVDASLPQAPQYLVLTVSFFVIETVWQLIYTVSGKALADWLQQGRRL
                     QILNRVCGAIFILIAVSLLWDSMHVLLG"
     gene            complement(951..1412)
                     /locus_tag="PK856_09410"
     CDS             complement(951..1412)
                     /locus_tag="PK856_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02625.1"
                     /translation="MKQTVATLLRVLGILLGLAWIWGSLANIVFPDVYVCNTGNNCPS
                     PPSVLERAAANALFFVTGCLLLVPQQKCRQPIVRKVYGLMLAGFNLFLAAFWLWQLGP
                     LLFGDFITSSQITNSVVIFGCLFITLGNLWAFYSMLKQEAQSMPSDSIHAD"
     gene            complement(1511..>2732)
                     /locus_tag="PK856_09415"
     CDS             complement(1511..>2732)
                     /locus_tag="PK856_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HRL02626.1"
                     /translation="TDHHLPADTLPACTIVNPNQPDCGFGSKNLAGCGVMFYVLLALR
                     AHYKLTGRYDSQAAPNLGVLLDYVALGTVADVVKLDHNNRILVAQGLKRMRAGKAALG
                     IQALFEIAKRDIRKAEPFDLGFAIGPRINAAGRLDDMSIGIRCLLADNELTAQTLAAE
                     LDSLNRERRGIEQSMLKEALNLPEFQVSDSQMTVTAYRDDWHQGVIGIVAGRLRERYF
                     RPTIVFAPGDDGELRGSGRSIPALHLRDTLDLVSKRHPHLISKFGGHAMAAGLSLPEA
                     ALPEFQAAFEQAVNEQLHRDDLTQTYVTDGELSGDAMCLQAAQQLREVVWGQGFAPPM
                     FVGEFDVTWQRAVGVNHKKAGIRKTGNAEVEAMFFRCEEDLPERIRMVYRMVANEWRG
                     QYELQLHSEYWEAV"
BASE COUNT          644 a          680 c          747 g          661 t
ORIGIN      
        1 gttaaggcaa agtgcgtgtt ttttttcatg gtggccaatt gtaactgaga atggttttta
       61 ttaacaagtg atttgatgca cgatgtcacc atgaaacacc aaaatatggg tctgactttc
      121 accaaggcaa cacagccatc gacagcacaa aacagagtcg gcaaaatagg gcttctgcat
      181 gaacaaggga cgcaagtgga cttttcgcaa tggttgttgt ttgtggtgag cacgtttttg
      241 atttcggcca caccaggccc aaacatgttg ctggcatttc aatacggttt gaattacggt
      301 tttaagaaaa ccatgtggac gctggcgggt ttgacttgcg gcttgaccat ttggctggtg
      361 ttgtcgattt ctggggtggc ggtactcagc cagaaagtgc ctgtggcgtt tgaggtgttc
      421 aaatgtttgg gggcgctgta tttggcttat ttgggctggc aagcgtggca aagcaagggc
      481 agcgacttgc gcagcgatgt cgagcctgtg gtgcccacgc cagctaaatt gtttcgcatg
      541 ggcgtggtgg tggccttgtc caatcccaag gccattttgt tttttgccgc tttctttccc
      601 aaatttgtcg atgcaagctt gccgcaagca ccacaatatc tggtgttgac ggtgtcgttt
      661 ttcgtcatcg aaacagtgtg gcaattgatt tacaccgtca gcggcaaggc cttggcggat
      721 tggttgcaac aaggccgccg tttgcaaata ttgaatcggg tctgcggcgc catctttatc
      781 ttgattgccg tgagtctgtt gtgggacagc atgcacgtct tattggggta agctgagtag
      841 gaaacaaaca agcggcctgt aaatcagtta caggccgctt gtttgcgata aattgggtga
      901 tgaggtttgc cagtcaaaac agtgtgttta aaactgtgca gcgcgcacag ttaatcagca
      961 tggatggaat cgctaggcat ggactgcgcc tcttgtttca acatcgaata aaacgcccac
     1021 aaattgccca aagtgatgaa gagacagcca aaaatcacca cactgttggt gatttgcgat
     1081 gaagtaataa aatcaccaaa cagcaatggg cccaattgcc acagccaaaa tgccgccaaa
     1141 aacagattga aacccgcgag catcaaacca tacactttgc gaacaatcgg ctgtcgacac
     1201 ttttgctgcg gcacgagcaa taagcaacct gtcacgaaaa acagcgcatt ggccgcggct
     1261 ctttccaaca cacttggagg cgatgggcaa ttattgcctg tgttgcagac ataaacatca
     1321 gggaaaacaa tgttggcaag cgagccccaa atccaagcca gccccaaaag tatgcccaac
     1381 acccgcagca gggtggcgac agtctgtttc atctgcttgg cgtcttccaa gtgatttcaa
     1441 taggtatggc tgtgtgtgag tttacaggcc gtttgtcatg ctcttattca atcgcctgag
     1501 cgttttgggc ttacaccgct tcccagtatt cactgtgcaa ttgcaattcg tattggccgc
     1561 gccactcgtt ggccaccatg cggtaaacca tgcggatgcg ttcgggtaaa tcctcttcgc
     1621 agcggaaaaa catcgcttcg acttcggcat tgcctgtttt gcgtatgccg gcttttttgt
     1681 ggttcacacc gacggcgcgt tgccacgtta cgtcaaattc gccgacaaac atcggcggcg
     1741 caaagccttg tccccacacc acctcacgca attgttgggc ggcctgtaaa cacatggcat
     1801 cgccagacag ctcgccatcg gtgacatagg tttgggtcaa atcatcgcgg tgcaattgtt
     1861 cgtttaccgc ctgctcaaat gcggcctgaa actcaggcaa agccgcttcg ggcaaactta
     1921 agcctgcggc catcgcatgg ccaccgaatt tgctgatgag atgcgggtgg cgtttggaga
     1981 ccaaatccaa ggtatcgcgc aaatgcaagg caggaatcga acggccagag ccgcgcaatt
     2041 cgccatcgtc acctggggcg aacacaatgg tggggcggaa atagcgttca cgcaaacgac
     2101 ctgccacgat gccgatgacg ccttgatgcc agtcatcgcg gtaggcggtg accgtcattt
     2161 ggctgtcgct cacttggaat tcgggcaggt ttaaggcttc tttgagcatg gattgctcaa
     2221 tgccacggcg ttcgcggttg aggctgtcga gttcagccgc gagggtttgt gccgtgagct
     2281 cattgtcggc gagcaaacag cgtatgccta tgctcatgtc gtctaagcgg cccgcggcgt
     2341 tgatgcgagg gccgatggca aagcccaaat caaacggctc agccttgcga atgtcgcgtt
     2401 tggcaatctc aaacaaagct tgtatgccca aagcggcttt gcctgcgcgc atgcgtttga
     2461 ggccttgtgc caccaaaatg cggttgttgt ggtctagctt caccacatcg gccaccgtgc
     2521 ccaaggcgac gtaatccaat aacacgccca aattgggcgc ggcttgcgag tcatagcggc
     2581 ctgtaagctt gtaatgcgcg cgcaaagcca gcaacacata aaacatcacc ccacagcctg
     2641 cgaggttttt gctgccaaac ccacagtcgg gctgattggg gttgacgatg gtgcaagcgg
     2701 gcaaggtatc ggcgggcaaa tggtggtcgg tg
//
LOCUS       DAOMVK010000308         2726 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_11046_length_2726_cov_3.062149, whole genome shotgun sequence.
ACCESSION   DAOMVK010000308 DAOMVK010000000
VERSION     DAOMVK010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11046_length_2726_cov_3.062149"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..1557
                     /locus_tag="PK856_09420"
     CDS             97..1557
                     /locus_tag="PK856_09420"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL02627.1"
                     /translation="MKSTLALSSLFTAVALLFTGVGLFIFFSNIELTNAGISSEKIGF
                     INAGFYLGALLSGIIAQRWVAKVGHIRAFALFTAIITIGILLHVVVKQEIVLIVLRVI
                     LGFSDYSLLMVVESWLNARSTNQNRSSILAVYTMIFYMAFMLASLVIATPIESATMYV
                     LCAILAAASIIPIMLTRIEQPQLPQKMGISFPKLWEVVPLALLGSVTAGFLVGGFITM
                     APVYVALRGLDNQVASHFILAAMCAGLVIQIPMGKFSYAFGRRSGIILACSFALTAAI
                     LMWLLGQYAWVLVASAFIMGCGVFTMYSLSSARANDSIPPHSNVVEIGRSMLFSYCIG
                     SFLSPLILGFLMKYMGAHGFTLQYVVLAGMLLLFALTQAVIPRTKRGPYVSMPVNAGV
                     GSVLLDQSLTQEEATDKDAQEVKTSVEHESHETAEQSEEDLQAASEASSELKMNESNK
                     EDLTITDNADANVDEVFSPVQQDLAIKEAPRPPAET"
     gene            1649..>2726
                     /gene="dnaJ"
                     /locus_tag="PK856_09425"
     CDS             1649..>2726
                     /gene="dnaJ"
                     /locus_tag="PK856_09425"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HRL02628.1"
                     /translation="MSQRDFYEVLGVSKGASEAEIKKAYRKLAMKYHPDRNPDDKEAE
                     EKFKEIQKAYDILSTPEKKAAYDQYGHAGVDPNMGGGGGFGGGFGGADFDINDIFSQM
                     FGGAGGGGRSRQPNYQGADLRYDLAISLEEAAHGVKKKITFATFEECDVCHGSGAKAG
                     SEPVTCHTCHGSGTVHIRQAIFQVQQTCPTCQGSGVEIKDPCVKCKGHGKVRSSRTLE
                     VNVPAGVDTGSRIRLSGEGEAGTHGAPSGDLYVFIEVKEHNIFQRDGMDLHCEVPIGF
                     TTAALGGDVEVPTIDGRVILKIAPETQFGRKMRIKGKGIKSLRSSSVGDLYCHIVVET
                     PVNLTERQKELLEEFETISTGQERS"
BASE COUNT          669 a          625 c          695 g          737 t
ORIGIN      
        1 agtgatgggg ttttttctct atttctgtga taatccagct tgcttttcag cacattctaa
       61 acgccaaatg tacccaaatg atattggaat taactcatga aatccactct ggctttgtct
      121 tcgctattca ccgctgttgc attgttgttt acaggtgtgg gcctgtttat ctttttttcc
      181 aatattgagc tcaccaatgc gggcatcagc tctgaaaaaa ttggtttcat caatgcgggt
      241 ttttatttgg gtgctttact cagtggcatc atagcacaac gctgggtggc caaagtaggc
      301 catatccgtg catttgcctt gtttaccgcc attattacca taggcatttt attgcatgtg
      361 gtggtgaaac aagagattgt tttgatagtg ttgcgggtga ttttgggttt cagtgattac
      421 agcctgttga tggtggtgga aagttggctc aatgcacgca gcaccaacca aaaccgcagc
      481 agtattttgg cggtttacac catgattttc tacatggcct ttatgttggc gtctttggtg
      541 attgccacgc cgatagaaag cgccaccatg tatgtgttgt gtgccatttt ggccgccgca
      601 tccatcattc ctatcatgtt aacccgcatt gagcagccgc aattgccgca aaaaatgggc
      661 atcagttttc ccaagttgtg ggaagtggtg cctttggcct tattaggcag tgttaccgca
      721 ggctttttgg tgggcggctt tatcaccatg gcgcctgtgt acgtggcctt gcgaggtttg
      781 gacaaccaag tggcttcgca ctttatcttg gctgccatgt gtgcaggctt ggtcatacaa
      841 atcccgatgg gtaaattctc ttacgcgttc ggtcgtcgca gtggcatcat tttggcctgt
      901 tcgtttgcct tgactgccgc cattttgatg tggctgctgg ggcaatatgc ttgggtattg
      961 gtcgcttctg cattcatcat gggttgcggc gtattcacca tgtattcttt gagttctgcc
     1021 cgtgccaacg acagcattcc accgcattcc aatgtggtgg aaattggtcg cagcatgttg
     1081 ttcagctatt gcataggttc gtttttgtcg cccttgattt tgggtttcct catgaaatac
     1141 atgggtgcac acggttttac cctgcaatac gtggtgttgg cgggcatgtt gttgttgttt
     1201 gctttgaccc aggccgtgat tccccgcacc aagcgtggcc catatgtgtc catgcctgtc
     1261 aatgcaggcg tcggcagtgt tttgcttgat cagagcttga ctcaagagga agcgacagat
     1321 aaagacgctc aggaagtcaa aacaagtgta gaacatgaat cacatgaaac agcagaacaa
     1381 tctgaagagg atttacaggc cgcatcagaa gcatcaagtg agttgaaaat gaatgagtcg
     1441 aacaaggaag atttgaccat aacagacaat gccgatgcca acgtggatga ggttttcagc
     1501 cctgtgcagc aagatttggc catcaaggaa gctccacgcc cacctgctga aacctaaatc
     1561 caaaatcaaa atacatgggc agcacttgta acccaccgtt ctgcccatat atgacaacca
     1621 ttgttaacac tgaatttgtc caaccaacat gagtcaacgc gatttttatg aagtgttagg
     1681 ggtatccaaa ggcgccagcg aagctgaaat caaaaaagcc tatcgcaaat tggcgatgaa
     1741 ataccatcct gaccgcaatc cagacgataa agaagccgaa gaaaaattca aagaaattca
     1801 aaaagcttac gatatcctgt ccacccctga gaaaaaagcc gcttacgacc aatacggtca
     1861 tgccggtgtt gaccctaata tgggcggtgg tggcggtttc ggcggcggct ttggtggcgc
     1921 tgatttcgac atcaatgata ttttcagcca aatgtttggt ggtgctggcg gtggtggtcg
     1981 ttcacgtcag cctaactacc aaggtgcgga tttgcgctat gatttggcaa tttctttgga
     2041 agaagccgct catggcgtga aaaagaaaat cacgtttgcg acgtttgaag agtgtgatgt
     2101 gtgccatggt tcaggcgcga aagcaggctc tgaacctgtg acttgccaca cttgtcatgg
     2161 cagcggcacg gtacacatcc gccaagccat tttccaagtg cagcaaacct gtccaacctg
     2221 ccaaggttcg ggtgtggaaa tcaaagaccc atgtgtcaaa tgtaaaggcc acggcaaagt
     2281 gcgctctagc cgcaccttgg aagtaaacgt gcccgcaggt gtggacactg gcagccgcat
     2341 ccgcttgagc ggcgaaggcg aagcaggcac gcacggcgca ccatcgggtg atttgtacgt
     2401 gttcatcgaa gtgaaagaac acaatatctt ccaacgtgat ggcatggatt tgcattgcga
     2461 agtaccaata ggctttacca ctgctgcctt gggtggcgac gttgaagtgc caaccattga
     2521 tggccgcgtg atattgaaaa ttgcgcctga aacccaattc ggccgtaaaa tgcgcatcaa
     2581 aggcaaaggc atcaagtcct tgcgctctag cagtgtaggc gatttgtatt gccacatcgt
     2641 ggtcgaaacc ccagtgaatt tgaccgagcg tcaaaaagaa ttgttggaag agtttgaaac
     2701 catttccacc ggccaagagc gctctc
//
LOCUS       DAOMVK010000309         2725 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_11052_length_2725_cov_2.668914, whole genome shotgun sequence.
ACCESSION   DAOMVK010000309 DAOMVK010000000
VERSION     DAOMVK010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11052_length_2725_cov_2.668914"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /gene="leuB"
                     /locus_tag="PK856_09430"
     CDS             <1..826
                     /gene="leuB"
                     /locus_tag="PK856_09430"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HRL02629.1"
                     /translation="RPERGLLAIRKDLNLFANLRPAILYPELANASTLKPEVVAGLNI
                     LIVRELTGDIYFAEPRGISQLADGTREGVNTMRYNETEIRRIGHIAFQAAQKRNKKLC
                     SVDKANVLETTELWKQIMTEISADYPEVELSHMYVDNAAMQLVKAPKQFDVMVTGNIF
                     GDILSDEASMLTGSIGMLPSASLNETGKGMYEPSHGSAPDIAGQDKANPLATILSAAM
                     MLRYSFNQDAFADEIEQAVQTVLKQGLRTADIFEEGCTLVGCTAMGDAVVAALQAA"
     gene            826..2142
                     /locus_tag="PK856_09435"
     CDS             826..2142
                     /locus_tag="PK856_09435"
                     /inference="COORDINATES: protein motif:HMM:NF016301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HRL02630.1"
                     /translation="MINRHVWQRWWRLKRYLNIANLPAHDVACPCCGIQVSLPPLGQA
                     QRADCPQCGELLVRIQSNPFVAPLAFVTAGWILMFLVGTQFYLGMDMMGVVSEHLSLV
                     DVFDNLRRHEYGFLATVMLLLVFFTPVLFLSMFMYVHIALYQQLNLPFLNTITRHMIR
                     LKPWLMVDVFFIATLVAIVKIAAISEVSFGPAFVLIFAYAVILIHMGQMVNPHWLFYQ
                     LAKLDPEHKVFEHIPHKHSCISCTRCLYDQPASQEDCDICGASLYHRRPYSIQQSLAI
                     LIAACILFIPSNVYTMMRTESLTADVQSNVIDGIFILWNENDRLVAVIIFTVSLMIPI
                     AKIIMMGILLYSAKYKLLLPAKILSKMYHFVEFIGRWSMIDIFVIIILMAMYRTNIAQ
                     VTPGLASIYFTFVVFATMVSAELLDIRLIWDKQRHPEVAGEEHERV"
     gene            2129..>2725
                     /locus_tag="PK856_09440"
     CDS             2129..>2725
                     /locus_tag="PK856_09440"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRL02631.1"
                     /translation="MNESEQEHSPEPQEPQEEVLETQEAQAPQGMAAATIKKTRAFSI
                     VWVIPIVALLVAVFLLVSYIHNNGTEITLYMPNADGIEVNKTPIKILNVEVGKVVDVR
                     LNGEDGVILTAKVKATIADKLRKDSQFWIVKPRIDQGGVRGLDTLVSGSYIGFKPGQA
                     EDTKHEFVVSDNPPLDDLGDGLRLRLVSDKAKMLPVGAP"
BASE COUNT          675 a          672 c          662 g          716 t
ORIGIN      
        1 gcgccctgag cgcggcctgc tcgccatccg caaagatctg aatctatttg ccaatttgcg
       61 ccctgcgatt ttgtacccag aattggccaa tgcctctact ttgaaacccg aagtagtagc
      121 gggtttgaac attttgattg tgcgcgaatt aactggcgac atttattttg ctgagcctcg
      181 cggcatcagc caattggccg atggtacccg cgaaggcgtg aacaccatgc gctacaatga
      241 aaccgaaatc cgccgcatcg gccacatcgc ttttcaagcc gcgcaaaaac gcaataaaaa
      301 actgtgttca gttgacaaag ccaatgtgtt ggaaaccaca gaattgtgga aacaaatcat
      361 gaccgaaatc agcgccgact acccagaagt ggaattgagc cacatgtatg tggacaatgc
      421 agcaatgcaa ttggtgaaag caccgaaaca atttgatgta atggtcacag gcaatatttt
      481 tggtgacatc ttgtctgatg aagcgtctat gttgacgggt tctatcggta tgttgccttc
      541 ggcttctttg aatgaaacag gcaagggcat gtacgagcct tcacatggct cagctcctga
      601 catcgcaggc caagacaaag ccaatccttt ggcaaccatt ttgtcagcag cgatgatgtt
      661 gcgctattca ttcaaccaag atgcgtttgc tgatgaaatt gagcaagcgg tgcaaaccgt
      721 attgaagcaa ggtttgcgca cggccgacat ttttgaagaa ggctgtacat tagtgggctg
      781 tactgccatg ggcgatgccg tggtggcagc gttacaggcc gcataatgat caatcggcac
      841 gtttggcaac gatggtggcg actcaaacgc tacctgaaca ttgccaacct gcctgcccat
      901 gatgtggctt gtccatgttg tggcatacag gtgagtttac ctcctttggg ccaagcccaa
      961 cgtgccgact gcccccaatg cggggaatta ttggtcagaa ttcaatccaa cccgtttgtc
     1021 gcgcctttgg cgtttgtcac cgcaggttgg attttgatgt ttttggtagg cacccagttc
     1081 tatttgggca tggacatgat gggcgtggtg tctgagcact tgagcttggt cgacgtgttc
     1141 gacaatttgc gccgccatga gtatggtttc ctggccacgg tcatgttgtt attggtgttc
     1201 ttcaccccag tgctgttttt atcgatgttc atgtatgtgc acattgccct ttatcagcaa
     1261 ctcaacctgc ccttcctcaa taccattacc cgccacatga tcaggcttaa gccttggttg
     1321 atggtggatg tgtttttcat tgccactttg gtggccatcg tcaaaattgc tgccatcagt
     1381 gaagtgagtt ttggtcccgc ttttgtgttg atttttgcct atgcggtgat tttgatccac
     1441 atggggcaaa tggtgaatcc gcactggctg ttttaccaac tggccaaact cgatcccgaa
     1501 cacaaggtgt ttgaacacat cccacacaaa cacagctgca tctcctgcac ccgctgtttg
     1561 tatgatcagc ccgcctcaca agaagactgt gatatttgcg gtgccagctt gtatcaccgc
     1621 cgcccataca gcatccagca atcactggcc atcttgatag cggcctgtat tttattcatc
     1681 ccctccaatg tctataccat gatgcgcacc gaaagcctca cagccgatgt gcaaagcaat
     1741 gtgatagatg ggattttcat tttgtggaat gaaaacgaca ggttggttgc tgtcattatt
     1801 ttcactgtca gcctgatgat tccaattgcc aaaatcatca tgatgggcat tttgctgtac
     1861 agcgccaaat acaaattgct gttgcctgcc aagattttga gtaaaatgta ccactttgtt
     1921 gaattcatcg gccgttggtc gatgatagac atttttgtca tcatcatttt gatggcgatg
     1981 taccgcacca atatcgccca agttacccca ggattggcct cgatatactt tacctttgtg
     2041 gtgtttgcca ccatggtgtc ggccgagttg ctcgacatcc gcctaatatg ggacaaacaa
     2101 cgccatcctg aagtggctgg agaagaacat gaacgagtct gaacaagaac acagccctga
     2161 acctcaagag cctcaagaag aagtgctaga aactcaagaa gcgcaagcac cccaaggcat
     2221 ggcggcggcc accatcaaga aaacccgagc attctctatt gtttgggtga ttccgattgt
     2281 cgccttattg gtggctgtgt ttttgttggt cagttacatt cacaacaatg gcactgaaat
     2341 caccctttac atgcccaatg cagacggcat tgaagtcaat aaaaccccga tcaaaatcct
     2401 caatgttgaa gtgggaaaag tcgtggatgt gcgcttaaat ggtgaagatg gcgtcatcct
     2461 cacggccaaa gtcaaggcca ccattgccga caaattgcgc aaagactctc agttttggat
     2521 agtaaaaccg cgcatagacc aaggcggcgt acgaggcttg gataccttgg tgtcaggttc
     2581 ttacattggt ttcaaaccag gccaagccga agacaccaag catgagtttg tggtcagtga
     2641 caatccaccc ctagacgatt tgggcgatgg cttgcgcctg cgcctggtca gcgacaaagc
     2701 caaaatgctg cctgtgggcg cgcca
//
LOCUS       DAOMVK010000310         2698 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_11206_length_2698_cov_2.366629, whole genome shotgun sequence.
ACCESSION   DAOMVK010000310 DAOMVK010000000
VERSION     DAOMVK010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11206_length_2698_cov_2.366629"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PK856_09445"
     CDS             complement(<1..480)
                     /locus_tag="PK856_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02632.1"
                     /translation="MSSLKHGFILTVGLLGYTVWAQAQVVGIDQWLSRKAMVCDVNNT
                     HLLQLSTAVTHSDDTFHYRYDANKQQLLVDKIAYQGVRAAEVLKYGNSSQLALAVSQP
                     RFGLQVIKVAVNLTGQPYEGMGYYWVAQGSIKQNLPALKQLLTERELTGGMVSETAQG
                     "
     gene            773..1321
                     /locus_tag="PK856_09450"
     CDS             773..1321
                     /locus_tag="PK856_09450"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HRL02633.1"
                     /translation="MNIIAFGTSTSQHSINQKLATYVAGELGEHQVRILELSDYEMPI
                     YSIDLQQQGFPSQAHEFLNEIQAADGIVVSLAEHNLNFTTAFKNVLDWVSRIDMQFLL
                     GKKLLVLSTSPGGFGGGNVMEIGLKYFGFAQGEVVAHYSLPSFQDHFKDGVIESEDIQ
                     AIVAEKIDVFLSSLNQTEATTA"
     gene            1665..>2698
                     /locus_tag="PK856_09455"
     CDS             1665..>2698
                     /locus_tag="PK856_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HRL02634.1"
                     /translation="MEQWVNTIAGYVWGNALVVLALAVGLYFTVMTRGVQFRCLRESI
                     RLLTEKRESAEGVSSFQAFCMALSGRVGVGNIAGVATAIAAGGPGALLWMNVMALLGS
                     ASAFIESTLAQVYKHRADGQYRGGSPYYIEKGLKLKGFAVFVAIVITLCYGVLVPGVQ
                     ANTIADSFRNAFDLPPYITGIIVTGLLALIIFGGVKRIARVADKVIPLMAAAYILLMV
                     IILGSHLSAIPEMIVTIVKSAFGMDAVFGGIVGQAIAWGVRRAVFSNVAGAGEATFSS
                     AAAEVSHPAKQGLVQSFSVYIDTVVVCTATGLMILATGMYNVIPAGSTTPLVENIPGI
                     EAGTAYTQAAV"
BASE COUNT          632 a          687 c          660 g          719 t
ORIGIN      
        1 gccttgcgcg gtttccgaca ccatgccacc cgtcagttca cgctctgtta acagctgttt
       61 taaggcaggc aagttttgtt tgatcgagcc ttgcgccacc caataatagc ccatgccttc
      121 atacggttgc cccgttaggt ttaccgccac tttaatcact tgcaaaccaa accgcggctg
      181 ggacacagcc aaagccaatt ggctgctgtt gccatatttc aatacctcag ccgcacgcac
      241 gccttggtag gcaatcttgt ccaccagcaa ttgctgcttg tttgcatcat agcggtagtg
      301 gaaagtgtca tcactgtggg tgactgcggt gctcaattgc agtaaatggg tgttgttcac
      361 atcgcacacc attgctttgc gactgagcca ctggtctatg cccaccactt gggcttgtgc
      421 ccacaccgta tagcccaaca aacccactgt taaaatgaag ccatgtttga ggctgctcat
      481 gcgattccac ttcatattat tgcttggtca cacatactgg cacacaccga atttgctttg
      541 caagccctgc aagcgccaca gcctgtaaat cacagcttgc ttgctggctt acacatgttt
      601 gtctaatggt ctgcccgccc gatttggcat catcaatcgc cactgactgt ttctcatcca
      661 taaaggttta ttgccttgtt aagcatttcc atttgaacgc atttctctta aaatgtgcgc
      721 tgcaagaaag actgcttcaa caacattcat tccaaacatc aaaaggcata atatgaacat
      781 cattgcattt ggcaccagca ccagccaaca ttccatcaac caaaaattgg caacgtatgt
      841 ggcgggcgaa ttgggcgagc accaagtgcg cattttggag ctgagtgatt atgagatgcc
      901 gatttacagc atcgatttgc aacaacaagg ctttccttca caagctcatg aatttttgaa
      961 cgaaatccaa gccgctgatg gcattgtggt gtctttggct gagcacaatc tgaatttcac
     1021 caccgcgttt aaaaatgtgt tggattgggt gtctcgcata gacatgcagt ttttattggg
     1081 caaaaaactg ttggtattgt cgacttcacc aggtggtttt ggcggtggca atgtgatgga
     1141 aatcggtttg aaatactttg gctttgccca aggcgaggtg gtggcacatt attctttgcc
     1201 cagcttccaa gaccatttca aagacggcgt gattgaaagc gaagacatac aagccatcgt
     1261 ggctgaaaaa atcgatgtgt ttttaagcag cttgaaccag actgaagcca caactgctta
     1321 agccctagcc ctatcttaaa tgcggcctgt aaagcttaca ggccgcattt ttatgtccat
     1381 aaacaacctg tttaacgcac actcttctgc aaaacccacc agactcgcca cgcagttcac
     1441 gccagtccca cccatcattt tggttttaat atttcgtcaa aatccgacaa ttatgccgtt
     1501 ttcggcaaaa ctttgtttaa tcccatcaag acagaccttg tttttgcttc gatactaaaa
     1561 aagagaaaac ccgtcatttg tgataaccat aacaacacac aaaggcgcgc atgcaatcaa
     1621 taaagtgaat gtgtcgatat tcacagcatc aaaggagatg tgaagtggaa caatgggtaa
     1681 ataccatcgc aggttacgtt tggggcaatg ccttggttgt gcttgccttg gccgtgggct
     1741 tgtattttac ggtgatgacg cgcggcgtgc agtttcgctg cctgcgcgaa tcgatacgtt
     1801 tgctgactga aaaacgcgaa tcggccgaag gcgtgtcttc gttccaagcc ttttgcatgg
     1861 ccttgtctgg tcgtgtcggt gtgggcaata ttgcgggcgt cgccacggcg attgccgcag
     1921 gcggccctgg tgccttgctg tggatgaatg tgatggcctt gcttggcagc gccagcgcgt
     1981 ttattgaatc gacgttggcg caggtgtaca aacaccgtgc cgatggccaa taccgcggcg
     2041 gctcgccgta ttacatcgaa aaaggcttga agctcaaagg ctttgcggtg tttgtggcca
     2101 tcgtcatcac cttgtgttac ggcgtgttgg tgccaggcgt acaggccaac accatcgctg
     2161 attcattccg caatgccttt gatttgccgc cttacatcac gggcatcatc gtcactggac
     2221 tcttggcgct gattattttc ggcggcgtga aacgcattgc ccgtgtggcc gacaaagtga
     2281 ttccgttgat ggccgctgct tacatcttgt tgatggtcat cattttgggc tcgcacttga
     2341 gcgccatacc tgaaatgatc gtaaccatcg tcaaaagcgc gtttggcatg gatgcagtat
     2401 ttggtggcat tgtcggtcaa gcgattgctt ggggcgtgcg ccgtgcggtg ttctctaacg
     2461 tggcaggcgc aggcgaagcc acgttcagct cggcggctgc cgaagtgtca caccctgcca
     2521 aacaaggctt ggtgcaaagc ttttcggtct acatcgacac cgtggtggtg tgtaccgcca
     2581 caggcttgat gattttggcc acaggtatgt acaacgtgat tcctgctggc tcgaccaccc
     2641 cattggtgga aaacatccct ggcatcgaag cgggcacggc ttacacccaa gcggcagt
//
LOCUS       DAOMVK010000311         2692 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_11225_length_2692_cov_6.429655, whole genome shotgun sequence.
ACCESSION   DAOMVK010000311 DAOMVK010000000
VERSION     DAOMVK010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11225_length_2692_cov_6.429655"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="PK856_09460"
     CDS             <1..1153
                     /locus_tag="PK856_09460"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HRL02635.1"
                     /translation="AYTVNVDERLFVLVQGRLERILARGHYVLNLDDSTVKVLFAKNQ
                     PRLYADTAQIDAWAADDAALIAAHFECVQAAENEVVLCNYQHGLQHIVKPNTRTYFWK
                     NEQEPLLKERISIPADLQLDARTLTQFQAADYQKRPELNALVQSIDVPEQHQAIVSID
                     HQHPGMLDAGKYYFWHLYQDLSWKIVDLRLQTVEVSGQELLSADKVTIRVNVVCHYQV
                     VDSALWVSQYANATDYVYRELQFAVRAVVGSRSIDELLADKQAVDEALVEHMVNKHIA
                     GITIDSMGLKDIILPGEIRQILTKVVEAEKSAQANNIRRREETAATRSLLNTARVMEE
                     NPTALRLKELETLEKVTEKIDKISVFGGLDGVLNGLINIQAPNQNNPSK"
     gene            1234..2160
                     /locus_tag="PK856_09465"
     CDS             1234..2160
                     /locus_tag="PK856_09465"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRL02636.1"
                     /translation="MSVKLPKLSLRQLHIFVAIAEQGATTAASEAIALSQSAVSAALH
                     ELERLMGTQLFDRVGKRLVLNESGRILLQHARPLLDMAKQIEASLDGNAKVLHSARLG
                     ASTTIGNYLLPKLLWQLAQDTTDSAEWNTEINILNTADICQQIAQFELDIGLIEGPCA
                     DPMLQVHPWRSDEMVVVLSPSHPLAGVVSEQGQVDAKHLQAATWLLREQGSGTRAVSD
                     QMLLPVLGQYANRLELSSSEAIKRCALVGLGVACLSRWVVEEELLTGRLVVLEHVFPV
                     TTRMCSWVCHQNKFFNQALLQVLQGLDCTIST"
     gene            complement(2277..>2692)
                     /locus_tag="PK856_09470"
     CDS             complement(2277..>2692)
                     /locus_tag="PK856_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HRL02637.1"
                     /translation="GWKVAKRMDTDLVMDALEQALHARNKPKHLIHHSDRGSQYLSIR
                     YTERLQAAQVKASVGTTGDSYDNALAETVNGLYKTEVIHHQKPWKRMIDVELATLNWV
                     DWFNHRRLLSSIGYVPPAEFEMAYYRQLEEPAKVA"
BASE COUNT          707 a          582 c          693 g          710 t
ORIGIN      
        1 agcgtatacc gtcaatgttg atgaacgctt gtttgtgttg gtgcaagggc gtttagaacg
       61 gattttggca cgggggcact atgtgctgaa tttggacgac agcacagtga aagtcttgtt
      121 tgccaagaac cagccacgtt tgtatgcaga cacggcacag atagatgctt gggcagcgga
      181 tgatgctgct ttgattgcgg cccattttga atgcgtacag gccgctgaaa atgaagtggt
      241 gttgtgtaat tatcaacacg gtttgcaaca catcgtgaag cccaataccc gcacttattt
      301 ttggaaaaat gaacaagaac ctttgttaaa agagcgcatc agcatacctg ctgatttaca
      361 gctggatgcc agaaccttaa ctcaatttca agcagctgat tatcaaaagc ggcctgaatt
      421 gaatgcgctg gtgcaaagca tagacgttcc tgagcaacac caagccattg tcagcataga
      481 ccaccaacac ccgggtatgt tggatgcagg taaatattat ttttggcact tataccaaga
      541 cctcagctgg aaaatcgttg atttgcgttt gcaaacggtg gaagtcagtg gtcaagaatt
      601 gctcagtgca gataaggtga ccatacgtgt caatgtggtg tgtcattacc aagtggttga
      661 cagcgcattg tgggtgagcc aatacgccaa tgccaccgac tatgtttacc gtgaattgca
      721 attcgctgtg cgtgctgttg tgggcagccg cagcattgat gaattgttgg cggataagca
      781 agcggttgat gaggctttgg tggaacacat ggtcaataaa cacatcgctg gcatcacaat
      841 agacagcatg ggtttgaaag acatcatact ccctggtgaa atccgtcaaa ttttgaccaa
      901 agtggtggag gcagaaaaat cggcacaagc aaacaacatc cgccgacgag aagaaacggc
      961 ggccacgcgc tctttgctca acaccgcacg cgtgatggaa gaaaatccaa ctgctttgcg
     1021 tttaaaagag ttggaaacct tggaaaaagt cactgagaaa attgacaaaa tctccgtttt
     1081 cggtggtttg gatggggtat tgaatggctt gatcaacatt caagcgccca atcaaaataa
     1141 cccaagcaaa tgatgtcatg tggatcaaga gatgggttgg gttgaaacaa atatgagcca
     1201 ttttgcccac agttgtccat caatcaggcg gggatgtcag tgaagctgcc caaactgtcc
     1261 ttaagacagc tgcacatttt tgttgccatt gcggagcaag gtgcgaccac tgccgcgagt
     1321 gaggcgattg ctttgtcgca atcggcggtg agcgcggcct tgcatgaact agagcgtttg
     1381 atgggtacgc aattgtttga ccgtgtgggc aagcgtttgg tgttgaatga aagtggtcgg
     1441 attttgttgc aacatgccag accgttgctg gacatggcca agcaaatcga agcttcatta
     1501 gatggcaatg ccaaggtttt acacagcgcg cggttgggag cgagtaccac catcggtaac
     1561 tatctgttgc caaaattact atggcaattg gctcaagaca ccaccgactc tgctgagtgg
     1621 aacacagaaa tcaacatctt gaacaccgcg gacatttgtc aacaaattgc ccaatttgaa
     1681 ttggatatag gcctgataga aggcccttgt gcggatccga tgttgcaagt gcatccgtgg
     1741 cgcagtgatg agatggtggt ggtattgtcc ccatcgcatc ctttggcggg tgtggtgtct
     1801 gaacaagggc aagtggatgc caagcattta caggccgcta cttggctgct gagagaacag
     1861 ggttcgggca caagagcggt ttccgatcag atgctgctgc ctgtattggg acaatatgcc
     1921 aaccgtttag agctcagcag ttctgaggcg attaaacgct gtgctttggt gggtttgggc
     1981 gtggcttgtt tgtcgcgttg ggtggtggaa gaggagttgc ttacaggccg cttggtggtg
     2041 ttggagcatg tttttcctgt taccacccgc atgtgttcat gggtgtgcca tcaaaataaa
     2101 ttctttaacc aagccttatt gcaggtgtta caaggtttgg actgcactat atctacttaa
     2161 atatcaggat ctacctaaat atcaggtaga tataaccata taaattcatt ttataagtaa
     2221 atcatttggg tatacagtga accgacccga ttatttcgga gacagtttat cttgagtcat
     2281 gccactttgg ctggctcttc cagttggcga taatacgcca tttcaaattc tgctggtgga
     2341 acgtaaccta tcgaacttaa caaacgtcga tgattgaacc aatccaccca gtttagtgtt
     2401 gccaattcaa catcaatcat ccgcttccac ggcttttgat gatggatgac ctcagttttg
     2461 tacaaaccat tcaccgtttc agccaaagca ttgtcatacg aatcacccgt tgttccgacc
     2521 gaagccttca cttgagcggc ctgtaaacgt tcggtgtaac gaatcgacag atattggctg
     2581 cctcgatcac tgtggtgaat gaggtgtttg ggcttgtttc tggcatgcaa agcctgctcc
     2641 aatgcatcca tcaccaagtc ggtatccatc cgttttgcca ccttccaacc aa
//
LOCUS       DAOMVK010000312         2664 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_11375_length_2664_cov_3.177463, whole genome shotgun sequence.
ACCESSION   DAOMVK010000312 DAOMVK010000000
VERSION     DAOMVK010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11375_length_2664_cov_3.177463"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..749)
                     /gene="grpE"
                     /locus_tag="PK856_09475"
     CDS             complement(183..749)
                     /gene="grpE"
                     /locus_tag="PK856_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRL02638.1"
                     /translation="MTDSEHKTTAEENEVTGAESAENVADLHQQAEVAEKTAEDWAAE
                     VAELQGQLQDERLRSAANEQNLRRRHEEEIQHTHKFASQKFATELLTVKDYLEMALLD
                     QSQNFDALKMGVSMTLAELEKAFEKAHVSEINPSVGEKLDPHLHQAMQAVESEQEANT
                     VVSVMKKGYSLNERVLRPAMVVVAKAAE"
     assembly_gap    925..934
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1029..>2664
                     /gene="aceK"
                     /locus_tag="PK856_09480"
     CDS             1029..>2664
                     /gene="aceK"
                     /locus_tag="PK856_09480"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HRL02639.1"
                     /translation="MLEGFNKHYTLFHAASARAKPMFEAANWLGIQQLVADRIQMYDE
                     RVHEAVERLRAEVGANALSDDIWQQAKTEYIALLVNHKQPELAETFFNSVSTKILDSM
                     YFSNHFIFVKPAISTEYIDSFPHTFRSFYPNKQGLKGSLHEVLSHFDWQIAFADKKRD
                     IANIMRAAKKHFKQEWPLEEINLHLQVLHSAFYRNKCAYIFGQIISGNQRYPFALAIA
                     HNEDGLLRVDAALFEAEQIAVLFSFARAYFLVEMDVPSAYVEFLHTMLPMRSKGDLYT
                     LLGLQKQGKNIFYREFSRHLDYSNDSFMLAPGIKGLVMLVFTLPSFPYVFKIIKDKFG
                     PNKDFNREYVRQKYLLVKKHDRVGRMADTLEYSNVAIPLARVDAELLAELRTLAASQI
                     EEQGEWLIIKHMYIELRLKPLNMFLQKATIEQKAQAIIDYGNCLKELATANIFPGDML
                     YKNFGMTRFNRVIFYDYDEIEYMTHCNFRDIPEAPNPEYELSDEIWYPVNKGDVFPEE
                     FGPFLLGEPDVRKVFLEHHRDLLTRQFWQSRKDRIES"
BASE COUNT          646 a          676 c          595 g          737 t
ORIGIN      
        1 catctgccgc tcctttgttg tcatcgcttt tttgaaagca aggttgctgt gatatgtctc
       61 acactagcac agctcagtat ggctgcctat aggccatgtt cacaccttag caaacacgaa
      121 aaagcggcct gtaaactgag ttacaggccg cttaatacat ggctatgcca tcttagcaac
      181 aattattctg cggctttggc caccaccacc atggctgggc gcaacacgcg ctcgttcaag
      241 ctgtagcctt ttttcatcac cgaaaccacg gtattggctt cttgttcgct ttccacggct
      301 tgcatcgctt ggtgcaagtg tggatcgagt ttttcaccca cgcttgggtt gatttcgctg
      361 acatgggctt tttcaaatgc tttttccaat tcggccaacg tcatgctcac gcccattttt
      421 aaagcgtcaa agttttggct ttggtcaagc aaggccattt ccaaatagtc ttttaccgtt
      481 aacaattcgg tcgcaaattt ttggctggca aatttgtgcg tgtgttggat ttcttcttca
      541 tggcggcggc gcaaattttg ctcgtttgca gccgagcgca aacgctcatc ttgcaattgg
      601 ccttgtaatt ccgccacttc agccgcccaa tcttcagctg ttttttcagc tacttccgct
      661 tgttggtgca aatctgcgac attttctgct gattctgctc cagttacctc attttcttct
      721 gctgttgtct tgtgttcgct atcagtcatt ttgcatcctt tctcaagtca ttcatgttgg
      781 attgccaatc catgttcaca tttgaaatca attgtcgtgc acatttgggc gattgcgcat
      841 aattcaagcc acatgctgac ttccattctg gatttacagg ccgcttgttg cattgcatca
      901 atagccgctt gtggattaat cttannnnnn nnnnctacaa aaaaaacaat gataagaatg
      961 agtcgccaaa ggagtctgtt gtgaagtcca aactggccca tatacaagcc aaagccgttg
     1021 ccgccatcat gttagaaggc ttcaacaaac attacacctt gttccacgcc gccagcgccc
     1081 gcgccaaacc gatgtttgaa gccgccaatt ggctgggcat acaacaattg gtggccgacc
     1141 gcatacaaat gtacgacgag cgcgtgcatg aagctgtgga gcgtttgcgc gcggaagtgg
     1201 gcgcgaatgc gctatcagat gacatttggc agcaagccaa aaccgaatac attgccttgt
     1261 tggtcaacca caagcagcct gagctggccg agaccttctt caactcggtt tccaccaaaa
     1321 tcttggacag catgtatttc agcaaccact tcatttttgt gaagcccgcg atttccaccg
     1381 aatacataga ctcttttccg cacacattcc gctcgtttta tcccaataag cagggattga
     1441 aaggcagttt gcacgaagtc ttaagccatt ttgattggca aatcgccttt gccgataaga
     1501 aacgcgacat cgccaacatc atgcgcgccg ccaagaaaca cttcaaacaa gaatggccac
     1561 tggaagaaat caatttgcat ttgcaggtat tgcattcggc gttttaccgc aacaaatgtg
     1621 cctacatttt tgggcaaatc atcagtggca accaacgcta tccctttgcc ttggccattg
     1681 cccacaatga agacggtttg ctgcgcgtgg acgctgcctt gtttgaggcc gagcaaattg
     1741 cggtgttgtt ctcgtttgca cgggcgtatt ttttggtgga aatggacgtg ccttcggcgt
     1801 atgtggaatt tttgcacacc atgttaccaa tgcgttccaa aggcgatttg tacaccttgc
     1861 taggcttgca aaagcaaggt aagaacattt tctatcgcga gttttcgcgc catttggact
     1921 attccaatga ctctttcatg ctcgcgcctg gcatcaaagg cttggtgatg ttggtgttca
     1981 cgctgccctc gtttccctat gtgttcaaaa tcatcaaaga caaatttggc cccaataaag
     2041 actttaaccg tgagtatgtg cgccaaaaat acttactggt gaaaaaacac gaccgtgtgg
     2101 ggcgcatggc cgataccttg gaatactcca atgtggccat tccattggca cgggtcgatg
     2161 ccgaattgct ggcagaattg cgcactttgg ccgccagcca aatcgaagaa caaggcgaat
     2221 ggctgatcat caaacacatg tacatcgaac tgcgcttgaa gccgctgaac atgtttttgc
     2281 aaaaagccac catcgagcaa aaagcccaag ccatcattga ttatggcaat tgcctgaaag
     2341 agctggccac cgccaatatt ttccctggcg acatgttgta taaaaacttt ggcatgaccc
     2401 gttttaaccg cgtcattttc tacgactatg atgaaatcga gtacatgacg cattgcaatt
     2461 tccgcgacat tcccgaagcg cccaatccag aatatgagtt atccgatgaa atctggtatc
     2521 ccgtgaacaa gggcgacgtg tttcctgaag agtttggccc gtttttgctg ggcgaacccg
     2581 atgtgcgcaa agtgttcttg gaacaccatc gcgatttgct cacgcgccag ttttggcaat
     2641 cgcgcaaaga ccgcatcgaa agcg
//
LOCUS       DAOMVK010000313         2650 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_11466_length_2650_cov_2.168786, whole genome shotgun sequence.
ACCESSION   DAOMVK010000313 DAOMVK010000000
VERSION     DAOMVK010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11466_length_2650_cov_2.168786"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PK856_09485"
     CDS             <1..638
                     /locus_tag="PK856_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRL02640.1"
                     /translation="LQQTGFLQLDLQGKVICGQRTHQLALNLWQTSHYRVERQAILQS
                     LASEIGETVGIAVMHDLDIVYIDRVLSNWPLQIYLPEGMQIPIWASASGKLLLAQLPP
                     EQYTRVVDKLSIYALTTNTQTDRQQLLATIARAKAEQLSVDNEEFIPGMVAFAVPIPN
                     GDGAPFATLFTHGPTVRKSLDELLTYTPAMRQAAEELAAVFQKQGAEAFNN"
     gene            complement(726..1985)
                     /locus_tag="PK856_09490"
     CDS             complement(726..1985)
                     /locus_tag="PK856_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter"
                     /protein_id="HRL02641.1"
                     /translation="MSQNSAVKTPSLLGGMMIVAGTAIGAGMFANPTATAGVWYVGSI
                     VVMAYTWFCMCASGLMIMEANQHYPAGASFNTMVKDLLGPVWNAINGLAFVFVIYILL
                     YAYIFVGGSMLEPFVAQLGFGSASAKIAATVFVLAFALCVWWSTQAVDRVSTVLIVAM
                     VITFITSVTGMLNQLSVATLMNALPEGAGSVYLPYVWVALPVCLASFGFHMNVPSLMA
                     YYQNNTHKVRTSVVGGTLIALAIYCLWQTAVQGVLPRHEFGPVIAAGGDVPVLLKALS
                     THIDIAGLNQVLNAFAFMAIVSSFLGVSLGLFDYAADMFHFKANRTGRLKSAAITFLP
                     PYVACILFHTGFVTVIGYVGLGAAVWTAIIPVLMVVASRRRFAVQTGVYRIGGGKALL
                     WFVFIFGMANIVAQVLTQLGWLPTFKG"
     gene            complement(2379..>2650)
                     /locus_tag="PK856_09495"
     CDS             complement(2379..>2650)
                     /locus_tag="PK856_09495"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957660.1"
                     /note="catalyzes the formation of arginosuccinate from
                     citrulline and aspartate in arginine biosynthesis; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRL02642.1"
                     /translation="GEVTLELRRGNDYSIMNTESANLTYQAERLTMEKGDSMFSPADR
                     IGQLTMRNLDIVDTRAKLGIYADAGLLSLGQGSALPQLSNKKEDK"
BASE COUNT          773 a          728 c          574 g          575 t
ORIGIN      
        1 aattgcagca aacaggtttt ttgcaattgg atttgcaagg caaggtgatt tgcgggcaac
       61 gcacccacca attggcattg aatttgtggc agaccagtca ttaccgtgta gagcgccaag
      121 cgatactgca atcactagcg agtgaaattg gcgaaaccgt cggcattgcg gtgatgcacg
      181 acttggacat tgtttacatc gaccgcgtgt tgtcgaattg gcccttgcaa atttacctgc
      241 ccgaaggcat gcaaatccca atttgggcca gtgccagtgg caaattattg ttggcacaat
      301 tgccgcctga gcaatacacc cgtgtggtgg acaagctgtc gatttacgcc ctcaccacca
      361 atacccaaac cgacagacaa caattgctcg ccaccatcgc ccgcgccaaa gccgagcaac
      421 tgagcgtgga caatgaagag ttcatcccag gcatggtggc cttcgccgtg ccgataccca
      481 atggcgacgg cgcaccattt gccactttgt ttacccatgg cccgaccgtg cgcaagtcct
      541 tagacgaatt gctgacttac accccagcca tgcgacaagc tgccgaagaa ttagcagccg
      601 tgtttcaaaa acaaggcgcg gaagcattca acaattaagc acatcttgca tacaaaccat
      661 acaagcggcc tgtaaacact gaatgtgctt acaggccgct tgtattgcat tttaagtatg
      721 ggttttcaac ccttaaacgt gggcaaccag cccaattggg tcaagacctg cgccacaata
      781 ttggccatgc cgaaaatgaa cacaaaccac aacaaagcct tgccaccacc gatgcggtaa
      841 accccagttt gcaccgcaaa acggcggcga ctggccacca ccatcaacac aggaataatg
      901 gccgtccaca ccgccgcgcc caagcccaca taaccaatca cggtgacaaa acccgtgtga
      961 aacagaatgc acgccacata aggcggcaaa aaggtgatgg cagccgattt taagcggcct
     1021 gtacggttgg ctttaaaatg aaacatgtcg gcagcgtaat cgaacaaacc caaagacacg
     1081 cccaagaatg agctgacgat ggccatgaag gcaaacgcat tcaacacttg attcaaaccc
     1141 gcaatgtcta tgtgagtcga taaggctttt aacagcacag gcacatcgcc gcccgccgca
     1201 atcacagggc caaactcatg ccgcggcaac accccttgca ccgccgtttg ccacaagcag
     1261 taaattgcca aggcaatcaa ggtaccaccc accacactgg ttcgcacttt gtgggtattg
     1321 ttttggtaat acgccatcaa actgggcaca ttcatgtgaa agccaaatga ggccagacac
     1381 acaggcaaag ccacccacac ataaggcaag taaacgctgc ccgccccttc aggcaaggcg
     1441 ttcatcaatg tggccacact caattggttc aacatgccag tcaccgaggt gataaaggta
     1501 atcaccatcg ccactatcaa cacggtcgat acgcggtcta ccgcttgggt tgaccaccac
     1561 acacacagtg caaatgccaa cacgaacaca gtggctgcaa tttttgcact ggcagaaccg
     1621 aagcccaact gcgccacaaa aggctctaac atgctgccgc ccacaaaaat ataggcatac
     1681 agcaagatgt aaatcacaaa cacaaacgca aggccattga tggcattcca cacaggccca
     1741 agcaagtctt tgaccatggt attgaagctc gcgcccgcag gataatgctg attggcctcc
     1801 atgatcatca aacccgaggc acacatgcaa aaccatgtgt aagccatcac cacgatggag
     1861 ccgacatacc acacgcctgc cgtggccgtg gggttggcaa acatgccagc accgatggcg
     1921 gtgcccgcaa caatcatcat accacccaat aatgagggcg tttttactgc tgaattttgt
     1981 gacattttgg aacaattact aagataagcc gaatatttta gcatgactgc caaaatcgct
     2041 ttattgatac agtccgtcca actcgcttct acagacttct cgccatcagc agcattgccg
     2101 aaaataaatg attattgtta tgataaattt aaaaaccatt aaaatttgga catattgacc
     2161 aatttaatac ttaatgacac agctatattg ctattcatat catcaatatc cagccataaa
     2221 gtttacaggc cgctgcaagc tactcatagc actcaaagaa acactctcat tgttctaata
     2281 cgccacgctt gctgattcag catcctgaag gctttcatga taaaaccccg tttacaagcg
     2341 gcctgtaaac ggggttttca tatcatgcaa tcagaagctt atttgtcttc ttttttgtta
     2401 ctcaattgtg gtaaggcaga gccttggcct aaagacaaca agcctgcgtc agcataaata
     2461 cccaatttgg cgcgggtatc aacgatgtcc aagttgcgca tggtcaattg gccgatacgg
     2521 tcggctggac tgaacataga atcgcctttt tccatggtca agcgttctgc ttggtaagtc
     2581 agattagcag attcggtatt catgattgaa tagtcgttac cgcggcgcaa ttccaaagtc
     2641 acctcgcctg
//
LOCUS       DAOMVK010000314         2642 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_11512_length_2642_cov_4.187862, whole genome shotgun sequence.
ACCESSION   DAOMVK010000314 DAOMVK010000000
VERSION     DAOMVK010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11512_length_2642_cov_4.187862"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..1945
                     /locus_tag="PK856_09500"
     CDS             158..1945
                     /locus_tag="PK856_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRL02643.1"
                     /translation="MAYQAPLNDLRFALHTHGRLPEILALPKSAGLDDETVNAVLEEA
                     AKFAETALADTNRVGDLNGAKLVDGKVVCDPAIAGAFNEFRDAGWVGLRAAAEFDGQG
                     LPMTVAAACEEMWCSANLAFSLLPMLTLGAFEALNHHGSPELKQLFMPNMATGVWSGT
                     MNLTEPQAGSDLAQVACKAKPLGDGTYAIDGQKIFITWGDHELVDNIVHMVLARLPDA
                     APGVKGISLFLVPKYWVEADGSVGAYNNAHAVSLEHKMGIHASPTCVMAFEGARGHLI
                     GEAGKGLNYMFTMMNHARLGVGIEGHAVAERAYQHALAYAHERVQSRSISSDSKEPVS
                     IIHHPDVRRMLLQQKATLEAQRAVYLQAAAALDTAESHPSPDVQKQAQAALDFLIPIV
                     KGWSTENGTTLTNLAMQIFGGMGFIEETGAAQFVRDVRITAIYEGTNGIQALDFIGRK
                     TASNGGVVAKAFIAEGRAVAARLTNEQGLADKLNRALDLAEESVNYIVAQFAGNNADF
                     AAAGSYAYLMQFGFTLGGIEMARAHIAAGKALANESEQDLGEDFYRNKQATARAYIDL
                     VLPQVLAYHEQVLGSAAAIMDYPLSALSV"
     gene            2183..>2642
                     /locus_tag="PK856_09505"
     CDS             2183..>2642
                     /locus_tag="PK856_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HRL02644.1"
                     /translation="MQTTSPIDPITLSVVRGALETAQREMTITLEKTGRSSVFNLAHD
                     YSNALFDHHPEMILQGQDIPIHLGSLIPAMKAVADFFGDDIHEGDVIYHNDPVYAGSH
                     ILDCCMYKPIFHNGKLVFWAVCKGHLTDIGGPVPAGYNPDAKELYAEGLRI"
BASE COUNT          649 a          698 c          679 g          616 t
ORIGIN      
        1 tcattctaac aaaatcggtg acaaactggg gtttagagca ttattcttgt ctgtatacaa
       61 aaataaattc accgtgtcat aatcatgacc atgtaaatga ttgcactaaa tcagcgcttg
      121 tcctaacact aacaatatct ttctcaaagg agagaacatg gcttatcaag caccattaaa
      181 tgatttacgt tttgcattac acacccatgg ccgcctgcca gaaatcttgg ccttgcccaa
      241 atcagcaggt ttggatgacg aaaccgtcaa cgccgtctta gaagaagccg ccaaattcgc
      301 cgaaaccgcc ctcgccgaca ccaatcgcgt gggcgatttg aatggggcaa aattggtgga
      361 tggcaaagtg gtgtgcgacc ctgccattgc aggcgcattt aacgaatttc gcgatgctgg
      421 ctgggtaggc ttgcgcgccg ctgccgaatt cgatggccaa ggcttgccaa tgacggtggc
      481 tgccgcgtgt gaagagatgt ggtgttctgc caacttggca ttttcattgt tgcccatgct
      541 gactttaggc gcatttgaag ccttgaatca ccacggctcg cccgaattga aacaattgtt
      601 catgcccaat atggccacag gcgtgtggtc tggcacgatg aacttaaccg agccacaagc
      661 gggttctgat ttggcccaag tggcctgtaa agccaagcct ttgggcgacg gcacttacgc
      721 cattgatggc caaaaaatct tcatcacgtg gggcgaccat gagttggtcg acaacatcgt
      781 gcacatggta ttggcgcgtt tgcccgatgc agcgcctggt gtgaaaggca tttcgctgtt
      841 tttggtgccc aaatactggg tagaggccga tggcagcgtg ggtgcttaca acaacgccca
      901 tgccgtttcc ttggaacaca aaatgggcat acacgcgagc ccgacttgcg tgatggcttt
      961 tgaaggcgcg cgcggccatt taatcggcga agcgggcaaa ggcttaaatt atatgttcac
     1021 gatgatgaac catgcgcgct tgggcgtggg cattgaaggc catgccgtgg ccgaacgcgc
     1081 ttaccaacac gccttggctt acgcgcacga acgcgtgcaa agccgctcca tcagcagcga
     1141 cagcaaagag cctgtgagca tcatccacca tcccgatgtg cgccgcatgt tgttgcaaca
     1201 aaaagcaacc ctagaagcac agcgcgccgt gtatttacag gccgctgccg cgctggacac
     1261 cgccgaaagc caccctagcc ccgatgtaca aaaacaagcg caagcggctt tggactttct
     1321 gataccgatt gtgaaaggct ggagcaccga aaacggcacc actttgacca atttggcgat
     1381 gcaaattttc ggcggcatgg gctttatcga agaaacaggc gcggcacaat ttgtgcgcga
     1441 tgtgcgcatc accgcgattt acgaaggcac caacggcatc caagccttgg atttcatcgg
     1501 ccgcaaaacc gccagcaatg gtggtgtggt ggccaaagcc ttcatcgcgg aaggccgcgc
     1561 cgtggccgcc agactcacca atgagcaagg tttggccgac aaattgaacc gcgctttgga
     1621 tttggccgaa gagtctgtga attacatcgt ggcgcaattt gcaggcaaca atgccgattt
     1681 tgctgctgca ggcagctatg cttatttgat gcagtttggc tttactttgg gcggcattga
     1741 aatggcgcgc gcccacatcg ccgcaggcaa agccttggcc aacgagagcg aacaagactt
     1801 gggcgaagat ttttaccgca acaaacaagc cactgcccgc gcctacatcg atttggtgtt
     1861 gccacaggtt ttggcctacc acgagcaggt tttgggcagc gctgccgcca tcatggatta
     1921 cccattgtcg gctttgtctg tgtaaaaccc tgatttgtgc acagatttcg catttcaatc
     1981 tgtttgcatc ctaataagcg gcctgtaaac cttttacagg ccgctttctt gtctttttga
     2041 ctctttattc attcatttca acacatttgt tttttagtct gagtgtgcac attgcagcgc
     2101 agcaatgcac ccacagtcaa gacggcggtt tctccacgta atactgttga cccatggcat
     2161 gacaacaacg aggagaaaag acatgcaaac caccagcccc atagacccga ttaccctgtc
     2221 ggtggtgcgc ggcgcactgg aaacggcaca acgtgaaatg accatcactt tggaaaaaac
     2281 cggccgttcc agcgtgttca atttggcgca cgactacagc aatgccttgt ttgaccacca
     2341 tcctgaaatg attttgcaag gacaagacat cccgattcac cttggatcct tgattcccgc
     2401 catgaaggct gtggcggatt tttttggaga cgacatccac gaaggcgatg tgatttacca
     2461 caacgaccca gtgtacgcag gcagccacat tttggattgc tgcatgtaca aaccgatttt
     2521 tcacaatggg aaattggtgt tttgggccgt gtgtaaaggc cacctcaccg acatcggcgg
     2581 ccccgtaccc gcaggctata acccagacgc gaaagaattg tatgccgaag gcttgcgcat
     2641 tc
//
LOCUS       DAOMVK010000315         2642 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_11515_length_2642_cov_2.576730, whole genome shotgun sequence.
ACCESSION   DAOMVK010000315 DAOMVK010000000
VERSION     DAOMVK010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11515_length_2642_cov_2.576730"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1617)
                     /locus_tag="PK856_09510"
     CDS             complement(<1..1617)
                     /locus_tag="PK856_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRL02645.1"
                     /translation="MALFFIKRPVFAWVLAIFIVFLGVLSIKSLPLEQYPDIAPPRVS
                     IAATYNGASAQTVNDSVIQVIEQQLKGLDRLMYMNSTSDSAGRARTTLTFSPGTDIDV
                     AQVQVQNVLQQALSRLPDSVQNQGVRVTKGGQDTLMTWMFTSDDPKVNKVDIGDYLSS
                     NVMDILGRIDGVAEVELYGSPYAMRVWLDPYKLEQYQLMPADISNAISNQNTQVSAGQ
                     LGKLPAVDAQMLSVPIQARSKLRTVAQFEDIIVKSAPDGGIVQLKDVARIELGAESAD
                     IQTKLNGKNGAALGIILADGANALDVADAVTAKIKALEPNFPSGYRATTSQDSTPFVK
                     ASVEEVLKTLLEAIVLVVVVMYVFLQNWRATLIPAVAVPVVLMGTFGVMALFGYSINT
                     LTMFALVLAIGLLVDDAIVVVENVERVMHEQQLDAKTATEVSMKEISSALIGVGMTLS
                     AVFVPMAFFPGSTGVIYRQFAITIIAAMGFSVLVALTLTPAMCAQMLKAKPHTGRSNG
                     LFGRLFASFNRGFERLSLAYQRTVLKLLQCS"
     gene            complement(1844..>2642)
                     /locus_tag="PK856_09515"
     CDS             complement(1844..>2642)
                     /locus_tag="PK856_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HRL02646.1"
                     /translation="LGHTLSQMLRMVQSYGYHQIFFIGLLEQIRAWLRHEETRQLLED
                     NINSWAAKVKKDNPSSWDKLVAAFKGSAVDMVDGWVAGKVLDWADNYCAEVINDDSHA
                     MRAGVERKMSQLVWALEYSPIMHKRLNQAKNGLINSDAVQQFIQHSWLSLQNWSASDA
                     AKSDSMLMQQVLKMMNQMLIQGAAQPEVLRRFDAQISLWVRDAVEQYKSKVADYVAEK
                     VKSWDSQELVQKLELSVGKDLQYIRINGTLVGGLVGLVIYIVSGWLI"
BASE COUNT          670 a          724 c          602 g          646 t
ORIGIN      
        1 ggaacactgc aaaagtttca ataccgtgcg ttggtaggct agtgacaaac gttcaaagcc
       61 acgattaaag ctggcaaaca ggcgaccaaa caagccattg ctgcggcctg tgtgtggctt
      121 ggctttgagc atttgtgcac acatggcggg tgttaaggtg agcgccacca acaccgaaaa
      181 gcccatggcc gcgatgatgg tgatggcaaa ttggcggtat atcacccctg ttgagcctgg
      241 gaagaatgcc atgggcacaa acaccgccga caaagtcatg cccacaccga ttaaggccga
      301 agagatttct ttcatagaca cttcagtagc ggttttggca tcgagttgct gctcatgcat
      361 cacccgctcg acgttttcca ccaccacaat ggcatcatcc acaagcaagc ctatggccaa
      421 taccaatgcg aacatggtca aggtattgat ggaataacca aacaaggcca tgacaccaaa
      481 agtacccatc aacaccactg gaaccgccac ggcaggaatc aaggtagcgc gccagttttg
      541 taaaaacaca tacatcacca ccaccaccaa cacgatggct tccagcagcg ttttcaaaac
      601 ctcttctacc gaggctttga caaagggcgt gctgtcttgg ctggtggtcg ctcgatagcc
      661 agacggaaaa ttcggctcta aggctttgat tttggccgta accgcatcgg ccacatccaa
      721 agcattggca ccatcggcca aaataatgcc caaagccgcg ccatttttgc cattgagttt
      781 ggtttggatg tcggcacttt ccgcacccag ctcaatccgt gccacatctt tcaattgcac
      841 aatgccacca tcgggcgcac ttttgacgat gatgtcttca aactgcgcca ctgtgcgcaa
      901 tttgctgcgt gcttgtatgg gcacactgag catttgtgca tccacagcag gcagcttacc
      961 caattgccct gccgacactt gggtgttttg gttgctgatg gcattgctga tgtcggctgg
     1021 catcaactgg tattgctcca gtttgtatgg atccagccac acccgcatcg cataaggact
     1081 gccatacaat tccacctcag ccacgccatc aatgcgcccc aagatgtcca tcacattgct
     1141 ggacaaatag tcgccaatgt ccactttgtt gactttaggg tcatccgagg taaacatcca
     1201 cgtcatcaat gtgtcttgtc cccctttggt cacgcgcacc ccttggtttt gcaccgaatc
     1261 gggcaagcgc gacaaggcct gttgcaacac gttttgcact tgcacttgcg ccacatcgat
     1321 gtcggtgcca ggtgagaagg ttaaggtggt gcgggctcga cctgctgaat cactggtgga
     1381 gttcatgtac atcaagcggt ccaagccttt gagctgttgc tcgatgactt gtatcaccga
     1441 atcattgacc gtttgtgccg atgcgccatt gtacgtggcc gcaattgaaa cccgcggtgg
     1501 cgcgatgtcg ggatactgct ccaaaggaag gctcttaatg gacaagacgc ccaaaaagac
     1561 tatgaatatt gctaacaccc aagcaaacac ggggcgtttg atgaaaaaca aagccatggt
     1621 ttatacagtc gactatctga cagggatgaa aaatgaagaa gccgccaaca cacggctctg
     1681 gtttaccaca ttgtaagttc aaaaatatct tattgataat gaatcttgtt aaattgtatt
     1741 aaaacaatgc caaacttaag gataagcagc tgcgagcaca gacttgtgtg tcatcaatcg
     1801 tcattaatgg gcttacaggc cgcattcata agcggcctgt aaactatatc aaccagccag
     1861 acacaatgta tatcaccaag cccaccaggc cgcccaccaa agtgccattg atgcggatgt
     1921 attgcaaatc cttgcccacg ctcaattcca atttttgcac caactcttgg ctgtcccaag
     1981 atttcacttt ttcagccaca taatccgcca ctttggattt gtattgctct accgcatcgc
     2041 gcacccacag cgaaatctgg gcatcaaagc ggcgcaatac ctcaggctga gctgcgcctt
     2101 gtatcagcat ttggttcatc attttcaaca cctgctgcat caacatcgaa tcgcttttcg
     2161 ccgcatcgct cgcgctccaa ttttgcaaac tcagccatga atgttggatg aattgctgca
     2221 ctgcatcgga attaatcaaa ccgtttttgg cttggttcaa acgcttgtgc atgatgggag
     2281 aatactccag cgcccaaacc aattggctca ttttgcgctc tacccctgcg cgcatggcgt
     2341 ggctgtcgtc gttgatgact tcagcacagt aattatccgc ccagtccaac actttgcccg
     2401 ccacccagcc atcgaccatg tctacggcgc tgcctttaaa agccgccacc agcttatccc
     2461 aactggaggg attgtctttc ttgacttttg ccgcccatga attgatgttg tcttccagta
     2521 attggcgcgt ttcttcgtgc ctgagccaag cgcgaatttg ttcgagcaaa cctatgaaaa
     2581 agatttggtg gtagccatag ctttgcacca tgcgcagcat ttgcgacaag gtatgcccca
     2641 aa
//
LOCUS       DAOMVK010000316         2634 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_11563_length_2634_cov_2.097325, whole genome shotgun sequence.
ACCESSION   DAOMVK010000316 DAOMVK010000000
VERSION     DAOMVK010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11563_length_2634_cov_2.097325"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..912
                     /locus_tag="PK856_09520"
                     /pseudo
     CDS             3..912
                     /locus_tag="PK856_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749313.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
     gene            968..1044
                     /locus_tag="PK856_09525"
     tRNA            968..1044
                     /locus_tag="PK856_09525"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1002..1004,aa:Met,seq:cat)
     gene            1178..1435
                     /locus_tag="PK856_09530"
     CDS             1178..1435
                     /locus_tag="PK856_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02647.1"
                     /translation="MNSSETNPFALWQQFWQNANPQMAPFLPPMSAEEVQKKISELQS
                     VELWLSFNLQMVQSQLAVLEQQKLFFNSLNQMSENLSTKPD"
     gene            1483..>2199
                     /locus_tag="PK856_09535"
     CDS             1483..>2199
                     /locus_tag="PK856_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRL02648.1"
                     /translation="MTSPRYIPLRMHSEFSIEDGMIRIKEAVKHASNSGFAALGISDL
                     MNLFGMVKFYKACRGAGIKPIIGMDVWVQDDETGEGDNKPYRLMLIARNQPGFKRLCE
                     LITQAYVDDDRDKMDFRLRYSWLKNGDNSGLICLSGAHLGSIGQHFLSGRPEQAKQAA
                     LDLSEMFPNRFYLELQRLPERPEWENAVVGSLNLAQELDLPIVATHPTQFLNKDDFQA
                     HEARVCIASGYVLGDHRRPKN"
     assembly_gap    2200..2209
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2210..>2634
                     /locus_tag="PK856_09540"
     CDS             <2210..>2634
                     /locus_tag="PK856_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRL02649.1"
                     /translation="SQYFVSADEFEARFADLPEALQNSVEIAKRCNVEIVLGENFLPQ
                     FPTPEGMTLDDYLGHLSNEGLADRMAILYPDEAERAARYPEYQQRLDYELHTIIQMGF
                     PGYFLIVQDFINWAKQNGCPVGPGRGSGAGSLVAYALKI"
BASE COUNT          666 a          600 c          663 g          695 t
ORIGIN      
        1 acatgacctt aaccgagtta cgctatattg tggctgtggc acaagagcat cactttggtc
       61 gagcagccca gcgctgcttt gtgagccaac caaccctttc cattgcgatt aaaaaattgg
      121 aagaagagtt gggtgttgcc ttgttcgacc gcagcagcaa cgaaatacgt cctactgatg
      181 cgggtttgcg cattttggat caagcgcaac gcgtgttgga agaagccgca attggccaaa
      241 gaagaacaaa acgagttgga aggttctttc aaactcggtt tgattttcac ggtcgcgccg
      301 tatttattgc ctaagttgat tgtgtctttg cgtgaattgg cacccaaaat gcctttgatg
      361 ctggaagaaa accacaccgc agtcttaacc gacatgctga aaaaaggcga attagacgcc
      421 attgtggtgg ccgatccgta tcaagaggct ggtgtggtga ccataccttt gtacgatgag
      481 cctttctttg tgattacacc caaaggccat gtgtttgagg aaatggacga gatcaccacc
      541 caagagttgg ccgacgaaca ggtattgctg ttgaccgaag gtaattgcat gcgcgatcaa
      601 gtgttggaca gctgtgtgga attggccagt cgtcagcgca tcaagggttt gaccaatacc
      661 ttgcaaggca gctctatcaa taccattcgt catatggtgg catcgggttt gggcatcagt
      721 gtgttgccag caacatcgtt gaccgaaaac gatcacatgt tattcagtat tattccgttt
      781 aaagcacctg cgccagaacg tcgtgtgtta ttggcggctc gccgtcattt cgtgcgtcaa
      841 aaagcattgg acaccataca aaccgctatt ttccattcgc aattggcagg cgtgaagttc
      901 gttaatcaat aaatttaacg aaaacgcttg acgcagtgta gcagatgaat tatagttaca
      961 tttctgacgc ggggtggagc agcatggtag ctcgtcgggc tcataacccg aaggtcgtag
     1021 gttcaaatcc tgcccccgca accagtttct aaaaaccagc ctattggctg gttttttaca
     1081 tttcacgaca ctattttgtc tgagctaagt tacaatgtga tggcagagcg ctttataaat
     1141 gctttcgttt tcgatacaac ttatgcgaag gggtgttatg aattcaagtg agaccaatcc
     1201 atttgcctta tggcaacaat tctggcaaaa cgccaatcca caaatggccc catttttgcc
     1261 acccatgtcc gctgaagaag tgcagaagaa aatttctgaa ttgcaaagcg tcgagctgtg
     1321 gttaagtttc aatctgcaaa tggtacaatc ccaattggcc gtcttagaac agcaaaaact
     1381 gttttttaat tcactcaatc aaatgagtga aaatctcagc accaaaccag attaattccc
     1441 agcggcttta caggccgctt ttatttttat taggttgtac acatgacttc cccacgctat
     1501 atccccttgc gcatgcactc tgagttttcg atagaagacg gcatgatccg cattaaagaa
     1561 gcggttaaac acgccagcaa ttcaggcttt gccgctttag gcatatcgga tttgatgaat
     1621 ctgtttggca tggtcaagtt ttacaaagcg tgtcgcggag cgggtatcaa gcccatcatt
     1681 gggatggacg tgtgggtgca agacgatgaa acaggcgaag gtgataacaa gccttatcgc
     1741 ttgatgctga ttgcgcgcaa ccagccgggt tttaaacgct tgtgcgaact gattacccaa
     1801 gcctatgtgg atgacgaccg cgacaagatg gattttcgct tgcgctattc atggttgaaa
     1861 aacggtgaca acagcggttt gatttgtttg tcgggcgcac atttgggcag catagggcag
     1921 catttcttgt caggccggcc tgaacaggcc aaacaagccg cgctggattt gtctgagatg
     1981 tttcccaatc ggttttattt ggaattgcaa cgcctgcccg aaagacctga gtgggaaaat
     2041 gcggtggtgg gcagcttgaa tttggcgcaa gagctggatt tgcccatcgt ggccacccat
     2101 cccacccaat ttttgaacaa agacgatttt caagcccacg aagcgcgcgt gtgcattgct
     2161 tcgggctatg tgttgggcga ccaccgccgt cctaaaaatn nnnnnnnnnc gtcacagtat
     2221 tttgtttcag ccgatgaatt tgaagcgcgc tttgccgatt tgcccgaagc tttgcaaaac
     2281 tcagtggaaa ttgccaagcg ctgtaatgtg gaaattgtct tgggtgagaa tttcttgcca
     2341 caatttccaa cgccagaagg catgacgctg gacgattatt tggggcattt gtccaacgag
     2401 ggcttggccg atcgcatggc gattctgtac ccagacgaag ccgagcgtgc ggcgcgctat
     2461 cctgagtatc agcagcgctt ggattacgag ctgcacacca tcattcaaat gggttttcca
     2521 ggctatttcc tcatcgtaca agacttcatt aactgggcca aacaaaatgg ctgtccagtg
     2581 ggcccaggcc gtggttcggg tgcgggctcg ttggtggcgt atgccttgaa aatt
//
LOCUS       DAOMVK010000317         2632 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_11575_length_2632_cov_2.190532, whole genome shotgun sequence.
ACCESSION   DAOMVK010000317 DAOMVK010000000
VERSION     DAOMVK010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11575_length_2632_cov_2.190532"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..844
                     /locus_tag="PK856_09545"
     CDS             110..844
                     /locus_tag="PK856_09545"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HRL02650.1"
                     /translation="MKHCVLVVRPETQATSLLNYCQQQGWRAELFSSMEVSLLPHTWD
                     DVQQRLNATDVAFWVSANAIISAYQLRVNPPLHNVCVGPGTLSCFYQYFPQASASAPL
                     DGIDSEAVLRMPLWQDSALRQVTILNGEGGRDWLTNRLLGMGKSVQTISLYQRVEKRI
                     DWNRFRQLSQDHHLAVCVYSSEAVELLFQQVPADLRAKLQSLLYFTIHQRIADELIAQ
                     GAGRILVGKQGHGSVLKLLREHLIST"
     gene            924..2168
                     /locus_tag="PK856_09550"
     CDS             924..2168
                     /locus_tag="PK856_09550"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF016275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HRL02651.1"
                     /translation="MAEQPQEKPEQTPYSSSNEVKPVAAYAQPVVIKQGGGKALGLGA
                     LVLSLIALGASGLLFVQGQNSINNLDTKVNNKIDQAKIGEVDAVSAVKKNQVDQQDLQ
                     NKLQLLQKDNQRLNNQLTQVDQAYQQLVKSRHQWLVDEIEFSLNVATQQLLLTGNKEA
                     TIKSLKSIESRLNQFDKPELVPLKQAISTDLVALSQGTSNVNITSTAVMISSLEGRVD
                     GLKTLLEGVLTKPEATDTASNPQLPWWQNVWNDIKGSFKGLVEVRKLDNKDAMLISPE
                     QSEYLKENIRLHLVSARIALMQQQSELYKSELQAVDTAMRSYYDMNDPLTKAWLADLA
                     QVQQIQLGGNPADGLKNSLAAVQQFQQQNQDQASVFNLPQTPSSASAPQLATPPASTS
                     APQATPAKPKAASEPEVKGGRA"
     gene            2165..>2632
                     /locus_tag="PK856_09555"
     CDS             2165..>2632
                     /locus_tag="PK856_09555"
                     /inference="COORDINATES: protein motif:HMM:NF018875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HRL02652.1"
                     /translation="MKALIWLVVLFAGAAAVAVLTHSYPGNVFIIMGDELRRISLNTF
                     ILSTVIFVVVLYLLMTLLGRVVSIPGGMRRYGKRRRSAKVADALNEAGQAFFEGRFQK
                     AQTEADKVLKNKECGDAAPLALMLAAYSAEQTNDDTAKQGYLQRLAALPESMQL"
BASE COUNT          748 a          542 c          664 g          678 t
ORIGIN      
        1 gtggcatatg cagatggttt aggacgtgcg gtggcgaaaa aattggctga tgatggtgct
       61 cacgccttga tacaggccgt attggcacaa gcgtaaacgg aaatcattca tgaagcattg
      121 cgtattggtg gtaagacccg agacacaagc aacatccttg ttgaattatt gccaacaaca
      181 aggatggaga gctgagttgt tttcaagcat ggaagtcagc ttattgcctc atacttggga
      241 tgatgtgcaa cagcgcttga atgccactga tgtggcgttt tgggtaagcg ccaatgccat
      301 catcagcgcc taccaattgc gggtgaatcc gccattgcac aatgtgtgtg tggggccagg
      361 tacactcagt tgcttttatc agtacttccc gcaagcgagt gccagtgcac cactagatgg
      421 catagacagt gaggcggtat tgcgcatgcc tttgtggcaa gattcggcat tgcgccaagt
      481 caccatttta aatggagaag gtgggcgtga ttggttgacc aatcgtttac tcggtatggg
      541 taaatcggtg caaaccatct cactttatca gcgtgtagaa aaaagaatcg attggaaccg
      601 ctttcgtcag ttgagccaag accatcattt ggccgtttgt gtgtattcaa gtgaggctgt
      661 agaacttttg tttcaacaag tccctgccga tttgcgtgcc aaactccaat ccttgttata
      721 cttcaccatc catcaacgca tagcagatga attgattgca caaggtgcag ggcgcatatt
      781 ggttggaaaa caaggtcatg gaagcgtcct gaagctattg cgagagcatt tgatatcaac
      841 ataacaacat ttgttttcag atttgtgcac ttcggtatta aaaatgcatt catcaaagac
      901 aatgattaac ttaggagggc catatggcag aacaacctca agagaaacct gaacaaacac
      961 cttatagcag ttcaaacgaa gtcaagcctg tggcagctta tgctcaacca gtggtcatta
     1021 agcaaggtgg cggtaaagca ttgggtttgg gggctttggt attgtctttg attgccttag
     1081 gtgcaagtgg tttgttgttt gtacaaggcc aaaacagcat caataatttg gacaccaagg
     1141 ttaacaataa aattgatcag gcgaaaattg gcgaagtcga tgccgtctct gctgtgaaaa
     1201 aaaatcaagt tgatcaacag gatttgcaga ataaattaca gttgttacaa aaagacaatc
     1261 aacgtctgaa caaccaattg acccaagtgg atcaagccta tcaacaattg gtgaagtcac
     1321 gtcaccaatg gttggttgat gaaatcgaat tcagcttgaa tgtggctacc cagcaattgt
     1381 tgttgacagg caataaagaa gccaccatca agtcactgaa aagcatagaa agccgtttaa
     1441 atcaatttga taaaccagaa ttggtaccct taaaacaggc tatcagtacg gatttggtgg
     1501 cattgagcca agggacttcc aatgtcaata ttacttcaac agcggtgatg atcagcagtt
     1561 tggaaggccg agtggatggt ttgaaaacct tattagaagg ggttttgacc aaaccagagg
     1621 cgactgacac tgcgagtaat ccgcaactgc cgtggtggca aaatgtttgg aatgacatca
     1681 aaggcagttt taaaggtttg gtagaagtac gcaaactgga caataaagat gccatgttga
     1741 tttcgcctga acaaagtgaa tacttaaaag aaaacatccg tttgcacttg gtcagtgccc
     1801 gcattgcctt aatgcagcag caaagcgaat tgtacaaaag tgaactacag gccgttgata
     1861 ctgccatgcg cagctattac gacatgaatg acccactgac taaggcatgg ctagctgatt
     1921 tggcgcaagt gcaacagatt cagttgggtg gtaatcctgc cgatggtttg aaaaacagct
     1981 tggcagcggt tcaacagttc caacagcaaa atcaagacca agcaagcgtt ttcaatttgc
     2041 cacaaacacc aagctcggct tctgctcctc aattggctac cccgccagca tcaaccagtg
     2101 ccccacaagc gactcccgct aagccaaaag cagcgtctga gcccgaagtc aaaggagggc
     2161 gcgcatgaag gcattgattt ggttggtggt attgtttgcg ggagcagcgg ctgtcgcggt
     2221 gttaacccat tcttaccctg gaaatgtgtt cattatcatg ggtgatgaat tgcgacgtat
     2281 ttctctcaat acgtttattt tgagcacagt gatatttgtg gttgtgctgt atctgctgat
     2341 gactttgttg gggcgtgtgg tatcgatacc aggtggcatg cgtcgctatg gtaagcgtcg
     2401 ccgcagtgcc aaagtggccg atgctttgaa tgaggcaggt caggcttttt ttgaaggtcg
     2461 ttttcaaaaa gcccaaactg aagcagacaa ggtgttgaaa aacaaagagt gtggtgatgc
     2521 tgcgccattg gccttgatgt tggcggcgta cagtgccgaa caaaccaatg atgacactgc
     2581 caaacaaggt tatttgcagc gtcttgctgc cttgcctgag tccatgcagt ta
//
LOCUS       DAOMVK010000318         2626 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_11613_length_2626_cov_3.024893, whole genome shotgun sequence.
ACCESSION   DAOMVK010000318 DAOMVK010000000
VERSION     DAOMVK010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11613_length_2626_cov_3.024893"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="PK856_09560"
     CDS             complement(<1..781)
                     /locus_tag="PK856_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02653.1"
                     /translation="MGNASAQAVNLYALLGVPADVDGARLRQVIDLHHAAKLLPDAIL
                     DKAERYLLSEGLRGQYNVYLSQISFFPSQLSLAQINLYAHLNVPHHASVAEIQAALNT
                     RLHRIPLQTWLLARKWLLHPRLRERYDGLWAQRLAGGDALASAEDTNVALLPSPTESE
                     TTAEHRTPWLLLTAAALATAVGVVTVERALSRPQPVSVQQTVLPPLLVATPASQAFTQ
                     ITITAVGDTTLGSHLGDTSTGSLPAVWQKQGDAYFMQHMADV"
     gene            1006..1230
                     /locus_tag="PK856_09565"
     CDS             1006..1230
                     /locus_tag="PK856_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02654.1"
                     /translation="MNDKLTPEQLAKIKPIPYSDDDDDKDDDYAQLSHEQLEQINRQN
                     SGNFVEGLVSDAITPDKDKPKPPPLPQDDE"
     gene            1282..1911
                     /locus_tag="PK856_09570"
     CDS             1282..1911
                     /locus_tag="PK856_09570"
                     /inference="COORDINATES: protein motif:HMM:NF014447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HRL02655.1"
                     /translation="MNTHPNIRLFQADLMRAPRDFIAPTITPTMCLEIGAGKGKHAMQ
                     FAAQHPDTHLLAIERTAEKFQAFLKQHQVLSLHNLQPIHADAIAWSVHGLKPQQLAQI
                     FILYPNPEPKNPAQRWLNMPFFEFLLSRLQDGGRITLASNIPDYIAEAALQCQNVWQL
                     PFVQETIPTHFDRTHFETKYLERGEPCSQLIITKPQGYVTRFDAFDKTV"
     gene            complement(1971..2495)
                     /locus_tag="PK856_09575"
     CDS             complement(1971..2495)
                     /locus_tag="PK856_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02656.1"
                     /translation="MFCLQAAAAIATALPKLAQAKPWQAPARLPKAYVDLVRNIDTHM
                     FSADAVLWTACVAAHHNSAKQQELLWDDGVDVATRNRYWLFAENGLGDVYVLDEQGQC
                     YFADHDEGSLHPSFFLPLQLDFSGWLRLADLMGQCDVVLDVQDELDNAQIAAFNTCLA
                     QLHPDLPAHYPYQL"
BASE COUNT          701 a          766 c          599 g          560 t
ORIGIN      
        1 acacatcggc catgtgttgc atgaaatagg catcgccttg tttttgccac accgcaggca
       61 aactgcctgt gctcgtgtca cctaaatggc tgcccaatgt cgtgtcgccg acggcggtga
      121 tggtgatttg ggtaaaggct tgtgatgcag gtgtcgccac taacagcggt ggcaacaccg
      181 tttgctgcac agacacaggc tgaggccgcg acaaggcacg ctctaccgtc accacaccca
      241 cggccgttgc caaggcagcc gccgtcaaca acaaccacgg cgttctatgc tcagcggtgg
      301 tttccgactc agtgggcgaa ggtaacagcg caacattcgt atcttctgcc gatgcaagag
      361 catcaccgcc agccaaacgc tgcgcccaca aaccatcgta gcgctcacgc aaacgcgggt
      421 gcagcagcca cttgcgtgcc agcagccaag tttgcaatgg aatgcggtgt aaacgcgtgt
      481 tcaatgcggc ctgtatttcc gccacactgg cgtgatgagg cacattcaaa tgggcataca
      541 ggttgatttg tgccaaagac aattggctgg ggaaaaacga gatttggctc aaatacacat
      601 tgtattgacc acgcaagccc tcgctaagca aatagcgctc ggctttgtcc aaaatcgcgt
      661 ctggcaagag cttggcggca tgatgcaaat ctatcacctg acgcaagcgc gcgccatcga
      721 catcggctgg cacacccaat aaggcataca aattcaccgc ttgagcagaa gcattcccca
      781 tgatagtttc ttttgatgtg ttttggctga aactgccttc ccttggcagc cataaattgc
      841 gaatgtcatt ttttcactca caaatccatt gtagcgcaaa cgcggtaaaa agaatcgctt
      901 gcgagcccaa gcggccgcac ttaaacttta caggccgctg tttgaaccat acgccgacaa
      961 acacagtctg ttttattctg attttcattt gatgcgaggc aatccatgaa cgacaaatta
     1021 acccccgaac aattggccaa aatcaaaccc atcccgtaca gcgatgacga cgacgacaaa
     1081 gatgatgatt atgcacagct gagccatgag caattggagc aaatcaaccg ccaaaacagc
     1141 ggtaactttg ttgaaggctt ggtcagcgat gccatcacgc ctgataaaga caaacccaaa
     1201 ccaccgccac tgccgcaaga cgacgagtga tgccaacacc acacaacgcc cattctgggc
     1261 gttttgcatt acaatacgcc catgaacacc catccaaaca tccgcctgtt tcaggccgac
     1321 ctcatgcgcg caccgcgtga ttttattgcc cccaccatca ctcctaccat gtgtttggaa
     1381 ataggcgcag gtaaaggcaa acatgccatg caatttgctg cgcaacatcc tgatacccat
     1441 ttgttggcga tagagcgcac ggcggaaaaa ttccaagcct ttctcaaaca acaccaagtg
     1501 ctgtctttgc acaatctgca acccatccat gccgatgcga ttgcttggag tgtgcatggg
     1561 cttaagccac agcaactggc gcaaattttt attctctacc ccaatcccga gcctaaaaat
     1621 cccgcgcagc gctggttgaa catgccgttt tttgaatttt tgctgtcgcg tttacaagac
     1681 ggtggccgca tcaccttggc cagcaacatt cccgattaca tcgccgaagc cgctttgcaa
     1741 tgccaaaacg tatggcagct gccgtttgtg caagaaacca tccccacaca ttttgaccgc
     1801 acccattttg aaaccaagta tctggaacgc ggcgagccct gctcacaact catcatcacc
     1861 aagccgcaag gctatgtcac ccgttttgat gcctttgata aaacggttta aaccaaaaat
     1921 aagcggcctg taagtttaca ggccgcttat tagtcatgca ctgtcataca ttacagctga
     1981 tacggataat gggcaggcaa atcaggatgc aattgcgcca aacaagtgtt gaatgcagcg
     2041 atttgtgcgt tatccaattc gtcttgcaca tccaaaacca catcgcactg ccccatcaaa
     2101 tcggccaaac gcagccagcc agaaaagtcc agctgcaaag gcagaaagaa actggggtgc
     2161 agactgcctt catcgtggtc ggcaaaataa cactgccctt gttcgtccaa cacatacaca
     2221 tcgcccaaac cgttttccgc aaacagccaa tagcggttgc gcgtagccac atccaccccg
     2281 tcgtcccaca acaactcctg ttgtttggcg ctgttgtgat gggcggccac acacgccgtc
     2341 cacaaaaccg catcggcgga aaacatgtgg gtatcgatgt tgcgaaccaa atccacatac
     2401 gctttaggca aacgggcagg cgcttgccag ggcttggctt gcgccaattt gggaagggca
     2461 gtggctatcg ccgctgcggc ctgtaaacaa aacatcttgc cctcctacta atgcgccccg
     2521 ccaccaccgc ctttggcggt aaacggaggc ttggcaaacc acaccaagcc aattaacacg
     2581 atgaacaaaa tcgcccccgc gtaaaacacc tcattcgagg ccatga
//
LOCUS       DAOMVK010000319         2609 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_11692_length_2609_cov_2.013312, whole genome shotgun sequence.
ACCESSION   DAOMVK010000319 DAOMVK010000000
VERSION     DAOMVK010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11692_length_2609_cov_2.013312"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PK856_09580"
     CDS             complement(<1..227)
                     /locus_tag="PK856_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02657.1"
                     /translation="MQRKNSLEVLQILKQQRVLLAISVFTVITVLLLINNYQLLPTGN
                     ETGRVAQLLGIALAMFVGVIAYFNITQSKRK"
     assembly_gap    706..744
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <745..>2609
                     /locus_tag="PK856_09585"
     CDS             <745..>2609
                     /locus_tag="PK856_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02658.1"
                     /translation="LGNLFNTSVDVDAFVPAATGSKYAAVRICSSKTQENLTDSMFLG
                     QPHCIDMRRQLLKQLLPFLNRTGGFEQETPFSGFDISRRYARLLFSQHQDWVWSSIVS
                     SLREANVEAQNIVILIDMADSFASAVWIDCLFGLKERYPLASFYPVFLLPEMENLAIE
                     EAAVIGAAVTELSQLSQGRWTPSDINTGEDYPCGEPLWAVAYLQQCPQVQNDTTYLQL
                     VEAVLILSNVGHNDYGTLRQTLTQEYQKILKPNNAKSAKMLPRFASMLQASLDFDVEA
                     LEGLLAEQCTSKLLASLTNTVAPEQAQPLDIDNMTAMGCVLTQAQLTLDEDSCGEHIG
                     VWSNVDIDWQQHSATLLQQPSSGGDWFSQAEGIASQLRGIYDKQFRKVGAEQFYEASE
                     LRLTQMAQNHVAYVETQMWQAWQQGHSSLSDLQAAVEGIIIHYVQWHQDFLMHQELNI
                     DAIKVCATWWEDLSGQWQRADKKERALLAKEYSVATVADHMSRLFIERCKYKSNLFAE
                     KMILAMQPFLLDLQQSLYQASEAWLLQATQAQKNLNQILDTQQANWLKQVIQAMHCRY
                     LLQPNKASLQTLANHTRNAARDAAPAVHSACVKKLGKQAGFADMLAFIQAGTWQE"
BASE COUNT          715 a          549 c          630 g          676 t
ORIGIN      
        1 tgtttgcgct tggattgggt aatgttgaaa taggcaatca cccccacaaa catggccaaa
       61 gcaatgccca ataattgtgc cacacgacct gtttcattgc ctgttggcag caattggtaa
      121 ttgttgatta acagcagcac ggtgatgacg gtgaataccg aaatggccaa caagacccgt
      181 tgttgtttca agatttgcaa cacctcaagt gagtttttcc gctgcatgct aataccccag
      241 ttatgcgcat cttatataat gagtatatga agattgtaat gatgaaaata acatttcata
      301 ccgcctgatt ttcacaaaaa cacatatgtt ttttacactt agcgggcggt gaaaaagata
      361 tatgtgtgtt tattctgaaa tcattaaagt taaaatttgg ttttttaagt caataaatct
      421 tagtataccg ttgcaatatc cgcaatacat tatttctaaa aaatcctatt tattgaagtg
      481 attacagcga ttttaaaaat caattggagt ggatagcttg tttaaagtag attgcgatta
      541 atgcaactaa ttgtcaactg aattgatact ttaaggtttt ttactttaat ttatttttta
      601 aggtttgaag ttaaagctgg taaattcacc gttccaaata taagtacatt ataaatacaa
      661 gtggttaggc tgtaacacat cagccaatga agcggagagc gcacannnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnncttggg taatttgttc aatacatctg ttgatgttga
      781 tgcctttgtg cccgcagcga ctgggagcaa atatgccgca gtccgaattt gcagcagcaa
      841 aacgcaagaa aacttaaccg acagcatgtt tttgggacaa ccgcattgca tagacatgcg
      901 ccgccagttg ttaaaacaac tgttgccgtt tctcaatcgc acgggtggat ttgaacaaga
      961 aaccccattt tcaggctttg acatttcgcg cagatatgcg cgtttgttgt tttcgcaaca
     1021 ccaagattgg gtgtggagca gcatcgtcag cagtttgcgc gaagccaatg tggaagccca
     1081 aaacatcgtc atcttaatcg acatggccga ttcgtttgcc agtgcggtgt ggatagactg
     1141 cttgtttgga ttaaaagagc gttatccgct ggccagtttt taccctgtgt ttttattgcc
     1201 cgaaatggaa aatttggcaa ttgaagaagc agcggtcatt ggtgcggcgg tgacggagct
     1261 gagccaattg agccaaggcc gttggacgcc gagcgacatc aatacgggtg aagactaccc
     1321 atgtggcgag cctttgtggg cggtggcgta tttacagcaa tgcccgcaag tgcaaaacga
     1381 caccacgtat ttgcaattgg tggaagcggt gttgattttg tcgaatgtgg gccacaatga
     1441 ttacggcact ttgcgccaaa cgctgaccca agagtatcaa aagattttaa agcccaacaa
     1501 tgccaaaagt gccaaaatgt tgccgcgctt tgcgtctatg ctgcaagcgt cgctggactt
     1561 tgatgtggag gccttggaag gcttgttggc cgagcaatgc acttcaaaac tgttggcgtc
     1621 tttaaccaat actgtcgcac ctgagcaagc gcagcctttg gacatagaca acatgaccgc
     1681 gatgggttgt gtgttgaccc aagcacaatt gactttggat gaagacagtt gtggcgagca
     1741 cattggggtg tggagcaatg ttgacatcga ttggcagcaa cacagtgcca ctttgttaca
     1801 acagcccagc agtggtggcg attggttcag ccaagccgag ggcatcgcca gccaattgcg
     1861 cggcatttac gacaagcagt ttcgcaaagt gggggctgag cagttttacg aagccagtga
     1921 gttgcgtttg acccaaatgg cacaaaacca tgtggcctat gtggaaacgc aaatgtggca
     1981 agcttggcag caaggccaca gcagcttgag tgatttacag gccgctgttg agggcatcat
     2041 cattcattat gtgcagtggc accaagattt cttgatgcac caagagctca acattgatgc
     2101 catcaaagtg tgcgcgacat ggtgggaaga tttgtcaggg caatggcaac gcgcggacaa
     2161 aaaagagcgt gcattactgg ccaaagaata cagtgtggcc accgtggccg atcacatgag
     2221 ccgcttgttc attgagcgct gtaagtacaa atccaatttg tttgccgaaa aaatgatttt
     2281 ggcgatgcag ccatttttat tggatttgca acagagtttg taccaagctt ctgaagcgtg
     2341 gctcttacag gccacccaag cccaaaagaa tctgaaccaa atcttagaca cccaacaagc
     2401 caactggctc aaacaagtga tacaggctat gcattgccgt tatttgttgc agccgaataa
     2461 ggccagtttg cagactttgg ccaaccacac ccgcaatgcc gcccgtgatg ccgcgcctgc
     2521 tgtacacagc gcctgcgtga aaaaactggg caaacaagcg ggttttgctg acatgcttgc
     2581 cttcatacag gccggtactt ggcaagaag
//
LOCUS       DAOMVK010000320         2607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_11704_length_2607_cov_2.705329, whole genome shotgun sequence.
ACCESSION   DAOMVK010000320 DAOMVK010000000
VERSION     DAOMVK010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11704_length_2607_cov_2.705329"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(286..774)
                     /locus_tag="PK856_09590"
     CDS             complement(286..774)
                     /locus_tag="PK856_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02659.1"
                     /translation="MKLKMVLSTALATSLVLCMSHVQARRIAPAEPIPVAFQGLWVAG
                     DDAAAQKQACKILSQTPQSASAASFSYIKVSEYSLFVHTPSDQTTYDGASRSEPWHKQ
                     TATRLEGTVYPSSRYLGRGEFNSNTTPLELEWRINSRGYLVAKGFKPRKYYRCYSRNT
                     AG"
     gene            complement(787..>2607)
                     /locus_tag="PK856_09595"
     CDS             complement(787..>2607)
                     /locus_tag="PK856_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HRL02660.1"
                     /translation="KEAATKIKTQTAALHKLGSKRDFDQMARVYEQDTDYEIPHVIFL
                     IDLKDNKRVDYINTPKYSLHETYLAQAKGFKPNDAQLKSYYYSPDRHYIFGTLSWQNT
                     SREYVYEFWEGDKITPSLLKTADQAIKASFFAPVRYKTNSTWQEDVATQTKLPFVTQE
                     SLIKDFPYLSLHQGKAVGTLRVIEKEDDLYDVGSDDIIILKEVPLAMPPVAAVISEKP
                     STALSHVNVLARGWGIPNVYLKDAEKILAPYIGKRISLNAAENQYQVTLAKDGSNTVS
                     NKPNAIKLPSINTSDLKLRDLRSLRQSDHVYCGSKAANLGQINANIKVANVPDGFCIP
                     FGYYQQFMQQLGIDSAYLQHMEASFKGNNRARRAGLLALQEKIQAAPVPQAWQNAWGA
                     QWQNQLRSQGVFVRSSSNSEDLPNFSGAGLYTTVPNVKSKQALSEAVKQSWASVFNYS
                     AYEARRIAGLPHDSVKMSTFVQQGINADLSGVLVTLDPYDASRKNVSYIAAKRGVGIR
                     VVEGKRLAEQLVYNHRVGSIQLISSSNETTALQLDDKGGVKEVPIDTGKRVMTDAQVK
                     TLANTGSQIKAIFGNKQQDIEWAFTNDRLVILQARPYLTR"
BASE COUNT          559 a          616 c          681 g          751 t
ORIGIN      
        1 ccattgtgta caggccgctt ggcgcaaggt gtcatctgct tggttaatcc atgtcccttg
       61 cagcggcgta gcaatgacgt ccagaacgcg gatggcgtgg gctgaagtac tgagcagcag
      121 caataaaaac aagggcagca aacgttccat aaggtgtctt tacattttct caatcaagaa
      181 caataagttg ggtcgtgggt gtgcgtcttg ttgctaacca ttcgtggcac cacagcgttt
      241 acaggccgct tatttctctg tttttggtgt actgaaatgg tgtgtttacc cagcggtgtt
      301 gcggctgtaa caacgataat attttcttgg cttaaagccc ttggcgacaa ggtaaccgcg
      361 ggaattgatg cgccattcca gttctagagg ggttgtattg gaattgaatt cgcctcgccc
      421 caaatacctg ctgctcgggt atacagtgcc ttccaaacgg gtggcagttt gcttatgcca
      481 aggctcactc cgacttgccc catcgtatgt ggtttggtcg ctgggcgtat gcacaaacaa
      541 cgagtattca ctcactttga tgtatgaaaa agaggcggca gaagctgatt gaggcgtttg
      601 tgacaaaatc ttacaggctt gcttctgtgc tgctgcatcg tctcctgcaa cccacagccc
      661 ttggaatgcc actggaatcg gctctgcagg tgcaatgcgt ctggcttgca catgcgacat
      721 gcacaatacc aagctggttg ccaatgcggt tgaaagtacc attttgagtt tcatgatgcg
      781 ctcctgttaa cgggtcaaat acggccgtgc ttgcaaaatc accaagcggt cattcgtaaa
      841 cgcccattca atgtcttgtt gtttgttgcc aaagattgct ttgatttgtg aacctgtgtt
      901 ggccaaggtt ttgacttgtg catctgtcat cacccgtttg cctgtgtcta tgggcacttc
      961 tttcacgccg cctttgtcgt ccaattgtaa ggctgtggtt tcattcgacg acgaaatcaa
     1021 ttgaatcgag cccacacggt ggttatacac caattgctcg gccaagcgct tgccttcgac
     1081 cacacgtatg cccacaccgc gtttggctgc gatgtagctg acgttcttgc gcgaggcatc
     1141 gtaagggtct agcgtgacca acacgcccga caaatcggca ttgatgcctt gttgcacaaa
     1201 cgtgctcatt ttcacgctgt cgtgtggtaa tcccgcgatg cgtctggctt cataagcgct
     1261 gtaattgaat accgacgccc acgattgttt caccgcctca ctcaaggcct gtttgctttt
     1321 cacattcggc acggtggtgt agaggcctgc accactgaaa ttgggcaagt cttctgaatt
     1381 ggacgagctg cgcacaaaca cgccttggct gcgcaattgg ttttgccatt gtgcgcccca
     1441 tgcgttttgc caagcttgcg gcacaggtgc ggcttggatt ttttcttgca aggccaataa
     1501 gcctgcgcgg cgggcgcggt tattgccttt gaatgaggct tccatgtgtt gcaaataggc
     1561 gctgtctatg cccaattgct gcatgaattg ttggtaataa ccaaacggta tgcaaaagcc
     1621 atcgggcaca ttggcgactt tgatgttggc gttgatttgg cccaaattgg ctgctttgct
     1681 gccacaatac acatgatctg attggcgcaa gctgcgtaaa tcgcgcagct ttaaatcgct
     1741 ggtgttgatg gagggcagct tgatggcatt gggtttgttg ctgaccgtat tgctgccatc
     1801 ttttgccaaa gtcacttggt attggttttc agcggcattg aggctgatgc gcttgcctat
     1861 gtagggtgcg aggatttttt ctgcgtcttt caaatacaca ttgggtatgc cccagccgcg
     1921 cgccaacaca ttcacatgtg agagggcggt agatggtttt tcactgatga cggcagccac
     1981 cggcggcatg gcaagcggta cttcttttaa gatgatgatg tcgtctgaac ccacatcgta
     2041 caaatcgtct tctttttcaa tcacgcgcaa agtgcccact gccttgcctt ggtgcaaaga
     2101 caaatacgga aaatccttaa tcagactttc ttgggtgaca aacggcagct tggtttgcgt
     2161 ggccacgtct tcttgccaag tggaattggt tttgtagcgt actggtgcga aaaagctggc
     2221 tttaatcgct tggtcggcgg ttttgagcaa ggaaggggtg attttgtcgc cttcccaaaa
     2281 ttcatacaca tactcgcgac tggtgttttg ccaagacaga gtgccgaaaa tatagtggcg
     2341 gtcggggctg taataatagc ttttcaattg tgcatcattg ggcttaaagc ctttggcctg
     2401 cgccaaatac gtttcgtgca ggctgtattt gggggtgttg atgtaatcaa cacgcttgtt
     2461 gtctttcaaa tcaatcagaa aaatcacatg tggaatttcg taatcagtgt cttgctcata
     2521 tacccgcgcc atttggtcaa aatcccgttt gctgcccagt ttgtgtaagg cggcggtttg
     2581 ggttttaatt ttggtggccg cctcttt
//
LOCUS       DAOMVK010000321         2606 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_11709_length_2606_cov_3.670325, whole genome shotgun sequence.
ACCESSION   DAOMVK010000321 DAOMVK010000000
VERSION     DAOMVK010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11709_length_2606_cov_3.670325"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..634
                     /locus_tag="PK856_09600"
     CDS             131..634
                     /locus_tag="PK856_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HRL02661.1"
                     /translation="MLDVNLIDPVAFAKSALLQEDSIGLTQLDERVAQHEFLAHADTT
                     LHYRLKGGVDKLQRPFLDLSLQGEFQLACQRCLAAMPFVLDETVRIVLYRDEAVLDEA
                     MDSDPDLEGMLAESELSVWSLLEDQIIMALPYAPRHDDCGNDDLERVNTDKPNPFAQL
                     AKLKSNQ"
     gene            667..846
                     /gene="rpmF"
                     /locus_tag="PK856_09605"
     CDS             667..846
                     /gene="rpmF"
                     /locus_tag="PK856_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRL02662.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDSLTAPATAVDATTGEVHLRHHISP
                     NGMYRGRKVIKSNDE"
     gene            1022..2059
                     /gene="plsX"
                     /locus_tag="PK856_09610"
     CDS             1022..2059
                     /gene="plsX"
                     /locus_tag="PK856_09610"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003785217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HRL02663.1"
                     /translation="MITIAVDAMGGDIGLSVTVPASVAFLERQSDVKLLMVGDEAKLL
                     AALKSVHAPMNRISVIHASEVVDMDEDPRNALRHKKDSSMRVAINQVKEGLAEAAVSA
                     GNTGALMATAKFVLKTIEGIERPAIAKFLPTSNGGLTCVLDLGANIDSTPEHLLQFAI
                     MGAQLVQAVNPQVKSPRVGLLNIGSEEIKGNSTVKEAYQLLQNSELNFIGNVEGDDIF
                     TGEVEVIVADGFVGNVALKTIEGTVKMMGQIIKSEFNSSALSKAQGLIAKPVLAQVKK
                     RIDPRRFNGAIFLGLRGVVVKSHGGTDAVGFNYALQEAYHEAKADILTSIQRGVCAHL
                     AAADSEQNTDH"
     gene            2154..>2606
                     /locus_tag="PK856_09615"
     CDS             2154..>2606
                     /locus_tag="PK856_09615"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="FabH; beta-ketoacyl-acyl carrier protein synthase
                     III; catalyzes the condensation of acetyl-CoA with
                     malonyl-ACP to initiate cycles of fatty acid elongation;
                     differs from 3-oxoacyl-(acyl carrier protein) synthase I
                     and II in that it utilizes CoA thioesters as primers
                     rather than acyl-ACPs; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HRL02664.1"
                     /translation="MTYAKILGTGSYLPANRVSNDDLAQKVDTSDEWIVTRTGIRHRH
                     VAAENELTSDLAVQAALQAIDSSGLDKNDIDLIIVATTTPDKTFPSTACIVQQKLGMP
                     GVAAFDVQAVCAGFMYALTTAHAYILAGLAKNALVIGAETFTRLLDWND"
BASE COUNT          672 a          585 c          686 g          663 t
ORIGIN      
        1 aagggcagat tcaattgttg caattgcatt tggcgaaagc gtgaactcga tgccaaaatc
       61 agtgctggga gtgtcatgga gaaatttgtc attgacagtt agaatcaaac aattatatca
      121 tccttcgttt atgttagacg ttaatttgat tgatccggtc gcttttgcaa aaagcgcgtt
      181 gcttcaagaa gatagcattg gcttgacgca attggacgag cgcgtggcgc agcatgagtt
      241 tttggcccat gccgatacaa cacttcatta ccgcttaaaa ggcggtgtgg acaagttgca
      301 acgccccttt cttgatttgt cattacaggg cgagtttcag cttgcatgtc agcgctgttt
      361 agcagcgatg ccgttcgtgt tggacgagac agttcgcatc gtgttgtacc gcgatgaggc
      421 tgtgttggat gaggccatgg acagtgatcc cgatttagag ggaatgctgg ctgagtctga
      481 gctttctgtg tggtcgctct tggaagatca aatcattatg gctttgcctt atgccccacg
      541 ccacgacgat tgcggcaacg atgatttaga gcgggtcaat accgataagc ctaatccttt
      601 tgcccagttg gccaagctga aaagcaacca ataaacgtat ttattttctc ttatggagtt
      661 tgactcatgg cagttcaaca aaacaaaaag tccccttcta aacgtggtat gcaccgttca
      721 cacgacagct tgactgcacc tgcaacagct gttgatgcaa ctactggcga agtacatttg
      781 cgtcaccaca tctctcctaa cggtatgtac cgtggtcgta aagtaatcaa atcaaacgac
      841 gaataagtct gtttgattgc gaaagccaac accagcacac gcttgatgtt ggcttttttg
      901 cgtttgggta tcacgtgaag cgggtttgac ttaattttgc gtgactttca cacgcagtgg
      961 tttgtggtgc atttcttcgc atgataagct tgctgtgttt agaaaatgat tgagtagaaa
     1021 aatgataaca attgcagtgg atgcgatggg cggtgacata ggcttaagcg ttaccgtacc
     1081 ggcttccgtg gcttttttag agcgccaaag cgacgtcaaa ttgctgatgg tgggtgatga
     1141 agccaagtta ctggccgcat tgaaaagcgt gcacgcgcct atgaatcgca tcagtgtgat
     1201 acatgcttct gaagtggtgg acatggatga agaccctcgc aatgccttgc gccataagaa
     1261 ggactcttct atgcgcgtgg cgattaacca agtaaaagaa ggcttggctg aggcggcggt
     1321 gtctgcaggc aacacaggcg cattgatggc cactgcaaaa ttcgttttga aaaccattga
     1381 aggcattgag cgccctgcaa ttgccaaatt tttgcccaca tctaacggcg gtttgacttg
     1441 cgtgttggac ttgggtgcca acatcgacag cacacctgaa catttgttgc agttcgccat
     1501 catgggcgca caattggtgc aggcggtcaa tccgcaagtg aaatcaccac gcgtgggttt
     1561 gctcaacatc ggttcggaag aaatcaaagg caacagcacg gtcaaagagg cctaccaact
     1621 cttgcaaaac agtgagttga atttcattgg caatgtcgaa ggcgatgata ttttcacggg
     1681 tgaagtagaa gtaatcgtgg ccgatggctt cgttggcaat gtggccttga aaacgattga
     1741 gggcacagtg aaaatgatgg gccaaatcat caagagtgaa ttcaacagca gcgctttgag
     1801 caaggctcaa ggtttgattg ccaaacctgt gttggcacaa gtgaagaaac gcatagaccc
     1861 acgccgcttc aatggcgcca ttttcttggg tttgcgtggc gtggtggtga aaagccatgg
     1921 cggcaccgat gccgtgggct ttaactatgc cttgcaagaa gcgtatcacg aagccaaagc
     1981 cgacatattg accagcatac agcgcggggt gtgcgcccat ttggctgccg ccgattctga
     2041 gcaaaacacc gaccattaga caatattcag gcgtttgtta tgcagccgtt ggcaggcaaa
     2101 cgtcagagtc agtgacatca gcaggacaca gagaaagtcg agaacgtatt accatgacgt
     2161 atgcaaaaat tttgggcacg ggcagctatc tgcctgctaa ccgtgtcagc aatgatgatt
     2221 tggcgcaaaa agtagacact tctgatgaat ggatcgtcac gcgcacaggc atacgccacc
     2281 gccatgtggc tgctgaaaac gaattgacca gcgatttggc cgtacaggcc gcattgcaag
     2341 ccatagacag ctctggcttg gataagaacg acatcgactt aatcatcgtg gccaccacta
     2401 cacctgacaa aaccttccca tctactgcct gtattgtgca acaaaaattg ggcatgcctg
     2461 gtgtggccgc gtttgatgtg caagccgtgt gtgcaggctt tatgtatgcc ttgaccacag
     2521 cgcatgccta tattttggcg ggtttggcca agaatgcctt ggtcattggt gcggaaacct
     2581 ttacccgttt gcttgactgg aacgac
//
LOCUS       DAOMVK010000322         2605 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_11714_length_2605_cov_3.963922, whole genome shotgun sequence.
ACCESSION   DAOMVK010000322 DAOMVK010000000
VERSION     DAOMVK010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11714_length_2605_cov_3.963922"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PK856_09620"
     CDS             <1..468
                     /locus_tag="PK856_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HRL02665.1"
                     /translation="ARFKELADKKREIFDEDLHALVSDELVSITPDRYKYLSLKIQTE
                     TGELPQAEIVISEQGQEYRATSQGSGPVDAVFKAIESVAPSHAQLQLYSVNAITEGTE
                     SQGEVTVRLSKDGFIVNGQGADTDVIVASAKAYISALNKLQKPDERIKAQGMI"
     gene            591..1178
                     /locus_tag="PK856_09625"
     CDS             591..1178
                     /locus_tag="PK856_09625"
                     /inference="COORDINATES: protein motif:HMM:NF017747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HRL02666.1"
                     /translation="MTSLHTFDLQTLPAMAWKNGGGVTREIVREPAGASLDDFDWRVS
                     VADIAAAGPFSRFLGIDRHLLLLEGNGVNLTSDEAGLNVNLLADDTVLAFSGDVDMDS
                     QLLGGSVRDFNVMTKRGVWQAQVSVWTEAGSVQVQAGLVLQTQGQACCSFAEGDATFK
                     TGQGGWWQEGVQNLHWQPLSDDAKAIVVVLQSVNA"
     gene            complement(1350..1871)
                     /locus_tag="PK856_09630"
     CDS             complement(1350..1871)
                     /locus_tag="PK856_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02667.1"
                     /translation="MSIKSFFAKLQRKTSSQPELSPNELLEQTRIRIAQADTDFDYLN
                     QAQHFQQSNGEFWFHYSQVLRPLPESQLMDLRPNGPIAAAVYAAEKCLVAAPYSKGAN
                     AEYILALNMLTHGLSSLLNADLMHPILAQRNETEIWFKHTFNTLKTACQKACQQFPDD
                     EWFQTELDEVMQF"
     gene            complement(2037..>2605)
                     /locus_tag="PK856_09635"
     CDS             complement(2037..>2605)
                     /locus_tag="PK856_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRL02668.1"
                     /translation="VRSGANTGDVALVGGAGPIGLLLGAILKAKGLTVIMTELSAKRK
                     EKALEAGVADYVLDPSQVDVLAEVMKITADKGADVSFECSSVNAVLDTLVAATKPTGV
                     VVIVSIWSHPATVNVHNVVMKELDMRGTIAYCNDHAETIKLVEEGKINLEPFITQRIK
                     LDDLISQGFETLIHNNESAVKIIVNPNL"
BASE COUNT          659 a          550 c          730 g          666 t
ORIGIN      
        1 gcccgcttta aagaattggc cgataaaaag cgtgaaatct tcgatgaaga cttgcatgct
       61 ttggtgtctg acgaattggt cagcatcacc cccgaccgtt acaaatacct gtcattgaaa
      121 atccaaactg agaccggtga gctgccacaa gcagaaatcg tcatcagtga acaaggtcaa
      181 gaataccgcg ctaccagcca gggttcaggt ccagtggatg ccgtgttcaa agcaattgaa
      241 tctgtggcgc ctagccatgc acaactgcaa ttgtattccg tgaatgccat taccgaaggc
      301 accgagagcc aaggcgaagt gacagtgcgt ttgtctaaag acggttttat cgtcaacggt
      361 cagggtgcag acaccgatgt gattgtggcc agtgccaaag cgtatatttc ggcattgaac
      421 aaattgcaaa aacccgatga acgcatcaaa gcccaaggca tgatttaagc gtttgtcgca
      481 gagacaataa aatagaagag gacaagcggc ctgtaaaagt ttacaggccg cttttatatt
      541 ggtctgtatg gtttctacag tacactgcac agacaggatg aggagaaaac atgacaagcc
      601 tacacacctt tgatttgcaa accctgcctg cgatggcgtg gaaaaacggc ggcggcgtga
      661 cgcgtgaaat tgtgcgtgag cctgcagggg cgagtttgga tgattttgac tggcgggtga
      721 gcgtggccga tattgcggcg gcagggccgt tttcgcgctt tttgggcata gaccgccatt
      781 tgctgttgct agaaggcaat ggcgtaaatt tgaccagtga cgaagcgggc ctgaatgtga
      841 atttgttggc agatgacacg gtgttggctt tcagtggtga tgtggacatg gatagccagt
      901 tacttggcgg ctcggtgcgc gacttcaatg tgatgaccaa gcgcggtgtg tggcaagcgc
      961 aggtaagcgt gtggactgaa gcgggcagtg ttcaggtaca agctggattg gttttgcaaa
     1021 cacaagggca agcgtgctgt tcgtttgcag agggcgatgc cacatttaaa acagggcaag
     1081 gtggttggtg gcaagagggc gtgcaaaact tgcattggca gccgctgagt gatgatgcta
     1141 aggccatagt ggtggtgttg caaagcgtga atgcttgaac acaaagtatg gcacagtgtc
     1201 gattacaaag tttacaaagt tgcgaaaaat accattgcct gttttgatgc agaatacaat
     1261 tcaagctgcc tgcaagttta caggcagcta ggactggtgg tagcaccatg gctttggttt
     1321 agtgcagaca tcaaagtgac aggatagttt taaaattgca tcacttcatc cagttcggtt
     1381 tgaaaccatt catcatctgg aaattgttgg caggcttttt ggcaagctgt cttgagggtg
     1441 ttgaatgtgt gcttaaacca aatttctgtt tcgttgcgtt gggctaaaat ggggtgcatt
     1501 aaatcggcat tcagcagaga gctcagtcca tgggtgagca tgttcagcgc aagaatgtat
     1561 tcggcattgg caccttttga ataaggtgcc gccacaaggc atttttcagc cgcataaacc
     1621 gctgcggcga tggggccatt tgggcgcaaa tccatgagtt gagactcagg tagggggcgc
     1681 agcacttggc tgtagtgaaa ccaaaattcg ccattgcttt gttgaaaatg ttgggcttgg
     1741 ttcaggtaat caaaatcagt atcggcttgt gcaatacgga tgcgggtttg ttcgagcaat
     1801 tcgtttgggg acagttcagg ttgtgatgag gtttttcgtt gaagtttggc gaagaaggac
     1861 ttgatagaca taaggtggct ttgagtttgt aagaaataac atgaataagc aagctcaata
     1921 ccaaggtaag gcaagtagga aaggcggggg cagtggtttg aggtataaat ttgcctatgt
     1981 aaaaagcggc ctgtaaactt acaggccgct tgtttgctta tcggagattc aactcattac
     2041 agatttgggt tcacaatgat tttcacagcc gattcattgt tgtgtatcag ggtttcaaag
     2101 ccttgtgaaa tcaaatcatc gagcttgatg cgttgggtaa tgaaaggctc aagattgatt
     2161 ttgccttctt ccaccagctt gatggtttct gcatggtcgt tgcaataagc gatggtgccg
     2221 cgcatgtcca attctttcat caccacgttg tgcacattca ctgtggcagg gtggctccaa
     2281 atcgacacga tcaccaccac gcctgtcggt ttggtagcgg cgaccaaggt gtccaatacc
     2341 gcattgacgc tgctgcattc aaacgaaaca tccgcgcctt tgtcggccgt gattttcatc
     2401 acttcggcca acacatcgac ttgtgatggg tcgagcacgt aatcggccac gcccgcttcc
     2461 aaggcttttt ctttgcgctt ggcactgagc tcggtcatga tgacggtgag gcctttggcc
     2521 ttgagtatcg cgccgagcaa caaaccgatg gggcctgcgc cgccgaccaa agccacatcg
     2581 cctgtatttg cgccactgcg cacaa
//
LOCUS       DAOMVK010000323         2596 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_11774_length_2596_cov_3.326643, whole genome shotgun sequence.
ACCESSION   DAOMVK010000323 DAOMVK010000000
VERSION     DAOMVK010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11774_length_2596_cov_3.326643"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="PK856_09640"
     CDS             complement(<1..746)
                     /locus_tag="PK856_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HRL02669.1"
                     /translation="MNLKLQQYSLIIAVGLAVTAPALADSYDPYEPYNRFMYRINDGI
                     DRTVLVPVSRGYQRVTPKPVQSGVTNFFDNLRDVVSIGSNLLRLDIEKASTDLVRVGI
                     NTTFGLGGLINIADAAQMPNNKNSLGDTFATWGWKNSNYFVYPLAGPSTVRDAVGDTV
                     LRVYSLEGAIVESEGLRWGLGALKGIDKRAQLLVYTDGLDMAAVDRYAYTRDVFMNMR
                     ATQVGGTPPSAVYDDTNIDDLVPSEADTPAP"
     gene            complement(743..1015)
                     /locus_tag="PK856_09645"
     CDS             complement(743..1015)
                     /locus_tag="PK856_09645"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRL02670.1"
                     /translation="MQLTHQDDVLFLKGDATVSSITREDLRLLEQACKQSTQIVDLSG
                     LDKVDSTCVALLIAIKRSLGNQAWQLRSVPAAVSALLELYELEWLA"
     gene            complement(1019..1609)
                     /locus_tag="PK856_09650"
     CDS             complement(1019..1609)
                     /locus_tag="PK856_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRL02671.1"
                     /translation="MKKFAFMNALAIGVLSIGIAVASPQQAVTQLRTNADQVLNILSQ
                     ANGSNNAAVRRQAENYAIPYFDFERMTALAVGAPWKQATPAQKQALTQEFKTLLIRTY
                     SGTMLKFKGAKVQVLGNPIVNNGGKEVVVKTKITAQGKPVNMDYTMYQSGSKYRVYNV
                     AVEGASLVTVYRSQFNETIRKSGIDGLISQLKQKNG"
     gene            complement(1675..2163)
                     /gene="mlaD"
                     /locus_tag="PK856_09655"
     CDS             complement(1675..2163)
                     /gene="mlaD"
                     /locus_tag="PK856_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HRL02672.1"
                     /translation="MKRSVLELWVGIFVVLGVAALLFLSFRVASGSSDADGGQAYTVT
                     ASFNDIGGLKVKAPVKAAGVVVGRVTSIVLDPKDYRAKVSIQLQKQYQFSTDTSAQIL
                     TSGILGEQYIGLMQGAEEELLANGSNIDLTSDALVLEQLIGKFMTSFAEKNAGDSEAA
                     AK"
     gene            complement(2169..>2596)
                     /locus_tag="PK856_09660"
     CDS             complement(2169..>2596)
                     /locus_tag="PK856_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRL02673.1"
                     /translation="IGLMKTTEQLDAMDVMAVNPVARVVAPRFWAGVISMPFLAAIFN
                     VCGIFGAYFIGVKHLGLDGGIFWSQMQSNIDWSYDVLNGIIKSVVFGIAVTLIAVYNG
                     FSSRATPEGILRATTQTVVASALAILGLDFVLTAFMFTE"
BASE COUNT          680 a          626 c          603 g          687 t
ORIGIN      
        1 ggtgcaggcg tgtctgcttc agagggtacc aaatcatcaa tattggtgtc atcgtatacg
       61 gcgctaggag gggttccgcc cacttgggtg gcccgcatgt tcatgaacac atcgcgggta
      121 taagcatagc ggtctacggc tgccatgtcc aagccatcgg tgtaaaccaa cagttgcgca
      181 cgtttgtcta tgccttttaa agcccccaag ccccaacgca gaccctcgga ttcaactatg
      241 gcgccttcaa gcgagtacac ccgcaatacg gtatcaccga cggcatcacg aacagtagac
      301 gggcctgcca acgggtagac aaaataattg ctgtttttcc aaccccaagt ggcgaaggta
      361 tcgcccaaag agtttttgtt attgggcatt tgcgccgcat cggcaatgtt gattaagcca
      421 cccaaaccaa aagtggtgtt gatgccaaca cgcaccaagt cggtactggc tttttcaatg
      481 tccaaacgca acaaattgct gcctatgctg accacatcgc gcaaattgtc gaaaaagtta
      541 gttacgcctg attgaacagg tttgggcgtg actctttggt agccacgtga cacaggaacc
      601 agcacagtgc ggtctatgcc atcgttaatg cggtacataa aacgattgta tggttcatac
      661 ggatcgtagc tgtcggccaa tgcaggagcg gtgactgcca agcctacggc gatgatgagc
      721 gaatattgtt gtaattttaa gttcatgcta accactccag ttcatacaat tctaataagg
      781 cgctgactgc agcaggcaca gagcgcaatt gccacgcttg attccccaat gagcgtttga
      841 tggcaatgag caaagccaca caagtggaat cgactttgtc caatccagac aaatcgacaa
      901 tttgtgtgct ttgtttacac gcttgttcta gcaagcgtaa atcctcgcgg gtgatgctgg
      961 atacggtggc atcacccttg aggaataaca catcatcttg gtgtgttaat tgcatgtttt
     1021 aaccgttttt ttgtttcaat tggctaatca agccatcaat gcctgatttg cgtatggttt
     1081 cattaaattg gctgcggtag accgtaacca agctggcacc ttcaacagcc acattgtaca
     1141 cgcggtattt gctgccgctt tggtacatgg tgtagtccat gttgacaggc ttgccttgag
     1201 cagtgatttt ggttttaaca accacttctt tgccaccgtt gttcacgata gggttgccca
     1261 atacttgcac tttggcgcct ttgaatttta acatggtgcc actgtaggta cgaatcaaca
     1321 aggttttaaa ttcttgggtt aaagcttgtt tttgtgcagg agtggcttgt ttccacggtg
     1381 cacctacggc caaagcagtc atgcgctcaa aatcgaaata agggatggca tagttttctg
     1441 cttggcgacg cacagcggca ttgttactgc cattggcttg gctcaagata ttcaaaactt
     1501 ggtcggcatt ggtgcgcaat tgggtaacgg cttgttgtgg gctggcgact gcaatgccta
     1561 tgctcaagac accgattgcc aaggcattca tgaatgcaaa tttcttcatt gttgttcctt
     1621 aagtttaaat actgtttttg ttgttaaaag cggcctgtaa ccgctttaaa agtgttattt
     1681 cgcggctgct tcagagtcac ccgcgttttt ttccgcaaag ctggtcatga atttgcctat
     1741 gagttgttcc aataccaagg catcgctggt taaatcgatg ttgctgccat tggccaaaag
     1801 ttcttcttct gcaccttgca tcaaaccgat gtattgctca cccaaaatgc ctgaagtgag
     1861 aatttgggct gaagtatcgg tgctgaattg gtattgcttt tgcaattgta tcgacacttt
     1921 ggcgcggtaa tctttcgggt ccaagacgat gctggtcaca cgacccacca ccacacctgc
     1981 ggctttgact ggtgctttga ctttcaggcc gcctatgtcg ttaaacgatg cggtaacggt
     2041 ataagcttgg ccgccatcgg catcgctgct gccactggca acgcgaaacg ataaaaacaa
     2101 taaagcggcg acacccaata cgacgaaaat gccaacccac aattctaaga cgctgcgttt
     2161 cataatcatt actcagtaaa cataaatgcg gttaatacga aatccaaacc caaaatcgcc
     2221 aaggcactgg caaccacggt ttgggtggtg gcacgtaaaa tgccttcagg tgtggcacgg
     2281 ctagaaaaac cgttgtacac agcaatcaaa gtcactgcaa tgccaaatac caccgatttg
     2341 atgatgccgt ttaacacatc ataagaccaa tcaatgttgc tttgcatttg tgaccaaaaa
     2401 ataccgccat ccaaacccaa gtgtttgact ccaatgaaat aagcaccaaa aataccacaa
     2461 acattgaata ttgctgctaa gaaaggcatg gaaatcacac ctgcccaaaa gcgtggggca
     2521 acgacacggg caactgggtt gaccgccatc acatccatgg catccaattg ctcggtggtt
     2581 ttcatcaagc caattt
//
LOCUS       DAOMVK010000324         2593 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_11794_length_2593_cov_3.293144, whole genome shotgun sequence.
ACCESSION   DAOMVK010000324 DAOMVK010000000
VERSION     DAOMVK010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11794_length_2593_cov_3.293144"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PK856_09665"
     CDS             <1..489
                     /locus_tag="PK856_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HRL02674.1"
                     /translation="NSELSLAIMAEVADVFYIGGTKNGALLGEAIVIVNPDLQADFRY
                     GMKQHGALLAKGRILGLQFVGLFAENVFFECAAHAHEQSMVLAQGLRDLGCGFLTEPQ
                     SNQIFPILPNKVIAALQEKFAFYHWQNINEQDSAVRLVTSWATEPENVNEFLRVTTEL
                     LR"
     gene            1135..1863
                     /locus_tag="PK856_09670"
     CDS             1135..1863
                     /locus_tag="PK856_09670"
                     /inference="COORDINATES: protein motif:HMM:NF027468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4850 domain-containing protein"
                     /protein_id="HRL02675.1"
                     /translation="MRHTLLIAAILSGISGLAAAQTYQVSPPKFAQEQAVRQAQNQWH
                     AIGNAYLANGKTVPAYAISVSNPVEEGLLQDNTACTRKSCDLNVTLNDGQASQLQALR
                     INGVGWVLAPRAWKNIEAAVGVNGSQSVLLQSPDGSESMTYYNSGACVGCAYSAASPF
                     FPAALKLAQENEFGYDEPNAAINIVRPQANQALFSYTVKGQHTTHGKAVFDQNTDIPY
                     QNMRVTLKPSHKDLANIILNAGVR"
     gene            complement(1987..>2593)
                     /locus_tag="PK856_09675"
     CDS             complement(1987..>2593)
                     /locus_tag="PK856_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02676.1"
                     /translation="MSAGPSREVTLDGKTQLYALALGNAYDFANKQPNQSHADSGIAA
                     LYVYQKSGNGWQLIHRGKPDHIGAYGEAPRGKDWTFHQFGPKTHGYLAMHSDMHHGYA
                     GSHYIILTPWKQGVANSWLGANYNTEGASETGKTYADLEATFYINTQARPTAGMYPLV
                     FKVRNNSNGKKQPVKTYTVPFNASKGAYVAPKNYPLNNIDY"
BASE COUNT          663 a          608 c          652 g          670 t
ORIGIN      
        1 aacagcgaat tgagtttggc catcatggcg gaagtggccg atgtgtttta cattggcggc
       61 accaaaaatg gcgctttatt gggcgaagca attgtgattg tgaaccctga tttacaggcc
      121 gactttcgct atggcatgaa gcaacatggc gcattgctgg ccaaagggcg catcttgggt
      181 ttgcaatttg tgggcttgtt tgctgagaac gtgttttttg aatgtgccgc gcatgcacac
      241 gagcaatcca tggttttggc acaaggtttg cgcgatttgg gctgcggctt tttgaccgag
      301 ccgcaatcca accaaatttt ccccattttg cccaacaagg taattgcagc attgcaagaa
      361 aaatttgcct tctaccattg gcaaaacatc aatgagcaag acagcgccgt gcgcttggtc
      421 acttcttggg caacagagcc tgaaaatgtt aatgagtttt tacgtgtgac tacagaatta
      481 ttacgttaat aacctaaagc ccaacttttc tcaatgctat attcaataag cattatcttg
      541 cagctggatt tttcatcgct ttgatggcat cgatttgaaa aaaccagctg ccattcatac
      601 ggcatgtaag gcaacgcgaa tgacctgttt ggggtggtgc aaccgcagat tcattgtgac
      661 taaaaccatg ccaagcgatt gtgccagtag ccgtactcaa aaacacacat tttaaccgca
      721 ccattgcgcg atgctggaat gggtcttgca tagatgcaca tgcggatgtg ggctttgctt
      781 gtggcacgat tcctcaccat cttgccaccg tagcaaagcc acagccgccg ttgaaagata
      841 catggtttca agcccaaccc gcaacctcaa atgggttttc atactggctt tgaaatcatg
      901 gacatgcaaa cgcagttgag tgaaatgaat gcgagcaggc agggcaaaaa tgcgacctgt
      961 aagacatggt ccacggcata caatgtggcc aacaaaccaa ctgtgtttgc ttgtgattgc
     1021 agcatttcat cgctgcccaa atacaataaa aatgcctctt gcttggatgg tcatgcgtgt
     1081 catggctggc acaccgtcac ttgctaagag gaactgaaga attaaggagt tatcatgcgt
     1141 catactttgc tcattgctgc cattctgagc ggcatcagtg gtttggcagc tgcccaaacc
     1201 tatcaagtgt cgccacccaa gtttgcgcaa gagcaagctg tgcgtcaggc acaaaaccaa
     1261 tggcatgcca tcggcaatgc ttatttggca aacggtaaaa ccgttcccgc ttacgccatc
     1321 agcgtgagca atcctgttga agaagggttg ttacaagaca atacggcttg cacacgtaaa
     1381 agctgtgatt tgaacgtaac acttaatgat ggccaagcga gccaactgca ggctttgcgc
     1441 atcaatggcg tgggttgggt cttggcacca cgggcttgga aaaatatcga agcagcagtg
     1501 ggcgtgaatg gttcacaatc ggttttattg caaagcccag acggcagtga gtccatgact
     1561 tattacaaca gtggcgcttg cgtgggctgt gcttacagtg cggcatcgcc atttttcccc
     1621 gccgcgttga aattggcgca ggaaaatgaa tttggctacg atgaacccaa tgccgccata
     1681 aacattgtgc gcccacaagc gaatcaggcc ttgttcagct ataccgtcaa aggccagcac
     1741 accactcatg gcaaggcagt gtttgaccaa aacaccgaca ttccttatca aaacatgcgg
     1801 gtgaccttaa agcccagcca caaagatttg gcaaacatta ttttgaatgc aggtgtcaga
     1861 taagcgacaa cctatatcag aataaaatat cagaataaaa ctaaaacaca agcggcctgt
     1921 aagtttttac aggccgcttt ttgatgatgg ctgcttcatt gtatgcatag gcaatgagcc
     1981 ttaggttcag taatcgatgt tgttcaaagg atagtttttc ggggcaacat acgcgccttt
     2041 gctggcattg aacggcacgg tataggtttt cacaggctgt tttttgccat tgctgttgtt
     2101 gcgcactttg aacaccaagg gatacatgcc tgcggtaggc ctggcttggg tgttgatgta
     2161 aaacgtggcc tccaaatcgg cataggtttt acccgtttcc gaagcccctt cggtgttgta
     2221 attcgcgccc aaccagctgt tggccacgcc ttgtttccat ggggtcaaaa tgatgtaatg
     2281 gctgcccgca tagccatggt gcatgtcgct gtgcatggcc aaatagccat gggttttggg
     2341 gccaaattgg tgaaacgtcc aatccttacc gcgtggcgct tcgccatatg cgccaatgtg
     2401 gtcgggcttg ccgcggtgga ttaattgcca gccattgcct gatttttggt acacatacaa
     2461 ggcggcaatg ccgctgtcag catgggattg gttgggctgc ttattggcaa aatcataggc
     2521 attgcccaag gccaaagcat acaactgggt tttgccatcg agtgtcactt cgcggctggg
     2581 gcctgctgac atg
//
LOCUS       DAOMVK010000325         2585 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_11847_length_2585_cov_3.019368, whole genome shotgun sequence.
ACCESSION   DAOMVK010000325 DAOMVK010000000
VERSION     DAOMVK010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11847_length_2585_cov_3.019368"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /gene="glmM"
                     /locus_tag="PK856_09680"
     CDS             complement(<1..774)
                     /gene="glmM"
                     /locus_tag="PK856_09680"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244655.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRL02677.1"
                     /translation="MARKYFGTDGVRGLVGQDPITPEFVLKLAFAAGKALVAREQGHR
                     PMVLIGKDTRISGYMLEAALQAGFTAAGVDVHLTGPLPTPGIAYLTRAMRASAGVMIS
                     ASHNAYHDNGIKFFAQGGIKLNDDMELAIEALIDQPMQTMPSDDLGRSKRISGADDRY
                     IEFCKSTFPSEQDLHGLKIVVDCANGAGYHVAPKVFHELGAHVISIGCEPNGFNININ
                     VGATHTETLQQAVLANKADYGVALDGDGDRLMMVDRTGRV"
     gene            complement(855..1697)
                     /gene="folP"
                     /locus_tag="PK856_09685"
     CDS             complement(855..1697)
                     /gene="folP"
                     /locus_tag="PK856_09685"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRL02678.1"
                     /translation="MRWQCGRFELNLDHLKVMGIVNVTPDSFSDGGQYSSNLDSVLSH
                     AQQLLDEGADILDVGGESTRPGSDYVSPEAEWKRVAPVIEALYQWNVPLTLDTRRTYV
                     MQQALQNGWVDAINDVAALSDEGAVAALAPYQNIGVCLMHMQGIPETMQHNPQYSDVT
                     AEVVQYLQGRVDACLQAGFAPERLLLDPGFGFGKTLQHNIDLMRDLQTWISESNYPVL
                     IGVSRKTMLGQITGINIPEERMIASVAAALASIARGAHIVRVHDVKETVQAVKIWQEL
                     GVWA"
     gene            complement(1758..>2585)
                     /locus_tag="PK856_09690"
     CDS             complement(1758..>2585)
                     /locus_tag="PK856_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HRL02679.1"
                     /translation="GPERRSMVMHEDEKRATAYHESGHAVVASLLPNTDPVHKVTIVP
                     RGRALGVTWQLPERDRISLYKDQMLNNIAVLFGGRIAEDIFLGRVSTGASNDFERATG
                     IARDMVTRYGMSDKMGVMVYAENEGEVFLGRSVTNTKNISEDTQKQVDAEVRRILDEQ
                     YALAYKLIDENRAKMETMTNALMQWETIDADQVKEIMAGKEPSPPKDYSHNVRSEAEI
                     AAAATADVAADNTDHASVETSKAEPESVVVDADKPSADLAADSIVGDKDKDQNSPKQ"
BASE COUNT          672 a          707 c          543 g          663 t
ORIGIN      
        1 cacgcggcct gtacggtcta ccatcatcaa acggtcgcca tcgccatcca aggcaacgcc
       61 gtagtcggct ttattggcca acaccgcctg ttgtaaggtt tcggtatggg tagcgccgac
      121 gtttatattg atgttaaaac cattgggttc acagcctatg ctgatgacgt gtgcgcccaa
      181 ttcatgaaac actttgggtg caacgtgata gcctgcgccg ttggcacaat caaccacgat
      241 tttcaaacca tgcaaatctt gctcgcttgg aaaagtggat ttgcaaaact caatatagcg
      301 atcatccgca cccgaaatgc gcttgctgcg acccaaatca tcagatggca tggtttgcat
      361 gggttgatcg attaaggctt ctatcgccaa ttccatgtca tcattgagct tgatgccgcc
      421 ttgggcaaaa aacttaatgc cgttatcgtg gtaagcattg tgagaagctg aaatcatcac
      481 ccctgcagat gcgcgcatcg cacgggtcaa atacgcaata ccaggagtcg gcagcgggcc
      541 agttaagtgt acatccacac cagcagcggt aaaaccggcc tgtaaagccg cttccaacat
      601 atagccagaa atgcgtgtgt ctttgcctat cagtaccatg ggtctgtggc cttgctctcg
      661 cgccaccaag gctttgcctg ctgcaaacgc caatttcaac acaaactcag gcgtaatcgg
      721 atcttgccct accaaaccac gcacaccatc ggtaccaaaa tactttctag ccatctactt
      781 ctcatcctga ttattctaat taagaaacaa ttgtaaatcg acttacccac tattaacagc
      841 aaaacttcga gtaactatgc ccaaacaccc agttcctgcc aaatttttac ggcttgcacc
      901 gtttctttaa catcgtgcac acgcacaatg tgcgcccccc gcgcaatcga agcaagcgcc
      961 gcagccacag aggcaatcat gcgctcttca gggatgttta tgcctgtgat ttggcccaac
     1021 atggttttgc gcgacacgcc aattaataca ggataattac tttccgatat ccaagtttgt
     1081 aaatcccgca tcaaatcaat gttgtgctgc aaggttttgc caaaaccaaa accagggtcg
     1141 agcaacaaac gctcaggtgc aaaaccggcc tgtaaacacg catccacacg tccttgtaag
     1201 tattgcacca cttctgcggt cacatctgag tattgtggat tgtgctgcat agtctcaggt
     1261 ataccttgca tgtgcatcaa gcacacacct atgttttgat aaggtgctaa agccgctacc
     1321 gctccctcat cacttaaggc cgccacatca ttgatggcgt ccacccagcc attttgtaaa
     1381 gcttgctgca tcacataagt gcgacgggtg tccaaggtta agggaacatt ccattggtag
     1441 agcgcttcaa tcacaggcgc aacacgcttc cactcagctt caggagacac ataatcactg
     1501 cctgggcggg tagattcacc gcccacatcc aagatatctg caccttcatc cagcaattgc
     1561 tgtgcatggc tcagcacact gtctaaatta cttgaatatt gtccaccatc cgaaaaagag
     1621 tcgggagtca cattaacaat acccatgact tttaggtgat ccaaattcaa ctcaaaacgg
     1681 ccacactgcc aacgcatact tcatcctttt gacaaataaa aacggcagcc ctagctgccg
     1741 tttggttcaa attgacacta ctgcttgggt gagttttgat ctttgtcttt gtcacccacg
     1801 atgctgtcag cagccaaatc agcactaggc ttgtctgcat caactaccac agattcaggc
     1861 tcggctttag atgtctcaac agaagcatgg tctgtattat cagcagcaac atctgctgta
     1921 gcagctgcag caatttcggc ctcagaacgc acattgtgac tgtaatcttt aggtgggctt
     1981 ggctctttgc ccgccatgat ttctttcact tggtcagcat caatggtttc ccattgcatc
     2041 aaagcattgg tcatggtttc cattttcgca cggttttcat caatgagttt gtatgccaaa
     2101 gcatattgct catccaagat acggcgcact tctgcatcca cttgcttttg tgtgtcttca
     2161 gagatgtttt tggtgttggt taccgaacgg cccaagaaca cttcaccctc gttttcggca
     2221 tacaccatca cacccatttt gtctgacata ccgtaacggg tcaccatgtc acgggcgatg
     2281 ccagtggcac gctcaaagtc attggaagca cctgtagaca cacgacctaa gaaaatgtct
     2341 tcagcaatac ggccaccgaa caataccgca atattgttga gcatttgatc tttgtacaaa
     2401 ctgatgcgat cgcgctctgg taattgccaa gtcacaccca aagcacggcc acgaggcaca
     2461 atggtcactt tgtgtactgg gtcagtattc ggcaacaaac ttgccaccac agcatggcca
     2521 gactcatggt aagccgttgc acgtttttca tcttcgtgca tcaccataga gcgacgctct
     2581 gggcc
//
LOCUS       DAOMVK010000326         2574 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_11900_length_2574_cov_3.250893, whole genome shotgun sequence.
ACCESSION   DAOMVK010000326 DAOMVK010000000
VERSION     DAOMVK010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11900_length_2574_cov_3.250893"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            360..1355
                     /locus_tag="PK856_09695"
     CDS             360..1355
                     /locus_tag="PK856_09695"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019389856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HRL02680.1"
                     /translation="MNITVLGAGAWGTALAIHFAKQKHQVRLWARDTEHVLQMKQSRC
                     NERYLDGFQFPAELSLETDLQAAIDATELILIATPVAGLRPTLEKLAGNIVNTTPIMT
                     ACKGFEVSTGKLPHQVVADVLPEQQHIVLLSGPSFAQELAEQLPCAVTLAAENNVWIE
                     QLCRDLNSSVLRLYASTDLIGVAVGGALKNVMAIATGLCDGLHYGMNARAALMTRGLA
                     EITRLAQHLGAHEHTLMGLSGLGDLILTCTGGLSRNRQVGLKLAENKDLDTILAELGH
                     VAEGVYTVNEAHRLSQQLQVKMPITETLYALFQGQLNSTQVAQALMMRNPTSESN"
     gene            1506..>2574
                     /locus_tag="PK856_09700"
     CDS             1506..>2574
                     /locus_tag="PK856_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HRL02681.1"
                     /translation="MNTLNIGKLTLSLMVGLALTACGGQKQGDNAAAASGNNEIVIGF
                     AAPLTGPQSHYGEEYKNGAQLAIDEANAQKLTINGKPATFKLMAEDDAADPKQATQVA
                     ERFIDAKVAGVIGHFNSGTAIPASKIYEDNQIPNIAMATSPTLSAQGFKYVFRSMTSD
                     SQQGTVLGKYAVEKLGYKKIAIIDDRTAYGQGLADEFEKSAKAAGATILPRQFTNDKA
                     SDFQSILTSFKTVQPDVIFYGGADTQSAPMIKQIRRLGINAPFISGEMTKTPTFLKVA
                     GPDAEGVFTSLAGFPLEKMPKGKEYETKYKTKYNQDVATYSPYGYDATWAMITAMKQA
                     NSSKAADYLAKLQAMDLDGVTS"
BASE COUNT          737 a          630 c          548 g          659 t
ORIGIN      
        1 tttctttacg cgctgttcga tattttccat gttacttcct ttaccttgat cttaaataag
       61 ggtttgtttt agatagaatt tatttgcaaa tcatcaaaat tcagtgctta ttgtactgaa
      121 tttaaataga gttttccatc catcaaatga agctattgta cgcatattcc cagcattttt
      181 gccagtactc tgtgcatgca acaagccgat tttcgattta gcgggcatat tagcacaagg
      241 tcaaatgctc tctcaaccaa caaactagct atattattca aaattcaagt ttttgcactc
      301 ttgcctcagt gttattctcg tggcaaggtt aaagacatgg aacacaaaag gttaaacaaa
      361 tgaatatcac tgttttgggc gctggcgcat ggggaactgc tttggccata cattttgcca
      421 aacaaaaaca tcaagtgcgc ttatgggcgc gcgataccga acatgtgttg caaatgaaac
      481 aatcgcgttg caatgaacgt tatttagatg gctttcaatt cccagctgag ttatcactgg
      541 aaacggattt acaggccgct attgatgcca ctgaattaat cctcattgcc acaccagtag
      601 caggtttgcg ccctactctg gaaaaattgg caggcaatat tgtcaatacc acccccatca
      661 tgacggcttg caaaggcttt gaagtttcaa ctggcaaact tcctcatcaa gtggtcgcag
      721 atgttttgcc tgagcagcaa cacatcgtct tattatctgg ccccagtttc gctcaagagc
      781 tggcagaaca actcccatgt gcagtgactc ttgccgcaga aaacaatgta tggatagaac
      841 aattgtgtcg tgatttaaac agctcagtgc tgcgcctgta tgcttcaacc gatttaatcg
      901 gtgtggccgt tggtggtgct ttgaaaaacg tcatggccat cgccactggc ctgtgtgatg
      961 gattgcacta tggtatgaat gcccgagccg cactcatgac tcgtgggttg gctgaaatca
     1021 cccgtttggc ccaacacttg ggtgcacatg aacacacttt aatgggcttg tctggcttgg
     1081 gggacttgat tttaacctgt acaggtggct tgtcacgcaa tcgtcaggtc ggtttgaaac
     1141 tggcagaaaa caaagacctc gataccattt tggcagaatt gggccatgtg gccgaaggtg
     1201 tgtacaccgt caatgaagcc catcgcctat cccaacagct acaagtcaaa atgccgataa
     1261 ccgaaacttt atatgcctta tttcaaggcc aactcaacag cactcaagtg gcacaagcct
     1321 tgatgatgcg caaccccact tctgaaagca attaagcgga ttttagatat taaaattgaa
     1381 gttatttgat tagagtgaaa cctaccaatt tgtcacccca tactctcaat attggttcca
     1441 agctgctaaa gattgcttta ctatgcagga cttttcattt tgcattcagt ctttaggaga
     1501 aactcatgaa cacattgaac ataggcaagc tgactttgag cttgatggta ggtttggctc
     1561 taaccgcatg tggcggccaa aaacaaggtg acaatgccgc tgccgcttca ggcaataacg
     1621 aaattgtcat tggctttgcc gcccctttaa caggcccaca atcccattac ggtgaagaat
     1681 acaaaaacgg tgcacaattg gccatagacg aagccaatgc ccaaaaactc accatcaatg
     1741 gcaaacccgc cacgtttaaa ttgatggcag aagacgacgc agccgatccc aaacaagcca
     1801 ctcaagtggc tgaacgcttt atcgatgcca aagtggctgg tgtgattggt cactttaact
     1861 caggcacggc gattcctgcg tctaagattt acgaagacaa tcaaatcccg aatatcgcca
     1921 tggcaaccag ccctactttg agcgcacaag gctttaaata cgtattccgt tccatgacgt
     1981 ctgacagcca acaaggcaca gttttgggca aatatgcagt cgaaaaattg ggctataaaa
     2041 aaattgccat cattgacgac cgcaccgctt acggccaagg cttggcagat gagtttgaaa
     2101 aatcagccaa agccgcaggc gccaccatct tgccgcgtca attcaccaac gacaaagcct
     2161 cagatttcca atcaatcctg accagcttca aaaccgtgca acctgatgtg attttctacg
     2221 gcggcgccga cacgcaatct gctccgatga tcaagcaaat tcgccgcttg ggcattaacg
     2281 cgccattcat ctcaggcgaa atgaccaaaa ccccaacttt cctgaaagtg gctggcccag
     2341 atgcagaagg cgtgttcacc tcattggcag gtttcccttt ggagaaaatg cctaaaggca
     2401 aagaatacga aaccaaatac aaaaccaaat acaaccaaga cgtggccact tattcaccat
     2461 atggttacga tgcaacttgg gcgatgatta ccgccatgaa gcaagccaac tcgtctaaag
     2521 ccgccgacta cttggccaaa ttgcaagcca tggacttgga tggcgtgacc agcc
//
LOCUS       DAOMVK010000327         2573 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_11911_length_2573_cov_3.167990, whole genome shotgun sequence.
ACCESSION   DAOMVK010000327 DAOMVK010000000
VERSION     DAOMVK010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11911_length_2573_cov_3.167990"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..970
                     /locus_tag="PK856_09705"
     CDS             41..970
                     /locus_tag="PK856_09705"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HRL02682.1"
                     /translation="MVTISARQIQLSHALGLGPMWIGRAAQIVNCDTAPKLAEATPAL
                     PRNTAVSNPVAHIAAAPAPPNTHNTPKPSVPSTASNDSAAANDAASVQHKVSKRAAAL
                     LAHIAQAQQYKPKQDSAQVVAAEHTVVAADCSQDSLLSLQTKITQCTACDLHGERRQA
                     FAAQVRPNCRLIVVLPHPSVQDDAAGELLSGEAGELWLKILKAMRLQADEVYVTNAIK
                     CAPNLSLVPKQHHAAACIGYLQRELQLLPAVPVLLLAENQRSLLKKLEQQVGSERLFR
                     IPHPSKMQRNPDTKREAWEILQTLMPLLPAATQ"
     gene            complement(1029..1493)
                     /locus_tag="PK856_09710"
     CDS             complement(1029..1493)
                     /locus_tag="PK856_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007341665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HRL02683.1"
                     /translation="MTTSTKPYLVRALYEWCVDNAFTPHIAVWVNNYTQVPRQFVQDE
                     QIVLNIGPTACKGLTIDNEWVHFEARFGGKAEDIWIPVGHVVSIFARENSSGMGFEVE
                     AYEPDEAEAAATEAESVLHMVKAEELKANKQDEDDDKTPPPEGSKSFLKIVK"
     gene            complement(1504..2109)
                     /locus_tag="PK856_09715"
     CDS             complement(1504..2109)
                     /locus_tag="PK856_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HRL02684.1"
                     /translation="MMTLYSGITCPFSQRCRIVLFEKGMDFEIKDVDIFNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPADPVQRARGRLFLYRLERELF
                     AHVQVLENAASTKKEQERAREALTEGLTLIAPVFAKQKFVLGEEFSMIDVSLAPLLWR
                     LDHYGIKLGKSAAPLLKYAERLFQRDAFIEALTPAEKAMRR"
BASE COUNT          666 a          669 c          596 g          642 t
ORIGIN      
        1 atgtgtgtga tgaggggcgc gaagacgcgc tgatactaga atggtcacga taagtgcacg
       61 tcaaatacag ttaagccacg cattgggttt gggtccgatg tggataggcc gtgctgcgca
      121 aatcgtcaac tgcgatactg cgcccaaact ggccgaagcc acacccgcct taccgcgcaa
      181 tactgcggtc tctaaccctg tggcgcacat tgctgccgct cctgcccccc ctaacaccca
      241 caatacaccc aaaccctctg tgcccagcac cgcaagcaat gacagtgccg cggccaatga
      301 tgcggcaagc gtgcaacaca aggtttccaa acgtgccgcg gctttacttg cccacattgc
      361 ccaagcgcaa caatacaaac ccaaacaaga cagtgcccaa gtagtcgctg ccgagcacac
      421 ggttgtggca gcagattgct cacaagacag cttgctcagt ttgcaaacca aaatcaccca
      481 gtgcaccgcc tgtgatttgc atggcgaacg ccgtcaggct tttgcggccc aagtgcggcc
      541 aaactgccgc ttgattgtgg ttttgcccca cccctcggtg caagacgatg ccgcgggtga
      601 gttgctcagt ggtgaggcgg gcgagttgtg gctgaaaatt ttgaaagcga tgaggttaca
      661 ggccgatgag gtgtatgtga ccaatgcgat taaatgtgca ccgaatctca gcttggtgcc
      721 caagcaacac catgcggctg cttgcatcgg ttatttgcag cgtgaattgc aattgttgcc
      781 tgccgtgcct gtgttgctgc tcgcggaaaa ccagcgcagc ttgctgaaaa aactcgagca
      841 acaagtgggc agcgaacgct tgttccgcat tccccaccca tccaaaatgc agcgcaatcc
      901 cgacaccaag cgtgaggctt gggaaatctt acaaacactg atgcctctgt tgcccgctgc
      961 cacgcaataa atccatgcaa atacagttgt tcaaatgttt acaggccgct tgatagcggc
     1021 ctgtaaactt atttcacgat tttcaagaaa gacttgctgc cctcgggcgg cggtgtcttg
     1081 tcatcatcct cgtcttgctt gttggctttg agctcttcgg cttttaccat gtgcaataca
     1141 gattcggcct cagttgcggc agcttctgct tcatctggct cataagcctc tacttcaaaa
     1201 cccatgccac tgctgttttc acgggcaaaa atgctcacca catggccaac aggtatccaa
     1261 atgtcttcag ccttgcctcc gaagcgggct tcaaaatgca cccattcgtt gtctatggtc
     1321 aagcctttgc aggcagttgg gcctatgttc aacacgattt gctcgtcttg cacgaattgg
     1381 cgcggcactt gggtgtaatt gttcacccac acggcaatgt gtggggtaaa cgcattgtcg
     1441 acacaccatt catacagcgc acgcaccaaa tacggcttgg tagaagtcgt catgtccgtc
     1501 ctcttagcga cgcatcgctt tttcagcagg cgtcaatgct tcaatgaagg catcgcgttg
     1561 gaacaagcgt tcggcgtatt tgagcaaagg tgcagccgat ttgcccaatt tgatgccgta
     1621 atggtctaag cgccacaaca gcggagccaa agacacatcg atcattgaaa actcttcacc
     1681 caacacgaat ttttgtttgg caaacacagg ggcaatcaaa gtcaaacctt cggtcaaggc
     1741 ttcacgcgcg cgctcttgtt cttttttggt gctggcggcg ttttccaaca cttgcacatg
     1801 agcgaacaat tcgcgttcca aacggtataa gaacaaacgg ccacgggcgc gttgcactgg
     1861 atcggctggc atcaattgtg gatgaggaaa acgctcgtcg atgtattcat tgatgatgtt
     1921 agactcgtgc aaaaccaaat cgcgctctac caaaacaggc acttggttgt aagggttcat
     1981 cactgccaag tcttcaggct tgttgaaaat gtccacgtct ttgatttcaa aatccatgcc
     2041 tttttcgaac aatacgatgc ggcaacgttg gctgaatgga caggtaatcc ctgaatacaa
     2101 agtcatcata ataaagcatt cctcaaaggt taatcttttg tattataagc aatttttaca
     2161 aaaatagcga acttaatagc ctaagacatt gaaagtatta acaaaataag caaaacctga
     2221 agttttgctt attgcgattg caattcaaat gaaaacagca tttaagcggc ctgtaagctg
     2281 tgcatctatt gcttactttg aagaaatggc tcaagtgctt atttttaagc cctcaaatcg
     2341 tttgccatca atgctttaat acaggtacaa aaaatgagca catgtgaatc ttgtttggca
     2401 atgatgtgtt tacctctaag cactctgccc atttttggca aggcaataaa atcagtgaaa
     2461 accatcatcc ccacaagcac atgtgcccat tgctagggtt gtgagcccat catgtgtacc
     2521 gcttaatgca cgtctttcca gtattctttc ttcaagaaat aggccaaagg caa
//
LOCUS       DAOMVK010000328         2569 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_11934_length_2569_cov_3.087112, whole genome shotgun sequence.
ACCESSION   DAOMVK010000328 DAOMVK010000000
VERSION     DAOMVK010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11934_length_2569_cov_3.087112"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..849
                     /locus_tag="PK856_09720"
     CDS             112..849
                     /locus_tag="PK856_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRL02685.1"
                     /translation="MKKTALISSLSVATALLVSACQSSSSVDVRTGTGSQYKNTGPAP
                     AGYHRVQRGENLYRIGLRYNQTVGTLTRWNGLRDASQIEVGQLIRVSRSATGNVASNA
                     SSGNKTSNSKPTSAWSAPSSSVNVGNVRLSRPVSSSVLANFNGGSNKGVDFGGSTGDS
                     VKAAGAGKVVYVGNGLRGYGNVVMVQHSQSLLTVYAHNDQIKVSEGEQVKQGQQLATM
                     GSSGTDRVKLHFEVRQNGKAVNPNLYW"
     gene            1190..>2569
                     /locus_tag="PK856_09725"
     CDS             1190..>2569
                     /locus_tag="PK856_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRL02686.1"
                     /translation="MKKNNRKATSRQDAAQYVAPTMAAFLLTMPFASTAWAAPAVEAD
                     TELPSIEVTATPSSPMQTKESSSNKFTAPLRDTPKTVQVITREVLDQRGVNSLEEALR
                     STPGITFGAGEGGNPTGDQPYIRGINSQQSISIDGLRDIAAGSRETFNVEQVEVIKGA
                     DSAYNGRGGAGGSIIIDTKKPQNTDFTHMDLGVGTDKYVRGSVDTNWSTDNGMAFRFN
                     AMGQKNDVAGREGPENKHWGFAPSVAFGLDTDTRLTLAYYHYQTDDIPDGGVPFFWPK
                     AKPTSGTHYYRPTYGNNRENWYGMYNRDYRKEKTDQGTITFEHDFNEDMKLRNVFRAA
                     RSSQDYVWTQPDDSKFNVENGMVWRRGNARVSDTTTYQNVTDLTGKFDTGALTHSYNV
                     GLELAYEKSDVQSAAVQKGDSKCTNGVGAAGGYMCTDLNNPTPNDPWLYEVVLPEATK
                     YRTKTTSVYA"
BASE COUNT          728 a          594 c          637 g          610 t
ORIGIN      
        1 acccaaactt ggcaacgggt tcagtaaaca gatgcaaaaa taagtaaggc cacctcagta
       61 ggtggccttt ttgtttgccc gcaacgcaag gcagacggta ttatcggcct catgaaaaaa
      121 actgccttaa tctccagcct gtctgttgca actgccttgt tggtcagtgc gtgtcaaagt
      181 tcgtcttctg tggatgtgcg cacgggaacg ggttcacaat acaagaacac agggcctgct
      241 cccgcaggtt atcaccgtgt acaacggggt gaaaatttat accgcattgg tttgcgctat
      301 aaccagaccg tgggcacttt gacgcgttgg aatggtttgc gggatgctag ccaaattgag
      361 gtaggtcagt taatccgcgt gagtcgcagc gcaactggca atgttgccag caatgcttcc
      421 tcaggcaaca aaacatccaa cagcaaaccc acaagtgcat ggtcggcccc aagttcgtcc
      481 gtcaatgtgg gcaatgtgcg tttgagtcgg cctgtaagca gcagtgttct ggccaatttt
      541 aatggcggca gcaataaagg cgtggacttt gggggcagca caggcgacag tgtgaaagca
      601 gcaggagcag gtaaggtggt gtatgtgggc aatggtttgc gtggctatgg caatgtggtg
      661 atggtgcagc acagccaaag tctgctgacg gtgtacgcgc acaacgacca aatcaaagtc
      721 agcgaaggtg aacaagtcaa gcaaggccag caattggcca ccatgggctc atcgggcaca
      781 gacagagtga aattgcattt tgaggtacgt caaaacggca aagcagtgaa tccaaatctg
      841 tattggtaaa ccaatcatgc tttcaaacaa accttacagg ccgcttaata cataagcggc
      901 ctaaattttt agtgatattc aacacaaaat tcaattttga tttaaaaaat caaacacggc
      961 taaatacgta atttaatcaa aatgatagct attatcttta tcattttatt gattgaaatg
     1021 tgcggatcgt gcttaaaaat actgttcata atctttactt acttttatac tgcgaacggt
     1081 aagaagaatg attcctattt gtttagagaa atattctcaa taactatggc gccatgttgg
     1141 taaaacatgg cacagcacac cgttttgtct tgaaagtcac acaaccctta tgaaaaaaaa
     1201 caatcgcaaa gcaacgtctc gccaagacgc agctcagtat gttgcgccaa ccatggctgc
     1261 ttttttattg actatgcctt ttgcaagcac ggcatgggcc gcgcctgcgg ttgaggccga
     1321 taccgagttg cctagcatcg aagtgactgc tacaccaagc agtccgatgc aaacgaaaga
     1381 gtcgtcttct aataaattca ccgcaccttt gcgtgatacg cccaaaaccg tgcaagtgat
     1441 cacgcgtgaa gtgttggatc aacgtggtgt caacagcttg gaggaggctt tgcgttccac
     1501 ccctggcatc acttttggtg cgggtgaggg cggcaatcca acaggtgatc aaccgtatat
     1561 tcgcggcatc aactcacaac aaagcatttc gatagatggt ttgcgcgaca ttgccgcagg
     1621 ttcacgtgaa acctttaacg tggaacaagt ggaagtgatt aaaggcgcag actcagccta
     1681 caatggccgc ggtggcgcgg gtggcagcat catcatagac accaaaaaac cacaaaacac
     1741 cgatttcact cacatggatt tgggcgtggg cacggacaaa tacgtgcgtg gcagcgtaga
     1801 caccaactgg tctaccgaca atggcatggc cttccgcttt aatgccatgg gtcaaaaaaa
     1861 tgacgtggct ggtcgtgaag gtcctgaaaa caaacattgg ggcttcgcac cttctgtggc
     1921 ctttggtttg gacaccgata cccgtttgac tttggcttat taccactacc aaacagacga
     1981 tattcctgat ggtggcgtgc ctttcttttg gcccaaagcc aaacccactt caggtaccca
     2041 ttactatcgc ccaacttacg gtaacaaccg cgaaaactgg tacggtatgt acaaccgcga
     2101 ttaccgcaaa gaaaaaaccg accaaggcac catcacattt gagcacgatt tcaatgaaga
     2161 catgaaattg cgcaatgttt tccgtgcggc acgctctagc caagattatg tgtggacaca
     2221 gcctgacgac agtaaattta atgtagaaaa tggcatggta tggcgtcgtg gtaatgcgcg
     2281 tgtgagtgat accactactt accaaaacgt aaccgatttg acgggcaagt ttgacacggg
     2341 tgcgttgacg cacagctata acgtgggttt ggagctggct tatgaaaaat cggatgtgca
     2401 aagtgccgcg gttcaaaaag gtgatagtaa atgcaccaat ggtgtcggag cagctggcgg
     2461 ctacatgtgt accgatttga acaatccaac accgaacgat ccatggttat atgaggtggt
     2521 gttgccagaa gccaccaaat accgcaccaa aaccacttct gtgtatgcg
//
LOCUS       DAOMVK010000329         2569 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_11936_length_2569_cov_2.257757, whole genome shotgun sequence.
ACCESSION   DAOMVK010000329 DAOMVK010000000
VERSION     DAOMVK010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11936_length_2569_cov_2.257757"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..179)
                     /locus_tag="PK856_09730"
     CDS             complement(15..179)
                     /locus_tag="PK856_09730"
                     /inference="COORDINATES: protein motif:HMM:NF018891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HRL02687.1"
                     /translation="MMKMYALSLGVGVLVGVLYYLLNVKSPAPPFVALLGLLGMVVGE
                     KLIPLLKTLM"
     assembly_gap    263..272
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<273..2065)
                     /locus_tag="PK856_09735"
     CDS             complement(<273..2065)
                     /locus_tag="PK856_09735"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRL02688.1"
                     /translation="MTHAQLILKSGKITTLNKKQTEVQAIAIADGKVLRTGSNEDVMK
                     LAQADTKIVDLNGRRVIPGLNDSHQHLIRGGLNYNMELRWEGVPSLSDAMRLLKEQAD
                     NTPAPQWVRVIGGWSEFQFAEKRLPTLDEINKAAPDTPVFVLHLYGSAMLNHAALKAL
                     GFDKNTPNPPGGQIQRDAHGEPTGLLIATPSAMILYSTLGMAPKLELEDQVNSTRHFM
                     RELNRLGITSVIDAGGGGQNYPDDYAVIQRLHDDNQLNIRIAYNLFAQKAGSELQSYQ
                     EWTQMTFPGDGDAYLQMNGAGENLTWSAGDFENFLEPRPDLLPKMEDELERIVELLAE
                     KKWPFRLHATYDESIERFLNVFERVNAKQPFQTRFIIDHAETISERNIERIGAMGGGI
                     AIQHRMAFQGDYFIQRYGKEAAANTPPIKRMLELGVPVGAGTDATRVASYNPWVCLSW
                     LSTGKTLGGTALYEDSQLLDRETALALWSKGSAWFSGEQALKGALVEGEFADLAVLSD
                     DYFQVEAEDIQWIESVLTVVGGRVVYAGAEFKHDDPELPPASPDWSPVKRFGGAWRLS
                     EGKSAASNAPLQAASAACACSSQCGMHGHKHA"
BASE COUNT          642 a          683 c          581 g          653 t
ORIGIN      
        1 cgctgcgatt caaactacat cagggttttg agcagaggaa tcagcttttc gcccactacc
       61 atgcccaaca agcccaacaa agccacaaac ggcggcgcag gcgatttcac gttcaagagg
      121 taatacaaca cccccaccaa taccccgacc cccagcgaca atgcatacat tttcatcatc
      181 aatctttcat gtttagcggc ttaagctacc atcaaaatgc aaaacaagag cagcccaaag
      241 cgccccaaaa ggcatttttg ctnnnnnnnn nngcatgctt gtggccatgc atgccgcatt
      301 ggcttgagca agcacaggca gcagaagcgg cctgtaaagg cgcattcgat gccgcactct
      361 tgccctcaga caaacgccat gcaccgccaa agcgtttcac gggcgaccaa tcaggcgacg
      421 caggtggcaa ttcagggtca tcgtgtttga attcagcccc tgcatacacc acacgcccgc
      481 ccactaccgt aagcaccgac tctatccact gtatgtcttc ggcttcgact tggaaataat
      541 cgtctgacaa cactgccaaa tcggcaaact cgccttcaac caaagcccct ttaagcgctt
      601 gctcacccga aaaccaagcc gaacccttgc tccacaaagc caaggcagtt tcgcggtcta
      661 gtagttggct atcttcgtac aaagccgtgc cgcccaaggt tttgcctgtg cttaaccaag
      721 acaaacacac ccacggattg taagaagcca cacgcgtggc atctgtacct gcgcccacag
      781 gcacgcccaa ttccaacatg cgcttaatcg gcggggtatt ggcagcggct tctttgccat
      841 aacgctgaat aaagtaatca ccttgaaacg ccatgcggtg ttgtatcgcg ataccgccgc
      901 ccatcgcgcc gatgcgctca atattgcgct cagaaatggt ttctgcatgg tcgatgatga
      961 agcgggtttg aaacggttgt ttggcattga cccgttcaaa cacattcaaa aagcgctcga
     1021 tgctttcatc gtaagtggcg tgcaagcgaa acggccattt tttctccgca agcaattcga
     1081 cgatgcgctc caattcgtct tccattttcg gcaacaaatc aggacgaggc tctaagaagt
     1141 tttcaaaatc acctgccgac caagtgaggt tttcacccgc accattcatt tgcaaatagg
     1201 catcgccgtc gcctgggaag gtcatttgcg tccactcttg gtagctttgc aattcagagc
     1261 ctgctttttg agcaaacaaa ttgtaagcga tgcggatatt gagttgattg tcatcgtgta
     1321 agcgctgaat caccgcgtaa tcatcggggt agttttgccc gccaccgcct gcgtctatga
     1381 ccgaggtaat gcccaagcga ttgagttcgc gcataaaatg gcgggtggaa ttgacttggt
     1441 cttccaattc caatttcggc gccatgccca aggtggaata caaaatcatt gccgatggtg
     1501 tggcgatcag caagcctgtg ggctcgccgt gggcatcgcg ctgtatttgg ccgcctggtg
     1561 gattgggcgt atttttatca aagcccaaag ctttcaaagc cgcatgattg agcatggcgc
     1621 tgccgtacaa atgcaacaca aacacaggcg tatcaggcgc agctttgttg atttcatcca
     1681 aagtgggcaa gcgcttttcg gcaaattgaa attctgacca accgccgatg acgcgcaccc
     1741 attgcggcgc aggcgtgtta tcggcttgct ctttaagcaa gcgcatggca tcgctcaaag
     1801 aaggcacgcc ttcccaacgc aattccatat tgtaattcag gccgccacgt atcaaatgct
     1861 gatggctgtc attcaagcct ggtatcaccc gtctaccgtt caaatccacg attttggtat
     1921 cggcctgcgc cagcttcatc acatcttcat tgctgcctgt gcgcagcact ttgccatcgg
     1981 caatggcgat ggcttggact tcagtttgct tcttgtttaa ggtggtgatt ttaccgctct
     2041 tgagtatcag ttgcgcgtga gtcatgttta cctcgattgg cattatggaa aaagaaattg
     2101 gcagtgtggc gacttgtatt gacaaaacac aggatggagc ggtgttgcca gattaagttc
     2161 aagactttgt ttactgtcaa gtttcaaaca tagcggatat tgagctgcct cactcgcaaa
     2221 cacatcgaac caataacctc tataaaacgt ggtatttgct ctgctttatc aacatttctt
     2281 actctgtttt tttatcctta tccaatgcat aagccataca aaaaagcggc ctgtaacttt
     2341 acaggccgct atattcttca agccatgggc agggatacca gtattcttga ttgacacact
     2401 actcattcat tttgctagta tttgatgctt caggcttgtt aacgataggt ttttgaaaac
     2461 gcgatttaag gcattaaaag aatttttttg attgccaaat gttcacaggg catttgagtt
     2521 ttggaggcat cggcttttcc aacgccgtcc gctccaaggc ttcatctat
//
LOCUS       DAOMVK010000330         2566 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_11963_length_2566_cov_2.188371, whole genome shotgun sequence.
ACCESSION   DAOMVK010000330 DAOMVK010000000
VERSION     DAOMVK010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11963_length_2566_cov_2.188371"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PK856_09740"
     CDS             complement(<1..442)
                     /locus_tag="PK856_09740"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRL02689.1"
                     /translation="MKPNNLSFWMQSLDMSPYSASSDPALLLPSYQCDVAIIGGGFSG
                     LWLAYYLKLAQPETKIALFEAQHIGYGASGRNGGWLSSNIPSVINNLLKHPSITPAQI
                     QLLQRQVIDTIDVVDGVCQQENIDCDLHKGGLLFIATNEAQIERL"
     gene            complement(521..1666)
                     /locus_tag="PK856_09745"
     CDS             complement(521..1666)
                     /locus_tag="PK856_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HRL02690.1"
                     /translation="MLVAGGLAACGESSKPTSQTASAASTDNTPTTATAAKTAELNVY
                     NWSDYVDPNTVTEFEKNNGVKMRQDFYDSNEILEAKVLTGKSGYDLVFPSLSNLGRQI
                     KAGAYQKLDKSKIPNYQNIDPILLKLLSQVDPGNEYAVPYFWGFYTVGINPDKVKKAL
                     GTDTLPDNAWDLVFNPEYTKKLKSCGISYLDSATSVLPLALSYIGKDPNSNNPDDLKA
                     AAAMMEKTVPDVLRFSSSGYINDFAQGNLCVGIGYNGDFNIANTRAQAAKNGVKVVAL
                     VPKEGVGVWIDTVVITKDAKNVDNALKYINWTLEPKVAAQNGNLVNYAPGTNKAKEFM
                     KPEIVDNDSIFPSEEALKNSFVNTEKKPDTVKLGVRLWQQIKAKSHQ"
     gene            complement(1983..2333)
                     /locus_tag="PK856_09750"
     CDS             complement(1983..2333)
                     /locus_tag="PK856_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02691.1"
                     /translation="MSVSVYYTAKRTYPLTAAEIQAIHTLLEAFNADKGANSSEDMDF
                     YAAVGEYGSYEDGVLLQGALKPSRNLFKVRKNMRHILASLSAMKRAVPDSEWDVSLDD
                     LSAHWNEAKGFYFD"
     gene            complement(2364..>2566)
                     /locus_tag="PK856_09755"
     CDS             complement(2364..>2566)
                     /locus_tag="PK856_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02692.1"
                     /translation="PTSEGTYGQVIRMWHDDPQRPLEGKTFADWLETYIRGMEEGLYV
                     YSEDYGGIFKKEDIIADEDGEY"
BASE COUNT          598 a          635 c          614 g          719 t
ORIGIN      
        1 gcaagcgctc gatttgcgcc tcgttggtgg cgatgaacaa caagccgcct ttgtgcaaat
       61 cacaatcgat gttttcttgc tgacacacac catccaccac gtctatggtg tcgatgactt
      121 ggcgctgcaa caattgaatt tgcgcaggcg taatcgatgg gtgcttgagc aaattgttga
      181 tgacgctggg aatattggaa gagagccagc cgccattgcg ccccgatgca ccatagccta
      241 tgtgttgcgc ttcaaacagc gcgattttgg tttcaggctg cgcgagcttc aggtaataag
      301 ccagccacag gcctgaaaaa ccaccgccta tgatggccac atcgcattgg tagcttggta
      361 gcaataaggc ggggtcgctg gaggcggaat acggactcat gtccaaagac tgcatccaaa
      421 aagacaaatt attcggtttc atgcgcacac tcttgattca tgatggataa gcggcctgta
      481 aagagatcat aagtatttta caggccgcat tttggcaggt ttattgatgc gatttggctt
      541 tgatttgctg ccacaagcgc acaccgagtt tcaccgtatc gggctttttc tcggtgttca
      601 caaagctgtt tttcaaagct tcttcgctgg ggaagatgga atcgttgtcg acgatttctg
      661 gcttcatgaa ctctttggcc ttgttggtgc ctggtgcgta attcaccaaa ttgccgtttt
      721 gtgccgccac tttcggctcc agcgtccaat tgatgtattt gagcgcattg tcgacatttt
      781 ttgcatcttt ggtgatgacc acggtgtcta tccacacgcc cacgccttct ttgggcacca
      841 aggccaccac tttgacgcca tttttcgctg cctgcgcgcg agtattggcg atgttgaagt
      901 cgccgttata gcctatgccc acacacaaat tgccttgggc aaaatcgttg atgtagccag
      961 acgaactgaa gcgcaacacg tcgggcacgg ttttttccat catcgctgcc gccgctttca
     1021 aatcatcggg attgttggag ttcgggtctt tgccgatgta actcaaagcc aagggcaata
     1081 ccgacgtggc gctgtccaaa tacgagatgc cgcaagactt gagctttttg gtgtattcgg
     1141 gattgaacac caaatcccac gcattgtcgg gcaaggtatc ggtgcccaga gcttttttca
     1201 ctttgtcagg attgatgccc acggtgtaaa agccccaaaa atacggcacg gcgtattcat
     1261 tgccagggtc aacttgcgac agcaatttga gcaaaatcgg gtcaatgttt tggtaattgg
     1321 gtattttgga tttgtccaat ttttggtagg cacccgcctt gatttgtcgc cccaaattgc
     1381 tcaagctggg aaacaccaaa tcgtagcccg atttgcctgt taacaccttg gcttccaaga
     1441 tttcattgct gtcgtaaaaa tcttggcgca ttttcacacc attgtttttc tcaaactcgg
     1501 tgacggtatt ggggtcgaca taatccgacc aattgtaaac attcagttcg gctgttttgg
     1561 cggcggtcgc cgttgtagga gtgttgtctg tactggcggc ggaagcggtt tggcttgtcg
     1621 gtttactgga ttctccgcaa gcagccaaac caccagccac cagcattgcc aacaagatgt
     1681 gttgttcaag tttcatgatt tctccaatgt tcagacccaa ctttcgtttc agcttttata
     1741 gcccgcagca tcgctgggtt ttcaaacaaa aatggcttta aacaaccgtc tggtgcggtt
     1801 tgtgtttttt aatgtacaca gattaaactg aaaacaccag tttctcctca ccccaatact
     1861 gtaaaaactc ggcctaaata tcgttggctt gcgactgagt tactgcgaca aaaaagcggc
     1921 ctgtaaacgc tttgaggttt acaggccgct tgtttacttt aaatgcttaa gtttgcatgc
     1981 catcaatcaa agtaaaaacc cttggcttcg ttccaatggg cactcaaatc atccaggctc
     2041 acatcccatt cagaatcagg cacagcgcgt ttcatcgcac tcaggctagc caagatatgg
     2101 cgcatgtttt tgcgcacctt gaacaaattg cgactgggct tgagcgcacc ttgtagcaac
     2161 acgccgtctt catagctacc atactcaccc actgcggcat aaaaatccat gtcttcactg
     2221 ctgttggcgc ctttgtcagc attgaatgcc tcaagcaagg tgtgaatggc ttggatttca
     2281 gcggcagtta atggataagt gcgtttggct gtgtaataca ccgagaccga catgtgcgct
     2341 cctagggctg cccattgatg ggcttaatat tcaccgtctt catcagcgat gatgtcttct
     2401 tttttgaaaa tgccaccata atcttctgaa tacacataca agccttcttc catgccgcgt
     2461 atgtaggttt ctagccagtc ggcaaaagtc ttgccttcca aaggacgctg cgggtcgtcg
     2521 tgccacatgc ggatgacttg gccatacgtg ccttcagaag tgggat
//
LOCUS       DAOMVK010000331         2549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_12074_length_2549_cov_2.919407, whole genome shotgun sequence.
ACCESSION   DAOMVK010000331 DAOMVK010000000
VERSION     DAOMVK010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12074_length_2549_cov_2.919407"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PK856_09760"
     CDS             <1..403
                     /locus_tag="PK856_09760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003743301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRL02693.1"
                     /translation="GFDAAQYYHPQRHMGTLIGHYRHHTVHDPFFHIGLTDLTAHVNF
                     TDIAQAGTNAGLDLIGYATQAHFLLNLGITDIMAHHADPASADYIRSAAAIHKLTGQH
                     EMGELFKVIAFGRNIGIDWQGFSHGDICHKL"
     gene            531..607
                     /locus_tag="PK856_09765"
     tRNA            531..607
                     /locus_tag="PK856_09765"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:565..567,aa:Met,seq:cat)
     gene            838..1215
                     /locus_tag="PK856_09770"
     CDS             838..1215
                     /locus_tag="PK856_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRL02694.1"
                     /translation="MNQVCYFEIQSSQPTREVAFYQAVFGWDFEKAVGLPIDYYRITT
                     EGIHGGLLSRPAATPPTHCGTNAFTCSIEVEDFDATAALILAHGGQVAMPKFAIPNQC
                     WQGYFVDADHNTFGIVEPNPNAA"
     gene            complement(1305..>2549)
                     /gene="prpD"
                     /locus_tag="PK856_09775"
     CDS             complement(1305..>2549)
                     /gene="prpD"
                     /locus_tag="PK856_09775"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HRL02695.1"
                     /translation="SYCLDPVQAAWNIGCIIRWLDYNDTWLAAEWGHPSDNLGAILAV
                     ADYLSQQRIAQGEAPLTMKDVLIGMVQAHEIQGVLALENSFNRVGLDHVMLVKVASTA
                     VAAKMLGATREQLLSAVSHAFVDGQALRTYRHAPNAGSRKSWAAGDATSRGVFLAFVA
                     LRGEMGIPGVLTAPQWGFYDILFSHTNKDLALKPEGEREFSLPQPFGDYVMENVLFKI
                     SFPAEFHSQTACEAAVRLHPQVKNRIDDIEKIVITTHESAIRIISKVGELANPADRDH
                     CLQYMTAVPLLFGDLVAEHYEDSFHAAHPEIDVLRDKMEIAEDLRYSREYLESDKRSI
                     ANALQVFFKDGSSTELIEIEYPVGHRRRRQEGIPLLEAKFKANLATRYVPQRSAAIFD
                     LCADADKLNATAVHRFVEAFVL"
BASE COUNT          651 a          731 c          553 g          614 t
ORIGIN      
        1 cggcttcgat gccgcccagt attaccatcc gcagcgccac atgggcacgc tgatcggcca
       61 ttaccgccac cacaccgtgc acgacccgtt tttccacatc ggcctcaccg acctcaccgc
      121 ccatgtaaac ttcaccgaca tcgctcaagc cggcaccaat gccgggctgg atttaatcgg
      181 ctatgccacg caagcccatt ttctgctcaa cctcggcatc accgacatca tggcccacca
      241 cgccgacccg gcttctgccg actatatccg cagtgccgcc gccatccaca aactcaccgg
      301 ccaacacgaa atgggcgaat tattcaaagt gattgctttc ggccgcaata tcggcatcga
      361 ctggcagggc ttcagccacg gcgacatttg ccacaaattg tgataaaaag gccgtctgaa
      421 aaggtttcag acggcctttt tatttaaata aatcaaacaa caaaaaaatt atcatcaaat
      481 tatatttgac agccaaacac cattccctta tagtacgcgc ttcaccagtt ggcgaattag
      541 ctcagtcggt tagagcagag gaatcataat ccttgtgtcc ggggttcgaa tccctgattc
      601 gccaccaaat attaaaaacc acatcttcgg atgtggtttt ttcatttccg agtttccact
      661 tttcaccaca ctacccacca aaaccctgtt ttcaaaaaat atcacacgcc cttcgccaca
      721 ccatcatcac aaccaggcat ttatattgcc cttgccttct tgtttatcga actctcacca
      781 accaactttc tccaacctct ctaaacctcc taactcacca cctataaggg gatgcatatg
      841 aaccaagtgt gttattttga aattcaatca tcacaaccca cacgcgaagt ggcattctat
      901 caagccgtgt ttggttggga ttttgaaaaa gcagtaggct tgcccataga ctattaccgc
      961 atcaccactg agggcataca cggcggcttg ttatcacgcc ctgcagccac accaccaact
     1021 cattgcggca ccaatgcttt tacttgctca attgaagtcg aagattttga tgccactgcc
     1081 gcattaatcc ttgcccatgg cggccaagtg gccatgccca aattcgccat tcccaatcag
     1141 tgttggcaag gctattttgt ggatgcagac cacaatactt ttggcattgt tgaacccaat
     1201 ccaaatgccg cttaaagcta tacatgaata agcggcctgt aatgtttaca ggccgcttat
     1261 ttaaatggca aatagataaa gacatcctct atataccaac cttcttacaa cacaaaggcc
     1321 tctacaaaac ggtgtaccgc cgtggcattg agcttgtctg catccgcaca caaatcaaaa
     1381 atcgcggcgc tgcgttgcgg cacatagcgg gtggccaaat tggctttgaa tttcgcttcc
     1441 aacagcggta tgccttcttg gcgacgacga cggtggccta ctggatattc gatttcaatc
     1501 aactctgtgg atgagccatc tttgaagaac acttgtaagg cattggcaat ggagcgcttg
     1561 tcgctttcca aatactcacg gctgtaacgc aggtcttcgg caatttccat tttgtcgcgc
     1621 aacacatcga tttcagggtg ggccgcgtga aagctgtctt cataatgttc ggccaccaag
     1681 tcgccaaaca acaaaggcac ggccgtcatg tattgtaagc aatggtcgcg gtcagcaggg
     1741 ttggccaatt cgcccacttt ggaaatgatg cgtatcgccg actcgtgcgt ggtgatgacg
     1801 attttctcta tgtcgtctat gcggtttttc acttgcgggt gcaagcgcac agcggcctca
     1861 caagcggttt gggaatggaa ttcggcaggg aaagaaattt tgaacaacac gttttccatc
     1921 acatagtcac caaacggctg tggcaatgaa aattcgcgct caccttcagg cttcaaagcc
     1981 aaatctttgt tggtgtggct gaacaaaatg tcgtaaaaac cccattgcgg tgcggtcaac
     2041 acgccaggga tgcccatctc gccgcgcaag gccacaaatg ccaagaacac gccgcgactg
     2101 gtggcatcgc ctgccgccca tgatttgcgt gagcctgcat ttggggcatg gcggtaagtg
     2161 cgcaaggctt ggccatcgac aaacgcatgc gacacagctg ataagagttg ctcacgtgtg
     2221 gcgcccaaca ttttcgccgc caccgcagta gaagccactt tcaccaacat cacgtggtct
     2281 aatcccacgc ggttaaacga attttccaaa gccaacacac cttgaatttc gtgcgcttgc
     2341 accatgccga tgagcacgtc tttcatcgtc aacggggctt cgccttgggc gatgcgctgt
     2401 tgcgacaaat aatccgccac agccaaaatc gcgcccaaat tgtctgatgg atggccccac
     2461 tctgctgcca gccatgtgtc gttgtaatcc aaccaacgga tgatgcagcc tatgttccaa
     2521 gcggcctgta cgggatctag gcaatacga
//
LOCUS       DAOMVK010000332         2543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_12119_length_2543_cov_3.914389, whole genome shotgun sequence.
ACCESSION   DAOMVK010000332 DAOMVK010000000
VERSION     DAOMVK010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12119_length_2543_cov_3.914389"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1345
                     /gene="glmU"
                     /locus_tag="PK856_09780"
     CDS             2..1345
                     /gene="glmU"
                     /locus_tag="PK856_09780"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HRL02696.1"
                     /translation="MILAAGKGTRMYSKLPKVLHQIGGLPLVQHVINTAQSLEPASIQ
                     VVIGHGKEQVQAQIQSDVSWVVQDQQLGTGHAVKCALPNLPIDGKTLILYGDVPLTDT
                     ETLQQLLQLADDEVGLLTDALDNPQGLGRIIRNEQGICAIVEEKDATAEQKAITEINT
                     GILVLPNSHLEKWLSTLQNSNAQGEYYLTDVIALAVADGITVHGMTVRASHLAAGINN
                     KLQLAEIERIYQLNQARALMTAGVTLRDPNRFDLRGSLSHGQDCIFDVGVVVEGTCVL
                     GDDVNIGAHCVLSNVTIGSGCHIAPFSHLSDCVVGSDARIGPFARLRPNAHLGDGVHI
                     GNFVEVKNTTIGHGSKVNHLTYLGDAAVGNNSNIGAGSITCNYDGVNKFKTVIGNDVR
                     IGSGTMMVAPLSIGDKATTGAGSVITKDCPAGQLTIGRTRQVSIEKWQRPEKDSK"
     gene            1390..>2543
                     /gene="glmS"
                     /locus_tag="PK856_09785"
     CDS             1390..>2543
                     /gene="glmS"
                     /locus_tag="PK856_09785"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRL02697.1"
                     /translation="MCGIVGAIRQTSNAVEFLVEGLKRLEYRGYDSSGIAVNTADGIE
                     RVRRVGRVASMEEAANEVGLSGYLGIGHTRWATHGGVTEPNAHPHVSNDLIAVVHNGI
                     IENFESERVRLQGLGYVFSSQTDTEVIAHAVHHEYTHAHQGDLFAAVQAACRHFHGAY
                     AIGVIAQDQKDELIVARMGCPLLVALGENETFIASDVSAVIAQTRRVVFLEDGDVARL
                     TTAGIAHLVDKTGNTVERAVRVSELSLASLELGPYNHFMQKEIHEQPKAIADTAEVLI
                     DDAFHPEIFGEKATEVFNDIHSIKILACGTSYYAGLTAKYWLESIAKIPCDVEIASEY
                     RYRDVIADPKQLIVTISQSGETLDTMEALKYAQSLGHTHSLSICNVMESA"
BASE COUNT          661 a          629 c          652 g          601 t
ORIGIN      
        1 tgtgatttta gcggccggta aaggcacgcg catgtattcc aaattaccca aagtgttgca
       61 ccaaattggc ggtttgcctt tggtgcaaca cgtgattaac actgctcaaa gtttggagcc
      121 tgcttcgata caagtggtga taggccacgg caaagagcaa gtgcaagcgc agattcaaag
      181 cgatgtatct tgggtggtac aagaccaaca attgggcact ggccacgcag tgaaatgtgc
      241 cctacccaat ttgcccatag atggcaaaac cctgattttg tacggtgatg tgccattgac
      301 cgacaccgaa accttacagc aattgctgca attggctgat gatgaagtgg gtttactcac
      361 cgatgcgttg gataacccac aaggcttagg tcgcatcatt cgcaatgagc aaggcatttg
      421 cgccattgtg gaagaaaaag atgccacagc tgagcaaaaa gccatcaccg aaatcaacac
      481 tggcattttg gttttgccca acagccattt ggaaaaatgg ttgtctacct tgcaaaacag
      541 caatgcccaa ggcgagtatt acttgaccga tgtgattgcc ttggctgtag ccgatggcat
      601 tactgtgcat ggcatgacgg tgcgtgcaag ccatttggct gctggcatca acaacaaatt
      661 gcaattggcc gaaattgaac gcatctacca attgaatcaa gcccgtgctt tgatgaccgc
      721 aggggtcact ttacgcgatc cgaatcgttt tgatttgcgt ggcagcctca gccacgggca
      781 agattgcatt tttgatgtgg gcgtggtggt agaaggcacg tgtgtcttgg gcgatgatgt
      841 gaacattggc gcacactgtg tgctcagcaa tgtcaccata ggctcaggct gccacattgc
      901 cccgttcagt cacttaagtg attgtgttgt gggctcagat gcgcgcattg gcccatttgc
      961 ccgcttgcgc cccaatgccc atttgggtga tggtgtgcac atcggcaatt ttgttgaagt
     1021 gaaaaacacc accattggtc atggttctaa agtcaaccac ttaacctatt tgggcgatgc
     1081 tgccgtgggc aacaattcca atataggtgc aggttcgatt acctgcaatt acgatggggt
     1141 gaacaaattt aaaaccgtca tcggcaatga tgtgcgcatc ggctcaggca cgatgatggt
     1201 agcaccattg agcataggcg acaaagccac cacaggtgct ggcagtgtga ttaccaaaga
     1261 ttgtccagca ggacagttga ccataggtcg cacgcgacaa gtcagcattg aaaaatggca
     1321 acgtcccgaa aaagacagca aataatcaca ccaaattcat ttttaagatt tctaagataa
     1381 ggaagcagta tgtgcggcat tgttggcgcc attcgccaga cttcaaacgc agttgaattt
     1441 ttagtagaag gtttaaaacg tctcgaatac cgtggctacg actcatcagg catcgcggtc
     1501 aacaccgctg atggtattga acgcgtgcgc cgtgtcggcc gcgtggcctc gatggaagaa
     1561 gccgccaacg aagtgggctt gagcggttat ttgggcatag gtcacacccg ttgggccacc
     1621 catggcggcg tcaccgaacc caatgcgcat ccgcatgtat ccaatgattt gattgcagtg
     1681 gtacacaacg gcatcattga aaactttgaa agcgaacgcg tgcgcttaca aggtttgggc
     1741 tatgtgttca gctcacaaac cgacaccgaa gtgattgccc acgctgtgca ccacgaatac
     1801 acccacgctc atcaaggcga tttgtttgca gcggtacaag cggcctgtag acacttccac
     1861 ggcgcttacg ccataggcgt gattgcacaa gaccaaaaag acgaattgat tgtggcccgc
     1921 atgggctgcc cactgttggt ggccttgggt gagaacgaaa ccttcatcgc ctctgatgta
     1981 tccgcagtaa tcgcccaaac ccgtcgcgtg gtgtttttgg aagacggtga tgtggcccgt
     2041 ttgaccaccg ctggcatcgc tcacttggtg gacaaaacag gcaacaccgt cgaacgtgcc
     2101 gtgcgcgtgt ctgagttgtc attggcctct ttggaattgg gcccttacaa ccatttcatg
     2161 caaaaagaaa tccacgagca accgaaagcc attgccgaca ctgccgaagt gttgattgat
     2221 gacgcttttc acccagaaat ctttggtgaa aaagccacgg aagtgtttaa cgacatccac
     2281 agcatcaaaa tcttggcctg tggcacctcg tattatgcgg gtctgactgc caaatactgg
     2341 ttggaaagca tcgccaaaat cccttgcgat gtcgaaatcg ccagcgaata ccgctaccgc
     2401 gatgtgattg ccgatccgaa acaattgata gtcaccatct cccagtctgg cgaaacccta
     2461 gacaccatgg aagccttgaa atacgcacag tctttgggcc atacccacag cttgtcgatt
     2521 tgcaatgtga tggaaagcgc ctt
//
LOCUS       DAOMVK010000333         2543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_12122_length_2543_cov_2.271302, whole genome shotgun sequence.
ACCESSION   DAOMVK010000333 DAOMVK010000000
VERSION     DAOMVK010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12122_length_2543_cov_2.271302"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="PK856_09790"
     CDS             <1..897
                     /locus_tag="PK856_09790"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRL02698.1"
                     /translation="EWRWRGTMAVLLFATYALMFSLMWTLVHTLLRWMVARRLLNRSV
                     LVPVAWLAAIAAGLYNAYVPTVVHYQVKIDKPLAQPLRIALVSDTHLGRFIGARHLRE
                     LQTILKRERADVLLLAGDVMDDVPTVYRKQHMARLMSGLKTPLGQYAVLGNHDNYGGE
                     QQGIVKDLQAAGFTTLRDEHTTVNQQFVLVGRRDKVENRASAAQVIPKSDLPVIVMDH
                     QPADMDAIANTRADLVVSGHTHRGQVFPATLLIKYFQTYPYGHYRIDNSQLVVTSGYG
                     LWGLPFRLGARSEVAIIELIGK"
     gene            992..1462
                     /gene="ribH"
                     /locus_tag="PK856_09795"
     CDS             992..1462
                     /gene="ribH"
                     /locus_tag="PK856_09795"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRL02699.1"
                     /translation="MQTLAVNHQGSSLRIGIVQARFSDGIGSHLRESCIRKLLALGVQ
                     EDNITVATVPGALEVPLVLQNLAATEQYDALIALGIIIRGETYHFELVSNESGAGVTR
                     VGLDFNIPIANAILTTENDDQAIARMNIKGEDAAVVAVEMANLLTTIHNTDAAE"
     gene            1473..1901
                     /gene="nusB"
                     /locus_tag="PK856_09800"
     CDS             1473..1901
                     /gene="nusB"
                     /locus_tag="PK856_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRL02700.1"
                     /translation="MKTARRRAREFVVQGLYQAQLNSALTASNIRSNLLDNEQFLKAD
                     EPLFAQVFDGAFETQTELLQLITPHLDRDLAEVSPIERAVLLMATFELREMPETPYPV
                     IINEAIEITKTFGGTDGFKFVNGILDKIAPQLRPNDPPRK"
     assembly_gap    2013..2022
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2086..>2543)
                     /locus_tag="PK856_09805"
     CDS             complement(2086..>2543)
                     /locus_tag="PK856_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HRL02701.1"
                     /translation="LLDPADSITEFEQAMGMSLPTSLREFLRLYQVDDFAMANVQFGQ
                     GEAYLHQMFAMNHQDALCPWWQGKMRPVNQLLMALTDGYALILDVNTGAVWALDQDNS
                     GMDLVAHDFEDFFRALATVYLSEVGLDEVLNQLNVDSSGFWLQQDRLKA"
BASE COUNT          655 a          666 c          606 g          606 t
ORIGIN      
        1 gaatggcgtt ggcgcggcac catggcggtg ttgttgttcg ccacttatgc gctgatgttc
       61 tcgctgatgt ggacgcttgt gcacacgctc ttgcgctgga tggtggcgcg cagactgctc
      121 aatcgcagcg tactcgtgcc tgttgcttgg ctggcggcca tcgccgcagg gctttataac
      181 gcgtatgtgc ccaccgtggt gcattatcaa gtcaaaatcg acaagccctt ggcccaaccc
      241 ttgcgcatcg ccttggtcag cgacacccat ttgggccgct tcataggcgc gcgccatttg
      301 cgtgaattgc aaacgatatt aaaacgcgaa cgtgccgatg tgcttttgct cgcaggcgat
      361 gtgatggacg acgtgcccac ggtataccgc aagcaacaca tggcgcgctt gatgagcggc
      421 ctgaaaacgc cattgggcca atatgcggta ttgggcaatc acgacaatta cggtggtgag
      481 caacaaggca ttgtcaaaga cttacaggcc gctggtttca ccaccctcag agacgaacac
      541 acaacggtga atcagcaatt tgtgctggtg ggcaggcgcg acaaggtgga aaaccgtgcc
      601 agtgccgccc aagtcatccc caaatccgat ttgcccgtga ttgtgatgga ccatcagcca
      661 gccgatatgg acgcgatagc caatacccgc gccgatttgg tggtgtcagg tcatacccac
      721 cgtggccaag tgtttcccgc tacgttgctc atcaagtatt tccaaaccta tccttacggg
      781 cattaccgca tagacaacag ccaattggtg gtgacctcgg gatatggctt gtggggcttg
      841 ccatttcgct tgggcgcgcg ctcagaagtg gccatcatcg aattaattgg caaatagcta
      901 caggattggt gccctgttac gctataatac ggcattcgtt tatcgacggc gcttctcaca
      961 agcgtcgttg tctttcataa aggatccata catgcaaacc ttagcggtta atcatcaagg
     1021 cagctcattg cgcatcggca tcgtacaagc ccgtttttca gacggcatcg gcagccattt
     1081 gcgcgagtct tgcatccgca aattactggc tttgggtgta caagaagaca acatcaccgt
     1141 ggccaccgtg ccaggtgctt tggaagtacc gttggtattg caaaatttag cggccacaga
     1201 acaatacgat gccttgatag ccttgggcat catcatccgt ggcgaaactt accattttga
     1261 actggtttcc aacgaatcag gtgcaggcgt gacccgtgtg ggtttggatt tcaacatccc
     1321 catcgccaat gccatcttga ccactgaaaa cgatgaccaa gccattgcac gcatgaacat
     1381 caaaggtgaa gatgccgcag tggtggcagt ggaaatggcc aatttattga ccaccatcca
     1441 caacacagac gcagcagaat aaggaattaa gcatgaaaac agcacgccgc cgcgcgcgtg
     1501 aatttgtggt acaaggtttg taccaagcac aattgaacag tgcactcaca gccagcaaca
     1561 ttcgcagcaa cttgctcgac aacgaacaat tcttaaaagc cgatgagcca ttgtttgctc
     1621 aggtttttga tggcgcgttt gaaacccaaa ccgaattgtt gcaattgatt accccacatt
     1681 tagaccgtga tttggctgaa gtcagcccga ttgaacgcgc ggtattgttg atggccacgt
     1741 ttgaattgcg tgaaatgcca gaaacccctt acccagtcat catcaacgaa gccatcgaaa
     1801 tcaccaaaac ctttggtggc accgatggtt tcaaatttgt gaacggtatt ttggacaaaa
     1861 ttgccccgca attgcgccct aacgacccac cacgcaaata aacttgatac tgtttgagtg
     1921 aaataaataa gcggcctgta aatcattaca ggccgctttc aatgaatcat ctcacttatc
     1981 atggtttaac agtaacagtg gcttggtgta aannnnnnnn nnaagcgtca tcacaagcgg
     2041 cctgtcaatg tattaaagcg tttacaggcc gctttgtgtg cacattcaag cttttaatcg
     2101 atcttgctgc aaccaaaagc cacttgaatc cacatttaat tggttcaaca cctcatccaa
     2161 acccacctca ctcaaataca ccgtagccag cgcgcggaaa aaatcctcaa aatcatgcgc
     2221 caccaaatcc atgccactgt tgtcttggtc taaagcccag acagcgcctg tgttgacatc
     2281 caaaatcaat gcatagccgt cggtcaaagc catcaacaat tggtttacag gccgcatctt
     2341 tccttgccac cacgggcata aggcatcttg gtgattcatg gcaaacattt ggtgtaaata
     2401 agcctcgccc tgaccaaact gcacattggc catggcaaaa tcatccactt ggtagagcct
     2461 taaaaattcc cgcaatgaag tgggtaaact catgcccatg gcttgctcaa attcggtgat
     2521 gctgtcggct gggtcgagta aat
//
LOCUS       DAOMVK010000334         2532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_12193_length_2532_cov_3.523214, whole genome shotgun sequence.
ACCESSION   DAOMVK010000334 DAOMVK010000000
VERSION     DAOMVK010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12193_length_2532_cov_3.523214"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(203..1189)
                     /locus_tag="PK856_09810"
     CDS             complement(203..1189)
                     /locus_tag="PK856_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02702.1"
                     /translation="MRKTFNIKTLPNWVFAVLIAAFCWDDAQDEALFAHFGVWLALLT
                     ALNVYVAVQKNTQGEWEWTQEERYRCGFAVVSAAIIAGLRQGFGAEDASVLFWLLVYA
                     GVNWAWAYGVKKYLAQQSDWQEHYLSAYQQHDFAPLRIVIHSHSERQQKWQVVGVCVA
                     MMVFGLWLVDVKNSEYVSVADKWVDAITTLFMLGFVLRQCKERNNLLKALRLLEDTEG
                     EYLDISSQGLSWQVLVNQSQGKWQQLMHPQLVLDAQAIAWPQLKSVTVLADDDNAAHP
                     ITYLLIQAKREHAIKPLTLRIDEKQTNHTSKQLRQIVLQYHNLGMKPFHTVA"
     gene            complement(1512..2018)
                     /locus_tag="PK856_09815"
     CDS             complement(1512..2018)
                     /locus_tag="PK856_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02703.1"
                     /translation="MYIKWFIGMVLLWVSFSSFAHKDARLKVDEHGIVSRIPAEFGEV
                     KLNIQGLGTKKKALTFIVNEQHTQVPECVANLIRTDSPQGMQLSGSWYHETSILPYYV
                     SVQFYTPAQVQAQNYNNPDLSLLFDLQNSQLLMVDSFKLAETSVESICKKIQINRERQ
                     AHKQTILP"
     gene            complement(2113..>2532)
                     /locus_tag="PK856_09820"
     CDS             complement(2113..>2532)
                     /locus_tag="PK856_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HRL02704.1"
                     /translation="LQKESFHTSLLNIALDGKETQVIVRDFQMHPFKPAVLHVDFQAV
                     DAAKEIKIKVPVHFINAESSPAIKLQGGRVSQLIQSIEVLVTAGNVPAELVVDLKTAT
                     AGQIVHISDIAFPEGVQSVSLKRDENLAIAAITGKKR"
BASE COUNT          677 a          647 c          531 g          677 t
ORIGIN      
        1 gctcacatac cacaccagcg cactgccaag aaacaatatc catactggaa tattcagatt
       61 caccgcagtc ggcacacgca tacttatttt cccctgctct gtgtcttgtc atcgcctgat
      121 tgccgtaagc atcgccatca gaccttgccg tcatacataa caagcagcct gtaaacctta
      181 caggccgcct ttaaaagaat tttcaagcca cggtatgaaa cggcttcatg cccagattgt
      241 ggtattgcaa tacaatttga cgcaattgct tggaggtgtg gttggtctgt ttttcatcaa
      301 tgcgcaaggt caaaggcttg atggcatgct cgcgcttggc ctgtatcagc agatatgtga
      361 tgggatgagc ggcattgtca tcatctgcca aaaccgtcac actcttgagc tgtggccaag
      421 caatggcctg cgcgtccaat accaactgag ggtgcatcag ctgctgccac ttcccttggc
      481 tttggttcac caaaacttgc caagacaagc cttgcgagct gatgtccaaa tactcgcctt
      541 ccgtatcttc cagtaaacgc aaggctttga gcagattgtt gcgctctttg cattggcgca
      601 gcacaaaacc caacatgaac aatgtggtga tggcgtccac ccatttgtct gccacgctta
      661 catactcgct gtttttcaca tcgaccaacc acagcccaaa taccatcatg gccacacaca
      721 cgcccactac ctgccatttt tgctgtcgct cactgtggct gtgtatgact atgcgcagcg
      781 gtgcaaaatc gtgttgctgg tatgcggaca aataatgctc ttgccaatcg ctttgctgcg
      841 cgaggtattt tttcacccca taagcccaag cccagttcac accagcgtaa accaagagcc
      901 aaaacaacac agatgcgtct tcggcaccga aaccctgtct taagccagca ataatggccg
      961 cactcaccac cgcaaatccg caacggtagc gctcctcttg cgtccattcc cactcgcctt
     1021 gggtattttt ttgtaccgcg acgtacacat tcaaggcggt taacaaagcc aaccacaccc
     1081 caaaatgggc aaataaggcc tcatcctgcg catcatccca acaaaaagcc gcaatcaaca
     1141 cggcaaaaac ccaattgggc agggttttga tgttgaaggt tttcctcacg aagtcctcca
     1201 ttgtttgatt tgtctgtcag gccaagacta gcacagagcc aatctagctt gcagccatgt
     1261 tcataagcgg cctgtaagcg gttttaaggt aggtctggat gaaacaaagg cttagccaca
     1321 agtatctgtt tagatgaagg ggcagataca aatggttacg catgaaaaag cggtctgtaa
     1381 acatttacag gccgctttga aatcattgtc acggcatctg cccgcagagc cgctcattta
     1441 agtaacaaca ccttaactct cacaaactca ctgtatcgtc atacaacaaa tacactgtgc
     1501 ccctaatcgc atcaaggcag tattgtttgt ttatgtgctt gccgctcacg gttgatttgg
     1561 attttcttac aaatgctttc aaccgaagtt tcagccaact tgaaactatc gaccatcaaa
     1621 agctggctat tttgcaaatc gaacaacaag gacagatctg gattattata attttgagcc
     1681 tgcacctgtg ctggggtata aaattgcaca gaaacgtaat agggcaatat actggtttca
     1741 tgataccaag agccactgag ttgcatacct tgtgggctgt cagtacggat taaattggcc
     1801 acacattcag gcacttgagt gtgctgctcg tttacaatga aagtcagcgc ctttttcttt
     1861 gtccccaagc cttggatgtt gagtttcact tctccaaact cagcaggaat gcgtgaaact
     1921 ataccgtgtt cgtctacttt cagcctggca tctttatggg caaaactgct aaaactgacc
     1981 cacagtaaaa ccatgccaat gaaccatttt atatacatac catttcccct atgttctcac
     2041 gccgaaactg cacataaaaa agcggcctgt aaacatttac aggccgcttt taaactgttt
     2101 taaacgttaa gattaacgtt ttttaccagt gatggctgca atcgccaagt tttcgtcacg
     2161 tttcaatgac acgctttgta cgccttctgg gaacgcgatg tcagagatgt gaacgatttg
     2221 acctgcagtg gcagttttca aatccactac caattcagca ggcacgttgc cagcagttac
     2281 caatacttca attgattgga tcaattgaga tacgcgaccg ccttgcaatt tgattgctgg
     2341 agatgattca gcattgatga agtgtactgg cactttgatt ttgatttctt tggcagcgtc
     2401 aacggcttgg aaatccacgt gcaacacagc tggtttgaac gggtgcattt ggaaatcacg
     2461 tacgatgact tgggtttctt taccgtccaa agcgatgttc aacaatgaag tgtggaaaga
     2521 ttctttttgc aa
//
LOCUS       DAOMVK010000335         2531 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_12198_length_2531_cov_2.970517, whole genome shotgun sequence.
ACCESSION   DAOMVK010000335 DAOMVK010000000
VERSION     DAOMVK010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12198_length_2531_cov_2.970517"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1525
                     /locus_tag="PK856_09825"
     CDS             <1..1525
                     /locus_tag="PK856_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HRL02705.1"
                     /translation="RALEASRRNRRDVVGKVLTTFSQCLVAFNIEAQIKGREKNLFSI
                     HSKMMQKNLRFAEVMDIYGFRVIVNNVPTCYTALGALHSIYKPKPGKIKDYIAIPKNN
                     GYQSIHTTLVGPYGLPIEVQIRTREMDEIAEGGVASHWMYKSNGASFDAASVRTHQWM
                     QNILDLQAESDNAIEFLEHVKVDLFPDEVYLFTPKGKIIVLPRGATPVDFAYAVHTDI
                     GNHCVAARVNNNMVPLRTVLNTGDTIEVITSTKSNPNPAWLNFVVSGRARSGIRAWIK
                     TMNRHDAVILGESLLNKALVSLLPKQLLLSEELRDQYLNQLAHKNTNFEDVLYEVGMG
                     RLLPVVVAKDLAKLAGETSYEIGNINPVKIYGTQNGNINLAQCCRPIPGDAIRAQLAE
                     NQGILVHSDQCATILSIDADHQLPANWDLIDTEQLYPVHLLINARDKQGLLAALASAM
                     SGAGANILSVETPSTTVDGIEGFIEFRFKLEVKNLQHLQKVSKAIEQIPQVRKVNRA"
     gene            1761..1973
                     /gene="rpsU"
                     /locus_tag="PK856_09830"
     CDS             1761..1973
                     /gene="rpsU"
                     /locus_tag="PK856_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRL02706.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRGVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKKLRSQTLPPKLF"
     gene            2042..2488
                     /locus_tag="PK856_09835"
     CDS             2042..2488
                     /locus_tag="PK856_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HRL02707.1"
                     /translation="MQTKDRINQDMKDAMRAKETEKLSTIRMLMAAIKQVEVDERVEV
                     DDTRIIDIVTKMIKQRHDSVKIYRDNNHNEAADKEQAEIDLLHNYLPAQLSEAEVKAE
                     IAAAVAATGASSMAQMGQVMAILKPKLNGVADMGQVSAWVKAALNS"
BASE COUNT          752 a          619 c          552 g          608 t
ORIGIN      
        1 ccgtgcctta gaagcctcac gccgcaaccg ccgtgatgtg gtgggcaagg ttttgaccac
       61 tttcagccag tgtttggtgg ccttcaatat tgaggcacaa atcaaagggc gtgaaaaaaa
      121 cctgttcagc atacacagca aaatgatgca aaaaaatttg cgtttcgctg aagtgatgga
      181 tatttacggt tttcgcgtga ttgtgaacaa cgtacccact tgctataccg cactgggcgc
      241 tttacacagc atttacaagc ccaagcctgg aaaaatcaaa gactacatcg ccattcccaa
      301 aaacaatggt tatcaaagca tacacaccac tttggtaggc ccgtacggct tgcccataga
      361 ggtgcaaatt cgcacccgag aaatggacga aattgccgaa ggcggcgtgg ccagccattg
      421 gatgtacaag tccaatggtg cctcatttga cgcagcctct gtgcgcaccc atcaatggat
      481 gcaaaacatc ttggacttac aggccgagag cgacaacgcg attgagtttt tagaacacgt
      541 taaagtcgat ttattccctg acgaagtgta tttgttcaca cccaaaggca aaatcatcgt
      601 gttaccacgc ggcgctaccc ctgttgactt cgcttacgcc gtacacacag acataggcaa
      661 ccattgcgtt gctgcgcgcg tgaacaataa tatggtgcca ctgcgtactg tgttgaacac
      721 gggtgacacc attgaggtca tcacctcgac caaatccaat cctaaccctg catggttgaa
      781 ttttgtggtc agcggtcgcg cccgcagtgg catacgcgca tggatcaaaa ccatgaaccg
      841 ccatgacgca gtgattttgg gcgaaagcct gcttaacaaa gcattggtga gcttattgcc
      901 caaacaattg ttattgtccg aagaattgcg tgaccaatac ttaaaccaat tggcgcacaa
      961 aaacaccaat tttgaagatg tgttgtacga agtgggtatg ggccgcttat tgcccgtggt
     1021 cgtggccaag gatttggcca aattggcagg tgaaacctct tacgaaatcg gcaacatcaa
     1081 tcctgttaaa atctacggta cccaaaatgg caacatcaat ttggctcagt gttgtcgccc
     1141 tattcctggc gatgccatcc gtgctcaatt ggcagaaaac caaggcatct tagtacacag
     1201 cgaccaatgc gccaccattt tgtccatcga tgcagaccac caactgcctg ccaattggga
     1261 tttaattgat actgaacaac tctatcccgt acacctgctc atcaatgctc gtgacaaaca
     1321 aggtttattg gctgcactcg ccagcgccat gtcaggtgct ggtgcaaaca tcttgtcagt
     1381 cgaaacccct tccaccacgg tggatggcat cgaaggcttt attgagttcc gcttcaagct
     1441 agaagtaaaa aacctccaac acttgcaaaa agtctccaaa gccatagagc aaattccgca
     1501 ggtacgcaaa gtcaaccgtg cttaaattat tctagattta caggccactt gaaccaatcc
     1561 acatcggcct gtaaacagta ttgtcttgac aagcaagctt aaactcatta tcatagtcaa
     1621 ttcttttaat gttttagctt gtctgggggt gattttttat tggcgtagcc ttacgaggcc
     1681 ggcctaaaac tttggcacat tgccaaaatc actttgtagt atccatattt taataaaatt
     1741 gattgataag gatttcccga atgccagcta ttcgtgtaaa agaaaatgaa ccatttgaag
     1801 ttgcgatgcg tcgctttaaa cgcggtgttg aaaaaacagg cttgttaact gagttacgtg
     1861 ctcgtgaagc ttatgaaaaa ccaacaaccg aacgcaaacg caaaaaagca gctgctgtta
     1921 aacgcttgca aaagaaattg cgcagccaaa cactgcctcc aaaattgttc taatagatac
     1981 aattgaaaac accgcagctt gctgcggtgt ttttccgttg cgctaaccca aggaatccat
     2041 catgcaaacc aaagatcgca tcaaccaaga catgaaagac gccatgcgcg ccaaagaaac
     2101 ggaaaagctc tctaccatac gcatgctcat ggctgccatc aaacaagttg aagtggatga
     2161 acgcgttgaa gtagacgaca cccgcatcat tgacatcgtg accaaaatga tcaaacaacg
     2221 tcacgactct gtcaaaattt accgcgacaa caaccacaat gaagctgctg acaaagagca
     2281 agctgaaatt gatttgttgc acaattactt gccagcacaa ttgagtgaag ctgaagtcaa
     2341 agcggaaatt gccgctgccg ttgcagcgac aggtgcaagt tctatggcac aaatgggtca
     2401 agtcatggcc attttgaaac ccaaactcaa cggcgttgcc gacatgggtc aagtttctgc
     2461 atgggttaaa gccgcgctga attcatgata cccagcgatt tcatagacga attattgcaa
     2521 aaagtggaca t
//
LOCUS       DAOMVK010000336         2511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_12331_length_2511_cov_1.815961, whole genome shotgun sequence.
ACCESSION   DAOMVK010000336 DAOMVK010000000
VERSION     DAOMVK010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12331_length_2511_cov_1.815961"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..937
                     /locus_tag="PK856_09840"
     CDS             2..937
                     /locus_tag="PK856_09840"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HRL02708.1"
                     /translation="MGYALLFRIASGEMLGKDQPVILQLLDLPQAQNAVKGVMMELQD
                     CAFPLLAGMVATDDPEVAFKDAQVAILVGARPRSKGMERADLLAANAEIFTVQGAALN
                     KVADRGVKVLVVGNPANTNAYIAMKSAPDLNPNNFTAMLRLDHNRALSQVAEKTGKAV
                     GDIQKLCVWGNHSPTMYADYRFATINGENVKDLINDQEWNANEFLPTVGKRGAAIIEA
                     RGLSSAASAANAAIDHIHDWWLGTDGAWVTMGIPSDGSYGIPEGTMFGFPVTCANGKY
                     EIVQGLEIDAFSQERINITLAELEEEKAGVAHLLK"
     gene            complement(993..1340)
                     /locus_tag="PK856_09845"
     CDS             complement(993..1340)
                     /locus_tag="PK856_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02709.1"
                     /translation="MRSAILEEIKGKPGVRKMVGDIENVKIDLSQYPPVSCEDKKATS
                     WSDFVFKHHSKGCFRFSLGVQSDTLKTVKISTDYDTNKLIGERFSYVMLCQKPGEVAA
                     KDNGMPREYLNCE"
     gene            complement(1418..>1841)
                     /locus_tag="PK856_09850"
     CDS             complement(1418..>1841)
                     /locus_tag="PK856_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRL02710.1"
                     /translation="QQALSVVKAPVASLQAADIDRQVVRAAIANAHQAGVDDVLAFKV
                     QDVIKATAAAETGVLVSNPPYGIRLEDEHFLRGFYPQLSSVMKQQFHGWQVALISADM
                     GLPSGLRLKPARKIPMFNGKLDCRLFIFKMVQGSNRGE"
     assembly_gap    1842..1851
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1852..2509)
                     /locus_tag="PK856_09855"
     CDS             complement(<1852..2509)
                     /locus_tag="PK856_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HRL02711.1"
                     /translation="MEEVLVGELNALALQHIHAVDGGVDCVGSWEQVYQINLHSRVAS
                     RVLIELIRGACAREDQMYDLAKKVVWTNWFAVTDTIKMNVTGKRTPFKRIDILGLKIK
                     DAVCDVFRDACGKRPSVDKMFPDVRIHAFVEGTQARIMLDTSGEALFKRGYRDETGEA
                     PLRENLAAGLLMLAGYDGTQAFIDPMCGSGTIAIEAALLAKNQAPGLYRHFGFEKLKG
                     F"
BASE COUNT          673 a          658 c          513 g          657 t
ORIGIN      
        1 aattggctat gccttattgt tccgcatcgc cagtggcgaa atgttgggca aagaccaacc
       61 agtgattttg caattattgg atttgccaca agcacaaaat gccgttaaag gcgtgatgat
      121 ggaattgcaa gactgtgcat tccctttgtt ggcaggcatg gttgcaactg acgacccaga
      181 agttgccttt aaagatgcac aagttgccat cttggttggt gctcgcccac gttctaaagg
      241 catggagcgc gctgatttgt tggccgccaa tgctgaaatc tttactgttc aaggcgcggc
      301 tttgaataaa gttgctgatc gcggcgttaa agtattggtt gttggcaacc ctgcaaacac
      361 caatgcatac attgccatga aatcggctcc tgatttgaac cctaacaatt tcactgccat
      421 gttacgctta gaccacaacc gtgcgttgag ccaagtggct gagaaaactg gcaaagccgt
      481 tggcgacatc caaaaattgt gtgtttgggg caaccacagc ccgaccatgt acgctgacta
      541 ccgttttgca accatcaacg gtgaaaatgt taaagacttg attaacgatc aagaatggaa
      601 tgccaacgaa ttcttaccta cagtaggcaa acgtggcgca gccatcattg aagcccgtgg
      661 cttgtcttct gcggcttctg ctgccaacgc agccatcgac cacatccacg actggtggtt
      721 gggtactgac ggcgcttggg taaccatggg cattccttca gatggctctt acggcatccc
      781 tgaaggcacc atgttcggct tccctgtgac ttgcgctaac ggcaaatacg aaatcgttca
      841 aggcttggaa atcgatgcat tcagtcaaga acgcatcaac atcactttgg ccgaattgga
      901 agaagaaaaa gcgggcgttg ctcacttgtt gaaataacct cttaagtttc acaaaaaaag
      961 ccgcttaatt aagcggcttt tttatttgga tgttattcgc aattcagata ttcacgcggc
     1021 atgccattgt ctttggctgc cacttctcca ggcttttggc acaacatcac atatgaaaac
     1081 cgctcaccaa ttaatttatt ggtatcgtaa tcagtagaaa ttttcactgt tttcaatgta
     1141 tcactttgca cacccagtga aaatctaaaa cagcctttac tgtggtgttt aaaaacaaaa
     1201 tcactccaac tagtggcttt cttatcctca cagctgactg gtgggtattg gctcaagtca
     1261 attttgacat tttctatgtc acccaccatt tttctcacac caggcttgcc tttaatttct
     1321 tccaaaatgg ctgagcgcac ttgcaaatac tgatagccac aatagcctgc atatgcaaat
     1381 gctgcaaaca caaaattaat catcccccgt aaagtcatca ctccccccta tttgatccct
     1441 gtaccatttt gaatataaac aaacggcaat ccaatttgcc attgaacatt ggaattttgc
     1501 gtgcgggttt taaacgcaat ccgctcggca aacccatatc agcactgatt aaagccactt
     1561 gccagccgtg aaactgttgt ttcattacgc tgcttaattg tgggtaaaat ccacgtaaaa
     1621 aatgctcatc ttccaaacga atgccataag gtggattgga caccaacaca cctgtttccg
     1681 ccgccgcagt ggccttgatg acatcttgca ccttaaaggc caaaacatcg tctacgcctg
     1741 cttggtgagc attggcaatc gctgctctta ctacttgtct gtcgatatca gcggcctgta
     1801 aacttgccac cggcgctttt accacagaca aagcctgttg cnnnnnnnnn ngaaaaccct
     1861 ttaacttttc aaaaccaaaa tgacggtaca aaccaggcgc ttggtttttc gccagcaaag
     1921 ccgcctcaat cgcaatggtg ccactgccgc acattgggtc gataaaggct tgagtgccat
     1981 cgtatcctgc caacatcaat aagccggccg ccagattttc acgcaatggc gcctcacctg
     2041 tttcatcacg gtaaccgcgt ttgaacaaag cttcaccact ggtgtctagc atgatgcgtg
     2101 cctgcgtgcc ctcaacaaag gcatgaatgc gcacatcagg aaacattttg tccacgcttg
     2161 ggcgcttgcc acaagcatcg cgaaacacat cacacaccgc atctttgatt ttcaaaccca
     2221 aaatatcaat gcgtttaaac ggtgtgcgct tgcctgtcac attcattttg atggtgtcag
     2281 tcaccgcaaa ccaattggtc cacaccactt ttttagccaa atcgtacatt tggtcttcac
     2341 gcgcacatgc gccgcgtatc aactcaatca acacccgcga agccacgcga gaatgcaggt
     2401 taatttggta gacttgctcc caacttccca cgcaatccac gccaccatca acagcgtgta
     2461 tgtgttgcaa tgccaaggca ttcaactcac caaccaaaac ctcttccaaa c
//
LOCUS       DAOMVK010000337         2509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_12345_length_2509_cov_2.338223, whole genome shotgun sequence.
ACCESSION   DAOMVK010000337 DAOMVK010000000
VERSION     DAOMVK010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12345_length_2509_cov_2.338223"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="PK856_09860"
     CDS             <1..643
                     /locus_tag="PK856_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRL02712.1"
                     /translation="AVAVGDEIADKMIEALQAAMSKLKFGVHSDKTNDFGPVITAEHK
                     AKVVGHIQSAAEQGAKVLVDGSAAAPAGYENGFFVGATLIDGVTKEMSSYQAEIFGPV
                     LQVLRVQTMAEAMQLINDHEYGNGTCIYTRDGEAARYFADNIQVGMVGINIPLPVPVA
                     YQSFGGWKRSLFGDLFMYGPDGVRFFTRRKTITQRWPSSEVRESAQFSMPTLG"
     gene            762..1406
                     /locus_tag="PK856_09865"
     CDS             762..1406
                     /locus_tag="PK856_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02713.1"
                     /translation="MNKKEWVYFAKGMLVCMVSVACLLGLSLWLLIYVNRDLQQEMSF
                     EQVLNLILVTIIALMFPLIQYGQARWLWHTRHVPAGQQPEWMSEKTAHLPKIAKPWQQ
                     RLLEVGLQVGAMLLLLWLFGAYATQQRLVGIANEYDISSGTYLLCVLVLAAVPIAVLA
                     WLVKMVREMVASKWDKQGLRYQLWRGWLWAYVLAFGLCVYLIQMMGVMIVRYLE"
     gene            1757..1987
                     /locus_tag="PK856_09870"
     CDS             1757..1987
                     /locus_tag="PK856_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02714.1"
                     /translation="MFTQTHIAQANAIESQIEMELMFLSEATQLSCLELIMGLEAEAR
                     ITYLNQYMNKVAALVDTASRVEMAESVYLMAA"
     assembly_gap    2199..2208
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2209..>2509)
                     /locus_tag="PK856_09875"
     CDS             complement(<2209..>2509)
                     /locus_tag="PK856_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HRL02715.1"
                     /translation="FALFFFAFTTIVAYYYIAETNIAYLTRQNEKLRSTLMLVLKLAM
                     IVMVVFGCMRTAELAWGLGDIGVGLMAWLNIIAILLLQKPALQSLKDYERQQKEG"
BASE COUNT          631 a          528 c          677 g          663 t
ORIGIN      
        1 ggccgtggcc gtgggcgacg agattgccga caagatgata gaggccttac aggccgccat
       61 gagcaaattg aaattcggcg tgcattcaga caaaaccaac gattttggcc ccgtgattac
      121 cgctgaacac aaagccaaag tggtgggcca catccaatct gctgccgagc aaggcgcaaa
      181 agtgttggta gacggctctg cggctgcgcc agcaggttat gaaaacggct tctttgtcgg
      241 tgcaaccttg attgatggcg tgaccaaaga aatgagcagc taccaagccg aaatctttgg
      301 cccagtattg caagtcttgc gtgtgcaaac catggctgag gcgatgcaat tgattaacga
      361 ccacgaatac ggcaatggca cgtgcatcta cacccgtgac ggtgaagcgg cgcgctattt
      421 cgccgacaac attcaagtgg gcatggtggg catcaacatt ccattgcctg tgcctgtggc
      481 ttaccaaagc tttggcggtt ggaaacgctc cttgtttggt gatttgttca tgtacggtcc
      541 cgatggtgtg cgcttcttca cccgtcgcaa aaccattacc caacgctggc caagctcaga
      601 agtgcgtgaa agtgcgcagt tctccatgcc tactttgggt taatgcgctg tattgagtgt
      661 gtgtaggaat cagcggcctg taaagtctta caggccgctt ttattgtgct cagtacagcc
      721 ttatgtttac actgtgggca agataacaga atctgtgtgg tatgaataaa aaagaatggg
      781 tgtatttcgc caaaggcatg ttggtgtgca tggtgagcgt ggcctgcctc ttgggcttga
      841 gtttgtggtt actcatctat gtgaatcggg atttgcagca agagatgagt tttgagcaag
      901 tgctgaatct gattttggtg accatcatcg ccttgatgtt tcccttgatt caatatgggc
      961 aagccagatg gttgtggcac acgcggcatg tgccagcagg gcagcagcct gaatggatgt
     1021 cggagaaaac cgcgcacttg cccaaaatcg ctaaaccttg gcaacagcgt ttgttggaag
     1081 tgggactgca agtgggcgcc atgttgctgt tgttgtggct gtttggtgcg tatgccacac
     1141 agcagcgttt ggtgggcatc gccaatgagt atgacatcag ctctggcacc tatttattgt
     1201 gtgtgttggt gttggcggcc gtgcccattg cggtgttggc atggctggtt aagatggtga
     1261 gggaaatggt tgccagcaag tgggacaagc aagggctgcg ctatcaattg tggcgggggt
     1321 ggttatgggc ttatgtgctg gcatttggtt tgtgcgtcta cctcattcaa atgatgggag
     1381 tgatgatagt gcggtatttg gagtaagcgc atcgtgtgtt gacttgataa gcggcctgta
     1441 aatgtttaca ggccgctttt aaatctgaaa acgggacaca gataggcatt tggctgcgta
     1501 tgattgccca aaaaggtttg ataagtgcag atgggtttgt tcggtgcggc caaattttaa
     1561 ccgcgaagag attggaaaat agaataattc atgcagatgg gatgtaccgt gagtgcagta
     1621 ttgatggggg tgttaagagc gttagtgttg atttataaga taaaagcttt aataaactgc
     1681 agtgccaaaa tttatttggc atatctattg ctcaatatta ttcacaaacc aacacactga
     1741 tttaaggaac accgccatgt ttacccaaac acacatcgct caagccaatg ccatcgaatc
     1801 tcaaatcgaa atggaattga tgtttttgtc tgaagccacc caattgagct gtttggaatt
     1861 gatcatgggt ttggaagccg aagcgcgcat cacctacttg aaccaataca tgaacaaagt
     1921 ggccgcgttg gtggacacag cgagccgtgt agaaatggcg gaatcggtat acttgatggc
     1981 agcttgatag cgccagcagc agatgccaaa cattcagtgt gtgaacctat catctgctga
     2041 caaatgaaaa acccttggtt tgaaccaagg gttttttaac acctaccgat taagggatga
     2101 agcggtcctc aatcggggtt ttttgtttgc ggaactcttc accaatttcc ttccaaatcg
     2161 gcgcattgtt aacgccattt tcttctggta cataaattnn nnnnnnnntt gccttctttt
     2221 tgttggcgct cgtaatcttt gagcgattgc agtgcgggtt tttgcagcag caagatggcg
     2281 atgatgttca gccaagccat caagcccacg cctatatcgc ccaagcccca agccaattca
     2341 gcagtacgca tgcaaccgaa taccaccatc acgatcattg ccaatttcaa taccaacatc
     2401 aaagttgagc gcaatttttc attttgacgg gtcaagtaag caatgttggt ttcagcgatg
     2461 tagtaatagg ccacgatggt ggtgaaagcg aagaagaaca aggcaaaag
//
LOCUS       DAOMVK010000338         2505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_12372_length_2505_cov_2.480408, whole genome shotgun sequence.
ACCESSION   DAOMVK010000338 DAOMVK010000000
VERSION     DAOMVK010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12372_length_2505_cov_2.480408"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="PK856_09880"
     CDS             complement(<1..704)
                     /locus_tag="PK856_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRL02716.1"
                     /translation="MKTRAAVAWAPNEPLKIEELDLMPPQKGEVLVRIVATGVCHTDA
                     YTLSGQDSEGVFPCVLGHEGAGIVEAVGEGVTSVAVGDHVIPLYTAECGQCKFCLSGK
                     TNLCSSVRETQGKGLMPDGTVRFYKDGQPIYHYMGCSTFSEYTVVAEVSLAKIQPEAD
                     LNEVCLLGCGVTTGMGAVMNTAKVKRGDTVAIFGLGGIGLAAIIGARMSGAKRIIGID
                     TNPSKFAMAEKLGATD"
     gene            1088..1243
                     /locus_tag="PK856_09885"
     CDS             1088..1243
                     /locus_tag="PK856_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02717.1"
                     /translation="MDVFAELLKSDVGILSSITILMCITISVVFMLWTRKQIRKDEAK
                     HRQNQSE"
     gene            complement(1388..1726)
                     /locus_tag="PK856_09890"
     CDS             complement(1388..1726)
                     /locus_tag="PK856_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRL02718.1"
                     /translation="MKKVEAVIKPFKLDEVREALTEVGITGMTVTEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKIKLELVLPDALVEQAVETIIQAAHSGKIGDGKIFVSNIEQAIRI
                     RTGERDDAAV"
     gene            1917..>2309
                     /locus_tag="PK856_09895"
     CDS             1917..>2309
                     /locus_tag="PK856_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HRL02719.1"
                     /translation="MPEVLSLRGQTALSSFRIDQLNQQARKLHLPEQAQLSSEYWYFI
                     HSDAVLSANDVHRLCDLLTAEHPTTTERSEQLCLITPRLGTISPWSSKATDIAHNCGI
                     NAPGLRIERATALWLSTDLDAAQLTAWQG"
     assembly_gap    2310..2319
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2320..>2505
                     /locus_tag="PK856_09900"
     CDS             <2320..>2505
                     /locus_tag="PK856_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02720.1"
                     /translation="LHDRMTESVMTDLADARQLFERIQDESFASVDVLGGGRAALIAA
                     NTRMGLALSEDEIDYLV"
BASE COUNT          625 a          655 c          537 g          678 t
ORIGIN      
        1 atgtcggttg cgcccaattt ttctgccatc gcaaatttgc ttggattggt gtctatgccg
       61 ataatgcgtt tcgcacctga catgcgcgcg cctatgatgg ctgccaaacc aatgccgccc
      121 aagccaaaaa tcgccacggt atcgccgcgt ttcactttgg cggtgttcat caccgcgccc
      181 atgcctgtgg tgacaccaca gccaagcagg cacacttcgt tcaaatcggc ttcaggttgg
      241 atttttgcca acgacacttc tgccaccacg gtgtattcag aaaacgttga gcagcccatg
      301 tagtggtaaa tcggttggcc gtctttgtaa aaacgcaccg taccatcggg catcaagccc
      361 ttgccttggg tttcgcgcac agacgaacac aaattggttt tgccagacaa acagaattta
      421 cattgtccac attctgctgt gtaaagcgga atcacatggt cacccaccgc caccgaagtc
      481 acgccctctc ccacggcttc cacgatgccc gcgccctcat ggcccaacac acacgggaac
      541 acaccttcag aatcttgacc agacagggta tacgcatcgg tatggcacac acccgtggcc
      601 acaatgcgca ccaatacctc gcctttttgt ggcggcatca aatccaattc ttcaattttc
      661 aaaggttcat tcggtgccca agccacggct gcacgggttt taatcatttg catgtcgcta
      721 ctgtcctttt ctgtgtttct aaaaaagttt gtttattgtg ctgtcatcat aacagatggc
      781 aagcactgtg gacgcaattc cacccgcttc agtcgcctct cgtccctctt gcgatattaa
      841 tgagcaccta actagtcgca caagcaaagc atagccacca tgagaagtgc cttagtcaat
      901 ctcaaacagc attatcccta ataaaatgat ttgtatgagg cttttggatt actggcttca
      961 gttgacctgc atcaaaccca catctaggtt ttcatcttaa gatgggacgc attaaaaata
     1021 acaaatacat aagcatcgtc acaatggcat cgtgtttcac ctgagttttt gagaaagcga
     1081 gactgtaatg gatgtttttg cagaattatt aaaatcggat gtgggcattt tatccagcat
     1141 cacgatactg atgtgcatca cgatttcagt ggtgtttatg ctgtggacga gaaaacaaat
     1201 acgcaaagat gaagccaagc accgacaaaa ccaatctgaa tagcgctttt atggcttaac
     1261 cattcctctt gatgtgtgtg tttgggtgtg atgcttatca cacccttttt ttggctgttg
     1321 ttccaccaca aaaaaaccca aatcaacatc gctgatttgg gttatgtgtt tgctatgggt
     1381 gtaaaaatca aacggcagcg tcgtcgcgtt cgcccgtgcg tatgcgtatt gcctgctcaa
     1441 tattagacac aaagatttta ccatcaccaa ttttgcctga gtgagcggcc tgtatgatgg
     1501 tttccacagc ttgctcaacc aaagcgtctg gcaacaccaa ttccaatttg atttttggta
     1561 aaaaatccac tgcatattcc gcgccacggt aaatttcggt atggcctttt tggcgaccaa
     1621 aacctttgac ttcggtcact gtcatgcctg tgatacccac ctcagtcaag gcttcgcgca
     1681 cttcgtccaa tttaaatggc ttgattaccg cttcaacttt tttcatactc aaactcccaa
     1741 aacccaaaaa aattctactt tgtcacactt taaaccaaag ctgcattatt tgccaagcct
     1801 tgccaaatga gaattttcgg cgtaatggtc taggattatt aaggcaataa accacgattt
     1861 aaggtatcat gttgcgctgt cacttttacc cctaattatc agctttgcga gacatcatgc
     1921 ctgaagtgct atcgttacgc ggtcaaaccg cgctgtcgtc attccgtata gaccaactga
     1981 atcaacaagc gcgcaaattg cacctgcctg aacaagccca attgagcagt gagtattggt
     2041 atttcatcca cagcgatgct gtgttgtcag caaacgatgt ccatcgtttg tgtgatttat
     2101 tgactgccga acacccaacc accactgagc gcagcgaaca actgtgcttg attacccctc
     2161 gcttaggcac catttcccct tggtcgtcca aagctactga catcgcccac aactgtggca
     2221 tcaatgcgcc tggtttgcgc atcgagcgcg ccaccgcatt gtggctctct actgatttgg
     2281 atgcggctca attgaccgca tggcagggtn nnnnnnnnnt gttgcacgac cgcatgaccg
     2341 aatcggtgat gaccgatttg gccgatgcgc gccaactgtt tgagcgcatc caagacgaaa
     2401 gctttgcttc ggtagatgtg ttgggcggtg gtcgcgctgc tttgatagcc gccaacaccc
     2461 gcatgggttt ggccttgtct gaagacgaaa tcgattactt ggtgg
//
LOCUS       DAOMVK010000339         2504 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_12381_length_2504_cov_2.978359, whole genome shotgun sequence.
ACCESSION   DAOMVK010000339 DAOMVK010000000
VERSION     DAOMVK010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12381_length_2504_cov_2.978359"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="PK856_09905"
     CDS             <1..387
                     /locus_tag="PK856_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRL02721.1"
                     /translation="AAEWLTQVEIDAGRINDLPRTFSGGMQQRLQIARNLISKPRLVF
                     MDEPTSSLDVSVQAKLLDLIRSLVRKYQLSIILVTHDLAVARLLADRLLVMQRSHVVE
                     SGLTDQILDDPQHPYTQLLVSSILQA"
     gene            401..1114
                     /gene="phnL"
                     /locus_tag="PK856_09910"
     CDS             401..1114
                     /gene="phnL"
                     /locus_tag="PK856_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HRL02722.1"
                     /translation="MMTATRIQARSLNKQFTLHHQNGVQLDVLTDLEFDAKSGECLIF
                     SGRSGSGKSTLLKMIYGNYLATSGSLQVKHGDEWVEMVAAAPRSVVELRRHTIGYVSQ
                     FLKTIPRVSTLNLVMEPALERGWSVEQARERAENLLSRLNIPERLWHLAPSTFSGGEQ
                     QRVNIARGFMVAWPVLLLDEPTASLDETNRAVVVELMQEAKAGGAAMVGIFHDAEVRQ
                     SIGDKQFNVSANEVSSHVQ"
     gene            1104..2246
                     /gene="phnM"
                     /locus_tag="PK856_09915"
     CDS             1104..2246
                     /gene="phnM"
                     /locus_tag="PK856_09915"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HRL02723.1"
                     /translation="MSNEIILNNVRMVTADEVVLGSMLIENGIIKAIDTQVSALPQAQ
                     DLHGDYVIPGLVELHTDNLEKFMNPRPGVDWPSMSAIVGHDNQVISSGITTVFDALSV
                     GDISPKGDRLANLLPMLEAITHSQQEGITRAEHKIHLRCEVAHPKTHERFMELNDNAY
                     VGLVSIMDHSPGQRQFAQLDKYREYYQGKYGMNAAQMDEFAEKQIRHAETYSQPNRLS
                     ITEHCRSKGLALASHDDATAAHVDESHALGMEIAEFPTTEEAASLSHQKGLKVLMGAP
                     NVVRGGSHSGNISAHFLAQLGLLDILSSDYYPSSLLQAAFMLTQEHIGYSLPQAIRTV
                     SKNPAESVHLHDRGEIAIGKIADLVQVKHTVLPMVQQVWKQGKRVF"
     gene            2246..>2504
                     /locus_tag="PK856_09920"
     CDS             2246..>2504
                     /locus_tag="PK856_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02724.1"
                     /translation="MFTGRLIYLMGPSGAGKDSLIALIKHKAELPLLLPSRYITRPVN
                     AAELEQHQFMTPETFATAEQAGILLARGAPMAMLMAWVWMCT"
BASE COUNT          616 a          650 c          665 g          573 t
ORIGIN      
        1 gccgccgaat ggctcacgca agtggaaatc gatgcagggc gcatcaatga tttgccgcgc
       61 acgttttcag gcggcatgca gcagcgtttg caaatcgccc gcaatttgat ttccaagcca
      121 cgcctggtgt tcatggatga gcccaccagc tctttggatg tgtcggtaca ggcgaaatta
      181 ttggatttga ttcgctcttt ggtacgcaaa tatcaattgt ccatcatctt ggtgacgcac
      241 gatttggcgg tggcgcgttt gctggccgac cgtttgttgg tgatgcagcg ctcgcacgtg
      301 gtggaaagcg gccttaccga ccaaattctc gacgacccac aacaccctta cacgcaatta
      361 ttggtttctt cgatattaca ggcttaaaaa cagagaacaa atgatgactg ccacacgcat
      421 acaagcacgc agcttgaaca agcaatttac cttgcaccac caaaacgggg tgcaattgga
      481 tgtgttaacc gatttggaat tcgatgccaa aagtggcgag tgtttgattt tttcaggccg
      541 ctcaggcagt ggcaaatcga ctttattgaa aatgatatac ggcaactact tggccacttc
      601 aggctctttg caagtgaaac acggcgacga atgggtagaa atggttgctg ccgcgccgcg
      661 cagcgtggtg gaattgcgcc gccacaccat aggctatgtg agccaatttt tgaaaaccat
      721 cccgcgcgta tcgacattga atttggtgat ggagccagct ttagagcgcg gttggagcgt
      781 ggagcaagcg cgtgaacgcg ctgaaaacct gctcagccgc ttgaacattc ctgagcgctt
      841 gtggcatttg gcaccgagca ccttctcagg tggtgaacaa cagcgcgtga acatcgctcg
      901 cggctttatg gtggcatggc ccgtgttgtt attggatgaa cccaccgcgt ctttggacga
      961 aactaaccgc gccgtggtgg tggaattgat gcaagaagcc aaagcaggcg gtgcggcgat
     1021 ggtgggcatt ttccacgatg ccgaagtgcg ccaaagcata ggcgacaaac aattcaatgt
     1081 cagtgccaac gaggtatcca gccatgtcca atgaaatcat tttaaacaac gtgcgcatgg
     1141 tcactgccga tgaggtggtg ctgggttcca tgttgattga aaacggcatc atcaaagcca
     1201 tcgataccca agtgtctgct ctgccgcaag cgcaagattt gcatggtgac tatgtgatac
     1261 caggcttggt ggagttgcac accgacaatt tggaaaaatt catgaacccg cgccctggtg
     1321 tggattggcc gtctatgtcg gcaatcgtcg ggcatgacaa ccaagtcatc agctcaggca
     1381 tcaccacggt gttcgacgct ttgtcagtgg gtgatatcag ccccaaaggc gaccgattgg
     1441 ccaatttgtt gcccatgctc gaagccatta cccacagcca acaagaaggc attacccgcg
     1501 ccgagcacaa aatccatttg cgctgcgaag tggcccaccc gaaaacgcac gagcgtttca
     1561 tggaattgaa cgacaatgcc tatgtgggct tggtgtccat catggaccat tcacctgggc
     1621 aacgccaatt cgcccagctc gacaaatacc gcgagtatta ccaaggcaaa tacggcatga
     1681 acgcggctca aatggatgaa tttgccgaaa aacaaatccg ccatgccgaa acctacagtc
     1741 agcccaaccg cttgtcgatt accgagcatt gccgcagcaa aggcttggcc ttggccagcc
     1801 acgacgatgc caccgccgcg catgtggatg aatcgcacgc tttgggcatg gagattgccg
     1861 agtttcccac cacggaagaa gccgccagtt tgtcgcatca aaaaggcttg aaagtgttga
     1921 tgggcgcgcc caacgtggtg cgcggtggtt cacattcggg taatatttca gctcatttct
     1981 tggcacaact gggcttgttg gatatcttgt ccagcgatta ctatccgagc agtttgttac
     2041 aggccgcatt catgctcacg caagaacaca taggctatag cttgccgcaa gcgatacgca
     2101 cagtcagcaa aaaccctgct gaatcggtgc atttgcatga ccgcggtgaa atcgccattg
     2161 gcaaaatcgc cgatttggtg caagtcaaac acactgtttt gcctatggtg caacaggtgt
     2221 ggaaacaagg aaagcgggta ttttaatgtt tacaggccgc ttgatttacc tgatggggcc
     2281 ttctggcgcg ggcaaagaca gcttgattgc gctcatcaag cacaaagccg aattgccctt
     2341 gctgttgccc agccgttaca tcacccgacc tgtcaacgcc gccgaattgg agcaacacca
     2401 attcatgacg cctgaaacct ttgccaccgc cgagcaagca ggcattttgc ttgctcgtgg
     2461 cgcgccaatg gccatgctta tggcttgggt ttggatgtgt acac
//
LOCUS       DAOMVK010000340         2499 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_12424_length_2499_cov_2.432488, whole genome shotgun sequence.
ACCESSION   DAOMVK010000340 DAOMVK010000000
VERSION     DAOMVK010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12424_length_2499_cov_2.432488"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PK856_09925"
     CDS             complement(<1..314)
                     /locus_tag="PK856_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02725.1"
                     /translation="MRLPFLTILLACAALPLTAVAEKVVDPIKEMQQASHTRKTQTIK
                     GVLSVYWQEQKIRLAMNSDLASRKQLYRNTPNQKRIEHTRADMKFVDLHWRGSQTQLQ
                     QA"
     gene            complement(325..1413)
                     /locus_tag="PK856_09930"
     CDS             complement(325..1413)
                     /locus_tag="PK856_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02726.1"
                     /translation="MTSLRYFGVLLVLASAACQPNQQNQAASQPTTHANHAASAPKSP
                     LPPQCDNIPLQMSAITANSDLQSLSVLNLALKRCVPLVNMETRKLWLDASWDMYQRFL
                     KLGGDSKTAMVWDEFSGILEGYPDESSEDKQARLQRQAELWPRLTPRMQQMSLWQGKE
                     YIDNYYIGEGEMALRRHPQFVTDIFSPYLPEDEQVFTRQLAKENTTLTTNDAALVIPW
                     SEVSNRALFWERYLKRYPDSPYAKQAEYLFQWYQAILFQGLDNTPVLEFDEKQLNISA
                     DAMAVHQLLSTKTDSRLAAKSSAFIQWLQQQQQHWQQLSPDERTGFDKRLQSEQKKLK
                     VAFPVPVSAGKNSHFDCFTDALCHPIKG"
     gene            complement(1530..>2499)
                     /locus_tag="PK856_09935"
     CDS             complement(1530..>2499)
                     /locus_tag="PK856_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRL02727.1"
                     /translation="YKKFGMNVDPKNILITTGSQQALDLLGKVFLNKGDCVCLERPSY
                     LGAIQAFQMFQPKFQEVDLTTTGIDLPELEKQMAATQAKFMYAIPNFQNPTGLTYSLE
                     SRQNLADYLKRSGNVLVEDDPYGELRFIGEHLPSVYSFAPDNVILLGSFSKVCAPGMR
                     IGWMVANDTVMERLVTAKQASDLHTPILTQRILAQYLADNSLDEHIKTIQAAYFEQRQ
                     AMVEALHQYMPEGVTFTEPEGGMFLWVSLPKNCPAMSLFPIAVKHKVAFVPGEPFSTE
                     GKTSYDIRMSFCTVDAATIVEGVKRLATAIAELTNQNQTDNHTVTI"
BASE COUNT          585 a          566 c          653 g          695 t
ORIGIN      
        1 aaagcttgtt gcaattgcgt ttggctgccg cgccaatgca aatccacaaa cttcatgtct
       61 gcacgggtgt gctcaattct tttttgattg ggagtgttgc gatacagctg cttccgcgag
      121 gccaaatcac tgttcattgc caaacgtatt ttctgttcct gccaatagac cgataaaaca
      181 cccttaatgg tttgggtttt gcgggtgtga ctggcttgct gcatttcttt gatggggtct
      241 accacttttt ctgccacggc ggttaaaggc aaggcggcgc aggcaaggag gatggtgagg
      301 aatggaaggc gcatattgtc ctttctagcc tttaatgggg tggcataagg catcggtaaa
      361 gcaatcaaaa tggctgtttt tgccagcaga cacaggaaca gggaaagcca ccttcagttt
      421 tttctgttcg ctttgcaggc gtttatcaaa accagtacgc tcatctgggc ttaattgctg
      481 ccagtgttgc tgctgttgtt gtagccattg aatgaaggcg ctggatttgg ctgccaagcg
      541 gctgtcggtt ttggtgctga gcaattggtg tacggccatg gcatcggcgc tgatgttcaa
      601 ttgtttttca tcaaattcca acacaggcgt gttgtccaag ccttgaaaca aaatggcttg
      661 gtaccattga aacaagtatt cggcttgttt ggcatagggg ctatcgggat agcgtttcag
      721 gtagcgctcc caaaacaagg cgcgattgga cacttctgac caaggaatca ccaaggctgc
      781 atcattggtg gtgagggtgg tgttttcttt ggccaattgg cgcgtgaata cctgttcatc
      841 ctctggcaag taaggggaga aaatgtcggt gacaaactgt gggtggcgcc tcaaggccat
      901 ttcgccttcg cctatgtaat aattgtctat gtattccttg ccttgccaca aagacatttg
      961 ctgcatgcgt ggggtcaggc gtggccacaa ttcggcttgg cgttgcaaac gcgcttgttt
     1021 gtcttccgag ctttcatcag gatagccttc tagaatgcct gaaaattcat cccaaaccat
     1081 tgcggttttg ctgtcaccac cgagtttgag aaagcgttgg tacatgtccc aactggcatc
     1141 caaccacagt tttcgtgtct ccatgttcac caaaggcacg cagcgtttca gcgccaaatt
     1201 gaggacagac agcgactgca aatcgctgtt ggctgtgatg gcgctcattt gcaaagggat
     1261 gttgtcacat tggggtggaa gcgggctttt gggggcagag gcagcatgat tggcgtgcgt
     1321 tgttggctgg ctggcagctt gattttgctg atttggttgg caggcggcac ttgccaacac
     1381 caaaagaacg ccaaagtaac gcaaagaagt catcgccttt aacccatatt catagacctt
     1441 ggtatcttaa caaaaacgcc agtgtcttac agtgaaataa gacaggcgtc ctgtaaactt
     1501 tcaggacgcc tgtgttgcat tgtgcaaact caaatggtca cggtgtggtt atcggtttga
     1561 ttttgattgg ttaattccgc gatggctgtg gccaagcgtt tcacgccttc aacaatggtg
     1621 gcggcatcca cggtacagaa actcatgcgg atgtcgtaag aggttttgcc ttcagtagag
     1681 aacggttcac ctggtacaaa cgccactttg tgtttcactg caatcgggaa caagctcatg
     1741 gcagggcagt ttttcggcaa gcttacccac aagaacatgc cgccttcagg ctcggtgaag
     1801 gtcacgcctt caggcatgta ttggtgcaaa gcctctacca tggcttggcg ttgctcaaaa
     1861 taagcggcct gtatggtttt gatgtgctca tccaatgagt tatcggccaa gtattgtgcc
     1921 aaaatgcgtt gggttaaaat cggtgtgtgc aaatcagagg cttgtttggc cgttaccaaa
     1981 cgctccatta ccgtgtcatt ggccaccatc caaccgatgc gcataccagg cgcgcacact
     2041 ttggagaatg agcccaataa aatcacattg tcaggcgcga atgaatacac gctgggcaaa
     2101 tgctcgccta tgaagcgcaa ttccccatac ggatcgtctt ccaccaacac attgccgctg
     2161 cgttttaagt agtcggccaa gttttgacgg ctttccaagc tgtaagtcaa acctgttggg
     2221 ttttggaaat tgggaatcgc atacatgaat ttggcttggg tggccgccat ttgtttttcc
     2281 aattcgggca aatcgatgcc tgtagtggtt aaatccactt cttgaaattt gggttggaac
     2341 atttggaagg cttggatggc gcccaaatag cttgggcgtt ccaaacacac gcaatcaccc
     2401 ttgttcaaga acactttgcc caataagtcc aaggcttgtt gtgaacctgt ggtaatcaaa
     2461 atgtttttgg gatcaacatt catgccgaat tttttgtaa
//
LOCUS       DAOMVK010000341         2497 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_12433_length_2497_cov_3.894349, whole genome shotgun sequence.
ACCESSION   DAOMVK010000341 DAOMVK010000000
VERSION     DAOMVK010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12433_length_2497_cov_3.894349"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2497
                     /gene="clpB"
                     /locus_tag="PK856_09940"
     CDS             <1..>2497
                     /gene="clpB"
                     /locus_tag="PK856_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HRL02728.1"
                     /translation="RNDNGYLEAVHVLKVLLDDFDGGSQSLLQQARVDVARLQGEVQT
                     MIHGLPKVTGQGGEILPSRELQNTLNLMDKAAAKRGDTFIASELFFVALLQENGATGK
                     LLKQAGASEASLNNAIDAIRGGQSVDNANAEDQREALKKYTLDLTQRARDGKLDPVIG
                     RDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNEEVPESLKGKRLLVL
                     DLASLIAGAKFRGEFEERLKAVLSDLSKDDGQTIIFIDEIHTLVGAGKADGAMDAGNM
                     LKPALARGELHCIGATTLDEYRQYIEKDAALERRFQKVMVNEPSVESTIAILRGLKER
                     YEIHHKVDITDPAIVAAAELSARYITDRFLPDKAIDLIDEAASLIKMESESMPESMDK
                     LNRRIIQLKIEEAALKKEDDDASKKRLELMQEELLDLQKQFSDLEEVWKAEKAVSQGS
                     AGIKEQIDQIKIKMAQLQREGKWEDLAKLQYEQLPKLEAQLQAAETNPAADGGNKLLR
                     TEVGAEEIAEIVSRMTGIPVAKMMQGEKEKLLNMESVLHQRVVGQDEAVTAVANAIRR
                     SRSGLSDPNKPYGSFLFLGPTGVGKTELCKTLAGFLFDSEDHLIRIDMSEYMEKHSVS
                     RLIGAPPGYVGYEEGGYLTEQVRRKPYSVVLLDEVEKAHPDVFNILLQVLDDGRLTDG
                     QGRTVDFKNTVIVMTSNIGSMHIQTLGETASYQDIKDAVMEDVKIHFRPELINRIDEV
                     VVFHSLGQAQIRSIANIQLKGLRKRLEGLDLHLQIDDAALDVLAEVGFDPVYGARPLK
                     RAIQAQIENPLAQKLLAGVYAPESTIMITAKDG"
BASE COUNT          720 a          591 c          664 g          522 t
ORIGIN      
        1 tgcgcaatga taacggctat ctggaagcgg tgcatgtctt aaaagtatta ctcgatgatt
       61 ttgatggcgg cagccagtct ttgttgcaac aagcccgtgt cgatgtggcg cgtttgcaag
      121 gcgaagtgca aaccatgata cacggcttgc ccaaagtaac aggccaaggc ggcgagattc
      181 tgcccagccg tgaattgcaa aacaccttaa atttaatgga caaagcagca gccaaacgtg
      241 gcgatacctt catcgccagc gagttgtttt ttgtggcttt gttacaagaa aacggcgcaa
      301 caggcaaatt attaaagcaa gcaggcgcat ccgaagcgag cctgaacaat gccattgatg
      361 ccatccgtgg aggacaaagc gtggacaacg ccaatgcaga agaccaacgt gaagcactga
      421 aaaaatacac cttagattta acccaacgtg cgcgtgacgg caagctagac cctgtcatcg
      481 gtcgtgacga tgaaattcgc cgcgccatac aagtattgca acgccgcacc aaaaacaacc
      541 ccgtgttaat cggtgaacct ggcgtgggta aaactgccat tgttgaaggt ttggcacagc
      601 gcatcgtcaa tgaagaagtg ccagaatcct taaaaggcaa acgcctattg gtattggatt
      661 tggccagctt gattgcgggc gccaaattcc gcggtgaatt tgaagaacgt ctgaaagcgg
      721 tattgagcga tttgtccaaa gacgatggcc aaaccatcat cttcatcgac gaaatccaca
      781 ctttagtggg agcaggcaaa gccgatggcg caatggatgc gggcaatatg ttgaaacctg
      841 cattggcccg tggcgagttg cactgcatag gcgcaaccac tttagacgaa taccgccaat
      901 acattgaaaa agatgcggct cttgagcgcc gtttccaaaa agtgatggtg aacgagccga
      961 gcgtggaatc cacgattgcg attttgcgcg gcttgaaaga gcgttatgaa atccaccaca
     1021 aagtggacat taccgaccct gccattgtcg cagcggccga attgtcggca cgttacatca
     1081 ccgaccgttt cttgcccgac aaagcgattg atttgataga cgaagcagcg tctttgatca
     1141 aaatggaaag cgaatccatg cccgaaagca tggacaagct caaccgccgc atcatccaat
     1201 tgaaaattga agaagcggcg ctgaaaaaag aggacgacga tgcatctaaa aaacgcttgg
     1261 aattgatgca agaagaattg ctggatttgc aaaagcaatt ctccgatttg gaagaagtgt
     1321 ggaaagccga aaaagccgtg tcacaaggtt cggcaggcat caaagagcaa attgaccaaa
     1381 tcaaaatcaa gatggcgcaa ttgcaacgcg aaggcaaatg ggaagacttg gccaagctgc
     1441 aatacgagca attgccaaaa ctggaagcac agttacaggc cgctgaaacc aaccctgctg
     1501 cggatggcgg caacaaattg ctgcgcaccg aagtgggcgc agaagagata gccgagatag
     1561 tcagccgcat gacgggcatt cctgtggcca aaatgatgca aggcgagaaa gaaaaattgc
     1621 tgaacatgga aagcgtgttg caccaacgtg tggtcggtca agacgaagcc gtgactgcgg
     1681 tggccaatgc catacgtcgt tcgcgctctg gcttatcaga cccgaacaaa ccgtatggca
     1741 gcttcttgtt cttaggccct accggtgtgg gtaaaaccga attgtgcaaa accttggcag
     1801 gctttttgtt tgacagcgaa gaccatttaa tccgcatcga catgtcggaa tacatggaaa
     1861 aacactcggt atcgcgctta atcggcgcgc ctccgggcta tgtgggctat gaagaaggcg
     1921 gctatttgac cgaacaagtg cgccgcaagc cttacagcgt agtgttgttg gatgaggtgg
     1981 aaaaagccca tccagatgtg ttcaacattt tgctgcaagt attggacgat ggccgcttaa
     2041 ccgatggcca aggccgaacg gttgatttta aaaacaccgt gattgtgatg acgtccaata
     2101 tcggcagcat gcacatccaa accttgggtg aaaccgcgtc ttaccaagac atcaaagacg
     2161 cagtgatgga agacgtgaaa atccacttcc gtcctgagtt gattaaccgc attgatgagg
     2221 tggtggtgtt ccacagcttg ggtcaggcgc aaattcgctc cattgccaac atccagctca
     2281 aaggcttgcg caaacgcttg gaaggcttgg acttgcattt gcaaattgac gatgcggctt
     2341 tggatgtgtt ggcggaagtg ggcttcgatc ctgtgtacgg tgcgcgccca ttgaaacgtg
     2401 ccatccaagc gcaaatcgaa aacccattgg cgcaaaaact gttggcgggc gtgtacgcac
     2461 cagagtccac catcatgatt accgccaaag acggaca
//
LOCUS       DAOMVK010000342         2496 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_12440_length_2496_cov_2.816059, whole genome shotgun sequence.
ACCESSION   DAOMVK010000342 DAOMVK010000000
VERSION     DAOMVK010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12440_length_2496_cov_2.816059"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(246..1040)
                     /locus_tag="PK856_09945"
     CDS             complement(246..1040)
                     /locus_tag="PK856_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02729.1"
                     /translation="MHRRHFIHSLLAASVLTQTGCITAALMSRALSDGHRMGGNNTGP
                     ATSGRRVHDMGRFQLYADTSLSQLIIVGENQHWILNTGAERDNLKQVLPSIIKSPAAW
                     LFSNASLAMPANMAQGEVPKVFLRFFSVYRPHALSPAQQQTLQALAYETDTNVAKDLA
                     FMNQLVKHGALGANEPTLNRILFYSGKAYQASSAPAGFFEQAIAAGSFPEITETIALN
                     PATQTPRVAFALPASPVRIAASGQVSLNGKPLLPLASRTLLYPYRG"
     gene            complement(1262..2158)
                     /locus_tag="PK856_09950"
     CDS             complement(1262..2158)
                     /locus_tag="PK856_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRL02730.1"
                     /translation="MFDVDVRLLQLFHAIFRNGSITKAAASLNIGQPAASIGLNKLRS
                     HFSDQLFVRVGNHMEATDIAKELAPMVMEALEHIRSVNEYRKAFNPLESNQEFTLSMT
                     DISHLNLLPTLLQYLRVHAPSVRLNVVPIGGQTAGMMSSGDIDLAIGFIPQLEAGFYQ
                     QTLFKQQYVALIRADHPRIQGQELSLDVYLQELHVDVIANGTGHYIIETEMHKQGIAR
                     EVVIKLPSYLGIGLVIQETELIATVPERLSNLLLSRGNLKILDLPFQLPTYSVKQHWH
                     ERMHGNPANQWLRQTCFQLFHF"
BASE COUNT          569 a          557 c          709 g          661 t
ORIGIN      
        1 cctttatggc ggcgaattgg cctgcgattg cggtggcgga ttttgcttgc aaaattttca
       61 tcggcgcgtg tttgtttttg cctttgtatg gggtgttatt gcaattttta atgcgcaaac
      121 tcacccagtt ggcccctgtg caaagtcgcg tgggcaacta ctgattttga tgggttaatg
      181 cttttacagg ccgcttggta tcgttccaag cggcctgtaa gtttgtgggc cattggtgca
      241 aaggtttagc cgcgataagg atagagtagt gtgcgtgaag ccaaaggcag taagggtttg
      301 ccattaaggc tgacttggcc tgaggcggca atgcgcacag gtgaagcggg cagggcaaag
      361 gccactcgtg gggtttgggt ggcagggttc aaggctatgg tttcggtgat ttctggaaaa
      421 gagcccgctg cgatggcttg ctcaaaaaag cccgcaggtg cggacgaggc ttggtaagcc
      481 ttgcctgaat agaaaagtat gcggttgaga gtgggttcgt ttgcgcccaa ggcgccatgt
      541 ttgaccaatt ggttcatgaa ggccaggtct ttggcgacgt tggtgtctgt ttcgtaggcc
      601 aaggcttgca aggtttgttg ttgggctggg ctcaatgcat gcggcctgta aacgctgaaa
      661 aagcgcaaaa agactttggg tacctcgcct tgagccatgt ttgcaggcat ggccaatgaa
      721 gcattgctga agagccatgc ggcgggagat ttgatgatgc ttggtaatac ttgtttgagg
      781 ttgtcgcgct ctgcgcctgt gttcaaaatc cagtgctgat tttcgcccac aatgatgagt
      841 tggctgaggc tggtatcggc atacaattgg aagcggccca tgtcgtgaac acggcggcct
      901 gatgtggcag ggcctgtgtt gttgccaccc atgcggtggc cgtcactcaa ggcgcggctc
      961 attaaagcgg cggtgatgca gcctgtttgc gttaacacac tggcggccag taggctgtgt
     1021 atgaagtggc gtctgtgcat gtttggcctt tctgtattgt ggtaagccat gggtttaaac
     1081 gcttgtctca atcgtgcatg gctcactgta tctcgtgtgg cttgttactg ggatggttgt
     1141 gcatgcggcc tgtaaatgtt tacaggcctc atttaacatg ggaatcagtc agttccatta
     1201 agactgagtg caatacattg aacatgcagc ctcttgctca cccaagcgac agtaaagctg
     1261 cctaaaagtg aaacaattga aaacaggttt ggcgcaacca ttggttggca ggattgccgt
     1321 gcatgcgctc gtgccaatgc tgtttcacac tgtaagtggg cagctgaaac ggcaaatcca
     1381 atatcttcaa attgccacgt gagagcagca aattgctcaa gcgctcgggc acggtcgcaa
     1441 tcaattcggt ttcttgtatc accaagccta tgcccaaata actgggcagc ttaatcacca
     1501 cttcgcgggc gatgccttgc ttgtgcatct cggtttcaat gatgtaatgg cctgtgccat
     1561 tggcaatcac atccacatgc aattcttgca aatacacatc caaactcaat tcttgtcctt
     1621 gtatgcgcgg gtggtcggcg cggattaagg ccacgtattg ttgcttgaac aaagtctgtt
     1681 ggtaaaagcc cgcttccaat tgcgggataa agcctatggc caaatcaatg tcgccgctgc
     1741 tcatcatgcc cgcggtttgt ccgcctatcg gcaccacatt caaacgcacc gaaggcgcgt
     1801 gcacgcgcaa atactgcagc aaagttggca gcagattcaa atggctgatg tcggtcatgc
     1861 tcaaggtgaa ttcttgattc gattccaagg ggttgaaggc tttgcggtat tcattgaccg
     1921 agcggatgtg ttccaaggcc tccatcacca tcggcgccaa ttctttggcg atgtcggtgg
     1981 cttccatgtg gttgcccacg cgcacaaaca attggtcaga gaaatgactt ctgagcttgt
     2041 tcaagcctat gctcgccgct ggttggccga tattgaggct ggcagcggct ttggtaatgc
     2101 tgccatttcg gaaaatggca tggaaaagtt gcaataatcg aacgtctaca tcaaacatgg
     2161 tggctaaggt tattgatttg attaatagag cgtattgcat ttgggctatg gaaacaaggg
     2221 ttttgagttg ttaaagtgcg ctgcagacac agtgtgatgc aagtcacatt cagtattgca
     2281 ttgcaaaaac agcggcttat tggcgcacac atggagatga gatggaagaa ttggcgactt
     2341 tggcagattt accaaacgaa tatgtcagcg cattggagca aaaaaatttg gtgccattgt
     2401 ggcctagcct gcgcgatgtg atgccgccag aaacgccacg tccgcgcaca caggccactt
     2461 gttggaaatt ccaagacatc cgtccgttgc tcatac
//
LOCUS       DAOMVK010000343         2489 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_12491_length_2489_cov_2.548891, whole genome shotgun sequence.
ACCESSION   DAOMVK010000343 DAOMVK010000000
VERSION     DAOMVK010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12491_length_2489_cov_2.548891"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="PK856_09955"
     CDS             complement(<1..824)
                     /locus_tag="PK856_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HRL02731.1"
                     /translation="MNLPKHVKIVEVGPRDGLQNEKQAIDTATKVELIARLGASGLKT
                     IEATAFVSPKWVPQMADNAEVMRQITRLDGVTYPVLTPNLKGLDAALATGVTEVAVFA
                     AASEAFSQKNINCSISESIERFVPVIESAREHGVAVRGYVSTVVGCPYQGDVPPEKVA
                     EVSKALFDLGCYEISLGDTIGVGTPLKVQTMLAEVSKAVPMDKLAGHFHNTYGMAIAN
                     IYASLQMGMAVFDASVAGLGGCPYAKGASGNVATEDVVYLLNGLGIDSGVDLSHLV"
     gene            complement(836..>2489)
                     /locus_tag="PK856_09960"
     CDS             complement(836..>2489)
                     /locus_tag="PK856_09960"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRL02732.1"
                     /translation="TSKALMEKADVPLTPGYHGNNQDPEFLHQEADKIGYPVLIKASA
                     GGGGKGMSLVENSADFQASLASCKREAKSSFGNDDVLIEKYIVRPRHVEVQVFGDTHG
                     NYVHLFERDCSVQRRHQKVLEEAPAPNVPEHYLTQMREAAINAARAVGYVGAGTVEFI
                     AQQSGEFYFMEMNTRLQVEHPVTEMITGQDLVEWQLRVASGEPLPKQQAELSVHGHAM
                     EARIYAEDPDKGFLPSIGKITYMHQPQTSEVVRVDSGVVEGDTISAYYDPMIAKLIVW
                     GEDRATALQRMYDALGEFNVLGLGNNVGFLRRLASNPAFSNIELDTGLIEKQHDLLFP
                     EPAPVADEIVLMAALALSLEGKQASQQHSGNSVEPQSPWYKTDGWRMNAAGRQILNFK
                     HAGQDYSVAVEFAGDESYKLVYQGQEHVAKAAVLLTGRLRMAIDGYQTQVRHVFANNQ
                     LALFVNGAAYTFEYINPYVADAGSNEVGGQLRAPMPGSVVQVMVEAGQAVKKGQALLI
                     LEAMKMEHTITAPNDGVVKQVLFAVGDQVGDGDELLQLEADE"
BASE COUNT          526 a          733 c          606 g          624 t
ORIGIN      
        1 tccaccaagt ggctcaaatc cacgccgctg tctatgccca agccattgag caggtaaacc
       61 acatcttcgg tggccacatt gccgctcgcg cctttggcat atgggcaacc gcccaagcct
      121 gccaccgaag catcaaacac cgccatgccc atttgcaatg aggcgtaaat attggcgatg
      181 gccatgccat aggtgttgtg gaaatggcct gccaatttgt ccatcggtac ggctttgctc
      241 acctcggcca acatggtttg cactttcaat ggcgtgccca cgcctatggt gtcgcccaaa
      301 gaaatctcat aacagcccaa atcaaacaag gctttggaca cttcggccac tttttcaggc
      361 ggcacatcgc cttggtaagg acagcccacc acggtagaca catagccgcg caccgccacg
      421 ccatgctcgc gcgcgctttc aatcacaggc acaaagcgct ctatcgattc ggagatgctg
      481 caattgatgt ttttttgcga gaaggcttcc gaagccgcgg caaacaccgc cacctcggtc
      541 acacctgttg ccaaggcagc gtccaagcct ttcaaattgg gcgtgagcac gggataagtc
      601 accccgtcca aacgggtgat ttggcgcatc acttcggcat tgtcggccat ttgtggcacc
      661 cattttggcg acacaaacgc ggttgcttct atggttttca ggccgcttgc acccaagcgt
      721 gcaatcaatt ccactttggt ggccgtgtct atcgcttgct tttcgttttg caagccatcg
      781 cgtgggccta cttcaacgat tttgacgtgt ttaggcaaat tcatcgcgct tcctttcact
      841 cgtctgcttc caattgcaac aactcatcgc cgtcgcccac ttggtcgccc accgcgaaca
      901 acacctgttt caccacgcca tcgtttggtg cggtaatggt gtgttccatt ttcatcgctt
      961 ccaatatcag caaggcttgg ccttttttca cggcctgacc cgcttccacc atcacttgca
     1021 ccaccgagcc tggcatgggc gcgcgcaatt gtccgcccac ttcgttgctg cccgcatcgg
     1081 ccacatacgg attgatgtat tcaaacgtgt aagccgcgcc gttcacaaac aaagccaatt
     1141 ggttgttggc aaacacatgg cgcacttggg tttggtagcc atctatcgcc atgcgcaagc
     1201 ggcctgtaag caatacggcg gctttggcca cgtgttcttg gccttggtaa accagtttgt
     1261 agctttcatc gcccgcgaat tccaccgcca cgctgtagtc ttgccctgcg tgtttgaaat
     1321 tcaaaatctg tctgcccgcc gcattcatgc gccagccatc ggttttgtac cacggtgatt
     1381 gtggctcgac actgttgccg ctgtgctgtt ggctggcttg cttgccttcc aaagacaagg
     1441 ccaaagccgc catcaacacg atttcatcgg ccacaggcgc aggttctgga aacagcaaat
     1501 cgtgttgttt ctcgattaaa cccgtgtcca attcaatgtt ggaaaacgcg ggattgctgg
     1561 ccaaacggcg caaaaagccg acgttattgc ccaaacccaa gacattgaat tcgcccaaag
     1621 cgtcgtacat gcgttgcaaa gcggtggcac ggtcttcgcc ccacacgatt aatttggcaa
     1681 tcatggggtc gtaataggcg ctgatggtgt cgccttccac cacgccactg tccacacgca
     1741 ccacctcaga tgtttgtggc tgatgcatgt aagtgatttt gcctatggat ggcaaaaagc
     1801 ctttgtctgg gtcttcggcg taaatgcgcg cttccatcgc atggccgtgc acagacaatt
     1861 ctgcttgttg cttaggcaaa ggctcgcccg aagccacgcg caattgccat tccaccaagt
     1921 cttgccctgt aatcatctcg gtaacagggt gttccacttg caaacgagtg ttcatttcca
     1981 tgaagtagaa ttcgccgctt tgttgggcaa tgaattccac cgtgcccgca cccacataac
     2041 caacggcacg cgctgcattg atggcggctt cgcgcatttg ggtgaggtag tgttcaggca
     2101 cgttcggcgc tggcgcttct tccaacactt tttggtggcg gcgttgtacc gagcaatcgc
     2161 gttcaaacaa atgcacgtaa ttgccgtggg tgtcgccaaa cacctgtact tccacatggc
     2221 gtgggcgcac gatgtatttt tctatcaaca cgtcatcgtt gccaaagctg gatttggctt
     2281 cgcgtttaca cgacgccaat gaggcctgaa aatcggcact gttttccacc aaactcatgc
     2341 ctttgccgcc accgcctgca ctggctttaa tcaacacggg atagcctatc ttatcggcct
     2401 cttggtgcaa aaattctggg tcttggttgt tgccatggta gccaggcgtg agcggcacat
     2461 cggctttttc catcaaagct ttagacgtg
//
LOCUS       DAOMVK010000344         2473 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_12595_length_2473_cov_2.595533, whole genome shotgun sequence.
ACCESSION   DAOMVK010000344 DAOMVK010000000
VERSION     DAOMVK010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12595_length_2473_cov_2.595533"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PK856_09965"
     CDS             complement(<1..662)
                     /locus_tag="PK856_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009427360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HRL02733.1"
                     /translation="MTVIKQQDFIQSIADAFQFISYYHPKDFIQALTKAWEKEENPAA
                     KDAMTQILVNSRMCAEGRRPICQDTGIAVVFLKVGMNVQWDAELSVQDMVNEGVRQAY
                     AHPDNKLRASILRDPAGKRQNTKDNTPAVVHMEIVKGDKIEVICAAKGGGSENKSKLA
                     MLNPSDSIVDWVLKTVPTMGAGWCPPGILGIGIGGTPEKAALMAKESLMGHIDIHELQ
                     DKA"
     gene            854..2275
                     /gene="trkA"
                     /locus_tag="PK856_09970"
     CDS             854..2275
                     /gene="trkA"
                     /locus_tag="PK856_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HRL02734.1"
                     /translation="MKILILGCGQVGSAVAKSLAQQPNNDVTVIDINAAAIQRLSDSL
                     DIQTLIGSCTSPYILQAAHIEDCDLLLALTGHDETNLVACQLASRLYNTPESIARVRQ
                     AELVNAIANANEDEDEGEINQNIFDVSYYIRPEQLVTEQLFGLFQYHQVLQVLSFDDD
                     SARLTVVQAQAGGAMIGKKLEQMQDVLTERGKGKYDCQVSAIYRNNRLIIPQQETAII
                     EGDEIYVLARTEHMTQVLRLFRPDEDLGRRVMIAGGGNIGYRLAKLLEPSCNVKIIEH
                     GQARAEWLADNLNNTLVLQGSATDESLLTQENIDEIDVFCALTNDDEDNVMSSLLANN
                     LGAKQIMTIINRTSYVDLLQGNQIDIVVSPHLITIGSILSHIRRGDMVAVYPLRHGTA
                     EAIEVILHGDRKTSKLVGRRLQEIKLPNSCYFAAVGRKGEWVMAHKRDWILQEGDHVI
                     FFVAQSRYVQELEKTIAVKFGFF"
     gene            2293..>2473
                     /locus_tag="PK856_09975"
     CDS             2293..>2473
                     /locus_tag="PK856_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HRL02735.1"
                     /translation="MYKLLPIVHILAKLGILFAALLMLPALISWIFGDGLAMYLTSIS
                     MVTMFIAFVVWVLTAK"
BASE COUNT          598 a          588 c          604 g          683 t
ORIGIN      
        1 gctttgtctt gcaattcgtg gatgtcgatg tggcccatca aggattcttt ggccatcaaa
       61 gcggcttttt caggtgtgcc accgatgcct atgcccaaga tgccaggagg acaccaacct
      121 gcgcccatgg taggcacggt tttcaatacc caatccacaa tggaatcgga tgggttgagc
      181 atcgccaatt tcgatttgtt ttcagagccg ccgcctttgg ccgcacaaat cacttcgatt
      241 ttgtcgcctt taacgatttc catgtgcacc acggctggcg tgttgtcttt ggtgttttgg
      301 cgtttgcccg ctgggtcgcg caagatggag gcacgcaatt tgttgtctgg atgggcatag
      361 gcttgacgca cgccttcgtt caccatgtct tgcacagaaa gctcagcatc ccattgcaca
      421 ttcatgccga ctttcaaaaa taccacagca atgcctgtgt cttggcaaat cggacgacgg
      481 ccttcggcac acatgcgact gttcaccaaa atctgcgtca tcgcgtcttt ggcagcaggg
      541 ttttcttctt tttcccacgc tttggttaat gcttgaatga agtctttggg gtggtaataa
      601 ctgatgaatt gaaatgcatc agcaatgctt tggataaaat cctgttgctt gatcacagtc
      661 atggtgaacc ttttttagta tgaggtgaat gttccaggca atcattttag ccttttcaca
      721 catattcgtc acccacactt gctggtcact atcgcattta tggtggtttt gaaatcattc
      781 atttcgcaca gctgagcagg aattttaaat tttcgttaaa atgcctgaac tttgtgtttg
      841 ttcaggttgt tctatgaaga ttttgatttt gggctgcggc caagtgggct cagcagtggc
      901 caagagtttg gcgcaacagc ccaataatga tgtgacagtc atcgacatca atgccgctgc
      961 catccaacgc ttgagtgaca gcttggacat acaaaccctc atcggatctt gtacctcacc
     1021 ttatatttta caggccgctc acattgaaga ttgtgacttg cttttggcgc ttacgggtca
     1081 tgatgaaacc aatcttgtag cctgccaact ggcctcgcgt ttgtacaaca cgccagaatc
     1141 cattgcccgc gttcgccaag cagaattggt aaacgccatt gccaatgcca acgaagatga
     1201 agacgaaggc gaaatcaacc agaacatttt tgatgtgtcg tattacattc ggcctgagca
     1261 attggtgacc gagcaattat ttggcttgtt ccaataccat caggtattgc aagtcttgtc
     1321 atttgacgat gactcggccc gcctcactgt cgtgcaggcg caggcaggcg gcgccatgat
     1381 aggcaaaaag ttggaacaaa tgcaggatgt tttaacagag cgtggcaaag gcaaatatga
     1441 ctgccaagtc agcgccattt atcgcaataa tcgcttaatc attccacaac aagaaaccgc
     1501 cattattgag ggcgatgaaa tctatgtgtt ggcacgtacc gaacacatga cacaagtctt
     1561 gcgcttgttc cgccctgatg aggatttagg acggcgggtg atgattgcgg gcggtggtaa
     1621 cataggctac cgcttggcca agctcttaga accgtcctgt aatgtgaaaa tcattgagca
     1681 cggccaagcc cgcgccgaat ggttggctga taacctgaac aatactttgg tcttgcaagg
     1741 ctcagccacc gatgaatctt tgttgacaca agaaaacatt gatgaaatcg atgtgttctg
     1801 cgccctcacc aacgatgacg aagacaatgt gatgtctagc ctgttggcca ataatttggg
     1861 cgccaaacaa atcatgacca tcatcaaccg cacgtcttat gtggacttgt tacaaggcaa
     1921 tcaaattgac attgtggtgt cgccacatct gattaccata ggctcgattt tgtcgcacat
     1981 ccgccgcggc gacatggtgg cagtgtaccc gctgcgccat ggcacggctg aagccatcga
     2041 agtgattttg cacggtgacc gcaaaacctc gaaactggtt ggccgccgcc tacaggaaat
     2101 caagttgccc aacagctgtt attttgccgc agtgggccgc aaaggcgaat gggtgatggc
     2161 gcacaaacgc gattggattt tgcaagaagg cgaccatgtg atcttctttg tggcccaaag
     2221 tcgctacgtg caagaattgg aaaaaaccat tgcggttaaa tttggcttct tttaatcgag
     2281 ggcagcactt ttatgtataa acttctgccc attgtgcaca ttttggccaa gctcggcatt
     2341 ttgtttgcgg ccttgttgat gttgcctgca ttaatatcgt ggatatttgg cgatggcttg
     2401 gccatgtatt tgaccagtat ttccatggtc accatgttca tcgcgtttgt ggtgtgggta
     2461 ttaacggcca aat
//
LOCUS       DAOMVK010000345         2466 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_12641_length_2466_cov_3.574036, whole genome shotgun sequence.
ACCESSION   DAOMVK010000345 DAOMVK010000000
VERSION     DAOMVK010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12641_length_2466_cov_3.574036"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="PK856_09980"
     CDS             complement(<1..455)
                     /locus_tag="PK856_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HRL02736.1"
                     /translation="MALSHIWVAFFLITFVVACIKLLGGDTEIFEKIVKAMFDSAATG
                     ATISLGLIGMMTFALGIMKIGEKGGMIAIIARLVGPFFNKLFPEIPKNHPALGSILMN
                     FSANALGLDNAATPLGLKAMKELQELNPVKDTASNAQIMFIVLNASSLT"
     gene            complement(561..1214)
                     /locus_tag="PK856_09985"
     CDS             complement(561..1214)
                     /locus_tag="PK856_09985"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HRL02737.1"
                     /translation="MTLPAWIETISLQNELVQILVSLLIGLLIGGEREYHNKSAGLRT
                     FILISFGACLCTILSIKIGGSSPDRIAANIITGIGFLGAGAIFKDENKVSGITTASTI
                     WATASLGMCVGSGHTALAIFGAVLVWFVLYVMASLQNWIDQYNKIIDYKLTLPTINDV
                     SYCEDLFHQYGLKPTLLREAKSPQQCVLTWRLAGRYSQHQAFLAKLRQDAVIQNYQY"
     gene            complement(1261..2334)
                     /gene="hisC"
                     /locus_tag="PK856_09990"
     CDS             complement(1261..2334)
                     /gene="hisC"
                     /locus_tag="PK856_09990"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRL02738.1"
                     /translation="MESVFWSDGVAGLHPYVPGEQPQIANLVKLNTNENPFPPSEQVL
                     AAIATAAQSGLQRYPDPQSADLLQALASYHGLENGNVFVGNGSDEVLAHAFRAFYVKQ
                     KPLLMPDISYSFYGVYAALFGIQCQTVALNVDLVVDVNDYLAIAADSVAGVVIANPNA
                     PTGVAISLADIERVLQHFANVAVLIDEAYVDFGAESACKLIAKYPNLLVVQTLSKSRS
                     LAGLRIGFALGSAELIDGLVRVKDSFNSYPLDKIAQAAAVAAFADQAYFEQCRDAVVS
                     EREIVTAALQARSFTVLPSATNFVFASHAGYKGRDLLAALRERAVLVRQFEKPRIADF
                     LRISIGTAAQNQALIAALDDILA"
BASE COUNT          670 a          632 c          582 g          582 t
ORIGIN      
        1 aaggtgaggc tggacgcatt gagcacaatg aacatgattt gcgcattgct ggccgtgtct
       61 ttcacaggat tgagctcttg taattccttc atcgccttca agcccaatgg tgtggcggca
      121 ttgtccaaac ccaatgcatt ggcggagaaa ttcatcaaga tagaacccaa agcgggatgg
      181 tttttgggga tttcgggaaa gagtttgttg aaaaatggcc ccaccaaacg ggcaatgatg
      241 gcaatcatgc cgcctttttc accgattttc atgatgccca aggcaaacgt catcatgcct
      301 atcagcccca aggaaatggt ggcacctgtg gcggcactat caaacatggc tttgacgatt
      361 ttttcaaaaa tctcggtgtc accacccagc agcttgatgc aagccaccac aaaagtaatg
      421 aggaaaaacg ctacccaaat gtgactcaaa gccatggcaa tcgtccgaat tcaaacaaaa
      481 tgccattcta gcccaagccc attggaacat gcactcaaaa ccttgccaca caagacttac
      541 aggccgctta atctttgatt ttaatattga tagttttgaa tcaccgcgtc ttggcgcaac
      601 ttcgccaaaa aagcctgatg ctgactgtaa cgccctgcca aacgccatgt gagcacgcat
      661 tgctgtggtg atttggcttc gcgcaacaag gtgggcttta agccgtattg gtgaaacaag
      721 tcttcacaat agctgacatc attgatggta ggcaaagtca gtttgtaatc aatgattttg
      781 ttgtattggt ctatccagtt ttgcagcgag gccatcacat acaacacaaa ccacaccaat
      841 accgccccaa aaatcgccaa ggcagtgtgg cccgagccca cgcacatgcc taaagaagct
      901 gtggcccaaa tggtactggc ggtggtgatg ccgctgactt tgttttcgtc tttgaatatg
      961 gcacccgcgc ccaaaaagcc tatgccggtg atgatgttgg cggcgatgcg gtcgggactg
     1021 ctgccgccaa ttttgatgga caaaatggta cacagacacg cgccaaagct gatgaggata
     1081 aacgtgcgca ggccagccga tttgttgtgg tattcgcgct cgccaccaat gagcaaaccg
     1141 atgagcagcg acaccaaaat ctgcaccagt tcgttttgca aagagatggt ttctatccat
     1201 gctggcaatg tcatcgtgct caccttaggt ttacaggccg cttatggcgc gctatggctg
     1261 ttaggccaag atgtcatcta aagcggcaat caaggcttgg ttttgcgcgg ccgtgccgat
     1321 gctgatgcgt aagaagtcgg caatgcgcgg tttttcaaat tggcgcacca acactgcccg
     1381 ctcacgcaaa gccgccaaca aatcacggcc tttataccct gcatggctgg caaacacaaa
     1441 attggtggcc gatggcaata ccgtaaaact gcgcgcttgc aaggccgctg tcacgatttc
     1501 tcgctcagac accaccgcat cgcggcactg ttcaaaatag gcttggtcgg caaaggcggc
     1561 cacagcagcg gcttgggcga ttttgtccag cggataagaa ttgaagctgt ccttcacccg
     1621 caccaaacca tcgattaact cagccgaacc caaagcaaaa ccgatgcgta aacctgccaa
     1681 tgaacgtgat ttggacagag tttgcaccac caacaaattt ggatacttcg caatcagttt
     1741 acaggccgat tctgcgccga aatccacata ggcttcgtct atcaacaccg ccacattggc
     1801 aaagtgttgc aacacacgtt caatgtcggc aaggctgatg gccacaccag tgggtgcatt
     1861 tggattggcg atgaccaccc ctgcaacgct gtctgcagct atggccaaat aatcgttcac
     1921 gtccaccacc aaatcgacgt tcaaagccac ggtttggcac tgaataccaa acaaagccgc
     1981 gtacacgccg taaaaactgt aggaaatatc gggcatgagc aacggttttt gtttcacata
     2041 aaaagcacga aacgcatggg ccaatacttc atctgagcca ttgcccacaa acacattgcc
     2101 gttttccaag ccatggtaag acgctaaggc ttgcaataaa tcagccgatt gtggatcagg
     2161 atatcgctgt aagccgcttt gggctgcagt ggcaatcgct gccaacactt gttcagatgg
     2221 ggggaatggg ttttcattgg tgttgagttt caccaaattg gcaatttgcg gctgctcacc
     2281 tggtacgtat gggtgtaagc ctgccacacc atcactccaa aatacagatt ccacgtgttt
     2341 tcctttgatt tcgatgttgc ttcactaaaa tgcttaatgt agtgagtttt cacggagtac
     2401 acaagcatga caacacgatg caatttcaaa catgtcagtg ctgttaaaac ataaaaaaag
     2461 cgtcct
//
LOCUS       DAOMVK010000346         2458 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_12702_length_2458_cov_2.517686, whole genome shotgun sequence.
ACCESSION   DAOMVK010000346 DAOMVK010000000
VERSION     DAOMVK010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12702_length_2458_cov_2.517686"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..>371
                     /locus_tag="PK856_09995"
     CDS             21..>371
                     /locus_tag="PK856_09995"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRL02739.1"
                     /translation="MGSLGDFNTLNPYLLKGDKLPGLAELGDNFVFDTLMVESWDEPF
                     AMYGLLASDIALAADKMSVTFTLNPQARFQNGDAVLAKDVVYTFNTITKDEAATPLYR
                     FYYADVKRAVALDEH"
     assembly_gap    372..381
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <382..1657
                     /locus_tag="PK856_10000"
     CDS             <382..1657
                     /locus_tag="PK856_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRL02740.1"
                     /translation="TKPNAELHLILGQIQIFSHKSFPLGFAKSANVLPIGSGPYRLAR
                     HEFGQLVQYQRRPDYWAQTLPIRKGFWNFDQIRFKVYRDPTAFTEALKAGKIDLTYEN
                     SAKNWARAYANPSLAERGLQMQSFAHKNNAGLQGYALNTRREPLNDVKFRQALGLSFD
                     FETINQQIFYGLYKRPYSYFTNSELAAEGLPTQAELAVLEPLRKQLPARVFEQTVPMP
                     AKVSPQLGVRPNLLQARTLLLQAGYRYRDGHLIDKQGRPVSLEYLTNAKSHERAAGKW
                     QRDLAKLGIELKIRLVDSAIYQKRIQAFDYDIITHVYGQSQSPGNEQMEMHSCASAKT
                     PPSRSGNMVGSCEPAIDALLKRFEHFESREELVAVSKSLDRVLRHQYYVIPQWYSDEW
                     RMVYWNKFEQPKTLPLYYGPTTWALMTWWQKQ"
     gene            1785..>2458
                     /gene="cysK"
                     /locus_tag="PK856_10005"
     CDS             1785..>2458
                     /gene="cysK"
                     /locus_tag="PK856_10005"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HRL02741.1"
                     /translation="MNIANNITELVGNTPLVKLNRVTDGAEATVLAKLEFFNPGSSVK
                     DRIAVAMIDAAEKTGKINKNTTIVEPTSGNTGIGLAMVCAARGYKLIITMPETMSRER
                     RMLMRAYGAELVLTPGPEGMGGAIAKAKELAAANPDSFFIPQQFENPANPEVHRNTTA
                     LEIWNDTDGKVDIVVAGVGTGGTITGIGEVLKAKNPNIQIIAVEPAASPVLSGGPKGP
                     HPIQGIG"
BASE COUNT          676 a          553 c          612 g          607 t
ORIGIN      
        1 caaaaggcgg cagcttggtc atgggatctt tgggggattt caataccctg aatccgtact
       61 tactcaaagg ggacaagttg ccagggttgg cagagcttgg ggacaacttt gtgtttgata
      121 ctttgatggt ggaaagttgg gatgagcctt ttgccatgta cggattattg gccagcgaca
      181 ttgcattggc tgccgataaa atgtcggtca cattcacact gaatccacag gctcgctttc
      241 aaaatggtga tgctgtcctt gccaaagatg tggtttatac ctttaatacc attaccaaag
      301 atgaagcggc aacgccgctg tatcgattct attatgccga tgtgaagcga gcggtggcct
      361 tggatgagca cnnnnnnnnn ntaccaagcc aaatgcagaa ctgcatctga tattggggca
      421 gatccagatt ttttcacaca aaagtttccc tttgggtttt gccaagtctg ccaatgtgtt
      481 gcccataggc tcggggccgt atcgcttggc caggcatgag tttggacaat tggtgcagta
      541 tcaacgtcgt cctgattatt gggcgcagac tctgcccata cgcaaaggct tttggaattt
      601 tgatcagatt cgctttaagg tttaccgcga cccaaccgct ttcacggaag ccctaaaagc
      661 agggaaaatc gatttaacct atgagaattc ggccaaaaac tgggcaagag cctatgccaa
      721 tccaagtttg gcagagcgtg gcttgcagat gcaatctttt gctcacaaga ataatgcggg
      781 tttacaaggt tatgccctga acacgcgccg tgagcctttg aatgatgtca aattcagaca
      841 ggctttgggt ttgagctttg attttgaaac cattaatcag cagatttttt atggtttgta
      901 taaacggcct tacagctact tcaccaacag cgagttagca gctgaaggtc tgccgacaca
      961 agctgaattg gccgtattgg agcccttgcg caagcaattg cctgcgcgtg tgtttgaaca
     1021 aacagtgcca atgcctgcca aagtttcgcc gcaactgggc gtaagaccta atttattaca
     1081 agcacgtaca ttgttgttac aggctggcta tcggtatcga gatgggcacc tgatagacaa
     1141 gcaagggcgg cctgtaagtc tggaatacct caccaatgcc aaaagccatg aacgggccgc
     1201 aggcaaatgg caacgcgatt tggccaagct gggcatagag ttgaaaattc gtttggtcga
     1261 cagtgccatt taccagaaac gcatccaagc gtttgattac gacatcatta cccatgtgta
     1321 tggccaaagc caaagccctg gcaatgagca gatggaaatg cacagctgcg cctctgcaaa
     1381 aaccccacct tcacgcagtg gcaatatggt tggcagctgt gagccagcca tagatgcctt
     1441 attgaaacgc tttgaacatt ttgagtctcg tgaagaattg gtggcagttt ccaaatcctt
     1501 ggatagggtg ttaagacacc aatactatgt gattccacag tggtacagcg atgaatggcg
     1561 catggtgtac tggaacaagt ttgaacaacc caagactttg cccttgtatt acggcccaac
     1621 gacatgggca ttgatgacat ggtggcagaa gcagtgacgt aaagtgtacc aaactgctat
     1681 atggttatag ttttatgttc tttgccgcag gcaaggttag tgcgtcataa tacccgaaca
     1741 gaattttaat ccacttgaac aaaatacaat aaggacgata atcaatgaac attgcaaaca
     1801 atatcactga attggttggc aataccccac tggtgaaatt aaaccgagtg acagacggtg
     1861 ctgaggcaac cgttttggcc aaattggaat tcttcaatcc aggttcaagt gtgaaagacc
     1921 gcattgctgt tgccatgatt gatgctgctg aaaaaactgg aaaaatcaat aaaaacacca
     1981 ccattgttga gccaacatca ggcaataccg gcattggttt ggccatggtg tgtgccgctc
     2041 gcggttacaa attgatcatt accatgcctg aaaccatgag ccgtgagcgc cgcatgttga
     2101 tgcgtgctta tggtgctgag ctggttttga ccccagggcc tgaaggcatg ggcggagcca
     2161 ttgccaaagc caaagaattg gcggcggcca atcctgattc tttcttcatt ccacaacaat
     2221 ttgaaaatcc agccaaccca gaagtgcacc gcaacaccac cgcattggaa atttggaacg
     2281 ataccgatgg taaagtagac atcgttgtag caggcgtggg tacaggcggc acgatcacgg
     2341 gcataggcga agtgttgaaa gccaaaaacc caaatattca aatcatcgca gttgagccag
     2401 cagcatcgcc tgtattgtca ggaggcccta aaggcccaca tccgattcaa ggcatagg
//
LOCUS       DAOMVK010000347         2457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_12713_length_2457_cov_3.554122, whole genome shotgun sequence.
ACCESSION   DAOMVK010000347 DAOMVK010000000
VERSION     DAOMVK010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12713_length_2457_cov_3.554122"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PK856_10010"
     CDS             <1..207
                     /locus_tag="PK856_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003743295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRL02742.1"
                     /translation="NDILPAVPNPAYVNTDRAQAIQYAVQNAAANDIILIAGKGHETY
                     QDIAGHKNHFSDFECARAALAERH"
     gene            223..1587
                     /gene="murF"
                     /locus_tag="PK856_10015"
     CDS             223..1587
                     /gene="murF"
                     /locus_tag="PK856_10015"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRL02743.1"
                     /translation="MSELTLAFVQVALSAPVGAQADVATRIITDSRQAGVGDVFVALV
                     GEQFDGHDYVADVLAKGVAAVIVTREVVAGDARQIVVADTLAALGQVAQAWRLKWNPK
                     IVALTGSNGKTTVKEMCAVILRTALGESAVLATAGNFNNEIGMPLTLLRLQAAHKVAV
                     LEMGMNHFGELARLTQIAQPDVALVNNASRAHIGNDFNGVTDIARAKSEIYAGLNTET
                     GMAILPCEDANVAIFEAAAQGYKQTTFGLHVGDVHANKIVVSPLFSDFVLTDGTEQIE
                     VHLPVAGEHNVYNAAAAAAAMRAIGIEWSDIAAGLAQFHNVKGRLQGFTSKNGALIVD
                     DTYNANPDSMKAALDVLSRFDGTKVFVMGAIGELGATSLQLHAEVGTYANEKDLTALL
                     ALGEDAKAASKACVVGQYFENVDALLAALRPYDVAGSTILVKGSRFMQMERVVEALLH
                     TTGK"
     gene            1597..>2457
                     /gene="mraY"
                     /locus_tag="PK856_10020"
     CDS             1597..>2457
                     /gene="mraY"
                     /locus_tag="PK856_10020"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRL02744.1"
                     /translation="MLLWLADLLGNWFTGFNLFQYTTFRAVMAALTALLFGLLFGPWT
                     IRKLTELKVGQAVRRDGPQTHLVKTGTPTMGGTLIITAIIVSVLLWGNLANKYVWILL
                     GVTVGTGALGFYDDWRKVVYKDPNGVSGRFKIVWQSVVALAAGLFLLYNTNLTSTEQI
                     IIPYLKNVSIAYPLGAVGFLVLTYLVIVGTSNAVNLTDGLDGLAAFPIVLVSGGLAIF
                     AYASGHAEFAKYLQLPHIPGANEVMIFCAAICGACLAFLWFNAYPAQVFMGDVGALAM
                     GAALGTVAVIV"
BASE COUNT          550 a          530 c          701 g          676 t
ORIGIN      
        1 aatgacattt tgccagccgt gcctaaccct gcctatgtga ataccgaccg tgcgcaagcc
       61 atacagtatg cggtgcaaaa cgcagcggcc aacgacatca ttttaatcgc tggcaaaggt
      121 cacgaaacgt atcaagacat tgctggccac aaaaaccatt tttccgattt tgaatgtgcg
      181 cgcgctgctt tggcagagcg tcactgattg aagacacaag atatgagtga attgactctg
      241 gcatttgtac aggtcgcttt aagtgcccct gtgggcgcac aagcagatgt ggccacacgc
      301 atcatcaccg acagccgcca agctggtgtg ggggatgtgt ttgtggcctt ggtaggtgaa
      361 caatttgatg gccacgatta tgttgccgat gtgttggcca aaggcgtggc ggcagtgata
      421 gtgacccgtg aggtggtggc tggcgatgcg cgccaaatcg tggttgccga cactttggct
      481 gctttgggac aggttgctca agcatggcgc ttgaaatgga atccaaaaat cgtggcttta
      541 accggctcga atggcaaaac cacggtcaaa gaaatgtgtg cggtaatatt gcgcactgct
      601 ttgggtgaga gcgctgtgtt ggccactgct ggcaatttca acaatgaaat cggcatgcct
      661 ttaaccttat tgcgtttaca ggccgctcac aaagtggcgg ttttggaaat gggcatgaac
      721 cattttggtg aattggcgcg tttaacccaa atcgctcaac ctgatgtggc cttggtgaac
      781 aatgcatcgc gtgcgcacat cggcaatgat tttaatggcg tgaccgacat tgctcgcgcc
      841 aaaagcgaga tttatgcagg tttgaatact gaaactggca tggcgatttt gccatgtgaa
      901 gacgcaaatg tggcgatttt cgaggcggct gcccaaggct acaaacagac cacttttgga
      961 ctgcatgtgg gtgatgttca tgcaaacaag attgttgtgt caccattgtt cagtgacttt
     1021 gtgttgaccg atggcacgga gcaaattgaa gtgcatctgc ctgtggcggg tgagcacaat
     1081 gtctataacg ccgctgccgc tgccgctgcc atgcgtgcca taggcataga gtggtcagac
     1141 atcgctgcag gcttggccca atttcacaat gtcaaaggcc gtttacaagg ctttacatcg
     1201 aaaaatggcg cattgattgt tgatgacact tacaatgcga accccgactc gatgaaagcg
     1261 gctttggatg ttttaagccg ttttgatggc acaaaagtgt tcgtaatggg tgcaattggt
     1321 gaattaggtg caacatcgtt gcaattgcat gcagaagtcg gcacttatgc caatgaaaag
     1381 gatttaacgg ctttgttggc tttgggcgaa gatgccaaag cggccagtaa agcttgtgtg
     1441 gttgggcaat attttgagaa tgtggatgcg ctgcttgcgg ctttgcgtcc ctatgatgtg
     1501 gcgggctcga ccattttggt caaaggttcg cgttttatgc aaatggagcg tgtggtggaa
     1561 gccttacttc acaccactgg taaataggac aatgttatgt tgttgtggtt agccgattta
     1621 ttaggcaatt ggttcacggg ttttaactta tttcagtaca ccacattccg agcagtgatg
     1681 gcggcgctta ccgccttgct gttcggtttg ttgtttggcc cttggaccat acgcaaattg
     1741 accgaattga aagtgggcca agcggtgcgc cgtgatggcc cgcagaccca tttggtgaaa
     1801 acaggtaccc cgaccatggg cggtacttta atcatcaccg ccatcattgt tagcgtgttg
     1861 ttgtggggca atttggccaa taaatatgtg tggatattgt tgggcgtgac tgtgggtacg
     1921 ggcgctttgg gtttttacga tgactggcgc aaagtggtgt acaaagaccc gaacggcgtt
     1981 tcaggccgct ttaaaatagt gtggcaatcg gtggtggctt tggcagcagg cttgttcttg
     2041 ctttacaaca ccaatttaac gagcaccgaa caaatcatca ttccctattt gaaaaatgtt
     2101 tccattgcct acccgttggg cgcagtgggc tttttggttt tgacttactt ggtgatagtg
     2161 ggtacttcca acgcggtgaa cttgaccgat ggcttggacg gtttggcggc cttcccaatc
     2221 gtgttggtgt ctggtggctt ggccattttt gcctatgcca gtggtcatgc tgaatttgcc
     2281 aaatacctgc aattgcccca catccctggt gcaaacgagg taatgatatt ctgtgcggcc
     2341 atttgtggcg catgtttggc gttcttgtgg ttcaacgctt accctgcgca agtgttcatg
     2401 ggtgatgtgg gtgctttggc catgggtgcg gctttgggta cggtggcagt gattgtg
//
LOCUS       DAOMVK010000348         2439 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_12838_length_2439_cov_2.643876, whole genome shotgun sequence.
ACCESSION   DAOMVK010000348 DAOMVK010000000
VERSION     DAOMVK010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12838_length_2439_cov_2.643876"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="PK856_10025"
     CDS             complement(<1..852)
                     /locus_tag="PK856_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRL02745.1"
                     /translation="MFISTHAYSAQVLGQRESETLPTFMQDLQHLRQLQAAWANSKIT
                     QRTAQLLALAERIEANKPELAAAVCMEVGRCLRECEAEIDKTVDLIRYYAKLAPELLD
                     SKTITTQASLSQVRFEPLGVVLAVMPWNYPVWQIMRFAVPALCAGNACLVKPAPSVAQ
                     VTAKLMALVDEAMPLHASYIEHDLIETAIEHADAMAFTGSTDTGRYLASLAGKHIKKC
                     VLELGGSNAFIVLDDADVEQAAHDVVYSRFRDAGQSCNAAKRLIVTPKIADAVVKKVV
                     ALSEQLVM"
     gene            complement(970..1812)
                     /locus_tag="PK856_10030"
     CDS             complement(970..1812)
                     /locus_tag="PK856_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009115371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRL02746.1"
                     /translation="MKTSKIFKSALAVAAVFTLVACSSHTKVSADGSTDEPRWPKAES
                     STFNKNLGIFPDPSSLAQVKAGVTKDQLYYLLGRPHYNEGFVGVREWNYLMHFRTPGQ
                     GTNDVTTCQFKVLFDKKMVAQNFYWNPIDPSTASCPPAVAPATVAAPSTQRYTMNGDA
                     LFAFNRSGTADLLSGARGELDNLASRISKFDRLSSVVVVGHTDRIGSDNYNLSLSQRR
                     AQTVANYLSSRGVPTDVIRAEGVGEAQPVVECSDKLGRSELIACLQPNRRVEIQVTGS
                     GVLN"
     gene            2121..>2439
                     /locus_tag="PK856_10035"
     CDS             2121..>2439
                     /locus_tag="PK856_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRL02747.1"
                     /translation="MLRQVSSYAFMLTALAGLSACGGQSGDAAKPAGNTSAASTSTAD
                     GNVTIMNVSYDVMRDFYKEYNPLFEKEYAAKHGGAKITIEQSHGGSSKQALAVANGLK
                     ADVV"
BASE COUNT          645 a          626 c          582 g          586 t
ORIGIN      
        1 catcaccaat tgctcagaca gtgccaccac ttttttgacc actgcatcgg caatctttgg
       61 cgtcacaatc aaacgcttgg cagcattaca cgattgacct gcgtcgcgaa aacgtgaata
      121 caccacatcg tgcgcggctt gctccacatc cgcgtcatcg agcacgatga aggcattgct
      181 gccgccaagc tccaatacac atttcttgat gtgtttgccc gccaagctcg ccaaatagcg
      241 gcctgtatcg gtagaacctg taaacgccat ggcatcagca tgttctatcg ccgtttcaat
      301 caaatcgtgt tcaatatagc tggcgtgcag cggcatcgct tcgtctacca aagccatcaa
      361 tttggccgtg acttgtgcca cgctgggtgc gggtttcacc aagcaggcat tgcccgcaca
      421 caaagcaggc acggcaaaac gcatgatttg ccacacagga taattccacg gcatcaccgc
      481 caacaccacg cccaaaggct caaagcgcac ttgcgacaaa ctcgcttggg tggtgatggt
      541 tttgctgtcg agcaattcgg gcgcgagctt agcgtaatag cgtatcaagt ccacggtttt
      601 gtcgatttcg gcttcacatt cgcgcagaca gcgccctact tccatgcaca cggcagccgc
      661 caactcgggc ttgttggctt ctatgcgctc ggccaaagcc agtaattgtg cggtgcgctg
      721 ggtgatttta ctgttggccc atgcggcctg tagctgtcgc aaatgctgta aatcttgcat
      781 gaaggttggc aaggtttcgg actcacgttg ccccaagact tgggcactgt acgcatgggt
      841 actgataaac atacaaccac ctttgaaaaa tgacacctca ttatgtgaac ttcaggcaat
      901 taaagcaaga caggcaaaga aaaacccagc cttaagctgg gttggattgt gtacagaagc
      961 ggcctgtaat cagttcaaca caccagaacc tgtcacttga atttccacac ggcgattcgg
     1021 ttgcaagcaa gcaatcaact cactgcggcc caatttgtcg ctgcactcaa ccactggctg
     1081 agcttcaccc acaccttcag cacggatcac atccgtaggc acgccacggc tagacaaata
     1141 attggccacg gtttgggcac ggcgttgtga caaagacaag ttgtaattgt cgctgccgat
     1201 gcggtcggta tgacccacca ccaccactga gcttaaacga tcaaacttgc tgatgcggct
     1261 ggccaagttg tccaattcac cgcgtgcacc actcaacaaa tcggcagtac cagagcgatt
     1321 gaatgcaaac aaagcatcac cattcatggt gtagcgttgg gtgctaggcg cggcaaccgt
     1381 ggcaggtgca actgcaggag ggcaagaagc cgtgcttgga tcgattgggt tccaatagaa
     1441 gttttgtgcc accattttct tgtcaaacaa aactttgaat tggcaagtgg tcacgtcatt
     1501 ggtgccttgt cctggggtac ggaaatgcat caaataattc cactcgcgca cgccaacaaa
     1561 cccttcgtta tagtgtggac ggcccaacaa ataatacaac tggtctttgg tcacgcccgc
     1621 tttaacctgt gccaagctgg atggatcagg gaaaataccg aggtttttat tgaaagtaga
     1681 agattcggct ttgggccagc gtggttcgtc ggttgagcca tcggcactga ctttggtatg
     1741 actggaacag gccaccagag taaataccgc agccacggcc aatgcactct tgaagatttt
     1801 agacgttttc atgacaattc cttgtacaaa ttgaaatagg aaaatggaaa gcatggttaa
     1861 tacatgatga cagatattaa ccgaatacgg cctgcttaaa tagcggtaac gcacaattgg
     1921 gttaaaatgt gttgtaagac tatggtttgc gtcaacccaa attcacaata cacatagtat
     1981 gccaaaaatc agttacaggc cgcttgagct tcattcacat ataatttttt catattaagt
     2041 taagcctatt tgttctttct caaattcaaa caggtattct aagatggcca caaatcaatg
     2101 actttgtggg aataccgttc atgttacgtc aagtttcttc ttatgctttc atgctgacag
     2161 cccttgctgg cttaagcgct tgtggtggtc aatctggcga tgcggccaaa cccgcaggca
     2221 acacgagcgc ggcaagcact tctaccgctg atggcaatgt gaccatcatg aacgtgtctt
     2281 acgacgtgat gcgcgatttc tacaaagaat acaacccatt gtttgaaaaa gaatacgcgg
     2341 ccaaacacgg cggcgcgaaa atcaccatcg agcaatcgca tggcggctca agcaaacagg
     2401 ctttggctgt ggccaatggc ttgaaagccg atgtggtga
//
LOCUS       DAOMVK010000349         2423 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_12947_length_2423_cov_2.315034, whole genome shotgun sequence.
ACCESSION   DAOMVK010000349 DAOMVK010000000
VERSION     DAOMVK010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12947_length_2423_cov_2.315034"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="PK856_10040"
     CDS             complement(<1..858)
                     /locus_tag="PK856_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper ion binding protein"
                     /protein_id="HRL02748.1"
                     /translation="MSDQPLKVPVTQPSNISLPIEGMTCASCVSRVERALKKVPNVLE
                     AHVNLATETAVISMNEPNQSALITAIEDAGYAVPMSTQTFGIEGMTCASCVSRVERAL
                     LKLPNVTTAHVNLANEQATVTGNVTEAELFQAVEDAGYEPHHIAAVATVNEAMLERKA
                     EEQDGLKRDLKLAALLTLPVFVLEMGSHMFMGFHHWIAANIGTQNSWLLQFVLTTLVL
                     LFPGRRFYTKGFPALFKGAPDMNSLVAVGSMAAYLYSLVATFMPQWLPKGTVFVYFEA
                     AAVIVTLILL"
     gene            1034..1231
                     /locus_tag="PK856_10045"
     CDS             1034..1231
                     /locus_tag="PK856_10045"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HRL02749.1"
                     /translation="MKFYIPNMSCGGCARGVTRAIQNVDANAKVNVDLESKWVDVDSS
                     AQANNLAAALTAADFPPQDQA"
     gene            complement(1341..2117)
                     /gene="murI"
                     /locus_tag="PK856_10050"
     CDS             complement(1341..2117)
                     /gene="murI"
                     /locus_tag="PK856_10050"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HRL02750.1"
                     /translation="MNNQYPIGLFDSGVGGTSIWHEVHRLLPHENTVYFADSHNAPYG
                     DKSQDEIIALSKQKVEFLLEKNAKVIVVACNTATTNAMKVLRHDYAVPFVGLEPAIKP
                     AAALTKTGHIGVLATKRTISEQYQLAAESYQNVTVHSQIGYDLVSLIEAGNINTPEMD
                     ALLCQYLNPMLAQDIDQLVLGCTHYPYLKENLQRLLPAHIAIIDSGVAVARQTKCILE
                     AHGLLNDSGKQGQNAFYTNGDLAVLQSFVPNESHVFHQYV"
     gene            complement(2160..>2423)
                     /locus_tag="PK856_10055"
     CDS             complement(2160..>2423)
                     /locus_tag="PK856_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRL02751.1"
                     /translation="DPPSFSNSKKMLEILDVQRDQQRLIDGAMRLLSADGTLFFSNNL
                     RSFELDAAVSERYVVKDVSKQSVPEDFRNKRIHQCWRISHKSA"
BASE COUNT          632 a          608 c          559 g          624 t
ORIGIN      
        1 caataaaatc aaggtcacta tcactgccgc ggcctcgaaa tacacaaaca ctgtaccttt
       61 aggcaaccat tgcggcatga atgttgccac caaagaatac aaatatgcgg ccatgctgcc
      121 cacagcaacc aaagaattca tgtcgggcgc gcctttaaac aaggcgggaa accctttggt
      181 gtaaaagcga cgaccaggaa acagcaacac cagcgtggtg agcacaaact gcagcaacca
      241 actgttttgt gtcccgatgt ttgctgcaat ccaatggtga aagcccatga acatgtgcga
      301 gcccatttcc aacacaaaca ccggcagggt caacaaggcc gccagtttta aatcccgttt
      361 taagccatct tgctcttcag ctttacgctc caacatggct tcattcactg tggcaactgc
      421 ggcaatgtga tgtggctcat agcctgcatc ttccaccgct tgaaacaact cggcttcagt
      481 gacattgcct gtgactgtgg cttgttcgtt tgccaaattc acatgggctg tggtgacatt
      541 aggcagtttg agcaaggccc gctctacccg tgaaacacat gaggcacacg tcattccttc
      601 tatgccaaag gtttgggtac tcattggcac ggcatagcct gcgtcttcaa ttgcggtaat
      661 taaggccgat tgattgggtt cattcatgct gataacggcg gtttctgtag ccaaattcac
      721 atgggcctcc agcacattgg gcactttttt caaggcccgc tctacccgtg acacacacga
      781 agcacacgtc attccttcga tgggaaggct gatgttgctg ggttgggtga cgggaacttt
      841 taagggttgg tctgacatgg cactgtcctt tcaggcttgt tcgatgacat gttggccagt
      901 ctaaacctta ccatgatgac aaggtcaagc ggctaccctg caaatttatt tcggcctgtg
      961 tgcttgacct taccatggtg gcaagcttca tactgcatcc atcacaacaa ccccagtaac
     1021 tgaaaggatg aacatgaaat tctacatacc caatatgtct tgcggcggct gtgcccgtgg
     1081 tgtgacccgc gccattcaaa atgtggatgc caatgccaaa gtcaatgtgg acttagaaag
     1141 caaatgggta gatgtggaca gcagcgcaca agccaacaac ttggccgctg ccctgaccgc
     1201 cgcagacttc cctccacaag accaagccta atgcttacag gccgcttgta tgatccaagc
     1261 ggcctgtaaa tcaaaaacac cagccaattg attgggctgg tgttttttta tcatgccatc
     1321 gcttattcag cgcttgggtt tcaaacatat tggtgaaaaa catggctttc attcgggaca
     1381 aaactttgca acaccgccaa atcaccattg gtgtaaaacg cattttgtcc ttgtttgcca
     1441 gaatcattca gcaagccatg tgcttccaaa atgcacttgg tttgtctggc aacggcaacg
     1501 cctgaatcaa tgatggcaat gtgagcgggt aataagcgct ggaggttttc ttttaaataa
     1561 ggatagtggg tgcagcctag caccagttgg tctatgtctt gtgccaacat gggattcaaa
     1621 tactggcaca ataaagcatc catttcaggc gtattgatgt tgcctgcctc aatcaagctg
     1681 accaaatcat agccaatttg gctgtgaacc gtgacgtttt gataagactc ggctgccaat
     1741 tggtattgct cagaaatggt gcgtttggtg gccaacacgc caatatgccc cgttttggtc
     1801 agcgctgcag cgggtttgat ggcaggctca agccccacaa acggaaccgc ataatcgtgg
     1861 cgcaacacct tcatcgcatt ggtagtggct gtattgcaag ccaccacaat taccttggca
     1921 tttttttcca gcaaaaattc cactttttgc ttggacaagg caatgatttc atcctgactt
     1981 ttgtcaccat acggtgcatt gtgactgtcg gcgaaataga ccgtattttc atgcggcaac
     2041 aagcggtgga cttcgtgcca aatcgaagtc ccgccgacgc cagaatcaaa taagcctatg
     2101 gggtattggt tgttcatttt gcagtgtttg tacacaaaac agggtttaca ggccgcatat
     2161 tatgcgctct tgtgggaaat gcgccaacat tggtggattc ttttattgcg aaaatcttca
     2221 ggcaccgatt gtttggaaac gtctttcacc acatagcgct cagataccgc cgcatccaat
     2281 tcaaaactgc gcaaattgtt agagaaaaac aaagtgccat cggctgacaa caaacgcatc
     2341 gcgccatcaa tcaaacgctg ttgatcgcgc tgtacatcca aaatttccaa catttttttg
     2401 ctattagaaa agctcggtgg gtc
//
LOCUS       DAOMVK010000350         2415 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_12989_length_2415_cov_2.571610, whole genome shotgun sequence.
ACCESSION   DAOMVK010000350 DAOMVK010000000
VERSION     DAOMVK010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12989_length_2415_cov_2.571610"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(193..>1121)
                     /locus_tag="PK856_10060"
     CDS             complement(193..>1121)
                     /locus_tag="PK856_10060"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRL02752.1"
                     /translation="MSAYPFKGLPSLTALRTFESVARLGSAKAASDELFVTPTAISHQ
                     LKALEAQLDVLLFVRLSRQLVLTAAGEQLFEAVHMGMQWMHNGVMALQREHHSNSVIL
                     TTVPNIATRILIPFLGELAVYAPDVALRLHIAHTLSPLDGLQADVAIRYGSGLWPDVD
                     STLLFHNRFVVAASPSLGLKKPTDLLKLPLLHMAWPERVLPPITWSAWKQAANEHGEL
                     DEWDTEAGVVFSDETHAISAAIAGQGVALVSETVVAQELQSGLLQAPLECGLDGCGSH
                     WGGPQSRANEAKLLAVEAWVAAACAAVMGNMT"
     assembly_gap    1122..1131
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1226..1963
                     /locus_tag="PK856_10065"
     CDS             1226..1963
                     /locus_tag="PK856_10065"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRL02753.1"
                     /translation="MFSYHQGEPATFNLSEPLHILQLDASARTGLARQDPHGSQSRAM
                     NHLFANTWQQARPQDSITYRDVGLYPPSALSPAWLEAVFGSDGLHPSEASNRLQESDV
                     LIQELRQSDVLLIATPMYNYGPPAGLKAWLDNIVRINQTFAIQVQEHGLQHVGLLADK
                     PRTAIILSAHGEGGFGPGQPLANINHLHGQITAVLNLIGITDIHVIAQEYEEVGGEML
                     AQSVAAAETAILELATQLSMFTPVLTA"
BASE COUNT          618 a          674 c          529 g          584 t
ORIGIN      
        1 gcaccgcgca aatgagctgg caacctgaag gctggggcga caacagcgcg tggttgcaca
       61 tgttcgtcaa tgctcgcaag gcatttgatt aatcgcgttt taagtcagta aaaaagcggc
      121 ctgtaaactt tacaggccgc tttttcgctt tgttcacatc acaaacccat taatacatgt
      181 gttttatttg tcttaagtca tatttcccat cacagccgca caagccgcag ccacccaagc
      241 ctctacggct agcagcttcg cttcattggc ccgcgactgg ggcccccccc aatggctgcc
      301 acaaccgtcc aagccacact ccaaaggcgc ttgcaacaga cctgactgca gctcttgcgc
      361 caccactgtc tcgctcacca aggccacacc ctgccccgca atggccgcac tgatggcatg
      421 ggtttcatcg gagaacacca ctcctgcttc agtgtcccat tcatccagtt caccatgctc
      481 attggcggcc tgcttccaag cactccacgt tatcggtggc aacacccttt ctggccatgc
      541 catgtgcagc aaaggcagtt tgagcaagtc tgttggcttt tttaatccca agcttggact
      601 ggctgccacc acaaagcggt tgtgaaacaa caaagtgctg tcaacatcag gccacaatcc
      661 tgagccgtag cgtatggcca catcggcctg taagccatcc aaaggcgata aggtatgggc
      721 tatgtgcaag cgcaaagcca catcgggtgc atacacagcc agctcgccca agaaaggaat
      781 caaaatgcgc gtggcaatgt tgggcacggt ggtgaggatg acgctgttgc tgtggtgctc
      841 tcgttgcaag gccatgacac cattgtgcat ccattgcatg cccatgtgca cggcctcaaa
      901 cagctgttca cccgcagctg ttaacaccaa ttgcctcgac aagcgcacaa acaataacac
      961 atccaattgg gcttccaagg ctttcaattg gtgactgatg gcagtaggcg tcacaaacaa
     1021 ctcatcactg gcggctttgg cactgcccaa acgtgccaca gactcaaatg ttctcaatgc
     1081 cgtcagcgat ggcaggcctt tgaatggata cgcagacatg tnnnnnnnnn ngatgaattt
     1141 aattcaatca aattaaaatt tttatcaatt gtagcatcga ttacagatga ttaatattca
     1201 tccatctcaa cccagaaagc acaccatgtt cagctaccac caaggcgagc ctgccacatt
     1261 caatttgtcc gagcctttgc acatcttgca attggatgcc agtgccagaa caggattggc
     1321 ccgtcaagac ccgcatggtt cgcaaagccg tgccatgaac catttgtttg ccaacacgtg
     1381 gcagcaagcc agaccacaag acagcatcac gtaccgcgat gtcggcctct acccgccttc
     1441 agcattgagc ccagcttggt tggaagcggt atttggctcc gatggcttgc acccaagcga
     1501 agcatccaat cgcttgcaag aaagcgatgt gctgatacaa gaattgcgcc agtccgatgt
     1561 cttgctgatt gccacgccca tgtacaatta cggcccacct gctggattga aagcctggct
     1621 agacaacatt gtgcgcatca accaaacctt cgccatccaa gtccaagagc atggcttgca
     1681 gcatgtgggc ctgctcgccg acaaaccgcg cacagccatc atcttgtcgg cacacggtga
     1741 agggggtttt gggccaggtc agcctttggc gaacatcaat cacttacacg gccaaatcac
     1801 cgccgtattg aatttgatag gcatcactga catccatgtc attgcacagg aatatgagga
     1861 ggtcggtggt gaaatgttgg cacaatcggt agccgcagca gaaaccgcca tacttgaatt
     1921 ggcaactcag ttatccatgt tcactcccgt cctgacagca tgaacagtat gaacagcgct
     1981 tcatcatgga cttaaccaac cctgatgtaa gctcagattc aaagcaataa atcaaaaaat
     2041 gcggcctgta catgtttaca ggccgcattt taatgttgca aatgcattgc gtggctttga
     2101 ttaggttgat tgaagtcgtt cagtcatgag cattctggtg ccgatgtgtc caaagttacc
     2161 cacccaatag tcacggcaaa cccattgcca gtcatctcgt cattcttatt acttgtagct
     2221 tttgtacgca tactttgagt taaaagggtt cgtcactgcc tttaatccaa gttgacgatt
     2281 taactcatcg tttcaaaatt tcttatttat cacatctttg ttgccacttt taccacaggt
     2341 ttgcacattc attgccacac ccctatttaa attgcataca gacatcctta ctggtcacac
     2401 cttacaagcc ttcat
//
LOCUS       DAOMVK010000351         2403 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_13056_length_2403_cov_2.869676, whole genome shotgun sequence.
ACCESSION   DAOMVK010000351 DAOMVK010000000
VERSION     DAOMVK010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13056_length_2403_cov_2.869676"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PK856_10070"
     CDS             complement(<1..325)
                     /locus_tag="PK856_10070"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HRL02754.1"
                     /translation="MKPLTMQGKTEFTQFWHCPELGGLEVLKAQYQHKEFSKHVHEGY
                     CINLIENGAQAFYRSGHMHVAPQGDIVLVNPDEVHTGSSAVEHGWGYRALYPTPDMIA
                     HISQDF"
     gene            complement(481..1713)
                     /locus_tag="PK856_10075"
     CDS             complement(481..1713)
                     /locus_tag="PK856_10075"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase family
                     protein"
                     /protein_id="HRL02755.1"
                     /translation="MSVFTPLHLNNGTTLPNRIAKAAMEENMADADHAPSAALIRLYQ
                     SWAQGGAGLLITGNVMVDRTAMTGAGGVVLENAQFLPRFQRWAQAAQQGGGQVWMQLN
                     HPGRQMPQNLGQITVAPSAVALNVGSLSKQFALPQALSEAQVEDIIARFVNSAQLAEQ
                     AGFDGVQIHAAHGYLLSQFLSPLSNVRTDTWGGSLANRARLLLEIVRQVRAAVSADFA
                     VAVKLNSADFQRGGFSAEDAKTVVQWLGDLQVDVVELSGGSYEAPAMQGQTRDGRTLA
                     REAYFLEFARDIAQVATMPLMVTGGIRRLEIAEQVLASGVALVGMATALALQPDLPRT
                     WRECNSTAAPMLRPIAWRNKALASAANMAVVRQQLKRLSRGAVPKANASAAWALLCNQ
                     WEMRGRSQQYRAWVTAKP"
     gene            1793..>1978
                     /locus_tag="PK856_10080"
     CDS             1793..>1978
                     /locus_tag="PK856_10080"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRL02756.1"
                     /translation="MNIGELSRLSGVPAPTIRFYEKIALLPKAKRHANGYRYYSEEDV
                     SWLSLIQRAQQVGFALEE"
     assembly_gap    1979..1988
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1989..2202
                     /locus_tag="PK856_10085"
     CDS             <1989..2202
                     /locus_tag="PK856_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02757.1"
                     /translation="KALLPLGEQAKQHETMLPRLQQKMAEMDAQIAQLLADKQKLQAV
                     VDAIINKPADMTCSENAQRLLKQAQP"
BASE COUNT          537 a          624 c          681 g          551 t
ORIGIN      
        1 aaaaatcttg gctgatgtgg gcaatcatgt cgggcgtggg gtagagggcg cgataacccc
       61 aaccatgctc caccgccgac gagcctgtgt gcacctcatc gggattgacg agcacgatat
      121 cgccttgcgg cgccacgtgc atgtggccac tgcggtaaaa cgcttgtgcc ccattttcaa
      181 tgaggttgat gcaatagcct tcgtgcacat gtttggaaaa ttctttgtgt tggtattggg
      241 ctttcaatac ctccaaaccg ccgagctcgg gacaatgcca aaattgggtg aattcggttt
      301 tgccttgcat ggtgagtggt ttcaatcacg ccgtaaaacc ctgtttctac cgcactgacg
      361 cgcaggcgtc tagaacaaaa ttgcttgcta gcgctttgaa aaaccaaaca agcggcctgt
      421 aactatttac aggccgcttg ttggtgaaca tagccattca aatggagtgc gtgtgtcgat
      481 ttaaggcttg gcggtcaccc acgcgcggta ttgttggctg cggccacgca tttcccattg
      541 attgcacaac aaggcccaag cggcagatgc gttggctttg ggcactgcgc cgcggctgag
      601 gcgtttgagc tgttgtctga ccacggccat attggcagca gaagccagcg ccttgttgcg
      661 ccaagcaatc ggcctgagca tgggtgcggc cgtgctgttg cactcgcgcc aagtgcgtgg
      721 caagtcgggt tgcaaggcca aagcggtggc catgcccacc aaggccacgc cactggccag
      781 cacttgttca gcgatttcca agcggcggat gccacctgtg accatcagtg gcatggtggc
      841 gacttgggca atgtctcggg cgaattccaa aaaatacgct tcgcgcgcca aggtgcgacc
      901 atcacgggtt tgcccttgca tggcgggtgc ttcgtagctg ccgcctgaca attccaccac
      961 atcgacttgc aaatcgccca gccactgcac cacagttttg gcatcttcgg cactgaagcc
     1021 gccgcgttga aaatcggccg aattgagttt gactgcgact gcaaagtcgg cagacacggc
     1081 agcgcgcact tggcgcacga tttccaatag caatctggcg cgattggcga gcgagccgcc
     1141 ccacgtatcg gtgcgcacat tgcttaaggg tgataaaaat tgactgagca aatagccgtg
     1201 tgcggcatgg atttgtacgc catcaaaacc tgcttgttcg gccaattggg cgctgttgac
     1261 gaagcgcgca atgatgtctt cgacttgcgc ctcgctcaag gcttgcggca aggcaaactg
     1321 tttggacaag gagcccacat tcaaggccac ggccgaaggg gccacagtga tttggcctaa
     1381 attctgtggc atttgtcgcc cagggtgatt gagttgcatc cacacttggc cgccgccttg
     1441 ttgggctgcc tgtgcccaac gttgaaaacg aggcaagaat tgggcatttt ccaataccac
     1501 gccgcctgcg cccgtcattg cggtgcggtc taccatcacg ttgccagtga tgagcaagcc
     1561 tgcgccgcct tgggcccagc tttggtacaa acggattaag gccgcactgg gcgcgtggtc
     1621 ggcatcggcc atgttttctt ccattgcggc ttttgcgatg cgatttggca aggtggtgcc
     1681 gttgttcagg tgtaaggggg taaaaacaga catggtctgg cctcattcaa tgcaaaatta
     1741 agatgaggtg catggtaaga ttgaagctca ctttaaggtc aagaggtgac ccatgaacat
     1801 tggtgaattg tcgcgtttga gcggtgtgcc tgcgcccacc attcgctttt acgaaaaaat
     1861 tgcgctcttg cccaaggcca aacgtcatgc caatggttac cgttattaca gtgaggaaga
     1921 tgtgtcgtgg ctgtctttga tacagcgcgc acagcaagtg ggctttgcct tggaggaann
     1981 nnnnnnnnca aggctttgct gcccttgggt gagcaagcca agcaacacga aacgatgttg
     2041 ccgcgtttgc aacaaaaaat ggctgaaatg gatgctcaaa tcgcccaatt gctggcagac
     2101 aagcaaaaac tgcaagcggt ggtggacgcc atcatcaaca aacctgcgga catgacgtgc
     2161 tcagaaaatg cgcagcgttt gctcaaacaa gcccagcctt aatgcttgat acattgaaaa
     2221 agcggcctgt aaatgtttac aggccgcttt tgtgatggca ggcagtgttg ccacagggat
     2281 tacgcattca acgccaatac gggtattacc aagtcttctt catcggccaa gtggcggtag
     2341 agcagcgtgc cttgggtttg cacggcggtg tgtaaacgct ccaccagatc caattgtggc
     2401 tcg
//
LOCUS       DAOMVK010000352         2403 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_13059_length_2403_cov_2.037905, whole genome shotgun sequence.
ACCESSION   DAOMVK010000352 DAOMVK010000000
VERSION     DAOMVK010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13059_length_2403_cov_2.037905"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1935)
                     /locus_tag="PK856_10090"
     CDS             complement(<1..1935)
                     /locus_tag="PK856_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02758.1"
                     /translation="MSQISVTSETLEPTATQTPPPKKPVSKWRWLKRAVWALMSVLIV
                     LMLALLWLTKTESGLRFTLFRIPAWFGVEITADQVSGSVWSGFKGENWVVKTEGANIN
                     LRNLNIDWASSELFSRTIHVRKIDIGEMHIDVMAAEPRPDQESTFPQSLRLPANVHLD
                     EVNIAKITMGKNHDVILNQAKLNYHYQHGQPHRLVIDKLDSTFGIADGHFSLGESKPF
                     ATDAKLNVLGSRDGITVKGGVGISGDLDNTVIKGKFSGQGVNLDIDGKLHPFAKTLNE
                     QFDHLNIAGEGLNPKTIIPTLPEAELKLNLAIEPIAGSTELSGRLNVSNGKPLPADGN
                     GIPVKSIVSQFKLDHAGKIVVAPSSITTLQKGIVTAQGTVDVPEQQLNLNVGLASMTL
                     ADVIQNPMAGTLNGDIAVTGKFDTPQADWQLKTERLQSTGRFNMVKDRDNGQQSLKIS
                     KARIATPDGGAIGVAGTMNLYKEIKLALVSHRFNPAALSKDYPQGNVNLDARVMGTLV
                     GEPVIRSILDLGDSTLSGAKLVGNGDVIFQQQHLSRANLNVLLDGNRIKTDGSIGKAG
                     DKLNVDVHAPRLDQFGFGLSGLLTAKGHVAGDPKKLDIQLAGNAQGLKFQDDVHVQQL
                     RFDVSASPDMAAPMKVDIHGN"
     gene            complement(2000..>2403)
                     /locus_tag="PK856_10095"
     CDS             complement(2000..>2403)
                     /locus_tag="PK856_10095"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRL02759.1"
                     /translation="YGTFIVRGQGGLVNVSNELNAPQSLLFRTGGANSVRGYEYESIG
                     IRGYEDAVIGGKVMATASLEYQLPIKQDYALAVFHDMGGVSNSIGDFTIKHGTGIGGR
                     WFSPFAPIAFDLAYGHEDQKIRWYIGLGTQF"
BASE COUNT          564 a          647 c          577 g          615 t
ORIGIN      
        1 gttgccatgt atgtccactt tcatcggcgc ggccatgtct ggcgacgcgc tcacatcaaa
       61 acgcaattgc tgcacatgga catcatcttg gaacttcaaa ccttgggcat tgccagccaa
      121 ttggatgtcg agttttttcg ggtcgcccgc cacatggcct ttggctgtga gcaagccact
      181 caagccaaaa ccaaattgat ccaagcgtgg cgcatgcaca tccacattca atttatcccc
      241 cgccttgccg atgctgccat cggttttgat gcggttgccg tctaacaata cgttgaggtt
      301 ggcacggctc aaatgctgtt gctgaaaaat cacatcacca ttgcccacca gtttcgcgcc
      361 cgacaaagtg ctgtcgccca aatcgagaat gctgcgtatc accggttcac ccacaagcgt
      421 gcccatcacg cgggcatcca aattgacatt gccttgtgga tagtctttgg acaaggccgc
      481 aggattgaag cggtgactga ccaaagccag tttgatttct ttgtacaaat tcatcgtgcc
      541 cgccacacca atggcgccac catcaggtgt ggcgatgcgg gctttgctga ttttcaagct
      601 ttgctggcca ttgtcacggt ctttcaccat gttaaagcgg cctgtagact gcaaacgctc
      661 ggttttgagc tgccaatcgg cttggggcgt gtcaaatttg cctgtgacag caatgtcacc
      721 attgagcgtg cctgccatcg ggttttgtat cacgtcggcc aaagtcatgc tggccaagcc
      781 cacgttcaaa ttcagctgtt gctcaggcac atccaccgtg ccttgtgcgg tgacaatgcc
      841 tttttgcaaa gtggtgatgc tggaaggcgc caccacgatt ttgcctgcat ggtcgagttt
      901 gaattggctg acaatggatt tcacaggaat gccattgcca tcggcaggca atggtttgcc
      961 attgctcaca ttcaatctgc ccgataattc tgtgctgcct gcaatcggct ctatcgccaa
     1021 attcagcttc aactcggcct caggcaaagt tgggatgatg gtttttgggt ttaaaccctc
     1081 gcctgcgatg ttcaaatggt cgaattgttc gttcaaggtt ttggcaaacg ggtgtagctt
     1141 gccatcaatg tcgagattca cgccttgacc gctgaatttg cctttgatga cggtattgtc
     1201 caaatcgcca ctgatgccca caccaccttt gacggtaatg ccatcacggc tgcccaacac
     1261 attcaatttg gcatcggtgg caaacggttt gctctcgccc aaagaaaaat ggccatcggc
     1321 aatgccaaac gtgctgtcga gtttgtctat gaccaaacgg tgtggctgtc catgttggta
     1381 gtggtaatta agcttggctt ggttcaaaat cacatcgtgg tttttgccca tggtgatttt
     1441 ggcgatgttg acctcatcca aatgcacatt ggctggcaaa cgcaaacttt gaggaaaagt
     1501 cgattcttgg tcaggacgcg gctcggctgc catcacatca atgtgcattt cgccgatatc
     1561 gattttgcgc acatggatgg tgcgggaaaa caattcgctg ctggcccaat caatgttcaa
     1621 attgcgcaaa ttgatgttgg caccttcggt tttcaccacc caattctcgc ctttgaaacc
     1681 gctccatacc gagcctgaca cttggtcagc ggtgatttcc accccaaacc atgcagggat
     1741 gcgaaacaag gtgaagcgca aacccgattc ggttttggtg agccacaaca gcgccaacat
     1801 caacactatc agcacagaca tcagcgccca cactgcgcgc ttgagccacc gccatttgga
     1861 aacaggtttt ttaggcggcg gtgtttgggt tgctgtgggt tctaatgttt cactcgttac
     1921 actgatttgg ctcatacacc gtactcaata ctctgataaa tggacacaga ctcatgcggc
     1981 ctgtaaagac aaatgggggt taaaattggg tacccaaacc gatgtaccaa cgaatctttt
     2041 gatcttcgtg gccataggcc aaatcaaagg caatcggcgc aaacgggcta aaccaacggc
     2101 caccaatgcc agtgccatgc ttgatggtaa agtcacctat gctgttcgac actccgccca
     2161 tgtcatggaa taccgccaag gcgtagtctt gtttgatggg caattggtat tccaagctgg
     2221 cagtggccat cactttgcca ccaatcaccg catcttcata accgcgtatg cctatgctct
     2281 catactcata accgcgcacc gaattggcac caccggtacg gaataacaaa gattgcggcg
     2341 cattcaattc attgctgaca ttgaccaagc cgccctgccc gcgcacgatg aaagtaccgt
     2401 att
//
LOCUS       DAOMVK010000353         2398 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_13092_length_2398_cov_2.105847, whole genome shotgun sequence.
ACCESSION   DAOMVK010000353 DAOMVK010000000
VERSION     DAOMVK010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13092_length_2398_cov_2.105847"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PK856_10100"
     CDS             complement(<1..183)
                     /locus_tag="PK856_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009116300.1"
                     /note="with YejAEF is involved in resistance to microcin
                     C; Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin ABC transporter permease"
                     /protein_id="HRL02760.1"
                     /translation="MWTYIFKRVLLLIPTLLGILALTFAIIQFVPGGPVEQMVQQLNH
                     GSQGMAEVSSGGGMHAG"
     gene            complement(280..705)
                     /gene="cueR"
                     /locus_tag="PK856_10105"
     CDS             complement(280..705)
                     /gene="cueR"
                     /locus_tag="PK856_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006878 - cellular copper ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HRL02761.1"
                     /translation="MNIGAAAKASGVSAKMIRYYEEQGLIVAAKRTDAGYRIYSEQDV
                     QVLRFIKRARDLGFAVEWIKSLLALWQDQHRQSSDVRELAKRHVDELQQKITQLQQMV
                     GTLQTLIDCCAGDARPDCPILADLEQIHFAGEKPKPSCH"
     gene            990..2216
                     /locus_tag="PK856_10110"
     CDS             990..2216
                     /locus_tag="PK856_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL02762.1"
                     /translation="MSVTKSSLNRLTILFPLALVLFEFSVYIGNDLIQPAMLSITKEF
                     GVSSSWAPSSMSFYLLGGACVAWLLGPLSDRIGRKKVMLVGVAFFVLTCLGILFTHNI
                     EQFLLLRLLQGTGLTVISAVGYAAIQETFEERDAIKVMALMANISLLAPLLGPILGAF
                     LIDHVSWHWGFIGIAFLAFLSWFGLNSKMPDHPPVAAKMPVKALFAEFKQVYANRRFL
                     GLTLSLPIISLPLMLWIALSPVILVEELDLSSMEYGLAQFPVLGGLILGNILLVRIVD
                     KMALGRSVLMGLPIMLVGTLIILIGTAVSSVFIPCLILGMSLISFGEGIAFSVTYRFA
                     LMSSEVGKGTVAASVSMLLTLVFFFVIEIVRVLYEHLGLWVYSLACFALIALWFTFPR
                     RMLKAIMQERKTQNIF"
BASE COUNT          524 a          634 c          527 g          713 t
ORIGIN      
        1 gcctgcgtgc ataccgccac cactgctgac ctcggccatg ccttggctgc catggttcag
       61 ctgctgcacc atttgctcaa caggcccacc tggtacgaat tgaatgatgg caaacgtcaa
      121 agccaaaata ccgagcaaag tcggtatcaa cagcaacacc cgcttgaaaa tataagtcca
      181 catattgacc cgtcattagc gcaacaggtc tatcttagca tgcccaagcc gcgtgtctgc
      241 aaggaatgct tacaggccgc ttgttccatt gcggcattgt taatggcagc tgggtttagg
      301 tttttcacct gcaaagtgaa tttgctccaa atctgccaga atcgggcagt cgggtctggc
      361 atcacccgca caacaatcga ttaaggtttg caaagtgccc accatttgct gcaattgggt
      421 aattttttgc tgcaattcat ccacatggcg tttggccaac tcgcgcacat cgctgctttg
      481 acggtgttgg tcttgccaca aagctaataa ggatttgatc cactccacag caaaacccaa
      541 atcccgtgcg cgcttgataa agcgcaaaac ttgcacgtct tgctcgctgt aaatgcggta
      601 gccagcatcg gtgcgtttgg ctgccacaat caagccttgc tcttcataat accgtatcat
      661 tttcgctgac acgcccgatg ctttggctgc tgctcctatg ttcatgcctt catcctcaat
      721 aaggaaacct tccttgttca aaccttgttt acaggccgct tatcatcggc ccataaacca
      781 ctcatgattg catgtttcta caagcggcct gtaaaacccg ctttgacaag ccgcttgcta
      841 tcacctatgc tttgccctga aaccaaatgt ttcaaatttt taagcttatt tcttcttggc
      901 tttctgcgcc aaacaccgtg ttgtttcttg aagctgtcgc agctttaagg gttccgtttt
      961 accgtttacc ttcatctata aagcatttca tgtctgttac aaaatccagt ttaaaccgcc
     1021 tcaccatctt attcccattg gctttggtgt tgttcgagtt ttccgtatac ataggcaacg
     1081 atttaatcca acctgccatg ctctccatca ccaaagaatt tggggtatcg agctcgtggg
     1141 cgccttcttc catgtcgttt tacttgctgg gcggcgcttg cgtggcctgg ctgctagggc
     1201 ctttatcaga ccgcataggc cgcaaaaaag tcatgttggt cggcgttgca ttctttgtgc
     1261 tcacttgctt gggcatcttg ttcacccaca acatagaaca gtttttactg ctgcgcctgt
     1321 tgcaaggcac agggctgacg gtgatttcag ctgtaggcta tgccgccatt caagaaacat
     1381 ttgaagagcg cgatgccatc aaagtgatgg ctttaatggc caacatctca ctgctcgccc
     1441 ccttgcttgg ccctatcctg ggcgcgtttc ttatcgacca tgtgtcatgg cactggggct
     1501 ttataggtat tgcgtttctg gcgtttttaa gctggtttgg cctgaacagc aaaatgcccg
     1561 atcacccacc tgtggcggca aaaatgcctg taaaagcctt gtttgcggaa ttcaagcaag
     1621 tctatgccaa ccgccgcttc ttgggcttaa ccctgtcctt gcccataatc agcctgccct
     1681 taatgctgtg gattgccttg tctcccgtga tattggtaga agaattggac ttgagcagca
     1741 tggaatatgg cttggcccaa tttcctgtac ttggaggctt gatattaggc aatattctgt
     1801 tggtgcgcat tgttgataaa atggctttgg gccgctctgt tttgatgggc ttgcccatca
     1861 tgctcgtggg caccctcatc atcctcatcg gtacggcagt ttccagcgtg ttcatcccat
     1921 gcttaatatt gggcatgagc ctcatcagtt ttggcgaagg catagctttt tccgttacct
     1981 accgttttgc cttgatgtct tccgaagtcg gcaaaggcac ggtagccgcc tctgtttcca
     2041 tgctgctgac gctggtgttc ttttttgtca ttgaaatcgt gcgcgtttta tacgagcact
     2101 tgggcttgtg ggtttacagc ctggcttgtt tcgctttgat tgcactgtgg tttaccttcc
     2161 cacgccgcat gctcaaagcc atcatgcaag agcgcaaaac ccaaaacatt ttctaagcat
     2221 ggcaaatcat ttaagcggcc tgtaagtcaa aacattggtt tacaggccgc ttttttaacc
     2281 attggtctgt gtatcaagcc aatgacggcc tatcaagtca catgaactgt gttaacactt
     2341 tattcttgca ccatgttcaa aatcaattca tcccactttt tcattccacc cacttggc
//
LOCUS       DAOMVK010000354         2398 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_13094_length_2398_cov_1.999146, whole genome shotgun sequence.
ACCESSION   DAOMVK010000354 DAOMVK010000000
VERSION     DAOMVK010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13094_length_2398_cov_1.999146"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PK856_10115"
     CDS             complement(<1..421)
                     /locus_tag="PK856_10115"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HRL02763.1"
                     /translation="MMTWQFEDDLGVCHVRVSQRGKTQIKLRVRERHLIEISAPKHVT
                     AKRIQTFLSEHQHWLYQQRAQLSHITEPEHIWYQGQRLPIIMRPVNTAISLQTDACIV
                     SNALAQPYQTLLQHLQTQAQAQLLPRLKSWLTPPHTDL"
     gene            complement(418..1182)
                     /locus_tag="PK856_10120"
     CDS             complement(418..1182)
                     /locus_tag="PK856_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007341830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRL02764.1"
                     /translation="MILLKNLLYWLCVSLFTVVHFCYIVVCLAFGVKAANRACTQWAL
                     ALVWLLEHIIGLKYEVKGLDNIPDEPVVICCKHQSGWETLILQKFFRPIVFVAKKELF
                     QIPFFGWGLKLAGTIGIDRKDRLGASRQLLEIGGLRKKQGFSIAIFPEGTRIKPGLRG
                     RYKLGGARIANTLEMPIVPVALNSGEFWPKNSFMKYPGTISVVIGKPINPVGQGPEAV
                     MQQVETWIESQQRVIEGRGPFANKSVAAEQDERTGS"
     gene            complement(1182..1766)
                     /gene="gmhB"
                     /locus_tag="PK856_10125"
     CDS             complement(1182..1766)
                     /gene="gmhB"
                     /locus_tag="PK856_10125"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003822145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HRL02765.1"
                     /translation="MTKLVILDRDGVINEDRDDFVKSVDEWVALPGSMDAIAFLNQAD
                     FRVVVATNQSGIGRGFFTIHDLNEMHAKMHRLAQQAGGQIEAVWFCPHTAADKCACRK
                     PLPGMVEDILQRYLNTDPDELYLVGDSLRDLQAIEAVGGQPVLVLTGKGKKTVADPAI
                     PEDTLIFHDLLEFAQTMLQNHQKRQAVQTEQEEQ"
     gene            complement(1763..2224)
                     /locus_tag="PK856_10130"
     CDS             complement(1763..2224)
                     /locus_tag="PK856_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02766.1"
                     /translation="MEFEIIDHEEWHTEEDFPADLSPVHALTHMGMYFEWAAHKQLLH
                     PDVAAQLAPALEKLATRELTGRDIVEQALGKQLLLSFFNELGQRFSAYYYADDDEGYG
                     QFLSDYVNTLRTDLLPSFYHVDNSQAAYDAIASQFDIAYARWSDSLRDAAS"
BASE COUNT          571 a          615 c          614 g          598 t
ORIGIN      
        1 ccaaatcggt gtggggtggg gtgagccaag attttaagcg cggcaacagt tgggcttggg
       61 cttgggtttg caaatgctgt aataaggttt ggtaaggctg ggcgagggcg ttggatacga
      121 tgcacgcatc tgtttgcaag ctgatggctg tgtttacagg ccgcatgatg atgggcaagc
      181 gttgcccttg gtaccaaatg tgttcaggct cggtgatgtg agacagttgt gcacgttgct
      241 ggtagagcca gtgttggtgc tcggagagga aggtttggat gcgtttggca gtgacgtgtt
      301 tgggggcgct gatttcaatc aagtggcgct cacgcaccct gagtttgatt tgggttttgc
      361 cgcgctggct cacgcgcacg tggcacacac ccaaatcatc ctcaaattgc catgtcatca
      421 tgagccggtg cgttcgtctt gctctgcggc cactgatttg ttggcaaacg ggccgcggcc
      481 ttcgatgacg cgctgttggc tttcaatcca tgtttccact tgctgcatca ccgcttcagg
      541 gccttgaccc acggggttga tgggcttgcc tatgaccaca ctgatggtgc ctgggtattt
      601 catgaacgag tttttaggcc aaaactcgcc gctgttcaac gccacgggaa cgatgggcat
      661 ttccaaagtg ttggcaatgc gtgcgccgcc caatttgtag cgaccgcgca aacccggctt
      721 gatgcgcgtg ccttctggga agatggcaat cgagaagcct tgttttttgc gcaagccacc
      781 gatttccagt aattggcgcg acgcgcccaa gcggtctttg cggtctatgc ctatggtgcc
      841 tgccagtttc aagccccagc caaaaaatgg aatttgaaac agttcttttt tcgccacaaa
      901 cacgatgggg cggaagaatt tctgcaagat aagggtttcc cagcccgatt ggtgtttgca
      961 gcaaatcacc actggctcat caggaatgtt gtccaagcct ttgacttcgt atttcaggcc
     1021 gatgatgtgt tccaataacc acaccaaagc caaagcccat tgcgtgcacg cgcggttggc
     1081 ggctttcacg ccaaaagcca aacacaccac gatgtaacaa aaatgcacca ccgtaaacag
     1141 gctgacacac agccaataca gcaaattttt caataaaatc atcattgttc ttcttgctcc
     1201 gtttgcaccg cttggcgttt ttgatggttt tgtaacatcg tttgtgcaaa ctcaagtaaa
     1261 tcatggaata ttaaagtgtc ttcaggaatg gcagggtcgg caacggtttt cttgcctttg
     1321 cctgttaaca ccaacaccgg ttggccaccc acagcctcaa tcgcttgcaa atcgcgcaag
     1381 ctgtcgccca ccaaatacaa ctcatcaggg tcggtgttca aatagcgctg caaaatgtct
     1441 tctaccatgc caggcaaagg cttgcgacac gcgcatttgt cggccgctgt gtgtgggcaa
     1501 aaccacaccg cctcaatttg accgcccgct tgttgcgcca aacggtgcat tttggcgtgc
     1561 atctcattca aatcatgaat ggtgaaaaaa ccacggccta tgcccgattg gttggtggcc
     1621 accaccacgc gaaaatcggc ctgattcaaa aaggcaattg catccatgct gccaggtaag
     1681 gccacccatt catcgaccga tttcacaaaa tcatcgcggt cttcattgat gacgccatcg
     1741 cggtctaaaa tcaccaattt ggtcatgatg ccgcatcccg taaactgtcg ctccagcgtg
     1801 cataggcaat gtcaaattgt gaagcgatgg catcgtaagc cgcttggctg ttgtccacgt
     1861 ggtaaaacga gggcagtaaa tcggtgcgca aggtgttgac gtaatcgctt aaaaattggc
     1921 cataaccctc gtcatcatcg gcgtagtaat acgcgctgaa gcgctggccc aactcattaa
     1981 aaaagctgag caacaactgc ttgcccaaag cctgttccac tatgtcgcgg ccggtcagct
     2041 cgcgtgtggc caatttttcc aaagcagggg ccaattgcgc cgccacatca ggatgcaaca
     2101 attgcttgtg cgcggcccat tcaaaataca tgcccatgtg agtgagtgcg tgtactggcg
     2161 acaagtccgc aggaaagtct tcctcggtat gccattcttc atggtcgatg atttcaaatt
     2221 ccatattcag ctcaagtgca gtcggaaaag gtcaaaacta taacattgtg acggtgtgca
     2281 tgcacacaca ttaatgtgtg gcgcacactg taaaccacga ttaaaccatg tgttttattt
     2341 gatgccacag tcttttaata aagccgcggt ttcaaacaaa ggcaagccca tcacgccg
//
LOCUS       DAOMVK010000355         2396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_13106_length_2396_cov_3.095258, whole genome shotgun sequence.
ACCESSION   DAOMVK010000355 DAOMVK010000000
VERSION     DAOMVK010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13106_length_2396_cov_3.095258"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..122)
                     /locus_tag="PK856_10135"
     CDS             complement(<1..122)
                     /locus_tag="PK856_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003762738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase"
                     /protein_id="HRL02767.1"
                     /translation="MDIIFLNGMAVETLVGVYEWERQHSQTLYLDLQVGIVENS"
     gene            186..791
                     /gene="plsY"
                     /locus_tag="PK856_10140"
     CDS             186..791
                     /gene="plsY"
                     /locus_tag="PK856_10140"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HRL02768.1"
                     /translation="MVDILVVVLAYLLGSLSFAVIVSKAMGMADPRSYGSGNPGATNV
                     LRSGKKKAAILTLLGDALKGLVAVLLAKYFLDAGSITEATVAWVALAVLIGHMWPVFF
                     GFKGGKGVATAVGVLFALSWPVALICTLVWLVMAFGLKISSLAALTATVLSPVLAWVF
                     IDETSWCWAVLIIAILVLYRHKSNIQNLLSGKETKIGGSSE"
     gene            950..2176
                     /locus_tag="PK856_10145"
     CDS             950..2176
                     /locus_tag="PK856_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HRL02769.1"
                     /translation="MHQLKLAVSGAQILFVAFGALVLVPLLTGLNPAMALLGAGVGTL
                     LFQLLTKQQVPVFLGSSFAFIAPIIYANQTWGMGATMFGLFCTGFMYFVFALMIRFGG
                     IEAVNKLLPPVVIGPVIMVIGLSVAVVACQMAMGQEGGKQVMDYPKSLLLAGFTFLVT
                     MVVTVFAKGMMRLVPILIGVAAGYALALVMGLVNTAKLINAPLFAIPEFHTPEINWHA
                     ALFMLPVAIAPAIEHIGGIMAIGKVTGKDYTQTPGLHKTLAGDGLGVCFAGLIGGPPV
                     TTYGEVTGAVMITKNGNPVIMTWAAIFAIAMAFFGKFNALLGSIPMPVMGGIMILLFG
                     TIASLGLKTLIDAKVDLMNPKNLVIVSTVLTTGVGGMLVKIGSFSFAGVGLCAVLAIV
                     LNLIIPNREQTEEVSS"
     gene            complement(2179..>2396)
                     /locus_tag="PK856_10150"
     CDS             complement(2179..>2396)
                     /locus_tag="PK856_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HRL02770.1"
                     /translation="KIDVPAGKSVTLQPGGFHVMLMGLKKPLKLNDTFPVTLKYQSGR
                     TQKITATVNNGSGMQTMNHGEHHDHQM"
BASE COUNT          504 a          535 c          617 g          740 t
ORIGIN      
        1 tggctgtttt caacaatgcc cacttgcaaa tccagataca gggtttggct gtgttggcgt
       61 tcccactcat acacacccac caaagtttcc accgccatgc catttaaaaa gatgatatcc
      121 attgtgccca gcactttctc cgcgtaaaat tttcgttttg gcattttaat gaaaaagcga
      181 tgggtatagt agatattttg gtggtggtgc tggcgtattt gctgggctca ctgtcttttg
      241 cagtcattgt gtccaaagcc atgggcatgg ccgatccacg cagttatggc tcaggcaatc
      301 ctggtgccac caatgtgtta cgcagtggca agaaaaaagc cgcgattttg actttattgg
      361 gtgatgcctt aaaaggcttg gtggcggtat tgctggccaa gtatttttta gatgccggca
      421 gcatcactga agcgactgtc gcttgggtgg cactggcggt attgattggt cacatgtggc
      481 ctgtgttctt cggcttcaaa ggcggtaaag gtgtggccac agcggtaggc gtgttgttcg
      541 ccttgtcttg gccagtggca ttgatatgta ccttggtttg gcttgtcatg gcatttggct
      601 tgaagatttc atctttggcg gccttaacgg ccaccgtctt gagtcctgtc ttggcttggg
      661 tgttcattga tgaaactagc tggtgctggg cggttttaat cattgccata ctggttttat
      721 accgacacaa aagcaatata caaaacctct tatcgggtaa agagaccaaa attggcggtt
      781 cttctgaata gatgaaacgg tgatacaagc ggcctgtaag cttacaggcc gcttttttgt
      841 gtctttacta gctgtattgc cacttaaaag ctgcgataat tcgcaacgtt aaattttctt
      901 aagattttta gccccattgt tttgtatttt ccattaagga cacgttgtca tgcatcagct
      961 taaattggct gtttcaggcg cacaaatttt gtttgtcgcc ttcggcgcct tggtcttggt
     1021 tcccttgctt acgggattga atccagccat ggccttactc ggtgccggtg tcggtacttt
     1081 gttgtttcaa ttattgacca aacaacaagt accggttttt ttgggttcct cattcgcctt
     1141 catcgcaccc atcatttatg ccaaccaaac ttggggcatg ggtgccacca tgttcggttt
     1201 gttttgcacg ggcttcatgt attttgtgtt tgcgctgatg atccgttttg gtggcattga
     1261 agcggtgaac aaattgttgc cacctgtggt gattggccct gtcatcatgg tgattggttt
     1321 gtcagtggcc gtagtcgctt gtcaaatggc catgggccaa gaaggtggta aacaagtgat
     1381 ggattacccc aaatccttgc tgttggctgg cttcaccttt ctggtgacga tggtggttac
     1441 tgtgtttgcc aaaggcatga tgcgcttggt acccatttta atcggtgtgg ccgcaggtta
     1501 tgctttggcc ttggtcatgg gcttggtcaa taccgccaaa ctcatcaacg cgcccttgtt
     1561 tgccattcct gaatttcaca cccctgaaat caactggcat gcggctttgt tcatgctgcc
     1621 cgtggcgatt gcccctgcga ttgagcacat cggtggcatc atggccatcg gtaaagtaac
     1681 gggtaaagat tacacacaaa cacctggttt acacaaaacc ttggctggcg acggcttagg
     1741 tgtgtgcttc gcaggtttga taggcggccc acctgtgacc acttatggtg aggtaacagg
     1801 tgcagtgatg attaccaaaa atggcaatcc cgttatcatg acttgggcag caatatttgc
     1861 cattgccatg gctttctttg gcaaattcaa tgccttgctc ggctctattc ccatgccagt
     1921 gatgggcggc atcatgattt tgttgtttgg tacgattgcg tctttgggct tgaaaaccct
     1981 gattgatgcc aaagtggact tgatgaaccc gaaaaacttg gtcatcgtca gcaccgtgtt
     2041 gaccacgggt gtgggcggta tgttggtgaa aattggcagc tttagctttg cgggcgtggg
     2101 cttgtgcgcg gtattggcga ttgtgttaaa cctcatcata cctaaccgcg aacaaaccga
     2161 agaagtgagc tcttaaactc acatttgatg atcgtggtgt tcaccatgat tcatggtttg
     2221 catgccgctg ccgttgttca cggtggcggt aattttttgg gtgcgtcccg attggtattt
     2281 caaggtcact ggaaacgtat cgttcagttt caacggtttc ttcaaaccca tcaacatcac
     2341 atgaaagccg cctggttgca acgtcacgct tttgccagca ggcacgtcta ttttgg
//
LOCUS       DAOMVK010000356         2393 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_13125_length_2393_cov_2.533362, whole genome shotgun sequence.
ACCESSION   DAOMVK010000356 DAOMVK010000000
VERSION     DAOMVK010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13125_length_2393_cov_2.533362"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="PK856_10155"
     CDS             <1..413
                     /locus_tag="PK856_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRL02771.1"
                     /translation="GHPVSAAVALKNVEILQDEKVIQNVHDVTGPYMQQKWRDMLAQF
                     KYVDDVRGVGLIQGFTLVKNKATREMFPNEGETGTALRDIFFRNNLIMRACGDHIVAS
                     PPLTITKEEIDLMLATAEKCIKEFEANMDAKLGA"
     gene            complement(795..1817)
                     /locus_tag="PK856_10160"
     CDS             complement(795..1817)
                     /locus_tag="PK856_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02772.1"
                     /translation="MHSQSPWIATPSKDLWIVLPPFVVVAIALLFHTHLATIESKYSW
                     WTWLVLIVLVDVAHVYASIFRTYLLPQAWAKQRTLYVGIPLLCLILSMLLYQAGAAVF
                     WSVLAYVAVFHFVRQQWGLMRLYGRFDPKTKLGSVIDALVIYTGTVYPMLYWMISADR
                     QFVWFVDHEFVTLFSPQILPILAAVYLVIIGVYVVRAMAQCFTGRFNLPKNLIIIGTY
                     LSWYVGIVHYNHPLIFTLLNVVSHGVPYMALVYFADISGKSRQFTLVGKLLSWQGLCV
                     YLAILLGLALMEEVLWELAVWGEHFSDEFLLDATWLWLVVPLLALPQLTHYVLDGFIW
                     RKPKRP"
     gene            complement(1874..>2393)
                     /locus_tag="PK856_10165"
     CDS             complement(1874..>2393)
                     /locus_tag="PK856_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02773.1"
                     /translation="DLLYFDIDIVITGDITPLLKAQQFTMLSGLSYKNQCNSSLMYIP
                     CSIKKHVWETFMSNPQQHMDECVVPEKWGDQGFISAITEPKEWQKELPNTIYSYKIDI
                     ATPTMLGYDAVRSNGTGTGVPPKGAILVCFHGYPSPWKTGLSWVPYFSLKEKVSGKMK
                     AVRSWMKAKRQH"
BASE COUNT          731 a          601 c          491 g          570 t
ORIGIN      
        1 caggtcaccc tgtttcagcc gctgtggcct tgaaaaacgt ggaaattttg caagatgaaa
       61 aagtcattca aaacgttcac gatgtgactg gcccatacat gcagcaaaaa tggcgtgata
      121 tgttggcgca attcaaatac gtggacgatg tgcgcggcgt gggcttgatt caaggcttca
      181 ccttggtgaa aaacaaagcc acacgtgaaa tgttcccaaa tgaaggcgaa acaggaacgg
      241 ctttgcgcga cattttcttc cgcaacaatt tgatcatgcg cgcctgtggc gaccacatcg
      301 tggcatcgcc tccattgacc atcaccaaag aagaaattga tttgatgttg gccacggcag
      361 aaaaatgcat caaagaattt gaagccaata tggatgccaa attgggtgct taaacacctg
      421 attgcatgaa tataagcggc ctgtaaatgt ttacaggccg cttttttgtc tgtgtgcaaa
      481 tccacccact actttgtttt acgtcgcaat gactccaaga tttccaagcc caatagcaaa
      541 gtgatttcgt ctgtaatctg attcaagtca acgccaattt cattcctaat cttcaagata
      601 agctcagaac gagacatttc gatttgattg gcgacatgat tcaaacaaga tcgcaaatgc
      661 aaacctcgat gcgataaggg taaactgata tccaaaatct tgcgcttatg cgaattaaaa
      721 gacatagctc acctcatttt caaattcaaa cgaacccagt atttgagtgc caccatgttc
      781 atctcaaagc agcatcatgg tcttttgggc ttgcgccaaa taaacccatc caacacataa
      841 tgcgttaatt gcggcaaagc cagcaaaggc acaaccagcc ataaccacgt tgcatccaac
      901 aaaaactcat cactgaaatg ctcgccccaa acagccaact cccacaacac ttcctccatc
      961 aatgccaaac ccagcaatat cgccaaatac acgcacaaac cctgccaaga aagcaatttt
     1021 ccaaccagag taaattggcg ggatttgcca ctgatgtcgg caaaatacac caaggccatg
     1081 taaggtactc catgtgacac cacgttcaat aaggtaaaaa tcaacgggtg gttataatgc
     1141 acaatgccga cataccaaga taagtaagtg ccaatgatga tgaggttttt gggcagatta
     1201 aagcggcctg taaagcattg tgccattgcc cgaaccacat acacaccgat gatgaccaga
     1261 tacactgctg ccaagatggg caaaatttgc gggctgaata aggtgacaaa ctcatggtca
     1321 acaaaccaaa caaactgcct atcggcactg atcatccaat acagcatcgg gtacacagtc
     1381 ccagtgtaaa tcaccagtgc atcaatcaca ctgcccagct tggttttggg gtcaaagcgg
     1441 ccatacagcc gcatcaaacc ccattgctga cgcacaaaat ggaacaccgc cacataagcc
     1501 aataccgacc aaaaaactgc ggcacctgct tggtataaaa gcatgctcaa tatcagacac
     1561 aacagcggaa tgcccacata caatgtccgt tgtttggccc aggcctgagg caataaatat
     1621 gtgcgaaaaa tggacgcgta cacatgagcc acatcaacga gcacaatcaa caccaaccac
     1681 gtccaccacg agtatttcga ttctatcgtt gccaagtgcg tgtgaaacaa caaggcaatc
     1741 gccaccacca caaacggtgg caacacgatc cataagtctt tacttggcgt ggctatccat
     1801 ggcgactggc tgtgcatgac actccttcat taatggccgt cttgaatgaa aacggtttct
     1861 gtgcccatgg ccattaatgc tgtctcttgg ccttcatcca agagcgcaca gccttcattt
     1921 ttcccgacac tttctctttc aatgagaaat aaggcaccca tgacaaacct gttttccaag
     1981 ggctggggta gccatgaaaa cacaccagta tcgctccttt aggaggaacc cctgtaccag
     2041 tgccatttga gcgcacagca tcgtagccga gcatggttgg cgtagcgata tcgattttgt
     2101 aactgtaaat ggtattgggc agctcttttt gccattcttt gggttcggta atggcactga
     2161 tgaaaccttg gtcaccccat ttctcaggca ccacacactc atccatgtgt tgttgtgggt
     2221 tggacataaa ggtttcccac acatgttttt tgattgaaca aggaatgtac atcaatgaag
     2281 agttgcattg atttttgtaa gacaaacccg acaacatggt aaattgctgt gcttttaata
     2341 gcggcgtgat gtcacccgtg atgacgatat caatgtcaaa atacaataaa tct
//
LOCUS       DAOMVK010000357         2382 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_13202_length_2382_cov_3.426730, whole genome shotgun sequence.
ACCESSION   DAOMVK010000357 DAOMVK010000000
VERSION     DAOMVK010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13202_length_2382_cov_3.426730"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="PK856_10170"
     CDS             <1..636
                     /locus_tag="PK856_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HRL02774.1"
                     /translation="YSLPTPNGVKVSIMLEELGLPYDAHTVSFERQDQMSAEFLSLNP
                     NNKIPAILDPNGPDGKPLQLFESGAILWYLAEKTGQFIPQDAARRYECMQWLYFQVGG
                     VGPMMGQVGFFHKYAGKDYEDKRPLERYVAESKRLLGVIDQHLRGKQWMMGDEYTVAD
                     IAILPWLRNLNEYYDAGHLVNFNSFAEVNRVLANWLKRPAVQKGLEIPPRA"
     gene            complement(719..1102)
                     /locus_tag="PK856_10175"
     CDS             complement(719..1102)
                     /locus_tag="PK856_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02775.1"
                     /translation="MKYLAILGLMCALPVASQAAITYNNEWKAGGAQSFQHTLEAPAG
                     KYRLEIQTSSDAPIAVYSGFKGNKSAPLMQLNQQKKGNSGSAEIELRPTKPDHAYGYA
                     PITVRVGGAKNTHSGSYQLQLTPLF"
     gene            1317..>2382
                     /gene="speA"
                     /locus_tag="PK856_10180"
     CDS             1317..>2382
                     /gene="speA"
                     /locus_tag="PK856_10180"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009119185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HRL02776.1"
                     /translation="MSWSIEDAASVYGINHWGDGYFSINAQGHVAVKPRADSEQAIDV
                     YDLVAQVNQRGQDLPMLFRFPDILQDRVKRLCSAFDKAIDKYDYDAQYTAIYPIKVNQ
                     QESVVKNIIATHEQGVSIGLEAGSKPELMIVLAFAPRGGTIVCNGYKDREFIRMALIG
                     QKLGHNVFIVIEKESEVDLIIEEAKHMGMCANLGLRVRLSSLASSKWADTGGDKGKFG
                     LSASQLISAQKKLQAAGLADYVRLMHFHMGSQIANIADYRLGFREALRYFAELRALGL
                     PIETIDVGGGLGVDYDGTHSRNASSINYSMSEYAHIIVSSLQEFCEENNLAVPNIFSE
                     SGRAMTAHHAVLVMNVTDVET"
BASE COUNT          580 a          572 c          611 g          619 t
ORIGIN      
        1 tattctttgc ccacgcccaa tggggtgaag gtgtcgatta tgttggaaga attgggtctg
       61 ccttacgatg cgcacacggt cagctttgag cgccaagatc aaatgtcggc agaatttttg
      121 tctttaaacc caaacaacaa aatccctgcc atcctcgacc caaacggccc cgatggcaaa
      181 cctttgcaat tgttcgagtc aggcgcgatt ttgtggtatt tggctgaaaa aacgggtcaa
      241 ttcatcccgc aagatgctgc ccgtcgctat gagtgcatgc aatggctgta tttccaagtt
      301 ggtggtgtgg gcccgatgat gggacaagtg ggctttttcc acaaatacgc gggcaaagac
      361 tatgaagaca agcgcccgtt ggagcgctat gtcgccgaga gcaaacgctt attgggcgtg
      421 atagaccaac atttgcgcgg caaacagtgg atgatgggcg acgaatacac tgtggccgac
      481 atcgccattt tgccgtggct gcgcaatttg aatgagtatt acgatgcggg gcatttggtg
      541 aatttcaatt cgtttgccga ggtcaatcgc gtattggcca actggctcaa gcgccctgcg
      601 gtacaaaaag gcttggaaat cccgcctcgc gcctaaacct atatcattga ctagtgatga
      661 acaagcggcc tgtaagcttt atcaagttta caggccgctt atttggtcat gacgggtttc
      721 agaacaaagg cgtgagttgc aattggtaac tgccactgtg ggtattttta gcgccaccta
      781 cccgtaccgt aatcggagca tagccataag catggtcggg tttggtgggt cgcaattcaa
      841 tttcagcgct gccactgttg ccttttttct gttggttcag ttgcatcaag ggtgccgatt
      901 tattgccttt gaaaccactg taaactgcga tgggtgcatc gctgctggtt tgtatttcca
      961 agcggtattt tcctgcgggt gcttctaagg tgtgttgaaa gctttgggct ccgcccgctt
     1021 tccattcatt gttataggtt atggcggctt gactcgccac aggtaacgca cacatcaagc
     1081 ccaaaattgc caagtatttc atgtttgagt ctccttttct tcaccacaat tgttctgcct
     1141 aaatgacaga taactcgcca ctggagttgc atttttaaac gcatttaatc atatgcttac
     1201 ctagatttac ctgaggcaca gggtaaaatt taatgacctg ttggcacaca agccgcacac
     1261 aagggagttt ttacgttacc atgcgcccca tgaaacctac gattaaaggg caaagcatga
     1321 gttggagtat tgaagacgcg gccagtgtct acggcatcaa ccattggggc gacggctatt
     1381 tcagcatcaa tgcccaaggc catgtggcgg tgaagccacg tgccgattca gagcaagcca
     1441 ttgatgtgta cgacttggtg gcacaagtga atcaacgtgg tcaagatttg cctatgctgt
     1501 ttcgctttcc cgacattttg caagatcggg tgaaacgctt gtgcagcgca ttcgataagg
     1561 cgatagacaa atacgattac gatgcccaat acactgccat ttatccgatt aaggtcaacc
     1621 aacaagaatc ggtggtgaaa aacatcatcg ccacccatga gcaaggcgtg tccattggtt
     1681 tggaagcagg ctcaaaaccc gaattgatga ttgtgttggc gtttgcgccg cgcggcggca
     1741 ccattgtgtg caatggctac aaagaccgcg aattcatccg catggctttg ataggccaaa
     1801 aacttggcca caatgtgttc atcgtcatcg aaaaagaatc cgaagtcgat ttgatcattg
     1861 aagaagccaa acacatgggc atgtgcgcca atttgggctt gcgcgtgcgt ttgtcttctt
     1921 tggcttcgag caaatgggcc gacacgggtg gcgacaaagg caagtttggc ctgtctgcca
     1981 gccaattgat ttcggcgcaa aagaaattac aggccgctgg tttggccgat tatgtgcgct
     2041 tgatgcattt ccacatgggc tcacaaatcg ccaacatcgc tgattaccgt ttgggctttc
     2101 gcgaagcgct gcgctatttc gccgaattac gtgccttggg cttgccaatt gaaaccatag
     2161 acgtgggcgg cggcttgggt gtggactacg atggcacgca ctcacgcaat gccagctcca
     2221 tcaattactc catgagcgaa tacgctcaca tcattgtcag cagcttgcaa gaattctgtg
     2281 aggaaaataa tttggccgtg ccaaacattt tctccgaatc tggccgcgcc atgactgccc
     2341 accacgccgt tttggtgatg aacgtcaccg atgtggaaac ct
//
LOCUS       DAOMVK010000358         2376 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_13235_length_2376_cov_3.933649, whole genome shotgun sequence.
ACCESSION   DAOMVK010000358 DAOMVK010000000
VERSION     DAOMVK010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13235_length_2376_cov_3.933649"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /gene="ligA"
                     /locus_tag="PK856_10185"
     CDS             complement(<1..977)
                     /gene="ligA"
                     /locus_tag="PK856_10185"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRL02777.1"
                     /translation="MTDESIQQQMDELIHLLERYNREYHELDEPSVSDAEYDVAFRHL
                     QQLETQHPHLMRPVSPTQRVGGKALSQFDSVVHQVPMLSLNNAFSPVNVDGVYDHSEM
                     QAFAERVHSGLGHDPEYFIEPKFDGLALSLVYENGVLLQAATRGDGSEGENVTNNVKT
                     IRSIPLHLNTPTPPARLEVRGEVLMFKADFDALNERQAAEGGKAFANPRNAAAGSLRQ
                     LDAKITAKRTLRFFAYSIAQLDEAYAQATHGLELDLLASWGIPTPPRDCCLQSHDMAQ
                     INQFYEQMAIKRPELPFDIDGMVIKVNAIAEQEVLGFVSRAPRFAIAHK"
     gene            complement(994..2283)
                     /locus_tag="PK856_10190"
     CDS             complement(994..2283)
                     /locus_tag="PK856_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HRL02778.1"
                     /translation="MSDTTFYYIIGGLAAILAVLLFNSYKEKRYRDEVRRQFGHSDQD
                     ALLDGKREEVRDGQGVLNQGMMHHQHSQAAADEALFDVEPDTVNKIQAAVEQDAVVFD
                     VVNPTAEFEPEATEPVENEAQPVASAPKSIKDLAVPGIKNVLSAANKSEAGLLLDLQD
                     LTRMELPWFDNRVDYMAYVSLREARELHTMPRLSNRHHFRVVGCTMDGRFQLAEPIPS
                     VYYQGFVMGLQCISRKGLAAREDLTQFAEQVRQFASQMGAGVAVANADAFMQVAEPMD
                     ELCVRVDQTIAIHLVSRESVSGREIKAALESLKFELDAGIFWYRDAHGKNLFNAVNLD
                     STPFIAAALDDQAYRGFSMLYDLTKVPAGEKTFNQFMDLVVKLSSHLGLDLVDDQLNE
                     LSTQWLKDIRSYVVERQDEMLSVDIEPGSELAERLFS"
BASE COUNT          559 a          669 c          565 g          583 t
ORIGIN      
        1 aatttgtgcg caatcgcaaa acgcggcgca cgcgacacaa agcccaagac ctcttgttcg
       61 gcgatggcat tgactttaat caccatgcca tcaatgtcaa acggcaattc aggccgctta
      121 atcgccattt gttcgtaaaa ttggttgatt tgcgccatgt catggctttg caaacagcaa
      181 tctcgtggcg gcgtgggtat gccccaagac gccagcaaat ccagctccaa accatgcgtg
      241 gcttgagcat aagcctcatc caattgggcg atgctgtaag cgaaaaagcg caaggtacgt
      301 ttggcggtga ttttggcatc gagttggcgc aagctgcccg ccgcggcatt acgcggattg
      361 gcaaacgcct taccgccctc ggccgcttgg cgctcattca aggcatcgaa atcggccttg
      421 aacatcaaca cctctccgcg cacttccaaa cgtgccggtg gcgtgggcgt gttcaaatgc
      481 aatggaatgc tgcgtatggt tttgacattg ttggtgacat tttcgccctc gctgccatcg
      541 ccacgggtag cggcctgtaa taacacgccg ttttcataca ccaagctcaa ggccaagcca
      601 tcaaatttcg gctcgataaa gtattcgggg tcatgtccca aacctgagtg cacgcgctcg
      661 gcaaaggctt gcatctcgct gtggtcgtac acaccatcca cattcacagg cgaaaatgca
      721 ttgttcagac tcagcatcgg cacttgatgc accacgctgt caaattgact taaggccttg
      781 ccacccacac gttgcgtggg gcttacaggc cgcatcaaat gtgggtgttg tgtttccaat
      841 tgttgcaaat gccgaaacgc cacatcgtat tcagcatcgc tgacgctggg ttcatccagc
      901 tcatggtatt cgcggttgta gcgttcaagc agatgaatca attcgtccat ctgttgttga
      961 atggattcat cggtcatgtg acatccttat cactcaagaa aataagcgtt ctgccagctc
     1021 actgcctggc tcgatgtcca cgctgagcat ctcgtcttgg cgctccacca catagctgcg
     1081 gatgtctttt aaccactgtg tcgacaattc attcaattgg tcgtccacca aatccaagcc
     1141 caaatggctg gacagcttca ccaccaaatc catgaactga ttgaaggttt tttcaccggc
     1201 aggcactttg gtcaaatcgt ataacatgga gaagccacgg taggcttggt catccaaagc
     1261 cgcagcaata aacggcgtgc tgtccaaatt caccgcattg aacaggtttt tgccatgcgc
     1321 atcgcggtac cagaaaatgc ccgcatccaa ttcaaatttc aggctttcca aagcggcttt
     1381 gatttcacgt ccgcttaccg actcgcgtga caccaaatgg atggcaatgg tttggtccac
     1441 gcgcacacac aattcgtcca tgggctcggc cacttgcatg aaggcatcgg cattggccac
     1501 cgccacgccc gcccccattt ggctggcaaa ttgacgcact tgctcggcaa actgtgtcaa
     1561 atcctcacgc gccgccaagc ctttgcggct gatgcactgc aaacccatca caaagccttg
     1621 gtaatacacg cttggtatcg gctctgccaa ttggaaacgg ccatccatgg tgcaaccaac
     1681 cacgcggaaa tggtggcgat tgctcaaacg tggcatggtg tgcagctcgc gcgcttcgcg
     1741 caaagacaca tatgccatgt aatcgacacg gttatcaaac cacggcaatt ccatgcgggt
     1801 taagtcttgc aaatccagca acaaccctgc ttccgattta ttggcggccg ataacacatt
     1861 tttaatccct ggcacggcca agtctttgat gcttttcggt gcacttgcca ctggctgagc
     1921 ttcgttttcc acaggctcag tcgcttctgg ctcaaactca gcagtcgggt tgaccacatc
     1981 aaacaccacc gcgtcttgct caaccgctgc ttgtatcttg tttaccgtgt ctggctcaac
     2041 atcgaaaagc gcttcatcag cagccgcttg gctgtgttgg tggtgcatca tgccttgatt
     2101 gagcacgcct tgaccgtcgc gcacttcttc acgcttgccg tctaataaag catcttggtc
     2161 tgaatgccca aattgacgtc tgacctcatc acgatagcgt ttttctttgt aagaattgaa
     2221 cagcaatacc gccaaaatgg cagccaagcc gccgatgatg taatagaaag tggtgtcgct
     2281 cataggtgat gaatcatcct tggttaactc ggatggccgc aagctcggcc agtttgctca
     2341 agagtatagc ggaagttaat gcaattttca gccgcc
//
LOCUS       DAOMVK010000359         2366 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_13311_length_2366_cov_3.030290, whole genome shotgun sequence.
ACCESSION   DAOMVK010000359 DAOMVK010000000
VERSION     DAOMVK010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13311_length_2366_cov_3.030290"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="PK856_10195"
     CDS             <1..637
                     /locus_tag="PK856_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HRL02779.1"
                     /translation="VHLSRLSEELILWMSPRVGFIDIADRFCTGSSIMPQKKNPDVPE
                     LVRGKTGRVVGHLMGMIVLMKSQPLAYNKDNQEDKEPLFDTVDTLVDTLRIYADMMRG
                     VTVKADNMRAAVMQGFAMATDLADYLVKKGMPFRDSHEVVARAVRFADDKGVDLSELA
                     FADLAQFSDLIAEDVFEHLTPEGALNARDHIGGTAPAQVRAQIARWRGLIA"
     gene            complement(820..1683)
                     /locus_tag="PK856_10200"
     CDS             complement(820..1683)
                     /locus_tag="PK856_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02780.1"
                     /translation="MTETEPKWTWIPNERLGNLYFERAYADFDAFIKDKLQWEHEDYC
                     DDDEAIYQQREGGDFFTFCGETHIFDGAEINSALYYRGQNLIGLTLAAFKSCFAADND
                     WELDGWDECHTSELLGVFVHLSDGKVESLYYYKDCFPNEQAQNATINTAEPPWTWCPN
                     LRVSDLYFEQAYANFDAFIADKQFDPGHGCPDDDISAYRQTASGDYFVFQGKEHLFVN
                     AIIYSETHYQSQNLIGLTLSAFKSCFAEIDDWKITEQNECHTSEALNAVMWLEDGKVA
                     SVSLSGDCADD"
     gene            complement(1686..1973)
                     /locus_tag="PK856_10205"
     CDS             complement(1686..1973)
                     /locus_tag="PK856_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02781.1"
                     /translation="MQKALTKVELTNILFISSDFVIQFFEQIQTLKPVAFLKTANQYN
                     VSVSSTKDTNTACKQFTGRLLDKTHHGKTISVQNLFIEVNYNQLLSVYFRG"
     gene            2114..>2366
                     /locus_tag="PK856_10210"
     CDS             2114..>2366
                     /locus_tag="PK856_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRL02782.1"
                     /translation="MSEQAIVIGAGMVGVSVAWHLQQQGMQVTLIDRKQPGEETSYGN
                     AGLIQREAVFPHAFPRDVKDIFKVIPNHNLDIRYRPAALW"
BASE COUNT          628 a          541 c          535 g          662 t
ORIGIN      
        1 ggtgcatttg tcgcgtttgt cagaagaatt gattttgtgg atgagcccac gcgtaggctt
       61 tatcgacatt gcagaccgct tctgcacagg ctcgtccatc atgccacaaa agaaaaaccc
      121 agacgtgccc gaattggtgc gtggcaaaac aggccgtgtt gtgggccatt tgatgggcat
      181 gatagtgttg atgaaatcac agcctttggc ttacaacaaa gacaaccaag aagacaaaga
      241 acctttgttt gacacggttg acaccttggt ggacaccttg cgcatttacg ccgacatgat
      301 gcgcggcgtc actgttaaag ccgacaacat gcgcgccgcc gtgatgcaag gcttcgccat
      361 ggccaccgat ttggccgact atttggtgaa aaaaggcatg cctttccgcg acagccacga
      421 agtggtggcc cgtgccgtgc gctttgccga cgacaaaggc gtggatttga gcgaattggc
      481 atttgccgat ttggcccaat tcagcgattt aatcgccgaa gacgtgtttg aacatttaac
      541 ccctgaaggc gctttgaatg cacgcgacca catcggaggc accgcgcctg cgcaagtacg
      601 cgcccaaatc gcccgctggc gtggtttgat tgcttaaggt cttacatgta agtcatcgtt
      661 agaatcaaaa agcggcctgt aaatcagttt acaggccgct ttttatcatc tgcattgcaa
      721 tctatggatt ttttgtattg tagcttgaat aacaaaagac atactgtcat gttgatcaat
      781 gaggctggtt tttctttagc cgctgtaagg ttaccatacc taatcatccg cacaatcgcc
      841 actaagactc actgatgcta ctttaccatc ttctagccac atcactgcgt tcaatgcctc
      901 agatgtatgg cactcattct gttctgttat tttccaatca tcgatttcgg caaaacagga
      961 tttaaatgct gacagggtga ggcctatcag gttttgactt tggtaatggg tttctgagta
     1021 aatgatggca ttgacaaaca ggtgttcctt gccttgaaac acgaaataat caccgcttgc
     1081 agtttgtctg taggcagata tgtcgtcgtc tgggcagcca tggcctggat caaattgttt
     1141 gtcggcgata aaagcatcaa aattggcata ggcttgttca aaatacaaat cactcacgcg
     1201 caggttgggg caccaagtcc atggcggctc tgctgtattt atcgtggcgt tttgtgcctg
     1261 ctcattgggg aaacagtctt tgtagtaata cagtgattcc actttgccat cgcttaagtg
     1321 cacaaatacg cccaataact cagaggtatg gcattcatcc caaccatcca attcccaatc
     1381 attgtctgct gcaaagcagg atttgaatgc tgccaaagtg aggccaatca ggttttgacc
     1441 tctgtaatac agggcagagt tgatttcagc accgtcaaat atatgtgttt caccgcagaa
     1501 agtgaaaaaa tccccacctt ctcgttgctg atagatggct tcatcgtcat cacaatagtc
     1561 ttcgtgttcc cattgcaact tgtcctttat aaaagcatcg aaatcggcat aagcccgttc
     1621 aaaatacaaa ttacccagtc gttcattggg tatccacgtc catttcggtt ctgtttcagt
     1681 catcattacc ccctgaaata aactgataga agttgattgt aattgacttc aataaacagg
     1741 ttttgcactg agattgtttt accatgatgc gttttatcca acaagcggcc tgtaaactgt
     1801 ttacaggccg tatttgtgtc ttttgtagaa gacacgctga cgttatactg atttgcagtc
     1861 tttaagaagg caacgggctt tagtgtttga atctgctcaa aaaattgaat cacaaaatcg
     1921 ctgctaataa ataatatatt agttaattca acttttgtta acgctttttg cattgcatta
     1981 tctaattaat ctagaaaatt acttcttaat atcacttttt taaatacagt aagcgctata
     2041 atggcgcatc ggccgccaca ttgcatttgg cgcgtcacat cttcaacaag agaaaagtag
     2101 caagggcaat aaaatgagcg aacaagcaat cgtcataggc gcgggtatgg tgggcgtgtc
     2161 tgtggcgtgg catttgcaac aacaaggcat gcaggtaacc ttaattgacc gcaaacaacc
     2221 gggtgaggaa acctcatacg gcaatgcggg cttaatccag cgtgaggcgg tgtttccgca
     2281 tgcgtttccg cgcgatgtga aagacatatt caaagtcatc cccaatcaca atttggacat
     2341 ccgttaccgt cctgcggcct tgtggc
//
LOCUS       DAOMVK010000360         2350 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_13425_length_2350_cov_2.179085, whole genome shotgun sequence.
ACCESSION   DAOMVK010000360 DAOMVK010000000
VERSION     DAOMVK010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13425_length_2350_cov_2.179085"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1642
                     /locus_tag="PK856_10215"
     CDS             <1..1642
                     /locus_tag="PK856_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HRL02783.1"
                     /translation="FEAAAVIVTLILLGRYLEARAKGRTSEAIQRLVGLQAKTARVER
                     NGEMVELPIAEVKLGDIIEVRPGERIAVDGMVLSGQSFVDEAMISGEPVPVEKKEGSP
                     VTGATVNQNGSLRYEATAVGETTVLAQIIRMVSDAQGGKLPIQTAVDKVTLWFVPAVM
                     VLAALTFLAWWIWGPDPRLTFALVNAVAVLIIACPCAMGLATPTSIMVGTGRGAEMGV
                     LFRRGEALQGLQEVNVVAVDKTGTLTEGKPVLTDFIAAQGFERAQVLPLVAAAENRSE
                     HPIARAIVVAAEGMKLPELIDFQSITGLGIAATTAEHKVEVGADRYMHSLGLDVSVFQ
                     ADAERLAGQGKSPMYAAIDGKLVAIMAVADPIKESTPAAIQALHDMGLKVVMISGDNQ
                     HTAKAIARQLNIDEVVAEVMPEGKVDTLKTLQKQGRVAFIGDGINDAPALAQADVGLA
                     VGTGTDIAIEAADVVLMSGRLTAVSDAIALSRATIRNIRQNLFWAFAYNVALIPVAAG
                     VLYPKFGILLSPMFAAGAMALSSVFVLSNALRLRRFKP"
     gene            complement(1702..>2350)
                     /locus_tag="PK856_10220"
     CDS             complement(1702..>2350)
                     /locus_tag="PK856_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02784.1"
                     /translation="ARLLLSPKKILLLDEPFAGLDKKSREALWQMLVYNTVLVTCFTF
                     TLHGLLQLKWPWQADNLRLLGMHTFFSLLTFAVYWARKEKILQTLFVTAHGGEWLRID
                     ARGLTWQDDGNEWLSINPVGLEWRAMSGQRLFFADQIPHTFHIAWADILEVQLHKISR
                     RHYEINIISSKPYNTLGHTEYTVTSFGQFRSIFILHALLSHLHRLALNAHKKSRP"
BASE COUNT          556 a          542 c          670 g          582 t
ORIGIN      
        1 tttcgaggcg gccgccgtga tagtgacttt gattctattg ggccgttatt tggaagcacg
       61 cgccaaaggc cgtacttctg aagccataca gcgtttggta ggcttgcaag ccaaaacagc
      121 acgggtagag cgcaatggtg aaatggtgga gttgccgatt gccgaagtga aattgggcga
      181 catcatcgaa gtgcgacctg gtgagcgcat agccgtagac ggcatggttt taagcggcca
      241 gtcgtttgta gatgaggcga tgatttcggg cgaacccgtg ccagtggaga aaaaagaggg
      301 ttcacctgtg acaggtgcaa ccgtcaacca aaacggcagc ttgcgttatg aagccacggc
      361 agtgggcgaa accaccgtgt tggcgcaaat cattcgcatg gtcagcgatg cgcaaggcgg
      421 caaattaccg atacaaaccg ctgtcgataa agtcacctta tggtttgtgc ctgcggtgat
      481 ggtgttggcg gccttaacct tcttggcttg gtggatttgg gggcctgatc cacgcttgac
      541 ttttgctttg gtcaacgcgg tggcggtgtt aatcatagcc tgtccttgtg ccatgggttt
      601 ggccacgccc acatccatca tggtaggcac aggccgtggt gccgagatgg gggtgttgtt
      661 ccgtcgtggt gaggctttgc aaggtttaca agaagtgaat gtggtggcag tagacaaaac
      721 aggcacttta accgaaggca aacccgtgtt aaccgacttc attgcggccc aaggttttga
      781 gcgtgcacag gtcttgcctc tggtggcggc agcagaaaac cgctccgagc acccgattgc
      841 ccgtgccatt gtcgtcgcag cagaaggcat gaaactgcct gagttaatcg attttcaatc
      901 cataacaggt ttgggcattg ccgcgaccac tgctgaacac aaagtggaag tgggcgcaga
      961 ccgctacatg cacagcttgg gcttggatgt cagtgtgttt caggccgatg ctgaacgttt
     1021 ggcagggcaa ggtaaatcac ctatgtatgc cgcaatagac gggaagctcg tcgccatcat
     1081 ggctgtggcc gacccgatta aagagtcaac gccagcagcc attcaagcct tgcatgacat
     1141 gggtttgaaa gtggtgatga tttcgggcga taaccaacac actgccaagg cgattgcccg
     1201 tcaattgaac atagacgaag tggtggccga agtgatgcct gaaggtaagg ttgatacctt
     1261 gaaaacctta caaaagcaag gtcgggtagc attcattggc gatggcatca acgatgcccc
     1321 tgctttggca caggccgatg tgggcttggc ggtaggcaca ggtactgaca ttgcgattga
     1381 agcggcggat gtggtgttga tgtcgggccg attaaccgca gtttcagatg cgattgcctt
     1441 gtcacgcgcc accatacgca acatccgtca aaacctgttc tgggcttttg cttacaacgt
     1501 ggctttgata cccgtggccg caggcgtgtt gtatccgaag tttggcatac tcttgtcgcc
     1561 gatgtttgct gcaggcgcga tggccttaag ctcggtgttt gtgcttagca atgctttaag
     1621 actgcgccgt ttcaagcctt aagttgatgt gataagcggc ctgtaaacca atatggtgac
     1681 ttacaggccg cttgttttga ttcagggccg agatttctta tgggcattca gcgccaagcg
     1741 gtgcaggtgt gaaagcaatg catgcaaaat aaatatgctg cgaaattgcc caaagctcgt
     1801 aacggtgtat tcggtgtgcc ccaaagtatt gtaaggcttt gagcttatga tgtttatttc
     1861 ataatggcgt ctggaaattt tatgcagttg cacttcaagt atgtctgccc aagcaatgtg
     1921 aaatgtgtgt ggtatttggt ctgcaaaaaa caagcgctgg ccagacatgg cgcgccactc
     1981 caagcctacg gggttgatgc tcagccattc gttgccgtcg tcttgccacg ttaaaccacg
     2041 ggcatcaatg cgcaaccatt caccgccatg ggctgttaca aatagggttt ggaggatttt
     2101 ttctttgcgt gcccaataca cagcaaaggt cagtagggag aaaaacgtgt gcatcccaag
     2161 caaacgcagg ttgtctgctt gccagggcca tttaagttgc aacaagccat gcagcgtgaa
     2221 agtaaaacaa gtcaccaata cggtgttgta aaccagcatt tgccacagtg cttcacggct
     2281 ttttttatca agtcccgcaa atggctcatc tagcagcaag attttttttg ggctaagtaa
     2341 tagccgtgcc
//
LOCUS       DAOMVK010000361         2347 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_13446_length_2347_cov_2.656195, whole genome shotgun sequence.
ACCESSION   DAOMVK010000361 DAOMVK010000000
VERSION     DAOMVK010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13446_length_2347_cov_2.656195"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="PK856_10225"
     CDS             <1..524
                     /locus_tag="PK856_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRL02785.1"
                     /translation="WPISIVPIGWQPNAFPDIRGLKGPFGCLNKARYGISWGAMGAAE
                     ACWFAARQYTLDRKQFGKPLAANQLVQLKLANMQTEIALGLQASLRVGRLMDEDRAAP
                     EMISLIKRNNCGKALDIARVSRDMHGGNGIADEFHVIRHVMNLEAVNTYEGTHDVHAL
                     ILGRAQTGLQAFA"
     gene            599..1789
                     /locus_tag="PK856_10230"
     CDS             599..1789
                     /locus_tag="PK856_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HRL02786.1"
                     /translation="MAQAALAGIKVVDLSRVLAGPSATQCLADLGAEVIKIERPETGD
                     ETREWAPPQFADGTSAYFATVNRNKQSVTVDISTAAGQDIIKKMVADADVLVENYKVG
                     GLKKYGLDYDSLKTLNPRLVYASLTGFGQTGPDARKPGYDYIIQGQSGLMSITGPEDG
                     LPYKVGVAVVDLFAGLNLVIGIQAALLARAQTGVGQHVDVALLDSAIAMLANVGMNHL
                     ATGKVPPRLGNGHPSIVPYQVFATEGAQHLTLACGNDKQFAAVCTVMGQDWIADERFA
                     TNPQRVKNRAELVPLMQAVFMCKERDAWLGLFDAASVPCGPINNIAEAVAMPQVAARE
                     MVVGLNKAEAQMKVLGSPVKLSGTPVQYHSAPPDLGQDTVSVLTSLGYTQEQIEQLQQ
                     QHIV"
     gene            1867..>2347
                     /locus_tag="PK856_10235"
     CDS             1867..>2347
                     /locus_tag="PK856_10235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRL02787.1"
                     /translation="MVENTERESMQFDVVVVGAGPSGLSAAIRLKQLADKAEKEISIC
                     VVEKGSEVGAHILSGAVIDPVSLGELFPNWKELGAPLDCPVTEDAFLFLTADKAKKMP
                     TPPNFQNHGNYIISLGLLTRWLAEQAEGMGIEIYPGFAAAEVLYHADGSVRGIATGDM
                     "
BASE COUNT          596 a          546 c          637 g          568 t
ORIGIN      
        1 attggccaat ttcaattgta ccaattggtt ggcagccaaa cgctttccct gatatccgtg
       61 gcttgaaagg tccgtttggt tgcttgaaca aagcccgtta cggcatttct tggggcgcga
      121 tgggtgcggc tgaagcctgt tggtttgctg cacgtcaata cactttagac cgcaaacaat
      181 ttggtaagcc tttggctgcc aaccaattgg tgcaattgaa attggccaat atgcaaaccg
      241 aaatcgcttt gggtttacag gcttcattgc gcgtgggccg tttgatggat gaagaccgcg
      301 ctgcgccaga aatgatttct ttgatcaagc gcaataactg tggcaaggct ttggacattg
      361 cccgcgtttc tcgtgacatg cacggtggta acggtattgc cgatgaattc cacgtgatcc
      421 gccatgtgat gaacttggaa gcagtgaaca cttacgaagg tactcatgat gtgcatgcat
      481 tgattttggg ccgtgcccaa acaggtttgc aagccttcgc ttaaaaccat ctttgcatga
      541 cttacaggac gctttatgcg tcctgtaaag tatctaaacc gcacaaggag tgttgcacat
      601 ggcacaagct gcattggcag gcatcaaagt cgttgattta tcccgtgtac tcgcaggtcc
      661 aagcgctacg caatgcttgg cagatttggg tgcagaagtc attaaaattg aacgccctga
      721 aacaggggac gaaacccgcg aatgggcacc gccacaattt gccgatggca catcagctta
      781 tttcgccacc gtgaatcgca acaaacaatc ggtcaccgta gacatttcca ctgccgcagg
      841 acaagacatc atcaaaaaga tggtagctga tgcagatgtg ttggtagaga attacaaagt
      901 aggcggcctg aaaaaatacg gcttggacta cgacagttta aaaacattga atccgcgttt
      961 ggtgtatgca tctttaactg gctttggtca aacaggtcca gatgcccgca aaccaggtta
     1021 cgactacatc attcaaggcc aatcaggctt gatgagcatc acaggccccg aagacggctt
     1081 gccctataaa gtgggcgtgg ccgtggtgga tctgtttgca ggtttgaatt tggtcatagg
     1141 tatacaggcc gcattgttgg cgcgtgcgca aacaggtgta ggccagcacg tggatgtggc
     1201 tttattagac agtgccattg ccatgttggc aaacgtgggc atgaatcact tggccacagg
     1261 caaagtgccg cctcgtttgg gtaatggtca tccgagcatc gtgccgtatc aagtgtttgc
     1321 gactgagggg gcgcagcact tgacactggc atgcggcaat gacaagcagt ttgctgccgt
     1381 gtgcaccgta atggggcaag attggatagc cgatgaacgc tttgccacca atccgcaacg
     1441 ggtgaaaaac cgtgccgaat tggtgccatt gatgcaagcg gtgttcatgt gcaaagagcg
     1501 cgatgcttgg ctgggcttgt ttgatgccgc cagtgtgcct tgtggtccca tcaataacat
     1561 tgccgaagcc gtggccatgc cacaagtggc ggcgcgtgaa atggtggtag gtttgaataa
     1621 agctgaagcc caaatgaagg tattgggttc acccgtaaag ctgtcaggca cgcctgttca
     1681 gtatcacagc gcgccacctg atttgggaca agacactgtt tcagtcttaa caagcttggg
     1741 ttatactcaa gagcaaatcg agcaattgca acagcagcac attgtttaac aagctttaat
     1801 caagccagaa gaacttgata tagcatataa acgggatcat cccaaagata ttggaaagcc
     1861 tcgaacatgg tagagaacac agaacgcgaa agcatgcagt ttgatgtggt ggtggtaggc
     1921 gcaggtcctt ctggtctgtc ggcagccatc cgtttgaaac aattggccga caaagctgaa
     1981 aaagaaatca gcatttgtgt ggttgaaaaa ggctcggaag tcggtgcgca cattttgtct
     2041 ggcgcagtga tagacccagt gtctttgggt gaattgttcc ccaattggaa agagttgggc
     2101 gcgcctttgg attgccccgt caccgaagat gcctttttat tcttgaccgc ggacaaggcc
     2161 aaaaaaatgc cgacaccgcc caacttccaa aaccatggca attacatcat cagcttgggt
     2221 ttgctcacgc gctggctggc cgaacaagcc gaaggcatgg gcattgaaat ctaccctggt
     2281 tttgccgccg cagaagtgtt gtaccacgcc gatggctcgg tgcgcggtat cgccaccggt
     2341 gacatgg
//
LOCUS       DAOMVK010000362         2342 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_13483_length_2342_cov_2.647573, whole genome shotgun sequence.
ACCESSION   DAOMVK010000362 DAOMVK010000000
VERSION     DAOMVK010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13483_length_2342_cov_2.647573"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..67)
                     /locus_tag="PK856_10240"
     CDS             complement(<1..67)
                     /locus_tag="PK856_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HRL02788.1"
                     /translation="MLLIPAIDLKDGQCVRLRQGLM"
     gene            complement(335..985)
                     /gene="hisH"
                     /locus_tag="PK856_10245"
     CDS             complement(335..985)
                     /gene="hisH"
                     /locus_tag="PK856_10245"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRL02789.1"
                     /translation="MKIAIVDYGMGNLHSVLKSAQAAQQRAGVEAHISLTDNPDVVYQ
                     ADKVIFPGQGAMPDCMAALQKSGLGEAVVDGLQNKPFFGICVGAQLLFDVSEEGNTAG
                     LGWFSGSVKRFATGLQDAQGDKLKVPHMGWNQVHQTRAHPLFAGVAQDARFYFVHSYY
                     FAPSDTSIVLSSSDYPESFACVVGRDNVFATQFHTEKSHDAGLQLLQNFMLWDGQA"
     gene            complement(1055..1645)
                     /gene="hisB"
                     /locus_tag="PK856_10250"
                     /pseudo
     CDS             complement(1055..1645)
                     /gene="hisB"
                     /locus_tag="PK856_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
     gene            complement(1650..>2342)
                     /locus_tag="PK856_10255"
     CDS             complement(1650..>2342)
                     /locus_tag="PK856_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRL02790.1"
                     /translation="GVPLNEDFTLNVDAVLAAIAEHQPQLIFIAYPNNPTGVPFSVED
                     VNRIIAAAPGLVVIDEAYGAFSSHTFLSQAGSIENVVVLRTLSKIGFAGIRVGYAVGH
                     PNVMHELRKITPPYNMNQLSLTAAKFALQYHQFIDVHINKQKSERERMRTMLKAWSDI
                     QVFPSEGNFITMRVPDAQALYQALLDHNILIKNLHGVHPLLNQCARITVGKPEQNQQV
                     LGVMQKLYGTQA"
BASE COUNT          587 a          604 c          563 g          588 t
ORIGIN      
        1 ccatcaaacc ttgacgcaaa cgcacacatt gaccgtcttt caaatcgatg gcaggaatca
       61 ataacatagt cgtactcgaa tatcacgaaa aagagattaa tcaattgggt catgtttctg
      121 cttagtgcag caacaaaccg tgtcatgttt caggcctgag acctgataaa acagcgcgaa
      181 acattttacc gctattttgc ccgacatgcg tcagccagtt gggcattttt tgtgctgtgt
      241 caccccatca cgacatctaa tcccacattt atgcctgaca atcaagcaca ttgggcttac
      301 aggccgcttt taaatggcac aatctggtct aaacttaagc ttgtccgtcc cacaacatga
      361 agttttgcaa caattgcaaa cccgcatcgt ggcttttttc ggtgtgaaat tgggtggcaa
      421 acacattgtc acgccccacc acacaggcaa aagattctgg gtaatcgctg ctgctcaaca
      481 cgatgctggt atcgctgggc gcaaaataat agctgtgcac aaagtaaaag cgcgcgtctt
      541 gcgccacacc tgcaaacaga gggtgggcac gggtctggtg cacttggttc caacccatgt
      601 gcggcacttt caatttgtcg ccttgcgcgt cttgcaagcc tgttgcaaaa cgcttcaccg
      661 agcctgagaa ccaacccaag cctgcggtat tgccttcttc gctcacgtca aacaacaatt
      721 gcgcgcccac gcaaatgcca aaaaacggtt tgttttgcaa accatcgacc accgcctcgc
      781 ccaaacctga tttttgcaaa gccgccatgc aatcgggcat cgcgccttgg cctgggaaaa
      841 tcactttatc ggcttggtac accacatcgg gattgtcggt aaggctgatg tgcgcttcca
      901 cacctgcgcg ctgctgcgcc gcttgggcag atttcaaaac ggaatgtaaa ttccccatgc
      961 catagtccac aatggcaatt ttcatgatgt tcacgtcctt aaaacaatag aataaaagcg
     1021 gcctgtaaac tgtttacagg ccgcttgatg tggtttaagc ggttaaagtg cctttggttg
     1081 aaggcatgac acccgccatg cgctcatcga attccaccgc cattctgagg gcgcggccaa
     1141 atgctttaaa gatggtttcg gcttggtggt gggcattgat gccacgcaat tggtctatgt
     1201 gtaccgtcat catggcgtgg ttgatcaagc catggaaaaa ctcgctgaac aagtccacat
     1261 caaaacggcc gatggcggcg cgggtgaatt ccacgttgta agtcaaacct gggcggccgg
     1321 acaaatccaa caccacgcgg ctcaaggctt cgtctaaagg cacatagctg tggccataac
     1381 ggcgtatgcc cactttgtcg cctatggcca gctttaaggc ttggcccaag gtgatgccga
     1441 tgtcttcaac ggtgtggtgg tcatcgatgt gcaaatcgcc tttgcaagtg atgtccatgt
     1501 ctatcatgcc gtggcggcag atttgatcta gcatgtgttc caagaaaggg acgccggtat
     1561 caaaacgacc gacgccttta ccatccaaat tgatgctgac actgatttgg gtttcagaag
     1621 tgttgcgatt gacgctggca gtacgcatat taagcttgtg ttccatacag tttctgcatg
     1681 acgcctaaga cttgctgatt ttgctcaggc ttaccaacgg taatgcgcgc acattggttc
     1741 aacagcgggt gcacaccatg caggtttttt atcaaaatat tgtgatcaag cagcgcttga
     1801 tacaaagctt gggcatcggg cacacgcatg gtgatgaaat tgccttcaga tgggaagact
     1861 tgtatgtctg accatgcctt gagcatggtg cgcatgcgct cgcgttccga tttctgttta
     1921 ttaatgtgta catcaataaa ctgatggtat tgtaaagcaa atttggccgc cgtgaggctt
     1981 aattggttca tattgtaagg tggggtgatt tttctcaatt catgcatcac gtttggatgc
     2041 cccaccgcat agcccacacg tataccagca aagcctattt tgctcaaagt gcgcaacacc
     2101 actacgtttt cgatgctgcc cgcttgcgac aaaaacgtgt gcgatgagaa cgcgccatag
     2161 gcttcgtcta tcaccaccaa gccaggtgcg gccgcaatga tgcggttcac atcttctacc
     2221 gagaatggta cgccggtcgg attgttgggg taggcaatga aaatcaactg cggttgatgc
     2281 tcggcaatcg ccgccaacac cgcgtccaca ttcaaagtga aatcctcatt caaaggcacg
     2341 cc
//
LOCUS       DAOMVK010000363         2337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_13518_length_2337_cov_3.036810, whole genome shotgun sequence.
ACCESSION   DAOMVK010000363 DAOMVK010000000
VERSION     DAOMVK010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13518_length_2337_cov_3.036810"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..238)
                     /gene="rpoZ"
                     /locus_tag="PK856_10260"
     CDS             complement(32..238)
                     /gene="rpoZ"
                     /locus_tag="PK856_10260"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HRL02791.1"
                     /translation="MARVTVEDCIPRIPNHFLLTLTAATRARQLAHGATPLVEESGKH
                     KPTVTALREIAAGQIGVELLDRQK"
     gene            complement(252..863)
                     /gene="gmk"
                     /locus_tag="PK856_10265"
     CDS             complement(252..863)
                     /gene="gmk"
                     /locus_tag="PK856_10265"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRL02792.1"
                     /translation="MDKPTHGQIYIISAASGTGKTTIVGQVLKRDPRVRLSISHTTRP
                     VRTGEQHGHDYFFVSVDEFKQLIAKNEFLEYAQVYDNFYGTNANVVRDLQAQGYDVIL
                     EIDIQGATQIRDILPDALSIFIAPPSMETLEQRLVGRGTDSEEVIAKRLGKARFEINQ
                     ARYFDYIVINDDLERACHDVLGIMHMQRLRCPKQLPFIDELLK"
     gene            1047..1643
                     /locus_tag="PK856_10270"
     CDS             1047..1643
                     /locus_tag="PK856_10270"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HRL02793.1"
                     /translation="MLIHPEVFDAEALAQNIRKVPNWPKQGILFHDITPILQDPLHFR
                     LLVDLLVYRYMSQKIDVVAGLDARGFIIGAAVAYQLNVGFVPIRKKGKLPFTTVSENY
                     ALEYGEACVEIHTDAVKPGDRVLLVDDLVATGGTMQAGINLIRRLNAVVVEAVSVLEF
                     SDLPGGERVRQLGVPLFSLCQSEGDMIESKLEENNAKD"
     gene            1646..1807
                     /locus_tag="PK856_10275"
     CDS             1646..1807
                     /locus_tag="PK856_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02794.1"
                     /translation="MIWDALFGTVEGWIAIGVTLLSMALAMGFRLFIRKQINKELAAQ
                     KQATNKPFD"
     gene            complement(2027..>2337)
                     /locus_tag="PK856_10280"
     CDS             complement(2027..>2337)
                     /locus_tag="PK856_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HRL02795.1"
                     /translation="IFIQELRNFKDEQGVSWYDKTSQAFAVFLPVKSVGVMGDGRTYD
                     YVVALRAVITNDFMTAHWAHLPYDLLGTVSNRIINEVRGINRVVYDVSGKPPATIEWE
                     "
BASE COUNT          587 a          478 c          601 g          671 t
ORIGIN      
        1 gattttgcca acgtatttct tcatatctgc cttatttttg acggtctaac aattccacac
       61 cgatttgacc cgctgcaatt tcgcgcaaag cggtcacagt aggtttatgt ttgccacttt
      121 cttcaaccaa tggggtcgcg ccatgagcca attggcgtgc acgggtagcg gcagtcaaag
      181 tcaataaaaa gtgattgggg atgcgaggga tgcaatcttc aacagtaacg cgagccatgt
      241 ttaagacctt tctattttag taattcatca ataaagggta attgttttgg gcagcgcagg
      301 cgctgcatgt gcatgatgcc taatacatca tggcaagccc gttctaaatc atcattgatc
      361 acaatgtaat cgaaataacg ggcttggtta atttcaaatc gggctttgcc caagcgtttg
      421 gcaatgactt cttcgctgtc agtgccacgt cccaccaagc gttgctcaag agtttccatt
      481 gatggcggtg cgatgaaaat actcaaagca tcaggcaaaa tatcgcggat ttgggtggca
      541 ccttggatgt cgatttctaa aatcacatcg taaccttggg cctgtaaatc gcgtactaca
      601 ttggcattgg tgccataaaa attgtcgtac acttgagcgt attctaaaaa ctcgtttttg
      661 gcaatcaact gtttgaattc atcgacgctg acaaagaaat agtcatgacc atgttgttca
      721 cctgtgcgta caggccgagt tgtatgggat atggataaac gcacacgagg gtcacgtttg
      781 agcacttgcc caacaatggt ggttttccct gtgccagaag ctgctgagat gatgtaaatt
      841 tggccgtgtg tgggtttgtc catgggtgtg ttgtccttac caaaatacga ttatgcctga
      901 agccgtgcag tgcagcaatt gcctgcactc ggttaataga cctcatgaga gtgcgatttg
      961 gctgaaatgt tgcagccgca cattataatt tatcttggcg ggttatataa ttacacaatt
     1021 caatttgtgt tccaaaggat ggcaacatgt taattcaccc cgaagtcttt gatgcagaag
     1081 cgttggcgca aaacatccgc aaagtgccta actggccgaa acagggcatt ttgtttcacg
     1141 acatcacccc gattttgcaa gatcccttgc attttcgctt gttggtagac ttattggttt
     1201 accgttatat gtcgcaaaaa attgatgttg tggcaggctt ggatgcgcgt ggtttcatca
     1261 tcggtgcggc tgtggcctac caattgaatg tgggctttgt gccgattcgt aaaaaaggca
     1321 aattgccctt caccacggtt tctgaaaact atgctctgga atacggtgag gcttgtgtgg
     1381 aaatccatac cgatgcggtg aagcccggcg atcgtgtgtt gttggtagat gatttggtgg
     1441 ccacgggtgg caccatgcaa gcggggatta atttgatacg ccgtttgaat gcggtggtgg
     1501 tggaagcggt ttcggttttg gaattcagtg atttaccagg cggcgaacgc gtacgtcaat
     1561 tgggcgtgcc tttgtttagc ttgtgtcaaa gcgagggcga catgattgag tctaaactag
     1621 aggaaaataa cgctaaggat tgattatgat ttgggatgca ttgtttggca ctgtcgaggg
     1681 ttggattgcg attggggtga cgctgctgag tatggccttg gccatgggat ttcggttgtt
     1741 tatccgcaag caaatcaaca aggaattggc agcgcagaaa caagcgacta acaaaccatt
     1801 tgattgatgt cttgtaatgt aaacggcctg taaacctttg agggcttaca ggccgtttgt
     1861 tagtagcttg tagtattcga ttggttgtca gtattgagtt taggttcgca ttaaactaat
     1921 aggattgtca tgagtgatta aattcacata gatcaaaaaa agtcccacaa gtgcagggct
     1981 ctattgaaat tttgaaatct tatgaactag gtgtaatgag ttgcaactat tcccactcta
     2041 tcgtggctgg tggcttacca gatacgtcat acaccacgcg gttgatgccg cgaacttcgt
     2101 tgatgatgcg attggacacg gtgccgagca agtcgtatgg caagtgtgcc cagtgtgcgg
     2161 tcatgaagtc gttggtaatc acggcgcgca gtgccaccac gtagtcatag gtgcggccat
     2221 cgcccatcac gcccactgat ttcacaggca agaacacagc aaaggcttgg ctggttttgt
     2281 cataccaaga cacgccttgt tcgtctttga aattgcgcaa ttcttggatg aaaatat
//
LOCUS       DAOMVK010000364         2317 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_13654_length_2317_cov_3.023873, whole genome shotgun sequence.
ACCESSION   DAOMVK010000364 DAOMVK010000000
VERSION     DAOMVK010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13654_length_2317_cov_3.023873"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="PK856_10285"
     CDS             <1..605
                     /locus_tag="PK856_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003785914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRL02796.1"
                     /translation="TENLMGYDNGNFVSMQGKRVVVLGGGDTAMDCVRTSIRQEAAEV
                     ICAYRRDADNMPGSRKEFQNAKEEGVQFTFNAQPVAIETDAAGKVTGIQLVKTQMGAP
                     DERGRRRAEVVAGSEEVLACDAVIIAFGFAPHAMPWLEAVGVGVDSRGRIAQEGVLKQ
                     QTANAKIFAGGDVTRGSDLVVTAVAEGRDAAQSILEYLDI"
     gene            complement(948..1343)
                     /locus_tag="PK856_10290"
     CDS             complement(948..1343)
                     /locus_tag="PK856_10290"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRL02797.1"
                     /translation="MIDIATLAKQTGTPAHTLRYYETRGLIAPVSRSGLKRVFADDVV
                     LKLQLIQLGQQVGMSLDDINAAFGLNADMAVKRSALAQQAATVAMQIRQLQTVQTLLQ
                     HLLACPHEQHLDCPEFLALLQTGLPVSDT"
     gene            1441..1914
                     /locus_tag="PK856_10295"
     CDS             1441..1914
                     /locus_tag="PK856_10295"
                     /inference="COORDINATES: protein motif:HMM:NF022606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2938 family protein"
                     /protein_id="HRL02798.1"
                     /translation="MHTTHWSTLIWFGIFSTLGMDVLLYLRHKWLHQPWFDYRVLGLA
                     LLKTTLPQVVKHTHANTAASLLGWGLHYVIGVLLAVSAVYLATILGFNTLSLAFCLLY
                     GMATVILPFCSMQPLLGLGFAASKTAQPWRNRGKSLFTHALFGLSLYLTALCFWP"
     assembly_gap    1975..1984
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1985..>2317)
                     /locus_tag="PK856_10300"
     CDS             complement(<1985..>2317)
                     /locus_tag="PK856_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="short chain dehydrogenase"
                     /protein_id="HRL02799.1"
                     /translation="GLQDKFLGQVRLALIGQEFVHEGGSITLTTGITGHEPIAAGANA
                     TAVNVGIEGFVQAAALELPNRIRINAVSPNVLTEALPDFAPYFPGFGSVSGAEAARAY
                     QRSIEGIQT"
BASE COUNT          547 a          613 c          568 g          579 t
ORIGIN      
        1 ataccgaaaa cctgatgggc tacgacaacg gcaactttgt gtccatgcaa ggcaaacgcg
       61 tggtggtgtt gggtggtggt gacactgcca tggactgtgt gcgcacatcc atccgccaag
      121 aagccgccga agtcatctgc gcttaccgcc gcgatgccga caatatgcca ggctcgcgca
      181 aagagtttca aaatgccaaa gaagaaggcg tgcaattcac attcaatgcc caacccgtgg
      241 ccattgaaac cgatgccgca ggcaaagtca caggcatcca attggtgaaa acccaaatgg
      301 gcgcgcccga tgagcgtggc cgtcgccgtg ctgaagtggt ggcaggctct gaagaagtgt
      361 tggcgtgtga cgcggtcatc atcgccttcg gtttcgcccc tcacgccatg ccatggttag
      421 aagcagtcgg cgtgggcgtg gacagccgcg gccgcatcgc ccaagaaggc gtgttgaaac
      481 agcaaaccgc caatgccaaa atcttcgcag gtggcgacgt cacccgcggt tctgacttgg
      541 tggtcaccgc cgtggccgaa ggccgcgacg ccgcccaaag cattttggaa tacttggaca
      601 tttaaatctg tattgctgac tcgatgacaa ataagcggcc tgtaaatgtt ttaaacacgt
      661 ttacaggccg cttgttttat ttatcttatt ctatgccatg tgaccgtata gccactgtaa
      721 gaaaatcacc acagctgtgc gtaaatccac tgtaaagtga ttgcctacct gcccgtctgt
      781 ttcttcatga cttgattgaa taacatcttg gcaaaacacc tactcacgaa atcaaagcag
      841 ttccacactt cgttctagca tcattaccct caccgtcatg cgccatatca agtgtatcaa
      901 gacacctttt ttgcatttgt atgctgatgg tcgttctaag gtgtccgcta tgtgtcagac
      961 acaggcaagc ctgtttgcaa caaagccaaa aattctgggc aatccaaatg ctgttcatgt
     1021 ggacatgcaa gcagatgttg caataaggtt tgaacggtct gcaattgtcg aatctgcatc
     1081 gccaccgtcg ccgcctgttg cgccaaagca ctgcgtttca ccgccatatc ggcattcagg
     1141 ccaaaggccg cattgatgtc gtccaaagac atgcccactt gctgccccaa ttgtatcaat
     1201 tgcagcttca acaccacatc atcggcaaac acccgcttta agccactgcg gctcacgggc
     1261 gcaatcaggc cacgcgtttc gtaatacctg agcgtgtgcg caggggtgcc tgtttgcttg
     1321 gccaaggtgg cgatgtctat catgttgttc tctgtgagag tgtttgcggg tgtcaaagcc
     1381 tattgactta aagttggctt caagttgtac agtctaacac acacaatatg gagacaacac
     1441 atgcacacta cccactggag cacattgata tggtttggga ttttcagcac cttaggaatg
     1501 gatgtcttgc tatatctgcg ccacaaatgg ctgcatcagc cttggtttga ttaccgtgtt
     1561 ttagggctgg ctttgcttaa aaccactctg cctcaggttg taaaacacac ccatgccaat
     1621 accgcagcca gcctattggg ttggggttta cactatgtga taggtgtatt actggccgta
     1681 tccgccgttt atttggcaac gattttgggt ttcaacacct tgagcttggc tttttgtttg
     1741 ctctatggca tggcaacagt tattctgcca ttttgcagca tgcaaccttt acttggcttg
     1801 ggatttgccg ccagcaaaac cgcccagcct tggcgcaacc gtggcaaatc actgttcaca
     1861 cacgcgctgt ttggactgag cctgtatttg acggcactgt gtttctggcc ttaaagatca
     1921 cacaagcggc ctgtaagtgt tttacaggcc gcttgtttat tacatcatag cacgnnnnnn
     1981 nnnngtttgt atgccctcaa tactgcgttg gtaggcacgc gcagcctcgg cgccgctcac
     2041 actgccaaaa ccagggaaat aaggtgcaaa atcgggcaag gcttcggtca acacattcgg
     2101 gctgacggcg ttgatgcgga tgcgattggg caattccaag gccgctgctt gcacaaatcc
     2161 ttctatgcca acattcaccg ccgtggcatt ggcgcctgct gcaatcggct catgcccagt
     2221 aatgcctgtg gtcaaagtga tagagccacc ttcatgcaca aattcttggc cgattaaggc
     2281 caaacgcact tggcctaaga acttgtcttg caaaccc
//
LOCUS       DAOMVK010000365         2305 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_13733_length_2305_cov_2.356444, whole genome shotgun sequence.
ACCESSION   DAOMVK010000365 DAOMVK010000000
VERSION     DAOMVK010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13733_length_2305_cov_2.356444"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1220)
                     /locus_tag="PK856_10305"
     CDS             complement(<1..1220)
                     /locus_tag="PK856_10305"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRL02800.1"
                     /translation="MSPLHTPLRPIALLLLGLTGWAHATDAETTIEADTELPSITVTA
                     SQNTGQVGKEQINVRQADSIRDVLRDIPGVYVGGTNNMNQQIQVRGIGESGLNISIDG
                     ARQLGTVFHHNGRTIIDPDLLKRVNVDVGSRSVSSGMGALGGAVQFTTISAGDMLQDG
                     EAFGGKVKSSYATNNQQWQNSLMLFGKPMDQLELLGYVNYKNMNLGESGDNRPLGGDG
                     KEVDYLVKAAWQFSPSQKVTLSAERNDLSGLYPARLEFPHNDFYQKPGLQYNPLIHQT
                     YRRDTQTIAYTYQPSDNDWFDVEANVYRTRVYLDNAKDAPVPGLDWLAKLQIETVGGT
                     LKNTSHFDTGAANHRLRVGYEFYDTDNEKQDLNHSRTTQGESGRLHSLYVEDKIAIGN
                     FSLTPGMRYDHYTL"
     gene            1434..>2305
                     /locus_tag="PK856_10310"
     CDS             1434..>2305
                     /locus_tag="PK856_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02801.1"
                     /translation="MVNLYQILGVSADADAATIAFAISESRLQGDINAQVLDKAEEWL
                     LQAEVRAKYDAQLKQEDAAFFSQTSHASTQTDFPQSNQASTAPVFEASNAASTASNNT
                     ERVHAPQNHAQTERPKFKTAQVKTNSKQRINIKRDGKKSNSRGCLVAFLIVSVLGVIL
                     LGVISWWGYSLFQDVKKTLNEYEIEDMQTTPGWHVGDGGHVVYAVAQNQPNMVLMLVG
                     EDSQPMLANRDSSQAINCHKDQDLLCSIQVQFDQDPARTYEAGQLGNQILVSDDADKA
                     EMVLRMQDADKLSI"
BASE COUNT          597 a          552 c          592 g          564 t
ORIGIN      
        1 tccaaggtgt agtggtcgta gcgcatgcca ggggtgaggc tgaaattgcc aatggcgatt
       61 ttgtcttcaa catacaaaga atgcaaacgg ccgctttcgc cttgggtggt gcggctgtga
      121 ttcaagtctt gtttctcatt gtcggtatcg taaaactcat agccaacgcg caaacggtgg
      181 ttggcagcac ccgtatcaaa atgcgacgtg tttttcagcg taccgcccac ggtttcaatt
      241 tgcagctttg ccagccaatc cagcccaggc acaggcgcgt ctttggcatt gtccaaatac
      301 acacgggtgc ggtagacatt ggcttcaaca tcaaaccaat cattgtcact cggctggtag
      361 gtgtaagcga tggtttgggt gtcgcggcgg taggtctgat ggattaatgg gttgtattgc
      421 aagcctggct tttggtagaa atcattgtga ggaaactcca aacgggcagg gtacaggccg
      481 cttaaatcat tgcgctcggc cgacagtgtg actttttggc tggggctgaa ttgccacgcg
      541 gctttcacca agtaatccac ttctttgcca tcgccaccca agggacgatt gtcgcctgac
      601 tcgcccaaat tcatgttttt gtaattgaca tagcccaata attccagttg gtccattggt
      661 ttgccaaaca gcatcaatga gttttgccat tgctggttgt tggtggcgta actggatttg
      721 actttgccac caaatgcttc gccatcttgc aacatgtcac ctgcgctgat ggtggtgaat
      781 tgcaccgcac cacccaaggc gcccatgcca ctgctgaccg agcgactgcc cacatccacg
      841 ttcacgcgct tgagtaagtc ggggtcaatg atggtgcgac cattgtggtg aaacaccgtg
      901 cccaattggc gcgcgccgtc tatgctgatg ttcaggccgc tttcacctat gccacgcacc
      961 tgtatttgtt ggttcatatt gttggtgccg cccacataca cgccaggaat gtcgcgcaac
     1021 acatcgcgta tgctgtcggc ttggcgcacg ttgatttgct ccttgcccac ttgacccgtg
     1081 ttttggctgg cggtgacagt gatgctaggc agttcggtat cggcttcaat cgtcgtttcg
     1141 gcatcggtgg catgggccca gccagtcaag ccaagtagca ataaggcgat ggggcgcaga
     1201 ggtgtgtgta atggggacat aggaacgggt attcagacga aaatcataaa gaaaagtatt
     1261 ctaatacata tgataataat tttcattcta aaaatataag gcttgcgatt tacttacagg
     1321 ccgcatggaa gaaaaaccaa acacaccacc catcctgctg aaagttagac tgcctattca
     1381 aatggcgtaa gtattcagta tactgagggc tcaattcaaa caaggggaaa cagatggtca
     1441 atttgtacca aatcttgggc gtgagtgccg atgccgatgc cgccaccatt gcatttgcca
     1501 tcagcgaaag ccgtttacaa ggtgacatca atgcccaagt tttggataaa gccgaggaat
     1561 ggctgttgca agcagaggtg cgggccaaat acgatgctca gttaaaacaa gaagatgccg
     1621 cattcttttc tcaaaccagc catgccagca cacaaacaga ttttccccaa tcgaatcaag
     1681 ccagcactgc gcctgtgttt gaagctagca atgccgcatc aactgcgtca aacaacacag
     1741 agcgcgtgca cgccccacaa aaccatgctc aaacagagcg ccctaaattc aaaactgctc
     1801 aagtaaaaac caacagcaaa cagcgcatca atatcaaacg agatggcaaa aaatccaaca
     1861 gccgtggctg tttggtggcg tttttaatcg tatcggtttt gggcgtgatc ttgttgggcg
     1921 tgatttcgtg gtggggctat tctctgttcc aagacgtgaa gaaaacattg aatgagtatg
     1981 aaattgaaga catgcaaacc acaccaggtt ggcatgtggg agacggtggg catgtggtgt
     2041 atgcggtggc gcaaaatcag cccaatatgg tgttgatgct cgtgggcgaa gattcacaac
     2101 cgatgttggc caaccgtgac agctcgcaag cgatcaattg tcacaaagac caagacttgc
     2161 tttgcagtat ccaagtgcaa ttcgatcaag atcctgcacg cacttatgaa gcaggccaat
     2221 tgggcaatca aatcttggtg agcgatgatg ccgataaagc agaaatggtg ttgcgcatgc
     2281 aagatgccga caaattgagc attaa
//
LOCUS       DAOMVK010000366         2299 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_13772_length_2299_cov_3.215686, whole genome shotgun sequence.
ACCESSION   DAOMVK010000366 DAOMVK010000000
VERSION     DAOMVK010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13772_length_2299_cov_3.215686"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..80)
                     /locus_tag="PK856_10315"
     tRNA            complement(5..80)
                     /locus_tag="PK856_10315"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45..47),aa:Asn,seq:gtt)
     gene            complement(167..847)
                     /gene="rpe"
                     /locus_tag="PK856_10320"
     CDS             complement(167..847)
                     /gene="rpe"
                     /locus_tag="PK856_10320"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRL02802.1"
                     /translation="MNYRISPSILSADFARLGEEVKNVIAAGADLIHFDVMDNHYVPN
                     LTFGPMICEAIKPYSTVPMDVHLMVEPVDDLIQSFAKAGANIITFHPDATRHVDRSLS
                     LIQAQGCQAGLVLNPATPLHVLDYVLDKIDMVLLMSVNPGFGGQSFIPSTLDKTRALR
                     LKLDEYAKQSGRHIAIEVDGGIKVNNIAEVAAAGADTFVAGSAIFGQDDYQAVIAAMR
                     AELAKLNS"
     gene            1089..1442
                     /locus_tag="PK856_10325"
     CDS             1089..1442
                     /locus_tag="PK856_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02803.1"
                     /translation="MGSLLGIQPAQAAGMNCDVRQLSLSQAQQNQLRLLRNQYRILRD
                     EQTRKKPSATASRRYLVQILMQDDFNENAARQYVSQKYQYNAELDMQDLRMQHYFFKV
                     LTPQQKQQWIRYCQQ"
     gene            complement(1559..2089)
                     /gene="ppa"
                     /locus_tag="PK856_10330"
     CDS             complement(1559..2089)
                     /gene="ppa"
                     /locus_tag="PK856_10330"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HRL02804.1"
                     /translation="MNLDAIKPAKSIPDDFNVVIEITANSSSPIKYEFDKESGALCVD
                     RFMGTSMMYPANYGFVPNTLSGDGDPCDVLVVTPFPLPSGVLVRCRALGVLKMEDDGG
                     MDAKIIAVPVEKLCQMYKDIKTLEDLPQLLRDQIQHFFEHYKDLEAGKWVKIQGWEGI
                     EGATAELEAGVKNYVK"
BASE COUNT          613 a          538 c          560 g          588 t
ORIGIN      
        1 aatttggctc cccgacctgg gctcgaacca gggacctgcg gattaacagt ccgtcgctct
       61 accgactgag ctatcaggga atggagtgca ttatagcggc tgaaatatta ctgtcaagcg
      121 ctgtaaacca atgcagacaa tgttttgcgg cttgctgctg attttattaa gaatttaatt
      181 ttgccaattc tgcacgcatg gcggcgatga cggcttggta atcgtcttgg ccaaagatgg
      241 cagagcctgc cacaaaagta tcggcacctg cggcggccac ttcagcgatg ttgttcactt
      301 tgatgccacc gtcgacttcg atggcaatgt gacggcctga ttgtttggca tactcgtcga
      361 gtttcaaacg caaggcacgg gttttgtcca aagtgctggg gataaagctt tggccaccga
      421 aaccaggatt caccgacatc aacaagacca tgtcgatttt gtccaaaaca taatccagca
      481 cgtgcaaagg tgtggcagga ttaagcacca agcctgcttg gcagccttgt gcttgaatca
      541 aactcaggct acggtcaaca tggcgggtgg catctgggtg gaaggtgata atgttggcac
      601 ccgctttggc aaaagactgt atcaagtcat cgacaggctc taccatcaga tgcacatcca
      661 tcggcacggt ggaataaggt ttaatggctt cacatatcat cgggccgaag gttaggttgg
      721 gcacatagtg gttgtccatc acatcgaaat gaatcaagtc agcaccagcg gcaatgacgt
      781 ttttcacttc ctcacccaag cgggcgaagt cggcagacaa gatactgggg gagatgcggt
      841 agttcatgtg ggtgtcgttt ttattaaaat ggatattgta gcgattttgc tgcaatgcag
      901 ccatgtcatt tgggtctata aatcagattc ttagctctga aagccgccta tttacacaga
      961 tttacgggca gatggttcag tttcacaccg tcactgctac aatgcagtca ttctgtattt
     1021 tggccataca catgggaata aagcgtccaa tcaccaataa caaatcatta tggatgttcg
     1081 gtctcttaat gggcagtttg ttaggtattc aacccgcgca ggccgcgggc atgaattgtg
     1141 atgtcagaca attgtcactc agtcaggcac agcaaaatca gttgcgcttg ctgcgcaacc
     1201 aataccgtat tctgcgtgat gagcaaacgc gcaaaaaacc atcggccaca gcctcacgcc
     1261 gttatttggt gcagattttg atgcaagacg atttcaatga aaatgccgcg cgccaatatg
     1321 tgagccagaa ataccaatac aatgccgagc tagacatgca agacttgcgc atgcagcatt
     1381 atttcttcaa ggtgttaaca ccgcagcaaa aacagcagtg gatacgttat tgccaacagt
     1441 aaatgatgtt acaggccgct taggaaaatc aagcggcctg taaacatttg gcgcttgtac
     1501 tcagacatga gccatgaaaa aagcccactt taaagtgggc ttttttgtgc acgaaaactt
     1561 acttcacgta gtttttcacg ccagcttcca attcagcagt agcgccttca atgccttccc
     1621 agccttggat tttcacccat ttgccagctt ccaaatcttt gtagtgttca aagaaatgtt
     1681 ggatttggtc gcgcaacaat tgtggcaagt cttccaatgt tttgatgtct ttgtacattt
     1741 ggcacaattt ttccacaggc acggcgatga ttttcgcatc catgccgccg tcgtcttcca
     1801 ttttcaacac gcccaaagcg cggcaacgca ccaatacgcc agatggcaat gggaatggcg
     1861 ttaccaccaa tacatcgcaa gggtcgccgt caccagacaa ggtgttaggc acgaaaccgt
     1921 agttggcagg gtacatcata gacgtgccca tgaaacggtc tacacacaat gcgccgcttt
     1981 ctttgtcaaa ttcgtatttg atgggtgaag aagagttggc ggtaatttca atcaccacgt
     2041 taaagtcatc agggatgctt ttagcgggtt tgatggcatc aagattcata gtgtcctcaa
     2101 gtattgttga cggataaacc gcgtcattat agcagtatca agcccgctta cgcgtaaaaa
     2161 accgcattga tgcacatcaa aacaaacctg caggcagcgt ggtcaattgg ctggtattgg
     2221 gcaaggtgtg gtcactgcga tgcgcaagca aatcgggatg gtcgtcgcag taaatgatgt
     2281 caaacgccaa acggttttg
//
LOCUS       DAOMVK010000367         2297 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_13789_length_2297_cov_3.655665, whole genome shotgun sequence.
ACCESSION   DAOMVK010000367 DAOMVK010000000
VERSION     DAOMVK010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13789_length_2297_cov_3.655665"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..>289)
                     /locus_tag="PK856_10335"
     CDS             complement(2..>289)
                     /locus_tag="PK856_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HRL02805.1"
                     /translation="AELADILVDKVAGMNKNDAKDIVELFFEEIRQALESGEEVKISG
                     FGNFQLRDKPQRPGRNPKTGEEVPISARRVVTFHASQKLKGMVEHHYDKQR"
     assembly_gap    290..299
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(384..>2297)
                     /gene="pheT"
                     /locus_tag="PK856_10340"
     CDS             complement(384..>2297)
                     /gene="pheT"
                     /locus_tag="PK856_10340"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRL02806.1"
                     /translation="LALNDALFTLKITPNRADCLGIKGIAREVVALTQCEHNTFTIPT
                     VPVSTGCIQTVRIDAPADCGRFLARVIEGVDAKATTPSWMVQRLQRSGIRSISALVDI
                     GNYVMLELGQPMHIFDADKVTGSIIVRRAQDGEKLLCLNDKEVVLAADTLVVADEAGA
                     LSMAGLMGGAASAVSDETVNVVLEAAWFQPDTLANKSRQYGFGSDSSYRYERGVDYRL
                     QADAIERAAFLVHSICGGKVGAVTEALGDLPVANKVNVRTGRINKVLGIEVPVDTVAT
                     ILTHLGLNPVATADGFEVTAPSFRFDIEQEVDIIEEVGRVYGYDKIPDDYTSGRLHML
                     KRPENRKTRFSVYNALAARGYREIVSYAFVDETWEQDFAANSDPIRLQNPLASHMSVM
                     RSTLIGGLIKTLQHNLNYKQNRVRLFEIARVFKKDGQGAYQQNERVGGLAYGYAVPEQ
                     WGEASRLVDFFDVKGDLEQLIHRSLSVQYQAFEHPAFHPGRCAQLLIDGVAVGVLGQL
                     HPQWVQKYDLPQAPVLFEVDLSVLLQSQAVTYQAVSKLPPVRRDLAFVIADAVKSADL
                     LNTLRAAAGPLVKSVDLFDVYTGVGIEPGHKSMAVKVMLQAASETLTDAVVDEEMQSL
                     IKVAQETGATLRA"
BASE COUNT          594 a          613 c          508 g          572 t
ORIGIN      
        1 attatctttg cttgtcataa tgatgttcca ccatgccttt taatttttgg cttgcgtgga
       61 aagtgacaac gcgacgggcg ctgatgggta cttcttcgcc agttttggga ttgcgacctg
      121 ggcgttgagg tttgtcacgt aattggaagt tgccaaaacc tgagattttg acttcttcgc
      181 cactttccaa agcctgacga atttcttcaa aaaacaactc gacaatgtct ttggcatcat
      241 ttttattcat tccagccact ttgtcgacca aaatatctgc caattcggcn nnnnnnnnnt
      301 gttaacgtca tgttcctact acctttatac ttaatgggcg ctattatcag caaaaaaatc
      361 tatttattca ataccttgac aaatcaagcg cgaagtgtag cacccgtttc ttgggccact
      421 ttaatcaaac tttgcatttc ttcatccacc acggcatcgg ttaaggtctc agatgcggcc
      481 tgtaacatca cttttaccgc catggatttg tgacctggct caatgcccac acctgtgtac
      541 acatcaaaca agtccaccga tttcaccaat gggccagcag cggcacgcaa ggtgttcagc
      601 aagtcggcag atttcaccgc atcggcaatc acaaatgcca aatcacgacg cacaggtggc
      661 aatttggaaa cggcttggta agtcaccgct tggctttgca acaacacact caagtccact
      721 tcaaacaaca caggggcttg tggcaaatcg tatttttgta cccattgcgg atgcaattga
      781 cccaatacac caaccgccac accatcaatc aacaactgcg cacagcggcc tggatggaaa
      841 gctgggtgtt caaacgcttg gtattgcacc gacaaactgc ggtgtatcaa ttgttccaaa
      901 tcgcctttga catcaaagaa gtccaccaaa cggctggctt cgccccattg ctcgggcacg
      961 gcataaccat aggccaaacc gcccacacgc tcattttgct ggtaagcacc ttggccatct
     1021 tttttgaaca cgcgtgcgat ttcaaacaag cgcacgcggt tttgtttgta attcaaattg
     1081 tgttgcaagg ttttgattaa gccgccaatt aaggtagagc gcatcacgct catgtggctg
     1141 gccaatgggt tttgcaaacg aatcggatcg gagttggccg caaagtcttg ctcccaagtt
     1201 tcatccacaa acgcatagct gacaatttcg cggtagccgc gtgctgccaa ggcattgtaa
     1261 acagagaaac gggttttgcg gttttcaggc cgcttgagca tgtgtaagcg gcctgaagtg
     1321 taatcatcag gaatcttgtc gtaaccatac acgcggccaa cttcttcaat gatgtccact
     1381 tcttgctcga tgtcgaaacg gaagcttggt gcagtcactt caaagccatc ggcagtggcc
     1441 acaggattca aacccaagtg cgtcaaaatg gtggcaacag tgtccacagg cacttctatg
     1501 cccaacactt tattgatgcg gcctgtacgt acattgactt tattggcaac gggcaaatca
     1561 cccaaagcct cagtcaccgc accgacttta ccgccacaaa tgctgtgtac caagaatgct
     1621 gcgcgttcaa tcgcatcggc ctgtaaacgg taatcgacac cgcgctcata gcggtaagaa
     1681 gaatccgaac caaaaccata ttggcgtgat ttattggcaa gcgtatcggg ctggaaccat
     1741 gcagcttcta acacgacatt cactgtttca tcagacacgg cactggctgc tccacccatc
     1801 aaacctgcca tgctcagcgc acctgcttcg tctgccacca ccaaagtatc ggcagccaat
     1861 accacttctt tgtcgttcaa acacagcaat ttttcgccat cttgagcgcg gcgcacgata
     1921 atcgaacctg tgactttgtc ggcatcgaat atgtgcatgg gttgacccaa ttccaacatc
     1981 acgtaattgc caatgtccac caaagccgaa atcgagcgta tgcctgaacg ttgcaagcgt
     2041 tgcaccatcc agctaggcgt tgtcgcttta gcatccacgc cttcaatcac gcgtgccaag
     2101 aaacgaccac aatcggcagg tgcatcgatg cgcacggttt ggatgcagcc tgtagacaca
     2161 ggaacggtag gaatggtgaa tgtgttgtgt tcacactggg tcaaagccac cacttcgcgc
     2221 gcgatgcctt tgatgcccaa acaatcggca cggttagggg tgattttcaa ggtaaacaag
     2281 gcatcgttca aagccaa
//
LOCUS       DAOMVK010000368         2292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_13835_length_2292_cov_2.108181, whole genome shotgun sequence.
ACCESSION   DAOMVK010000368 DAOMVK010000000
VERSION     DAOMVK010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13835_length_2292_cov_2.108181"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PK856_10345"
     CDS             complement(<1..406)
                     /locus_tag="PK856_10345"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRL02807.1"
                     /translation="MKRVVVTGMSGITSLGEDTETIMQHMLAGESGIRYMPDWERYAD
                     LRTRLGGPVLHFTEPAHFTRKVKRGMGRVALMATIVAERALDDAGLLGHEIISNGQTG
                     VAFGSSSGNVEAIAEFGAMVLDNDVKRVNATSY"
     gene            complement(406..1134)
                     /gene="fabG"
                     /locus_tag="PK856_10350"
     CDS             complement(406..1134)
                     /gene="fabG"
                     /locus_tag="PK856_10350"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HRL02808.1"
                     /translation="MTRHIFISGSSRGLGKAIALRLAQDDWELTLHGRTASAALMDTV
                     AEVAAISRHPVHHLCFDVADEAASRLVLEQHLIDHPAFYAVVLNAGISSDGAFPALSS
                     QDWRSVIDTSLNGFYHVLQPLTMPMVRLKQGGRIVTMASVSGITGNRGQVNYSAAKGG
                     IIAASKALALELAKRRITVNCVAPGLIETDMVNEEVIAHAMPQIPLQRMGQADEVAAL
                     VNFLLSDDAAYITRQVISINGGMV"
     gene            complement(1131..1571)
                     /locus_tag="PK856_10355"
     CDS             complement(1131..1571)
                     /locus_tag="PK856_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02809.1"
                     /translation="MHDLESLMVHRAPMLLLDELLEYGDDYAVSEVEIQPEMLFMEAQ
                     GLPSWVGIELMAQTVSLLAGMRGKLSGQAPQIGFLLGTRRYHAPMSHFPVGSRLRIHV
                     QESFVDGNKLGQYDCRIDSEQGSIEAKLNVYQPSDAHSLIKEDI"
     gene            complement(1564..>2292)
                     /locus_tag="PK856_10360"
     CDS             complement(1564..>2292)
                     /locus_tag="PK856_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRL02810.1"
                     /translation="VSTACSSAAKAILAGQRLLQADVCDAVLVGGVDTLCSLTFNGFD
                     CLESLSAERCRPFAQDRDGINIGEAGALFLLHRERENVVLRGGGESSDAWHISAPHPE
                     GKGAAQAMQNALSSTGLQAADIDYINVHGTGTPQNDAMEALAIRQVFAKHFPWVSSSK
                     HQTGHCLGAAGAIEAAIACAVLESHETWLPWQQGHAALNPDFMDLSFVKPDSEFTGRI
                     KRIMSNSFAFAGSNASLIFENTHA"
BASE COUNT          537 a          635 c          597 g          523 t
ORIGIN      
        1 tgtaagacgt ggcattgact cttttgacgt cgttgtccaa taccatggcg ccaaattcag
       61 caatggcttc aacattgccc gaagatgacc caaaggccac gcctgtttgg ccattgctga
      121 taatctcgtg tcccaataaa cccgcatcat ccaaggcccg ctctgccaca atggtggcca
      181 ttagggcgac acggcccata ccacgcttca ctttgcgggt gaagtgtgcg ggctcagtga
      241 aatggagtac agggcctccc aagcgggtac gcaaatcggc atagcgttcc cagtcgggca
      301 tatagcgtat gccgctttcg cccgccagca tgtgctgcat gatggtttca gtgtcttcac
      361 ccaaagaggt gatgccggac atgccagtga cgaccactcg tttcatcaga ccatcccccc
      421 attaattgaa atcacttggc gggtgatata cgccgcgtca tcgctgagta aaaaattcac
      481 caaggccgcc acttcatctg cttgccccat gcgctgcaag ggaatttgcg gcatggcgtg
      541 ggcaatgact tcttcgttca ccatatcggt ttctatcaag ccaggcgcca cacaattgac
      601 ggtaatgcgg cgcttggcca attctaaagc caaggctttg ctggcggcaa taatgccgcc
      661 tttggcagcg ctgtaattca cttgtccacg gttgcccgta atgccagaaa ccgatgccat
      721 ggtcacaata cggccgcctt gtttcaggcg caccatgggc atggttaaag gctgcaacac
      781 atggtaaaac ccattgagcg aggtatcgat gacgctgcgc caatcttggc tgctcaatgc
      841 gggaaaagcg ccatcgctgc tgatgcctgc gttgagtacc acggcataaa aagcagggtg
      901 gtcaatcagg tgttgttcca acacgaggcg gctggctgcc tcatcggcta catcaaaaca
      961 cagatggtgc acagggtggc ggctgatggc ggccacttcg gcaaccgtgt ccataagcgc
     1021 ggcagatgcc gtgcggccgt gtagggtcaa ttcccagtcg tcttgtgcca gacgcaaggc
     1081 gatggccttg cccaagccgc gactggagcc gctgatgaag atgtggcggg tcaaatgtct
     1141 tcctttatca aactgtgtgc atcgctgggt tggtagacat tgagtttggc ttctatgctg
     1201 ccttgctcgc tgtcgatgcg acagtcgtat tgtcccagtt tattgccatc aacaaaactc
     1261 tcttgtacat gaatgcgcaa gcggctgccc acaggaaagt gactcatggg ggcatggtag
     1321 cggcgtgtac ccaataaaaa accaatttgc ggtgcttggc cactgagctt gccacgcatg
     1381 cccgcaagca ggctcacggt ttgcgccatc aattctatcc ctacccaact gggcaggcct
     1441 tgcgcttcca taaacagcat ttcaggttga atttcaactt cgctgacggc atagtcatcg
     1501 ccgtattcca gtaactcatc caataacaac atcggtgcac ggtgcaccat caagctttct
     1561 aaatcatgca tgggtgttct caaaaatcaa actggcattg ctgccagcaa aggcaaacga
     1621 attgctcatg atgcgcttga tgcggcctgt aaactcactg tcaggcttaa caaacgacaa
     1681 atccataaaa tctgggttta atgcagcgtg gccttgctgc caaggcagcc aagtttcgtg
     1741 gctttccaat actgcgcagg caatggcggc ttcgatggcg ccagcagcgc ccaagcaatg
     1801 gcctgtttgg tgtttgcttg agctgaccca agggaagtgt ttcgcaaaca cttggcgtat
     1861 ggccaaggct tccatggcat cgttttgcgg cgtgcccgtg ccatgcacat tgatgtaatc
     1921 aatgtcagcg gcctgtaaac ccgtgctgga aagggcattt tgcatcgctt gcgccgcgcc
     1981 tttaccttct ggatgtgggg cggaaatgtg ccaagcatca gaagattcgc caccaccacg
     2041 caaaaccacg ttttcacgct cacggtgtag taaaaataag gcacctgctt caccgatgtt
     2101 gatgccatca cggtcttggg caaacgggcg acagcgctcg gcgctcaggc tttccaagca
     2161 atcaaagcca ttgaaagtga ggctgcataa agtgtccact cctcccacca acaccgcatc
     2221 gcacacatcg gcctgtaaca aacgctgacc tgccagaatg gctttggctg cagacgagca
     2281 agcagtggaa ac
//
LOCUS       DAOMVK010000369         2291 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_13841_length_2291_cov_3.317084, whole genome shotgun sequence.
ACCESSION   DAOMVK010000369 DAOMVK010000000
VERSION     DAOMVK010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13841_length_2291_cov_3.317084"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1421
                     /gene="ligA"
                     /locus_tag="PK856_10365"
     CDS             <1..1421
                     /gene="ligA"
                     /locus_tag="PK856_10365"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRL02811.1"
                     /translation="EVLGFVSRAPRFAIAHKFPAEEAPTLVEAIDVQIGRTGAVTPVA
                     RLVPVRVGGVTVTNATLHNESEVQRKDVRVGDTVMVRRAGDVIPEVVRVVFDKRPVNE
                     NPGADLFTPATEAKFERYLLPTVCPVCGGGMACQAQRAQALIHFASRTAMDVEGLGNR
                     QIEQLVDLDLVHEFADLYRLDIPTLQRLKQVDEEEGQTSKRETPTKWAENILTGLEAS
                     KTPLLARFIYALGIRHVGERTAKTLAQYFGSLAVIRQVPAPLLACLPDIGEVVAASID
                     QYFAQAYQQQQIDDLLAVGIAPQENPAHAQLLQVLATQNWLTHLPDIRISAKKAEALS
                     AEASAVAALREREVSDSAWLLWREQEQHQALLARIEACLALCETQTSAQTDLQAAIEA
                     LPVNDAIANKTFVLTGTLSNKREEVQAWIEAVGGKVSGSVSKKTDYVVAGEAAGSKLA
                     KAEALGITVLNEQQILEMLGKG"
     gene            1421..>2291
                     /gene="galU"
                     /locus_tag="PK856_10370"
     CDS             1421..>2291
                     /gene="galU"
                     /locus_tag="PK856_10370"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HRL02812.1"
                     /translation="MQKITKAVFPVAGMGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     AVEAGCTDIVFITGRNKRSIEDHFDKAYELETELERKQKNALLEITQNILPEGVNLMY
                     IRQTEALGLGHAVLCAAPAVSNEPFAVILADDLIDAPKGALAQMMDVYNATGSSVLGV
                     ETVDPSQTGSYGIVEIEPKEVYQRVTSIVEKPKPADAPSNLAVVGRYILTPAIFDLLR
                     HTQAGAGGEIQLTDAIAALLKQETVLAHAFSGQRYDCGSKLGYLQATLAYGCKHPEVG
                     AEFAKMVASYKAMS"
BASE COUNT          569 a          518 c          681 g          523 t
ORIGIN      
        1 aagaggtctt gggctttgtg tcgcgtgcgc cgcgttttgc gattgcgcac aaattccctg
       61 ctgaagaagc accgactttg gtggaagcca ttgatgtaca aatcggccgc acgggggcgg
      121 tgacgcctgt ggcgcgtttg gtgcctgtgc gtgtgggcgg tgtgaccgtg accaatgcca
      181 ctttgcacaa cgaaagtgaa gtgcagcgca aagacgtgcg cgtgggcgac accgtgatgg
      241 tgcgccgtgc aggcgatgtg attcctgaag tggtgcgcgt ggtgtttgac aagcggcctg
      301 taaacgaaaa cccaggtgcg gatttgttca cgcctgccac tgaggccaaa tttgagcgct
      361 acttactgcc cacagtttgt cctgtgtgtg gcggcggcat ggcttgccaa gcccagcgtg
      421 cgcaagcctt gattcatttt gcttcccgca ccgccatgga cgtggaaggc ttgggtaacc
      481 gccaaatcga gcaattggtg gatttggatt tggtgcatga gtttgccgat ttgtaccgtt
      541 tggacatccc cactttgcag cgcttgaagc aagtggatga agaagaaggc caaaccagca
      601 aacgcgaaac cccaaccaaa tgggcggaaa acattttaac gggcttggaa gcgagcaaaa
      661 caccgctgtt ggcgcgcttt atctacgcct tgggcatacg ccatgtgggt gagcgcaccg
      721 ccaaaacttt ggcgcaatat ttcggcagtc tagctgtgat acgccaagtg cctgcgccgt
      781 tgttggcgtg tttgcctgac ataggcgagg tggttgcggc aagcatagac cagtatttcg
      841 cacaggcgta tcagcaacag caaatagacg atttgctggc ggttggcatt gctccgcagg
      901 aaaaccctgc tcatgcgcag ttgttacagg tattggctac acaaaactgg ctgacccatt
      961 tgcccgacat ccgcatcagt gccaaaaaag ccgaagcttt gagtgctgaa gcgtcagctg
     1021 tggcggcttt gcgcgagcgt gaagtcagcg acagcgcatg gttgttgtgg cgtgagcaag
     1081 aacaacacca agccttgttg gctcgcatag aggcttgctt ggccttgtgt gaaacacaaa
     1141 cgagcgcgca aaccgattta caggctgcta tcgaagcctt gcctgtgaat gatgcgattg
     1201 caaacaaaac cttcgtatta acaggcacgt tgagcaataa acgcgaagaa gtgcaggctt
     1261 ggatagaggc ggtgggcggt aaagtcagcg gaagtgtgtc caagaaaacc gattatgtgg
     1321 tggcagggga agcggcaggc agcaaattgg ccaaagccga agccttgggc atcacagtct
     1381 taaatgaaca acaaatatta gaaatgttag gaaaagggta atgcaaaaaa tcactaaggc
     1441 agtgtttcca gtggccggca tgggcacacg cttcttgccg gcaaccaagg ctagtccgaa
     1501 agaaatgtta cccatcgtcg ataagccttt gattcaatat gcggtagaag aagcggtaga
     1561 agcaggctgt actgacattg tgttcattac aggccgcaat aagcgcagca tagaagacca
     1621 ttttgacaag gcgtatgagc tggaaaccga gctggagcgc aaacaaaaga atgctttgtt
     1681 ggaaatcacc cagaatattt tgcctgaagg cgtgaatttg atgtacatcc gtcaaaccga
     1741 ggctttgggt ttggggcatg cggtgttgtg cgctgcgcct gccgtgagca atgagccgtt
     1801 tgcggtgatt ttggccgacg atttgattga tgccccgaaa ggtgcattgg cgcaaatgat
     1861 ggatgtgtac aacgccacgg gcagcagcgt gttgggtgtg gaaacggtag acccgagtca
     1921 aactgggtct tacggtatcg tggaaattga acccaaagag gtttaccaac gcgtgaccag
     1981 cattgtggaa aaacccaagc ctgctgatgc accatcgaat ttggcggtgg tgggccgata
     2041 tattttgacc ccagccattt ttgatttgtt gcgccatacc caagcagggg cgggtggtga
     2101 aatccagttg accgatgcaa ttgcagcttt gttaaaacaa gaaaccgtgt tggcgcatgc
     2161 gttcagtggt cagcgttatg actgcggcag caaattgggc tatttacagg ccactttggc
     2221 gtatggttgt aaacatcctg aagttggtgc ggaatttgca aagatggtgg catcatataa
     2281 agcaatgtca t
//
LOCUS       DAOMVK010000370         2290 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_13849_length_2290_cov_3.601790, whole genome shotgun sequence.
ACCESSION   DAOMVK010000370 DAOMVK010000000
VERSION     DAOMVK010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13849_length_2290_cov_3.601790"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /gene="dnaA"
                     /locus_tag="PK856_10375"
     CDS             <1..1037
                     /gene="dnaA"
                     /locus_tag="PK856_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRL02813.1"
                     /translation="NPAEQTRLNPSYTFETLVQGKGNRMAYAGAQTIAENPGSSMYNP
                     YFIYGSTGLGKTHLIQAIGNRIYQQHPRAKIRYIHAETYVRDIMKAFRSSSFDEFKRH
                     YHSLDLLLIDDVQFISGKDRTMEEFFYLFNHLLDEKKQVVMTCDTLPANIKNMDDRLK
                     SRFSWGLTVELEPPELEMRVAILQKKAEAAHVQLQEKAAFFIAQHIRSNVRELEGAFK
                     RVAAHSLFQHKPIDIELATEALQDILVEAQRQITVEHIMKTVGDFYQLKIADLIGKKR
                     TRTIARPRQMAMALAKDLTQQSLPSIGQVFGGRDHTTVMHAHKTIHELKQTDAKLAQD
                     YDYLSAMLQK"
     gene            1051..2157
                     /gene="dnaN"
                     /locus_tag="PK856_10380"
     CDS             1051..2157
                     /gene="dnaN"
                     /locus_tag="PK856_10380"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRL02814.1"
                     /translation="MLILQASRDDLLKPLQTVTGIVERRHTLPILSNVLIEAQAGHTK
                     LLATDLEIQIHTTGPETSSEDFRITTSAKKLQDILRALPESAGVKLDWADNRLTLTAG
                     KSRFNLQTLPAEDFPMMNVAQEVNATFSLRQDQLKLMLSQVQYAMAIQDIRYYLNGLL
                     LQTEGNQFRLVSTDGHRLAFAGITLEDANLPKAEVILPRKTVLELFKLLSHPEDNITV
                     ELLSNQVRVQSGDTVVVSKVVDGKFPDFTRVIPLDNDKLFLINRTTLLGALERAAILA
                     NEKFRGVRLQLKPGLLSVLCSNNEQEEAQEDVEIAYQGEALEVGFNITYLMDVLRNVQ
                     VDDFQLAFGNANQSTLFTIPDNPDFKYIVMPMRI"
BASE COUNT          645 a          582 c          526 g          537 t
ORIGIN      
        1 acaaccctgc cgagcaaacg cgcttgaatc caagctacac gtttgaaacc ttggtacaag
       61 gtaaaggcaa ccgcatggct tatgccggcg cgcaaaccat tgccgaaaac cccggttcaa
      121 gcatgtacaa cccgtatttc atttacggca gcaccggctt gggtaaaacc cacttaatac
      181 aggccatagg caaccgcatt taccagcaac acccacgcgc gaaaattcgc tatatccatg
      241 ccgaaaccta tgtgcgcgac atcatgaagg cgtttcgctc cagttcgttt gacgaattca
      301 agcgccatta ccattcgttg gatttgttgt tgatagacga tgtgcagttt atttcaggca
      361 aagaccgcac catggaagaa ttcttctatt tgtttaacca tttgttggat gagaaaaaac
      421 aagtggtgat gacgtgcgat accttgcctg ccaacatcaa aaacatggac gaccgtttga
      481 agtcgcgctt ctcttggggg ttgaccgtag agttagagcc gcctgaattg gaaatgcgcg
      541 tggcgatttt gcagaaaaaa gccgaggctg cacacgtgca attgcaagaa aaagccgctt
      601 ttttcatcgc ccaacacata cgctccaacg tgcgtgagct tgaaggcgca ttcaaacgcg
      661 tggctgctca cagcttgttc cagcacaagc ccatagacat cgaattggcc accgaagcct
      721 tgcaagatat tttggtagaa gcgcagcgcc aaatcacggt agaacacatc atgaaaaccg
      781 tgggtgactt ttaccaattg aaaattgccg acttaatcgg caaaaagcgc acccgcacca
      841 tcgcccgccc gcgccaaatg gccatggcct tggccaaaga cttgactcaa caaagcttgc
      901 cttccatagg ccaagtgttt ggcggtcgcg accataccac ggtgatgcat gcgcacaaaa
      961 ccatacacga attgaaacaa accgacgcga aattggcaca ggattatgat tacctgtcag
     1021 ccatgttaca aaaataatca gggataaatt atgttgattt tacaagcctc acgcgacgac
     1081 ttactcaaac ctttgcaaac agtgacgggc attgttgaac gccgccacac cttgccgatt
     1141 ttgtctaacg tgctgattga agcccaagca ggccacacca aacttttggc caccgacttg
     1201 gaaattcaaa tccacaccac aggccctgaa acctctagcg aagattttcg catcaccacc
     1261 agcgccaaaa agctgcaaga cattttgcgc gcgctgcctg agtctgcggg tgtgaaattg
     1321 gattgggccg ataaccgcct gaccttaacc gcaggcaaat cacgcttcaa tttgcaaacc
     1381 ttgccagccg aggatttccc gatgatgaat gtggcacagg aagtcaacgc cacctttagc
     1441 ctccgccaag accaattgaa attgatgttg tcacaagtgc aatacgcgat ggcgatacaa
     1501 gacatacgct attacttaaa tggtttgttg ttgcaaaccg aaggtaatca attccgcttg
     1561 gtgtctaccg atggtcatcg cttggccttt gctggcatca ccttggaaga tgccaacttg
     1621 ccgaaagccg aagtgatttt gccgcgtaaa accgtgttgg aattgttcaa attattgagc
     1681 catcccgaag acaacatcac cgtggaattg ctttctaacc aagtgcgcgt gcaaagcggc
     1741 gacaccgtgg tggtttctaa agtggtggat ggcaaattcc cagacttcac ccgtgtgatt
     1801 ccgctcgaca atgacaaatt gttcttgatt aaccgcacca cgctcttggg cgcattggaa
     1861 cgcgccgcca ttttggcgaa tgaaaaattc cgcggcgtgc gcttgcaatt gaaacccggt
     1921 ttgctcagcg tgctttgcag caacaacgaa caagaagaag cgcaagaaga tgtggaaatt
     1981 gcttaccaag gcgaggcttt ggaagtgggt ttcaacatca cgtatttgat ggacgtgtta
     2041 cgcaacgtcc aagttgacga tttccaattg gcgtttggca atgccaatca atcgacattg
     2101 tttaccattc cagataatcc tgatttcaaa tacatcgtaa tgccaatgcg catttaaatc
     2161 ccatgtatca caagcggcct gtaaggctta caggccgctt gaatcggctt atccattaac
     2221 agaagaagtg acttatgact caaccagaag aatacggtgc tgacagcatt aaagtgttaa
     2281 aaggtttgga
//
LOCUS       DAOMVK010000371         2275 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_13971_length_2275_cov_2.907658, whole genome shotgun sequence.
ACCESSION   DAOMVK010000371 DAOMVK010000000
VERSION     DAOMVK010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13971_length_2275_cov_2.907658"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PK856_10385"
     CDS             complement(<1..397)
                     /locus_tag="PK856_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003741486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRL02815.1"
                     /translation="MNAFASLGLSNEIVQALDEQGYTTPTPIQSAAIPKALAGHDLLA
                     AAQTGTGKTAAFMLPSLERLKRFANTSASPAMHPVRMLVLTPTRELADQIDANVTSYI
                     KYLPLKHTVVFGGTNMDAQSQALRSGREIV"
     gene            complement(466..1221)
                     /locus_tag="PK856_10390"
     CDS             complement(466..1221)
                     /locus_tag="PK856_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009119364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HRL02816.1"
                     /translation="MIKTQKLFEQIALVLEERIVRGVYTAKSKLPPERQLAEEFGVSR
                     PSIRDALRVLTTRELLEARQGDGYYVSDQLQRAFVGGWQDLLQHHEYLESDVLDFRRS
                     LEGMLAAVAAERRTDTDLERIAYWLQQIDVAVAQKSVKLQSQADVNFHQAIAEASHNV
                     LFIQLSASLLRILHQHTQQNLANMFSVDDDAKLILRVQHWAILEAIRNKDSEQARQLA
                     SGHIDYVEDSLAHYRQMYQREQQAQQLAHKDMR"
     gene            1422..2204
                     /locus_tag="PK856_10395"
     CDS             1422..2204
                     /locus_tag="PK856_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRL02817.1"
                     /translation="METSNNRFEHVTDVYFFGTCVLDIFMPEAGVDAMTLLEQQGIRV
                     HYPQEQSCCGQPAYSSGRPKEAFAVAKEQLKLFPQPWPIVVPSGSCGGMMKHHWPTLF
                     DGTEFERQANEISSRVFEWSRFLVDVLHYTPKDTGVPIKVAVHTSCGARREMNVHTSS
                     WALVDALEHVVRVVHDHESECCGFGGTFSMKHPDISGAMVSDKVQALKDAQATAIISA
                     DAGCMLNIGGKIAKDDPSIPKPKHLATFLLERTGGKPYEEQA"
BASE COUNT          575 a          559 c          544 g          597 t
ORIGIN      
        1 ccacgatttc acgaccgctg cgcaaggctt gcgattgggc atccatattg gtgccgccaa
       61 acaccaccgt gtgttttaaa ggcaggtatt tgatgtaaga cgtgacattg gcatcgattt
      121 ggtcggccaa ttcacgtgtc ggcgtcaaca ccaacatgcg cactggatgc atcgcaggtg
      181 aagcactggt gttggcgaag cgtttcaagc gctccaagct tggcaacatg aatgcggcgg
      241 ttttgcctgt gcccgtttgt gcggctgcca acaaatcgtg gcctgccaat gctttgggta
      301 tggcggctga ttgtatcggt gttggtgtgg tgtaaccttg ttcgtccaaa gcttggacaa
      361 tttcatttga taaacccaat gatgcaaaag cattcatgct tttaaaaccc cttgtgttgc
      421 ggttacaggc ggcctgtaac cgaataattt gtgcgacttg cgtgcttaac gcatgtcttt
      481 gtgtgccaat tgttgtgcct gttgttcccg ttggtacatt tggcgatagt gggccaaaga
      541 gtcttccacg taatcaatgt gaccgctggc caactgacgg gcctgctcgc tgtctttgtt
      601 acgtatcgcc tctaagatgg cccaatgttg cacgcgcaaa atcagtttgg catcgtcgtc
      661 caccgaaaac atattggcca agttttgttg ggtgtgttgg tgcaagatgc gcagcaagct
      721 ggcagacaat tgaatgaaca acacattgtg tgacgcttcg gcaatggctt gatggaaatt
      781 cacatctgct tgcgactgca atttcacgct tttctgcgcc acggccacat caatttgttg
      841 taaccaataa gcaatgcgtt ccaaatcggt gtcggttcga cgctctgccg ccaccgcagc
      901 caacatccct tctaaggaac gcctgaagtc taacacatca ctctctaaat attcatggtg
      961 ttgcaacaaa tcttgccatc caccgacaaa agcgcgttgc aattggtcgg aaacataata
     1021 gccatcgcct tggcgtgctt cgagtaactc gcgcgtggtc aacacacgca aggcatcgcg
     1081 aattgacgga cgcgacacgc caaactcttc ggccaattgc cgctcgggtg gcaatttgga
     1141 tttggcagtg tagacgccac gcacaatgcg ctcctccaaa accaaggcaa tttgctcaaa
     1201 taatttttgg gtttttatca cgctgccacc tcttttttac atttcgcaat gaaaataaaa
     1261 taaacgattg attttaaata aaataattaa aataaaatct gaaactttgc ctggtttgtt
     1321 aaggtttgtg tgcaatcaag gattgaccta aacatcagat atttgtaaag tatttgtaaa
     1381 taaaaaaatt ggtctgacca atttaccaag gataacatag aatggaaacc tcaaacaacc
     1441 gttttgaaca cgttactgat gtgtattttt ttggtacctg tgtgctggac atcttcatgc
     1501 cagaagctgg tgtggatgcc atgacgctac tggaacaaca aggcatacgt gtgcactatc
     1561 cacaagagca aagctgctgt ggacagcccg cttattcttc aggccgccct aaggaagcat
     1621 ttgcagtagc caaagagcaa ttgaaattgt tccctcaacc ttggcccatt gtggtgcctt
     1681 ctggctcttg cggtggcatg atgaaacacc attggccaac cctttttgat ggcaccgaat
     1741 ttgagcgtca agccaatgaa atttccagcc gtgtgtttga atggtctcgt ttcttggtgg
     1801 atgtgttgca ttacactccc aaggacacgg gcgtgcccat caaagtggcg gtacacacca
     1861 gttgtggtgc acgccgtgaa atgaatgtgc acacctcaag ctgggccttg gtcgatgctt
     1921 tggaacatgt ggtgcgcgtg gtacacgacc acgaaagcga atgctgtggt tttggtggta
     1981 ccttctcgat gaaacacccc gacatctctg gtgctatggt cagtgacaaa gttcaagccc
     2041 taaaagacgc ccaagccacg gccatcatca gtgccgatgc tggctgtatg ctcaacatcg
     2101 gcggcaaaat tgccaaagat gatccttcca tacccaaacc caaacacttg gcaaccttct
     2161 tattggaacg cacaggtggc aaaccttacg aggagcaagc ctgatgagcg cccgtgacaa
     2221 tatttttgcc aaactccgtg ccgccaatgc caaacctttg gccgaaccac aaacc
//
LOCUS       DAOMVK010000372         2254 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_14138_length_2254_cov_3.196453, whole genome shotgun sequence.
ACCESSION   DAOMVK010000372 DAOMVK010000000
VERSION     DAOMVK010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14138_length_2254_cov_3.196453"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="PK856_10400"
     CDS             <1..500
                     /locus_tag="PK856_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02818.1"
                     /translation="PEDYVWFLSQVGNGGAGPFYGLYALEDSLEVHTDFGSPMSLNSP
                     FPFTQATLPDEIQLSLLEDQLGAAQEAGDEDLETELYEQYQALLFPSEWANGYLHLCD
                     YGCGISFFLVIAGEDAGTVWENRAVDAAGLMPSTEFGNTVKLGFLEWYQLWLDQALAN
                     LSTVN"
     gene            638..1819
                     /locus_tag="PK856_10405"
     CDS             638..1819
                     /locus_tag="PK856_10405"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRL02819.1"
                     /translation="MNIHFNCVFLAIILGLAACSDSSESNHTTPSPTTSQPSQVASQP
                     KNELKTADIAVAYIPPIIVPDFLSMTPQQWQLQKLLPASFNPSSGIQVKPAPCKDPKT
                     NANSAATQVIKTDQGTLLIGEGSGGLFSSDQLTISLNDDGTGVLVAKLDDGTSFTVYG
                     SNNGKGSYVGPEGRFDINGDGSGTWRKGSTTVTINKNGSATLHDERGLLTIKPNGSGT
                     WQRNGGNAEYIENFGDGTGVLGSTNPIKVKMAPWQVPPPVAAMPPIQKLPKPTASCGY
                     IINLAENILFDYDKHELNPAAYPLLDELAQALQQTKVDQIEILGHTDSKSSDDYNLRL
                     SLQRAQAVLQALQQRHSADHATAHGYGEAQPVAPNEVDGTDSPSNRQLNRRVEIFVKT
                     Q"
BASE COUNT          635 a          568 c          497 g          554 t
ORIGIN      
        1 tacctgaaga ttatgtgtgg tttttaagcc aagtgggcaa tggcggcgca ggcccatttt
       61 acggcttgta tgccttagaa gacagcttgg aagtacacac cgattttggc agccccatga
      121 gcctgaacag cccattccca ttcacccaag ccaccttacc agatgaaata caactatcac
      181 tgttagaaga ccaattagga gccgctcaag aagcaggtga tgaagacttg gaaaccgaac
      241 tgtatgaaca ataccaagcc ttgttgttcc cttcagaatg ggcgaatggc tatttgcact
      301 tgtgtgacta tggctgtggc atcagtttct ttttggtgat tgcaggcgaa gacgctggca
      361 cagtgtggga aaaccgcgcc gttgatgctg caggccttat gccctctact gaatttggca
      421 atacagtgaa attgggcttt ttagagtggt accaattgtg gctagaccaa gccttggcca
      481 atctgagcac ggtcaattaa atcacctatg atgcacacaa gccttatcac gaccactgtc
      541 gtcatttggc ctgtgagcat agtcgctgtg tggccgtcct taagtattga ttcttgctat
      601 actgccttag caaaaactct aacaagaggc atccgtgatg aacatccatt tcaattgtgt
      661 attcttagct atcatattgg gcttggcagc ctgctcagat agctctgaat ctaatcacac
      721 cacaccaagc ccaacaacca gccaaccctc acaagtcgca agtcagccca agaatgagct
      781 caaaaccgcc gacatagccg tggcttacat tccacccatc atcgttcctg attttttaag
      841 tatgacgcca caacaatggc aattgcagaa gctgttaccc gcctcattca atccaagcag
      901 cggcatacaa gtcaaacccg caccttgcaa agacccaaaa accaatgcca atagcgcagc
      961 aacccaagtt attaaaacgg atcaaggcac tttattgatt ggtgaaggca gcggtggctt
     1021 gttcagcagc gatcaactga cgatttcact gaacgatgat ggaacaggtg tgttggtggc
     1081 caaattagac gacgggacca gtttcacagt ttatggtagc aacaatggca aaggcagcta
     1141 tgttgggcca gaaggccgat ttgacatcaa tggcgatggc agcggtactt ggcgtaaagg
     1201 cagcaccaca gtcaccatta acaaaaatgg ctccgccacc ctacacgatg aacgaggctt
     1261 attaaccatt aaacccaatg gttcaggcac gtggcaacga aacggtggca atgccgaata
     1321 catcgaaaac tttggcgatg gaacaggtgt attgggcagc acgaatccaa ttaaagtgaa
     1381 aatggctccg tggcaagttc ccccgcctgt tgcagccatg cccccgattc aaaaactccc
     1441 caaaccaact gcctcatgtg gctacatcat caatctggct gaaaacatat tatttgatta
     1501 cgacaaacat gagctcaatc ccgcagccta tcccctactg gatgaattag cgcaggcatt
     1561 gcaacaaaca aaagtggatc aaattgaaat tttgggccac accgacagca aaagcagtga
     1621 tgattacaat ctgcgcctgt cacttcaacg cgctcaagcc gtattacaag ccttgcaaca
     1681 acgccacagt gccgaccatg ccacagccca cggttatgga gaagcacaac ctgtcgcacc
     1741 gaatgaagtc gatggcacag acagccccag caaccgccag ctcaatcgcc gcgtagaaat
     1801 tttcgtcaaa actcagtaat tgactgtgct cttctgcttc ttgataagtt ccatcatgat
     1861 tattgactct atcaaagcgg cctgtaccag tttacaggcc gctttcgctt atagtacgag
     1921 gcattggagt atcaattagg tttgactcaa attctcaccc acctggagcc tgtttattgc
     1981 tcataacctt caacacagct ttcttacaaa cgacggctat tctaaccatc aaatgattta
     2041 cttgataaac aagcggccag taaacgtgtt ttaagtgctt acaggccact tgtgttgatg
     2101 cttgaagtta atggtttggc acaaagtctt cgacacacag tgcgaacgca tctattcatg
     2161 ccttgcttta tgattttgtg ttttggtttt accaatgagc aaagccctga tgctatgcat
     2221 cagggctttg gaatggggag tttggcggtg acct
//
LOCUS       DAOMVK010000373         2247 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_14202_length_2247_cov_2.559763, whole genome shotgun sequence.
ACCESSION   DAOMVK010000373 DAOMVK010000000
VERSION     DAOMVK010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14202_length_2247_cov_2.559763"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="PK856_10410"
     CDS             complement(<1..718)
                     /locus_tag="PK856_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HRL02820.1"
                     /translation="MLQQHADKLKQLIAPHYQGEVEQFASPSDAYRMRAEFRVWHQEG
                     KPHYAVYNTDNPEGKRQIQIIESWPIVHESIAAIMPKLLAATVGNTVLTQKWFQVEFL
                     ATLSGDMLITMVYHRALDAEWEATARALAAQLGVHIIGRSRKQKIVLTQDYVTEALEV
                     TGRTYRYRQIEGGFTQPNAWVCEKMLAWAVEAAGKNERDLLELYCGNGNFTLPLSRCF
                     RQVLATEISKTSVHAAEWSIA"
     gene            1006..1440
                     /gene="trxC"
                     /locus_tag="PK856_10415"
     CDS             1006..1440
                     /gene="trxC"
                     /locus_tag="PK856_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HRL02821.1"
                     /translation="MNENLHYVCPHCHSTNRVPVSKLSEHPTCGHCKEALITGSPVAL
                     NTDSFSRHVMRSQFPVVVDFWAPWCGPCQSMAPAFAEAAERLKLTAQLCKLDTEAYPQ
                     TAAPYGIRSIPTMVVFREGQEIARVSGAMPVGEIERWVKSVL"
     gene            complement(1520..1717)
                     /locus_tag="PK856_10420"
     CDS             complement(1520..1717)
                     /locus_tag="PK856_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02822.1"
                     /translation="MQGKKYWPVKHQFSKEDKVYYQIMEFDARAEPKPYVGEEANVLV
                     FEAFNDAIKYIRERNLSQLLT"
     gene            complement(1951..>2247)
                     /locus_tag="PK856_10425"
     CDS             complement(1951..>2247)
                     /locus_tag="PK856_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02823.1"
                     /translation="DLVQIHAYCSEIGNMAMNVMALRQQNIALPKAERVIRTQFQYDL
                     ENSEVSQYLSEALETAYAIPIERKVGQKFQQAEWHRLAFMDKCLVTIMSRESGN"
BASE COUNT          604 a          552 c          464 g          627 t
ORIGIN      
        1 ccgcaatact ccattccgca gcatgcactg atgttttgga aatttcagtg gccaagactt
       61 ggcgaaagca acgcgacaaa ggcaaggtga aattgccatt gccacaatac aattcgagca
      121 aatcgcgttc atttttaccc gctgcttcca cggcccaagc aagcattttt tcgcacaccc
      181 acgcattcgg ttgggtaaag ccaccttcga tttggcggta gcggtaagtg cggcctgtaa
      241 cttctaatgc ttcggtcaca tagtcttggg tcaacacgat tttttgtttg cggctgcggc
      301 cgatgatgtg cacacccaat tgcgctgcca aagcgcgcgc agtggcctcc cattcggcat
      361 ccagtgcgcg gtggtaaacc atggtaatca acatgtcacc gcttaaagtg gccaaaaatt
      421 ccacttgaaa ccacttctgc gtcaacactg tgttgcccac cgtggcggcc aataatttgg
      481 gcatgatggc ggcaatggat tcgtgcacaa tcggccacga ttcaatgatt tggatttggc
      541 gtttgccttc aggattgtcg gtattgtaaa ccgcgtaatg gggtttgccc tcttgatgcc
      601 agacccgaaa ctcagcacgc atgcgatacg catcgcttgg cgatgcaaat tgttccactt
      661 cgccttggta atggggcgcg attaattgct tgagcttgtc ggcatgttgt tgcagcacgt
      721 tggacattcc tgaataaaag gctagattat aatgcccgca ccgcgactta accacacgaa
      781 aaacacggac catcgcatta ttttgtgttg taggtgcatg caagcggcct gtaaagtctc
      841 atttgcgttt gaatacccaa ttaacggagt attcctacac attacccgta tcttctatga
      901 ggcttacagg ccgcttaaat attgccactg tgattatgga ttgcttatca ttgagacctt
      961 gctccatgcc ttgttcacat cgtgccatcc atttaaagga aacccatgaa cgaaaacctc
     1021 cattacgtgt gcccccattg ccattctacc aaccgtgtac ctgtgtccaa actctcggaa
     1081 cacccaacat gtggccattg taaggaagcc ttaatcacag gctcacctgt ggcattgaac
     1141 acagacagct tttcacgcca tgtgatgcgc agccaatttc cagtggtggt ggacttttgg
     1201 gcgccatggt gtggtccttg ccaatctatg gccccagctt ttgctgaagc tgccgaacgt
     1261 ttaaagctga ccgcacagct gtgtaaactc gataccgaag cctatccaca aaccgctgcg
     1321 ccatatggca tacgcagcat tcccaccatg gttgtgttca gagaaggaca agagattgcc
     1381 agagtttctg gagccatgcc tgttggggaa attgagcgtt gggtaaaatc ggtattgtga
     1441 tggaatgctc ttgctataga tttaagagag gacattcata caaaaagcca ctgtcacagt
     1501 ggctttaagg tctttgcaat taagtgagta attgactgag attgcgttca cgtatgtatt
     1561 taatggcatc attgaatgcc tcaaaaacca aaacattcgc ttcttcaccc acataaggct
     1621 tgggctcagc ccgagcatca aactccatga tttggtaata gaccttgtcc tctttagaaa
     1681 actggtgctt cactggccaa tattttttcc cttgcacggt caatactccc ccaattaaaa
     1741 gaagtaatga cttcatgtta atactctaaa tgagttgatt caagtattca gcataaaatc
     1801 acagcaaata agcagcttcg caccatgcca aaacatttaa ttgcaacaaa tagatagaaa
     1861 atcccaataa atgaaacaaa atttcaatta aagaaaaata attttaaatt tatgcccaaa
     1921 attcacacat cactttaacc gttttatttt tcaatttcca ctttccctgc tcatgatagt
     1981 tactaagcac ttatccataa atgccaaccg atgccattct gcctgctgaa atttttgacc
     2041 cacttttcgt tctatgggaa ttgcataagc cgtttccaaa gcctcgctca aatactgtga
     2101 tacttcgcta ttttctaaat cgtattggaa ctgtgtccga ataaccctct cagctttagg
     2161 taatgcaatg ttttgctgcc ttaaagccat cacattcatg gccatgttgc ctatttcaga
     2221 acagtacgca tgtatttgaa ccaaatc
//
LOCUS       DAOMVK010000374         2242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_14240_length_2242_cov_2.758116, whole genome shotgun sequence.
ACCESSION   DAOMVK010000374 DAOMVK010000000
VERSION     DAOMVK010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14240_length_2242_cov_2.758116"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1492
                     /gene="thiC"
                     /locus_tag="PK856_10430"
     CDS             <1..1492
                     /gene="thiC"
                     /locus_tag="PK856_10430"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HRL02824.1"
                     /translation="QSSAYGRERLSNNELDGIRFEHIRKPRRAQAGKNVTQMHYAKQG
                     IITPEMEFIAIRENMHRYQVRNEVLQQQHAGQPLGAMIPADITPEFVRQEVAAGRAII
                     PNNINHPETEPMIIGRNFLVKVNANIGNSALGSSIDEEVAKMTWATRWGADTIMDLST
                     GKNIHETREWIIRNAPVPVGTVPIYQALEKVGGVAEDLTWELFRDTLIEQAEQGVDYF
                     TIHAGVLLKYVPLTAKRLTGIVSRGGSIMAQWCLAHGKENFLYEHFDDICDIMKSYDV
                     AFSLGDGLRPGCLQDANDAAQFGELHTLGELTQRAWQHDVQVMVEGPGHVPLHMIRAN
                     MDEQLAACFEAPFYTLGPLTTDIAPGYDHITSAIGAANIAWYGTAMLCYVTPKEHLGL
                     PNKQDVKDGMMAYKIAAHAADLAKGHPGAQVRDNALSKARFEFRWEDQFNLSLDPETA
                     RKMHDETLPKQAQKSAHYCSMCGPKFCSMKISQNVREYAAQLEDMI"
     gene            complement(1699..>2242)
                     /locus_tag="PK856_10435"
     CDS             complement(1699..>2242)
                     /locus_tag="PK856_10435"
                     /inference="COORDINATES: protein motif:HMM:NF015558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HRL02825.1"
                     /translation="IFLHPVLLKQMIAWGWIGGQPILQGLFIGSSVHEVAQVVAIGKA
                     IDPNTAQMAVIVKMVRVMLLAPFLMMVMWVFSRSSLSHADKAVQTEKTAITIPWFAFG
                     FIAVAGLNSLQLLPSVLVHAIQAFDVLLLCIAMVALGFTTRISIIRKAGVKPILLASV
                     LFVLLLCLSTLSVYLLNPYY"
BASE COUNT          619 a          632 c          537 g          454 t
ORIGIN      
        1 acaatcctcg gcatatggcc gtgaacgctt gtccaacaat gaattggatg gcatccgctt
       61 tgaacacata cgcaagccac gccgcgccca agcaggcaaa aacgtcaccc aaatgcatta
      121 cgccaaacaa ggcatcatca cgcctgaaat ggaattcatc gccatacgcg aaaacatgca
      181 ccgctaccaa gtgcgcaatg aagtcttgca gcaacagcac gcaggccaac ccttgggcgc
      241 gatgatacct gccgacatca cgcctgaatt tgtgcgacag gaagtggccg ctggccgcgc
      301 catcatcccc aacaacatca accatcctga aaccgaaccg atgattatcg ggcgcaattt
      361 cttggtgaag gtcaatgcca acattggcaa ttcggctttg ggctcgtcta tcgatgaaga
      421 agtggcaaaa atgacttggg ccacacgctg gggcgccgac accatcatgg atttgtctac
      481 gggcaaaaac atccacgaaa cacgcgaatg gatcatccgc aacgcgcccg tgcccgtggg
      541 caccgtgccg atttaccaag ccttggaaaa agttggcggc gtggccgaag atttgacgtg
      601 ggaattgttc cgcgacacct taatcgagca agccgagcaa ggcgtggact atttcaccat
      661 ccatgcaggc gtattactga aatacgtgcc cttaaccgcc aaacgcctca caggcattgt
      721 gtcgcgcggc ggctccatca tggcgcagtg gtgcttggcc catggcaaag aaaacttttt
      781 atacgagcat tttgacgaca tttgcgacat catgaaatcg tacgatgtgg ccttcagctt
      841 gggcgatggt ttgcgcccag gctgcttgca agacgccaac gatgccgccc aattcggcga
      901 attgcacacc ttgggcgaac tcacccagcg cgcgtggcaa cacgatgtgc aagtgatggt
      961 agaaggccca ggtcacgtgc ctttgcacat gatacgcgcc aatatggatg agcaattggc
     1021 cgcctgtttt gaagcgccgt tttacacctt aggcccactc accaccgaca tcgccccagg
     1081 ttacgaccac atcaccagcg ccatcggtgc ggcgaatatt gcgtggtatg gcacggcgat
     1141 gctgtgttac gtcacgccga aagagcattt gggcttgccc aacaaacaag acgtgaaaga
     1201 cggcatgatg gcttacaaaa tcgccgccca cgccgccgat ttggccaaag gccacccagg
     1261 cgcacaagtg cgtgacaatg ctttgtcgaa agcgcggttt gaattccgtt gggaagacca
     1321 atttaatttg tcactcgacc cagaaacggc acggaaaatg cacgatgaaa ccttgcccaa
     1381 gcaagcacag aaatcggcgc attactgttc aatgtgtggc cccaagtttt gctcaatgaa
     1441 gatctcccaa aacgtgcgtg aatacgcagc acaattggaa gacatgattt aagcggcctg
     1501 taaacaagct gcacacacac ccacaaaacc acggcacaca ctgccgtggt tttccgattt
     1561 tcgccccacc caaagtcaaa tgcatcgctt atctacaacg tggctttttt tggtgaaaac
     1621 tgtcagcgca aacaaagaaa agccacaaac tcagttcgtg gctttaggct ttacaggccg
     1681 catatcggca gttggccctc aataataagg attcagcaaa tacacactga gggtggacaa
     1741 acacagcagc aacacaaaca acacactggc caacaagatg ggtttcacac ctgcttttct
     1801 gatgatgctg atgcgggtgg taaaacccaa agctaccatg gcgatgcaca agagcaacac
     1861 gtcaaaagcc tgtatggcat gaaccaacac actgggcaac aactgtaaag aattcaaacc
     1921 agcaaccgca atgaagccga aagcaaacca gggtatggta atcgcggttt tttctgtttg
     1981 cacggcttta tctgcatggg acaggcttga gcgcgaaaac acccacatca ccatcatcaa
     2041 aaacggtgcc aacagcatca cccgcaccat tttcactatc accgccattt gtgccgtatt
     2101 ggggtctata gccttgccga ttgccactac ttgcgccacc tcatgcaccg atgagccgat
     2161 gaacagtcct tgtaatatcg gttgcccgcc tatccaaccc caagcaatca tttgtttgag
     2221 caacactggg tgcaggaaaa tg
//
LOCUS       DAOMVK010000375         2240 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_14260_length_2240_cov_2.578947, whole genome shotgun sequence.
ACCESSION   DAOMVK010000375 DAOMVK010000000
VERSION     DAOMVK010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14260_length_2240_cov_2.578947"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..345)
                     /locus_tag="PK856_10440"
     CDS             complement(82..345)
                     /locus_tag="PK856_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HRL02826.1"
                     /translation="MSELQQRFEQAQKDVVSLAERPQNAVLLKLYAYYKQAVDGDVSG
                     DKPGAFDLVAKKKYAAWEEIKGTSNDAAMQLYIDEVQGLLDEE"
     assembly_gap    360..396
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<397..1192)
                     /locus_tag="PK856_10445"
     CDS             complement(<397..1192)
                     /locus_tag="PK856_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003824544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HRL02827.1"
                     /translation="MRIKLITTILFACALSACQPQAATENSKKSAASETGASKAVVSG
                     DMASVKKNIEAALTQAYPDQKVQIKSVEAAPIAGLYEVVVFPNQVVYMDGKGEFMFVG
                     NLLNVKSQTNLTEAKMSDLNRIDYKALPFDSAIKEVRGNGQYKVAVFSDPDCPFCKKL
                     EHEIAQMTDVTIYTFLMPIDSLHPQATEKSIQLWCQPNRTEAWTKWMRDGVAPAQVAV
                     CKNPVAELTKLAEGFGFNGTPTIVYPNGKVQSGYMPKADFEAALKQN"
     gene            1362..1562
                     /locus_tag="PK856_10450"
     CDS             1362..1562
                     /locus_tag="PK856_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003781086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02828.1"
                     /translation="MAQTRLVATGITSKADADKIAQAGEAVEGVRFVNVNHEDGGIVI
                     THSDAFNADTFKSAIQALGFGF"
     gene            1562..>2240
                     /locus_tag="PK856_10455"
     CDS             1562..>2240
                     /locus_tag="PK856_10455"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRL02829.1"
                     /translation="MTAPSAYCPQYAAERHIQHLNGLSHEVLHWPRNSRPKLLLLHGW
                     MDCADTFQALVDALPPQYDIYAFSWRGFGGSQWQHSAYYERMVMLQDLHQLLEHVSPN
                     DAIHVCGHSMGSMLATHYACLFPERIKSLTMAEGFGMPVLNQADELRARARRFVHTPE
                     PNPTYTLKSITDVAEKLCKRNPQMSLALAHHMAKALTQYTEQGLVYRADPKHHLPQAH
                     PYDWHRIA"
BASE COUNT          584 a          558 c          445 g          616 t
ORIGIN      
        1 cgcccactgt tgtttgattc attttatata aataaaaagc cgctttcatt gaaagcggcc
       61 tgtaaacgca tagagtctgt tttactcttc gtctaataaa ccctgtactt catcaatgta
      121 caattgcatt gcagcatcat tgctggtgcc tttaatctct tcccaagccg catacttctt
      181 cttggcaacc aaatcaaaag cacctggttt gtcaccactt acatcaccat ctaccgcctg
      241 tttgtagtaa gcatacaact tcaacaacac cgcattttga ggacgttcag ccaaactcac
      301 cacatctttt tgtgcttgtt caaaacgttg ttgcaattca ctcatgagca ttcttccttn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngatt ttgcttcaat gccgcttcaa
      421 aatcggcttt aggcatgtag cctgattgta ctttgccatt aggataaaca atggttgggg
      481 tgccattaaa accaaaccct tcagcaagct tggtcaattc agccacagga tttttgcaca
      541 cagccacttg tgcaggtgca acgccatccc gcatccactt ggtccaggcc tctgtgcgat
      601 ttggctgaca ccacaactga atggatttct ccgtggcttg tggatgcaat gaatctatgg
      661 gcattaagaa agtataaatg gtcacatcgg tcatttgagc aatctcatgc tccaatttct
      721 tacagaatgg acaatcaggg tcgctgaaaa ccgccacttt gtattgccca ttgccacgca
      781 cttctttaat ggcggaatca aatggcaagg ctttgtagtc tatgcggttc aaatctgaca
      841 ttttcgcttc ggtcaaattg gtctggctct tcacattcaa caaattcccc acaaacatga
      901 attcaccctt accatccatg tacacaactt gatttgggaa caccaccacc tcatacaggc
      961 cggcaattgg agcagcttcc acacttttga tttgtacttt ttgatctggg taggcttgcg
     1021 tcaaagccgc ttcaatgttc tttttcacgc tggccatatc accagaaacc accgctttag
     1081 atgctcctgt ttcagaagcg gcactctttt tgctattttc agttgcagct tgtggctgac
     1141 atgcacttaa ggcacaggcg aagaggatgg tggtaattaa tttaatccgc ataaaattct
     1201 cttaaaattg attgctaaaa attacaaatc ttggcaatat tgtcaaaatg tcaaatggca
     1261 tattgtttta ccttgtaatg attaagtttt tccaccagca tcgtaacata tccaaagatt
     1321 taagtggttc attgttcttc aatcactcgt acggaggctc tatggctcaa actcgtttgg
     1381 tagcaactgg tattacatct aaagcagatg ccgacaaaat tgcgcaagca ggagaagcag
     1441 ttgaaggtgt gcgttttgtc aacgtcaacc atgaagatgg tgggatagtc attacccatt
     1501 ctgacgcatt caatgcagat acattcaaaa gtgccattca agcattggga ttcggttttt
     1561 aatgacggct ccatcggcat actgtcctca gtatgccgct gaacgacata tccaacactt
     1621 gaatggttta agccatgagg tgttgcattg gccgcgaaac tcgcggccaa aattattgct
     1681 gctgcatggt tggatggact gtgccgatac cttccaagcc ttggtagatg ctctgccacc
     1741 ccaatacgac atttatgcat tcagttggcg cgggttcggc ggctcacaat ggcaacacag
     1801 cgcttattac gaacgcatgg tcatgttgca agacttgcac cagcttttag aacacgtcag
     1861 ccccaatgat gctattcatg tgtgcggcca cagcatgggc agcatgttag caacacatta
     1921 cgcctgtttg tttcctgagc gcatcaagag cctgaccatg gccgaaggtt ttggtatgcc
     1981 cgtcctaaat caagctgacg aattgcgtgc cagagcacgc cgctttgtgc ataccccaga
     2041 gcctaaccct acctacacac tcaaaagcat taccgatgtg gcagagaaat tgtgcaaacg
     2101 caatccccaa atgagcttgg ccttagcaca ccacatggcc aaagcactga cccaatacac
     2161 ggaacaagga ttggtttacc gagccgaccc caaacaccac ttgcctcaag cccacccata
     2221 cgattggcat cgcatcgccc
//
LOCUS       DAOMVK010000376         2225 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_14384_length_2225_cov_2.224885, whole genome shotgun sequence.
ACCESSION   DAOMVK010000376 DAOMVK010000000
VERSION     DAOMVK010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14384_length_2225_cov_2.224885"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..756
                     /locus_tag="PK856_10460"
     CDS             100..756
                     /locus_tag="PK856_10460"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HRL02830.1"
                     /translation="MKQTKHWSRVEYLHQTVSNPNIIVKGEHSYYSDAWDDGFEQSVV
                     RYLYGDEESLKWEPLWPIDKLYIGDYVCIGAEAVILMGGNHTHRADWFSLYPFMDTIE
                     AAYVGKGDTHIGDGAWIGMRAMIMPGVNIGEGAIIAANSVVTKDVAPYCVVAGSPAKQ
                     LKTRFEPEVIVQLLQLNIYDWPAEKFQALQPYLSQGNLEVLLAQEQQYAAEQDGQLGK
                     "
     gene            complement(835..1590)
                     /locus_tag="PK856_10465"
     CDS             complement(835..1590)
                     /locus_tag="PK856_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRL02831.1"
                     /translation="MIDAQHIRKSFGALEVLKDVNLQVRQSEVVAIIGSSGSGKSTLL
                     RCLNHLETINGGSIAIEGQTMVNSDAAGHAQYKADKEVKALCSKMGMVFQQFNLFPHM
                     TVIQNIIEAPMTVKKMRKEQILPLAHELLAKVGLAEKHDFYPSQLSGGQKQRVAIARA
                     LAMQPDIMLFDEPTSALDPELTGEVLKTMQKLAEDNMTMVVVTHEMGFAREVADQVYF
                     MDGGVMVEGGEAKAFFDNPQHPRTKAFLSNMLK"
     gene            complement(1595..>2225)
                     /locus_tag="PK856_10470"
     CDS             complement(1595..>2225)
                     /locus_tag="PK856_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRL02832.1"
                     /translation="QMLPPMWEGAQMTLKLFAITLLLSIPLGLILALLRLSGIKALDV
                     FVNGYIWLMRGTPLMLQMLFVYFALPAVGIYFEDFTAAVVAFALNYAAYFAEIFRGGI
                     LAIGRGQYEAAKTLGMSYPQTMRRIVLPQMWKKILPPLSNETITLVKDTSLVYVLALN
                     DLLRATRNIVQRDFNVTPFLTAAAFYLIMTLILTWMFQYMEKRYAVYED"
BASE COUNT          572 a          515 c          600 g          538 t
ORIGIN      
        1 ttcctgaatg aggtgtgcaa atgatgctct gtgataaacc cacagctgac atgtcggcag
       61 gtaggatagc gtttagaaac gatattgaaa gtataaaaca tgaagcaaac caaacattgg
      121 tcgcgggtgg aatacctgca tcagaccgtg agcaatccga acatcatcgt caaaggtgag
      181 cacagctatt acagtgatgc ttgggacgat gggttcgagc agtcggttgt gcgctatttg
      241 tatggcgatg aggaaagcct caaatgggag cctttgtggc caatagacaa gctgtacatt
      301 ggtgactatg tgtgcatagg ggctgaggcc gtgattttga tgggtggcaa tcacacccac
      361 cgtgccgatt ggttcagcct gtacccattc atggacacca tagaagcggc ttatgtgggc
      421 aagggcgaca cgcacattgg cgatggcgca tggataggca tgcgggcaat gatcatgcct
      481 ggggtgaaca ttggtgaggg agccatcatc gctgccaaca gcgtggtgac caaagacgtg
      541 gcgccgtatt gtgtggtggc gggttctcct gccaaacagc ttaaaacgcg atttgagcct
      601 gaggtgattg tgcaattgtt gcaattgaac atttacgatt ggccagcaga aaaatttcaa
      661 gccttgcagc cgtatttgag tcagggcaat ttggaggtgt tgttggcgca agagcaacaa
      721 tatgcagctg agcaagatgg tcagctaggt aaatgaacag catctgctga cacacaagcg
      781 gtctgtaaat agtttacagg ccgctttgtt agtgagagta agaaaatatt tcgcttactt
      841 caacatattg ctcaagaaag ccttggtgcg cgggtgttga gggttgtcga agaaggcttt
      901 ggcttcgccg ccttctacca tcacgccgcc gtccatgaaa tacacttggt cggccacttc
      961 gcgggcaaag cccatttcgt gtgtgaccac caccatggtc atgttgtctt cggccagttt
     1021 ttgcatggtt ttcaacacct cgcctgtgag ctcggggtcg agggcggaag tgggctcgtc
     1081 aaacaacatg atgtcgggct gcatcgccag ggcgcgggcg atggcgacgc gctgtttttg
     1141 accgccagac aattgcgatg ggtaaaaatc gtgtttttca gccaatccga ctttggcgag
     1201 caattcgtgc gccagcggca aaatctgctc tttgcgcatt tttttcaccg tcatcggcgc
     1261 ttcaatgatg ttttgaatta ccgtcatgtg tgggaacaaa ttgaattgct gaaacaccat
     1321 gcccattttg ctgcacaagg ctttcacttc tttgtcggct ttgtattggg cgtgacctgc
     1381 ggcatcgctg ttgaccatgg tttggccctc aatcgcaatg ctgccgccat tgatggtttc
     1441 caaatggttt aagcagcgca ataaagtgga tttacccgag ccagatgagc caataatcgc
     1501 caccacttct gactggcgca cttgtaaatt cacatctttg agcacttcca acgcgccaaa
     1561 cgatttgcgg atgtgctgcg cgtctatcat caaatcagtc ttcgtagacg gcatagcgtt
     1621 tctccatgta ttggaacatc caagtcaaaa tcaaagtcat gatgaggtaa aaagcggcgg
     1681 cggtgagaaa aggggtgaca ttgaagtcgc gttgcacaat gttgcgggtg gcgcgcaata
     1741 agtcgttcaa agccaacaca tacaccaatg aagtgtcttt caccaaggta atggtttcat
     1801 tcgataaagg cggtaagatt tttttccaca tttgcggcaa cacaatgcgg cgcatggttt
     1861 gcggatacga catgcccaag gttttggcgg cttcgtattg accacgacct atcgccaaaa
     1921 tgccgccgcg aaagatttcg gcgaaataag cggcataatt caaggcaaat gccaccacgg
     1981 cggcagtgaa gtcttcaaaa taaatgccaa cagcgggcag ggcgaaataa acaaacagca
     2041 tttgcagcat cagcggtgtg ccgcgcatca gccagatata accgttaacg aagacatcca
     2101 aagccttgat gccagacagg cgcaaaagcg ccaaaatcag ccccagcggt atcgatagca
     2161 gcaaggtgat ggcaaacaac ttcaacgtca tttgcgcacc ttcccacatt gggggcagca
     2221 tttgt
//
LOCUS       DAOMVK010000377         2222 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_14410_length_2222_cov_3.086756, whole genome shotgun sequence.
ACCESSION   DAOMVK010000377 DAOMVK010000000
VERSION     DAOMVK010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14410_length_2222_cov_3.086756"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="PK856_10475"
     CDS             <1..1182
                     /locus_tag="PK856_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HRL02833.1"
                     /translation="NGVLGGPQIVVYEGDEVSIHFTNELEQETTVHWHGLPVPPEQDG
                     NPHDPVKPGQSLTYTFKLPQGCAGTYWYHTHAHELSAEQAFRGLAGTFIVKSHQDPMT
                     DWAEQHWLFSDLRLDKQGKIPDNTMMDWMNGREGQFVLLNGQLQPKISIKEATRIRVW
                     NVCNARYLKLHIPDCDIIVIGTDGGLLETPQAPVSELLIVPAERYEIVLKPRKTGQLT
                     LQNLPYNREKMMEEFKPVTTVLANIVYTASEKAVPAKLRTLPKLGKPTTEHTVGFSEE
                     MGAQMKQMFKVNGKTFDMQRIDLTSKLGATEDWLVSNASHMDHPFHLHGIQFEIIEFI
                     DSAESSRPAYRSLKDTVNLKPYQSVRVRCRQDFAGLRMFHCHILEHESLGMMGQVMVK
                     A"
     gene            complement(1419..>2222)
                     /locus_tag="PK856_10480"
     CDS             complement(1419..>2222)
                     /locus_tag="PK856_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRL02834.1"
                     /translation="LYQMQERGELPFPAINVNDSVTKSKFDNLYGCRESLVDGIKRAT
                     DVMIAGKIAIVAGYGDVGKGCAQSLRGLGATVWVTEIDPICALQAAMEGYRVVRMDEV
                     AGEADIFVTTTGNVNVITHDHMKAMRDQAIVCNIGHFDSEIEIASLRSYVWDNIKPQV
                     DHIEFPDGKRIILLAEGRLVNLGCGTGHPSFVMSNSFTNQVLAQIELFVRGADYQNEV
                     YVLPKHLDEMVARLHLERIGAKLTVLSDEQAAYINVPKEGPYKPNHYRY"
BASE COUNT          565 a          598 c          554 g          505 t
ORIGIN      
        1 aacggcgtgt tgggtggccc acaaatcgtg gtgtatgaag gcgatgaagt cagcatccat
       61 ttcaccaatg aattggagca agaaaccacc gtgcattggc atggtttgcc cgtgccgccc
      121 gagcaagatg gcaacccgca cgaccccgtg aaaccaggcc aaagcctcac ctataccttc
      181 aagctgccgc aaggctgcgc aggcacgtat tggtatcaca cccatgcaca cgagctttcg
      241 gccgaacaag ccttccgtgg cttggcgggc acgtttattg tgaaaagcca ccaagacccg
      301 atgaccgatt gggcggagca acattggctg ttcagcgatt tgcgcctaga caagcaaggc
      361 aagatacccg acaacaccat gatggattgg atgaacggcc gcgagggcca gtttgtgctg
      421 ctcaatggcc aattgcaacc gaaaatcagc atcaaagaag ccacgcgcat acgggtgtgg
      481 aatgtgtgca atgcgcgcta tttgaagctg cacattcccg attgcgacat catcgtcatc
      541 ggcaccgatg gcggcttgct cgaaacgccg caagcacctg tatcagagtt gctcatcgtg
      601 cctgctgagc gctatgaaat tgtgttaaag ccacgcaaaa cagggcaatt gacgctgcaa
      661 aacctgcctt acaaccgcga aaaaatgatg gaagaattca aacccgtcac cactgtgttg
      721 gccaacattg tttacactgc cagcgaaaaa gccgtgccag ccaagctgcg tactttgccg
      781 aaattaggca aaccaacgac agagcacacg gtgggtttca gcgaggaaat gggcgcacag
      841 atgaaacaga tgttcaaggt caatggcaag acgtttgaca tgcagcgcat agacctcacc
      901 agcaaactcg gtgcgactga agattggctg gtgagcaatg cctcgcacat ggaccacccg
      961 tttcatttgc atggcattca gtttgaaatc attgaattca tcgacagcgc cgaatcgagc
     1021 agacctgctt accgtagcct gaaagacacg gtgaatctca agccttacca aagcgtgcgc
     1081 gtgcgttgtc gccaagattt cgcgggcttg cgcatgttcc attgccacat attggaacac
     1141 gaatctttgg gcatgatggg gcaagtgatg gtcaaagctt aagcttagtc atgatgtgaa
     1201 tgttcaagca aagcgtgttg gcaacaacac gcttttttgt ttacaggccg ctgctgatgc
     1261 gcagtttgaa agtcaatgac ccaatctcca tatcatctta cacatgcggc ctgtaaaacc
     1321 attccatgat gtttaaacac aaaaaagcgt gccctaaggc acgctttatc cgtttacaga
     1381 ccgctataga gaacaatccc tagcagcagc gctgtgtctt agtaacggta atggttaggc
     1441 ttgtacgggc cttctttagg cacgttgatg taagccgctt gctcatcaga caacacggtc
     1501 aatttcgcac cgatgcgttc caaatgcaaa cgcgccacca tttcgtccaa atgcttaggc
     1561 aacacataca cttcgttttg gtaatccgcg ccgcgcacga acaactcaat ttgcgccaac
     1621 acttggttgg tgaagctgtt gctcatcacg aagctagggt gaccagtacc gcaacccaag
     1681 ttcaccaagc gaccttcggc caacaagatg atgcgttttc cgtctgggaa ttcgatgtgg
     1741 tcaacttgtg gtttgatgtt gtcccacaca tagctgcgca agctggcgat ttcgatttcg
     1801 ctgtcgaagt ggccaatgtt gcacacaatc gcttgatcgc gcatcgcttt catgtggtcg
     1861 tgggtaatca cgttcacgtt gcctgtggtg gtgacaaaaa tgtcggcttc gccggccact
     1921 tcgtccatgc gcaccacgcg gtatccttcc atagcggcct gtaacgcaca aatcgggtcg
     1981 atttcagtta cccaaacggt cgcgcccaaa ccgcgcaagc tttgtgcaca gcctttgccc
     2041 acgtcaccgt agcccgcaac aatcgcaatt ttgcccgcaa tcatcacgtc agtggcgcgt
     2101 ttgatgccgt ctaccaagct ctcgcggcag ccgtacaaat tgtcgaattt agacttggtt
     2161 accgagtcgt tcacattgat ggcagggaat ggcaattcgc cacgctcttg catttggtac
     2221 aa
//
LOCUS       DAOMVK010000378         2216 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_14463_length_2216_cov_2.757057, whole genome shotgun sequence.
ACCESSION   DAOMVK010000378 DAOMVK010000000
VERSION     DAOMVK010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14463_length_2216_cov_2.757057"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PK856_10485"
     CDS             complement(<1..509)
                     /locus_tag="PK856_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRL02835.1"
                     /translation="MGQIQDVFAKAAKAELWAGNLIAGRADSGKKLENRTPIDNSVIG
                     HLADGDAADIDAAVAVARQSFNSGEWSQLSPAERKAVMQRWAQLMQDNIEELAALDCV
                     DAGKPISECMNTDMPATIETFHWYAEAADKVFGKVAPTGPSALGLVVEEAIGVVGVVL
                     PWNFPAQMFA"
     gene            complement(520..1389)
                     /gene="dapA"
                     /locus_tag="PK856_10490"
     CDS             complement(520..1389)
                     /gene="dapA"
                     /locus_tag="PK856_10490"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRL02836.1"
                     /translation="MKIRGIYVPMVTPFDANNQIDVATLKSLTQTLIDSGVHGLVVCG
                     TTGEYYTFSDEERELVLSTVASIAKGKVALMAGINDLSTEGSAKKAQRAAELGYESLM
                     LAPPPYSLPSQEGVIAHFNKVAEATDLPIVMYNFPARTGISIEFDTVAELAKHPKIVG
                     IKESSGDFSRALSMLQADFADFTVICGCDDQPLDFFFWGAGAWIAGAANVFPAEQVAI
                     YNLTQAGKWDEAKKLMSQIYPVIHSMESGDYNQKAKLGCVKGSVKVGGVRTPLANLNE
                     ADKAEFLKLLAQA"
     gene            complement(1417..>2216)
                     /locus_tag="PK856_10495"
     CDS             complement(1417..>2216)
                     /locus_tag="PK856_10495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRL02837.1"
                     /translation="SVQGLQQHGSKLNGLGVSNTWVNAAALQEREPALAANQLGGLFF
                     PDTGHVTDLMAVLHKLAAEFQQAGGEIIENCAVLHAKQRGQQVVLNTTQGEKVVDKVV
                     VATGAHSKGLVAELTGCLVPLDTERGYHYMLPHEKNRLHLPVSSADRRFIMTPMEGGL
                     RLAGTVEYAGLQAAPNMERARQLLQVANPMLKQSLNSEGATEWMGFRPTIVDSLPVID
                     RFGSVFLAFGHQHLGLTQAAVTAQLMRSLLNSETPSLDLHPYRLNRF"
BASE COUNT          534 a          622 c          515 g          545 t
ORIGIN      
        1 gcaaacattt gtgcagggaa attccatggc aacaccacac ccaccacgcc tatggcttct
       61 tcaaccacca agcccaaagc cgaagggcct gttggtgcca ctttaccaaa cactttatca
      121 gcagcttccg cataccaatg gaaggtttcg atggtggctg gcatgtcggt attcatgcat
      181 tctgaaatcg gtttgcccgc atccacacaa tccaatgccg ccaactcttc aatgttgtct
      241 tgcatcaatt gtgcccagcg ctgcatcacg gccttgcgct cagcaggcga caattgtgac
      301 cactcaccgc tgttaaaact ttggcgcgct acggcaactg ccgcatcaat gtcagccgca
      361 tcgccatcag caaggtgacc aatcaccgag ttgtcgatgg gagtgcggtt ttccaatttt
      421 ttgccactgt cagcacgacc tgcaatcaaa ttgcctgccc acaattcggc cttggccgct
      481 ttggcaaaca catcttgaat ttgacccata tcttgctcct taagcttgtg ccaacaattt
      541 taagaattcg gctttgtcgg cttcattcaa gttagccaat ggggtgcgca cgccgcccac
      601 tttcacagag cctttcacac agcccaattt ggctttttgg ttgtaatcgc cagattccat
      661 agaatgaatc actgggtaga tttggctcat caattttttg gcttcgtccc atttgcccgc
      721 ttgggtcagg ttgtaaatcg ctacttgttc agctgggaac acgttggccg cacccgcaat
      781 ccaagcaccg gcgccccaga agaagaagtc taaaggttgg tcatcacaac cacagatgac
      841 ggtgaagtca gcaaagtcgg cctgtaacat agacaaggca cgactgaagt caccactgct
      901 ctctttgatg ccgacaattt tcgggtgttt cgccaattca gccacggtat caaattcaat
      961 gctgatgcct gtgcgcgctg ggaagttgta catcacgata ggcaagtcag tcgcttcggc
     1021 cactttgtta aagtgagcaa tcacgccttc ttggctaggc aagctgtatg gaggcggagc
     1081 caacatcagg ctttcgtagc ccaattcggc cgcacgttgg gcttttttcg ccgaaccttc
     1141 ggtagataaa tcattgatgc ccgccatcaa agccactttg cctttggcaa tggaagccac
     1201 agtagacaac accaattcgc gctcttcgtc gctgaaagtg taatactcac cagtggtacc
     1261 acacaccacc aagccatgta caccgctgtc tatcaaggtt tgggtcaaag atttcaaagt
     1321 ggccacatcg atttggttgt tggcatcgaa tggggtaacc ataggcacgt aaataccacg
     1381 aattttcatg ttctattcct ttatttgtgt gttgtttcaa aaacggttca aacggtatgg
     1441 atgcaaatcc aagctcggcg tttcactgtt taacagcgaa cgcatcaatt gcgcggtcac
     1501 tgccgcttgg gtcaagccca agtgctggtg accaaaagcc aaaaacacgc tgccaaagcg
     1561 gtcgatgaca ggcaaagaat cgacgatggt cgggcgaaaa cccatccatt cagtcgcgcc
     1621 ttcgctgttc aaagactgct tgagcatggg attggccact tgtaacaact gtctggcgcg
     1681 ctccatattg ggagcggcct gcaatcctgc atattccacc gtacctgcca aacgcaagcc
     1741 accttccatc ggtgtcatga tgaagcgtcg gtcggcactg gaaacaggca gatgcaaacg
     1801 gtttttttcg tgtggcaaca tgtagtggta accacgctcg gtatccaatg gcaccaaaca
     1861 accagtgagt tcagccacca agcctttgga atgggcaccg gttgccacca ccactttgtc
     1921 caccactttc tcgccttgtg tggtgttcaa caccacttgc tgaccgcgtt gtttcgcatg
     1981 cagcacggca cagttttcaa tgatttcacc accagcttgc tgaaactcag ccgccagctt
     2041 gtgtaaaacc gccatcaaat cggtgacatg gcccgtatcg gggaaaaaca aaccacccaa
     2101 ttggttggcc gccaaagcgg gttcgcgctc ttgcaaagcc gccgcgttca cccaagtatt
     2161 gctcacgccc aagccattga gcttgctgcc gtgttgttgc aaaccttgca cgctgg
//
LOCUS       DAOMVK010000379         2208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_14524_length_2208_cov_3.997213, whole genome shotgun sequence.
ACCESSION   DAOMVK010000379 DAOMVK010000000
VERSION     DAOMVK010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14524_length_2208_cov_3.997213"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..906)
                     /locus_tag="PK856_10500"
                     /pseudo
     CDS             complement(58..906)
                     /locus_tag="PK856_10500"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
     gene            complement(1131..1673)
                     /locus_tag="PK856_10505"
     CDS             complement(1131..1673)
                     /locus_tag="PK856_10505"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRL02838.1"
                     /translation="MTTPELDDAALEFASKVFAAAREGDADALKQWLDQGLPVNLRNH
                     NGDTLLMLASYHEHAKATQVLLERGADAQILNERGQSPLSGAAFKGNVEITQLLLNHG
                     AQVDVHGEDGRTPLMFAAMFNRTDIMEVLLREGGANILNKDAAGNDTIACAKALNAQE
                     SLAWLELYLAGDDKDFFELS"
     gene            complement(1802..>2208)
                     /locus_tag="PK856_10510"
     CDS             complement(1802..>2208)
                     /locus_tag="PK856_10510"
                     /inference="COORDINATES: protein motif:HMM:NF016843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HRL02839.1"
                     /translation="TVLLYLQAASQLTWLMASFGASCLIVFALPDSPLAQPRNVIGGH
                     VLSAATGIAMAHFVGVTPMSMALAVALAIVLMLWTRTPHPPAGANPIIVMMMGSSWSF
                     LLFPVLFGAVVLVGVACAMHKLNHQTYPKYWW"
BASE COUNT          673 a          584 c          437 g          514 t
ORIGIN      
        1 gttaatacgc ttagtttacc aagtttagtt gtacggtttc ttcagcatag ctcaatgcta
       61 caaatacaac gaataagaca cgttgccaca ccattgcaac caagattgtt gcagcaattt
      121 gttgtcacgt tgtgcggcca tcaccagcac acttgcaatc acaggcaaca gtgcccaata
      181 actgggccag gccaaagtac tgttgtatgc aaatactgcc atacccaata taagcaaacc
      241 caaatattgc aataccgttc gggctgatga tgacaggcga ttttgagagc caagtgtgta
      301 taacaatcca ccactgacca tttcccaagc cctggttggc aacataaaga atgcatattc
      361 aggataggta cgactcaata ccaaattaca cccaaacgaa gccaaccaca ccaccaccaa
      421 taccaaattc aaactgctca gcaatggctt acatttccag cccaaccaca ataaaacagg
      481 gtatataaga taaaactgcc attcaaccga caacgaccat gtgtgcaata cctatttttc
      541 atttgcatgg gcggcaaaat agccagactc ttggtaaaac gtattatttg aaacaaacag
      601 catggtctta tacacatgct tggccaatgt cacatagtct tgcggtactg gcaccaagac
      661 tgctgctgca cccaataaca ccacacacaa cacccacaaa gcaggaatga tgcgccaggc
      721 ccgagccaaa tagaatgtca ccagcgaaaa tcgctgttgt tgaaaggcct gtaccacaat
      781 agccgtcatc aaaaaacccg agatgacaaa aaacacatcc acacctacaa caccatcttg
      841 tagcttgaaa atgccaaagt ggtaagcaat cactgccaac accgcaccag cgcgcagtgc
      901 attaatatct gttctaaatc cttacattgc caatgctgtc ctcaattcaa ttcgcgtgca
      961 tcataacaca cgcccaattt aagcctgctc agatgcatag cctctgatga aaactgattg
     1021 acacacttca tttcaaccat acattttaaa accaccgcat cacaagcaaa agcggcctgt
     1081 aaatctttac aggccgcttt tatgcgcata acggaatctt aaaatagctt ttacgacaac
     1141 tcaaaaaaat ctttgtcgtc gccagccaaa tacaattcca accatgccaa tgactcttgg
     1201 gcgttcaagg ctttggcgca ggcgatggtg tcgttgcctg cggcgtcttt attcaagatg
     1261 tttgcgccac cctctcgcaa caaaacttcc atgatgtcgg tgcggttgaa catggcggca
     1321 aacatcaatg gcgtgcgtcc atcttcacca tgcacatcga cttgtgcgcc atggttgagc
     1381 agcaattggg taatttccac attgccttta aacgccgcac ctgacaaagg gctttgaccg
     1441 cgctcgttta aaatttgtgc atcagcaccg cgctctaaca acacttgcgt ggctttggcg
     1501 tgctcatggt aactggccaa catcaataag gtatcgccat tatgattgcg taaattaacc
     1561 ggcaaacctt ggtctagcca ctgcttcaaa gcatcggcat cgccttcgcg ggcggcggca
     1621 aacactttgc tggcaaattc taaggctgca tcgtctaatt caggggtagt cattcagagt
     1681 ctccataatt ttgtttattt taaactatca ttcaaagaca tagaattaat attaacaatg
     1741 taactataga cgtgtattca tacggctttg tgcagggttg aaatgagttt tggcagtgta
     1801 ttcaccacca gtacttggga taggtttggt ggttcaattt gtgcattgca caagccaccc
     1861 caaccaagac cactgctcca aacaacacag ggaaaagcaa aaatgaccac gagctgccca
     1921 tcatcatcac aataatcgga ttggcacctg caggcggatg tggtgtacgt gtccataaca
     1981 tcaacacaat ggccagcgcc accgccaatg ccatgctcat gggcgtcacg cccacaaaat
     2041 gcgccatcgc tatgcctgtg gcggctgaca agacatgtcc gccgatgaca ttgcgaggct
     2101 gcgccaacgg agagtcaggc agcgcaaaca ctatcaaaca gctggcacca aacgatgcca
     2161 tcaaccaagt gagctgagaa gcggcctgta aatacaataa aaccgtaa
//
LOCUS       DAOMVK010000380         2186 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_14721_length_2186_cov_2.902393, whole genome shotgun sequence.
ACCESSION   DAOMVK010000380 DAOMVK010000000
VERSION     DAOMVK010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14721_length_2186_cov_2.902393"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..369
                     /locus_tag="PK856_10515"
     CDS             22..369
                     /locus_tag="PK856_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRL02840.1"
                     /translation="MSIQRHITNQRLSEAVKANGFIFLAGQVPTNPEGDAEAQTANVL
                     AQIDTLLQDLGSDKSKILEATIYITDLASYEGLNRAWDAWVPAGHAPARACVKADLAN
                     PDWLVEIKLTALA"
     gene            553..1605
                     /locus_tag="PK856_10520"
     CDS             553..1605
                     /locus_tag="PK856_10520"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HRL02841.1"
                     /translation="MTTFAQLQLSRPIVQAPMAGGPSSPQLVAAVSNAGGLGSFACGM
                     ISPQQMREGVSQIRSLTSKPFAMNLFVLPESVQPDAAALSSSKKWLEPALRQVGLSMP
                     TPTSWSQSFQAQFAALLELAPTVASFAFGILNATQVAALKQRNILVVGTANSVEHLQA
                     WEAVGADAIVVQGLEAGGHRGGPTELRLEQELNLDDLLAACRPLTELPLIAAGGIMDA
                     ERIQALLAAGADAVQMGTVFLCSDESGASSVMKQALWQRRDQATRLTRLFSGKPARGV
                     VNAWMAQLAEHESDTLGYPYHNALTTPLRTWANQANEPDYLALWAGTGLAQMQKTSAI
                     NILDALYPIVVSESTS"
     gene            complement(1703..>2186)
                     /locus_tag="PK856_10525"
     CDS             complement(1703..>2186)
                     /locus_tag="PK856_10525"
                     /inference="COORDINATES: protein motif:HMM:NF021430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2135 domain-containing protein"
                     /protein_id="HRL02842.1"
                     /translation="QLIAVDELNQLVAAHKNHINTAQFDAALLHNRPVDMRVVLTWDS
                     DNSDMDLWVTDPNGEKVFYGHQQSYQGGKISRDFTGGYGPEVFWLKDPKAGEYEIRAH
                     FYGDRQQIVTGPTTAHVRLIRHWGKPNQSEQILRVKMAQEREGQSNQESVLIGTFTVQ
                     "
BASE COUNT          511 a          621 c          532 g          522 t
ORIGIN      
        1 cctattgtaa ggattatcac catgagcata caacgtcaca tcaccaatca acgtttgtct
       61 gaagcagtca aagccaatgg attcatcttc ttggctggcc aagtgcccac caatcctgaa
      121 ggcgatgcag aagcgcaaac cgccaatgta ttggcacaaa tagacacttt gttgcaagac
      181 ttgggctctg acaaatccaa aatccttgaa gccaccattt acatcaccga tttggccagc
      241 tacgaaggct tgaaccgcgc ttgggacgct tgggtacccg caggccatgc gcctgctcgc
      301 gcttgcgtca aagccgactt ggccaaccct gattggttgg ttgaaatcaa attgactgca
      361 ttggcctaaa ccatgtgtat gaaaaagcgt cctgtaaacc atttacaggg cgcttttttg
      421 ttgccgaatc ttcattgatg ctcttgtttg atataacact tgcagcgccc aaagtgagaa
      481 gcagccatct gtacaaggct ggccacatat tgcatgataa gcatgaagcc tcatccattt
      541 taaggagcgt gtatgaccac attcgcacaa ttgcaattaa gccgccccat cgtccaagcc
      601 cccatggcgg gcgggccatc gtcaccacaa ttggttgccg cagtcagcaa tgcaggcggt
      661 ttgggcagtt ttgcctgcgg catgatttca ccgcagcaaa tgcgtgaagg cgtgagccaa
      721 atccgcagcc tcacatccaa acccttcgcc atgaatttgt ttgtgttacc cgaatcggta
      781 cagcccgatg ccgcggcctt aagctccagc aaaaaatggc tggaacctgc gctgcgccaa
      841 gtcggcttga gcatgcccac acccacttct tggtcgcaat cgttccaagc tcagtttgcc
      901 gccttattgg aactcgcgcc caccgttgcc agctttgcct ttggcatttt gaacgccact
      961 caagtggccg cattgaaaca acgcaacatc ttggtagtcg gcacggccaa cagtgtggaa
     1021 catttgcaag catgggaagc agtcggtgcc gatgcgattg tggtgcaagg cttggaagca
     1081 ggcggtcacc gcggcggccc caccgaattg cgtttggaac aagagttgaa tctggatgat
     1141 ttactggccg catgccgccc cttaaccgag ttgccattga ttgccgcagg cggcatcatg
     1201 gatgctgagc gcatccaagc cttgcttgcc gcaggcgccg atgcagtgca aatgggcacg
     1261 gtgtttttgt gcagcgatga aagtggcgcg tccagcgtga tgaaacaagc cttgtggcaa
     1321 cgccgtgacc aagccacccg cttaacccgc ttgttttcgg gtaaacccgc tcgcggcgtg
     1381 gtcaatgctt ggatggcaca actggccgag cacgaaagcg acaccctcgg ctacccttac
     1441 cacaatgcct taaccacacc actgcgcact tgggccaatc aagccaacga accagattac
     1501 ttggctttgt gggcaggcac aggtttggca cagatgcaaa aaaccagtgc cattaacatt
     1561 ctcgatgcct tataccccat cgtggtgtcg gaatccacct catgagccca tacccacaca
     1621 tgcggcctgt aaagttacag gccgcttttt tattgattta aattaatccg tgtagtgcgc
     1681 tggcaagcca tgccttaccc actcactgca ccgtaaacgt accgattaac accgactctt
     1741 gattgctttg tccttcacgc tcttgcgcca ttttcacccg caagatttgc tcgctttggt
     1801 tgggcttgcc ccaatggcgt atcaagcgca catgggctgt ggtcgggcct gtgacgattt
     1861 gctgtctgtc accgtaaaaa tgggcacgaa tctcatactc gcccgctttc gggtctttca
     1921 accaaaacac ttcggggcca tagccgcccg tgaaatcgcg cgaaatcttg ccgccttggt
     1981 aactttgttg gtggccataa aacacttttt cgccattggg gtcggtcacc cacaaatcca
     2041 tgtcagaatt gtcgctgtcc caagtgagca ccacccgcat gtctacaggc cgattgtgta
     2101 ataaggccgc atcaaattgg gcggtattga tgtgattttt gtgcgccgcc accaattggt
     2161 ttaactcatc caccgcaatc aactgt
//
LOCUS       DAOMVK010000381         2182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_14768_length_2182_cov_3.231312, whole genome shotgun sequence.
ACCESSION   DAOMVK010000381 DAOMVK010000000
VERSION     DAOMVK010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14768_length_2182_cov_3.231312"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /gene="yidC"
                     /locus_tag="PK856_10530"
     CDS             <1..999
                     /gene="yidC"
                     /locus_tag="PK856_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRL02843.1"
                     /translation="EGKYQKVDFKSLDEDFQNGKDTADYQRKTESGWVGMIQHYFVTS
                     WILNKKDAASVCGKAENCTMDVKKRSDGLYSAGVRVTLPDLATGQTTAVPMTLFAGPA
                     EYNRIMPLADNLELSKDFGKLHIFASPLFKLLNWIHGYVGNWGWAIILLTIIVKAVLF
                     PLTNASYKSMAKMRAVAPRLNALKAEYGDDRMKMQQEMMALYKKEKINPLGGCLPMLL
                     QIPVFLGLYWALFNSVELRQAPWMGWIQDLARPDPWFILPVLMAATMWLQTHLNPPPA
                     DPMQAKMMKIMPLMFSVMFFFFPAGLVLYYVVNNVLSIAQQWFINKKIEKSMKTAA"
     gene            1126..2121
                     /gene="dusB"
                     /locus_tag="PK856_10535"
                     /pseudo
     CDS             1126..2121
                     /gene="dusB"
                     /locus_tag="PK856_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958170.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
BASE COUNT          565 a          495 c          571 g          551 t
ORIGIN      
        1 gaaggcaaat accaaaaagt ggatttcaaa tctttggatg aagacttcca aaatggcaaa
       61 gacaccgctg actaccaacg caagactgaa tctggttggg tgggcatgat tcagcattac
      121 ttcgtcactt cttggatttt gaacaagaaa gatgctgcca gcgtgtgtgg caaagctgaa
      181 aactgcacca tggatgtgaa aaaacgcagc gatggtttgt attcagccgg tgtgcgtgtg
      241 accttgcctg atttggcaac gggtcaaacc actgccgtgc ccatgacttt gtttgcaggc
      301 cctgcggaat acaaccgcat catgcctttg gcggacaatt tggaattgag caaagacttc
      361 ggtaaattgc acattttcgc gtcgccattg ttcaaattgt tgaactggat tcatggttat
      421 gtgggcaact ggggttgggc aattattttg ctcaccatca ttgtgaaagc ggtgttgttc
      481 ccattgacca atgcgtctta caaatcgatg gccaaaatgc gtgcagtcgc accgcgtttg
      541 aatgcgctca aagccgaata cggcgacgac cgcatgaaaa tgcagcaaga aatgatggct
      601 ttgtacaaga aagagaagat caatccattg ggcggttgtt taccgatgtt gcttcaaatt
      661 cctgtgttct tgggcttgta ctgggcattg ttcaactcgg ttgaattgcg tcaagcgccg
      721 tggatgggtt ggattcaaga cttggcccgc cctgaccctt ggttcatctt gccagtgttg
      781 atggcagcga ccatgtggtt gcaaacccat ttgaatccgc cgccagcaga ccctatgcaa
      841 gcgaaaatga tgaaaatcat gccgttgatg ttctcggtga tgttcttctt cttccctgca
      901 ggtttggtgc tttactatgt ggtcaacaac gtgttgtcga ttgcacagca atggttcatc
      961 aacaagaaaa ttgaaaaaag catgaagacc gccgcataag cacgcagtca acatgaaata
     1021 agcggcctgt aagttacagg ccgcttattt tttacttgga aatgcttccg ataactgctt
     1081 aaaaaatgta caattgcctc cctgtttgat ttgatgatga gacgcatgca cataggcagt
     1141 attgagttgg ccacgccttt ggcattggcg cctatggctg gcatcactga caaaccgttt
     1201 cgtatgttgt gtcgccgctt tggtgcaggg ctggccagca gtgaaatgac cacttccgac
     1261 cctagtttgc aaaacacccg caaaaccttg cagcgcgccg atcacagcgg tgaaacaggc
     1321 atacgttcgg tgcaaattgt tggcaacatt cctgaggtga tggcggaagc ggccaagctg
     1381 aatgtggcgc gtggcgcaca aatcatcgac atcaacatgg gttgcccagc caaaaaagtg
     1441 tgtaatgtgt tggcaggcag tgctttgtta aaagacgaag ccttggtgca agacatttta
     1501 caggccgtgg tacaaagcgt ggatgtgcct gttaccttga aaacccgttt gggttgggac
     1561 gatgcccact tgaatgtgcc caccattgcc aaaatagccg tggatgcagg catacagact
     1621 ttggcgattc acgggcgcag ccgcacacaa atgtacacag gtgacgcacg ttacgaattg
     1681 attgcgcagg tcaagcgcga attgccgata ccgctgtggg tgaacggtga cattaccact
     1741 gccgccaaag ccttgcaggt attggagcaa acaggcgccg atggcgcgat gataggtcga
     1801 ggcgcacaag gccaaccgtg gatttttcgc gacatcaagc acatgcttga gcatggcgtg
     1861 ttaccagaag ccttggcctt tcaagaggcc gccagtgtgg tgttagagca tttggcggcc
     1921 atacatgttt tttatggcga acatggggtg aaaatcgccc gcaagcattt gttatggtat
     1981 tagggcgggt ttgatgccta tcaacgtgag cgcaccgaaa tcaatcaaat acaagaaact
     2041 tcattacaat acgaaaaaat cgctgattta ttagaacaat tgtcacatca gtatgatttg
     2101 tggcctcgac gtgttgccta gctcggcagc gcgacgtgcc cctcatttta ggagcaactt
     2161 aatgaatgaa cgcatcgata tc
//
LOCUS       DAOMVK010000382         2179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_14804_length_2179_cov_1.844162, whole genome shotgun sequence.
ACCESSION   DAOMVK010000382 DAOMVK010000000
VERSION     DAOMVK010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14804_length_2179_cov_1.844162"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(564..1511)
                     /locus_tag="PK856_10540"
     CDS             complement(564..1511)
                     /locus_tag="PK856_10540"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRL02844.1"
                     /translation="MKNKPQISDLDVFCVVVESQSFVGAATELGVSPAFISKRIQILE
                     KTLDCKLLNRSTRNISLTEDGKYIYEQSRRILVDLEYMTEALSSKNDMLAGNIHVTCG
                     FSLGRQYIAPLLSQLLENHPKLKLQLNTLDTRQNLLRSNIDLDIRVGNDLDPNVIARR
                     LAPSIRILCAAPSYIERKGVPESLDDLHNHQCLFIKETDYPLGTWKLENRNKEHTVKL
                     RPHLSSNNSEIIKLWTLQGHGIMMHSLWDVLDYLKQGELLHVMPDYWERADIFGVYPA
                     RLTQSSKVKACLDYLEEELVGMLPLEEIEAITQYSYDPY"
     gene            1647..>2179
                     /locus_tag="PK856_10545"
     CDS             1647..>2179
                     /locus_tag="PK856_10545"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRL02845.1"
                     /translation="MNKVYKIAAIPGDGIGIEVLPEGLKVLQAAADKHHIALEITQFD
                     WASCDYYLEHGNMLPDNWFEVLKNFDAIYFGAVGMPDKVPDHISLWGSLLQFRRRFDQ
                     YVNLRPVRLLPGVPCPLAGREPGDIDFYVVRENTEGEYTSVGGRMFEGTDREFVLQES
                     VFTRHGSDRILKYAFEMA"
BASE COUNT          564 a          514 c          505 g          596 t
ORIGIN      
        1 caccttgtgc ttcaaattgg cattggccct tatacagctg ccgcccacct tcttcgattt
       61 ggcaaacagc ggctttaggt gccgccccaa cccacgcgct gcttatcaac acaccgcaag
      121 ccaagataaa tgccgctgat tttgtgttca ttacactctc cctgtatcaa ctcattgcag
      181 tttaaagccc caagcagaca ggttgtgtcc agtttatgca aataaaataa ccattgctgt
      241 caaatagaga caagtctgaa tgaatgcagc aagcattggc attgctcagt gctgaaaagt
      301 cgtgctggct ttgaacgaat attgattgca tgaatggcgg tgtttgatgg tgaaattgaa
      361 gcagattaaa gttattttga cggacatcga aaaagtaaac atgaacaaag tactgttgga
      421 aaaaacgata aaattgacac gcctcttagc gtttagcact tctcatccac cactgattta
      481 caggtgtaca caccaccgca tatacataaa agcggcctgt aaaccttttt aaaaggttta
      541 caggccgctt tatttcgcaa cactcaataa gggtcgtaac tgtattgggt aatcgcttcg
      601 atttcttcta aaggcaacat gcccacgagt tcttcttcta agtaatccaa acaggctttg
      661 actttggagg attgggttaa acgcgcggga tacacgccaa aaatatcggc acgttcccaa
      721 taatcgggca tgacatgcaa caattcgcct tgcttcaaat aatccaatac atcccacaag
      781 gagtgcatca tgatgccgtg gccttgcagc gtccacagtt tgatgatttc gctgttgtta
      841 gacgacaagt gcgggcgcaa tttgacggtg tgttctttgt tgcggttttc cagcttccac
      901 gtgcccaaag gatagtcggt ttccttgatg aacagacatt ggtggttgtg caaatcgtcc
      961 aagctctcag gcacaccttt gcgctctatg taactgggcg ccgcgcacaa gatgcgtatg
     1021 ctcggagcca atcgtctggc gatgacattg gggtctaagt cattgcccac acggatatcc
     1081 aaatcaatat tgcttctgag caggttttgt cgcgtgtcca aagtgttcaa ttgcagcttg
     1141 agtttggggt gattttcaag caattgcgac agtaaaggcg cgatgtattg tcgccccaag
     1201 ctgaagccgc aggtgacgtg aatattgccc gccagcatgt cgttcttgct cgacaaggct
     1261 tcggtcatgt attccaaatc caccaaaatg cggcgcgatt gctcgtaaat gtatttgccg
     1321 tcttcagtga ggctgatgtt gcgggtgctg cggttgagca atttacagtc tagggttttc
     1381 tctaagattt ggatgcgctt gctgatgaat gcaggtgaaa cgcccaattc ggtagcggcg
     1441 cccacaaagc tttggctctc gaccaccaca caaaaaacgt cgagatcaga aatctgcggc
     1501 ttattcttca cgattcgtgt cctttgtatt catgtttctc atcattattt taaatccgat
     1561 tcgcggtatt ctatatttgc gctaacgaag tgggtattca aaatacttac tgtgaatata
     1621 aatataaaaa aggacgccat aaacccatga acaaagtcta caaaattgct gccattcctg
     1681 gtgatggcat cggtatagaa gtcttaccag agggcttgaa ggttttacag gccgctgcag
     1741 acaaacacca cattgctctg gaaatcacac aattcgattg ggcgagttgc gattactacc
     1801 ttgagcacgg aaacatgcta cccgataact ggtttgaagt gttgaaaaac ttcgatgcca
     1861 tctattttgg cgccgtaggc atgcctgaca aggttcctga ccacatttct ttatggggct
     1921 cattgctaca attccgccgc cgcttcgacc aatatgtgaa tttgcgccca gtacgcttac
     1981 tgcctggcgt gccttgcccg ttggctggcc gtgaaccagg cgacattgat ttttatgtgg
     2041 tgcgcgaaaa cactgaaggc gaatacacct cagttggcgg ccgcatgttt gaaggcacag
     2101 accgcgaatt tgttttgcaa gagtcggtgt tcacccgcca cggctcagac cgcatcttga
     2161 aatacgcttt tgaaatggc
//
LOCUS       DAOMVK010000383         2178 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_14811_length_2178_cov_2.365520, whole genome shotgun sequence.
ACCESSION   DAOMVK010000383 DAOMVK010000000
VERSION     DAOMVK010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14811_length_2178_cov_2.365520"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1817)
                     /locus_tag="PK856_10550"
     CDS             complement(<1..1817)
                     /locus_tag="PK856_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02846.1"
                     /translation="MNDFTSIYAPIAATGKLDGHPEAVIVALKDLFASLEAIRKDVPG
                     LAMDYVLNGDSAQVLHDLGTGKAGRSFEYLATPGSIVDYYHSVNSKSDTRVLNARVRL
                     YQLLDKPDIKPEQWQRLAQVFAASYPDPKHDYYAAASFASSVPDWLQVLLADIVITMS
                     KRVKFGTTLVGPQAHLTKSFFDRMVSLASMSVPAETVFFERDALKNSWHTDNLEKFWR
                     LKDFDTEWITPELTLALAASLSNSGRLALLTFLGSDEQRLVRYSQTVVKLGLMAGKKL
                     QGDARLLLSRLPLENLLPHINAVLLGEGSNAERKLAAEMLIPFGVAAEPTLQAALKVE
                     NNAAVKQVYESVLSRLQTVSGMAEQDNSFDVPAWEPLVTGKIDEAEVVAILQQSHADI
                     LKAAQESAAAEAKENEDPAYKGYKSVWASKRLKELNKIDAAVFPRIAAALNGGEAVSR
                     KLVDYQLINHKSRIEAVAGFDFGHFLRLMSLTHGGGNNWWQIREYLNKAFPQGIDLRV
                     MADAIIKNGRNEKAAYVQIADEYLGWMGSDAMEGRDADKVWPFFYEHPEFLQEAFGLT
                     KNKTSTSYGGYQQGNAVQVLAMFPKIPSEWLPILLTLAL"
BASE COUNT          558 a          599 c          480 g          541 t
ORIGIN      
        1 tccaaagcca aggtcaacaa gatgggcaac cattcgctcg ggattttcgg gaacatcgcc
       61 aacacttgca ccgcattgcc ttgttggtag ccaccgtatg aagtactggt tttgttttta
      121 gtcagcccaa acgcttcttg caagaactca ggatgctcat agaaaaacgg ccacacctta
      181 tcggcatcac ggccctccat tgcatcactg cccatccagc ccaaatactc atcggcaatt
      241 tgcacataag cggctttttc attgcggcca tttttgatga tggcatccgc catcacacgc
      301 aagtctatgc cttgcggaaa ggctttgttc aggtattcgc ggatttgcca ccaattatta
      361 ccgccaccat gagtgaggct cattaaacgc aagaaatggc caaaatcaaa acctgccacc
      421 gcttcaatgc ggcttttgtg gttgattaac tggtaatcca caagtttgcg cgacaccgct
      481 tcgccaccat tgagggcagc ggcaatgcgc ggaaacactg ccgcatcgat tttgttcaat
      541 tcttttaagc gtttgcttgc ccaaacggat ttgtaacctt tgtaagcggg gtcttcgttt
      601 tccttggctt cggcagcggc cgattcttga gcggctttca aaatatcggc gtggctttgc
      661 tgcaaaatcg ccaccacttc ggcctcgtca atcttgcccg ttaccaaagg ctcccatgca
      721 ggcacatcaa aactgttgtc ttgctctgcc atgccactga cggtttgcaa acgcgacaac
      781 acactttcat acacctgctt caccgcagca ttgttttcca ctttcaatgc ggcctgtaag
      841 gtcggctctg ctgccacgcc aaacggtatc agcatttctg ccgccagttt gcgttcggca
      901 ttgctgccct cgcccagcaa taccgcattg atgtgaggca acaggttttc caaaggcagg
      961 cgtgacagca acaagcgcgc atcaccttgc agttttttcc ctgccatcaa gcccagtttc
     1021 accacggttt ggctgtaacg caccaaacgc tgctcatccg aacccaaaaa tgttaacaag
     1081 gccaaacgcc cagaattgct caggctggct gccagagcca gagtcaattc tggcgtgatc
     1141 cactccgtgt caaagtcttt taagcgccag aatttttcca aattgtcggt atgccagctg
     1201 tttttcaaag catcgcgctc gaagaacacc gtttcggcag gcacactcat gcttgccaaa
     1261 gacaccatgc ggtcgaaaaa tgatttggtc aaatgagctt gcggcccaac gagagtcgtg
     1321 ccgaatttca cccgcttgct catggtaatc acaatgtcgg ccaacaacac ctgcagccaa
     1381 tcaggcacgc tggaagcaaa cgaggcggct gcataataat cgtgtttggg gtcgggataa
     1441 ctggcggcaa acacctgcgc caagcgctgc cactgctcag gcttgatatc gggtttgtcc
     1501 aacaattggt agagtcgcac tcgcgcattc aacacccgcg tatcagactt gctgttgacg
     1561 ctgtggtagt aatcgacaat agagcctggc gtggccaaat actcaaaact gcgccccgct
     1621 ttgcctgtgc ccaaatcatg caagacctgt gcagagtcac cgtttaacac atagtccatg
     1681 gccaaacctg gcacatcttt gcgtatggct tccaaacttg cgaacaagtc tttcaaagcc
     1741 acaatcacgg cttcgggatg gccatccaat tttccagtgg cagcgatggg cgcataaatg
     1801 gaggtgaaat cattcatgtt aggtccttag ataacagtgg catttaaatc aatgggataa
     1861 ttgggctgtg ctgacaacac ttacaggccg ctcattcagt tctagatact atgctcagca
     1921 acataaccga aatcgcccgt tttggcgata tgttcacggc tgatttaacc aaaaaaaacg
     1981 ctgtgatagc cttgtcatca agcatttcac cctgtaaaaa tcccaatatg aatatcgtta
     2041 cacatttctt gtaacagtaa tggaaagcag actgcaaggt caaccctatt ttcggccgca
     2101 ataaaaaaac acctgtgtgc cccacaggtg ttttaaaaca atcaataaac gttttacagg
     2161 ccgcttaatg ccacaaat
//
LOCUS       DAOMVK010000384         2165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_14943_length_2165_cov_2.490047, whole genome shotgun sequence.
ACCESSION   DAOMVK010000384 DAOMVK010000000
VERSION     DAOMVK010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14943_length_2165_cov_2.490047"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /gene="fabD"
                     /locus_tag="PK856_10555"
     CDS             complement(<1..864)
                     /gene="fabD"
                     /locus_tag="PK856_10555"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HRL02847.1"
                     /translation="MAFAFFFPGQGSQSLHMMDGFNDISVVRHTFQEASDVLGEDLWS
                     MINGDDAAKLNATVITQPLMLTAGVATWRAYQHLGGVRPAALAGHSLGEYTALVAAQS
                     LDFATAIKLVSERAHLMQNAVPQGVGAMAAILGLSDADVMDVCRLAQDGGEVVEAVNF
                     NAVGQVVIAGNKAAVETAMALAKEKGAKRALPLPVSVPSHCSLMKPAAEKLKTYLEGV
                     NIVAPQIPVIHNVDVNTHQDVALIKDALVRQLYSPVLWTQTVNKLVEDGITEAAECGP
                     GKVLAGLSKRIN"
     gene            complement(888..2081)
                     /locus_tag="PK856_10560"
     CDS             complement(888..2081)
                     /locus_tag="PK856_10560"
                     /inference="COORDINATES: protein motif:HMM:NF012534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock and DUF1294 domain-containing
                     protein"
                     /protein_id="HRL02848.1"
                     /translation="MAQYQGQISDWQKAKGFGFITPQGGGKRIFFHLNAWLGNRQNIQ
                     TQQVVKYDLTTDTKGRICALNVVPIANLIDGMQLQAVLVRYLPEKKLALLSHADYPNT
                     QFVLPQAALQTDMPTIGSNISGVLKLHSSGHWLMMQAQVQDTTAAQKSIVKNMASAAP
                     QCMPESDSKAHQTSTRAQAQSLANGQQAQAKHWYDGMRLEGLLQDWNDDKGYGFVVVD
                     GHSKQVFFHINDYIELNGRPKNGMKMRFNAKQEGGRWRAEAVQCLSAPAAIPKNRRHR
                     GQLQGEPIKIIVAVLLALLFLAYISHYNKAVALWMLVVSVVSFVTYRTDKQAALKNTW
                     RVPENKLHFLDLIGGWPGGLIARQLWRHKTTKTSFVVTFWLTVIINIIASNWLMRYAA
                     NYLPM"
BASE COUNT          528 a          602 c          484 g          551 t
ORIGIN      
        1 gttaatgcgt ttggacaagc cagccaacac cttgcctgga ccacattcag ctgcttcggt
       61 gatgccgtct tctaccaatt tattgaccgt ttgtgtccaa agtactggtg aatacaattg
      121 gcgcaccaac gcgtctttaa tcaaagccac gtcttggtgg gtgttcacgt ccacgttgtg
      181 tatcacagga atctgtggcg caacaatgtt cacgccttcc aaatacgttt tgagtttttc
      241 cgccgctggc ttcatcaagc tgcagtgcga aggcacagac acaggcaaag gcaaggcacg
      301 ttttgcgcct ttttctttgg ccaaagccat ggcggtttcc acagcggctt tgttacccgc
      361 aatcaccact tgacccacgg cattgaaatt caccgcttcc accacttcac caccgtcttg
      421 cgccaagcga cagacatcca tcacgtctgc atcgctcaag cccaaaattg ccgccatcgc
      481 acccacgcct tgcggcacgg cattttgcat cagatgtgcg cgttcgctca ccagtttaat
      541 cgcagtggca aaatccaagc tttgcgccgc caccaaagcg gtgtactcgc ccaagctatg
      601 gcctgccaaa gccgcaggcc ttacaccgcc caagtgttgg taagcgcgcc aagtggctac
      661 acctgccgtc aacatcaaag gctgggtaat caccgtggca ttgagtttgg cggcatcgtc
      721 gccattgatc atggaccaca agtcttcacc caacacatcg gaagcttctt ggaaggtatg
      781 acgcaccacg ctgatgtcgt taaaaccatc catcatgtgc aaggattgtg aaccttgtcc
      841 aggaaagaaa aatgcaaaag ccattgttgt cctctcttca attctgttta catcggcaaa
      901 taatttgccg catagcgcat cagccaatta ctggcgatga tgttaataat cacggtgagc
      961 caaaaagtga ccacaaaact ggtttttgtg gttttgtgtc gccacaattg tctggcaatc
     1021 agacctccag gccagccacc aatcaaatct aaaaaatgca atttgttttc aggcacccgc
     1081 caagtattct ttaaggccgc ttgtttatcg gtgcggtaag tcacaaaaga caccacactc
     1141 accaccagca tccacagggc cacggccttg ttatagtggc tgatgtaagc caaaaacagc
     1201 aaagccaaca acaccgccac aatgattttg atgggctcac cttgcaattg tccgcgatga
     1261 cggcggtttt tagggattgc cgcaggcgct gataaacact gaaccgcttc cgcgcgccaa
     1321 cggccaccct cttgcttggc attaaagcgc atcttcatgc catttttggg ccgaccattg
     1381 agttcaatgt aatcgttgat gtggaaaaaa acctgttttg aatgaccatc aaccaccaca
     1441 aaaccatagc ctttgtcatc gttccaatcc tgaagcagac cttccaaacg catgccatcg
     1501 taccaatgct tggcttgggc ttgctgtcca tttgccaagg actgggcttg cgctcgtgta
     1561 ctggtttgat gcgctttgga atcagattca ggcatgcact gaggcgcagc cgaagccatg
     1621 tttttcacaa tgctcttctg tgcggcagtg gtgtcttgca cttgcgcttg catcatcagc
     1681 caatggccgc tgctgtgcag cttcagcacg ccgctgatgt tgctgccaat ggtcggcatg
     1741 tcggtttgta aggctgcttg tggcaaaaca aattgggtat tggggtaatc ggcatggctt
     1801 aaaagagcca gttttttctc aggtaaatac cgcaccaaaa cggcctgtaa ctgcattccg
     1861 tctatcaaat tggcgatggg taccacattc aaagcgcaga tgcggccttt ggtatcggtg
     1921 gttaaatcgt atttcaccac ttgctgggtt tgaatgtttt ggcggttacc cagccacgca
     1981 ttcaagtgaa aaaaaatccg ttttccccct ccttgtggtg taataaaacc aaaccctttt
     2041 gccttttgcc aatcactgat ttggccttgg tattgtgcca agtttccccc ttatcaacgc
     2101 ccaatcaaaa tccatcaata acgcaataac accgcgcccc atgcaaagcc gccgcctatg
     2161 ccttc
//
LOCUS       DAOMVK010000385         2164 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_14952_length_2164_cov_2.541963, whole genome shotgun sequence.
ACCESSION   DAOMVK010000385 DAOMVK010000000
VERSION     DAOMVK010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14952_length_2164_cov_2.541963"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1332)
                     /locus_tag="PK856_10565"
     CDS             complement(<1..1332)
                     /locus_tag="PK856_10565"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRL02849.1"
                     /translation="MSITFADLKLNDTLVKTITNAGYVSPTPVQMQSIPAAIEGHDLL
                     VSAQTGSGKTAAFLLPALEKLQARSDKPGKGPRVLVLTPTRELAQQIEKNAQIYGENM
                     KWLRTVTLVGGTSFGYQTRALNRPIDIIVATPGRLMDHMRQGRIDFSRLEMLILDEAD
                     RMLDMGFVDDIETIVAATPDDRQTLLFSATLDGIVGNIARKITNEPQSIEIERVQEES
                     RIEEQLIYSDDFRHKDRLLDHILRDANIDQCVIFTATKAFTEQLADQLYEKGYAAECL
                     HGDMPQSWRNRTLNNLRTGRTKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRSGIAITFAEVKEYMKVSKIERYLKRQLPEAEIPGLEATRRKPKGGAGRSG
                     SGAGRRGGSNGGYGDKRRSFDGRDGNRSEGSGRSYASRDDNRGNREERSTDS"
     gene            1664..>2164
                     /locus_tag="PK856_10570"
     CDS             1664..>2164
                     /locus_tag="PK856_10570"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRL02850.1"
                     /translation="MSEITAFANRLGKNAKHLIKWARRQNIEAFRLYDRDIPQYPLII
                     DVYGDRVHLQEFDTGWLLHAEEHQAWLNEAIEAICFVTGFQPEDVAVKVRKRQKGEEQ
                     YEKTGREGDDFTVQENGRLFWVNLDKYLDTGLFLDHRNTRLVVGDMVAGKRFLNLFAY
                     TGSFSVY"
BASE COUNT          512 a          501 c          570 g          581 t
ORIGIN      
        1 gctgtcagtg ctgcgttctt cacggttgcc acggttgtcg tcacgtgagg cgtaagaacg
       61 gccactgcct tcgctgcggt tgccatcacg gccatcgaat gagcggcgtt tgtcaccgta
      121 accaccgttg ctgccaccac gacgacctgc acccgaacca ctgcggccag cgccaccttt
      181 gggtttgcga cgggttgctt ccaagcctgg aatttcagct tctggcaatt ggcgtttcaa
      241 gtagcgctca attttgctga ctttcatgta ctctttcact tcagcaaacg tgatggcgat
      301 accgctgcga ccggcacggc cagtacggcc aatgcggtgt acatagtctt cggcttgttt
      361 tggcaagtcg tagttgatga cgtgggtaat ggtaggcacg tcgataccac gcgcagccac
      421 gtcggttgcc accaagattt tggtgcgacc cgtacgcaag ttgttcaaag tgcggttacg
      481 ccagctttgt ggcatgtcgc catgcaaaca ttctgctgcg tagccttttt cgtacaattg
      541 gtcagccaat tgttcggtaa atgctttggt tgccgtgaaa atcacacatt ggtcgatgtt
      601 ggcatcgcgc aagatgtggt ctaacaaacg gtctttgtga cggaagtcgt ctgagtaaat
      661 caattgttct tcgatgcggc tttcttcttg cacgcgttcg atttcaatgc tttgtggctc
      721 attggtgatt ttgcgcgcaa tattgccgac gatgccgtcc aaggttgcag agaacaacaa
      781 ggtttgacgg tcgtcagggg tggcagcaac gatggtttcg atgtcgtcaa caaagcccat
      841 gtccagcatg cggtcggctt cgtccaaaat caacatttcc aaacgtgaaa agtcgatgcg
      901 gccttggcgc atgtggtcca tcaggcggcc tggagtggcc acgatgatgt caatcggacg
      961 gttcaaagcg cgggtttggt aaccgaaaga agtgccgcct accaaagtca cggtgcgcaa
     1021 ccatttcatg ttttcgccgt agatttgcgc gtttttctcg atttgttgtg ccaattctcg
     1081 ggtaggagtc aataccaaca cgcgtgggcc tttaccaggc ttgtcgctgc gtgcttgtaa
     1141 tttttccaaa gcaggcaaca aaaacgctgc ggttttacca ctgcctgttt gggcagatac
     1201 caacaagtca tggccttcaa tggcggctgg gatagactgc atttgcacgg gggttggaga
     1261 cacgtagcct gcgttggtga tggttttaac cagagtatcg ttaagtttta aatctgcaaa
     1321 agtgatggac ataccattcc tgtaatgaaa acagcaaaca acaagcaata catgagcgaa
     1381 tgcccttacc gcgtggtatt ttgccgaaaa gccctgtggc tttggtttac gaatgtcggc
     1441 gacaaccaat cggttgggat ttcagcttgc tcgaaagtct gatttttcaa agaaaatcac
     1501 gagagaatag caggcagagg ttgaagacga cagcttcaat ttgaaagtag tcgagcataa
     1561 taatgcataa atgaatttga tgcaagtgtt tgcgcacaat ttatgcgtgt acatggtttt
     1621 tttcggtaca atgagcgcat tcttgttgtg aaattgatat cccatgtctg aaattaccgc
     1681 gtttgccaac cgcttgggca aaaatgccaa acacctgatc aaatgggcgc gccgccaaaa
     1741 tatcgaagcg tttcgtttgt acgacagaga catcccgcaa tacccattaa tcatcgatgt
     1801 gtatggcgac cgtgtgcatt tgcaagaatt cgacacaggt tggcttttgc atgccgaaga
     1861 gcatcaggct tggcttaacg aggccatcga agcgatttgc tttgtcacgg gttttcagcc
     1921 tgaagacgtg gctgtgaaag tgcgcaagcg tcaaaaaggc gaagagcaat acgaaaaaac
     1981 tggccgcgaa ggtgatgatt ttactgtgca ggaaaatggc cgtttgtttt gggtcaacct
     2041 cgacaaatac ttagacacag gtttgtttct cgatcaccgc aatacccgat tagttgtcgg
     2101 tgacatggtc gcgggcaaac gctttttgaa tttgtttgct tacacaggca gcttttctgt
     2161 gtat
//
LOCUS       DAOMVK010000386         2160 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_14990_length_2160_cov_2.219952, whole genome shotgun sequence.
ACCESSION   DAOMVK010000386 DAOMVK010000000
VERSION     DAOMVK010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14990_length_2160_cov_2.219952"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="PK856_10575"
     CDS             complement(<1..471)
                     /locus_tag="PK856_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02851.1"
                     /translation="MKKVLINVSIIVGMLLLYFVPSILMGLSMLSASEHVGESFVWFR
                     DLAPFLGMLIASIGLLYWAHTLGWFENLNWRIGGKGWLHLIVVFVLMQAWVMAFSQFK
                     PEHTENDQAIMELMDLFPLWLMFVFICIVAPICEEVIFRGAIIGQAFAKHLWWGA"
     gene            944..1834
                     /locus_tag="PK856_10580"
     CDS             944..1834
                     /locus_tag="PK856_10580"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRL02852.1"
                     /translation="MPRTQIRAEMMLALATLGWGMNYPLMKMALQHELGIWTLAVRFS
                     LSFLIMLPLFWMQRQHLSWGSAKIGIVLGLILLPSVELMNLGLHYTSSANAGFIFALC
                     IIWVPILNAYLNKTRISPQIKFSMTLGLIGLSIIANLHQLKFNYGDFLILLCSFLYAA
                     YILVLDRYGKTYSGIVMTAMQLLVLGVGCTVIYAIAKPPVHEINWSWQLVAIILASAI
                     VSSTFATWIQTRYQADTTPDRAALIFNLEPVFATIFATWWLHEAVGLNIIVGGSLILA
                     AIIVPTLAKRLFDNKPRQST"
BASE COUNT          614 a          583 c          422 g          541 t
ORIGIN      
        1 cgcaccccac cacaaatgct tggcaaacgc ctgcccgatt atggccccac gaaaaatcac
       61 ttcctcacaa atgggcgcga caatgcagat aaacacaaac atcagccaca aaggaaacaa
      121 atccatcaat tccatgatgg cctggtcgtt ctccgtgtgc tcaggcttaa attgactgaa
      181 ggccatcacc cacgcctgca tcaacacaaa caccacaatc aggtgtagcc agcctttgcc
      241 accgatgcgc caattcaaat tctcaaacca acccagcgtg tgcgcccagt acagcaaacc
      301 gatgctcgca atcaacatgc ccaagaatgg cgccaaatcg cgaaaccaca caaagctctc
      361 acccacgtgc tcactcgccg acagcatgga caaacccatc agtatcgacg gcacaaaata
      421 cagcaacaac atgccaacga tgatggaaac attgatgagg acttttttca tagcggcttt
      481 caagtgcggg cggaagaaaa tacaaacggt tttattgggt cacaaactca gctgagaata
      541 ccgacaaggc ttgcaatact caagcccaag cctgtttaca gaccgcttat ggcatcttaa
      601 aaagcggcct gtaaacacaa ttaaagcact ggcatgcctt atcaaggcaa tgaataaacc
      661 atgataacgg tcacgctata caccatttgt ggcctgcttc agacacaatc catcgcatgc
      721 ttgcgcccac aacccatctt tcagagcaaa cctgtctcac ataaacaatt gatttcactg
      781 cattgcaaaa cagcgttttc cagccaacaa agccgccctc gataagtaaa aaaacaatct
      841 aaaatttaga tgacaccaaa gcacacaaat cgcgtttctg tcgctacaat gaattcacac
      901 ctcgtcttta aaaccttaca aaacaatcca aagttgactt cccatgccac gcactcaaat
      961 acgcgctgaa atgatgcttg cccttgccac cctaggttgg ggcatgaatt accctctgat
     1021 gaaaatggct ttacaacatg aattgggcat ctggaccttg gccgttcgtt tcagcctcag
     1081 cttcctcatc atgttgccgc ttttttggat gcaacgccaa cacctctctt ggggcagcgc
     1141 caaaatcggc attgttttgg ggctgatttt attgccatcg gttgaattga tgaatttggg
     1201 cttgcactac accagctccg ccaacgcagg ctttattttc gcactgtgca tcatttgggt
     1261 accgatttta aatgcctacc tgaacaaaac ccgcatcagc ccgcaaatca aattcagcat
     1321 gaccttaggc ctcattggct tgtccatcat tgccaatttg caccaattga aattcaatta
     1381 cggcgacttt ttgattttgc tgtgctcatt tttgtatgcc gcgtacatct tggtgctcga
     1441 ccgctatggc aaaacctatt caggcattgt gatgacggcg atgcaattgt tggtattggg
     1501 cgtgggctgt acggtgattt acgccatcgc caagccgcca gtgcatgaaa tcaattggtc
     1561 atggcaattg gtggccatca ttttggcctc tgccattgtg tcatccacat ttgctacatg
     1621 gatacaaacg cgctaccaag ccgacaccac accagaccgt gctgctttga ttttcaactt
     1681 agagccggtt tttgccacca tctttgccac ttggtggctg cacgaggcag taggcttgaa
     1741 catcatcgtc ggcggcagtt tgattttggc cgccattatt gtcccaacct tggccaaacg
     1801 attgttcgat aacaaacccc gccaatcgac ttgaatccta gctcaggccg cttaacaagc
     1861 tgacccagtt gaaaagcaaa acaagcatcg tgttttggct acgtgaaaat ttgattcggt
     1921 ttgcagtgtt accaaagcaa aacccacaca aaacacaccg catttaagtg tttgaaatcg
     1981 catgcatatg gttttcagtc tcacaaatgt gtctctttta aacactgctc atgaggcaat
     2041 ttgctgcgtc ttgccaatta aatgggtaaa atccacaaat caacttgaaa taacagccgt
     2101 tttcatgata taaatctcgt ttttactaat cggtaatttg gagtctaacc atggcacgag
//
LOCUS       DAOMVK010000387         2159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_14996_length_2159_cov_2.844582, whole genome shotgun sequence.
ACCESSION   DAOMVK010000387 DAOMVK010000000
VERSION     DAOMVK010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14996_length_2159_cov_2.844582"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="PK856_10585"
     CDS             complement(<1..968)
                     /locus_tag="PK856_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRL02853.1"
                     /translation="MNLVFGVLLCLPIVATLLPLLRSSHWTVRIFDFPRLQIAFLSLI
                     FIILSLVSTNEPWWMVGLQIINALCLCYQLYKISAYGPWHRKQVLDAHGPDDERTISI
                     LNSNVLTPNRHYQQVLDMVGRYQPDLFLSLESDAWWEEKLKPLEQEYPYTVKIPLDNL
                     YGMHLYSRLELSDTEIRYWVREDIPSIVSDVVLRSGEKIKIYCLHPEPPTPGESDVST
                     PRDAELLLAGKEIDRSDETVIVFGDLNDVAWSHTTHLFQKISGLLDPRIGRGLFSTFH
                     AHHWFLRWPLDHMFVSNDFLVKKLLRLGDVGSDHFPVYAKFQYHPKA"
     gene            complement(1312..>2159)
                     /locus_tag="PK856_10590"
     CDS             complement(1312..>2159)
                     /locus_tag="PK856_10590"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HRL02854.1"
                     /translation="QLLGMNCNTPTDIVFSFEGFRLRGGLTDSHADGFGIAFFEGKGV
                     RLYHDDKPSASSPVADLIKAYQIKSENVIAHIRKATQGEVNLANTHPFVRELWGEYWV
                     FAHNGNLCDFHPAVGEFYRPVGNTDSERAFCFILEQLRQRFSHKPEQAVLMEHIASLV
                     KTIRGHGLFNVLMSNGDLMFAHASTLLHYIVRKAPFGEAVLLDDDINIDFAAVTTPND
                     RVAVIATLPLTTNEQWQQLAVNELVCFQAGVVVFSDAPEPKQYMGIEEGLCIARKVGA
                     AGRID"
BASE COUNT          584 a          493 c          539 g          543 t
ORIGIN      
        1 gctttcggat gatactgaaa tttagcataa acggggaagt ggtcggaacc cacatcaccc
       61 aaacggagca gttttttgac caaaaagtca ttgctgacga acatgtggtc tagcggccag
      121 cgcaaaaacc aatggtgggc atgaaaggta ctgaataagc cccgaccgat tcgagggtct
      181 agcaagccgc tgattttttg aaacaaatgg gtggtgtgtg accaggcaac atcgtttaaa
      241 tcgccaaata caatcacggt ttcatcactg cggtcaattt ctttgcccgc caacagcaat
      301 tcggcgtcgc gtggtgtcga aacatcactt tcaccaggag taggaggttc tgggtgtaga
      361 cagtaaattt tgattttctc acccgagcgc aaaaccacat cggaaacgat ggaaggaatg
      421 tcctctctta cccagtaacg tatttcggtg tcacttaatt ctaggcggct gtataagtgc
      481 atgccataca aattgtccag tgggattttt acggtgtatg ggtattcttg ttccaaaggt
      541 tttagctttt cttcccacca cgcatcgctt tctaagctta ggaataaatc aggttggtag
      601 cgaccgacca tgtccaatac ctgctggtaa tggcgattgg gggttaagac attgctgttg
      661 aggatggaga tggtgcgctc atcatcaggc ccatgggcat ctagcacctg tttgcgatgc
      721 catgggccat aagcgctgat tttgtacagc tggtagcaca gacacaaggc attgatgatt
      781 tgtaagccca ccatccacca aggttcgttg gtgctgacca gactgagaat gatgaatatc
      841 aggctgagaa atgcgatttg taagcgcgga aagtcaaaaa tgcgcaccgt ccaatggctg
      901 ctgcgcaaca aaggcagtaa ggtggcaacg atggggaggc acagtaacac accaaaaacc
      961 aaattcatgc atggcctttc aatacttggc ggtcatgcgt tgattcacac acagccaagg
     1021 tgtacaaaga ttgggaatac atgtggataa tcataacaag tgtttggtac caaaaggggt
     1081 ttttcaattg cttaaatttt gggcagtgct gccataagcg gcctgtaaac acatcaaaaa
     1141 ccatttatgc acatctcgtc cacacattta tcccaatttt gccatgtcgc agcacccgat
     1201 tcagattgtc tgtgtaacta tggacaacag gcgggggcaa ggagcgggat atcatgtgga
     1261 taaggtattc aagttcatga tttttattga taaaaaacga ttgattaaaa attaatcaat
     1321 tcttcctgca gctcccactt tgcgagcgat gcacaaaccc tcttcgatac ccatgtattg
     1381 tttgggctcg ggtgcgtcac tgaacacaac cacacctgcc tgaaaacaca ccaattcgtt
     1441 gacggccaat tgttgccatt gttcattggt ggtgaggggg agggtggcaa tcacggccac
     1501 gcggtcgttt ggtgtcgtga ctgccgcaaa atcaatgttg atgtcgtcgt cgagcaatac
     1561 cgcttcgcca aacggggctt tgcgcacaat gtaatgcaat aaggtgctgg catgggcaaa
     1621 catcaggtcg ccgttcgaca tcaacacatt gaacaagcca tgaccacgta tggttttcac
     1681 caggctcgca atgtgttcca tcaacactgc ttgttcgggt ttgtggctaa agcgttggcg
     1741 caattgttct aagatgaagc aaaaggcgcg ttctgagtcg gtattaccca caggtcggta
     1801 aaattcaccc acagcagggt gaaagtcgca caaattacca ttgtgggcga atacccaata
     1861 ttccccccac aattcgcgca caaatggatg ggtgttggca aggttgacct cgccttgggt
     1921 ggccttgcgg atgtgggcaa tcacgttttc ggatttgatt tgataggctt tgatcaaatc
     1981 ggccactgga gacgaagcac tgggtttgtc atcatggtac aaacgcacgc ctttgccttc
     2041 aaaaaaggcg atgccaaagc catctgcatg gctgtcggtt aagccaccac gcaagcgaaa
     2101 cccttcaaac gaaaaaacga tgtcggtggg cgtgttacaa ttcatgccca ataattggc
//
LOCUS       DAOMVK010000388         2158 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_15006_length_2158_cov_3.939135, whole genome shotgun sequence.
ACCESSION   DAOMVK010000388 DAOMVK010000000
VERSION     DAOMVK010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15006_length_2158_cov_3.939135"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1469)
                     /locus_tag="PK856_10595"
     CDS             complement(<1..1469)
                     /locus_tag="PK856_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HRL02855.1"
                     /translation="MAHPPLRGASLVWVTLALSIAVFMQVLDSTIANVALPTISGNLG
                     AATSQGTWVITSFAVANAISVPLTGWLAKRFGEVKTFVTSTALFVLASWLCGIAPSLE
                     VLILFRVMQGAVAGPMIPLSQSLLLAAYPPEKRPMALALWSMTIIVAPIFGPILGGII
                     SDNWHWGWIFFINIPIGVFAVWVAYKQLHHRETEIKHQPIDYLGLSLLVLGVGALQMM
                     LDRGKELDWFASTEIIVLCVIAVVSLAYLIVWELGSEYPIVDLKLFKDRNFTVGVVSL
                     SLAFMVYMGAIVLLPLVMQTQLGYTATWAGLAAAPIGILPVILSPLIGKFGGRMDMRI
                     LVTMSFLTYAACFWWRTDFNADMDFWDIFWPQFWQGVGMSMFFLPLTQITLSRIAPEN
                     IANASSLSNFIRILAGGIGTSIATTLWERREAVHHAHLVESINPYNPMSVEMQQQMGQ
                     MGMSVTQQHAVLNQTITQQAYIMASNEVFYAGAILFIVL"
     gene            complement(1479..>2158)
                     /locus_tag="PK856_10600"
     CDS             complement(1479..>2158)
                     /locus_tag="PK856_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRL02856.1"
                     /translation="AVEAAQAGLTAAHAQEQQSKAALGRESLREQPAVQTAMSHMKDA
                     WLNLERTHIKAASDGQVARRNVQVGQKVAAGSPMMAIVPLNNLWVDANFKENQLSKLR
                     LGQPVSLVSDLYGDKVVFHGKVLGLSAGTGSAFSVLPAQNATGNWIKVVQRVPVRIQL
                     DAKELAEHPLRIGLSMKATVDISNQQGAAVATATVKDKTLATAAATDRFTEVDAAINS
                     ILSSYEH"
BASE COUNT          551 a          618 c          545 g          444 t
ORIGIN      
        1 attaacacga tgaacaaaat cgcccccgcg taaaacacct cattcgaggc catgatatag
       61 gcttgctggg taatggtttg gttcaatacc gcgtgttgct gggtaacact catgcccatt
      121 tgccccattt gctgctgcat ttccacgctc atggggttgt atgggttaat cgactccacc
      181 aaatgcgcat ggtgcacggc ttcacgccgc tcccacaaag ttgtcgcgat ggatgtgccg
      241 atgccaccgg ccaaaatgcg gatgaaattg gacaagctcg acgcattggc aatgttttca
      301 ggggcgatgc gcgacaaggt gatttgggtc aaaggcagga agaacatcga catgcccacg
      361 ccttgccaaa attgcggcca gaaaatgtcc caaaaatcca tgtcggcatt gaaatcggtg
      421 cgccaccaaa aacacgcggc ataggtaaga aaactcatgg ttaccaatat gcgcatgtcc
      481 atgcgcccac caaatttacc aatcagcggc gacagaatta ccggcaagat gccgatgggt
      541 gcggcggcca agcctgccca agtggcggta tagcccaatt gggtttgcat caccaaaggc
      601 aagagcacaa tcgcgcccat gtacaccatg aaagccaaac tcaaagacac cacacccacg
      661 gtgaaattgc ggtctttgaa cagtttcaaa tcgacaatcg ggtattccga acccaattcc
      721 cacacgatca aatacgccaa gctcaccacc gcaatcacac acaacacaat gatttcggta
      781 gaggcaaacc aatccagctc tttgcctcga tcgagcatca tttgcagcgc acccacgccc
      841 aaaaccaata aggacaagcc caaatagtct atgggttggt gtttgatttc ggtttcgcgg
      901 tggtgcaatt gcttgtaagc cacccacacc gcaaacacgc ctatcggaat gttgatgaag
      961 aaaatccagc cccaatgcca gttgtcactg atgatgccac ccaaaatcgg cccaaaaatc
     1021 ggcgcaacaa tgatggtcat cgaccacaaa gccagagcca tcgggcgttt ttcaggcgga
     1081 taagcggcca gtaacagcga ttgcgacaaa gggatcatcg gccctgccac cgcgccttgc
     1141 atcacccgaa acaaaatcaa cacttccaag ctcggggcga tgccacacag ccacgaggcc
     1201 aacacaaaca aggctgtaga ggtaacaaag gttttcacct caccaaaacg cttggcgagc
     1261 caaccggtta aaggaacaga aatcgcgttc gccaccgcaa acgaggtaat cacccacgtg
     1321 ccttgcgacg tggccgcgcc caaattgcct gaaatcgttg gcaaggccac gttggcaatg
     1381 gtggaatcca acacctgcat gaacacggcg atggacaagg ccagcgtgac ccaaacgagg
     1441 ctcgcgcctc ggagtggagg atgtgccatc aagccgcctt aatgttcgta tgaactcaaa
     1501 atgctgttga tggcggcgtc cacttcggta aagcggtctg tcgcggcggc agtggccaag
     1561 gttttgtctt tgacagtggc ggtggccacg gctgcgccct gctgattgga aatgtctacc
     1621 gtggctttca tcgacaaacc gatgcgcagc ggatgctcgg ccaattcttt ggcatccagt
     1681 tgtatgcgca caggcacacg ttgcaccact ttaatccagt tgcctgtggc attttgcgct
     1741 ggcaacacac tgaaggcgct gcccgtaccg gctgacaaac ccaagacttt gccgtggaac
     1801 accactttgt cgccatacaa atcagacacc aagcttacag gctggcccaa gcgcaatttc
     1861 gacaattggt tttctttgaa attggcgtct acccacagat tgttcagtgg cacgatggcc
     1921 atcatcggac tgcctgcggc cactttttga cccacttgca cattgcgacg cgccacttgg
     1981 ccatcactgg cggctttgat gtgggtgcgc tccagattga gccaagcatc tttcatgtgt
     2041 gacatcgcgg tttgcacggc tggctgctcg cgcaaagatt cgcggcccaa agcggctttg
     2101 gattgctgct cttgtgcgtg tgcggcggtc aagccggctt gcgcggcctc cacagccg
//
LOCUS       DAOMVK010000389         2148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_15120_length_2148_cov_3.040612, whole genome shotgun sequence.
ACCESSION   DAOMVK010000389 DAOMVK010000000
VERSION     DAOMVK010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15120_length_2148_cov_3.040612"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="PK856_10605"
     CDS             <1..432
                     /locus_tag="PK856_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRL02857.1"
                     /translation="IAILSILQATLTLPGIAAIALTLGMAIDSNVLINERIREELRAG
                     HSPQVSINKGYEHAWATIVDSNITSLIAGIALLVFGSGPVRGFAVVHCIGILTSMFSS
                     VVCSRALVNLWYGRRPRLKSVSIGHVWRPDAAQQTAVAEKE"
     gene            436..1371
                     /gene="secF"
                     /locus_tag="PK856_10610"
     CDS             436..1371
                     /gene="secF"
                     /locus_tag="PK856_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HRL02858.1"
                     /translation="MELFRIKRDIPFMSYGKLTTAISLITFILAVFFLVTKGLNFSVE
                     FTGGTVMQVQYQQGADVNKIRSDLNTLKLGEVQVQAMGTTRDIMIRLPNKGSENAAAL
                     SDKVLETLKKSDPNVKLLQNDFIGPQVGEELITHGLAAIAMVCIGIIIYLAIRFEWRF
                     AVSAIIANMHDVVIILGCFALFQWEFSLTVLAGVLAVLGYSVNESVVVFDRIRENFRK
                     PQMRDHTIPEVIDNAITQTMSRTIITHGSTEAMVLSMLIFGGAALHGFAMALTIGIVF
                     GVYSSVLVASPLLMFFGLNRQNLVKERKRKEEAVV"
     gene            1428..2084
                     /locus_tag="PK856_10615"
     CDS             1428..2084
                     /locus_tag="PK856_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HRL02859.1"
                     /translation="MEFFSFIIDFILHIDQHLTELAAQYGVWIYGILFLIIFCETGLV
                     VMPLLPGDSLLFAAGSIAAIGKMNIHLMVVLLIIAAILGDAVNFMVGKFFGEKLFANP
                     NSKIFKQSHLQKTQQFYAKHGGKTIILARFIPIVRTFAPFVAGMGHMTYHHFLAYNVI
                     GGVLWVTIFSYLGYFFGNMDIVKDNLSLVLVAIIVLSILPAIIEIIRHKRAANKAGAQ
                     "
BASE COUNT          498 a          532 c          527 g          591 t
ORIGIN      
        1 attgccatct tgtcgatttt acaggccact ttgaccttgc ctggtatcgc agccattgcc
       61 ttaacattgg gtatggcgat tgactccaac gtattgatta acgagcgcat ccgcgaggaa
      121 ttgcgtgcgg gccacagccc tcaagtgtcc atcaacaaag gttacgagca tgcttgggcg
      181 acgattgttg actccaacat cacttcgttg attgcgggca ttgctttgtt ggtatttggc
      241 tctggcccag tacgcggctt tgctgtggtg cactgtatcg gcattttgac ctcaatgttc
      301 tcatcagtgg tgtgttcgcg cgcactggta aacctgtggt atggccgtcg ccctcgcttg
      361 aaatcggtgt ctattggcca tgtgtggcgc ccagatgcgg cccaacaaac cgcagtagca
      421 gaaaaggaat aagccatgga attgtttaga atcaagcgcg atattccgtt catgagttat
      481 ggcaagctca ccactgccat ttcattgatt accttcattc ttgcagtgtt tttcttggtg
      541 accaaaggcc tgaatttttc ggttgagttt acaggcggca cggtgatgca agtgcaatac
      601 cagcaaggtg cggatgtgaa caaaatccgc agtgacctca acacgctgaa attgggtgag
      661 gtgcaagtgc aagccatggg caccacccgt gacatcatga tccgcctgcc taacaaaggc
      721 tcggaaaatg cagcagcctt gtctgacaaa gtgttggaaa ccctgaaaaa atcagacccc
      781 aacgtgaagt tattgcaaaa cgacttcatc ggcccgcaag taggtgaaga gctgattacc
      841 cacggtttgg cggccattgc catggtgtgt atcggcatca tcatttactt ggccatccgt
      901 tttgaatggc gttttgccgt gtcggccatc attgccaaca tgcatgacgt ggtaatcatc
      961 ttgggctgtt ttgccttgtt ccaatgggaa ttttcattga ctgtattggc aggcgtgttg
     1021 gcggtactcg gttactcggt gaacgaatcg gtggtggtat tcgaccgtat tcgtgaaaac
     1081 ttccgcaagc cgcaaatgcg cgaccacacc ataccagaag tcatcgataa cgcgattacc
     1141 caaaccatga gccgtaccat catcacccac ggctcgaccg aggcgatggt cttgtcgatg
     1201 ttgatattcg gcggtgcggc tttacacgga tttgcaatgg ctttgaccat tggtatcgtg
     1261 tttggcgtgt attcttccgt tttggttgcc agtccattgt tgatgttctt cggcctcaat
     1321 cgccagaatc tggtgaaaga gcgtaagaga aaagaagaag cagtggttta acacaaacaa
     1381 gcggcctgta agtttacagg ccgctttcac atacacctcg agtcatcatg gaatttttca
     1441 gttttatcat cgattttatt ttgcacatag accaacacct caccgagctt gcagcgcaat
     1501 acggcgtgtg gatttatggc attttgtttt taatcatttt ctgcgaaaca ggtttggtgg
     1561 taatgccttt gctaccaggg gactctttgt tgtttgccgc aggcagcatc gccgccatag
     1621 gcaaaatgaa catccatttg atggtggtct tgctcatcat cgccgcgatt ttgggcgatg
     1681 cggtcaactt catggtaggc aagttttttg gcgagaaatt gttcgccaat cccaactcca
     1741 aaatcttcaa acaaagccat ttacaaaaaa cccaacagtt ttacgccaaa cacggcggca
     1801 aaaccatcat cttggcgcgc ttcattccca tcgtgcgcac cttcgccccg tttgtagcgg
     1861 gcatgggtca catgacttac caccacttcc tcgcttacaa cgtgatcggc ggcgtgttgt
     1921 gggtgaccat cttctcgtat ttgggttatt tctttggcaa tatggacatc gtcaaagaca
     1981 atttaagctt ggttttggtg gccatcatcg tgttgtcgat tttgccagcc atcattgaaa
     2041 tcattcgcca caaacgcgcg gccaacaaag ccggcgcaca gtaaagaccc cccgttatga
     2101 gcgcacgcat tcttgccctg cccgaccatt tggtcaatca aatcgctg
//
LOCUS       DAOMVK010000390         2143 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_15165_length_2143_cov_3.262931, whole genome shotgun sequence.
ACCESSION   DAOMVK010000390 DAOMVK010000000
VERSION     DAOMVK010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15165_length_2143_cov_3.262931"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PK856_10620"
     CDS             <1..183
                     /locus_tag="PK856_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HRL02860.1"
                     /translation="WLHRYGYWALLMSWVPFIGDGLAIAAGWLRLNVYLSTLMLVIGK
                     FARYGVLIFGTLQIIA"
     gene            227..>610
                     /locus_tag="PK856_10625"
     CDS             227..>610
                     /locus_tag="PK856_10625"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HRL02861.1"
                     /translation="MAEHKARKRFGQNFLQDTRIINDIVEAVRPQPDDVVVEIGPGLA
                     AITEPLARNLKQLHVVEIDRDIIARLKRLPFASKLVIHEGDVLQLDFRTISGMKKIVG
                     NLPYNISTPLLFELVEVADEVVDMHF"
     assembly_gap    611..620
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <621..1059
                     /locus_tag="PK856_10630"
     CDS             <621..1059
                     /locus_tag="PK856_10630"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HRL02862.1"
                     /translation="DHAEAIADMHFMLQKEVVERMVAEPKTNDYGRLSVVLQYYFEME
                     KILDVPPESFDPAPKVDSAVVRMIPNSGRIGVADDFKHLSRLLQHAFQQRRKTLRNNL
                     KEWVSDEDFAEVGIAPTLRAEHVAPEQYVQLSNHLLRKSVAID"
     gene            1087..1815
                     /locus_tag="PK856_10635"
     CDS             1087..1815
                     /locus_tag="PK856_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRL02863.1"
                     /translation="MIRFENVHKWFKDLHVINGVNLDIAKGEVVVVCGPSGSGKSTLI
                     RTVNQLETIQDGNIYVDGVGVNDHKTDLNKLRAEVGFVFQSFNLYPHLTAIENIMLSP
                     MKVKKMGAAEAKTLGMSLLKKVGLEHKAESFPGQLSGGQQQRVAIARSLAMEPKVMLF
                     DEPTSALDPEMVGEVLKVMKDLAKSGMTMMCVTHEMGFAREVADRVVFVDQGEIIEAA
                     TPEEFFTNPKHDRAKQFLSQLRAH"
     gene            1891..>2143
                     /locus_tag="PK856_10640"
     CDS             1891..>2143
                     /locus_tag="PK856_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HRL02864.1"
                     /translation="MAVDHYENFPVGSVLVPRRLRKPIHAIYAFARTADDYADEGGAD
                     NATRLAQLQALSDELVLIEQGKRPVTALMQRLQDEAIAPF"
BASE COUNT          544 a          453 c          597 g          539 t
ORIGIN      
        1 tggctgcacc gatatggcta ttgggccttg ctcatgtcgt gggtgccatt tattggcgat
       61 ggattggcca ttgcggcagg ttggttgcga ttgaatgtgt atttaagcac tttaatgttg
      121 gtcatcggca aatttgcgcg ttacggcgtg ctcatttttg gtacactgca aatcattgca
      181 tgaaatgcgg cctgtaaaac ggatacatgc cgcctagaag gaagttatgg cagaacacaa
      241 ggcccgcaag cgttttgggc agaatttttt acaagacaca cgcattatta atgacattgt
      301 tgaggccgtg cgtccgcagc ccgacgatgt ggtggttgaa attggcccag gcttggccgc
      361 aattaccgag cctttggccc gcaatttgaa acaattgcac gtggttgaaa tcgaccgtga
      421 tatcattgcg cgcttaaagc gcttgccgtt tgcgagcaaa ttggtgatac atgaaggcga
      481 tgtgttgcaa ttggattttc gcaccatctc tggcatgaaa aagattgttg gcaacctgcc
      541 ttacaatatt tcgacgccgc tgttgtttga attggtggaa gtggccgatg aagtggttga
      601 catgcatttc nnnnnnnnnn ggaccatgcc gaggcgatcg ccgacatgca cttcatgctg
      661 cagaaggaag tggtggagcg catggtggcc gagccgaaaa ccaatgatta cggccgcttg
      721 agtgtggtat tgcagtatta tttcgagatg gaaaaaatcc tcgatgtgcc acccgagtcg
      781 tttgaccctg caccgaaagt ggattctgct gtggtgcgca tgataccgaa ttcaggccgc
      841 atcggagttg cagatgattt caaacatttg agtcgtttgc tgcaacacgc atttcaacaa
      901 cgccgtaaaa ccttacgcaa taatttaaaa gagtgggtga gtgatgagga ttttgccgaa
      961 gtgggtattg cgcctacttt gcgcgccgaa cacgttgctc cagaacaata cgtacaattg
     1021 agtaaccacc tgctgcgcaa aagcgtggca attgattgat gacagggatg agccatggcc
     1081 aatgaaatga ttcgttttga aaatgtacac aaatggttta aagatttaca cgtcatcaat
     1141 ggtgtgaatc tggacatcgc caaaggcgaa gtggtggtgg tgtgtggacc gtctggttcg
     1201 ggtaaatcga ccttaatccg caccgtaaac caattggaaa ccatccaaga cggcaatatt
     1261 tacgtggatg gcgtgggcgt gaatgaccac aaaaccgatt tgaacaaatt gcgtgctgaa
     1321 gtgggtttcg tgtttcaaag cttcaatttg tacccacacc ttaccgccat cgaaaacatc
     1381 atgttgtcac cgatgaaggt caagaaaatg ggcgcggctg aagcgaaaac cttgggcatg
     1441 agcttgttga aaaaagtcgg tttggaacac aaagcagaat cgttccctgg tcaattgtca
     1501 ggcggtcagc aacagcgtgt ggcgattgcc cgcagcttgg cgatggagcc aaaagtgatg
     1561 ttgtttgacg aaccgacttc ggcactcgac cctgaaatgg tgggcgaagt gttgaaagtg
     1621 atgaaagact tggccaaaag cggcatgacc atgatgtgtg tgacccatga aatgggtttt
     1681 gcacgcgaag tggcagaccg cgtggtgttt gtggaccaag gtgaaatcat tgaggcggcc
     1741 acgcctgaag aattcttcac caatccaaaa cacgaccgtg ccaaacaatt cttgagccaa
     1801 ttgcgcgcgc actgataagc aagggttaaa tagcgtcctg taaacttaca ggacgctgtt
     1861 gttttttaaa cggatgcaca aggagtggct gtggcggtag accattacga aaatttccca
     1921 gtgggctcgg tgttggtgcc acggcgtttg cgtaaaccca tccatgcgat ttatgcgttt
     1981 gcgcgcaccg ccgatgatta tgcggatgag ggtggggcag acaatgccac gcgtttggca
     2041 caattgcagg ccttgtccga tgaattggtt ttgattgagc aaggcaaacg gcctgtaacg
     2101 gctttgatgc agcgtttgca agatgaagcg attgcgcctt ttg
//
LOCUS       DAOMVK010000391         2142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_15176_length_2142_cov_3.618112, whole genome shotgun sequence.
ACCESSION   DAOMVK010000391 DAOMVK010000000
VERSION     DAOMVK010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15176_length_2142_cov_3.618112"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="PK856_10645"
     CDS             complement(<1..909)
                     /locus_tag="PK856_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HRL02865.1"
                     /translation="MQQRPATSSIKSNPFMPQADLSQFSAVWPHALYALRDTLSNQQY
                     QMWIAPLTVSTENDAWVVYAKNNFVFGFVKSHFWQKIETAVANLAPNAPVLQLKIGMG
                     EQVSVASAPAATTPSAWVESEDEADVLLADDVLDAPSAPATDTETQHKTARGITTAQD
                     IIQRNLERPRKPKIIESSSQIVTPSAPAIIRSTATELPPIDNPAEQTRLNPSYTFETL
                     VQGKGNRMAYAGAQTIAENPGSSMYNPYFIYGSTGLGKTHLIQAIGNRIYQQHPRAKI
                     RYIHAETYVRDIMKAFRSSSFDEFKRH"
     gene            1077..1211
                     /gene="rpmH"
                     /locus_tag="PK856_10650"
     CDS             1077..1211
                     /gene="rpmH"
                     /locus_tag="PK856_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRL02866.1"
                     /translation="MKRTFQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            1215..1553
                     /gene="rnpA"
                     /locus_tag="PK856_10655"
     CDS             1215..1553
                     /gene="rnpA"
                     /locus_tag="PK856_10655"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRL02867.1"
                     /translation="MNQGFSKTERLLKTDEFSSVFALRRSRHGRYVQVLLGEVRTQPR
                     LGLVVAKKVAKRAHERNYMKRVLREWFRLHKQQLPAQDIVVRAKMKFEQEDFSAVWQE
                     MQRLLNQKAS"
     gene            1550..1759
                     /gene="yidD"
                     /locus_tag="PK856_10660"
     CDS             1550..1759
                     /gene="yidD"
                     /locus_tag="PK856_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019390225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRL02868.1"
                     /translation="MKYLLLKFVRFYQVAISPMLPPRCRYTPTCSQYAVEALQKHGAC
                     KGSMLTLRRVCRCHPFGGNGYDPVP"
     gene            1784..>2142
                     /gene="yidC"
                     /locus_tag="PK856_10665"
     CDS             1784..>2142
                     /gene="yidC"
                     /locus_tag="PK856_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRL02869.1"
                     /translation="MEFKRLMAFAVIALAILFGWNYAFPPPKKPVAQTQADAAKNANP
                     EAAQQSTLSVTDPIKVKTDVFDIEIDQATGDIRRMVLNKHDATSDASKTFVLLDDEKA
                     YTYVAQSSLLNKQGAYL"
BASE COUNT          551 a          474 c          543 g          574 t
ORIGIN      
        1 atggcgtttg aattcgtcaa acgagctgga gcgaaacgcc ttcatgatgt cacgcacata
       61 ggtttcagca tgaatatagc gaattttcgc ccgcgggtgc tgctgataaa tgcggttacc
      121 aatcgcttgt atcaagtggg ttttacccaa gcctgtgctc ccgtaaataa aatacgggtt
      181 gtacatgctc gaacctgggt tttcagcaat ggtttgtgcg cccgcataag ccatgcggtt
      241 gcctttacct tgcaccaagg tttcaaacgt gtagcttgga ttcaagcgcg tttgctcggc
      301 agggttgtct atcggtggca attcggtagc cgtggatctg atgatggcag gggcgctcgg
      361 agtaacgatt tgcgaactgc tttcaatgat tttcggtttg cgcgggcgct ccaaattgcg
      421 ttgaataatg tcttgtgctg tggtgatgcc acgcgctgtt ttgtgttggg tttcggtgtc
      481 tgtagccggt gctgaaggcg cgtctaatac atcgtcagcc aataaaacgt cggcttcatc
      541 ttcactctct acccatgcgc tgggcgtagt cgctgcaggg gctgaagcca cgctgacttg
      601 ttcacccatg ccaattttca attgtaatac aggtgcgttt ggggccaaat ttgcaacagc
      661 tgtttcgatt ttttgccaaa aatgactttt tacaaaacca aagacaaaat tgtttttggc
      721 atacaccacc caagcatcgt tttcggtgct cacggtcaaa ggtgcaatcc acatttggta
      781 ttgctgattg gataaggtgt cacgcaatgc atagagcgca tgaggccata cggcactgaa
      841 ttggcttaaa tcggcttgtg gcataaatgg gttggactta atcgacgatg tggcaggacg
      901 ctgctgcaca agaatgctaa tgcaacaaca ataagggtct attatagccg atggcagact
      961 atttatccac aggcgcttgt gggtaaatgc ggcacaaagg cgttgacaaa atggcgaaaa
     1021 cccgttgtaa tagcgcgttt atttatcgcc agatactatt aattaaggat gcaacaatga
     1081 aacgcacatt ccaaccgtca gttaccaaac gcaaacgcac tcacggcttt ttagttcgtt
     1141 caaaaacccg tggcggtcgc gccgttttgg cagctcgtcg tgccaaaggc cgtaaacgct
     1201 tggctgtata agctttgaat caaggcttta gcaagactga acgactgctg aaaacggatg
     1261 aattttcatc cgtttttgca ttgcgccgct cacgccatgg tcggtatgtg caggttttgc
     1321 taggagaagt acgcactcaa ccgcgtttgg gtttggtggt ggctaagaaa gtggccaaac
     1381 gggcacacga acgcaattac atgaaaaggg tgttgcggga gtggtttcgt ttgcacaaac
     1441 agcaactgcc agcacaagac attgtggtgc gtgccaaaat gaagtttgaa caggaagatt
     1501 tttcggcggt gtggcaagaa atgcaacgct tgttaaatca aaaggcgtca tgaaatatct
     1561 gttattgaaa ttcgtgcgtt tttatcaggt ggccatcagt ccgatgttgc cacctcgttg
     1621 tcgttatacg ccaacgtgtt cacaatatgc agttgaagcc ttacaaaaac atggagcttg
     1681 caaaggctca atgttgacat tgcgtcgggt ttgccgatgt cacccatttg gtggcaatgg
     1741 gtatgatcct gttccttaac cgatgactta aagactgaaa tcgatggaat tcaaaagact
     1801 aatggccttt gctgtgattg cattggcgat tttgtttggt tggaattacg ctttcccacc
     1861 acccaaaaag ccagttgctc aaacacaagc cgatgcggcc aaaaacgcca atcctgaggc
     1921 ggctcaacaa agcacactca gtgtgaccga tcctattaag gtcaaaaccg atgtgtttga
     1981 catcgaaatc gatcaagcca caggcgacat ccgtcgcatg gtattgaaca agcacgatgc
     2041 caccagtgat gccagtaaaa cctttgtgtt gttagacgat gagaaagcct acacttatgt
     2101 ggcgcaatct tcattgttaa acaagcaagg cgcgtatttg tt
//
LOCUS       DAOMVK010000392         2115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_15418_length_2115_cov_2.770388, whole genome shotgun sequence.
ACCESSION   DAOMVK010000392 DAOMVK010000000
VERSION     DAOMVK010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15418_length_2115_cov_2.770388"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..1035
                     /locus_tag="PK856_10670"
     CDS             121..1035
                     /locus_tag="PK856_10670"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRL02870.1"
                     /translation="MNSSVYTPMYTADRCIRHFNGLDHEVLHWPRLSRPKLLLLHGWM
                     DSADTFQATVDALPAEYDIYALSWRGCGRSAWQASGYYERMEMLQDLHQMLEHISPDE
                     SIHVCGHSMGSMLATHYACLFPERIKSLTMAEGFGMPVLEHASELHARARRFIQTSEP
                     NPVYALKSVADVAEKLQRRNPHMNMAYAHYMAHALTHETALGLAYRADPKHHLPQAHP
                     YDWHRTAPLWAHLNMPTLWIQGENLPHNHYLQRVHTLLPERQAMLPNLHDIVTLQNVG
                     HMLQWEAPQAFAAALDKFLRQVEAKSVQ"
     gene            1065..1856
                     /locus_tag="PK856_10675"
     CDS             1065..1856
                     /locus_tag="PK856_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRL02871.1"
                     /translation="MTPILTFDIETIPDAHAIRTLHDFPNDISDADVVEYALQKRRAQ
                     NGSDFLPLHLHRVVAISCCLRWSDDKVHVSTIGKIEDEEATVLRKFYELIERYTPQLV
                     SWNGGGFDLPVLHYRSLIHGISAARYWDMGDGDFGDSRDFKWNNYLSRYHMRHCDLMD
                     VLAMYNGRANAPLDQMAKLCGFPGKMGMDGSKVWDAFQQGQLQEIRDYCETDAANTYL
                     MFMRFQLLRGVYNPQEYQAEIDRLYHHLNAQPQEHWQQFCQMWQR"
     gene            1872..>2115
                     /locus_tag="PK856_10680"
     CDS             1872..>2115
                     /locus_tag="PK856_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HRL02872.1"
                     /translation="MSQLFKQVVLIGVGLIGGSLILDLKRQQLIGSVHGIDLDADNLQ
                     RALERKVVDTASTIIDDAVPQADLVILATPVGTIAGI"
BASE COUNT          524 a          579 c          520 g          492 t
ORIGIN      
        1 aggtgttcgc ttcgtcaatg tcaatcatga tgacggtggt attgtcatca cccactcaga
       61 tgcattcaat gtggatgctt tcaaaagcgc tgttcaagca ttgggatttg gtttttagac
      121 atgaattcat cggtatacac acccatgtat accgctgata gatgcatccg acattttaat
      181 ggtttagatc acgaagtgtt gcattggccg cgtttatcgc ggccaaaatt attgttactg
      241 catggctgga tggacagtgc cgacaccttc caagccaccg tagatgcact gccagcggaa
      301 tacgacattt acgccctcag ttggcgcggc tgtggccgct cagcttggca agccagcggt
      361 tattacgaac gcatggaaat gttgcaagat ttgcaccaaa tgctggaaca catcagccca
      421 gatgaatcta tccatgtgtg cggtcacagc atgggcagca tgttggccac ccactatgcc
      481 tgcctgtttc ctgagcgcat caaaagttta accatggccg agggctttgg catgcctgtg
      541 ttagagcatg catctgaatt gcacgcgcgg gcaagacgct tcatccaaac cagcgagccc
      601 aaccctgtgt acgccctcaa aagcgtggcc gatgtggcgg aaaaattaca acggcgcaat
      661 ccccatatga acatggctta cgcacattac atggcacacg ccttaaccca cgaaaccgca
      721 ttgggcttag cctaccgcgc cgaccccaaa caccacctgc cgcaagccca tccttacgat
      781 tggcaccgca ctgccccttt gtgggcgcat ttgaacatgc cgactttgtg gatacagggc
      841 gagaacctgc cccacaatca ctatttgcaa cgcgtgcaca ccttgctgcc cgagcgccaa
      901 gcgatgttac ccaatttaca cgacatcgtt acgctgcaaa acgtgggaca catgctgcaa
      961 tgggaagcac cgcaagcttt tgcggcggct ttggacaaat tcttgcgcca agttgaggct
     1021 aaatcggtac aataagcggc ctgtaagccg ccaagtgata agccatgaca ccgatactca
     1081 cctttgacat tgaaaccata cccgatgcac atgccatacg cacattgcat gatttcccca
     1141 atgacatctc cgatgccgat gtcgtggaat atgctttgca aaaacgccgc gcccaaaatg
     1201 gcagcgactt tttgccgctg catttgcacc gcgtggtggc tatttcatgc tgtttgcgct
     1261 ggtcggacga caaagtacac gtcagcacca taggcaaaat cgaagacgaa gaagccacgg
     1321 tattgcgcaa attttatgaa ttgattgaac gctatacacc ccaactggtg agctggaatg
     1381 gcggcggctt cgatctgcct gtattgcatt accgtagcct gattcatggc atcagcgccg
     1441 cgcgctattg ggacatgggc gacggcgatt tcggcgacag ccgcgatttc aaatggaaca
     1501 actacctcag ccgctaccac atgcgccatt gcgatttgat ggacgtgctc gccatgtaca
     1561 atggccgcgc caacgcccca ctcgaccaaa tggccaagct gtgtggtttc cctggcaaaa
     1621 tgggcatgga tggttccaag gtttgggatg ccttccaaca aggtcaattg caagaaatcc
     1681 gcgactattg tgaaaccgat gcggccaata cctatttgat gttcatgcgc tttcaattgt
     1741 tgcgcggcgt ttacaaccca caagaatacc aagccgaaat cgaccgttta taccaccacc
     1801 tcaatgccca gccacaagag cattggcagc aattttgcca aatgtggcaa cgctaaaggc
     1861 ggcctgtaac catgtctcaa ttgttcaaac aagtggtgct catcggcgtg ggcttgatag
     1921 gcggctcatt gattttggat ttgaagcgcc aacaactcat aggctcggtg cacggcattg
     1981 atttggatgc cgacaatttg cagcgcgcct tagagcgcaa agtggtggat accgcctcca
     2041 ccatcataga tgacgctgtt ccccaagccg atttggtgat tttggccacg cccgttggca
     2101 cgattgcggg catta
//
LOCUS       DAOMVK010000393         2111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_15466_length_2111_cov_2.722763, whole genome shotgun sequence.
ACCESSION   DAOMVK010000393 DAOMVK010000000
VERSION     DAOMVK010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15466_length_2111_cov_2.722763"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>291)
                     /locus_tag="PK856_10685"
     CDS             complement(<1..>291)
                     /locus_tag="PK856_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HRL02873.1"
                     /translation="LKQKVVYFLALCVVLFSNLAHAAIDPNSILPVEKAYQPQLFATE
                     DGVSLQFTIADGYYLYKDKLEFSSDGPSLGKPEFSPAQSTTDEFFGTQAVYFK"
     assembly_gap    292..310
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(426..1694)
                     /locus_tag="PK856_10690"
     CDS             complement(426..1694)
                     /locus_tag="PK856_10690"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRL02874.1"
                     /translation="MNQALSSLISHKLQHLSLPLCIQLDTGDSFGPNNAPVRLHIHRL
                     STLKHLAQGHIGKLASDIVEGRADLHGNMRDVMAVATELLAKSPVQAHNSWWARFFKY
                     SHGLAQHSLPQDAAHIRFHYDVSDDFYALWLDPRRVYSCAYYRDEHMDLAAAQTAKLD
                     LICRKLYLQADEQVLDIGCGWGGFLIYAAEHYGILGTGITLSHNQYAYAQALIAQKGL
                     QERITLHLCDYRQFKAPHLFDKIASIGMFEHVGRANMTAYFQTLRRFIKPGGLILNHG
                     ITAGGTRNKQLGSGMGDFIEKYIFPGGELLHISDVLHHVADANLEAVDIENLRPHYAQ
                     TLWAWSDSLEAALPAAQSALEQNLSAEQALQVLKAYRLYLAGCAMSFEQAWISLHQLL
                     VCSPDGDVNHGRLRGAQSDYPYTRDGMYLS"
     gene            complement(1803..>2111)
                     /locus_tag="PK856_10695"
     CDS             complement(1803..>2111)
                     /locus_tag="PK856_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRL02875.1"
                     /translation="SKIKIKSINFMRGRTFQHKFLIIDEAQNLTSKQMKTMITRAGEG
                     TKIVCMGNLAQIDTPYLSEGSSGLTYLVDRFKNWPHFGHITMQHGERSRLAAHAVAVL
                     "
BASE COUNT          530 a          485 c          548 g          529 t
ORIGIN      
        1 tttgaaatac acggcttggg tgccgaaaaa ttcatcggtg gtggattggg caggactgaa
       61 ttcaggcttg cccaagcttg ggccatcgct gctaaattct aatttgtcct tgtaaaggta
      121 atagccatcg gcaatggtga attgcaaaga aacgccgtct tcggtggcaa acagttgcgg
      181 ttggtaggct ttttcaacag gtaaaatgga attggggtcg atggcggcgt gtgccaaatt
      241 gctgaataag accacacaca aggccaaaaa atacaccact ttttgtttta annnnnnnnn
      301 nnnnnnnnnn gcccatacct attgcaccat aaccttaatg atgatttttc aaggctttca
      361 ttctaacaca gccatttcaa gccatgcctt gaaagcccgt atgcggcctg taaacccatg
      421 taaacctatg acaagtacat tccatctcgg gtgtaagggt agtcactttg ggcaccacgc
      481 aggcggccat ggttcacatc gccatcgggt gagcatacca acagttggtg caagctgatc
      541 caagcctgtt caaaactcat cgcacacccc gctaaataca aacgataggc tttgagcact
      601 tgcaaagctt gctcggcaga taggttttgt tccagtgccg attgtgctgc gggcagggca
      661 gcttcgagcg aatcagacca cgcccacaga gtttgggcat agtgtgggcg caggttttcg
      721 atgtccacgg cttctaagtt ggcatcggcc acatggtgta acacatcgct gatatgcaat
      781 aactctccac caggaaagat gtatttttca atgaaatcgc ccataccgct gcccaattgc
      841 ttgttgcgcg tgccgcctgc ggtgatgccg tgattcaaaa tcaggccacc tggtttgata
      901 aaacggcgca aggtttgaaa atatgccgtc atgttggcgc gccccacgtg ctcaaacatg
      961 ccaatagaag caatcttgtc aaaaagatgc ggggctttga attggcggta gtcacacagg
     1021 tgtaaggtga tgcgctcttg caagcctttt tgtgcaatta aggcttgggc ataggcatat
     1081 tggttgtgtg acaaggtgat gcccgtgccc aaaataccat aatgctcggc tgcataaatc
     1141 aaaaatccgc cccaaccgca gccaatgtcc aagacctgct catcggcctg taaatacagt
     1201 tttcgacaaa tcaaatccaa tttggcggtt tgggctgcgg ccaaatccat gtgctcatca
     1261 cggtagtaag cgcaggaata tacgcggcgt gggtctaacc acaaggcgta aaaatcatcg
     1321 gacacatcgt aatgaaaccg tatgtgtgcg gcatcttgag gcaaagaatg ctgggcgaga
     1381 ccatggctgt atttgaaaaa gcgtgcccac caagaattgt gggcttgtac aggtgatttg
     1441 gccaacagct cggtagccac ggccatcaca tcgcgcatat tgccatgcaa gtcggcacgg
     1501 ccttcaacga tgtcgctggc aagtttgccg atgtggcctt gcgccaaatg cttgagggtg
     1561 cttaaacggt gaatgtggag cctgactggg gcattattag ggccaaaaga atcacctgta
     1621 tccagttgta tgcacaatgg cagggacagg tgttgcagtt tgtggctaat caaagaagag
     1681 agggcttgat tcattgtgca cagcctttct tgagtgcaga agacttgggc cagtgaaaag
     1741 aggtgcggcc tgagtttaca ggccgcttgt gtcacaaaga gggtgctgcc tctactgact
     1801 gcttacaata cggccaccgc atgggctgcc aaacgtgagc gttcgccgtg ttgcatggtg
     1861 atgtgaccaa agtgtggcca gtttttaaat ctatccacca aataagtcaa accactcgag
     1921 ccttcgctga ggtagggcgt gtcaatttgg gctaagttcc ccatgcacac gattttggtg
     1981 ccttcgcccg cgcgggtaat catggttttc atttgtttag aagtgaggtt ttgcgcctcg
     2041 tctatgatga gaaatttgtg ttggaaggtg cgaccacgca taaagttaat cgatttgatt
     2101 ttgattttgc t
//
LOCUS       DAOMVK010000394         2111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_15467_length_2111_cov_2.484436, whole genome shotgun sequence.
ACCESSION   DAOMVK010000394 DAOMVK010000000
VERSION     DAOMVK010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15467_length_2111_cov_2.484436"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="PK856_10700"
     CDS             complement(<1..957)
                     /locus_tag="PK856_10700"
                     /inference="COORDINATES: protein motif:HMM:NF020076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HRL02876.1"
                     /translation="MKPSPILLSVLMAWAVFAVDARVIVSQPSMVSMNVPKQAQTQAI
                     VLQKLAVDVEVLPALAQTQLTMAFYNPNHRPLEGELVVPLAEGQKITAMALDINGEMR
                     AAVPVPKAKGQQVFEAVERRQVDPALLEQISGNQFRLRVYPIPAQGTRTVSITVNQAL
                     SHQDGHLVYQLPVRFGKVGNAHMRINSSLNSAESFQLPTGFQSVAQANGGFVAQAKGM
                     SQGLERVLTWQFAAPKTTQVLWQNVGNERYFLANIPVKSDTYARRPPTHLGIVWDASL
                     SRRDADFATQLAVLAAYFKAMPDAEVSVQIVRGTGSDDVRTYR"
     gene            complement(1112..1606)
                     /locus_tag="PK856_10705"
     CDS             complement(1112..1606)
                     /locus_tag="PK856_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02877.1"
                     /translation="MPFSLLDHADLWFDGSNLAFYVPHTTASPGEDMIKPQTPPPSEQ
                     SNTSPLFTDKQEMLFDFFDKFGGLSWSEKLQAASYSGFPYKMALLGSWGTLFFGWLYL
                     AYIGLLHPAIVLLLVLMCSITLCWYFDAMVFVFPYVLAQIWAAIRTPYNRYLLQQHAW
                     HAWW"
     gene            complement(1692..>2111)
                     /locus_tag="PK856_10710"
     CDS             complement(1692..>2111)
                     /locus_tag="PK856_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HRL02878.1"
                     /translation="QADHPECASPQLSWSGAPAGTKGFAIAMHDPDAPTGGAGWWHWM
                     LLVLPADVTTLARGAGVADGSGLPAGVRQMRHDGGGVGYMGCMPPVGERHRYIHTVYA
                     LDTDQIDLPADATTSMAGFMINAHCLAKASTQHLFER"
BASE COUNT          560 a          568 c          515 g          468 t
ORIGIN      
        1 gcgatacgtg cgcacatcat cgctgcctgt accgcgcaca atttgcacac tcacttcagc
       61 gtcgggcatg gctttgaaat acgctgccaa caccgccaac tgcgtcgcaa aatcagcatc
      121 gcgtctggac aaagaggcat cccaaacgat gcccaaatgc gtgggtgggc ggcgcgcgta
      181 agtatcactt ttgacaggaa tattggccaa gaaataacgc tcattgccaa cattttgcca
      241 caacacctgt gtggttttgg gcgctgcaaa ctgccaagtc aatacccgct ccaaaccttg
      301 gctcatgcct ttggcctgtg ccacaaagcc gccattcgct tgggccacgc tttgaaaacc
      361 tgtgggtagt tgaaacgatt ccgcgctgtt taagctgctg ttgatacgca tgtgagcatt
      421 gcccactttg ccaaaccgaa caggcaattg gtataccaag tgcccatctt gatggctcaa
      481 tgcttggttc acggtgatgg aaaccgtgcg tgtgccttgg gcaggaatgg gatacacgcg
      541 cagacgaaat tgattgccac tgatttgctc tagcaacgct gggtcgactt gtcggcgctc
      601 tactgcttca aacacctgct gacctttggc tttgggcaca ggcacagccg cgcgcatttc
      661 gccattaatg tccaaagcca tggccgtgat tttctggccc tcggccaagg gcacgaccaa
      721 ttcaccttct aatggtcggt gattgggatt gtaaaacgcc atcgtcaatt gggtttgcgc
      781 taatgcgggc aaaacttcca catccacagc cagcttttgc aagactatgg cttgcgtctg
      841 cgcttgcttg ggaacattca tgctgaccat ggaaggctgg ctgacaatta cgcgggcatc
      901 aaccgcaaat acagcccatg ccatgagcac actgaggaga atcggagaag gtttcatgtt
      961 gagccttgtg gagggtttac aggccgctta tgtcatggcc accacagcca acaagacatc
     1021 acgccatcat catgaaacga atatgcggtt tttattcaaa aaaagcaatg tcagaaatca
     1081 ctggttgcag aacgcaagca agcttttgct gttaccacca cgcatgccaa gcatgttgtt
     1141 gcagcaaata gcggttataa ggggtgcgta tcgccgccca aatttgcgcc aacacataag
     1201 gaaacacaaa gaccatggca tcgaaatacc aacacaaggt aatgctgcac atcaatacca
     1261 gcagcaacac tatcgcggga tgaagcaaac caatgtaagc caaatacaac cagccaaaga
     1321 ataaggttcc ccagctgccc aacaacgcca tcttgtaagg aaaacctgag taagaagcgg
     1381 cctgtaactt ctcagaccaa cttaagccgc caaatttatc aaagaaatca aacaacatct
     1441 cttgtttgtc tgtgaacaaa ggagaagtgt tggattgctc agacggtggt ggtgtttgcg
     1501 gcttaatcat atcctcccca gggctcgcag tggtgtgtgg cacataaaat gccaaatttg
     1561 agccatcaaa ccacaaatcc gcatggtcta gcaaactaaa tggcatatat ttaccgttct
     1621 cacggtgttt gtaacagatg ggcaataaaa aagcctcatt aaagaggcta agatacatgc
     1681 ttgtctgaaa ctcagcgttc aaacaaatgc tgggtgctgg ccttggcaag acaatgtgcg
     1741 ttaatcatga aacctgccat agaagtggtc gcgtcggctg gtaagtcaat ttggtctgtg
     1801 tccaatgcat acactgtatg gatgtagcga tgacgctcac ccacaggcgg catgcagccc
     1861 atatagccca cgccgccacc atcgtgacgc atttggcgca cacccgcagg caagccactg
     1921 ccatcggcca cgcccgcacc gcgcgccaat gttgtcacat cggctggcaa aaccaacagc
     1981 atccaatgcc accaacctgc cccgcctgtg ggtgcatcag ggtcgtgcat ggcgatggca
     2041 aaacctttcg tgccagcagg cgcgccgctc cagcttaatt gtggcgaggc acactcagga
     2101 tggtcggctt g
//
LOCUS       DAOMVK010000395         2106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_15523_length_2106_cov_4.554851, whole genome shotgun sequence.
ACCESSION   DAOMVK010000395 DAOMVK010000000
VERSION     DAOMVK010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15523_length_2106_cov_4.554851"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..710)
                     /locus_tag="PK856_10715"
     CDS             complement(126..710)
                     /locus_tag="PK856_10715"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRL02879.1"
                     /translation="MLKSAIISSLLFGASALAFAAPVAYQVDPTHTATVFSWNHLGFS
                     HPSANFKDIRGVIHYDAQKPSNSSVNITIPVNSVNTNVPLLDSKIQDAEWFDVAKHPN
                     ITFKSTKVELLDGKRLKVTGNLTVKGIAKPVVLVAKLNHMGAHPMVKKPAIGFDATTH
                     FKRSDFGMTDLIPSVSDRIDIKITTEAIANGKVF"
     gene            complement(916..1773)
                     /locus_tag="PK856_10720"
     CDS             complement(916..1773)
                     /locus_tag="PK856_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRL02880.1"
                     /translation="MKRVQSIYPKPAMHWVGDGFPVHSLFSYHQQRGQNISPFLLLDH
                     AGPTQFPPSQHLRGVGQHPHRGFETVTIVYKGEVSHHDSTGQGGTIGEGEVQWMTAGR
                     GIIHEEYHSEHFAQHGGDFEMVQLWVNLPAHQKMTAPNYQAISRNDIPTVTLEHQAGV
                     LRVIAGTFAEAIGPAHTHTPMNVWDVHLNAHSELSVDLPEQWNTFALVLDGTVLFNQD
                     TVARAGQLVQFTAAGERLDIASQTNATILILSGEPIREPVVGHGPFVMNTAAEITQAF
                     EDFSKGEFV"
     gene            1865..>2106
                     /locus_tag="PK856_10725"
     CDS             1865..>2106
                     /locus_tag="PK856_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRL02881.1"
                     /translation="MKQINDLNQMYYFAQVVHYQGFSAASRELGIPKSRLSRQVAQLE
                     EHLQVRLIQRNTRHFYLTEIGHRYYQHCQAMLIEAEA"
BASE COUNT          513 a          505 c          517 g          571 t
ORIGIN      
        1 tcttggatga attggttggc gtattgtgcg caatctgctg agtgcgacac attgtgttcg
       61 gtggtattgc tcatgttcgt ctcctgagta aggattgcgc gcaagcacaa gtttgattgc
      121 tgtctttaaa agaccttgcc attggcaatg gcttcagtgg tgattttgat gtctatgcga
      181 tcgctgacac tcgggatcaa atcggtcatg ccaaaatcag aacgcttaaa atgggtggtg
      241 gcatcaaaac cgatggcggg ctttttcacc atgggatgcg cgcccatgtg attcaatttg
      301 gccaccaaca ccaccggttt ggcgatgcct ttgacggtta aattgcctgt taccttcagg
      361 cgtttgccat ccaagagctc aaccttggtg cttttgaacg tgatatttgg gtgtttggcc
      421 acatcaaacc attcggcatc ttggattttg ctgtccaata aaggcacgtt ggtgttcacg
      481 ctgttcacag gaatggtgat gttgaccgaa gaattgctcg gcttttgggc gtcataatgg
      541 atgacccctc gaatgtcttt gaaattggca cttgggtgtg aaaaacccaa atggttccaa
      601 ctgaacacag tggccgtgtg ggtgggatcg acttggtagg ccacaggtgc ggcaaacgcc
      661 agcgcgctgg ctccaaacaa caaactgctg atgatggcag atttcaacat aaaataagcg
      721 tcctgtaaca atgtgtgttg tctcagcaat cacacaatat gggtttaact ctaaaatagt
      781 atctgtaaac aatggataac agcatggttt gcttggctca ccaggatggc gcgctactca
      841 tgattcaatt gactgcctga ctgcttttga actgatttaa ggagcgagtc caaaccatcg
      901 gcaatcactt gtgcatcaaa caaactcacc cttactaaaa tcctcaaaag cttgggtgat
      961 ttctgcggcg gtattcatga caaatggccc atgccccacc actggctccc gtatcggttc
     1021 tccactgaga attaaaatcg ttgcattggt ttggctcgcg atgtctaagc gttcacctgc
     1081 cgccgtaaac tgtaccaatt gccctgctcg agccactgtg tcttggttaa acaatactgt
     1141 cccgtctaaa accaaggcaa aagtgttcca ttgttcgggc aaatccacac tgagttccga
     1201 atgggcattc aaatgcacat cccacacatt cattggtgtg tgcgtgtgtg ctggaccgat
     1261 tgcttctgca aacgtgcctg caatcaccct tagcacgcct gcttgatgct ccaaggtcac
     1321 agtgggtatg tcattgcggc tgatggcctg atagtttgga gcggtcattt tttgatgggc
     1381 aggcaagttc acccacagtt gcaccatttc aaaatcccca ccgtgttgag cgaagtgctc
     1441 gctgtggtat tcctcatgaa tgatacccct gccagcggtc atccactgta cctcgccctc
     1501 gcctatggtg ccaccttgtc ccgttgagtc atggtgcgac acctcacctt tgtagacgat
     1561 ggtcactgtt tcaaaaccgc ggtgggggtg ttgccccacg ccacgcaaat gttggctggg
     1621 tggaaactga gttggccctg cgtggtctag caagagaaat gggctgatgt tttggcccct
     1681 ttgttggtgg tagctgaaca aggagtgcac tggaaatcca tcgcctaccc aatgcatggc
     1741 tggttttggg taaatgcttt gaacacgctt catgggcaag actccttggt ttcagatagg
     1801 gtctatatta aaatatagaa cattatggca gtagtcagtt aaatcagaat ggattgttcc
     1861 atcaatgaaa caaataaacg atttaaacca aatgtattat tttgctcaag tggtacatta
     1921 ccaagggttc tctgcggcca gccgcgagct gggcattccc aaatcacgcc tgagtcgcca
     1981 agtggcacag ctagaagagc atctgcaggt aaggctgata caacgcaata cccgtcattt
     2041 ttacttaacc gaaattggcc accgctatta tcaacactgc caagccatgc tcattgaagc
     2101 cgaagc
//
LOCUS       DAOMVK010000396         2104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_15549_length_2104_cov_2.882870, whole genome shotgun sequence.
ACCESSION   DAOMVK010000396 DAOMVK010000000
VERSION     DAOMVK010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15549_length_2104_cov_2.882870"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="PK856_10730"
     CDS             complement(<1..786)
                     /locus_tag="PK856_10730"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRL02882.1"
                     /translation="MFKQSLIGLALLSCSISALALTPRLIPEENIGKADNLYGLSAKE
                     QAPTYRNMDFLYPVRVVNVGKKPLPLSASTDELAVTYTVDGKSINTDTFMRRNQVAGL
                     LVMRDGKILVERYAQGNTPDTRWTSFSIAKSVSSTLIGAAIEDSKIGSLQDDVTRYVP
                     ALKGSAYEGVTVGQVLDMSSGVKWNETYRDPKSDRRQMFNAQLSNKPGSIVQVMAGLK
                     RAHPAGNAFNYSTGESHLQAEILKGATGKTSSAYLSEKIWGPLG"
     gene            complement(940..>2104)
                     /locus_tag="PK856_10735"
     CDS             complement(940..>2104)
                     /locus_tag="PK856_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009115341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3520 domain-containing protein"
                     /protein_id="HRL02883.1"
                     /translation="WLQDAKLLRIVLEAQEQKTAEMPPAHLVFLVDVSGSMTAENKLP
                     LVQKTLRLLTEQLRAEDMVSIVTYASGTDVVLQAATGREKKAIMAAIDKLKAGGSTAG
                     ANAMEKAYQIAKNHQLKDGINRILMATDGDFNVGTVDFSTLKGRVAEQRKNGISLTTL
                     GFGTGNYNEQLMEQLADAGDGNYSYIDTEKEAKKVLQQQLSSTLSTVASDVKIQVEFN
                     PNTVSQYRLIGYENRVLNQEDFNNDNKDAGEIGAGHVVTALYELVPQGKQGWLSPSRY
                     QNQAVKSGLGHEYAVVNVRYKPVGKSSSVLLSRPVNTDSKPLAQASSDTRFAVAVASY
                     GQLLRGGDMVGKQTWQDVQQLAQGAKGKDAFGLRAEFLELLGKAQQLSSQAKQ"
BASE COUNT          525 a          572 c          437 g          570 t
ORIGIN      
        1 gcccaatggc ccccaaatct tttcagacaa atacgcagaa gacgttttgc cagtagcacc
       61 ttttaaaatc tcggcctgta aatgcgattc acccgtactg taattgaagg cattgcctgc
      121 tggatgggca cgcttcaaac cagccatgac ttgtacaata ctgcctggct tgttagacaa
      181 ttgcgcattg aacatttggc ggcggtctga tttgggatcg cggtaagtct cgttccattt
      241 gacacctgaa ctcatgtcca acacttgccc aaccgtcacg ccctcatatg cactgccttt
      301 gagtgcaggc acataacggg tgacatcatc ttgcaagctg ccaattttgc tgtcttcaat
      361 cgccgcgccg attaaagtgg aagaaacaga cttggcaatg gagaatgatg tccaacgcgt
      421 gtcgggtgtg ttgccttgcg catagcgttc caccaaaatc ttgccatcac gcatcaccaa
      481 taaacccgcc acttgattgc ggcgcatgaa cgtgtcggta ttgatgcttt ttccatcaac
      541 agtgtatgtg accgctaatt catccgtact ggcactcaag ggcaaaggtt tcttacccac
      601 attcaccacc cgaacgggat acaaaaaatc catgttgcga taagttggcg cctgctcttt
      661 ggcagacagc ccatacaaat tgtcggcctt gccaatattt tcttcaggga tgagacgcgg
      721 ggtcagcgcc aaagcgctga ttgaacaaga caataaagcc aaacctatga gtgattgctt
      781 aaacatagaa acccctagca tgtgtattaa tgaaatgaaa caagaccatg accacaatgt
      841 atccagcata aacaaaacgc attaagctgt cgatgtggtc atcgctcttg tctcaaatct
      901 agaaaagctt gtacagcttg gttcacacaa gacacacgat tattgcttgg cttggctact
      961 taattgctgc gccttgccca gtaattctaa gaattccgca cgcaaaccaa acgcatcttt
     1021 gcccttagcc ccttgtgcca attgctgcac atcttgccaa gtttgtttgc ccaccatgtc
     1081 tccaccgcgc aacaattgcc cataacttgc cacagccact gcaaaacgcg tgtcgctgct
     1141 ggcttgagcc aaaggcttgc tgtcggtgtt tacaggccga ctcaacaaca cactgctgga
     1201 tttccccact ggtttgtaac gcacattcac caccgcatat tcatggccca aacccgattt
     1261 cacagcctga ttttgataac gagagggact gagccaacct tgtttgccct gcggcaccaa
     1321 ttcatacaaa gcagtcacca catggcctgc gcctatctcg cccgcatctt tattgtcatt
     1381 attgaagtct tcttggttca gaacacggtt ttcataacct atcaaacggt attgcgacac
     1441 ggtgttggga ttgaactcga cttggatttt gacatcagag gccacagttg aaagcgtgga
     1501 cgacaattgc tgttgcaaca ctttcttggc ttctttttcg gtatcgatgt agctgtaatt
     1561 accatcgcct gcatccgcca attgctccat caattgctca ttgtaattgc ctgttccaaa
     1621 gcccaaagtg gtcaaggaaa tgccgttttt ccgctgctca gccacgcgcc cttttaatgt
     1681 ggaaaaatcc accgtaccca cattaaaatc gccgtctgtg gccattaaaa tgcggttgat
     1741 gccatctttc aattgatggt ttttggcgat ttgataggct ttttccatcg cattcgcgcc
     1801 cgcagtgctg ccacctgcct tcaatttgtc tattgcagcc ataatggctt ttttctctcg
     1861 ccccgtagca gcctgtaaca ccacatctgt tcctgaggca taagtcacaa tgctgaccat
     1921 gtcttcagcg cgcaattgtt ctgtcaacaa gcgtaaagtt ttttgcacca atggcagttt
     1981 gttctctgcc gtcatagagc cagacacatc caccaaaaac accagatgcg caggcggcat
     2041 ttctgcggtt ttttgttctt gcgcttccaa cacaatgcgc agcaatttcg catcttgcaa
     2101 ccat
//
LOCUS       DAOMVK010000397         2101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_15591_length_2101_cov_3.288856, whole genome shotgun sequence.
ACCESSION   DAOMVK010000397 DAOMVK010000000
VERSION     DAOMVK010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15591_length_2101_cov_3.288856"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2101
                     /locus_tag="PK856_10740"
     CDS             <1..>2101
                     /locus_tag="PK856_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRL02884.1"
                     /translation="GIAVGMATNIPPHNLREVINACIELLHNPDLSIDEIIDLIPAPD
                     FPTGATIYGLSGVREGYRTGRGRVVMRGKTHIEPLPKNPDREAIIIDEIPYQVNKAKL
                     VEKIGELVREKILEGVSDLRDESDKSGMRVVIELKRNENAEVVLNQLYKMTQLQDSFG
                     INMVALVDGQPRLLNVKQILSEFLRHRREVVTRRTMFELKKARERGHILEGLAVALSN
                     VDEIIALIKASATPPEAKQALLARAWQSDLVNDMLARVEGDMSLVRPENVDEACGLHN
                     GAYHFSEAQAQAILEMRLQRLTGLEQDKIIAEYKDIMATILDLLDILSKPERINQIID
                     DELVAIKEQYGDERRSEINPFGGDIADEDLIPPREMVVTLTHGGYIKTQHVDDYQAQK
                     RGGRGKQAAATKDEDFIESLFVANTHDYLMCFTNLGRCHWIKVYKLPEGGRNSRGRPI
                     SNVIQLEENEKVSAILAVREFPEDQYVFFATAQGLVKKVQLSAFKNVRSQGIKAIALK
                     EGDSLVGVAQTSGQDDIMLFSNLGKAIRFNEYWERSNTETDNEDQEDIEATSDTEGEE
                     DGSEVSSKPANGVRISNRGSGGLRGMRLPSDGRIVSLITFAPECEQGELQVLTATANG
                     YGKRTPIADYSRKGKGGQGNIAINTGERNGELVAATLVNDCDDLMLITSGGVLIRTKV
                     NQVRETGRAAQGVRLINL"
BASE COUNT          585 a          525 c          539 g          452 t
ORIGIN      
        1 ggcattgccg tgggcatggc caccaacatt ccgccgcaca atttgcgtga agtcatcaat
       61 gcctgtatcg agctgttaca caatcctgat ttgagcatcg atgaaatcat agacttgatt
      121 cccgcccccg actttcctac gggtgccacc atttatggtt tgagtggtgt gcgtgaaggc
      181 taccgcacag gtcgcggccg cgtggtgatg cgtggtaaaa cccacattga gcctttgcca
      241 aaaaatccag accgcgaagc catcatcatt gatgaaatcc cttaccaagt gaacaaagcc
      301 aaattggtcg aaaaaatcgg cgaattggtg cgtgaaaaaa tcttggaagg cgtgtctgat
      361 ttgcgcgatg agtctgacaa atcgggcatg cgtgtagtga ttgagctcaa gcgcaatgaa
      421 aatgcagaag tcgtgctcaa ccaattgtac aaaatgacgc aattgcaaga cagcttcggc
      481 atcaatatgg tggctttggt cgatggccaa ccgcgcttgc tcaacgtcaa acaaatttta
      541 agcgaattct tgcgccaccg tcgtgaagta gtgacgcgtc gcaccatgtt tgaattgaaa
      601 aaagcacgtg aacgcggcca tattttggaa ggtttggccg ttgccttgtc caatgtcgat
      661 gaaatcatcg ctttgattaa ggccagcgcc acccctccag aagccaaaca ggctttgctt
      721 gcacgtgcat ggcaatctga cttggtcaac gacatgcttg ctcgcgtaga aggcgacatg
      781 agtttggtgc gtcctgaaaa cgtagacgaa gcctgtggtt tacacaatgg cgcttaccat
      841 ttcagcgaag cgcaagcgca agcgattttg gaaatgcgct tacaacgctt aacaggcttg
      901 gaacaagaca aaatcatcgc cgaatacaaa gacatcatgg ccacgatttt ggacttactc
      961 gatattttgt ccaaaccaga acgcatcaac caaatcattg atgacgaatt ggtggcgatt
     1021 aaagagcaat acggtgacga acgccgctca gaaatcaacc cattcggtgg cgacattgcc
     1081 gatgaggatt tgattccacc gcgcgaaatg gtggtgactt taacccacgg tggctacatc
     1141 aaaacccaac acgttgatga ctaccaagca caaaaacgtg gcggtcgtgg caaacaggca
     1201 gcagccacca aagacgaaga ctttatcgaa tcattgttcg tggccaatac ccatgattac
     1261 ctgatgtgct tcaccaattt gggccgttgc cattggatta aagtgtacaa attgccagaa
     1321 ggtggtcgca acagccgcgg ccgcccaatc agcaatgtga tacagctgga agaaaacgaa
     1381 aaagtcagcg ccattttggc cgtgcgtgaa ttccctgaag accaatatgt gttctttgcc
     1441 accgcccaag gcttggtgaa aaaagtccaa ttgtcggcct ttaaaaacgt gcgcagtcaa
     1501 ggcatcaaag ccattgcctt gaaagaaggc gacagcttgg tgggtgtggc acaaaccagt
     1561 ggccaagacg acatcatgtt gttctctaac ttgggcaaag ccatacgctt taatgagtat
     1621 tgggaacgca gcaacaccga aaccgacaat gaagaccaag aagacatcga agccacaagc
     1681 gataccgaag gcgaagaaga cggcagcgaa gtgagcagca aacctgccaa tggcgtgcgc
     1741 atctccaacc gtggcagtgg cggcttgcgc ggcatgcgct tgcctagtga tggccgcatc
     1801 gtcagcctga ttaccttcgc acctgagtgt gaacaaggcg aattgcaggt attgactgcc
     1861 actgccaatg gttatggcaa acgcacgccg attgccgatt acagccgcaa aggcaaaggt
     1921 ggccaaggca acatcgccat caacactggc gagcgcaatg gcgaattggt ggccgcaact
     1981 ttggtcaacg attgcgatga cttgatgctc atcacctctg gcggcgtgtt aatccgcacc
     2041 aaagtcaacc aagtgcgcga aacaggccgt gccgcccaag gcgtgcgctt gattaacttg
     2101 g
//
LOCUS       DAOMVK010000398         2099 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_15616_length_2099_cov_2.955969, whole genome shotgun sequence.
ACCESSION   DAOMVK010000398 DAOMVK010000000
VERSION     DAOMVK010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15616_length_2099_cov_2.955969"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PK856_10745"
     CDS             complement(<1..206)
                     /locus_tag="PK856_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRL02885.1"
                     /translation="MSAAQAAVNTKVHLFSGFLGTGKTTAIKSLLAQKPEHEKWVIIV
                     NEFGEIGIDGAVLTEDNIPVAEISG"
     gene            complement(182..670)
                     /locus_tag="PK856_10750"
     CDS             complement(182..670)
                     /locus_tag="PK856_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRL02886.1"
                     /translation="MNLAQLKQQILNNCKHHKVQVTAQREQVLDLILAEHGVIKAYDV
                     LSKLQAASSTAVAPPTAYRALDFWVEQGVLHKVTAVNGYIVCRHNGEHEHDEHGDCTH
                     EPHQQAIILVCTQCHAVDEQNLTHEWQQLQQGLARTGFQLDEEHVALTGICQQCQQLK
                     QQ"
     gene            755..1204
                     /locus_tag="PK856_10755"
     CDS             755..1204
                     /locus_tag="PK856_10755"
                     /inference="COORDINATES: protein motif:HMM:NF013487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRL02887.1"
                     /translation="MKLLSVLCLLSFAISTSACSASEEVKRGPHSQARHQAFTQMMPT
                     YTKMVQMTNGNNGYQKAEFAQLVAQFETEAQAPFKHFQSDGQGLDGKAKAEVWQNSTA
                     FEQQQTAFFDSIKGLKTASEHSNLDEIRAAIKQVDKQCMSCHATYRD"
     gene            complement(1277..1993)
                     /gene="pnuC"
                     /locus_tag="PK856_10760"
     CDS             complement(1277..1993)
                     /gene="pnuC"
                     /locus_tag="PK856_10760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HRL02888.1"
                     /translation="MQLFKEWTWFERIWLMVFTAINVYLYVKWDSGLIGLISAMTGMM
                     CVVLVAKGKISNFLFGVVNTATYAYISYGYGLYGESMLNALFYLPTQFIGLWMWQRHR
                     AVNKVRDEDIEIKRLNVKGWAILVASIAVGAYVYMHVLMALNAQQVRIDSVAVVMSVA
                     AQILLTLRYAEQWVLWILVNVLSIALWVVTLNQSGGSDYSMPVMWTAFLINSIYGWIN
                     WLKLQQGKAAQMSVAQPIKL"
BASE COUNT          546 a          566 c          460 g          527 t
ORIGIN      
        1 cctgaaattt cggccacggg aatgttgtct tcggtcaaaa ccgcgccatc aatgccgatt
       61 tcgccaaatt cattgacgat gatgacccat ttttcatgct ctggtttttg tgccaataaa
      121 gatttgatgg cggtggtttt gcccgtgcct aaaaagccgg aaaacaaatg tactttggtg
      181 tttactgctg cttgagctgc tgacattgct gacatattcc tgttaatgcg acgtgttctt
      241 catccaattg aaaccctgtg cgcgccaaac cttgctgcaa ttgctgccac tcatgggtca
      301 ggttttgctc gtccacggca tggcattgcg tacacaccaa aataatcgct tgctgatgcg
      361 gttcatgagt gcaatcacca tgctcatcgt gctcatgctc accattgtgg cggcacacga
      421 tatagccatt caccgccgtc actttgtgca acacgccttg ctctacccaa aaatccaagg
      481 cacggtaagc cgtgggcggc gccacagccg tgctggaagc ggcctgtaat ttggataaaa
      541 catcgtaggc tttgatgacg ccgtgttcgg ccagaatcaa atccaaaacc tgctcacgct
      601 gcgcggtgac ttgcaccttg tggtgtttgc aattgttgag gatttgctgt tttagttgcg
      661 ctagattcaa gtctggatgg tctcgctatg atagacttgc ccatcatacc gtgtcgttat
      721 tgttgtgaca aatcacttag aaagttatgg atttatgaag ttgttgtctg tcttgtgcct
      781 actgagtttt gctatttcta cctccgcctg ctctgcttct gaagaagtca aacgtggccc
      841 gcacagccaa gcgcgccatc aagcgtttac gcagatgatg cccacttata ccaagatggt
      901 gcaaatgacc aatggcaaca atggctatca aaaagccgaa ttcgcgcaat tggtggccca
      961 atttgaaacc gaggcacaag cgccatttaa gcattttcaa tccgatgggc aaggtttgga
     1021 tggtaaggcc aaagccgaag tgtggcaaaa cagtactgcg tttgagcagc aacaaacagc
     1081 gttttttgac agcatcaaag gcttgaaaac cgcgtctgag cacagcaatt tggatgaaat
     1141 ccgcgcagcc atcaagcaag tcgacaagca atgcatgtcg tgccacgcca cttaccgcga
     1201 ttgattgaaa ttgataaaca agcggcctgt aaacgcggtg aatccgttta caggccactt
     1261 attttccata tgacccttaa agcttgatag gctgagccac gctcatctgc gccgctttgc
     1321 cttgctgcaa cttcagccaa ttgatccagc cgtatatcga attgattaaa aacgccgtcc
     1381 acatcacagg catggagtaa tcgctgccgc ccgattggtt caaggtcacc acccacaaag
     1441 cgatggacaa cacattcacc aaaatccaca acacccattg ttcggcataa cgcagcgtca
     1501 gtaaaatttg cgccgccaca ctcatcacca ccgccaccga atctatgcgc acttgctggg
     1561 cattcaatgc catcaacaca tgcatgtaaa cataagcacc caccgcaata ctcgccacca
     1621 aaatggccca accttttaca ttcaaacgtt tgatttcgat gtcctcatcg cgcactttgt
     1681 ttaccgctcg gtgtcgctgc cacatccaca agccgatgaa ttgcgtgggt aaataaaaca
     1741 aggcattgag catggattcg ccgtagaggc cgtagccata ggaaatgtaa gcataagtgg
     1801 cagtattgac tacgccaaac aaaaaattgc tgatcttgcc cttggccacc aacaccacac
     1861 acatcatgcc cgtcatggca ctgattaagc caatgaggcc actgtcccac ttcacgtata
     1921 aatacacatt gatggcggta aacaccatca accaaatgcg ctcaaaccac gtccactctt
     1981 tgaataactg catgtttttc ctgtctgtaa gcctcaacaa tgtccatcac tgcgcgctcg
     2041 cgctctgcca cactgccgcg tatgtcatgg tatggaattt tgcgccgcgc caaatcggc
//
LOCUS       DAOMVK010000399         2099 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_15619_length_2099_cov_1.955479, whole genome shotgun sequence.
ACCESSION   DAOMVK010000399 DAOMVK010000000
VERSION     DAOMVK010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15619_length_2099_cov_1.955479"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="PK856_10765"
     CDS             complement(<1..517)
                     /locus_tag="PK856_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRL02889.1"
                     /translation="MSSEHTARLLIHCQDQAGIVQAVSGFLYHSGANITSLDQYTTEA
                     VGGDYFMRVEFVLPQLTTQLNSLSDNFTRNVAERYQMNWRISLVQERKKMALLVSKHD
                     HALMELLWRHARGQLPCDIACVISNHEDLCHSVAHFGIPFHVVNVQADTKVAAEAQIN
                     EWVADCDVLVLA"
     assembly_gap    662..671
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <672..856
                     /locus_tag="PK856_10770"
     CDS             <672..856
                     /locus_tag="PK856_10770"
                     /inference="COORDINATES: protein motif:HMM:NF028342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HRL02890.1"
                     /translation="CPICQAASTYQWRSQRSYVSVFFLPLFPVGKERHILQCTQCHTV
                     LPEKFLPTTDANPSRE"
     gene            1099..1743
                     /locus_tag="PK856_10775"
     CDS             1099..1743
                     /locus_tag="PK856_10775"
                     /inference="COORDINATES: protein motif:HMM:NF040019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6198 family protein"
                     /protein_id="HRL02891.1"
                     /translation="MLYVAGLFTLALGCSFSIMANLGVSPVSSLAYSIHLITPWSVGL
                     ASVVVYMAFIAIQMILNRCVDLREACLQMILSVIFGFFMDATLWLLAFLPAAHHWWLQ
                     AAYLIISLWVISCGLVGYFTAKLPLLPYDALSFAIKNRFDIPFSRAKISGDVINVLIS
                     ASLCWGFLHQWGAIGIGTAVAALSIGKIMGFLNPKIQPALLAWLHTHSQQQNAH"
BASE COUNT          526 a          558 c          425 g          580 t
ORIGIN      
        1 gcgccaacac caacacatca caatcggcca cccactcatt gatttgcgcc tcagccgcca
       61 ccttggtatc ggcctgtaca ttgaccacat gaaacggtat gccaaaatgc gctaccgaat
      121 ggcacaaatc ctcatggttg ctgatgacac aggcaatgtc gcacggcaat tgtccacgcg
      181 catgccgcca cagcaattcc atcaaggcat ggtcgtgctt ggacaccagc aacgccattt
      241 tcttgcgctc ttgcaccaag ctgatgcgcc aattcatctg atagcgctca gccacgttgc
      301 gggtgaaatt gtcagacaag ctgttgagct gcgtcgtcaa ttgcggcaac acaaattcaa
      361 cgcgcatgaa gtaatcgcca cccactgcct cggttgtgta ttggtctaaa gacgtgatgt
      421 tggcaccgct gtggtacaaa aatcccgata cggcctgcac gatgcctgct tggtcttggc
      481 aatgaatcaa cagtcttgcg gtgtgttcgc tactcatttt gaaagtgact ttctgcggaa
      541 aacatttcac cctattgtag ccatgctcag gctaaaatgt cgcgtcttca tctgcatttg
      601 gcacccatac tcccatgatt tttatttttg gcatcacgcc caaacaaacc gcatcgaaaa
      661 tnnnnnnnnn ncctgcccca tttgccaagc ggccagtacc taccaatggc gcagtcaacg
      721 ctcttatgtt tcggtctttt ttctgccctt gtttcccgtg ggcaaagagc ggcacatctt
      781 acaatgcaca cagtgccaca ctgtattgcc cgaaaaattc ttgcccacca ccgacgctaa
      841 cccaagccgc gaatgaggat ttgctttgtt gtgtgccgaa attggccaca acaccgtttt
      901 acaggccgca aattcattca ccacccaaac aatttaatat cttcataaaa aataataccc
      961 ctaagcccaa tccaaaccat tttagatata aactcattca attttgaata atatctttta
     1021 aaatcaaaac caatttcgat attattaata gctctaaatt gatataatgt attaatgaaa
     1081 acaatataca aacgattaat gctttatgta gcaggcctgt ttaccttggc cttgggttgc
     1141 agcttttcca tcatggccaa tctgggtgtg tcacccgttt cctctttggc ctattccatc
     1201 catttgatta ccccttggtc ggtgggcttg gccagtgtcg tggtgtacat ggcgtttatt
     1261 gccatccaaa tgattttgaa ccgctgcgtg gatttgcgcg aagcctgcct acaaatgata
     1321 ctgagcgtga tattcggctt ttttatggac gccactttgt ggctgttggc tttcttgcct
     1381 gctgcccacc attggtggtt acaggccgcc tacctcatca tcagcttgtg ggtgatttct
     1441 tgtggtttgg tgggctattt caccgccaaa ctgcctttat tgccttacga cgctttaagc
     1501 ttcgccatca aaaaccgttt cgacattcca ttcagccgcg ccaaaatcag cggcgatgtc
     1561 attaatgtgc tcatttcggc cagcctgtgc tggggctttt tacaccaatg gggcgcgata
     1621 ggcataggca cagcggttgc cgccttgagc ataggcaaaa tcatgggctt tttaaacccc
     1681 aaaatccaac ccgccttact ggcttggtta cacacccact cacagcagca aaacgcccat
     1741 taagcctgtc tttcaggcac tgaatctgat ttccgaacct cccgccagcg gcctgtaaaa
     1801 taattcaggc cgctggtatt ttttcttgta tgattaagac cttacatacc gtgccatatc
     1861 ctgaggctta tttgggcaaa tatgggttta aaaaagcggc cttacaggcc gcattgaatg
     1921 tgtgaataac acaccatttt ttgctcactt ggcatatact ctttagcaac gcaaacaatt
     1981 gccctaaaga agatgctcag gtatggttaa gacctttctt ctggctaaaa tattaacaaa
     2041 tgaaccgcac accattaagc tttgaattct tccctacccg caccgaagaa ggtcggaaa
//
LOCUS       DAOMVK010000400         2096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_15635_length_2096_cov_2.571779, whole genome shotgun sequence.
ACCESSION   DAOMVK010000400 DAOMVK010000000
VERSION     DAOMVK010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15635_length_2096_cov_2.571779"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..817
                     /gene="hpnC"
                     /locus_tag="PK856_10780"
     CDS             2..817
                     /gene="hpnC"
                     /locus_tag="PK856_10780"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HRL02892.1"
                     /translation="MGSVLVPRRLRKPIHAIYAFARTADDYADEGDADNATRLAQLQA
                     LFDELALIEQGKRPVTSLMQRLQDEAIAPFALPTQLFEDLLSAFRQDVVQKRYQEFAG
                     LVDYSRRSANPVGRLLLCLYGVSDEKSLAQSDGICTALQLINFWQDVAVDWHKDRVYL
                     PLQDMAKFGVSEDDIAAQRLTPEFKRLMAYECEKSFKMLQAGSGLVHQLPGRLGWELR
                     LIVLGGQRILQKLDEVGYDVFQHRPELGRKDWWWMLKQLVKPKAKQQRGCHSS"
     gene            835..1170
                     /locus_tag="PK856_10785"
     CDS             835..1170
                     /locus_tag="PK856_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02893.1"
                     /translation="MSTLPKQASNKIPVANKAAPMWPLLLLLLLTPATLLFTALQLPL
                     LNKWPLYALVLWTMALFCYAHRMVYFYHVRKAKKWVYVVAMPCVLALYPSLFMCLLFV
                     AAMEQSVRH"
     gene            1196..1477
                     /locus_tag="PK856_10790"
     CDS             1196..1477
                     /locus_tag="PK856_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02894.1"
                     /translation="MGVYVVWFLLCISGIGLCILTLITANQRMPKVQGVCLLVGVGLL
                     LTTLGLSVYDIQTKPALDTYFMGMQVLPLVMYAWVTYYASKQLRRFKAA"
     gene            complement(1579..1809)
                     /locus_tag="PK856_10795"
     CDS             complement(1579..1809)
                     /locus_tag="PK856_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003746151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NF038104 family lipoprotein"
                     /protein_id="HRL02895.1"
                     /translation="MRKLLLILSTCLMLQGCVYKVVTVPVKAVYTVGKYTVKGTAAVV
                     GAVIPDGDDDKDKKTKHKKSPEQSPESEPTND"
BASE COUNT          435 a          428 c          610 g          623 t
ORIGIN      
        1 tgtgggctcg gtgttggtgc cacggcggtt gcgcaagccc atccatgcca tttatgcgtt
       61 tgcacgcacg gcggacgatt atgccgatga gggcgatgcc gacaatgcca cgcgtttggc
      121 gcaattgcag gccttgtttg atgagttggc tttgattgaa caaggtaagc ggcctgtaac
      181 cagcttgatg cagcgtttgc aagatgaggc gattgcgcca tttgccttgc ccacgcaatt
      241 gtttgaggat ttgctgtcgg catttcgcca agatgtggtg caaaagcgtt atcaggaatt
      301 tgcaggtttg gttgattact cgcgccgctc tgccaatcca gtggggcgct tgctgttgtg
      361 cttgtatggc gtgagcgatg aaaaatcgtt ggcgcaaagc gatggcattt gcacggcttt
      421 acaattgatt aatttttggc aagacgtggc tgtggattgg cacaaagacc gcgtctacct
      481 gcctttgcaa gacatggcca agtttggcgt gagtgaggac gacatcgcag cgcagcgatt
      541 gacgccagaa ttcaagcgct taatggccta tgagtgcgaa aaatcgttta aaatgctgca
      601 agctggctcg ggtttggtgc atcaattgcc agggcgtttg ggttgggaac tgcgtttgat
      661 agtgttgggt gggcagcgga ttttgcaaaa gctggacgaa gtgggctacg atgtgtttca
      721 gcacagacct gaattggggc gcaaagattg gtggtggatg ctcaagcaat tggtcaagcc
      781 gaaagccaaa caacagcgtg gttgtcattc ttcttaaata caagcggcct gtaaatgtcg
      841 acattgccca aacaagcgtc caataagata cctgtggcaa acaaagccgc cccgatgtgg
      901 cctttgctgt tactgctgtt gttgacgcct gccactttgc tgtttacggc cttgcaattg
      961 cctttgctga ataaatggcc gctctatgca ttggtcttgt ggacgatggc cttgttttgt
     1021 tatgcgcacc gcatggtgta tttttaccat gtacgcaagg cgaagaagtg ggtgtatgtg
     1081 gtagccatgc cgtgtgtgct ggctttgtat ccgagtttgt tcatgtgttt gttgtttgtc
     1141 gcggcgatgg agcaaagcgt gcggcactag ttatttggtc acaaaaggaa tggtaatggg
     1201 tgtttatgtg gtgtggtttt tgttgtgtat ttcgggcata ggcttgtgca ttttgacctt
     1261 gataacggcg aatcagcgca tgcctaaggt gcaaggtgtg tgtctgttgg tgggcgtggg
     1321 tttgttgtta acgactttgg gtttgagcgt gtacgacata caaaccaaac cagcgttgga
     1381 cacgtatttt atgggcatgc aggtgttgcc attggtgatg tatgcttggg tcacttatta
     1441 tgccagcaag cagttgcgcc gctttaaagc agcataagtc tttggaatgt tacaggccgc
     1501 ttatctattg gacagataag cggcctgtaa gcattggtag ctgccgtgtt tgatgtgaac
     1561 aaggcttgcg gactgcaatc agtcgtttgt tggttctgat tcaggagatt gctcaggtga
     1621 ttttttgtgt ttggttttct tgtctttgtc atcgtcacca tcgggaatga ctgcacccac
     1681 cacggcggcc gtgcctttga ctgtgtattt gccaacggta tacaccgcct tgacgggaac
     1741 agtcaccact ttgtagacac agccttgtaa catcaggcaa gtacttagaa tcagcaacag
     1801 cttgcgcatg ggatttcctc ggtttgaatg cgctattatc gctgaatttc gcttgctatg
     1861 ttgttaagaa tgtgatttca atagccatga aaaaaccgct gcaagcaacg gttgggttgt
     1921 gtgtttgatt acggctatat caaggctcta tgcgctcagc gcgcttgatg tcaggtttga
     1981 cctcatcaaa ttcgccttca atgatgtcgc catcgctgtg tttgcgttgt tgtttgtagg
     2041 cttgataagc ctgttgggta tgggctgcgg taatgcctgt tttggggccc gccttg
//
LOCUS       DAOMVK010000401         2090 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_15701_length_2090_cov_2.470270, whole genome shotgun sequence.
ACCESSION   DAOMVK010000401 DAOMVK010000000
VERSION     DAOMVK010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15701_length_2090_cov_2.470270"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1709
                     /locus_tag="PK856_10800"
     CDS             <1..1709
                     /locus_tag="PK856_10800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRL02896.1"
                     /translation="KQSYLLLAMVQAKQNQDLPKEITVFETKLAHLTQLMSRSLHADA
                     QSHVPFQAVQAGFVPFKSKILAASQAEQTSPILLAETRAYIATIDELVKGIETENTHS
                     IQIMRMAQGMLMLMVCVSAVLSLLLLNKLVIQPLSKLNQGIQKISDGQLDTRVHIATN
                     DEFHQVSSGFNHMAQRLQDAYQHLEDKVKQKTADLARTNQELSTLYEITAFLHKAPSN
                     KDTMTVFLQKVMALAGAPSGSIRLLNQQGEHMDMTAHINVPPALLANSDCTDAHACLC
                     GDALFQPDFVIYPMATADPNLLCHKLQLDHLTVYQIQLREQTLGLLTLYYDRNEPNPH
                     QELPIITLLCSQLAVSIENNRLGMRETQFAVLEERNIMAQGLHDSIAQSLSFLNLQLQ
                     MLDAAIRKNNNDKIQQHLSFLNTGVQQCYDDVRELLNNFRLKLAHASFQEVLNSVIER
                     FKAQQTTDVTLRYVTDGQDLSPQQQLQLVFIVQEALSNIRKHAQASEVLIDFAHQSDG
                     IKLKIQDNGVGFDTQINKEKQGHHIGLSIMQERIAQIQGQISLESAPGKGTRILVSIH
                     PQ"
     gene            1733..>2090
                     /locus_tag="PK856_10805"
     CDS             1733..>2090
                     /locus_tag="PK856_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009116584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRL02897.1"
                     /translation="MSVINLVLIDDHTLFRTGLRFLLEQEEGFNVIGEATDGLSGIKL
                     VEQLRPNVVLLDLDMPTMTGREALPQLLNSQPDLAVLVLTVSEDAADLTECMRLGARG
                     YLLKNINTEFLLQSIYK"
BASE COUNT          605 a          535 c          448 g          502 t
ORIGIN      
        1 gcaagcaaag ctatttgctg ctggccatgg tacaggccaa gcaaaaccaa gacttgccca
       61 aagaaatcac ggtatttgaa accaaactgg cccatttaac ccaattaatg agccgatcat
      121 tgcatgcaga tgcccaaagt cacgtccctt ttcaggcagt acaggccggc tttgtgcctt
      181 ttaagtcgaa aatattggcg gcctcccaag ccgagcaaac gtcacccata ttgttggctg
      241 aaaccagagc ttacattgcc accatcgatg aattggttaa aggcatcgaa accgaaaaca
      301 cccacagcat ccaaatcatg cgcatggcgc aaggcatgtt aatgttgatg gtgtgtgtct
      361 cagcagtgtt gtctttgctg ttattgaaca aactggtcat acagcctttg tcaaaactca
      421 atcaaggcat ccaaaaaatc agtgatggcc aacttgatac ccgtgttcac atcgccacca
      481 acgatgaatt tcaccaggtt tcttcaggct ttaaccacat ggcccagcgt ttacaggacg
      541 cttaccaaca tttggaagac aaggtcaaac aaaaaaccgc cgacttggcc cgcaccaatc
      601 aagagttatc aaccttgtat gaaatcaccg cttttttgca caaagccccc tcaaacaaag
      661 acaccatgac ggtttttttg caaaaagtga tggcattggc aggcgctccc tctggcagca
      721 tccgtttgct caaccaacaa ggtgagcaca tggacatgac ggcacacatc aacgtgccgc
      781 ccgctttgtt ggcaaactcc gattgcaccg atgcccatgc ctgtttgtgt ggcgatgcct
      841 tgttccaacc cgattttgtc atctacccca tggccaccgc agaccccaat ctgctctgcc
      901 acaagctgca actcgaccac ttgacggtgt accaaatcca attgcgggag caaaccttgg
      961 gtttgttaac gctgtattac gaccgcaacg agcccaatcc tcatcaagaa ttgcccatca
     1021 tcaccttgct gtgtagccaa ttggcggtaa gcatcgaaaa caaccgcttg ggcatgcgtg
     1081 aaacccaatt tgcggtgtta gaagagcgca acatcatggc gcaaggcttg cacgacagca
     1141 ttgcccaatc tttgtctttt ttgaatttgc agttgcaaat gctcgatgcg gccatacgca
     1201 aaaacaacaa cgacaaaatc caacaacacc tgagcttttt aaatacaggc gtacaacagt
     1261 gttatgacga tgtgcgagaa ttgctcaata atttccgctt aaaactggcc catgcttcat
     1321 tccaagaagt gttgaattcc gtcattgagc gctttaaggc acaacaaaca accgacgtaa
     1381 cattgcgcta tgtgactgat ggccaagatt tgagccctca acaacaattg caattggttt
     1441 tcattgtgca agaagccctg tccaacatcc gcaaacatgc ccaagccagc gaagtattga
     1501 ttgacttcgc ccaccaaagc gatggcatca agctgaaaat acaggacaac ggtgtgggtt
     1561 ttgataccca aatcaacaag gaaaaacaag gccatcacat aggcttatcc atcatgcaag
     1621 agcgcattgc acaaattcaa ggacaaattt cgctggagtc tgcaccagga aaaggaaccc
     1681 gcattttggt cagcatccac cctcaataat ttaaagcatt aaaggaactc ccatgtctgt
     1741 catcaatttg gtcttaatag atgaccatac tttatttcga actggcttac gttttttgct
     1801 agaacaagaa gaaggcttta acgtcattgg agaagccacc gatggcttaa gtggcatcaa
     1861 actggttgaa caattgcgcc caaatgtggt gttgttggat ttggacatgc ccacgatgac
     1921 ggggcgagaa gccttgcctc aactgctcaa cagccaacct gacttggcgg tactggtatt
     1981 gacggtttcg gaagacgcgg ccgatttgac cgagtgtatg cgtttggggg ctcgtggata
     2041 tttgctcaaa aacatcaata ccgagttttt actgcaaagc atttacaaag
//
LOCUS       DAOMVK010000402         2088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_15730_length_2088_cov_1.847516, whole genome shotgun sequence.
ACCESSION   DAOMVK010000402 DAOMVK010000000
VERSION     DAOMVK010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15730_length_2088_cov_1.847516"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="PK856_10810"
     CDS             complement(<1..595)
                     /locus_tag="PK856_10810"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRL02898.1"
                     /translation="MKPDQTAYQFDDDMAYFVHWYSVFKPADDDIDSQRATYEAWCHS
                     YTPPMDEDIDVIDTATDCGVALRLYRPQRPAPKQGWPWIVYLHGGMWTYGSLDSHEYI
                     TAPLCKDLNAIVVAVDYSLAPEAAYPDAQRDCRAAIAHVLSQAQAWQLNPQAGVLMGD
                     DAGANLALELGSDLKQPEIKAVVAINPNLETDSKRTEH"
     gene            complement(592..2076)
                     /locus_tag="PK856_10815"
                     /pseudo
     CDS             complement(592..2076)
                     /locus_tag="PK856_10815"
                     /inference="COORDINATES: protein motif:HMM:NF014126.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
BASE COUNT          571 a          547 c          515 g          455 t
ORIGIN      
        1 gatgctcggt gcgcttgctg tctgtttcca aattggggtt gatggcaacc acggctttga
       61 tttcaggttg ttttaagtct gagcccaatt ccaaagccaa gttcgccccc gcatcgtcac
      121 ccatcaacac acctgcttga gggttcaatt gccaagcttg cgcttgactc agcacatgcg
      181 cgatggcggc gcggcaatcg cgttgtgcat cggggtaagc cgcttcaggg gccaaactgt
      241 aatccacggc caccacgatg gcattcaagt ctttgcacaa aggcgcggtg atgtactcat
      301 gcgaatccaa gctgccatac gtccacatgc cgccgtgcaa atacacaatc caaggccagc
      361 cttgttttgg ggcagggcgt tgcggcctgt aaagtcgcaa agccacgccg caatcggttg
      421 ctgtgtcgat gacgtcgatg tcttcgtcca tcggtggtgt gtagctgtga caccatgctt
      481 cgtaagtggc acgctgtgaa tcaatgtcgt cgtccgctgg cttgaacacg ctgtaccagt
      541 gcacaaagta agccatatca tcgtcaaact gataggccgt ttgatcgggt ttcatgactg
      601 atctccattc gatgcggctt tgattgggct caattcgctc acaacaggtg tggcaacggc
      661 ttgctcatcg gcctgtaaac gggcccattc agcggcattg gcttcatcga ttttgtgcgc
      721 agggatgtgg ccatagtctt gttgcaacca cttcaccaag ccataagtct gtaccatgat
      781 gatggcgatg aacggtacgg cggtaatcac ggtggcggct ttcatggtgt ccaaaggcgc
      841 tttggagaaa atcatcgcaa tcggtaccaa ggtcaacatc acacaccaga acaaacgggt
      901 attaggtgac gggtcttggc cttcttccaa accacgggta caggttgccg ataccgcata
      961 gcccaccgca tccatgtgtg ctgccaagaa aaccaccatg atgattaaga agatccacat
     1021 catcagtgtg cccataggca aagtacccaa caattggcct atcgccactt caccgccttg
     1081 tttgctgaga atttcaggca cgttgacgat gttgttgatg aaaccgtgta cgctgaagtt
     1141 ttcaaacacg ccgtacacaa accacatgcc gatacagcct gcaatgatca ccgccaaaat
     1201 cacttcgcgg atgctgcggc ctttggaaac gcgggtcaca aacaaagcca cgccaggggc
     1261 ataagaaatc catcacaacc agtaaaacac cgtccactcg cgggtaaatt tgccatcacc
     1321 aaacgggtcg gcaaacaaac tcatgtcgac aaaattggtt gccatcaagc ctaagctgtt
     1381 gatggtattg ttcatgatga attgggtagg gccaaaaatc agcacataca cagcgaataa
     1441 caccacgcca taacacacaa actggctcaa gcgcttcatg ccatggtcca taccgatgta
     1501 tgagctcaag gcaaacacga tggacaccag caagatgatg accactttgg tggtaaacgt
     1561 attcggtatg cccgtgagca gcgacaagat gttggtaaac gtgaccgcgg ttaacaccaa
     1621 agaaatggtc aaggcaccaa acatgcacaa catgaacagc aaatccacgg cacgaccgac
     1681 cacacctgtg gctttgtaac ctgtcacggc ttccaccata gaggccaagc tcaagccttt
     1741 gtttttgcgc acatggtagt ggtaacacat ggaaatggcg gccaaagcat aaatgctcca
     1801 agcgctgggg ccgtaatgga agaaggaata ggccacactg aatttcagtg catcaggcga
     1861 gctgggtgcg agattcaaac caggcgtttg gtagtaatac gcccagtcca aaaagcccca
     1921 atacaaggca gaagaaccca tgcccgacaa aatgaacatg aaaatccatg tcggggtaga
     1981 gtattcaggc ttgccttcgc ccaatttgat tttgccgtac ttactgaatg ccaaagacaa
     2041 gcaaaaagca atggataaaa aagtgaaaat caacaccggt gctgagaa
//
LOCUS       DAOMVK010000403         2085 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_15747_length_2085_cov_2.520690, whole genome shotgun sequence.
ACCESSION   DAOMVK010000403 DAOMVK010000000
VERSION     DAOMVK010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15747_length_2085_cov_2.520690"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..297)
                     /locus_tag="PK856_10820"
     CDS             complement(109..297)
                     /locus_tag="PK856_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003787631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HRL02899.1"
                     /translation="MSNNEKVITITPHDLPLHCPTADVALWNNHPKVFLPIQSNSTIR
                     CPYCGTVYRLEGEAGGHH"
     gene            complement(471..1409)
                     /locus_tag="PK856_10825"
     CDS             complement(471..1409)
                     /locus_tag="PK856_10825"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HRL02900.1"
                     /translation="MSMSDRDGFIWYNNEMVNWRDANTHVLTHTLHYGMGVFEGVRAY
                     ETAQGPAIFRLQEHTKRLFNSAKIVGMALPYTPEQINQAHIAVVKQNALQSCYFRPMA
                     FYGSKKLGIAPAKDDVNVIVAAWAWGAYLGEDGMNQGIRVKISSFARHHVNVSMVKAK
                     ANGNYMNSIMANQEALNDGYDEAVLLDTDGYVAEGSGENIFMVRDGVLYTPDLSVALA
                     GITRASIITLAQDLGYGVIEKRITRDELYTADELFFTGTAAEVTPIREVDGRSIGNGS
                     RGDITAQLQQAYFDVVQGKDTRYQHWLSYINQNASA"
     gene            1631..1978
                     /locus_tag="PK856_10830"
     CDS             1631..1978
                     /locus_tag="PK856_10830"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HRL02901.1"
                     /translation="MMKWLSIVIMFIVFIALLAVTLGNTHSVDFNLIGLPTTKWPLVV
                     YLWIAFVLGALVGVLAMFGRILRLRNEAARLQKEVKKLRQHNDDLSKQLATYPQATAN
                     AISTADVIVPVQP"
BASE COUNT          499 a          469 c          533 g          584 t
ORIGIN      
        1 ggtatcagcc tggcggtgtt tgaggcgttg gagcagaggc tgcgtcatca cgcaatcgcc
       61 tatccaagag ggcgcgatga ttaaaatgcg ataactcatg ggcggcgcct agtgatgacc
      121 gccagcttcg ccttctaaac ggtaaacagt gccgcagtaa gggcagcgga tggtgctgtt
      181 ggattgaatt ggcaaaaaca ctttaggatg gttgttccac aaagccacat cggcagttgg
      241 gcagtgcaaa ggcaaatcat gaggagtaat ggtgatgact ttttcgttat tgctcataaa
      301 ggtgtttcct gtttaagttt catttttgca cccaagtggg tggtattgcc acgcgacagt
      361 tgtaactgtt ttgcgcgtga aatcatgtgg cttgccgtat tgaggttctt aatataccct
      421 gtacacaagc ttacaggccg ctattttact gcttttatgc tcaacctgca ttatgcgctt
      481 gcattctgat tgatgtagct taaccagtgt tggtagcgag tgtctttgcc ttgtaccaca
      541 tcgaaatagg cttgctgcaa ttgtgccgtg atatcgccgc ggctgccatt gcctatgctg
      601 cggccatcga cttcgcgtat gggcgtgact tctgcggcag tgcccgtgaa aaacaattcg
      661 tctgcggtat acaattcatc gcgggtgatg cgcttttcaa tcacgccata gcccaagtct
      721 tgtgccaaag tgatgatgct ggcgcgggta atgcctgcta aggccacgct caggtcggga
      781 gtataaagca caccgtcgcg caccatgaaa atgttttctc ccgagccttc ggccacatag
      841 ccatcggtat cgagcaaaac agcttcatca tagccatcgt ttaaggcctc ttgattggcc
      901 atgatggaat tcatgtaatt gccattggcc ttggctttga ccatgctcac attgacatgg
      961 tggcgggcaa acgatgaaat tttcacccgt atgccttggt tcatgccatc ttcacccaaa
     1021 taagcgcccc aagcccaagc cgccacgatc acgttcacat cgtctttggc gggcgcgatg
     1081 cccagttttt tcgagccata aaatgccatg ggtcggaaat aacacgattg caatgcgttt
     1141 tgttttacca ccgcaatgtg agcttggttg atttgttctg gggtgtaagg caaagccatg
     1201 ccgactattt tggccgagtt gaacaagcgt ttggtgtgtt cttgcaaacg gaaaatggca
     1261 gggccttggg ctgtttcata cgcacgcacg ccttcaaaca cacccatacc ataatgcagt
     1321 gtgtgggtta acacatgtgt attggcatcg cgccagttca ccatttcgtt gttataccaa
     1381 ataaagccat cacggtctga catgctcata aggcactcct taatagagat tgggttaggg
     1441 cagaacaaca tagagataca caacgtaagt ggcgcaaatt acacattgcg tgtgttcatg
     1501 gcacacagac gacatattct tggtacagtt taaggcataa acccatacca tcaaagcgtt
     1561 tggtgatacc ttaaaccatg aaaatcgtat aaactgcagg gcaaatcgaa ttcaaaaaag
     1621 gaccaggtgc atgatgaagt ggctctctat tgtcattatg ttcatcgtat tcattgcctt
     1681 attggcagtg actttgggca atacccacag tgtggatttt aatttaattg gtctgcctac
     1741 cacgaaatgg cctttggttg tttatttgtg gattgcgttt gtcttgggtg cattggttgg
     1801 ggtattggcc atgtttggtc gtattttgcg tttgcgtaat gaggctgccc gtttgcaaaa
     1861 agaagtgaaa aaactgcgcc aacacaacga tgacttgagc aaacaattgg ccacttaccc
     1921 acaagcaact gccaatgcca tcagtactgc tgatgtgatt gtgcctgtgc agccgtaaga
     1981 aacggactgc agatggatac agttgtatgg tggtgggtgt tacccgtgtg tatgttgccc
     2041 gtgttttttg ccatgggttg gtttgctgcc cgtgtggaca tgcgg
//
LOCUS       DAOMVK010000404         2080 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_15799_length_2080_cov_2.845432, whole genome shotgun sequence.
ACCESSION   DAOMVK010000404 DAOMVK010000000
VERSION     DAOMVK010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15799_length_2080_cov_2.845432"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PK856_10835"
     CDS             complement(<1..446)
                     /locus_tag="PK856_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRL02902.1"
                     /translation="MTEVVIVAAKRTAIGNFGGGLSGLVAPELGAAVIKALLEETGVK
                     AVDEVILGEVLTAGVGQNPARQAALKAGLGIETPCLIINQVCGSGLKVTHLGAQAIKA
                     GDADIIIAGGQESMSNSPYLLPQARNGYRMGDGKVVDSMVADGLTDA"
     gene            complement(473..>2080)
                     /locus_tag="PK856_10840"
     CDS             complement(473..>2080)
                     /locus_tag="PK856_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRL02903.1"
                     /translation="TQAQDSTNVWQQMLTQQTEMWQKNLASVFSLPNNHIPSATEQWQ
                     NLPFFQNVQKMYAQTCDFVESQLQSNPAYSRLSAEQQDNMAFMTRQYLSAMNPNNYLM
                     TNPEALQEAFQTQGQSLVKGMQNYLTDLEKGRITMSDESQFVLGQNIAATPGKVVLKN
                     ELIELLHYKPTAEKVYAKPLLIVPPCVNKYYLMDLGPQKSLVEYYVEQGYNVFLISWR
                     SAVEETKHFTWDTYVERGVISAVSAITSISKQDSINALGFCIGGLILSTAVCVMKARG
                     EQRIDNAILMTSMADHTEPGDIKYFLSEDVVRMREGKMAQGGIISGLELQATFSALRP
                     DDLVWNYVQNNYLKGKTPTSFDLLYWNNDSVDLPLPMHTFFLRQFYLNNALAKPGSMS
                     VCGVPIDLRNIDIPLYFFAAEGDHIVPWLAVYNGLPLYGNAQERRFVLGESGHIAGAI
                     NPVTKDRRSYWTGNTEGKTAEEWRATAENHKGSWWKDLNTWLSSRSGNLVNAPKTLGN
                     TSHKPQADAPGDYVRAQAMNVVKAHLV"
BASE COUNT          524 a          496 c          487 g          573 t
ORIGIN      
        1 gcatcggtca aaccgtcggc aaccatggag tcaaccactt tgccatcgcc catgcggtag
       61 ccattgcgcg cttgtggcaa caaataaggt gagttagaca tagactcttg gccaccagcg
      121 atgatgatgt ctgcatcgcc tgctttgatc gcttgcgcgc ccaaatgggt cactttcaag
      181 ccagaaccac acacttggtt gatgatgagg caaggggttt cgatgcccaa gcctgctttt
      241 aaggccgctt gacgggcagg gttttgaccc acaccagcag tcaaaacttc acccaaaatc
      301 acttcgtcta cggcttttac gccagtctct tctaacaacg ctttgatgac tgctgcaccc
      361 aattcaggcg caaccaaacc agacaaaccc ccaccgaaat taccaattgc ggtacgcttt
      421 gctgcaacga ttacaacttc tgtcatattg ttctccatta ttatattggg tattaaacca
      481 agtgagcttt gaccacattc atggcttgtg cgcgcacata atcgcctggt gcatcagctt
      541 gtggcttgtg cgatgtattg cctaaggttt taggcgcatt caccaagtta ccagaacggc
      601 tagaaagcca agtattcaaa tccttccacc agctgccttt gtggttttca gcagtggcac
      661 gccactcttc ggcggttttg ccttcggtat taccagtcca atagctgcgg cggtctttgg
      721 taacgggatt aatcgcgcca gcaatgtggc cagattcacc cagtacaaaa cgacgctctt
      781 gtgcattgcc atacaaaggc aagccattgt aaaccgccaa ccaaggcaca atgtggtcac
      841 cctctgctgc aaagaagtat agcgggatat caatattacg caagtctatt ggcacgccac
      901 acacgctcat gctgcctggt ttggctaagg cattgttcaa atagaactgg cgcaagaaga
      961 aggtgtgcat aggcaatggt aaatcgactg aatcattgtt ccagtacagc aagtcaaatg
     1021 aggttggtgt tttgcctttt aagtagttgt tttgaacata attccacacc aagtcgtcag
     1081 gacgtaaggc agagaaagtg gcctgtaatt ccaagccgct gatgatgccg ccttgggcca
     1141 ttttgccttc gcgcatgcgc accacatctt cagacaagaa gtatttgatg tcaccaggtt
     1201 cagtatgatc agccatagac gtcatcaaaa tggcattgtc tatgcgttgc tcaccacgtg
     1261 ctttcatgac acacactgca gtagacaaaa tcaaaccacc gatacagaag cctaaggcat
     1321 tgatgctgtc ttgtttgctg atgctggtga tggcactgac tgcactgatg acaccacgct
     1381 cgacataggt atcccaagtg aagtgcttgg tttcttcaac tgcactgcgc catgaaatca
     1441 agaagacgtt atagccttgt tccacatagt attccaccaa ggatttttgc gggcccaaat
     1501 ccatcaaata gtatttgttc acacacggtg gcacgatgag caaaggcttg gcatacactt
     1561 tttctgcagt gggtttgtaa tgcagcaatt caatcaattc attttttaac accaccttgc
     1621 ctggggttgc cgcaatgttt tgacccaaaa caaattggct ttcatctgac atggtgatgc
     1681 ggcctttttc caagtcagtc aggtagtttt gcatgccttt aaccagactt tggccttggg
     1741 tttgaaaagc ttcttgtaag gcttcagggt tggtcatcag gtagttgtta ggattcatgg
     1801 ccgacaggta ttgacgggtc atgaaggcca tattgtcttg ttgctcggcg ctcaagcgcg
     1861 agtaagcagg gttgctttgc aattggcttt caacaaaatc acaggtttga gcatacattt
     1921 tctgaacatt ttggaagaaa ggcagatttt gccattgttc agtggctgaa ggaatgtggt
     1981 tgtttggcaa tgaaaaaaca gaagccaaat ttttttgcca catctctgtt tgttgggtga
     2041 gcatttgttg ccagacattt gtgctgtctt gggcttgggt
//
LOCUS       DAOMVK010000405         2077 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_15829_length_2077_cov_1.971316, whole genome shotgun sequence.
ACCESSION   DAOMVK010000405 DAOMVK010000000
VERSION     DAOMVK010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15829_length_2077_cov_1.971316"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..>1636)
                     /locus_tag="PK856_10845"
     CDS             complement(97..>1636)
                     /locus_tag="PK856_10845"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRL02904.1"
                     /translation="LCVNLLVPSNENWATLSQYWLKQYITNTLILVTGVGLVVAALGS
                     GLAFLMSYFQFPLKKFFEFLLVLPLAIPAYISAYTYSEMLSYTGVVQKTLRNVFDLKL
                     PATYFDIMSLPGAVFIFAMALFPYVYLMVRSFLLRQDAGLMESARLLGKNVFTAYLTV
                     IMPMTLPVMVASATLACLEVLSDYGVTSHFGIHTFSTGIFQAWFGMHDVFTAIKVAFC
                     GMLIASSLIVINRVFTNTKKVQSQSSGKPIQPMPLHGASLWLAMGVLGAVALLSSIIP
                     IAQMLVWAVQTLMHQNELQGLWENTQNTVMVAFLAAVAVIVFALVVCNVARYFSAFWI
                     KMPMWVGGFGYSFPSPVLAIGVLGLLISLDKWLHSMQWVNNPLLLTSSLFTLVFAYVI
                     KYAAVGINGIDSGFVRVGKRYSEASRTLGQGMTATFFKVDVFTIKHSIIAAFILVFID
                     IVKELPMTLLLRPYNFSTLATRVYTYAGDEQIQRASVPSLVIILICVVLMLVVNRLEK
                     NRHY"
     assembly_gap    1637..1646
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1701..>2077)
                     /locus_tag="PK856_10850"
     CDS             complement(1701..>2077)
                     /locus_tag="PK856_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HRL02905.1"
                     /translation="IMNTYYVGLMANSTDPEEKKIGESLGVFFPNQATTGTHINISGA
                     GLTKSSKNKANATKLLEYLSDVPAQEIVSNENYEFPVNPKAKPAELLRSWGTFKTQQI
                     SFAELGKNNAAAIALMGKTGWK"
BASE COUNT          606 a          512 c          445 g          504 t
ORIGIN      
        1 caatcgcctt tttgtatcga aagattaaac ccttgcagcg tgggtttttc actcttggga
       61 taggaaaaac caatgttttc aagtgagagg tacatatcaa taatgcctgt tcttttctaa
      121 tctgttcaca accaacatca acaccacgca aatcaagatg atgaccaatg aaggcacaga
      181 agcccgttga atttgctcgt cacccgcgta ggtatacacc cgtgtggcca aggtggaaaa
      241 attataaggc ctgagcaaca aagtcattgg cagctctttc acaatgtcga tgaacaccaa
      301 gataaacgcg gcgatgatgg aatgcttaat cgtaaacaca tcgactttga aaaaagtggc
      361 cgtcatgcct tgccccaatg tgcgcgaggc ttctgagtag cgcttgccca cgcgcacaaa
      421 gccgctgtct atgccattga tgcccactgc cgcgtacttg atgacatagg caaacaccaa
      481 ggtaaacaaa gaagaagtga gcaacaaagg attgttgacc cattgcatgc tgtgcagcca
      541 tttgtccagc gagattaaca aacccaatac cccaatggcc aacacaggcg atgggaatga
      601 gtagccaaaa ccgcctaccc acatcggcat cttgatccaa aatgccgaaa aataccgcgc
      661 cacattacac accaccaatg caaacacaat cacagcaaca gcagccaaaa acgctaccat
      721 gacggtgttt tgggtgtttt cccacaagcc ttgcaattca ttttggtgca tcagtgtttg
      781 tacagcccac accagcattt gggctatggg tatgatgctg gacagcaagg ccaccgcacc
      841 taacactccc atagccagcc acaaacttgc accatgcaat ggcatgggtt gtatcggttt
      901 gccactgctt tggctttgta cttttttggt gttggtaaac acccgattga tgacgattaa
      961 gctgctggca atgagcatgc cgcaaaacgc cactttaatc gcggtgaata catcgtgcat
     1021 gccaaaccaa gcttggaaaa tacctgtaga gaaagtgtgt atgccaaaat gactggtgac
     1081 accgtaatcg ctcaatacct ctaaacaagc caaagtggca ctggccacca tcacaggcaa
     1141 agtcatgggc atgatcaccg tcagataggc agtgaacaca tttttgccca acaggcgcgc
     1201 actttccatc agtcccgcgt cttgtctgag caaaaagctg cgcaccatca aatacacata
     1261 cggaaacaaa gccatggcaa aaatgaatac cgcaccaggc aaagacatga tgtcgaaata
     1321 cgttgcgggc aatttcaaat caaacacatt gcgcagtgtt ttttgcacca cccctgtgta
     1381 gctgagcatt tcgctgtaag tgtatgccga aatgtaagca ggtatcgcca agggcaatac
     1441 caataaaaat tcaaaaaact ttttcaacgg aaattgaaaa taactcatta aaaatgccaa
     1501 gccactgccc agcgcagcca ccacaaggcc gactcctgtt accaaaatca aagtattggt
     1561 gatgtattgt ttcaaccagt actgtgagag cgtggcccaa ttttcattgg atggcaccaa
     1621 cagattcaca cacaaannnn nnnnnntcct ccgatttgac tgagcgtgat ccaaagacga
     1681 ttggatttat tcagcatgca ttatttccag ccagttttac ccatcaacgc aatggcagcc
     1741 gcattgtttt tgcccaattc agcaaaactg atttgttggg ttttgaatgt gccccatgag
     1801 cgcaacaatt cagcaggctt ggctttagga ttcacaggga attcatagtt ttcattggaa
     1861 acgatttctt gcgcaggcac atcgcttaag tattccagca atttggtggc attggctttg
     1921 tttttgctcg acttggtcaa tccagcgcca ctgatgttga tgtgggtgcc tgtggtggct
     1981 tgattcggga agaacacgcc caaactttca ccgatttttt tctcttcagg gtcggtagaa
     2041 tttgccatca agcccacata ataggtattc atgatgg
//
LOCUS       DAOMVK010000406         2076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_15836_length_2076_cov_2.984166, whole genome shotgun sequence.
ACCESSION   DAOMVK010000406 DAOMVK010000000
VERSION     DAOMVK010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15836_length_2076_cov_2.984166"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1591)
                     /gene="pgi"
                     /locus_tag="PK856_10855"
     CDS             complement(<1..1591)
                     /gene="pgi"
                     /locus_tag="PK856_10855"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HRL02906.1"
                     /translation="MLGHDITQTHAWAQLFAHHNEIGDKHMREWFEQDSERFAKMHEH
                     LDGMLVDYSKNRITEESLQLLTELADAADLAQWRERMFTGDKINFSEHRAVLHTALRQ
                     PQDAVIEVDGVNVVAEAYAALDEALDFAELVRSGAHLGHSGERIRHIINIGIGGSDLG
                     PKMVVEALQAYASSEMQVRFVSNVDGANLSQVLQGMRPETTLFIVASKSFTTPETLLN
                     AQAAKDWLLAQTGWNETAIAQHFVAVSTNLQATAQFGIANQCVFKMFDWVGGRYSVWS
                     TIGLPVMCAIGRDEFMRFLTGARAMDEHFLNQPWRHNVPVLLALLGIWYNNFFDAHSH
                     AVIPYDYGMRALTEYLQQLDMESNGKNVTQENRPVECATGPIIWGSQGVNCQHAYFQM
                     LHQGSRLIPSDFIVPMNSHYPLGRQHQVLVANAFAQTEALMRGKTLAEAETELLQAGK
                     GTQLAAQKVFSGNQPTTSILIDKVTPHSLGMLIAMYEHKVFVQGVIWGINSFDQWGVE
                     YGKVLAKTIEPELTGRSDADHD"
     gene            complement(1593..>2076)
                     /gene="orn"
                     /locus_tag="PK856_10860"
     CDS             complement(1593..>2076)
                     /gene="orn"
                     /locus_tag="PK856_10860"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009115660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HRL02907.1"
                     /translation="RIIEAAMIVTDTNLNILAQSPVWVIHQSDALLAAMDEWNTATHT
                     RTGLVERVKASDLSEADAEAQMLAFMSQYVPEKTSPMCGNSVHQDRRFMQKYMPQLEA
                     YFHYRNLDVSTLKELAKRWNPEVAKGVVKKGAHQALADIEESIEEMRHYRDHFLRLKD
                     "
BASE COUNT          524 a          566 c          443 g          543 t
ORIGIN      
        1 catcatggtc tgcatcactc cgccctgtta attctggttc tatggttttg gccaatactt
       61 tgccgtattc cacaccccat tgatcgaatg aattgatgcc ccaaatcacc ccttgcacaa
      121 acactttgtg ctcgtacatg gcaatgagca tacccaaact gtgtggcgtc accttgtcaa
      181 tgagaattga agtagtgggt tgattgcccg aaaagacttt ttgtgctgcc aattgtgtgc
      241 ctttgccggc ctgtaacaac tctgtctcag cttcagccaa ggttttacca cgcattaagg
      301 cctctgtttg cgcaaaggca ttggccacca agacttggtg ttgtcgcccc aaaggataat
      361 gactgttcat aggcacaata aagtcgctgg gtatcaaacg tgagccttga tgcaacattt
      421 gaaaatacgc atgctggcaa ttcacccctt gactccccca aataatgggg ccagtggcac
      481 attctactgg acggttctct tgggtgacat ttttgccatt gctttccatg tcaagctgct
      541 gcagatactc ggttaaagcc cgcatgccgt agtcataagg tatcaccgca tggctgtggg
      601 catcgaaaaa gttgttatac caaatgccca gcaaggccaa caacacgggc acgttgtgac
      661 gccaaggctg attcaagaag tgctcatcca ttgcccgagc acctgtcaag aaccgcataa
      721 attcatcacg acctatggca cacatcacgg gcaaaccgat ggtagaccac accgaataac
      781 gcccacccac ccaatcaaac attttaaaca cacattgatt ggctatacca aattgagccg
      841 tggcctgtaa gtttgtcgac accgccacaa aatgttgtgc aatagcagtc tcattccaac
      901 ccgtttgtgc caacagccaa tccttggctg cttgagcatt gagcaaggtt tctggggtag
      961 taaacgattt gctggccaca ataaacaaag tggtttcagg ccgcattcct tgtaaaacct
     1021 gcgacaaatt ggcaccgtcc acattcgata caaaccgcac ttgcatctct gagctggcat
     1081 aagcctgtaa agcctctacc accattttgg gccccaagtc tgaaccacct atgccaatat
     1141 tgataatgtg gcgtatgcgt tcaccactgt gacccaagtg cgccccactg cgcaccaact
     1201 cggcaaaatc taaggcttca tccaatgccg cataagcctc tgccaccaca ttgacaccat
     1261 ccacctcaat cacagcatcc tgcggttgac gtaaagccgt gtgcaacacc gccctatgtt
     1321 cactgaaatt gattttgtcg ccagtgaaca tgcgttcgcg ccactgagcc aaatctgctg
     1381 catcagccaa ttcagtaagc agctgcaaac tctcctcagt gatgcgattt ttactgtaat
     1441 caaccaacat cccgtccaag tgctcatgca ttttggcaaa acgctcactg tcttgctcaa
     1501 accactcacg catgtgtttg tcacctatct cattatggtg agcaaacaat tgcgcccaag
     1561 catgggtttg agtgatgtcg tgtccaagca tgctaatcct ttaaacgcaa gaagtggtca
     1621 cggtaatggc gcatttcttc tatgctctct tcaatgtctg ccaaagcttg atgcgcccct
     1681 tttttcacca cacctttggc cacttcaggg ttccagcgtt tggccaattc ttttaaggta
     1741 gacacgtcca aattgcggta atggaaatag gcttccaatt gcggcatgta tttttgcatg
     1801 aagcggcggt cttggtgcac cgaatttcca cacatgggcg aagttttttc aggaacatat
     1861 tgcgacatga aggccaacat ttgtgcttct gcatctgctt cgctcaaatc agatgctttc
     1921 acgcgctcta ccaagcctgt gcgggtgtga gtggcggtat tccattcatc catcgccgcc
     1981 aataaagcat cgctttggtg tatcacccaa acaggcgatt gcgccaagat gtttaaattg
     2041 gtgtcggtca ctatcatggc ggcttcgata atgcgc
//
LOCUS       DAOMVK010000407         2063 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_15975_length_2063_cov_2.453685, whole genome shotgun sequence.
ACCESSION   DAOMVK010000407 DAOMVK010000000
VERSION     DAOMVK010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15975_length_2063_cov_2.453685"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /gene="prmA"
                     /locus_tag="PK856_10865"
     CDS             <1..662
                     /gene="prmA"
                     /locus_tag="PK856_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003787600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRL02908.1"
                     /translation="LLPENMRPVKEAYTVTEVADQDWVKLTQSQFDPIHVSDRLWITP
                     TWHEAPTDGSINIELDPGLAFGTGSHPTTFLCLRWLDAHMPQGASVLDYGCGSGILAI
                     TAAKLGAGKVVGVDIDKQAIRASHDNATQNQVNIEVYLPDAQPEGQFDVVVANILANP
                     LRMLGQMLAGRVKTDGQIVLSGILAEQVEEISALYQQWFDMKPATIQDGWACLFGTKR
                     A"
     gene            753..>1432
                     /locus_tag="PK856_10870"
     CDS             753..>1432
                     /locus_tag="PK856_10870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02909.1"
                     /translation="MTETSQVVYHAQCPHCATIRKISHAQLQAAKGLVQCQQCDQTYP
                     ARGNLLKPDQIKQLAEQTADTPSATHIAFDFTANPAKADATSATTESLHSDTAALDDI
                     FERLAADARSETKASAPSVDDVASVEDELADAPKKAKSSGLFAKLLSRKNQHQDIEED
                     DSDVIFETVDKEDGFLNNKAQLHVGADVRPQGVLMEHISAADSEEMVRKYRENKNAAK
                     KRPVNETTT"
     assembly_gap    1433..1442
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1443..>1672
                     /locus_tag="PK856_10875"
     CDS             <1443..>1672
                     /locus_tag="PK856_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02910.1"
                     /translation="ALLAVLKDLVESQPDQTQQTVKAKAPSGILAEQVNTINHADDFV
                     FQMQEEVDDNITQAPTDKASVKTSNHSKLQTL"
     assembly_gap    1673..1733
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1734..>2063
                     /locus_tag="PK856_10880"
     CDS             <1734..>2063
                     /locus_tag="PK856_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02911.1"
                     /translation="DYDANKAWFTQFFNSLNQNQGMSILGQTEADNDTVTLDENGEPI
                     PAAKRMHETGSNTVAMRLPENSMLVFTLEDGGTSHSQLSFIDIQEHLPHKHPEPSHTT
                     TIVQQSH"
BASE COUNT          578 a          508 c          460 g          446 t
ORIGIN      
        1 ccttgttgcc agaaaacatg cggcctgtaa aagaagcgta taccgtgact gaagtggcag
       61 accaagactg ggtgaaactc acccaatcgc agtttgaccc aatacatgta tcggataggc
      121 tgtggattac cccaacttgg catgaagcac cgactgatgg ttcgattaac atcgaattag
      181 acccaggttt ggcatttggc acaggcagcc accccaccac cttcttgtgc ttgcgttggc
      241 tcgatgccca tatgccacaa ggtgcgtcgg tgttggatta tggctgtggc tcgggcattt
      301 tggcgattac tgcggcgaaa ttgggtgcag gcaaagtggt tggtgtggac atagacaaac
      361 aagccatccg tgccagtcac gacaatgcca cacaaaacca agtgaatatt gaagtgtatt
      421 tgccagatgc gcagccagaa ggccaatttg atgtggtggt ggcgaatatc ttggccaacc
      481 cactgcgcat gctgggtcaa atgttggcgg ggcgtgttaa aaccgatggt caaatcgtac
      541 tgtctggtat attggccgag caagtagagg aaatctctgc tttgtatcaa caatggtttg
      601 acatgaaacc tgctaccatc caagacggct gggcctgctt atttggtact aaacgtgctt
      661 aaataactat tgtcctgtgg ttaacctgtg ttaggcttat gcctcgtgca ttcgctgcac
      721 gggttaacct ttattttgtc tttaggtgag ttatgaccga aacgtcacaa gtggtttatc
      781 acgcacagtg cccacactgc gcaactattc gtaaaatcag tcatgcacag ttacaggccg
      841 ctaaaggctt ggtgcaatgc caacaatgtg atcaaaccta cccagcacgt ggtaacttac
      901 tgaagcctga tcaaatcaaa cagcttgctg agcaaactgc tgacacgcca agcgccaccc
      961 acattgcatt tgactttacc gccaatcctg caaaagccga tgccacctca gccacaactg
     1021 aatccctgca ttccgacact gctgccttag acgatatttt tgaacgtctg gccgcggatg
     1081 cccgcagcga aaccaaagca tcagccccat cagttgatga tgttgcctca gttgaagatg
     1141 agcttgctga cgcgcccaaa aaagccaaaa gcagcggtct gtttgccaaa ttactgtcgc
     1201 gtaaaaacca acaccaagac atcgaagaag atgacagcga cgttattttt gaaaccgttg
     1261 acaaagaaga cggctttttg aataacaaag cccagttgca tgtgggtgcc gatgttcgcc
     1321 cacaaggtgt gttaatggaa cacatcagtg cagctgacag cgaagaaatg gtccggaaat
     1381 accgtgaaaa caaaaatgca gcaaaaaagc ggcctgtaaa cgaaaccacc acnnnnnnnn
     1441 nngccttact cgccgtatta aaagacttgg ttgaatcaca gcccgaccaa acacaacaaa
     1501 ctgtgaaagc caaagctccc tcaggcatac ttgctgagca ggttaacacc atcaaccatg
     1561 ccgatgactt tgtgttccaa atgcaagaag aagttgacga caacattacc caagcgccaa
     1621 cggataaagc ctcagttaaa accagcaatc acagcaaatt gcaaacgctg ttnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgattac
     1741 gatgccaaca aggcttggtt tacccaattt ttcaacagcc tcaatcaaaa tcaaggcatg
     1801 tcaatcttgg gtcaaacgga agccgacaat gacacggtga ccttggacga aaatggtgag
     1861 cccattcctg ctgccaagcg catgcatgaa acaggcagca atacggttgc catgcgcttg
     1921 cctgaaaaca gcatgctggt gtttacttta gaagatggtg gcaccagcca cagccaattg
     1981 tcgtttatcg acatacaaga acatttgccc cacaagcatc ctgagcccag ccacaccacc
     2041 accattgtgc agcaaagcca cca
//
LOCUS       DAOMVK010000408         2060 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_16004_length_2060_cov_2.828928, whole genome shotgun sequence.
ACCESSION   DAOMVK010000408 DAOMVK010000000
VERSION     DAOMVK010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16004_length_2060_cov_2.828928"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>437
                     /locus_tag="PK856_10885"
     CDS             <1..>437
                     /locus_tag="PK856_10885"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRL02912.1"
                     /translation="GASGLWQFMPATGKQYGLEQNHVYDGRHDVYASTDAALNYLEYL
                     YGLFGDWSLALAAYNWGEGNVARAVKRTESQGLLPTYENLKMPQETRNYAPKLLAWRN
                     IVSNPAAFGINIPKIEDDEYFEALNVTTPIDTAQAARLAGISQ"
     assembly_gap    438..447
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <448..1834
                     /locus_tag="PK856_10890"
     CDS             <448..1834
                     /locus_tag="PK856_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRL02913.1"
                     /translation="FIPTAKRKMLLPKNAVNHFVRNYQSSDVNQLLSYNIYTPMSGEK
                     VQDVAASFGMSDSELKQINGIRNNVLSSGIPVLVAKNRQPVSNAAGDFNQANFTGDSN
                     ATFAAVAVTTRQADALSKPKAQIAEAAPLKIRASRAKPLIQQNFVAKSEIRSFTEQED
                     DALGKTALAAVEKHATAPTSSARPVNTPMQLANDVVKTQPVTTPRVSSFNNVQAASTQ
                     LVVNTVKTETIQATPANTGIADNAANTSVVNSFAEQATRSPAINTAAIKPQVVVETDP
                     LLALMNKGNNVAAPVAVTHTPSSEIAQQNTVDANFAANAVREAIAREDAKEAREMERL
                     AKIRNENEKRERQLANATQHKVNSGDTLYSIAKTYDMTMSELLAANGLNDNNIKVGQT
                     LVVGKAADRAIQTAKAKEQKQQADMNYVVRKGDTLNSIAGKFNLKVNDLRKWNNGNNI
                     RPGQKIQLRQL"
BASE COUNT          610 a          542 c          451 g          447 t
ORIGIN      
        1 ggggcatctg gtttgtggca attcatgcca gcaacaggca aacaatatgg cttggaacaa
       61 aaccatgttt acgatggccg tcacgacgtt tatgcgtcca ccgatgctgc gttgaactat
      121 ttggaatacc tgtatggctt gtttggcgac tggtctttgg ccttggctgc gtataactgg
      181 ggtgaaggca atgtggcccg cgcagtcaaa cgcaccgaaa gccaaggctt attgccaact
      241 tatgaaaacc tcaaaatgcc gcaagaaacg cgcaattacg cccctaaact gttggcatgg
      301 cgcaatatcg tttccaatcc tgcggctttt ggcatcaaca ttcccaaaat cgaagacgat
      361 gaatacttcg aggctttgaa tgtgaccacg cccattgaca ctgcccaagc agctcgcttg
      421 gcaggcatca gccaagannn nnnnnnngtt cattcctaca gccaaacgca aaatgttgct
      481 acccaaaaat gcggtcaacc attttgtccg caattaccag tcttcagatg tgaatcagct
      541 gctgtcttac aatatttaca ccccgatgag cggtgaaaaa gtccaagacg tggccgccag
      601 ctttggcatg tcagacagtg aattgaagca aatcaatggc atacgcaaca atgttttaag
      661 ctctggcatt cccgttttgg tggccaaaaa ccgtcagcct gtttccaatg ctgctggcga
      721 ttttaaccaa gccaacttta caggcgacag caacgcgact tttgctgcgg tggctgtgac
      781 aacccgtcaa gccgatgcac tgtctaaacc caaggcccaa atcgctgaag ctgcgccatt
      841 gaaaattcgt gccagccgcg ccaagccatt gattcagcaa aacttcgttg ccaaatctga
      901 aatccgcagc ttcactgaac aagaagatga tgcattgggc aaaaccgctt tggctgcagt
      961 agaaaaacac gccactgcac caacatcgtc tgcacggcct gtaaacacac ccatgcaatt
     1021 ggccaacgac gtggttaaaa ctcaaccggt taccacacct cgtgtgagca gcttcaataa
     1081 tgtacaggcc gcatcgacac agttagtagt gaacactgta aaaaccgaaa ccatacaagc
     1141 cacacctgcc aacaccggca ttgctgacaa tgctgcaaac acctcggtag tgaacagttt
     1201 tgcggaacag gccacacgca gccctgccat caataccgcc gcaatcaaac cacaagtcgt
     1261 ggttgaaacc gacccgttat tggctttaat gaacaaaggc aataatgtag cggctcctgt
     1321 ggctgttacc cacacgccta gcagtgaaat tgcccaacaa aataccgttg atgccaattt
     1381 tgccgccaat gctgtacgcg aagcgattgc gcgcgaagac gccaaagaag cccgcgaaat
     1441 ggaacgcttg gccaaaatac gcaacgaaaa cgaaaaacgt gagcgtcaat tggccaacgc
     1501 cacccaacac aaagtcaatt cgggtgacac tttgtacagt atcgccaaaa cctacgacat
     1561 gaccatgtct gaactcttag ccgccaatgg cttgaacgac aacaacatca aagtgggcca
     1621 aactttggtg gttggtaaag ctgctgaccg tgccattcaa acggccaaag ccaaagaaca
     1681 aaagcaacaa gctgacatga attatgttgt gcgcaaaggc gatacgctca acagcattgc
     1741 aggcaaattc aacctcaaag tgaatgattt acgcaaatgg aataacggaa acaacatccg
     1801 tccaggacaa aaaatccagt tgcgtcaact ataagctgca ctgcaacaca aaaagaacag
     1861 ctgaaaagct gttttttttt gcaatattga ctacactgcc gatgacgctg atgacacact
     1921 cagcgtcgtt ctttgaatgt atatgaccca gatacatgcc tacaatcagg caatgttctt
     1981 gctaaggtgc acagtatgat cagcgtattc gatattttta aagtgggctt gggcccatcc
     2041 agttcccata ccgttggccc
//
LOCUS       DAOMVK010000409         2059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_16018_length_2059_cov_2.394212, whole genome shotgun sequence.
ACCESSION   DAOMVK010000409 DAOMVK010000000
VERSION     DAOMVK010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16018_length_2059_cov_2.394212"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /gene="modA"
                     /locus_tag="PK856_10895"
     CDS             complement(<1..580)
                     /gene="modA"
                     /locus_tag="PK856_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003793555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRL02914.1"
                     /translation="MKKPLLLSMLLMGITFPSVAADITVAAAASLTDAFKVIAADFEK
                     KNPQTKVKLTFASSGTLLQQLRNGAPIDVLATADQQTMNDAQAQSLIIKASRKDFAAN
                     QLVLVTPANSRLVINALAGLNQAGVKRIAMGNPAHVPAGRYAQAALEQSKQWQGLQAK
                     LIKAQNVRQALDYVARGETEAGFVFATDAISEK"
     gene            complement(589..1161)
                     /gene="mog"
                     /locus_tag="PK856_10900"
     CDS             complement(589..1161)
                     /gene="mog"
                     /locus_tag="PK856_10900"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HRL02915.1"
                     /translation="METVKIGLVSVSDRAYNGVYEDQGLPALKEWLSLALKNPFEVIE
                     KLIPDEQDLIEATLIELVDENHCDMVLTTGGTGPYPRDVTPDATLNVADRIMPGFGEQ
                     MRQVSLHFVPTAILSRQVGVIRKNSLILNLPGRPKAIAETLGGVKDAQGKTVVHGIFA
                     AVPLCVDAIGGPYIETHNNISETFRPKNFA"
     gene            complement(1170..>2059)
                     /locus_tag="PK856_10905"
     CDS             complement(1170..>2059)
                     /locus_tag="PK856_10905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HRL02916.1"
                     /translation="VIAQEDVTVSEGCLIAKASPQPGQHIRRQAEEYAMGEVLLPKQM
                     RLNAAAVGVIASQGLHQVSCFRRLKITVLSSGNELQVPGGELQENQIFDSNRAMLLAA
                     LADPCFEVCDGGLLADELSTITDNLQGAAVDSDVVLVSGGASVGDKDYVKEALSDLGN
                     IQHWKLAIKPGKPFGWGQIGHTQVFLLPGNPVASWVTFLALVQPALRVLAGCSQDMAR
                     PQTVSAKAEFDVLKAQSRLQFLRGNVDVFTGRLSAHIHPQQGSAMLGVCAHSNALIMI
                     PADTTISRGDGVDVMYLSA"
BASE COUNT          512 a          567 c          462 g          518 t
ORIGIN      
        1 ctttttcact gatggcatcg gttgcaaaca caaaacccgc ctcggtttca ccacgcgcca
       61 cgtagtccaa agcctgacgc acgttttgtg ccttgattaa cttagcttgc aaaccttgcc
      121 actgtttgct ttgttccaaa gcggcttggg catagcgtcc agcaggcacg tgagcagggt
      181 tgcccatggc gatgcgtttc acaccagctt ggtttaaacc agcaagagcg ttgattacta
      241 agcgactgtt cgcaggtgtc accaacacca attgattggc tgcaaaatcc ttacgactgg
      301 ctttaatgat taaagactgt gcttgtgcat cgttcattgt ttgttggtcg gcggtggcca
      361 atacatcaat gggggcacca ttgcgcaatt gctgcaacag ggttccagag gaggcaaaag
      421 tcaatttaac cttggtttga gggtttttct tttcaaagtc ggcagcgatg actttaaagg
      481 catcggtcaa actggccgct gcggccacgg taatgtcggc tgccacactg gggaaagtga
      541 tgcccatcaa tagcatgctg agcaataatg gttttttcat aaggagcctc aagcaaaatt
      601 tttgggtctg aatgtttcgg aaatgttgtt gtgtgtttcg atgtaaggcc caccgatggc
      661 atccacacac aaaggcaccg cagcaaaaat accgtgtacc actgttttac cctgtgcgtc
      721 ttttacccca cccaaggttt ctgcgatggc tttagggcgg cctggcaaat tcaaaatcag
      781 gctgttcttg cgtatcacac ccacttggcg tgacaaaatc gcagtgggca caaaatgcaa
      841 actcacttgc cgcatttgct ctccaaaacc tggcatgatg cgatcggcca cattcaaggt
      901 ggcgtctggc gtgacatcgc gcggataagg gcccgtgcca cccgttgtga gcaccatgtc
      961 acaatggttc tcatctacca attcaatgag tgttgcttca atcaaatcct gttcatcggg
     1021 tatcagtttt tcaatcactt caaaagggtt ttttaaggcc aaacttagcc attctttcaa
     1081 tgcaggtaag ccttggtctt catacacgcc gttgtaagcc ctgtcgctta ccgataccaa
     1141 tccaattttt acggtttcca tacccattcc taagctgata aatacatcac atcgacacca
     1201 tctccacggc ttatcgtggt atcggcaggt atcatgatta aggcattgct gtgggcacac
     1261 acacccagca tggcactgcc ttgctgcggg tgtatgtgtg cagacaagcg gcctgtaaac
     1321 acgtctacgt tgccacgcaa gaattgcaga cggctttgcg ccttcagcac atcaaattcc
     1381 gccttggccg atacggtttg aggccgtgcc atgtcttgag aacacccagc caaaacacgc
     1441 aatgcaggct gtaccaaagc aagaaaggta acccaactgg ccacaggatt gcctggcaac
     1501 aaaaacacct gtgtgtggcc tatctgtccc cacccaaatg gtttaccagg cttaatcgcc
     1561 agtttccaat gctgaatatt gcccaaatca gacagagcct ctttgacata atccttatcc
     1621 cccaccgagg ccccacccga taccaacacc acatcactgt ctacagcagc gccttgcaaa
     1681 ttgtcagtga tggtgctcaa ttcatcggca agcaagcccc catcacacac ctcaaaacac
     1741 ggatcagcca gtgccgccaa taacatggcg cgattgctgt caaaaatttg attctcttgc
     1801 aactcaccac caggcacttg caactcattg ccactggata acacggtgat tttcagtcga
     1861 cgaaagcatg acacttggtg caaaccttga ctggctatca ctcccaccgc cgccgcattc
     1921 aaacgcatct gtttgggaag caacacctcg cccattgcat attcttcagc ttggcggcgt
     1981 atgtgttgac caggttgcgg gctagcctta gcaatcaaac acccttcgct cacggtcaca
     2041 tcttcttgag cgatgaccg
//
LOCUS       DAOMVK010000410         2054 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_16066_length_2054_cov_4.132066, whole genome shotgun sequence.
ACCESSION   DAOMVK010000410 DAOMVK010000000
VERSION     DAOMVK010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16066_length_2054_cov_4.132066"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="PK856_10910"
     CDS             complement(<1..1017)
                     /locus_tag="PK856_10910"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003798845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HRL02917.1"
                     /translation="MAKLRNYTINFGPQHPSAHGVLRLILELEGETIVRADPHIGLLH
                     RGTEKLAETRTFIQNLPYMDRLDYVSMMCNEQAYCLAIEKMAGINVPIRAQYIRVMFA
                     EITRILNHLMNIGAQGLDIGAMTVILYCFREREDLMDIYEAVSGARLHAAYFRPGGVY
                     RDLPDFMPKYESNKFHNAKQLKQMNQAREGTMLDFIEDFTNRFPGCVDEYENLLTDNR
                     IWKQRLVDIGIVTPERALNKGFTGVMLRGSGIEWDVRKKQPYDAYANVDFDIPVGVNG
                     DCYDRYLCRIQEMRESNKIIKQCIKWLRENPGPVITDDHKFAPPRRVDMKENMEELIH
                     HFKFF"
     gene            complement(1010..1600)
                     /locus_tag="PK856_10915"
     CDS             complement(1010..1600)
                     /locus_tag="PK856_10915"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRL02918.1"
                     /translation="MTKIETLQAALQEALGAKISHMKADLGRVTIECRAADYLEVMKT
                     LRDHPKLQFEQLIDLCGMDYSEYREQVWEGFRFAAVSQMLSVSLNHRVCIKVFAEDDE
                     MPLLDSVTGIYSSADWFEREAFDLYGILFNGHPDLRRILTDYGFVGHPFRKDFPVSGY
                     VEVIYDQEQERVVYQPVTIEPREVTPRIVREEQYGG"
     gene            complement(1612..>2054)
                     /locus_tag="PK856_10920"
     CDS             complement(1612..>2054)
                     /locus_tag="PK856_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HRL02919.1"
                     /translation="TSADTVLNYMRTGSMWPVTFGLACCAVEMMHAGMARYDLDRFGI
                     IFRPSPRQSDLMIVAGTLCNKMAPALRKVYDQMSEPRWVVSMGSCANGGGYYHYSYSV
                     VRGCDRVVPVDVYVPGCPPTSEALIYGLLQLQNKIKRTSTIARN"
BASE COUNT          603 a          516 c          414 g          521 t
ORIGIN      
        1 aaagaattta aagtggtgaa tcaactcttc catgttttct ttcatgtcaa cacggcgagg
       61 aggggcaaat ttgtgatcgt ctgtaatcac agggcctgga ttttcgcgca accatttgat
      121 gcactgcttg atgattttat ttgattcgcg catttcttga atacggcaca aataacgatc
      181 gtaacaatca ccgttcaccc ctactggaat gtcaaaatcc acattggcat aggcgtcata
      241 aggttgtttt ttacgcacat cccactcaat accagaaccg cgcaacatca cacccgtaaa
      301 gcctttattt aaggcacgct caggtgtcac aatgccaatg tctaccaaac gctgcttcca
      361 aatgcggtta tcggtgagca agttttcata ctcatccacg caacctggga agcgattggt
      421 aaagtcttca atgaaatcaa gcatagtgcc ttcacgcgct tgattcattt gtttcaattg
      481 tttggcattg tggaatttgt tgctttcata cttaggcatg aaatctggca aatcacggta
      541 tacgccacca ggacggaagt aagcggcatg taaacgggca ccagaaacag cctcgtaaat
      601 gtccatcaaa tcttcacgct ctcggaagca atacaaaatc accgtcatgg caccgatatc
      661 caaaccttgg gcaccaatgt tcattaaatg attcaaaatg cgggtaattt ccgcgaacat
      721 cacacgaatg tattgcgcac gaatgggtac gttgatgcca gccatttttt caatggccaa
      781 gcaatatgcc tgctcattac acatcataga cacatagtct aagcggtcca tgtaaggcaa
      841 attttgaatg aaagtgcggg tttccgctaa tttttctgta ccacggtgca acaaaccaat
      901 gtgcggatcc gcacgaacaa tggtttcacc ctcaagctcc aaaatcaaac gcaacacacc
      961 atgcgctgat gggtgttgtg ggccaaagtt aatggtgtaa tttctgagtt tagccaccgt
     1021 actgctcctc tcgcacgatg cgtggtgtga cttcacgcgg ctcaatggtc acaggctggt
     1081 aaaccacacg ctcttgctct tgatcgtaaa ttacttctac ataacctgaa actgggaagt
     1141 ctttacggaa aggatgtccg acaaaaccat aatccgtcaa aatgcgacgc aaatcaggat
     1201 ggccattgaa taaaatgcca tacaagtcaa acgcttcgcg ctcaaaccaa tcagcgctgc
     1261 tgtatatgcc tgtgacagaa tccaaaagtg gcatttcatc gtcttcagca aacactttga
     1321 tgcacacacg gtgattcaaa gacactgaca acatttgtga aacggcagca aaacgaaaac
     1381 cttcccacac ttgttcacgg tactcgctgt aatccatgcc acacaaatct atcaattgct
     1441 caaattgcaa ttttggatgg tcacgcaagg ttttcattac ttccaaataa tcagcggcgc
     1501 ggcattctat ggtcacacga cctaagtctg ctttcatgtg gctgattttg gcacccaaag
     1561 cttcttgcaa tgcggcctgt aaagtttcta tcttagtcat ttacccgcct cttaattacg
     1621 cgcaatcgtt gaggtacgtt tgatcttgtt ttgcaactgt aacaaaccgt aaatcaacgc
     1681 ttccgaagtt ggagggcaac ctggcacata cacatccacc ggcacaacac ggtcacaacc
     1741 gcgcaccact gaatatgaat agtggtaata accaccgcca ttggcacaag aacccataga
     1801 aaccacccaa cgtggttctg acatttggtc atacactttg cgcagtgcag gagccatctt
     1861 gttacacaaa gtacccgcaa caatcatcaa atcagattgg cgaggacttg gacggaaaat
     1921 gataccaaaa cggtccaagt cataacgtgc cataccggca tgcatcattt ctactgcgca
     1981 acaagccaaa ccaaacgtta caggccacat agagccagta cgcatgtaat ttaaaacggt
     2041 atcagcactc gtgg
//
LOCUS       DAOMVK010000411         2052 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_16090_length_2052_cov_2.982974, whole genome shotgun sequence.
ACCESSION   DAOMVK010000411 DAOMVK010000000
VERSION     DAOMVK010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16090_length_2052_cov_2.982974"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..1046
                     /gene="alr"
                     /locus_tag="PK856_10925"
     CDS             9..1046
                     /gene="alr"
                     /locus_tag="PK856_10925"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRL02920.1"
                     /translation="MNLANLRHNFQYLKSKHGNKTLAVIKANAYGHGSVPCANALADL
                     ADGFAVACIEEALTLREAGVTLPIVLLEGVFEADEYALIEQHDLWPVVQNEVQLQALL
                     ATDIKKPLTVWLKMDSGMHRAGFFPQDYAAAFKRLQAAPQVAKIVKMTHFARADEPSV
                     GMTEMQIAAFDAGCAGCEGEESMSNSAGILDVPAAHRDWGRAGLALYGLSPMPEADAN
                     LKPVMTFKTKVFGVRELAAGEVIGYGSIFKTEKPTRVGLIAVGYADGYPRLASNDSPL
                     LIDGKLSRMIGRVSMDMSMVELNDDSQGIGSEVELWGERISANAVAAVAGTISYELLC
                     NVKRARFVYQD"
     gene            1229..1489
                     /locus_tag="PK856_10930"
     CDS             1229..1489
                     /locus_tag="PK856_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HRL02921.1"
                     /translation="MGMIGTIIVGFIVGLLARALHPGDDNIGWIWTIILGILGSFVGG
                     WVGQAAGWYQPGQPAGWIVSTLVAIVLLFLYYKVRGIKMGKK"
     gene            complement(1603..1758)
                     /gene="rpmG"
                     /locus_tag="PK856_10935"
     CDS             complement(1603..1758)
                     /gene="rpmG"
                     /locus_tag="PK856_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRL02922.1"
                     /translation="MRDKIKLESSAGTGHFYTTTKNKRNMPGKMEIKKFDPVVRKHVI
                     YKETKLK"
     gene            complement(1774..2007)
                     /gene="rpmB"
                     /locus_tag="PK856_10940"
     CDS             complement(1774..2007)
                     /gene="rpmB"
                     /locus_tag="PK856_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRL02923.1"
                     /translation="MARVCVVTGKRPMTGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRIRVSNAALRTIDKLGIDAVLADLRARGEA"
BASE COUNT          493 a          388 c          567 g          604 t
ORIGIN      
        1 aagcacacat caatttggcc aatttgcgcc acaattttca atatttaaaa agcaagcacg
       61 gcaacaaaac tttggcagtg attaaggcca atgcctatgg tcatggctcg gtgccgtgtg
      121 caaatgcgtt ggccgatttg gccgatggtt ttgccgtggc ctgtattgaa gaggctttga
      181 ctttgcgcga ggcgggtgtg acattaccca tcgtgttgtt agaaggcgtg tttgaggctg
      241 atgagtatgc attgattgag caacacgatt tgtggcctgt ggtgcaaaat gaagtgcaat
      301 tacaggcttt gttggcgaca gacatcaaaa aaccactgac agtatggttg aaaatggaca
      361 gcggcatgca ccgtgcgggt ttcttcccgc aagactatgc ggctgccttt aagcgtttac
      421 aggccgctcc tcaggtagca aaaatcgtga aaatgactca ctttgctcgt gcggatgagc
      481 catctgtggg catgacggaa atgcaaattg cggcgtttga tgcaggttgt gcagggtgtg
      541 agggcgaaga gtccatgtcg aattctgcgg gcattttgga tgttcctgcg gcgcaccgtg
      601 attggggtcg tgcgggtttg gcgctgtatg gtttgtcacc catgcctgaa gccgatgcca
      661 atttgaagcc tgtgatgacc tttaaaacca aagtgtttgg cgtgcgtgaa ttggcagcag
      721 gcgaagtgat tggttatggc tctattttta aaaccgaaaa gccaacccgt gtgggcttaa
      781 ttgccgtggg ttatgccgat ggttatccgc gtttggcttc aaacgacagc ccgttgttga
      841 tagatggtaa attgtcacga atgataggcc gcgtttctat ggacatgtcc atggtggagt
      901 tgaatgatga cagtcaaggc ataggcagcg aggttgagct ttggggtgag cgtatcagtg
      961 ccaatgcggt ggcggcagtg gcaggcacca tcagctatga attgctctgc aatgtgaaac
     1021 gcgcacgttt tgtttaccaa gattgataat gtgtcgtaaa agcggcctgt aagcatttac
     1081 aggccgcttt ttcattgcct tgtttaagtc ttgtctttta tgcttggtat attgaattgg
     1141 cattgaaatg ttgtatttgg gaactaatgg agggtatgtg actctgttgg ctcatgttgg
     1201 aattgaattg aattgaattg atgaggagat tggtatgata ggaaccataa tcgtgggctt
     1261 tattgttggc ttgttggcgc gcgcattgca cccaggtgat gataatatag gctggatttg
     1321 gacgataatc ttgggcatct tgggctcatt tgtcggtggc tgggtcgggc aggcagcggg
     1381 ttggtatcaa cctggtcaac cagcgggttg gatcgtgtct actttggtgg ccatcgtgtt
     1441 gctgtttttg tattacaaag ttcgtggcat caaaatgggc aagaaataag ctgatatatt
     1501 gatgaaagcg gcctgtaaac atttcatggt ttacaggccg cttatttgag gcaataaaaa
     1561 agccttctgt gaagaaggca attttatgag aagcaaatcg aattatttca atttggtttc
     1621 tttgtagata acgtgcttgc gaacaacggg atcaaatttt ttgatttcca ttttgccagg
     1681 catgttacgt ttgtttttgg tagtggtgta gaagtgaccc gtaccagcag atgattccag
     1741 tttgatttta tcgcgcattt cgcttattcc ttattacgct tcgccgcgtg cacgcaaatc
     1801 agccaataca gcgtcaatgc ctaatttatc aatagtgcgc aaagcagcgt tagaaacgcg
     1861 gatgcgtacc caacggtttt cgctttcaac ccaaaaacga cgtgattgca agttaggcaa
     1921 aaagcggcgt ttggttttgt tgttggcatg tgacacgttg ttgcccgtca tagggcgttt
     1981 accggtcact acacatactc gtgccatggt tagactccaa attaccgatt agtaaaaacg
     2041 agatttatat ca
//
LOCUS       DAOMVK010000412         2043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_16176_length_2043_cov_2.759557, whole genome shotgun sequence.
ACCESSION   DAOMVK010000412 DAOMVK010000000
VERSION     DAOMVK010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16176_length_2043_cov_2.759557"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="PK856_10945"
     CDS             complement(<1..415)
                     /locus_tag="PK856_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRL02924.1"
                     /translation="MSETVTIAAIATAPGRGGVGVIRLSGQNLLPLAATITGGKTPKP
                     RLALYSDFLAADGNPIDNGLLLYFAAPASFTGEDVIELQGHGGPVVMQMLLSRCLELG
                     ARMAEPGEFTKRAFLNNKLDLAQAESVADLIDASSQ"
     gene            complement(518..>1452)
                     /locus_tag="PK856_10950"
     CDS             complement(518..>1452)
                     /locus_tag="PK856_10950"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02925.1"
                     /translation="SAFAQNAFMLSCARVIIGVASGLISTSGMMGMLQTSPLPNEAQT
                     ARWYASLNGLGFGMGPLVGGLVAQISGGSVLLPYLPVIVLTAVVLYQLWHIPFHTPSA
                     PKPVDLKPRLALPEIGLGRKQFYLAAAGAFAAFAMFSLYASLAGSFIAEFLPWHGPLV
                     SGSAIAMVLFLSSATQFFLRRLSLAKTYAAATAMILVANVMLVLTLELHSLPLFLLSI
                     LATAMAHGLTLMAGFGKVQEITHSHNRAAVVATFLVAGYCGAILPVIALGWLADHLGI
                     STAVILYCTTFALWAIALFTGFVRLKRQREAQTI"
     assembly_gap    1453..1476
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1477..1726)
                     /locus_tag="PK856_10955"
     CDS             complement(<1477..1726)
                     /locus_tag="PK856_10955"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02926.1"
                     /translation="MQKRWIPFYWVCAALISGTMGTALASPLYPLYQQLWQLQPSDIT
                     TMFIAYMGGTLLSILFLGRLSDSLGFAKVLRAGLLLVLL"
     gene            1725..1847
                     /locus_tag="PK856_10960"
     CDS             1725..1847
                     /locus_tag="PK856_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02927.1"
                     /translation="MMKWVTYKMNEEKQRCGLQAAFDANPTCAVRQFCVAITVM"
     gene            complement(1861..>2043)
                     /locus_tag="PK856_10965"
     CDS             complement(1861..>2043)
                     /locus_tag="PK856_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02928.1"
                     /translation="KVAPANKKFPHLHDFLFASRPAITVHNVATSPHVLLELTQTIQV
                     KTCQMTRHLTQDKLCL"
BASE COUNT          522 a          499 c          603 g          395 t
ORIGIN      
        1 attggctgga ggcatcaatc aaatcggcca cgctttcggc ttgggccaaa tcgagcttgt
       61 tgttcaaaaa agcgcgcttg gtgaattcgc ctggctcggc catgcgcgcg cccaattcca
      121 agcagcggct gagcagcatt tgcatcacca cagggccacc gtggccttgc aattcaatca
      181 cgtcttcgcc ggtgaagctg gcgggcgcgg caaaatacag caacaaacca ttgtcgatgg
      241 ggttgccatc ggcggccaaa aaatcgctgt acaaagccaa gcgcggtttt ggggttttac
      301 cgccggtgat ggtggcagcc agtggcaaca ggttttgccc cgacaaacga atcacaccca
      361 cgcctcctcg accaggagcg gtggcaatgg cggctatggt gacggtttct gacatcgaac
      421 ttactcaact ctataaataa ccccgccatt gtaacgcaac aggcggcctg taagtttaca
      481 ggccgcctgt ttaagtggca gagcaaggct catggcatca aatggtttgt gcttctcgct
      541 ggcgttttaa gcgcacaaaa cctgtgaaca aggcgatggc ccacaaggca aacgtggtgc
      601 agtaaaggat gacggcagtg ctgatgccca agtggtcggc caaccagccc aaggcgatga
      661 caggcaagat ggcgccacaa tagcctgcca ccaaaaaagt ggccaccact gcggcacggt
      721 tgtggctgtg ggtgatttct tgcactttgc caaagccggc catcagggtt aagccgtgtg
      781 ccatggcggt ggcgaggatg ctgagtaaga acaagggcaa ggagtgcagc tctagggtca
      841 acaccagcat gacattggcg acaagtatca tggcggtggc ggcggcatag gttttggcca
      901 agctcaaacg gcgcaagaaa aactgggtgg ccgaagacaa aaacaacacc atggcgatgg
      961 cgctgccgct gaccaaaggc ccatgccaag gcagaaattc ggcgatgaaa gagcctgcca
     1021 aagaggcata gaggctgaac atggcaaagg ctgcaaacgc gcctgcggcg gccagataaa
     1081 attgtttgcg gcccaagccg atttcaggca aggccaagcg cggttttaaa tccacaggct
     1141 tgggcgcgct gggcgtgtga aacgggatgt gccacagttg gtataacacc accgcggtca
     1201 aaacgatgac gggcaaataa ggcagcaaca ccgagccgcc gctgatttgt gcgaccaagc
     1261 cgccgaccaa aggccccatg ccaaaaccca agccattcaa agaggcgtac caacgcgcgg
     1321 tttgggcttc gttgggcaag gggctggttt gcaacatgcc catcatgccc gaggtgctga
     1381 tgaggcctga ggcaacgccg atgatgacgc gggcgcaaga gagcatgaag gcgttttgcg
     1441 cgaaggcaga cannnnnnnn nnnnnnnnnn nnnnnnccag caagaccagc agcaaacctg
     1501 cgcgcaagac tttggcaaag cccaagctgt cgctcaagcg ccccaaaaac aaaatcgaca
     1561 acaaggtgcc gcccatgtag gcgatgaaca tggtggtgat gtcactgggt tggagttgcc
     1621 acagctgttg gtaaaggggg taaagcgggc tggccaatgc cgtgcccatg gtgccgctga
     1681 ttaaggcggc gcatacccag taaaaaggaa tccagcgttt ttgcatgatg aaatgggtga
     1741 cttataaaat gaatgaggaa aaacagcgct gtggtttaca ggccgcattt gatgctaatc
     1801 ctacttgtgc ggtgcgtcaa ttttgtgtgg cgataacagt gatgtgagtt gagggtgttg
     1861 tcacaggcaa agcttgtctt gggtcaaatg tcgagtcatt tggcatgttt tgacctgaat
     1921 tgtttgcgtt aactcaagaa gcacatgggg tgaagtcgct acattgtgaa ctgtgatagc
     1981 aggacggctc gcaaataaaa aatcatgcag gtggggaaat tttttattgg caggtgccac
     2041 ttt
//
LOCUS       DAOMVK010000413         2031 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_16282_length_2031_cov_3.350202, whole genome shotgun sequence.
ACCESSION   DAOMVK010000413 DAOMVK010000000
VERSION     DAOMVK010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16282_length_2031_cov_3.350202"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="PK856_10970"
     CDS             <1..644
                     /locus_tag="PK856_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRL02929.1"
                     /translation="IVFLVRKGNPKNIKDWGDLVKGDVQVITPNPKTSGGARWNFLAA
                     WAWAKQNNGGDEAKAKAFVTELFKRVPVMDTGARGSTITFTQRGLGDVLLAWENEAYL
                     ALKEAGDQYEIVTPSLSILCEPPVAVVDSVVDKKGTREVATEYLKGLYTEDAQRIIAK
                     NFYRPINPKVAEEFKDKFPQLQLVTIDKDFRGWKTAQQQFFADGGVFDQIYVK"
     gene            complement(715..1401)
                     /locus_tag="PK856_10975"
     CDS             complement(715..1401)
                     /locus_tag="PK856_10975"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HRL02930.1"
                     /translation="MLGLFLVFVLVAVAYVRLKLSGASLSALALKALTTVLCIVMAVY
                     AAHEQTHVSAWAAVVVLALVCGLMGDVFLDLKLIYRQDDAWFTFAGFIAFGVGHLLYW
                     AYFFTQFDWGSDAFLWWLGLNVVFVVGVWLTEKPMQLDYGRFRAISLLYAFVLLGVLL
                     QAAYAWWQQPSWQWAVFTVGMLAFVASDLILSQSYFSKRPEKPWMIIGNYVCYFGAQC
                     LVAWSVLASV"
     gene            complement(1498..>2031)
                     /locus_tag="PK856_10980"
     CDS             complement(1498..>2031)
                     /locus_tag="PK856_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRL02931.1"
                     /translation="IVVTNAGGPPPGDFRDWDKQAWFKAVEANMYTPIALIQATIDGM
                     AARGFGRIINITSSAVKAPIDVLGLSNGARSGLTGFVAGVARSGIAAQGVTINNILPG
                     LFDTDRLRGMNANAMQKTGKSQEQVVSERQKSIPVRRFGQPHEFGALCAFLSSDLAGY
                     ITGQNMLIDGGAYSGTF"
BASE COUNT          600 a          581 c          450 g          400 t
ORIGIN      
        1 ccatcgtgtt tttggtgcgc aaaggcaatc cgaaaaacat caaagactgg ggcgatttgg
       61 tcaaaggcga tgtacaagtg attacaccca atcccaaaac ctcaggcggc gctcgctgga
      121 attttcttgc tgcatgggcg tgggcaaaac aaaacaatgg cggcgatgag gccaaagcca
      181 aagccttcgt gaccgaattg ttcaaacgcg taccagtgat ggacacaggc gcacgcggct
      241 ccaccatcac ctttacccaa cgcggcttgg gcgacgtgtt gctcgcatgg gaaaacgaag
      301 cctatttggc tttgaaagaa gcaggcgacc aatacgaaat cgtcacccct agcctgtcta
      361 ttttgtgcga accacccgtg gctgtggtgg actcagtggt cgataaaaaa ggcacgcggg
      421 aagtggccac cgaatactta aaaggcctct acaccgaaga cgcacaacgc atcatcgcca
      481 aaaacttcta ccgccccatc aatccgaaag tggctgagga attcaaagac aaattcccac
      541 aattgcaatt ggtgaccata gacaaagatt tccgcggctg gaaaacagca cagcaacagt
      601 tcttcgccga tggcggcgtg tttgaccaaa tttacgtaaa gtaatctcag cttgtagcaa
      661 cacaaaaaag cggcctgtaa acatttacat gccgctttta ttaccaatag accatcatac
      721 cgatgccaat accgaccacg ccaccaagca ttgtgcacca aaataacaca cgtaattgcc
      781 gatgatcatc catggctttt caggacgttt actgaagtaa gattggctta aaatcaaatc
      841 tgatgcgaca aacgccaaca tgcccacggt aaacaccgcc cattgccacg aaggctgttg
      901 ccaccacgca taagcggcct gtaacaacac gcccaaaagc acaaatgcat acaacaagct
      961 gatggcccga aagcgaccat aatccagttg catgggtttc tcagtgagcc acacgcctac
     1021 cacgaagacc acattcaagc ccagccacca caagaaagca tcactgcccc aatcaaactg
     1081 agtgaagaaa taagcccaat acagcaaatg ccccacgcca aaagcgatga aaccagcgaa
     1141 ggtaaaccaa gcatcgtctt ggcggtaaat cagtttcaaa tccaaaaaca catcgcccat
     1201 caagccgcac accaaagcca acaccaccac ggctgcccat gcagaaacgt gggtttgctc
     1261 atgcgccgca tacaccgcca tgacgatgca caatactgtg gtgagcgctt ttaaagccaa
     1321 agcagataag ctcgcaccgc taagcttcag acgcacataa gccacagcca ccaacacgaa
     1381 tacaagaaac aaacccagca tagacagccc tttcaataca gaacaccatt gtagccacaa
     1441 acaaatacgg cctgtaaacc gtttacaggc cgcatatttg atgttttcaa ccagtagtta
     1501 gaatgtgcct gaatacgcac cgccatcgat gagcatgttc tgccctgtga tgtagcctgc
     1561 taaatcgctg ctcaaaaacg cgcacaaagc gccaaactca tgtggctgac cgaagcggcg
     1621 cacagggatg gatttttggc gttcgctcac aacttgttct tgtgatttac ctgttttttg
     1681 catggcattg gcattcatac cacgcaaacg gtcggtgtcg aacagacctg gcaaaatatt
     1741 gttgatggtc acgccttgcg cggcaatgcc actgcgagcc acgcccgcga caaagcccgt
     1801 caaaccactg cgcgcgccat tggacaagcc caatacatcg atgggcgctt ttaccgcact
     1861 ggaagtgatg ttgatgatgc ggccaaagcc acgcgccgcc atgccgtcta tcgttgcctg
     1921 tatcaaggca atgggcgtgt acatgttcgc ttccaccgct ttaaaccacg cttgtttgtc
     1981 ccaatcgcga aaatcacctg gtggcgggcc gcccgcattg gtcaccacaa t
//
LOCUS       DAOMVK010000414         2022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_16392_length_2022_cov_3.264362, whole genome shotgun sequence.
ACCESSION   DAOMVK010000414 DAOMVK010000000
VERSION     DAOMVK010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16392_length_2022_cov_3.264362"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            249..1832
                     /locus_tag="PK856_10985"
     CDS             249..1832
                     /locus_tag="PK856_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HRL02932.1"
                     /translation="MALTSGYKKVGKWLGRLLLILCIGIGVTMIGCRLEPPQGRTQSV
                     HLSAAETQLTTLGQVVNQEQAKHPDLSGLYLLADPHEAFAARVKLAKQAEKTLDVQYY
                     IWRDDTTGTLLMEALHEAAERGVRVRLLIDDHGTSGLDEALSAMDAHPNIEVRLFNPF
                     VFRPFKLFGFVTEFDRANRRMHNKSFTADNAATIVGGRNVGDEYFGATDGVLFADLDA
                     LAVGDVVPKVSQSFDDYWQSGSVDALSDVMPPINAEQQKKWSQHTSIVKRSPAANEYV
                     HAFANMGFVQQLIAGQLPLEWAPTRFIVDSPSKGLAKAKHQELLINHLEEVLGKPQQS
                     LDLVSPYFVPTKAGTDYFIRLQQQGVKVRILTNSLAATDVVAVHSGYARYRQRLVEAG
                     VELYELKPTSNNLEDKKYVGFKGSSGSSLHAKTFGTDQNHMFIGSFNFDPRSINLNTE
                     LGIVVENPHYRQKMSEVFSDLLPQRSWRVQYDPAQQQLLWTDATQTPPQVLTEEPMST
                     WWQRASVAVMSVLPIEGFL"
BASE COUNT          502 a          496 c          532 g          492 t
ORIGIN      
        1 ttacctcagc cgccaacaaa gaccacagcc aattgttctt attggacgtg gacacaggcg
       61 caacccctgg catgaaaatc ggttaagcct gcgcagtgat tgtaagcaat gaaacaagcg
      121 gcctgtatta ttttacaggc cgctttttgt tggtttaggc aaagattgca atgcttggtg
      181 gttggctgcg cattattcgg tacaatttgg gcggaagcag tatggaacat gactgaggat
      241 gatgcggtat ggcgttgaca tcaggataca aaaaagtggg caaatggctg ggccgtttac
      301 tgctcatctt gtgcataggg ataggagtga caatgatagg ctgccgtttg gaaccaccac
      361 aaggacgcac gcaatcggtg catttatcgg cggctgaaac ccagctcacg actttgggtc
      421 aggtggtcaa ccaagaacag gccaaacacc ctgatttaag cggcctgtat ttgttggccg
      481 accctcatga agcctttgct gcacgggtga agctggccaa acaagccgag aaaaccttgg
      541 atgtgcagta ttacatatgg cgcgatgaca ccactggtac cttactgatg gaggctttac
      601 acgaggcggc agaacgcggt gtgcgcgtgc gcctgctgat tgatgaccac ggcaccagtg
      661 gcttagacga ggctttgtct gccatggacg cccatccgaa tatcgaagtg cgcttgttca
      721 acccgtttgt gttccgccca ttcaaactgt ttggctttgt caccgaattc gaccgcgcca
      781 atcggcgcat gcacaataag agttttaccg cggacaatgc cgccaccatc gtgggcgggc
      841 gcaatgtcgg cgatgagtat tttggtgcca ccgatggcgt gttgtttgcc gatttggatg
      901 ccttggctgt gggcgatgtg gtgccgaaag tgtcacagtc tttcgatgat tattggcaaa
      961 gtggctcggt cgacgcctta agcgatgtga tgccacccat caatgccgaa cagcaaaaaa
     1021 agtggtcaca acacacgtcc atcgtcaaac gctcccctgc ggccaatgaa tatgtgcatg
     1081 cgtttgccaa tatggggttt gtgcaacaat tgatagcagg gcaattgccg ctggaatggg
     1141 cgcctactcg cttcattgtc gattcgccca gcaaaggctt ggccaaggcc aaacaccaag
     1201 aattgctcat caaccatttg gaagaagtgc tgggcaaacc ccaacaatca ttggatttgg
     1261 tgtcgccgta ttttgtgccc accaaggcag gcacggatta cttcatccgt ttgcaacagc
     1321 aaggggtgaa agtgcgcatc ttaaccaatt ctttggcggc aaccgatgtg gtggcggtgc
     1381 attcaggtta tgcgcgctat cgccaacgct tggtggaagc aggcgtggag ttgtatgaat
     1441 tgaaacccac ttccaacaat ctcgaagaca aaaaatacgt gggttttaaa ggcagctcag
     1501 gttcaagttt gcatgccaaa acctttggta ccgaccaaaa ccacatgttc attggctcgt
     1561 tcaatttcga cccgcgctcc atcaatttga ataccgagtt gggcatcgtg gtggaaaacc
     1621 cgcattaccg tcaaaaaatg agcgaagtct tctccgattt gttgccgcag cgcagctggc
     1681 gcgtgcaata cgaccctgct cagcaacaac tgctgtggac agacgccacc caaacaccac
     1741 cacaagtgtt gaccgaagaa ccaatgtcta cgtggtggca acgtgccagt gtggcggtga
     1801 tgagtgtatt gccgatagaa ggtttcttgt aatgccgact taagcggcct gtaaacacgc
     1861 aggtctgttt tgtgcttatg tgacgctcct tcgcgttttc cccattaaaa agcggcctgt
     1921 aaacgattta caggccgctt gtgacactca gtatcaatga aaaaccatca atccgaacgt
     1981 tgcatcaaaa atttggcttt gaattcatcc catttttgtt tg
//
LOCUS       DAOMVK010000415         2008 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_16533_length_2008_cov_2.601126, whole genome shotgun sequence.
ACCESSION   DAOMVK010000415 DAOMVK010000000
VERSION     DAOMVK010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16533_length_2008_cov_2.601126"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PK856_10990"
     CDS             <1..305
                     /locus_tag="PK856_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02933.1"
                     /translation="QADVVSISRDQSSQVKPIYTEPCANGIEMNRYRVSASDGHANLR
                     KAPNTNAPIQQRLTNQTTLLELGKKGAWIEVQVIEQERNRAVGFVHQSQVTLMDGE"
     gene            377..637
                     /locus_tag="PK856_10995"
     CDS             377..637
                     /locus_tag="PK856_10995"
                     /inference="COORDINATES: protein motif:HMM:NF027249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRL02934.1"
                     /translation="MLFYLAVYKEDVQDYWAEVPALEGCYSVGNSVSETLNNSRALIK
                     IHVDDLRADCPHYTVVQKSLSALKALPQYKGAAWHAVKVDVS"
     gene            complement(741..1271)
                     /locus_tag="PK856_11000"
     CDS             complement(741..1271)
                     /locus_tag="PK856_11000"
                     /inference="COORDINATES: protein motif:HMM:NF025024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HRL02935.1"
                     /translation="MLALSEKVWHIPQFLQPEQCQQLLDLALQHGFVPAQVRAASGTR
                     AMPMVRNNLRAQLPSPEWVSVLWQGLLAHDLPDIAGRQACGLPRDLRFYQYQPEQRFK
                     MHKDGPWLEDGLRSELTLLVYLNEDFQGGATDFREFKVLPKTGDAVLFVHDTWHEGTV
                     LEAGCKYVLRSDVMYS"
     gene            complement(1304..1822)
                     /locus_tag="PK856_11005"
     CDS             complement(1304..1822)
                     /locus_tag="PK856_11005"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRL02936.1"
                     /translation="MDMKIGFAQAQETDALVDLINQAYRGSESWTGERDLISGARTNA
                     TEIAGLIADAQAQFLVLRDEAKQILGCIVAEKAGDYVYLGMFAINPSVQQTGLGKALL
                     AGAEHFARGFWRGIKGSKMTVITARTELLAYYERRGYVQSNELQAFPRDITVGEPKQE
                     LQLVTLYKPFAV"
BASE COUNT          525 a          558 c          461 g          464 t
ORIGIN      
        1 ttcaggccga tgttgtgagc atcagccgcg accaaagcag tcaagtcaaa ccgatttata
       61 ccgaaccttg tgccaatggc attgaaatga accgttaccg agtgagcgca agcgatggcc
      121 atgccaacct acgcaaagcc cccaacacca acgccccaat acaacagcgc ttaaccaacc
      181 aaactacctt actggagcta ggcaaaaaag gggcatggat agaggttcaa gtcatcgaac
      241 aagagcgcaa ccgagccgtt ggtttcgtgc accaaagcca agtcacttta atggatggtg
      301 agtgatgctg catgcgcctc aaccagcatt aaacgggtgt tgtgcatcag gccattgatg
      361 ggttaaggag gatgggatgc tgttttattt ggcagtttac aaagaagatg tgcaagatta
      421 ttgggccgaa gtacccgctc tagaaggatg ttactctgtt ggcaacagcg tctccgaaac
      481 actcaacaac agccgtgccc tcatcaaaat ccatgtggat gatttgcgcg cagactgccc
      541 ccattacact gtggtgcaaa agtctttgtc ggcactgaaa gccctgcctc aatacaaagg
      601 cgcagcttgg catgcggtca aagttgacgt gagttgaacc tagtcttaca tatcaaatgg
      661 ccgatgcatc aaacacaaat aagcggcctg taaaccctac aaataggttt acaggccgct
      721 tattcaaaca cacacatgtt ttaagaatac atcacatccg agcgcaacac gtatttgcac
      781 cctgcttcta aaaccgtgcc ttcgtgccaa gtatcgtgaa caaacaagac cgcatcgcct
      841 gttttcggca acactttaaa ctcgcgaaaa tcggtggcgc caccttgaaa atcctcattc
      901 aaatacacca acagcgttaa ctcgctgcgc aaaccgtctt ccagccacgg cccgtctttg
      961 tgcattttaa agcgctgctc aggttggtat tggtaaaagc gcaaatcgcg gggcaaaccg
     1021 caagcttgtc gccctgcgat gtcaggcaaa tcgtgcgcca acaagccttg ccataacacg
     1081 cttacccact caggcgatgg taactgcgcg cgcagattgt tgcgcaccat gggcatcgcc
     1141 cgtgtgcctg aagcagcccg aacttgtgca ggcacgaagc catgttgtaa ggccaaatcc
     1201 agcaattgct gacattgctc aggctgcaaa aattgtggga tgtgccacac cttctcactt
     1261 aaagccaaca tcgctgcacc ccttggtcac tgctcaccga gttttagacg gcaaacggtt
     1321 tgtacaaagt caccaattgc aattcttgtt tcggttcacc cactgtaata tcgcgtggaa
     1381 aggcttgcaa ttcattcgat tgcacatagc cacggcgctc gtaataggcc aacaattcag
     1441 tgcgcgcggt aatcaccgtc attttgctgc ctttgatgcc gcgccaaaaa ccacgagcga
     1501 aatgctccgc gcccgcaagc aaagccttgc ccaagcctgt ttgttgcacg cttggattga
     1561 tggcaaacat ccccaaatac acataatcgc ctgctttttc cgccacaata cagcccaaga
     1621 tttgctttgc ctcgtcgcgc aacaccaaaa actgcgcttg ggcatcagca atcaagcccg
     1681 caatttcagt cgcattggtg cgtgcaccac tgatcaaatc gcgctcaccc gtccacgatt
     1741 cgctgccgcg gtaggcttgg ttaatcaaat ccaccaaagc atcggtttct tgtgcttggg
     1801 caaagccaat tttcatgtcc atctgtgttt ccttgttggc caatcttaca ggccgcttat
     1861 cacaggcctc gtgcccattc ggggcgcaat cgtgcttgtt gtgggttgag tatggcttgt
     1921 tgaatttccg cgagcaagcg tggtttgtca cggcccaaac gcccttttaa caccaaataa
     1981 ttcgaagcat ggtcgctgcg aaacacgg
//
LOCUS       DAOMVK010000416         2007 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_16546_length_2007_cov_3.173156, whole genome shotgun sequence.
ACCESSION   DAOMVK010000416 DAOMVK010000000
VERSION     DAOMVK010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16546_length_2007_cov_3.173156"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(393..1556)
                     /locus_tag="PK856_11010"
     CDS             complement(393..1556)
                     /locus_tag="PK856_11010"
                     /inference="COORDINATES: protein motif:HMM:NF005704.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HRL02937.1"
                     /translation="MGIGVSIVGSGHTKFGRLQENLEQLIVAASQEALQEAGITGNEV
                     DAVFLGHFNAGMAADAFASSLILQADEGLRFKPATRFENACASGAAAVYAAIDAIQSG
                     RIEVALVVGVEKMAHNDTAGVTRALAGAGYQNHPDEAGVSFPAVFARAAQIYGERHGD
                     PMIAMAKIAAKNHSNAMLNPYAQLRKDLGFEFCNTVSEKNPLIEPSLRMSDCSLISDG
                     AAAVVLVAEHRAAEFALRAHFAAAVHVSDLLPLHSRDWASFIGPEKAIQAAYAQAGIS
                     VQDLNFAEVHDCFTIAELLLYEAMGLADKGKGFEALDSGTVYRDGALPVNVSGGLKAK
                     GHPVGATGVSMHVLAYRQLTGQAGEMQLAAPKLGLVFNMGGAAVANYASVLSA"
     gene            complement(1829..>2007)
                     /gene="grpE"
                     /locus_tag="PK856_11015"
     CDS             complement(1829..>2007)
                     /gene="grpE"
                     /locus_tag="PK856_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRL02938.1"
                     /translation="SEINPSVGDKLDPHLHQAMQAVESEQEPNTVVSVMKKGYSLNDR
                     VLRPAMVVVAKAAE"
BASE COUNT          488 a          566 c          448 g          505 t
ORIGIN      
        1 aaatcgccaa acaagagttg gttgtaccta agtcaatacc aattacttta gccatattct
       61 gtgtctccaa atagtgggtg tgtcacccaa taaagtgttt gttaattcat caatgattgc
      121 tatatggtgg ggcttggcga tatttcaagt gtattttgca caatattgat gcttgttgaa
      181 acacacgccc aaccctgctc tcgcagctaa aatgggctgc ctgtggcaaa attcaagggg
      241 aagtggccaa attctgtccg cataagattt aattctttgg tacaagctgt gtcattcaat
      301 gcgttctaac ctttaaacac ataagtggcc tgtaatgact tacaggccac ttatttcata
      361 cggatctaac ctctaagacc aacatgtctg ctttatgctg acaataccga agcgtaattg
      421 gcgactgccg cgcctcccat gttaaacacc aatcccaact tgggcgcggc caattgcatc
      481 tcgcctgctt gccctgttaa ttggcggtag gccaacacgt gcatggacac ccccgtcgcg
      541 ccgacaggat ggcctttggc ttttaagcca cccgacacat tcacaggcag cgcaccatcg
      601 cggtataccg ttccgctgtc taaggcttca aaacccttgc ctttgtctgc caagcccatg
      661 gcttcgtata acagcaattc ggcgatggtg aaacaatcgt gtacttcagc aaaattcaaa
      721 tcctgcaccg agatgcctgc ttgtgcataa gcggcctgta tcgctttttc agggccgata
      781 aaactggccc aatcacggct gtgtaaaggt aataaatcac tgacatgcac ggctgcagca
      841 aaatgcgcac gcagcgcaaa ttcagccgca cggtgttcag ccaccaacac caccgctgcc
      901 gcgccatcgc tgatgagcga acagtcgctc atgcgcaaac tcggttctat caatgggttt
      961 ttctcggata ctgtgttgca aaactcaaaa cccaaatcct tgcgcaattg tgcatatgga
     1021 ttgagcatgg cattgctgtg gtttttggcg gcaattttgg ccatagcaat catagggtcg
     1081 ccatggcgct caccataaat ctgcgccgcg cgcgcaaaca ccgcaggaaa cgacacgcct
     1141 gcttcatcgg gatggttttg ataacctgcg cctgccagcg cacgggtcac gcctgcggtg
     1201 tcgttgtggg ccattttctc tacgcccacc accaaggcca cttcaatgcg gcctgattga
     1261 atcgcatcga tggccgcata caccgccgcc gcgcccgaag cacaggcgtt ttcaaaacgc
     1321 gtggcaggtt tgaaacgcaa accctcatcg gcctgtaaaa tcagcgatga agcaaaagca
     1381 tctgctgcca tgcccgcatt gaaatgcccc aaaaacaccg catccacctc attgccagtg
     1441 atgcccgcct cttgcaacgc ctcttgcgat gcggccacga tgagttgttc caaattttct
     1501 tgcaaacgcc caaatttggt gtggcctgag cccacgattg ataccccgat tcccattcgc
     1561 ttctcctttg tcagcgctgc cagtcttggg cagccatcaa gttgttttat gcaccaccct
     1621 agcacagctc tatcagtgct gcctatgggg gtgcaaaaca cacgatggcc aaccatcact
     1681 cctctgttca tacaagcggc ttgtatactc atggtttgta gtatgggctt gtgtcacaag
     1741 actcttcaca caatataaaa gaaaccaata aaaaagcggc ctgtaactgt tacaggccgc
     1801 ttttagcgat gaatccacgc tatcaaattt attctgcggc tttggccacc accaccatcg
     1861 cagggcgcaa cacgcggtcg ttcaagctat agcctttttt catcaccgaa accacggtgt
     1921 taggctcttg ctcactttcc actgcttgca tcgcttggtg caaatgtggg tctaacttgt
     1981 cacccacgct cgggttgatt tcgctga
//
LOCUS       DAOMVK010000417         2006 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_16557_length_2006_cov_2.768836, whole genome shotgun sequence.
ACCESSION   DAOMVK010000417 DAOMVK010000000
VERSION     DAOMVK010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16557_length_2006_cov_2.768836"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PK856_11020"
     CDS             <1..217
                     /locus_tag="PK856_11020"
                     /EC_number="2.6.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957464.1"
                     /note="catalyzes the formation of pyruvate and
                     beta-alanine from L-alanine and 3-oxopropanoate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRL02939.1"
                     /translation="GVEFESSAEGLGKRAGLIQQKCYEAGVLMRLTGETLALSPPLII
                     NEAQIDQIFSTIGEAVKAVANESAVKA"
     gene            complement(530..1921)
                     /locus_tag="PK856_11025"
     CDS             complement(530..1921)
                     /locus_tag="PK856_11025"
                     /EC_number="1.2.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050269 - coniferyl-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006081 - cellular aldehyde metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HRL02940.1"
                     /translation="MNSSSKLQEPFNVIFNASRQELAVPWPTRKRRLEQLQALLKEHQ
                     DTLLAAIATDFGQRSATETTILELIPSNSAIKHALKHGEKWMRRRKAPTSWLFKPATA
                     YVQPQPKGVIGIVSPWNYPLFLIIGPLVDAFVAGNRAMVKISEHVPKFAETMASLVPQ
                     YFQADELTIVTGGVEVASAFSALPFDHLLFTGSTSVGKHVMHAAANNLTPVTLELGGK
                     SPVIIATDADLPKAVERIMYGKMINAGQTCIAPDYVLLQHSQLDEFLRLAQVWFNKHY
                     PDWDSNTDFTRIINSTQLERLQTWLNEANSKGAKVLPLAKQRADFLTPCLVHSAPDSC
                     QLMQQEIFGPILPIVTVDSVQAAIDYINVRPRPLACYVFAEQNELIEQCVRKIVAGGM
                     CINDVVLHVAQDALPFGGIGPSGMGSYHGKTGFETMSHMKGVFVQKPKNMVALMAPPY
                     GKRVKTLLKFLKH"
BASE COUNT          491 a          467 c          525 g          523 t
ORIGIN      
        1 gggtgtggaa tttgaaagca gtgccgaagg cttgggcaaa cgtgctggtt tgattcaaca
       61 aaaatgttac gaagcaggcg tgttgatgcg cttgacgggt gaaaccttgg ccttgtcgcc
      121 tccattgatc atcaatgaag cgcaaatcga ccaaatcttc agcaccatcg gtgaagcggt
      181 taaagccgtt gccaatgaaa gtgctgttaa agcttaagca tagacggttt gaattgaaaa
      241 catcttgtga tgaataatca caggatgttt ttttatggat ggcgtttgta agcgaattgg
      301 gaaggaataa gcggcctgta acttggttta caggctgctt attatggtgt gagattcgta
      361 agtttgcatg gaattgcggc ctattcagaa cgatgcttac taagcaactc cttgcagtat
      421 tttagggata cagggcaaag gccaactaag ctttacaggc cgcttgtata cactggcaat
      481 caattcaaat cacgaagcaa accttgcaac acatggtttt cagagcttgc taatgcttta
      541 aaaatttcaa cagggttttg acccgcttgc cataaggtgg cgccatcaat gccaccatgt
      601 ttttaggctt ttgtacaaac acacctttca tgtgcgacat ggtttcaaaa cccgttttgc
      661 catggtaaga gcccatgcct gaagggccta tgccgccaaa aggcaaagcg tcttgcgcca
      721 catgcaacac cacatcattg atgcacatgc caccagcgac aatttttctc acgcattgct
      781 cgattaactc gttttgctcg gcaaacacat aacaggccaa tgggcgcgga cgcacattga
      841 tgtaatcaat agcggcctgt acgctgtcca cagtgacgat gggcagtatt ggcccgaaga
      901 tttcctgttg catcaattgg catgagtcgg gggcagaatg caccaaacaa ggcgttaaga
      961 aatccgcccg ctgtttggcc aagggcaata ctttggcccc tttgctgttg gcctcgttga
     1021 gccaagtttg caagcgttcc aattgtgtgc tgttgatgat gcgggtgaaa tcggtattgc
     1081 tgtcccaatc gggatagtgt ttgttgaacc acacttgagc caagcgcaaa aactcatcca
     1141 attggctgtg ctgcaataac acataatcag gtgcgatgca ggtttggcct gcattgatca
     1201 ttttgccata catgatgcgt tctactgcct tgggcaagtc ggcatcggtg gcgatgatga
     1261 cgggtgattt gccgcccaat tccagagtca caggcgtgag gttgttggcg gcagcatgca
     1321 tcacgtgttt gcccacactt gttgagcctg tgaacagcaa gtggtcaaat ggcaaggctg
     1381 aaaaagccga tgccacttcc acgccacctg tgacgatggt caactcatcg gcctgaaagt
     1441 attgtggcac caagctggcc atggtttcgg caaacttggg cacgtgttcg gagattttca
     1501 ccattgcccg attgcctgcc acaaacgcat cgaccaaagg cccgataatc aaaaacaacg
     1561 ggtaattcca cggggacacg atgccgatga cgcctttggg ctgcggctgt acatacgccg
     1621 tggcaggttt aaacagccac gaagtgggcg ctttgcgacg gcgcatccat ttttcaccgt
     1681 gtttcaaggc atgtttgatg gcactgttgg aaggaatcag ttccaatatg gtcgtttctg
     1741 tggccgagcg ttgcccaaaa tccgtggcaa tcgccgccaa taaggtgtct tgatgttctt
     1801 ttagcaaggc ttgtaattgt tccaagcggc gtttgcgcgt cggccaaggt acggcaagct
     1861 cttggcgact ggcattgaaa atcacattga agggttcttg gagtttactg ctggaattca
     1921 tgccaccgta ccttgcgttt gtttgtgacc attaacttgg gtgatgcgcc catcgggcag
     1981 gctgatggca gtgagttctc gtcccc
//
LOCUS       DAOMVK010000418         2005 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_16567_length_2005_cov_2.973333, whole genome shotgun sequence.
ACCESSION   DAOMVK010000418 DAOMVK010000000
VERSION     DAOMVK010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16567_length_2005_cov_2.973333"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="PK856_11030"
     CDS             complement(<1..327)
                     /locus_tag="PK856_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HRL02941.1"
                     /translation="MKEFDFIKQYLAARQDNDAQVVLGIGDDAAIIRPQADCDIHISS
                     DMLLGGRHFFTDVQPADLAHKVLAVNLSDMAAMGATPKWATLSVALPQLDEAWVRAFC
                     DSLFALA"
     gene            518..1378
                     /locus_tag="PK856_11035"
     CDS             518..1378
                     /locus_tag="PK856_11035"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRL02942.1"
                     /translation="MSDLDLQKIYSGKVRDLYAIDDKRMLMVATDRLSAFDVILDEPI
                     PTKGEILTQISNFWFDYLKDVMPNQFIGQTVYDVLPEAQAREYEKRAVVVKKLTPIKI
                     EAIVRGYLAGSGWKDYQNTGKLCGIDLPAGLQQAQQLPEVVFTPSTKAAVGEHDENIS
                     FDEAAKIIGEDLAHQVQTKAIELYKKAAAYAESRGIIICDTKFEFGTDENGTLILMDE
                     VLTPDSSRFWPADQYEVGTNPPSFDKQFVRDWLEASGWNKQAPAPKVPADVIAKTVAK
                     YEEALQLLTK"
     gene            1444..>2005
                     /locus_tag="PK856_11040"
     CDS             1444..>2005
                     /locus_tag="PK856_11040"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HRL02943.1"
                     /translation="MTWLVLMSLGYAISVWGLSDQRIQSALQQAMPTGRTIQIGEITN
                     RGWLPYPNVTLHNVSVSYPNRTTAQFKLKELHITLNWNSLFGHAKVKKLRLLQPEVTV
                     ARMHDNTWNIDDFYQNPRATSGIDDLEIIAGVLKVQETQHAYELSNVNASIESWQKPQ
                     SALHLAFDWNSTLMGKQQISLEAPIQK"
BASE COUNT          567 a          482 c          453 g          503 t
ORIGIN      
        1 ggccaaagca aacaggctgt cgcaaaatgc cctgacccaa gcctcgtcca attgtggcaa
       61 tgccacgctc aaagtggccc acttgggtgt ggcacccatc gctgccatgt cggacaaatt
      121 caccgccaaa accttgtgcg ccaagtcggc aggctgtaca tcggtgaaaa aatggcggcc
      181 acccaagagc atgtcgctgc tgatgtggat gtcgcaatcg gcttgcggac ggatgatggc
      241 ggcatcatcg cctatgccca ataccacttg ggcatcgttg tcttgtctgg cggctaagta
      301 ttgttttata aaatcaaatt ctttcatgcg tcatccaaat tgaatggttt acaagcgggc
      361 atttcttatt tacaggccgc ttatattcca aaaacatgcg tgttttttat gtgagcttaa
      421 gtaaacattg tactgcattg caacgtaagc gccctaaaaa gcgctataat cgcgctttct
      481 ttttgttcca cacaagcttg gtaaagagac caccaccatg agcgacttag atttacaaaa
      541 aatttattca ggtaaagttc gcgacttata cgccatcgat gacaaacgca tgttaatggt
      601 tgccaccgac cgtttatctg cttttgacgt gattttggat gagcccattc caaccaaagg
      661 tgaaattttg acgcaaattt ccaatttttg gttcgactat ttaaaagacg tgatgcccaa
      721 tcaatttatt ggacaaaccg tttatgatgt gttgccagaa gcccaagcac gtgagtatga
      781 aaaacgcgcc gtggtggtga aaaaattgac ccccatcaaa atcgaagcca ttgtgcgcgg
      841 ttatttggct ggtagcggtt ggaaagacta tcaaaacaca ggcaaattgt gtggcataga
      901 cttgccagca ggtttgcaac aagcgcaaca attgcctgaa gtggtgttta ccccttcaac
      961 caaagccgct gtgggcgaac acgacgaaaa catttctttt gatgaagccg caaaaatcat
     1021 tggtgaagac ttggcgcacc aagtgcaaac caaagcaatc gagttgtaca aaaaagccgc
     1081 tgcctacgct gaaagccgtg gcatcatcat ttgtgacacc aaatttgaat ttggtacaga
     1141 tgaaaacggc actttgattt tgatggatga agtattgacc cctgattcaa gccgcttctg
     1201 gcctgcagac caatacgaag tgggcaccaa ccctccctca ttcgacaaac aatttgtccg
     1261 tgactggtta gaagcttcag gctggaacaa gcaagcacct gcaccgaaag ttcctgcaga
     1321 cgtgattgcc aaaaccgtgg ccaaatatga agaagctttg cagttgttga ccaagtaatt
     1381 gttgcaatga tcaggaagtt acgttcgggg aagttttggc ttaagctgat ttttggtggc
     1441 tttgtgactt ggctggtgct gatgtcctta ggctatgcca tttcggtgtg gggtttgagt
     1501 gaccagcgca ttcaatcggc tttacaacaa gccatgccta caggccgcac catccaaatc
     1561 ggtgaaatca ccaaccgcgg ttggctgcct tacccgaatg tgactttaca caatgtcagc
     1621 gtttcctatc ccaaccgcac caccgcccaa ttcaaactca aagaattgca catcacctta
     1681 aattggaaca gtttgtttgg ccatgccaaa gtcaaaaaac tgcgcctgtt gcagcccgaa
     1741 gtcactgtcg cccgcatgca cgacaacact tggaacattg atgattttta ccaaaatcca
     1801 cgcgccacct caggcataga cgatttggaa atcatcgcgg gtgtgctaaa agtacaagaa
     1861 acccaacatg cttatgaatt aagcaatgtc aatgcatcga tagagagttg gcaaaagcca
     1921 caaagtgcct tgcacttggc gtttgattgg aattctaccc tcatgggcaa gcaacaaatc
     1981 agcctagagg cccccattca aaaaa
//
LOCUS       DAOMVK010000419         1995 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_16667_length_1995_cov_1.727835, whole genome shotgun sequence.
ACCESSION   DAOMVK010000419 DAOMVK010000000
VERSION     DAOMVK010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16667_length_1995_cov_1.727835"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1345)
                     /locus_tag="PK856_11045"
     CDS             complement(<1..1345)
                     /locus_tag="PK856_11045"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRL02944.1"
                     /translation="MDKPGNYYQGINQSLLHHVPETAVRILEIGCATGVFGAALKELN
                     PKRYVVGVEYVPEVAEVARTHLDEVYAFDIQTHELPDSIELQSFDCIVCGDVLEHLTE
                     ATSVLRKLLPYLKPDGKVVACIPNVQHFSVIQQLLTQNFQYHGVGLLDKTHVNLMTLP
                     NMFKMFLDAGYFPQLAQVLRVGAVDEGFMHSLLPVLGYLGVAPERAKSALEAYQYVLT
                     ASIQQANINRAETWASEIKPQACTVAVAMSNPLQYQNNVAASPELASNDMGHQLLPMT
                     GAQNMAQAWEHAKLHAAHDWIVLVHQDVYLPTDWLKQVQYAWHEAEAELGQQIGVLGV
                     VGMRNLGDETVSYLGRCIDRVATYLPAASLPSLTDSLDELVLVYRKDRFLPVISPELG
                     FHFYGTDSVLKAQEAGFAAAVVDVPCFHNSSRFGSHMNDEDLKQKAMAFHQLWQHR"
     gene            complement(1416..>1995)
                     /locus_tag="PK856_11050"
     CDS             complement(1416..>1995)
                     /locus_tag="PK856_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3482 domain-containing protein"
                     /protein_id="HRL02945.1"
                     /translation="QRERHFQQDLLQAFRFYQQADLPADWMPKAFSQDPFDPELLKQF
                     GLRTSTGAAVGALLGLGVDAATAGLSGGMGMALGGVIGGVLSNMGSLSDRISGVKTIH
                     IDPATITLLATRAQALILALLQRGHAAQQALSLQAADVIWPNSNLPSPLRRARSHEEW
                     SALNGVKEAKAQTNRREAAQELQQKLSRPLSS"
BASE COUNT          502 a          536 c          460 g          497 t
ORIGIN      
        1 ggcggtgttg ccacaattga tgaaacgcca tggctttttg tttcaaatcc tcatcattca
       61 tgtgactgcc aaaacgcgat gaattgtgaa aacacggcac atcaaccact gcagctgcaa
      121 agccagcctc ttgagcctta agcaccgaat cggtaccgta aaaatgaaag cctaattccg
      181 gtgatattac aggcaagaat cggtctttgc gatacaccaa taccaactca tccaaggagt
      241 cggttaacga aggcaaagac gcagcgggta agtaggtcgc aacccgatct atgcaacgcc
      301 ccagataact gacagtttca tctcctaggt tacgcatacc taccacaccc aatacgccta
      361 tttgttgccc taactcagcc tcagcctcat gccaagcata ctgaacttgt ttcaaccaat
      421 ccgtaggcag atacacatct tggtgcacca acacaatcca atcgtgcgca gcatgcagtt
      481 tggcatgttc ccaagcttgc gccatgtttt gcgcccctgt catgggcagc aattggtgcc
      541 ccatgtcatt gctcgccaat tcaggtgagg ctgcaacatt gttttggtat tgcagtgggt
      601 tgctcatcgc caccgctacc gtacaggctt gtggtttaat ttcagatgcc caagtctcag
      661 cccgattgat gttggcttgc tgtatgctgg cagtcaacac atattgatag gcttccaatg
      721 ccgatttcgc gcgttcaggc gctacaccca aataccccag cacaggcaat aaactgtgca
      781 tgaagccttc atctaccgca ccaacacgta gcacttgcgc caattgtgga aaataacccg
      841 catccaaaaa cattttgaac atattgggca aagtcatcaa attgacatgg gttttgtcca
      901 acaagcccac accatggtat tgaaagtttt gcgtcagtaa ttgttgtatc actgagaagt
      961 gctgcacatt cggaatacac gccaccactt tgccatcagg cttcaaatac ggcagcaatt
     1021 tgcgcaacac agatgtggct tcagttaggt gctccaatac atcaccgcaa acgatacaat
     1081 cgaacgattg caactcaata ctgtcaggca attcatgggt ttgaatatca aaggcataca
     1141 cttcatccaa gtgtgttctg gcgacttctg ctacctctgg cacatattcc acccccacca
     1201 cataacgctt tggattgagt tcttttaatg ccgcaccaaa caccccagtg gcacatccaa
     1261 tttccaaaat acgcacagct gtttcaggta cgtgatgaag caacgattga ttgatgcctt
     1321 gataataatt gcctggctta tccatgaaat ccccttggtt tattattgca ctgcttgtcg
     1381 tttttgcatg gcacagaata gcattgttac ccactttatg acgacagggg ccggcttaat
     1441 ttttgttgca attcttgcgc ggcttcgcgg cggttggttt gggctttggc ttctttcacg
     1501 ccattcaagg ccgaccattc ttcatggctg cgggcgcggc gtaaggggct gggtaaattg
     1561 ctgttgggcc aaatcacatc agcggcctgt aaactcaagg cttgctgtgc ggcgtggccg
     1621 cgttgcaaca aggccaaaat caaagcctgt gcgcgggtag cgagcaaggt gatggtggct
     1681 gggtctatgt gtatggtttt gacgccactg atacggtcgg acaagctgcc catattggac
     1741 aacacgccgc ctatcacgcc gcccaatgcc atgcccatac cgcctgataa gcccgctgtg
     1801 gcagcgtcta cgcccaagcc taacaaagcc cccacggctg cgcccgtact ggtgcgcaaa
     1861 ccaaattgct tcagcaattc ggggtcgaac gggtcttggc tgaaggcctt gggcatccaa
     1921 tcggcgggca aatcggcttg ctggtaaaac cgaaatgcct gtaacaaatc ttgttgaaaa
     1981 tggcgctcgc gttgg
//
LOCUS       DAOMVK010000420         1994 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_16679_length_1994_cov_2.520371, whole genome shotgun sequence.
ACCESSION   DAOMVK010000420 DAOMVK010000000
VERSION     DAOMVK010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16679_length_1994_cov_2.520371"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="PK856_11055"
     CDS             complement(<1..426)
                     /locus_tag="PK856_11055"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRL02946.1"
                     /translation="MLTHLKLKTKLSWQTNSVMLLLCTMCLGINTASAAPQNLSPNPA
                     TPPAQQGVEWNNQGFVLGQQGQYAQATALYQRALPLLQASDHRTQLNRAATLNNLGVA
                     LSHQQQLNAAAQAFSEAYTLRAHHLSPTAAPALLSQHNLA"
     gene            605..1375
                     /locus_tag="PK856_11060"
     CDS             605..1375
                     /locus_tag="PK856_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02947.1"
                     /translation="MAYSRRTVLKLVLGGAALSQAACINLSPFGTRQPATSRSERLDY
                     GQLKIYYNDDVVVYLTREFHLVFKDVEYHFKFRKLPLAMLNKAFATIGNVSVGTVSDN
                     GRLAYGNAQSITLTFRADDLTAAEKQIMASMKVREDEAGGSAYTNPLINHGIVKAKQA
                     RYLTFIKLSSDFNNVYLNVSYPEGFFDDALKPNLVAHMRWDIPLDAARAKQQQETLSR
                     AKSPIKIGADGRVFYKGKPMRNLADDITFVDIRTRDHM"
     gene            1489..>1994
                     /locus_tag="PK856_11065"
     CDS             1489..>1994
                     /locus_tag="PK856_11065"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRL02948.1"
                     /translation="MYFAHANGFPAACYPAFFAPLAARYEVKYLPVLAMNPDYPVTPT
                     WHLLVDEVIADMVAQACGSKVVGVGHSFGSLLLLMASYKRPDLFEQVVLMDPPFLMGK
                     KTALLEVAQKFKLSMVDRATPALLAVKRVDFWLSRDVAYQSLRHKSIFKRFDERCFQA
                     YIAHGLVE"
BASE COUNT          449 a          438 c          582 g          525 t
ORIGIN      
        1 ggcgaggttg tgttgtgaca ataaggcggg cgcggcggta gggctcaaat ggtgcgcgcg
       61 caaggtgtat gcttcgctaa aagcttgtgc tgcagcgttt aattgctgtt ggtggcttaa
      121 ggccacgccc aagttgttta aggtggcggc acgattgagt tgggtgcggt ggtcgctcgc
      181 ttgcagcagt ggcaaggcgc gctgataaag tgcggtggct tgggcgtact ggccttgttg
      241 gcctaagaca aaaccctgat tgttccattc aaccccttgt tgtgcgggtg gggtggcagg
      301 gtttggggac aggttttggg gcgctgcgct ggcggtgttg atgccgagac acatggtaca
      361 cagcaagagc atcacgctgt ttgtttgcca agaaagtttg gttttcagtt tgaggtgcgt
      421 cagcatgcag tgcccatttt gtacatatta agcatgaggc taatgccaaa caagcggcct
      481 gtaaaggctt ggggctggca caatgttgtg ttttggcgcg tgttatcggc ttttgtggcg
      541 tgaaaactca cagactgtgt tggccaaggt atattgaaac acacgcagac aacaaggaaa
      601 tgacatggct tactccagac gcacggtttt aaaactggtt ttgggcggcg cggctttgag
      661 ccaagcggcc tgtatcaatt taagcccgtt tggtacgcgc caacctgcca cttcgcgctc
      721 agagcgtttg gattatgggc aattgaaaat ctattacaac gatgatgtgg tggtgtattt
      781 gacccgcgag tttcatttgg tgttcaaaga tgtggaatac catttcaagt ttcgcaagct
      841 gccgcttgcc atgctgaata aggcttttgc cacgattggc aatgtcagtg tgggcacggt
      901 gagcgataac ggccgtttgg cctacggcaa tgcgcaaagc attactttga cgtttcgtgc
      961 ggacgatttg acggcggcag aaaaacaaat catggccagc atgaaggtgc gcgaggatga
     1021 ggcaggcgga agcgcgtaca ctaatcccct gattaaccat ggcatcgtta aagccaagca
     1081 agctcgctac ctgacgttta tcaagttaag cagtgatttc aacaatgtgt atttgaacgt
     1141 gagctatccc gaaggctttt ttgacgatgc cttaaagccg aatctcgttg cccacatgcg
     1201 ctgggacatt cctttggatg cggcaagggc gaagcaacaa caagaaacgc tgagccgtgc
     1261 caaatcgccg attaaaatag gcgcagatgg acgggtgttt tacaaaggca agcctatgcg
     1321 caatttggcc gatgacatca cctttgtcga catacgcacg cgcgaccata tgtgagggta
     1381 gcgcagggta agtaaaatga gccagcgcta ggtaagtctg aaaatccacg gtatgatggc
     1441 tgccatattt cacacctttc tttctcaatg aatccaagca aacccatctt gtattttgcc
     1501 catgccaatg gttttcctgc cgcttgttat cctgcttttt ttgcgccttt ggcggcgcgg
     1561 tatgaggtga aatacctgcc tgtgttggcg atgaatcccg attatcctgt cacacctact
     1621 tggcatttgt tggtagacga ggtcattgca gacatggtgg cacaagcctg tggcagcaaa
     1681 gtggtgggcg tggggcattc gtttggttca ttgctgttgt tgatggcgtc ttacaagcgg
     1741 cctgatttgt ttgagcaagt ggtgttgatg gatccgcctt ttttaatggg caagaaaacc
     1801 gctttgcttg aggtggcgca aaaattcaaa ctcagcatgg tagaccgcgc cacgcccgcg
     1861 ttgttggcgg tgaaacgggt ggatttttgg ctcagccgtg acgtggccta ccaaagcctg
     1921 cgccacaaat ccatcttcaa gcgttttgat gagcgttgtt ttcaggctta catagcgcat
     1981 ggcttggtcg aaaa
//
LOCUS       DAOMVK010000421         1992 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_16705_length_1992_cov_2.721218, whole genome shotgun sequence.
ACCESSION   DAOMVK010000421 DAOMVK010000000
VERSION     DAOMVK010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16705_length_1992_cov_2.721218"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /gene="phnH"
                     /locus_tag="PK856_11070"
     CDS             <1..632
                     /gene="phnH"
                     /locus_tag="PK856_11070"
                     /inference="COORDINATES: protein motif:HMM:NF017645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HRL02949.1"
                     /translation="RGLMQNQLIAGFQNPVADAQTVFRTALCAMSEPGLPQNLPMVAG
                     LDSLQAATWAVLQTLLDADVKVYLSDSVSTEKVRSNVLFHTGCELTNDKAQADFIVCD
                     LATALTWDWRSLKRGSERSPEHSATLIIQVEALQGDSHSVWQGPGIEHQRDMHTVSDK
                     TFWLKRAAAMAFPLGVDVMLVAGQDLIGLPRTTQVQFEPVTEQGAASCM"
     gene            623..1741
                     /locus_tag="PK856_11075"
     CDS             623..1741
                     /locus_tag="PK856_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HRL02950.1"
                     /translation="MYVAVKGGEQAIKSAHQLLAEYRRGDAAVAEISVAQIKQQMPLA
                     LARVMTEGSLYDEELAALAVKQAAGDITEAIFLLRAYRTTLPRFGDAEPLNTREMLLS
                     RRISAIYKDLPGGQLLGPTFDYTHRLLDFALLAEGETDANKRPVNLDNKQTTACPHVM
                     NILEQEGLMAPPECNNAKHSDITREAVQFPTGRSERLQILARGDEGYLLSLAYSTQRG
                     YGRNHPFVGEIRIGEVEVAFTPPELGFEIVLGAIEVSECEMVTQFVEEAGADEVVFTR
                     GYGLAFGQAERKTMAMALMDRALCADEYGEEITSPAQQQEFVLAHCDNVEAAGFVSHL
                     KLPHYVDFQSELELIRKIRRDQAGASHAEEDANEVEPA"
     gene            1738..>1992
                     /locus_tag="PK856_11080"
     CDS             1738..>1992
                     /locus_tag="PK856_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HRL02951.1"
                     /translation="MNPHTDRQDYNFAYLDEQTKRMIRRGLLKAVAIPGYQVPFGGRE
                     MPLPYGWGTGGIQVTASILGADDVLKVIDQGADDTTNAVSI"
BASE COUNT          494 a          532 c          546 g          420 t
ORIGIN      
        1 ggagaggact gatgcaaaac caattgatag ctggctttca aaaccccgta gccgatgccc
       61 aaacggtgtt tcgcaccgcc ttatgcgcca tgagcgaacc aggcttgcca caaaatcttc
      121 ccatggtagc aggcttagac agcttacagg ccgctacttg ggcggtgtta caaaccttgc
      181 tcgatgccga tgtcaaagtg tatttgagcg acagcgtgag cacagaaaaa gtgcgcagca
      241 atgtgttgtt tcacactggt tgtgaactca ccaacgacaa agcccaagcc gatttcattg
      301 tgtgtgattt ggccaccgct ttgacttggg attggcgcag tcttaaacgc ggtagcgagc
      361 gctcgcctga gcattcggcc acgctcatca tccaagtcga ggccttacaa ggcgattcgc
      421 acagcgtgtg gcaaggccct ggcattgagc atcagcgcga catgcatacc gtatcggaca
      481 agactttttg gctcaaacgc gccgccgcca tggctttccc attgggcgtg gacgtgatgt
      541 tggtggcagg acaagacctc atcggtttgc cacgcaccac gcaagtgcaa tttgaacctg
      601 taaccgaaca aggagcggca tcatgtatgt agccgtgaaa ggtggcgaac aagccatcaa
      661 aagtgcccac caattgttgg ccgaataccg ccgtggtgat gctgctgtgg ccgaaatcag
      721 cgtggcacaa atcaagcaac aaatgccctt ggcgctggcg cgggtgatga ccgaaggctc
      781 actttacgat gaagaattgg ccgctttggc ggtgaaacag gccgcaggcg acatcaccga
      841 agccatcttc ttgttgcgcg cttaccgcac caccttgcca cgctttggcg atgccgagcc
      901 tttgaataca cgcgaaatgc tgctcagccg ccgcatttct gccatttaca aagacttacc
      961 aggcggccaa ttgctcggcc caacctttga ctatacccac cgcttattgg actttgcttt
     1021 gttggcagag ggtgaaacag acgcaaataa gcggcctgta aacttggaca acaagcaaac
     1081 cactgcctgc ccacacgtga tgaatatttt ggagcaagaa ggcttgatgg caccgcctga
     1141 gtgcaacaat gccaaacaca gcgacatcac ccgcgaagcg gtgcagtttc caacaggccg
     1201 ctctgagcgt ttgcaaattt tggcacgtgg cgacgaaggc tatttgctgt ctttggcata
     1261 ttccacccag cgtggctatg gccgcaatca cccgtttgtc ggcgaaattc gcatcggaga
     1321 agtcgaagtg gcctttacgc cacctgagtt gggttttgaa atcgtgctcg gtgccatcga
     1381 agtgagcgaa tgtgaaatgg tgacgcagtt tgtggaagaa gcaggggcag acgaagtggt
     1441 gtttacccgc ggctatggtt tggcatttgg acaagccgag cgcaaaacca tggcgatggc
     1501 cttgatggac cgcgctttgt gcgccgatga atacggtgaa gaaatcacct cacctgcgca
     1561 gcaacaagaa tttgtgttgg cgcattgtga caatgtggag gcagcaggct ttgtgtcgca
     1621 cctgaaattg ccacattacg tggacttcca atcagagttg gaattaatcc gcaaaattcg
     1681 ccgtgaccaa gcaggcgcaa gccacgcaga agaagacgcc aacgaggtag aacccgcatg
     1741 aatccgcaca cagacagaca agactataac ttcgcgtact tggacgaaca aaccaagcgc
     1801 atgatacgcc gcggcttgct caaagccgtt gccatccctg gctaccaagt gccgtttggc
     1861 ggccgcgaaa tgccactgcc ttatggttgg ggcacaggtg gcatccaagt gacggcgagt
     1921 attttgggcg ctgacgacgt gctcaaagtg atagaccaag gcgcagacga caccaccaat
     1981 gccgtgtcca tc
//
LOCUS       DAOMVK010000422         1988 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_16748_length_1988_cov_4.226591, whole genome shotgun sequence.
ACCESSION   DAOMVK010000422 DAOMVK010000000
VERSION     DAOMVK010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16748_length_1988_cov_4.226591"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..544)
                     /locus_tag="PK856_11085"
     CDS             complement(11..544)
                     /locus_tag="PK856_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRL02952.1"
                     /translation="MSDFILIDDNATFARLLKRGMERQGHSLTWCENAAAALDLQNAG
                     DGIILDLNLGEDSGLKLLPELHQRFPNTKIVILTGYASINTAVSAIKLGACEYLPKPA
                     DIDTILHCFFKDPIAQETLAETPLENDPLSLKRLTWEHLQRVLEEHDGNISATARALN
                     MHRRTLQRMLQKHPVKK"
     gene            complement(537..1769)
                     /locus_tag="PK856_11090"
     CDS             complement(537..1769)
                     /locus_tag="PK856_11090"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRL02953.1"
                     /translation="MIKSLLLDEERLAQLNIHIRWLFTFLAISLWAVCWLAKVPLPHV
                     LIAQAIATLAMGNTLIWVWQKSASAHPAVSIPVGLVLDTWSLTELLFLTGGASNPLTF
                     LYLVPVLFAALMCSARFAWTLTGFTIITYLLIYFFHLPVLSPHWLHQNLQLHFIGMWL
                     AFGLISTLVCVLVTWLMQNLKEREVLLRLAYRNQMRDENMLWLGMNAANLAHKMSTPM
                     NNLYLLLDELRQDARPQDADDLALMRTQLEECTDILHSLRRDAKREPSEVVFEEAFAI
                     HLARWQNLRPQVEVNWQQRQTAPNPMTVDPLFWPAVFNILNNAADAGDNHVEVISQIQ
                     DNTLVIEIINRSGSLPQAQLEWAGLSALSSQKPSGLGLGMKLSHATFEKLGGSLSLSN
                     QQQGGVRAVISLPLARHE"
BASE COUNT          492 a          469 c          522 g          505 t
ORIGIN      
        1 tggggtgcgc ttattttttc acgggatgtt tttgcagcat gcgctgcaag gtgcgtctgt
       61 gcatgttgag ggcgcgggcg gtggcggaga tgttgccgtc gtgttcttcc aacacgcgtt
      121 gcaagtgctc ccaagtgagg cgtttgaggg agagtgggtc gttttccaat ggggtttcgg
      181 ccaaagtttc ttgggcgatg gggtctttga aaaagcaatg caagatggtg tcgatgtcgg
      241 cgggcttggg caggtattcg cacgcgccaa gcttgatggc gctgacggcc gtgttgatgc
      301 tggcatagcc tgtgaggatg acgattttgg tattggggaa gcgttgatgc agctctggca
      361 acagtttcag gccgctgtct tcgcccaaat tcaagtccaa aatgatgcca tcgcctgcgt
      421 tttgcaaatc caatgcggcg gcggcatttt cacaccaagt gaggctgtgg ccttgccgtt
      481 ccatgccgcg tttcagcaag cgggcaaacg tagcattgtc atcgattaaa ataaaatcac
      541 tcatgtcttg ctagcggtaa gctgatcaca gctcgcacgc ctccttgttg ttggttggac
      601 aaactcaagc tgccaccgag tttttcaaat gtggcgtgtg acaatttcat gcccaagcct
      661 aagcccgatg gcttttgtga tgacaaggca ctcaaacccg cccattccag ttgggcttgc
      721 ggcaaggagc cgctgcggtt gatgatttct atcaccagcg tattgtcttg gatttgggag
      781 atgacttcca catggttgtc gcctgcatcg gcggcattgt tcaaaatatt gaacacggcc
      841 ggccaaaaca acgggtctac ggtcatgggg ttgggggcgg tttgacgctg ttgccagttg
      901 acttcaacct gtggtcgcaa attttgccag cgtgccaaat ggatggcaaa ggcttcttca
      961 aacaccactt ccgacggctc gcgtttggca tcgcggcgca gactgtgcaa gatgtcggta
     1021 cattcttcca attgcgtgcg catcaaggcc aaatcatcag cgtcttgtgg gcgtgcatct
     1081 tgtcgcaatt catccaataa caaatacaaa ttgttcatcg gtgtggacat tttgtgcgcc
     1141 aaattggcgg cattcatgcc cagccacaac atgttttcat cgcgcatttg gttgcggtaa
     1201 gccaagcgca gcaacacttc gcgctctttt aagttttgca tcaaccatgt caccaacacg
     1261 cacaccaagg tagaaatcaa gccaaaagcc aaccacatgc cgatgaaatg caattgcaaa
     1321 ttttggtgta gccaatgtgg gctgagtacg ggcagatgga aaaaataaat cagcaaataa
     1381 gtgatgatgg taaagcctgt cagtgtccaa gcaaagcggg cagaacacat taaagccgca
     1441 aacaacacgg gcaccaaata caaaaaagtc agtggattgc tggcaccgcc agtcaaaaac
     1501 aacagctcag taagcgacca agtgtccaat accaaaccga ctgggatgct cacggcggga
     1561 tgggctgagg cacttttttg ccaaacccat atcaaagtat tgcccattgc caaggtggca
     1621 atggcttgcg caatcaagac atggggcagg gggactttgg ctaaccaaca cactgcccac
     1681 aggctgatgg ccaaaaaagt gaacagccaa cgaatgtgta tgttcaattg cgcgaggcgc
     1741 tcttcgtcca ataataagct tttaatcatg gtttaacaca ttgaatccca atgaagtatt
     1801 tgtttgctgc ggcaatatgt cgcattgttt ttacaggccg gcttactgat aatgaagtct
     1861 tcatttgatt tacatcaatg agcacataat aaaacaaaca ctcacgaggt acacatcatg
     1921 tttgattggg acgcttttat gctggcgcgc attcagttcg cgtttacggt ctcgtttcac
     1981 atcatttt
//
LOCUS       DAOMVK010000423         1977 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_16848_length_1977_cov_3.149324, whole genome shotgun sequence.
ACCESSION   DAOMVK010000423 DAOMVK010000000
VERSION     DAOMVK010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16848_length_1977_cov_3.149324"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..984)
                     /gene="rsmI"
                     /locus_tag="PK856_11095"
     CDS             complement(109..984)
                     /gene="rsmI"
                     /locus_tag="PK856_11095"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRL02954.1"
                     /translation="MLQPIIERAQERLEPQTLYVVATPIGNLSDITLHALAVLSKADV
                     VCAEDTRVTGQLLAAYGIKAKLISVREQNEQTMAVKVIEALAAGQIVAQVSDAGSPAV
                     CDPGARLVAQVRAAGYKVSPVVGASAVVAALSVAGITAPDFVFAGFIPSKPSERRTLL
                     NTWQNIHQTIVSYETPHRIEATLADMQAVFPERVVMLAREITKTFETFLIGTPSELQA
                     ALTQDSNQSRGEMVLIVHPLEKVQEDTIVPEAEQLLSLLLDDMPLKKACDIVHRTFSA
                     NKKHLYDLGLTLKSK"
     gene            1040..1390
                     /locus_tag="PK856_11100"
     CDS             1040..1390
                     /locus_tag="PK856_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRL02955.1"
                     /translation="MRLQHPAGLAAEEQAARFLMMQGCRIVVRNWHCKSGEIDLVVWD
                     GEVCVFVEVRMRSSEQFGGASASITASKQRKLLLSAQTYLQAEHSSEPECRFDAVLIN
                     GEGHIEWLKNIIGM"
     gene            1391..>1977
                     /locus_tag="PK856_11105"
     CDS             1391..>1977
                     /locus_tag="PK856_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HRL02956.1"
                     /translation="MNLSDRVFDHFAESIKAKQDNQELLAEATAAAIELMFAALVNDG
                     KVLACGNGGSAADAQHFAAELVGRFEMERMGLAGIALTTDTSALTAIANDYDFNRVFS
                     KQVEALGRSGDVLLAISTSGNSANIIAAIEAAHEHDMRVVALTGKDGGQIAHILQDQD
                     ILLNVAHQRTARIQEIHILLIHAICDGIDQLLMGNV"
BASE COUNT          470 a          441 c          521 g          545 t
ORIGIN      
        1 tttgagtccc ctgcgtctac caatttcgcc acctgggcag tggatttggc ttactaaggc
       61 tgtgcattat agacggataa attttcttgt caataatctg tcacacattt atttagactt
      121 taatgttaag cctaagtcgt ataagtgttt tttattggca gaaaacgtgc ggtgcacaat
      181 atcgcaggct tttttgagcg gcatgtcgtc cagtaacaag cttaataatt gctcagcttc
      241 ggggactatg gtgtcctctt gtactttttc cagtgggtga acaatcaata ccatttcacc
      301 gcggctttga ttgctgtcct gtgtcaaagc ggcctgtaat tcgcttggtg tgccaatgag
      361 gaaggtttca aaggttttgg tgatttcacg cgccaacatc accacccgct cgggaaatac
      421 ggcttgcatg tcggccaaag tggcttcaat gcggtgtggg gtttcgtagc tgacaatggt
      481 ttggtgaatg ttttgccaag tattgagtag ggtgcgacgt tcactcggtt tggatggaat
      541 aaagcccgca aacacaaaat caggtgcggt aattcctgcc acagacaaag ccgccaccac
      601 agcgctggcg cccaccacag gcgatacttt atagcctgct gcacgaactt gtgccaccaa
      661 acgggctcct gggtcgcaca cggcaggtga gcctgcatcc gacacttgcg ccacgatttg
      721 ccctgcagcc aaagcctcta tcactttcac tgccatggtt tgttcatttt gctcacgcac
      781 actgatgagt ttggctttga tgccatatgc tgcgagcaat tgccctgtga cccgtgtgtc
      841 ttcggcgcac accacatctg ctttgcttaa aactgcaaga gcatgtaagg taatgtcgga
      901 taaattgcca atcggtgtgg ccaccacata taatgtttgt ggctctaagc gctcttgggc
      961 gcgttctatt atgggttgta acacaggcag tctcatgggg catttcaatg gtgtgatact
     1021 atcatatgcg gagcgtgaca tgcgtttaca gcacccagca ggtttggcgg ccgaagagca
     1081 ggctgcaagg tttttaatga tgcaaggttg tcgcattgtg gtgcgaaatt ggcattgtaa
     1141 gagtggcgag attgatttgg tggtttggga tggtgaggtg tgcgtgtttg ttgaagtgcg
     1201 catgcgcagt tctgaacaat ttggtggcgc atcagcttcc attaccgcca gtaagcagcg
     1261 taaattgctg ttaagcgcgc aaacctatct gcaagcagag cactcaagcg agccagaatg
     1321 ccgttttgat gcggtgttaa tcaatggcga aggtcacatc gagtggctca aaaacataat
     1381 tggaatgtaa atgaatttaa gcgatcgcgt atttgaccat tttgcggaaa gcattaaggc
     1441 gaaacaagac aaccaagaat tattggctga ggccactgcc gctgccatcg aattgatgtt
     1501 tgccgctttg gtaaacgatg gcaaggtatt ggcttgcggc aatggtggct cggctgccga
     1561 tgctcagcat tttgctgccg aattggtggg tcgttttgaa atggagcgca tgggcttggc
     1621 gggcatcgcc ttaaccactg atacttctgc tttaaccgca attgccaacg attacgattt
     1681 taaccgcgtg ttttccaaac aagtggaagc cttgggacgc agtggcgatg tgttgttggc
     1741 gatttcaact tcaggtaatt cagccaacat cattgccgca attgaagccg cgcatgagca
     1801 cgacatgcgc gtggtggctt taacaggcaa agatggcggt caaattgcgc acatcttgca
     1861 agaccaagac attttattga atgtggcgca tcaacgcacg gcgcgcattc aagaaatcca
     1921 tattttgtta atccacgcca tctgtgatgg gattgatcaa cttttaatgg gcaatgt
//
LOCUS       DAOMVK010000424         1975 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_16874_length_1975_cov_2.730729, whole genome shotgun sequence.
ACCESSION   DAOMVK010000424 DAOMVK010000000
VERSION     DAOMVK010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16874_length_1975_cov_2.730729"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PK856_11110"
     CDS             complement(<1..586)
                     /locus_tag="PK856_11110"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HRL02957.1"
                     /translation="MDLYLASGSPRRAQLLAQIGFSISKIASDIDETPQEGELALAYT
                     ERMALQKSAQALALFSATYGTQPELPVLTADTSVAIDGLILGKPDNHAHAVEMLRMLS
                     GRSHQVISAVVVWHQGQRSQATQVSDVTFRPLTEAEIQAYVNSGEADDKAGAYGIQGA
                     AAVFIEHLSGSFSGVMGLPLFETAALLRQLNVHKQ"
     gene            complement(589..1413)
                     /locus_tag="PK856_11115"
     CDS             complement(589..1413)
                     /locus_tag="PK856_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRL02958.1"
                     /translation="MKSIFITGCSSGIGYATAHLLKQQGWRVIASCRQVADVARLQSE
                     GLECLQVDVRVSADYQAAIAYIKEHVGTLDAFFANAGYGQNGAIDDIPLDAVKAQFAT
                     NVFGTWDGILHVLPVFRDQGFGRILVNSSVLGFAAMALRAPYNSSKFALEGLCDTLRL
                     ELAATDIHVSLLQPGPIKTRFRHNTLEQFLANVNQENSHANYQFLLQRLRGEGKPNRF
                     AISSEDCAVVCAQALNAPHPKARYRVTTVTKMMTVFKHLLPTCILDKILIAGNDKE"
     gene            1495..1791
                     /locus_tag="PK856_11120"
     CDS             1495..1791
                     /locus_tag="PK856_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02959.1"
                     /translation="MKSWFNPSATQNRARAWRFAKSLRDAQLHPWKAEEVALAEQFIA
                     ETLDADGYRAAVYALSDWHNIKDRADFGGYSKAERDYRADIIATMEITIAQSEK"
     gene            1791..>1975
                     /locus_tag="PK856_11125"
     CDS             1791..>1975
                     /locus_tag="PK856_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HRL02960.1"
                     /translation="MADLWWQWLGLWFSAFSSATILPGTSDVVLVAMVNAHPQWWAWA
                     WLVATVGNGMGGYASYW"
BASE COUNT          437 a          479 c          572 g          487 t
ORIGIN      
        1 attgtttatg cacgttcagt tggcgtaaca gggcagcggt ttcaaacaaa ggcaagccca
       61 tcacgccgct gaagctgccg cttaagtgct cgataaacac cgctgctgcg ccttgtatgc
      121 cgtaagcgcc tgctttgtca tcggcttcgc cgctgttcac ataggcttga atctcggctt
      181 cggtaagcgg cctgaaggtg acatcgctga cttgtgtggc ttggctgcgt tggccttggt
      241 gccacacgac aacggcgctg atgacttggt gggagcggcc tgaaagcatg cgcagcattt
      301 ccactgcatg ggcatggttg tcgggtttgc ccaagataag cccatcgatg gcaactgagg
      361 tatcggcggt caatacgggc aattctggct gcgtgccata ggttgcgctg aataaagcca
      421 aagcctgtgc tgatttttgt aaggccatgc gttcggtata ggccagggcc aattcgcctt
      481 cttgtggtgt ttcatcgatg tcgctggcga ttttgctgat gctgaagcca atttgtgcca
      541 acaattgggc gcgtctggga ctgccagagg ccaaatacaa atccatgttt attccttgtc
      601 attgcctgcg atgaggattt tgtccaagat gcaggtggga agcaggtgtt taaataccgt
      661 catcattttg gtgacggtgg tcacgcggta gcgtgctttg gggtgagggg cattcaaggc
      721 ttgtgcgcaa accacggcgc agtcttcgct ggagatggca aagcgattgg gcttgccttc
      781 gccacgcagc cgttgcaata aaaattggta attggcatgg ctgttttctt ggttcacatt
      841 ggccaaaaat tgttccaatg tgttgtggcg aaaacgggtc ttgatgggcc caggttgtaa
      901 taggctgacg tgaatgtcag tggcggccaa ttccagtctt aaggtgtcgc acaagccctc
      961 aagggcgaat ttgctgctgt tatagggcgc gcgcagtgcc atggcggcga aacccaaaac
     1021 actggaattg accaaaatgc ggccgaaacc ttggtcgcga aatacgggca gcacatgcaa
     1081 aatgccatcc caagtgccga acacattggt ggcaaactgg gctttcaccg catccagtgg
     1141 aatgtcgtct atcgcgccat tttggccgta gcccgcgttg gcaaaaaacg catccagtgt
     1201 gccaacatgt tctttgatgt aggcaatggc ggcttggtag tcggccgaaa cgcgcacatc
     1261 cacttgcaaa cactccaagc cttcgctttg caaacgcgcc acatcggcca cttggcggca
     1321 gctggcaatc acgcgccagc cttgttgttt taataagtgg gcggtggcat aacctatgcc
     1381 gctggagcaa cctgtgatga agatggattt catgttgatg ctcagaaaat tcaatgccgt
     1441 tataatagca cgctcacgca ggcactccga tgcggcctgt aacaaggaag aacaatgaaa
     1501 tcgtggttta atccctctgc aacacaaaac cgtgcgcgtg cgtggcgttt tgccaaaagc
     1561 ctgcgtgatg cccaacttca cccttggaaa gccgaagaag tggctttggc tgagcaattc
     1621 attgccgaaa ccttggatgc agatggctac cgagctgctg tatacgcttt aagtgattgg
     1681 cacaacatca aagaccgcgc cgattttggt ggctatagca aggccgagcg tgattaccgc
     1741 gccgacatca tcgctaccat ggaaatcacc attgctcaaa gtgagaaatg atggccgatt
     1801 tgtggtggca gtggttgggt ttgtggtttt ccgctttcag ctcggcaacc atcttgccag
     1861 gcacgtcgga tgtggtgttg gtggcaatgg tgaatgccca cccacagtgg tgggcttggg
     1921 catggttggt ggcaaccgtg ggcaatggca tgggcggtta tgcctcgtat tggat
//
LOCUS       DAOMVK010000425         1974 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_16887_length_1974_cov_2.806670, whole genome shotgun sequence.
ACCESSION   DAOMVK010000425 DAOMVK010000000
VERSION     DAOMVK010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16887_length_1974_cov_2.806670"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="PK856_11130"
     CDS             complement(<1..805)
                     /locus_tag="PK856_11130"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HRL02961.1"
                     /translation="MLILHQSNRLEHLAEALASLLNAMPLEDLFESETFVVQSQGMRR
                     YINRYLAQKNGIAANIDFSLPARLMWQLMQQNLNNLSKLSPFATDVLHWRLMQLFEEA
                     PFREQPNAAATYLHQYMVSRPEAYFDLAFILADIFDQYLIYRSDWIQHWQNGELLNLG
                     SDEAWQADLWQQLTAQESHHGEHRLQMWQRLQAALPTANLPKRIWVFGITSMAPMYLE
                     LFRTLSQYTDVHIFAMNPSSAYWGNIIEPATLLRQYGHMSEAELAEHNGH"
     gene            1010..1903
                     /locus_tag="PK856_11135"
     CDS             1010..1903
                     /locus_tag="PK856_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HRL02962.1"
                     /translation="MQVISKNAFSLCAIAAALMLSACGGGEKAAEPTAPASGAAASTS
                     STGSGDQVYIIGSDATYAPFEYQDDKGNVIGFSKEVMQAVADKAGFKVQFVNKPWEGI
                     FETLNTGDSDVISSSVTITDERKAQMDFSEPYFEATQMIAVNDAKGGDIKSFEDLKKG
                     KHLISVQTGTTGDIVSQKLLGKDSTQIKRFESMPLALKELLASGVDASVGDNGVVQNF
                     VTNNPDAKLRTIVDPTFEKEYYGFAVKKGRSDDLVKKINTGLAGIKSDGTYDKIYAKW
                     FKSAPSAAAAPAAASAASGAQ"
BASE COUNT          538 a          438 c          520 g          478 t
ORIGIN      
        1 ggtggccatt gtgttcggcc aattctgctt cgctcatgtg cccatattgt ctgagcaagg
       61 tggcgggctc aatgatgttg ccccaatagg ctgagcttgg gttcatggcg aaaatgtgca
      121 catcggtgta ttgcgacaag gtacggaaca attccaaata catcggtgcc atggaggtga
      181 tgccaaacac ccaaatgcgt ttgggcaagt tggcagtcgg caatgcggcc tgtaaacgct
      241 gccacatttg caaacggtgc tcaccgtggt ggctttcttg tgcggtgagc tgttgccaca
      301 aatcggcttg ccacgcttca tctgaaccca aattcagcaa ctcaccgttt tgccaatgtt
      361 gtatccaatc ggaacggtaa atcaaatact ggtcgaaaat gtcggcgagg ataaacgcca
      421 aatcaaaata ggcttcaggc cgactgacca tgtattggtg caaataagtg gctgcggcat
      481 tgggctgttc acgaaacggc gcttcttcaa acaattgcat caagcgccag tgcaacacat
      541 cggtggcaaa cgggctgagt ttggacaagt tgttcagatt ttgctgcatc aattgccaca
      601 tcaagcgggc gggcaggctg aaatcaatgt tggcggcgat gccgtttttt tgcgccaaat
      661 agcggttgat gtagcggcgc atgccttggc tttggacgac gaaggtttcc gactcaaata
      721 agtcttccag cggcatggcg tttaacaaac tggccaaggc ttctgccaag tgttcgaggc
      781 gattggattg gtgcaaaatc agcatgcgca tttccatttg gacaatgcgc cattatgacg
      841 gtgttgtgca tgtgccgcaa tgtgggtaag tcggtcaaag aaggcagtaa atgtcagtat
      901 ttaagttgac caccttgctc gacaaattta tagtgcttgc aaggcagctc aaacttgggc
      961 accaacaaaa tcaaacaacg acaaacaaaa gaaacaattg gagaagaata tgcaagtgat
     1021 ttcaaaaaat gcgttcagtt tatgtgccat tgccgcggct ttgatgttaa gcgcctgtgg
     1081 cggtggtgaa aaagcggctg aacccactgc acctgcatcg ggtgctgccg ccagcacttc
     1141 ttctactggc agtggcgatc aggtgtacat cattggttct gacgcgacct atgcgccgtt
     1201 tgaataccaa gacgacaaag gcaatgtgat aggcttctcg aaagaagtga tgcaagccgt
     1261 ggctgacaaa gctggcttca aagtgcagtt tgtcaacaaa ccatgggaag gcatttttga
     1321 aaccttgaac acgggtgact ctgatgtgat ttcgtctagc gtgaccatca ccgatgagcg
     1381 caaagcccaa atggatttct ctgagcctta ttttgaagcc acgcaaatga ttgcggtgaa
     1441 tgacgccaaa ggtggcgaca tcaaatcgtt tgaagacttg aaaaaaggca aacatttgat
     1501 ttcagtgcaa acgggtacca caggtgacat cgtgtctcaa aagctgttgg gcaaagacag
     1561 cacgcaaatc aagcgttttg aaagcatgcc tttggctttg aaagaattgt tggccagtgg
     1621 tgtagacgcg tctgtcggtg acaacggtgt ggtgcaaaac tttgtgacca acaatcctga
     1681 tgccaaattg cgcaccattg ttgacccaac atttgaaaaa gaatattacg gttttgccgt
     1741 gaaaaaaggc cgcagtgatg atttggtgaa aaaaatcaac acaggtttgg ctggcatcaa
     1801 atcagatggc acttacgaca aaatttacgc caaatggttt aaatcagcac ctagcgcagc
     1861 cgctgcacct gcggcagcat cagccgccag tggcgcgcaa taagcggcct gtaagcgtga
     1921 accacttaag tgttggtatt taagttgctt aaccaagaag tgggtgccaa tagg
//
LOCUS       DAOMVK010000426         1974 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_16888_length_1974_cov_2.534132, whole genome shotgun sequence.
ACCESSION   DAOMVK010000426 DAOMVK010000000
VERSION     DAOMVK010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16888_length_1974_cov_2.534132"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PK856_11140"
     CDS             <1..245
                     /locus_tag="PK856_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HRL02963.1"
                     /translation="LKDEGNQAGIDWVRATPKSSNAGYQYIRIGFKGDQLSRMQLKDS
                     FGNSTTVSFGGLNNSPNLSASSFNFTPPKGVDVLSN"
     gene            complement(315..1799)
                     /locus_tag="PK856_11145"
     CDS             complement(315..1799)
                     /locus_tag="PK856_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02964.1"
                     /translation="MDTKHPDQMYRQAVMNNIQKDNQYNFSGEATFKLQDKTTLDAAT
                     LAMGNVTEPATAAASTPVATSENAGETVDAAAAAVKEAAAAVATDDDEEESHHKRPDW
                     NELYDEMLTEADRYPLVQSFLKNSNIRVEGAVDLPQGKVEMISSLNMATKNYGMWGRL
                     PVQVDAKRESILVDTQGYAGMMESSFFGSNGKIDSIKRLEQGALLEIKQPEDMKQRYP
                     VKSLIRAMPKGLEAYLNAMEAEKFSLQPMDDYGRELRAAYRVQVSYDAIDSIKWSKAL
                     LQGYNQEFLRLQREEPEAGISEKSYNDVKSVLLLGAMMLGGDSNECKKEETADAAADA
                     IVVTDTSSSDTTTTGEFQKELACSSADAFEEMKKEMGQMPKVHEDLYLSRSGRVLGIQ
                     DRMVMLGKKSPQALVYVSRLKMHDYGQPKFTLNAATQPTVSLWQLIKEAQAPEESIEA
                     EQAAADAEAAADTAVFAATEAVAAKPVKAKKAKAKKAKAKKVKS"
BASE COUNT          475 a          516 c          416 g          567 t
ORIGIN      
        1 ccttaaaaga cgaaggcaac caagccggca tcgattgggt gcgtgccacg ccaaaatcct
       61 cgaatgcggg ctaccaatac atccgcatcg gctttaaagg cgaccaattg tctcgcatgc
      121 aattgaaaga cagttttggc aacagcacca ccgtcagctt tggcggcttg aacaacagcc
      181 ccaacttgtc tgcttccagc tttaacttca ccccacccaa aggcgtggat gtgttgagca
      241 attaaatcgt caacatgata aaaaccactg ccatgcagtg gtttttttat ttacaggccg
      301 ctataaagca atccttagga tttgactttc ttagctttgg ccttcttggc ttttgctttt
      361 ttggctttca ctggtttcgc agccacggct tcagttgcag caaaaacggc agtgtctgca
      421 gcggcttcag catcagcagc agcttgctca gcctcaatcg actcttcagg tgcttgtgcc
      481 tctttaatca gctgccaaag cgaaacagta ggttgagtcg cagcatttaa cgtgaatttc
      541 ggttgaccat aatcatgcat cttcaggcga gaaacataca ccaaggcttg tgggcttttc
      601 ttacccaaca tgaccatgcg gtcttgtatg ccaagaacac ggccgctgcg gctcaagtac
      661 aaatcttcat gtactttcgg catttgaccc atctcctttt tcatttcctc aaaagcatcg
      721 gcacttgagc aagccaattc tttttggaat tcaccagtgg ttgtggtatc gctgctgctg
      781 gtatctgtca ccactatggc atcagcggct gcatcggcag tctcttcctt cttgcattca
      841 ttgctgtcac caccaagcat cattgcgccc aacaacaaca ccgatttgac atcgttatac
      901 gatttttcac tgatgcctgc ttctggctct tcacgctgca aacgcaaaaa ctcttggtta
      961 tagccttgca ataaagcctt gctccatttg atactgtcta tcgcatcgta agacacttgc
     1021 acacggtaag ctgcacgcaa ttcacgacca taatcatcca taggctgcaa actgaatttt
     1081 tcggcttcca tggcattcaa atacgcttcc aaaccttttg gcatggcacg aatcaggctt
     1141 ttcactggat agcgttgttt catgtcctca ggctgcttga tttccaacaa agcaccttgt
     1201 tccaaacgtt taatgctgtc tatcttgcca ttgctgccaa aaaagctcga ttccatcatg
     1261 cctgcatagc cttgggtatc caccaagatg ctttcacgtt tggcatccac ttgcacaggt
     1321 aaacggcccc acatgccata atttttggtc gccatattga gcgatgaaat catttccact
     1381 ttgccttgtg gcaaatccac agcgccttca acacggatat tggagttctt taaaaacgat
     1441 tgcaccaaag gatagcggtc agcctcggtc aacatctcat cgtacaattc attccaatca
     1501 ggacgtttgt ggtgcgactc ttcttcatcg tcatcggttg caactgcggc agccgcttct
     1561 tttactgccg ctgccgctgc gtctactgtt tcacctgcat tttcgcttgt cgccacaggg
     1621 gtacttgcag ccgctgtagc aggctcagtt acattgccca tggccaaggt tgctgcgtcc
     1681 aatgtggttt tgtcttgcaa tttgaacgtg gcttcaccac tgaaattgta ttgattgtct
     1741 ttttgaatgt tgttcatgac cgcttggcgg tacatttgat cagggtgctt tgtgtccatg
     1801 gtggtacagg cagacaacac ggccgcggca acacagctaa gcatcagagg gctataacgc
     1861 atacgattct cgagtatttg ttggtaaaca gcccgatatt agcatggaaa aaattaaatg
     1921 ctcaaaaaaa agcggcctgt aagtttacag gacgcttttt atgctactta aatg
//
LOCUS       DAOMVK010000427         1968 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_16977_length_1968_cov_3.584422, whole genome shotgun sequence.
ACCESSION   DAOMVK010000427 DAOMVK010000000
VERSION     DAOMVK010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16977_length_1968_cov_3.584422"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="PK856_11150"
     CDS             <1..544
                     /locus_tag="PK856_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HRL02965.1"
                     /translation="SGEEVTGEHKEIQAADLAAMLKDASSVIITPGYGMAVAQAQYPV
                     AELTQKLRAAGVDVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDV
                     VLVIGANDTVNPAAATDPSSPIAGMPVLHVWDAKNVIVFKRSMSAGYAGVQNPLFFND
                     NSYMYFGDAKDKVSEILQHL"
     gene            673..1410
                     /locus_tag="PK856_11155"
     CDS             673..1410
                     /locus_tag="PK856_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02966.1"
                     /translation="MLEDVKSQSPHIRLFPTAETVFAPDSAFLGQHLLPVVSVDLAAV
                     NPAWSGWLPFVNPIEPYECYIGDGTEAFHNEYAKANVFCLRFNEQGQYEWLADKRYFL
                     LEHHDASDAWQQEMVTELAKERVEAVKQLALTQARYQQYGKFFDNNRHSYSPADFDFA
                     TAEEDVVLDQIGGSVGCGNWEYPLSEYGMVEIIDDETTYMIAPNGKRAYFVAGADGYR
                     FNQRGADWILLFYEPESRLAMFTFDWT"
     gene            1488..>1968
                     /locus_tag="PK856_11160"
     CDS             1488..>1968
                     /locus_tag="PK856_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminodeoxychorismate synthase
                     component I/aminodeoxychorismate lyase"
                     /protein_id="HRL02967.1"
                     /translation="MNQTEYFVVLDDAHSHNSRYYQDFSHIDAIKAVDLHQVDALLKQ
                     GWAQGLHVVLWQSYDFGVELVFGGAATALYLLWFKRCQVLNDTDDTLPWQHAAPVATG
                     IAALHSEVGEAEYLAHIAAIHAAITRGDVYQINYTTAWTGEAYGEPTRLYARLRERQQ
                     "
BASE COUNT          470 a          480 c          557 g          461 t
ORIGIN      
        1 ttctggtgaa gaagtcacag gagaacacaa agaaatccaa gccgctgatt tggctgccat
       61 gctcaaagat gccagcagcg tcatcatcac cccaggctac ggcatggcgg tggcacaggc
      121 acaataccct gtggccgagc tcacgcaaaa actgcgcgcc gcgggtgtgg atgtgcgctt
      181 tggcattcat cccgtggcag ggcgtttgcc tggtcacatg aatgtgttgt tggccgaagc
      241 aaaagtgcct tacgacatcg tgttggaaat ggatgaaatc aacgatgatt tcgccgatac
      301 cgacgtggtg ttggtaatag gggcgaatga cacggtaaac ccagccgctg ccaccgaccc
      361 aagcagcccg attgcaggca tgcctgtgct gcatgtgtgg gatgcgaaga acgtgattgt
      421 gttcaaacgc tcgatgagcg caggttatgc tggcgtgcaa aatccgctgt tcttcaacga
      481 caacagctac atgtattttg gcgatgccaa agacaaggtg tcggagattt tgcaacacct
      541 gtaagctgag tggaaagagt gcaagcggcc tgtaaatgtt tacaggccgc tttttacatg
      601 gctaacaagg cttgaccaaa gcaaaggctt gccgcaatac tgcacacaca ttctcaacca
      661 caaaggttcg ccatgttgga agatgttaaa agccagtcgc cgcacattcg cctgtttccc
      721 actgccgaaa ccgtgtttgc acccgattcg gcatttttag gccaacactt attgcccgtg
      781 gtgagtgtgg atttggcggc ggtgaatccc gcatggtcgg gttggttgcc gtttgtgaat
      841 cccatcgagc cgtatgagtg ttacatcggt gatggcactg aggcgtttca caatgaatac
      901 gccaaggcca atgtgttttg cctgcgcttc aatgagcaag gccaatacga atggctggca
      961 gacaagcgtt atttcttatt ggaacaccac gatgcgagcg atgcttggca gcaagaaatg
     1021 gtgaccgaat tggcgaaaga gcgcgttgag gcggtgaagc aattggcatt gacccaagcg
     1081 cgctatcagc aatacggcaa attttttgac aacaaccgcc attcttacag ccctgccgat
     1141 tttgattttg ccacggccga agaagatgtg gtgttagacc aaatcggtgg cagcgttggc
     1201 tgcggcaatt gggaataccc cttgtctgag tatggcatgg tggaaatcat cgatgatgaa
     1261 accacttaca tgatagcccc gaatggcaag cgcgcttatt ttgtggcggg ggcagatggc
     1321 taccgtttca atcagcgcgg ggccgattgg attttgctgt tttacgagcc agaatcgcgc
     1381 ttggcgatgt tcacctttga ttggacgtaa tcaagcacac aagtgctctc agaatgtgat
     1441 ttaatgcggc ctgtaaatgt gttacaggcc gcttttgtag aagaaacatg aatcagaccg
     1501 aatactttgt cgttttagac gatgcccaca gccataacag ccgttattac caagatttca
     1561 gccatattga tgccatcaag gcggtcgatt tgcaccaagt cgatgccttg ctcaagcaag
     1621 gctgggcgca aggcttgcac gtggtgttgt ggcagtcgta tgactttggg gtggagctgg
     1681 tgtttggtgg cgcagccaca gcgctttatt tgctgtggtt caagcgctgc caggtattaa
     1741 acgacaccga tgataccttg ccgtggcaac acgccgcacc tgtggccaca ggcatcgctg
     1801 ctttgcacag cgaggtcggc gaggccgagt atttggcgca catagcggcc atacacgcgg
     1861 cgattacccg tggcgatgtc taccaaatca actacaccac cgcgtggacg ggcgaggcct
     1921 atggcgaacc cacgcgtttg tacgcgcgtt tgcgcgagcg tcagcaag
//
LOCUS       DAOMVK010000428         1964 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_17016_length_1964_cov_3.486118, whole genome shotgun sequence.
ACCESSION   DAOMVK010000428 DAOMVK010000000
VERSION     DAOMVK010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17016_length_1964_cov_3.486118"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PK856_11165"
     CDS             <1..368
                     /locus_tag="PK856_11165"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRL02968.1"
                     /translation="SLLTAQSYTNQVDNIQRGAGYRLFAVFVPEQKDAIAVCGYRLTH
                     NLVWGRYIYIDDLVTQHTFRRQGYANALMDAVTTEARKLGCTEIHLNANVSPERSAMH
                     SLCYNKDFFIASHHFRRNL"
     gene            391..864
                     /locus_tag="PK856_11170"
     CDS             391..864
                     /locus_tag="PK856_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02969.1"
                     /translation="MPVTREQSYAALASLHPAPIFLQCYQQEELPDMMELFFRPPEVF
                     FISPEIQTQYTHGVLIPILDEGSFDTALLLHPHSGELFCYSIEWPQQVLRSYANWQQY
                     LADLMLRIAETEEDDAEVVRLGKLIGFEDFDALFALLALNGEDYRLTSTQFASQF"
     gene            1423..>1964
                     /locus_tag="PK856_11175"
     CDS             1423..>1964
                     /locus_tag="PK856_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HRL02970.1"
                     /translation="MLTLRKMGFAVATAMLLAACNQGGGAPENKAASAAPAGASGAAT
                     APAAGGELVVKLGTANPLTGAFAEWGKDAANGVKLAVDEANAENLTWNGQKVKFELMS
                     EDDMADPKTAAQVADRFVDAKVSAVIGHLVTGASLPASIVYNDHNIPMVAYSVTGPKF
                     TQQGFKGVFRVISNDKQQGDA"
BASE COUNT          521 a          494 c          428 g          521 t
ORIGIN      
        1 ccagtttgtt gacggcgcaa tcgtatacca accaagtgga caacatccaa cgcggtgcgg
       61 gctaccgttt gtttgccgtg tttgtgcccg agcaaaaaga cgcgattgcc gtgtgtggct
      121 accgcctcac ccacaatttg gtttggggtc gctacatcta cattgatgat ttggtcacgc
      181 aacacacctt ccgccgccaa ggctatgcca atgccttgat ggatgctgtt accaccgaag
      241 ctcgcaaact cggttgcacc gaaatccact tgaatgccaa tgtgtcacca gaacgcagcg
      301 ctatgcacag cctgtgttac aacaaagatt tcttcatcgc cagccaccat ttccgccgca
      361 atctctaagc cgcgaatcag gatgaacaag atgccagtga cccgtgaaca gtcttacgcc
      421 gcactggctt ctttgcatcc agcccccatt tttttgcaat gctatcagca agaagaattg
      481 cccgacatga tggaactctt tttcaggccg cctgaagtgt ttttcatcag ccccgaaatt
      541 caaacccaat acacccacgg tgtgttgatc ccgattttgg acgaaggcag ttttgacacc
      601 gctctgttgc tgcacccgca cagcggtgaa ttgttttgtt acagcataga atggccacaa
      661 caagtcttgc gttcttatgc aaactggcaa cagtatctgg ccgatttaat gctgcgcatt
      721 gccgaaactg aagaagacga tgccgaagtc gtgcgcttgg gcaagctcat tggttttgag
      781 gatttcgatg ccttgtttgc gcttttggcc ttaaatggcg aggattatcg gctgacaagt
      841 acccaatttg cgtcacaatt ttaaaacgcc acaaaatttt tatttagccc gtttaagccc
      901 tggctttcgc aattttaaac ccttagcaaa ggttcatatt ctcaaaattt attgccaatt
      961 attcactcat taatggcaat aaattccaat taaaattcat tcttaacttc tagtaatttg
     1021 atgccacttg tgagcttaca tttctcacaa gacccaatag catagggctt ttaattcaaa
     1081 cccgccattt gaaccgcatc aaaatacagt caaccatctg cacaaaccca agagaaatat
     1141 tctaatatcc caatcaattt tgcaatattg tatattaacc atttatttat aaagaagata
     1201 tgacagaatc gtgtcgaaaa cgcttacagg ccgctactat cgtgcgctaa tctaggtgaa
     1261 tacttgacgc ctcatctaaa aaccgcaaca attcttaacg atatgcacca cagagtgttt
     1321 tcgatttgta ctgttttgca gcttcagccc atacacctca gggtttgctg atgcgaggct
     1381 acaggcacaa ttctcttcat ttggattagc ttaggattga atatgttgac tttacgtaaa
     1441 atgggttttg ccgtagccac agccatgttg ttggccgcgt gtaaccaagg tggcggcgcg
     1501 cctgaaaaca aagcagcttc tgcagcacct gctggtgcca gcggcgcagc cacagcccct
     1561 gccgcaggcg gcgaattggt ggtgaaattg ggtactgcca acccattgac tggcgcgttt
     1621 gccgaatggg gtaaagatgc tgccaatggc gtgaaattgg ccgttgatga agccaatgcc
     1681 gaaaacttaa cttggaatgg tcaaaaagtt aaatttgaat tgatgtcaga agacgacatg
     1741 gccgacccta aaaccgcagc ccaagtggct gaccgttttg tcgatgccaa agtcagcgcc
     1801 gtgattggcc acttggtaac aggtgcgtct ttgccagcgt ctatcgtgta caacgaccac
     1861 aacatcccta tggttgctta ctcagtaact ggcccgaaat tcacccaaca aggctttaaa
     1921 ggcgtgttcc gtgtgatttc taacgacaag caacaaggcg atgc
//
LOCUS       DAOMVK010000429         1963 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_17026_length_1963_cov_2.270964, whole genome shotgun sequence.
ACCESSION   DAOMVK010000429 DAOMVK010000000
VERSION     DAOMVK010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17026_length_1963_cov_2.270964"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PK856_11180"
     CDS             <1..412
                     /locus_tag="PK856_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRL02971.1"
                     /translation="PIIIRANEGYLYQAFDNVLRNAIKYSPQGSVININCSLIKQELL
                     VNITDNGMGVDEAQLPHIFTAFFRADSSGTSNGTGLGLAIAKHVIEQHQGSITAKNVL
                     PHGLQMVFKLPVPKTKSTRQQKHQQAHNSNPTVV"
     gene            complement(493..756)
                     /locus_tag="PK856_11185"
     CDS             complement(493..756)
                     /locus_tag="PK856_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HRL02972.1"
                     /translation="MSELQQRFEQAQKDVVSLAERPQNAILLKLYAYYKQAVDGDVSG
                     EKPGAFDLVAKKKYAAWEEIKGTSSDAAMQLYIDEVQSLLEEE"
     gene            complement(853..1656)
                     /locus_tag="PK856_11190"
     CDS             complement(853..1656)
                     /locus_tag="PK856_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HRL02973.1"
                     /translation="MRIKLITTILFTCVLSACQPQATTENSKNNAASTTNPAKAVASG
                     DMASVKKNIQAALEQAYPEQKVQIKSVDAAPVAGLYEVVVFPNQVVYMDGSGNYMFVG
                     NLLDVKKQINLTEEKMADLNRIDYKALPFDSAIKEVRGNGQYKVAVFSDPDCPFCKKL
                     EHEIAQMTDVTIYTFLMPIDSLHPQATAKSIQLWCQPNRTEAWTKWMRDGVSPAQVPE
                     CANPVAELTKLAEGFGFNGTPTIVYPNGKIQSGYMPKADFEAALKKNQS"
BASE COUNT          520 a          487 c          399 g          557 t
ORIGIN      
        1 acccatcatc attcgtgcca atgaaggcta tttgtatcag gcttttgaca atgttttgcg
       61 caacgccatc aaatacagcc cgcaaggcag cgtgatcaac atcaattgca gcctcatcaa
      121 gcaagaattg ttggtcaaca ttaccgacaa tggcatgggt gtggacgaag cccaattgcc
      181 gcacatcttc accgcttttt tccgtgctga cagcagtggc accagcaacg gcacaggctt
      241 gggtttggcc attgccaagc atgtgataga acaacaccaa ggcagcatta ctgccaaaaa
      301 cgtgttgccg catggtttgc aaatggtatt caaattaccg gttcccaaaa ccaaatccac
      361 ccgccagcaa aaacaccagc aagcacacaa cagcaacccc actgtggttt aaatagagca
      421 caaaaaaagc cgctttctca taagaaagtg gcctgtaaac acatttggcg acattgcgcc
      481 aagtgtgttt ctttattctt cttccaacag actttgcact tcatcaatgt acaactgcat
      541 ggcagcgtcg ctgctggtgc ctttgatttc ttcccaagcc gcatatttct tcttggccac
      601 caaatcaaac gcacccggtt tttcaccgct gacatcgcca tccaccgctt gtttgtagta
      661 agcatacaac tttaacaata tggcattttg agggcgttca gccaaactca ccacgtcttt
      721 ttgagcctgt tcaaagcgtt gttgcaattc actcatggtc ttctctctgt gattcactgc
      781 tgtaacaaag ctctattatt aacacagttt atctggcttg gcagcagatt taatagaaca
      841 attgcccgtt atttacgact ggtttttctt caatgccgct tcaaaatcgg ccttaggcat
      901 gtaacccgat tgtattttgc catttggata cacaatggtc ggcgtgccat tgaaaccaaa
      961 gccctcagct agcttggtca attcagccac agggttggcg cactcaggca cttgggcagg
     1021 gctcacaccg tcacgcatcc acttggtcca tgcttcagtg cggttgggtt gacaccacaa
     1081 ttgaatggac ttggccgtgg cttgtgggtg caatgagtca attggcatca agaaagtgta
     1141 gatggtgaca tcggtcattt gcgcgatttc atgttccaat tttttacaga atgggcaatc
     1201 tggatcgctg aacaccgcca ctttgtattg gccattgcca cgcacttctt tgatggccga
     1261 atcaaatggc agagccttgt agtctatgcg atttaaatcc gccatttttt cttcggttaa
     1321 attgatttgc ttcttcacat ccaacaaatt gcccacaaac atgtaattac cactaccatc
     1381 catgtaaacc acttggttcg gaaacaccac tacctcatac aagcccgcaa caggtgccgc
     1441 atccacactc ttgatttgta ctttttgctc aggataagcc tgctccaatg cggcctgtat
     1501 gttctttttc acactcgcca tatcgccact ggcaacagct ttagctgggt ttgttgtaga
     1561 agcggcattg ttcttgctgt tttccgttgt ggcttgaggc tgacaagcac tcaacacaca
     1621 agtaaagagg atggtggtaa ttaatttaat ccgcataaaa ttctcttaaa attgattgct
     1681 aaaaattaca aatcttggca atattgtcaa aatgtcaaat ggcatattgt tttaccttgt
     1741 aatgattaag tttttccacc agcatcgtaa catatccaaa gatttaagtg gttcattgtt
     1801 cttcaatcac tcgtacggag gctctatggc tcaaactcgt ttggtagcaa ctggtattac
     1861 atcaaaaaca gacgccgaca aaattgcgca agcaggcgaa gcagttgaag gtgttcgctt
     1921 cgtcaatgtc aatcatgatg acggtggtat tgtcatcacc cac
//
LOCUS       DAOMVK010000430         1952 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_17145_length_1952_cov_2.983658, whole genome shotgun sequence.
ACCESSION   DAOMVK010000430 DAOMVK010000000
VERSION     DAOMVK010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17145_length_1952_cov_2.983658"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /gene="eno"
                     /locus_tag="PK856_11195"
     CDS             <1..440
                     /gene="eno"
                     /locus_tag="PK856_11195"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRL02974.1"
                     /translation="IEDGMSEHDWAGWKILTDKLGDKIQLVGDDLFVTNPAILAEGIE
                     QGLANSLLVKVNQIGTLTETLKAVDLAKRNRYTSVMSHRSGETEDNTIADLAVATNCM
                     QIKTGSLSRSDRMSKYNQLLRIEEELGAAAYYPGRAAFYQLAK"
     assembly_gap    519..528
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <529..740
                     /locus_tag="PK856_11200"
     CDS             <529..740
                     /locus_tag="PK856_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003743241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HRL02975.1"
                     /translation="RLQKQLASNEDATQRNDALKAELGDLNSGGDAIGELARYELGYV
                     GVGETYHRIVPKVTTEAPPPVTVIE"
     gene            843..1670
                     /locus_tag="PK856_11205"
     CDS             843..1670
                     /locus_tag="PK856_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRL02976.1"
                     /translation="MIVAAIFFAIMGALVKLGADRFGSVELVFYRTVFSVVVLASLAI
                     ARGDSLKTPYLKQHISRGIAGTLGILAFFYALTRLPLATAVTLNYTSPIFLALLSFVL
                     LKERISRRTVAILLLGFVGVMILLRPSIGGDQFVAGLVGLSAGVFAGWAYLQVRELAL
                     LGEAEWRVVLYFSTVAALIAAIIVSVQGWHVPSSWQDVLILGGVGLTALIAQMSMTRA
                     YKVGRKFVVASFSYLTVVFSTLLGVWWFGEVLQWQELAGIAVIVLSGILGSVLPAKK"
     gene            1698..>1952
                     /locus_tag="PK856_11210"
     CDS             1698..>1952
                     /locus_tag="PK856_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HRL02977.1"
                     /translation="MISIRQQSYGLNVALFNEFTVEDFKLLEQSVTDVIQEHDHPDLM
                     LDLSLMRDFTIDMAWEQVRFARQHMGEFGLVAIVVNDWWIE"
BASE COUNT          435 a          411 c          572 g          524 t
ORIGIN      
        1 cgattgaaga cggcatgagc gagcacgact gggcaggttg gaaaatcttg accgataaat
       61 tgggcgacaa aatccaattg gtgggcgatg atttgtttgt gaccaatcct gcgattttgg
      121 ccgaaggcat agagcaaggc ttggccaact cattgttggt gaaagtaaac caaatcggta
      181 ccttgaccga aaccttgaaa gccgtcgatt tggccaaacg caaccgttac acttctgtga
      241 tgagccaccg ttcaggcgaa accgaagaca acaccattgc cgatttggct gtggcgacta
      301 actgcatgca aatcaaaacc ggctctttgt cgcgttctga ccgcatgtct aaatacaacc
      361 aattgttgcg cattgaagaa gaattgggcg cagcggctta ctatccaggc cgcgcggcat
      421 tctatcaatt ggcaaaataa cacggctgaa acaaaatgaa gtgggtcaca ctggtattat
      481 tgggcgcttt gacttatgtg cacttccact tgtggttgnn nnnnnnnnag cgtttgcaaa
      541 agcaattggc cagtaatgaa gatgccactc aacgcaacga tgccttgaaa gcggagctgg
      601 gggatttgaa cagcggtggt gatgccatag gtgagttggc aaggtatgag ttgggctatg
      661 ttggcgtggg ggaaacctat caccgcatcg tgcccaaagt caccaccgaa gccccgcctc
      721 ccgtgaccgt tatcgagtag tgtgaccgaa ttaaaacagc tttcaggccg cctgaaagct
      781 gtttttgtct gtgcgacaac aaaaaaggag aaaaagtgat acagcgcttg ggttctggtt
      841 ggatgatagt tgcggccatt ttttttgcca tcatgggggc tttggtcaag ctgggggcag
      901 accgttttgg ttcggtagaa ttggtgtttt atcgaacggt gttttcggtg gtggtgttgg
      961 caagtctggc gattgctcgc ggtgacagcc tgaaaacgcc gtatttgaag caacacattt
     1021 cgcgtggcat tgctggcact ttgggcatat tggcgttttt ctatgcgctc acgcgtttgc
     1081 ctttggccac tgccgtgacc ttaaactaca cttcgccgat tttcttggca ttgctgtcgt
     1141 ttgtgctatt gaaagaacgc atcagccgcc gcacggtggc gattttgttg ctgggctttg
     1201 tgggtgtgat gatattgctg cgcccgagca taggcggtga ccaatttgtg gcaggcttgg
     1261 tgggcttgag tgcgggcgtg tttgctggct gggcgtattt gcaagtgcgt gaattggcct
     1321 tgctgggcga ggccgagtgg cgggtggtat tgtatttctc tacagtggcg gccttgattg
     1381 cggccatcat cgtcagcgtg caaggttggc atgtacccag ttcttggcaa gatgtgttga
     1441 ttttgggtgg cgtgggctta accgcgctga ttgcccaaat gagcatgacc cgcgcttaca
     1501 aagtggggcg caaatttgtg gtggcttcat tttcgtattt gacggtggtg ttttccacct
     1561 tactgggggt atggtggttt ggtgaggtgt tacaatggca agagttggct ggcatcgcgg
     1621 tcatcgtgct cagtggtata ctgggaagcg tgttacccgc caaaaaataa atctttgaca
     1681 cccaaaagga ggacgcaatg atatccatca gacaacaatc ctacggcctg aatgtggcct
     1741 tgttcaatga gttcactgtc gaagatttca aattgctaga gcaaagtgtg accgatgtca
     1801 ttcaagagca tgaccatcct gatttgatgc tggatttgtc gctgatgcgc gatttcacca
     1861 tagacatggc ctgggagcaa gtgcgttttg cccgtcagca catgggtgaa tttggcttgg
     1921 tggccatcgt ggtaaatgat tggtggatag ag
//
LOCUS       DAOMVK010000431         1934 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_17332_length_1934_cov_2.474188, whole genome shotgun sequence.
ACCESSION   DAOMVK010000431 DAOMVK010000000
VERSION     DAOMVK010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17332_length_1934_cov_2.474188"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /gene="pncB"
                     /locus_tag="PK856_11215"
     CDS             complement(<1..1042)
                     /gene="pncB"
                     /locus_tag="PK856_11215"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019358 - nicotinate nucleotide salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HRL02978.1"
                     /translation="MNPRPVIIQSLLDTDLYKFTMLQTVLHQFPQTHSVYHFRCRNLE
                     KIAYPLVDIKAELDEELDILCELKFKADELDYLRSLRFIKSDFVDYLELFQLKRRFIK
                     TTVDAEGRLDIVVEGPMIQAMFFEIFVLAIVNELYFRRLETPEVLAEGERRLQAKAAL
                     LRDYDAQQGNDEPPFVVSDFGTRRRYSYQWQRHVIETLHAAAPRIIRGTSNVYIAKEL
                     GITPIGTMAHEFMQAFQALNVRLRDFQKAALESWVHEYRGDLGIALTDVVGMDAFLND
                     FDLYFAKLFDGLRHDSGDPYEWGDKAYVHYQKLRINSQTKMLTFSDGLDLQKSWDLHQ
                     YFKDRFKTSFGIG"
     assembly_gap    1250..1259
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1339..>1934
                     /locus_tag="PK856_11220"
     CDS             1339..>1934
                     /locus_tag="PK856_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HRL02979.1"
                     /translation="MKSPELLLPAGGLERMRTAFDYGADAVYAGQPRYSLRARNNEFA
                     KLSELQTGIEEAHARGKKFFLASNILPHNSKLKTYLDDMAPIMEMNPDALIMADPGLI
                     MKVKERWPEAVIHLSVQANTTNYWGVQFWEKIGVERIILSRELSMDEIAEIRQQCPDM
                     ELEVFVHGALCIAYSGRCLLSGYFNHRDPNQGTCTNACR"
BASE COUNT          494 a          453 c          483 g          494 t
ORIGIN      
        1 tgcctatgcc aaaactggtt ttgaagcggt ctttgaaata ctggtgcaaa tcccaagact
       61 tttgcaagtc caagccatcg ctgaatgtca gcattttggt ttgggaattg atgcgcagtt
      121 tttggtaatg cacataggct ttatcgcccc attcgtaagg gtcgcccgaa tcgtggcgca
      181 aaccgtcaaa caatttggca aaatacaaat cgaagtcatt taaaaacgca tccatgccca
      241 ccacatcggt gagggcaata cccaagtccc cacggtattc gtgtacccag ctttccaaag
      301 cggctttttg aaagtcacgc aaacgcacgt tcaaggcttg gaaagcctgc atgaattcgt
      361 gtgccattgt gccgatgggg gtaatgccca attctttggc gatgtacaca ttggaggtac
      421 cacggatgat gcgcggcgca gcggcatgca atgtttcgat gacgtggcgt tgccattggt
      481 agctgtagcg acggcgtgtg ccaaagtcag acaccacaaa tggcggctcg tcattacctt
      541 gttgggcatc gtagtcgcgc agcaaagccg cttttgcttg caagcggcgt tcgccttctg
      601 ccaagacttc gggtgtttcc aagcggcgga aatacaattc gttgacgatg gcgagcacga
      661 aaatctcgaa gaacatcgct tgtatcatcg ggccttcaac gactatgtcc aagcggcctt
      721 cagcatccac tgtggttttg atgaagcggc gtttgagttg gaacagctct aaataatcaa
      781 caaaatcgct tttgatgaaa cgcaacgagc gcaaataatc cagttcatcg gctttgaatt
      841 tcaattcgca caagatgtcc agctcttcat ccaattccgc tttgatgtcg accaaagggt
      901 aggcgatttt ttccaaattg cggcagcgaa aatggtaaac actgtgggtt tgcggaaatt
      961 ggtgcaacac cgtttgcaac atggtgaatt tgtacaaatc ggtgtctaac aaagattgga
     1021 tgattacagg acgtggattc atggtttcac actgtcgtga cgtattaaag tatgcacatt
     1081 aaagcatggt ttgcaggctt tgtgtccgtc aaatcacgca aatgccaatt cgttgtggtg
     1141 agcttgggta tgcttgtgca gtatattgcg ggtttatgtg tttgaatgga tgctgaaaac
     1201 gccgatttga atgcggcctg aaattgaccg aaagcccaaa aaagcccatn nnnnnnnnng
     1261 cagtgccctt tggggcttta attatttgtg cgctgagcat gtcgcattgg cgccgacaca
     1321 ttatcaagac acatacccat gaaatcaccc gaattactgt tgcccgctgg cggtttggag
     1381 cgcatgcgca ccgcgtttga ttatggcgcc gatgcagtgt atgccggcca gccacgctac
     1441 agcttgcgcg cgcgcaacaa tgagtttgcc aaattatctg agttgcaaac tggcatagaa
     1501 gaagcacatg cacgtggtaa aaaattcttc ttggccagca acatcttgcc acacaattcc
     1561 aaattgaaaa cgtatttgga cgacatggca cccatcatgg aaatgaatcc agacgcgctt
     1621 atcatggccg atcctggtct catcatgaag gtcaaagagc gttggccaga ggcagtgatt
     1681 cacttgtctg tgcaagcgaa caccaccaat tactggggcg tgcaattctg ggaaaaaatt
     1741 ggcgtagagc gcatcatttt gtcgcgcgaa ttgtctatgg acgaaatcgc tgaaatccgc
     1801 caacagtgcc cagacatgga attggaagtg tttgtacacg gcgctttgtg cattgcttat
     1861 tcaggccgct gcttgttgtc tggttatttc aaccaccgcg acccaaacca aggcacttgc
     1921 accaatgctt gccg
//
LOCUS       DAOMVK010000432         1933 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_17350_length_1933_cov_2.798722, whole genome shotgun sequence.
ACCESSION   DAOMVK010000432 DAOMVK010000000
VERSION     DAOMVK010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17350_length_1933_cov_2.798722"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..893
                     /locus_tag="PK856_11225"
     CDS             30..893
                     /locus_tag="PK856_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRL02980.1"
                     /translation="MAKLKGLGRGLDSLLASGDAGGDDKLTTLDVAHIRPGRYQPRRV
                     MDEAELIELADSIRAQGLIQPVVVRELGLSEYELIAGERRWRACQLAGLTEIPAVIKA
                     VNDEAALAMGIIENIQRADLNPVEEAQGYKRLVDEFGLTHENLAQAVGKSRSAISNSL
                     RLLSLPEQIQQYINQGLLEMGHARALITLPVLGQLQLAEVAIKQAWSVREMERQAQAW
                     QQNQEPASKKAVSAVDSDVLRLQDAIADKLGLAVKIQANQQQKGKLVLHFNNADEFQQ
                     MLNALNLDQLA"
     gene            1055..1405
                     /locus_tag="PK856_11230"
     CDS             1055..1405
                     /locus_tag="PK856_11230"
                     /inference="COORDINATES: protein motif:HMM:NF015834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HRL02981.1"
                     /translation="MQKIISYQLLVVALFGLILYLFWGQKAAVSGVVGGLCYAVPSIF
                     AVLILSLSSRSPLLSYAGFMMAEGLKIALAAMMLLLIMYFFHSQIMWLPFIIGLFLAS
                     HIVFIVFWKLNHGE"
     gene            1395..>1933
                     /locus_tag="PK856_11235"
     CDS             1395..>1933
                     /locus_tag="PK856_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FoF1 ATP synthase subunit a"
                     /protein_id="HRL02982.1"
                     /translation="MASESSGNWAAEYINHHLVNYTNQPEAQKNIVDFSYWNIDSIVV
                     SVLLGALVLFVLWRGAKTATAGVPTRFQAACELMIEFVDDMCKSVIDNAESRKVIAPL
                     GLTLFVWIFVMNAMDLLPVDLLPVIWQAIQGDHHAYLRAVPTADLNIPLALAIGVLML
                     CIYYSIKIKGFGGWMHEMFS"
BASE COUNT          479 a          366 c          518 g          570 t
ORIGIN      
        1 caagaattat tggcgagggc aggggcgtaa tggcgaaatt aaaaggtttg ggacgcggtt
       61 tggattcgtt gttggccagc ggtgacgcag gcggtgacga taaattgacc actttggatg
      121 tagctcacat tcgaccaggg cgctatcaac ctcgccgtgt catggatgag gctgaattga
      181 ttgaattggc cgactccatc cgagcccaag gcttgataca gcctgtggtg gtgcgcgaac
      241 tcggtttgtc agaatatgaa ttgattgcgg gtgagcgccg ttggcgagca tgtcaattgg
      301 cgggcttaac agaaattcct gctgtcatca aagcagtgaa tgatgaggcc gctttggcga
      361 tgggtatcat tgaaaacatt cagcgtgcgg atttgaatcc agtagaagag gcgcaaggtt
      421 acaagcgctt ggtggatgaa tttggcctaa ctcatgaaaa tttggctcaa gccgtgggca
      481 aaagccgcag tgccatcagc aacagtttgc gtttgttgag cttgcctgag caaattcagc
      541 aatacattaa ccaaggcttg ttggaaatgg gtcatgcgcg cgctttaatt actttgcctg
      601 tgttggggca gctgcaactg gcggaagtcg ccattaagca agcttggtca gtgcgagaga
      661 tggagcgaca agctcaggca tggcagcaaa accaagagcc agccagtaaa aaggcagtca
      721 gtgctgtgga tagcgatgtt ttgcgcttac aggacgctat tgccgataag cttgggttgg
      781 cagtgaaaat acaagccaat cagcaacaaa agggcaagtt ggtgttgcat tttaataatg
      841 cagatgaatt tcaacaaatg ttaaacgcat taaatttgga tcaattggcc taaatcgttt
      901 acatctgttg tttttttgtg tcgcagcgca atgtatgggc aagctgttaa aaatacaatt
      961 ctttttgcgt attgtttgac aaaattttac tgattatctt gactgtacaa agcgctttca
     1021 ttatagtacg ggggctttta aaggccagct cagcatgcag aaaataattt cgtatcaact
     1081 cttggttgtg gctttgtttg gcctgatttt atacctcttt tgggggcaga aagcggcagt
     1141 gtcaggtgtg gtgggtggtt tgtgttatgc ggttccctcg atattcgcgg tgttaatttt
     1201 aagcctttct tctcgaagtc cattattgtc atatgcaggt tttatgatgg cagaaggttt
     1261 aaaaatagca ctggcagcaa tgatgttgtt gttgattatg tatttcttcc acagccaaat
     1321 catgtggttg ccattcataa tcggcttgtt tttagccagt cacattgttt ttattgtttt
     1381 ttggaaatta aatcatggcg agtgaaagca gcggcaattg ggccgccgag tatatcaatc
     1441 accacttggt gaactacaca aaccaacctg aagcgcagaa aaacatcgtt gatttctcgt
     1501 actggaatat agacagtatc gtggtatcag tgttgttggg tgcattggtg ttgtttgtat
     1561 tgtggcgcgg tgccaaaact gctacggcag gcgtaccaac ccgttttcag gccgcttgtg
     1621 agttgatgat tgagttcgtt gacgacatgt gcaaatcggt tattgataat gcagaatctc
     1681 gcaaagtgat tgccccattg ggtttgacct tgtttgtgtg gatttttgtc atgaacgcga
     1741 tggacttgtt gccagtggac ttgttgccag tgatttggca agccatccaa ggtgatcacc
     1801 acgcatactt gcgtgccgtg cctaccgcag acttgaacat cccattggct ttggccatcg
     1861 gcgtgttgat gttatgtatt tactacagca tcaaaatcaa aggttttggt ggttggatgc
     1921 acgaaatgtt ctc
//
LOCUS       DAOMVK010000433         1932 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_17365_length_1932_cov_2.146510, whole genome shotgun sequence.
ACCESSION   DAOMVK010000433 DAOMVK010000000
VERSION     DAOMVK010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17365_length_1932_cov_2.146510"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(226..1041)
                     /locus_tag="PK856_11240"
     CDS             complement(226..1041)
                     /locus_tag="PK856_11240"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRL02983.1"
                     /translation="MTVNSPARPISYSRRLKRLWLSWLWLVHLSRAARRSAKNKDISH
                     TAKLMQQVSQQLLDALNVRVELHGKIPEDLNGLLVVANHTSWLDILAMASVHPMQFIA
                     KQEIKSWPVLGKIVVAMGTLFINRAQRKDTAKINAMITEELHLGGTVAFFPEARTSDG
                     KGLRAFKGALFQAAIDSNSAVLPIALRYFNADGKRSSHAAFTDEVSLPKSFFIILSEE
                     ETVVRVDFTDVLRPETRTEDTSRFTLKQQAQQQVASLVLDDRDLQKLSEWDYL"
     gene            complement(1056..1877)
                     /gene="mutM"
                     /locus_tag="PK856_11245"
     CDS             complement(1056..1877)
                     /gene="mutM"
                     /locus_tag="PK856_11245"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HRL02984.1"
                     /translation="MPELPEVETTRRGIAPYIEGQTIDHVVVRQSKLRWPIPDDLNQQ
                     TAACKITAVARRAKYLLIHLPHGVIMVHLGMSGSLRVFEADAVPEAGKHDHVDLVLQN
                     GTVLRYHDPRRFGAWLWFSGAVETHQLIEKLGPEPLEDDFTADYLQAALSTQKRALKL
                     ALMDNHIVVGVGNIYANETLFRCKLLPTTVARDVTLEQCQALVNEIKAVLTLAIAAGG
                     STLRDFVNSDGKSGYFQQQYFVYGRDGEACHECGEPIAKAVIGQRSSFYCPHCQH"
BASE COUNT          451 a          478 c          517 g          486 t
ORIGIN      
        1 caaacaaatc gtcggtggtg atggttttcc aagcatcgcc ttcgcgaact gggaatgata
       61 cttgaggaat gcgttgacct tctttgctag ctaatgccat tttgtaatct ccattgaata
      121 aagtaaatgt cagtgcgggt ggcactgcgc tgtgattcga tgaagggcat tatgggctgc
      181 tggcttgatt aagtgaattt atcaatttct attaattgat taaatttata aataatccca
      241 ttccgagagt ttttgtaaat cgcggtcgtc caacaccaag ctcgccactt gctgctgtgc
      301 ttgctgtttt aaggtaaagc gcgaggtgtc ttcggtgcgg gtttcaggtc gcaagacatc
      361 ggtaaagtcc acgcgcacca cggtttcttc ttcgcttaaa atgatgaaaa acgatttggg
      421 caggctcact tcatcggtaa atgccgcgtg gctggagcgc ttgccatctg cattgaaata
      481 gcgcaaggca atgggcaaca ccgcgctgtt gctgtctatc gcggcttgaa acaaagcgcc
      541 tttaaacgcg cgcaagcctt tgccgtcgga agtgcgcgct tcggggaaaa acgccaccgt
      601 gccaccaaga tgcaattctt cggtaatcat ggcattgatt ttggccgtgt ctttgcgttg
      661 ggcgcggttg atgaacaaag tgcccatggc cacgacaatt ttgcccaata caggccacga
      721 tttgatttct tgcttggcga taaactgcat tgggtgcacc gaggccatgg ccaagatgtc
      781 cagccacgac gtgtgattcg ccaccaccaa caagccattc aaatcttcgg ggattttgcc
      841 gtgcaattct acccgcacat tcaaggcatc gagcagctgt tgcgacactt gctgcatgag
      901 cttggcggtg tggctgatgt ctttgttttt ggcgctgcga cgggctgcgc gtgacaaatg
      961 caccaaccac agccacgaca gccacaagcg tttgaggcgg cgtgaatatg aaatggggcg
     1021 cgcgggtgaa ttaacagtca taaaaattca aatactcaat gttggcaatg ggggcaataa
     1081 aaacttgagc gctggccgat aacggctttg gcaatcggct cgccacattc atggcaggct
     1141 tcgccatcgc gcccgtacac gaagtattgt tgctgaaaat agccactttt gccatcgctg
     1201 ttcacaaagt cgcgcaaagt gctgccacca gcggcaatgg ccagtgtcaa tacggctttg
     1261 atttcattca ccaaagcttg gcattgttcc aaagtcacgt cgcgggccac ggtggtgggc
     1321 aagagtttgc aacggaaaag ggtttcgttg gcgtaaatat tgcccacacc gaccacaatg
     1381 tgattgtcca tcaaggccag tttcaacgcg cgcttttgtg tgctcaatgc ggcctgtaaa
     1441 tagtcggcgg tgaaatcgtc ctctagaggc tcaggcccca gtttttcgat cagttggtgg
     1501 gtttctaccg caccgctgaa ccacaaccaa gcaccaaaac ggcgtgggtc gtggtagcgc
     1561 aatactgtgc cattttgcaa caccaaatcc acatggtcgt gtttgcccgc ttcgggcacg
     1621 gcatcggctt caaacacgcg caaactgccc gacatgccca aatgcaccat gatgacgcca
     1681 tgcggcaggt gaatgagcaa gtatttggcg cggcgcgcca cggcagtgat tttacaggcc
     1741 gctgtctgtt ggtttaagtc atcgggaatc ggccagcgca atttgctttg gcgcaccacc
     1801 acatggtcta tggtttgacc ttctatataa ggggcgatgc cacggcgggt ggtttcgact
     1861 tcgggtaatt caggcatgtg cgggctttct tgtcagactc aggcctagga aataaggctg
     1921 aaaacttgtg at
//
LOCUS       DAOMVK010000434         1927 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_17417_length_1927_cov_3.494124, whole genome shotgun sequence.
ACCESSION   DAOMVK010000434 DAOMVK010000000
VERSION     DAOMVK010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17417_length_1927_cov_3.494124"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1927
                     /locus_tag="PK856_11250"
     CDS             <1..>1927
                     /locus_tag="PK856_11250"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HRL02985.1"
                     /translation="PNLYHVRAAKQFGQAGENVRFAYNLTSGNGEQKPVRYRVVRLED
                     QSDFDGKLGSDNSIDIAFKQSGSYSIELLDKNDNLIGATNYWVQGKDLQAAPLSVEIV
                     PDKTQYNIGDTAQVLLTFPVPVQDALLTLERENVAQHGLLSQAGSWMQVKKLSDKQYQ
                     LAIPVTENMAPNMVLSVLYQHDNQYAFENAGLVVAKPQLNINVEADQTTYKPKQQVSL
                     DISTLFEGEGGVPADLTVSVVDEMVYVLQPEIAPSMGEFFNHLRRNQVTTESSLNFIT
                     YDQSVSAKGTPESSSMAPRERAVKVLERPRRDDQDTALWQPKLQTDASGHSKLTFTLP
                     DALTRWRITVRAQDSQGRVGQRVAHITSSQPYYLKWSSPNVWRTGDQMSADVLVFNQT
                     EQASTMQWQALIDNQVVKQGDLEVKAGANYLRLPTEGLNGVVTLQLLQNGQVVDSLNQ
                     RVSSVAATWATPQQQTLSLQEAQSGLKLPADARNIRASVLGDGDLAFNRALDGLVDYP
                     YGCVEQTASRLIPLNLALKRLPATTPKWIQASLSERLSNQRLRLIDMAGEGAQFSWWG
                     NQTDGDLLMSSYAYYADFLSGQTLGLSRNPDDANVLLELYKKQAENTPITEVMLSLWL
                     MREMGLPVNNLASGANRR"
BASE COUNT          491 a          567 c          514 g          355 t
ORIGIN      
        1 gccccaatct ctaccatgtg cgcgccgcaa aacaatttgg gcaagcgggt gaaaacgtgc
       61 gctttgccta caatttgacc agtggcaatg gcgaacaaaa gcctgtgcgc taccgcgtgg
      121 tgcgtttgga agaccaaagc gatttcgatg gcaaactggg cagcgacaac agtattgaca
      181 ttgccttcaa acaaagcggc tcttacagca tcgagctttt ggacaaaaac gacaacctga
      241 ttggtgccac caattattgg gtgcaaggca aggatttaca ggccgctcct ttgagcgtgg
      301 aaatcgtgcc cgacaaaacc caatacaaca tcggcgacac cgcccaagtt ttgctcacct
      361 tccctgtgcc cgtgcaagat gccttgctca cactggagcg tgaaaacgtg gcccaacacg
      421 gcttgctctc acaagcgggc agctggatgc aggtgaaaaa actgtcggac aagcaatacc
      481 aattggcgat acctgtaacc gaaaacatgg cgcccaatat ggtgctgtcg gtgctctacc
      541 aacacgacaa ccaatacgcg tttgaaaacg caggcttggt ggtggccaaa ccccaactca
      601 acatcaacgt tgaggccgac caaaccactt acaaacccaa gcagcaagtc agcctcgaca
      661 tttcaacctt gtttgagggc gaaggcggcg tgcctgcgga tttgaccgtg agcgtggtcg
      721 atgaaatggt gtatgtgttg cagcctgaaa tcgcaccgag catgggcgaa tttttcaacc
      781 atttgcgccg caaccaagtc accaccgaat ccagcctcaa cttcatcact tacgaccaat
      841 cggtcagcgc caaaggcaca cctgaaagca gcagcatggc gccgcgagaa cgggcggtga
      901 aagtcttgga gcggccgcgc cgcgacgacc aagacaccgc cttgtggcaa cccaagctcc
      961 aaaccgatgc cagcggccac agcaaactca cctttaccct gcccgatgcg ctcacgcgct
     1021 ggcgcatcac cgtgcgcgcc caagacagcc aaggccgcgt tggtcaacgc gtggcccaca
     1081 tcacctccag tcagccgtat tacctgaaat ggagctcgcc caatgtgtgg cgcacgggcg
     1141 accaaatgag cgccgatgtg ttggtgttca accaaaccga acaagcctcg accatgcagt
     1201 ggcaagcgct catagacaac caagtcgtga aacaaggcga tttggaagtg aaggcgggcg
     1261 ccaattattt gcgcttgccc accgaaggtt tgaatggcgt ggtgacactg caattgctgc
     1321 aaaacggcca agtggtcgac agcctcaacc aacgcgtcag cagtgtggcc gccacttggg
     1381 caacgccaca acagcaaacc ctgagcctac aagaagccca aagcggcctg aaactgcccg
     1441 ccgatgcgcg caacatccgc gccagtgtgt tgggtgatgg cgatttggcg tttaaccgcg
     1501 cactcgacgg cttggtggat tatccgtatg gctgcgtgga gcaaaccgcc agccgcttga
     1561 taccactgaa tctcgccctc aaacgcttgc ccgcgaccac gcccaaatgg atacaggcca
     1621 gcttgagcga gcgcctgagc aatcagcgtt tgcgcctcat cgacatggca ggcgaaggcg
     1681 cgcaattctc atggtggggc aaccaaaccg atggcgattt gctgatgagc agctatgcgt
     1741 attacgccga cttcctcagc ggccaaacct tgggcttgag ccgcaatcca gacgatgcca
     1801 atgtgttgct ggagttgtat aaaaaacagg cagaaaacac tcccatcacc gaagtgatgc
     1861 tcagtctgtg gctgatgcgc gaaatgggtt tgcctgtgaa caatttggcc agcggtgcca
     1921 accgccg
//
LOCUS       DAOMVK010000435         1925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_17439_length_1925_cov_2.373262, whole genome shotgun sequence.
ACCESSION   DAOMVK010000435 DAOMVK010000000
VERSION     DAOMVK010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17439_length_1925_cov_2.373262"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="PK856_11255"
     CDS             complement(<1..723)
                     /locus_tag="PK856_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRL02986.1"
                     /translation="MQLNQIIRADILAMTAYKVTEVPADCIKLDAMESPYEYAEDLKA
                     ELAKELANTPLRLYPTPYARGLPDAIRALHKIDAGADILLGTGSDELIRLLTMLTGQA
                     GSTMLALEPSFVMYRVNAEFFGMKYVGVPLNEDFTMNVEAVLAAIAEHQPQLIFIAYP
                     NNPTGVPFSVDDVNRIIAAAPGLVVIDEAYGAFSSHTFLQQAGTMENVVVLRTLSKIG
                     FAGIRVGYAVGHPSVMHELRKIT"
     gene            1028..1474
                     /locus_tag="PK856_11260"
     CDS             1028..1474
                     /locus_tag="PK856_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02987.1"
                     /translation="MTSRAIWLWTLVWMTLTTGFFISKHSGLPVFTSASALMQMAASH
                     HSPSDRSTVQSDTHADAATNSHSSSHQQNCQTGSNQSCHDTAHQSNDCLWQCLLVCLT
                     QMLLFVMCLAIGLKIRLLAYRKLALSFKSSLLKRLLKPPKNLTVFA"
     gene            1505..>1925
                     /locus_tag="PK856_11265"
     CDS             1505..>1925
                     /locus_tag="PK856_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bilirubin oxidase"
                     /protein_id="HRL02988.1"
                     /translation="MINRRRFLSYGLGVGAAGLAFACNKSAANSDAHSMHGMQNASDN
                     TTSHQNMDHSGHQMGGMNHAANEQDLLPVSALSTGAALAALPLLANESKQAGKFTGRL
                     IAKKHSLKLAKGLESEFWLYNGVLGGPQIVVYEGDEVS"
BASE COUNT          480 a          514 c          430 g          501 t
ORIGIN      
        1 ggtgattttt ctcaattcgt gcatcacgct aggatgccct accgcatagc ccacacgtat
       61 gccggcaaag ccgattttac tcaaggtgcg caacaccacc acattttcca tcgtacccgc
      121 ttgctgtaaa aaggtgtgcg atgaaaatgc accataggct tcatctatca ccaccaaacc
      181 aggtgcggcg gcaatgatgc ggttcacatc atctactgag aatggtacgc ccgttggatt
      241 attgggatag gcaatgaaaa tcaactgtgg ctgatgttcg gcaatcgctg ctaatactgc
      301 ctctacattc atggtgaaat cttcgttcaa aggtacgccc acgtatttca tgccaaaaaa
      361 ttcggcattg acgcggtaca tcacaaacga aggctcaagc gccagcatgg tgctgcccgc
      421 ctgccctgtc aacatggtca acaagcgaat caattcgtct gaaccagtgc ccaataaaat
      481 atccgcgcct gcatcgattt tgtgcaaagc gcggatggca tcgggcagac cacgggcata
      541 cggtgtcgga tacaatcgca aaggcgtgtt ggccagctct tttgccaact cggcctttaa
      601 atcctcagca tattcataag gactttccat cgcatcaagc ttgatgcaat cggctggcac
      661 ttcggtcact ttataggccg tcatggccaa gatgtctgct cggatgattt ggttcagctg
      721 catggtttcg cctttgtttg tgttttggtt ttgttcatga ttgaaacagt gccagtcatg
      781 ctggttttca ctctagcata gtttgtctca atcgggtttg cacatcaggc aaacttttta
      841 tgctgtattg cagcattttc tgcttcaaaa ccaatctatt tagaggataa atacctggat
      901 acaaaccctt tttaacacca ctccttgcgc cccaagctgg acgctaaaaa ccagcctgtg
      961 ctatgcttaa ggcctgatta agccactggt tcatcctttg attgccctga tacgacacct
     1021 ttttccaatg acttcccgcg ccatttggct gtggacattg gtttggatga cgctcacaac
     1081 aggcttcttc atcagcaaac actcaggctt gcccgttttc acctcggctt ctgcactcat
     1141 gcaaatggcc gcgtcgcatc actccccatc agaccgctct acagtccaat ctgataccca
     1201 tgccgatgct gccaccaaca gccactcgtc cagtcatcag caaaactgcc aaactggcag
     1261 caatcagtcc tgccatgata ctgcccacca aagcaacgat tgcttatggc aatgcctgct
     1321 cgtatgcttg acgcagatgc tgttgtttgt aatgtgtctt gccatcggcc tgaaaattcg
     1381 tttgttggct tatcgcaaac tggctttgtc gttcaaatcc tcattgttaa aacgcttgtt
     1441 aaaacctccc aaaaacctga ctgtgtttgc ataaaaccct cagtcatatt tttaggagat
     1501 ttacatgatt aatcgacgcc gatttttatc atacggcttg ggcgtgggcg ccgcaggcct
     1561 tgctttcgcg tgcaacaaaa gcgcggccaa cagtgatgcg cacagcatgc acggcatgca
     1621 aaatgcctca gacaacacca catcccatca aaacatggac cacagcggcc atcaaatggg
     1681 tggcatgaat cacgccgcca atgagcaaga cttgctgccc gtgagcgcct tgagcacagg
     1741 ggcggctttg gctgctttgc cattgcttgc gaatgaatcc aagcaagcag gcaagtttac
     1801 aggccgctta atcgccaaaa aacacagcct caaattggct aaaggattgg aatctgaatt
     1861 ttggctgtat aacggcgtgt tgggtggccc acaaatcgtg gtgtatgaag gcgatgaagt
     1921 cagca
//
LOCUS       DAOMVK010000436         1924 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_17449_length_1924_cov_3.157838, whole genome shotgun sequence.
ACCESSION   DAOMVK010000436 DAOMVK010000000
VERSION     DAOMVK010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17449_length_1924_cov_3.157838"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PK856_11270"
     CDS             <1..322
                     /locus_tag="PK856_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HRL02989.1"
                     /translation="HYRTPKDVSVDGLDVLVTTNVLPWEAAIGGPITVKTPAGELQVN
                     VPAGSQTGKKLRLKGKGIPNKAEAGNLYLVLNVVVPPAVNEAQKQAYADLASAFPNFN
                     PRQA"
     gene            332..649
                     /locus_tag="PK856_11275"
     CDS             332..649
                     /locus_tag="PK856_11275"
                     /inference="COORDINATES: protein motif:HMM:NF024979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HRL02990.1"
                     /translation="MKHVTLHEITIAEVCYGEALDGEYTLNFEQFVHASGQEAAWVLQ
                     LFEHDILSSTAPPQRHQFVGDELTRARKAYRLQRDFDASLQAVAVMLDLIEEIQDLRK
                     RLP"
     gene            complement(731..1579)
                     /locus_tag="PK856_11280"
     CDS             complement(731..1579)
                     /locus_tag="PK856_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02991.1"
                     /translation="MKRSMVLVGMLLWQQMAMAEEFDGAWRHVAVPEGLAKTVQKMQT
                     LAKQPARAEFRARSKTWLRDLSWQQQTPVIQSEFAERKQRYSAPKYRMLSAKTVTQTR
                     LGMSDFVIMMSHRLHEDAPKEMTLWQMDMFADMGGKHFATQAEAVYHPVMGLMATWTD
                     NDEQSMQSHVLSQQQVGRLADGSLPRRYRETSHSEYRTLFELTPRERNGESECTASEP
                     YVGTRVHANIKGKVQTVQCITRENDHDANTIDMVFLLDYQVFVPTWQNYVGGQWQWRM
                     AEFEGT"
     gene            complement(1695..>1924)
                     /locus_tag="PK856_11285"
     CDS             complement(1695..>1924)
                     /locus_tag="PK856_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02992.1"
                     /translation="LQDYESDCIEADMLEAGESLPLAQMLEGASYGEQWLHRLPAEAK
                     ANVVICVFSPNRVSTPEACALPFIGQFSYIP"
BASE COUNT          454 a          544 c          464 g          462 t
ORIGIN      
        1 ccattaccgc accccaaaag acgtgagcgt ggatggcttg gatgtgttgg tcaccaccaa
       61 tgtgttgcca tgggaagccg ccatcggcgg ccctattacc gtcaaaaccc cagcgggtga
      121 gctgcaagtg aatgtgcctg caggcagcca aacgggtaaa aaactgcgcc tcaaaggcaa
      181 gggcattccc aataaagccg aagcaggcaa tttgtatttg gtgttgaatg tggtcgtgcc
      241 acctgccgtc aatgaagccc aaaaacaggc ttatgccgat ttggccagtg cctttcccaa
      301 tttcaacccg cgccaagcct aaggagacga catgaaacac gtgaccttac acgaaatcac
      361 catcgctgaa gtgtgttacg gggaagcttt ggatggcgaa tacacgctga attttgagca
      421 atttgtgcat gccagcggcc aagaagcggc gtgggtattg caattgtttg aacacgacat
      481 tctaagcagc actgccccac ctcagcgcca ccaatttgtc ggcgatgaat tgacacgcgc
      541 ccgcaaagcc taccgtttgc agcgcgattt cgatgccagt ttgcaagcgg tggcggtaat
      601 gctggatttg attgaagaaa tccaagactt gcgcaaacgc ttgccttaaa aggcttccct
      661 cacataaaca agcggcctgt aaacatttac aggccgcttt tatttgcttc actcacatca
      721 agtgcacggt ttaagtgccc tcaaattctg ccatgcgcca ctgccactgc cctccaacat
      781 agttttgcca agtgggcaca aaaacttggt aatccagcaa aaacaccatg tctatggtgt
      841 tggcatcgtg gtcattctca cgggtgatgc attgcacggt ctgcaccttg cctttgatgt
      901 tggcatgcac gcgcgtgccc acatacggct cactggcggt gcactcgctc tcgccattgc
      961 gctcgcgggg tgttaattca aacaaggtgc ggtattcgct gtgactggtt tcacggtagc
     1021 gccgcggcaa agagccatcg gccaaacgcc cgacttgttg ttgggacaaa acatggcttt
     1081 gcatcgactg ctcgtcattg tcagtccaag tcgccatcaa gcccatcacg gggtggtata
     1141 ccgcttcggc ttgggtggca aaatgcttgc cgcccatgtc tgcaaacatg tccatttgcc
     1201 acagcgtcat ttctttgggc gcgtcttcgt gcaagcggtg tgacatcatg atgacaaaat
     1261 cgctcatacc caagcgcgtt tgcgtcacgg tttttgccga caacatgcgg tatttgggcg
     1321 cagagtaacg ctgcttgcgc tcggcaaact cgctttgaat cacaggggtt tgctgctgcc
     1381 aagataaatc gcgcagccat gtttttgagc gcgcccgaaa ttccgctcgc gcaggttgct
     1441 tggccaatgt ttgcattttt tgcaccgtct ttgccaagcc ttcaggcaca gccacatgcc
     1501 gccaagcgcc atcaaactcc tctgccatcg ccatttgttg ccacaagagc attcccacca
     1561 acaccatact gcgcttcatc gcctcacctt tgcgtcttca agtgatgctt cttatatcac
     1621 agtttcatac cccgattaca agttaagcgg cctgtaaatg cttacaggcc gcttattctg
     1681 tcttttaaaa aaacttaagg tatgtaggaa aactgcccaa taaaaggcag cgcacacgct
     1741 tcaggagtgc tcacgcgatt ggggctgaac acgcaaatca ccacattggc cttggcctct
     1801 gctggcaaac ggtgtaacca ttgctcgcca tacgatgccc cttccagcat ttgcgccaaa
     1861 ggcagactct cgcccgcttc caacatgtcc gcctcgatgc aatctgactc ataatcttgc
     1921 aaac
//
LOCUS       DAOMVK010000437         1920 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_17507_length_1920_cov_2.914209, whole genome shotgun sequence.
ACCESSION   DAOMVK010000437 DAOMVK010000000
VERSION     DAOMVK010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17507_length_1920_cov_2.914209"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PK856_11290"
     CDS             complement(<1..274)
                     /locus_tag="PK856_11290"
                     /inference="COORDINATES: protein motif:HMM:NF017645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HRL02993.1"
                     /translation="MQNQLIAGFQNPVADAQAVFRTTLSAMSEPGLPQNLPMAAGLDS
                     LQAASWALLQALLDADVKVYLGDSVSTDKVRSNVLFHTGCELTNDKA"
     gene            complement(274..741)
                     /gene="phnG"
                     /locus_tag="PK856_11295"
     CDS             complement(274..741)
                     /gene="phnG"
                     /locus_tag="PK856_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HRL02994.1"
                     /translation="MYLSESIVWPSDATALRQMCLQVLAHQAPQLLAYQNELNQTEYE
                     WIRRPEVGMVMVHGRTEASGTPFAVGETTVSRCVLRLASGETGFGYVLGRDKLQAELI
                     ALADAHLQSEQQDYWMAHMMEPLAQQWKQAQAEHSANVADSRVDFFTMVRGED"
     gene            complement(752..1540)
                     /gene="phnE"
                     /locus_tag="PK856_11300"
     CDS             complement(752..1540)
                     /gene="phnE"
                     /locus_tag="PK856_11300"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HRL02995.1"
                     /translation="MNHVYVPPTSGGLKKLFNLLLWGLMLALLSLAWQGSEMAPIKLV
                     QDSGNMWTLITDFFPPDFKEWRSYLDEMLITIYIGIWGTLLAIVCAIPLGLMCAENIA
                     PKWMQIITKRCMDALRSINEMVFAMIFVAAVGLGPFPGVLALFLHSTGCLAKLFAEAV
                     EAIEPGPVEGVRATGADKIQEILYGVIPQVLPLWISYSLYRFESNVRSATVVGMVGAG
                     GIGVLLWESIRGFDFPATCAIMLIIIVTVTVIDALSQILRKRFI"
     gene            complement(1575..>1920)
                     /locus_tag="PK856_11305"
     CDS             complement(1575..>1920)
                     /locus_tag="PK856_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HRL02996.1"
                     /translation="SNAMAVANKQVDVGTFNTEGWAMMEKKNPDVLKKLKIVWKSPEI
                     PSDPLVWRKDMTDANKQKIKSFLMSYGSTPEEKKVLDALGWSKFRESNNDQLKPIREL
                     EEFKKQAAAKAA"
BASE COUNT          481 a          527 c          443 g          469 t
ORIGIN      
        1 gggctttgtc gttggtgagt tcacaaccag tgtgaaacaa cacattgctg cgcaccttgt
       61 ctgtgctcac gctgtcgccc aaatacactt tgacatcggc atcgagcaag gcttgtaaca
      121 aggcccaaga agcggcctgt aaactgtcta aacctgctgc catgggcaaa ttttgtggca
      181 agcctggttc actcatggcg ctcaaagtgg tgcggaacac ggcttgtgca tcggctacgg
      241 ggttttgaaa gcctgcaatc aattggtttt gcatcagtct tctcctctga ccatggtgaa
      301 gaaatcgaca cggctgtcgg cgacattggc gctgtgctcg gcctgtgctt gtttccattg
      361 ctgtgccaaa ggttccatca tgtgcgccat ccaatagtct tgttgctcag attgcaaatg
      421 cgcatctgcc aaggcgatca actcggcctg taacttgtcg cgacccagca cataaccaaa
      481 acctgtttca cccgaagcca agcgcaacac gcagcggctg acggtggttt cacccacggc
      541 aaacggtgta ccgctggctt cggtgcgacc gtgcaccatc accatgccca cctcggggcg
      601 gcgtatccat tcatactcgg tttggttcaa ttcgttttgg taagcaagca attgcggtgc
      661 ttggtgggcc aagacttgca aacacatttg gcgcaatgcc gtggcatcac tcggccatac
      721 gatgctttca gataaataca tgacacttct ttcaaataaa acgcttacgt aagatttgcg
      781 acaaggcatc aatcacggta acggtgacga tgatgatcaa catgatggcg caggtagcag
      841 ggaaatcgaa tccgcgtatg ctctcccaca acaacacccc aataccgccc gcacccacca
      901 tgcccaccac cgtggcagag cgcacattgg attcaaagcg gtataaagaa taggaaatcc
      961 acagcggcaa cacttgcgga atcacgccat acaaaatttc ttgaattttg tccgcacctg
     1021 tggcgcgcac accttcaaca ggaccaggct ctatcgcctc tacggcttcg gcaaacaatt
     1081 tcgccaagca acccgtgctg tgtaaaaaca atgccaacac cccagggaaa ggccccaagc
     1141 ccacggcggc cacaaaaatc atcgcaaaca ccatttcatt gatggagcgc agcgcatcca
     1201 tgcagcgctt ggtgatgatt tgcatccatt ttggcgcgat gttttccgca cacatcaaac
     1261 ccaaaggaat ggcgcagacg atggcgagta aagtgcccca aataccgatg taaatcgtga
     1321 tcagcatttc atccaaatag ctgcgccatt ctttgaaatc gggcgggaag aaatcggtaa
     1381 tcagcgtcca catattgccc gagtcttgca ccaacttgat gggtgccatc tcgctgcctt
     1441 gccatgccaa agacagcagc gccagcatca atccccacaa caacaggtta aacaattttt
     1501 tcaagccgcc cgaagtgggc gggacataca catgattcat ctatgaagcg tcctgtaaaa
     1561 aaacacgatg gtgtttatgc ggctttggca gcggcttgtt tcttgaattc ttccaattca
     1621 cggataggct tcaattgatc gttgttcgac tcacggaatt tgctccaacc caaggcatcc
     1681 aaaacctttt tctcttcagg cgtgctgccg taagacatca agaaagattt gattttttgc
     1741 ttgttggcat cggtcatgtc tttgcgccac accaatgggt ctgatgggat ttcaggtgat
     1801 ttccacacga ttttgagttt tttcaacaca tcaggatttt tcttttccat cattgcccaa
     1861 ccctcggtat tgaaggtgcc cacatctact tgcttgttgg ccactgccat cgcattgctt
//
LOCUS       DAOMVK010000438         1919 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_17518_length_1919_cov_2.311695, whole genome shotgun sequence.
ACCESSION   DAOMVK010000438 DAOMVK010000000
VERSION     DAOMVK010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17518_length_1919_cov_2.311695"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PK856_11310"
     CDS             complement(<1..193)
                     /locus_tag="PK856_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HRL02997.1"
                     /translation="MAMNWQQLLSAQRFKQIGDEVVPTKTPSTDEGVDALRTDFHIDY
                     DRVVFSGGFRRLGRKTQVHP"
     gene            607..960
                     /locus_tag="PK856_11315"
     CDS             607..960
                     /locus_tag="PK856_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL02998.1"
                     /translation="MNFDGIINELLKTDGAMAAAVVDYSSGMLLAGAGSSSMDLEIAA
                     AGNTEVVRAKMKTMKLLDLNETIDDILITLGEQYHLIRPIAKLDGLFLYFVLAKDKSN
                     LALARRALVDAEKHI"
     assembly_gap    1064..1121
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1146..1820)
                     /locus_tag="PK856_11320"
     CDS             complement(1146..1820)
                     /locus_tag="PK856_11320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HRL02999.1"
                     /translation="MPKAIFFDVDDTLLNFGLSSKGALADVFDRFGLNLDDAVQNRYD
                     DINNGLWAEQKRGLRSVQNVIDSRFRMLFDAMEWQVDVEAFRDAYQHNLAQSHVMEQG
                     ALETVRHLSQKYPLYVASNSILAMQTSRLRLAKLLPYFQDLFVSNDIGYEKPDLRFFE
                     TCLQRSQLQPEDIVFVGDSLEADMAGASGSGMTACWYNPQQKDAPQNIAIQHTITHLS
                     QLTELY"
BASE COUNT          529 a          458 c          375 g          499 t
ORIGIN      
        1 aaggatggac ttgggttttg cgccctaagc ggcgaaagcc acccgaaaac accaccctgt
       61 cgtaatcgat gtgaaaatca gtgcgcagtg catccacacc ctcatcggta gatggggtct
      121 tggtaggcac cacctcgtca ccaatttgtt tgaaacgctg cgcactcaat aattgctgcc
      181 aattcatcgc cataagtcac ctctgttgat attgggtatt ttacaggacg catgccatgt
      241 agacaaagcg gcctgtaaac cgatgtgact gtttttacgg catcgcggtg accaacaaat
      301 gacaatcctg ccccaatcca caagtctcgg ttcaaacacc cactgaacat tgaaagattg
      361 attacttgag tagccaacca ttttaacgaa atagacggct ggcatcgcac attactgttt
      421 ttaagcaacc catacttact gattgttcac atccacactt tgaaaaaata atgctgataa
      481 gagtttagaa cccattactc attcacattg acatattcct aaaatccatt catattagaa
      541 ccatctgcat cacacacgca ttgcgcgcag cagggtgctt ttacaaacac agaaatcgag
      601 agtcagatga attttgatgg cattattaat gagctgctaa aaaccgatgg ggcaatggcg
      661 gctgcagtgg tggattactc cagtggtatg ttattggcag gtgctggctc atccagtatg
      721 gatttggaaa ttgcggcagc aggcaatacc gaagttgtgc gtgccaaaat gaaaaccatg
      781 aagctattgg atttgaacga gaccattgac gatattttga ttaccttagg tgagcaatac
      841 cacttgattc gtcctatcgc caagttagac ggcttattct tgtattttgt tttggcgaaa
      901 gacaaatcca atttggcctt ggctcgtcgg gccttggtag acgctgaaaa acacatctaa
      961 ccgcccaatt aaaacacggt gttgcattca caacaccgtg ttttgctttg atatactctg
     1021 atttttcaat cacttgcact tgaaacacat cttatcatta cacnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntgattaaat acacatagct
     1141 gctgttcaat acaattctgt tagctgcgat aaatgcgtga tggtgtgttg aatggcgatg
     1201 ttttgcggcg catctttttg ctgcggatta taccaacaag cagtcatccc gcttccactt
     1261 gcgccagcca tgtctgcttc caagctgtcg cccacaaaca cgatgtcttc aggctgcaat
     1321 tgactgcgct gtaagcaagt ctcaaaaaat cgcaggtcgg gtttttcata gccgatgtca
     1381 ttggacacga acaaatcttg aaaatacggc aacaatttgg ccaagcgcaa acgcgaggtt
     1441 tgcatcgcca aaatgctgtt agaggccaca tacaatgggt atttctgact caaatgccgc
     1501 acggtttcca aggcaccttg ctccatcaca tgcgactggg ccaaattgtg ctgataggca
     1561 tcacgaaacg cctcaacatc cacttgccac tccatggcat caaacaacat ccgaaaacgg
     1621 ctgtctatga cgttttgcac gctgcgcaaa ccccgctttt gttcagccca caaaccgtta
     1681 ttaatatcat cataacgatt ttgcaccgca tcgtctaaat tcaaaccaaa gcgatcaaac
     1741 acatccgcta gagcgccttt gctgctcaat ccaaaattca acagcgtgtc gtctacatca
     1801 aaaaaaatcg ctttaggcat aaggtttact ttctccacaa ggtcaaacaa ggcttacaag
     1861 ctgtcattct agcccattaa atacccatgt gctgttttca aaatgtttac aggccgctt
//
LOCUS       DAOMVK010000439         1919 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_17519_length_1919_cov_2.220494, whole genome shotgun sequence.
ACCESSION   DAOMVK010000439 DAOMVK010000000
VERSION     DAOMVK010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17519_length_1919_cov_2.220494"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="PK856_11325"
     CDS             <1..669
                     /locus_tag="PK856_11325"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HRL03000.1"
                     /translation="VAYGHALTEGMLEAVYVEYARDLIVTISDTIEAVEIIHQALQLN
                     PQLRIIARAQNEEEQRMLLEAGASDVIYFHDVVAEKIIDLIQAEASVQEKAEDEIASK
                     MVAAAMRPDTQADSVLHPYTPASEMEGQECDAVEVEAVHGEEMPAALKQKSTMPNVTV
                     NTAHITQEADTPASEMEAQERETIIDESVAEVMSHDNQGLTDQEIERLALTEEQIKPQ
                     PQKD"
     gene            complement(743..>1919)
                     /locus_tag="PK856_11330"
     CDS             complement(743..>1919)
                     /locus_tag="PK856_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03001.1"
                     /translation="HIPHTFSTALVLKLGEQWVLPVGLLCDQFFEAEPQANLSHHIAN
                     IVQRELYFLSTDPTESMHLLLQLVQNHAQVPGGLAHAQAIHAHDWDYSLFSLEHLDQV
                     LQQVRSQHHFSNETWLKDSSEANFLMWASAYLASTIANMAQAALRWWNHADTSALMQQ
                     ELPESLASTRVAQIDQTLYFVMGYVSDYLLGNNEKSVSQYGQFVLSQLHNFPQTPIKP
                     LSQIKVEAPALWKNAMVNAGQTAAYMMHQLVNQPSQQAVLLPTLFDGSTFVQAMEKGE
                     TVEQHWSNNPQQRAYLSLAYEAHAYFATQRVDAIQIDTLVDDGSGSEFSVGITVPFFA
                     HTDYRGFQILRPYFTQLPTEQRQQWETLGEYFFNGIQQFELSQAPFWKSYYSGALIS"
BASE COUNT          516 a          413 c          512 g          478 t
ORIGIN      
        1 gtggcttatg gccatgcttt gaccgaaggt atgttggagg ccgtgtatgt ggaatatgcc
       61 cgcgatttga ttgtgaccat cagtgacacc attgaagcgg tagaaatcat ccaccaagca
      121 ctgcaattga atccgcaatt gcgtattatc gcccgcgcgc aaaacgaaga agagcagcgt
      181 atgctgttgg aggcaggtgc cagcgatgtg atttatttcc acgatgtggt ggctgaaaaa
      241 atcattgatt taattcaggc cgaagccagt gtgcaagaaa aggctgaaga cgagattgca
      301 tcgaaaatgg tggcggcagc aatgcgtcct gatacacaag ctgacagtgt tttgcatccg
      361 tatacgcctg cgagtgaaat ggaggggcaa gagtgtgatg ctgttgaagt agaggctgtg
      421 catggtgaag aaatgcctgc tgcgttgaaa caaaaaagca ccatgccgaa tgtgacggtg
      481 aataccgctc acatcaccca agaagcggat actccagcaa gtgagatgga agcacaagag
      541 cgagaaacca ttattgatga gtcggtggct gaggtgatgt cacatgacaa ccaaggtttg
      601 acggatcaag agatagagcg cttggccctg actgaagagc aaatcaaacc tcaaccgcaa
      661 aaagactgat tgcgggtgtc aaacaaacgg cctgtaaact tttacaggcc gcttgttttt
      721 gtggtgaaag gattgaaaga ccttaactga ttaatgcacc actgtaatag cttttccaaa
      781 atggcgcttg cgagagttca aactgttgga tgccattgaa gaaatactct cctaaggttt
      841 cccactgttg gcgctgttca gtgggcagtt gggtaaagta agggcgcaag atttgaaaac
      901 cgcggtaatc ggtgtgtgca aaaaatggca cggtaatgcc cactgaaaat tcactgcctg
      961 aaccatcgtc taccaaggtg tcaatttgga tggcatccac acgctgagtg gcaaaatagg
     1021 cgtgggcctc ataagccaag ctcaaatacg cacgttgttg cgggttgttt gaccaatgtt
     1081 gttcaacagt ttctcctttt tccatagcct gtacaaaggt cgagccgtca aataaggtgg
     1141 gcaataacac ggcttgttgg ctgggttggt tcaccaattg gtgcatcatg taagcggcgg
     1201 tttggcccgc attgaccatc gcatttttcc ataaggctgg ggcttctacc ttgatttggc
     1261 ttagtggctt gatgggcgtt tgcgggaaat tgtgcaattg tgacagcacg aattgaccat
     1321 attggctcac cgatttttca ttgttgccca gcaaataatc gctgacataa cccatcacaa
     1381 aatacaaggt ttggtcgatt tgagcgaccc gcgtagaagc caagctttca ggcaactctt
     1441 gctgcatcaa ggcactggtg tcagcatggt tccaccacct caaggctgcc tgtgccatat
     1501 tggcaatggt cgaggccaaa taggcactgg cccacatcaa aaagttggct tcactgctgt
     1561 ctttaagcca agtctcgttg ctgaaatggt gttgtgaacg cacctgctgt aagacttgat
     1621 ccaagtgttc aaggctgaac aatgagtaat cccaatcgtg agcatggatg gcttgggcat
     1681 gtgccaagcc gccaggtact tgcgcatgat tttggaccaa ttgcagcaat aagtgcatgg
     1741 attctgttgg gtctgtgctc agaaaataga gttcacgttg aacaatattg gcaatgtggt
     1801 gactgaggtt cgcctggggt tctgcttcaa aaaattgatc gcacaataaa cccactggca
     1861 aaacccattg ctcacccagt ttcaatacca aggccgtgct gaaggtgtgc gggatgtgt
//
LOCUS       DAOMVK010000440         1912 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_17603_length_1912_cov_3.298869, whole genome shotgun sequence.
ACCESSION   DAOMVK010000440 DAOMVK010000000
VERSION     DAOMVK010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17603_length_1912_cov_3.298869"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1912
                     /gene="glyS"
                     /locus_tag="PK856_11335"
                     /pseudo
     CDS             1..1912
                     /gene="glyS"
                     /locus_tag="PK856_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285867.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
BASE COUNT          466 a          517 c          525 g          404 t
ORIGIN      
        1 ggtgaaagct ttgccgccag catcatcgaa caattgcaaa aagaccactt catcgctgaa
       61 aacaccgaat acaaagtgtt tgcctcaccc cgccgcttgg gcgtgttggt gaaaaacgtg
      121 gcgtccatgc aagccgacca gcaagtggtg aaaaaaggcc cgtctgtggc caatggcatg
      181 aaagatggcg agccgaccaa agccttgttg ggttttgccc gcggtgcagg cgctgaagtg
      241 gcggatttga aagtcatcca cgatggcaaa caagacgtgt atgcctacga atacacccaa
      301 agcggccaaa gcttggaaag cgtgatggcc gatgtgttgg cgcaagcggt gaaaaaattg
      361 ccgattccca aagtaatgcg ttggggcagc tctacccaca ccttcgtgcg ccctgtgcat
      421 ggcttggtgg tgatgttggg caaaaccgtg ttgaacaccg aagtgttggg ccttaccagc
      481 caaaactgga ctttgggtca ccgcttcttg gccactgagc cagtggtgtt taccgatgcc
      541 aacgaatacg aaagcaaaat ggctgaaaaa ggcaaagtaa tcgctaattt caccgctcgc
      601 cgcgccttga ttgacgagca tttggctcaa gcagctggcc gacgaggctt tgcgcgacga
      661 agtaacggcg ttggtagaat tgcctgtggt gctagaagca ggttttgaag cacatttctt
      721 gaatgtgcca caagaatgct tgattttgac catgcaacaa aaccaaaaat acttcccctt
      781 gctcgacgcc aatggcaagt tgatgaaccg ctttttgctc gtgtccaaca tcaaagccca
      841 agaccctgtg catgtgatta cgggcaatga gcgcgtgttg cgcgcgcgtt tgtctgatgc
      901 cgaattcttc taccaaaccg acttgaaaaa gccgttggaa agccgtttgc cgcgtttgca
      961 aaacgtggtt taccacaaca aattgggcag ccaaagcgaa cgcattgcgc gcttggtcgc
     1021 gattgcaggc aaggtcgcca gcaaaatcgg tgcagacttg gcccaaaccg aacgcgcaac
     1081 cttgttggcc aaagccgact tggtgaccga aatggtcggt gaattcccag aattacaagg
     1141 cacgatgggc aagtattacg cccaacacga tggcgaaacc gccgtggtgg cacaagcttt
     1201 ggagcaacac taccaaccgc gttttgctgg cgatgccttg cctgaaacca cagtggccac
     1261 cagcgtggcc ttggccgaca agctcgaagc catcgtcggc atttggggca taggcttgat
     1321 tccaacaggc gacaaagacc cttacgcctt gcgtcgcgcc gctttgggcg tggtgcgcat
     1381 gttgatgcaa taccctgtga gcttgccagc cttggtgcaa gacgcgtttg acagcttccc
     1441 tgtgggcaaa ctggccgcca acaccgtcgc cgaagtcacc gactttatgt acgcgcgttt
     1501 ggccgtattg ttgcaaggcg attacgccca agacgtggtg gccgccgtat tggcccaccg
     1561 cccagacagc ttgcacgacc tcaccgcgcg tttagaagcc gtgagcgaat tcaaagcctt
     1621 gcctgaagcc gccgctttgg ccgccgccaa caaacgcgtg cacaatttgc tcaagaaaac
     1681 cgacggcgaa ttgggcgcgg tcaatcctgc cttgttgcaa gaagacgctg aaaaagcctt
     1741 gtttgccgcc actgaaggct tacaggccgc catagccagc gccatgaacg agcaaaattt
     1801 caaagccgct ttgagccaat tggcgacttt gcgcgcccct gtcgatgcct tttttgacgg
     1861 cgtgatggtg atggccgacg atgcggcctt gaaacaaaac cgcttgaact tg
//
LOCUS       DAOMVK010000441         1905 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_17695_length_1905_cov_2.396757, whole genome shotgun sequence.
ACCESSION   DAOMVK010000441 DAOMVK010000000
VERSION     DAOMVK010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17695_length_1905_cov_2.396757"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1234
                     /locus_tag="PK856_11340"
     CDS             <1..1234
                     /locus_tag="PK856_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008028 - monocarboxylic acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HRL03002.1"
                     /translation="PVAITAALLVGLGFNPLYAAGLCLIANTAPVAFGAVGVPIIVAG
                     QVSGLDPYDVGQLAGRQLPILSIIVPFWLIAMMDGVRGIKQTWPAVLVCGVTFAITQF
                     LTANFIGPELPDITSALVSLICLALFLKKWQPKEIFTFAGMKPLNREANKSEYTGGQI
                     FKAWSPFIILTAMIALWSIKDVQGLLKFATIEFEFPGLHNLVMKTAPIVAENTPYAAM
                     FKWNILGASGTSILITAIITSVLLKFSASNFIKTFFETLYELRLSVFSIGLVLGFAQV
                     ANYSGLSSTLALVLAGTGALFPFFSPFLGWLGVFLTGSDTSSNALFGSLQANTANHLG
                     LDPTLAVAANTTGGVTGKMISPQSIAVACAAVGLQGQESKLFRFTVKHSLVFCTFVGI
                     LCVIQEYVMPWTLHLFTK"
     gene            1522..>1905
                     /locus_tag="PK856_11345"
     CDS             1522..>1905
                     /locus_tag="PK856_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03003.1"
                     /translation="MHTTLKLLSLSSLLALSHYAQADWVDDAVAANNAHVIQLREHIH
                     QHPELGNIEFETSKLVQKELKSYGIEVKTGYAKTGVIGILKGAKPGPVMAMRADMDAL
                     PMPEKTGLAYASKAKAMYQGKESPVM"
BASE COUNT          462 a          469 c          423 g          551 t
ORIGIN      
        1 ccctgtggcg attaccgcgg ctttattggt aggtttgggc tttaacccac tctacgcagc
       61 gggtctgtgc ttgattgcca acaccgctcc tgtggccttt ggtgccgtgg gtgtacccat
      121 tattgtggct ggccaagttt caggcttaga cccttacgat gtgggccaat tggcaggtcg
      181 tcaattgcct atcttgtcta tcatagtgcc attctggtta atcgccatga tggacggcgt
      241 gcgcggcatc aaacaaactt ggcctgcggt attggtgtgt ggcgtgactt tcgccatcac
      301 ccaattctta acggccaact tcataggccc agaattgcca gacatcactt ctgctttggt
      361 gagcttgatt tgcttggcct tgttcttgaa aaaatggcag ccaaaagaaa ttttcacttt
      421 tgcaggcatg aaaccattga atcgtgaagc caacaaatct gaatacacgg gcggacaaat
      481 tttcaaagct tggtctccat tcatcatctt gaccgccatg attgccttgt ggtctatcaa
      541 agatgtacaa ggcttgttga aatttgccac cattgaattt gaattcccag gcttgcataa
      601 cttggtgatg aaaaccgctc cgattgtggc agaaaacacc ccttacgctg ccatgttcaa
      661 atggaacatc ttgggtgcca gcggtacatc tattttgatt actgccatca tcaccagcgt
      721 gttgttaaaa ttcagcgcct ctaacttcat caaaaccttc tttgaaacct tgtatgaatt
      781 gcgcttatcg gtattctcca tcggtttggt attgggtttt gcccaagtag ccaactattc
      841 tggcttgtct tctaccttgg ctttggtttt ggctggcaca ggcgctttgt tcccattctt
      901 ctcaccattc ttgggttggt tgggcgtgtt cttgactggt tcagacactt cttcaaatgc
      961 cttgtttggc tctttgcaag ccaatactgc caaccacttg ggcctagacc cgactttggc
     1021 cgtggctgcc aacaccactg gtggcgtgac aggtaagatg atttcgccac aatccatcgc
     1081 agtggcttgt gcggcggtgg gtttgcaagg tcaagaatcc aaattgttcc gcttcaccgt
     1141 gaagcacagc ttggtattct gtacttttgt cggcatcttg tgtgtgatac aagaatatgt
     1201 gatgccttgg acattgcact tgttcaccaa gtaagtttta aaccttaaag aaacaccctg
     1261 cttgtacatc aagcagggtg tttcgcttac aggccgcatc ttttcaaagc gaccatccaa
     1321 atgttttttt aaaatctcaa tatctttgtc gctttaaaag cctaatccgg cgcattctta
     1381 cccatttcat ttttgcacac cacagtatgc tttttggcta tacaatcaga tgtcaaaagt
     1441 atgcaagcct gattgcctct gtgttttaat ctcaacagct gaatcaaaat cagcactttt
     1501 caatacacaa agaagaacac catgcacacc actttgaaac tgttaagcct gagcagcttg
     1561 ctcgctcttt ctcattacgc ccaagccgat tgggtagacg atgccgtcgc agccaacaat
     1621 gctcatgtga ttcaattgcg cgaacacatc caccaacacc ctgagctggg caacatcgaa
     1681 tttgaaacct ccaaactggt gcaaaaagag ctcaagtctt acggcataga agtcaaaaca
     1741 ggttatgcca aaacaggtgt gattggcatt ttaaaaggtg cgaaacctgg cccagtgatg
     1801 gcgatgcgcg ccgacatgga tgccttgccc atgcctgaaa aaacaggctt ggcttatgcc
     1861 agtaaagcca aggccatgta ccaaggcaag gaatcgcctg tgatg
//
LOCUS       DAOMVK010000442         1900 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_17759_length_1900_cov_2.924661, whole genome shotgun sequence.
ACCESSION   DAOMVK010000442 DAOMVK010000000
VERSION     DAOMVK010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17759_length_1900_cov_2.924661"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="PK856_11350"
     CDS             <1..731
                     /locus_tag="PK856_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL03004.1"
                     /translation="KIEPSTQATHQTEFVLNEKTEGDEAPLKALFQHRRTFSTLMFWV
                     AFFMCLLMVYALGSWLPKLMLQAGYSLGASMLFLFALNIGGMVGAIGGGVLADRFHLK
                     PVLTIMFTIGAVALILLGFKSQQAVLYTLIAIAGAATIGSQILLYTFVAQFYPAAVRS
                     TGMGWASGIGRIGAIVGPVLTGALLTLELPHQMNFLAIAIPGFIAALAIFLVNLKASV
                     DGQHTPSIAGTDAVASSSATAYNK"
     gene            complement(812..1666)
                     /locus_tag="PK856_11355"
     CDS             complement(812..1666)
                     /locus_tag="PK856_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRL03005.1"
                     /translation="MAYQTRQQLEQTHAVLHNSITISSGMQLAQWSNQRDHVAVLSDH
                     HTLSMYVREGYESYQKTAHGWKNGGGPGRFCLMPQAQHSEWDIRGELGFVHLYFTDAH
                     LHDIATQTWDQDPQNIVLHEHSFGQDAHIAQLYQLFLLQADWQNPADHLQLSSASTLL
                     LHHLIKHYGNVNWRSPHSKGGLSPHSLKRVAAYVDAHLQDALTLQDLANIACLSPYHF
                     AHMFKQSTDLSPHQYVLKRRLEQAHTLILHSPHSLLQIAQQCGFNNASHFSRRFKQHF
                     GVLPSQLR"
BASE COUNT          422 a          453 c          536 g          489 t
ORIGIN      
        1 acaaaatcga gccgagcaca caggccaccc accaaactga atttgtattg aatgaaaaaa
       61 ccgagggtga cgaagcgcca ttgaaagcct tgttccaaca caggcgcacc ttcagcacct
      121 tgatgttttg ggtagccttc tttatgtgct tattgatggt gtacgctttg ggcagctggt
      181 tgccaaaatt gatgttacag gccggttatt cattgggcgc gagcatgctg ttcttgtttg
      241 ccttgaacat aggcggcatg gtgggtgcaa tcggtggcgg cgtgttggca gaccgttttc
      301 acttgaaacc cgtgctcacc atcatgttta ccataggcgc ggtggctttg attttgttgg
      361 gctttaaaag ccaacaagcg gtgttgtaca ccttaatcgc cattgcaggc gcagccacca
      421 tcggttcgca aattctgctc tatacctttg tggcgcagtt ttacccagcg gccgtgcgct
      481 cgacagggat gggttgggct tcgggcatag gccgcatcgg cgcgattgtc ggccctgtgt
      541 tgacaggcgc attgttgact ttggaattgc cgcaccaaat gaatttcttg gccatcgcca
      601 tcccaggctt catcgcggct ttggcgatat ttctggtgaa tttgaaagcc tctgtggatg
      661 ggcaacacac gcccagcatc gcaggcacag atgcggttgc cagtagcagt gcgactgctt
      721 ataacaagta atggttcaaa accacagctg aaacacattt acaggccgct tgatgcggcc
      781 tgtaagcctt taaaaacctt gattgaatgg gctaacgcaa ttgcgatggc aacacgccaa
      841 agtgttgctt aaagcggcgg ctgaaatggc tggcattgtt aaagccgcat tgctgcgcaa
      901 tttgtaacaa ggaatggggg ctgtgcaaaa tcagcgtgtg cgcctgctcc aagcgccgtt
      961 ttaacacgta ttggtgcggc gacaaatcgg tagactgttt gaacatgtgg gcaaaatggt
     1021 aggggcttag gcaagcaatg tttgccaagt cttgcaaggt gagcgcgtct tgcaggtgtg
     1081 cgtccacata agccgccacc cgtttcaagc tgtgcggcga caagccgcct ttgctgtgtg
     1141 ggctgcgcca attcacattg ccatagtgtt tgatgaggtg gtggagcagc aaggtgctgg
     1201 cgctgctcaa ttgcaaatgg tcggcaggat tttgccaatc ggcttgcagc aaaaacaact
     1261 gataaagctg ggcaatgtgg gcatcttggc caaacgagtg ctcgtgcaac acaatgtttt
     1321 gtgggtcttg gtcccacgtt tgcgtggcga tgtcgtgcaa atgcgcatcg gtaaaataca
     1381 ggtgcacaaa acccaattct ccgcgtatgt cccattcgct gtgctgcgct tgcggcatca
     1441 aacaaaacct gcctggcccg ccgccgtttt tccaaccgtg ggccgttttt tggtagcttt
     1501 catagccttc gcgcacatac atgctcaagg tgtggtggtc gctcaatacc gctacatggt
     1561 cgcgctggtt gctccactga gccaattgca tgcccgagct tatggtgatg ctgttgtgca
     1621 gcaccgcatg ggtttgctcc aattgttgtc gggtttggta agccatggtg ttgagtactg
     1681 agccaaaaac aaggcttatt gtaggcgagt ttgcgggttt acaggccgca tttcctgatc
     1741 cctccccaaa aatacacaag aacagaacag ggatacgcaa gtttggcgca gccgtgagcc
     1801 aattgtgtgt gcatactgtg gccacgatgg aatttgagac cagacatgaa tgcattgttg
     1861 tatgtggcgg tggtgttgat ttggggcacc acttggatag
//
LOCUS       DAOMVK010000443         1892 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_17872_length_1892_cov_1.949374, whole genome shotgun sequence.
ACCESSION   DAOMVK010000443 DAOMVK010000000
VERSION     DAOMVK010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17872_length_1892_cov_1.949374"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="PK856_11360"
     CDS             complement(<1..970)
                     /locus_tag="PK856_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HRL03006.1"
                     /translation="MHKQTVKGWIIIAIAAALSLGLYQILPYDVAANKGLALLTFIAI
                     LWLTEAVHITITALLVPVLAVIIGMPMENARKELVPLTTTKALATFADPTIFLFFGGF
                     ALATALHVQKLDRKIAMWIISLSRGHLGVAVMAIFAVTAALSMWISNTATAAMMLPLA
                     LGILSHLDASKERKTFVFILLGIAYSASIGGLGTIVGSPPNAIAAKALDYDFADWMKV
                     GLPMMFIILPAMLLSLFLILRPKLNQKITFHTEDIPWTGSRIAAMVLFVCTAVAWIFS
                     GQLTEALGLSQTDTWIALLASTLVVVLGTATWKQISDNTDWGVLMLF"
     gene            complement(1485..>1892)
                     /locus_tag="PK856_11365"
     CDS             complement(1485..>1892)
                     /locus_tag="PK856_11365"
                     /inference="COORDINATES: protein motif:HMM:NF012273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HRL03007.1"
                     /translation="MAFINSSYTTSFIPLWLSMEVDSLSETDTSVLHKIGTMLTNKSQ
                     KQLEHLVPASPFFYGNTPTLTDFLFAGVGRWADFHQVFDLQAYPKTAQWKEAIFDLPQ
                     IQLAEQIEQGSFSQTDFGQEIALDVALSLRQQS"
BASE COUNT          558 a          475 c          426 g          433 t
ORIGIN      
        1 caaacagcat caacacgccc cagtcggtat tgtcggaaat ttgcttccaa gtggccgtac
       61 ccaacactac cactaaagtc gaggccaaca aggcaatcca agtgtcggtt tggctcaagc
      121 ccaaagcctc ggtcaattgg ccgctgaata tccatgccac agcagtgcag acaaacaaca
      181 ccatcgcggc gatgcgtgag cctgtccacg gtatgtcttc cgtgtgaaac gtgatttttt
      241 ggttcaattt cggacgcaaa atcaaaaaca agctgagcaa cattgcgggc aagatgatga
      301 acatcatcgg taagcccact ttcatccaat cggcaaaatc ataatccaaa gccttggccg
      361 caatcgcatt cggtggcgaa cccacgatgg tgcccaagcc acctatgctg gccgaatagg
      421 cgatacctaa caagatgaac acaaaggttt tgcgctcttt gctcgcatcc aaatgcgaca
      481 aaatgcccaa ggccaaaggc aacatcatcg ctgcggtggc ggtgttggaa atccacatcg
      541 acaaggccgc ggtcacggca aaaatggcca tcaccgccac gcccaaatgc ccgcgtgaca
      601 aagagataat ccacatcgca attttgcgat caagcttttg cacatgcaaa gccgtggcca
      661 aggcaaagcc gccgaaaaac aaaaagatgg tggggtcggc aaaagttgcc aaagccttgg
      721 tcgtggttaa aggcaccaac tctttcctgg cattctccat aggcatacct atgatgacgg
      781 ccaacactgg caccaataag gcggtgatgg tgatgtgcac cgcctcagtc agccacaaaa
      841 tggcgatgaa tgtgagtaag gccaaacctt tgttggcggc cacgtcataa ggcaaaatct
      901 gatacaagcc cagtgatagt gcggcggcga tggcaatgat gatccagccc ttcaccgttt
      961 gtttgtgcat gacattggtt ggcgtggcca aattcttgtc atccatgcgc gtctccttta
     1021 gtacaatccg attccaatcg attgccaaat agttatggca atcgacaaag cttcgcattt
     1081 tccgtctaaa aatcacaaaa gccaagcatc aattgctaaa caattgacag gatatgcaat
     1141 tttaaaatca aaatcagcct aaaaagcgca tgcatgctat ttaatggcag tcaacctacc
     1201 atagaaaatg attgataaca caaacgcttt actgacaaag caatacgaac atggcaaaca
     1261 agcgcccgtt ttgcctaacc tttctaataa accatccgag aaaagccctc attacagcca
     1321 tgggcatgat tcaagcaatg cttgcacttg aggtgcccat tatcagtgca aaacgctcaa
     1381 acagtgggca cctgtgtaca cttcagcctg ctgcttcaga acttcaggat aaatttggct
     1441 gatgactgcc tatcttgaat aacaaactta ttgatctagt caactcaaga ctgttgacgc
     1501 agacttaatg ccacatctaa agcgatttct tgcccaaaat cggtttggct gaagctgcct
     1561 tgctcgattt gttcagccaa ttggatttgt ggtaaatcaa agatggcctc tttccactgg
     1621 gcggttttgg ggtaggcctg caagtcaaaa acctgatgaa aatctgccca cctgccgaca
     1681 cctgcgaaca gaaaatccgt taaagtcggg gtattgccat agaaaaatgg gcttgcggga
     1741 acaaggtgtt ccaattgctt ttggctttta ttggtgagca tggtgccgat tttgtgcaga
     1801 accgaagtgt cggtctcgct caaactgtca acctccatgc tcaaccacaa aggaatgaaa
     1861 gaagtggtgt aagaggaatt gataaacgcc at
//
LOCUS       DAOMVK010000444         1888 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_17924_length_1888_cov_2.491544, whole genome shotgun sequence.
ACCESSION   DAOMVK010000444 DAOMVK010000000
VERSION     DAOMVK010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17924_length_1888_cov_2.491544"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="PK856_11370"
     CDS             complement(<1..721)
                     /locus_tag="PK856_11370"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HRL03008.1"
                     /translation="MGYILALDQGTTSSRSMVFDAQLNVVASAQRPLPIHTPQTGWVE
                     QDAEEMWAGQMATIQEALSRAGLLASDIAAIGIANQRETTVVWHKKTSKPIAPALVWQ
                     DRRTQTWCEAQAHMAARVQERTGLRLDPYFSASKLVWLLNHVPAARAMAEAGELLFGT
                     VDTWLVWQLTRGKRHIIDASNASRTLLMNLHTGTWDEELCAAWDIPIAMLPEIVPSSG
                     DLARTASGLLAAEIPITAILGD"
     gene            831..>1888
                     /locus_tag="PK856_11375"
     CDS             831..>1888
                     /locus_tag="PK856_11375"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HRL03009.1"
                     /translation="MKSIRLSACAGALMMLISAESFAKDWPTLNGEPPIVVAHRGASA
                     YLPDHTLEAYRRAVELGADFIEPDVVSTKDGVLIVRHEPNLKDTTDIAEQPEFASYKR
                     KQKVDGKEEEGWFANDLTYAQIQTLRAKQPMAQRSQQYNGQFKIASLEEVLQLREQLS
                     RETGRTIGVYPETKHPAWHAEHGLGFENKLVALLNQYQLNQKSSPVFIQSFELGNLKA
                     LKRLTPVRLVYLLDGDDVAANGEVKTMVPYDFQLSGDKRTYADMLSNANLREIKQYAD
                     AIGPWKVYIMSYKTDKNGVKTQLTPTDVVKRAHQQGLQVVPFTFRSDAKYLTATENGN
                     PSIEYQQYFKLGVDGVFS"
BASE COUNT          437 a          533 c          487 g          431 t
ORIGIN      
        1 ggtcgcccaa aatcgccgtg atggggattt cggctgccaa caagcctgat gctgtcctag
       61 ccaaatcgcc actggagggc acaatttcgg gcaacatggc gatggggatg tcccacgcgg
      121 cgcacaattc ttcgtcccac gtgcctgtgt gtaggttcat cagcaaggtg cgcgaagcat
      181 tggaggcatc gatgatgtgg cgtttgcccc gtgtcaattg ccacaccagc catgtgtcta
      241 ccgtgccaaa cagcaattcg cccgcctctg ccatggcgcg cgctgcgggc acatggttca
      301 gcagccacac caatttgctg gcgctgaaat acgggtctag gcgcaggcct gtgcgctctt
      361 gcacccgcgc cgccatgtgc gcttgcgctt cgcaccatgt ttgggtgcgc ctgtcttgcc
      421 acaccaaagc aggcgcgatg ggcttgcttg tttttttgtg ccacaccacc gtggtttcgc
      481 gctgattggc aatgcctatg gcggcaatgt cgctggccaa caagcctgcg cggctgagcg
      541 cttcttgtat ggtcgccatt tgccctgccc acatttcttc agcgtcttgc tccacccagc
      601 ctgtttgcgg cgtgtgtatt ggcaagggcc gctgcgcact ggccaccaca ttcaactgtg
      661 catcaaacac catgctgcgg ctggaagtgg tgccttggtc gagcgcgagt atgtatccca
      721 tgttgtgtcc ttgtttacag gccgcttgtt tgcgcctgta ttcatcgtat tgtcatgtct
      781 gggttttagc atggcacttg catctttatc aaccacactt taaggtaagc atgaaatcca
      841 tccgtttaag cgcctgcgca ggagccttga tgatgctcat cagtgccgaa tcatttgcca
      901 aagattggcc cactttaaat ggcgagccgc ccatcgtggt ggcccaccgc ggtgcgtcgg
      961 catatttgcc cgaccacacc ttagaagcct accgccgcgc ggtagaattg ggtgccgatt
     1021 tcattgagcc cgatgtggtt tctaccaaag acggtgtgtt gattgtgcgc catgagccca
     1081 atctgaaaga caccaccgac attgccgagc agcctgaatt tgcaagctac aaacgcaagc
     1141 aaaaagtgga tggcaaagaa gaagaaggct ggtttgccaa cgatttgact tacgcccaaa
     1201 tccaaacctt gcgcgccaaa caaccgatgg cgcagcgcag ccagcaatac aatggccaat
     1261 tcaaaatcgc gagcttggaa gaagtgttgc aattgcgcga acaattgtcg cgcgaaacag
     1321 gccgcaccat aggcgtgtat cccgaaacca aacaccctgc atggcatgcc gagcatggct
     1381 tgggctttga gaacaaactg gtggccttgt taaaccagta tcagctcaat caaaaatcct
     1441 cacccgtgtt cattcaatcg tttgaattgg gcaatttaaa agccttgaaa cgcctcacgc
     1501 ctgtgcgctt ggtgtattta ttagatggcg atgatgtcgc cgccaatggc gaagtgaaaa
     1561 ccatggtgcc gtatgatttt cagctcagtg gcgacaagcg cacctatgcc gacatgctca
     1621 gcaatgccaa tctgcgcgaa atcaagcaat acgccgatgc cataggcccg tggaaagtct
     1681 acatcatgtc ttacaaaacc gataaaaacg gcgtgaaaac ccaattaaca cccaccgatg
     1741 tggtcaaacg cgcccaccag caaggcttgc aagtggtgcc gtttaccttc cgcagcgatg
     1801 ccaaatacct caccgccacc gaaaatggca atccatctat tgaataccaa caatacttca
     1861 aactgggtgt ggacggcgtg ttcagcga
//
LOCUS       DAOMVK010000445         1879 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_18045_length_1879_cov_2.728618, whole genome shotgun sequence.
ACCESSION   DAOMVK010000445 DAOMVK010000000
VERSION     DAOMVK010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18045_length_1879_cov_2.728618"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PK856_11380"
     CDS             complement(<1..469)
                     /locus_tag="PK856_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03010.1"
                     /translation="MSDTDKTLVLHTSLWAVVRFVLPNLCWIVAAVVSAGLIYSEFSR
                     HPFVEETRKYWLGLFVFVWSVTFGCAALLKTRVLLYAWPRFYTQIQLSPQGLRLQNWP
                     HNQHFIAWTDIQAIQDIYPHKKMPARYAQLTVAPEHYHRLQRESHIDKPYFTQE"
     assembly_gap    538..547
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            858..1283
                     /locus_tag="PK856_11385"
     CDS             858..1283
                     /locus_tag="PK856_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03011.1"
                     /translation="MLYAWSYWLWSLFTEGSGNITSLMFIAVFILLPVTAGVIQSAIE
                     ILSLAGNNKPLLTLNEQGVVYKNQVVHWKDIASIQIGRQYRHEHRIPDIALYLHPRAM
                     ESQHNAIMWIALRHVKVEEYLLMRQLKHYWHLYREVAAA"
     gene            complement(1372..1743)
                     /gene="rbfA"
                     /locus_tag="PK856_11390"
     CDS             complement(1372..1743)
                     /gene="rbfA"
                     /locus_tag="PK856_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRL03012.1"
                     /translation="MKKQQRGYARQDRVQEQIMRDLAELTRTGLKDPRAGFITITEVE
                     LTRDYSHATVWYTVMDEKTREVTAEALDNAKGHLRSELAKRIKVFKTPELHFEYDESI
                     ERGMNISSLIDQANSEKPVQD"
BASE COUNT          482 a          372 c          476 g          539 t
ORIGIN      
        1 cttcttgggt gaaatacggc ttatcaatat gggattcgcg ctgtaagcgg tgataatgtt
       61 cgggtgcaac agttaactgt gcgtatcttg caggcatttt tttatgtgga tagatgtctt
      121 ggatggcctg tatgtctgtc cacgcgatga aatgctgatt gtgtggccag ttttgcaaac
      181 gtaaaccttg aggtgacagc tgtatttggg tgtaaaagcg cggccatgcg taaagcaaca
      241 cacgggtctt aagcaatgct gcacaaccaa atgtcaccga ccacacaaag acaaacaatc
      301 ccaaccaata ttttcgcgtt tcttccacaa acggatggcg cgaaaattca ctgtaaatca
      361 aaccagcact cacaacagca gcaacaatcc agcacaggtt tggcaagacg aaacgcacaa
      421 cagcccagag actggtatgc agcaccaagg ttttgtccgt gtctgacatg tagactgtgt
      481 gagtcggctt gggcttgaag aacatgtgca atcaaagtgg tggcttgaga tgccttannn
      541 nnnnnnntga caagtttgga taaggttagg ctcatttcag ggcaaaaaag tttggacgtt
      601 tatctggatg atgtggctac tacagtcaat tatctgggga taaaggtagt ttatattatg
      661 gcatggtagg attgattgcc ctgatttgtt acgcatgttg attaacattg tatgctgcct
      721 gtgcagaata ttttcaggat ggaaaaggaa gggcgtatga gccacgaaca gcaaacaacc
      781 ttgccaatac actattttgg caaaagtggt ttggcgctgt tgaagctgcc atttgtaatg
      841 gtgtttgtgc tgtgtgtgtg ttgtatgcat ggagctattg gttgtggtct ttgtttactg
      901 aaggcagcgg taacattacc tcactgatgt ttattgctgt gtttattttg ttacctgtga
      961 ctgcgggggt gattcaaagt gccatagaaa ttttgagctt ggcaggcaat aacaagccct
     1021 tattgacatt gaatgagcaa ggtgtggttt acaaaaatca agtcgtgcat tggaaggaca
     1081 ttgccagcat acaaatagga cgacagtatc gacatgagca ccgtattcct gatatcgctc
     1141 tttatttaca tccaagagcc atggagtcgc aacacaatgc catcatgtgg attgcactgc
     1201 gccatgtgaa ggtggaggag tatttactga tgcgacagct gaaacactat tggcatttgt
     1261 accgagaagt cgcagcagct taatcagact aattatggtt tacaggccgc ttatggaata
     1321 taagcggcct gtaaacattt aaaacctatt caatacatga tgactaccac atcagtcttg
     1381 cacaggtttt tcgctgttgg cttggtcaat taagctggag atgttcatgc cgcgttcgat
     1441 ggattcgtcg tattcaaaat gcaattctgg ggttttgaac actttgatgc gtttggccaa
     1501 ttctgagcgc aaatggcctt tggcattgtc caaggcttcg gcggtgactt cgcgggtttt
     1561 ttcgtccatc acggtatacc acacggtggc gtggctgtag tcgcgcgtga gctcaacttc
     1621 ggtgatggtg atgaaaccag cacgtgggtc tttcaaaccc gtacgggtta attctgccaa
     1681 gtcgcgcatg atttgctctt gtacgcggtc ttggcgggca tagcctcgtt gttgtttttt
     1741 catgttgctt tcctaatata aaagctgtcc agaggcttaa gcatccagac agctgatgtt
     1801 tacaggccgc ttaagtgatt acaagctgcg agccacttca acgatttcga agaattccaa
     1861 ggtatcgccg atgatgatg
//
LOCUS       DAOMVK010000446         1878 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_18055_length_1878_cov_3.145365, whole genome shotgun sequence.
ACCESSION   DAOMVK010000446 DAOMVK010000000
VERSION     DAOMVK010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18055_length_1878_cov_3.145365"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(233..>1878)
                     /locus_tag="PK856_11395"
     CDS             complement(233..>1878)
                     /locus_tag="PK856_11395"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HRL03013.1"
                     /translation="KIVKNGETIANFELKSQVLFDEVQAGGRINLIIGRGLTGKAREA
                     LGLPASEAFRLPQAPAATNAGFTLAQKMVGRACGLPEGEGVRPGTYCEPKMTTVGSQD
                     TTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVKTHKELPEFISTRGGVALR
                     PGDGVIHSWLNRLLMPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPES
                     VLVRFSGNLQPGVTLRDLVNAIPLYAIKAGLLTVAKAGKINIFSGRILEIEGLPDLKV
                     EQAFELTDASAERSAAGCTVKLNKEPIIEYMQSNVVLMKNMIADGYADARTLARRIES
                     MEKWLANPNLLEADANAEYAAVIEINMDDVKEPILACPNDPDDVKILSDVAGTKIDEV
                     FIGSCMTNIGHFRAAGKLLEGKADIPVRLWVAPPTKMDAEQLSDEGYYGILGRAGARM
                     EMPGCSLCMGNQAQVREGATVVSTSTRNFPNRLGKNTYVYLSSAELAAIASKLGHIPT
                     VEEYFQNVAIINEKGAEVYRYMNFNQIEKYETVAEQVNI"
BASE COUNT          514 a          470 c          430 g          464 t
ORIGIN      
        1 gcctcatggc gagacaaata acggtattcc aattgccatt cagcacggcg tttgctctcc
       61 cttaccagca aaaacgcaat gaaaacaagc aatattaata aggcgatcca aaacatatgc
      121 aatataggtg ttatttttgt agacaggtga aagcattgta cctgttttca tggcctgtgc
      181 atcaaaaaag ccccatcgtt tgatggggct aagtcacaat caactcagtt gattaaatgt
      241 tcacttgctc agcaacggtt tcatattttt caatttgatt gaaattcatg taacggtaca
      301 cttctgcacc tttttcgttg atgatggcga cgttttggaa atactcttcc acggttggga
      361 tgtgacccaa tttggacgca atcgccgcca attcagcaga gctcaaatac acataggtat
      421 ttttgcccaa acggttaggg aagttacggg ttgaggtaga caccacagtc gcaccttcgc
      481 gcacttgtgc ttggttaccc atgcacaatg agcaacccgg catttccatg cgcgcgcctg
      541 cacgacccaa gataccgtaa tagccttcgt cagacaattg ttcagcatcc attttggttg
      601 gaggagctac ccacaagcgt acaggaatgt cggctttgcc ttctaacaat ttaccggccg
      661 cacggaagtg accgatgttg gtcatgcaag aaccgatgaa cacttcatca attttggtac
      721 ccgcaacatc tgacaagatt ttcacgtcat cagggtcgtt agggcaagcc aagataggtt
      781 ctttcacatc gtccatgttg atttcgatga cagcagcgta ttcagcattg gcatcggctt
      841 ccaacaagtt aggattggcc aaccattttt ccatgctttc gatgcggcga gccaaagtgc
      901 gggcatcggc gtaaccatcg gcaatcatgt ttttcatcaa taccacgttg gactgcatgt
      961 attcgatgat aggctctttg ttgagtttca ccgtacaacc cgcagcagaa cgctcagcag
     1021 aagcatcggt caattcaaat gcttgctcaa ctttcaaatc aggcaagcct tcgatttcca
     1081 aaatgcggcc tgagaagatg ttgattttgc cggctttggc aaccgtcaat aaacctgctt
     1141 tgatggcata caaaggaatg gcattcacca agtcacgcaa agtcacgcct ggttgcaaat
     1201 taccagagaa gcgcaccaat acagattcag gcatgtccaa aggcatcaca ccagtggccg
     1261 cagcaaacgc caccaagcca gaaccagctg ggaatgaaat accaatcggg aaacgggtgt
     1321 gagagtcacc accagtcccc acagtatcag gcatcaacaa acggtttaac catgagtgaa
     1381 tcacgccatc acctggacgc aatgccacac cgccacgggt tgagatgaac tcaggcaatt
     1441 ctttgtgggt tttcacgtca acgggtttag ggtaagcagc tgtgtggcag aatgattgca
     1501 tcaccaagtc agcagagaag cccaagcaag ccaagtcttt caattcgtca cgggtcatag
     1561 gacctgtggt gtcttgagag ccgactgtgg tcattttagg ttcacagtaa gtacctggac
     1621 gcacgccttc gccttcgggc aaaccacaag cgcggccaac cattttttgc gccaaagtaa
     1681 agccagcatt ggttgcagca ggggcttgtg gcaagcggaa tgcttcagaa gcaggcaagc
     1741 ccaaggcttc acgtgccttg ccagtcaaac cacggccaat gatcaagtta atacggccac
     1801 cggcttgaac ttcgtcaaac aatacttgcg atttcaattc gaaattggca atggtttcac
     1861 catttttaac gattttgc
//
LOCUS       DAOMVK010000447         1878 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_18056_length_1878_cov_3.057049, whole genome shotgun sequence.
ACCESSION   DAOMVK010000447 DAOMVK010000000
VERSION     DAOMVK010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18056_length_1878_cov_3.057049"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="PK856_11400"
     CDS             <1..700
                     /locus_tag="PK856_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRL03014.1"
                     /translation="VIVAIATPSAQSVVAATREVPVVFAAVTDPVAAKLVTSWEPSGT
                     NVTGVSDELPLEPQIDLMKKLVPNVKTVGYVYSPGEVNSTVILEKLKIQLKTQGIEVI
                     GAPAQRTTDISTAANSLAGKVQMIYTTTDNNVVSGYESLYKVAVANKIPLLASDTDTV
                     KRGASAALGVNYHDMGMQTGKIVTRILKGEKPGAIATQKMEKLDLFVNPKAAQSQGFT
                     IPEELVKSAKEVIQ"
     gene            825..1730
                     /locus_tag="PK856_11405"
     CDS             825..1730
                     /locus_tag="PK856_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRL03015.1"
                     /translation="MSMIALFGALETGLIYALVALGVYISFRILDFPDLTADGSFPLG
                     GAVCAVCIVNGVNPWLACGLAIVAGGLAGMLTAWMHVSLKILQLLAGILVMVALYSVN
                     LRIMGSPNVPLIGEPSVYSGFIGDKFDNQYWIQPLVILGFVIAAKFLMDWFFSTQTGL
                     AMRATGANARMARAQGVATNKMVILGMALSNGLIALGGALFVQTQGGADISIGIGTIV
                     IGLAAVIIGETLIPSKKFLIITLSVVIGAVLYRLFIALALGSETLRNIGFGPQDLNLV
                     TALLVVLALRFPQIKSFFKKMGKAS"
     gene            1727..>1878
                     /locus_tag="PK856_11410"
     CDS             1727..>1878
                     /locus_tag="PK856_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019388922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRL03016.1"
                     /translation="MMQAQDLRLTFNPGTPIENPALRGMSLNINEGEFVTVIGSNGAG
                     KSTFLNS"
BASE COUNT          445 a          431 c          487 g          515 t
ORIGIN      
        1 tgtgattgtg gccattgcca cgccgagcgc gcagtctgtg gtagcggcta cacgcgaagt
       61 gccagtggtg tttgctgcgg taaccgaccc tgtggccgcc aaattggtaa cgtcttggga
      121 gccttctggc accaacgtga ccggtgtatc tgacgaattg ccattggaac cacaaatcga
      181 tttgatgaaa aaattggtgc ctaatgtgaa aaccgtgggc tatgtttaca gcccaggcga
      241 agtgaattca accgtgattt tggaaaaatt gaaaatccag ttgaaaaccc aaggcattga
      301 agtgatcggc gcacctgcac aacgcaccac cgatatttct actgcagcca acagcttggc
      361 tggcaaagta caaatgatct acaccaccac cgacaacaat gtcgtgtctg gttatgagtc
      421 tttgtacaaa gtggccgtgg cgaacaagat tcctttgttg gcatcagata ccgataccgt
      481 taaacgtggc gcttctgcgg cattgggcgt gaattaccac gacatgggca tgcaaacagg
      541 taagattgtg acccgtattt tgaaaggtga aaaacctggc gcgattgcca ctcaaaaaat
      601 ggaaaaattg gatttgtttg tgaatccaaa agccgcacag tctcaaggct tcaccattcc
      661 agaagaattg gtgaaatctg ccaaagaagt gattcaataa gtcatcacag catttaaaag
      721 aactgccaac tgaagacagc agcacatcct ttggttggca tttttgattc aggccgctta
      781 cccagcgact tgcatcggta tttaaccgac aaaagaagat ccttatgtcc atgatagctt
      841 tgtttggggc tttagaaact ggcctcattt acgccttggt ggcgttgggg gtgtacatct
      901 ccttccgcat ccttgatttc cctgatttga ctgccgatgg ctccttccct ttggggggcg
      961 ctgtgtgcgc agtgtgcatc gtgaacggtg tgaacccttg gttggcgtgt ggcttggcca
     1021 tcgtcgcggg cggtttggct ggcatgctca cggcttggat gcacgtatca ctgaaaatct
     1081 tgcaattgct ggcaggtatt ttggtgatgg tggccttgta ttcggtcaat ttgcgcatca
     1141 tgggttcgcc caatgtgcct ttaatcggtg aacccagcgt ttacagcggc tttattggtg
     1201 acaagtttga caaccaatac tggatacagc ctttggtgat tttgggtttt gtgattgcag
     1261 cgaaattttt aatggactgg ttcttctcta cccaaacagg tttggcgatg cgtgccacag
     1321 gtgccaatgc acgcatggcg cgcgcgcaag gcgtggccac caataaaatg gtgatcttgg
     1381 gcatggcttt gtccaatggc ttgattgcat tgggcggcgc tttgtttgtg caaacccaag
     1441 gcggtgccga catttccatt ggtattggca caatcgtgat tggtttggcg gcggtgatta
     1501 ttggtgaaac cttgatccca tcgaaaaaat tcctcatcat caccttgtct gtggtgatag
     1561 gtgccgtgct ctaccgcttg ttcattgcct tggctttggg cagtgaaacc ttacgcaata
     1621 ttggatttgg tccacaagat ttgaacttgg taacggcatt gctggtggtg ttggccttgc
     1681 gtttcccaca aatcaaatcc ttctttaaga aaatggggaa agcatcatga tgcaagccca
     1741 agacttacgt ctgactttta accctggcac ccccatcgaa aacccagcct tgcgtggcat
     1801 gagcctgaac atcaatgaag gcgaatttgt caccgtgatt ggttctaacg gtgcgggcaa
     1861 atccaccttt ttgaattc
//
LOCUS       DAOMVK010000448         1876 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_18083_length_1876_cov_2.874794, whole genome shotgun sequence.
ACCESSION   DAOMVK010000448 DAOMVK010000000
VERSION     DAOMVK010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18083_length_1876_cov_2.874794"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="PK856_11415"
     CDS             <1..621
                     /locus_tag="PK856_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002381057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRL03017.1"
                     /translation="IAASGKQGQTLLNEAISRGLDVTAIVRDASKISQNVPVLEKDVL
                     ALSRGDLAAFDVVINAFGAPSELSHLHVEVGQHLIALLQGTTTRLLVVGGAGSLFVNA
                     EHSLRLIDTPEFPDVYKPIASNQGQNLQDLQAASDLNWVFLSPAAFFDPAGKRTGRYT
                     LGGDELLLNSEGNSYVSYADYATAMIDEATTPKHLNQRFSIVGEAT"
     gene            694..1191
                     /locus_tag="PK856_11420"
     CDS             694..1191
                     /locus_tag="PK856_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009426775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HRL03018.1"
                     /translation="MQKIISSIVVTSLALGISSAAWAKRVPVQNTQQHNTGLSATERV
                     NRQVVVDFYQQVFQKHQVEDAVKQHIGKEYIQHNPHVGDGVEPFVAYFVPYFQQNPQA
                     RSDIKRVIVQGDLVVLHVFSREHPQDRGTAVVDIFRVKDGKIVEHWDVQQAVPEDAAN
                     SNTMF"
     gene            1324..1812
                     /locus_tag="PK856_11425"
     CDS             1324..1812
                     /locus_tag="PK856_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03019.1"
                     /translation="MSQTHTQTTLNVDEVIIEHEPLNHLPVRTESVSEQAAATEPNKR
                     EQARQKRAHLLAEQAKLQQMLDDIEDDGAAEIADLQQQVTALKAEVAAWQAPENPHSV
                     LAQIGLFALTASALCWLLAPLLAGQGEYLHGSMSIAATLLAYLAGALLLFVVASGNGK
                     KH"
BASE COUNT          485 a          471 c          504 g          416 t
ORIGIN      
        1 attgccgcca gcggcaaaca aggacaaaca ttattgaatg aggccatcag ccgtggcttg
       61 gatgtgactg ccatcgtgcg cgatgccagc aaaatcagcc aaaacgtgcc cgtgctggaa
      121 aaagacgtgt tggccttaag ccgcggcgat ttggcagcgt ttgatgtggt gattaacgcg
      181 tttggcgcgc caagcgagtt gtcacacctg catgtggaag tgggccagca tttgatagcc
      241 ttattgcaag gcacaactac gcgtttgttg gtggtgggcg gcgcgggcag tttgtttgtc
      301 aatgcggaac acagcttgcg cctgatagac acgcctgagt ttcccgatgt ttacaaaccg
      361 atagccagca atcaaggcca aaacctgcaa gatttacagg ccgcatctga tttgaattgg
      421 gtatttttaa gccctgcggc tttttttgac cctgcaggta aacgtacagg ccgatacacg
      481 cttggcggcg atgagttgct gctcaacagc gagggcaaca gctatgtgtc gtatgccgat
      541 tacgccaccg ccatgattga tgaagccacc acgcccaaac acctcaatca gcgcttcagc
      601 attgtgggtg aagccacata acatattcca ttgggaatct atgcgcaaac ccccacactc
      661 gtaaaccagt tgaaaacggt aaaaggaaaa aacatgcaga aaatcatcag cagcatcgtg
      721 gtgaccagtt tggccttggg catcagcagc gcggcttggg ccaagcgcgt gcctgtgcaa
      781 aacacacagc agcacaacac aggcttgagc gccaccgaac gcgtgaatcg ccaagtggtg
      841 gtggattttt atcagcaagt gtttcaaaaa caccaagtgg aagacgcggt gaagcaacac
      901 ataggcaaag aatacatcca acacaatccg catgtaggcg atggcgtaga gccatttgtg
      961 gcgtattttg tgccgtattt tcagcaaaac ccacaagcgc gtagtgacat caagcgcgtg
     1021 attgtgcaag gtgatttggt ggtgttgcat gtgttttcac gcgagcaccc acaagaccgc
     1081 ggcacggcgg tggtggacat cttccgtgtt aaagacggca aaattgtcga gcattgggat
     1141 gtgcaacaag ctgtgcctga agacgctgcc aacagcaata ccatgtttta aacccatgtg
     1201 atgcgtcaca agcaagcggc ctgtcaacat ttacaggccg cttattgcgt tcactgggct
     1261 tgctcaaata aggtttgtgg ttataatcaa ggccaacatc cccgtccacc aagaggtcgc
     1321 tgtatgtcac aaacccacac ccaaaccaca ctgaatgttg atgaagtcat catcgaacac
     1381 gagccgctca accatttgcc tgttcgcact gagtctgtca gcgagcaagc ggccgctaca
     1441 gagccgaata agcgtgaaca agcgcggcaa aagcgcgcgc atttgctggc tgagcaagcc
     1501 aagctgcaac aaatgttgga cgacatcgaa gacgatggcg cggccgaaat cgccgatttg
     1561 caacaacaag taaccgcttt aaaagccgaa gtcgcggcat ggcaagcgcc tgaaaacccg
     1621 cattcggtgt tggcacaaat tggcttgttt gcgttgacgg cgtcggcatt gtgctggctg
     1681 ctcgcgcctt tgttggcagg acagggcgag tatttacatg gctctatgtc catcgcggcc
     1741 acattgctgg cctatttggc cggtgcgttg ctgctgtttg tggtggccag tggcaatggc
     1801 aaaaagcatt aaacgcgtta tttgagaatc ctgcattcct ttgagagtgc ctataagcgg
     1861 cctgtaaaca tttaca
//
LOCUS       DAOMVK010000449         1874 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_18106_length_1874_cov_3.752062, whole genome shotgun sequence.
ACCESSION   DAOMVK010000449 DAOMVK010000000
VERSION     DAOMVK010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18106_length_1874_cov_3.752062"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="PK856_11430"
     CDS             complement(<1..652)
                     /locus_tag="PK856_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HRL03020.1"
                     /translation="MVNLYQLLGIEMNASNEDIQAAIEQHQALGQINPEVLEKASAWL
                     LDGATRARYDAQLRGSDASLGNTPIPDLDISPRLTTPNLMTSKPASIQQAAPTSAPIA
                     QPRSDGSQDGPPLPDTQELFTALVGESKADYYLAEFDAMSAGKKSRFNWATFFGGSYW
                     FAARGMWGMAILILCVASAALILTAILASTLGNAGWVLLVLWLLWRLVWLPLKANQT"
     gene            864..1319
                     /gene="dut"
                     /locus_tag="PK856_11435"
     CDS             864..1319
                     /gene="dut"
                     /locus_tag="PK856_11435"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRL03021.1"
                     /translation="MSDAQVQLKIINPQMAEQLPEYATAGSAGLDLRAAVDAPLTVAA
                     GQTVLIPTGLAMYLQNPNLAAMILPRSGLGHKNGIVLGNLVGLIDSDYQGELKVSLWN
                     RSQEDFVVEPLARIAQLIIVPVVQAQFEIVDEFVASDRGEGGFGSTGHH"
     gene            1391..>1874
                     /gene="mnmA"
                     /locus_tag="PK856_11440"
     CDS             1391..>1874
                     /gene="mnmA"
                     /locus_tag="PK856_11440"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004286062.1"
                     /note="catalyzes a sulfuration reaction to synthesize
                     2-thiouridine at the U34 position of tRNAs; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRL03022.1"
                     /translation="MIWQTQQLPPHTKVIVGLSGGVDSSVTAALLKQQGHQVKGVFMQ
                     NWEDDNNDEYCSIKQDSLDAMAVADILDVDIDIVNFAGEYKDRVFAYFLQEYQAGRTP
                     NPDVLCNAEIKFKSFLDYALEQGAEVMATGHYARKAVVDDVHYLLKGLDGNKDQSYFL
                     Y"
BASE COUNT          475 a          488 c          443 g          468 t
ORIGIN      
        1 aggtttgatt ggctttgagt ggcaaccaaa ccaaacgcca taacaaccac aacaccagca
       61 aaacccaacc agcattgccc aaggtgctgg ccaaaatggc tgtcagtatc aaggccgcag
      121 acgccacaca caaaatcaaa atggccatgc cccacatgcc gcgggctgca aaccaataac
      181 tgccaccaaa gaaagtcgcc cagttaaaac ggcttttctt gcctgcagac atggcatcaa
      241 attcggccaa ataataatca gccttgcttt cacccaccaa ggcggtgaac aattcttggg
      301 tatcaggcaa tggcggccca tcctgactgc catctgagcg tggctgcgca atgggggcac
      361 ttgttggtgc ggcttgttga atactggcgg gttttgaggt catcagattc ggcgtggtaa
      421 ggcgcgggct gatgtccaaa tcaggaatgg gcgtgttgcc caagcttgca tcactgccac
      481 gcaattgcgc gtcatagcgc gctcttgtgg cgccatccaa aagccatgca ctggcttttt
      541 ctaacacctc aggattgatt tgccccaaag cctgatgctg ctcaatagcg gcctgtatgt
      601 cttcattgct ggcattcatc tctatgccca agagttggta caaattgacc atgttttccc
      661 cttagttttt ggccattgct ttgaagcttt cgctcaagac gccattattg gtgaaattct
      721 tgattttgtg aagttgctat gccttagccc ctccgccgtt gtgtgacagc ctctttctca
      781 ctttaaccgc atcgcccatg aagtttgtgg tggtttagcg taaaatggtg tctttaaatt
      841 tttccttgaa aacgaatccc tccatgtctg acgcgcaagt acaactcaaa atcatcaatc
      901 cccaaatggc cgaacaattg cctgaatacg caaccgcagg ctctgctggc ttggatttgc
      961 gcgcggcagt ggatgcgcct ttaaccgtgg cagcaggcca aaccgtgtta attcccacag
     1021 gtttggccat gtatttgcaa aaccctaact tggccgccat gattttgcca cgctcaggct
     1081 tgggccacaa aaatggcatc gtgctcggca atttggtggg cttgattgat tcagactacc
     1141 aaggcgaatt gaaagtatcg ctgtggaacc gctcacaaga agattttgtg gtggaaccac
     1201 tcgcccgcat cgcccaactc atcatcgtac ctgtggtaca agcacagttt gaaatcgttg
     1261 acgaatttgt cgccagcgac cgcggcgaag gcggctttgg ctctacaggc caccactaaa
     1321 ctcttgtaac tagattactt agtttaattt tctacagtaa aaccctaata cttatggcat
     1381 agtgcatcac atgatttggc aaacacaaca actcccccct cataccaaag tcatcgtcgg
     1441 tttgtccggc ggtgtggact cctctgttac cgcggcttta ttgaagcagc aaggccatca
     1501 ggtcaagggt gtgttcatgc aaaattggga agacgacaac aacgatgagt attgctccat
     1561 caagcaagac tcactcgatg cgatggcggt ggccgacatc ttagatgtgg acatcgacat
     1621 cgtgaatttt gcgggcgagt acaaagaccg tgtgtttgcc tattttttgc aagagtatca
     1681 agcaggccgc acccccaatc ccgatgtgct gtgcaatgcc gaaatcaaat tcaaaagctt
     1741 cttggactat gccttagaac aaggcgcaga agtgatggcc acagggcatt acgcccgcaa
     1801 agcagtggtg gatgacgtgc attacctgct caaaggcttg gatggcaaca aagaccaaag
     1861 ctatttttta tacc
//
LOCUS       DAOMVK010000450         1865 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_18235_length_1865_cov_2.369061, whole genome shotgun sequence.
ACCESSION   DAOMVK010000450 DAOMVK010000000
VERSION     DAOMVK010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18235_length_1865_cov_2.369061"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PK856_11445"
     CDS             <1..195
                     /locus_tag="PK856_11445"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRL03023.1"
                     /translation="GKALLLHGLQALQAEGRQTVDIEATLTGVAFYQKHGFRILEQTQ
                     SKHGGADAPLAVVKMQWQAA"
     gene            376..873
                     /locus_tag="PK856_11450"
     CDS             376..873
                     /locus_tag="PK856_11450"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRL03024.1"
                     /translation="MSKWLVWGVMLYSFAASAQEGVADVASEDIDASYSLHADDAPVG
                     VITTYISKNRTEEAQPAMVNPSSASQYTSFGIGPSVKLGEHVTAYGVIGAAADNRSNF
                     DTAMPVQALTLDNDARMYGVGMQIRPNQDWQIDMKYQNANLNSGADATRQVNYFNVGI
                     GYKFW"
     gene            complement(961..1218)
                     /locus_tag="PK856_11455"
     CDS             complement(961..1218)
                     /locus_tag="PK856_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03025.1"
                     /translation="MNKFIATLVFTLAASVATVSMAAPAAPAHPAQHLSKPVTHVQKK
                     TVKKPTAKKPVAKKHAVKNHTSSKPVNPKHVVKKPAPHQAH"
     gene            complement(1678..>1865)
                     /locus_tag="PK856_11460"
     CDS             complement(1678..>1865)
                     /locus_tag="PK856_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRL03026.1"
                     /translation="RHLHALQAAAQELAFAADCGEMIDLLAEHLRLAQVCLNEITGEF
                     TADDLLGVIFSRFCIGK"
BASE COUNT          461 a          424 c          518 g          462 t
ORIGIN      
        1 ggcaaggctt tgctcttgca tggtttgcag gccttacagg ccgagggcag acagacggta
       61 gacattgaag cgactttgac cggtgtggcg ttttaccaaa aacatggctt tcgcattctt
      121 gagcaaaccc agtcgaaaca cggcggtgcg gatgcgccgt tggcagtggt gaagatgcaa
      181 tggcaagcgg cttaaacgga taccgtgaat tggggtaatg acacggtcaa ccagccgatg
      241 ccagcgcaaa aaaaacactc agacaagcgg cctgtaaact tcttaatcgg tgattttcat
      301 tgaccgatta gagcaaatac cgttatggta agcgctcgtg gcttttgtca gcaatgtcaa
      361 tcatttggag tgggcatgag caaatggctg gtgtggggtg tgatgctgta ctcgtttgca
      421 gcaagcgcgc aagaaggtgt ggccgatgtc gccagtgaag acatcgatgc cagctatagc
      481 ctgcacgccg acgatgcgcc tgtcggcgtc atcaccactt acatcagtaa aaaccgcacc
      541 gaagaagcgc aaccagccat ggtcaaccca agctctgcca gccaatacac ctcgtttggc
      601 atcggcccca gcgtaaagct gggcgagcat gtcaccgcct atggtgtgat aggtgcggcc
      661 gccgacaacc gcagcaattt cgatacggcc atgcctgtgc aagcgctgac tttggacaac
      721 gatgcgcgca tgtatggcgt gggcatgcaa attcgcccca atcaagattg gcaaatcgac
      781 atgaaatacc aaaatgccaa cctgaacagc ggcgcggatg ccacgcgcca agtcaattat
      841 ttcaatgtcg ggataggcta taaattttgg tgaggctttt gtgcctgagc caagagcctt
      901 acttttaaat caacaagcgg cctgtaaaca gtttacaggc cgcttgtttt gtctgccgca
      961 tcaatgggct tggtgtggcg cgggtttttt aaccacgtgt tttgggttca cgggttttga
     1021 agaagtgtga tttttgactg cgtgtttttt cgccactggt tttttggcag taggcttttt
     1081 cacggttttt ttctgaacgt gtgtgacagg tttgctcagg tgttgggcag ggtgggctgg
     1141 cgcagcagga gcggccatgc tgacggtggc cacactggcg gcaagagtga atacaagggt
     1201 ggcaatgaat ttgttcatgt tttttcctaa gccaagcaag ggctggtttg ttgtgactgg
     1261 gtttttggtg ttgtagccat cttataaaac gaaacttagc ttggactgag gaaaacgtca
     1321 gattgggtta atttttgcac cagaggaaaa cggtggcgca gccatgtaca ttctgtgaac
     1381 aagaaggttt aaccgcagaa ccatgttggt atgaatgaga ttgatgtgtc atgaaaggat
     1441 gggtggcttg gcgtggcgtc aggctatatc ggtgtagaaa aggcggctac caaacaggct
     1501 taataatttt tggtgctaag cattgaagca tcgattcttt ttcaaacggg gctcaaggcg
     1561 ctaggatgac aaacattgac tcacgcaatg cgcaagttaa tgttctcctt ctcattcaat
     1621 ggatgttttg cggcgttctg caaggtgcag aacgccactt tttttgcagc ccaagcctta
     1681 tttgccgatg caaaaacggc tgaaaatcac accgagcaaa tcatcggcgg taaattcacc
     1741 cgtgatttca ttcaaacaca cttgcgccaa gcgcaaatgc tcggccaaca aatcaatcat
     1801 ctcaccgcaa tcggcggcaa acgccagctc ttgcgcggcg gcctgtaagg catgcaaatg
     1861 gcggg
//
LOCUS       DAOMVK010000451         1863 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_18259_length_1863_cov_2.345133, whole genome shotgun sequence.
ACCESSION   DAOMVK010000451 DAOMVK010000000
VERSION     DAOMVK010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18259_length_1863_cov_2.345133"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="PK856_11465"
     CDS             <1..588
                     /locus_tag="PK856_11465"
                     /inference="COORDINATES: protein motif:HMM:NF013788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C13 family peptidase"
                     /protein_id="HRL03027.1"
                     /translation="MFARKGLDPANQHHLNSQFPDVLMPKEDQASFWGLSRSLSQLNV
                     QPKQDACLFYVSSHGNKDRGVYLKRAMGRELTPNQLNTALNWTCGSAPTIVMVSACYS
                     GQFVAPLRGDNRIIMTAAAAHRPSFGCSEDNEYTFWDGCIISNFEQSSTWENLYHNVQ
                     QCITRKEQQLGYPASQPQAYFGKNMRGLKLDFHQN"
     gene            complement(674..1780)
                     /gene="hppD"
                     /locus_tag="PK856_11470"
     CDS             complement(674..1780)
                     /gene="hppD"
                     /locus_tag="PK856_11470"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HRL03028.1"
                     /translation="MGFEPWDNPMATNGFEFIEYAAPDPVVMGKIFEQMGFKAIARHR
                     HKNITLYRQGEINFIVNAEPNSFAQRFARLHGPSVCAIAFRVDNAQHAYERAIELGAW
                     GVPSGSGPMELNIPAIKGIGDSLIYLVDRWRGKQVGGIGDIDIYDVDFVSINPETAAE
                     DIHHQGAGLSYIDHLTHNVHKGRMVEWAEFYERLFNFREVRYFDIEGKVTGVKSKAMT
                     SPCGKIRIPINEEGTAEKGQIQEYLDMYHGEGIQHIALGTAVIYDAVETLRDNGVKFL
                     NTPDTYYELLDKRLPDHGEDVPRLHHNKILLDGGPDNDLLLQIFTVNQLGPIFYEIIQ
                     RKGNEGFGEGNFKALFESIELDQMRRGVVKQVPE"
BASE COUNT          476 a          476 c          411 g          500 t
ORIGIN      
        1 atgttcgcgc gcaaaggttt ggaccccgcc aatcaacacc atttgaattc acaatttccc
       61 gatgtcttaa tgccaaaaga agaccaagcg tctttttggg gtttaagccg cagcttaagc
      121 caattgaatg ttcagcccaa gcaagatgct tgcttgtttt atgtgtcgtc acatggtaac
      181 aaagaccgtg gtgtgtattt aaaacgtgcc atgggcagag aactcacccc caatcaactg
      241 aacacagcat tgaattggac ttgtggctct gcccccacca tcgtcatggt ctcagcttgt
      301 tattcaggcc agtttgttgc cccattgcgc ggcgacaacc gtatcatcat gacggctgct
      361 gccgctcata gaccctcatt tggttgctcg gaagacaatg aatacacatt ttgggatggc
      421 tgtatcatca gcaattttga acaaagcagc acttgggaaa acctttatca caacgtacag
      481 caatgcatta cgcgcaaaga gcagcaactg ggctaccctg cttcacagcc gcaagcctat
      541 tttggtaaaa acatgcgtgg cttaaaactg gattttcacc aaaattaagt gctgaaccac
      601 acaccttcac agacaaataa gcggactgta aactttacag gccgcttatg acgggcttat
      661 gttgatgaag ccatcattct ggcacctgtt tcaccacccc acggcgcatt tggtccaatt
      721 ctatcgattc aaacaaggct ttaaaattgc cctcgccaaa gccttcattg cctttgcgtt
      781 ggataatctc gtagaaaatc ggccccaatt ggttgactgt gaatatttgt aacagcaaat
      841 cgttgtctgg cccgccatca agcaaaatct tattgtggtg caaacgcggc acatcctcgc
      901 cgtggtctgg caagcgtttg tcgagcaatt cataataagt gtcgggcgta tttaaaaact
      961 tcacgccatt gtcacgcaag gtttccactg catcgtaaat caccgcagtg cccaaggcga
     1021 tgtgttgtat gccttcgccg tggtacatgt cgagatactc ttgaatttgc cctttttccg
     1081 ccgtgccttc ttcattgatg gggatgcgaa ttttgccaca aggtgaggtc atcgccttgg
     1141 atttcacccc tgtgaccttg ccctcaatgt caaaataacg cacttcacgg aaattgaaca
     1201 agcgctcata aaactcagcc cactccacca tacggccttt gtgcacattg tgggtcaaat
     1261 ggtcgatgta gcttaagcca gctccttgat ggtgtatgtc ttcagccgcg gtttcgggat
     1321 tgatggagac aaaatccaca tcataaatgt cgatgtcacc gatgccaccc acttgcttac
     1381 cgcgccaacg gtctaccaaa taaatcaacg aatcgcctat gcctttaatg gcaggaatgt
     1441 tcaattccat agggccactg cccgacggca cgccccaagc ccctaactct atggcacgct
     1501 catacgcatg ttgggcattg tccacccgaa acgcaatcgc acacactgaa ggcccatgca
     1561 aacgagcaaa acgctgggca aacgaattgg gctcggcatt gacgatgaaa ttgatttcac
     1621 cttggcggta taaagtgatg tttttgtgac ggtggcgggc gatggctttg aagcccattt
     1681 gctcgaaaat tttgcccatg accactgggt ctggcgcggc atattcgatg aattcaaagc
     1741 cattcgtcgc catagggttg tcccaaggtt caaatcccat ggtgtctcct ttgttttttg
     1801 tagagggttg gtttcttgtt tttctgtgtt tcgctattaa aaatcaattt acctgataaa
     1861 aat
//
LOCUS       DAOMVK010000452         1862 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_18269_length_1862_cov_2.972883, whole genome shotgun sequence.
ACCESSION   DAOMVK010000452 DAOMVK010000000
VERSION     DAOMVK010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18269_length_1862_cov_2.972883"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="PK856_11475"
     CDS             complement(<1..618)
                     /locus_tag="PK856_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRL03029.1"
                     /translation="MSNTMTFALDNQDQLTLQRLSGPLGANFDALSKSLNVEIRRAAD
                     ALLIKGEDAAVAMACLQQLLGLAQQRELTDTDIQLSVVAAKAQQVSEQVQHEFRERER
                     EQPYNEKQQYSFRTRRGNLTGRTPRQNGYIKALHNHDVVFGLGPAGTGKTYLAVAAAV
                     DAMERHLVDRIVLVRPAVEAGEKLGFLPGDLAQKVDPYLRPLYDAL"
     gene            852..>1862
                     /locus_tag="PK856_11480"
     CDS             852..>1862
                     /locus_tag="PK856_11480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRL03030.1"
                     /translation="MQSKILILMCAVLLGLAACGKGDNAAKSPQTPAAKQLSAADVLT
                     MQAQDFAQVVAFTGSLKPIKEATVSAESGGTVAKLLVDEGNAVQAGQVIAVMDNQAVR
                     ESLQGQQAQVQNSQANLDLAATKLKQQQVLFGKGFVSKLALEQAQNEYKVALGSHKAQ
                     QAELSKLNKNQADANVRAPISGGVYDKLVNAGELVNAGGQILKIAQTNVLEIAATVSS
                     EQVTFVKEGQEVKFTVATGAPEHAGRIVRINPVADANTRQFTVFIQVQNADGAVKAGQ
                     FAQGNIVLNQVAQAMVVPETAIKHAEGQAFVYVVSQGKLAKRPVKIVLQDPSTARAAV
                     EGL"
BASE COUNT          468 a          459 c          536 g          399 t
ORIGIN      
        1 cagagcatcg taaagcgggc gcagatacgg gtcgactttt tgggccaaat cgccaggcaa
       61 aaaccccagt ttttcacccg cttccaccgc agggcgcacc aacacgatgc ggtctaccaa
      121 gtggcgctcc attgcatcca ccgccgccgc taccgccaaa tacgtcttgc ccgtaccagc
      181 agggcctaag ccaaacacca catcgtggtt gtgtaaggct ttgatgtagc cattttggcg
      241 cggggtgcgg cctgtaagat tgccacggcg ggtgcgaaag ctgtattgct gtttttcatt
      301 ataaggctgc tcgcgttcgc gctcacgaaa ttcatgttgc acttgctcgc tcacttgttg
      361 cgccttggcg gccaccacag acaattggat gtcggtgtcg gtcaattcgc gctgctgtgc
      421 caaacccagc aattgttgta aacaagccat ggccactgcg gcatcttcac ctttgatgag
      481 caaggcatcg gccgcacggc ggatttccac attcaagctt ttgcttaagg catcaaaatt
      541 cgcgcccaat ggcccagaca ggcgttgtag ggtcaattgg tcttgattgt ccaatgcaaa
      601 agtcattgtg ttgctcatag agattccttg gttagagctt gcattttatc acagccttgg
      661 gccgcgactg cattgtgtta aaggcgggtg gaggcgatgg ctagtgcaat actagtgcaa
      721 taaatggtga attgtcggcg gttgcttgcg catttcaagg tagaatctgg gttcaaaaca
      781 gactctaagc ggcctgtaaa tggctgaaaa cagacttaca ggccgcttat aaacccagaa
      841 cgatggatgt tatgcaaagc aaaatactca ttttgatgtg tgcggtgtta ttgggattgg
      901 cggcatgtgg caaaggcgac aatgcggcga aatcaccaca aacccctgcg gccaagcaat
      961 tgtctgcagc cgatgtgctc accatgcagg cgcaagattt tgcccaagtg gtggcgttta
     1021 caggctcgct taagccaata aaagaagcga ctgtgagcgc ggagtcgggc ggtacggtcg
     1081 cgaagttgct ggtagatgag ggcaatgcgg tgcaagcagg gcaagtgatt gcggtgatgg
     1141 acaaccaagc ggtgcgtgaa agcctgcaag gccagcaagc gcaagtgcaa aacagccaag
     1201 ccaatctcga tttggccgcc accaaactca aacagcaaca agtgttgttc ggcaaaggct
     1261 ttgtgtccaa gctggctttg gagcaagcgc agaacgagta caaagtggct ttgggcagcc
     1321 acaaagcgca acaagcagaa ttgtccaagc tgaacaaaaa ccaagccgat gccaatgtgc
     1381 gcgcgcccat cagtgggggg gtgtatgaca aactggtcaa tgcgggcgaa ttggtgaatg
     1441 cgggcgggca gattttgaaa attgctcaaa ccaatgtgtt ggaaatcgcc gccaccgtgt
     1501 cgtctgagca agtgacgttt gtgaaagagg ggcaagaggt gaaattcact gtcgccacag
     1561 gcgcgcccga gcacgcaggg cgcattgtgc gcatcaatcc cgttgccgat gccaataccc
     1621 gccaattcac tgtgttcata caagtacaaa acgccgatgg ggcggtgaaa gcagggcagt
     1681 ttgctcaagg caatatcgtg ctcaatcaag tggcgcaagc gatggtggtg cctgaaacgg
     1741 cgattaaaca cgccgaagga caggcatttg tgtatgtggt gagccaaggc aagctggcca
     1801 agcggcctgt aaaaattgtg ttgcaagacc caagcactgc ccgcgcggca gtagaaggct
     1861 tg
//
LOCUS       DAOMVK010000453         1850 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_18420_length_1850_cov_2.115320, whole genome shotgun sequence.
ACCESSION   DAOMVK010000453 DAOMVK010000000
VERSION     DAOMVK010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18420_length_1850_cov_2.115320"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>476
                     /locus_tag="PK856_11485"
     CDS             <1..>476
                     /locus_tag="PK856_11485"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRL03031.1"
                     /translation="NPQESQRQHKLQQLPQANFSSWQQQHPGLHSLLPDAAGLWWPQG
                     AWVSPPLWIKAMLQHRLITVHEHSIVTHLQAAENTWQVHTATGDIHSASHVIVCNGAA
                     ADTFSQTSHLQLRRIRGQVSYATATASSSHLSAAISAAHYITPSFQNRHTFGASFVL"
     assembly_gap    477..486
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <487..908
                     /locus_tag="PK856_11490"
     CDS             <487..908
                     /locus_tag="PK856_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRL03032.1"
                     /translation="DSTNVRESEHQHNLVGLAEIQPDLAKEFGLQQPSGRASVRADSI
                     DHLPVVGPVGKADEMRALYAQLSLDRNFHLQAACPFWEGLWVNVAHGTRGMLTAPLSA
                     YHLTKQILEQKSVLSQSLQDNLHPNRLLIRQLIYANA"
     gene            981..>1850
                     /locus_tag="PK856_11495"
     CDS             981..>1850
                     /locus_tag="PK856_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRL03033.1"
                     /translation="MNVPMSDNNMPKADACRSDGFLSERISDKAEHVFARSSSAQLTT
                     DNEITCLLDSRENFPAWLSAMEQAKYTICIEMYIFATDEFGQKVRSVLLGRLQQGVQV
                     FLIYDWVGSSGAHFKRFFKPLITAGAQVQAYNKLGLTTGLSFLGRNHRKSFTIDQKVA
                     FVSGLCISSQWGGNEKHGISPWRDTGLMLQGPIVQDVLNAFLDTWQAMGLTKPEVLLQ
                     VAPSDAATVVDDYADQLTDQNDANSNKPKSFANARLVATTADNANMMRLDLLAVSMAR
                     KTLWITDAYFMPTR"
BASE COUNT          492 a          508 c          417 g          423 t
ORIGIN      
        1 aacccacaag aaagccaacg ccaacacaaa ttacagcaac tgccacaagc gaacttctca
       61 agctggcaac agcaacaccc aggtttgcat tcgctcttac cagatgcggc tggtttatgg
      121 tggccacaag gtgcctgggt cagcccaccc ttgtggatca aagccatgtt gcaacaccgc
      181 ttgattaccg tacacgaaca ctcaatagtc acacatttac aggccgctga aaatacatgg
      241 caagtgcaca cggccacagg agacatacac tcagcttccc atgtcattgt gtgcaatggc
      301 gcggcggcag atactttcag ccaaacctca cacttgcaac tgcggcgcat ccgtggccaa
      361 gtgtcctatg caacagccac agccagcagc tcacatttga gcgcggccat cagcgcggcc
      421 cactacatca ccccctcttt ccaaaatcga cacactttcg gcgccagttt tgtgctnnnn
      481 nnnnnnatga cagcacaaac gttagagaat ccgaacacca acacaatctt gtgggtctgg
      541 ctgaaataca gccagatttg gccaaggaat ttggcctaca acagccctca ggccgtgcct
      601 cggtacgcgc agacagcata gaccacttgc ctgtggttgg ccctgtgggc aaggcagatg
      661 aaatgcgcgc cctttatgcc caactcagtt tggatcgcaa tttccattta caggccgcat
      721 gtcctttttg ggagggtttg tgggtaaatg tcgctcatgg tacccgtggc atgctcaccg
      781 cacccctgtc tgcgtaccac ctgacgaaac aaattctaga gcaaaaatcc gttttatctc
      841 aatcacttca agacaattta caccccaatc gcttgttaat acgccaatta atttatgcaa
      901 atgcctagca taatgctatg agaatcaggg cttgcgaggt atgatgaact caagtcatca
      961 attgtcagga tgttttgagt atgaacgtgc ccatgtcaga caacaacatg cccaaggcag
     1021 atgcttgccg ctcagatggt tttctgtctg agcgcatcag tgacaaggcc gaacatgttt
     1081 ttgcacgctc atccagcgct caactcacca ccgacaatga aatcacctgt ctgttagaca
     1141 gtcgcgaaaa tttccccgcc tggctcagtg cgatggaaca agccaaatac accatatgca
     1201 ttgaaatgta cattttcgcc accgatgaat ttggccaaaa agtacgcagc gtgttgctag
     1261 gccgcttgca acaaggcgtg caagtctttt tgatttacga ctgggtaggc agcagcggtg
     1321 ctcacttcaa acgctttttc aagcctctga ttacagcagg cgctcaagtt caagcctata
     1381 acaaactggg tttgacaaca ggcttgtctt ttttaggcag gaatcaccgc aaatctttca
     1441 ccatagacca aaaagtcgcg tttgtgagcg gcttgtgcat ctcctcacaa tggggaggca
     1501 atgaaaaaca tggcatatcg ccttggcgcg acactggctt gatgttgcaa ggcccaatag
     1561 tacaagatgt tttgaatgct tttttggaca cttggcaagc aatgggctta accaagcccg
     1621 aagtcttgtt acaggtcgca ccgagcgatg ctgcgaccgt agtcgatgac tatgcagacc
     1681 aactcaccga ccaaaacgat gccaacagca acaaacctaa gagctttgcc aatgcgcgct
     1741 tggtcgccac aacagcagac aacgccaaca tgatgcgctt ggatttgttg gccgtgagca
     1801 tggcccgcaa gactttgtgg attaccgatg cctatttcat gcccacacgc
//
LOCUS       DAOMVK010000454         1849 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_18427_length_1849_cov_2.551839, whole genome shotgun sequence.
ACCESSION   DAOMVK010000454 DAOMVK010000000
VERSION     DAOMVK010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18427_length_1849_cov_2.551839"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..740)
                     /locus_tag="PK856_11500"
     CDS             complement(54..740)
                     /locus_tag="PK856_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRL03034.1"
                     /translation="MTPERADILISSFWPMVVSTAQFTIPLALASFVIGLLIAVLTAV
                     VRVMPRDAWWKKVLYAIARVYVSMIRGTPMLVQIFIIFYGLPAVGVTLDPFPTAIIAF
                     ALNVGAYASEIIRAAIESVEKGQWEAGYSGGMTYFQVLRYIVLPQAMPVSVPPLSNTF
                     ISLVKDTSLASLVLVTELFRVAQAVAARTYEFLLVYAEAALIYWIVCLVLGLGQERLE
                     RYFNRYRNRY"
     gene            complement(763..1614)
                     /locus_tag="PK856_11505"
     CDS             complement(763..1614)
                     /locus_tag="PK856_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HRL03035.1"
                     /translation="MIKTLPLTLITGLILSACGGQSGSTAAPADNAASGATPVTQTTE
                     TPAGGSLADRIASKGTIVVGTEGTYAPFTFRNEKGELTGYDVDVMNEVGKRLGVKVEY
                     KETQWDAIFAGLNSNRFDLIANQVGVNDERKAKYEFSQPYTYSRPVVVTRADDASITK
                     FEDIKGKKTAQSLTSNYGKMAEKYGADITGVEGLAQAIELLKQKRIDLTLNDELAILD
                     FLKTKGDAGLKIALRGDDVETMAFVFNKGNDKAIAEINKQLDAMHQDGTFAKISTKYF
                     GKDVSVK"
     gene            complement(1663..>1849)
                     /locus_tag="PK856_11510"
     CDS             complement(1663..>1849)
                     /locus_tag="PK856_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03036.1"
                     /translation="TAIISLRASWKAICLSMPNTWVCKRAVLVKDWFDDVSNCAEHMP
                     KKHFKYVRAWFSIASCK"
BASE COUNT          484 a          486 c          423 g          456 t
ORIGIN      
        1 tccagttcac acagcaaatt ttgtgcttac aggacgcttt aaaaggtttc cattcagtag
       61 cggttgcgat agcggttaaa ataccgctcc aaacgctctt ggcccaagcc caataccaaa
      121 cacacgatcc agtaaatcaa agccgcttca gcatacacca acaaaaattc atatgtgcga
      181 gccgccaccg cttgtgccac ccgaaacaac tcggttacca ataccaacga tgccaaagac
      241 gtgtctttga ccaaggaaat aaaggtattg gacagcggtg gcaccgacac aggcatggct
      301 tgtggcaaca cgatgtagcg caagacttgg aaatacgtca tcccgcctga atagcccgcc
      361 tcccattggc ctttttccac cgattcaatc gccgcacgaa tgatttcaga agcgtaagcc
      421 cccacattca gagcaaaggc aatgatggcg gtgggaaacg gatccaaagt caccccaacc
      481 gcaggcaaac cataaaagat gatgaaaatc tgcaccaaca tcggcgtgcc acgaatcatg
      541 gacacgtaca cgcgggcaat ggcatacagc acttttttcc accaagcatc gcgcggcatc
      601 acccgcacca cagcggttaa cacagcgatg agcaagccga tgacaaacga ggccaaggcc
      661 aatggaatcg tgaattgcgc ggtggacacc accataggcc aaaacgagct gatgagaata
      721 tcagcccgct ctggggtcat gaagggcagg tttgccagca aattacttaa cgctgacatc
      781 tttaccaaag tatttggttg aaatcttggc gaaagtgccg tcttggtgca tggcgtctaa
      841 ttgcttgttg atttcagcaa tcgctttgtc attgcctttg ttgaacacaa atgccatggt
      901 ttccacatcg tcaccgcgca aggcaatttt caagcctgca tcgcctttgg ttttcaagaa
      961 gtccaaaatc gccaattcgt cgtttaaggt caaatcgatg cgtttttgtt tcaacaactc
     1021 aatcgcttgt gccaaacctt caacgccagt gatgtctgcg ccgtattttt ccgccatttt
     1081 gccgtagtta ctggtcaaag attgggcggt tttcttgcct ttgatgtctt caaatttggt
     1141 gatagaagca tcgtcagcac gggttaccac cacgggacgt gagtaagtgt aaggttgtga
     1201 gaattcgtat ttggctttgc gctcgtcgtt cacacccact tggttggcaa tcaagtcaaa
     1261 gcggttgctg ttcaaacctg caaaaatcgc atcccattgg gtttctttgt attccacttt
     1321 tacgcccaag cgtttgccga cttcgttcat tacgtctacg tcgtagccag tcaattcacc
     1381 tttttcattg cggaaggtga acggcgcata agtgccttca gtacccacca cgatggtgcc
     1441 tttgctggca atgcggtcgg ccaagctgcc acccgcaggt gtttcagtcg tttgggtgac
     1501 aggggtagca ccagacgcag cattgtccgc aggcgctgct gtgctgccac tttggccgcc
     1561 gcacgcgctc aaaatcaaac cagtaatcaa ggtcaaaggg agggttttta tcatgttcat
     1621 gttgtcttct cttaatgcgt gttgggttca aacagcgcgg tctcatttac aggacgctat
     1681 actaaaccat gcgcgaacat acttgaaatg cttttttggc atatgctcag cacaattgct
     1741 tacatcatca aaccaatcct taacaagcac cgcccgctta caaacccaag tgttcggcat
     1801 ggaaagacag atggctttcc acgaagctcg caatgaaata atagctgtg
//
LOCUS       DAOMVK010000455         1849 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_18429_length_1849_cov_2.409142, whole genome shotgun sequence.
ACCESSION   DAOMVK010000455 DAOMVK010000000
VERSION     DAOMVK010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18429_length_1849_cov_2.409142"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..176
                     /locus_tag="PK856_11515"
     CDS             <1..176
                     /locus_tag="PK856_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HRL03037.1"
                     /translation="IIHGSGLGSRGFAPVLKNTVRRWLTLHPDVLAYTEPHGSNDGAV
                     LILLKRVWKEENE"
     gene            642..>1849
                     /gene="aceE"
                     /locus_tag="PK856_11520"
     CDS             642..>1849
                     /gene="aceE"
                     /locus_tag="PK856_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285327.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HRL03038.1"
                     /translation="MAEQFKDIDPIETQEWLDSLASVLQSEGPERAHYILQRLVSEVR
                     RTGVHLPFSATTAYINTIPVGKEQKSPGNHELEHRLRSIIRWNAAAVVLRGGDKNEEL
                     GGHIASYQSAATLYDVGFNHFWKASSEDEDGDLVFIQGHSAPGIYSRAFLEGRLTEAQ
                     LDNFRQEVDGHGLPSYPHPWLLPDFWQFPTVSMGLGPLMGIYQARFLKYLESRGLART
                     KGRKVWVFCGDGEMDEPESLGAISLAAREGLDNLVFVINCNLQRLDGPVRGNGKIIQE
                     LEGDFRGSGWNVLKVIWGGRWDPLLAQDKDNLLKQRMAECLDGDYQTFKSKNGAYVRE
                     NFFGAYPELKAMVANWSDEDIWALNRGGHDPHKVYAAYHEATTNANGRPTVILAKTTV
                     GRPLALVVAS"
BASE COUNT          474 a          385 c          474 g          516 t
ORIGIN      
        1 aaatcattca tggcagtggc ttgggttcgc gagggtttgc cccagtttta aagaatacgg
       61 tgcggcgctg gttgacgcta caccctgatg tgctggctta cacagagccg catggcagca
      121 atgatggtgc ggtgctgatt ttgctcaaac gggtttggaa agaggaaaac gaataatgtg
      181 aagaaatccg cacaaaagag tacagtgtat ttgtcctaaa acagcggcct gtaaaccaca
      241 ggtcgcttgt tttttgcaaa ttagatattt tatattattc atatggaaat aatttatagt
      301 tatttcattt tgttcgtttt ctttctgatg cagacaaaaa atcgaattgc gctggtgtac
      361 attaacaaag ccatatgatg agaaatgact gacggaacaa ttgctcacac tgagatttgc
      421 tgatacgata attccgaaaa tacagtatgt tgcggcgcag caaagtgtgt agatgcctga
      481 atgtcttcag tcagttgcct caaaatcgcc aatgcattgt attccatttt ctgcaaatta
      541 gttgaacgca aacgaatcaa gcttgtttca aaaacgaaaa gtttgggtag ttttgtttaa
      601 taatgttgtt ttatgaacat ttgagtgctg aaggataaac tatggctgag caattcaaag
      661 acattgatcc aattgaaacc caagaatggc tggattcatt ggcgtcggtt ttacaaagtg
      721 aaggacctga gcgtgcgcat tacattttgc aacgcttggt gagtgaagta cgccgcacag
      781 gtgtgcacct gcctttcagc gccacgactg cctacatcaa caccattccc gtgggcaaag
      841 aacaaaaatc gcctggtaac catgaattag aacaccgttt gcgttccatc atccgctgga
      901 atgccgctgc cgtggtgttg cgcggtggtg ataaaaacga agaattgggt ggccacattg
      961 cttcttacca atccgcagcc actttgtacg atgtgggctt taaccatttc tggaaagcca
     1021 gcagtgaaga tgaagatggc gatttggtgt tcatccaagg ccactctgcg cctggtattt
     1081 attcgcgcgc cttcttggaa ggccgtttga ctgaagcgca gttggacaat ttccgccaag
     1141 aggtagatgg tcacggcttg ccttcatatc ctcacccttg gttgttgccc gatttctggc
     1201 aattcccaac tgtgtctatg ggcttgggtc cattgatggg catttaccaa gcccgtttct
     1261 tgaagtattt ggaatctcgt ggtttggcac gcaccaaagg ccgtaaggtt tgggtgttct
     1321 gtggtgacgg tgaaatggat gagcctgaga gcttgggcgc aatttcattg gcggcccgtg
     1381 aaggcttgga caatttggtg ttcgtcatca actgtaactt gcaacgcttg gatggccctg
     1441 tgcgtggtaa cggcaaaatc attcaagaat tggaaggtga cttccgtggt tcgggctgga
     1501 atgtgttgaa agtgatttgg ggtggtcgtt gggatccttt gttggctcaa gacaaggata
     1561 acttgctcaa acaacgcatg gccgaatgtt tggacggcga ttaccaaacc ttcaaatcca
     1621 aaaatggcgc ttatgtgcgt gaaaacttct tcggcgccta ccctgaattg aaagccatgg
     1681 ttgccaattg gtctgatgaa gacatttggg ccttgaaccg tggtggtcac gacccacaca
     1741 aagtgtatgc ggcttaccat gaagcgacaa ccaacgccaa tggtcgtcca actgtgattt
     1801 tggccaaaac cacagttgga cgaccattgg cgttggttgt cgcttcatg
//
LOCUS       DAOMVK010000456         1848 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_18442_length_1848_cov_3.233129, whole genome shotgun sequence.
ACCESSION   DAOMVK010000456 DAOMVK010000000
VERSION     DAOMVK010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18442_length_1848_cov_3.233129"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="PK856_11525"
     CDS             complement(<1..754)
                     /locus_tag="PK856_11525"
                     /inference="COORDINATES: protein motif:HMM:NF025460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4272 domain-containing protein"
                     /protein_id="HRL03039.1"
                     /translation="MTLLVNAYATVVVLPELNFRGEVLNQRDLSDPELAEHLQGFIGY
                     VLQLDGEEMTQAKYYTMKHIERVKHQVSAEVDEDDLDDFVAWAERANAIVYLPDGSVR
                     APNGAQILPATDELILPPTLTSAWARKQRTEAVLAQLNIRTPASLPAVIADEELSLRP
                     ASVCLQRALGCCVAAVMADTRASGDPFSVTELQERFPQAYGWMTSDERDFVENANASE
                     RDCVNFTWRYEAVATLLWALNALPSLPDADEVC"
     gene            complement(888..1631)
                     /gene="fabG"
                     /locus_tag="PK856_11530"
     CDS             complement(888..1631)
                     /gene="fabG"
                     /locus_tag="PK856_11530"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HRL03040.1"
                     /translation="MDLGLQGKVALVTGASRGIGAAIADSLAAQGAIVIGTATSEAQA
                     AKINERLSAHGGAGRVLNVGEVESIETLIADITKEFGTIEILVNNAGVTRDNLLMRMK
                     DEEWDDIMHINLKSVYRASKAVLRGMMKARNGRIISIASVVGAMGNAGQANYAAAKAG
                     MMGFTKSLAREVGSRGITVNCVAPGFIDTDMTRALPEEQRAALAGNIALGKLGEAQDI
                     ADAVVFLASNQAKYITGQTLHVNGGMLMP"
     gene            complement(1647..>1848)
                     /locus_tag="PK856_11535"
     CDS             complement(1647..>1848)
                     /locus_tag="PK856_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009426320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="malonyl CoA-acyl carrier protein transacylase"
                     /protein_id="HRL03041.1"
                     /translation="LVRQLYSPVLWTQTVNKLVEDGITEAAECGPGKVLAGLSKRINP
                     EASCVALTKQEDLEAFIAAHSA"
BASE COUNT          421 a          558 c          438 g          431 t
ORIGIN      
        1 cgcacacctc atctgcatca ggcaagcttg gcaaggcatt cagcgcccac agcaaggttg
       61 cgactgcctc ataacgccaa gtgaaattga cacagtctcg ctcgctcgca ttcgcatttt
      121 cgacaaaatc acgttcatca gacgtcatcc agccataggc ttgtggaaag cgctcttgca
      181 actccgtcac gctgaatggg tcgccactgg cgcgggtatc ggccatcacc gccgccacac
      241 aacagcccaa agcacgttgc aaacacacag acgcagggcg caacgacaat tcttcgtctg
      301 caatcacagc aggcaaagac gcaggcgtgc gaatgtttag ctgcgccaat accgcttctg
      361 tgcgctgttt gcgcgcccaa gcactggtca aagtcggtgg taaaatcagc tcatcggtgg
      421 cgggcaaaat ttgcgcacca ttgggcgctc gcaccgagcc atcaggcaga tacacaatcg
      481 cattggcacg ctcagcccaa gccacaaaat catccaaatc gtcttcgtcc acctcagcac
      541 tgacttggtg tttcacccgt tcgatgtgct tcatggtgta atacttggct tgcgtcatct
      601 cctcgccatc gagctgcaac acatagccga tgaagccttg caaatgctcg gccagctcag
      661 ggtcgctcaa atcgcgttga ttcaacacct cgccccgaaa attcaattca ggcaatacca
      721 ccaccgttgc atacgcattc accaacagag tcatttccca cctcgcctat ttggatgttt
      781 tgggcatggt aacagagata aagcataggc tctagcgagt atctgtttgc agcacaaaaa
      841 agcggcctgt aagcttacag gccgctttca aacacttttc aaactgctta aggcatcaac
      901 atgccgccat tcacatgcaa ggtttggcct gtgatgtatt tggcttggtt agacgccaaa
      961 aacaccaccg catcggcgat gtcttgcgct tcgcccaatt tgcccaaggc aatgttgccc
     1021 gctaaagcgg cgcgttgctc ttctggcaag gcgcgggtca tgtcggtgtc gatgaagcca
     1081 ggggccacac agttcactgt gatgccacgg ctgcccactt cgcgcgccaa tgatttggtg
     1141 aagcccatca tgcctgcttt ggcagccgcg taattggctt ggcctgcgtt gcccatcgcg
     1201 cccaccacag aggcgatgct gatgatgcgg ccattgcgcg ctttcatcat gccacgcaac
     1261 acggctttgc tggcgcggta aaccgatttc aagttgatgt gcatgatgtc gtcccactct
     1321 tcgtctttca tgcgcatcaa caaattgtcg cgggtcacgc ctgcgttgtt caccaaaatt
     1381 tcgatggtgc caaattcttt ggtgatgtcg gcgatgaggg tttcaatcga ctctacttca
     1441 cccacattca acacgcggcc tgcgccacca tgcgcactca aacgctcatt gattttcgcc
     1501 gcttgcgctt ccgacgtggc cgtaccgatg acaatcgcgc cctgcgctgc caagctgtcg
     1561 gcaattgccg caccaatacc gcgcgatgcg ccagtgacca aagccacttt accttgtaat
     1621 cccaaatcca tgttcatccc tttagattaa gcgctgtgag cagcgatgaa agcttccaag
     1681 tcttcttgtt tggtcaaggc cacacaagaa gcttctgggt taatgcgttt ggacaagcct
     1741 gccaacacct tgcctgggcc acattctgcc gcttcggtga tgccgtcttc taccaattta
     1801 ttgaccgttt gtgtccacaa cacgggtgaa tacaattggc gcaccaac
//
LOCUS       DAOMVK010000457         1845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_18484_length_1845_cov_2.846927, whole genome shotgun sequence.
ACCESSION   DAOMVK010000457 DAOMVK010000000
VERSION     DAOMVK010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18484_length_1845_cov_2.846927"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PK856_11540"
     CDS             complement(<1..238)
                     /locus_tag="PK856_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HRL03042.1"
                     /translation="MNDYKLLSALLSYPSADLQAAIDSELLPILESQPDWQDKLMPLL
                     RYLADTDLIEVQERYVATFDRNPNHALHLFEHLHG"
     gene            complement(238..1779)
                     /gene="narH"
                     /locus_tag="PK856_11545"
     CDS             complement(238..1779)
                     /gene="narH"
                     /locus_tag="PK856_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009116352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HRL03043.1"
                     /translation="MKIRAQIGMVLNLDKCIGCHTCSITCKNVWTSRDGVEYAWFNNV
                     ETKPGVGYPKQWENQDIHKGGWVRKSNGKLELKQGSKLKILANIFANPNMPSIDQYYE
                     PFTYDYEHLQNAPEMPTPPTARPVSVLTGKKMDKIHWGPNWEDDLGGEFQQRAKDELF
                     EGIQKDMYSTFENTFMMYLPRLCEHCLNPACVASCPSGSIYKREEDGIVLIDQDKCRG
                     WRMCVSGCPYKKIYYNWTSGKAEKCTFCYPRIEAGQPTLCSESCVGRIRYLGVLLYDA
                     DKIEQAAAVGNEQDLYQSQLDVFLDPNDPLVQAEALKQGIPQSWIDAAQNSPVYRMAV
                     EWKVAFPLHPEYRTLPMVWYIPPLSPIQSAVESGMIGKHGLIPEVKDLRIPLQYLANL
                     LTAGKEEPILNALETMLTMRRYMRQKNVPQTNVTFEEILDKTHLTGAQIEEMYQIMAI
                     ANYEDRFVVPTSHKEYAENAFGEKGACGFTFGNGCSDGTSEQSLFGQRKASPVVFHGM
                     RKGAK"
BASE COUNT          430 a          445 c          441 g          529 t
ORIGIN      
        1 caccatgcaa gtgctcaaat aaatgcaaag cgtgattcgg gttgcgatca aatgtggcca
       61 cataacgttc ttgcacctca atcaagtcgg tgtcggcgag atagcgcagc aatggcatca
      121 atttgtcttg ccaatcaggt tggctttcca atattggcaa caattcgctg tcaatagcgg
      181 cctgtaaatc tgcactgggg taggacaaca atgcagacag caatttgtag tcattcatta
      241 tttcgctcct ttgcgcatac catggaacac cacgggtgag gctttgcgtt gtccaaacag
      301 gctttgttca gaagtgccat ctgagcaacc attgccaaaa gtgaagccac aagccccttt
      361 ttcgccaaac gcattctcgg catattcctt gtgcgaagtg ggcaccacaa aacggtcttc
      421 atagttggca attgccatga tttggtacat ttcttcaatc tgagcaccag tcaaatgggt
      481 tttgtccaaa atttcctcaa acgtcacatt ggtttgcggc acattttttt ggcgcatgta
      541 gcggcgcatg gtcaacatgg tttccagtgc attcaagatg ggttcttctt tgcctgccgt
      601 gagcaagttg gccaagtatt gcaatggaat gcgcaaatct ttcacttctg gaatcaaacc
      661 atgtttgcct atcatgccgc tttccactgc cgattgaatc ggtgacaagg gtgggatgta
      721 ccacaccatc ggtaaggtgc ggtattcagg gtgtaagggg aaggccactt tccactcaac
      781 tgccatgcgg taaacaggtg agttttgcgc agcatctatc catgattgag ggatgccttg
      841 cttcaaagcc tcggcctgta ccaatggatc gttcggatcc aaaaacacgt ctaattgtga
      901 ttggtacaaa tcttgttcat tgcccacagc agctgcttgc tcgattttgt cggcatcgta
      961 caaaagcaca cccaagtagc gaatgcggcc tacacagctt tcagaacaca gagtcggttg
     1021 tcctgcttca atgcgtgggt agcagaacgt gcatttttca gctttgccac tggtccagtt
     1081 gtagtaaatc tttttgtaag ggcagcctga cacacacatg cgccagccac ggcatttgtc
     1141 ttggtctatc aaaacaatgc catcttcttc acgcttgtaa atcgagccag atgggcaaga
     1201 agccacacag gctggattca ggcagtgctc acacagacgt ggcaaataca tcatgaacgt
     1261 gttctcaaac gttgagtaca tgtctttttg tatgccttcg aacaactcgt ctttggcgcg
     1321 ttgttggaat tcacctccca aatcatcttc ccaattcggg ccccagtgga ttttgtccat
     1381 tttcttaccg gttaacaccg atacaggccg agccgttggg ggggtaggca tttcgggggc
     1441 attttgcaaa tgctcatagt cgtaagtaaa cggctcgtag tattggtcta tgctgggcat
     1501 attgggattg gcaaagatgt tggccaaaat tttcaatttg ctgccttgct tcaattccaa
     1561 tttgccattg gatttgcgta cccagccacc tttgtggatg tcttggtttt cccattgctt
     1621 ggggtagccc acacctggtt tggtttccac attgttgaac caggcgtatt ccacaccatc
     1681 acgactggtc cacacgtttt tacaggtgat ggagcaggta tggcagccta tgcatttgtc
     1741 gaggttcagc accatgccga tttgtgctct gattttcata ttgtgttcct tatcgtgacc
     1801 ttaggcttct tgttggctgg gcgcgttaac aggggtgtcc atcca
//
LOCUS       DAOMVK010000458         1843 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_18513_length_1843_cov_1.869687, whole genome shotgun sequence.
ACCESSION   DAOMVK010000458 DAOMVK010000000
VERSION     DAOMVK010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18513_length_1843_cov_1.869687"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="PK856_11550"
     CDS             <1..665
                     /locus_tag="PK856_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRL03044.1"
                     /translation="KERASKGLPAVKTIPPGPKNPLGPVFVRFGDPKLGLGIHGTSAP
                     SSVPSFASHGCVRLRSENALEFAKYIDKGSRVSVIYNESALNLDASNNLWLSAYKDPY
                     NLKKMNPAAVKAQAQSIAQKYGKTVNEKMLTQVLANRSGQLNCLTCVKGSGKVQGALR
                     PVSMTFKTPPPPPATANPTPLPSSAPITEPGDNGSDYIKPHNPVDWSSKLETRDSFAR
                     LW"
     gene            complement(758..>928)
                     /locus_tag="PK856_11555"
     CDS             complement(758..>928)
                     /locus_tag="PK856_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HRL03045.1"
                     /translation="KSCKTAVFANKLLDHEKPIVADYVGLDVPNRYVFGYGMDVSGAW
                     RNLSAIYALKGH"
     assembly_gap    929..938
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<939..1319)
                     /locus_tag="PK856_11560"
     CDS             complement(<939..1319)
                     /locus_tag="PK856_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRL03046.1"
                     /translation="MSTRNREETQAILDTADLLFSEAQCDEALERMAAEITQDMSDSY
                     PLVLPVMGGAVVFTGKLLPLLRFPLDFDYVHVSRYGDKLEGGNFSWTRAPQDKSIAGR
                     DVLILDDILDEGQTMRVIKEQVMAM"
     gene            complement(1593..>1843)
                     /locus_tag="PK856_11565"
     CDS             complement(1593..>1843)
                     /locus_tag="PK856_11565"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0006399 - tRNA metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HRL03047.1"
                     /translation="DVGGAVLLVSQFTLYGDARNGRRPSYTEAARPEMATHAYELVAK
                     LLRAHGLTVATGEFQTHMQVSLCNDGPVTLLLDSHKQF"
BASE COUNT          460 a          478 c          484 g          411 t
ORIGIN      
        1 aaaaagagcg cgcgagcaaa ggcttgcctg cggtgaaaac catcccacct ggccctaaaa
       61 acccattggg tcctgtgttt gtgcgctttg gcgatcctaa gctgggcttg ggcatacacg
      121 gcaccagcgc gccgtctagc gtgccgagtt ttgccagcca tggctgtgtg cgtttgcgca
      181 gtgaaaacgc gttggaattt gccaaataca tcgacaaagg ctcgcgtgtg tcggtgattt
      241 acaatgagtc ggctttgaat ttggatgcca gcaacaattt gtggttgtcg gcttacaaag
      301 acccgtacaa tttgaaaaaa atgaaccctg ccgcggtcaa agcacaagcg caaagcattg
      361 cccaaaaata cggcaaaacc gtgaatgaga aaatgctgac gcaagtgttg gccaaccgtt
      421 ctgggcagtt gaattgcctg acctgtgtga aaggctcggg caaagtgcaa ggtgcgttgc
      481 gccctgtgag catgactttc aaaacaccgc caccaccgcc tgccacggcc aatccgaccc
      541 cattgccttc gtctgcgccg attaccgagc ctggtgacaa tggttcggat tacatcaagc
      601 cacacaatcc cgtggattgg agcagcaaat tggaaacccg tgattcgttt gcccgtttgt
      661 ggtgatgcga tgagcacaaa aaacaggatg cctaagcatc ctgtttttta ttgggcagtg
      721 tttacaggcc gcatgcggcc tgtaaatcat gatgagccta gtggcctttt aaagcgtaaa
      781 tcgcagacag gttgcgccaa gcgccgctca cgtccatgcc gtaaccaaac acatagcgat
      841 tgggcacgtc taagcccacg taatcggcca caatcggttt ttcgtgatcg agcaatttgt
      901 tggcgaatac ggcggttttg caagatttnn nnnnnnnnca tggccatcac ctgttctttg
      961 atgacgcgca tggtttgccc ttcgtccaaa atatcatcca aaatcaacac atcacggcca
     1021 gcaatgcttt tgtcttgtgg cgcgcgcgtc cagctgaaat tgccgccttc caatttgtcg
     1081 ccatagcgag aaacgtgcac gtaatcgaaa tccaaaggga agcgcaacaa aggcaataat
     1141 ttgccggtga acaccaccgc accgcccatc acaggcaaga ccaatgggta agaatcgctc
     1201 atgtcttggg tgatttcagc ggccatgcgc tccaatgctt catcgcattg ggcttcgctg
     1261 aacaacaaat cagcggtatc gagtatggct tgggtttctt cgcggttgcg tgttgacatg
     1321 gtgtgatgct gatttggtaa aacggtgata atgggctcat tataccgagc tatcagaaca
     1381 gatgaaatca aacagcgatt gaaaaaaagt atcaatcgca ctctagagat gtcgcgcgct
     1441 gtatttgcac tgagcgcacg ggttgggaat agggtcacgc ccgccattgc cgcgggcaaa
     1501 cgccaaggtg cagcccatca ttatcaccgt tttaccaaat cagcatcagc gaatggccaa
     1561 caagggttta caggccgcat gtggcgggcg tgttaaaact gtttgtggct gtcgagcagc
     1621 aaggtcacgg gcccatcgtt gcacaaactc acttgcatgt gggtttgaaa ctcacccgtg
     1681 gccacggtta agccatgcgc gcgcaacagc ttggccacca attcgtaagc atgggtggcc
     1741 atctcagggc gggccgcttc ggtgtaagag gggcgacggc cattgcgggc atcgccatac
     1801 aaagtgaatt gcgacaccaa caataccgcg ccgcccacat cca
//
LOCUS       DAOMVK010000459         1842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_18529_length_1842_cov_2.010632, whole genome shotgun sequence.
ACCESSION   DAOMVK010000459 DAOMVK010000000
VERSION     DAOMVK010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18529_length_1842_cov_2.010632"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="PK856_11570"
     CDS             complement(<1..535)
                     /locus_tag="PK856_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HRL03048.1"
                     /translation="MDFLQSRLKFRQRTPWEAVDLGRSLINSRLTYYMALYAVLMLPF
                     VLIGVAGAWLFDLRGWLLAWIWWLKPVYEYGVLSALSIHAFQPLPPLKIAIKTGFKLM
                     VRSCVLGDLTWRRLRPMRSFVLPIQQLEQLSGSDFQTRKRELGRYGGNTATGLTLMGN
                     ALEGIWLYALMILAYWFL"
     gene            complement(539..>1355)
                     /locus_tag="PK856_11575"
     CDS             complement(539..>1355)
                     /locus_tag="PK856_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003822554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HRL03049.1"
                     /translation="CRSLQNLVDRAHRQLYQRKNSIRKQFSQFVKYTLPQTFRQEKAV
                     IVWAHLLFYAPLFICLLLTWRHPDFLDSIAGRGVGESMGDGYSEMAELQAKGASRPWG
                     QNWMMFGFYIFNNISIGFQSFVGGLVFGLGAMYVAVYNGLMIGSVMGYMTTHPAGPVF
                     FSFVGAHGSFELTGIVLSVAGGLKLGYSLINPGAYNRRDALRIQGKQASALICGAFLF
                     LFIAAIIEGFWSPITVLPLWLKYTVAVILWLSVYAYLFLAGKQAPADALNRER"
     assembly_gap    1356..1365
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1518..>1842)
                     /locus_tag="PK856_11580"
     CDS             complement(1518..>1842)
                     /locus_tag="PK856_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRL03050.1"
                     /translation="MLLTAKGQRIGDIFAGTIVVDEHTERQIGRLAYLEHIPSVTPPI
                     MLSREEQYSIVSFTERHPRLPRVRQHELAEQLCYDLQMPSENPVETVLGMGKYYLGQQ
                     QKDAA"
BASE COUNT          486 a          461 c          479 g          406 t
ORIGIN      
        1 acaaaaacca atacgccaaa atcatcaagg catagagcca aatgccttct aaagcattgc
       61 ccattaaggt taaacctgtg gcggtgttgc cgccatagcg acccaattca cgcttgcggg
      121 tttgaaaatc actgccagac agttgttcca attgctgaat cggcaaaaca aagctgcgca
      181 tggggcgcag gcgtcgccag gttaaatcac ccaacacaca cgagcgtacc atcagtttaa
      241 aaccggtttt gatggcgatt ttcagaggtg gtaagggctg aaacgcatgt atcgacaagg
      301 ccgataacac gccgtattca tacacaggct ttagccacca aatccatgcc aagagccagc
      361 cacgcaaatc aaacagccat gcaccggcca cgccaatgag gacaaaaggc aacatcaaaa
      421 cggcatacaa agccatgtaa taggtgaggc gtgaattgat taagctgcga cccaaatcca
      481 ccgcctccca aggggtgcgt tggcgaaatt tcaggcgaga ttgcagaaag tccatggctt
      541 agcgctcccg attcaatgca tcggcagggg cttgtttgcc agccaaaaac agataagcat
      601 acacagacag ccacaaaatc acggccacag tgtatttgag ccataatggc aacacggtga
      661 tgggcgacca aaagccttcg atgatggcgg caatgaacaa aaacaagaat gcgccacaga
      721 ttaaggccga ggcttgtttg ccttgtatgc gcaaggcatc acggcggttg tatgcgccag
      781 ggttaattaa gctgtaaccc agtttcaggc cgcctgctac cgataacaca atgccagtca
      841 attcaaatga gccgtgagcg cccacgaatg agaaaaagac ggggccagct gggtgggtag
      901 tcatgtagcc catcaccgag cctatcatca ggccattgta aaccgcgaca tacatcgcac
      961 ccaagccaaa caccaagccg ccgacaaagc tttggaagcc gatgctgatg ttgttgaaaa
     1021 tgtaaaaacc aaacatcatc cagttttgtc cccatggacg gctcgcacct ttggcctgta
     1081 actctgccat ttcgctgtag ccatcgccca tggattcgcc cacgccgcgt ccggcaatgc
     1141 tgtctaaaaa gtcaggatga cgccaagtca gcaacaagca aatgaataaa ggtgcgtaaa
     1201 acaacaaatg ggcccacaca atcaccgctt tttcttggcg gaaggtttgt ggcaaggtgt
     1261 atttcacgaa ttggctgaat tgtttgcgga tggagttttt gcgttggtag agctgcctgt
     1321 gcgcgcggtc taccaggttt tggaggctgc ggcacnnnnn nnnnnggcca aggccaaatg
     1381 gtggcacaag gtgcggtatt gcgggccaaa ctctgccgtg cgctggttgt tgagttggcg
     1441 cttttgttcc agtttgttca aatcttgttc aaactgctgc caaaattctt ggtgtaaggc
     1501 ctcgaatgcg tgttgtctca tgccgcatcc ttttgttgtt gacccaaata gtatttgccc
     1561 atgcccaaaa cggtttcgac tgggttttca gatggcattt gcaagtcgta acacaattgt
     1621 tctgccaatt cgtgttggcg cacacgcggt aaacgcgggt ggcgctcggt gaaagaaacg
     1681 atggagtatt gttcttcgcg cgagagcatg atgggcgggg tgacgctggg tatgtgttcc
     1741 aaataggcca agcggccaat ttggcgctcg gtgtgttcgt ctaccacgat ggtgcccgcg
     1801 aaaatgtcgc ctatgcgttg gcctttggcg gtcagcaaca tg
//
LOCUS       DAOMVK010000460         1839 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_18565_length_1839_cov_5.047085, whole genome shotgun sequence.
ACCESSION   DAOMVK010000460 DAOMVK010000000
VERSION     DAOMVK010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18565_length_1839_cov_5.047085"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..1301)
                     /gene="purD"
                     /locus_tag="PK856_11585"
     CDS             complement(36..1301)
                     /gene="purD"
                     /locus_tag="PK856_11585"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRL03051.1"
                     /translation="MKVLVIGSGGREHALAWRLAQSARVDEVLVAPGNAGTATEPKLT
                     NVNVSAHRDLMQLARDQDVAFTLVGPEAPLAAGIVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMAKYNIPTAGYQTFSDAAEAHAYIDAKGAPIVIKADGLAAGKGVIVAMSDD
                     EAHAAIDDMLVGNKMGDAGARVVIEDFLTGEEASFIVMVDGEHVLAMATSQDHKRLLD
                     GDQGPNTGGMGAYSPAPVVTDAVYQRVMDEIIYPTVRGMQAEGHPFSGFLYAGLMIDE
                     QGAPFTIEFNCRFGDPETQPIMSRLESDFSELVEAGIDGRLHKVEAKWSTQSAVGVVL
                     AAAGYPESPKKGDVITGTDAANQHGKVFHAGSKLNEQGELVTNGGRVLCVVGLGDDVK
                     QAQTQAYAALEHIQFDGMQYRRDIANKAL"
     gene            1451..>1839
                     /locus_tag="PK856_11590"
     CDS             1451..>1839
                     /locus_tag="PK856_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03052.1"
                     /translation="MKKLALSLLLIGVLSACQPQKEEAATVACDSDVVKSTFDLGTKQ
                     QIMAKLKYLAQAQPEVYGSLNFSQIEQALDSIQFNLESIHTESKPTDTPQMCQADVVV
                     SIPNDVVIAAQLKANELNQTFNLAAEA"
BASE COUNT          442 a          511 c          427 g          459 t
ORIGIN      
        1 gtttcacatg aaacacttac aggccgctta ctccattaca aagccttgtt ggcgatgtca
       61 cggcggtatt gcataccgtc aaattgaatg tgttccaaag ccgcataagc ttgggtttgc
      121 gcctgtttca catcgtcgcc caaacccacc acacacaaga cgcggccacc attggtgacc
      181 aattcacctt gttcattgag tttgctgcct gcgtggaaca ctttgccatg ttggttggca
      241 gcgtctgtgc ctgtgatcac atcgcctttt ttcggcgact caggataacc cgctgctgcc
      301 aacaccacgc ccaccgccga ttgcgtactc cacttggctt ccactttgtg caaacggcca
      361 tcgatgcccg cttccaccaa ttctgagaaa tccgattcca aacggctcat gatgggctga
      421 gtttcaggat cgccaaagcg gcaattgaat tcaatcgtaa acggcgcgcc ttgctcatct
      481 atcatcaagc ctgcgtacaa aaaccctgaa aatggatggc cttcggcttg catgccacgt
      541 acggttgggt aaatgatttc gtccatcacg cgttggtaaa ccgcatcagt caccacgggt
      601 gcagggctgt aagcgcccat gccgcctgtg ttcgggcctt ggtcgccatc gagcaagcgt
      661 ttgtggtctt ggctggtggc catggccaac acatgctcgc catccaccat cacgatgaag
      721 ctggcttctt cgccagttaa aaagtcttca atcaccacac gtgcgcccgc gtcgcccatt
      781 ttattgccca ccaacatgtc gtcgatggcg gcgtgggctt catcatcgct catggccaca
      841 atcacgcctt tgcctgcggc caaaccatca gctttaatca caatcggtgc gcctttggca
      901 tctatgtaag catgcgcttc tgcggcatcg ctgaaggttt gatagcctgc ggtgggaatg
      961 ttgtatttgg ccatgaaggc tttggcaaag tcttttgagc tttccaattg cgccgcgtac
     1021 tgagtggggc caaatatttt caagcctgct gcgcgaaaat catcgacgat gcctgccgcc
     1081 aacggcgctt ctgggcccac caaggtaaac gccacatctt ggtcacgtgc caattgcatc
     1141 aagtcacggt gagcagacac attcacgttg gtcaatttcg gctcagtcgc agtacctgca
     1201 ttgcctggcg ccaccaacac ttcatcaacg cgcgcagatt gcgccaaacg ccatgccaat
     1261 gcgtgctcac gcccgccaga accaattacc aaaactttca tctgtctgcc caatgatgga
     1321 atcgaaaggc gttattttaa tgcgctgtcg taagcttgcc tagttaaatg ccaaagtacg
     1381 caaaaataaa acctctaatc gaaattattg gcgataatga ttttttctat tttcagtggg
     1441 aatccgttgc atgaaaaaac tcgctttatc acttttgctg attggggttt tgagcgcttg
     1501 ccagccacaa aaagaggaag ctgccaccgt tgcttgtgac agcgatgtgg tgaaatccac
     1561 gtttgatttg ggtaccaaac agcaaatcat ggcgaagttg aaatacttgg cacaagccca
     1621 accagaagtg tatggcagtt tgaatttcag ccaaattgaa caggctttgg acagcatcca
     1681 attcaaccta gaatccattc acaccgaatc caaacccacc gacacgccgc aaatgtgtca
     1741 ggccgatgtg gtggtttcca ttcccaatga tgtggtgatt gcggcgcaat tgaaagccaa
     1801 tgaattgaac caaaccttca atttggccgc cgaagcctt
//
LOCUS       DAOMVK010000461         1839 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_18567_length_1839_cov_2.580157, whole genome shotgun sequence.
ACCESSION   DAOMVK010000461 DAOMVK010000000
VERSION     DAOMVK010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18567_length_1839_cov_2.580157"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PK856_11595"
     CDS             complement(<1..214)
                     /locus_tag="PK856_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HRL03053.1"
                     /translation="MSTASSTPRQNRSWLGKPSMSSAIRFGVWIVSIVYVVLYVIHSG
                     LLDFFLTEGTQNVVFVIALVAALVGAY"
     gene            complement(264..>765)
                     /gene="ung"
                     /locus_tag="PK856_11600"
     CDS             complement(264..>765)
                     /gene="ung"
                     /locus_tag="PK856_11600"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRL03054.1"
                     /translation="KVVILGQDPYHGPEQAHGLAFSVRTGVKIPPSLRNMYKELSSDI
                     EGFQIPSHGNLNHWAEQGVLLLNTVLTVEAGQAHSHAKWGWERYTDKVVDVINQHQQN
                     VVFLLWGSHAQKKGAMIDRSRHLVLEAVHPSPLSAHRGFFGCQHFSLTNQYLEETGQA
                     PIHWQV"
     assembly_gap    766..795
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1062..1511
                     /locus_tag="PK856_11605"
     CDS             1062..1511
                     /locus_tag="PK856_11605"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HRL03055.1"
                     /translation="MKFTTRPPFELTGLHIHTTWQNNQAKTDIARLWKRFYKDDIAGQ
                     LDFSVDDTVYLLYTDYHDSSNQGFSVWLGLETEIDAPLPMDMRRFKIPALNYYQFEAS
                     SEMPEDMLALWETVHQAEAQLKRSYRCDFETYPDGENAQLFISTSHE"
     gene            1587..>1839
                     /locus_tag="PK856_11610"
     CDS             1587..>1839
                     /locus_tag="PK856_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HRL03056.1"
                     /translation="MLPLHVPVIIVGAGPVGMLLSLQLHQQGQRVLLLEARPKTVPPQ
                     DKRTLALSYNSVMAFEQAGVDLSQAHMTAIHQVHISQQGA"
BASE COUNT          479 a          492 c          412 g          426 t
ORIGIN      
        1 cgtatgcgcc caccaaggcc gccactaagg caatcacaaa caccacgttt tgcgtgccct
       61 cggtcaggaa aaagtccaat aagccgctgt gtatcacata caaaaccacg tacacaatgg
      121 acacgatcca cacgccaaaa cggatggcgc tggacatcga cggtttaccc aaccaactgc
      181 ggttttgtct gggtgtggaa gaagctgtac tcatggtgga ttccgtttta taagcggcct
      241 gtaagcttta caggccgcat ggtttatacc tgccaatgga tgggcgcttg ccctgtttct
      301 tctaaatact gattggtcag agaaaaatgt tggcaaccaa aaaagccacg gtgcgccgac
      361 aaaggcgacg ggtgcaccgc ctctaaaacc aaatggcgcg aacggtctat catcgcgcct
      421 tttttctggg catggctgcc ccacaataaa aacaccacgt tttgctggtg ttggttgatg
      481 acatcgacca ctttgtcggt gtatcgttcc cagccccatt tggcatgcga atgggcttgt
      541 cctgcctcca cggtcaaaac cgtgttcaaa agcaacacgc cttgctcggc ccaatggttc
      601 aaattgccat ggctgggtat ttgaaaaccc tcaatatcgc tgctcaactc tttgtacata
      661 ttgcgcagcg aaggcggtat tttcacccca gtgcgcaccg aaaaagccaa gccatgcgct
      721 tgctcagggc catggtaagg gtcttgcccc aaaatcacca ctttgnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnaaaag cctgaagcac ctcagcctcg ggcggataca ccgttttccc
      841 atcggcccgc tcttgctgaa ctttgtggat taacgcttga aaataaggct gctgttgctc
      901 agcagacaag gcatcttgcc aatttaagac gtttgcttgc atctgttcct gaataaccca
      961 tctgaatgat gattaaattt tacaataagc ggcctgaact tgtgcccctc tgtcagcatc
     1021 gtatgcacat agaatgactt gctcgcacag gaagcctgca catgaaattc accactcgcc
     1081 ctccatttga attgactgga ttgcacatcc acaccacttg gcaaaacaac caagccaaaa
     1141 ccgatattgc cagattgtgg aagcgcttct acaaagacga catcgctggt caattggatt
     1201 tcagtgtgga cgacacagta tacctactct ataccgatta tcacgattct agcaaccaag
     1261 gtttcagtgt gtggttggga ttggaaaccg aaatcgacgc gcctttgcct atggacatgc
     1321 ggcgattcaa aatacctgca ctcaattatt atcaatttga agccagctca gaaatgcccg
     1381 aagacatgtt ggctctttgg gaaacggttc accaagccga ggcccaatta aaacgcagtt
     1441 accgctgtga ttttgaaacc tatcccgatg gtgaaaatgc ccaattgttc atttccacca
     1501 gtcacgaata agcaaaccaa gctcatcccc tccgacagcc gcgccaaatc aggtacaatt
     1561 cgggcataaa cagtaaagga tggtttatgt taccgctgca tgtaccggtg attatcgtgg
     1621 gcgcagggcc agttggcatg ttgctcagcc tgcaattaca ccaacaagga caacgtgtct
     1681 tgcttttgga agcgcgcccc aaaaccgtgc ccccgcaaga caagcgcacc ttggcgttgt
     1741 cgtataacag cgtgatggct tttgaacaag cgggcgtaga cttgagccaa gcgcacatga
     1801 ccgccatcca ccaagtacac atttcccaac aaggcgcgt
//
LOCUS       DAOMVK010000462         1836 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_18603_length_1836_cov_2.098259, whole genome shotgun sequence.
ACCESSION   DAOMVK010000462 DAOMVK010000000
VERSION     DAOMVK010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18603_length_1836_cov_2.098259"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PK856_11615"
     CDS             complement(<1..234)
                     /locus_tag="PK856_11615"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRL03057.1"
                     /translation="MFGIENYWGFIVAGIVLAVTPGTDTIYILSRTLAQGKGAGIAST
                     LGVSSGVLIWGLLVSFGLATVLTQLPSVLLFIKI"
     gene            complement(222..797)
                     /locus_tag="PK856_11620"
     CDS             complement(222..797)
                     /locus_tag="PK856_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HRL03058.1"
                     /translation="MTYCQFAATLAADSTDPNKHYHDHVYGFAVGADNELFARLVLEI
                     NQAGLSWTTILKKWQGFYQAYAKFDIATVAAFGDADVARLLADAGIIRNRLKIQAAVY
                     NAQQILVLQQEYGSFKAWLDHHRPLSLEAWVKLFKQHFKFVGGEIVNEFLMSTGYLAG
                     AHAADCPVLTSVLASQPAFVNTQSKDKPCLE"
     gene            complement(892..1191)
                     /locus_tag="PK856_11625"
     CDS             complement(892..1191)
                     /locus_tag="PK856_11625"
                     /inference="COORDINATES: protein motif:HMM:NF017198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HRL03059.1"
                     /translation="MQKAVRDRLEQIPVTEIYLGICWFGLISSIVLLAVGLWNAAMPG
                     TEKGFYGMGYLLSLFAAVAVQKNVRDTLQFRNENGIDSATHSGSFLQRRFGKDEE"
     gene            complement(1517..>1836)
                     /gene="gcvH"
                     /locus_tag="PK856_11630"
     CDS             complement(1517..>1836)
                     /gene="gcvH"
                     /locus_tag="PK856_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRL03060.1"
                     /translation="GSVTVGITDHAQELLGDIVFVELPEVGANLAADEQAGVVESVKA
                     ASDIYAPIAGEILEVNQELIDAPDTANTDPYGAAWFFRIKPANAADLDGLMDAAAYEA
                     ETN"
BASE COUNT          528 a          488 c          377 g          443 t
ORIGIN      
        1 gattttgata aacagcaata ccgaaggcaa ttgtgttaac accgtggcca agccaaacga
       61 caccaacaaa ccccaaatca acacaccact gctcacgccc aaggtcgacg caatgcctgc
      121 gcctttgcct tgcgccaagg tgcggctcaa aatgtaaatc gtgtcagtgc caggtgtgac
      181 cgccagcacg atgcccgcca caataaagcc ccaataattt tctattccaa acatggcttg
      241 tccttgcttt gtgtgttcac aaacgcaggc tgactggcca ataccgatgt caacacggga
      301 caatcggcag catgggcacc ggccaaatat ccggtactca tcaaaaattc gttgacgatt
      361 tcgccaccaa caaatttaaa gtgttgctta aacagcttta cccacgcctc taaactcaaa
      421 gggcgatggt ggtctagcca agctttgaaa gagccgtatt cttgttgcaa caccaaaatt
      481 tgctgcgcgt tgtaaacagc ggcctgtatt tttaaacggt tgcgaatgat gcctgcatct
      541 gccagcaatc gcgccacatc ggcatcgcca aacgcagcca ccgtggcaat gtcaaacttg
      601 gcataggctt ggtaaaagcc ttgccatttt tttaagatgg tcgtccagct caatcctgct
      661 tggttgattt ccaacaccaa gcgcgcaaac aattcattgt ctgcccccac tgcaaaacca
      721 tacacatggt catggtaatg cttgttagga tcggtgctgt cagcagccaa ggtcgctgca
      781 aattggcaat atgtcattgc gctttaacca tcaaaaataa aaccggtcac agccatgctg
      841 tcaccggtaa gaaactgggg gagattcgat tattcagtca taagtagcag gttattcctc
      901 atctttgccg aatcgccgtt gtaaaaacga tcctgaatga gttgctgagt ctatgccatt
      961 ttcgtttcga aattgcaaag tgtcgcggac atttttttgc accgctaccg cagcaaacag
     1021 gctgagcaaa tagcccatcc cataaaaacc tttttccgtg cccggcatgg cggcattcca
     1081 caaacccact gccaacaaca cgatggaact tatcaaacca aaccaacaaa tgcccaaata
     1141 aatttcggtt accggtattt gttcgagtcg atctcgcact gctttttgca aggaaacggc
     1201 tgcaaacaaa ccaaataata gcaatactaa ataatagcaa tactaaataa tagccttttt
     1261 cactcaaggc caattgtgca ttccacaggc ctagcaaata ggtaatggtg ccaacagcca
     1321 aagctgtcca cgatgcacca acaaaagcac gactgggttc ttgtaaagcc atttgatcac
     1381 tcccttacag gtttgccgaa taggtaaaac catcatgcaa cactttgctg taagtcggtg
     1441 ataattcagt gtaagtagca agtattcatc tactttagtg caacacttgc tacttcacgt
     1501 gtcatgcaag caagacttag ttggtttctg cttcgtaagc tgcagcatcc atcaagccat
     1561 ccaaatcagc tgcgttagct ggtttgatgc ggaagaacca agccgcgccg taaggatcgg
     1621 tgttggcagt gtcaggagcg tcaatcaact cttggttcac ttccaaaatt tcacccgcga
     1681 ttggcgcgta aatgtcagaa gccgctttca cagattcaac cacaccagct tgctcatcag
     1741 cagccaagtt agcgcccact tctggcaact caacaaacac gatgtcaccc aacaactctt
     1801 gggcgtgatc ggtaatacca acggtcacag aaccat
//
LOCUS       DAOMVK010000463         1835 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_18620_length_1835_cov_3.192135, whole genome shotgun sequence.
ACCESSION   DAOMVK010000463 DAOMVK010000000
VERSION     DAOMVK010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18620_length_1835_cov_3.192135"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..482
                     /locus_tag="PK856_11635"
     CDS             159..482
                     /locus_tag="PK856_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03061.1"
                     /translation="MWMVFVAGLVVLGILSLYVEALAQDWQRWAEKRPEWATQVPYFG
                     PVSSASHYPYKHARLWWDMCRHRAWQQQYDLPEDAYQHWLLVGGFYFSLTTLEVLTLL
                     WWWMQ"
     gene            complement(569..1273)
                     /locus_tag="PK856_11640"
     CDS             complement(569..1273)
                     /locus_tag="PK856_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRL03062.1"
                     /translation="MSATLYLIPTPLATADTPCLLPHEQALMVGITDFIVEAEKTARA
                     HLKALGVTTPIRELSMHTLNEHTDAADIPALLEPLKQGRDVGLVSEAGCPAVADPGAQ
                     VVALAHQYGFTVKPLVGPSSILLALMASGANGQNFAFRGYLPQAADERVAALKDWEAR
                     AYQQKETQIFIETPYRNDVLLETALQTLKPTTRLCVACDLTADTETIVSLPVQDWPKE
                     RPNLKKRPTIFIIYAQ"
     gene            complement(1270..>1835)
                     /locus_tag="PK856_11645"
     CDS             complement(1270..>1835)
                     /locus_tag="PK856_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HRL03063.1"
                     /translation="ASSSRFRQMQLQQLNLPFTTCSPDIDETPLADESAAATALRLAT
                     GKAQAVAENHPQHLIIGSDQVAFCQQTQLGKPMSVAKAHDILRFTSGQHLDFYTAVVL
                     LNTLTGRMQSHVDHTQVQMRELSDASIARYLEREPDAIYCAGGAKSEGLGQTLIASIT
                     STDPNALIGLPMLSLIGFLINEGHSLP"
BASE COUNT          389 a          416 c          566 g          464 t
ORIGIN      
        1 gcggcaaggt ggccgatgcc atcgcttcgg gtgtgaagcg gtatttcgcc agcggcgcag
       61 tgttggcaca gcgctaacag tgaatatcaa gcggcctgta aacatgtttt aaacatttac
      121 aggccgcttt tttatgggga aatgcttaag gagacagtat gtggatggtg ttcgtagcgg
      181 gcttggtggt gttgggtatc ttgagtttgt acgtcgaagc cttggcgcaa gactggcagc
      241 gttgggcaga aaagcggcct gagtgggcga cgcaagtgcc gtattttggc cctgtgtcgt
      301 cagcctcaca ttatccttac aagcatgccc gcttgtggtg ggacatgtgc cgccaccgag
      361 catggcaaca gcaatacgat ttgcctgaag acgcttatca acattggttg ttggtgggtg
      421 gcttttactt tagtttgacc acattagagg tgttgacctt attgtggtgg tggatgcagt
      481 aatccaatac tcgaagcggc ctgtaaatat ttaaaacatg cttacaggcc gcttttgatt
      541 gccaatcttt tacatcacag gcaaatgctt attgtgcgta aatgatgaaa atggtggggc
      601 gttttttcaa gttcggacgt tcttttggcc agtcttgtac gggcaaagac acgatggttt
      661 cggtgtcggc ggtcaagtca caggccacac acaagcgcgt ggtcggcttg agtgtttgca
      721 aagcggtttc caataacaca tcgttgcggt aaggcgtttc gatgaagatt tgggtttctt
      781 tttgttggta ggcgcgtgct tcccaatctt tcaaggcggc gacgcgctcg tcagcggctt
      841 ggggcaaata gccgcggaag gcgaagtttt gcccgttggc acctgaggcc atcaaagcca
      901 ataaaatgct tgatgggccg accaaaggtt tgaccgtaaa gccatattgg tgcgccaaag
      961 ccaccacttg cgcgcctggg tcggccacgg cagggcaacc cgcttcgctg accaagccca
     1021 cgtcacggcc ttgttttaaa ggctcaagca aggcaggaat gtcggcagcg tcggtgtgct
     1081 cgttcaaagt gtgcatggac aattcgcgta tcggcgtggt cacgcccaag gcttttaaat
     1141 gcgcgcgcgc ggttttttcg gcttcgacga tgaaatcggt aatgcccacc atcaaagcct
     1201 gctcatgtgg caacaggcaa ggcgtgtcgg cggtggcaag tggggtgggg atgaggtaga
     1261 gggtggcgct catggtaagc tgtgtccttc gttgattaaa aagccaatca ggctgagcat
     1321 gggcaagcca attaaggcat tggggtcggt gctggtgatg gaggcaatca gggtttggcc
     1381 caagccttcg cttttggcac cgcctgcgca gtaaatcgca tcgggctcgc gctcaagata
     1441 gcgggctatg cttgcatcgc ttaattcgcg catttgcact tgggtgtggt cgacatggct
     1501 ttgcatgcgg cctgtaagcg tgttgagcaa gaccacggcg gtgtaaaaat ccaaatgttg
     1561 gccgctggtg aagcgcaaaa tgtcgtgggc tttggccacg ctcatcggtt tgcccaattg
     1621 cgtttgttgg caaaacgcca cttgatcgga gccaataatc aaatgctggg gatggttttc
     1681 agccacggct tgggctttgc ctgtggccaa acgcaaggcg gtggcggcgg ccgattcgtc
     1741 tgcaagcggc gtttcgtcta tgtctggcga gcaagtggtg aagggcagat tcaattgttg
     1801 caattgcatt tggcgaaagc gtgaactcga tgcca
//
LOCUS       DAOMVK010000464         1829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_18714_length_1829_cov_1.621759, whole genome shotgun sequence.
ACCESSION   DAOMVK010000464 DAOMVK010000000
VERSION     DAOMVK010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18714_length_1829_cov_1.621759"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..782
                     /locus_tag="PK856_11650"
     CDS             96..782
                     /locus_tag="PK856_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HRL03064.1"
                     /translation="MSESKLELLNPHNCQVIFIDQQPQMAFGVQSIDRQTLKNNVVGL
                     AKAAKIFNIPTTITTVETESFSGHTFPELLDVFPDAPLLERTSMNSWDDQKVRDALAN
                     NQRKKIVVSGLWTEVCNLTFSLSCLHDTDYEIYMVTDASGGTSQEAHDMAIQRMIQVG
                     VIPVTWQQVLLEWQRDWAHRDTYDAVMALVKEHSGAYGMGVDYCYTMVHKAPERTQSK
                     HEILAPVAAK"
     gene            884..>1829
                     /locus_tag="PK856_11655"
     CDS             884..>1829
                     /locus_tag="PK856_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRL03065.1"
                     /translation="MAHVQLILKSGKITTLNKKQPEVQALAIADGKILRAGSNEEVMK
                     LAQADTKIVDLNGRRVIPGLNDSHLHLIRGGLNYNMELRWEGVPSLSDAMRLLKEQAD
                     NTPAPQWVRVIGGWSEFQFAEKRLPTLDEINKAAPDTPVFVLHLYASAMLNRAALKAL
                     GFDRNTPNPPGGQIQRDAHGEPTGLLLATPSAMILSSTLGMAPKLELEDQINSTRHYM
                     RELNRLGITSAIDAGGGGQNYPDDYAVIQRLHADQHLNIRIAYNLFAQKAGSEYESYQ
                     EWTQMTFPGDGDDYLQMNGAGENLTWSAGDFENFLEPRP"
BASE COUNT          480 a          447 c          453 g          449 t
ORIGIN      
        1 gagtcaaccc ttcactttgc tacccatgtc ttttgcaacg gtggtgcagt taaactgctt
       61 gattccaaac acccttaatc catgaaggaa atcacgtgtc tgagtccaaa ttagaattgt
      121 taaaccctca caattgtcag gtgattttca ttgaccaaca gccacaaatg gcttttggtg
      181 tgcaatccat agaccgccaa actttgaaaa acaatgtggt gggtttggcc aaagccgcga
      241 aaattttcaa cattcccacc accatcacca cggtagaaac cgaaagtttc tctggccata
      301 ctttccctga attattggat gtcttccctg atgcgccttt gcttgagcgc acgtccatga
      361 attcttggga cgatcaaaaa gtacgcgacg ccttggccaa caaccaacgc aaaaaaattg
      421 tggtgtcagg cttgtggaca gaagtgtgta atttgacgtt ttcattgagc tgtttgcacg
      481 ataccgatta cgaaatctac atggtgaccg atgcctcagg cggcacttcg caagaggcgc
      541 acgacatggc gatacagcgc atgatacaag tgggcgtgat acccgtgact tggcagcaag
      601 tgttgttgga atggcaacgt gattgggccc atcgcgacac ttacgatgcg gtaatggctt
      661 tggtaaaaga gcattctggt gcgtatggca tgggcgtgga ttattgctat accatggtac
      721 ataaagcccc tgagcgcacc caaagcaagc acgaaatttt ggcacctgtg gccgcgaagt
      781 aaacgtgatg atgaggcagt taccttaaag ggtagctgcc tttttgtttt gcccattgcc
      841 tattcattaa tagaatttca catatcttaa agtgaggtaa accatggccc acgttcaatt
      901 gatactcaaa agcggcaaaa tcaccacctt aaacaaaaag caaccggaag ttcaggcctt
      961 ggccattgcc gatggcaaaa tcctacgtgc tggcagcaat gaagaggtga tgaaattggc
     1021 gcaagccgac accaaaatcg tcgatttgaa tggccgccgt gtgatccccg gcttgaatga
     1081 cagccatttg catttgatac gaggcggcct gaattacaat atggaattgc gttgggaagg
     1141 tgtgccttct ttgagcgatg ccatgcgttt gctcaaagag caagccgaca acacgcctgc
     1201 gccgcaatgg gtacgggtga taggtggctg gtcagaattt cagtttgctg aaaaacgctt
     1261 gcccacctta gatgaaatca acaaggccgc gcctgatacc cctgtgtttg tgctgcattt
     1321 gtacgccagc gccatgctca atcgcgccgc attaaaagcc ttgggttttg atagaaacac
     1381 gcccaatcct ccaggtggcc agatacagcg cgatgcccac ggtgagccta cgggtttgtt
     1441 attggccacg ccttcggcca tgattttgtc ttccactttg ggcatggcgc cgaaattgga
     1501 gttggaagac caaatcaatt ccacccgcca ctatatgcgc gagcttaacc gcttgggcat
     1561 cacctcggcc attgatgcgg gcggtggtgg ccaaaactac cccgatgact atgcggtgat
     1621 acagcgcttg catgccgacc aacacctcaa tattcgcatt gcctacaatt tgtttgcaca
     1681 aaaagccggt tcagaatatg aaagctacca agagtggacg caaatgacgt ttcctggcga
     1741 tggcgatgac tatttgcaaa tgaatggcgc gggagaaaac ctcacgtggt cggccggaga
     1801 ttttgagaat ttcttagagc cacgccctg
//
LOCUS       DAOMVK010000465         1823 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_18787_length_1823_cov_2.205317, whole genome shotgun sequence.
ACCESSION   DAOMVK010000465 DAOMVK010000000
VERSION     DAOMVK010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18787_length_1823_cov_2.205317"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..>656)
                     /locus_tag="PK856_11660"
     CDS             complement(168..>656)
                     /locus_tag="PK856_11660"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRL03066.1"
                     /translation="APDFFRDNINFAIVWGKQGDWPHLATVPIIAGNLTPVCSPAYLQ
                     EHGEIKVAADLNQHILIHEENHVGWQQWLYKATGQEMQAGKGFHIDDTNVRMHAVLNG
                     QGIMLGCPALLQPLLEQGELVQLFEAYLDEYAYFLAYPKEANLSRQEQDFLDWVASIA
                     LT"
     assembly_gap    657..668
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<669..1054)
                     /locus_tag="PK856_11665"
     CDS             complement(<669..1054)
                     /locus_tag="PK856_11665"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRL03067.1"
                     /translation="MQVPLLNALKTFTVSAQRLNFTKASEDLLVSPSAISHQIKILEE
                     YLGCKLFLRKNRSLELTEEGLFLFNRLQRPFYEINQALSELRMPHGKSRINLSLRPFV
                     SSMWFTKQLGDLWRKHPGIEINLIHS"
     gene            complement(1267..1342)
                     /locus_tag="PK856_11670"
     tRNA            complement(1267..1342)
                     /locus_tag="PK856_11670"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1307..1309),aa:Ala,seq:ggc)
     gene            complement(1660..>1823)
                     /locus_tag="PK856_11675"
     CDS             complement(1660..>1823)
                     /locus_tag="PK856_11675"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HRL03068.1"
                     /translation="HRIVDRSHGMVREEVRCSTCDAHLGHVFPDGPPPTGERYCINSL
                     SMTFVNTND"
BASE COUNT          528 a          433 c          398 g          452 t
ORIGIN      
        1 ggacaccact taatgggctt atcgttagaa gtgagcccat catgcggcag aagcgtgggc
       61 ttgaaaattg aggaaatgtg aacgatgctt gagttttttt aaacgtaaaa aagccgcttg
      121 tatgtctgca agcggcctgt aaatgtctta atgcgtctga attggcctca agttaaggcg
      181 atggaagcca cccagtctaa aaaatcttgc tcttggcgac tgagattggc ctctttgggg
      241 taagccaaaa aatacgcata ctcatccaaa taggcctcaa acagctgcac caattcacct
      301 tgctccaata aaggctgcaa taaggctggg cagcccaaca taatgccttg cccattcaac
      361 accgcgtgca tgcgcacatt ggtgtcatcg atgtgaaagc ctttgccagc ttgcatctct
      421 tgccccgtcg ccttatacaa ccattgctgc cagcccacat ggttttcttc gtggattaaa
      481 atgtgttgat tcaaatcggc ggccaccttg atttctccat gctcttgcaa gtaagcaggt
      541 gagcacacag gggttaaatt gcccgcaata atcggcaccg tggccaaatg cggccaatcg
      601 ccttgcttgc cccacacaat ggcaaaattg atgttatcgc gaaaaaagtc aggggcnnnn
      661 nnnnnnnnaa actgtgtatc aagttgattt caatgccagg atgtttgcgc cacaaatcgc
      721 ccaattgctt ggtaaaccac atgctcgata caaatgggcg cagcgataaa ttgatgcgac
      781 tcttaccatg tggcatgcgc aattcactta aggcttggtt gatttcataa aacggacgtt
      841 gcaaacggtt gaataaaaac aagccctctt ccgttaactc aagcgagcgg tttttgcgca
      901 aaaacaattt acaccccaaa tactcttcca agattttgat ttgatggctg atggccgatg
      961 gcgacaccaa caagtcttcc gacgccttgg taaaattcaa acgctgtgct gaaacagtaa
     1021 aggtttttaa agcatttaat agcggtactt gcatgctgca tcctgagtaa aaagcacaaa
     1081 aaattcacag ccacgccatg aaatcacgct ttaaaataca atgaataccg cacacgaagc
     1141 aagccaccga cttggattac aatcttaatg aagtgagtga agatgcccta cagaaaggcc
     1201 ttgatgcgca cccaaacagg ccgccatatg aaaaaagcct gacttaaagt caggctttta
     1261 gaattttggt ggaggtaagc gggatcgaac cgctgacctc ttgcatgcca tgcaagcgct
     1321 ctaccaactg agctataccc ccaaaagaga tgtgcatatt atcagcattt ttctgatttg
     1381 caaacatgac gaaggcgaaa ttgagcgaaa ttctcaccca tttgaaataa acatttgatg
     1441 ctaaaaaaca ttttttcgca cgctactcgt cgaaatcgcc caacatttgc ttcgcctttt
     1501 gtaaagtgaa gtttgtcgac ttaacaaaca gcctttattt ttgtgagatg cgtagacctc
     1561 aagccaatgt gcacacttga ttgagtacaa tccacaaata catgcacaag cggcctgtaa
     1621 acttgaaaac agtgtttaca ggccgcttat ttagcaccat caatcgtttg tgttcacaaa
     1681 cgtcatggac aaagaattga tgcaatagcg ctcacctgtg ggcggcgggc catcggggaa
     1741 aacatggcct aaatgggcat cgcatgtgct gcagcgcact tcttcgcgca ccatgccgtg
     1801 actgcggtca acaatgcggt ggg
//
LOCUS       DAOMVK010000466         1822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_18795_length_1822_cov_2.745331, whole genome shotgun sequence.
ACCESSION   DAOMVK010000466 DAOMVK010000000
VERSION     DAOMVK010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18795_length_1822_cov_2.745331"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="PK856_11680"
     CDS             <1..538
                     /locus_tag="PK856_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HRL03069.1"
                     /translation="GQPNVDAQALASSLNISSDESLAWQAHVTCLLNSVPNSNGVLLT
                     GPTGEANSLNWHMPSSVLIDGGDEVSVAALAAVLKALKVNVHLSPNHAASARLERIGV
                     RALTEAQLQTVPLEVCVSLGTCDSGLKSSLASRKGTLVKLVDLSAGLDLAPLLHEISR
                     STNTAAAGGNAALMTLQQ"
     gene            717..998
                     /locus_tag="PK856_11685"
     CDS             717..998
                     /locus_tag="PK856_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03070.1"
                     /translation="MDEQEFKLYELYLRIQTLESSVAAILWAISQQATNSDAVVATVL
                     AQIQTSTAMTLEHLQALGFSEEQLQRLQATALGVQDVYEAMKLNHEQVS"
     gene            complement(1071..1661)
                     /gene="azu"
                     /locus_tag="PK856_11690"
     CDS             complement(1071..1661)
                     /gene="azu"
                     /locus_tag="PK856_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009118765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HRL03071.1"
                     /translation="MSHVQHLFWLKLSLGLTLGLGLSACGGEQAIAPSNTAASAAAPV
                     ASSPNTASEAANSAPVAASAPTAATAECSTDLSANDAMQYSSKEIAIPASCQQFTVHF
                     KHAGSMPKAAMGHNVVVAKTSDLNGVIKDGMKAGPAADYVKAGDERVLAKTKLLGGGE
                     SDSLTLDVAAIKAAPYSFVCTFPGHTGSMRGTIVIK"
BASE COUNT          420 a          421 c          524 g          457 t
ORIGIN      
        1 ggggcagccc aatgtggatg cgcaagcctt ggccagcagc ttgaacatca gcagcgatga
       61 atctttggca tggcaagctc acgtcacgtg tttgctcaac agcgtgccca acagcaatgg
      121 ggtgttgctc acaggcccaa caggcgaagc gaacagcctc aattggcata tgcctagcag
      181 cgtgttaatc gatggcggcg acgaagtcag cgtggcggct ttggcggcgg tgttgaaagc
      241 cttgaaagtg aacgtgcatt taagcccgaa tcatgcggca tcggcccgtt tggagcgcat
      301 aggcgtgcgc gcgctcaccg aagcgcaatt acaaaccgtg cctttggaag tgtgcgtgag
      361 cttgggcact tgtgacagtg gcttgaaatc cagtttggcc agccgcaaag gcacgctggt
      421 gaagctggtg gatttgagcg caggcttgga tttagcgcct ttgttacacg aaatcagccg
      481 cagtaccaac acggcagcgg caggtggcaa tgcggcgttg atgacgttgc aacaataatg
      541 ttttgataca agcggcctgt aaacctgaaa tgggtttaca ggccgctttt gtttgttttg
      601 tcatcaagtg agcagatgcc gatgccaaaa tggctgacat gggtcagggc agagtggctg
      661 gattgtgatt aagctgtggt gaatgacgat tttattttgg gcattggaga cgcacgatgg
      721 atgaacaaga attcaaactg tatgagctat acctgcgcat acaaacctta gaaagttcgg
      781 tggcggcgat tttgtgggcc atcagtcagc aagctaccaa cagcgatgca gtggtggcca
      841 cggttttggc acaaattcaa accagtaccg ccatgacttt ggaacacttg caggcattgg
      901 gtttcagcga agagcaattg cagcgtttac aagccacggc tttaggggtg caagacgtat
      961 acgaggcgat gaagcttaat catgagcaag tatcgtaaga ctggtgtctg tgttttaagc
     1021 ggcctgtaaa cttgaaaatg gtttacaggc cgcttggttt ttaaatcaac ttatttgatg
     1081 acaatggtgc cacgcatgct gcctgtgtgc cctgggaagg tgcagacaaa ggagtaaggc
     1141 gcggctttga tggcagccac atctaaggtc aagctgtcgg attccccccc acccaagagc
     1201 ttggttttgg caagcactcg ctcatctcct gctttgacat agtctgcggc agggcctgct
     1261 ttcatgccat ctttaatcac accgttcaaa tcactggttt tggccaccac cacgttgtgg
     1321 cccatcgctg ctttgggcat gctgcctgcg tgtttgaagt gcacggtgaa ttgttggcaa
     1381 gaagcgggta tggcgatttc tttgctgctg tattgcatgg catcgttggc gctcaaatct
     1441 gtgctgcatt cagccgttgc cgccgtgggt gctgaagctg caacaggcgc gctgtttgcg
     1501 gcctcactgg ctgtgtttgg gctgctggcg acaggcgcgg ctgcgcttgc tgcggtgttg
     1561 cttggcgcga tcgcttgttc gccgccacag gcgcttaagc ccaagcccaa tgtcaaaccc
     1621 agcgacagtt tcaaccaaaa caaatgttgc acatgagaca tggtgtttcc ttaagatgta
     1681 aattataggg taagtctact gtacccctgt gagcaaatac agccatgcgt taaatcaaat
     1741 tctgtggttt cgattgaaac aattgatttg acggtgtttt ctgtgcacgc ttacaccaga
     1801 aaaaattttg ggtaaagcgg gt
//
LOCUS       DAOMVK010000467         1820 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_18832_length_1820_cov_2.269688, whole genome shotgun sequence.
ACCESSION   DAOMVK010000467 DAOMVK010000000
VERSION     DAOMVK010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18832_length_1820_cov_2.269688"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="PK856_11695"
     CDS             <1..792
                     /locus_tag="PK856_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03072.1"
                     /translation="WGIVSLIHNLYLAAQEDEARIANEARLQKLNIPRNENRVHYNIG
                     NIDGYHINIPPGIVDGWITYNGDPNDFDFDAKEKYQRPPISYDSIIAQISFKFRVADA
                     ALLQLGTQTEQDYANDLKLKVTPWANVLSNSGEKYPEDIDKFFNNVLESYLNSVGQPL
                     TYTDKVHGLERYQFDYVNNPTAQKTWDMEEVYIYKTQQDQINTIIRCSTNPNFYAHSC
                     QQFFHMPDNEMEAYFEVRYPRELLPEWQNIEQHTIAILNGFALTH"
     gene            complement(935..>1820)
                     /locus_tag="PK856_11700"
     CDS             complement(935..>1820)
                     /locus_tag="PK856_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03073.1"
                     /translation="QAAHNHAESKPTAKPLPEGQELFTALIGESKADYYLIEFDDLAA
                     GGKSHFNWAAFFGGSYWFAARGMWGMAIMILCVATVVLILAGILASAMGPAAWAVLAA
                     WLLWRLLWLPLKANQTYFKFAQKRVKAMVRQYAKQPDRMLRLLRGTTNMGAAVGLFAG
                     VCVMALPVMGILAAIALPMYADYVDKAVWKQAHTEMVALQGPIDAVLQKNQAVQTTDP
                     KAVGYVDISGVTKLREIETDFDVVGEGYVMGRSQKNYNLQMHLVRDSVGLWHCEMHTS
                     GDNAIPRRDIPAVCKIVE"
BASE COUNT          510 a          455 c          405 g          450 t
ORIGIN      
        1 tggggcatag tgtcgctgat acacaacttg taccttgctg cgcaagaaga cgaagcaaga
       61 atcgcaaatg aagcacggct gcaaaagctg aacatccctc gaaacgaaaa tagagtccat
      121 tacaatatag ggaacattga tggttaccat atcaacattc caccaggtat tgtagatggc
      181 tggattacct acaatgggga cccaaatgat ttcgattttg atgctaaaga gaaataccaa
      241 cgcccaccca tcagttatga ttcaatcatt gcgcaaatca gttttaagtt tagggtagct
      301 gatgcggctt tattgcaatt aggtactcaa acagaacaag attatgcgaa tgatttgaaa
      361 ttaaaagtca caccttgggc aaatgttctt agcaacagtg gtgaaaaata ccctgaagat
      421 attgataaat tttttaacaa tgttttggaa agctacctca attctgtcgg acagccatta
      481 acatacactg ataaagttca cggcctagaa cgctatcaat ttgattatgt taataatcct
      541 acagcacaaa aaacatggga tatggaggag gtctacattt ataaaactca gcaagaccaa
      601 atcaacacca ttatccgttg ttcaacaaac ccgaattttt atgctcattc ctgccagcaa
      661 tttttccaca tgcctgacaa tgaaatggaa gcttattttg aagtccgcta tcctcgcgaa
      721 cttttaccag aatggcaaaa tattgagcag cacaccatag ccatactcaa cggatttgcc
      781 ttaacccact gatgccctcg tgcaaatcaa gctcaatggt ctgacattct ctcaataaga
      841 aacaagaggc atttttaaat agccgctaga agaatcatgt gattaaaagc ggcctgtaag
      901 ggtttacagg ccgcttttaa tctttgctgc gcattcattc aacaattttg catacagcag
      961 gaatatcgcg tctggggatg gcgttgtcgc cggaggtgtg catttcgcaa tgccacaagc
     1021 ctacgctgtc gcgcaccaaa tgcatttgaa gattgtaatt tttctgactg cgccccatca
     1081 catagccctc acccaccacg tcaaaatcgg tttcgatttc tctgagcttg gtcacgccgc
     1141 tgatgtccac atagcctacg gctttggggt cggtggtttg cactgcttgg tttttttgta
     1201 atacggcgtc tatcgggcct tgcaaggcca ccatctcagt gtgcgcttgc ttccaaacgg
     1261 ctttgtccac gtagtcggca tacatgggca aggcgatggc agcgagtatg cccatcacag
     1321 gaagcgccat cacgcacaca cccgcaaaca aaccgacggc cgcgcccata ttggtggtac
     1381 cgcgcagtaa gcgcagcatg cggtcgggtt gtttcgcgta ttggcgcacc atcgctttca
     1441 cccgtttttg ggcgaattta aaataggttt ggttggcttt gagcggcaac cacagcaacc
     1501 gccacaacaa ccacgcagcc aataccgccc aagcagcagg ccccatggcg ctggccaaga
     1561 tacctgccaa aatcaacacc accgtggcca cacacaaaat catgatggcc atgccccaca
     1621 tgccacgagc ggcaaaccaa tagctgccac caaaaaatgc ggcccagtta aagtggcttt
     1681 ttccgcctgc ggccaagtca tcaaattcga tcaggtagta atcggccttg ctctcgccga
     1741 tgagggcggt aaacagctcc tgcccttcgg gcaaaggctt ggctgtgggt ttggattcgg
     1801 cgtgattgtg ggcagcttgg
//
LOCUS       DAOMVK010000468         1814 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_18929_length_1814_cov_2.620239, whole genome shotgun sequence.
ACCESSION   DAOMVK010000468 DAOMVK010000000
VERSION     DAOMVK010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18929_length_1814_cov_2.620239"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..808
                     /gene="ung"
                     /locus_tag="PK856_11705"
     CDS             137..808
                     /gene="ung"
                     /locus_tag="PK856_11705"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRL03074.1"
                     /translation="MQTNPANWQDTLSEEKQQPYFQDLIHKVNQERADGKVIYPPNAE
                     VFQAFVSTPLADIKVVILGQDPYHGPEQAHGLAFSVRQGVKIPPSLRNMYKELSTDIQ
                     GFKIPTHGNLHAWAEQGVLLLNTVLTVEAGQAHSHAKWGWERFTDKVVDVINQHCQNV
                     VFLLWGSHAQKKGAMIDRTRHLVLETVHPSPLSAHRGFLGCQHFSQTNQYLQHTKQTP
                     IHWQI"
     gene            858..>1814
                     /locus_tag="PK856_11710"
     CDS             858..>1814
                     /locus_tag="PK856_11710"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HRL03075.1"
                     /translation="MSTASSTPRQNHRWLGKPSLSSAIRFGVWIASIVYVVLYLVGSG
                     LLDFFLAEGTQNVVFVIALLAALIGAYDVKQPKHSVLRNYPVIGHFRWLFEGIRPEIR
                     QYLIEADDEALPFTRSQRSLVYQRAKAVSADKPFGTIVDVYQNGYEFMVHSILPAPQR
                     NPEDFRVQIGNEQCAQPYSASIFNISAMSFGSLSANAILALNHGAARGRFYHDTGEGS
                     ISPYHLQGGGDLVWELGSGYFGCRTNDGRFDPEKFAKQSVLPNVKMIEIKMSQGAKPG
                     HGGILPKEKITEQIAQVRGIPRDRDCVSPASHSAFTTPLEMMA"
BASE COUNT          463 a          398 c          462 g          491 t
ORIGIN      
        1 cgtgtggtga atttcatgtg caggcttcct gtgcaagcaa gtcattctgt gtgcatacga
       61 tgctgcctga gcctcacaag ttcaggccgc ttactgtaaa atttaaccct ctttcaatga
      121 aacagataaa caacagatgc aaacaaaccc agccaattgg caagacacct tgtctgaaga
      181 gaagcaacag ccttattttc aggatttaat tcacaaagtc aaccaagaac gtgctgatgg
      241 caaagtgatt tatccaccga atgctgaggt gtttcaagca tttgtgagca cgcctttggc
      301 agacattaag gtggtgattt tggggcaaga cccttatcat gggccagaac aagcccatgg
      361 tttggcattt tcggtgcgac aaggggtgaa gataccgcca tcgctgcgca atatgtacaa
      421 ggaattgagc accgatattc aaggctttaa aatccccaca catggcaatt tgcatgcttg
      481 ggccgaacaa ggagtgttgc ttttaaacac ggttttgacc gtagaagcag ggcaggcgca
      541 ctcacatgcc aaatggggtt gggagcggtt tacggataag gtggtcgatg tcattaacca
      601 acactgccag aatgtggtgt ttttgttgtg gggcagccat gctcagaaaa aaggagccat
      661 gatagacagg acgcgtcatt tggttttaga gacggtgcac ccatcgccat tgtcggctca
      721 ccgaggtttt ttgggctgcc aacatttttc acaaaccaat caatacttac aacacaccaa
      781 acaaacgccg attcattggc aaatataaac catgcggcct gtaaggttta caggccgctt
      841 ataaaatgga aaataccatg agtacagctt cttcaacccc aagacaaaat caccgttggt
      901 tgggcaaacc ttcactttcc agtgccatac gctttggtgt gtggattgcg tccattgttt
      961 atgtggtttt gtatttggta ggcagtggtt tgttggactt ttttttagcc gaaggcacgc
     1021 agaatgtggt gtttgtcatt gcattgttgg ccgcattgat aggcgcttac gatgtgaagc
     1081 agcctaagca cagtgtgttg cgaaattatc cggttattgg gcattttcgt tggttgtttg
     1141 aagggatacg gcctgaaatt cgtcagtatt tgatagaggc cgatgatgaa gctttgccgt
     1201 ttacccgttc gcagcgctca ttggtgtatc agcgtgccaa agccgtgagt gccgataagc
     1261 ctttcggtac cattgttgat gtctaccaaa atggctatga attcatggtg cactccattt
     1321 tacctgctcc acaacgcaac cctgaggatt tccgtgtgca aattggcaat gaacagtgtg
     1381 cacagcctta ttctgcttcg atattcaata tttcggcgat gagttttggc agcttgagcg
     1441 ccaatgcgat tttggccttg aatcatggcg cggcgcgcgg ccgcttttac cacgacacgg
     1501 gtgagggcag catcagccct taccatttac aaggtggtgg cgatttggtg tgggagctgg
     1561 gcagtggtta tttcggttgt cgcaccaatg atggcaggtt tgaccctgag aaatttgcca
     1621 aacaatcggt gttgccgaat gtgaaaatga ttgaaatcaa aatgagccaa ggcgcgaagc
     1681 ctggacatgg tggtattttg cctaaagaaa aaatcaccga acaaattgcc caagtgcgtg
     1741 gcattccacg tgatcgcgat tgcgtgtcac ccgcttcaca ttccgccttt actacgcctt
     1801 tggaaatgat ggca
//
LOCUS       DAOMVK010000469         1810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_18978_length_1810_cov_2.603419, whole genome shotgun sequence.
ACCESSION   DAOMVK010000469 DAOMVK010000000
VERSION     DAOMVK010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18978_length_1810_cov_2.603419"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..161
                     /locus_tag="PK856_11715"
     CDS             6..161
                     /locus_tag="PK856_11715"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRL03076.1"
                     /translation="MQGQFELWVGERHYLLAQGDSFSFSSTEPHRYGNPGAEPCKIVW
                     VITPPTY"
     gene            509..859
                     /locus_tag="PK856_11720"
     CDS             509..859
                     /locus_tag="PK856_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRL03077.1"
                     /translation="MDIHHINVQEADFSLFADVGAPVGTPVPQARTAAEQSLPWAEVG
                     IWECTAGKMERLIAEAEQMYFISGKARFTPVGGETIEIRAGDSLFLPAHTKGVWEIDE
                     TVRKSYAVFHNSAD"
     gene            904..>1810
                     /locus_tag="PK856_11725"
     CDS             904..>1810
                     /locus_tag="PK856_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HRL03078.1"
                     /translation="MAQAALEHLFSPIKIRNLTVKNRIMSTGHDTTMPTEGIINERLL
                     AYQRARAEGGAGLIVLQVSGVHESARYTTHLLMATEPNCVDGYRKMADMCHAHGTTIF
                     AQIFHPGREIMEAGEGLLAVAYSASAVPNERFHVMPKPLNQRMIDEISAGYVQAARYM
                     HEAGIDGVEFVASHGYLPAQFMSTQVNLREDQYGGSLENRLRFSVDILKAIRAATSDD
                     FVIGMRISADELDGGGLDADEVLAICQALEPHLDYVNLTLGTSASLGGAVHIAPPMAF
                     KAGYIAEQTKVFKDTLNIPVLIAGRI"
BASE COUNT          465 a          468 c          461 g          416 t
ORIGIN      
        1 atgtgatgca aggccagttt gaattgtggg tgggcgagcg ccattatttg ctcgcgcaag
       61 gcgattcttt cagcttcagc agcaccgagc cacaccgtta cggcaatcct ggtgctgagc
      121 cttgcaaaat cgtgtgggtg attacgccgc ccacgtattg agccattttt ttgctgccca
      181 acacaacact gccacctcac ttgaggtggt attttattgt gcagattcaa agcactgccc
      241 aattcaaatc aatgcaccag ccatgcgcaa atctgcccgc ggcatttagt ggttctcact
      301 gcttaaattc acataatgcc attgctgcag cgatgtaaag acaaagctta caacactcgt
      361 gcaaggtgcg gcctcaattt tattaaaagt aaaaaaatat attgattttt tactttttaa
      421 gccctagtct atcctcagtc tacacaagca gcctgctaac ttggctgcga caacacatta
      481 aaaaaccgat aaaaaagagg agttacacat ggacattcac cacataaacg tacaagaagc
      541 cgatttcagt ctatttgccg atgtgggcgc gcctgtgggc acacccgtac cacaagcgcg
      601 caccgcagca gagcaaagct tgccgtgggc tgaagtgggc atttgggaat gcactgcagg
      661 caaaatggaa cgactcattg ccgaagccga acaaatgtat ttcatcagtg gcaaagcccg
      721 ctttacgcca gtagggggag aaaccatcga aattcgtgcg ggtgacagct tgtttttgcc
      781 tgcccacacc aaaggcgtgt gggaaatcga cgaaaccgtg cgcaagtctt atgcggtgtt
      841 tcacaacagc gccgactaat acctcgccac aataataaaa ccaacgtcgc ttaggagaga
      901 gacatggcac aagctgcatt ggaacacttg ttcagcccaa tcaaaatccg caacctcacg
      961 gtgaaaaacc gcatcatgtc cacgggccat gacaccacca tgccgaccga aggcatcatc
     1021 aatgagcgtt tattggccta ccaacgcgcc cgtgccgaag gcggcgcagg cttgattgtg
     1081 ttgcaagtgt cgggtgtgca tgaaagcgcc cgctatacca cgcatttgtt gatggccacc
     1141 gagccgaact gtgtggatgg ctaccgcaaa atggccgaca tgtgccatgc acacggcacc
     1201 accatctttg cacaaatttt tcacccaggt cgcgaaatca tggaagcggg cgagggcttg
     1261 ctggccgtgg cttattcggc ttcggctgtg cccaatgagc gctttcacgt gatgcccaaa
     1321 cctttgaatc agcgcatgat agatgagatt tccgcaggtt acgtacaggc cgctcgttac
     1381 atgcatgagg caggcataga cggggtggaa tttgttgcca gtcatggcta tttgcccgcg
     1441 caattcatga gcacgcaagt caatttgcgc gaagaccaat acggtggcag cttggaaaac
     1501 cgcttgcgtt tcagcgtgga cattttgaaa gcgatacgcg ccgccacttc ggatgatttt
     1561 gtcatcggca tgcgcatttc cgctgacgaa ctcgacggcg gcggtttgga tgcagacgag
     1621 gtattggcca tttgtcaggc tttggagccg catttggact atgtgaacct gactttgggc
     1681 acatccgcaa gcttgggtgg cgcggtacac attgcgccgc cgatggcatt caaagcgggc
     1741 tatattgccg agcaaaccaa agtattcaaa gacacattga acatacctgt gttgattgca
     1801 gggcgcatca
//
LOCUS       DAOMVK010000470         1807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_19030_length_1807_cov_1.804795, whole genome shotgun sequence.
ACCESSION   DAOMVK010000470 DAOMVK010000000
VERSION     DAOMVK010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19030_length_1807_cov_1.804795"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PK856_11730"
     CDS             <1..277
                     /locus_tag="PK856_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003769197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HRL03079.1"
                     /translation="YDSKNMMHTFRLLHTALDIAKHGELRVWRDNRDDLLAIKAGEFG
                     YDDLLQRAQALMLEVEQAFARSHLPEQPDLMQLARVLVEVRQAFYQQ"
     gene            complement(359..883)
                     /locus_tag="PK856_11735"
     CDS             complement(359..883)
                     /locus_tag="PK856_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HRL03080.1"
                     /translation="MFEHKIIFTGPVGAGKTTLIKAFSGTEPVLTEVRASDEVSQLKE
                     NTTVAMDYGSVILDDETKVHLYGTPGQKRFDFMWDILSRGSMGLAILINAKAPKPLDD
                     LRFYLDAFKGLIVDQDLPVVVGINQVNKDQPEPSRAEFKAVAEEFRLGIPILMIDARE
                     EKDVRRMVMTLLFS"
     gene            complement(876..>1807)
                     /locus_tag="PK856_11740"
     CDS             complement(876..>1807)
                     /locus_tag="PK856_11740"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRL03081.1"
                     /translation="SQKLKIEPLTQHIHQLRHSANQLNLQMLVYLSDEFEQHISDLKQ
                     QNYLNRDDFLPLDITLDQMDHVLQTITQVHARLQQTEVAATAAPTALSIASPETDMQR
                     WQHDAQQMAKRHHKSIHLICTHASFQNLPLHLVEKVQDLTWNLISNAVVHGIESPEVR
                     ESMAKTEHGQVTVSLLDMGTEYRLSVEDDGKGIDYEAIQAQAVDLGWCQNNADAPINK
                     TQLLHYLFQSDFSTLAQMNSDAGDGMGLYWVQTWVDEMGGHLDVVTQAHQFTRFIITL
                     PKASHSPAALVSDEEEARFKQAADANDEEHYIV"
BASE COUNT          394 a          409 c          497 g          507 t
ORIGIN      
        1 atacgacagc aaaaacatga tgcacacgtt tcgcttattg cacacggctt tggacatcgc
       61 caaacacggc gagctgcggg tgtggcgcga caaccgcgat gatttgttgg cgattaaagc
      121 cggtgaattt ggctatgatg atttgctgca acgagcgcag gctttgatgc tggaggtgga
      181 gcaagccttt gcccgcagcc acttgcctga gcagcctgat ttgatgcagt tggctagggt
      241 attggtcgag gtgcggcagg cattttatca gcagtgatcg ggcataaaaa ggcggcctgt
      301 aaactgtgtg atggtttaca ggccgctagc ccttgtgtgt gtagcggggc gtgtgaaatt
      361 aactgaataa caaggtcatc accatgcggc gcacgtcttt ttcttcacgc gcgtctatca
      421 tcaaaatggg gatgcccaag cgaaactctt cggccacggc tttgaattcg gcgcggctcg
      481 gttcgggttg gtctttgttg acttggttga tgcccaccac cacgggcaag tcttggtcta
      541 caatcaagcc tttgaacgca tccaaataaa agcgcaaatc gtctaagggt ttgggggctt
      601 tggcattgat taaaatcgcc aagcccatgc tgccgcggct caaaatgtcc cacatgaagt
      661 caaagcgttt ttgcccaggt gtgccataca aatgcacttt ggtttcatca tctaaaatca
      721 ctgagccata gtccatggcg acggtggtgt tttcttttaa ctgtgacact tcatcagaag
      781 ctcgtacttc ggttaacaca ggctcggtgc cgctgaatgc tttgattaac gtggttttgc
      841 cagcgcctac tgggccggta aaaatgattt tgtgttcaaa caatgtaatg ctcctcatcg
      901 ttggcatcag cggcttgttt aaaacgggct tcttcctcat cgctcaccaa agcagcgggt
      961 gagtggctgg ctttgggcag ggtgatgatg aagcgggtaa attggtgggc ttgtgtgacc
     1021 acatcgaggt ggccgcccat ttcgtctacc caagtttgca cccaatacaa gcccatgcca
     1081 tcgccagcat cgctgttcat ttgggccaag gttgaaaaat cggattgaaa caaatagtgc
     1141 aacaattgtg ttttgttgat gggggcgtcg gcattgtttt ggcaccagcc caagtcaacg
     1201 gcttgtgcct gtatggcttc gtagtctatg cctttgccat cgtcttccac gctcaagcgg
     1261 tattctgtgc ccatgtccag caaagacact gtcacttggc cgtgttcggt tttggccatg
     1321 gattcacgca cctctgggct ttctatgccg tgtaccaccg cattgctgat caagttccag
     1381 gttaaatctt ggactttctc caccaaatgc aggggcaggt tttgaaagga agcatgggtg
     1441 caaatcaagt gtatggattt gtggtggcgt ttggccattt gttgggcatc gtgttgccag
     1501 cgctgcatat cggtttcagg cgaagcgatg ctgagggcag taggcgcagc ggtggctgcc
     1561 acttcggttt gttgtaatcg ggcatgcact tgggtgatgg tttgcaacac atggtccatc
     1621 tgatccaagg tgatgtccag cggcagaaaa tcatcgcgat tgaggtagtt ttgttgtttt
     1681 aaatcgctga tgtgttgctc aaactcgtcg cttaaataca ccaacatttg taaattcaat
     1741 tgattggcac tgtgtctgag ttggtgtatg tgttgggtta aaggctcaat tttgagtttt
     1801 tgcgact
//
LOCUS       DAOMVK010000471         1806 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_19039_length_1806_cov_3.227870, whole genome shotgun sequence.
ACCESSION   DAOMVK010000471 DAOMVK010000000
VERSION     DAOMVK010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19039_length_1806_cov_3.227870"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="PK856_11745"
     CDS             complement(<1..913)
                     /locus_tag="PK856_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HRL03082.1"
                     /translation="MNSAKLPATTLAALGIVYGDIGTSVLYTLKECFAAAHIELNAMN
                     VMGFVSLIFWSIILVVTIKYVLIVLRADNHGEGGIMVLMQLAKQYLHGKPIAFVLFMG
                     LLGAALFYGDAMITPAISLLSASEGLTVINPNWHAAVLPLALTILVVLFAIQRFGSQK
                     IGVVFGPIMLLWFATLAVLGIRGILLHPAILHSLNPYYALVFISHHGFAGWIGLGAVV
                     LALTGAEALYADMGHFGRKPIQIAWFGLVLPSLVLNYMGQGGLLLAKPDSLVNPFFML
                     APEWGRWPLLLLAAFATIIASQAVISGA"
     gene            complement(991..1485)
                     /locus_tag="PK856_11750"
     CDS             complement(991..1485)
                     /locus_tag="PK856_11750"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRL03083.1"
                     /translation="MNVYQYQTDSPTGRLRLFADSTQLLAILWENDAPKRVVLPAAKT
                     VQEHEVLQEAAKQLQAYFAGTHTAFDVPLRLQGTQFQQQVWQALQTIPYGQTRTYKDI
                     AAAIGRPSASRAVGAAIGRNPVSIIVPCHRVLGVTGNLTGFAGGLDNKRLLLDLEQDH
                     CPLV"
BASE COUNT          493 a          440 c          456 g          417 t
ORIGIN      
        1 atgcccctga aatcacggcc tgactggcga tgatggtggc aaatgcggcc agtaaaagca
       61 aaggccagcg gccccattca ggcgcaagca tgaaaaatgg attgaccaag ctgtcgggtt
      121 tggcaagcag caagccgcct tgacccatgt agttcaacac caagctgggc aacaccaagc
      181 caaaccaagc gatttgtatc ggtttgcgcc caaaatggcc catgtcggca tacagtgctt
      241 ccgcgcccgt gagcgccaaa accaccgcac ccaagcctat ccagcctgca aagccatggt
      301 ggctaatgaa gaccaaggcg taataagggt tgaggctgtg caaaatcgca gggtggagca
      361 agatgccgcg tatgcccaat accgccaaag tggcaaacca caacaacatg atggggccaa
      421 acaccacgcc gattttttgg ctgccaaagc gttgaatggc aaacaaaacc accaaaatgg
      481 tcagcgccaa aggcaaaaca gcggcatgcc aattgggatt gatgacggtc aaaccttcgg
      541 aagccgacag caaagaaatc gcgggcgtaa tcatcgcatc gccataaaac aaggcagcac
      601 cgagcaagcc catgaacagc acaaacgcaa tcggtttgcc atgcaaatac tgtttggcca
      661 attgcatcaa caccatgatg ccgccttcgc cgtggttgtc ggcgcgcagc acaatgagca
      721 cgtatttgat ggtcaccacc aaaataatcg accaaaaaat cagcgacaca aagcccatca
      781 cattcatggc gttgagttcg atgtgggccg cagcgaaaca ctcttttaag gtgtaaagca
      841 cgctggtgcc aatgtcgcca tacacaatgc caagagcggc taaggtggtg gcagggagtt
      901 ttgcggaatt catgatgaag aagctcacct ttaagtggca gccaagctgc gagaacaatc
      961 aaatcgggta gggatgggca tgcgcgtggc tcagaccaat gggcagtggt cttgttccaa
     1021 atcaagcagc aaacgcttgt tgtctaagcc gcctgcaaag ccagttaaat tgccagtcac
     1081 cccaagtacg cggtggcacg gcacaataat cgacacagga tttctgccaa tggctgcacc
     1141 cacagcgcga ctggcgctgg ggcggccgat ggcggcggcg atgtctttgt aggtgcgggt
     1201 ctgaccatag ggaatggttt gcagtgcttg ccaaacctgt tgctgaaatt gagtgccttg
     1261 caaacgcaaa ggcacatcaa atgcggtatg agtgcctgca aaatacgctt gcaattgctt
     1321 tgcggcctct tgcaatactt catgttcttg cacggttttt gccgcaggca ataccacccg
     1381 tttgggtgca tcgttttccc acaagatggc caatagctgc gttgagtctg caaagagtct
     1441 taagcggcct gtagggctat ctgtttgata ttgatacaca ttcatggtta ctcccaagct
     1501 tgggcgtcct gtaaagctcg aatcgtattc cttttttatc caaatcgcac catttttgtt
     1561 gcgatgcagc ataaataaat gttttttatc atgatttttc cgtgtgtatt ttgccctagg
     1621 ctgaaaattt gcagattttg ttattttaag cttttgattt atttaaattt tgttgatttg
     1681 gtgtcagtat ctcaggtggg ctgtattttg ctgacaaaat ggcatgtgat gcgcatcagc
     1741 acagatttaa caaaaatggc gataagcatt gataagggct atgacaatca attcatctga
     1801 acttag
//
LOCUS       DAOMVK010000472         1806 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_19040_length_1806_cov_2.938321, whole genome shotgun sequence.
ACCESSION   DAOMVK010000472 DAOMVK010000000
VERSION     DAOMVK010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19040_length_1806_cov_2.938321"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..144
                     /locus_tag="PK856_11755"
     CDS             4..144
                     /locus_tag="PK856_11755"
                     /inference="COORDINATES: protein motif:HMM:NF013304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HRL03084.1"
                     /translation="MNFWASLFVLSRLLYIVLYIKDMPSFRSLSWCAGLVCILALFIA
                     AI"
     gene            225..1253
                     /gene="gap"
                     /locus_tag="PK856_11760"
     CDS             225..1253
                     /gene="gap"
                     /locus_tag="PK856_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRL03085.1"
                     /translation="MTIKVAINGYGRIGRQVLRAIYDYNLRNVFEVVGVNASGDLATN
                     AHLTKFDTVHGRFGAEVSHQDNALVINGDVIPFFSTRNPAELPWKELAVDLVLECTGA
                     FTTKEKCQAHIDAGAKKVLISAPGGADVDATVVYGVNHDVLTPEMTVVSNASCTTNCL
                     APIAKALNDALVIEKGLMTTIHAFTNDQVLTDVRHKDLRRARSATENMIPTKTGAAKA
                     VGLVLPTLNGKLDGFSVRVPTINVSLVDLTFEAGRDTSVEEVNEIVKKAAEGSMKHVL
                     GYNTLPLVSTDFNHTTEGSTFDSTLTKVSNGNMVKVLAWYDNEWGFSCQMLRTAAVMF
                     GESVVELK"
     gene            1500..>1806
                     /locus_tag="PK856_11765"
     CDS             1500..>1806
                     /locus_tag="PK856_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HRL03086.1"
                     /translation="MAYDNDNIFAKILRGEIPAFKVHEDEHVLAFMDIMPQADGHVLV
                     IPKAPAVTLLDLPADAAAITIQAVQKVARGMKTALAAEGIVLMQLSGEAAGQTVPHVH
                     "
BASE COUNT          439 a          419 c          480 g          468 t
ORIGIN      
        1 gccatcaatt tttgggcgag tttgtttgtg ttaagccgct tgctgtacat tgttttgtac
       61 atcaaagaca tgcccagctt ccgctcattg tcttggtgtg caggattggt gtgcatcttg
      121 gccttgttta ttgcggcaat ttaggtaaat agcccgcaaa acaccgacga ttttggtata
      181 atcgccggcg ttttattata caaatattat taaggacgct tctaatgacg attaaagtcg
      241 ctatcaacgg ttatggccgc atcggccgtc aagttttacg tgccatttac gattacaatt
      301 tgcgcaatgt gtttgaagtg gttggcgtga atgcatctgg cgacttggca accaatgctc
      361 acttgaccaa atttgatacc gtacatggtc gctttggtgc agaagtgtct caccaagaca
      421 atgctttggt gattaacggt gacgtgattc ctttcttctc tacccgcaat cctgctgaat
      481 tgccttggaa agaattggct gtggatttgg tgttggagtg cacaggcgcg ttcaccacca
      541 aagaaaaatg ccaagcccac attgatgcgg gtgcgaaaaa agtcttgatt tcagctcctg
      601 gcggcgcgga tgtggacgcg actgttgtgt acggcgtgaa ccacgatgtg ttgaccccag
      661 aaatgaccgt ggtgtctaac gcgtcttgca ccaccaactg cttggcacct attgccaaag
      721 ccttgaacga tgcgttggtg atagaaaaag gcttgatgac caccatccat gccttcacca
      781 acgaccaagt gttgaccgat gtgcgccaca aagatttgcg tcgcgcccgt tctgccactg
      841 aaaacatgat tccgaccaaa acaggtgcag ccaaagccgt gggcttggta ttgcctactt
      901 tgaatggcaa attggacggt ttctcagtgc gcgtgcctac cattaacgtg tctttggtgg
      961 atttgacgtt tgaagcaggt cgcgacactt cggttgaaga agtcaacgaa atcgtgaaaa
     1021 aagccgcaga aggcagcatg aagcacgtgt tgggctacaa caccttgcct ttggtttcga
     1081 ccgatttcaa ccacaccacc gaaggttcta ctttcgattc aaccttaacc aaagtgtcta
     1141 acggcaatat ggtgaaagta ttggcttggt acgacaacga atggggcttc agctgccaaa
     1201 tgttgcgcac agccgctgtc atgttcggcg agagcgtggt ggaattgaaa taagccaagg
     1261 cttatttgaa acaccgaaaa cagcctgcag cgatgcgggc tgtttttgcg tgcacagcgt
     1321 gtgcaatcag atgtcgaggc tgtgcttgtg cgcatccaga ttgggctgga taagcggcct
     1381 gtaaaggctg agaaaacgct ttacaggcct tgtggcatta cagaccatgc atgaggcttg
     1441 ctctgtggcg gttggcttgc taaggtaagc catttgcagc gtttacttaa aggaagaaca
     1501 tggcttacga caacgacaat atttttgcca agattttgcg cggggaaatc cccgctttta
     1561 aagtgcatga agacgagcat gtgctggcgt ttatggacat catgccgcag gccgatggcc
     1621 atgtgttggt gatacccaaa gccccagcgg tgacgctgtt ggatttgcct gccgatgctg
     1681 ccgccatcac catacaagcc gtgcaaaaag tggcgcgtgg catgaaaaca gccttggccg
     1741 ccgaaggcat cgtattgatg caattgagtg gcgaagccgc aggccaaacc gtgccgcatg
     1801 tgcatt
//
LOCUS       DAOMVK010000473         1805 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_19055_length_1805_cov_2.536571, whole genome shotgun sequence.
ACCESSION   DAOMVK010000473 DAOMVK010000000
VERSION     DAOMVK010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19055_length_1805_cov_2.536571"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1805
                     /gene="purL"
                     /locus_tag="PK856_11770"
                     /gene_synonym="purI"
     CDS             <1..>1805
                     /gene="purL"
                     /locus_tag="PK856_11770"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HRL03087.1"
                     /translation="RVLRLPTVASKNFLISIGDRSVGGMTYQDQLVGRFQTPVANAAI
                     TMMGFNTYQGEAMSMGEKAPLALFDAAASGRMAVAEALTNIAATAIGDIGNIKLSANW
                     MAPCGVAGEDEKLYRTVEAVSEICQTLGVSIPVGKDSLSMKTAWVDHGEQKTVVAPLS
                     CVITAFAPVLDVRKTVTPELKTQDSSLLLVDLGCGQNRLGGSALTQVHKTLQGVSPDV
                     NDQIALGNFFKVVQQLVAQGDVLAYHDRGDGGLLTTLAEMMFASHLGLTINVAKILEK
                     SHSQVHNLNDTIVRCLFSEELGAVLQVAADKVAAVQAAFAAVGLGDCVYNIGQTNASD
                     RLLVQNGNMILLNEARTDMQKAWTETSYQIQRLRDNPACADSEFALIGDDKRTALFAH
                     TTFDVNADITAPYINSGAKPKIAILREQGVNGQLEMAAAFTKAGFDAYDVHMSDLFAG
                     RHHLQDFQALAACGGFSYGDVLGAGQGWAKSILFNPALRDMFEAYFKRADTLSLGVCN
                     GCQMMSQLADIIPGANNWPRFARNESEQYEARLSMVTIPESPSVFLSALAGSSVPVVV
                     SHGEGRADFAQHGNIDAVNIALQYVDGQNHVTQTY"
BASE COUNT          427 a          483 c          472 g          423 t
ORIGIN      
        1 accgcgtgtt gcgcttgcct actgtggcca gcaaaaactt cttgatttcc attggtgacc
       61 gctcagtcgg cggcatgact taccaagacc aattggtagg ccgcttccaa accccagtgg
      121 ccaatgccgc catcaccatg atgggcttta acacctacca aggtgaagcc atgtccatgg
      181 gcgaaaaagc tccattagct ttgtttgatg ccgccgcttc aggccgcatg gcagtggccg
      241 aagccttaac caatattgcg gctacggcca ttggtgacat cggcaacatc aagttgtctg
      301 ccaactggat ggcaccttgc ggcgtggccg gtgaagacga aaaactctac cgcactgttg
      361 aagccgtgtc tgaaatttgc caaaccttgg gcgtgagcat tcctgtgggc aaagactctt
      421 tgtcgatgaa aactgcttgg gttgaccacg gcgaacaaaa aaccgtggtg gcaccactgt
      481 cttgtgtgat taccgcgttt gcacccgtat tagacgtgcg caaaaccgtt accccagaat
      541 tgaaaaccca agacagcagc ttgttgttgg tggatttggg ttgtggccaa aaccgcttgg
      601 gcggcagtgc tttgacccaa gtgcacaaga ctttgcaagg cgtttcccct gatgtgaatg
      661 accaaatcgc tttgggcaac ttcttcaaag tggtgcaaca attggttgcc caaggggatg
      721 tgttggccta ccacgaccgt ggcgatggcg gcttgctcac cactttggcg gaaatgatgt
      781 ttgcctcaca cctgggcttg accatcaacg tggccaagat actggaaaaa tcgcacagcc
      841 aagtccacaa cctcaacgac accatcgtac gctgcttgtt cagtgaggaa ttgggcgcgg
      901 tattgcaagt ggctgctgac aaagtggctg ccgtacaggc cgcttttgcc gcagttggct
      961 tgggcgactg cgtgtacaac attggtcaaa ccaacgcttc tgaccgcttg ttggtgcaaa
     1021 acggcaacat gattttgctc aacgaagccc gcacagacat gcaaaaagcg tggacagaaa
     1081 cttcatacca aatccaacgc ttgcgtgaca atccagcttg tgccgacagc gaatttgcct
     1141 tgataggcga tgacaaacgc accgccttgt ttgcccacac cacgtttgat gtgaacgccg
     1201 acatcaccgc gccttacatc aattcgggtg cgaaacccaa aatcgccatc ttgcgcgagc
     1261 aaggcgtgaa tggccaattg gaaatggcgg cagcgtttac caaagcaggc ttcgatgctt
     1321 acgacgtgca catgagcgat ttgtttgcgg gtcgtcacca cttgcaagac ttccaagcct
     1381 tagcggcttg tggcggcttc tcgtatggcg acgtcttggg tgcaggccaa ggctgggcca
     1441 aatccatctt gttcaatcct gcattgcgcg acatgtttga agcctacttc aaacgtgcgg
     1501 acacactgtc attgggcgtg tgtaacggtt gccaaatgat gagccaattg gccgacatca
     1561 ttccaggcgc caataactgg ccacgttttg cccgcaacga atctgaacaa tatgaagcac
     1621 gtttgagcat ggtcaccatt cctgaatcgc catcggtatt tttgtcagcc ttggctggta
     1681 gctctgtgcc tgtggtggtg agccacggtg aaggtcgcgc cgattttgcc caacatggca
     1741 acattgatgc ggtcaacatt gccttgcaat atgttgatgg ccaaaaccac gtgacccaaa
     1801 cctac
//
LOCUS       DAOMVK010000474         1802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_19094_length_1802_cov_2.371494, whole genome shotgun sequence.
ACCESSION   DAOMVK010000474 DAOMVK010000000
VERSION     DAOMVK010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19094_length_1802_cov_2.371494"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /locus_tag="PK856_11775"
     CDS             <1..1092
                     /locus_tag="PK856_11775"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRL03088.1"
                     /translation="GTNMDAQSQALRSGREIVVATVGRLLDHVGQKNIQLNKVDILVL
                     DEADRMLDMGFIDDIRKIITMLPKNRQILLFSATFAPEIKRLANDFMHQPETIEVAAQ
                     NTTNKNVEQHVIAVDGNRKRHLLERLIVDLDMSQVIVFCKTKISADQVTRDLVRKNIS
                     AKAIHGDKAQQNRTETLAAFKEGTVRVLVATDVAARGLDISELPFVINYEMPTNAEDY
                     VHRIGRTGRAGAEGVALSLMEESEQKMFEAICKLTKQKLPLDKIEGFEPRWMRENTGQ
                     ASAVKLANDNKAADNKAKVAPTASNESSKDKSRANVVRKLNTDIKTVAKAADERPDAP
                     LIKGRRRRGVRHQRQTCALLQANFGVNPR"
     gene            1102..>1802
                     /locus_tag="PK856_11780"
     CDS             1102..>1802
                     /locus_tag="PK856_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRL03089.1"
                     /translation="MSENETPTLKAFSLLECLAASDHAMSLTELARIMDLPKPSIHRH
                     LAALEAGGLIIRDAGTKHAYTIGPRLTRLSSTVLNHGDVQRQRHAILKGLVADIGQTC
                     NLTMLVKSQVLYLDRMESPSPLRLDLQPGTTVPAHSCASGKLFLSKMPKDKRHALLEQ
                     TKLEALTPNTLTNIEELDAELERINVRNVSIDNEEFVLGITCIAVPIFDDKQNCIAAI
                     AMHGQVATTPLTVSLS"
BASE COUNT          492 a          448 c          463 g          399 t
ORIGIN      
        1 ggcaccaata tggatgccca atcgcaagcc ttgcgcagcg gtcgtgaaat cgtggtagca
       61 accgtgggtc gtttgctcga ccatgtgggt caaaaaaaca tccagttgaa caaagtggac
      121 atcttggtgt tggatgaagc cgaccgcatg ctagacatgg gttttattga cgacatccgc
      181 aaaatcatca ccatgttgcc caaaaatcgc caaatcttgc tgttttcagc cacattcgca
      241 cctgaaatca agcgtttggc caatgatttc atgcaccagc ctgaaaccat cgaagtggcg
      301 gcacagaaca ccaccaataa aaatgttgaa caacatgtta ttgcagtgga tggcaaccgc
      361 aaacgccatt tgttagagcg cttgattgta gatttggaca tgtcgcaagt catcgtgttt
      421 tgtaaaacca aaatcagtgc cgaccaagtg acgcgtgatt tggtgcgtaa aaacatcagt
      481 gccaaagcca ttcatggtga caaggcgcaa caaaaccgca ccgaaacctt ggcagcgttt
      541 aaagaaggca cggtgcgtgt cttggtggcc accgacgtgg ctgcgcgcgg tttggacatc
      601 agcgaattgc ctttcgtcat caattatgaa atgccgacca atgctgagga ctatgtgcac
      661 cgcatcggtc gtacaggcag agcaggggct gagggtgtgg ctttgtcatt gatggaagag
      721 tctgaacaaa aaatgtttga ggccatttgc aagttgacca agcaaaaact gcctttggat
      781 aaaattgagg gttttgagcc gcgttggatg cgtgagaaca cgggccaagc ttcggcggtt
      841 aaattggcga atgacaacaa agccgctgac aacaaagcca aagtcgcgcc aacagccagc
      901 aatgagagca gcaaagacaa gtctcgcgcg aatgtggtgc gcaaactcaa taccgacatc
      961 aaaaccgtgg cgaaagccgc agatgagcgg cctgatgcac ccttgattaa aggccgtcgc
     1021 cgccgtggcg tacgccatca gcgacaaacc tgcgccttat tgcaagcaaa ctttggtgtg
     1081 aaccctcgtt aaagagtaaa gatgagcgaa aacgaaacgc ctacattaaa ggctttttcc
     1141 ctattggaat gtttggcggc atcagaccat gcgatgagct tgaccgaatt ggcgcgcatc
     1201 atggacttgc ccaaaccttc gatacaccga catttggcgg ctttggaggc gggtgggctc
     1261 atcattcgtg atgcaggtac caaacacgct tacaccatag gcccacgctt gacccgcttg
     1321 agcagcacag tgttgaacca tggcgatgta cagcgccaac gccatgcgat tctaaaaggc
     1381 ttggtggcag acattggtca aacctgtaac ctgaccatgt tggtgaagtc acaagtcttg
     1441 tatttggacc gcatggagtc accgtcgcca ttgcgtttgg atttgcaacc aggcaccacc
     1501 gtgcctgcgc attcttgtgc cagtggcaag ctttttctat cgaaaatgcc caaagacaaa
     1561 cgccatgcct tgctggagca aaccaagctt gaggcgttga cgcccaacac gctcaccaat
     1621 attgaagaat tggatgcgga attggaacgc atcaatgtgc gcaacgtcag catagacaat
     1681 gaagaatttg tgctcggcat cacctgtatt gcagtaccga tttttgatga taagcaaaat
     1741 tgcatcgccg ccattgccat gcacggccaa gtggcgacca cgccgctcac cgtgtctttg
     1801 tc
//
LOCUS       DAOMVK010000475         1796 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_19170_length_1796_cov_2.372774, whole genome shotgun sequence.
ACCESSION   DAOMVK010000475 DAOMVK010000000
VERSION     DAOMVK010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19170_length_1796_cov_2.372774"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>962
                     /locus_tag="PK856_11785"
     CDS             <1..>962
                     /locus_tag="PK856_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRL03090.1"
                     /translation="TNTDELFENVCYRDLRHINTRSDSEVLLNVFAHELETRVEGDKL
                     TVEHIFDAVTQLHKMVRGAYGVVALIAGYGMVAFRDPYGIRPLILAKQQDEQGRTGYA
                     VSSESVAFNGLDYEVVRDIAPGEAVFISFDGELHSRQCSDASVLKPCLFEYVYFARPD
                     SKIDGVSVYQARADMGVTLAEKVKRELPWEEIDVVMPIPDTSRPSAMELAHHLGINYR
                     EGFIKNRYIGRTFIMPGQAVRKKSVRQKLSPVASEFQGKNVLLVDDSIVRGTTSREIV
                     EMARDAGAKKVYFASAAPEVRYPNVYGIDMPTREELIANGRTVE"
     assembly_gap    963..972
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <973..1249
                     /locus_tag="PK856_11790"
     CDS             <973..1249
                     /locus_tag="PK856_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRL03091.1"
                     /translation="RTVKEIAAEISGDGVVFQDLDELIAAVRKLNPNIQGFDTSCFNG
                     KYLTGDVDEAYLEKLASGKVSAELLKKVNHTLVDHSVRVDDNQDDDE"
     gene            1269..>1796
                     /locus_tag="PK856_11795"
     CDS             1269..>1796
                     /locus_tag="PK856_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRL03092.1"
                     /translation="MSKTWHPETIAIRGGRQISDFSEHSQALYMTSSFTYPTAEDASR
                     LFVGEQAGYTYSRTSNPSIAAFEERMAQLEGAERGLATASGMAAIHATLISLLQNGDH
                     LLSSHSLFGSTSSVVNNMLPRYGIEVTCVSPVDLAAWEAAIQPNTKVLFLETPSNPLG
                     EVADIKALAALAHKHG"
BASE COUNT          450 a          406 c          488 g          442 t
ORIGIN      
        1 tgaccaatac cgatgagttg tttgaaaacg tgtgttaccg cgatttgcgc cacatcaaca
       61 cccgctctga ttccgaagtc ttgttgaatg tgtttgccca tgaattggaa acccgtgtcg
      121 aaggcgacaa actcacggtt gaacacattt ttgatgcagt gacccaattg cacaaaatgg
      181 tgcgtggggc atatggtgtg gtggctttga ttgcaggcta tggcatggtg gcattccgtg
      241 atccgtatgg cattcgtcca ttgattttgg ccaaacaaca agatgaacaa ggtcgcacag
      301 gctatgcagt cagctctgaa tcagtagctt tcaatggctt ggattatgaa gtggtgcgtg
      361 acattgcgcc aggtgaggcg gtgttcatca gttttgatgg tgaattgcac agccgtcaat
      421 gcagtgacgc atcggtgtta aagccttgtt tgtttgagta tgtgtatttt gcccgaccag
      481 actctaaaat tgatggggtg tcggtctacc aagcccgtgc agacatgggt gtgactttgg
      541 ctgaaaaagt caaacgtgaa ttgccatggg aagagataga cgtggtaatg cctatccctg
      601 ataccagccg tcctagcgcg atggagttgg cccaccattt gggcattaac taccgcgaag
      661 gcttcatcaa aaaccgttac ataggccgta cttttattat gcctggccaa gctgtgcgca
      721 aaaaatcggt gcgtcaaaaa ttgagccctg tagcaagcga gtttcaaggt aaaaatgtat
      781 tgttggtgga tgactccatt gtacgcggta ccaccagtcg tgaaatcgtg gaaatggcgc
      841 gtgatgcggg tgctaaaaaa gtgtattttg cttcggctgc gcctgaggtg cgttatccaa
      901 acgtatacgg catcgacatg ccgacgcgtg aagaattgat tgccaatggc cgcactgtgg
      961 aannnnnnnn nnccgcacgg ttaaggaaat cgcagcggag atttcaggcg atggcgtggt
     1021 gttccaagat ttggacgagt tgattgcggc agtgcgcaag cttaatccta acatccaagg
     1081 ctttgatacc tcatgcttta acggcaagta tttgacgggt gatgtggatg aagcgtatct
     1141 tgaaaaactg gccagcggca aggtttctgc tgagctgtta aaaaaagtca atcacacact
     1201 ggtagaccac agtgtgcgcg tagacgacaa tcaagatgac gacgagtgat ttaaaggctt
     1261 aaacacaaat gagtaaaaca tggcaccctg aaaccatcgc catccgcggt ggtcgccaaa
     1321 tcagtgattt cagcgaacac agccaagctt tgtacatgac cagcagcttc acttacccca
     1381 ctgccgaaga cgcatcgcgt ttgtttgtgg gtgagcaagc aggttatacc tacagccgca
     1441 cttcaaaccc aagcatcgcg gcatttgaag agcgcatggc gcaattggaa ggggctgagc
     1501 gcggcttggc cacggccagc ggcatggcgg cgatacacgc taccttaatc agcttattgc
     1561 aaaatggcga ccatttgctc tctagccaca gcttgtttgg ctcgaccagc tcggtggtta
     1621 acaatatgtt gccacgttat ggcattgaag tgacgtgtgt cagccctgtg gatttggcgg
     1681 cttgggaagc ggcgatacag cccaatacca aggtgttgtt tttggaaacc ccatccaatc
     1741 ctttgggtga agtggccgac atcaaagcct tggccgcctt ggcccacaag catggc
//
LOCUS       DAOMVK010000476         1789 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_19276_length_1789_cov_1.856978, whole genome shotgun sequence.
ACCESSION   DAOMVK010000476 DAOMVK010000000
VERSION     DAOMVK010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19276_length_1789_cov_1.856978"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..339)
                     /locus_tag="PK856_11800"
     CDS             complement(133..339)
                     /locus_tag="PK856_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03093.1"
                     /translation="MSNLHSMGNRQAHLVQLFAARIALRQREISSGFLVFTDSGEVKY
                     YEESYSFTVAHLAAFTNMPVYSIG"
     gene            complement(667..861)
                     /locus_tag="PK856_11805"
     CDS             complement(667..861)
                     /locus_tag="PK856_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03094.1"
                     /translation="MLSVSELRQRKQQLEQAIAELIQVFEHETSVEVDSLLTLERSSC
                     TATSQDTQSGHIKVEIHLNM"
     assembly_gap    1007..1025
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1109..>1789)
                     /locus_tag="PK856_11810"
     CDS             complement(1109..>1789)
                     /locus_tag="PK856_11810"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRL03095.1"
                     /translation="WLCHSLSWQTNVRLLCALAEADMQGRVCADKSRALDNIALLREL
                     AREEGCEQTPKVFANEHTRLRYFQGHEVYPDFALQLPQGSKVTLMCGLPASGKNTWVT
                     QHAGGVPVLSYDDTRQRLGLKYGANEGLVAHTVFEMAKTYLREKQDFVWNATHLSAQM
                     RQKNVAVSLAYDAHVRIAYVEADKATLLKRNQARDSSLSNAKLLQMLKHWEMPSVLEA
                     HQLDVFTE"
BASE COUNT          495 a          447 c          360 g          468 t
ORIGIN      
        1 acaagtcatt gacgcaagtc atctggccca caaaaacaat ttcacagcca ttcatcaaaa
       61 tggctgtgaa aagaaaccac atcgcaacga taacaaaaca gcctgttaca ggccgctttg
      121 ctatgccaaa tgtcatccaa tactgtacac gggcatattg gtaaacgcgg ccaaatgtgc
      181 cacggtaaac gaatagcttt cttcgtaata cttcacttca cctgaatcgg tgaacaccaa
      241 aaaaccactg ctgatttcgc gctggcgcaa agcgattctg gcagcaaaca attgcaccaa
      301 atgcgcttgt cgattgccca tgctgtgtaa attagacata ggtttcaatc gcggtgtgct
      361 gatcgcggtt ggatgaaacg gctgaaccag agtcattctg gcgattttca tggcggcatt
      421 catgggcgtg ctctttaaat gtgaaagtaa aatgacaatt agtgtggtta attgccgatt
      481 ataaactttt caatacggtt aggtaatcca ttactcatga gtgacggatt gattttattg
      541 cctatatcta aaattatact caattttagt ttaaatacac ttaaaaaaat aaatctttca
      601 tcgcaagcaa acatgccatc ttaagatgac actgttaagc atctgtttgt caccatgcaa
      661 attcgcttac atattcagat gtatctctac tttaatgtgg ccagactgag tgtcttgcga
      721 agtggctgtg caggagctgc gttcaagcgt gagcaagctg tctacttcaa cagaagtttc
      781 gtgctcgaaa acctgtatca actcagcaat cgcttgctct aactgctgtt tgcgttgacg
      841 caactcagac acactcaaca tcaccatccc tcaccaacat ctaaaaatag attgcaaata
      901 agagccgagt gtgtaccaag agttcattaa tcctgcgaac tttacactta atttaataga
      961 atttaggctc ttacatcaat attcatcaca tcttatttaa acccatnnnn nnnnnnnnnn
     1021 nnnnngaagc ggcctgtaat cgttgttttt taacaacaaa tacaggccgc tgtgttcaca
     1081 ccttcaaaca ataatgggca gaggcctgtt attcagtaaa aacatccaat tggtgcgctt
     1141 ccaatacact gggcatttcc caatgcttga gcatttgcag cagcttggca ttgctcaaac
     1201 tgctgtcacg cgcttggttg cgtttgagca atgtggcttt gtctgcttcc acatacgcaa
     1261 tgcgcacgtg ggcatcgtaa gccaaactca cggccacatt tttttggcgc atttgcgctg
     1321 acaaatgggt ggcattccac acaaaatctt gcttttcgcg caaataagtc ttggccattt
     1381 caaaaacagt gtgtgccacc aagccctcgt tggcaccgta tttcaagccc aagcgctggc
     1441 gtgtgtcatc gtaagacagc acaggcacac ctcccgcatg ctgcgtcacc caagtgtttt
     1501 tgccactggc tggcaagcca cacatcaaag tcaccttgct gccttgcggc aactgcaagg
     1561 caaaatcagg atacacctca tgcccttgga aatagcgcaa gcgcgtgtgc tcattggcaa
     1621 agacttttgg cgtttgctca cagccctctt cacgcgccaa ctctcgcaac aaggcaatgt
     1681 tgtccaaagc ccgactttta tccgcacaca cgcgcccttg catgtcggcc tcggccaagg
     1741 cacacagcaa gcgcacattg gtttgccaag acaggctgtg gcacaacca
//
LOCUS       DAOMVK010000477         1786 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_19318_length_1786_cov_3.166956, whole genome shotgun sequence.
ACCESSION   DAOMVK010000477 DAOMVK010000000
VERSION     DAOMVK010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19318_length_1786_cov_3.166956"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="PK856_11815"
     CDS             <1..807
                     /locus_tag="PK856_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRL03096.1"
                     /translation="LSALDTLQALGVSASIVPDAVFPATLTAYNQGKATKAGSLFEPD
                     VAVLRAKKPDLIIVGGRSKAKQDTVKDLAPTLDLSPNTDHYMADLQARTELLANTFGK
                     QAAAAKRMRTIEIKQAKLKAQTQGKTALMLFTNKGNFMPHAAGERFGFVYDFAGFKSV
                     LPVVPPKDENAPAQPRPEAGSLAAEAAKVKAAERLQAAVNQQPDYLIVLDRGAVNTNE
                     YTAAAEMQKNAILKQAKGKVIMVDANAWYLTGAGLANTELMLDELIKATQ"
     gene            complement(868..1563)
                     /locus_tag="PK856_11820"
     CDS             complement(868..1563)
                     /locus_tag="PK856_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HRL03097.1"
                     /translation="MRTVGLIGGMSWESTVLYYQHINQDIKAALGGLHSAKLVLVSVD
                     FAEIERLQHAGSWHEAGLILAQAAQSLQAAGAECVVVCTNTMHKLAQQIQDAVDIPLL
                     HIGDATARAILNHDIRKVALLGTAFTMEQDFYRARLQAHGLEVLLPNAADRADVHRII
                     YEELCLGEVSPESRARYIAIMLHLVEQGAEAIILGCTEIVMLVGDVDVGVPLFDTTAI
                     HAEAAVAFALGNG"
     gene            complement(1643..>1786)
                     /locus_tag="PK856_11825"
     CDS             complement(1643..>1786)
                     /locus_tag="PK856_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HRL03098.1"
                     /translation="YPQVPPKVEYSLTEFGKTLMPVIDVMHAWGEQYAGECAKHRKSS
                     QAE"
BASE COUNT          444 a          522 c          441 g          379 t
ORIGIN      
        1 ttgtcggctt tagacacttt gcaagccttg ggtgtgagcg ccagcatcgt gcccgatgcc
       61 gtgtttcctg ccactttgac cgcttacaac caaggcaaag ccaccaaagc aggcagcttg
      121 tttgagcccg atgtggccgt gttacgtgcg aaaaaacccg atttaatcat cgtgggcggc
      181 cgctccaaag ccaaacaaga tacggtaaaa gaccttgccc cgactttgga tttgagcccc
      241 aacaccgacc actacatggc cgatttgcaa gcgcgcaccg agttactcgc caacactttt
      301 ggcaaacagg ccgcagccgc caaacgcatg cgcaccatcg aaatcaagca agccaagctc
      361 aaagcgcaaa cccaaggcaa aaccgcgctg atgctgttca ccaacaaagg caatttcatg
      421 ccacatgccg caggcgaacg ctttggcttt gtgtatgatt tcgcaggctt taaatcggtg
      481 ttgcccgtgg tgccacccaa agacgaaaac gcgcccgcac agccgcgccc tgaagcaggc
      541 tctcttgcag ccgaagccgc caaagtcaaa gccgccgagc gtttacaggc cgctgtgaac
      601 caacagcctg attacctcat cgtgctcgac cgcggcgcgg tcaacaccaa tgaatacacc
      661 gccgcggccg aaatgcagaa aaacgcgatt ttgaaacagg ccaaaggcaa agtcatcatg
      721 gtcgatgcca acgcttggta tttgacaggc gctggcttgg ccaacaccga attgatgttg
      781 gatgaattga ttaaagccac ccaataagtc tttgtgcatg aacaagcggc ctgtaaatcg
      841 tttacaggcc gcttttgtgt atagaaacta gccattgccc aaggcaaacg ccacagcggc
      901 ttcggcatgg atggcggtgg tgtcgaacag tggcacgccc acgtccacat cacctaccaa
      961 catcacgatt tcggtacagc ccaagataat cgcctctgcg ccttgttcga ccaagtgcaa
     1021 catgatggca atgtaacggg cgcgcgactc ggggctgact tcgcccaaac acaattcttc
     1081 ataaataata cggtggacat cggcgcggtc tgccgcgttt ggtagcaaca cttccaaacc
     1141 atgcgcttgc aaacgggcgc ggtaaaaatc ttgctccatg gtgaaagcgg tgccaagcaa
     1201 tgcgactttg cggatgtcgt ggttcagaat ggcgcgcgca gtggcatcgc caatgtgcaa
     1261 taagggaatg tctaccgcgt cttggatttg ctgcgccaat ttgtgcatgg tattggtgca
     1321 caccaccacg cactcggccc cagcggcctg taagctttgc gccgcttgcg ccaaaatcaa
     1381 acctgcttca tgccatgaac ctgcgtgttg caagcgctcg atttcggcaa aatccacgct
     1441 caccaacacc agcttcgccg aatgcaaacc acccaatgcg gctttgatgt cttggttgat
     1501 gtgttggtaa tacaagacgg ttgattccca cgacatgccg cctatcaagc ccactgttcg
     1561 catagccaca ctcctcgttg attggtgtta cccatcttaa tcagtgaaca aaccgcggtc
     1621 tagaacaaag ttgcgccagc catcactctg cttgcgatga cttgcggtgc ttggcgcatt
     1681 ctcctgcata ttgctcgccc caagcatgca tcacatcaat gacgggcatc agggttttgc
     1741 caaactcagt aagcgaatac tccaccttgg gcggcacttg tggata
//
LOCUS       DAOMVK010000478         1783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_19358_length_1783_cov_4.700231, whole genome shotgun sequence.
ACCESSION   DAOMVK010000478 DAOMVK010000000
VERSION     DAOMVK010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19358_length_1783_cov_4.700231"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..1443)
                     /gene="fabF"
                     /locus_tag="PK856_11830"
     CDS             complement(199..1443)
                     /gene="fabF"
                     /locus_tag="PK856_11830"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003824919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRL03099.1"
                     /translation="MSKKRIVITGLGMVSPLGNTVADNWNNLLAGVSGIGPITRFDAS
                     NLACQIAGEVKDFDITQYIPAKEARRMDVFIHYGIAAAFQAIEDAGLDNIEGLDKTRV
                     GVNIGAGIGGLPSIEATGADVIAGGIRKINPFFIPGSLINLIAGHVTIMKGYQGPSYG
                     IVSACTTGAHCIGDAARMIAYGDADIMIAGGAEGAICTLGMGGFAAMKALSTRNDEPQ
                     KASRPWDKERDGFVMGEGAGVMVLESYDSAIARGANIYAELVGFGMSSDAHHITAPTI
                     DGPALAINRALKDAGLEPTDIDYVNAHGTSTPLGDANETNALKTAFGDHAYKLVVNST
                     KSMTGHLLGGAGGIEAVITALAIKNQIAPPTINIDDQDIEAGCDLDYNKEGARPMNIR
                     VAISNSFGFGGTNGTVVLKRVD"
     gene            complement(1525..1758)
                     /gene="acpP"
                     /locus_tag="PK856_11835"
     CDS             complement(1525..1758)
                     /gene="acpP"
                     /locus_tag="PK856_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRL03100.1"
                     /translation="MENIEQRVKKIVAEQLGVNEAEVKNESSFQEDLGADSLDIVELV
                     MALEEAFGTEIPDEEAENITTVQQAIDFLSAAE"
BASE COUNT          503 a          473 c          354 g          453 t
ORIGIN      
        1 aaagttgcag attaaaacag tccaagttgt cgttgtaagg caacctgcac ccatcaaaaa
       61 aaacagacca taactttaac acgtttagtc agcactgact aagcctctgc tgccatttat
      121 tttccaaagc ctgagcctca gaaaaaacaa agcggcctgt aaagtttaca ggccgcttgt
      181 ggataccgaa ttatgccatc agtccacgcg tttcaacacc accgtaccat tggtaccacc
      241 aaaaccaaac gagttggaaa tggccacacg aatgttcatt ggacgggcac cttctttgtt
      301 gtagtccaaa tcacaacccg cttcaatgtc ttggtcatca atgttgatgg taggcggtgc
      361 gatttgattt ttaatcgcca aagcggtaat caccgcttca ataccacccg caccacccaa
      421 taagtgacct gtcattgact tggttgaatt caccaccaat ttgtaagcat gatcgccaaa
      481 tgccgttttc agtgcattgg tttcattggc atcgcccaaa ggcgttgaag taccgtgtgc
      541 attcacataa tcaatgtctg ttggctctaa gcccgcatct ttcaatgcgc ggttaatcgc
      601 caaagcaggg ccatcaatgg taggtgcggt aatgtgatgt gcatcagaac tcatgccaaa
      661 acccaccagc tcagcataaa tattggcacc acgcgcaatg gctgaatcat aagattccaa
      721 taccatcaca ccagcgccct cacccatcac gaaaccatca cgctctttgt cccaaggacg
      781 agaggctttt tgtggctcat cgttgcgggt agacaaggct ttcattgccg caaaaccacc
      841 catacccaag gtgcaaatcg caccctcagc accacctgca atcatgatat cggcatcgcc
      901 gtaagcaatc atgcgcgcag catcgccaat acagtgtgca cccgtggtac aggccgacac
      961 gatgccataa cttgggcctt ggtagccttt catgatggtt acgtgaccag caatcagatt
     1021 aatcaacgag ccagggatga aaaatggatt gattttgcgt atgccgccag caatcacatc
     1081 agcaccagtg gcctcaattg aaggtaagcc acctatgccc gcaccaatgt tgacaccaac
     1141 acgtgttttg tccaagcctt caatattgtc caaaccagca tcttcaatcg cttgaaacgc
     1201 tgcagcaata ccataatgaa taaacacatc catacgacgg gcttctttgg ccgggatgta
     1261 ttgggtgatg tcaaagtcct tcacctcacc cgcaatttga cacgccaaat tggaagcatc
     1321 aaaacgggtg attggaccaa taccactgac accagctaat aaattgttcc agttatcagc
     1381 aaccgtattg cccaaaggcg acaccatgcc taaaccagtt atgactatcc tttttttact
     1441 cattcgtgtg gaacccatag taaaaacctc tgcacagcca acgagtgcgc tttgcagagg
     1501 ttaaagtatt gcgacagaga aaaattactc agcagctgac aagaagtcga ttgcttgttg
     1561 tactgtggtg atgttttcag cttcttcgtc agggatttca gtgccgaaag cttcttccaa
     1621 agccataacc aattcaacga tgtccaaaga gtcagcgccc aagtcttctt ggaatgaaga
     1681 ttcgtttttc acttcagctt cattcacacc caattgttca gcaacaattt tctttacgcg
     1741 ctgttcgata ttttccatgt tacttccttt accttgatct taa
//
LOCUS       DAOMVK010000479         1782 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_19372_length_1782_cov_3.539664, whole genome shotgun sequence.
ACCESSION   DAOMVK010000479 DAOMVK010000000
VERSION     DAOMVK010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19372_length_1782_cov_3.539664"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="PK856_11840"
     CDS             complement(<1..544)
                     /locus_tag="PK856_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRL03101.1"
                     /translation="MDFRFDIIYEYREMFWTGAKYTLGLTAFAVAVGTVLGLIGALCR
                     LANFEKGNIVLRALGWVLRTVSLLYVTLFRGTPLFVQIFIWHFIWSVALINPADGWII
                     SGDLARELRKEYGALIAGALALSTNAGAYITEIFRAGIQSIDRGQVEAARSLGLNYAQ
                     AMRHVILPQATRRMLPPFGNE"
     gene            782..1714
                     /gene="ribF"
                     /locus_tag="PK856_11845"
     CDS             782..1714
                     /gene="ribF"
                     /locus_tag="PK856_11845"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HRL03102.1"
                     /translation="MQIWLGQTDANHFLNGCALTIGNFDGVHLGHQHVLARLQQEADA
                     RGLPAVVMVFEPQPKEYFSRLFGKDLPPRLSPLRDKMRLISKIHPHIKGVNVRRFNSE
                     FAAIAAQSFIDEVLIAQLNVKYLLIGDDFCFGAKRQGNFALLQQQTAFVTENTSSLMV
                     ENTRASSTVIREALGRGDLDLAERLLGHPYVLSGHVKHGAKLGRTLGCPTANVHLPPL
                     MFPLSGVYVVEVMGSFGKRRGVASFGLNPTVTDSRNQKCEVHLFDWHESLYGQRIQVA
                     FKHKLRDEWKFDSLEALQHQIHLDMSQARAWDEA"
BASE COUNT          485 a          399 c          451 g          447 t
ORIGIN      
        1 attcattgcc aaatggaggc agcatgcggc gggtggcttg cggcaaaatc acatggcgca
       61 tggcttgggc ataattcaaa cccaaagaac gagcagcttc cacttgacca cggtctatgg
      121 actgaatgcc cgcgcggaag atttcggtga tgtaagcgcc tgcattggta gacaaggcca
      181 aagcgccagc aattaatgct ccgtattctt tgcgcagctc gcgtgccaaa tcgccagaga
      241 tgatccaacc atcggcagga ttgatcaaag caaccgacca aataaagtgc cagatgaaga
      301 tttgtacaaa caaaggggtg ccacggaaca gagtgacgta cagcaaggag acggtgcgca
      361 aaacccaacc caaggcgcgc aacacgatgt tgcctttttc aaagttggcc aaacgacaca
      421 atgcgccgat taaacccaat actgtaccta cggcaactgc aaatgcggtc agacccaaag
      481 tgtatttggc acccgtccaa aacatttcgc gatattcgta gatgatatca aagcgaaaat
      541 ccatatactc actccaactt aaaggatgtg taccagaggc tacaactgtg tgtgattggt
      601 caatttagcc acatgaaacg ttaatgtagg gcgaagttta agtcaaagcg gtgcatgatg
      661 ctaccgattt taacgataga ttgttgtgct gtgcactaat acagtgcatg cttacactag
      721 ctgcaaacgc acttagcgtt taaaatataa cgttttatca gtgtaaagat actgtattaa
      781 tatgcaaatt tggttgggtc aaaccgatgc aaaccatttt ctcaatggtt gtgcgttaac
      841 aatcggcaac tttgatgggg tgcatttggg gcaccaacat gtattggcgc gcttacaaca
      901 agaagccgat gcgcggggct tgcctgcggt ggtgatggtg tttgagccgc aaccaaaaga
      961 gtatttctca cgcttgtttg gcaaggattt gccaccgcgc ttgagtcctt tgcgcgataa
     1021 aatgcgttta atttccaaga ttcatcccca tattaaaggg gtgaatgtgc gacgctttaa
     1081 cagtgagttt gccgcgattg ctgcacaaag ttttattgat gaggtgctca ttgcccagtt
     1141 gaatgtgaag tatttgctga taggcgatga tttttgtttc ggggccaagc gccaaggtaa
     1201 ttttgccttg cttcagcagc aaacagcctt cgtcactgaa aatacttcca gcctcatggt
     1261 ggaaaacacc cgcgcatcca gtaccgtcat tcgtgaagcc ttgggccgcg gtgatttgga
     1321 tttggctgag cgcttgctgg gccatcctta tgtcttgtct ggccatgtga aacacggggc
     1381 caaattggga cgtactttgg gttgcccgac ggccaatgtg catctgccac cattgatgtt
     1441 tccgttaagt ggcgtgtatg tggttgaagt gatgggcagc tttggcaagc gtcgcggtgt
     1501 ggccagtttt ggactcaatc ccactgtaac cgacagccgc aatcaaaaat gtgaagtgca
     1561 tttgtttgat tggcatgaat cgctgtatgg acagcgcata caagtggcgt tcaaacacaa
     1621 attgcgcgat gaatggaagt ttgacagctt ggaagcattg caacaccaaa tacacttaga
     1681 catgtctcaa gcgcgtgctt gggacgaggc ctaaagttta caggccactt aatttagtaa
     1741 agaccaacga tagagaatgt tatgaccgat taccgaacca cc
//
LOCUS       DAOMVK010000480         1781 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_19392_length_1781_cov_3.035921, whole genome shotgun sequence.
ACCESSION   DAOMVK010000480 DAOMVK010000000
VERSION     DAOMVK010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19392_length_1781_cov_3.035921"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="PK856_11850"
     CDS             complement(<1..821)
                     /locus_tag="PK856_11850"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HRL03103.1"
                     /translation="MYDQIFDWLKENRITEVECILPDITGVARGKILPKDKFISEPEM
                     RLPEAVLLQTVTGEFPADEMLDLTDPDMELRPDPDSLRVVPWAVDPTAQLIFDCFSPD
                     GAPVETAPRNVLRRVLKLYEELGYTPVVAPEMEFYLISPNPDPDIPLIPPVGRTGRSE
                     FGRRSFSIDAVNEFDPLFEEIYDYCHAQNLEVDTLIHEVGAAQMEINFLHGDALDLAD
                     QVFLFKRTVREAAFHHKMYATFMAKPMEGEPGSAMHIHQSLTDNKTGKNAFSNDDG"
     gene            complement(898..1659)
                     /locus_tag="PK856_11855"
     CDS             complement(898..1659)
                     /locus_tag="PK856_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HRL03104.1"
                     /translation="MRRPVIGIPCDVKFVGRADFHAVGEKYIAAVQGAVGDVVLLPAW
                     GDMNILKRLLPGLDGIFLTGSLSNVKPSRYGSDLADPDTLLDEKRDNTTLPLIEHVLD
                     IGMPLFGVCRGFQEMNVALGGTLHQKVQEVPGMMDHREPGDTIDSNILYADAHTISLA
                     ENSLLKQWMGGANETMVNSLHQQGVKDLASGLTAEAHAPDGLIEAFRVTDAKTFAYAV
                     QWHPEWLYTEKAASIALFKAFHEACVEYAAATAGR"
BASE COUNT          424 a          433 c          461 g          463 t
ORIGIN      
        1 ccatcgtcat tgctgaaggc atttttgcct gttttgttgt cggtcaagct ttggtggatg
       61 tgcatggcgc tgccaggctc accttccatc ggtttggcca tgaaggttgc gtacattttg
      121 tggtggaacg cagcttcacg cacggtgcgc ttgaacaaga acacttggtc ggccaagtcc
      181 aaggcatcac catgcaagaa gttaatctcc atttgtgctg cgcccacttc gtgaatcaaa
      241 gtgtccactt ccaggttttg tgcgtggcaa tagtcgtaga tttcttcaaa caaagggtcg
      301 aattcattca ctgcatcaat agagaaagag cggcgaccaa attcagagcg gcctgtacga
      361 cccacaggtg gaatcaatgg aatgtctggg tctgggttgg gtgagatgag gtaaaactcc
      421 atctcaggtg cgaccacagg ggtgtagccc aattcttcat acagtttcaa cacgcggcgc
      481 aaaacattac gcggggcggt ttcaacaggt gcgccatctg gtgagaagca atcaaaaatc
      541 aattgtgctg tcgggtctac cgcccaaggc accacgcgca gactgtctgg gtctgggcgc
      601 aattccatgt cggggtcggt taagtccagc atttcatcag caggaaattc acctgtcact
      661 gtttgcaaca ataccgcttc gggcaagcgc atttcaggct cggagatgaa tttgtctttg
      721 gggaggattt tgccgcgagc gacaccggtg atgtcaggca aaatacattc aacttcagta
      781 atgcggtttt ctttaagcca atcaaatatt tggtcgtaca taagtcgtcc ttggtttaca
      841 ttagcgtgat tccctttgaa tcaaggcctg tttacaggct tttgggttgc aaaatcgtta
      901 gcggccagct gtggcagccg cgtattccac acaagcttca tgaaatgctt tgaataaggc
      961 gatggaggcg gctttttcgg tgtacagcca ttctggatgc cattgcacgg cataggcaaa
     1021 cgtcttggca tcggtgacac ggaaagcttc aatcaagccg tcgggcgcat gggcttcagc
     1081 cgtaagccct gatgccaagt ctttcacacc ttgttggtgc aaagagttga ccatggtttc
     1141 attagcgccg cccatccatt gtttgagcaa actgttttcg gccaagctga tggtgtgggc
     1201 atcagcgtac agaatattgg aatcgatggt gtcgccaggt tcacggtggt ccatcatgcc
     1261 aggcacttct tgtacctttt gatgcagcgt gccacccaaa gccacgttca tttcttggaa
     1321 gccgcggcac acgccaaaca gaggcatacc aatgtccagc acatgctcaa tcaagggcaa
     1381 agtggtgttg tcgcgctttt catctaataa agtatcagga tcggccaaat cgctgccgta
     1441 gcggctgggt ttgacattgg ataaagagcc agttaagaaa atgccatcta agcctggtaa
     1501 caggcgtttg aggatgttca tgtcgcccca agcggggagt aacaccacat cacctactgc
     1561 gccttgtacg gcggcaatgt atttttcacc tactgcatga aagtcggcac ggccaacaaa
     1621 tttgacatca caagggatgc cgatgacagg tctacgcatt cagcgctcct aaattgtttt
     1681 tccttatgcc gtgggtcttg gacgctggat tacaagatac ggtgcatctt gcttaccaaa
     1741 cacggcatat atgtgttttt ctactatccc atgccgcctg a
//
LOCUS       DAOMVK010000481         1780 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_19407_length_1780_cov_3.151884, whole genome shotgun sequence.
ACCESSION   DAOMVK010000481 DAOMVK010000000
VERSION     DAOMVK010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19407_length_1780_cov_3.151884"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="PK856_11860"
     CDS             <1..710
                     /locus_tag="PK856_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HRL03105.1"
                     /translation="INHPLLDCFNAYGTWLFWPLGEQAIMWGNMFVIDPLFTLPMLFG
                     FVWLAIRPVGKHSGKVMYGAIGLSVLYFAWSLAAQMWVMQKVDKQLADLGLQDAPRLV
                     TATPFNTLVWQVLVQVPDGVLSGSHSLTQDEADAPIRLQHIGSDTAALAKVQDNVALQ
                     RLRRFNQGYFVAREVDGKLIISDVRMGREPHYTFNFAIAKWQNGQWQALTPPEQIQDR
                     PDLKQEWAYLQKRLWGG"
     assembly_gap    905..914
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<915..1259)
                     /locus_tag="PK856_11865"
     CDS             complement(<915..1259)
                     /locus_tag="PK856_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03106.1"
                     /translation="MMKTMMTVLLAAALSLPMLAGAEGLQFKRSNDNGYDDYSGQITV
                     SGKYSRHFDDEVMGDSVCFDVNGPTAKLIPRKKGDERNTWFCFRNQQTAVNTFKIAKK
                     PGNRHCGYEGTAT"
     gene            1444..1761
                     /locus_tag="PK856_11870"
     CDS             1444..1761
                     /locus_tag="PK856_11870"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HRL03107.1"
                     /translation="MITVIAEIQVRSGDGHKQKVLNAFSKLIPTVLQEDGCHGYRLLL
                     DYEGNVDYQSLDENMITMLEHWTDIAALNAHLQSAHMQAYAQEVADDVVGMKVKILTE
                     GLA"
BASE COUNT          425 a          387 c          473 g          485 t
ORIGIN      
        1 tgattaacca ccctttgctc gattgtttca atgcctatgg cacatggctg ttttggcctt
       61 tgggcgagca agccatcatg tggggcaata tgtttgtcat cgatcctttg tttaccttgc
      121 caatgctatt tgggtttgtt tggcttgcca tacggcctgt gggtaaacac agtggcaagg
      181 tgatgtatgg tgcgattggt ttgagtgtgt tgtatttcgc gtggtcgctg gcggcgcaaa
      241 tgtgggtgat gcaaaaagtg gacaagcagt tggccgattt gggtttgcaa gacgcgccac
      301 gcttggtcac tgccacgcct ttcaacactt tggtgtggca ggtattggtg caagtgccag
      361 atggggtgtt gagcggcagc cattctctca ctcaggacga ggccgatgcg ccgatacgtt
      421 tgcaacacat aggcagcgat accgctgctt tggctaaggt gcaagacaat gtggcattac
      481 agcgcttgcg ccgtttcaat caaggctatt ttgttgcgcg ggaagtggat ggcaagctca
      541 tcatcagcga tgtgcgcatg ggacgcgagc cgcattacac tttcaatttt gccatcgcca
      601 aatggcaaaa cggccaatgg caagccttga cgccacccga gcaaatccaa gaccgtcctg
      661 atctcaagca agaatgggct tatttgcaaa aacggctgtg gggcgggtag gtgatgcccg
      721 atagttgaat ctgattttta cgctgtatta cccatgtcaa agcggcctgt aaattgttta
      781 caggccgctt atttgatgga attgaggcat cacccttcgc tgtaacactg ggtatttttc
      841 ggtgccgaca catttttggc gctgatcaat tgggccaaat ctgcgccatc actgatttcg
      901 gtgtnnnnnn nnnnggtagc tgtgccttca tagccacaat ggcgattgcc tggttttttg
      961 gcaattttga aggtgttgac ggcagtttgc tggttgcgaa agcaaaacca agtgttgcgc
     1021 tcatcgcctt ttttgcgtgg tatcaatttg gcagtggggc cattaacatc aaagcacaca
     1081 ctgtcaccca tcacttcatc atcaaaatgg cgcgaatact tgccgctgac ggtgatttgg
     1141 ccagagtaat cgtcatagcc attgtcattg ctgcgtttga attgcaagcc ttccgcaccc
     1201 gccaacatgg gcaggcttaa agcggcagca agcaaaacag tcatcatggt tttcatcatc
     1261 cactccttaa gtcatggttg tggcacatag taaaggcagt ctaccgactt tggcaatttg
     1321 aatgggctct caataggagg gacttacaca gtggcatgct ttcaaacaga gtttgttgtc
     1381 catttacagt tgctgaggcc ttcggttaca gtagaataaa tacgccaact taaggagaaa
     1441 tatgtgatta cagtcatagc agagattcaa gtgcgttcag gtgatgggca caaacaaaaa
     1501 gttctcaatg catttagcaa gctcattccc actgttttgc aagaagacgg atgccacggc
     1561 tatcggttac tgctggatta tgagggcaat gtggattatc aaagtctgga tgagaacatg
     1621 attaccatgc ttgagcactg gacggatatt gcagcgttga atgcccattt acaatctgcg
     1681 cacatgcaag cgtatgcaca agaagtggct gatgatgtgg tggggatgaa agtgaaaatc
     1741 ctgaccgagg gcttggcata atttggctca tcactgatgt
//
LOCUS       DAOMVK010000482         1779 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_19424_length_1779_cov_2.544664, whole genome shotgun sequence.
ACCESSION   DAOMVK010000482 DAOMVK010000000
VERSION     DAOMVK010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19424_length_1779_cov_2.544664"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..>1779)
                     /locus_tag="PK856_11875"
     CDS             complement(167..>1779)
                     /locus_tag="PK856_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009116648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRL03108.1"
                     /translation="EGLAYQIVHRQVPENLSNAIVFSLDMGALLAGTKYRGDFEARIK
                     AVLKELTELPNAILFVDEIHTIIGAGSVSGGTMDASNLLKPALAKGQLRCIGATTYQE
                     YRTIFDKDHALSRRFQKVDVGEPSAAETVAILRGLKSSFESFHHVKYTQEALEAAVEL
                     SVRYINERFLPDKAIDVIDEAGAAQKVKPKSKQRKTIAREQIEEIVAKMARIPETTVS
                     TDDKNVLKNLNRTLKSMVFGQDKAIEALVNAIKMSRSGLGQPEKPIGSFLFSGPTGVG
                     KTEVSKQLAFSLGVPFIRFDMSEYMERHAVSRLIGAPPGYVGYEEGGLLTEAISKQPY
                     SVLLLDEIEKAHPDIFNVLLQVMDHGTLTDNNGRKADFRNVVIIMTTNAGAESLAKPS
                     IGFANSKASGDEMGAINKLFSPEFRNRLDAIVSFASLDENIIQQVVDKFLLQLEQQLL
                     AKQVEIEFTDGLKAYLAEKGFDPLMGARPMSRLIQTTIRKALADELLFGKLAEGGRVT
                     VDWDNKLGDVQLIFPTAKSDRELAQACE"
BASE COUNT          463 a          479 c          379 g          458 t
ORIGIN      
        1 aacatgtggc cgatgctgaa gccaacgctg gcaaacaccc caatgtggat ttgctactgg
       61 ccgtacaagc cgaacaaaag caataccatt tacaagccat cgcccaataa gcggcctgta
      121 aaaccaaaaa accgccttac ggcggttttt ttattgctca catctgttat tcacaggctt
      181 gcgccaattc tcggtcgctt ttggcggtag ggaatatcaa ttgcacatca cccaatttgt
      241 tgtcccaatc aacggtaaca cgtccgcctt cagccaactt accaaacaac aactcatccg
      301 ccaatgcttt gcgtatggtt gtttgtatca agcgactcat cgggcgcgca cccatcaatg
      361 ggtcgaagcc tttttcagcc aaataggctt ttaagccatc ggtaaactca atttcgactt
      421 gtttggccaa caattgctgc tccaattgca acaagaattt gtccaccact tgctgaatga
      481 tgttctcatc caaagaagca aacgacacga tggcatccaa acggttgcgg aattcagggc
      541 tgaacaactt gttaatcgcc cccatttcat ccccagaagc cttggaattg gcaaaaccaa
      601 tactcggctt ggccaagctt tccgcccctg cattggtggt catgataatc accacattgc
      661 gaaaatcggc tttgcgacca ttgttatcgg ttaacgtacc gtgatccatt acctgcaaca
      721 acacattgaa aatatcggga tgggctttct caatctcgtc caacaacaat acagaatacg
      781 gttgcttgct gattgcctcg gtcaataagc cgccttcttc atagcccaca tagcccggag
      841 gcgcaccaat caagcgcgat accgcatggc gctccatgta ttcagacatg tcaaaacgta
      901 tgaacggcac acccagtgaa aaggccaatt gcttagacac ttcggtttta cccacccctg
      961 tcgggccaga gaacaagaaa ctgccgatgg gtttttcggg ctgccccaaa ccagagcgcg
     1021 acattttaat cgcattcacc aaggcttcaa tggctttgtc ttggccaaac accatcgatt
     1081 tcaaagtacg gttgaggttt ttcaacacat ttttgtcatc ggtagacacg gtagtttctg
     1141 gaatgcgcgc cattttcgcg acaatttctt caatttgttc acgggcaatg gttttgcgct
     1201 gtttggattt gggtttcact ttttgcgcag cacccgcttc atcaatcaca tcaatcgctt
     1261 tgtctggtaa gaagcgctca ttgatgtagc gcaccgacaa ttccacagcg gcttccaaag
     1321 cctcttgcgt gtatttcaca tggtggaagc tttcaaacga agacttcaaa ccacgcaaaa
     1381 tcgccacggt ttctgccgcg cttggttcac ccacatccac tttttggaag cgacggctca
     1441 aagcatggtc tttgtcaaaa atggtgcggt attcttggta agtggtggcg ccgatgcagc
     1501 gcaactgacc tttggccaaa gccggtttca acaagttgga agcatccatg gtgccaccag
     1561 acacactgcc tgcaccgatg atggtgtgga tttcgtccac aaacaaaatg gcattgggca
     1621 attcagtcaa ctctttcaaa accgctttga tgcgcgcctc aaaatctcca cggtatttgg
     1681 taccggccag taaagccccc atgtctaaag aaaacacgat ggcattgctg aggttttcag
     1741 gcacttgtct gtgcacaatt tgataagcca aaccttctg
//
LOCUS       DAOMVK010000483         1779 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_19426_length_1779_cov_2.370650, whole genome shotgun sequence.
ACCESSION   DAOMVK010000483 DAOMVK010000000
VERSION     DAOMVK010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19426_length_1779_cov_2.370650"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /gene="lepA"
                     /locus_tag="PK856_11880"
     CDS             <1..1254
                     /gene="lepA"
                     /locus_tag="PK856_11880"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRL03109.1"
                     /translation="PPPMGDVDAPLKALIVDSWFDNYVGVVMLIRVVDGVLKLKDKVK
                     FMSTKSETLVEQLGVFTPKSVAKQQLSAGEVGFLITGIKELQAAKVGDTVTLAAKPAE
                     QALPGFKEVQSQVFAGLYPVESHDYDALRDALEKLQLNDASLKFEPEVSQALGFGFRC
                     GFLGLLHLEIVQERLEREFDMDLITTAPTVIYEVLMKDGKKIEVENPSKLPDIGTIET
                     IFEPIITATILVPQEYVGSVMTLCNVKRGIQKNMQYMGRQVMLTYDLPMNEVVMDFFD
                     KLKSTSRGYASLDSEFKEFQAADLIKLDIMVNGEKVDALSLIVHRSNSVYRGRELASK
                     MRELIPRQMFDIAVQASIGNQIIARETVKALRKNVLAKCYGGDISRKKKLLEKQKAGK
                     RRMKQVGNVEIPQSAFLAILQVGDK"
     gene            1258..>1779
                     /locus_tag="PK856_11885"
     CDS             1258..>1779
                     /locus_tag="PK856_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HRL03110.1"
                     /translation="MSENLLYMAIAAFVIGVVLFAISSKQRQDTGEWSNTLQWGYLLM
                     MVGVFGVLAKYLSFTAVLLIFVLITGMIWGISKMVRSKAKQDLAGRKLPEDKEARKIL
                     QQDAYEKRVGHFVDYMSGFFPIILIVFVLRTFVAEPFQIPSSSMRPGLVVGDFILVNK
                     FNYGIRMPIANNVV"
BASE COUNT          466 a          338 c          477 g          498 t
ORIGIN      
        1 ccaccaccaa tgggcgatgt tgatgcacct ttgaaagcct tgattgtgga ctcatggttt
       61 gacaattatg tgggcgtggt gatgttgata cgtgtggttg atggcgtgtt gaagctcaaa
      121 gacaaagtca aattcatgtc taccaagtct gaaaccttgg ttgaacaatt gggtgtgttc
      181 acgccaaaat cggtggcgaa acagcaattg tctgcgggtg aagtaggctt cttgattaca
      241 ggcattaaag agttacaggc cgctaaagtg ggcgataccg tgactttggc ggctaaacct
      301 gctgagcaag cattgcctgg atttaaagag gtgcagtcac aggtgtttgc cggcctgtat
      361 cctgtggaaa gccacgatta cgatgctttg cgagatgcct tagaaaagct gcaattgaac
      421 gatgcttcgt tgaaatttga accagaggtg tcacaagctt tgggttttgg cttccgttgt
      481 ggcttcttgg gcttgttgca cttggagatt gtgcaagagc gtttggaacg cgaatttgac
      541 atggatttga ttaccacggc accgacggta atttatgaag tgttgatgaa agatggcaag
      601 aaaattgagg tggaaaaccc atctaaattg ccagacattg gtacgataga aaccatattt
      661 gaacccatca tcaccgccac cattttggtg ccgcaagagt atgtgggctc ggtcatgact
      721 ttgtgtaatg tcaaacgagg catacaaaag aacatgcaat acatgggtcg ccaagtgatg
      781 ctgacttatg atttgcctat gaacgaagtg gtgatggatt tctttgacaa attgaaatct
      841 acttcacgtg gttatgcctc attggattct gaattcaaag aattccaagc cgccgatttg
      901 attaagctcg acatcatggt gaatggtgaa aaagtggatg cattgagctt gatcgtgcat
      961 cgcagcaatt cggtttaccg cggtcgtgag ttggcatcga aaatgcgtga actgattccg
     1021 cgtcagatgt ttgacattgc ggtacaggcc tctattggca atcaaattat tgcccgtgaa
     1081 accgtgaaag ccttgcgcaa aaacgtgttg gccaaatgtt atggtggtga catttcacgt
     1141 aagaaaaaac tgttggaaaa acaaaaagcc ggtaagcgcc gcatgaagca agtgggcaac
     1201 gtggaaattc cacagtcggc gttcttggcg atattgcaag ttggagacaa gtaattcatg
     1261 agtgaaaatt tgctgtacat ggcgatagcc gcgtttgtca ttggtgtggt gttgtttgct
     1321 atcagcagta aacagcgtca agacacgggt gagtggtcta acaccttgca atggggttat
     1381 ttgttgatga tggtgggcgt atttggggta ttggccaaat atttgtcttt caccgcggtt
     1441 ttgctgatat ttgtcctgat cacaggcatg atttggggca tcagtaaaat ggtgcgcagc
     1501 aaggccaagc aagatttggc tgggcgaaag ttgcctgaag acaaagaggc ccgcaagatt
     1561 ttacagcaag acgcttatga aaaacgtgtc ggacattttg tcgattacat gagtggtttc
     1621 ttccccatta tcttaatcgt gtttgtgctc agaacttttg tggcagagcc tttccaaatt
     1681 ccttccagct ccatgcgacc tggcttggtg gtaggcgatt ttatcttggt gaacaaattc
     1741 aattacggca tccgcatgcc gatcgccaac aatgtggtg
//
LOCUS       DAOMVK010000484         1776 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_19468_length_1776_cov_3.762347, whole genome shotgun sequence.
ACCESSION   DAOMVK010000484 DAOMVK010000000
VERSION     DAOMVK010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19468_length_1776_cov_3.762347"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            363..1259
                     /gene="rlmF"
                     /locus_tag="PK856_11890"
     CDS             363..1259
                     /gene="rlmF"
                     /locus_tag="PK856_11890"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HRL03111.1"
                     /translation="MKPTIPPSSLHPNNPHQGRYDLDALAVAVPALQSFFITTPRGDT
                     SIDFSNAKAVMCLNQALLKHFYGMEAWSVPQGFLCPPIPGRADYVHHVADLLVKRPVS
                     RQHQAVRMLDIGTGANLIYPIIATRALGWQVVGSDINATALAYAQKLIDANPVLAGTT
                     LRLQSDAGKVFTGVIQADEYFDVTLCNPPFFKSADEANKQAARKWRNLKGAKASSVRN
                     FGGQNAELWCEGGELAFLRRLIAESHLFAQQVGWFTSLVSNQDHLPKLQQALRKLHAA
                     EIKVMAMTLGQKRSHVLAWRFE"
     gene            1374..1592
                     /locus_tag="PK856_11895"
     CDS             1374..1592
                     /locus_tag="PK856_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03112.1"
                     /translation="MSGFDLGLVLYVLYTALTDWWVFRHNVAPDTDRISLLRQCGGSG
                     DKMAAIIVWALVTLSLGFAVIEAWLWTD"
     gene            complement(1589..>1776)
                     /locus_tag="PK856_11900"
     CDS             complement(1589..>1776)
                     /locus_tag="PK856_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03113.1"
                     /translation="LMYVYLIRSIGVVKGSYMVAFFPAIGGTASVLLGETPLSLNLVL
                     GIGLAMVGAMIALLKRA"
BASE COUNT          436 a          513 c          425 g          402 t
ORIGIN      
        1 acaaaatgtc tatcatgcca gcgagattga cttggaagcg gctttcttgg ctcatgtggt
       61 tctcaaatgg atacggtaaa aagcccgttc atattaggca atcagcttta aaaccacaag
      121 aacattgact taacttaccg tttccatgct ctaacggttt taagcggcct gtaagcctct
      181 ctagtttgct gaaaatgtga gtcaacacac ctctggtaaa ccccataacc atctgtcgag
      241 tcgatattgc gccaccgcca tgtggccgta gcctgttccc ccctgcccca tcacctcgca
      301 gccaacaagc acatgctcaa agtgcatgtt aaaatccacg cttgccatca gcccacaccg
      361 ccatgaaacc caccatacca ccgagcagtt tgcatcccaa caatccccac caaggccgct
      421 atgatttgga tgccttggcc gtggccgtgc ctgctttaca gtcttttttc atcaccacac
      481 ccagaggcga caccagcata gacttcagca atgccaaagc ggtgatgtgt ttgaatcaag
      541 ccttgctcaa gcatttttac ggcatggaag cctggtcggt gccgcaaggc tttttgtgcc
      601 cacccatacc tgggcgcgcc gattatgtgc atcacgtcgc cgatttgctc gtcaagcggc
      661 ctgtaagccg tcaacaccaa gccgtgcgca tgctcgacat tggcacggga gccaatttga
      721 tataccccat catcgccacc cgcgccttgg gctggcaagt ggtgggcagc gacatcaatg
      781 ccaccgcgct tgcgtatgct caaaaactca tagacgccaa tcctgtctta gcaggcacaa
      841 cgctgcgctt gcaaagcgat gcaggcaagg tgtttacagg cgtaatacaa gccgatgagt
      901 atttcgatgt gacgctgtgc aacccgccat ttttcaaatc ggcagacgag gccaacaaac
      961 aagccgcgcg caaatggcgc aatctcaagg gcgcaaaagc ctcaagcgtg cgcaattttg
     1021 gtggtcaaaa cgcggaattg tggtgtgaag gcggcgaatt ggcgtttttg cgccgcctca
     1081 tcgccgaaag ccacctgttt gcccagcaag tcggttggtt caccagcttg gtttccaacc
     1141 aagaccactt gcccaagcta caacaagcgc tgcgcaaact gcatgcggct gaaatcaaag
     1201 tgatggccat gacactaggc caaaagcgca gccatgtatt ggcatggcgg tttgaataag
     1261 cagcttgtta agtttacagg ccgcatcatt tgaatcgcag cctgtacccc cttgatttgg
     1321 cgcagacatg cccttctgat aggatgcatt cactccaaca caaggcaggt gacatgagcg
     1381 gatttgattt gggcttggtg ctgtatgtgc tatacacagc cctcaccgac tggtgggtgt
     1441 ttcgacacaa tgttgcaccc gataccgatc gcatcagcct gttgcgccaa tgcggcggta
     1501 gcggtgacaa aatggcggcc attattgtct gggcattggt cacgctgtca ttgggatttg
     1561 cagtcatcga agcttggctg tggaccgatt aagcccgttt cagcaaagca atcatcgccc
     1621 ccaccatcgc caagcctatg cccagcacca agttcagact taatggtgtt tcgcccaaga
     1681 gcacactggc cgtgccgcca atcgcgggga aaaaagccac catatagctg cctttgacca
     1741 cccctatgct gcggatcaaa tacacataca tcaacc
//
LOCUS       DAOMVK010000485         1776 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_19470_length_1776_cov_2.679256, whole genome shotgun sequence.
ACCESSION   DAOMVK010000485 DAOMVK010000000
VERSION     DAOMVK010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19470_length_1776_cov_2.679256"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="PK856_11905"
     CDS             <1..895
                     /locus_tag="PK856_11905"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958891.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRL03114.1"
                     /translation="VIIKAAGGGGGRGMRVVEREEDLITSVEMTKTEAGAAFGNPTVY
                     MERYLQKPRHIEIQVLADEHGNAIYLAERDCSMQRRHQKVIEEAPAPGITEEERKTIG
                     EACVAACKRINYRGAGTFEFLYENGEFFFIEMNTRVQVEHPITEIITGVDIVQEQIYV
                     AAGLPLSYTQDDIVIRGHAFECRINAEDPFTFIPSPGLIQTYHAPGGPGIRVDTHIYE
                     GYSIPPYYDSMIGKIISSGHDRNKAMARMRVALSELAVTGIKTNTQLHRELFTDPGFI
                     KGGTSIHYLEQWMEEHHPKGA"
     gene            898..>1776
                     /gene="prmA"
                     /locus_tag="PK856_11910"
     CDS             898..>1776
                     /gene="prmA"
                     /locus_tag="PK856_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRL03115.1"
                     /translation="MPFLQVNIVVPSSHAELLSDELMEVGALSTAIEDAYAGTDKEQP
                     IFDEPNEPSAEIWEQSLIIAMFDADTDVDAAVALLPENMRPVKDAYTVTSVADQDWVK
                     LTQSQFDPIHVSERLWITPTWHEAPTDGSINIELDPGLAFGTGSHPTTFLCLRWLDAN
                     MPQGAAVLDYGCGSGILAITAAKLGAGKVVGVDIDKQAIRASHDNAAQNQVSIEVYLP
                     DAQPEGQFDVVVANILANPLRMLGQMLAGRVKTDGQIVLSGILAEQVEEISALYQQWF
                     DMKPATIQDGWACLFGT"
BASE COUNT          456 a          437 c          464 g          419 t
ORIGIN      
        1 agtcatcatc aaagccgctg gcggtggtgg tggtcgcggt atgcgcgtgg ttgagcgtga
       61 agaagacttg attacgtcag tggaaatgac caaaaccgaa gccggcgctg cgtttggcaa
      121 cccaactgtg tacatggagc gctatttaca aaaaccgcgt cacattgaaa ttcaagtgtt
      181 ggcagacgaa cacggtaatg ccatttattt ggctgaacgc gattgctcta tgcaacgccg
      241 ccaccaaaaa gtgattgaag aagcccctgc accaggcatc accgaagaag aacgcaaaac
      301 cattggtgaa gcgtgtgtgg cggcctgtaa acgcatcaat taccgcggtg cgggcacgtt
      361 cgaattcttg tatgaaaacg gtgaattttt cttcattgaa atgaacacgc gcgtacaagt
      421 agaacacccg attaccgaaa tcatcaccgg cgtagacatt gtgcaagaac aaatctacgt
      481 ggccgctggc ttgcctttga gctataccca agacgacatt gtcattcgtg gccatgcgtt
      541 tgaatgccgc atcaatgcgg aagatccgtt taccttcatt ccaagtcctg gcttgattca
      601 aacctatcat gcgcctggtg gtcctggcat ccgtgtggat acccatattt acgagggtta
      661 ttcgattcca ccgtattacg acagcatgat aggcaaaatc atcagttcgg gccatgatcg
      721 taacaaagcc atggcacgca tgcgtgtggc cttgtctgag ttggcggtga ctggcatcaa
      781 aaccaacacc caattgcacc gcgaattgtt tactgaccca ggtttcatca aaggtggcac
      841 cagcattcac taccttgagc aatggatgga agaacaccat cctaagggag cgtaagcatg
      901 ccgtttttgc aagtaaacat tgtggtgcct tccagccatg cggagttgct gtctgatgaa
      961 ttgatggaag tgggcgctct gtccactgcc atcgaagacg cttacgcggg cacggacaaa
     1021 gaacaaccca tttttgatga gcccaatgaa cccagcgccg aaatttggga acaaagcctc
     1081 atcattgcca tgtttgatgc cgataccgat gttgatgcgg ctgtggcctt gttgcctgaa
     1141 aacatgcggc ctgtaaaaga tgcgtatacc gtgacttcgg tggcagacca agattgggtg
     1201 aaactcaccc aatcacagtt tgacccgata catgtgtctg agcgcttatg gattaccccc
     1261 acttggcacg aagcgccaac cgatggttcg attaacatcg aattagaccc aggtttggcc
     1321 tttggcaccg gcagccaccc caccacattc ttgtgcttgc gttggttaga cgccaacatg
     1381 ccacaaggtg cggcggtgtt ggattacggc tgtggctcgg gcattttggc gattaccgca
     1441 gcaaaattgg gcgcaggcaa agtggtcggt gtggacatcg acaaacaagc catccgcgcc
     1501 agtcacgaca atgccgcaca aaatcaagtg agcattgagg tgtatttgcc agatgcacag
     1561 ccagaaggcc aatttgatgt ggtggtggcg aatattttgg ccaacccact gcgcatgttg
     1621 ggtcaaatgt tggccggtcg cgtcaaaaca gacggtcaaa tcgtcttgtc gggcattttg
     1681 gccgaacaag ttgaggaaat ctctgcgcta tatcaacaat ggtttgatat gaagcctgct
     1741 acaatccaag acggctgggc ctgcttattt ggtact
//
LOCUS       DAOMVK010000486         1774 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_19499_length_1774_cov_2.923211, whole genome shotgun sequence.
ACCESSION   DAOMVK010000486 DAOMVK010000000
VERSION     DAOMVK010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19499_length_1774_cov_2.923211"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PK856_11915"
     CDS             complement(<1..198)
                     /locus_tag="PK856_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_074979792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HRL03116.1"
                     /translation="MVMFQKILIANRGEIACRVIRTAKKLGVKTVAVYSDADANAQHV
                     LLADEAVYIGAAPARESYLQAD"
     gene            complement(209..1009)
                     /locus_tag="PK856_11920"
     CDS             complement(209..1009)
                     /locus_tag="PK856_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRL03117.1"
                     /translation="MSETYISLEQQGAVATVWLERPEVHNAFNADLIRQLKDCFVALN
                     QRDDVRVVVLAGRGKSFSAGADLNWMKAAGAASEAENQADAQNLADMLYALATLKQPT
                     IARVQGAAMGGGMGLAATCDICIAGDKAMFATSEVRLGLAPSTISPYVIRAIGERQAQ
                     RYFLTAERISAAKAAEIGLAHEVVGMEALDAKVEEIVAALLLGGPVAQQASKQLIALV
                     AKRDNSAELRTQTAAHIASVRAGQEAKEGLNAFLEKRAAAWCNSANPT"
     gene            complement(1035..>1774)
                     /locus_tag="PK856_11925"
     CDS             complement(1035..>1774)
                     /locus_tag="PK856_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRL03118.1"
                     /translation="GIVPTDTRKPYDVREIIARIVDGSKFDEFKARFGTTLICGFARL
                     YGMPVGIIANNGILFSESAQKGAHFIELCCQRNIPILFLQNITGFMVGRKYENEGIAR
                     NGAKLVMAVATAKVPKITLVIGGSFGAGNYGMCGRAYSPRFLWTWPNSRISVMGGEQA
                     ASVLSTLKRDGIEAKGGEWSAAEEDAFKQPIREQYERQGHPYYASARLWDDGVIDPAQ
                     SRRVLGLSLSAALNAPTEATQFGVFRM"
BASE COUNT          397 a          519 c          446 g          412 t
ORIGIN      
        1 atcggcctgt aaatagcttt cacgcgcggg tgccgcgccg atgtacacgg cttcatcggc
       61 cagcaatacg tgttgcgcat tggcatcggc atcggagtac acggccacgg ttttcacgcc
      121 cagtttttta gcggtacgaa tcacgcggca agcaatctca ccacgattgg caatcaaaat
      181 cttttgaaac ataacaattc cttcgtggtt aagttgggtt agcgctgttg caccaagcgg
      241 ctgcgcgttt ttccaaaaac gcattcaaac cttctttggc ctcttgccct gcacgcacac
      301 tggcaatgtg ggcagcggtt tgggtgcgca gctcagcgct gttgtcgcgc ttggccacca
      361 atgcaatcaa ttgcttggaa gcctgttgcg ccacagggcc acccaataac aaagcggcca
      421 cgatttcttc tactttcgca tccaatgcct ccatgcccac cacttcgtgc gccaaaccga
      481 tttcggcggc tttggcagcc gaaatgcgtt cagcagtgag gaagtagcgc tgtgcttggc
      541 gttcaccgat ggcgcgtatc acatacgggc tgatggtgga tggtgccaag cccaagcgca
      601 cttctgaagt ggcaaacatg gctttgtcgc cagcgatgca aatgtcacaa gtggccgcca
      661 agcccatgcc accacccatg gccgcgcctt gtacgcgggc aatcgtgggc tgtttcaaag
      721 tggccaaggc ataaagcatg tcggccaagt tttgtgcatc ggcttggttt tcagcttcgg
      781 aagccgcgcc tgctgccttc atccaattca aatcggcacc tgccgaaaaa ctcttaccac
      841 gccctgccaa caccaccacg cgcacatcgt cgcgctgatt caaagccaca aaacagtctt
      901 tcaattggcg tatcaagtct gcattaaacg cattgtgcac ttcaggccgc tccagccaca
      961 ccgtggccac tgcgccttgc tgctccaaac tgatgtaagt ttcgctcatg tcatttttcc
     1021 ttattattgt tgttttacat gcggaacacg ccaaattgcg tggcttcggt tggggcattc
     1081 aaggcagcag acaggctcaa gcccaacaca cggcggcttt gcgcagggtc gatgacgcca
     1141 tcgtcccaca agcgtgccga cgcataataa ggatggcctt ggcgctcgta ttgctcgcgt
     1201 atcggctgct tgaacgcatc ttcttctgct gcgctccact caccgccttt ggcttcaatg
     1261 ccatcgcgtt tcaaggtaga caaaacgctc gccgcttgct cgccgcccat caccgaaatg
     1321 cgtgagttag gccacgtcca caagaagcgt ggcgagtaag cgcggccaca catgccgtaa
     1381 ttgcctgcgc caaacgagcc gcctatcacc aaggtgattt tcggcacttt ggcagtggcc
     1441 accgccatca ccaatttcgc gccattgcgg gcgatgcctt cgttttcgta tttgcgaccc
     1501 accataaagc cggtgatgtt ttgcaagaac aaaatcggga tattgcgctg gcaacacaat
     1561 tcaatgaaat gcgcgccttt ttgtgccgat tctgaaaaca aaatgccgtt gttggcgatg
     1621 atgcccacgg gcatgccata caagcgcgca aagccacaaa tcaaagtggt accaaagcgg
     1681 gctttgaatt catcaaattt cgagccatcg acaatgcgag caatgatttc gcgcacatcg
     1741 taaggcttgc gggtatcggt aggcacgatg ccgt
//
LOCUS       DAOMVK010000487         1771 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_19543_length_1771_cov_2.259324, whole genome shotgun sequence.
ACCESSION   DAOMVK010000487 DAOMVK010000000
VERSION     DAOMVK010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19543_length_1771_cov_2.259324"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..407)
                     /locus_tag="PK856_11930"
     CDS             complement(153..407)
                     /locus_tag="PK856_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HRL03119.1"
                     /translation="MSLFITDECINCDVCEPECPNDAIYQGDEIYEINPSLCTQCVGH
                     YDDPQCQQVCPVDCILIDEVNPETEEQLQDKYEKLQADKN"
     gene            complement(492..1055)
                     /gene="rsmD"
                     /locus_tag="PK856_11935"
     CDS             complement(492..1055)
                     /gene="rsmD"
                     /locus_tag="PK856_11935"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HRL03120.1"
                     /translation="MKNKQHTQQVRIIGGTHRRRIVKFDEAEGLRPTPDRVREQVFNW
                     LGQRLNGLKVLDLFGGSGVMAFESASRGASQVDVVELNRQTVQNMRAVIKELQLECVN
                     VHQQDAKMYLQAASVVYDVIVLDPPYRWQDWDALWGLLQTRCHNDTVLYIEAAQLPQL
                     PEWLQWTKQDKAGQSHYGLALVQDIDL"
     gene            complement(1095..1583)
                     /locus_tag="PK856_11940"
     CDS             complement(1095..1583)
                     /locus_tag="PK856_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRL03121.1"
                     /translation="MQTRWWLCRRCEQADDLVAVINYLLAELIENRQIPVVKLTVQAQ
                     NALRQYDWPGNLEQLKSVLKSLALNADHGTVDAPQVVALLSQFKQPQTNHVSGGFNFD
                     LPLRELREELEKRYFEYHITQENGNMSRVAQRVGLERTHLYRKLKQLGVQFSKRQVEK
                     AD"
     gene            complement(1537..>1771)
                     /locus_tag="PK856_11945"
     CDS             complement(1537..>1771)
                     /locus_tag="PK856_11945"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HRL03122.1"
                     /translation="QKAQQGVLYLGDVSQLSIAAQRAVVELIQKADQYPARIVAYCSR
                     PLEEMLTQSEYESTLINAFANSMVAVPPLRASR"
BASE COUNT          470 a          471 c          336 g          494 t
ORIGIN      
        1 gctttcgcaa gagatttaca gtctcccccc tttggccgct cgggaatccc tccacaagag
       61 aggctgcata ttaatgcacg acctcttggc agtcaacctt aattttcagt gtatttccgt
      121 aaaataaatt aacaatctgt tttttataaa aattaatttt tgtctgcttg cagtttttca
      181 tacttatcct gcaactgctc ttcagtctca gggtttactt catcaatcaa aatacagtct
      241 actgggcaga cttgttggca ctgcgggtca tcatagtgac ccacacactg ggtacacaga
      301 ctcgggttga tttcataaat ctcatcaccc tggtatatgg cgtcatttgg gcattctggc
      361 tcacacacgt cgcagttaat gcactcatcg gtaataaaca aagacatttg ctgtttccat
      421 caattaagca taacaaacct gatattgtta cagtttcagg acttattggc aaattatttg
      481 ccgaagcaca attataaatc aatatcttgc actaatgcca aaccataatg actttgaccc
      541 gctttatctt gcttagtcca ttgtaaccat tcaggtagtt gtggcaattg cgctgcctca
      601 atatacaata cggtatcatt gtgacaacgt gtttgcagca agccccacag cgcatcccaa
      661 tcctgccaac ggtaaggcgg atccaataca atgacatcgt aaacaacaga agcggcctgt
      721 aaatacattt tggcatcttg ctgatgcaca ttgacacact ctagctgcaa ttctttaatc
      781 acagcgcgca tgttttgtac ggtttgccga ttcaattcca ccacatccac ttgactggca
      841 ccacgtgatg cagattcaaa cgccatcaca ccactgccac caaacaaatc caataccttc
      901 aaaccattca ggcgctgccc cagccagtta aatacctgct ctctgacccg atcaggcgtg
      961 ggtctaagac cttcagcctc gtcaaatttc acgatgcgac gacgatgggt accaccaata
     1021 atgcgaactt gctgtgtgtg ttgtttgttt ttcatatgtc ttaaactaaa aagctgcctt
     1081 tcggcagctt gtcatcaatc ggctttttca acttgtcgct tggagaactg cacacccagt
     1141 tgtttcaact tacggtacag atgcgtccgc tccaaaccca ctctttgcgc aacacggctc
     1201 atgttgccgt tttcttgcgt aatgtggtat tcaaaataac gcttttccag ctcttcacgc
     1261 aactcacgca aaggcaagtc aaaattaaac ccacctgaaa catggttggt ttgcggctgc
     1321 ttaaattgcg acaacaaagc caccacttgc ggcgcatcca ccgtgccatg gtctgcattc
     1381 aaagccaagc ttttcaggac gctttttaac tgctccaaat taccaggcca atcgtattgg
     1441 cgcaaagcat tttgcgcttg cactgtcagc ttcaccaccg gaatttgtcg gttttcaatc
     1501 aattcagcca gtaaataatt aatcaccgcc accaaatcat cggcttgctc gcagcggcgg
     1561 cacagccacc atcgagtttg caaaagcatt gatgagtgtc gattcatatt cagattgcgt
     1621 cagcatctct tccaaagggc gactgcaata agccacaatg cgggctggat actggtcggc
     1681 tttttgaatg agctccacca ctgcacgctg cgcggcaata gacagctgtg acacatcacc
     1741 caaatacagc acaccttgct gtgctttttg c
//
LOCUS       DAOMVK010000488         1755 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_19778_length_1755_cov_2.502941, whole genome shotgun sequence.
ACCESSION   DAOMVK010000488 DAOMVK010000000
VERSION     DAOMVK010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19778_length_1755_cov_2.502941"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /gene="fumC"
                     /locus_tag="PK856_11950"
     CDS             <1..187
                     /gene="fumC"
                     /locus_tag="PK856_11950"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957558.1"
                     /note="class II family (does not require metal);
                     tetrameric enzyme; fumarase C; reversibly converts
                     (S)-malate to fumarate and water; functions in the TCA
                     cycle; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HRL03123.1"
                     /translation="ENSLMLVTALAPHIGYDAAAKIAKTAHHQGTTLREEALASGLVS
                     DEEYDAWVRPEDMISPH"
     gene            277..606
                     /locus_tag="PK856_11955"
     CDS             277..606
                     /locus_tag="PK856_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HRL03124.1"
                     /translation="MEICIFCRIVAGELPADVVYEDDAIMAFKDTHPEAPVHLLVIPK
                     KHIASLAHVQTEDAGLMAQLMMALPELAVQQGMEQGFKTRINTGRAGGQEVAHLHVHL
                     TGRPSLK"
     gene            664..873
                     /gene="tatA"
                     /locus_tag="PK856_11960"
     CDS             664..873
                     /gene="tatA"
                     /locus_tag="PK856_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HRL03125.1"
                     /translation="MGGLSIWHWVLVLVIVVLVFGTKKLRNAGRDIGGAVHDFKKGMN
                     EGNAEIEAGKKTKEADAIEHEKKDV"
     gene            877..1410
                     /gene="tatB"
                     /locus_tag="PK856_11965"
     CDS             877..1410
                     /gene="tatB"
                     /locus_tag="PK856_11965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HRL03126.1"
                     /translation="MFELSFGEILLFTIVALIVLGPERLPALARFLGRWMAKIQNLVS
                     NVKAEISAEIAVDDLKQMRDEFQASAQTIRDDLRNQAAQLQQVKQEVGDIPYWDRLPP
                     QKRPEDFGIISEDSDMAEERAWLLAQQQAGEHDTRGRSLSQLSRRRRKDIRPYQHINR
                     QAQRIGARRQMKQSRHG"
     gene            1403..>1755
                     /locus_tag="PK856_11970"
     CDS             1403..>1755
                     /locus_tag="PK856_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRL03127.1"
                     /translation="MDDTQPLIEHLIELRSRLIRTLIGLLLCFVALVPFSEQLYTFVA
                     QPLMSSLPAGNQMIATDVIAPFFVPMKVTLMLAFLASLPNTLWQVWAFVAPALYQNEK
                     KLVLPLVVSTVLLFFA"
BASE COUNT          457 a          373 c          473 g          452 t
ORIGIN      
        1 ggaaaattct ttgatgctgg tcaccgcttt ggcgccgcac attggttatg atgcggcagc
       61 gaaaattgcc aaaaccgctc accatcaagg cacaaccttg cgtgaagaag ccttggccag
      121 tggtttggtc agtgatgaag aatacgatgc atgggtgcgg ccggaagaca tgatttcgcc
      181 gcattgagtg tgttgttcat ggacttgatg gtgttttgtg tttaggtgga tgggcaaaac
      241 acgcttataa taccgacatc agcacaggag taacgtatgg aaatatgtat tttttgccgc
      301 atcgtggcag gtgagttgcc tgcggatgtg gtgtatgaag acgatgccat catggccttc
      361 aaagacaccc atccagaagc acctgtgcac ctgttggtca tccctaagaa gcacattgcc
      421 tctttagcgc atgtgcaaac cgaagatgct gggctcatgg cgcaattgat gatggcattg
      481 cccgaacttg ctgtgcaaca aggcatggag cagggattta aaacgcgaat taatacggga
      541 cgagccggtg gacaagaggt ggcacattta catgtacacc ttacaggccg ccccagtttg
      601 aaataaaggc atgcagtcat gactgcagcc tgtaaatgaa acacatagag taaaggaagc
      661 atcatgggtg gtttgtctat ttggcattgg gttttggttt tggttatcgt ggtattggtt
      721 tttggtacca aaaaattgcg caacgcaggc cgcgacatcg gcggtgcggt acatgacttt
      781 aaaaaaggca tgaacgaagg caatgctgaa attgaagctg gcaaaaaaac caaagaagcc
      841 gatgcgattg aacacgagaa aaaagacgtg taatcaatgt ttgaattgag ttttggtgag
      901 atattgctgt tcaccattgt ggcattgatt gttttggggc cggagcgttt accggcctta
      961 gcgcgttttt tggggcgttg gatggccaaa attcaaaact tggtcagcaa tgttaaagcc
     1021 gaaatcagtg cggaaattgc cgtcgatgat ttaaagcaaa tgcgtgatga atttcaagct
     1081 tcggcacaaa ccatacgcga tgatttgcgc aatcaagcgg cacaattaca gcaagtgaag
     1141 caagaggtag gtgacatccc ttattgggat agattgccac cacaaaagcg gcctgaagat
     1201 tttggcatca tcagtgaaga ctcagacatg gcagaagagc gcgcatggtt gctggcgcag
     1261 caacaggctg gcgaacatga cacacgtggc cgttctttgt ctcaattaag tcgccgccgc
     1321 cgcaaagaca tacgcccata ccaacacatc aatcggcagg cgcagcgcat tggtgcgcgc
     1381 cgtcaaatga aacagagccg tcatggatga tacccaacct ttaattgagc atttgattga
     1441 gctcagaagc cgcttaatcc gcaccttaat cggtctgcta ttgtgttttg tggccttggt
     1501 gccgttttct gagcaattgt atacgtttgt cgcccagcct ttgatgagca gtttgcctgc
     1561 aggcaatcaa atgatagcca ccgacgtgat cgccccattt tttgtgccga tgaaagtgac
     1621 attgatgctg gctttcttgg ccagtttgcc caataccttg tggcaagtgt gggcatttgt
     1681 cgcgcctgct ttgtaccaaa atgaaaaaaa actggtcttg cccttggtgg tgtcgacggt
     1741 gttgttgttt tttgc
//
LOCUS       DAOMVK010000489         1755 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_19781_length_1755_cov_1.967647, whole genome shotgun sequence.
ACCESSION   DAOMVK010000489 DAOMVK010000000
VERSION     DAOMVK010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19781_length_1755_cov_1.967647"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="PK856_11975"
     CDS             complement(<1..825)
                     /locus_tag="PK856_11975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRL03128.1"
                     /translation="MLPVSVLDLSPINVGASIAESFAHSVELAQTAERLGYKRIWFAE
                     HHNMPGIASAATSVLLCHIGNHTQSIRIGAGGIMLPNHSPLAIAEQFGTLATLFGDRV
                     DLGLGRAPGSDPATMRAMRRHGHQEEDSFPDDVVDILHYFDDAGSSRIQAIPGMGTHI
                     PVWILGSSTYGAQLAAHLGLPYSFASHFAPQLLGQAIHLYRSNFKPSKHLSKPYVTLA
                     VNVVMADTVEEAQYHYTSLQQSFLSNRRGQRSAVPRPVENMDDIWNEAEKAMVMQGL"
     gene            complement(921..>1755)
                     /locus_tag="PK856_11980"
     CDS             complement(921..>1755)
                     /locus_tag="PK856_11980"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HRL03129.1"
                     /translation="AGRELIRRLQEQFQADGLKVSLSRLCTWFGVARRSVYYRSHKKA
                     AVVKACYAEPIKQLIEAEPSFGYRTVAALLGMNKNTVQRVFQLKGWQVRKRSVGRRPR
                     IQAVPSKAQEPNQRWSTDLCRVWGGQDGWLSLALVMDCHTRELLGWQLSKTGKASTAV
                     SALEHALIYRFLCLDKVPERFLLRSDNGLVFTSRKYTALVKSYGLQQEFITPHCPQQN
                     GMVERLIRTIKEQCVHRHRFESLQHANRVLSDWIQFYNEVRPHQALGMKTPAKAYALS
                     L"
BASE COUNT          400 a          449 c          486 g          420 t
ORIGIN      
        1 caacccctgc atcaccatgg ctttttcggc ttcgttccaa atgtcatcca tgttttccac
       61 tgggcgaggc acggctgagc gttggccacg gcggttgctg aggaaagact gttgcaatga
      121 ggtgtagtgg tattgcgctt cttctaccgt gtctgccatg accacattga cggccaaagt
      181 cacataaggc ttggacaggt gtttggatgg tttgaaattg ctgcggtaca aatggatggc
      241 ttggccgagc aattgcgggg cgaagtgtga ggcaaacgag taaggcaggc ccaaatgtgc
      301 ggccagttgc gcgccgtaag tgctggagcc gaggatccac acggggatgt gtgtgcccat
      361 gccggggatg gcttgtatgc gcgagctgcc ggcatcgtca aaataatgca agatgtcgac
      421 cacgtcatcg gggaagctgt cttcttcttg atggccgtgg cggcgcatgg cgcgcatggt
      481 ggcagggtcg ctgcctgggg cgcggcctag gcccaagtcc acgcggtcgc caaacaaggt
      541 ggccaaggtg ccaaattgct ctgcaatcgc caatgggctg tggttgggca gcatgatgcc
      601 gcctgcgcct atgcgtatgc tttgggtgtg gttgccgatg tggcacaaca agactgaggt
      661 ggcggcagag gcgatgccgg gcatgttgtg atgctcggca aaccaaatgc gtttatagcc
      721 taagcgttcg gctgtttggg ccagttcgac cgagtgggca aatgattcgg cgatgctggc
      781 gccgacattg atgggactta aatccagaac cgaaaccggc aacatattcc gctccctaaa
      841 tgacgtttac attgagtata gcgggttttg ttggccggtg tcagcagtga aatgtaatga
      901 cctagcactt tctgcacaag tcacaagctt aatgcatagg ccttagcagg ggttttcatg
      961 cccagtgctt ggtgaggcct gacttcgttg taaaactgta tccaatcact taacacccga
     1021 ttggcatgct gtaaactttc aaaacggtgg cggtgtacac actgttcttt gatggttcgg
     1081 atcaagcgct ccaccatccc attttgctgg gggcaatgcg gggtgatgaa ttcctgctgc
     1141 aagccatagc ttttaaccaa cgcagtgtat tttcgactcg taaacaccag gccattgtcc
     1201 gagcgcagta agaaccgctc aggcactttg tctaagcaga gaaagcggta aatcagagca
     1261 tgttccagtg cactgacggc agtgctggct ttacctgttt tggaaagttg ccaacccaat
     1321 aattcacggg tatggcaatc cattaccaaa gccaagctta gccaaccatc ttgaccaccc
     1381 caaacccgac acaaatcagt agaccaacgc tgattcggtt cttgggcttt agatggcact
     1441 gcttgaatgc gcggtctgcg gcccactgaa cgcttgcgaa cctgccaacc tttgagctga
     1501 aacacacgtt gtaccgtgtt tttgttcatg cccaataagg ctgcaaccgt tcgatagccg
     1561 aatgatggct cagcctcaat caattgcttg atgggctcgg cataacaggc tttcaccacc
     1621 gctgcttttt tgtgtgaccg ataataaact gaacgacgag ccacaccaaa ccaagtacac
     1681 aaacggctca ggctcacttt gagcccatct gcctgaaact gctcctgcaa acgtcgaatc
     1741 agttctcgtc ccgct
//
LOCUS       DAOMVK010000490         1753 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_19808_length_1753_cov_2.642521, whole genome shotgun sequence.
ACCESSION   DAOMVK010000490 DAOMVK010000000
VERSION     DAOMVK010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19808_length_1753_cov_2.642521"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..673)
                     /gene="ispZ"
                     /locus_tag="PK856_11985"
     CDS             complement(92..673)
                     /gene="ispZ"
                     /locus_tag="PK856_11985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein IspZ"
                     /protein_id="HRL03130.1"
                     /translation="MNAKALDAVKPFLDWIPLIVFFYIYKTTDHTGNQHIIAATTGLM
                     ITTLIVYGLMFVLQKFSLEKRQWLVVVLTVVFGGLTMAFQDDFYIRLKAPIINAVFAF
                     GMAMSPLFLGGQPGIQKMLGQVFEMTDKQWMKLNWVWVAFFCFMAFLQAMFAFVWKEY
                     WAEFTAFGDIIVMVVFMISQFWFLRRYMRQDVK"
     gene            960..1418
                     /locus_tag="PK856_11990"
     CDS             960..1418
                     /locus_tag="PK856_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03131.1"
                     /translation="MKKWLMAACFWGCVSSALASLSMPTVEGLALGETLNATKKKLGK
                     PLRQNYSTPNVCNNESLDLQLHYKGMVVTLIESARHQYIVSDVVITGKPWKLNGVGIG
                     DNSTTVERKFGRTKTNNGVMPYNKRGEPYGFDFIMHKDKVSKISYNSYLC"
     gene            complement(1517..>1753)
                     /locus_tag="PK856_11995"
     CDS             complement(1517..>1753)
                     /locus_tag="PK856_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HRL03132.1"
                     /translation="IDAQAWELPKLFQWLQKAGNVEAQEMYRTFNCGIGMVVIVAAED
                     ADQAQAFLAEQGETVYRLGAIRERQGDEHQTQVA"
BASE COUNT          502 a          438 c          367 g          446 t
ORIGIN      
        1 cacttaacca taaataagcg gcctgtaatg gtttacaggc cgcttatttg ttctcacatg
       61 ctgatttcaa gcatgtttat ttcatgagtg cttatttgac gtcttggcgc atgtagcggc
      121 gcaaaaacca aaattggctg atcatgaaca ccaccatcac aatgatgtcg ccaaacgccg
      181 tgaactcagc ccaatactct ttccacacaa aggcaaacat cgcttgtaag aaggccataa
      241 agcagaagaa cgccacccac acccaattga gtttcatcca ctgcttgtct gtcatctcaa
      301 acacttgacc aagcattttt tggatgccag gctgtccacc caaaaacaag gggctcatgg
      361 ccatgccaaa cgcaaacacc gcattgatga tgggcgcttt caagcggatg taaaaatcgt
      421 cttggaaggc catggtcaag ccaccgaaca ccaccgtcaa caccaccacc aaccattggc
      481 gtttttccaa actgaatttt tgcaacacaa acatcaagcc gtacacaatc agcgtggtaa
      541 tcatcaagcc tgtggtggcc gcaatgatgt gttgattgcc cgtgtggtca gtggttttgt
      601 agatatagaa aaaaacgatt aacggtatcc agtctaaaaa cggctttacc gcatccaagg
      661 cttttgcgtt catgcttgcg tatcaataaa aattaaacag gccgtattgt agccttaatt
      721 taagccactg ccgtcatccg tctgcgttaa ttgccgcaaa aatcacaatt gtgcaagtgc
      781 tcgagcatgg gtacactgtc gacaattttg tgaacgcgac acataatttt aacctttttg
      841 acacatactg atttaatatc accccatctg tccctttaac aaaacacctt tatatcagtg
      901 ttttggagac agaaatgtag tgatacctgt gtgttagcaa tgcttgaaga aagaatggta
      961 tgaaaaaatg gctgatggca gcatgttttt ggggctgtgt cagtagtgca ctggcgagcc
     1021 tcagcatgcc cacggtagag ggcttggcct tgggcgaaac cctcaacgca accaaaaaga
     1081 aattgggcaa acctttgcgc caaaactact ccacccccaa tgtgtgcaac aacgaatcac
     1141 tcgatttgca attgcactac aaaggcatgg tggtgacttt gattgaatcc gcacgccacc
     1201 aatacatcgt cagcgatgtg gtcatcacag gcaaaccatg gaaactcaat ggtgttggca
     1261 tcggcgacaa cagcaccact gtcgagcgca agtttggtcg caccaaaacc aacaatggcg
     1321 tcatgcctta caataaacgt ggcgaacctt atggctttga cttcatcatg cacaaagaca
     1381 aagtcagcaa aatcagctac aactcttatt tgtgctgaat ccagcaacgc aaaagtggcc
     1441 tgtacattta caggccactt tttaatgctt aaaaccgatt aaacgaatga attaaccgat
     1501 ttgattcagt cgcatattag gcgacttggg tttggtgctc atcgccttga cgctcacgaa
     1561 ttgcgcccaa acggtaaacg gtttcacctt gctcagccaa gaatgcttgg gcttggtcag
     1621 cgtcttcagc cgccacgatt accaccatgc ctatgccgca gttgaatgtg cggtacattt
     1681 cttgggcttc cacattgcct gctttttgca accattggaa caattttggc aattcccaag
     1741 cttgggcatc gat
//
LOCUS       DAOMVK010000491         1748 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_19888_length_1748_cov_2.503839, whole genome shotgun sequence.
ACCESSION   DAOMVK010000491 DAOMVK010000000
VERSION     DAOMVK010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19888_length_1748_cov_2.503839"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..1446
                     /locus_tag="PK856_12000"
     CDS             61..1446
                     /locus_tag="PK856_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HRL03133.1"
                     /translation="MSESIAAEIFKAYDIRGIVDVTLTEEAVYHIGLAIAARARDLGV
                     TDIAIGRDGRLSGPTLLARLADALQDGGLNVTDVGMVATPMLYYAAHTLTQGSGVMLT
                     GSHNPPQYNGLKMMLAGTTLSGDSIQDLYQAIVAGQATVAAQRGSFSRTDVANEYRER
                     IQRDIQLARPMNIVIDAGNGVAGAYAGDVYRALGCTVTELFCEVDGTFPNHHPDPAKP
                     ANLQDLIHALQTSDAEIGLAFDGDGDRLGVVTKDGNIIWPDRQLMLYAADVLKRQPQA
                     KIIYDVKCTRLLAPWIREHGGEPIISRTGHSLIKAAMKEHQAALAGEMSGHTFFKERW
                     FGFDDGMYTGARLLEILSQSADASAVLNALPEALSTPELNISMAGEGDNHRIITTLQQ
                     NAKFPLAQNIIDIDGLRVEYADGFGLIRASNTTPVLVLRFEAETEAALAAIQAEFKQV
                     LQQVGLNDLPF"
     gene            1512..>1748
                     /locus_tag="PK856_12005"
     CDS             1512..>1748
                     /locus_tag="PK856_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03134.1"
                     /translation="MGRVQQTHRLNKAFITITNSEIETLENQKRPVNPSKGLQAAFIS
                     ILKLLFSVLVLPNRGCRASLHWFSPRWRSSLVRFP"
BASE COUNT          436 a          502 c          428 g          382 t
ORIGIN      
        1 gccagccgcc aacaattgat agcattaacc caagcattaa cccaagcgta aggaccgtcc
       61 atgtcagaga gtattgcagc agaaattttc aaagcctatg acatacgcgg cattgttgat
      121 gtcaccttaa ccgaagaggc cgtgtaccac atcggtttgg ccatcgccgc gcgcgcgcgc
      181 gacttaggtg tcactgatat tgccatcggt cgcgacggcc gtttgtctgg cccaactttg
      241 ttggcacgcc tggccgatgc cttgcaagat ggcggcctga atgtcaccga tgtgggcatg
      301 gtcgccacac cgatgttgta ttacgccgcc cacaccttaa cccaaggctc aggcgtgatg
      361 ctcacgggca gccataaccc cccccaatac aacggtttga aaatgatgtt ggcaggcacc
      421 actttgtctg gcgacagcat ccaagatttg taccaagcca ttgtggctgg ccaagccacg
      481 gtcgctgccc aacgtggtag ctttagccgc accgatgtcg ccaatgaata ccgcgaacgt
      541 atccaacgcg acatccaact cgcacgcccc atgaacatcg tcattgatgc aggcaatggc
      601 gtggcaggtg cttacgcggg cgatgtttac cgtgccttag gctgcaccgt caccgaattg
      661 ttttgcgaag tggacggcac cttccccaac caccatccag acccagccaa acccgccaat
      721 ttgcaagact tgatacacgc cttgcaaacc agcgatgccg aaattggctt ggcctttgat
      781 ggcgatggcg accgtttggg cgtggtcacc aaagatggca acatcatttg gccagaccgc
      841 caattgatgc tgtacgcagc cgatgttctc aagcgccagc cacaagccaa aatcatttac
      901 gacgtgaaat gcacgcgctt attggcgccg tggatacgcg aacacggtgg cgagcccatc
      961 atcagccgca caggccacag cttgattaaa gccgcgatga aagaacacca agccgctttg
     1021 gcaggcgaaa tgagcggaca caccttcttc aaagagcgtt ggtttggctt tgacgatggc
     1081 atgtacacag gcgcacgcct gttggaaatt ttgtcgcaat ccgccgatgc cagcgctgtg
     1141 ctcaacgcct tgcctgaagc cttgtccaca cccgagctga acatcagcat ggcaggcgaa
     1201 ggcgacaatc accgcatcat caccaccttg caacaaaatg ccaaattccc attggcacaa
     1261 aacatcatcg acatcgatgg tttgcgcgtg gaatacgccg atggctttgg cttaatccgc
     1321 gcgtccaaca ccacacccgt gttggtattg cgttttgaag ccgaaaccga agccgctttg
     1381 gctgccattc aagccgagtt caaacaggta ttgcaacaag tgggcttgaa cgatttgccg
     1441 ttctaaagct taaatcgtgt acaaacaagc ggcctgtaag ggatttatcc ctcttacagg
     1501 ccgcttaatg tatgggcaga gtgcagcaaa cacaccgatt aaacaaggct ttcatcacca
     1561 tcacaaacag tgaaatagaa acccttgaaa accaaaagcg gcctgtaaac ccctcaaaag
     1621 gtttacaggc cgcttttatc tcaatactca agctattgtt tagcgttttg gtgctgccaa
     1681 atcgcggatg tcgcgccagc cttcattggt tttcaccaag gtggcggtca agtcttgttc
     1741 gttttcca
//
LOCUS       DAOMVK010000492         1745 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_19934_length_1745_cov_4.034320, whole genome shotgun sequence.
ACCESSION   DAOMVK010000492 DAOMVK010000000
VERSION     DAOMVK010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19934_length_1745_cov_4.034320"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PK856_12010"
     CDS             <1..224
                     /locus_tag="PK856_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03135.1"
                     /translation="AQALQVNVLWFMAIHNVCAAFLLMSSPGKIEMALSLSGLNDAAS
                     RRWLTRRMLAVAAAVVGILTMVNVLLALS"
     gene            complement(382..975)
                     /locus_tag="PK856_12015"
     CDS             complement(382..975)
                     /locus_tag="PK856_12015"
                     /inference="COORDINATES: protein motif:HMM:NF025501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4303 domain-containing protein"
                     /protein_id="HRL03136.1"
                     /translation="MNPSPFHPELDTALRTYLQAAFDWLRSHHQDEHLYAVGLGMVED
                     ICGFFIVGNTLERLADLHAETAKESAEDQIYYDWSISEWYGNEFEGLPNLVHNAATAI
                     SDDLDDDAYDAFRVTYQDHILHVLADMRGQGLLQNAQGEELWVWVQYADAYDEDFDDV
                     SFARLNSPELSKLFALRYQPKKHSLNHVLRERLQALR"
     gene            complement(1062..>1745)
                     /locus_tag="PK856_12020"
     CDS             complement(1062..>1745)
                     /locus_tag="PK856_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRL03137.1"
                     /translation="KQAADVEDLIQQGVKLIIINPVDSAAVASAIESANKAGIPVITV
                     DRSAGGGKVISHIASDNVAGGKLAGEYMLKSIAAGSDVAMLEGVAGSSAAIERGEGFT
                     KAVEGKVKIVASQTADFDRSKGLSVMENILQANPNIKAVFAQNDEMALGALEAIAAAK
                     KDIMVIGFDATSDAVAKVKEGKLAATVEQQPKLIGEQAIDAAVKYLSGEQVPASIPVE
                     LKLVTKDAQ"
BASE COUNT          422 a          422 c          434 g          467 t
ORIGIN      
        1 ttgcccaagc cttacaggtg aatgtgttgt ggtttatggc catacacaat gtgtgcgctg
       61 cctttttgtt gatgtcgtca ccagggaaaa ttgaaatggc tttgagttta agcggcctga
      121 atgatgccgc aagccgccgt tggttaacac gcagaatgtt ggctgtggcc gctgctgtgg
      181 tggggatatt aaccatggtg aatgtgttgc tggccttgtc atgattacca cttgtgtttg
      241 ggttagccat caaaccatac acagcaaata aatcaataca gacccgtgca agcttcaaga
      301 agtaatggca gggtttcaat caacaataat aagcggcctg taaatctttt acaggccgct
      361 tatgcatggt ttcatccatt cttaccgtaa tgcttgtaag cgttcacgca gcacatggtt
      421 gaggctgtgt tttttgggct gatagcgcaa agcgaacagc ttgctgagtt caggcgaatt
      481 gaggcgggca aagctgacat cgtcaaaatc ttcatcatag gcatcggcat attgcaccca
      541 cacccacaat tcctcacctt gggcattttg cagcaagcct tggccgcgca tgtcggcaag
      601 cacatgcaag atatggtctt ggtaagtcac gcggaaagca tcgtaagcat catcatccaa
      661 atcgtcagaa atggcggtgg cggcgttgtg caccaaattg ggcaagcctt caaattcatt
      721 gccataccat tccgaaatgc tccaatcgta atagatttgg tcttctgctg actccttggc
      781 agtttcagca tgcaaatccg ccaaacgctc caatgtgttg ccgacgatga aaaagccaca
      841 aatgtcttcc accatgccca aacccacggc atacaaatgc tcatcttgat ggtggctgcg
      901 caaccaatca aaagcggcct gtaaataggt gcgcaaggca gtatcgagtt cggggtggaa
      961 tggggatggg ttcatttggc attccttgtg cgtgatgaat aagcaataaa aaagcgtcct
     1021 gtaatcttac aggacgcttg ctctaaaaat aaagtattta cctattgggc atctttggta
     1081 accaatttca attccactgg aatcgaagca ggcacttgtt cgccgctcag gtatttcacg
     1141 gcggcatcga tggcttgttc acctattaat tttggctgtt gttcaacggt agcggccaat
     1201 ttaccttctt tgactttggc cacggcatcg gaagtggcgt caaaaccaat caccatgatg
     1261 tcttttttgg ccgcggcgat ggcttccaat gcacccaagg ccatttcgtc gttttgggcg
     1321 aacacggctt tgatgttagg gttggcttgc aagatgtttt ccatcacaga caagccttta
     1381 gagcggtcga aatcagcggt ttggctggcg acgattttca ctttgccttc tacggctttg
     1441 gtaaagccct cgccacgttc aatcgcagct gaagagcctg ccacgccttc caacatcgcc
     1501 acatcagagc ctgcagcgat ggatttgagc atgtattcac ctgccaactt gccacctgcg
     1561 acgttgtcag aggcgatgtg agaaatcact ttgccgccgc ctgctgagcg gtctacggta
     1621 atcacaggga tgcctgcttt gttggcagat tcaatcgcag acgcgacggc agcagaatcc
     1681 acgggattga tgatgatgag tttcacgcct tgttgaatca agtcttccac gtcggccgct
     1741 tgttt
//
LOCUS       DAOMVK010000493         1745 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_19936_length_1745_cov_2.685799, whole genome shotgun sequence.
ACCESSION   DAOMVK010000493 DAOMVK010000000
VERSION     DAOMVK010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19936_length_1745_cov_2.685799"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..863
                     /locus_tag="PK856_12025"
     CDS             60..863
                     /locus_tag="PK856_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRL03138.1"
                     /translation="MAKIHTFKAGFCTHAACMAVRGAGFAQCEFPAQVFLLEAAGRFW
                     LFDTGYAHHFLDATASGLFALYRKVTPVVFNESEAMRLQLREFGVNVADLSGVIVSHF
                     HGDHIGGLKDFPHMPIIACGAGWQQVRSLRGVRALLQGFVPAVMPEDTNQRLLAMESF
                     ERVALPSDCAPLQAAYVLPHSQGEILLVDLPGHAAGHMGALVACDDGWHLLAADAAWS
                     RANYRDWKLPSPLSYLIMDKSSAFKHTLGQLQQLDARGKVRIHLSHEEV"
     gene            865..>1745
                     /locus_tag="PK856_12030"
     CDS             865..>1745
                     /locus_tag="PK856_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoF synthetase"
                     /protein_id="HRL03139.1"
                     /translation="MALWPLLQAYWKSKRLRFQARADLERHQAQQWQRFEQQVLAKSR
                     YFQPFLGQPLMAWPLMNKAIMLQHFNAMNTVGMDLQAAFAVAQRAEQERDFAPTLNGI
                     SVGLSSGTSASRGVFVVSPAEQARWAGTILAKLLPQGLLSGERVALVLRANNNLYERI
                     DNRFIAFRFFDLLQAFDDIASQLQAYRPSIIVAPAQVLRALALAQQQGRIDLQPKRVI
                     SAAEVLSDADRAIIETSFPELHQVYQATEGFLAVTCEHRRLHLNEEFLMVEQEWLDDT
                     RYVPIITDFTRSSQPIIR"
BASE COUNT          368 a          441 c          506 g          430 t
ORIGIN      
        1 gttgacatgc agcaagcgat tttaaacacc gctgaaaact tcagacactt gggataaaca
       61 tggccaaaat ccacaccttc aaagccggtt tttgcaccca tgcggcctgt atggcggtgc
      121 gcggtgcggg gtttgcccag tgtgagtttc ctgcacaagt gtttttattg gaagcagcgg
      181 ggagattttg gctgtttgac acgggctatg cccaccattt tctcgatgcc accgcttcag
      241 gcttgtttgc gctgtatcgc aaagtcacgc cggtggtgtt taacgaaagc gaggcgatgc
      301 ggctgcaatt gcgtgaattt ggtgtgaatg tggccgattt gagcggcgtg attgtgtcgc
      361 attttcacgg cgaccacatc ggcggcctga aagattttcc acacatgccc atcatcgcct
      421 gtggtgcagg ttggcaacag gtgcgcagct tgcgcggtgt gcgagctttg ttgcaaggct
      481 ttgtgcccgc ggtgatgcct gaagacacca accaacgctt gctggcgatg gaatcgtttg
      541 aacgcgtggc cttgcccagt gattgtgcgc ctttacaggc cgcttatgta ttgccacaca
      601 gccaaggcga gattttattg gtggatttgc ctggccatgc cgcagggcac atgggcgcat
      661 tggtggcgtg tgacgacggt tggcatttgc tggcggccga tgccgcttgg agccgcgcca
      721 attaccgcga ttggaaactg ccgtcgcctt tgagttacct catcatggac aaatcttccg
      781 ctttcaaaca cacgctggga caattgcaac aactggatgc gaggggcaaa gtacgcatcc
      841 atttgagtca cgaggaggtg tgagatggca ttgtggcctt tgttacaagc atattggaaa
      901 agcaaacgct tgcgttttca agcccgtgcc gatttggagc gccaccaagc gcaacaatgg
      961 cagcgttttg agcagcaagt gttggccaaa agccgctatt ttcagccttt tcttggccag
     1021 ccgctgatgg catggccgtt gatgaacaaa gccatcatgt tgcaacactt caatgccatg
     1081 aacacggtgg gcatggattt acaggccgct tttgccgtgg cgcagcgggc ggagcaagag
     1141 cgcgattttg cgcccactct taatggcatc agcgtgggac tgtcttcggg cacatcggcc
     1201 agtcgcggcg tgtttgtggt cagccctgct gagcaagcgc gttgggcggg cacgatattg
     1261 gcaaagttgt tgccacaagg tttgctcagt ggcgagcgcg tggctttggt tttgcgtgcg
     1321 aacaacaatt tgtatgaacg catagacaac cgcttcattg cgtttcgatt ttttgatttg
     1381 ctgcaagcgt ttgacgacat tgcctcgcaa ttgcaagctt acaggccgag catcatcgtg
     1441 gcgcctgcgc aagtcttgcg cgcactggcc ttggcgcagc agcaaggacg cattgattta
     1501 cagcccaaac gggtgatttc ggcggcagaa gtattgagcg atgccgaccg cgccatcatc
     1561 gaaacgtctt ttcctgagtt gcaccaagtc taccaagcca ccgaaggctt tttggcggtg
     1621 acatgcgaac acaggcgttt gcatttgaat gaagaatttt tgatggtaga gcaagaatgg
     1681 ctagacgaca cgcgttacgt gcccatcatc accgacttta cccgcagctc gcagcccatc
     1741 atccg
//
LOCUS       DAOMVK010000494         1742 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_19979_length_1742_cov_3.578542, whole genome shotgun sequence.
ACCESSION   DAOMVK010000494 DAOMVK010000000
VERSION     DAOMVK010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19979_length_1742_cov_3.578542"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1742
                     /locus_tag="PK856_12035"
     CDS             <1..>1742
                     /locus_tag="PK856_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HRL03140.1"
                     /translation="GDIKGKVGEHLQAALDHLPLSKQLITIKTDMDFSQELPNHLADL
                     SPKSPNWADLVTRFTELGFKRWLAEAEQHASKGTTDDLFAPAPAAPVAKAAPAPTTAP
                     KIEHQLGNEIERQYQTILTEDALQNLLQRLQAALDSEQAIAIDTETTSLSAMDAQLVG
                     ISIAFGAGDAVYIPLGHTVTAAPEQLDLDATLSALKPYLENSKLGKIGQNIKYDRHIF
                     ANYAIQLQGVVADTMLLSYVLESHLGHGMDELAERHLGITTVPYESLCGKGAKQISFA
                     DVALDDAAHYAAEDADITWRLNAVLLPQLDAKQRELYEQVELPVEHVLWQMERTGVLI
                     DKAELDKQSHELGSQILALENEAYQLAGQPFNLNSPKQLQEILFGKLAIPTTGLKKTA
                     SGGISTNEAVLEKLAPNYDLPKLILQNRSLTKLKSTYTDKLPQLIRADTGRVHTTYAQ
                     AVAITGRLASNNPNLQNIPIRTAEGRCVRQAFIAPAGHKIISADYSQVELRIMAHLSG
                     DATLIDAFNKGEDVHRRTAAEVFHVSPEDVSSEQRRYAKTINFGLIYGMGQYGLANNL
                     EITPAEAQEFINRY"
BASE COUNT          474 a          489 c          416 g          363 t
ORIGIN      
        1 ggcgacatca aaggcaaggt gggcgagcat ttacaggccg cattagacca tttgccgctg
       61 tccaaacaac tcatcaccat caaaaccgac atggatttca gccaagagct gccgaatcat
      121 ttggccgatt tgagccccaa atccccaaac tgggcagact tggtaacccg ttttaccgaa
      181 ttgggcttca agcgctggct ggccgaagcc gaacagcatg cgagcaaagg cacaacagac
      241 gatttgtttg cacccgctcc cgcagcgcct gtggccaaag ccgctcccgc tcccaccact
      301 gcccccaaaa ttgaacacca attgggcaat gaaatcgagc gccaatacca aaccattctg
      361 actgaagatg cgttgcaaaa cctgttacag cgtttacagg ccgcattaga cagcgaacaa
      421 gccatcgcca tagacaccga aaccactagc ttaagcgcca tggatgcgca attggtaggc
      481 atttccatcg cttttggtgc aggcgatgcg gtttacattc ctttgggcca taccgtcact
      541 gccgcgcctg aacaattgga tttagatgcc actttaagtg cgctcaagcc ttatttggag
      601 aattcaaaac tgggcaaaat cggccaaaac atcaaatacg accgacacat ttttgccaat
      661 tacgccatcc aattacaggg cgtggtagca gacaccatgt tgctgtcgta tgtattggaa
      721 agtcatttgg gccacggcat ggacgaattg gccgagcgcc atttgggcat caccaccgtt
      781 ccttacgaaa gcctgtgcgg caaaggcgcg aagcaaattt catttgccga cgtggctttg
      841 gacgatgcgg cgcattatgc cgctgaagat gccgacatca cttggcgctt gaatgcggtg
      901 ttgttgccgc aattggatgc caaacagcgc gagttgtatg aacaagtgga attgcccgta
      961 gaacacgtgt tgtggcaaat ggagcgcacg ggcgtactca tagacaaagc cgaattggac
     1021 aagcaaagcc acgaactggg ttcgcaaata ttggccttgg aaaacgaggc ttaccaattg
     1081 gcaggccaac cgtttaacct caattcgccc aaacaattgc aagaaattct gtttggtaaa
     1141 ctggcgatac cgaccacagg cttgaaaaaa actgcctcag gcggcatttc caccaatgaa
     1201 gcggtgttgg aaaaactggc gccgaattac gatttgccca agttgattct gcaaaaccgc
     1261 agcctgacca agctcaaatc cacctatacc gacaaattgc cgcagttaat tcgtgccgac
     1321 acaggtcgcg ttcacaccac gtatgcacaa gcggtggcga ttacaggccg cttggccagt
     1381 aacaatccca atttgcaaaa cattcccatc cgtactgccg aaggccgctg tgtgcgccaa
     1441 gcctttatcg cacccgctgg ccacaaaatc atctcggccg actactcaca agtcgaattg
     1501 cgcatcatgg cacacctgtc tggcgatgcc acattaatag atgcatttaa caaaggcgaa
     1561 gacgtgcacc gccgcaccgc cgccgaggtg tttcatgtct cccccgaaga cgtgagcagc
     1621 gagcagcgcc gctacgccaa aacgattaac ttcggcttaa tctacggcat gggccaatac
     1681 ggcttggcca acaatttgga aatcacccca gccgaagcgc aagaattcat caaccgctat
     1741 tt
//
LOCUS       DAOMVK010000495         1738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_20056_length_1738_cov_3.809863, whole genome shotgun sequence.
ACCESSION   DAOMVK010000495 DAOMVK010000000
VERSION     DAOMVK010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20056_length_1738_cov_3.809863"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="PK856_12040"
     CDS             complement(<1..347)
                     /locus_tag="PK856_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRL03141.1"
                     /translation="MANKNLFAKRGANKADAYNEAGGVAYQLSPQAQLAQLAATGCLN
                     HTYYTSAEDQLSKVLELTQDLPAEFIAKTALFARKQAHMKDMPALLLAVLAQKDVAMC
                     AQVFGAVIDNGKM"
     gene            555..>1738
                     /locus_tag="PK856_12045"
     CDS             555..>1738
                     /locus_tag="PK856_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003798794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA repair transcriptional activator RtcR family
                     protein"
                     /protein_id="HRL03142.1"
                     /translation="MKKQRVLMGFMGTVLDGGFNAKRWERWRPSVNVHQTEGSDIDVV
                     ELFHDDKHEKQALVVRADIHSVNPDAAIHLHEMNIDNPWDFSEVYGKLFDWAQRYPFD
                     TENHEYWVHITTGTHVVQICLFLLVEARVIPGVLLQTSPPKDKRGVSEQDKGRFEVID
                     LDLARYDDIAARLSLAQTDAQRFLKSGIATRNQRFNHMIAEIEQVAISSPSPILLNGA
                     TGAGKSMLAKRIYELKKARHLVSGRFVDVNCATLRGDMAAAALFGHGKGAFTGAANSR
                     AGFLKSADLGVLFLDEIGELGMDEQAMLLKAIEEKEFFAMGSDTPSSSQFQLIAGTHK
                     DLRAEVAAGRFREDLYARINIWQYTLPSLAERREDIEPNIEQQLLWVGQELGRFVRFN
                     QEA"
BASE COUNT          415 a          400 c          471 g          452 t
ORIGIN      
        1 aacatcttac cgttatcgat gaccgcacca aacacttggg cacacatggc cacgtctttt
       61 tgggccaata ccgccagcaa caaagcaggc atgtctttca tgtgcgcttg tttgcgggca
      121 aacaaagcgg ttttggcgat gaattcggca ggcaaatctt gggtcaattc caacactttg
      181 ctcaattggt cttcagcgct ggtgtagtaa gtgtggttca agcaacccgt tgcggcaagt
      241 tgcgccaatt gtgcttgtgg gctcaattgg taagcaaccc caccggcttc attgtaagca
      301 tcggccttat tggcaccgcg cttggcaaat aggtttttgt tggccatctc atcactcctg
      361 ctgtgtattt ttcgttgttt cttgctgttt accatcgcaa gcatcgtgcc aagcccgtat
      421 tttatttttc aatttgttct taattgattg aatttaaatg aaaattattt ttaaaattct
      481 tattttacat cttgacgcct gtttgagttt tctgataaaa ctatccaata aaatacaaat
      541 atagaaaatt atctatgaaa aaacagcgtg tgttgatggg atttatgggt acggtgctcg
      601 atggtggctt caatgccaaa cggtgggagc gttggcggcc gagtgtgaat gtgcaccaaa
      661 ccgagggcag tgacattgat gtggtggagt tgtttcatga tgacaagcat gaaaaacaag
      721 ccttggtggt gcgcgccgac atccacagcg tcaatcccga tgccgccatc catctgcatg
      781 aaatgaacat agacaatccg tgggattttt ctgaggtata cggcaagctg tttgattggg
      841 cgcagcgcta cccatttgac actgaaaacc atgaatattg ggtacacatc accacgggca
      901 cgcacgtggt gcaaatttgt ttgtttttat tggtggaggc gcgagtcatt cctggggtgt
      961 tgttgcaaac ctcgccaccc aaagacaagc gcggcgtgtc tgagcaagac aaggggcgat
     1021 ttgaagtcat tgatttggat ttggcgcgct atgacgacat tgccgcgcgg ctgtctttgg
     1081 cgcaaaccga tgcgcagcgc ttcttaaaaa gcggcattgc cacgcgcaat cagcgtttca
     1141 accacatgat tgccgaaatc gaacaggtgg cgatttcgtc gccatcgccg attttgctca
     1201 atggcgcgac gggcgcaggc aaatccatgc tcgccaagcg catttatgaa ttgaaaaaag
     1261 cccgccattt ggtatcgggc cgctttgtcg atgtcaattg cgccactttg cgcggcgaca
     1321 tggcggcggc cgctttgttt ggccatggca aaggcgcgtt tacaggcgct gccaacagcc
     1381 gcgcaggctt tctcaaaagc gctgacttgg gggtgttgtt tctggatgaa atcggcgaat
     1441 tgggcatgga tgagcaggcg atgttgctca aggccatcga agaaaaagaa tttttcgcca
     1501 tgggcagcga cacgcccagc agcagccaat tccaactgat tgcgggcaca cacaaagact
     1561 tgcgcgctga agtggcggcg gggcgatttc gtgaggattt gtatgcgcgc atcaatattt
     1621 ggcaatacac gctgccaagc ttggccgagc ggcgcgaaga catagagccg aacattgagc
     1681 aacaattgct gtgggtgggg caggaattgg ggcggtttgt gcgcttcaat caagaggc
//
LOCUS       DAOMVK010000496         1730 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_20191_length_1730_cov_3.318806, whole genome shotgun sequence.
ACCESSION   DAOMVK010000496 DAOMVK010000000
VERSION     DAOMVK010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20191_length_1730_cov_3.318806"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="PK856_12050"
     CDS             complement(<1..356)
                     /locus_tag="PK856_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HRL03143.1"
                     /translation="MKHLFRLGVDAGGTFTDFILSDAHGKVLLCKSLSTPQDGTLAIA
                     DGLRQLAEHTGLSVEELVQSCDLCINGTTVCLNALIELKGVKVGLLCTAGHEDSIEIR
                     LGHKEEGYRYDASYPPA"
     gene            complement(375..>1730)
                     /locus_tag="PK856_12055"
     CDS             complement(375..>1730)
                     /locus_tag="PK856_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009118791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HRL03144.1"
                     /translation="PAGYNPDAKELYAEGLRIPPVKLWDKGVRRDDVINLLMCNMRSR
                     RNQEGDLNAQLGACRVGERNLLNLLDKYGLDTVQAAIAELKNMADHHMRDLIRSIPDG
                     SYHGEAVLEDSGHGLGAMTISADINITGDSVHIRIDSPPQVPYFINSYAGNSMSGVYL
                     GLMMFAQVPPPYNEGLYRCVTVDLGEKATLCNAQEPAPHVNCTTTPMETLTDAVRKAF
                     EAAAPERVTASWGHASGINIAGIDPRTGEQYVTMVLASIISGAGATQQMDGWHACGPL
                     CCFGALSSGDIELLEYQYPILIHKYGLAQDSGGAGRYRGGSGTVWEVEPLGHEMTVIA
                     FGEGRQIPTMGAADAENGLHEPKLGRLEIVHGADNTTVYTQNTLTHIQPGQRARNTNP
                     GGGGYGIAFERPVNAVLHDYVQGLITLEGARLDYGVVIIDGEVDITATDAVRATQNIT
                     A"
BASE COUNT          369 a          468 c          481 g          412 t
ORIGIN      
        1 gcgggtgggt aagaggcatc gtagcggtag ccttcttctt tgtggcccaa gcggatttca
       61 atgctgtctt catggcctgc ggtacagagc aagcccactt tcacgccttt gagctcaatc
      121 aaggcattca agcacacggt ggtgccgttg atgcacaaat cacagctttg caccaattct
      181 tctaccgata aacctgtgtg ttcggccaat tggcgcaagc catcggcaat ggccaaagtg
      241 ccgtcttgtg gggtagacaa actcttgcac agcaagactt tgccatgggc atcggacaaa
      301 ataaagtcgg taaacgtgcc gccagcatcc acgcccaagc gaaataaatg tttcatgtca
      361 gttccttttt gcgtttatgc agtgatgttt tgggtggcac gcacagcgtc ggtggcagta
      421 atgtctactt caccatcgat gatgaccacg ccatagtcca agcgtgcgcc ttctaaggta
      481 atcaagcctt gcacataatc gtgtaatacc gcgtttacag gccgttcaaa ggcgatgcca
      541 taaccgccac cgcctggatt ggtgttgcgg gcgcgttgac ccggttggat gtgggttaag
      601 gtgttttggg tgtaaacagt ggtgttgtcg gcaccgtgca caatttccaa gcggcccaat
      661 tttggctcgt gcaagccgtt ttcggcatcg gccgcgccca tggtggggat ttggcggcct
      721 tcaccaaagg caatcaccgt catttcgtga cccaaaggct ccacttccca taccgtgcct
      781 gagccgccgc gatagcgacc tgcgccgccg ctgtcttggg ccaagccgta tttgtgtatc
      841 aaaattgggt attggtattc gagcaattcg atgtcgccag acgacaaagc accaaagcaa
      901 cacaaaggcc cacacgcatg ccagccgtcc atttgttggg ttgcgccagc gcctgaaatg
      961 atggatgcca acaccatggt cacgtattgc tcgcctgtgc gcgggtctat gccagcgatg
     1021 ttgatgccag aagcgtggcc ccaagaggcg gtgacgcgtt ctggcgcggc ggcttcaaag
     1081 gctttgcgca cggcatcggt cagggtttcc attggtgtgg tggtgcaatt cacgtgcggt
     1141 gcaggctctt gtgcgttgca cagcgtggct ttttcaccca aatcaacggt gacgcagcgg
     1201 tacaagcctt cgttatacgg cggcggcact tgggcaaaca tcatcaagcc caaatacacg
     1261 cccgacatcg aattgcctgc ataagaattg atgaaataag gcacttgtgg cgggctgtcg
     1321 atgcggatat gcacgctgtc gcccgtgatg ttgatgtcgg ccgaaattgt catcgcaccc
     1381 aagccgtgac ccgaatcttc cagcaccgct tcgccgtggt aagagccatc aggaatggag
     1441 cgtatcaagt cgcgcatgtg gtggtcggcc atgtttttca attcggcaat ggcggcctgt
     1501 acggtgtcta agccgtattt gtcgagcaaa ttgagcaaat tgcgttcgcc cacacggcac
     1561 gcacccaatt gcgcattcaa gtcgccttct tggttgcggc gtgagcgcat gttgcacatc
     1621 agtaaattga tcacgtcatc gcggcgcacg cctttgtccc acagcttcac aggcggaatg
     1681 cgcaagcctt cggcatacaa ttctttcgcg tctgggttat agcctgcggg
//
LOCUS       DAOMVK010000497         1730 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_20201_length_1730_cov_1.872239, whole genome shotgun sequence.
ACCESSION   DAOMVK010000497 DAOMVK010000000
VERSION     DAOMVK010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20201_length_1730_cov_1.872239"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="PK856_12060"
     CDS             <1..387
                     /locus_tag="PK856_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03145.1"
                     /translation="HKITFEEYVNKLHGRVVFDGGRAGFLDEYFEAEGYKRQVKATFF
                     NYSTAVNAIIGSPLLLTMPTYAAHTYAENNVKLAVHPVPLILAPSFVVEAIWDIKKQT
                     DLRNLWLRDLVVNVTAETRTNLRSNQ"
     gene            609..1676
                     /gene="tauA"
                     /locus_tag="PK856_12065"
     CDS             609..1676
                     /gene="tauA"
                     /locus_tag="PK856_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_function: GO:0030977 - taurine binding [Evidence IEA];
                     GO_process: GO:0015734 - taurine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HRL03146.1"
                     /translation="MAKAASKKITAVVAALAVAGIAFFIISQNGNANTAPSVASKDGA
                     ASASKSTAKPIVVAYQTGVDPTKVAQANGDYEAATGANIEWRKFDSGAEVIAAVTSGD
                     VPVGNIGSSPLAAAASQNVPIEVFYISGVIGQSEALVVSNKISKPEDLVGKKVAVPFV
                     STTHYSLLSALKHWNIDAKKVDIVNLRPPEITAAWKRGDIDAAYVWEPALSQAKQTGK
                     VLVSSEEVGKWGAPTYDLWIVRKDFAEKNPEFLKQFAQVTGKQVDAYNQDPQGYAANA
                     DNVSKIAKLTGSKPEDVALLLSGNRYPNLAEQATILGGSFAEDVKKTAEFLKEQGKVD
                     AVKASYQENVTAKFVQAAAGK"
BASE COUNT          488 a          365 c          439 g          438 t
ORIGIN      
        1 cacaaaatta cttttgagga gtatgtgaat aaattgcatg gaagagtggt ttttgatgga
       61 ggacgtgcag gatttttgga tgagtacttt gaggctgaag gctataaacg acaagtaaaa
      121 gccacttttt tcaattattc tactgccgtc aatgccatta ttggtagtcc actgttattg
      181 accatgccaa cctatgctgc acatacctat gctgaaaata atgttaagtt ggcggtgcac
      241 ccagtcccat tgatattggc gccttcattt gtggtagagg cgatttggga cattaaaaag
      301 caaactgatt tacgcaatct gtggttacgt gatttggtcg tgaatgttac agcagaaacg
      361 agaacgaatt taagatccaa tcaatgacag cgattaagtg tttaaatggg gcaataaagt
      421 cgataaaaca aatatgcgca tcatcatgga tgaaacagaa gttgcatttg gcttcgtttg
      481 aactttgtta caggccgcat gtggtttatt taattctttt aagttatttg taaatcgttt
      541 tatattcttt ctgattttat cgactttctt ctatgatgtt tttaacaaaa cattacggga
      601 gaaacaccat ggccaaagct gcttctaaaa aaattaccgc cgttgttgcc gccttagcgg
      661 tggcaggcat cgcgtttttc atcatcagtc aaaatggcaa cgccaatacc gcgccaagtg
      721 ttgcgagcaa agacggcgca gcatccgcaa gcaaatccac agccaagccc attgtggtgg
      781 cttaccaaac cggggtagac ccgaccaaag tggcgcaagc caatggcgac tatgaggctg
      841 cgacaggcgc gaacattgaa tggcgcaaat ttgacagcgg cgccgaagtc atcgccgcgg
      901 tgacttcagg tgatgtgcct gtgggcaaca tcggttccag tcctttggcg gcggcggcca
      961 gtcagaatgt gccgattgag gtgttttaca tttcaggtgt gatagggcag tcagaggctt
     1021 tggtggtgag caataaaatc agcaaacctg aagatttggt tggcaagaaa gtggcggtac
     1081 cgtttgtttc caccacccat tacagcctgt tgtcggcctt gaaacactgg aacatcgatg
     1141 ccaaaaaagt cgatattgtc aatttgcgtc caccagaaat caccgcggca tggaagcgtg
     1201 gcgacattga tgccgcttat gtgtgggaac ctgcgctgag ccaagccaag caaacgggca
     1261 aggttttggt gagcagcgaa gaagtgggca aatggggcgc gccaacttac gatttgtgga
     1321 ttgtgcgcaa ggattttgcc gagaaaaacc ctgaattttt gaaacaattt gcccaagtca
     1381 caggcaaaca agtcgatgcc tacaatcaag acccgcaagg ctacgctgcc aatgccgaca
     1441 atgtcagcaa aatcgccaaa ctgacaggct ctaagcctga agacgtggcc ttgttgcttt
     1501 caggcaatcg ctaccccaat ttggctgaac aagcgaccat attgggcggc agttttgccg
     1561 aagatgtgaa gaaaaccgca gaatttttga aagaacaagg caaggttgat gccgtcaaag
     1621 ccagttacca agaaaatgtc actgccaagt ttgtgcaagc cgcggcgggt aaataatgat
     1681 gtcggattta catttacaac aggtgtcggt gcaatacgca ggtcagcatg
//
LOCUS       DAOMVK010000498         1727 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_20244_length_1727_cov_2.804426, whole genome shotgun sequence.
ACCESSION   DAOMVK010000498 DAOMVK010000000
VERSION     DAOMVK010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20244_length_1727_cov_2.804426"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /gene="queA"
                     /locus_tag="PK856_12070"
     CDS             complement(<1..655)
                     /gene="queA"
                     /locus_tag="PK856_12070"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HRL03147.1"
                     /translation="MHINDFDFELPEALIAQHPPEIRGSSRLLAAWQQQDLQDLQFND
                     ISQFFEAGDVLVMNDTKVIKARLFGQKQSGGKIEVLVERVINDHEVWAHIRSSKSPKQ
                     NSVVLFGDEQATMRERKGELFVLEFTGEKTVWEVLEAQGHMPLPPYIERADDEEDDER
                     YQTVYAQIDGAVAAPTAGLHFTPELMQALKDKGVDIHYVTLHVGAGTFQPVRVDNIAE
                     "
     gene            901..1341
                     /gene="aroQ"
                     /locus_tag="PK856_12075"
     CDS             901..1341
                     /gene="aroQ"
                     /locus_tag="PK856_12075"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HRL03148.1"
                     /translation="MSEILVLHGPNLNLLGSREPEIYGATTLADINAKLTDLAASANL
                     SIDTLQSNAEYVLLDRIHAARTEGTRMILINPGAFTHTSVALRDALTGVAIPFLEIHL
                     SNVHAREPFRQHSYLSDVAKGVICGLGVHSYYLAMQYAIATLKA"
     gene            1358..>1727
                     /gene="accB"
                     /locus_tag="PK856_12080"
     CDS             1358..>1727
                     /gene="accB"
                     /locus_tag="PK856_12080"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HRL03149.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEDKVRVTRTYAAPAATYA
                     APAPTYAAAPVAAAAPVATIVSEAATPVVAAANALKSPMVGTFYRSASPTSAAFVEEG
                     QTVKVGDTLCIIEAMKLMNEI"
BASE COUNT          447 a          457 c          378 g          445 t
ORIGIN      
        1 gctctgcaat attgtccacg cgcacaggct gaaacgtacc cgcgcccacg tgcaaagtca
       61 cgtaatggat gtccacgcct ttgtctttta aggcttgcat caattcgggc gtgaaatgca
      121 aacccgcggt tggggccgcc acagcgccat cgatttgcgc gtacacggtt tggtagcgct
      181 catcgtcttc ttcatcgtcg gcgcgttcta tataaggcgg caaaggcatg tgaccttgcg
      241 cttccaacac ttcccacacg gttttttcac ccgtgaattc cagcacaaac aattcgcctt
      301 tgcgctcacg catggtggct tgttcgtcac caaacaacac caccgagttt tgttttggcg
      361 attttgaaga gcggatgtgt gcccacactt cgtggtcgtt aatcacccgc tcaaccaaca
      421 cttctatctt tccgccactt tgcttttgac caaacaaacg tgctttgatg actttggtgt
      481 cattcattac cagcacatcg cccgcttcaa aaaattgact tatgtcattg aattgcaaat
      541 cttgtaagtc ttgctgctgc cacgcagcca acaagcggct gctgccacga atctcgggag
      601 gatgctgtgc aatcagcgct tcagggagtt caaaatcaaa atcattaatg tgcatggtca
      661 ctgctttaaa acaacaaagg gcgcattata cgcgcatgta gacaaaatag cggcctgtaa
      721 aactgtagcg acactcgaat aattcatggc acagcaccgc acaaaacagt acaatgcgca
      781 tctttataac cgtggtgtct ttacttcaat cgcaaaaaac aacactcctt ttatctgcaa
      841 tactggactg ttagccgaaa ttgagtaaaa atgccgtcat tactatcacg aaaccactgt
      901 atgagcgaaa tattggtctt gcacggcccc aacctgaatt tattgggtag ccgcgaacca
      961 gaaatctatg gtgccaccac tttggcagac atcaatgcca aacttaccga cctagccgct
     1021 tctgccaatc tcagcataga cactttacaa agcaatgccg aatacgtgtt gcttgaccgc
     1081 atccatgctg cgcgcacgga aggcacacgc atgattttaa tcaatcctgg tgcgtttacc
     1141 cacaccagcg tggccttacg tgatgcctta acgggtgtcg ccataccatt tttagaaatt
     1201 catttgtcca atgtgcatgc acgtgaaccg tttcgtcagc actcatattt atcagatgtg
     1261 gccaagggcg tgatttgtgg cttgggggta cacagttatt acctcgccat gcaatacgcc
     1321 atcgccacat taaaagcata ataaaaagga agcttccatg gatttacgta aacttaaaaa
     1381 attaatcgac ttggttgaag aatcaggcat tgctgaaatt gaagtgaccg aaggcgaaga
     1441 caaagtccgc gtaacccgca cttatgccgc ccctgctgcc acttacgcag cgcctgctcc
     1501 tacttatgct gcggctccag ttgctgctgc agctccagtt gcgactattg ttagcgaagc
     1561 cgctacgcca gtggttgctg ctgccaatgc cttgaagtcc cctatggtag gtacgttcta
     1621 ccgctcagcc agccctactt ctgcggcttt tgttgaagaa ggccaaacgg ttaaagtggg
     1681 tgataccttg tgcatcatcg aagcaatgaa attgatgaat gaaatcg
//
LOCUS       DAOMVK010000499         1723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_20320_length_1723_cov_1.744604, whole genome shotgun sequence.
ACCESSION   DAOMVK010000499 DAOMVK010000000
VERSION     DAOMVK010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20320_length_1723_cov_1.744604"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..>599
                     /locus_tag="PK856_12085"
     CDS             24..>599
                     /locus_tag="PK856_12085"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HRL03150.1"
                     /translation="MKSGQLLAFSSNYELYADLSSRMMQAIATLVPDTEIYSIDECFA
                     DVRGMDALRDLGLSLKARVWQWVGIPTCVGIAPTKTLAKLCNHLAKRYPNHFSGVVVW
                     NDWNQDIQRRAMQSTAVTDLWGIGRRIGVSLAEQGIQSVWDFHQADTQTLRRQFGVVI
                     ERTQRELQGLRCQDLNPAEANKHNLIRSRSFG"
     assembly_gap    600..685
                     /estimated_length=86
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <686..1151
                     /locus_tag="PK856_12090"
     CDS             <686..1151
                     /locus_tag="PK856_12090"
                     /inference="COORDINATES: protein motif:HMM:NF023227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
                     /protein_id="HRL03151.1"
                     /translation="HISSGAEALRAQHSQANTISVFIHTNRFREQDPQYRGYHMVAVP
                     QASADTLYLNHIAQAVLKRLFRTGYSYKKCGIELSGIESSQNHQQQDFWANGDNQKSK
                     AVMEVLDQVNRSFGRGALKLGSEALADNWKMNRNLLSPCFTTRFKDLPKIGE"
     gene            1208..>1472
                     /locus_tag="PK856_12095"
     CDS             1208..>1472
                     /locus_tag="PK856_12095"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - cellular response to DNA damage
                     stimulus [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HRL03152.1"
                     /translation="MGLQQDWVSPFDTPHVFNHQLAPMILPSPDGVGEPNQLMVAEFG
                     LLPSWSKERHIKYATMNARYESVVTKPAFKNAWFKGQKCIVPAD"
     assembly_gap    1473..1499
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1500..>1723
                     /locus_tag="PK856_12100"
     CDS             <1500..>1723
                     /locus_tag="PK856_12100"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - cellular response to DNA damage
                     stimulus [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HRL03153.1"
                     /translation="IVEPDWRTGKHIPAHISRLDGKPMGIAGLWDYWTDALTGEVVFS
                     FTLLTINADEHEFMRRFHKPQDEKRMVVIL"
BASE COUNT          446 a          369 c          401 g          394 t
ORIGIN      
        1 cgtattttca ggtacagcac ttaatcaaaa gtgggcaact ccttgccttc agcagcaatt
       61 atgaattgta tgccgactta tcatcacgaa tgatgcaagc cattgcgacc ttggtacctg
      121 acaccgaaat ctattccata gacgaatgtt tcgcagatgt ccgcggaatg gatgctttgc
      181 gcgatttggg cctgtctctc aaagcaaggg tttggcaatg ggtgggcata ccaacctgtg
      241 tgggtatcgc gccaaccaaa actttggcca aactctgcaa ccatttggcc aaacgttatc
      301 ccaaccattt cagtggcgtt gtcgtttgga atgattggaa tcaggacatt caacgccgtg
      361 ccatgcaaag cacagcagtg actgatttgt ggggaatagg ccgtcgcatc ggtgtcagtt
      421 tggcagagca gggcatacag tcagtctggg actttcatca ggccgataca cagactttgc
      481 gtcgccaatt tggagtggtg attgaacgca ctcaaagaga attacaaggc ttgcgttgcc
      541 aggatttaaa ccctgcggaa gccaataaac acaatttgat acgcagccgc agtttcggcn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnccaca tcagcagtgg ggccgaagcc ttaagggcac
      721 aacacagtca agccaatacc atcagtgtgt tcatacacac caatagattt cgggagcaag
      781 atccccaata ccgtggttac catatggtgg cggttcctca agccagcgcg gataccctgt
      841 acctgaatca cattgcgcaa gcggtattga agcgcttgtt tcgaacaggc tattcgtata
      901 aaaaatgcgg cattgagcta agtggtatcg aatcgtctca aaatcaccaa caacaagatt
      961 tctgggcgaa tggtgacaat caaaaatcaa aagcagtgat ggaagtgctg gaccaggtca
     1021 atcgcagctt tggcagaggg gcactgaagt tgggcagcga agccttggct gacaattgga
     1081 aaatgaatcg caacctgtta agcccgtgct tcactacccg ttttaaagac ttgcccaaaa
     1141 ttggagaata agcatgtgct caaattttta tcccacccta gacccacaac ggttaaatgt
     1201 atttgcattg ggtttacaac aagattgggt aagtccgttt gatacaccac atgtgtttaa
     1261 ccatcagctg gcaccgatga ttctgccatc tccagatgga gtgggtgagc ccaaccaatt
     1321 gatggtggcc gagtttgggt tgttaccgtc ttggtccaag gaacgtcata tcaaatacgc
     1381 aaccatgaat gcacgttatg agagtgtggt caccaaaccc gcttttaaga acgcatggtt
     1441 caaagggcag aaatgcattg ttccagcaga tgnnnnnnnn nnnnnnnnnn nnnnnnnnna
     1501 ttgtggaacc tgattggcgt acaggtaagc acatcccagc gcacatatct cgccttgatg
     1561 gcaaaccaat ggggattgct ggattatggg attattggac agatgcatta acgggtgagg
     1621 ttgtgttcag ctttaccctg ctgaccatca atgcggatga acatgaattc atgcgacggt
     1681 tccacaaacc acaagatgaa aagcgcatgg tggtcatttt gga
//
LOCUS       DAOMVK010000500         1719 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_20372_length_1719_cov_2.523438, whole genome shotgun sequence.
ACCESSION   DAOMVK010000500 DAOMVK010000000
VERSION     DAOMVK010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20372_length_1719_cov_2.523438"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PK856_12105"
     CDS             complement(<1..387)
                     /locus_tag="PK856_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03154.1"
                     /translation="MKIKKLWQMPPETYTFKSHEQPAMIGSPGVPDHPHRRKVFYALV
                     GIVLALTGGLQNGLLTAVLPQLQGFLALTPAEGGWILVTYNMVNACMSMLLFKCRQQF
                     GLARFIRAIIFALVLCNLIQVTTHSYH"
     gene            complement(641..>1719)
                     /locus_tag="PK856_12110"
     CDS             complement(641..>1719)
                     /locus_tag="PK856_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03155.1"
                     /translation="QPDKNMLHTLMQAWKGQAATWLVEIMSTWTRRLGNFATFAQLQL
                     WLQEGQWQNLPARIAQDCSRQLVQNHCTPEFLQAAFTERVAKLSPVQWQQLSQHAADT
                     LGRLYHRNIKPLGKVLTEFSRQHEYVTVLYPQSMQQDHAIIGLITDTVKRQQAHVSGL
                     ASPCVDRISIVLSSTCYLQEWHGYAAMMAAYRLTMKDDKTLWYWHVDGELASLRVMMA
                     ANNVRNRDLIRQQLLLAQVCEYLRFEQGQCGLYLDNQAEPIMFTASNLTELVEQMPLP
                     AVRQLLLHNRALRDEQGWPTSVNVSKQLDAVLADFRQQILPEGVDVADADWQDAGRYV
                     VWVRAVQQIKQHWLRAPAGVTVAN"
BASE COUNT          403 a          463 c          452 g          401 t
ORIGIN      
        1 gtggtagctg tgggtggtaa cttggatgag gttgcacaag accaaggcaa agatgatggc
       61 gcggatgaat ctggccaagc caaattgctg ccgacacttg aacaacaaca tgctcatgca
      121 ggcattgacc atgttgtaag tgaccaaaat ccagccacct tcggcaggcg tcaatgccaa
      181 aaagccttgt aattgtggca acacggcggt gagcaaacca ttttgtaggc cgcctgtgag
      241 ggctagcacg atgccgacca aagcgtaaaa cactttgcgt cgatggggat ggtctggcac
      301 cccaggcgag cctatcatgg cgggttgttc gtgactttta aacgtgtaag tctcgggtgg
      361 catttgccac aatttcttga ttttcatgtt gtgcgatatt aattgagtgc cactgcacgg
      421 gcggttgctg ccagcgcatg gcaagatgct taaacacatt gccgctgtga aaacccgaag
      481 caggcagctg acaaaacatg ccacggatgg gcggtgagtc agcgtatttt cagacagaaa
      541 caatcgaaat cgattctact ccttttttta ttttggcgct aaacgcccac aaaaaagcgg
      601 cctgtaagtt tacaggccgc ttgtaactgc ttgattgggt ttaattcgct accgtcacac
      661 ccgctggtgc gcgcaaccaa tgctgcttga tttgctgcac cgcgcgcacc cacaccacat
      721 aacgccccgc gtcttgccaa tcggcatcgg ccacgtccac gccctcgggc aaaatctgtt
      781 gtcgaaagtc agccaagacc gcatccaatt gcttagacac attcaccgaa gtcggccagc
      841 cctgctcatc gcgcaaagcg cggttgtgca gcaacagctg tctgaccgca ggcaaaggca
      901 tttgctcgac caactcggtc aaattgctgg cggtaaacat tatgggctcg gcttgattgt
      961 ccaaatacaa gccgcattgc ccttgctcaa aacgcaggta ttcacacact tgcgccaaca
     1021 gcaattgctg ccgtatcaaa tcgcggttgc gcacattgtt ggcggccatc atgacgcgca
     1081 atgacgccag ttcgccatcc acatgccaat accacagggt tttgtcgtct ttcatggtga
     1141 gtcggtaagc cgccatcatc gccgcatagc cgtgccactc ttgcaaataa cacgtgctac
     1201 ttaaaacaat ggatatgcgg tcaacgcaag gtgaggccaa acccgacaca tgcgcttgtt
     1261 gccgcttcac ggtgtcggta atcaaaccga tgatggcatg gtcttgttgc atggattgcg
     1321 gatacaaaac cgtcacatac tcatgttggc ggctgaattc ggtcaacacc ttgcccaaag
     1381 gcttgatgtt gcggtggtac aagcggccca aggtatcggc ggcgtgttgg ctcaattgtt
     1441 gccattgcac ggggcttaat ttggccacgc gctcggtaaa tgcggcctgt aaaaattcgg
     1501 gggtgcagtg gttttgcacc aattggcggc tgcaatcttg ggcgatgcgc gcaggcaggt
     1561 tttgccactg gccttcttgc agccacaatt gcaattgggc aaaagtggcg aaattgccca
     1621 agcggcgcgt ccacgtgctc atgatttcca ccagccatgt cgccgcctgc cctttccacg
     1681 cctgcatcaa ggtgtgcaac atgtttttgt cgggctgca
//
LOCUS       DAOMVK010000501         1713 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_20474_length_1713_cov_2.021110, whole genome shotgun sequence.
ACCESSION   DAOMVK010000501 DAOMVK010000000
VERSION     DAOMVK010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20474_length_1713_cov_2.021110"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..916)
                     /locus_tag="PK856_12115"
     CDS             complement(176..916)
                     /locus_tag="PK856_12115"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRL03156.1"
                     /translation="MINHNNLEEYSDPINYDLEFGGETSKYHFYLELARLNPGEVLEL
                     ACGTGLTTLHLSKAGIPITGVDIATGMLQYAKIKAQGLPVEFIEADARTFQAEKTFAM
                     VYLTGNAFQAFLSDEDQAALLQTVCKHLAPNGLFVFETRNPAGCDLSNQEESHWGEFI
                     DKDGVTVKVSGTQSYDASKQIMHWVTFRDWGYKETTSRIACRFTDNDALKQLLSRHGL
                     RIENQYSDWNQTLFTPFASSIISVCRKA"
     gene            complement(1162..>1494)
                     /locus_tag="PK856_12120"
     CDS             complement(1162..>1494)
                     /locus_tag="PK856_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRL03157.1"
                     /translation="DYISDALAAQVGGIGIAPGANISDEYAVFEATHGTAPKYAGQDK
                     VNPGSLILSAEMMLRHLGWTEAADLVIASMEKAIGDKQVTYDFARLMEGANEISCSAF
                     GDAMIARM"
     assembly_gap    1495..1504
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1505..>1713)
                     /locus_tag="PK856_12125"
     CDS             complement(<1505..>1713)
                     /locus_tag="PK856_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRL03158.1"
                     /translation="TEGGFRDWSYALAKNEFGATEIGAGPWCEFKNPKTGKTITVKDS
                     IADAFLQQILLRPAEYDVIATLNLN"
BASE COUNT          450 a          429 c          368 g          456 t
ORIGIN      
        1 gtgagcaaca tcatggccta gggcttgaga agtgatttaa aaaatgatca gtaatgatgg
       61 gttttaaaac caaggtgttc atggtttggc tactcaatca acaccaatac ccacgtccat
      121 ccaagcacac agcccacaac atgactgggc tggacaagca gcgctaaaat cgcgctcatg
      181 cttttcggca aacactgata atggatgagg caaaaggcgt aaacagtgtt tgattccagt
      241 ctgaatattg attttcaatg cgcaaaccat gacggcttaa caattgtttc agcgcatcat
      301 tgtcagtaaa acggcaagct atgcgtgagg tggtttcctt gtatccccaa tcacgaaaag
      361 taacccagtg catgatttgc ttacttgcat catacgattg cgtgccagac accttgactg
      421 taacaccatc tttatcaatg aattcacccc aatgggattc ctcttggttt gataaatcgc
      481 atcctgcggg attgcgggtt tcgaaaacaa acaagccatt gggcgctaaa tgtttgcaga
      541 ctgtttggag caatgcggct tggtcttcat cgctcaaaaa tgcttgaaag gcattccctg
      601 ttaaataaac catcgcaaag gttttttcag cttgaaatgt gcgcgcatct gcttcaataa
      661 attcaaccgg caaaccttgg gccttgattt tcgcatactg gagcatgcct gttgcgatat
      721 cgacacctgt gataggaatg cctgctttag acaaatgcag cgttgttaaa cccgttccac
      781 acgccagttc taacacctcg cctgggttca agcgagccag ctcaagatag aaatggtatt
      841 ttgacgtttc gccgccaaat tcaaggtcgt aattgattgg gtctgaatac tcttcaagat
      901 tattgtggtt gatcaatctg tccacccctt catggccatg tgaatttcat tcaaaactgt
      961 gttgcacgca attgccatcg tcctcaactc tgcaattctt catgttaaga aagtcaccca
     1021 atcatgtttt attgcaaaag cggcctgtaa tcatgaatga cttacctgta tcgcacctat
     1081 ccattgctta aatggcaaca aacccaagcc ttgcatgcag aaaacccaag cctgagcttg
     1141 ggtttgtcaa acaaaaaccg cttacatgcg cgctatcatc gcgtcgccaa aggccgagca
     1201 actgatttca ttcgcgcctt ccatcaagcg ggcaaaatcg taagtcactt gcttgtcgcc
     1261 tatcgctttt tccatggagg caatcaccaa atcagccgct tctgtccagc ccaagtggcg
     1321 caacatcatt tctgccgaca aaatcaacga gccaggattc actttgtctt gacctgcgta
     1381 tttcggcgcc gtgccatgcg tggcttcaaa cactgcgtat tcatcggaaa tgttcgcgcc
     1441 tggggcaatg cctatgccgc ccacttgcgc tgccaaagcg tcggaaatgt agtcnnnnnn
     1501 nnnncgttca aattcaaggt ggcaatcaca tcgtactcag ctgggcgcaa taagatttgc
     1561 tgcaagaagg catcggcaat cgaatctttg acggtgatgg ttttgcccgt tttcgggttt
     1621 ttgaattcac accatgggcc tgcgccaatt tccgtggcgc caaattcgtt tttcgccaag
     1681 gcatacgacc aatcacggaa gccaccttcg gta
//
LOCUS       DAOMVK010000502         1709 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_20537_length_1709_cov_3.610036, whole genome shotgun sequence.
ACCESSION   DAOMVK010000502 DAOMVK010000000
VERSION     DAOMVK010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20537_length_1709_cov_3.610036"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..137)
                     /locus_tag="PK856_12130"
     CDS             complement(<1..137)
                     /locus_tag="PK856_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butanediol dehydrogenase"
                     /protein_id="HRL03159.1"
                     /translation="MKAARFYDRGDIRIEDIPEPVVTPGTVGVQVAWCGICGTDLHEF
                     M"
     gene            complement(511..1041)
                     /locus_tag="PK856_12135"
     CDS             complement(511..1041)
                     /locus_tag="PK856_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03160.1"
                     /translation="MRGLTSALPVYVVLADLWYGLLSNITQSLDEPSRHTPNLLPTVP
                     DVAFNWLQVLANSGMVLVMTFVLWQLLQLNKAVARKQVFNMGAFRLLGLVLTLAFSLP
                     ALYGLIMSLVHLATGHVTFSLANLRYFISALCIAWCALLCVRRLFAWWRMRKTLVIAT
                     PEKNVDPELVREHESN"
     gene            1190..>1709
                     /locus_tag="PK856_12140"
     CDS             1190..>1709
                     /locus_tag="PK856_12140"
                     /inference="COORDINATES: protein motif:HMM:NF037008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRL03161.1"
                     /translation="MSQADSLTIEKILKVAEEHFAVNGYYATSMRQLTREADVNVAAV
                     HYHFGNKEALFLAVLKRRIAPFVNMVLDNMKQKAQLHASVRPEDLVQAFVDVCLQYVR
                     DFDREAGLVTRLVSRLMVDEYKVFREQLAQEYSDVVENIFQLFEQALPHLDKETVRWR
                     IHIALSTLFNSFA"
BASE COUNT          463 a          371 c          433 g          442 t
ORIGIN      
        1 tccataaatt catgcaaatc ggtgccgcaa atgccacacc aagccacttg cacgcccacg
       61 gtgcctgggg taaccactgg ctcagggatg tcttcaatgc ggatgtcgcc acggtcgtaa
      121 aaacgtgctg ctttcatgtt tgctcctgtt taaagtgaag gctgaggtca tgtagatacg
      181 ataacagacc atgcaatggt gggtttcaat ctaagtcaac aatgcttgca atggcattgc
      241 attctgtctg attcaggcaa atgccattgt tgactgtcat gaagtaaggg gaagggtatt
      301 aaagccacat taataatggt ttgtgatgga tgaggtgaat gtagtcattt taggctgcag
      361 ttttgtggca atggctggga gtactttagg cattcttggt atgtgcaatg attgtggtaa
      421 tgttgccatt caatacaatg cggcctgtaa atcatttaca ggccgcattt tatgtatgaa
      481 atcgctttaa acgagaaatc tgtgaatacc ttaattggat tcgtgttctc gcaccagttc
      541 agggtcgaca tttttttcag gcgtggcaat gaccaaggtt ttgcgcatgc gccaccaagc
      601 aaacagacgg cgcacacaca ataaggcgca ccaagcaatg cacaaagcgc tgatgaaata
      661 gcgcaggttg gccaaagaga aggtgacatg tccagttgcc aaatgcacca ggctcatgat
      721 gaggccatac agggctggca agctgaatgc caaggtcaat accaagccca aaaggcgaaa
      781 cgcacccatg ttaaatacct gtttgcgtgc caccgctttg ttcaattgca gcaattgcca
      841 caatacaaaa gtcatgacca agaccatgcc agagttggct aaaacctgta accagttaaa
      901 cgccacatcg ggaacggtgg gaagcagatt gggcgtgtgg cggctgggct catccaaact
      961 ttgtgtgatg tttgagagca agccatacca taagtcggcc aagaccacat acacaggcag
     1021 ggcagaagtg agtccgcgca cggtgttcat tccaatgaaa acaaaggctg acagtttaaa
     1081 cgccctgttg ctttttggat agcttgcacc gttttatttg tgtaaagagc attgacatgt
     1141 aggtgtttaa aacgtatgtt tcaaacactt gtttgaaatt ctaaatgcca tgagccaagc
     1201 cgacagcctc accatagaaa aaatactcaa agttgccgaa gagcacttcg ccgtcaatgg
     1261 ctattacgcg acttccatgc gtcaactcac ccgtgaggcc gatgtgaatg tggctgcggt
     1321 gcattaccat tttggcaata aagaggcttt gtttttggct gtgctcaagc gccgcattgc
     1381 cccttttgtg aacatggtgc tcgacaacat gaaacaaaaa gcacaactgc atgccagtgt
     1441 gcggcctgaa gacttggtgc aggcctttgt ggatgtgtgt ttgcagtatg tgcgtgattt
     1501 tgaccgtgaa gcaggcttgg tgacacgctt ggtgtcgcgt ttgatggtag atgaatacaa
     1561 ggtgttccgt gaacaattgg cacaggaata ctctgatgtg gttgagaaca tcttccaatt
     1621 gtttgaacag gctttgccgc atttggacaa agaaaccgta cgctggcgca tacacattgc
     1681 actgagtacc ttgttcaaca gttttgctg
//
LOCUS       DAOMVK010000503         1703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_20631_length_1703_cov_3.763956, whole genome shotgun sequence.
ACCESSION   DAOMVK010000503 DAOMVK010000000
VERSION     DAOMVK010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20631_length_1703_cov_3.763956"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PK856_12145"
     CDS             <1..287
                     /locus_tag="PK856_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HRL03162.1"
                     /translation="DRVFRIKTERSSATMPDTRTCHNIANVEIMATEGNQATVRFNWN
                     TLSFRYKTNYSYFGMSRYVIDFSGETPQILSKYVVLKNDYINQVIDVYHI"
     gene            373..1389
                     /gene="benC"
                     /locus_tag="PK856_12150"
     CDS             373..1389
                     /gene="benC"
                     /locus_tag="PK856_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate 1,2-dioxygenase electron transfer
                     component BenC"
                     /protein_id="HRL03163.1"
                     /translation="MSNHKVALQFEDGVTRFITVANNEVLSDAAYRQKINIPMDCREG
                     ACGTCRAFCETGSYDMPEDNYIEDALTPEEAEQGYVLACQCRVTSDAVFQILASSEVC
                     KTSIHKYQGTLTDVQQLSDSTITFDIELDEDQPDIHFLAGQYVNVGIPGTEEHRSYSF
                     SSEPGNRRTSFVVRNVPEGKMSGYLSDLAKVGDKMTFTGPNGSFYLRPIKRPVVMLAG
                     GTGIAPFLSMLQVLEKEGSEQPVRLVFGVTRDCDLVSMQTLQTLQDTLPWFEYRTVVV
                     DADSSHERKGYVTQHIEADWLNGGDVDVYLCGPVPMVEAVRGWLDSEGVKPANFLFEK
                     FSAN"
     gene            1405..>1703
                     /locus_tag="PK856_12155"
     CDS             1405..>1703
                     /locus_tag="PK856_12155"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRL03164.1"
                     /translation="MQTQRFLNKVVVVTGAAQGIGEGVALQAAREGAQLVLVDRSELL
                     SDVLTAVQALGAEAIMVLADLETFAGAESAMAAAKERFGRIDVLINNVGGAIWMKP"
BASE COUNT          437 a          422 c          454 g          390 t
ORIGIN      
        1 aagaccgagt gtttcgcatc aaaaccgagc gttcttcggc caccatgccc gacacacgca
       61 cttgtcacaa cattgccaat gtggaaatca tggcaacaga aggtaaccaa gccacggtgc
      121 gtttcaattg gaataccttg agctttcgct acaaaaccaa ttacagctat ttcggcatgt
      181 cgcgctacgt catcgatttt tcgggtgaga caccgcagat tctaagcaag tacgtggtgt
      241 tgaaaaacga ttacatcaac caagtgatag acgtgtatca catataaaaa taagcacagc
      301 tggcggtaaa tctccgcgtg agccgccagc atgatgcgtt ggaacacaaa ccagagagaa
      361 gaggactgca tcatgtctaa ccataaagtc gccttacaat ttgaagacgg tgtgacccgt
      421 ttcatcaccg tcgccaacaa tgaagtcttg tctgacgccg cttaccgtca aaaaatcaac
      481 atacccatgg attgtcgcga aggcgcatgt ggcacttgcc gcgcgttttg cgaaacaggc
      541 agctacgaca tgcctgaaga caattacatc gaagacgcct taacccctga agaagccgaa
      601 caaggctatg tgttggcttg ccaatgccgc gtgacatccg atgcggtatt ccaaatcttg
      661 gcttcttccg aagtgtgcaa aaccagcatc cacaaatacc aaggcacttt aaccgatgtg
      721 caacaattgt cggattctac catcacgttt gacatcgaat tagacgaaga ccagcccgac
      781 attcatttct tggcaggcca atacgtcaac gtgggcatac caggcacaga agaacaccgc
      841 tcgtattcat tcagctcaga gccaggcaac cgccgcacca gttttgtggt gcgcaacgtg
      901 cccgaaggca agatgagtgg ctatttgagc gacttggcca aagttggcga caaaatgacg
      961 ttcacaggcc cgaatggcag cttttattta cgtcccatca agcggcctgt agtgatgttg
     1021 gcgggcggta cgggcattgc accgttctta tccatgttac aagtgttgga aaaagaaggc
     1081 tctgagcagc cagtgcgctt ggtgtttggt gtgacccgtg attgcgattt ggtgtccatg
     1141 caaaccttgc aaaccttgca ggataccttg ccttggtttg aataccgcac tgtggtggtc
     1201 gatgccgaca gcagccacga gcgcaaaggc tatgtcaccc agcacatcga agccgattgg
     1261 ctcaatggcg gcgacgttga tgtgtatctg tgcggccctg tgcccatggt ggaagcggtg
     1321 cgcggttggt tggacagcga aggcgtgaaa cctgccaatt tcttgtttga gaaattctct
     1381 gccaattaaa ccaaggacac aaccatgcaa acgcaacgat ttctcaacaa agtggtggtg
     1441 gtcactggcg ctgcccaagg cataggcgag ggcgtggctt tacaggccgc tcgtgagggc
     1501 gcgcaactgg tcttggtaga ccgttctgaa ttgcttagcg atgtattgac cgcagtgcag
     1561 gcattggggg cagaagccat tatggtgttg gcggatttgg aaacttttgc tggcgcagag
     1621 tcggccatgg cggcggcaaa agagcgtttc ggtcgcatcg atgtgttgat taacaatgtc
     1681 ggtggcgcga tttggatgaa gcc
//
LOCUS       DAOMVK010000504         1688 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_20855_length_1688_cov_2.182486, whole genome shotgun sequence.
ACCESSION   DAOMVK010000504 DAOMVK010000000
VERSION     DAOMVK010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20855_length_1688_cov_2.182486"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PK856_12160"
     CDS             complement(<1..374)
                     /locus_tag="PK856_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRL03165.1"
                     /translation="MISDNHPFKRIQQIMLDPIDKWMILAMLIIYVMSLFLLYSADGQ
                     EFVQLEKKTLHTFIGFLLLWGLARIKPQILASLALPAYIGGVVLLVAVELFGVTVNGS
                     TRWLDIGPVRIQPSEIMKIGLP"
     gene            complement(371..>754)
                     /locus_tag="PK856_12165"
     CDS             complement(371..>754)
                     /locus_tag="PK856_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003787661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRL03166.1"
                     /translation="GKTGTAQVKSIAQGKSYNAAALAVQHRDHAWFISFAPVDKPKIA
                     IAVILENGGWGATAAPVARQLTDFYLLKILGQGSSNELSTPQGMTVLADETLISESSK
                     TSLASLQAAFAAARQAPQAASGVKP"
     assembly_gap    755..764
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<765..>1688)
                     /locus_tag="PK856_12170"
     CDS             complement(<765..>1688)
                     /locus_tag="PK856_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRL03167.1"
                     /translation="LNNTPAQSGQTLRLAMDIRMQQKADELLGNRRGAVVAIDPQTGG
                     VLALVSKPSFDPNLFIDGIDSENWKALNENWETPMINRTIQGLYPPGSTFKPFISMAI
                     LESGKLTQTSVVPAPGAWSIPGSSHQYRDSVRSGHGAVNLQRAITVSSDTFFYRIGYE
                     MGIDKVAPYLKQFDLGQLTGIDLPNESKGILPSREWKKERFKKSPKNQEWHPSEMVSI
                     SIGQGYNTYTPLQMAHATAILANNGVVYRPHVVKELHNHQTQQITLVDPKPERTLPYK
                     QSNFDYVKKGMEGVLRPGGTGWRVGSGLQYT"
BASE COUNT          406 a          390 c          439 g          443 t
ORIGIN      
        1 atcggtaagc caattttcat gatttctgag ggctgaatgc ggacagggcc gatgtctaac
       61 caacgggtag aaccgttgac ggtaacacca aacaattcca ctgccaccaa taaaaccacg
      121 ccgccgatat aagcaggtaa ggccaaactg gccaagattt gcggtttgat gcgcgccaaa
      181 ccccacagca acaaaaagcc gatgaaggtg tggagggttt ttttctccaa ttgcacaaac
      241 tcttggccat cggcagagta gagcaagaac aggctcatca cataaatgat gagcatggcc
      301 aaaatcatcc atttatcaat ggggtcgagc atgatttgct ggatgcgttt aaatgggtgg
      361 ttgtctgaaa tcatggtttc acacctgatg ctgcttgtgg ggcttggcgt gcggcagcga
      421 aagcggcctg taaagatgcc agcgaggttt tgctgctttc cgaaatcaag gtttcatctg
      481 ccaacactgt catgccttgc ggcgtgctta gctcattgct tgaaccttgc cccaaaattt
      541 tcaatagata aaagtcggtc aattggcgag caacaggcgc tgcggttgca ccccaaccgc
      601 cgttctccaa aatcactgca atggcgattt tgggtttgtc cactggtgca aacgaaatga
      661 accaagcgtg gtcacggtgt tgcacagcca aagccgcggc attgtaagac ttgccttggg
      721 cgatgctttt cacctgtgcc gtaccggttt taccnnnnnn nnnnatggtg tattgcaagc
      781 cagagcccac gcgccaaccc gtaccgcctg ggcgcaacac gccttccata ccttttttaa
      841 catagtcaaa attgctttgt ttataaggca aagtgcgttc aggcttgggg tcgaccaagg
      901 tgatttgttg ggtttggtga ttgtgcaatt ctttcaccac atgcggcctg taaaccacac
      961 cattgtttgc taagatggct gtggcatgtg ccatttgcaa aggggtatag gtgttatagc
     1021 cttggcctat gctgatggaa accatttcag atgggtgcca ttcttggttt ttaggcgatt
     1081 ttttgaaacg ctctttcttc cattcacgtg aaggcaaaat gcctttgctt tcattcggta
     1141 agtctatgcc tgtcaattgg cctaagtcga actgtttcaa ataaggagca accttatcga
     1201 tgcccatttc ataaccaatg cggtagaaaa aagtgtctga ggaaacagta atggcccgtt
     1261 gcaagttcac tgcgccatgg cctgagcgta ctgagtcacg gtattggtgt gacgagccag
     1321 gaatgctcca agcgccaggg gcgggaacca cgctggtttg tgtcagcttg ccactttcga
     1381 gtatggccat ggatatgaat ggcttgaagg tagaaccagg agggtagagg ccttgtatgg
     1441 tgcggttaat cattggggtt tcccaatttt cattcaaggc cttccagttt tcactgtcta
     1501 tgccatcgat aaacagattt gggtcaaatg aaggtttgga taccaaggcc aatacgccac
     1561 ctgtttgagg gtctatcgcc accactgcgc cgcggcgatt gccgagcaat tcatcggctt
     1621 tttgctgcat gcggatgtcc atggccaagc gcaaggtttg gccagattgg gcaggggtgt
     1681 tgtttaaa
//
LOCUS       DAOMVK010000505         1683 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_20939_length_1683_cov_2.865479, whole genome shotgun sequence.
ACCESSION   DAOMVK010000505 DAOMVK010000000
VERSION     DAOMVK010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20939_length_1683_cov_2.865479"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1274
                     /locus_tag="PK856_12175"
     CDS             <1..1274
                     /locus_tag="PK856_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRL03168.1"
                     /translation="VAMCAQVFGAVIDNGKMLRNFVQIMRSGAVGRKSLGTRPKKLVQ
                     NWLLNASEAQLLSASVGNTPSLADVVKMVHPKPDEAWRAAWFAWLIGREYTFADLPAL
                     TQEFEHFKRNTSRLVPNVPFQLLTALDLSKASWRQIAKQGSWQMVRQNLNTFMRHGVV
                     VAGKDGKEANMIASKLKDKETIAKARVLPYQIMTSLQAVNTGMPKVITDALETALEIS
                     LMNVPRLEGNVHVCMDVSGSMTSPASGYREGATSVTTCLDVAALIAAAVVARNPHANV
                     LPFDDMVRLNHRVSANNSVLKNAQILQKMLGGATDCAAPLRYINEKALPVDLVVFVSD
                     NQSWVNNQAYYAGTTKMQEWEKIKARNPRARMVCIDIQPYTTVQAEPREDVLHVGGFS
                     DAVFTLLNQYARGELGSEHWVGVINAVPLPN"
     gene            1297..1491
                     /locus_tag="PK856_12180"
     CDS             1297..1491
                     /locus_tag="PK856_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03169.1"
                     /translation="MELHPLGGRYTGSTPVATAECLQDHTNIQWWRSLTVERVTRFIT
                     PRRQTLNQRPVIHKTGLQAA"
BASE COUNT          432 a          413 c          455 g          383 t
ORIGIN      
        1 acgtggccat gtgtgcccaa gtgtttggtg cggtcatcga taacggtaag atgttacgca
       61 actttgtgca aatcatgcgc agcggtgctg tgggtcgcaa atcattggga acgcgtccga
      121 aaaaactggt acaaaactgg ttgttgaacg ccagcgaagc acaattgctg tctgccagcg
      181 tgggcaacac gccatcgctt gccgatgtgg tgaaaatggt gcacccaaaa cctgatgaag
      241 cgtggcgcgc cgcgtggttt gcatggttaa ttggtcgtga atacacattc gccgatttac
      301 cagccttaac ccaagaattt gaacacttca aacgcaatac aagccgcttg gtgccgaatg
      361 tgccgttcca attgttgacc gccctggatt tgagcaaagc ctcatggcga caaattgcca
      421 agcaaggttc atggcaaatg gtgcgtcaaa acctgaacac gtttatgcgc cacggcgtgg
      481 tggtggcagg aaaagatgga aaagaagcca acatgattgc ctccaaattg aaggataaag
      541 aaaccattgc caaagcgcgc gtgctgcctt accaaatcat gaccagcttg caagcggtga
      601 acacgggcat gcccaaagtc atcaccgatg cattggaaac tgctttggaa atctcgctga
      661 tgaacgtacc acgcctagaa ggcaatgttc atgtgtgcat ggatgtgtcg ggttcgatga
      721 cttctcctgc aagcggttac cgtgagggag cgacttcggt caccacgtgt ttggacgtcg
      781 ccgccttgat tgccgccgct gtggtagcgc gcaatccgca tgcaaacgtg ttgccgtttg
      841 atgacatggt gcgcttgaat caccgcgtca gcgccaacaa cagcgtgttg aaaaacgcgc
      901 agattttgca aaaaatgctc ggtggagcca ccgattgcgc cgcacctttg cgctacatca
      961 atgaaaaagc cttgccggtg gatttggtgg tgtttgtgtc ggacaaccag tcttgggtga
     1021 acaaccaagc gtattacgca ggaacgacca agatgcaaga atgggaaaaa atcaaagcgc
     1081 gcaatccccg tgcccgcatg gtgtgtattg atattcaacc ttacaccacc gtgcaagcag
     1141 agccacgtga agatgtgttg cacgtgggtg ggttcagcga tgccgtgttt accttgttaa
     1201 accagtatgc ccgcggtgaa ttggggagcg agcattgggt gggcgtgatc aacgccgtgc
     1261 ctttgcccaa ctaaaaacct tttttggcga atgccgatgg aactacatcc cttaggagga
     1321 cgatatacgg gttcgactcc cgtcgccact gctgagtgtt tgcaagatca tacgaacatt
     1381 caatggtggc gtagcttaac ggtagagcgc gtaacacgtt tcatcacccc tcgtcgccaa
     1441 acccttaacc agcggcctgt aattcacaaa acaggtttac aggccgctta atgcaacaag
     1501 cagtaacaca agtttcgcaa cgaatgccga tgggactaca ggcattccga gtgctgcaca
     1561 tggggcgaaa tcccatgttc agaatcggga attaaaagac gtctcatcac tttttgtcgt
     1621 tgcacccttt tttggcgaat gccgatggaa ctacattctt agggagaacg agatgcgggt
     1681 gcg
//
LOCUS       DAOMVK010000506         1671 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_21153_length_1671_cov_1.640470, whole genome shotgun sequence.
ACCESSION   DAOMVK010000506 DAOMVK010000000
VERSION     DAOMVK010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21153_length_1671_cov_1.640470"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..510
                     /locus_tag="PK856_12185"
     CDS             202..510
                     /locus_tag="PK856_12185"
                     /inference="COORDINATES: protein motif:HMM:NF025198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HRL03170.1"
                     /translation="MLPENQSPPSRSQPYPKEDQTNVLAVVSLISSLLSWVLIPVIGA
                     IVGVVCGHIARGQLRYQPNESSSIMALLGLIIGYVQLVFMVVVLVAIGFFGWVIFQNA
                     "
     gene            662..1366
                     /locus_tag="PK856_12190"
     CDS             662..1366
                     /locus_tag="PK856_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03171.1"
                     /translation="MNRRQFCLGSVVLTAVAVSGCATRSVWDDVSSTKYHTYYDQINA
                     FYVNPDQSQFVILSDRFHYIFNTRSVIPLLKSSLRSRYIVEIQNMRMPEPSAANELPI
                     TGRLVFALPVSKLSAGDLKQAKEMGFVSASTTEKQKALEVAKLRNIDVDEGSEYLIQT
                     VSIAGRRYSPKAGVSYGNSQHFYRQYNVPVVQERTSVNASKAIGATLVTPITLAADGV
                     LLLLGLPLLAVFVMGN"
     gene            complement(1401..>1671)
                     /locus_tag="PK856_12195"
     CDS             complement(1401..>1671)
                     /locus_tag="PK856_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03172.1"
                     /translation="TAVRPLSMPAKEAIMGKRLNDNRVNNTQKYTWLFKRRFTADTFI
                     YIFFTLINDIIHENTAQIEFDCRHVLNISQSITWLYARPDTDKDG"
BASE COUNT          400 a          324 c          454 g          493 t
ORIGIN      
        1 tgtatcatgg ccaagacatc gcttttgttg gtaaaaaatg agggtgaaat caaggtcaca
       61 tcacacatac tgactccttt acacaatagg catggtttca tactaaacgg tttaaaatga
      121 actgtgtaaa aattaagcag aatctttgtg atgtgtcggg tttcgtgtgg gatttatacc
      181 gtttttgaag ggagttgtac catgttgcca gaaaatcaaa gtccgccatc gcgttcgcag
      241 ccttatccaa aagaggatca aaccaatgtg ttggcggtgg tcagcttgat cagcagcctg
      301 ttatcttggg tgttgattcc cgtgattggt gcgattgtgg gggtggtgtg tgggcacatt
      361 gccagaggcc aattgcgcta tcagcccaat gaatcgagca gcatcatggc tttgttgggc
      421 ttgatcatcg gttatgtgca attggtgttc atggtggtgg tgttggttgc gattggtttt
      481 tttggttggg tcatttttca aaatgcctga agccattggc tttttttgtg gcaccaaaca
      541 ataccgtaag cgccaaattt ctgcacttga acggcaaaat agatggcgct tgagcaattg
      601 acttaatatg cacttggcaa tcgtgccttg aatgagactg cataacacag gggagcttgc
      661 gatgaacaga cgtcaatttt gcttgggttc ggttgtgctg acggccgttg ctgtcagtgg
      721 ctgtgccacc cgttcggttt gggatgatgt gtccagcacc aaataccata cctattacga
      781 tcagatcaat gcgttttatg tgaatccaga ccaaagccaa tttgtgattt tgtctgatcg
      841 ctttcattat attttcaata cccgcagtgt gattccgttg ctgaagtcaa gtttgcgcag
      901 tcgctatatt gtggaaattc aaaacatgcg catgccagag cccagtgctg ccaatgagtt
      961 gccgattaca ggccgcttgg tgtttgcatt gccagtgagt aaattgagcg cgggtgattt
     1021 aaaacaagcc aaagagatgg gttttgtaag cgcgtcaacc actgaaaagc aaaaagcact
     1081 ggaagtggcg aaactgcgca atattgatgt ggatgaaggc agtgagtacc tgatacaaac
     1141 cgtgagcatt gcggggcgac gctacagtcc taaagcaggt gtgagctatg gcaacagcca
     1201 gcatttctac cgccaataca atgtgcctgt ggtgcaagag cgcaccagtg tcaatgcctc
     1261 caaagccata ggtgccactt tggtgacgcc gattaccttg gcggccgatg gggtgttgtt
     1321 gctattgggc ctgcctttgt tggctgtgtt tgtgatgggg aattgagtgt ggggtgggtg
     1381 gtgcagcggt aatggctgtg tcatccatct ttatcggtgt caggacgggc ataaagccat
     1441 gtgattgatt ggcttatgtt gagaacatgg cggcaatcaa attcgatttg ggctgtgttt
     1501 tcgtgtatta tgtcgttaat taatgtaaaa aagatgtaaa tgaaggtgtc agcagtaaat
     1561 cgtcgtttaa acagccatgt gtatttttgt gtgttgttta ccctattgtc attcagtctc
     1621 ttgcccatga tcgcctcttt agcaggcata ctcagtgggc gcactgccgt g
//
LOCUS       DAOMVK010000507         1667 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_21215_length_1667_cov_2.758065, whole genome shotgun sequence.
ACCESSION   DAOMVK010000507 DAOMVK010000000
VERSION     DAOMVK010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21215_length_1667_cov_2.758065"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1667)
                     /locus_tag="PK856_12200"
     CDS             complement(<1..>1667)
                     /locus_tag="PK856_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017507098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRL03173.1"
                     /translation="HGKSMVIVGAGLNHWYHMDMSYRGIINMLMMCGCVGQSGGGWAH
                     YVGQEKLRPQTGWAPLAFALDWQRPARQMNGTSFFYAHTSQWRHEKLGMDEVLAPTQG
                     HELENISMIDYNAKSERMGWLPSAPQLSVNPLKITQDAKEQGQNAVAYTVSGLKDGSI
                     DMACNDPDNPANFPRNLFVWRSNILGSSGKGHEYFLKYLLGTQNALMSNEEDCIKPQE
                     ITVRPAVEGKLDLLTVLDFRMSTTCLYADIVLPTATWYEKDDLNTSDMHPFIHPLSEA
                     VNPLWESKSDWEIYKGLAKKFSELAKDYLGVQQDIVLTPLMHDSPQELGQAFDAKDWK
                     KGECEPIPGKTMPAMTVVERDYAAIYDKYTAIGPLLEKLGNGGKGINWKTEHEVDILR
                     GINKVQASGVGKGQPKLNTAIDAAEMILTLAPETNGHVAVKAWDALSQNTGIDHTHLA
                     LPREHDSIRFRDVQAQPRKIISSPTWSGLESEEVSYNAGYTNVHELIPWRTITGRQQF
                     YQDHQWMRAFGEGFCVYKPAVDLKTTAKVLGKHPNGEKEIVLNFLTP"
BASE COUNT          364 a          414 c          420 g          469 t
ORIGIN      
        1 cggcgtgagg aagttgagca cgatttcttt ctcgccattt gggtgtttgc ccaatacttt
       61 ggcagtcgtt ttcaggtcta ccgcaggttt gtacacacag aagccttcac caaaagcccg
      121 catccattga tggtcttggt agaactgttg gcggcctgta atcgtgcgcc aagggattaa
      181 ttcgtgcaca ttggtgtagc ctgcgttgta gctgacttct tcactttcca agccagacca
      241 agtcggtgag gaaatgattt tgcgaggttg ggcttgcaca tcgcggaaac ggatggaatc
      301 gtgttcgcgt ggcaaggcta agtgggtgtg gtctatgccc gtgttttgag acaaggcatc
      361 ccatgctttc actgccacat ggccgttggt ttcgggagcc aaagtcaaaa tcatttcggc
      421 tgcatcaatg gcggtattga gcttgggttg gcctttgccg acgccacttg cttgtacctt
      481 gttgatgcca cgtaaaatgt caacttcgtg ttcggttttc cagttgatgc ctttgccgcc
      541 attgcccaat ttttccagca aggggccaat tgccgtgtat ttgtcgtaaa tcgccgcgta
      601 atcacgctcg accaccgtca ttgctggcat ggtcttgcca ggaatcggtt cgcactcacc
      661 ttttttccag tctttggcat caaaggcttg tcccaattct tgcgggctgt catgcatcaa
      721 aggggttaac acgatgtctt gctgtacgcc caaatagtct ttggccaatt cagaaaattt
      781 cttcgccaag cctttgtaaa tttcccagtc agatttgctt tcccacagcg gattcactgc
      841 ctcgctcaat gggtgtatga atgggtgcat gtctgaggtg ttcaaatcgt ctttttcata
      901 ccaagtggca gtaggtaaaa cgatgtcggc atacaaacaa gtggtggaca tgcggaagtc
      961 caacacagtc aacagatcca acttgccttc caccgctgga cgtacggtaa tttcctgagg
     1021 cttgatgcaa tcttcttcat tactcatcag tgcgttttgt gtgcccaaca ggtatttcaa
     1081 gaaatactca tggcctttgc ctgaagaacc caaaatattg gaacgccaca caaacaaatt
     1141 gcgcgggaag ttggcaggat tgtctggatc gttacacgcc atgtctatgc tgccatcttt
     1201 caggccgctt accgtgtaag ccaccgcatt ttggccttgt tctttggcat cttgggtgat
     1261 ttttagcgga ttgacagaca attgtggtgc cgatggcagc cagcccatgc gctctgattt
     1321 ggcgttgtaa tcaatcatgg aaatgttttc caactcatga ccttgggtcg gtgccaaaac
     1381 ttcatccatg cccaattttt catggcgcca ttggctggtg tgggcgtaga agaacgacgt
     1441 gccattcatt tggcgggcag ggcgttgcca atccaaggca aaggccagtg gtgcccagcc
     1501 tgtttgtggg cgcagttttt cttggcccac ataatgtgcc cagccaccac ctgattgacc
     1561 gacacagcca cacatcatca acatgttgat gatgccgcgg taagacatgt ccatgtggta
     1621 ccaatgattc aaacccgcgc caacgatgac catggatttg ccgtgtg
//
LOCUS       DAOMVK010000508         1665 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_21258_length_1665_cov_1.857143, whole genome shotgun sequence.
ACCESSION   DAOMVK010000508 DAOMVK010000000
VERSION     DAOMVK010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21258_length_1665_cov_1.857143"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>226)
                     /locus_tag="PK856_12205"
     CDS             complement(<1..>226)
                     /locus_tag="PK856_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRL03174.1"
                     /translation="RLLADLPFQLTGAQQKVLAEIEQDMLQSYPMHRLLQGDVGSGKT
                     IVSALAALNAIEAGAQVAVMAPTEILAEQHL"
     assembly_gap    227..236
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<237..965)
                     /locus_tag="PK856_12210"
     CDS             complement(<237..965)
                     /locus_tag="PK856_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecG"
                     /protein_id="HRL03175.1"
                     /translation="MNLIDLGVTDKVQAKLAKLHIHNVWDAVLHLPLRYEDETHITMI
                     DDAQLGVSVQVEGEVLLQEVQFKPRKQLVVKLQDDAGSVLMLRFIHFYPSQQQQLGKG
                     KRVWAVGEIKQGYYGDEMIHPKIRAADNHALSETLTPIYPTVSGLTQPTLRKILHAAL
                     SKANLDDTLPNALLQQLHLPAFADSVRLLHDPTPEYTTQQLHDGKLPAWQRLKFDELL
                     AQQLSMKLAHEQRRSGKAPVLQGDG"
     gene            1426..>1665
                     /locus_tag="PK856_12215"
     CDS             1426..>1665
                     /locus_tag="PK856_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRL03176.1"
                     /translation="MYTSAAQHLTTLRDVFRYTVSQFNAAELFYGHGNDNAFDEAAYL
                     ILHCLQLPSDTLDTFYDAKLLPDETEAILAVVHERI"
BASE COUNT          402 a          419 c          381 g          453 t
ORIGIN      
        1 gcaggtgttg ctcggccaaa atttcggtag gtgccattac tgccacttgc gctccagcct
       61 caatcgcatt gagtgccgcc agtgccgaaa caatggtttt gccactgccc acatcgcctt
      121 gcaacaagcg gtgcatggga taagactgca acatatcctg ctcaatttcg gccaatactt
      181 tttgctgcgc gccggtcaat tgaaagggca aatccgccag caaacgnnnn nnnnnnccca
      241 tcgccttgca atacaggcgc tttgccactg cggcgctgct catgcgcgag cttcatcgac
      301 aattgttgcg ccaacaattc atcaaatttc aagcgttgcc acgcaggcag cttgccatcg
      361 tgcaattgct gggtagtgta ttctggggtg ggatcgtgca ataagcgcac gctgtcggca
      421 aatgcgggca aatgcaattg ctgtaacagc gcgtttggta aggtgtcgtc tagattggct
      481 ttggacaaag cggcatggag gattttgcgc agcgtcggct gggtcaagcc actgacggtg
      541 gggtaaatcg gcgtcaaggt ttctgacaag gcatggttgt cggcggcgcg aattttcggg
      601 tgtatcattt catcgccgta atagccttgc ttgatttcac ccacagccca cacccgcttg
      661 cctttgccca attgctgttg ttggctggga taaaaatgga tgaagcgcag catcaacaca
      721 ctgcccgcat cgtcttgcag ctttaccacc aattgtttac gcggtttgaa ttgcacctct
      781 tgcagcaaca cctcgccctc tacctgcacc gacacaccca attgggcatc gtctatcatg
      841 gtgatgtggg tttcgtcttc ataacgcagc ggcaggtgca aaaccgcatc ccaaacattg
      901 tgtatgtgca gtttcgccaa tttggcttgg actttgtcgg taacacccaa atcaattaaa
      961 ttcattgcga tgctcggccc gacaaaaatg gtggaaaggc tttcatgttc aatgcgtctc
     1021 agccgatgat aatgaagcaa gattatatga tgacacttgc ctcgatgcca gcccagcttg
     1081 ttctgacacg gtattgatac ccaaataagc ggcctgtaaa tggcggccac agaaaaagcg
     1141 ccttattgct aagacgcttt ctttccctta cttatcaact actggtattg cagtttgata
     1201 cagcatctta ttgatgcgat tgatattcaa tttttatgca atcagtatac aataattgta
     1261 tggcatattt gttgcgatac ctcaacaaac ggggtcaaat attgcataaa acccacgatg
     1321 gctatcattt aatcaaagta tctgccagca caaaactcta aatcactact tacaggccgc
     1381 ttacgttata atattggtca ttttatcgtt ttgaattggc atgccatgta cacctctgct
     1441 gcccagcatt taaccaccct ccgtgatgtt ttccgttata ccgtcagcca gttcaatgcc
     1501 gctgaattgt tttatggcca cggcaatgac aatgcgtttg acgaagccgc atatttgatt
     1561 ttgcactgct tacaattgcc ttccgacact ttggacacgt tttacgatgc caaattgttg
     1621 ccagatgaaa ccgaagccat tttggcggtg gtgcatgaac gcatc
//
LOCUS       DAOMVK010000509         1661 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_21323_length_1661_cov_3.437733, whole genome shotgun sequence.
ACCESSION   DAOMVK010000509 DAOMVK010000000
VERSION     DAOMVK010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21323_length_1661_cov_3.437733"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="PK856_12220"
     CDS             <1..504
                     /locus_tag="PK856_12220"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HRL03177.1"
                     /translation="PEGMGWSALRPILPVALSYVLSFVYVGIYWNNHHHLFQLAQKTS
                     GSVLWANLFLLFWLSLFPITTAWLGDHPHASAPSMLYGMVLLMGAISFYVLEQRIIAV
                     DPNNQALKIALGRNFKGKLSPLCYVLGIVLAYFSPLLGQLIYALMAALWLIPDRGMEQ
                     AAQSHPE"
     gene            complement(589..>1661)
                     /locus_tag="PK856_12225"
     CDS             complement(589..>1661)
                     /locus_tag="PK856_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HRL03178.1"
                     /translation="FTYAVCLGMGVPWQVALAAVFVSGLLFFALSFSNIREALINAIP
                     MTLKLAIGGGIGLFLALVGLKTSGIIVANEDTYVSLGNLQTHGPLFTLFGLLLIVIMH
                     HYKVRGAIIISILSVTALSTLLGYNQIVGVVGAVPSIAPTFMQMDFKDLFTPAMIGVI
                     FVFFLVDLFDSTGTLVGVANRAGLLQDGKLPRLKRALLADSTAITAGAVLGTSSTTPY
                     IESASGVAAGGRTGLTAIVVGVLLLACLFFAPLAKSVPAYATAPAVIYVGVLMIQGIT
                     GIKWDDITEAVPAFLTIVFMPFTYSIADGIAMGFISYALVKLCTGQAKTVPYMVWIVA
                     FVWGWMFFMPKAVVQQLLSVFA"
BASE COUNT          446 a          446 c          413 g          356 t
ORIGIN      
        1 ccagaaggca tgggttggtc ggcgttgcgt cccattttgc ccgttgccct cagctatgtg
       61 ctcagtttcg tgtatgtggg catttactgg aacaaccacc accacctgtt tcaattggcg
      121 caaaaaacct caggttcggt gttgtgggcc aatttgtttt tgttattttg gttgtcacta
      181 tttcccatca ccaccgcttg gttgggcgac cacccacatg ccagtgcgcc gagcatgttg
      241 tacggcatgg tgttgctgat gggagccatc agtttttacg tattggaaca acgcatcatc
      301 gccgtagacc ccaataacca agccctcaaa atcgcattgg ggcgcaattt caaaggtaag
      361 ttgtccccct tgtgttatgt gctgggcatc gttttggctt atttttcccc gctattaggg
      421 caattgatat acgccctcat ggcggcattg tggttgatac ccgaccgcgg catggagcaa
      481 gcggctcaat cccatcctga ataaacgccc aacgcaattt aataactaaa taagcaataa
      541 aagcggcctg taaatgtaca ggccgctttt caattactca ataagctatt aagcaaacac
      601 gctcaacaat tgttgcacca ctgctttggg catgaagaac atccagcccc acacaaaggc
      661 gacgatccac accatatacg gtacggtttt ggcttggcct gtgcacagtt tcaccaaggc
      721 gtagctgatg aagcccatgg cgatgccgtc ggcaatcgaa taggtgaacg gcatgaacac
      781 aatggtcaag aacgcaggca cggcttcggt gatgtcgtcc catttgatgc cggtgatgcc
      841 ttgtatcatc aacacgccca cgtaaatcac cgcaggggcg gtggcgtaag cgggcacact
      901 tttggccaaa ggcgcgaaga acaaacacgc gagcaacaaa acgcccacca cgatggcagt
      961 caaacctgtg cggccaccag cggccacgcc agaagccgat tcaatgtaag gcgtggtaga
     1021 cgaggtgccc aatactgcgc ctgcggtaat cgcggttgag tcggccaata aagcgcgttt
     1081 caaacgcggc aatttgccgt cttgcaacaa gcctgcgcgg ttggccacgc ccaccaacgt
     1141 gccggtagaa tcgaacaaat ccaccaagaa aaacacgaaa atcacgccta tcattgcagg
     1201 ggtgaacaag tctttaaaat ccatttgcat gaaagtaggc gcaatcgatg gcaccgcacc
     1261 caccacaccg acgatttggt tgtagcccaa taacgtcgac aatgccgtta ccgacaaaat
     1321 gctgatgatg atggcgccgc gcactttgta atggtgcatg atgacgatca gcaacaagcc
     1381 aaacaaggtg aacaaggggc cgtgggtttg caaattgccc aagctcacgt aagtgtcttc
     1441 gttggccacg atgatgcccg aggttttcag gccgaccaaa gccaagaaca aaccgatgcc
     1501 gccgcctatg gcgagtttca aagtcatggg aatggcgtta atcaaggctt cacggatgtt
     1561 ggaaaagctc aaggcaaaga ataacaaacc cgacacaaac acggcagcca aagccacttg
     1621 ccaaggcact cccatgccca aacacacggc gtaagtgaaa t
//
LOCUS       DAOMVK010000510         1657 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_21387_length_1657_cov_2.990637, whole genome shotgun sequence.
ACCESSION   DAOMVK010000510 DAOMVK010000000
VERSION     DAOMVK010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21387_length_1657_cov_2.990637"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(459..>1657)
                     /locus_tag="PK856_12230"
     CDS             complement(459..>1657)
                     /locus_tag="PK856_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HRL03179.1"
                     /translation="MIASLQDYLKAITGFDEISMQPNSGAQGEYAGIVAIRRYQEAQG
                     ESHRDICLIPRSAHGTNPATANMVGLKVVGVDTDEHGNVDIADLKAKAEQHKDNLSAL
                     MITYPSTHGVFEEGIREICSIIHANGGQVYMDGANLNAQVGLMQPAEVGADVLHMNLH
                     KTFCIPHGGGGPGMGPIGMRAHLAPFMANHVVSAVPHAQPGQGAVSAAPFGSASILPI
                     TWMYITMMGKDIKQATEAALLSANYLATKLGEDYPILYSGKNGRVAHECIVDLRPLKA
                     DSGITEVDIAKRLMDYGFHAPTMSFPVAGTLMIEPTESENKAELDRFIAAMKQIKQEA
                     LKVQNGVWPKDDNPLCNAPHTNFNVTDGEWTHPYSREEAVYPLAYVRGDRNLICSCPP
                     MDAYED"
BASE COUNT          389 a          409 c          404 g          455 t
ORIGIN      
        1 gcccatgcca gtgctggcga aaacaacagt gtgtaaccca aatatttgaa taagcgcttc
       61 atcatttccc tttgttaaaa gtgtaaacac atcccattca gcattttaga tttgggcaac
      121 ggcatcaaaa cataagccat atctgaattt tcagcgttgc ttgaaaacac agagatgatt
      181 tctgtcattt cgcccgcccc atctgtgcaa aatgcctcga caacagccca ttgttttatt
      241 tctggcctta attcgcacaa aaataaacca tatgcatata atctcaaatt tattcaaatg
      301 aatcaatgct taaaatacca aacctgcgtt tcaacaccag tctgtgcatg gccttggtct
      361 gaacttcaat ctttctgtgt ttgtgagcac atttcatgtc aaaaaagcgg cctgtaaatg
      421 cttacaggcc gcttgtttac tacacacaca tttattgctt agtcttcgta tgcatccata
      481 ggagggcatg agcaaatcaa attgcggtcg ccacgcacgt aagccaatgg gtatacggct
      541 tcttcgcggc tgtatggatg ggtccattcg ccgtcggtca cgttgaagtt ggtgtgcgga
      601 gcattgcaca aaggattgtc gtccttaggc catacgccgt tttgcacttt caaggcttct
      661 tgcttgattt gtttcattgc tgcgatgaag cggtcgagtt cggctttgtt ttccgactcg
      721 gtaggctcaa tcatcaaggt gcctgccact gggaacgaca tggttggtgc gtggaaaccg
      781 tagtccatca agcgtttggc gatgtcaact tcagtgatgc cgctgtctgc tttcaatggg
      841 cgcaagtcaa cgatacattc gtgagcgacg cgaccgtttt taccgctgta caaaattggg
      901 tagtcttcgc ccaatttggt tgccaagtag ttggcgctca acaaagccgc ttcggtggct
      961 tgcttgatgt ctttgcccat catggtgatg tacatccatg tgataggcaa aatgctggct
     1021 gagccaaatg gtgccgcaga aaccgcgcct tggcctggtt gtgcatgagg cacagcagac
     1081 acaacgtggt tggccatgaa tggggccaaa tgggcacgca tgccgatagg acccatgcct
     1141 gggccgccgc cgccatgagg gatacagaag gttttgtgca agttcatgtg taacacgtct
     1201 gcgcccactt ctgctggctg catcaagccc acttgcgcgt tcaagttcgc gccgtccatg
     1261 tacacttgac caccgtttgc atggatgatg ctgcaaatct cgcggatgcc ttcttcaaac
     1321 acaccgtgag tcgatgggta ggtaatcatc aacgcagaca agttgtcttt gtgttgttca
     1381 gctttggctt tcaaatcagc aatgtccaca ttaccgtgtt catcggtgtc cacacccact
     1441 actttcaggc cgaccatgtt ggcggtggct gggttggtac cgtgagcaga gcgaggaatc
     1501 aagcagatgt cacgatgtga ctcaccttgg gcttcttggt agcggcgaat cgccacgata
     1561 cctgcgtact caccttgcgc acctgagtta ggctgcatgg agatttcgtc gaaaccggtg
     1621 atggctttca aatagtcttg caagctggca atcattt
//
LOCUS       DAOMVK010000511         1654 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_21433_length_1654_cov_2.737961, whole genome shotgun sequence.
ACCESSION   DAOMVK010000511 DAOMVK010000000
VERSION     DAOMVK010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21433_length_1654_cov_2.737961"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="PK856_12235"
     CDS             <1..967
                     /locus_tag="PK856_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HRL03180.1"
                     /translation="QAGVDLKQAHMTAIHQVHISQQGAFGRTLLSEADLKLPFMGQVV
                     DYARLMQACMHALDTQHIASHWGEAVEHVSTLSNMAQVTLVNGAQITCDWLILAEGGQ
                     LTAQLPELAPQQYDYQQRAYITTAHFDRPQDGVAYERFAHDGPFALLPYGDNYRLIWT
                     RSPAEAERLASLPFAEFKAIFHAAFGKRAGELVAVDPPIQFPLVLKQLQKVTTGRVIC
                     IGNAAQTMHPVAAQGLNLGVRDAQALAQAFTHTTQVKNGSLGSHYARARRLDAKSIVA
                     FTHSLVTAFDQPSAVLQAGRGMVMSALNTSLPLRQKFSEHLIFGL"
     gene            983..>1654
                     /locus_tag="PK856_12240"
     CDS             983..>1654
                     /locus_tag="PK856_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HRL03181.1"
                     /translation="MSIAYFDGVIVGGGLVGAALAVALAEQGKEIALLELRPPQLDLQ
                     ADAKDWDARIYAISPANEQFLRSLNAWPDMHRVQPVSHMDVRGDAGGKIEFSAPAIHT
                     SHLTSILENRWLLQAIWQRIKGMDNIHVFSATAQSLNSDEHAASVTLHDGQVLSAPLI
                     MAADGANSWVRTQTDIKVDAKPYHHHGIVANYAIEKPHGNIARQWFKQGDVLAYLPLP
                     QERISI"
BASE COUNT          392 a          479 c          411 g          372 t
ORIGIN      
        1 acaagcgggc gtggatttga aacaagcaca catgaccgcc atccaccaag tacatatttc
       61 tcaacaaggc gcgtttggtc gcaccttatt atctgaagcc gatttaaagc tgcctttcat
      121 gggccaggtg gtcgattacg cccgtttgat gcaagcgtgt atgcacgctt tagatacgca
      181 acacatcgcc agccattggg gcgaggcggt agaacacgtg tctaccttgt ccaatatggc
      241 gcaagtaacg ctggtcaatg gtgcacaaat cacctgtgac tggctcatct tagccgaagg
      301 cgggcaattg acggcacaat tgcctgagct cgcgccacaa caatacgatt accaacaacg
      361 tgcctacatc accaccgccc atttcgaccg cccacaagac ggcgtggcgt atgagcgctt
      421 tgcccacgat ggcccgtttg ccttattgcc ttatggcgac aattaccgcc tgatttggac
      481 gcgctcgcca gcagaagctg aacgtttggc cagtttgccc tttgccgaat tcaaagccat
      541 cttccacgcc gcttttggca agcgtgcggg cgaattggtg gcggtagacc cacccataca
      601 atttccattg gtgttaaaac aattgcaaaa agtcaccact ggccgcgtca tctgcatcgg
      661 caatgccgca caaaccatgc acccagtggc cgcacaaggc ttgaatttgg gcgtgcgcga
      721 tgcacaggct ttggcacaag ccttcaccca caccacccaa gtcaaaaacg gcagcttggg
      781 cagccactat gcgcgtgcac gccgtttgga tgccaaatcc atcgtcgcct tcacccactc
      841 actggtcacc gctttcgacc aaccgagcgc agtattgcaa gcgggtcgtg gcatggtaat
      901 gagtgcgctc aacacctcat tgcctttgcg acaaaaattc tctgagcatt tgattttcgg
      961 tttgtaaagg cggcctgtaa acatgagtat tgcgtatttt gatggtgtga ttgttggtgg
     1021 tggcttggta ggcgcggcct tggcagtggc tttggccgaa caaggcaaag aaatcgcctt
     1081 attggaattg cgtccaccgc aattggattt acaggccgat gccaaggatt gggatgcgcg
     1141 catttatgcc atcagccctg ccaatgagca atttttgcgc agcctcaatg cttggccaga
     1201 catgcacagg gtacagcccg tcagccacat ggatgtgcgc ggcgatgccg gtggcaaaat
     1261 cgaattttcc gcccccgcca ttcacacctc acacctgacg tctattttgg aaaatcgttg
     1321 gctgttgcaa gccatatggc aacgcattaa gggcatggac aacatccatg ttttcagcgc
     1381 taccgcccag tcattaaaca gcgacgaaca cgccgccagc gtgaccttac acgatggcca
     1441 agttttatct gcgcccttaa tcatggcggc cgatggtgcc aattcgtggg tgcgcaccca
     1501 aaccgacatc aaagtcgatg ccaagcctta ccaccatcac ggcatcgtcg ccaattacgc
     1561 catcgaaaaa ccgcatggca acattgcccg ccaatggttc aagcaaggcg atgtattggc
     1621 ctacttgccc ttgccgcaag agcgcatctc catc
//
LOCUS       DAOMVK010000512         1653 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_21452_length_1653_cov_2.009387, whole genome shotgun sequence.
ACCESSION   DAOMVK010000512 DAOMVK010000000
VERSION     DAOMVK010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21452_length_1653_cov_2.009387"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="PK856_12245"
     CDS             complement(<1..636)
                     /locus_tag="PK856_12245"
                     /inference="COORDINATES: protein motif:HMM:NF012273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRL03182.1"
                     /translation="MKPILFVGHPLGSSLGLVAALETLKISYQFARVSMPADFQSAAY
                     KNLNDRQQTPVLMLSDGTILTETMAIAQWLMHQDREHQISFPQPSSAELDLIKSMAFI
                     NSSYTTSFIPLWLSMEVDSLSETDTSVLHKIGTMLTDKSQKQLENLITKSPFFYGNAP
                     TLTDFLFAGVGRWADFHQVFDLQAYPKTAQWKEAIFALPQIQLAEQIEQGSI"
     gene            739..1635
                     /locus_tag="PK856_12250"
     CDS             739..1635
                     /locus_tag="PK856_12250"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRL03183.1"
                     /translation="MMTANPLFLFCKYVEAGSLSAASRNLNVSKSTLSKSLADLEAHL
                     QVKLVHRSTRSFKLTEVGEEIYQHARILCDELNLIDNIAKSRNAKPFGRIRISASMPI
                     VQHHLAHLIPQFATSYPEIVLDVVTSDQYSNLIDENIDIAIRSHSKILENSAHLYGKQ
                     LLKDPIILVAGRKYLARHGQPELPSELQSHHSIMTANVHWQLYKNGQEAAQVQPKCRL
                     QTNDLNVVIQAILQNLGIAAVPKSFVQKHIATGELAQVLPEMTCGDMVTTVLTTSNKN
                     RLLAVQIFLEFLFQHVQNESVV"
BASE COUNT          479 a          370 c          379 g          425 t
ORIGIN      
        1 gatgctgcct tgctcgattt gttcggccaa ttggatttgt ggcaaggcaa agatggcttc
       61 tttccactgg gccgttttag ggtaggcctg aaggtcaaaa acctgatgaa aatctgccca
      121 cctgccgacg cctgcgaaca gaaaatccgt taaagtcggg gcattgccat agaaaaatgg
      181 gcttttggtg atgaggtttt ccaattgctt ttggctttta tcggtaagca tggtgccgat
      241 tttgtgcaaa accgaagtgt cggtttcgct caaactgtcc acctccatgc tcagccacaa
      301 aggaatgaaa gaagtggtgt aagaggaatt gataaacgcc atgctcttaa tcaaatccaa
      361 ttctgctgat gatggctgag gaaatgaaat ttggtgctca cggtcttggt gcatcagcca
      421 ctgagcgata gccatggttt cggtgagtat tgtgccatct gacagcatca aaactggcgt
      481 ttgctggcga tcattcagat ttttgtaggc ggcgctttgg aaatcggctg gcatggaaac
      541 cctggcaaat tgataggaaa ttttcagggt ttccaatgcg gctaccaagc ccaaagagct
      601 gcctaaaggg tgaccaacga aaagtatggg tttcatgttt tgttcctgtc tttaaaaaca
      661 ttagtttaat ttaatggaca atccatgatt agatggcaat aattgaaact agcgttcttt
      721 atatcgaaca ataaaaccat gatgacagcc aaccctttat tcttattttg caaatatgtg
      781 gaagcaggca gtctttcagc cgccagtcgc aacttgaatg tgtccaaatc caccttgagc
      841 aaaagcctgg ccgatttgga agcacatctt caagtgaaat tggttcacag aagcacccgc
      901 agtttcaaac tcactgaggt tggcgaagag atttatcaac acgcgagaat cttgtgcgat
      961 gagctgaacc tgatagacaa cattgccaaa tcaagaaatg ccaaaccctt tgggcgcatc
     1021 agaatttcag cttctatgcc catcgtccaa caccatctgg cccatttaat cccgcagttt
     1081 gcgacaagct atcccgaaat tgttttggat gtggtcacca gcgaccaata ttcaaactta
     1141 atagatgaaa acatagacat agccatccgc agccactcca aaatcttgga aaacagtgcg
     1201 cacttgtatg gcaaacaact gttgaaagac cccattattt tggtggcagg gaggaaatac
     1261 ctggcaagac acggccagcc agaactgcct tcagaattgc aatctcacca tagcatcatg
     1321 actgccaatg tacattggca attgtacaaa aatgggcaag aagccgcgca agtgcaaccc
     1381 aagtgccgat tgcaaaccaa cgatttgaat gtggtgatcc aggccatatt acagaatctg
     1441 gggattgccg ctgtgcccaa atcgtttgtg caaaaacaca tcgccacagg cgagttggca
     1501 caagtcttac cagagatgac atgtggcgac atggtaacca cggttttgac cacctccaat
     1561 aaaaacagac tgctcgcggt gcaaatcttc ttagaatttc tgtttcaaca tgtgcaaaac
     1621 gaatccgttg tttgaggtta taaagcaagt tcg
//
LOCUS       DAOMVK010000513         1648 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_21519_length_1648_cov_2.782800, whole genome shotgun sequence.
ACCESSION   DAOMVK010000513 DAOMVK010000000
VERSION     DAOMVK010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21519_length_1648_cov_2.782800"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="PK856_12255"
     CDS             complement(<1..607)
                     /locus_tag="PK856_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRL03184.1"
                     /translation="MTQKLVHVVAAVILNEQGEFLLSSRPEGKPYAGYWEFAGGKVEA
                     GETELEALQRELHEELGIHIHAATPWLTKIHHYEHASVLLRFFRVLPSEWQGEVVCRE
                     AQQYSWQQAGRFDVEPMLPANGPILRALSLPLVLRGNTHTGWTGVNGVGEVSMGFTNQ
                     DIRLLPINTNQTDYAGLQLQSLQAASDLQACAADAWWWQPRS"
     gene            complement(604..981)
                     /gene="acpS"
                     /locus_tag="PK856_12260"
     CDS             complement(604..981)
                     /gene="acpS"
                     /locus_tag="PK856_12260"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HRL03185.1"
                     /translation="MIYGIGTDMVDIKRIERLYQQYRDAFALKILSRIEYLEWQSHPQ
                     PVAFLAKRFAAKEAFSKAVNTGIRNPVTLGNIGVIHDELGKPEFWVAEPLQAWMDERG
                     IKHVHLSLTDEGHYSVAFVVAEQ"
     gene            complement(987..>1648)
                     /locus_tag="PK856_12265"
     CDS             complement(987..>1648)
                     /locus_tag="PK856_12265"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HRL03186.1"
                     /translation="LAETHGADLITLHLREDRRHIKDADVAIINQALKTRMNLEMALT
                     DEMLEHALKIKPADVCLVPEKREEVTTEGGLDVLKYAKKITKFTRILTDAGIRVSLFI
                     DADADQIIAARDVGAPVIELHTGAYADAANHEARQHELGRIAAGAELGDELGLVVNAG
                     HGLNIHNVSEIAKLPQIRELNIGHALIAQAVFLGLPEAIKQMKKVMYRARISAGITEG
                     L"
BASE COUNT          409 a          474 c          348 g          417 t
ORIGIN      
        1 cactgcgcgg ctgccaccac caagcatcgg cagcgcatgc ctgcaaatca gatgcggcct
       61 gtaagctttg taattgcaaa cccgcataat cggtttgatt tgtgttaatt ggcaataagc
      121 gtatgtcttg atttgtaaaa cccatgctca cttcgccgac accattcaca ccagtccaac
      181 ctgtatgtgt gttgccacgc aatacaagcg gcaaactcaa tgctcgcaaa atcgggccat
      241 tggcaggcaa cattggctcc acatcaaaac gcccagcctg ttgccaagag tattgctgtg
      301 cctcacggca caccacctca ccttgccatt cgcttggcaa caccctaaaa aagcgcaaca
      361 acacactggc atgctcataa tggtggattt tggtcagcca tggcgtggcg gcgtgaatgt
      421 gtatgcccaa ttcttcatgc agctcacgtt gcaaagcctc aagctcggtt tcacctgcct
      481 ccactttgcc accggcaaat tcccaataac ctgcgtaagg tttgccttca gggcgtgagc
      541 tcagcaaaaa ttcgccttgc tcattcaaga tgactgccgc caccacatgc accaattttt
      601 gtgtcattgc tcggccacca caaaagccac gctgtaatgg ccttcatcgg tcaaactcaa
      661 atgcacatgc ttgatgcctc gctcgtccat ccacgcttgc aaaggctcgg ccacccaaaa
      721 ttcaggttta cccaattcat cgtgtatcac gccgatgttg cccaaagtca cgggattgcg
      781 tatgcccgta ttcacagctt tgctgaatgc ttctttggct gcaaaacgct tggccaaaaa
      841 ggccacaggt tgcggatggc tttgccattc caaatactca atgcgtgaca aaattttcaa
      901 agcaaacgca tcgcggtatt gttggtacaa gcgttcgatg cgtttgatgt ccaccatatc
      961 agtgcctatg ccgtaaatca ttccttctat aacccttctg tgatacctgc actgatgcgt
     1021 gcacggtaca tgactttttt catttgtttg atcgcttcag gcaagcccaa aaacaccgct
     1081 tgcgcaatca gagcatgacc aatgttcagc tcacgaattt gtggcaactt ggcaatttca
     1141 gacacattgt gtatgttcaa accatgccca gcgttcacca ccaaacccag ctcatcgccc
     1201 aactctgcgc cagccgcaat gcgtcccaac tcgtgttgac gcgcctcatg gttggctgca
     1261 tcggcataag cacctgtatg cagctcaatc actggagcac ccacatcgcg cgcggcaatg
     1321 atttggtcgg catcggcatc aataaacaaa gacacacgta tgccagcatc ggtcaatata
     1381 cgggtgaatt tggtgatttt tttggcgtat ttcaaaacat ctaagccacc ttcagtggtc
     1441 acttcttcac gcttttcagg caccaagcac acatcggcag gtttgatttt caacgcatgt
     1501 tccaacatct catccgttaa agccatttcc aaattcatgc gcgttttcaa ggcctgattg
     1561 ataatggcca catcggcatc tttgatgtga cggcggtctt cacgcaaatg caaggtaatc
     1621 aagtccgcac cgtgggtttc cgccagca
//
LOCUS       DAOMVK010000514         1645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_21567_length_1645_cov_2.626415, whole genome shotgun sequence.
ACCESSION   DAOMVK010000514 DAOMVK010000000
VERSION     DAOMVK010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21567_length_1645_cov_2.626415"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /gene="ccoP"
                     /locus_tag="PK856_12270"
     CDS             complement(<1..822)
                     /gene="ccoP"
                     /locus_tag="PK856_12270"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HRL03187.1"
                     /translation="MNNSDFTSGYWHYHIAFIIVLAIIGLVWLLWSQKAAKTNKPDES
                     DTTTGHSWDGIEEYNNPMPKWWFYLFVLTMVWGIGYLVAYPGFGETKGMLGWSSQGQY
                     EQEVKKADDEFGVIYKKYANMPLEQVADDAEARKIGQNLFNTYCIQCHGSDAKGSKGF
                     PNLTDSDWLWGGDPATIHESIKNGRIGVMAAWGPALGEERVKDVANYVMSLSRDKGFD
                     EGRAERGQVVFNSLPANCASCHGENGKGTLGMAPNLTDDVWLWGNSEKNIIDTITN"
     gene            complement(819..1013)
                     /locus_tag="PK856_12275"
     CDS             complement(819..1013)
                     /locus_tag="PK856_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HRL03188.1"
                     /translation="MDVTWARISFTFLIIISFFVVVWIACAKQSKQRYNDIARQIVDD
                     DDSVATDVPQSHPDATEQKQ"
     gene            complement(1020..1631)
                     /gene="ccoO"
                     /locus_tag="PK856_12280"
     CDS             complement(1020..1631)
                     /gene="ccoO"
                     /locus_tag="PK856_12280"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HRL03189.1"
                     /translation="MSLQKLAEEKVGFLIVFVLLVISFGLLIEIVPLFFSKSVTEPAP
                     GVKPYTPLQIAGRDVYIREGCYTCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRMGPDLARVGGRYSDEWHRLHLLNPRQVVPESNMPAFPHLTRNAVDAAVVERNMRGL
                     AKVGVPYTDADYKSVAEEVNGKSEMEAVIAYLQGLGLALKNVR"
BASE COUNT          414 a          434 c          379 g          418 t
ORIGIN      
        1 attggtgatg gtgtcgatga tgtttttctc gctattgccc cacaaccaaa catcgtcggt
       61 gaggttgggc gccatgccca aagtgccttt gccattttca ccatgacaag aagcacagtt
      121 ggcaggcaag ctgttgaaca ccacttgtcc acgctcggca cggccttcat caaaaccctt
      181 gtcacgggat aaagacatca catagttggc cacatctttc acacgctctt cacccaaagc
      241 cgggccccaa gcagccatca caccgatgcg gccatttttg atggattcat ggatggtggc
      301 aggatcgccg ccccacagcc aatcgctgtc ggtgaggtta gggaagcctt tagagccttt
      361 ggcatccgag ccgtggcatt ggatacaata ggtattgaac aggttttgac caattttgcg
      421 tgcttctgca tcgtcagcca cttgttctaa cggcatgttg gcgtattttt tgtaaatgac
      481 accaaattcg tcgtcggctt ttttcacttc ttgttcatac tggccttggc tgctccagcc
      541 caacatgcct ttggtctcgc caaagccagg gtaggccacc aaatagccta tgccccaaac
      601 catggtcaaa acaaacaggt aaaaccacca tttgggcatg gggttgttgt actcctcaat
      661 cccatcccag ctgtgtcccg tggtggtgtc gctttcatcg ggcttgttgg ttttggcggc
      721 tttttgcgac cacaacagcc acaccaaacc aatgatggcc aagacaataa taaaagcgat
      781 gtggtaatgc caatatccgc tggtgaaatc tgaattattc attgtttttg ctccgttgca
      841 tctggatgtg actgcggcac atcggttgcg actgaatcat cgtcatccac aatctgacgg
      901 gcgatgtcgt tgtagcgctg cttagattgc ttcgcacaag cgatccacac caccacaaaa
      961 aaactgatga tgattaaaaa tgtgaatgag atacgtgccc aagtgacatc catgatgttt
     1021 taccttacgt ttttcaaagc gagacccaaa ccttgtaagt aggcgatgac tgcttccatt
     1081 tcagatttgc cattgacctc ttcagctacc gacttgtaat cggcatctgt gtaaggcacg
     1141 cccactttgg ccaaaccgcg catattgcgc tccaccactg ccgcgtctac cgcattgcga
     1201 gttaagtgag ggaaggctgg catattggat tcaggcacca cttggcgcgg attcaacaaa
     1261 tgcaaacggt gccattcgtc tgaataacga ccgcccactc gagccaagtc aggccccatg
     1321 cgttttgagc cccattggaa agggtggtcg tacaccgatt cacctgccac agaatagtga
     1381 ccatagcgct cggtttccgc acggaatgga cgtatcattt gtgaatgaca ggtgtaacag
     1441 ccttcacgga tgtagacatc gcggcctgca atttgcaaag gcgtataagg cttcacacca
     1501 ggtgcgggtt cggtcaccga tttagagaaa aacaaaggca caatctcgat caataagcca
     1561 aaactgatga ccaacaacac aaacacgatg agaaagccga ctttttcttc cgctaatttt
     1621 tgtaaactca taacagtctt cctta
//
LOCUS       DAOMVK010000515         1637 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_21723_length_1637_cov_2.606195, whole genome shotgun sequence.
ACCESSION   DAOMVK010000515 DAOMVK010000000
VERSION     DAOMVK010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21723_length_1637_cov_2.606195"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>380)
                     /locus_tag="PK856_12285"
     CDS             complement(<1..>380)
                     /locus_tag="PK856_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003786168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRL03190.1"
                     /translation="LSAAHTLALVKSVMSDKQIEEFEATKECNFAISPSGIGRFRVNA
                     FIQQGSAGMVMRVIPTELPTIDGLGAPQILKEVVMSKRGLCILVGGTGSGKSTTLAAM
                     VDYRNEHSFGHIITIEDPVEFVHP"
     assembly_gap    381..390
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<391..506)
                     /locus_tag="PK856_12290"
     CDS             complement(<391..506)
                     /locus_tag="PK856_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HRL03191.1"
                     /translation="MDRGQAYQYVCELLKVMLTQKSSDLFITANYPPAMKIDG"
     gene            complement(774..>1637)
                     /locus_tag="PK856_12295"
     CDS             complement(774..>1637)
                     /locus_tag="PK856_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009119127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3734 domain-containing protein"
                     /protein_id="HRL03192.1"
                     /translation="VAESSFEAFRTMLEGQRGFFKPRGFIPFDATPNHLSYYTTDKLK
                     DTLAEFVDLKLLNNPKNMKVSVGAVNVRTGNYTVFCNENMELTFDHIIASGALPPGFP
                     AVEIDGDYYWDGGLVSNTPLDDLIENPQSINQLIFQVDLWDARGQVPENLIGIDERIK
                     DIQYSSKTRYATTVMKKRHSFNHLVKELLKYIPEDQRNVACFKEAEAMTDVGFTNVFQ
                     LIYRKKAFERGHKDYEFSAHTMYEHWNSGLDDIKATLRQEPWFEMSETGEIFEQHDIH
                     KVRRYSYDNDL"
BASE COUNT          408 a          343 c          392 g          484 t
ORIGIN      
        1 cgggtgaacg aattcgactg ggtcttcgat ggtgatgatg tggccaaacg agtgttcatt
       61 gcggtaatcc accattgcgg ccagggtggt cgatttgcct gaacccgtac cgcccaccaa
      121 aatacacaag ccacgtttgc tcatcacgac ttctttgagg atttgtggcg cacccaagcc
      181 atcaatggtg ggtagttctg tcggaatcac acgcatgacc atgcccgctg aaccttgttg
      241 tatgaatgca ttgacgcgaa agcggccgat gccactcgga gagatggcaa aattacactc
      301 tttggtggct tcaaattctt cgatttgctt gtctgacata accgatttaa ccaaggccaa
      361 agtgtgggct gcgctgaggt nnnnnnnnnn ccatcaattt tcatcgcagg agggtagttg
      421 gcggtaatga ataaatcact gcttttttga gtcagcatga ctttgagcaa ttcacagaca
      481 tattgatagg cttgaccgcg gtccatttgt attattccct taagttttat tgtttggtga
      541 gagcttcacc tgattggcag tggatacagc aaataccacg cctgtttggt ggcatcatac
      601 ggatttttac atgatatatg aatgaaaata tttttatatt tttgatttta tagagataaa
      661 aaatttatat ttaaagatga tttgatgatt ttgatgtggt tgacaggcat ggtgtgcaca
      721 ataaagcggc ctgtaattgt ttacaggccg cttttaatag aacaatacgt gttttataaa
      781 tcgttgtcgt agctgtaacg acggacttta tggatgtcgt gttgctcgaa gatttcgccc
      841 gtttccgaca tttcaaacca tggctcttgg cgcaaggtgg ctttgatgtc atctaagcca
      901 ctgttccaat gctcatacat ggtgtgtgca ctgaattcgt agtctttgtg gccacgctca
      961 aatgcttttt tgcggtaaat caattggaac acattggtaa aaccaacatc ggtcatggct
     1021 tcggcttctt tgaagcaggc cacattgcgt tggtcttcgg ggatgtattt caacaattct
     1081 ttgaccaagt ggttaaagct gtggcgcttt ttcattaccg tggtggcata acgcgttttg
     1141 ctcgagtatt gaatgtcttt gatgcgctca tcaatgccaa tcaaattctc aggcacttgg
     1201 ccgcgggcat cccacaagtc cacctgaaaa atcaattggt tgatggattg cgggttctca
     1261 atcaaatcgt ctaaaggcgt gtttgatacc aagccgccat cccaatagta atcgccatcg
     1321 atttctaccg caggaaaacc tggcggcaaa gcgccagaag cgatgatatg gtcgaaggtc
     1381 aattccatgt tttcattgca gaataccgtg taattgcctg tgcgcacgtt caccgcaccc
     1441 actgaaacct tcatgttttt gggattgttc agcagtttca aatccacaaa ctcagccaaa
     1501 gtgtctttga gtttgtctgt ggtgtaatag ctgaggtgat tgggtgtggc atcaaaggga
     1561 atgaaaccac gtggtttaaa gaagccgcgt tgaccttcaa gcatggtgcg gaaggcttca
     1621 aaactggatt cggccac
//
LOCUS       DAOMVK010000516         1635 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_21765_length_1635_cov_1.861392, whole genome shotgun sequence.
ACCESSION   DAOMVK010000516 DAOMVK010000000
VERSION     DAOMVK010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21765_length_1635_cov_1.861392"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1491)
                     /locus_tag="PK856_12300"
     CDS             complement(<1..1491)
                     /locus_tag="PK856_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRL03193.1"
                     /translation="MAAAGKQANDTLQDNIDKLPEQIDAISKEIREWGIFDLFTDSIA
                     RREWTSHLIERSLNSTAGWVEFLCALAMIAAGYFLLDNARKRHIQKMALAGQGVIKLN
                     FFKHLLTRLALPTLLGLTSFLIIKGMEYFDLKTLWFGLVIVAAAWMAMIRIVCAILYY
                     TLPAKIFNHKTEVILSSTIWIVFLMWISGATDWIINWAKSVKLSFAKDSLSLFDIFNG
                     VVWVVIIMMLAMWLAKVIEGRLMGFTRMDMSLRIALSNITKTLFIVLSFLIALPMVGI
                     DLTVLSVFGGALGVGLGFGLQKVASNYVSGFIVLFDRSVRVGDRVNVNNFNGYVTKIT
                     TRYTVVKDANGAEALVPNENFIANMVINESYSGKALWRTIPIQVAYNTDLSKALAIMK
                     AAAEKQERVRDDASATLAGFADSGINLNVGFWVKDPENGFSLLNSNIMMDIWQQFNEA
                     GIEFPYPQREIRILNSEPTVETPPPKVNIPTSKEATISNEPSSAQAQ"
BASE COUNT          416 a          398 c          410 g          411 t
ORIGIN      
        1 ctgtgcttgt gcgctactgg gctcattaga aatggtggcc tctttggaag tggggatgtt
       61 gaccttcggt ggcggagttt ccactgtggg ctcgctgttt aagatgcgga tttcacgttg
      121 tgggtatggg aactcaatgc ctgcttcgtt aaattgttgc caaatgtcca tcatgatgtt
      181 ggagttgagc agagaaaaac cattctcagg gtctttcacc caaaagccca cattcaaatt
      241 gatgcctgag tcggcgaagc cagccaaagt tgctgaggca tcgtcacgca cccgttcttg
      301 cttttccgct gctgctttca tgatggccaa agccttgctc aagtcggtgt tgtaagccac
      361 ttgtatcgga atggtgcgcc acagcgcttt gccgctgtaa gattcgttaa tcaccatgtt
      421 ggcaatgaag ttttcattcg gcaccaaggc ttcagcgcca ttggcgtctt tgaccactgt
      481 gtagcgggtg gtgattttgg tgacgtagcc attgaaattg ttgacgttga cacggtcgcc
      541 cacgcgcacg gagcggtcga acagcacaat aaagcccgac acataattgc tggcaacttt
      601 ttgcaaacca aaacccaagc ccacacccaa agcaccaccg aacaccgaca ataccgtcaa
      661 atcgatgcca accatgggca aggcaatcag gaaagacaaa acgatgaaca gggttttggt
      721 gatgttagac aaggcaatgc gcaaactcat gtccatgcgc gtgaagccca tcaagcggcc
      781 ttcaatcact ttggccagcc acatcgccaa catcatgatg atgaccaccc acaccacgcc
      841 attgaagatg tcaaacaggc ttaagctgtc tttggcaaag ctgagcttga ccgatttggc
      901 ccaattgatg atccaatcgg ttgcgcccga aatccacatc aaaaacacaa tccaaatggt
      961 ggaagacaag atgacttcgg ttttgtggtt gaaaatcttg gcgggcaggg tgtaatacaa
     1021 aatcgcacac acgatgcgta tcatcgccat ccacgccgcg gccacaatga ccagcccaaa
     1081 ccacagcgtc ttcaaatcaa aatactccat gcctttgatg atgaggaatg aagtgaggcc
     1141 aagcaaggtg ggcaaagcca agcgggtcag caagtgtttg aagaaattga gcttgatcac
     1201 gccttggcct gccagcgcca ttttttgaat gtggcgcttg cgggcattgt cgagcaggaa
     1261 ataacctgcg gcaatcatgg ccagcgcgca caagaattcc acccagcctg cggtcgaatt
     1321 caaactgcgc tctatcaaat ggctggtcca ttcgcgtctg gcaatggaat cggtgaacaa
     1381 gtcaaaaata ccccattcac ggatttcttt ggaaatggcg tcgatttgtt cgggcagttt
     1441 gtcgatgttg tcttgtaatg tgtcgtttgc ttgtttgcca gcggctgcca tacatgaata
     1501 cctaatgaag ggtgttgatg ggtgtgaggc ttgagcccac tgtaaatatt gtagctgaca
     1561 aaaatcgcat cgttgcgttt tttatctctc ttaacagttt taaacctgtc ttgtattggc
     1621 aggtgtccat ggttt
//
LOCUS       DAOMVK010000517         1633 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_21788_length_1633_cov_2.503169, whole genome shotgun sequence.
ACCESSION   DAOMVK010000517 DAOMVK010000000
VERSION     DAOMVK010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21788_length_1633_cov_2.503169"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PK856_12305"
     CDS             <1..288
                     /locus_tag="PK856_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL03194.1"
                     /translation="AHFWGIVAVQIALGFMLALNDGCLPAILSELFPTDVRYSGFAFT
                     FNTAMALLGGTSPLFATWLIQQTHSPLAPAWYLIAISLISLVALVNIKKLY"
     gene            439..960
                     /locus_tag="PK856_12310"
     CDS             439..960
                     /locus_tag="PK856_12310"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRL03195.1"
                     /translation="MIRAATAADLDAIVSGYQAHFAHEREHGAYTVFQEGVYPTRAHA
                     EAALQAQGLYVYTSENQVLASLILHAAQPAEYQHINWPSTALPEQIWVLHLLMVHPSL
                     AGQGIGARLVRYGLDLAAAHCCTAVRLDTGAQNLPAVALYQKLGFQLVAQSDMQVGGA
                     IAHANHLFFEKSL"
     gene            complement(1203..>1633)
                     /locus_tag="PK856_12315"
     CDS             complement(1203..>1633)
                     /locus_tag="PK856_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HRL03196.1"
                     /translation="KSVLITDWRCDWVAPGIAELLVKQGFDVSIAINGLCLGETLPLY
                     VRDELTATAQRLGIKLLPNARLYGYDNGTVYLQHNTSAEALELEGIDTIIACDGQISL
                     DELGDAIEDLGMVLHRIGDCNTPRTAEEAMYEGLKVAAMI"
BASE COUNT          394 a          471 c          369 g          399 t
ORIGIN      
        1 gcccactttt ggggcatcgt ggcggtacaa attgccttgg gcttcatgtt ggccttgaac
       61 gatggctgtt tgcctgccat cctcagtgaa ttgttcccca ctgatgtgcg ttactcaggt
      121 ttcgccttca ctttcaatac cgccatggcc ttgctgggcg gcacatcgcc actgtttgcc
      181 acttggttga tacaacaaac ccactcacct ttggcaccgg cctggtattt aatcgccatc
      241 tccctgattt ctttggtggc tttggttaat attaaaaagc tgtattgatt acccttgcta
      301 catacaagcg gcctgtaaac tgtgttacgg tttacaggcc gcttttgtgt ttgccatcga
      361 tagcctcacc caaatacaac gaatcaccta tcatttgctt gccttgtaaa ccacatcaca
      421 gaaaatttgg aaaacaccat gatacgtgcc gccaccgccg ccgatttgga tgctattgtt
      481 tcaggttacc aagcccattt cgcccacgag cgagaacacg gcgcttacac cgtgtttcaa
      541 gaaggtgttt accctacccg cgcccatgcc gaagccgcgc tgcaagcaca aggtttgtat
      601 gtgtacacaa gcgaaaacca agtcttggcc agcctcatct tgcacgcagc ccaacccgct
      661 gaataccaac acataaactg gcccagcact gcactccccg aacaaatatg ggttttacac
      721 ctgctgatgg tacacccgag cttggcagga caaggcatag gcgcacgctt ggtgcgttac
      781 ggattagact tggcagcagc acactgctgc accgctgtga gattggacac aggcgcgcaa
      841 aatctgcctg ccgtggcttt gtatcaaaaa ctgggttttc aattggtggc gcaatccgac
      901 atgcaagtgg gcggcgccat tgcccacgcg aaccatttgt tttttgaaaa aagcctgtaa
      961 tacaacaccc aagcggcctg taaacggttt taaagtttac aggccgcatt agcgtgaaca
     1021 acacgcacac aaaaatatgg ctgcccttgc ttatcagtcc agttttaaaa gccttgccca
     1081 ttctcacagc cagcttgttt cagtacatgg ccgcatcagc ctactcacac tagccataca
     1141 aagcggcctg taaaccgttt tacacgttta caggccgctt aaagtacaac atgcgacggt
     1201 gcttaaatca tggcagccac tttcaaaccc tcatacatcg cttcttcggc ggtgcgtggg
     1261 gtgttgcaat cgccaatgcg gtgcaacacc atgcccaagt cttcaatcgc gtcgcccaat
     1321 tcgtccaagg aaatttgacc atcgcaggcg atgatggtgt ctatgccttc caattccagc
     1381 gcttcggcgc tggtgttgtg ctgcaaatac accgtgccat tgtcgtagcc gtacaaacgc
     1441 gcattgggca agagtttgat gcccaagcgc tgcgctgtgg cggtcaactc atcacgcaca
     1501 tacaagggca aggtttcgcc caaacacaag ccattgatgg cgatgctgac gtcaaagcct
     1561 tgcttcacca acaattcagc gatgccaggc gccacccaat cgcagcgcca atccgtaatc
     1621 aataccgact tgc
//
LOCUS       DAOMVK010000518         1631 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_21825_length_1631_cov_2.174492, whole genome shotgun sequence.
ACCESSION   DAOMVK010000518 DAOMVK010000000
VERSION     DAOMVK010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21825_length_1631_cov_2.174492"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1631
                     /locus_tag="PK856_12320"
     CDS             <1..>1631
                     /locus_tag="PK856_12320"
                     /inference="COORDINATES: protein motif:HMM:NF024958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HRL03197.1"
                     /translation="LPFVNGVEVSITWNSKVIHIVGLNMRDDAPELQAALKKEKRETA
                     RAAILTTLKKLGVDISSHLAPKVLLAEAESGLKGKMPKSLDAWLKVDALPELVWQDSG
                     KAVDAKITLWWVVLAQKLKEPGGNELLDLYLEQTTPASRARLAEFLLHGFINEDTRHP
                     SHTEAEEQAQKAAPQRFKYMQQWYKQYPEYYGQYANATLEMAVTEIKREVMAQYLGSA
                     IADKGLLALTAAVPATTWVQLLQTYMKEHQQRRAQIEAMLMAAAKNNDPAIIQFILSI
                     ARRYKTASVQAKANELIAVIAERNGWSSDELADRTIPTAGLDDSGVLTLSYGEREFTG
                     RLDEDLKWVLADDTGKTLKAMPNARQNEDAALVKEAKAQFNTSKKELKQVLELQTERL
                     YEAMCGSRVWSRADWSEYLQAHPLMQRLLQRLLWQGLNAEGEVVFTFRPTEDGSFINA
                     DDDEIDLQAASGIRLAHTVLLGPEQTQVWQAHLKDYRIKPLFEQLKRELPPEITPKQQ
                     EIDSFRGYLSDTYTLRGVFTKMGYQRGPAEDGGSF"
BASE COUNT          433 a          414 c          452 g          332 t
ORIGIN      
        1 acttgccgtt tgtcaacggc gtggaagtgt cgattacttg gaacagcaaa gtcatccaca
       61 tcgttggctt gaacatgcgc gatgatgcac ccgaattaca ggccgcattg aaaaaagaaa
      121 aacgcgaaac cgcgcgcgcc gcgattttaa ccacattgaa aaagctgggc gtggacatca
      181 gctcgcattt ggcgcccaaa gtcttattgg cagaagccga aagcggcctg aaaggcaaga
      241 tgcccaagtc tttggatgcg tggctcaaag tggatgcctt gcctgaattg gtgtggcaag
      301 acagcggcaa agccgtggac gccaaaatca ccttgtggtg ggtggtgttg gcgcaaaagc
      361 tgaaagagcc aggcggcaat gagttgctcg atttgtattt ggagcaaacc acgccagcga
      421 gccgcgcgcg tttggcggag tttttgttgc atggcttcat caatgaagac acgcgccacc
      481 ccagccatac cgaagcggag gaacaagccc aaaaagcggc gccacaacgc tttaaataca
      541 tgcagcaatg gtacaaacaa tatcccgagt attacggcca atatgccaat gccactttgg
      601 aaatggccgt gaccgaaatc aaacgagagg ttatggcgca gtatttgggt tcggccattg
      661 ccgacaaagg cctcttagcc ttgacagcgg ccgtgccagc cacgacatgg gtgcaattgt
      721 tgcaaaccta tatgaaagag catcaacagc gccgcgcaca aatcgaagcg atgttgatgg
      781 cggcggcgaa aaacaatgac ccagccatca tccaattcat cttgagcatt gcccgccgtt
      841 acaaaaccgc ttcggtacaa gccaaggcca acgaattgat tgcggtcatc gccgagcgca
      901 atggctggag cagcgatgaa ttggccgacc gcaccattcc caccgcaggt ttggatgaca
      961 gcggcgtttt gaccttgagt tatggtgagc gtgagtttac aggccgcttg gatgaagact
     1021 tgaaatgggt attggccgat gacacaggca aaaccctcaa agccatgcca aacgcgcgtc
     1081 aaaacgaaga tgcggccttg gtgaaagaag ccaaagcgca attcaacacc tctaaaaaag
     1141 aattgaaaca ggtattggag ctgcaaaccg agcgtttgta cgaggcgatg tgtggcagcc
     1201 gcgtgtggag tcgggcggat tggtctgagt atttgcaagc gcatcccttg atgcaacgcc
     1261 tgttgcagcg cttgctctgg caaggcttga atgccgaggg tgaggtggtg tttaccttcc
     1321 gccctacgga agatggcagc ttcatcaatg ccgatgacga cgaaatcgat ttgcaggccg
     1381 catctggcat acgcttggcg cacaccgtat tgttaggccc tgagcaaaca caggtgtggc
     1441 aagcgcattt gaaagactac cgcatcaagc ctttgtttga gcagctcaag cgcgaattgc
     1501 cgcctgaaat cacccccaaa caacaggaaa tcgacagctt cagaggctat ttgtccgata
     1561 cttacacctt gcgtggtgtg ttcaccaaaa tgggctacca acgcggccct gccgaagatg
     1621 gcggctcgtt c
//
LOCUS       DAOMVK010000519         1628 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_21887_length_1628_cov_2.490782, whole genome shotgun sequence.
ACCESSION   DAOMVK010000519 DAOMVK010000000
VERSION     DAOMVK010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21887_length_1628_cov_2.490782"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="PK856_12325"
     CDS             complement(<1..991)
                     /locus_tag="PK856_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HRL03198.1"
                     /translation="MSTLAIIISLLLLMFFAYRGISVLILAPIMAAIAILLSGDMIYM
                     MPQYTETFMAALGGYVLKFFPIFLLGALFGQLMVDSGAATSISNWIMQKLGHKRAILT
                     VVIACGILTYGGVSLFVVAFAIYPIAKSMFQAANIPKRLIPGAIALGSFTFTMTALPG
                     TPSIQNAIPIPYFGTNVFAAPGIGIISGLVMFGLGAMWLQSRANKAQSKGEGYGVDTQ
                     ESEKLIDGVTSQGHMPVLLALLPLLLVIGVNALFTYAIFPSVDFSNLKELYPKLEPAK
                     QTGLWSLIIALVVACLSLILFRLGKWNNLKETVNKGVLGSMLPIFNTASDVGYG"
     gene            complement(1416..>1628)
                     /locus_tag="PK856_12330"
     CDS             complement(1416..>1628)
                     /locus_tag="PK856_12330"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRL03199.1"
                     /translation="AIHTMLEAGINFGTIDLNVKMIRALQAHQTYRAEAEVITIGRTL
                     LTAEGRIIGDDGKIHAFGSASCLVIK"
BASE COUNT          474 a          345 c          378 g          431 t
ORIGIN      
        1 caccatagcc cacgtccgat gcggtattga aaatcggtaa catcgaaccc aatacgcctt
       61 tgtttacagt ttctttcagg ttattccatt tacccaagcg gaacaaaatc aagcttaagc
      121 aggccacaac caaggcaatg atgagcgacc acaagccagt ttgtttggca ggttccaatt
      181 ttggatacag ttcttttaaa ttactgaagt ccacgctggg gaagatggca taggtaaaca
      241 aggcgttcac gccaatcacc aataacaaag gcaacaaagc caataacaca ggcatgtggc
      301 cttgtgaggt cacgccatcg atgagttttt cactttcttg tgtgtccacg ccatagcctt
      361 cgcctttgct ttgtgctttg ttggcgcggc tttgcaacca catcgcaccc aagccaaaca
      421 tcaccaagcc tgaaataatg cctatgccag gagctgcaaa cacattggtg ccgaagtaag
      481 gaatcggaat tgcattttga atcgaaggtg tacctggcaa ggcggtcata gtgaaagtga
      541 atgagcccaa ggcaatcgca cccggaatca agcgtttcgg aatgttagcg gcctgaaaca
      601 tggatttggc aatcggataa atcgcaaatg ccaccacaaa caaagacacg ccgccgtaag
      661 tcaaaatgcc gcaggcaatc accacggtta agatggcgcg tttgtggccg agtttttgca
      721 taatccaatt ggaaatgctg gttgcagcgc ctgaatccac catcaattgg ccaaacaaag
      781 cgcccaataa gaaaattggg aagaatttca acacatagcc gcccaaagcc gccatgaagg
      841 tttcggtgta ttgcggcatc atgtaaatca tgtcgccaga cagcaaaatg gcaatggcag
      901 ccatgatggg tgccaaaatc aatacggata tgcctcggta ggcaaaaaac atcaataaca
      961 gcaatgagat gatgatggcc aatgtactca ttgtttctcc tttggttgcg gtgttacgaa
     1021 aacatcagca tgtcgagcac gctgtctttc cttattgaat ttatggggat ttgtcgattg
     1081 gattggttgg tttcagacaa tagacggatg taccatagca tagtgaattt aaattcatca
     1141 attgattttt gattcattaa tattcattca ttgccatttt atgtgaacaa aatcagtatt
     1201 taaccaaatt tcattcataa aatgctgaga taagtgcatc atatgatgca attatgctga
     1261 tgcagagcta gatgtttggg aatgtttata tggaaatgct tggcaacata aattccattt
     1321 gagagagtga cattttgtga aataaagcat gaaaaaacca ccaaacagtg ctttatttgg
     1381 tggtttcaat gtgttggttt aggtatttgt aggatttatt tgatgaccaa gcagctggca
     1441 gagccaaagg catgtatctt gccatcgtca ccgatgatgc ggccttcagc ggtgagcaaa
     1501 gtgcggccta tggtgatgac ttcggcctcg gcgcggtaag tttggtgcgc ttgcaaggcg
     1561 cgtatcattt tgacattcaa atcgatggtg ccaaaattga tgcctgcttc cagcatggtg
     1621 tgtatcgc
//
LOCUS       DAOMVK010000520         1627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_21897_length_1627_cov_3.596692, whole genome shotgun sequence.
ACCESSION   DAOMVK010000520 DAOMVK010000000
VERSION     DAOMVK010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21897_length_1627_cov_3.596692"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="PK856_12335"
     CDS             complement(<1..1136)
                     /locus_tag="PK856_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HRL03200.1"
                     /translation="MSMQEVVDFFNGLIWSKALIYGCLVVGLYFSVRTRFAQVRHFFG
                     MLRLMMDGKSSDAGVSSFQALTMALAGRVGTGNIAGVAAAITFGGPGALFWMWMVAFL
                     GASTAYIESALAQIYKEKHDGQFRGGPAFYIEKGLGIKWYAILFAFSAILACGVLLPG
                     IQANSIADSLKNAMGIDTAVTAAILAVILGFIIFGGVKRIAKFAEIIVPFMALAYIII
                     ALVIVVMNIGELPNVLALIFKSAFGAEAAFGAVVGLAVEWGVKRGVYSNEAGQGTGPH
                     PAGAANVSHPAKQGLVQAFSVYVDTLLVCSATGFMLIITGMYNTVDPNKEGAFLHQGV
                     QNVGVGAGYTQTAMESVLPGFGSIFVAFALFFFAFTTIVAYYYIA"
BASE COUNT          490 a          372 c          369 g          396 t
ORIGIN      
        1 gcgatgtagt aataggccac gatggtggtg aaagcgaaga agaacaaggc aaaagccaca
       61 aagatagaac caaaacctgg caacacgctt tccatcgcgg tttgggtata gcctgcgccc
      121 acgcccacgt tttgcacgcc ttggtgtaag aatgcacctt ctttgttagg gtctaccgtg
      181 ttgtacatgc ccgtgatgat caacatgaag ccagtggcag agcacaccaa caaagtgtct
      241 acatacacag agaacgcttg taccaagcct tgtttggcag ggtgggacac gttggcagca
      301 ccagcagggt gagggccagt accttggcct gcttcgttag aatacacgcc gcgtttcacg
      361 ccccattcaa ccgccaaacc caccaccgca ccaaatgcag cctcagcacc aaaagccgat
      421 ttgaaaatca acgccaatac attaggcaat tcaccaatgt tcatcaccac aatcaccaag
      481 gcgatgatga tgtaagccaa agccatgaaa ggcacgatga tttcggcaaa tttggcaatg
      541 cgtttcacgc caccgaaaat gatgaagccc aaaatcaccg ccaaaatggc cgcagtcacc
      601 gcagtgtcaa tgcccattgc atttttcaag ctgtcggcaa tggagttggc ctgtatgcct
      661 ggcaacaata cgccacacgc caagatggct gagaaggcaa acaaaatggc ataccatttg
      721 atgcccaagc ctttttcaat gtaaaaagca gggccaccac ggaattgacc gtcgtgtttt
      781 tctttgtaga tttgtgccaa agccgattca atgtaggcag tgctcgcacc caaaaaagcc
      841 accatccaca tccagaacaa ggcaccaggg ccaccaaagg taatggccgc tgccacacct
      901 gcgatattgc ctgtgcccac gcgaccggcc aatgccatgg tcaaggcttg gaatgaagaa
      961 acacctgcat ctgaactttt accatccatc atcagacgca acatgccgaa aaagtggcgc
     1021 acttgggcaa agcgggtgcg gacagagaaa tacaggccga ccaccagaca gccataaatc
     1081 agcgccttac tccaaatcaa accattaaaa aagtcgacaa cttcctgcat ggacataatg
     1141 ttctcctttg tgattgtgca tgctgtgagc atgtgtattt gtggcctcat tttaatgagg
     1201 tgtttacaaa agttaaaaca tttttttgca tgttttttgt aaaagtggca tttaatttta
     1261 atgactggtt tgtttgttta tatttgaatc aaatagccaa acagtgatac tgcattgcaa
     1321 taaagtgttg agcctttaga aacaatattg tgaattttag agaaatggct gcgatttcgg
     1381 tggaaatagg gtgatatgct cagatattaa atttatattg tcaaaattgc accaatacgt
     1441 ggcagaagca tgttgggatg aaaatgcaaa atgaatgttt gagttgttgc tatgaatcag
     1501 gcattccaaa aaatgattgc ttgttaatgt gtcgattttt ggctacaaaa cagcgcatac
     1561 tgcgggttgt tgtattgaaa atgcgggcaa aataacaggt ttttaggtca aaactttggt
     1621 tgagtta
//
LOCUS       DAOMVK010000521         1627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_21900_length_1627_cov_2.472010, whole genome shotgun sequence.
ACCESSION   DAOMVK010000521 DAOMVK010000000
VERSION     DAOMVK010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21900_length_1627_cov_2.472010"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1240)
                     /gene="fumC"
                     /locus_tag="PK856_12340"
     CDS             complement(<1..1240)
                     /gene="fumC"
                     /locus_tag="PK856_12340"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HRL03201.1"
                     /translation="MSDVRVETDSFGPIDVPADKYWGAQTERSRHNFKIGGETQPLPL
                     IHALAIVKQAAAEVNLQVGKLDATVANAIVSVAQEIQAGKWDDQFPLVVWQTGSGTQT
                     NMNVNEVISNRAIELLGGDKGSKKPVHPNDHVNMGQSSNDSFPTAMHIATAIEATRLL
                     FPALEVLQAALQTKETEFTHIIKIGRTHTQDATPITLGQEFSGYRAAVEYGQARIVKN
                     LADVYFLAQGGTAVGTGLNSTPEFAADFAAKVATLTGLPFQTAANKFEALASHGALNQ
                     FHGSLNALAADLLKIANDIRFLGSGPRSGLGELSLPENEPGSSIMPGKVNPTQCEALT
                     MVATQVMGNQVTVSIAASQGHFELNVYKPVIAYNVLQSIRLLADAMVSFAEHCVVGIV
                     ANEAHIQQLMENSLMLVTALA"
     gene            complement(1303..1626)
                     /locus_tag="PK856_12345"
     CDS             complement(1303..1626)
                     /locus_tag="PK856_12345"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HRL03202.1"
                     /translation="MQNYEVLKEISDVIEVRKQAAAESSYVSQLLHKGEDAILKKVIE
                     EAGEVLMASKDGDKLHLIKEVADVWFHSMVLLGYHGLRAEDVLMELQRRQNISGIDEK
                     ASRGK"
BASE COUNT          388 a          429 c          388 g          422 t
ORIGIN      
        1 gcgccaatgc ggtgaccagc atcaaagaat tttccatcaa ttgttggatg tgggcctcat
       61 tggccacaat gcccaccacg caatgctcag caaacgacac catcgcatcg gccaacaaac
      121 ggatggattg caacacattg taagcaatca caggcttgta cacattcaat tcaaaatggc
      181 cttggctggc ggcaatgctc accgtgactt gattgcccat cacttgcgtg gcgaccatgg
      241 tcaaggcctc acactgtgtg ggattgactt ttccaggcat gatggatgaa ccaggttcat
      301 tctcaggcaa gctcaattcg cccaaacccg agcgtgggcc tgaacccaaa aagcgaatgt
      361 cgttggcgat tttgagcaaa tcggctgcca aggcattcaa gctgccatga aactgattca
      421 aagcgccatg gctggccaag gcttcaaatt tgttcgccgc ggtttggaaa ggcaagcctg
      481 tcaaggtcgc aactttggca gcaaaatcgg cggcaaactc aggcgtggaa ttcaaacctg
      541 tgcccacggc cgtaccgcct tgtgccaaga aatacacatc ggccaaattt ttcacgatgc
      601 gcgcttggcc atattccact gccgcacggt agcccgaaaa ctcttgtccc aaagtaattg
      661 gagtcgcgtc ttgggtatgg gtacggccga ttttaatgat gtgggtaaat tcggtttctt
      721 tggtttgcaa tgcggcctgt aacacctcca aagcaggaaa tagcaagcgc gtggcttcga
      781 tggcggtggc gatgtgcatg gcagtgggga aagaatcgtt agacgattgg cccatgttga
      841 catggtcatt cgggtgaacc ggttttttac tgcctttgtc accgcccaac aattcaatgg
      901 cacggttgga aatcacctcg ttcacattca tattggtttg cgtaccagaa ccggtctgcc
      961 acaccaccaa aggaaattgg tcgtcccatt ttcccgcttg aatctcttgc gccacgctca
     1021 caatggcatt ggccaccgtt gcatccagtt tgcccacttg cagattcact tcggccgccg
     1081 cctgcttgac gatggccaaa gcatggatta agggcaaagg ctgggtttca ccgccaattt
     1141 taaaattgtg gcgtgaacgc tcggtttgag caccccaata tttgtcagca ggcacgtcta
     1201 tggggccaaa gctgtcggtt tctacgcgta catcagacat ggttgttttc ctcagaagat
     1261 ggttggcttg tttatgaggc aatccacgca atggttatct tgttatttgc cgcgcgaggc
     1321 tttttcgtct atgcccgaaa tgttttgacg gcgttgtaat tccatcaaca catcttcggc
     1381 gcgcaaacca tggtaaccca acaacaccat ggaatgaaac cacacgtctg ccacttcttt
     1441 aatcaaatgc agcttgtcgc catctttgga agccatcaac acttcgcctg cttcttcaat
     1501 gacttttttc aaaatcgcgt cttcgccttt gtgcagcaat tgcgacacat acgaactttc
     1561 cgctgccgct tgtttgcgca cttcaatcac atcggaaatt tcttttaaca cttcgtagtt
     1621 ttgcatg
//
LOCUS       DAOMVK010000522         1623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_21984_length_1623_cov_1.857143, whole genome shotgun sequence.
ACCESSION   DAOMVK010000522 DAOMVK010000000
VERSION     DAOMVK010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21984_length_1623_cov_1.857143"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..697
                     /locus_tag="PK856_12350"
     CDS             191..697
                     /locus_tag="PK856_12350"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRL03203.1"
                     /translation="MKPMYCNDCTLERLPPDAPLPMDLLLLADETTDAIAAYVHDCEV
                     WVVRQQQTMVAVACVLKVNADCVELKNLAVATALQGQGVGSGLLGALKRHYAATYAQM
                     LVGTADVGQAQQRFYQRHGFVRVGTRERFFITHYPEPIIENGQQLVDMHVFACNLHAE
                     DKQAACKP"
     gene            complement(743..1039)
                     /locus_tag="PK856_12355"
     CDS             complement(743..1039)
                     /locus_tag="PK856_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03204.1"
                     /translation="MANFIPAEDPIKPPKLYALEDVKYEGYGDILMATFDYPYANHNM
                     LFLIAYDAAEGSTAIKSLAVNTFPGSGDYITTDETSESETAPASESDNSVAASE"
     assembly_gap    1123..1132
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1133..1453)
                     /locus_tag="PK856_12360"
     CDS             complement(<1133..1453)
                     /locus_tag="PK856_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03205.1"
                     /translation="MHTSIAPTTVWQRSRPLLLCAGLFLLLACTEKPADTAPSAASNT
                     QTTEAAEPSASSAIVKPSKRQLSQGQLDMARSVLEAMKTKQFDAFATHIHPSFAAELK
                     ENPKL"
BASE COUNT          343 a          367 c          502 g          401 t
ORIGIN      
        1 gaatgccgtg cctatggagt acatgaaagc cttgctcacg tcgatgaaac aacttgatgc
       61 cgtggtaaaa agccaagcct tgttggaaaa cgatttgctg aaatacggta agtaatctgc
      121 caaatgagaa gcggcctgta aacttgaatt caaggtttac aggccgcttt gtcatgtgaa
      181 aaacacaaaa atgaaaccca tgtactgcaa tgattgcact ttagaacgct tgccaccaga
      241 tgcgccattg cccatggatt tgctgttgtt ggccgatgaa accactgacg ccattgccgc
      301 ctatgtgcac gattgcgagg tgtgggttgt gcggcagcaa caaaccatgg tggccgtggc
      361 ctgtgtgctg aaagtgaatg ccgattgtgt ggaattgaaa aacttggccg tggcgactgc
      421 cttgcaaggg cagggagtgg gcagtggctt gttgggtgct ttaaaacgcc attacgctgc
      481 gacttatgcg caaatgctgg tgggtacggc cgatgttggg caggcacagc aacgctttta
      541 ccagcgccat ggcttcgtgc gcgtgggcac acgtgagcgc tttttcatca cacactatcc
      601 cgaacccatc atcgaaaacg gccaacaatt ggtggacatg catgtgtttg cctgcaattt
      661 gcatgcagaa gacaaacaag cggcctgtaa accttgatgc gtggtttaca ggccgctttg
      721 tatggggtgg tcgagtgtgt ggctattcgc tggcggccac gctgttgtcg gattcagatg
      781 caggggcggt ttccgattcg ctggtttcat cggtggtgat gtagtcgcct gagccaggga
      841 aggtgttgac ggcgaggctt ttgatggcgg tgctgccttc ggcggcgtca taggcaatca
      901 aaaacagcat gttgtggttt gcataggggt agtcgaacgt ggccattaag atgtcgccat
      961 agccttcgta cttcacgtct tccaaggcat acaatttcgg tggcttgatg gggtcttctg
     1021 cagggatgaa attggccatt tcctctcccc ggcagagccc ggtgatgacc acctgcggcg
     1081 ggccctgctc cgagagcgcc tccgccatgc ggaaaagcgc ctnnnnnnnn nncagcttgg
     1141 ggttttcttt cagctcggcg gcgaagcttg ggtggatgtg ggtggcaaac gcgtcaaatt
     1201 gcttggtttt catcgcttcg agtacgctgc gggccatgtc caattggcct tggctgagtt
     1261 ggcgcttgga gggcttcacg atggcggagg aggcagaagg ttcggctgct tctgtggttt
     1321 gggtgttgct ggcggcgctt ggcgcggtgt ctgcaggctt ttcggtgcag gccaagagca
     1381 gaaacaggcc tgcgcaaagc agcaaaggac gactgcgttg ccaaacagtg gtgggtgcaa
     1441 tggaggtgtg catggtggcc gctcgcagga aatgtcacag gggattgcag ccatgttaat
     1501 cacgacttgt ttgaaactca agcaaatact taaattgtgg ttttatcacc cacaagcggc
     1561 ctgtaaacgg tttacaggcc gcttgtttta ggcttgggtg cgggttttgg cgatgagtaa
     1621 cat
//
LOCUS       DAOMVK010000523         1620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_22031_length_1620_cov_2.118850, whole genome shotgun sequence.
ACCESSION   DAOMVK010000523 DAOMVK010000000
VERSION     DAOMVK010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22031_length_1620_cov_2.118850"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="PK856_12365"
     CDS             complement(<1..602)
                     /locus_tag="PK856_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HRL03206.1"
                     /translation="MSLFFVISPAKNLDEVSPVPVSEQSAIAFPEDTQVLMDALKQLS
                     PQDISALMGVSDKIARLNVERNGKYQIPITLENSGKQALFLFKGDVYEGMDAYHLSAE
                     ALAYLQKHLGILSGLYGLLRPYDLMQAYRLEMGKALKNPRGDNLYQFWGSKITQLINQ
                     RLQAAQADCLINLASQEYFKSVQSTDIAQPIITPVFQDEK"
     gene            774..>1620
                     /locus_tag="PK856_12370"
     CDS             774..>1620
                     /locus_tag="PK856_12370"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRL03207.1"
                     /translation="MAAAQAGSTQVVGSYIALVLIWAATPLAVVWSISGIRPMWGLIG
                     RYTFSLLLMLPLLKLLQQRFPVNRIALKSYAAGSMNLIGGQFFMYLAASYLSSSLIAL
                     MYGFAPLLAGVVGHSILKTQKLKWFQWLGMVIALIGLIVVFLGQQNSGFHIIGVGMMM
                     ISLVSYVFSIFWVKSINAPVPAMAQATGSIAASFLLSMLIVPFVWQYFPSEIPSAKAL
                     SGLAFTVVASSVIAMLCYFSLLRQLAPSTIALTNVMTPIVAMGLGALLNNEHISFTAV
                     CGMVTV"
BASE COUNT          369 a          372 c          405 g          474 t
ORIGIN      
        1 tttttctcat cttggaacac aggggtgatg atgggctgcg caatgtcggt gctttgcacg
       61 gatttgaaat actcttgtga ggccaagtta atcaaacaat cggcttgagc ggcctgtaaa
      121 cgctggttga ttaactgggt aatcttgctg ccccaaaatt ggtacaaatt gtcgccacgc
      181 gggtttttca aagccttgcc catttccaag cggtaggctt gcatcaagtc atatgggcgc
      241 aacaagccat acagaccaga caagatgccc aagtgttttt gtaagtatgc caaggcttct
      301 gcgctcaaat ggtaggcatc catgccctca tacacatcgc ctttgaacaa aaacagcgct
      361 tgcttgccac tattttccag cgtgatgggg atttggtatt tgccgttgcg ctccacattc
      421 aagcgggcaa tcttatcgga aacccccatc aaggctgaga tgtcttgcgg cgaaagctgc
      481 tttaatgcat ccatcaatac ctgtgtgtct tcaggaaagg cgatggcact ttgttcgctc
      541 acaggcacag ggcttacttc atccaggttt ttggcagggg aaatcacaaa aaacaaactc
      601 atctcaacat cctcaatcaa atggtgggtc attttaatca aaataggttt gtttgcccaa
      661 atgtatctgt tgatgtgcag atagtgttta taaatcaatg ttaaaccaag ttaccaaact
      721 agactattgc actgcacaag agaataatgc agcgttttac acgaaagacg tacatggcag
      781 cggcgcaagc agggtctact caagtggtcg gctcttacat tgcattggta ttgatttggg
      841 cggcaacacc gttggcagtg gtgtggagca tcagtggcat acgccccatg tggggcttga
      901 ttggccgcta taccttctct ttgctgttga tgctgccttt attgaagctg ttgcaacagc
      961 gcttccctgt caaccgcatt gccttaaaaa gctatgcagc gggcagcatg aatttgatag
     1021 gcggccaatt ttttatgtat ttggcggcga gttatttgtc gtcgagtttg attgcgttga
     1081 tgtatggctt cgccccattg ctcgcgggcg tggttgggca ctcgatttta aaaacccaaa
     1141 agcttaagtg gtttcaatgg ttgggcatgg taatcgcctt aatcggccta atcgtcgtct
     1201 ttttagggca gcaaaactcg ggcttccaca tcattggcgt gggcatgatg atgatctctc
     1261 tggtgtcgta tgttttttcg attttttggg taaagtcgat taatgcccct gtgcctgcta
     1321 tggctcaggc cacgggctca attgccgcat cgtttttatt gtctatgctg attgtgccgt
     1381 ttgtgtggca atattttccc agcgaaatcc ccagcgccaa ggccttaagc ggcttggcat
     1441 ttaccgtggt cgcgtcttcg gtgattgcca tgttgtgtta tttctcactg ctgcgccaac
     1501 tcgcgccttc tacgattgcc ctgaccaatg tgatgacacc gattgtcgcc atgggattgg
     1561 gcgcattgtt aaacaatgaa cacatcagct tcactgcggt ttgcggcatg gtgacggtga
//
LOCUS       DAOMVK010000524         1615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_22125_length_1615_cov_3.088462, whole genome shotgun sequence.
ACCESSION   DAOMVK010000524 DAOMVK010000000
VERSION     DAOMVK010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22125_length_1615_cov_3.088462"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..924
                     /locus_tag="PK856_12375"
     CDS             238..924
                     /locus_tag="PK856_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRL03208.1"
                     /translation="MPKIAESTATRAEQVFAQLQAAIIRGELAPGSKISEQDLADTYG
                     ISRGPLREALNRLEGQKLLVRMPHVGARVVSLGKRELEELYDVRAELEGMACRLAAAH
                     MSREDVDALYAVLQTHERDPQFQAGTAYFEQDGDYDFHYRIITASGNNTLKKLLCDDL
                     YQLVRMYRIRFSSVPKRPQTALSEHYRIVDALAAGDGELAELLMRRHIQASKRNMASH
                     FDAAQLEETP"
     gene            921..>1615
                     /gene="prpB"
                     /locus_tag="PK856_12380"
     CDS             921..>1615
                     /gene="prpB"
                     /locus_tag="PK856_12380"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HRL03209.1"
                     /translation="MTHITPGQRFRSAVAEEQPLQVVGTLTAYTARMAEAVGYKALYI
                     SGGAVAANSLGLPDLGISTMEDVLIDANRITNATELPVLVDIDTGWGGAFNIARTVKS
                     FIKAGVAAVHMEDQTGTKRCGHRPNKAVVSKEEMIDRIKAAVDARTDDSFVIMARSDA
                     LASEGLDRMIERCLAYVEAGADMVFPEAITSLNMYKKVREAVGVPILANITEFGQTPL
                     FTRDELASANVDI"
BASE COUNT          399 a          380 c          456 g          380 t
ORIGIN      
        1 atgacgtggt agatgccgat tttgaagaag tgaaagacaa aaaagactga gtcttgaatt
       61 gttgacaatc agaaaagaca cctcgtaaaa ggtgtctttt tttgcgccta aaacatgggc
      121 ttttgactgt gtttgtacac gaaccacaat aaattgtaga caatatttgt attaaaactt
      181 gataaatcag tgttgttgga ttaaattcac cctgtgtaaa ataaaattgt agacaatatg
      241 cctaaaatcg ccgaatcaac cgcgaccaga gctgagcaag tgtttgcgca gttacaggcc
      301 gctatcattc gcggcgaatt ggctcctggt agcaaaatct ccgagcaaga tttggccgat
      361 acctatggca tcagccgtgg gccattgcgt gaggctttga atcgattgga aggacagaag
      421 ctgctggtgc gcatgccgca tgtgggcgca cgggtggtgt ctttgggcaa gcgcgaattg
      481 gaagagctgt acgatgtgcg cgccgaattg gaaggcatgg cctgtcgctt ggccgctgca
      541 cacatgagcc gggaagacgt tgatgctttg tatgcggtgt tgcaaacaca tgagcgagac
      601 ccgcagtttc aggcaggcac agcctatttc gagcaagacg gtgactatga ctttcattac
      661 cgcatcatca ctgccagtgg caacaatact ttgaaaaaac tgttgtgtga tgatttgtat
      721 caactggtgc gcatgtaccg cattcgtttt tccagtgtgc ccaagcgccc gcaaacggct
      781 ttaagcgaac actaccgcat tgttgatgcc ttggctgctg gtgacggtga attggctgaa
      841 ttgctgatgc gccgccacat acaggcatca aaacgcaata tggccagcca ttttgacgcg
      901 gcccaacttg aggaaacccc atgactcaca tcaccccagg ccaacgcttt cggagcgctg
      961 tggcagaaga gcagccctta caagtggtgg gcactttaac tgcctatacc gcgcggatgg
     1021 cggaagccgt gggctataag gctttgtaca tttcaggcgg tgcggtggcg gccaattctc
     1081 tgggcttgcc cgatttgggc atttccacca tggaagacgt gttaatcgat gccaatcgca
     1141 tcaccaatgc caccgaattg cccgtgttgg tggacataga cacgggttgg ggcggggcgt
     1201 ttaacattgc ccgcacggtg aaatcgttta ttaaagcagg cgtggccgcg gtgcacatgg
     1261 aagaccaaac gggcaccaag cgctgcgggc atcgccccaa caaggcggtg gtgagcaaag
     1321 aagagatgat agaccgcatc aaggccgcgg tggatgcacg caccgacgac agctttgtca
     1381 tcatggcgcg ttcggatgct ttggcttctg agggtttgga ccgcatgata gagcgttgcc
     1441 tggcctatgt ggaggcaggt gctgacatgg tgtttcctga ggcgattacc tcgctcaata
     1501 tgtacaaaaa agtgcgcgag gcggtgggcg taccgatttt ggcgaacatc accgaatttg
     1561 gccaaacacc gctgtttacc cgcgatgaat tggcctctgc caatgtggac atcat
//
LOCUS       DAOMVK010000525         1610 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_22230_length_1610_cov_3.276527, whole genome shotgun sequence.
ACCESSION   DAOMVK010000525 DAOMVK010000000
VERSION     DAOMVK010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22230_length_1610_cov_3.276527"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..>1610
                     /gene="acs"
                     /locus_tag="PK856_12385"
     CDS             2..>1610
                     /gene="acs"
                     /locus_tag="PK856_12385"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HRL03210.1"
                     /translation="MAAIESVLKENRVFEPSEDFKAHANVSGMEGYNALCEKANQDYE
                     GFWGELAQETITWKKPFTQVLDESRAPFYKWFADGVLNISYNSIDRHLADKANKTAII
                     YEKDDGSSENITYQELYERVCRFANGLKSLGVQKGDRVVIYMPMVPDAVVAMQACARI
                     GAIHSVVFGGFSSGAVKDRVQDAAAKVIITANESVRGGKSTPLKATVDEALKDGDCSS
                     VEKVVVFKRTETDVPFTAGRDIWWNDLIANQAAECEPEWVDAEHPLFILYTSGSTGKP
                     KGVQHSTGGYLLGAILSMKWVFDYKPDDIYWCTADVGWITGHTYIAYGPLAVGATQVV
                     FEGVPTYPDAGRFWQAIEKHKVTTFYTAPTAIRSLIKLGADLPKQYDLSSLRLLGTVG
                     EPINPEAWMWYYEVVGGSRCPIVDTWWQTETGSNMIAPLPGAVAIKPGSCTLPLPGIM
                     VDVVDESGASVDKSNGGFLVISRPFPSLLRTIWNDDARFQSTYFPEELGGHLYLAGDS
                     AHRDEKGYVWIMGRIDDVLNVSGHRLGT"
BASE COUNT          398 a          384 c          453 g          375 t
ORIGIN      
        1 tatggcagca atcgaatccg tattaaaaga aaaccgtgtg tttgagcctt cagaagattt
       61 caaagcgcat gccaacgtca gcggcatgga gggctataac gctttgtgtg aaaaagccaa
      121 tcaagattac gaaggatttt ggggcgaact ggcgcaagaa accatcactt ggaaaaaacc
      181 atttacccaa gttttggatg aaagccgcgc gccgttttac aaatggtttg ctgacggtgt
      241 gctgaatatt tcttacaaca gcatagaccg ccatttggct gacaaagcaa acaaaaccgc
      301 catcatttat gaaaaagacg atggcagttc agaaaacatt acttaccaag aattgtatga
      361 gcgcgtgtgc cgttttgcca atggtttgaa atcattgggc gttcaaaaag gcgaccgcgt
      421 ggtgatttac atgcccatgg tgcctgacgc ggtggtggcg atgcaagcgt gtgcgcgcat
      481 cggtgccatc cattcggtgg tgttcggtgg cttctcgtct ggcgcggtga aagaccgcgt
      541 gcaagacgcg gctgccaaag tcatcatcac cgccaacgaa agtgtgcgtg gtggaaaatc
      601 cacgccattg aaagccacgg tagacgaggc tttgaaagat ggcgattgca gcagtgtgga
      661 aaaagtggtg gtgttcaagc gcaccgaaac cgatgtgcca ttcaccgcag gccgcgacat
      721 ttggtggaac gatttgattg ccaatcaagc cgcagaatgt gagccagagt gggtagatgc
      781 agagcatccc ttgttcatct tgtacacttc aggcagcaca ggcaagccca aaggcgtgca
      841 gcacagcacg ggtggctatt tgctcggtgc catactgtcg atgaaatggg tattcgatta
      901 caaacctgac gacatttact ggtgtacggc cgacgtgggt tggattacag gccacactta
      961 cattgcttat ggcccattgg cagtcggagc tacccaagtg gtgtttgaag gcgtgccaac
     1021 gtatcccgat gcaggccgct tctggcaagc gattgagaag cataaagtca ctaccttcta
     1081 taccgcgcca accgcgatac gctcattgat taaattgggt gccgacttgc ccaaacaata
     1141 cgatttgtcg agcttgcgct tattgggcac agtgggtgag ccgattaatc cagaagcttg
     1201 gatgtggtat tacgaagtcg tgggtggcag ccgttgtccg attgtcgaca catggtggca
     1261 aaccgagact ggttccaaca tgatagcccc attgccaggc gcagtggcca tcaaaccagg
     1321 ctcgtgcacc ttgcctttgc caggcatcat ggtcgatgtg gtggatgaat caggtgcttc
     1381 ggtggacaag agcaacggtg gcttcttggt gatttcgcgc cccttcccat cgctgttgcg
     1441 cacgatttgg aacgacgatg ctcgcttcca atcgacttac ttccctgaag aattgggcgg
     1501 ccatttgtat ttggcaggcg attcggccca ccgcgatgaa aaaggctatg tgtggatcat
     1561 gggccgcatc gacgacgtgt taaacgtgtc gggccaccgc ttgggcacca
//
LOCUS       DAOMVK010000526         1609 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_22250_length_1609_cov_2.188546, whole genome shotgun sequence.
ACCESSION   DAOMVK010000526 DAOMVK010000000
VERSION     DAOMVK010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22250_length_1609_cov_2.188546"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1443)
                     /locus_tag="PK856_12390"
     CDS             complement(133..1443)
                     /locus_tag="PK856_12390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRL03211.1"
                     /translation="MLSSPALNEHIDSYYAASRHPLTQYPQLTGHIEVDTCIIGGGLA
                     GIGAALPLAKQGHSVAVLEAARIGFGASGRNGGQAITSYACEMEYLQEQLGLQDAQKL
                     WQWSIEAVNLIENQVKEYHIDCDWQRGYASVAIGNKKWDELQSYREYMRQTYGYEGYE
                     VWDRPKLKQHLKSDRYTGGIYDTDSGHVHPLNYCLGLAAAAATHGAQLFEQSFVSDIQ
                     PQAGKWRITTAQGDVLAKNVVLACNVFAGVLKQSSYMQSLADKIMPVGTYVIATEPLG
                     ERANDTINNNMAVCDTNFVLDYYRLSGDKRMLFGGKVSYSGQTPKDLANAMRSDMLRV
                     FPHLADIKIDYAWGGFVDITMNRAPHFGELAPGLMFLQGFSGHGVALTGLAGLVTAEK
                     ILGAPERFDLFSKLKHRSFPGGPYLRTPSLVLGMGWYRLKDYLK"
BASE COUNT          383 a          393 c          407 g          426 t
ORIGIN      
        1 tttaaatgcc aaagcaaata cttgctcatg gtcttcaaaa taaatgtgtg cgtggtaatc
       61 catttttata tgcgtcctgt aaatgcaaaa acggccacac aaaggcagcc gtttgagcgg
      121 ggtgtgtggg tgttatttta aataatcttt taagcgatac caacccatgc cgagcaccaa
      181 tgacggtgtg cgcaaataag gaccacctgg gaatgagcgg tgtttgagct tgctaaacaa
      241 gtcaaaccgc tctggtgcac caaggatttt ttctgccgtc accaagcctg ccaaacctgt
      301 taaggcaacg ccatggcctg agaagccttg taagaacatc aagccaggtg ccaactcacc
      361 aaagtgtggc gcacggttca tggtgatgtc gacaaagccg ccccaagcat aatcgatttt
      421 gatgtcggcc aaatggggaa acacgcgcaa catgtcgctg cgcatggcat tggccaagtc
      481 tttcggcgtt tggcctgaat agctgacttt gccgccaaac aacatgcgtt tgtcgccgct
      541 taagcggtag taatccagca caaaattggt gtcgcacacg gccatattgt tgttgatggt
      601 gtcgttggca cgctcgccca aaggttcggt ggcaatcaca tacgtgccga ctggcatgat
      661 tttgtctgcc aagctttgca tgtaggaaga ttgctttaac acgcctgcaa acacattgca
      721 ggccaagacc acatttttgg cgagcacatc gccttgcgcg gtggtgatgc gccatttgcc
      781 tgcttgtggc tggatgtcgc tgacaaacga ttgttcaaac aattgcgcgc catgagtggc
      841 tgctgcagcg gccaagccca aacagtaatt caatggatgc acatggcctg aatcggtatc
      901 ataaatgcct cctgtgtagc ggtcgctttt caaatgttgt ttgagtttgg gtctgtccca
      961 aacttcatag ccttcatagc cataggtttg gcgcatatac tcgcgataag attgcaattc
     1021 atcccatttc ttattgccta tggcaacgga tgcatagccg cgttgccaat cacaatctat
     1081 gtggtattcc ttgacttggt tttcaatcag attgaccgct tcgatagacc attgccacaa
     1141 cttttgggca tcttgtaagc ccaattgctc ttgcaaatac tccatttcgc acgcgtatga
     1201 ggtgatggct tggccaccat tgcgccccga cgcgccaaag ccaatgcgtg ctgcttccaa
     1261 taccgcaacg ctgtggcctt gtttggccaa aggcaaagcc gcgcctatgc ctgccaagcc
     1321 gccacctatg atgcaagtgt caacttcaat gtggcctgta agctgtgggt attgggtgag
     1381 gggatggcgg ctggccgcgt aataggaatc gatgtgctcg tttaatgcag gggaagacaa
     1441 catataggca atctccttgt agtaaaaagc cccgtagtac attgctacgg ggctgggtgt
     1501 gaatactata ctgcagtatt gttttcttgt gttgtacctt gtcggtaggc ttgtgccatt
     1561 tcccgttcgc gcttggtggt ttgtcgcatc atcagccaat tgagcaaga
//
LOCUS       DAOMVK010000527         1608 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_22266_length_1608_cov_3.306504, whole genome shotgun sequence.
ACCESSION   DAOMVK010000527 DAOMVK010000000
VERSION     DAOMVK010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22266_length_1608_cov_3.306504"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="PK856_12395"
     CDS             <1..788
                     /locus_tag="PK856_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRL03212.1"
                     /translation="IDGAVLTEDNIPVAEISGGCLCCVAGPQMTVTVTKMLRENRPDR
                     LIIEASGLAHAASVIDELNTPPLANALTVGAVLTVVDPRQFVDPDFNLQGLYQDQIGV
                     CDVLVASKVDLCSDEVMTQFRERAAKLFPPKALVAEVNNAQIDIAWLDTPVIQKPRYR
                     LHTLPDNTMGYQSQGFTFDKNSDFDGERLTQFFNDLPQLTEGLVRAKGVFRVLGTWVW
                     LNWVDGQWGANQVSWRRDSRFELIAKSFDADKIELALQAALER"
     gene            complement(835..1323)
                     /locus_tag="PK856_12400"
     CDS             complement(835..1323)
                     /locus_tag="PK856_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03213.1"
                     /translation="MKTILFLLLAALPAWAAAQTFLPLPENSDSLEKLNAMHSCSFHQ
                     ILPKQRFKQLRPEQLGPVVGVSVEMMDGKVFKLGKYFRPQQEVVFYADDEAFNVQDSA
                     LKSGLARAQHGADVLQLQYERDLSTAREIEEDMMFGIFNVTLQQHGKITHQQKLIRMC
                     QM"
     gene            complement(1359..>1608)
                     /locus_tag="PK856_12405"
     CDS             complement(1359..>1608)
                     /locus_tag="PK856_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRL03214.1"
                     /translation="PCGMVYSASLYALSSGSALQGGLSMAAFGLGTLPNLLAIGLFAS
                     QLTPLFQHVWLRRCIGGLMIAWAVWQLARVFGVLPAMH"
BASE COUNT          396 a          402 c          429 g          381 t
ORIGIN      
        1 gcattgatgg cgcggttttg accgaagaca acattcccgt ggccgaaatt tcaggtggct
       61 gtttgtgttg tgtggcgggc ccgcaaatga cagtgaccgt gaccaaaatg ttgcgcgaaa
      121 accgtccaga ccgtttaatc atcgaggcca gtggtttggc ccatgccgcc agcgtgattg
      181 atgaattgaa cacgcctcct ttggctaatg ccttgacagt gggcgcggta ttgaccgtgg
      241 tagacccgcg ccaatttgtc gatcccgatt tcaatttaca aggtttgtac caagaccaaa
      301 tcggcgtgtg cgacgtgttg gtggcgagca aagtggactt gtgcagcgat gaggtgatga
      361 cacagtttcg tgagcgcgcg gccaaattgt tcccacccaa agccttggtg gcagaggtga
      421 acaatgccca aatcgacatt gcttggctgg acacacctgt gattcaaaaa ccgcgttacc
      481 gcttacacac tttgcctgac aacaccatgg gctaccaatc gcaaggcttt accttcgata
      541 aaaacagcga tttcgatggc gaacgcctga cccaattctt caatgacttg ccccagttga
      601 ccgaaggctt ggtgcgcgcc aaaggcgtgt tccgcgtgtt gggcacttgg gtatggctca
      661 attgggtgga tggtcaatgg ggtgcgaacc aagtgtcgtg gcgccgtgat tcgcgttttg
      721 aattgattgc caaatcgttt gatgcagaca aaatcgaact agccttacag gccgcattag
      781 agcgttaata agcggcctgt aaaccctaac aaagcaagcc taggcttgct tttgtcacat
      841 ttggcacatg cgtatcagct tttgctggtg ggtgattttt ccatgctgtt gtaaggtgac
      901 attaaagatt ccgaacatca tgtcttcttc aatctcgcgt gcggtactca aatcgcgttc
      961 gtattgtaac tgcaagacat cagcgccatg ttgggcgcgt gccagcccag acttaagagc
     1021 agagtcttga acattgaacg cttcatcatc tgcataaaag accacttctt gctgcgggcg
     1081 aaaatacttg cccagtttga agactttccc gtccatcatt tcgacactga cgccgacaac
     1141 agggcccaat tgctcaggcc ttagctgctt gaagcgttgc ttgggtagga tttggtgaaa
     1201 agagcaagag tgcatggcat ttaacttttc caagctgtcg ctgttttcag gcaaggggag
     1261 gaaggtttgt gcggctgccc aagcgggcaa ggctgcaagc agcaggaata aaatggtttt
     1321 cataacatgg ctcactgagt ggagaacagc gtttcgcatc aatgcatggc aggcaacacg
     1381 ccaaataccc gtgccaattg ccatacggcc caagcaatca ttaaaccacc gatgcagcgt
     1441 ctgagccaga catgttgaaa cagcggcgtc aattggctag caaacaaacc gatggccagc
     1501 aaattgggca aggtgcccaa gccaaacgct gccatgctca agccaccttg taaggcgctg
     1561 ccgctgctta aggcatagag cgaggcgctg tacaccatgc cacacggc
//
LOCUS       DAOMVK010000528         1606 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_22310_length_1606_cov_2.286267, whole genome shotgun sequence.
ACCESSION   DAOMVK010000528 DAOMVK010000000
VERSION     DAOMVK010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22310_length_1606_cov_2.286267"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PK856_12410"
     CDS             complement(<1..192)
                     /locus_tag="PK856_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03215.1"
                     /translation="MATDYQRALHTLRHWIDSDVEQQRFAVRDLSEMEWAAIAEATDM
                     RLPAAYYEFIRQIGVGEFFL"
     gene            complement(287..>1606)
                     /locus_tag="PK856_12415"
     CDS             complement(287..>1606)
                     /locus_tag="PK856_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004286167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRL03216.1"
                     /translation="KSAEFIEIIAKRIEQEGIEAWFKLDKADLLDASEVDAYDKLPDT
                     LDVWFDSGSTHFAVLKQREELKWPADLYLEGSDQHRGWFQSSLLTACATGGRAPYNQL
                     LTHGFVVDGKGQKMSKSIGNVIAPQKVNDSLGADILRLWVASTDYSGELAISDEILKR
                     TTDSYRRLRNTIRFLLANLSDFDPITDAVAYNDMLPLDRYAVVLARQLQERLVGDHYP
                     RYAFHYAVQDIVHFCADEMGAFYLDILKDRLYTMKADSRGRRSAQTALYHITRSLILG
                     ISPILCFTAEEAWEILAGGNEDSVLYHTLHEFPSMPETVEAELAKKWQAVREVREAVN
                     KEIETKRAAEVVGSSWQAAVSITAPEPTYGYLRALGEELRFVLMVSKVGVTEGEALGV
                     VVDKAEGEKCERCWHYLPDVGSNTAHPCLCVRCIDNIEGKGEERLLA"
BASE COUNT          392 a          444 c          385 g          385 t
ORIGIN      
        1 caggaaaaac tcgcccacac cgatttgacg gatgaactca taataggcgg caggcaaacg
       61 catgtctgtg gcctcggcta ttgcagccca ttccatttca ctgaggtcac gcactgcaaa
      121 acgctgctgc tcgacatcgc tgtctatcca atgccgcaaa gtgtgcagcg cccgttggta
      181 atctgttgcc atattgctcg tcctcaaaat gtgtacacat gatagcaaaa gccataaaaa
      241 agcggcctgt aaggtttaca ggccgcttgt tcaaggtttc atgcgattaa gccaacaaac
      301 gctcttcgcc tttgccttcg atgttgtcga tgcagcgcac gcacaaacat gggtgagctg
      361 tgttgctgcc cacgtctggc aagtaatgcc agcaacgctc gcatttttcg ccttcggctt
      421 tatcaacaac gacacccaag gcctcgcctt cggtaacgcc gactttcgac accatcaaca
      481 caaagcgcaa ttcttcgccc aaagcacgca aatagccgta agttggctca ggtgcggtaa
      541 tgctcactgc cgcttgccaa gatgaaccca ccacttcggc cgcacgcttg gtttcgattt
      601 ctttgtttac cgcttcgcgc acttcgcgca ccgcttgcca ttttttcgcc aattccgctt
      661 ccacggtttc aggcatggat gggaattcgt gcaaggtatg gtagagcact gagtcttcgt
      721 tgccgcctgc caagatttcc cacgcctctt ctgcggtgaa gcacaagatg gggctgatgc
      781 ccaaaatcag gctgcgggtg atgtggtaca acgcggtttg tgcgctgcga cggccacggc
      841 tgtcggcttt catggtgtag aggcggtctt ttaagatgtc taaatagaac gcgcccatct
      901 cgtcggcaca gaaatgcaca atgtcttgca ccgcatagtg gaaggcataa cgcggataat
      961 ggtcgcccac caaacgctct tgtaattgac gcgccaacac caccgcataa cggtcgagtg
     1021 gcaacatgtc gttataggcc acggcatcgg taatcgggtc gaaatccgac aagttggcca
     1081 acaagaaacg gatggtattg cgcaaacggc ggtagctgtc ggtggtacgc ttcaagattt
     1141 cgtccgaaat cgccaattca cctgagtaat cggtagaagc cacccacaaa cgcaagatgt
     1201 ccgcacccaa agagtcgttc actttttgtg gcgcaatcac attgccgatg gatttggaca
     1261 ttttttgtcc tttgccatcc accacaaaac catgggtcaa caattggttg taaggcgcac
     1321 ggccaccggt ggcgcaagcc gtcaacaatg aagattggaa ccaaccacgg tgttggtctg
     1381 aaccttccaa atacaaatcg gcaggccatt tcaactcttc gcgctgtttc aataccgcaa
     1441 aatgggtaga gccagaatca aaccacacgt ctaaggtgtc aggcaatttg tcgtaagcat
     1501 cgacttcgct tgcgtccaac aaatcggctt tgtccaattt aaaccaagct tcgatgcctt
     1561 cttgctcaat gcgtttggca atgatttcga tgaattccgc actctt
//
LOCUS       DAOMVK010000529         1604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_22348_length_1604_cov_3.593286, whole genome shotgun sequence.
ACCESSION   DAOMVK010000529 DAOMVK010000000
VERSION     DAOMVK010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22348_length_1604_cov_3.593286"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PK856_12420"
     CDS             <1..358
                     /locus_tag="PK856_12420"
                     /inference="COORDINATES: protein motif:HMM:NF013089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HRL03217.1"
                     /translation="KMIYWLFLAASIALEVVGVLLLNHASQTNATSTMMVMYALVIAS
                     YFLLSKAVKRIPLGIAYALWEGAGIVLITASSVVLFGEHISAMKIVGLSLLLVAITLL
                     KSGTQTAAPALHIKEA"
     gene            359..691
                     /gene="mdtI"
                     /locus_tag="PK856_12425"
     CDS             359..691
                     /gene="mdtI"
                     /locus_tag="PK856_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HRL03218.1"
                     /translation="MLEHFSWIHGLWLFGAIALEILANVLLKMSNGFKRISLGAAAIA
                     CVLGAFTCLSFAVKGIPLSVAYALWGSFGIIITVIMGMVLFQQRLNRKGWLGLSMLVL
                     SIVLIKMA"
     gene            complement(810..>1604)
                     /locus_tag="PK856_12430"
     CDS             complement(810..>1604)
                     /locus_tag="PK856_12430"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRL03219.1"
                     /translation="AVSKQVAMLEYTLKIQLFIRIRQRLQLSPPGQLYLQEVTHILNL
                     IEDASLEMLSYGNRTESIRIATHPSFGTNWLIPTLQGFAQQYPHLELVFQDYVEPTEL
                     NDTDVDVAFIHGNGHWDKLTTIKLFDEIMVPVCSAGLWVRYQQEESNSIYFNEPLLHC
                     RTRVGAWPEFFSRFEIEHPNGYRGTRFETWTSVLAAAEHDFGIALVPEFLAHNLLLTG
                     RLIRLGTQQLDESGAYYIAYPEHYSAEPKIVALSEWITERTLEKNA"
BASE COUNT          382 a          389 c          399 g          434 t
ORIGIN      
        1 aaaaatgatt tactggttgt ttttagcagc gtcgattgcg ctggaagttg tgggtgtttt
       61 actgttaaac cacgcctctc aaaccaatgc cacctctacc atgatggtga tgtatgcctt
      121 ggtgattgcc tcttattttc tgttgtcaaa agccgtgaaa cgcatccctt tgggcattgc
      181 ctatgctttg tgggaaggcg caggcatcgt gttgattact gcgtccagcg tggttttgtt
      241 tggcgaacac atttctgcca tgaaaatcgt cggcttgagc ctgttgctcg tggccatcac
      301 cttattgaaa tcgggaaccc aaaccgccgc ccctgccttg cacatcaagg aggcgtaaat
      361 gttggaacac tttagctgga tacatggctt atggctgttt ggcgccatcg ccttggagat
      421 tttggccaac gtcttgttga aaatgtcgaa tggctttaag cgcatcagtt tgggcgcggc
      481 agccattgcc tgtgtattgg gcgcgtttac ctgcttgtct ttcgccgtga aaggcattcc
      541 cttatctgtg gcctatgctt tgtggggcag ctttggcatc atcatcaccg tcatcatggg
      601 catggtattg tttcagcagc gcctcaaccg caaaggctgg ttaggcctga gcatgttggt
      661 attgagcatc gtactcatca aaatggctta agccaaacaa acacaataaa gcggcctgta
      721 aaccttgctt aagggtttac aggccgcttt attatggcat cagtactgat agcaatggct
      781 aatccaatac actcatgttg atggtagggt catgcgtttt tttctaaggt gcgctcggtt
      841 atccactcgc tcaaggcaac gattttcggc tcagcggaat aatgttctgg ataagcaatg
      901 taatacgcgc ctgattcatc caattgctgt gtgcccaagc gtatcaagcg gcctgtaagc
      961 aacaaattgt gcgccaaaaa ttcgggcacc aaagcaatgc caaaatcatg ctcggccgcc
     1021 gccaacaccg atgtccacgt ttcaaaacgc gtgccacgat agccattggg gtgttcgatt
     1081 tcaaagcggc tgaaaaactc aggccatgcg cccacacggg tgcgacaatg cagcaaaggc
     1141 tcgttgaaat aaatgctgtt gctctcttct tgctgatagc gtacccacaa gcctgccgaa
     1201 cacacaggca ccatgatttc gtcaaacagc ttgatggtgg tgagcttgtc ccagtgccca
     1261 ttgccgtgaa taaacgccac gtccacatcg gtatcgttca actcggtggg ctctacatag
     1321 tcttgaaaca ccaattccaa atgcgggtat tgctgcgcaa aaccttgcaa agttggaatc
     1381 aaccagttgg tgccaaagct tggatgggta gcgatgcgta tgctttcggt gcgattgcca
     1441 tacgacaaca tttccaaaga agcgtcttcg attaaattca aaatgtgggt gacttcttgc
     1501 agatacaact gcccaggagg cgacaattgc aggcgctggc ggatgcggat gaacaattgg
     1561 atttttaagg tgtattccaa catcgccact tgcttgctca cggc
//
LOCUS       DAOMVK010000530         1604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_22352_length_1604_cov_1.954164, whole genome shotgun sequence.
ACCESSION   DAOMVK010000530 DAOMVK010000000
VERSION     DAOMVK010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22352_length_1604_cov_1.954164"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            605..826
                     /locus_tag="PK856_12435"
     CDS             605..826
                     /locus_tag="PK856_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03220.1"
                     /translation="MFTQMHIAKVDAIEAKIEMELMFLSEANKLDCLELIMGLDAEDR
                     IAYLNQYVSKMAALMGTEAVADVAWLMAA"
     gene            complement(1189..>1604)
                     /locus_tag="PK856_12440"
     CDS             complement(1189..>1604)
                     /locus_tag="PK856_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HRL03221.1"
                     /translation="FALFFFAFTTIVAYYYIAETNIAYLTRKNEKMRSTLMLVLKLAM
                     IVMVVFGCMRTAELAWGLGDMGVGLMAWLNIIAILLLQKPALQSLKDYERQQKEGKDP
                     IYVPEDNGVHNAPIWKEIGEQFKKMDTPVKDRFIP"
BASE COUNT          419 a          290 c          442 g          453 t
ORIGIN      
        1 gcttggaatt gatcatgggc ttggatgccg aagaccgcat cgtctactta aaccaatacg
       61 tgagcaaaat ggccgcgttg atgggcactg aagccgtagt ggcagacgtg gcttggttga
      121 tggctgctta agtttgctgt aaaagctgtt ttcagtgtgt tgtttttgaa tcgaacacaa
      181 acaaatcgcc cttggtaaat gccaagggcg atttgctgta tgggttttgg tggtatcggc
      241 tttgggtgag gtaatttgtg gcagattacg gcagctgagt gcagtacatg accatgtaag
      301 tgttgtatgt ggccataggg tggcaataaa tatgaaaaac tggccgcaaa tgggtacatg
      361 gtatggatgt gtgatggttt gagcaggtaa ggctgtgact gattgaagga tacaggaatt
      421 gagcggtgag tttttatgcg tctgtgcttt ttaaaagaat ataggctagg cgggggattg
      481 cagatgttgt ggcttgattt ataagataaa agctttaatt tactttgggt tgaatatcaa
      541 tttggcatgg tggttgctca gtattgatta cattcaaaaa cacactgatt taaggattac
      601 cgccatgttt acccaaatgc acattgccaa agttgacgcc atcgaagcca aaatcgaaat
      661 ggaattgatg ttcttgtctg aagccaacaa attagactgc ttggaattga ttatgggctt
      721 ggatgccgaa gaccgcatcg cttacttaaa ccaatacgtg agcaaaatgg ccgcgttgat
      781 gggcactgaa gccgtggcag acgtggcttg gttgatggct gcttaagttt gcagtaaagc
      841 tgttttcagt gtgttgtttt tgaatcgaac acaaacaaat cgcccttggc aaatgccaag
      901 ggttttttgt agctggcggt gtatcaggtg aagaggtttg tgttaagggc atatgggtga
      961 ggatgaagcg cgttgatttg ttgcccaata gggcgtgttg ttggcgccat tgcctgtgag
     1021 cagcgagggg ttgtgcttgc tctatttcta atgggtagag aagatggata gagaaacagg
     1081 gtgagggcgg cgcaagtgac cataaaagcg gcctgtaacg gcaaacctca tggcttacag
     1141 gcaaaaaaaa accttggtag cgaccaaggg ttttttaatg ctaaaggatt acggaatgaa
     1201 gcggtctttt acgggcgtat ccattttctt gaattgctca ccgatttctt tccaaattgg
     1261 cgcgttgtgc acgccattgt cttcaggcac ataaattggg tctttgcctt ctttttgttg
     1321 gcgctcgtag tctttaagcg attgcagtgc gggtttttgc aacaacaaga tggcgatgat
     1381 gttcagccaa gccatcaagc ccacgcccat gtcacccaag ccccaagcca attcagcagt
     1441 gcgcatgcaa ccgaatacca ccatcactat catcgccaat ttcaatacca acatcaaagt
     1501 agagcgcatt ttttcatttt tgcgggtcaa gtaagcgatg ttggtttctg cgatgtagta
     1561 ataggccacg atggtggtga aagcgaagaa gaacaaggca aaag
//
LOCUS       DAOMVK010000531         1599 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_22474_length_1599_cov_1.878238, whole genome shotgun sequence.
ACCESSION   DAOMVK010000531 DAOMVK010000000
VERSION     DAOMVK010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22474_length_1599_cov_1.878238"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PK856_12445"
     CDS             <1..486
                     /locus_tag="PK856_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRL03222.1"
                     /translation="VVLFNGTIMDNIAYGELSHHSREDVERAAKMANAWQFIEKLPQG
                     LDTLIGENGTKLSGGQRQRIAIARALLKNAPILILDEATSALDNESERLVQAALEQLM
                     QARTTIVIAHRLSTIEQADLIVVMDNGQVVEQGTHSELIQKQGRYAHLHSAQLIKHSP
                     E"
     gene            513..1289
                     /locus_tag="PK856_12450"
     CDS             513..1289
                     /locus_tag="PK856_12450"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HRL03223.1"
                     /translation="MAAFNSMKVLSVHHWTEAYFSFTCERDESLRFENGQFVMVGLMV
                     DGKPLMRAYSVASANWEEHLEFFSIKVQNGPLTSRLQHLQVGDEVLISKKPTGTLIAS
                     DLNAGKNLYLLSTGTGLAPFLSVTKDPDIYEQFEKVILVHGVRYKKDLAYFDHFTKEL
                     QEHEYLGDLVKEKLIYYPIVSREEFPHQGHMTDLMKSGKLFEDIGLPFMSAENDRAML
                     CGGPGMLKDVSAMLDEFGLQVSPKTGVRGDYVIERAFVEK"
     gene            1363..>1599
                     /locus_tag="PK856_12455"
     CDS             1363..>1599
                     /locus_tag="PK856_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03224.1"
                     /translation="MLDLVLWILLPLCVGFAVRISNSKQLHLINESLTVIVYLILLLI
                     GMELAQVDNLGRELGFILGVSLLFLVVNVGCNILA"
BASE COUNT          426 a          338 c          423 g          412 t
ORIGIN      
        1 gtggtgttgt tcaatggcac catcatggac aacattgcct atggtgaatt gagccatcac
       61 agccgtgaag atgtcgaacg tgcggccaaa atggccaatg cgtggcaatt cattgaaaaa
      121 ttgccacaag gtttggatac cttaatcggc gagaacggca ccaaattgtc gggtggacaa
      181 cgccagcgca tcgccatcgc gcgtgcgctg ctcaaaaatg ccccgatttt gattttagat
      241 gaggcgacca gtgctttgga caatgagtct gagcgtttgg tacaggccgc cttagagcag
      301 ttaatgcaag cgcgcaccac cattgtgatt gcccaccgtt tgtctaccat cgagcaagcc
      361 gatttgatcg tggtgatgga caacgggcaa gtggtggaac aaggcaccca cagtgagtta
      421 atccaaaaac agggtcgtta tgcgcatttg cactcggcac aactgattaa acattcaccc
      481 gagtgataga ttgatattta aggaaataaa acatggcagc atttaacagc atgaaagtct
      541 tgtctgtaca ccattggaca gaggcgtatt tttcattcac ttgtgagcgt gatgagagtt
      601 tgcgttttga aaacggccaa ttcgtgatgg ttggcttgat ggtagatgga aaacctttga
      661 tgcgtgccta cagtgtggct tcagccaatt gggaagagca tttggaattt ttcagcatca
      721 aagtgcaaaa tggcccattg accagccgct tgcagcattt gcaagtgggc gatgaagtgt
      781 tgatcagcaa aaagccaaca ggcacattga tcgccagcga tttgaatgcg ggcaaaaacc
      841 tgtatttgtt gtcaactggc acaggtttgg caccgttctt gagcgtgacc aaagaccctg
      901 atatttatga gcagtttgaa aaagtgattt tggtacacgg tgtgcgctac aaaaaagact
      961 tggcgtattt cgatcatttc accaaagaat tgcaagaaca cgaatacttg ggcgacttgg
     1021 tgaaagaaaa attgatttac tatcccatcg tgtcgcgtga agaattcccg caccaaggcc
     1081 acatgaccga tttgatgaaa tcaggcaaat tgtttgaaga catcggcttg ccattcatga
     1141 gcgcagaaaa tgaccgcgcc atgttgtgtg gcggccctgg catgttgaaa gacgtcagcg
     1201 ccatgttgga tgaatttggc ttgcaagtgt caccaaaaac aggtgtgcgc ggcgattacg
     1261 tgattgagcg cgcgtttgta gagaaataag cctcaaaaaa cagtcgtatc acaaaacaac
     1321 aagcgggttt ggtttacagg ccggctttaa aaggacgagc agatgttgga tttggtgttg
     1381 tggattttat tgcctttgtg cgtgggcttt gccgtgcgca tcagcaacag caagcaattg
     1441 cacctcatca atgaatccct caccgtcatc gtctacctca tcttgttgtt gataggcatg
     1501 gagctggcac aagtagacaa cctcgggcgt gagctcgggt ttatcttggg tgtcagcttg
     1561 ttgtttttgg tggtcaacgt cggttgcaac atcttggcg
//
LOCUS       DAOMVK010000532         1596 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_22534_length_1596_cov_2.027255, whole genome shotgun sequence.
ACCESSION   DAOMVK010000532 DAOMVK010000000
VERSION     DAOMVK010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22534_length_1596_cov_2.027255"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..158
                     /gene="recA"
                     /locus_tag="PK856_12460"
     CDS             <1..158
                     /gene="recA"
                     /locus_tag="PK856_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="catalyzes the hydrolysis of ATP in the presence of
                     single-stranded DNA, the ATP-dependent uptake of
                     single-stranded DNA by duplex DNA, and the ATP-dependent
                     hybridization of homologous single-stranded DNAs; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRL03225.1"
                     /translation="QGKDNTRMWLKENPEIANEIEQKIRAEVGINAEITEGTLDETDG
                     DAPTDFE"
     gene            308..1345
                     /locus_tag="PK856_12465"
     CDS             308..1345
                     /locus_tag="PK856_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003773782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03226.1"
                     /translation="MFNKAQNTSNDNSVSHTIKQPPRLFPETQQAIANIEAYLGAPLV
                     CYWNSPRGSICGNDVLALYHLLEHIGNHDKIYVFIKSDGGSGIQALRMINLIRGHSKE
                     LVSLVPLECASAATMMAIGANQIHMGPMAYLSAVDTSLTHDLSPLDRDNDRVSVSLNE
                     LTRVVKLWRNNTEDKDSNPYKSLFEHVHPLVIGAVDRAESLSIMLCEELLSYHIPDKN
                     KVKQIAETLNNKYPSHSYPIMTEEARSIGLNIERMDTVLNNMLLDLNSLYAEMGQRAV
                     TDTDDTHSHSNEILNILETRGRQVFYQNDKDWFYRTEERRWLTLNDNSAWHNTEWNAQ
                     GQIHESIVHLA"
     assembly_gap    1348..1357
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          477 a          401 c          330 g          378 t
ORIGIN      
        1 gccaaggcaa agacaacacg cgcatgtggt tgaaagaaaa ccctgaaatt gccaacgaaa
       61 tcgagcaaaa aattcgtgcg gaagtgggca tcaatgctga aatcactgaa ggcactttgg
      121 acgaaaccga tggcgatgca cccaccgatt ttgaataaaa ccatctgaac atgatattca
      181 gcggcctgta agcatttaca ggccgctttc ttttgcttac aaaccattaa acacaagcta
      241 ttgcaggctc taagtattta tgttttgatg aatgctcatg tttcaacagc ccttatttgg
      301 agtgttcatg ttcaataaag cccaaaacac cagcaatgac aacagtgtgt cccacaccat
      361 caagcaacca ccacgtttgt ttcctgaaac ccaacaagcc attgccaata ttgaagccta
      421 tttgggcgcc cctttggtgt gttattggaa cagtccacgc ggcagcattt gtggcaacga
      481 tgtgctggcg ctttatcact tgcttgaaca tatcggcaac cacgacaaaa tttatgtgtt
      541 catcaagagt gacggcggta gtggcatcca agccttgcgc atgattaatt tgatacgtgg
      601 tcacagcaaa gaattggtgt cgctggttcc attggaatgt gcatccgccg ccaccatgat
      661 ggccataggt gctaaccaaa ttcacatggg tcccatggcg tatttgagcg cggtagacac
      721 ctcactcacc cacgatttgt caccactcga ccgcgacaac gaccgcgttt cagtgagctt
      781 gaacgagtta acccgtgtgg tcaaactctg gcgcaacaat accgaagaca aagattccaa
      841 tccgtataaa tcactgtttg agcacgtgca tcccttggtg ataggcgcag tagaccgcgc
      901 agaatccttg tccatcatgt tgtgtgaaga attgctgtct taccacatcc ccgacaaaaa
      961 caaggtcaag caaatcgctg aaaccctcaa taacaaatac ccatcgcact cctaccccat
     1021 catgaccgaa gaggcgcgct ctatcggcct gaacattgag cgtatggaca cggtattgaa
     1081 caatatgttg ctggatttga attctttgta cgccgaaatg ggccaacgtg cggtcaccga
     1141 taccgatgac acccattcac actccaatga aatcttaaac attttggaaa cccgcggtcg
     1201 ccaagtcttt tatcaaaacg acaaagactg gttttaccgt accgaagagc gccgttggct
     1261 caccttgaac gacaactctg cttggcacaa cactgagtgg aatgcccaag gccaaataca
     1321 cgaatccatt gttcacttgg catagccnnn nnnnnnngta acaaccaatt tacaggccgc
     1381 ttgttacata caagcggtct gtaaacgttt aaatgcacat tattgcttac ttgccataag
     1441 cacccaccag tacagataag atggatggca ggcatacgaa actgcagtgc ttcaatctaa
     1501 tatgtcaata taaaaaagat agagataaaa gaacaggccg cttacattga taagcagcct
     1561 gtaaatccat catcacatta cacaataaca acgcat
//
LOCUS       DAOMVK010000533         1586 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_22697_length_1586_cov_2.565643, whole genome shotgun sequence.
ACCESSION   DAOMVK010000533 DAOMVK010000000
VERSION     DAOMVK010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22697_length_1586_cov_2.565643"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(401..772)
                     /locus_tag="PK856_12470"
     CDS             complement(401..772)
                     /locus_tag="PK856_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03227.1"
                     /translation="MWHSYKQGIWALALAPTLAWASPIGEISDVKIKAPSTKVEAQCR
                     DFKPNKAQVQAFFKRAILITPRQEHDYFLYGPCTAEGTFKTRYGHWEWKMRNGGTAYV
                     EDVMGNLYTFADPKQESGLDE"
     gene            complement(819..>1586)
                     /locus_tag="PK856_12475"
     CDS             complement(819..>1586)
                     /locus_tag="PK856_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03228.1"
                     /translation="GGGDEPEHVRLALHEAVNDMNWRPNSKKVVFLVGDAPPKDSYIE
                     TPSVSHTAQKAKQKGIIINTLLAGDSKSTAKVWQSVAQYGGGEYFTIPQDGGSVALTT
                     PYDDKLSTLSNQLDQVYLPYGDVSVRRAAVAKSASKLSSISAAPKEALAERSINKAIN
                     TQAYSQDDLVQSIENGKVKLDAIDSKKLPDNMQNMSATERKAYVEGLVQKRQQLKQEI
                     ATTSKQREDYLNNNRQTLSSGDSFDSAVSRALSKQLH"
BASE COUNT          347 a          369 c          415 g          455 t
ORIGIN      
        1 ggtgttccaa tcccaaggct tgggccacca caagggcaat gaaggcgaca atcgtggttt
       61 tgaatgcaaa cgccagacgc ttgttatcaa agccgaatcg ttcgagtaag ggcgtgagag
      121 ggaatgaagc tgaaaacatg aacagacgat gggctaaagt gttgatgtta acattcggaa
      181 atgctgagta atagcccgtt taacggaata gatgctatta atgtttttaa taataatcag
      241 ctgatgagct tgtttgaagc gcggtgtgtg ttcaaacagc ttggtttcac agactgtcat
      301 gatttacagg ctgcttgttt caagccctgc atggcaatgt gaaacaagcc gctgaataac
      361 agcggcctgt aaggtgagac gattaatcaa tgtgacagga ttattcatcc aagcctgact
      421 cttgcttggg gtcggcaaaa gtatagaggt tgcccatcac atcttccaca taggctgtgc
      481 ccccattgcg cattttccat tcccaatggc cgtaacgcgt tttgaacgtg ccttcggctg
      541 tgcaagggcc atacaggaag taatcgtgtt cttgtcgcgg cgtaatcaaa atggcgcgtt
      601 taaaaaaagc ttgcacttgc gccttgttcg gtttgaaatc gcgacactga gcctcgactt
      661 tggtcgaggg cgctttaatt ttcacatcgc tgatttcacc tatgggcgag gcccaagcca
      721 gagtgggcgc aagtgccagt gcccaaatgc cttgtttgta actatgccac atgatggttc
      781 tcctgcatgt ctgtggtgcg atggctaatt gtccttgctt aatgcaattg tttgctcagg
      841 gcacgactca ctgccgaatc gaaactgtca ccgctgctta aggtttggcg gttgttgttc
      901 aaatagtctt cgcgctgttt ggacgtggtg gcgatttcct gcttcaattg ttggcgtttt
      961 tgaaccaaac cttccacata ggctttgcgt tcggttgccg acatgttttg catgttatca
     1021 ggcagtttct tgctgtctat cgcatccagt ttgactttgc cattttctat cgattgcacc
     1081 aaatcgtctt ggctgtaggc ttgggtgttg atggctttgt taatcgaacg ctcggccagt
     1141 gcttctttgg gcgctgcaga aatcgatgag agcttactgg cgcttttcgc aacggccgca
     1201 cggcgaacgc tgacatcgcc ataaggcagg taaacctggt caagctggtt gcttaaagtg
     1261 gacaatttgt catcgtatgg cgtggtcagt gcaacactgc caccgtcttg tgggatggtg
     1321 aaatactcac cgccaccgta ttgcgccacg ctttgccaca ctttggcggt ggatttgctg
     1381 tcacctgcca gcaaggtgtt gatgatgatg cctttttgtt tggctttttg agcggtgtgg
     1441 ctgacgctag gtgtttcaat atagctgtct ttgggcggcg catcacccac caaaaacacc
     1501 acttttttgc tgttgggccg ccaattcatg tcgttcactg cttcatgcaa ggccaaacgc
     1561 acgtgttcgg gctcatcgcc gccacc
//
LOCUS       DAOMVK010000534         1585 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_22714_length_1585_cov_2.654248, whole genome shotgun sequence.
ACCESSION   DAOMVK010000534 DAOMVK010000000
VERSION     DAOMVK010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22714_length_1585_cov_2.654248"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="PK856_12480"
     CDS             complement(<1..859)
                     /locus_tag="PK856_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HRL03229.1"
                     /translation="MTTQTQLPSLITIENGQKAKPTFSLAEYQRRQGLLRQKMAEMQV
                     DGVLFSSYHNINYYGDFLYCSFGRFYGLVVTQEKVVSISANIDGGQPWRRTVGDYNVV
                     YTDWQRDNYFKACAQELPKKGRVGIEMDNLPLERFAKLQAAMPQIEFVDISNECMRLR
                     MIKSAEEIEFIKNGANVCDIGGFALRDAVKEGVPEHEVALASTQAMIREIARRYPDSE
                     LMDTWTWFQSGINTDGAHNPVTTRKVQKGDILSLNCFSMISGYYTALERTMFLDHVDD
                     ASLKLWEVNV"
     gene            1020..>1585
                     /locus_tag="PK856_12485"
     CDS             1020..>1585
                     /locus_tag="PK856_12485"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRL03230.1"
                     /translation="MTTPTQTQHIGFVLVDGFSMIAFSNAVEAFRMANYVTKKPLYRC
                     SVAGLVNGHTAASNHISINHTAQMASLMDCDLIFVCGGFDLAKFNSSNLRHWLQRYAQ
                     QGINLGSTCTAAYALADAGLMDDYQASIHWENMLAAKEAFPQVQFNTTIYTIDRDRYT
                     CSGGAASLDLCINLIRLHHDRKLSEAIA"
BASE COUNT          384 a          393 c          384 g          424 t
ORIGIN      
        1 tcacgttcac ttcccacaat ttcaaagaag catcgtcgac gtggtctaag aacatggtgc
       61 gctctaaggc ggtgtaataa cctgaaatca tcgagaagca gttcaggctc aagatatcgc
      121 ctttttgcac tttgcgtgtg gtcactgggt tgtgtgcgcc gtcggtgttg atgcccgatt
      181 ggaaccaagt ccaagtgtcc atcaattcgc tgtcaggata gcggcgggcg atttcgcgta
      241 tcatcgcttg ggtagaggcc aaagccactt catgctcagg cacgccttct tttactgcgt
      301 cgcgtaaggc aaagccaccg atgtcgcaga cattggcacc gtttttaatg aattcgattt
      361 cttccgccga tttgatcatg cgcaaacgca tgcattcgtt gctgatgtca acaaactcaa
      421 tttgaggcat agcggcctgt aatttggcga agcgttccaa tggcagattg tccatttcaa
      481 tgccaacacg gcctttttta ggcaattctt gtgcgcaggc tttgaaataa ttgtcgcgtt
      541 gccaatcggt atagaccacg ttgtaatcgc ctacggtacg gcgccatggt tggccaccgt
      601 caatgttggc agaaatcgaa acgactttct cttgcgtgac aaccaagccg tagaaacggc
      661 cgaatgagca atacaaaaag tcgccataat aattgatgtt gtggtatgag ctgaacaaca
      721 cgccgtctac ttgcatttcc gccatttttt ggcgcaacaa accttggcgg cgttggtatt
      781 cggccaagct gaaagtaggc ttggcttttt ggccgttctc aatcgtgatc aaacttggca
      841 attgtgtttg agtcgtcatg gtgtttctcc ttgtagattt ttagagtcgt tgtgaattga
      901 caatgtgttg atgcttactc atcatctgag gcacatcata aaaagcacag gcggcctgaa
      961 atgtgtccaa tgcgacataa ttcagtctga tttggcaagc tacctaggag aaccccacta
     1021 tgactacgcc cacccaaacc caacacattg gatttgtatt ggtggatggc ttttcaatga
     1081 ttgccttttc caatgctgtg gaagcatttc gcatggccaa ttatgtgacg aaaaaaccgc
     1141 tgtaccgctg ctcggtagcg ggattggtga acggccatac ggccgccagc aaccacattt
     1201 ccatcaacca cactgcgcaa atggccagct tgatggactg tgatttgatt tttgtctgtg
     1261 gcggctttga tttggccaaa ttcaacagca gcaatttgcg ccactggtta cagcgctatg
     1321 cccagcaagg gatcaacttg ggcagcactt gtaccgctgc ttatgctttg gcagatgcag
     1381 gcttgatgga cgattaccaa gccagcatcc attgggaaaa catgttggcg gcaaaagagg
     1441 ccttcccaca agtgcagttc aacaccacaa tttacaccat agaccgtgac cgttacacct
     1501 gttctggcgg cgccgcttcg ctggatttgt gcatcaacct catccgttta caccacgacc
     1561 gtaagctgtc cgaagccata gccga
//
LOCUS       DAOMVK010000535         1584 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_22737_length_1584_cov_2.389797, whole genome shotgun sequence.
ACCESSION   DAOMVK010000535 DAOMVK010000000
VERSION     DAOMVK010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22737_length_1584_cov_2.389797"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1584)
                     /locus_tag="PK856_12490"
     CDS             complement(<1..>1584)
                     /locus_tag="PK856_12490"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HRL03231.1"
                     /translation="KDTTAYAIVNGEVEATLQNGSKVSLQNPALFVAYTGDTAAPASL
                     LFLHNGLHFDVVIDKTSAIGAQDPAGIKDVIMEAALSTIMDCEDSVAAVDGEDKTLVY
                     QNWFGLMQGSLAEEISKGGKTFTRRMNADRVYTGKDGQAITLPGRSLLFIRNVGHLMT
                     TPAILDKNGNEIPEGIMDCVMTALIAHYDLSRGENTNSRTGSVYIVKPKMHGPEEVAF
                     ANRLFGAAEQLVSLPANTLKMGIMDEERRTSANLKACIAQAKDRVVFINTGFLDRTGD
                     EMHTSMYQGAVLRKGDIKNTGWIKAYETRNVQIGLACGLAGKAQIGKGMWAMPDLMAE
                     MLEQKIGHPKSGATTAWVPSPTGAALHALHYHQVNVFDVQKQILAAPQASLLNELLTP
                     PLAENTNWSAADKQQELDNNCQGILGYVVRWVEHGVGCSKVPDIHNIGLMEDRATLRI
                     SSQHIANWLVHGVVTEAEVRAALERMAAVVDKQNEGDAAYTPMVGRFDTSPAFLAACD
                     LVFKGTAQPNGYTEPLLHH"
BASE COUNT          375 a          405 c          391 g          413 t
ORIGIN      
        1 cagtggtgta acaaaggctc agtgtaacca tttggttggg ccgtgccttt gaataccaaa
       61 tcacaagctg ccaagaaagc aggagaagta tcgaaacgac ccaccatagg ggtgtaagcc
      121 gcatcacctt cattttgctt gtctaccaca gcagccatgc gttccaatgc tgcacgaact
      181 tcggcttcag tcaccacacc atgcaccaac cagttggcaa tgtgttggct ggaaatgcgc
      241 aaagtggcac ggtcttccat caaaccaatg ttgtggatgt caggcacttt tgagcagccc
      301 acgccatgtt ctacccagcg caccacatag cccaagatgc cttggcaatt gttgtctagt
      361 tcttgttgct tgtcagcagc agaccaattt gtgttttcag ccaaaggtgg cgtcaacaat
      421 tcgttcaaca acgaagcctg tggcgcggcc aagatttgtt tttgcacgtc aaacacattc
      481 acttggtggt aatgcaaggc atgtaaggca gcaccagtcg gtgatggaac ccaagcagtg
      541 gtggcaccag atttaggatg gccaattttt tgttccaaca tttccgccat caagtcaggc
      601 atggcccaca tgcctttgcc gatttgcgct ttaccagcca agccacaggc caagccgatt
      661 tgcacgttgc gggtttcgta agccttgatc caaccggtgt ttttgatgtc gcctttgcgc
      721 aataccgcgc cttggtacat agaggtgtgc atttcatcgc cagtgcggtc taagaagccg
      781 gtgttgatga ataccacacg gtctttggct tgagcgatac aggcttttaa gttggctgaa
      841 gtgcggcgct cttcatccat gatgcccatt ttcaaagtgt tggcaggtag gctcaccaat
      901 tgctcggcag cgccaaacaa acggttggca aatgccactt cttcagggcc atgcattttc
      961 ggtttcacaa tgtaaactga acccgtgcgg ctgttggtgt tttcgccacg gctcaagtcg
     1021 taatgggcaa tcaacgctgt catcacgcaa tccatgatgc cttctggaat ttcgttgccg
     1081 tttttgtcca aaatcgctgg tgtggtcatc aagtggccca cgttgcggat gaacaacaat
     1141 gagcgacctg gcaaggtgat ggcttggcca tctttacctg tgtacacacg gtcggcattc
     1201 atgcgacggg taaaggtttt gccacctttg ctgatttctt ctgccaagct gccttgcatc
     1261 aagccaaacc agttttggta caccaaggtc ttgtcttcac cgtctacagc tgccacagaa
     1321 tcttcacagt ccatgatggt agacaaagct gcctccatga tgacgtcttt gatgccagca
     1381 gggtcttggg caccgatggc gctggttttg tcgataacca catcaaaatg caagccattg
     1441 tgcaagaaca acaatgaagc aggagccgca gtgtcaccag tgtaagccac aaataaggca
     1501 gggttttgta agctgacttt agagccgttt tgtaaagtgg cttccacttc accgttgact
     1561 atcgcataag ccgtggtgtc ttta
//
LOCUS       DAOMVK010000536         1582 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_22769_length_1582_cov_3.025540, whole genome shotgun sequence.
ACCESSION   DAOMVK010000536 DAOMVK010000000
VERSION     DAOMVK010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22769_length_1582_cov_3.025540"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1106
                     /locus_tag="PK856_12495"
     CDS             <1..1106
                     /locus_tag="PK856_12495"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL03232.1"
                     /translation="LSTGLTIPLVSLLLSQRGVANDWLGIAAAMPAIGMLLASLCAHR
                     LSKHLANKTLLLFASGLSALSILAIAFCDSLLWLMVWRFLMGVGCGLLVVLGETWVNV
                     ISAPQWRGRCVAAYATMYTLCQVAGPSVLAWFGSNAVWPLWIAFACNVLGFALLLPSK
                     CEMALGEDTGKHSMTPLAVIRFAPAVILAIFTFAFFDTAMLALLPLYGLQYGLSEQTA
                     VLAVSVLFIGDALFQMPLGWLADRFGAKRVHSYCARFFIMFLLVLPWAMSSMALWLVL
                     LLLGASAGGLYTLGLIRVGGRFEGQDLVAANAAIGIVWGVGSLTGPLISNGLMNGLGS
                     NGLLYGLVGLSVVFLLCSRWQKSLQTVPTGRLG"
     gene            complement(1209..>1582)
                     /locus_tag="PK856_12500"
     CDS             complement(1209..>1582)
                     /locus_tag="PK856_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03233.1"
                     /translation="PSWLERITDLLIRHPDIKIELFQDRLQAYTTVFADTGFALQAAS
                     TGLSVGFSLDPHHWLVCHIPEAFWQQMAQCWCAPAPEAGKQTLLVLAADASQAQLFNA
                     LMYKNAHSYVYAQQEQRARLT"
BASE COUNT          271 a          336 c          503 g          472 t
ORIGIN      
        1 gtttgagtac gggtttaacc atacccttag tcagtttgtt attgagtcag cgcggagtgg
       61 ccaacgattg gttgggtatc gccgctgcca tgcctgccat cggcatgttg ctcgcctctt
      121 tgtgtgccca tcgcttgtcc aaacaccttg ccaacaaaac cttgttgctg tttgcttctg
      181 ggctgagtgc gctcagcatt ttagcgattg ctttttgtga cagcttgttg tggttgatgg
      241 tttggcggtt tttgatgggc gtgggttgcg gcctgttggt ggtgttgggc gaaacttggg
      301 tcaatgtgat ttcggcgccg caatggcgtg ggcgctgtgt ggcggcgtat gccaccatgt
      361 ataccttgtg ccaagtggca gggccgagtg tgttggcatg gtttggctcg aatgcggtgt
      421 ggccgctgtg gattgcgttt gcctgcaatg tgttgggctt tgccttgttg ctgccgagca
      481 agtgtgagat ggctttgggc gaggacacag gcaaacacag catgacgccg ttggcggtga
      541 tccgttttgc ccctgcggtg attttggcaa ttttcacgtt tgcgtttttt gataccgcca
      601 tgctggcctt gttaccgctg tatggcttgc aatacggttt gagtgaacaa accgcggtgt
      661 tggcggtgag tgtgttgttc attggtgacg cgttgtttca aatgcctttg ggttggttgg
      721 cagaccgttt tggtgccaaa cgggtacatt catattgcgc acgctttttc atcatgtttt
      781 tactggtgtt gccgtgggcg atgagcagca tggctttgtg gctggtgttg ttgttgctcg
      841 gagccagtgc aggcggcttg tataccttgg gtttgatacg cgtgggcggc cgatttgagg
      901 gccaagattt ggtcgccgcc aatgcggcca tcggtattgt gtggggtgtg ggcagtttga
      961 ctggccccct catcagcaat ggcttgatga atgggttggg cagcaatggt ttgttgtatg
     1021 gcttggtggg cttgagcgtg gtgtttttgt tgtgttcacg ttggcaaaaa tcgctccaaa
     1081 ccgtgccgac aggccgcttg ggttaaagcc tcgcaaaccc tgttttataa ttcatatgta
     1141 gcaaataagc ggcctgtaaa cccatagaag gtttacaggc cgctttgcat tatggtttta
     1201 acacttattt aagtaaggcg tgcgcgttgt tcttgctggg catagacgta gctgtgggca
     1261 tttttgtaca tcaaggcatt gaacagctgc gcttggcttg cgtcggcggc caataccaac
     1321 agggtttgtt tgcctgcttc aggggctggg gcgcaccaac attgtgccat ttgttgccaa
     1381 aaagcctcgg gtatgtggca caccagccaa tggtgtgggt ctaggctgaa gcccacgctc
     1441 aagcctgttg atgcggcctg taaggcaaaa cctgtgtcgg cgaacacagt ggtgtaagcc
     1501 tgtaggcggt cttgaaacaa ttcaattttg atgtcgggat ggcggatgag caagtcggtg
     1561 atgcgctcta gccacgaagg cg
//
LOCUS       DAOMVK010000537         1580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_22802_length_1580_cov_3.428197, whole genome shotgun sequence.
ACCESSION   DAOMVK010000537 DAOMVK010000000
VERSION     DAOMVK010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22802_length_1580_cov_3.428197"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(277..825)
                     /locus_tag="PK856_12505"
     CDS             complement(277..825)
                     /locus_tag="PK856_12505"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRL03234.1"
                     /translation="MNHLYQPTHPDSLLQADWLALHEHMPPAKWGSLPSFSGAAIWLG
                     MHHSLRKGQNELDYLNRQMLEQQLDWSDYKQRVLQAAWMHHGHLEGHHHYEDHSCFPT
                     LLKLAPQLGKGFDLLEHDHELVAECVSLTRSLLNTVKHSTEPQLDLVERLHTAVQTQG
                     TLLYRHLADEEDLVIPVLALNA"
     assembly_gap    855..864
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(897..>1580)
                     /locus_tag="PK856_12510"
     CDS             complement(897..>1580)
                     /locus_tag="PK856_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRL03235.1"
                     /translation="HKGFETVTIAYQGEVAHRDSTGGGGVIRAGDVQWMTAGAGIVHE
                     EFHSEAFSQSGGLFEMVQLWVNLPAKDKNTPARYQHLSKETIPVVDLADGAGTVRLIA
                     GDFEHTHGAADTFTEMNVWDVDVKAGKEAHISVPAKHSLSMVVLRGEALFNGKDAAKA
                     GQLVGFEDGEGTVSITAVGDDVKILLLSGIPIDEPVVGYGPFVMNTEDEIRTAIDDFN
                     SGRFGRIPA"
BASE COUNT          339 a          420 c          410 g          401 t
ORIGIN      
        1 aatagccttc gtgcacatgt ttggaaaatt ctttgtgttg gtattgggct ttcaacactt
       61 ccaaaccgcc gagctcgggg caatgccaaa attgggtgaa ttcggttttg ccttgcatgg
      121 tgtgtggttt caatcacgcc gtaaaatcct gtttctaccg cacagatgcg caggcgtcta
      181 gaacaaaatt gctcgctagc gctttgaaaa accaaacaag cggcctgtaa ttgtttacag
      241 gccgcttgtt ggtgcttact taattatgca agtggtttaa gcattcaacg ccaatacagg
      301 tatcaccaag tcttcttcat cggccagatg gcggtagagc agcgtgcctt gggtttgcac
      361 ggcggtgtgc aaacgctcca ccagatccaa ttgtggctcg gtgctgtgtt tgacggtgtt
      421 gagcaaggaa cgggttaggc tcacacattc ggccaccaat tcgtggtcgt gctccaacaa
      481 gtcaaagcct ttgcccaatt gcggcgcaag cttgagcagg gttggaaaac agctgtggtc
      541 ttcgtagtgg tggtgtccct ctaagtggcc atggtgcatc catgcggcct gtaacacccg
      601 ttgcttataa tcagaccaat ccaattgttg ttccaacatt tggcgattca ggtaatccaa
      661 ctcgttttgg cctttgcgca agctgtggtg catgcccaac caaatggcgg cgccgctaaa
      721 actgggcaaa ctgccccatt tggcgggcgg catgtgctca tgcaatgcca gccaatcggc
      781 ctgtagcaag ctgtcgggat gggtgggttg gtacaaatgg ttcatgtctt tcctttcagg
      841 ccgctgtcag cctgnnnnnn nnnngagtgg cccaacccgt gagggttgga gcgacatcaa
      901 gcaggaatgc gaccaaaacg tccgctgtta aaatcgtcta tcgcggtgcg aatttcgtct
      961 tcggtgttca tcacaaatgg gccataaccg accactggct catcaatcgg gatacctgag
     1021 agcaacagaa ttttcacgtc atcgcccacg gcagtgatgc ttactgtgcc ttcaccgtct
     1081 tcaaagccta ccaattggcc tgctttggca gcgtctttgc cattgaacaa ggcttcacca
     1141 cgcaatacca ccatggacaa gctgtgcttg gctggcacgc tgatgtgggc ttctttgcct
     1201 gctttcacgt ccacatccca cacattcatt tcagtgaagg tgtcggccgc gccgtgggtg
     1261 tgttcaaaat cacctgcaat caagcgcaca gtgcctgcac cgtcggccaa gtccaccaca
     1321 gggatggtct ctttgctcaa gtgttggtag cgagcagggg tgtttttgtc tttggcaggc
     1381 aggttcaccc acaattgcac catctcaaac aggccaccgc tttggctgaa ggcttcagaa
     1441 tgaaattcct cgtgcacaat gcctgctcca gccgtcatcc attgcacatc gccagcgcgt
     1501 atcacgccgc cgccaccagt ggaatcgcgg tgggccactt cgccttggta ggcgatggtc
     1561 acggtttcaa atcctttgtg
//
LOCUS       DAOMVK010000538         1578 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_22829_length_1578_cov_2.793171, whole genome shotgun sequence.
ACCESSION   DAOMVK010000538 DAOMVK010000000
VERSION     DAOMVK010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22829_length_1578_cov_2.793171"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="PK856_12515"
     CDS             <1..713
                     /locus_tag="PK856_12515"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HRL03236.1"
                     /translation="NAQHVHGNNGLGGMQLHKPKTPLQALHAVAYMVDLLTHAEPYSI
                     TICAVGPLTNIAHMLSLAPEASRGIKEIVIMGGSYFHPGNVTPMAEFNFYVDPHAAQV
                     VLQAGVPLRILPLDVTHQALLTRERIQSLAAIKNENGPRLATLLDHYERHEQASLHEG
                     GAPLHDPCAIACALIPELFEQKRVHVAVETQGQLTMGACVVDWWNKSKAIPNANWATA
                     VDDEAFFDLFNTAIAALP"
     gene            757..1284
                     /gene="msrA"
                     /locus_tag="PK856_12520"
     CDS             757..1284
                     /gene="msrA"
                     /locus_tag="PK856_12520"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HRL03237.1"
                     /translation="MQTALLAGGCFWCLEAVFASIKGVNSVKSGYTAGHTEQPTYAQV
                     CTGQTGHTEAVQIDFDEHTISYAELLTIFFAIHNPTTLNRQGNDVGTQYRSGIYYLDE
                     AQQAAAQAAIAQAQTQHAEPIVTELQAASTFYPAESEHDDYFRLHPYQGYCQLVIAPK
                     VLQAREEFAHHWRQD"
     gene            1340..>1578
                     /locus_tag="PK856_12525"
     CDS             1340..>1578
                     /locus_tag="PK856_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HRL03238.1"
                     /translation="MLTTQFQRIGIVCRQDTPQAFEPLAQIVQALLEDGLQVYLDESN
                     RPELLALPHLAQCEFASKADLGKLCHLVMVLGGDGT"
BASE COUNT          389 a          432 c          381 g          376 t
ORIGIN      
        1 ttaacgctca acatgtacat ggcaacaatg gtttgggtgg catgcagttg cacaagccga
       61 aaacaccgtt gcaagccttg catgcggtgg cttatatggt ggatttgctc acccatgccg
      121 agccttacag catcacgata tgtgcggtag gtccactcac caacattgcc catatgctca
      181 gtttggcgcc tgaagcctcg cgcggcatca aggaaatcgt catcatgggc ggcagctatt
      241 tccacccagg caatgtcacg ccgatggccg aattcaattt ttatgtcgac ccacatgccg
      301 ctcaagtagt gttacaggcc ggtgtgccgc tgcgcatcct gcctttggat gtgacccacc
      361 aagccttgct cacgcgcgag cgcattcaaa gtttggctgc aataaaaaat gaaaatggcc
      421 cacgcttggc cactttgctc gaccattatg agcgccatga acaagcttct ttgcatgaag
      481 gcggtgcacc cttacacgac ccttgtgcca tcgcctgcgc cctcattcct gaattgtttg
      541 aacaaaaacg tgtacacgtg gcagtagaaa cccagggcca gctgaccatg ggggcttgtg
      601 tggtagactg gtggaacaaa tccaaagcca tacccaatgc aaactgggcc acagccgtgg
      661 atgatgaggc tttttttgac ttgttcaata ccgccattgc ggcattgcct taatcggttg
      721 catttgacct gctgaacgtt atcgaaagga attctcatgc aaactgccct cctcgctggc
      781 ggctgttttt ggtgtttgga agcggtattt gcttccatta aaggggtaaa cagtgtgaaa
      841 agcggctata ccgcaggtca cactgagcag cccacttatg cgcaagtgtg cacaggccaa
      901 acaggccaca ccgaagccgt gcaaattgat tttgatgagc acaccatcag ctatgccgaa
      961 ttactgacta ttttctttgc catccacaac ccgaccacct taaaccgcca aggcaatgac
     1021 gtgggcaccc aataccgctc aggcatttac tacctcgacg aagctcagca agctgcggca
     1081 caagccgcca tcgcacaagc gcaaacccag catgccgaac ccatcgtgac agaattacag
     1141 gccgcatcaa cgttctaccc tgccgagtcg gaacacgatg attatttccg tttgcatccg
     1201 tatcaaggct attgccaatt ggtgattgcc cccaaagtct tgcaggctcg tgaagaattt
     1261 gcccaccatt ggcgtcaaga ttgatgtcag tccaacgagt ttgcggtaca gttacgcagg
     1321 tttgccacat gggaacaaca tgctcactac ccaatttcag cgcataggca tcgtctgtcg
     1381 ccaagacacc ccacaagcat ttgagccttt ggcccaaatt gtccaagctt tgcttgaaga
     1441 tggtttacaa gtgtatttgg atgaaagcaa tcggcctgag ctactggcct tgccccactt
     1501 agcacaatgt gaatttgcca gcaaagccga tttgggcaag ttgtgccatt tggtcatggt
     1561 attgggtggc gatggcac
//
LOCUS       DAOMVK010000539         1575 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_22888_length_1575_cov_3.151316, whole genome shotgun sequence.
ACCESSION   DAOMVK010000539 DAOMVK010000000
VERSION     DAOMVK010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22888_length_1575_cov_3.151316"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PK856_12530"
     CDS             complement(<1..414)
                     /locus_tag="PK856_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRL03239.1"
                     /translation="MRNMAISIIAMMVLAACNQGGGAAAGGDSKAASGSSAQTSSSGG
                     GDGKEILIGFSAPLTGPQSHYGEDYKSGVQMAIDEANEQGITINGAPAKFKLDAQDDA
                     ADPKQATQVAERFVDQKVAGVIGHFNSGTTIPASKV"
     gene            complement(548..>1575)
                     /locus_tag="PK856_12535"
     CDS             complement(548..>1575)
                     /locus_tag="PK856_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRL03240.1"
                     /translation="IGVVGVVLPWNFPAQMFAWKVAPALITGNSVVVKPAELTSLSAY
                     RLVELAYEAGVPKGALSLVCGLGEKVGEALGRHMDVDMVSFTGSTEVGRYFLKYSAES
                     NLKEIVLECGGKSPQVVFADADLDAAVPHILNAAFWNMSENCSCGSRLIVDASVKDAL
                     LEKVKAGVSSWKLGDPRDEDVVVGPMVEGPHFEKVKSFIATTEKEGGKLVQGGKIHSD
                     LGSGWYIEPTIFDNVTADMTLFQQEVFGPILAVTTFTSEEEAVALANDSTYGLAASLY
                     TGNLRCAQRVSRAIRAGTVSINGFSEGDITTPFGGFKQSGFGGRDKGLEALQQYVQTK
                     TIWYVQD"
BASE COUNT          396 a          447 c          328 g          404 t
ORIGIN      
        1 aactttagac gctggaatgg tggtaccaga gttgaagtga ccgatcacac ccgctacttt
       61 ttggtctacg aaacgttcag ccacttgggt ggcttgtttt gggtcggctg catcgtcttg
      121 tgcatccaat ttgaatttgg caggcgcacc gttaatggta atgccttgct cattggcttc
      181 atcaatggcc atttgcacac cgctcttata gtcttcacca taatgcgatt gtgggccagt
      241 taatggcgct gagaaaccaa tcaagatttc tttgccatcg ccacccccac tgctgctggt
      301 ttgtgcgcta ctgccagatg ctgctttgct gtctccaccc gccgctgcgc cgccgccttg
      361 gttacaggcc gctaaaacca tcatagcaat aatgctgatt gccatatttc tcatatttct
      421 tcctttatta tttggtgata atcacttgtg ttacaacttg ggtaatacat ttttgaaaac
      481 caacaaagcc agccgcctga tttttgttat gggcaactgg ctggtacgac attacaacac
      541 tattacatca atcttgtaca taccaaattg tcttggtttg cacatactgt tgtaaagctt
      601 ctaaaccttt gtcacgaccg ccaaaacccg attgcttaaa tccaccaaat ggggtagtga
      661 tgtcgccttc agagaagcca ttaatcgaca cagtgcccgc acgaatcgca cggctcacac
      721 gttgggcaca acgcaggttt ccagtgtaca aagaggcagc caagccgtaa gtgctgtcat
      781 tggccaaagc cacggcttct tcttcgctgg tgaacgtggt caccgccaag atggggccaa
      841 acacctcttg ttggaacaaa gtcatgtcag cagtcacatt gtcgaaaatg gttggctcga
      901 tataccagcc actgcccaaa tcactgtgta tcttgccgcc ttgcaccaac ttgccgcctt
      961 ctttttccgt ggtcgcgata aagcttttca ctttttcaaa gtgtgggcct tcaaccatgg
     1021 ggcccaccac cacatcttca tcgcgtggat cgcccaattt ccaagaagac acgccggctt
     1081 tcactttttc aagcaaagcg tctttcacgc tggcatccac aatcaaacgc gagccacaag
     1141 aacagttttc agacatgttc cagaacgctg cattcaaaat gtgaggcaca gccgcatcca
     1201 aatcggcatc ggcaaacacc acttgcgggc tcttaccacc acactccaac acgatttctt
     1261 tcaaattgct ctcggcagag tatttcaaga aatagcggcc cacttcggta gaacctgtga
     1321 acgacaccat gtccacgtcc atgtgacggc ccaaggcttc gcccactttt tcgcccaaac
     1381 cgcacaccaa gctcaacgca cctttaggca cgcctgcttc gtaagccagt tcaaccaaac
     1441 ggtaagcaga caaagaagtc aattcagcag gttttaccac cactgagttg cctgtaatca
     1501 atgcaggagc cactttccaa gcaaacattt gtgcagggaa attccatggc aacaccacac
     1561 ccaccacgcc tatgg
//
LOCUS       DAOMVK010000540         1573 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_22918_length_1573_cov_1.938076, whole genome shotgun sequence.
ACCESSION   DAOMVK010000540 DAOMVK010000000
VERSION     DAOMVK010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22918_length_1573_cov_1.938076"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="PK856_12540"
     CDS             complement(<1..968)
                     /locus_tag="PK856_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRL03241.1"
                     /translation="MNLVFGILLCLPIIASLLPLLRCSHWTVRIFDFPRLQIAFVSLL
                     LIIASVIITNEPMWMLALQIINALCLLYQLYKISAYGPWHRKQVLDAQGPDDERTISI
                     LNSNVLTPNREYQKVLDMVERYQPDLFLTLETDAWWEDKLKPLEQHYPYTVKIPLDNL
                     YGMHLYSRLELSDTEVRHWVKEDIPSIVSKVILRSGEHIKIYCLHPEPPAPGESDVST
                     PRDAELLLAGKEIDHTDETVIVFGDMNDVAWSHTTRLFQKISGLLDPRVGRGLFNTFH
                     AHHWFLRWPLDHMFVSNDFLVKKLLRLGNVGSDHFPVYAKFQYHPKA"
     gene            complement(1312..>1573)
                     /locus_tag="PK856_12545"
     CDS             complement(1312..>1573)
                     /locus_tag="PK856_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HRL03242.1"
                     /translation="FGEAVLLDDDINIDFAAVTTPNDRVAVIATLPLTTNEQWQQLAI
                     NELVCLRAGEVVFSDAPEPKQYMSIEEGLCIARSVGAAGSID"
BASE COUNT          406 a          352 c          408 g          407 t
ORIGIN      
        1 gctttcggat gatactgaaa tttagcataa acggggaagt ggtcggaacc cacattgccc
       61 aaacggagca gttttttcac cagaaagtca ttgcttacaa acatgtggtc tagtggccag
      121 cgcaaaaacc aatggtgggc atggaaggta ttgaacaggc ctcggcccac gcgcgggtcg
      181 agcaagccac tgattttctg aaacaatcgg gtggtgtgtg accaggccac atcgttcatg
      241 tcgccaaaca caatcacggt ttcatcggtg tggtcgattt ctttgcccgc gagcaacaat
      301 tccgcatcgc gtggcgttga aacatcgctt tcccctgggg ctggtggctc tgggtgcaga
      361 caataaatct tgatgtgctc gcctgaacgc aaaatcacct tggaaacaat tgatggaatg
      421 tcttctttta cccagtggcg cacttcggta tcgctcaatt ctaagcgact gtataaatgc
      481 atgccataca aattgtctaa ggggattttg acggtataag ggtagtgttg ctccagtggc
      541 ttgagtttgt cttcccacca tgcatcggtt tctagggtta aaaacaaatc aggctggtag
      601 cgctctacca tgtcgaggac tttttgatac tcgcggttgg gggtgagcac gttgctgttg
      661 aggatggaaa tggtgcgctc atcatctggg ccttgcgcat ccaagacttg cttgcggtgc
      721 caagggccgt aagcgctgat tttatacaat tgatacagca aacacagtgc attgatgatt
      781 tgtaaggcca gcatccacat cggttcattg gtgatgatga cgctggcgat gatcaacaac
      841 aggctgacaa aggcaatttg caaacgcgga aaatcaaaaa tgcgcaccgt ccaatggcta
      901 cagcgcagca aaggcagcaa gctggcaatg atgggaaggc acaataagat gccaaaaacc
      961 aaattcatgc atggcctttc gtgggctgac ggtgtgtgcg ccattcactc acagctaaag
     1021 tgtacaaaga ttgggaagac atgtggataa tcataacaag tgtttggtct gtatggtgtt
     1081 tttaattctg cttaaatatt aagcattcaa gcaataagcg gcctgtaaac gtcatcgtgt
     1141 tggtttatac acatctcatc cacagtttta tccctattgc atgtgacaga ctggttctgt
     1201 ggtgaattaa ttccccgcta tagacaacag acgggggcaa gcaacgggat atcatgtgga
     1261 taagctgttt aaatgtttga tttgatggtg ttaataaaaa ctgcttaatt tttaatcaat
     1321 agagcctgcc gcacccacac tgcgagcgat gcacaagcct tcttctatgc tcatgtattg
     1381 cttgggttcg ggcgcatcgc tgaacaccac ttcacctgcg cgcaaacaca ccaattcatt
     1441 gatggccaat tgctgccatt gctcattggt ggtgaggggg agggtggcaa tcacggccac
     1501 gcggtcgttg ggcgtggtga cggcggcgaa atcgatgttg atgtcgtcgt cgagcaatac
     1561 cgcttcacca aac
//
LOCUS       DAOMVK010000541         1569 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_22987_length_1569_cov_4.056803, whole genome shotgun sequence.
ACCESSION   DAOMVK010000541 DAOMVK010000000
VERSION     DAOMVK010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22987_length_1569_cov_4.056803"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="PK856_12550"
     CDS             complement(<1..664)
                     /locus_tag="PK856_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRL03243.1"
                     /translation="MKNAYIIDAIRTPFGRYGGELASVRADDLGALPIKALMERNSSV
                     NWEAVDDVIYGCANQGGEDNRNVGRMSALLAGLPYQVPATTVNRLCGSSLDAIAMAAR
                     AIKAGEADLIIAGGVESMTRAPYVMGKSDGAFGRGQKIEDTTMGWRFINPKLKELYGV
                     ETMPQTAENVAEQFHVSRADQDQFALTSQQRTAAAQARGYFDKEIIAVTIPQKKGDPV
                     VVD"
     gene            complement(728..1378)
                     /locus_tag="PK856_12555"
     CDS             complement(728..1378)
                     /locus_tag="PK856_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004925445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HRL03244.1"
                     /translation="MTYTKLTRDQIAARVAQDIPDGAYVNLGIGLPTKISKYLPSDKD
                     VFLHSENGLLAFGPPPPAGEEDPELINAGKEYVTMLTGGSFFHHGDSFAMMRGGHLDI
                     CVLGAFQVASNGDLANWHTGAPDAIPAVGGAMDLAVGAKKVFVTTDHVTKSGEPKIVA
                     ELTYPVTGKKCVDRIYTDLCVIDVTPTGLKVIEKVDGLGFDELQALTGASLIDATL"
     gene            complement(1391..1561)
                     /locus_tag="PK856_12560"
     CDS             complement(1391..1561)
                     /locus_tag="PK856_12560"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HRL03245.1"
                     /translation="MVYRKSARNFGPIMATASDCTIAQVTEVVALGELDPEHIITPGI
                     FVQHVVQVAPAQ"
BASE COUNT          341 a          441 c          406 g          381 t
ORIGIN      
        1 tgtccaccac cacagggtcg ccttttttct gcggaatggt cacggcgatg atttctttgt
       61 cgaaataacc gcgcgcttgc gccgccgcag tgcgctgttg gctggtcaac gcaaattggt
      121 cttggtcggc acgcgacaca tggaattgct cggccacgtt ttcagcggtt tgcggcatgg
      181 tttccacgcc gtacaattct tttaatttcg gattgatgaa acgccagccc atggtggtgt
      241 cttcaatttt ttgaccgcgg ccaaatgcgc catcggattt gcccatcaca tacggtgcac
      301 gcgtcatgga ttccacgcca ccggcaatga tgaggtcggc ttcgcctgct ttgatggcac
      361 gcgccgccat cgcaatcgca tccaaagacg agccgcacaa acggttgacc gtggtggcgg
      421 gcacttggta aggcaagcct gcgagcaagg ccgacatgcg accgacattg cggttgtctt
      481 cgccgccttg gttggcacag ccataaatca catcatctac cgcttcccaa ttcacactgc
      541 tgttgcgttc catcaaggcc ttaattggca aagcccccaa atcatcggca cgcacactcg
      601 ccaattcgcc gccatagcgg ccaaacgggg tgcggatggc atcaattatg taagcgtttt
      661 tcatctgttc ttccttggtc atttgttctt ttgggtgaac agcctgtgtt tacaggccgc
      721 ttatggctta aagggtggca tcgatcaagc ttgcaccggt caaagcctgc aattcgtcaa
      781 agcccaaacc gtcaactttt tcaatgactt tcaagcctgt aggcgtgacg tctatcacgc
      841 acaaatcggt gtaaatgcgg tcgacacatt ttttccctgt cactgggtaa gtcaattcgg
      901 caacgatttt cggctcgccc gatttggtca catggtcggt ggtgacaaac acttttttcg
      961 cccccacagc caagtccata gcgcctccca cagcagggat ggcatcgggt gcgcctgtgt
     1021 gccagttggc caaatcacca ttggacgcca cttgaaacgc gcccaacaca caaatgtcca
     1081 aatggccgcc acgcatcatc gcgaacgagt cgccatggtg gaaaaacgag ccgcctgtga
     1141 gcatggtgac gtattctttg cctgcattaa tcaactcagg gtcttcctca cctgctggtg
     1201 gcggtgggcc aaatgccaac aagccgtttt cagaatgcaa aaacacgtct ttatctgaag
     1261 gcaaatactt ggaaatcttg gtgggcaagc ctatgcccaa attcacatac gcgccgtcgg
     1321 gaatgtcttg cgccacacgg gcggcaattt ggtcacgcgt cagtttggta taagtcatgg
     1381 ttgtgtctcc ttactgcgcg ggcgcaactt gcaccacgtg ttgaacaaaa atgcctgggg
     1441 tgatgatgtg ctctgggtcg agctcaccca aagccaccac ttctgtcact tgggcgatgg
     1501 tgcaatcaga ggcagtggcc atgatggggc cgaagttgcg cgccgatttg cggtaaacca
     1561 aattgcccc
//
LOCUS       DAOMVK010000542         1566 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_23057_length_1566_cov_3.785572, whole genome shotgun sequence.
ACCESSION   DAOMVK010000542 DAOMVK010000000
VERSION     DAOMVK010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23057_length_1566_cov_3.785572"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="PK856_12565"
     CDS             <1..940
                     /locus_tag="PK856_12565"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009119912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HRL03246.1"
                     /translation="WSSSSSQLLTGHSPLHQQLEATLAHLFQREAALTFASGYQMNFG
                     LLNALSTGNVLILADKLVHASMVDGLLISKAKFLRYHHNDMAHLHALLCQHHAAFERI
                     IIVTESVFSMDGDIADLQQLVAFKREFANVALYVDEAHAIGVRGESGLGCAEEQGCVA
                     EIDFLVGTFGKAVHSVGGYVLCSRLARDYLINHMRPLIFSTALPPINMAWTDFVLQRL
                     HSLQAERTHLQQLSSALINKVNTLQGHCVSQSHIVPVLCGSNAAALAQAQALREHGML
                     VMAVRPPTVPKNQARLRISLNAGLSHEQLDALMACL"
     gene            931..>1566
                     /locus_tag="PK856_12570"
     CDS             931..>1566
                     /locus_tag="PK856_12570"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRL03247.1"
                     /translation="MLMIQKDLVQHRFEKAFASYGEHASLQQTMSDTLFAQWQSHAPQ
                     VASLLELGCGTGNLSRLLTHLPDLQTLYLNDLCAASACMSHEFTGRLRSEFISGDMDN
                     IAFPSHLDAVVSSSALQWSQDLPALLTKISASLKPQGWLAFASFGPSHYQEIKALTGI
                     GLHYPSLADIAQYLQADFDLLWQSETPHTQGFANPMAVLQHMRQTGVSGISK"
BASE COUNT          351 a          452 c          409 g          354 t
ORIGIN      
        1 ttggtcgtcc agctcatcgc aattgctcac gggccactcg ccgctgcacc aacaactcga
       61 agccaccttg gcgcacctgt ttcagcgcga agccgctttg actttcgcca gtggctacca
      121 aatgaatttt gggctgttaa acgctttgtc gacgggtaat gtgttgatat tggccgacaa
      181 actggtgcat gccagcatgg tcgatggctt gctcatcagc aaggcaaaat tcctgcgcta
      241 tcaccacaat gacatggcgc acctgcacgc cttgctgtgc caacaccatg ccgcgtttga
      301 acgcatcatc atcgtcaccg aatcggtgtt cagcatggat ggcgacatcg ccgatttgca
      361 gcaattggtc gcttttaagc gcgaatttgc caacgtggct ttgtatgtgg atgaagcgca
      421 cgccatcggt gtgcgcggcg agagtggctt gggctgcgcc gaagaacaag gctgcgtggc
      481 ggaaatagat ttcttggtgg gcacgtttgg caaagccgtg cattcggtgg gcggctatgt
      541 gttgtgttca cggctggcac gcgactacct catcaaccac atgcgacctt taattttcag
      601 caccgccttg ccgccgatta acatggcatg gacagatttt gtgttgcaac gcctgcacag
      661 cttacaggcc gagcgcacgc atttgcagca attgagcagc gctttaatca ataaagtgaa
      721 cacattgcag gggcattgtg tgtcgcaatc gcacatcgta cctgtgctgt gtggcagcaa
      781 tgccgccgcc ctcgcccaag cgcaagcctt gcgcgaacac ggcatgttgg tgatggcggt
      841 gcgcccaccc accgtaccga aaaaccaagc gcgtttgcgc atctcgctca acgcaggctt
      901 gagccatgag cagcttgatg cattgatggc atgcttatga tccaaaaaga cttggtacaa
      961 caccgctttg aaaaagcctt tgcctcgtat ggcgaacacg ccagtttgca gcaaaccatg
     1021 agcgacactt tgtttgcgca gtggcaaagc catgccccgc aagtggcgag cctattggaa
     1081 ttgggctgcg gcaccggcaa tcttagccgt ttactcaccc acttgcccga cttgcagacc
     1141 ctgtatttga acgatttgtg tgcggcctca gcttgcatgt cacacgaatt tacaggccgc
     1201 ttgcgcagtg aatttatcag cggtgacatg gacaacatcg cgtttcccag ccatttagat
     1261 gccgtggtgt ccagctcagc cttgcaatgg tcgcaagact tgcctgccct actaaccaaa
     1321 atcagcgcca gcctcaagcc gcaaggctgg ctggcgtttg ccagcttcgg ccccagccac
     1381 taccaagaaa tcaaagcctt aaccggcata ggcttgcact accccagcct tgctgacatc
     1441 gcccagtatt tacaggccga ctttgacctg ttgtggcaaa gcgaaacgcc gcacacacaa
     1501 ggctttgcca accccatggc cgtgttgcaa cacatgcgcc aaacaggggt gtctggcatc
     1561 agcaaa
//
LOCUS       DAOMVK010000543         1565 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_23084_length_1565_cov_2.343709, whole genome shotgun sequence.
ACCESSION   DAOMVK010000543 DAOMVK010000000
VERSION     DAOMVK010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23084_length_1565_cov_2.343709"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="PK856_12575"
     CDS             <1..746
                     /locus_tag="PK856_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003789257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL03248.1"
                     /translation="ITRQIPYLLANNNKTVQMQWLPLTRDNVLSKKESQEKAIVLEGQ
                     VFGIAAADLEKTTVDVMLGQEKFVAKVDKNGLFSTPVSGTQLAQAQVKTLQAQVHGPD
                     GVVSAQLNYEVGEAAQVSKLDNHIDAMPLIQADAPMQTVSGKVVKQYSQSWLYFAIIM
                     DNLAAGLAGAAFIAFLSSLTSVSFTAVQYAIFSSLMTLTPKIIGGYSGSIVNSIGYPN
                     FFLFTTLLGLPILFLVWWVARLLKQQTQT"
     gene            816..1223
                     /locus_tag="PK856_12580"
     CDS             816..1223
                     /locus_tag="PK856_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03249.1"
                     /translation="MATILLDFDGTCVAHEYPYVGDDIGAQQVLHDLVSNGHKLILFT
                     MRSDINYLDDALAWFQEQGIPLYGVQSHPEQTSWTASPKAYGELIIDDTMLGAKLVGG
                     RYLNWQWAAEMLCERGLITPAQLAAYDFSLYVY"
     gene            complement(1297..>1565)
                     /locus_tag="PK856_12585"
     CDS             complement(1297..>1565)
                     /locus_tag="PK856_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03250.1"
                     /translation="ASQAASCTRMGDTTYCDDEYGNTRVTGGYGDTVYVDEDGPSGNR
                     SVSASRYGDTVQVDETYGSNTYSTTYGSYGDTNYRDDGDDVPLW"
BASE COUNT          389 a          336 c          421 g          419 t
ORIGIN      
        1 caatcacaag acaaataccg tatttgttgg ccaataacaa taagaccgtg caaatgcaat
       61 ggctgccttt gacccgcgat aacgtgcttt ccaaaaagga atcacaagaa aaagccattg
      121 tgcttgaagg gcaagtgttt ggcattgctg ctgcagattt ggaaaaaacc actgtggacg
      181 tgatgttagg ccaagagaaa tttgtggcca aagtggataa aaatggactg ttttcaaccc
      241 ctgtttcggg tacgcaattg gctcaagctc aagtgaaaac attgcaagcg caagtgcatg
      301 gcccagatgg tgttgtcagc gctcagttga attacgaagt tggggaagcc gctcaagtca
      361 gtaaattgga caaccacatt gatgccatgc ctttgattca agcggatgcc ccaatgcaaa
      421 cagtgtcggg aaaagtggtt aagcaataca gtcagtcatg gttgtatttc gccatcatca
      481 tggataattt ggccgcaggc ttggctggtg ctgcattcat tgccttcttg tccagcttaa
      541 ccagtgtgtc ttttactgcg gtgcaatatg cgattttcag ctctttgatg actttaaccc
      601 ctaaaatcat aggtgggtat tcaggcagca tcgtgaacag catcggttat ccaaactttt
      661 tcttgttcac gaccttgtta ggcttaccaa tattgttttt agtgtggtgg gtcgcgcgtt
      721 tgttaaagca acagacacag acttaggcag aggcttgtgt gactgggata atcaaatcca
      781 tctggcaaca gtgtttcacc aaacgaaagg gcaatatggc gacgatatta ttggattttg
      841 atggcacgtg cgtggcgcat gaatacccgt atgtgggcga tgacattgga gcgcagcaag
      901 tcttgcatga tttggtgagc aatggacata agctgatttt gtttaccatg cgctcagaca
      961 tcaattattt ggacgatgcg ctggcgtggt ttcaagagca aggcattcct ttgtatggcg
     1021 tgcaatcgca tcctgagcaa acatcgtgga cggcttcacc caaggcttat ggtgaactca
     1081 tcatcgacga caccatgtta ggggcaaaat tggtgggtgg gcgttatttg aattggcagt
     1141 gggcggcaga aatgctgtgt gagcgaggct tgattacgcc agcgcagttg gcagcttatg
     1201 atttcagttt gtatgtgtat tgagatgatt cgaaccgatg tcgggataca ttacaggccg
     1261 cttgatgcgg cctgtaagtg ttttcacaaa gtgaacttac cacaatggca catcatcacc
     1321 atcgtcacgg tagttggtat cgccgtagct gccatacgtg gtggaatagg tgttggagcc
     1381 ataagtctcg tccacttgaa ccgtgtcacc atagcggctg gcgctgactg agcggttgcc
     1441 cgatgggcca tcttcgtcga catacaccgt gtcgccatag ccacccgtga cgcgggtgtt
     1501 gccatattcg tcatcacagt aagtggtgtc gcccatgcgc gtgcatgagg cggcttggct
     1561 ggcgg
//
LOCUS       DAOMVK010000544         1559 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_23212_length_1559_cov_1.795213, whole genome shotgun sequence.
ACCESSION   DAOMVK010000544 DAOMVK010000000
VERSION     DAOMVK010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23212_length_1559_cov_1.795213"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..864)
                     /locus_tag="PK856_12590"
     CDS             complement(97..864)
                     /locus_tag="PK856_12590"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HRL03251.1"
                     /translation="MAAENLRVSIAPDIHPEFLAGWHIFLRHLQKQVEVSMDISSYAN
                     FGELRAGLEQGQVDMIFASAADCSYLVLQKNFVALARPESDVIEIVVLCRADAAYQHI
                     EDIEGKQNRIAYADSPEVKHLGLILLEAANVGDGDLVLVSSQSHLLAAKQLMNAEVDL
                     AFVPTDIYEGWSATLRNSMRPLVQTRKEDVEGLSHVLLLSSHYQHLVQPLKSALHHIN
                     GSGQQGLLHDINIHKWHVLDDNTDIDYLIDLVDTLQI"
     gene            complement(864..1370)
                     /locus_tag="PK856_12595"
     CDS             complement(864..1370)
                     /locus_tag="PK856_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009427603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRL03252.1"
                     /translation="MSYIWKDMSEADLAKTHEPKLHLHHFFDGQQRRLFTVDDEVAYP
                     DGRMIVSRTDLNGIITHANQAFIDMSGYEREELIGAPQCILRHPDIPSEVFGDLWETI
                     QSGKSWFGYVKNLRKDGRYYWVYASVNPNFRNGRVVAYTSIRRKPARSKINDTIAHIA
                     ALKCCGEA"
BASE COUNT          425 a          409 c          326 g          399 t
ORIGIN      
        1 cgaagccata caacaggctt ggatagacga gcaagaataa acaaaacgtg cctttgggca
       61 cgttttgtta tgactgactt accttgccaa tgcccatcaa atctgcaagg tatcaaccaa
      121 atcaatcaaa taatcaatgt cggtattgtc gtctaacaca tgccatttgt ggatgttgat
      181 gtcgtgtaac aaaccttgct gaccgctgcc attgatgtgg tgcaacgccg atttcagggg
      241 ctgcaccaaa tgctgatagt gcgaagacaa aagcaacaca tgactcaagc cttccacatc
      301 ttcctttcgg gtttgcacca aaggccgcat gctgttgcgc aaagtcgcac tccagccctc
      361 gtaaatgtcg gtgggcacaa acgccaaatc tacctcagca ttcatcaatt gctttgcagc
      421 aagcaaatgg ctttggctgc tgaccaacac caagtcgccg tcgcccacat tggcagcttc
      481 cagcaaaatc aaacccaaat gcttcacctc agggctgtcg gcataggcaa tgcggttttg
      541 tttgccctct atgtcttcaa tgtgttggta agctgcatcc gctcggcaca acaccacaat
      601 ctcaatgaca tcgctctcgg gccttgccag tgccacaaag tttttttgca acaccaaata
      661 gctgcaatcg gccgcgctgg caaaaatcat gtccacttgg ccttgctcca aacccgctcg
      721 caattctcca aaattggcat aagaagaaat gtccatgctc acttccactt gcttttgcaa
      781 atggcgtaag aaaatgtgcc aacctgccaa aaattcagga tgaatgtctg gcgcgatgct
      841 cacacgcaga ttttccgccg ccatcacgct tctccacagc atttcaaagc ggcaatgtgc
      901 gcgatggtgt cattgatttt agaacgggca ggcttgcggc ggattgaggt gtaagccacc
      961 acgcgaccat tgcgaaaatt ggggtttacc gaggcataca cccagtaata ccgaccatct
     1021 ttgcgcaggt ttttcacata gccaaaccat gatttgccac tttgtatggt ttcccacaaa
     1081 tcaccaaaca cctcactggg gatgtcggga tggcgcaaaa tacactgcgg cgccccaatc
     1141 aattcctcgc gctcatagcc cgacatgtca ataaaggctt gattggcgtg ggtaatgatg
     1201 ccgtttaaat cggtgcgcga cacaatcatg cgcccatcgg gataagccac ttcatcgtct
     1261 acggtgaaca aacgacgttg ttggccatca aaaaaatgat gcaaatgcag tttgggttca
     1321 tgcgtcttgg ccaaatcggc ttcagacatg tctttccaaa tatatgacat gattcatctc
     1381 ctcggtatgg attatttttg gctttatagc tcttatccat tacatttcta tgtcattgat
     1441 tagagtaata ttttagtaaa taccacccat cttctctgtg tgacagcaag cgcacatcac
     1501 gccgataaga ataacaaata actaataaac tttattaacc tagcgcaaac ttttggcac
//
LOCUS       DAOMVK010000545         1557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_23243_length_1557_cov_3.729028, whole genome shotgun sequence.
ACCESSION   DAOMVK010000545 DAOMVK010000000
VERSION     DAOMVK010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23243_length_1557_cov_3.729028"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="PK856_12600"
     CDS             complement(<1..589)
                     /locus_tag="PK856_12600"
                     /inference="COORDINATES: protein motif:HMM:NF016088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HRL03253.1"
                     /translation="MNSIALISLIGTVLGYCLIKKLHQHHRHLYASPAILLPAIIMLI
                     MVLTHVSYAQYMQDTQWIVWLLGPVTVAFAIPIYEYRVLVCEHIIAIGMGIVVGMSAG
                     ILSAWYLSQWFHFSPEITYSLMSRSISTPFAVELASHIHGSAELTTLFTILTGMVGML
                     AGDVVLAVLRLHSHIAEGASLGNAAHGFGSSKAMQR"
     gene            complement(593..1027)
                     /locus_tag="PK856_12605"
     CDS             complement(593..1027)
                     /locus_tag="PK856_12605"
                     /inference="COORDINATES: protein motif:HMM:NF015729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HRL03254.1"
                     /translation="MNTEFSAHTGHTLLKTTAQLALLLLFWAIGYGLQQRFHVPISAG
                     VLGLFLALAALLCGVLKLDWIKGGADFVLGELVLFFVPCVVGLMKYQALFKSQGWQLI
                     STIAIGTVCVMVATAYAVYLGFKLEHALKQRLHAHKPAIHHE"
     gene            1125..1520
                     /locus_tag="PK856_12610"
     CDS             1125..1520
                     /locus_tag="PK856_12610"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRL03255.1"
                     /translation="MVDIKTLTMLVEVVRRQSFSAAAEHLCLTQPTISKAMNGLEQDL
                     GVSLFQKGEAGRKREVTLTYMGELIYQHAQNILAEQQQLYATVAQVGSLKKGKLTLGL
                     PPLGSVLLSALIARFHAAYPDIQLNPLRI"
BASE COUNT          414 a          375 c          427 g          341 t
ORIGIN      
        1 ggcgttgcat cgctttactg gagccaaagc cgtgggcggc attgcccagc gatgcacctt
       61 cggcaatgtg agaatgcaag cgcaacaccg ccaaaaccac atcgcctgcg agcatgccca
      121 ccatgcctgt gaggatggtg aacaaggtgg tgagctcggc cgagccgtgg atgtggctgg
      181 cgagttcgac ggcaaacggg gtggagatgg agcgcgacat caggctgtag gtgatttcag
      241 ggctgaagtg aaaccattgc gacaaatacc atgcgctgag tatgcccgca ctcatgccca
      301 ccactatgcc catgccgatg gcgatgatgt gttcgcacac caagacgcgg tattcataaa
      361 tcggaatggc aaacgccaca gtgacagggc caagcagcca cactatccat tgtgtgtctt
      421 gcatgtattg cgcatagctc acatgggtta agaccatgat gagcatgatg atggcaggca
      481 gcaaaatcgc aggcgaggca tacaaatggc ggtggtgttg gtgcagtttt ttaatcaggc
      541 aatagcccaa taccgtaccg ataagactga ttaaggcgat gctgttcatg gtttactcgt
      601 ggtgtatggc gggtttgtgg gcgtgtaagc gttgttttaa ggcatgttct aatttgaagc
      661 cgaggtagac agcgtaagcc gtggccacca tcacacacac cgtgccaatg gcgatggtgc
      721 tgatgagttg ccagccttgg cttttaaaca gggcttggta tttcatcaaa cccaccacac
      781 agggcacgaa aaacaacacc aattcgccca acacaaaatc ggccccgcct ttaatccaat
      841 ccagtttcaa cacgccgcac agcaaggcgg ccaaggccaa aaacaagccg agtacgcctg
      901 ctgaaatcgg cacatggaag cgctgttgca gcccatagcc tatggcccaa aaaagcagca
      961 ataaagccaa ttgtgcggtg gttttaagca gtgtatggcc tgtgtgggcg gaaaattcag
     1021 tgttcatggt gggtgtcttg tctggcaatg gaaacattgt aaggcgggta aatacggcat
     1081 attatgaata ttcttattaa aattatgaat aattggcatt gtttatggtc gacatcaaaa
     1141 ccctcaccat gttggtggaa gtggtgcgca ggcaaagctt cagtgcggcg gcggagcatc
     1201 tgtgcttgac ccaacccacc atcagcaaag ccatgaatgg attggagcaa gacttgggcg
     1261 tgagtctgtt tcaaaaaggc gaagcagggc gcaaacgcga agtcaccctt acctacatgg
     1321 gcgaactcat ttaccaacat gcgcagaaca ttttggccga gcagcaacag ctgtatgcca
     1381 cggtggcgca agtgggcagc ttgaaaaaag gcaagctgac cttgggcttg ccgcctttgg
     1441 gctcggtatt gctcagtgcg ctgattgcgc gctttcacgc cgcttatccc gacatacagc
     1501 tgaacccgtt gcgcatttag aataaaaaaa agtaagttaa tcacatatca taatgat
//
LOCUS       DAOMVK010000546         1556 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_23257_length_1556_cov_3.038641, whole genome shotgun sequence.
ACCESSION   DAOMVK010000546 DAOMVK010000000
VERSION     DAOMVK010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23257_length_1556_cov_3.038641"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /gene="dapE"
                     /locus_tag="PK856_12615"
     CDS             complement(<1..863)
                     /gene="dapE"
                     /locus_tag="PK856_12615"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HRL03256.1"
                     /translation="MTTNTTLELTKQLMARASVTPLDEGCQQILIDRLMALGFVAERL
                     RFGDTDNIWLRRGTAAPLTCFAGHTDVVPTGPLEQWQSDPFVPVEREGKLYGRGAADM
                     KTSIAAFVTACERLLTEQPDYPASIALLITSDEEGDAHDGTTKVVEILKQRCELIDYC
                     IVGEPTSTNQLGDTIKNGRRGSLSGNLTIIGKQGHIAYPHLAINPIHTFAPALVDLTQ
                     EVWDHGNEYFPATSFQVSNIQAGTGATNVIAGSLNVKFNFRFSTEVTADELKQRVHAI
                     LDQHGLKYDLL"
     gene            1125..>1556
                     /locus_tag="PK856_12620"
     CDS             1125..>1556
                     /locus_tag="PK856_12620"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL03257.1"
                     /translation="MQSLFQNTHSRFFLILLTMGSFSLGMTEYSMIGLSNEIAADLKL
                     TNHQTSQVMSAYAMGVLIGAPLLAILGSKLNRHTLMAYLLLWCACGNLFISFAHSYEQ
                     LRWLRTLNGFPHGLYFSTAALLIYDVFPKNKRANYIGVMFSG"
BASE COUNT          386 a          391 c          355 g          424 t
ORIGIN      
        1 cacagcaaat cgtatttcaa accatgctgg tctaaaatcg catgcacgcg ctgttttaac
       61 tcgtctgcgg tgacttcggt gctgaaacgg aaattgaatt tcacgttcaa gctgcccgca
      121 atcacattgg tcgcgcctgt gcctgcttgg atattggata cttgaaaact ggtggcagga
      181 aaatactcat tgccgtggtc ccacacttct tgggttaaat ccaccaaggc aggcgcaaac
      241 gtgtgtatcg gattgatggc caaatgtggg taggcgatgt ggccttgctt gccgatgatg
      301 gtaaggttgc ccgaaagcga accgcggcga ccgtttttga tggtgtcgcc caattggttg
      361 gtgctggttg gttcacccac gatgcagtaa tcaatcaact cgcagcgctg ttttaaaatc
      421 tccaccactt tagtggtacc atcatgggca tcgccttctt catccgaagt aatcaataaa
      481 gcaatgctgg caggataatc aggctgttcg gtgagcaagc gctcacaagc ggtcacaaaa
      541 gcggcaatcg aggttttcat gtcggctgcg ccgcgaccat acaatttgcc ctcgcgctcc
      601 actggcacaa atgggtccga ttgccactgc tccaacggcc cagtgggcac cacatcggtg
      661 tgccctgcaa aacaggtgag cggcgcagcc gtgcctcggc gcaaccaaat attgtcggta
      721 tcgccaaaac gcaaacgctc agccacaaag ccgagcgcca tcaatctgtc gattaaaatt
      781 tgttggcagc cctcatccaa gggcgtgact gatgcccttg ccatcagttg tttggtcaat
      841 tccaaggtgg tgttcgttgt catactcttc tattctttaa aagcaaaaag actgtttgca
      901 cagtctggta ttgtcatcgt tttcagactc agcttacctg atatcggctt attgtaaaag
      961 tctattaaaa ccatgtactc cacccagcgg cacaaaccat ggcaatatct caataaagac
     1021 acagttttca ccccaataaa aatccaaact gaataatgat gtggtcgcct aaaaagctgt
     1081 gccataataa ctgcttgctc aaatttacat ggcactggtc aggcatgcag tctttatttc
     1141 aaaacaccca ttctcgtttt ttcttgattt tgttgaccat gggcagcttt tctttgggca
     1201 tgaccgaata ctcgatgatt gggctcagca atgaaatcgc tgcagatttg aagttaacca
     1261 atcaccaaac cagccaagtg atgagtgcgt atgcaatggg cgtgttaatt ggcgctccac
     1321 ttttggccat cttgggctcc aaactcaatc gccacacatt gatggcctac ctgctgttgt
     1381 ggtgtgcttg tggcaacctt ttcatctcgt ttgcccattc ttatgaacaa ttgcgctggt
     1441 taagaaccct caatggattc ccgcatgggc tttatttcag taccgcagcc ttattgattt
     1501 atgatgtttt tcccaaaaac aaacgtgcca attacatcgg tgtgatgttc tcaggc
//
LOCUS       DAOMVK010000547         1556 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_23259_length_1556_cov_2.814124, whole genome shotgun sequence.
ACCESSION   DAOMVK010000547 DAOMVK010000000
VERSION     DAOMVK010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23259_length_1556_cov_2.814124"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="PK856_12625"
     CDS             complement(<1..1001)
                     /locus_tag="PK856_12625"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957627.1"
                     /note="catalyzes the conversion of citrate to isocitrate
                     and the conversion of 2-methylaconitate to
                     2-methylisocitrate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase B"
                     /protein_id="HRL03258.1"
                     /translation="MSFLDSYRAHVAERAALGTVPLPLSAQQTAELVELLKNPPAGEG
                     EFLVDLLTHRVPPGVDDAAKVKASFLAAVAEGTVQSPLVSAELATKLLGTMLGGYNIS
                     ALVELLDDDKLAATAAEGLKHTLLMFDAFHDVKTKMDNGNAYAKEVIESWSNAEWFTS
                     RPKVQEKITVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNPREGIVPDQPGS
                     VGPIKLLEELKAKGNLVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCL
                     GGKIAPIFFNTQEDSGALPIEVDVTNLNMGDVVDIFPYEGKIVKNGETIANFELKSQV
                     "
     gene            1364..>1556
                     /locus_tag="PK856_12630"
     CDS             1364..>1556
                     /locus_tag="PK856_12630"
                     /inference="COORDINATES: protein motif:HMM:NF013209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HRL03259.1"
                     /translation="MSNSEKMWQQAEKVTQVYKLLANTHRLMILCVLLEQDELCVGDL
                     NLHIPLNASPLSQHLKLLRD"
BASE COUNT          427 a          393 c          327 g          409 t
ORIGIN      
        1 acttgcgatt tcaattcgaa attggcaatg gtttcaccat ttttaacgat tttgccttcg
       61 taagggaaga tgtctaccac atcgcccatg ttcaaattgg tcacatccac ttcaattggc
      121 aacgcgcctg agtcttcttg ggtattgaag aaaatcgggg caattttgcc gcccaagcac
      181 acgccaccaa agcgtttgtt aggcacgaaa ggaatgtctt cacctgtgtg ccaaatcacc
      241 gagttggttg cagatttacg gctagagcca gtaccgacaa catcacccac gtaagccacc
      301 aaattgcctt tggctttcaa ctcttccaac aatttgattg gacccacaga acctggttgg
      361 tctggcacga tgccttcgcg tgggtttttc aacatggcca atgcatgcaa tggaatgtct
      421 ggacggctcc aagcatcagg tgctggagac aaatcgtcgg tattggtttc acccgttact
      481 ttgaacacgg taacggtgat tttttcttgt actttaggac gagaagtgaa ccattcggca
      541 ttagaccaag attcaatcac ttctttggca taagcattgc cattgtccat tttggttttg
      601 acatcatgga aggcatcaaa catcaacaaa gtgtgtttca agccttcggc tgcagtggca
      661 gccaatttgt cgtcatccaa caattcaacc aaagcggaaa tattgtaacc acccaacatg
      721 gtacccaaca atttggtggc caactcggcg cttaccaatg ggctttgtac tgtgccttca
      781 gccacagcag ccaagaaaga cgctttgact ttagcagcat cgtccacacc aggaggaacg
      841 cggtgggtca acaaatccac caagaattca ccctcaccgg ctggtgggtt tttcaagagt
      901 tcaaccaatt cagcagtctg ctgagctgac aaaggcagag gaacggttcc taaagcggcg
      961 cgctccgcca cgtgggcacg gtagctatcc aaaaaagaca tcagaagttt ccttttgttt
     1021 aaatataaac ggttcttatt cgtattctat gctgttgctt aacaacacat agattttaaa
     1081 tcaaattcac cggataataa accgctattc ttgcataaaa gactcaggct atcgcagtgg
     1141 tttaaacaga ttaaaactgc acacatcgtt tgcttgctgc aaccgtgttt taaaccatgc
     1201 aaaagccaag cattacaagc ggcctgtaaa cctctgtcca tctccgccct tctctcaaga
     1261 ctgacagcca caatttgtca accgacacac acttgtgtaa tgtggcttcg tctccatttt
     1321 aatcactgcc tgtcctcaca cgagacttac cgagcctaga aacatgagca attccgagaa
     1381 aatgtggcaa caagccgaaa aagtgaccca agtgtacaaa ctcttggcca atacccaccg
     1441 cctgatgatt ttgtgtgtat tgctcgagca agatgaattg tgcgtgggtg atttgaatct
     1501 acacattccg ctgaatgcct ccccgctgtc acagcactta aaattgctgc gtgacg
//
LOCUS       DAOMVK010000548         1550 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_23365_length_1550_cov_2.469565, whole genome shotgun sequence.
ACCESSION   DAOMVK010000548 DAOMVK010000000
VERSION     DAOMVK010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23365_length_1550_cov_2.469565"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="PK856_12635"
     CDS             complement(<1..352)
                     /locus_tag="PK856_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRL03260.1"
                     /translation="MAYQVLARKWRPKHFADLVGQEHVVGALRNALAQGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCEHPDQGEPCGVCAQCVQIDTGRHVDVVEIDAASNTGID
                     NIREVLENAQYAPSM"
     gene            complement(356..1423)
                     /gene="mltB"
                     /locus_tag="PK856_12640"
     CDS             complement(356..1423)
                     /gene="mltB"
                     /locus_tag="PK856_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HRL03261.1"
                     /translation="MKKLAILISLALLAACTPKTVEQTSTGVTVKPVKDQQPEIFSNV
                     TYPEGTTFLSRPDVQRYIDRKVATGQFSRAEMEAFFANVQNKPNIIGIMDKPGTSRPW
                     YEFRKNNVAGSRITGGVKYWAANDYAVTQASRMYGVPPEVLVAIVGIETNYGSNMGSF
                     RTADALVTLAFNYPRRAEFFRKELDEFMLMAHEERQDMFTFKGSYAGAMGMPQFMPSS
                     YRKWAVDANHDGHRNIWSDSNDVMASVANYMKQHGWKTGGKISVPVSFQMTEAIQAKL
                     DEDTQLTHTVGDFKRLGVQVSDASVADSEKAFLYRLETAPGQYDYYLGLNNFYAIWQY
                     NHSRLYVSAVKEIAENVARSR"
BASE COUNT          380 a          414 c          357 g          399 t
ORIGIN      
        1 ccatgctcgg tgcgtattgg gcgttttcca acacctcacg gatgttgtct atgcccgtat
       61 tggaagccgc atcgatttcc accacgtcca catggcggcc tgtatcgatt tgcacacatt
      121 gggcacatac gccacatggc tcaccttgat cgggatgttc acaattgagg cttttagcca
      181 gaatacgagc gatggtggtc ttgcccaccc cacgcgtacc tgttaacaaa taagcatggt
      241 gtaacctgcc ttgagccaag gcattgcgca gcgcccccac cacatgttct tgcccaacca
      301 aatcggcaaa gtgtttggga cgccatttgc gcgctaaaac ttgataagcc acagattaac
      361 gactccgcgc cacgttttca gcaatctctt tcactgctga cacgtacaag cggctgtggt
      421 tgtattgcca aatggcatag aaattattca ggcccaagta ataatcgtat tggcctggtg
      481 cggtttccaa gcggtataaa aaggcttttt cagaatcggc aaccgatgcg tctgacactt
      541 gcacacccaa gcgtttgaaa tcgcccacag tatgggttaa ttgcgtgtct tcatccaatt
      601 tcgcttggat ggcttcggtc atttggaagc ttactggcac agaaatcttg ccgcctgttt
      661 tccagccgtg ttgtttcatg taattggcca ccgacgccat cacatcattg gaatcgctcc
      721 aaatattgcg gtgaccatcg tgattggcat ccacagccca cttgcggtag ctggacggca
      781 tgaattgcgg catacccatc gcccctgcat aactgccctt aaaggtaaac atgtcttggc
      841 gctcttcgtg cgccatcaac atgaattcat ccaattcttt gcggaaaaat tcggcgcggc
      901 gtgggtagtt aaatgccaaa gtcaccaagg catcggctgt gcggaatgag cccatattgc
      961 tgccataatt ggtttctatg cccactatcg ccaccaaaac ttcaggtggt acaccataca
     1021 tgcggctcgc ttgagtcacg gcataatcgt tggcagccca atacttcaca ccacctgtga
     1081 tgcggctgcc tgccacattg ttcttgcgga attcatacca agggcgtgat gttcctggct
     1141 tgtccatgat gccgatgata ttgggcttgt tttgcacatt agcaaaaaaa gcctccatct
     1201 cagcacgaga aaactgtcct gtcgccactt tgcggtcaat gtaacgctgt acatcaggac
     1261 gactcaagaa agtggtgcct tcagggtaag tcacattgga aaaaatctca ggctgctggt
     1321 ctttcacggg ctttaccgtc acacctgtac tggtttgttc tactgttttg ggtgtacagg
     1381 ccgctaagag cgcaagcgag attaaaatag cgagtttttt catcatgaga tccccaatta
     1441 ttctaggcca ccagtgtacc aaaatctgcg cgcctaacaa acaacaaggc tgcctgaatc
     1501 atcaggcagc cttgaattca caacactaaa attacgcagc caaaggggca
//
LOCUS       DAOMVK010000549         1545 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_23467_length_1545_cov_2.556376, whole genome shotgun sequence.
ACCESSION   DAOMVK010000549 DAOMVK010000000
VERSION     DAOMVK010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23467_length_1545_cov_2.556376"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="PK856_12645"
     CDS             complement(<1..635)
                     /locus_tag="PK856_12645"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HRL03262.1"
                     /translation="MLKKPKCVLSYSGGKDSTLALYHMLQTAEPVALIAMLEEQGQRS
                     RSHGMDTDLIAAQAQALGLPIITAAASWQDYEAKFAEVLQSCQLQGITVLATGDLDVP
                     AVGSWHEQMAHAAGLELNMPLLQRPHQAVVDEFIDLGFVAIVVTVNLSLGMQPEDLGQ
                     RFDREFVQELQARGIDVAGEGGEFHTTVIDGPIFSQPLNVRQGERVYHGEYG"
     gene            complement(745..>1545)
                     /locus_tag="PK856_12650"
     CDS             complement(745..>1545)
                     /locus_tag="PK856_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HRL03263.1"
                     /translation="RVEAIVNQAVLANVAVDAAHMSFDDAIAAGAMALFGEKYGDEVR
                     VLKMGDFSTELCGGTHVSRTGDIGLFKIVSEQGIAAGVRRVEAVTGLNALAWAQEQEQ
                     LIKTVVAEVKAQTADDIVTKVQAAQSHAKQLEKALAEANSKLAAHAGSALLNEAQTVG
                     AARVIVKQMDGVDGNALRDITTDLTGKGDDLVVLLAAVADDKISLCAGVSKALSKTIK
                     AGDLVKTVAEQVGGKGGGRPDLAQAGGTDVAALPAALNGVVAWVQNKA"
BASE COUNT          350 a          497 c          334 g          364 t
ORIGIN      
        1 ccatactcgc catggtatac ccgttcgccc tgccgcacat tcaatggctg cgaaaatatc
       61 ggcccatcga taaccgtggt gtgaaattcg cccccttcgc ctgccacatc aatgccacgt
      121 gcttgcagct cttgcacaaa ttcgcggtca aatcgctgcc ccaaatcttc aggctgcatg
      181 cccaagctca aattcaccgt caccacaatc gccacaaaac ccaagtctat gaattcatcc
      241 accaccgctt ggtgcggccg ctgcaacaat ggcatgttca gctccagccc cgccgcatgc
      301 gccatttgct catgccaact gcccaccgca ggcacatcca aatcgcccgt tgccaatacg
      361 gtaatgcctt gcagctgaca gctttgcaat acctcggcaa acttggcctc atagtcttgc
      421 caactcgccg ccgccgtgat gatcggcaaa cccaaagcct gcgcttgtgc cgcaatcaag
      481 tcggtatcca tcccatgcga gcgcgaacgc tgcccctgct cttccaacat cgcaatcaaa
      541 gccacaggct ctgccgtttg caacatgtgg tacagcgcca aagtcgaatc cttgccacca
      601 ctgtacgaca acacacattt tggttttttc aacatctatt cctcacttca tactgagtat
      661 tgcacgccct tgcaatacag tgaaataccg acaaaaaagc ggcctgtaaa cacttacagg
      721 ccgcttttaa ctcaattgat tacattaagc tttgttttgt acccaagcca ccacaccgtt
      781 taatgcggca ggcaaggctg caacatcggt gccgccggct tgcgccaaat cagggcggcc
      841 gccgccttta ccaccgactt gctcggccac ggttttcacc aagtcgcctg ctttgatggt
      901 tttgctcaag gctttggaca cgcctgcgca caagctgatt ttgtcgtcgg caaccgcagc
      961 caacaacacc accaaatcgt cgcctttacc cgtcaaatcg gtggtgatgt cacgcaaagc
     1021 attgccatcc acgccatcca tttgtttgac gatgacgcgc gctgcgccca cggtttgtgc
     1081 ttcattcaac aaagcagaac ctgcgtgtgc ggccaatttg ctgttggctt cggccaaggc
     1141 tttttccaat tgtttggcat ggctttgagc ggcctgaact ttggtcacaa tgtcgtctgc
     1201 cgtttgggct ttgacttcgg ccaccacggt tttaatcaat tgctcttgct cttgtgccca
     1261 agccaatgcg ttcaaaccgg ttacggcttc cacgcggcgt acgcctgcgg caataccttg
     1321 ctcagacacg attttaaaca agccgatgtc gcctgtgcgc gatacgtggg taccgccaca
     1381 caattcggta gagaaatcgc ccattttcaa cacgcgcact tcgtcaccgt atttttcacc
     1441 aaacaaggcc atagcacctg cggcaatcgc gtcgtcaaac gacatgtgcg cagcatccac
     1501 cgccacattg gccaatacgg cttggttgac gatggcttca acacg
//
LOCUS       DAOMVK010000550         1541 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_23538_length_1541_cov_3.808883, whole genome shotgun sequence.
ACCESSION   DAOMVK010000550 DAOMVK010000000
VERSION     DAOMVK010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23538_length_1541_cov_3.808883"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..356)
                     /gene="rpsP"
                     /locus_tag="PK856_12655"
     CDS             complement(117..356)
                     /gene="rpsP"
                     /locus_tag="PK856_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRL03264.1"
                     /translation="MVVIRLSRGGSKHRPFYNVVVADSRNRRDGRFIERVGFYNPVAN
                     EKQERVRLNNDRLNHWVSQGAQLSDAVAKLVKEQA"
     gene            complement(446..>1541)
                     /locus_tag="PK856_12660"
     CDS             complement(446..>1541)
                     /locus_tag="PK856_12660"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRL03265.1"
                     /translation="FYKSNIGGVKLNIHDAPNLREAYRNIMERAAVLAPQAHINGITV
                     QPMLQQREAREVMISVTRDQVFGPVITFGAGGLAASIQKDSASSLPPLNNFIIQDMIK
                     RTKVGQTLGEFKNMRPIDTEALEQVLLRVSDMVSELPEIIELEINPLLLSPAGAIAVD
                     ARVVIDSHHVKSTRSQRYKHMAIMPYPSYLEEALQLKDGTKVTLRPLRPEDADMVQAF
                     VRNLSDESRYNRFMSSIKQLSQQVLVRFTQLDYDREMALALVYTDENGNEEVLGMSRY
                     VTDPDMEVCEFAISIADKWQGRGVGTILMQKLFDVASQQGLSTMRGEVLTSNSGMLQL
                     MKKLGFKLKKDPEDPNIYIVTRPLSADTAT"
BASE COUNT          388 a          369 c          374 g          410 t
ORIGIN      
        1 ttcggtatcg ctacctatct tgacccaacc tttaacgccc actgcaccac gtacaaaacc
       61 catggtgatt aatttgccgc catcgggcag ggtgaaaacc gcatcagaca tgtgaattaa
      121 gcttgttctt taaccaattt agcaaccgcg tcgctcaatt gagcaccttg gctaacccag
      181 tggttcaagc gatcgttgtt caaacgaaca cgttcttgtt tttcattggc aacagggttg
      241 taaaaaccaa cgcgctcaat gaaacggcca tcgcgacgat tgcgagaatc agcaacaacc
      301 acgttgtaga acggacggtg tttagaacca ccgcgagaca aacgaatgac aaccatgtgt
      361 aatccttaaa gttcaataga agtaaacccg caattttagg ctaaaatatg cttatctggc
      421 aagcgcttaa ttttacaggc cgcatttatg tggccgtatc cgcgctcagt gggcgggtaa
      481 caatgtaaat atttggatct tctgggtctt ttttcagctt gaaacccagt tttttcatca
      541 gctgtaacat gccgctgttg ctggttaata cctcgccacg catggtggac aagccttgtt
      601 gactggccac atcaaacaat ttttgcatca gtatggtgcc cacaccgcgt ccttgccatt
      661 tgtcggcgat ggatatggca aattcacaca cttccatgtc aggatcggtg acatagcggc
      721 tcatgcccaa tacttcttcg ttgccatttt catcggtata gaccaaagcc aaggccattt
      781 ctcggtcata atcgagctga gtgaagcgca ccaacacctg ttgcgacaat tgcttgatgc
      841 tggacataaa gcggttgtag cggctttcgt cagacagatt gcgcacaaat gcttgcacca
      901 tgtctgcatc ctctggccgt aacggacgca aggtgacttt ggttccatct tttaattgca
      961 aagcctcttc taaataagat gggtagggca tgatggccat gtgcttatag cgctgactgc
     1021 gggtggattt gacatggtgg ctgtcaatga ccacacgggc atctacagca attgcacccg
     1081 cagggctgag caataaagga ttgatttcca attcaatgat ttcgggcaat tcgctgacca
     1141 tgtcggacac gcgcagtaaa acctgctcca aggcttcggt gtctattgga cgcatgtttt
     1201 tgaactcacc caaagtctga ccaactttgg tgcgcttaat catgtcttga atgatgaagt
     1261 tattgagtgg aggcaaagaa gaggcgctgt ctttttgaat agaagctgct aagccgcctg
     1321 caccaaaggt aatgacgggg ccaaagactt gatcgcgggt gacgctgatc atcacttcac
     1381 gtgcctcacg ttgttgcagc atgggttgta cggtaatgcc attgatgtgg gcttgcggtg
     1441 ccaagacggc ggcccgctcc atgatgttgc ggtaggcttc gcgcaaattg ggtgcgtcgt
     1501 gtatattgag tttgaccccg ccaatattgg atttgtaaaa a
//
LOCUS       DAOMVK010000551         1537 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_23619_length_1537_cov_3.637652, whole genome shotgun sequence.
ACCESSION   DAOMVK010000551 DAOMVK010000000
VERSION     DAOMVK010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23619_length_1537_cov_3.637652"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="PK856_12665"
     CDS             complement(<1..656)
                     /locus_tag="PK856_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endopeptidase"
                     /protein_id="HRL03266.1"
                     /translation="MRKKQRTSSNRLPEKWFRRGLWLVAFVFAGFLIGLGGKIVGDLP
                     QAAPYKELREYVNHTTYDPLLLEQTRLQVRQTSLQEQSEQASLALEKQRNIRQSEQTS
                     LDNWLATRSATEQSDQNPEVIARTQKLDALKIEERKAQQHSEGIQQQQLDSNQRLAKV
                     NTELAQLDADAQVLKDADDRKIELRVFLYRLALTLPLLLIATYLFMKQRGSRWWPFVW
                     G"
     gene            complement(798..>1537)
                     /locus_tag="PK856_12670"
     CDS             complement(798..>1537)
                     /locus_tag="PK856_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HRL03267.1"
                     /translation="GAAMPSDVFIPILIATFFSTLAGLLTVAWCQKINLWDKTILAYL
                     GGLTAAIGGALYYFSSLEPAQMQIVSRVVGNFVLFGLFVSFIALAFYKRINVYDAFVE
                     GAKEGFQVAVNIIPYLVAILVAIAVFRASGTMDYLVGAIAWVVSALGFDTRFVEALPV
                     ALMKPLSGSGARGLMVELMQTNGADDFAARVACVIQGSTETTFYVLAVYFGSVAIKKT
                     RHAVPCALLAELAGVIAAICVAYVFWG"
BASE COUNT          417 a          438 c          346 g          336 t
ORIGIN      
        1 ccccacacaa acggccacca gcgtgagccg cgctgcttca tgaacaagta ggtcgcaatc
       61 aacaacagtg gcaaggtcaa ggccaagcgg taaaggaaca cccgcagctc gatttttctg
      121 tcatcggcgt ctttgagcac ttgcgcatct gcatccaatt gtgccaattc cgtgttgacc
      181 ttggccaaac gctgattact gtctaattgc tgctgttgta taccttctga gtgctgctga
      241 gcttttcgct cctcaatttt taaggcatcg agtttttgag tacgggcaat cacctcagga
      301 ttttggtccg attgctcggt ggctgaacgt gtggccagcc aattgtccaa gctggtttgc
      361 tcagactgcc tgatattacg ctgtttctcc aaggccaaac tggcttgctc gctttgttct
      421 tgcaagcttg tctgcctgac ttgtaaacgt gtttgctcca gcaacaaggg gtcgtatgtg
      481 gtgtgattca catactcacg caattctttg tatggtgcgg cttgcggtaa atcaccgaca
      541 attttgccgc ccaaaccgat taagaaacct gcaaacacaa acgccaccag ccacaaaccg
      601 cgccgaaacc atttttcggg caagcgattg gacgaggttc tctgtttttt acgcatggcc
      661 gtgactcctg tcattgaatg tgcccacagt ctaaccgaac gctctgagtt tgcagtgcgc
      721 aaacgcagaa gtagcgtttt gcgccacttc aaaccagact ttttaaatca ccgcgaccag
      781 cggcctgtaa attgctatta gccccaaaac acataagcca cacaaatggc ggcaatgaca
      841 ccagccagct cagccaacag cgcacatggc acggcatggc gggttttctt aatcgccacc
      901 gagccaaaat acacggccaa cacataaaac gtggtttcgg tagagccttg aatcacgcaa
      961 gccacgcgcg cggcaaaatc gtctgcgcca ttggtttgca tcaattccac catcaaacca
     1021 cgcgcacccg agccgcttaa aggcttcatc aaagccacag gcaaggcttc gacaaatcgg
     1081 gtatcaaaac ccaaagccga aaccacccac gcaatcgccc ccaccaaata gtccatagtg
     1141 cccgaggcgc ggaacacggc gatggccact aagatggcca ccaaatacgg aatgatgttc
     1201 accgccactt gaaagccttc tttcgcgccc tcaacaaagg catcgtacac attgatgcgc
     1261 ttgtaaaacg ccaacgcaat aaaagaaaca aacaaaccga acaacacaaa attacccacc
     1321 acgcgcgaca cgatttgcat ttgtgcaggc tctaggctgg aaaaataata cagcgcgcca
     1381 ccgatggccg cggtcaagcc gcccaaatac gccaagatgg ttttgtccca caaattgatt
     1441 ttttgacacc aagccacggt gagtaaaccc gccaaagtag agaaaaatgt ggcgattaaa
     1501 atggggatga atacgtctga aggcatcgcc gcacctg
//
LOCUS       DAOMVK010000552         1537 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_23623_length_1537_cov_2.740891, whole genome shotgun sequence.
ACCESSION   DAOMVK010000552 DAOMVK010000000
VERSION     DAOMVK010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23623_length_1537_cov_2.740891"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PK856_12675"
     CDS             <1..330
                     /locus_tag="PK856_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRL03268.1"
                     /translation="DEKAINLSYGHQRRLEIARALACEPKLLALDEPAAGMNPSETEG
                     LKHLMSKIRDDGVTILLIEHDVKLMMELCDRIAVLDYGQKIAEGLPEEIRTNPRVIEA
                     YLGASNE"
     gene            327..1037
                     /locus_tag="PK856_12680"
     CDS             327..1037
                     /locus_tag="PK856_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL03269.1"
                     /translation="MSLLKLENVNVNYGGIQAVSDLNLHIEQGELVTLIGANGAGKTT
                     TLRTITGLVKPKSGTLLYQDQDIRKTEVHKFVSQGLVMVPEGRGIFPKLTVLENLDMG
                     AFYRNDKAQIQADIEYVMTLFPRLKERAKQLAGTLSGGEQQMVAMGRAILSKPKLLLL
                     DEPSMGLAPIIVQQIFDIIQTVHKEGVTILLVEQNAKLALQISNRGYVMESGKITMAD
                     KADVLLNDPRVLSAYLGG"
     gene            1195..>1537
                     /locus_tag="PK856_12685"
     CDS             1195..>1537
                     /locus_tag="PK856_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRL03270.1"
                     /translation="MKRHEALIPFSQQHHHALVLVLKIQQDRWLDLDWEHEMQLLLSH
                     FDEEEALFQSCWLPLNQTELQQRFEAEHQQLRLMAIKRPVNCEEAKAFAILLKQHIRF
                     EEQILFTAFQTA"
BASE COUNT          437 a          311 c          379 g          410 t
ORIGIN      
        1 gatgaaaaag ccatcaattt gtcttatggt caccaacgcc gcttggaaat cgcccgtgct
       61 ttggcctgtg agcctaagtt attggctttg gacgaacctg cagcgggtat gaacccatct
      121 gaaaccgaag gcttgaagca cttgatgagc aaaatccgcg atgacggtgt gaccatcttg
      181 ttgattgagc acgatgtgaa attgatgatg gagttgtgcg accgcattgc cgtattggac
      241 tatggtcaaa aaatcgccga gggtttgcct gaggaaatcc gcaccaatcc acgtgtgatt
      301 gaagcctatt tgggagcctc aaacgaatga gcttattaaa acttgaaaat gtgaatgtca
      361 actacggtgg cattcaggcc gtgtctgatt tgaatttgca cattgagcaa ggcgaattgg
      421 tgactttgat tggtgccaat ggtgcgggta aaactactac gttgcgcacc atcacgggct
      481 tggtgaaacc caaatcgggc actttgctgt accaagacca agacatacgc aaaaccgaag
      541 tgcacaaatt tgtcagccaa ggcttggtga tggtacccga aggtcgcggt attttcccta
      601 agctgacggt attggaaaac ttggacatgg gcgcatttta ccgcaatgac aaggcgcaaa
      661 tacaggccga cattgaatat gtgatgactt tgttccctcg cttaaaagag cgtgccaagc
      721 aattggcagg cactttgtcg ggcggtgagc agcaaatggt ggccatgggt cgtgcgattt
      781 tgtccaaacc caaattattg ttattagatg agccgtctat gggtttggca ccgattatcg
      841 tgcagcaaat ctttgacatc atccaaaccg tgcacaaaga aggtgtgacc atcttgttgg
      901 tagagcaaaa tgcgaaactg gctttgcaaa tttccaatcg tggttacgtg atggaaagtg
      961 gcaaaatcac catggcagat aaagccgatg tgttgctcaa tgatccacgt gtattgagcg
     1021 cttatttggg cggttaagtg acttgtttaa aaaaagcgac tgataggtcg cttttttttg
     1081 ttgtacatca attggctggg acttatgtgt gagagtcaat ccataattaa atacaattga
     1141 tatgaaagaa ccaaactcaa tattcaaatt tttaattgtg aggactatgt caccatgaaa
     1201 cggcacgaag ccttgattcc attttcacag caacaccacc atgccttggt tttggtgctg
     1261 aaaatacagc aagatcgctg gctggatttg gattgggaac atgagatgca gttattatta
     1321 agccatttcg atgaagaaga agccttgttt caaagctgct ggttgccatt gaaccaaaca
     1381 gaattgcagc agcgatttga ggctgaacac caacaattgc gactcatggc tattaagcgg
     1441 cctgtaaact gtgaagaagc aaaggcattt gccattttat tgaaacagca cattcgtttt
     1501 gaagaacaaa tcctgtttac agctttccag accgcaa
//
LOCUS       DAOMVK010000553         1537 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_23624_length_1537_cov_2.713225, whole genome shotgun sequence.
ACCESSION   DAOMVK010000553 DAOMVK010000000
VERSION     DAOMVK010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23624_length_1537_cov_2.713225"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1537
                     /locus_tag="PK856_12690"
     CDS             <1..>1537
                     /locus_tag="PK856_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03271.1"
                     /translation="CKHILAAVLHLQQHMADAVDTAVCDTPSEVAAEADTVAAATTVA
                     TEVPMSTSNQITSALPAWLAPYQHLDEAALSTWGNPGLLRRANKAVEAGEVTLLEIGS
                     EQGQWQNDDALVTLNAQGLTQAACNCPAAGACKHILVAVLWVQKALQDGSETANTLTE
                     NTEASQTANHAPTGLQAALSEALDWERLNVGKIGKVHWRQAYAVWAVWQQGLPPSTEV
                     QAAKILFHTDLSSDAVVYLLGGGFAGMLSSIEKKRQVPVHLALIAQLWQEQQQSFPWP
                     EEVLPDDNADASRLGEDERSLLLQLQTACQRLLQLGLNHLMASEAQQWQWLQLSARVE
                     RLPRLAGMLRRLAGEVRQLAENHILSDSNDTLLSVARLAAYIEALLHADGERLPSLRG
                     VLRRTYQTQDEAMTVLPLGVNWWQGEGGASGLNVYVWSVEEAAIMTTVQARGNANDRS
                     FNRYIAWNGAGFWNRSAEQIMGHQITLRAPRVLGDRLANQVAVPSQAQSLAPRFFEPS
                     LTS"
BASE COUNT          338 a          397 c          480 g          322 t
ORIGIN      
        1 catgcaaaca catcttggcg gcggtattgc atttgcagca acacatggcg gatgcagtgg
       61 acacagccgt ttgtgataca cccagtgagg ttgcggccga ggcagacact gtggctgctg
      121 cgacaactgt ggctaccgaa gtgcccatgt ccacatcaaa ccaaatcaca tctgccttgc
      181 ccgcatggct tgccccatac caacacttgg acgaggccgc tctcagcact tggggtaatc
      241 ctggtttgct caggcgcgcc aacaaagccg tagaggcagg cgaagtcacg ctgctggaaa
      301 tcggctctga acaaggacag tggcaaaacg acgatgcgct tgttacgttg aacgcgcaag
      361 gcttgaccca agcggcctgt aactgccccg cagcaggtgc gtgcaaacac attttggtag
      421 cggtgttgtg ggtgcagaaa gccttgcaag acggcagtga aacagcgaac acgcttacag
      481 aaaataccga ggcatcgcaa acggcaaatc atgctccaac gggtttacag gccgcattga
      541 gcgaggcttt ggattgggag cgtttaaatg tcggcaaaat tggcaaagtg cattggcgcc
      601 aagcctatgc ggtgtgggcc gtttggcaac aaggcctgcc gccgagcaca gaggtgcaag
      661 cggcgaagat tttgtttcac accgacttga gcagcgatgc ggtggtgtat ttgcttggcg
      721 gtggttttgc gggcatgttg tccagcatag agaaaaagcg ccaagtgcct gtgcatctgg
      781 ccttaatcgc ccaattgtgg caagagcaac agcaaagctt tccgtggcca gaagaggtgt
      841 tgcctgacga caatgccgat gccagccgct tgggtgaaga cgagcgcagt ttgctgctgc
      901 aattgcaaac cgcttgtcag cgcttgttgc aattgggctt gaaccacttg atggcaagcg
      961 aagcgcagca atggcaatgg ctgcaattgt cggcgcgggt agagcgtttg ccgcgcttgg
     1021 cgggcatgct caggcgcttg gcaggcgaag tgcgccaact ggctgagaat cacatcttga
     1081 gcgacagcaa cgataccttg ttgtcggtgg cgcgtttggc tgcctacata gaagcgctgt
     1141 tgcatgccga tggcgagcgt ttgcccagtt tgcgtggcgt gttgcgccgc acttaccaaa
     1201 cccaagacga ggcgatgacg gtattgccct tgggtgtgaa ttggtggcag ggcgaaggtg
     1261 gggcttcggg gctgaatgtg tatgtgtgga gcgtggaaga ggccgccatc atgaccacgg
     1321 tgcaagcgcg tggcaatgcc aacgaccgca gcttcaatcg ttacatcgct tggaatggcg
     1381 caggcttttg gaaccgcagc gccgagcaaa tcatgggcca tcaaatcacc ttgcgcgcgc
     1441 cgcgcgtgtt gggcgaccgc ttggccaatc aagtggctgt gccttcgcaa gcgcaaagtc
     1501 ttgcgccgcg cttttttgag ccttcgctca cgtcgta
//
LOCUS       DAOMVK010000554         1536 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_23644_length_1536_cov_2.345037, whole genome shotgun sequence.
ACCESSION   DAOMVK010000554 DAOMVK010000000
VERSION     DAOMVK010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23644_length_1536_cov_2.345037"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="PK856_12695"
     CDS             complement(<1..973)
                     /locus_tag="PK856_12695"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRL03272.1"
                     /translation="MLGHEHQSMELGLKKTGLTLGKFAPLHKGHQYLIETALAEMDEV
                     VVMIYATEVTEIPLNVRAAWLRDLYPSITVIECWDNPSDTVSNEHEHEIREEDYIVHK
                     LAGRHISHFYCSEYYGEHVSRRLNAEDRRVDEARQQVPISATQIRAQPYHYRKFVHPR
                     VYWDMLIKCVFVGTVSAGKTTLVEALAAHQTTVGVPEYGRVYWAEHQQNRRLQIEELD
                     TIALTHQQWEYELAHEAEDVVFVDTNALTTRLFCIDYHGKSTPTIEALAHANASRYDL
                     FFMCQDDIPYDDTWDRSGPQKREVLQQQIRADLARRKIPYHDIRGSVA"
BASE COUNT          386 a          447 c          324 g          379 t
ORIGIN      
        1 ctgccacact gccgcgtatg tcatggtatg gaattttgcg ccgcgccaaa tcggcacgaa
       61 tttgctgctg caatacttcg cgcttttgtg gcccacttct gtcccatgta tcgtcatacg
      121 gtatgtcgtc ctggcacatg aaaaacaagt cgtaacggct ggcattggca tgggccaagg
      181 cctcaatcgt gggcgtagat ttgccgtggt aatcaatgca gaacaagcgc gtggtcaagg
      241 cattggtgtc cacaaacacc acatcttcgg cttcgtgcgc cagctcatat tcccattgtt
      301 ggtgggttaa ggcgatggtg tccaattcct caatctgcaa gcggcggttt tgttggtgct
      361 cggcccaata cacgcgccca tattcgggca cacctactgt ggtttggtgc gccgccagag
      421 cttctaccag cgtggttttt cctgccgaca ccgtgcccac aaacacgcat ttgatgagca
      481 tgtcccaata cacacgcggg tgcacaaatt tgcggtaatg atagggctga gcgcgtatct
      541 gtgtggccga aatcggcact tgctgacgcg cttcatccac acgcctgtct tccgcattca
      601 aacggcggct cacatgctca ccataatact cgctgcaata aaaatggctg atgtgccgcc
      661 ctgccaattt gtgcacaatg taatcttctt cacggatttc atgctcgtgc tcattggaaa
      721 cggtgtcgct cggattgtcc caacactcaa tcaccgtgat gctgggatac aagtcacgca
      781 gccacgctgc ccgcacattc agcggaatct ctgtgacttc agtggcataa atcatcacca
      841 ccacttcatc catttccgcc aaagccgttt caatcaaata ctgatgcccc ttgtgcaagg
      901 gagcaaattt ccccaaggtc aaacctgttt ttttcaaacc caactccatg gactgatgtt
      961 cgtgaccaag cattacaggc cgcttatgtg ttgcgaagtg ctggctatca ttgtctaagc
     1021 aaagccttaa ggccaacacc atcaagatac tcaggcctgc aacaataccc tagcaccatg
     1081 acttacgcaa accacgccag caaacgagca agtccatggt cgctgcacca actttcggct
     1141 aggctaacca gccaaagaaa cactgatact ccgcttacaa tggtttgcat gctcatacaa
     1201 ttgttactgc cttagcgtta cacagttcac cacaggtata atgctctcgc ttagccagtg
     1261 gttgtgctta ttccgcgcca ctcattgcag agttaaaaca tcagttttgc ccaacccaaa
     1321 acaatttact acattttcca gcagcctatc tcacatacac aaaggtttct atgccgtcca
     1381 ttcaacacct caccgaacgt ctgcaacaat tggccaagct agaccccaca tttcaagctt
     1441 ttggcacggc agagcatcaa taccaactca acccttgctt gaatatccaa gccatacgcg
     1501 caatagaaaa ccaatatgcc atccaactac ctgaag
//
LOCUS       DAOMVK010000555         1526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_23828_length_1526_cov_2.229096, whole genome shotgun sequence.
ACCESSION   DAOMVK010000555 DAOMVK010000000
VERSION     DAOMVK010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23828_length_1526_cov_2.229096"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PK856_12700"
     CDS             <1..300
                     /locus_tag="PK856_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03273.1"
                     /translation="GVVMSMDFENDFFTAMIEAIFGAVGGIAFFSTFVYFSLSGGLIA
                     GILGAMTIGLVLALALGFAASLVFVIVYGIVKLCQLGRHITARHKQNTIKKATQV"
     gene            complement(512..>1526)
                     /locus_tag="PK856_12705"
     CDS             complement(512..>1526)
                     /locus_tag="PK856_12705"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRL03274.1"
                     /translation="TIGLLAMALKNIGLDAKSYTGWQVAVTTDNSHTKARIENIDADH
                     IHADIKEGKVIIVAGFQGIDGHGDITTLGRGGSDTSAVAMAAALKADECQIYTDVDGV
                     YTTDPRVVPEARRLKTISFEEMLELASLGSKVLQIRSVEFAGKYKVRLRVLSSLQEGG
                     EGTLITFEEDEDMEKAAVAGIAFDKNQARINVRGVPDKPGIAYQILGAVADANIEVDM
                     IIQNVGTEGTTDFSFTVPRGDYQRTLNILNEVQKNIGAQIVDGDNTVCKVSIVGVGMR
                     SHVGVASTMFRTLAEEGINIQMISTSEIKVSVLIDEKYLELATRVLHSVFGLDDPSKV
                     AQA"
BASE COUNT          342 a          365 c          376 g          443 t
ORIGIN      
        1 ggagtggtga tgagcatgga ctttgaaaat gactttttta ctgcgatgat cgaggcaatt
       61 tttggcgccg taggaggcat tgcctttttc agcacttttg tctatttctc attgagtggt
      121 ggtttgattg cgggcatttt gggcgccatg accatcggtt tggtattggc cttggctttg
      181 ggttttgctg ccagcttggt gtttgtcatt gtgtacggta ttgtgaaatt gtgccagctt
      241 ggtcgacaca tcactgcacg acacaaacaa aacaccatta aaaaagcgac ccaagtttag
      301 accgcttttt ctttcatacg gtgtaacacg cttacaaggg cttaatcccg ctaaatccaa
      361 atctatccaa acccatctgt ctgggactaa aaaatgcctt ttgaaacaac aaacattgtg
      421 atggccagat tgcttcttta atttaaaaca cccataaaaa agcggcctgt aaaccataca
      481 ggccgcttta tttactgaga tgtgacagtg cttacgcttg agccactttg cttggatcgt
      541 ctaagccgaa cacgctgtgc aacacgcggg tggccaattc caagtatttc tcatcaatca
      601 ataccgatac tttgatttca gaagtggaaa tcatttggat gttgatgcct tcttcagcca
      661 aagtgcggaa catggtggaa gccacgccca cgtgtgagcg catgcccacg ccgacgatag
      721 acactttgca cacggtgttg tcgccatcaa cgatttgcgc gccgatgttc ttttgaactt
      781 cgttcaaaat gttcaaagtg cgttggtaat cgccacgtgg tacggtgaat gagaagtctg
      841 tagtgccttc tgtgcccacg ttttggatga tcatgtccac ttcgatgttg gcatcagcca
      901 ctgcgcccaa aatttggtaa gcaatgcctg gcttgtcagg tacgccgcgt acgttgatgc
      961 gtgcttggtt tttatcgaag gcgatgcctg ccactgctgc tttttccatg tcttcgtcct
     1021 cttcaaaggt aatcagggtg ccttcgccac cttcttgtaa actgctcaat acgcgcaagc
     1081 gcactttgta tttgccggca aattcaaccg aacggatttg caaaactttt gagcccaagc
     1141 tcgccaattc caacatttct tcgaaagaaa tggttttcaa gcggcgtgct tcaggcacca
     1201 cgcgtgggtc ggtggtgtaa acgccatcaa cgtcggtgta gatttggcat tcgtcggctt
     1261 tcaatgccgc tgccatcgct accgctgatg tgtctgagcc gccacggccc aaagtggtga
     1321 tgtcgccatg gccgtctatg ccttggaaac ctgccacgat gatgactttg ccttctttga
     1381 tgtcggcatg aatgtggtcg gcatcaatgt tttcgatgcg ggctttggtg tgtgaattgt
     1441 cggtggtgac agcgacttgc cagcctgtgt agctcttggc atccaaaccg atgtttttca
     1501 aggccatggc gagcaagcct atggtc
//
LOCUS       DAOMVK010000556         1526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_23829_length_1526_cov_2.127124, whole genome shotgun sequence.
ACCESSION   DAOMVK010000556 DAOMVK010000000
VERSION     DAOMVK010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23829_length_1526_cov_2.127124"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /gene="rpoE"
                     /locus_tag="PK856_12710"
     CDS             <1..557
                     /gene="rpoE"
                     /locus_tag="PK856_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HRL03275.1"
                     /translation="QGDTKAFEMLVVKYNRRLGRLLSRFIKDEHEVNDVVQESFIKAY
                     RALPNFRGDSAFYTWLYRISINTAKNFLANNNKRPIMASEITNGDGEVMDVVEQVADN
                     LTPEAELMNKQILQTVESAVAKLPEDLRRAITLREMDGLSYEEIAEVMQCPIGTVRSR
                     IFRAREVIAADLRPLLDTSENNRW"
     gene            723..>1526
                     /gene="dapF"
                     /locus_tag="PK856_12715"
     CDS             723..>1526
                     /gene="dapF"
                     /locus_tag="PK856_12715"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRL03276.1"
                     /translation="MTTLNFTKMHGLGNDFIVINALNQPVEWTADNIKSWSDRHTGIG
                     FDQLLLIEASENERCDFKYRIFNADGGEVEQCGNGARCFAKYVTEHGLTDKTSVMVET
                     MRGIILLNVLADGQITVDMGKPHFAPEEIPFIPKNKAEHDAKVHMLVVGMETVPVSLV
                     SMGNPHAVMLVEDVATAPVQVLGEAIEKHPQFPQRVNVGFGQVISPSEINLRVFERGV
                     GETQACGTGACAAVVAGISHGLLSETVLVHLPGGDLTIHWKEGESVLMTG"
BASE COUNT          383 a          350 c          435 g          358 t
ORIGIN      
        1 agcaaggtga caccaaggca tttgaaatgc tggtggtgaa atacaaccgt cgcttgggtc
       61 gcttgttatc acgctttatc aaagatgagc atgaggtaaa cgatgtggtg caagaatcgt
      121 ttatcaaggc ttatcgcgcc ttgccgaatt ttcgtggtga cagcgctttt tacacttggc
      181 tttaccgcat cagcatcaac acggccaaaa attttctggc caacaacaat aaacgcccca
      241 tcatggccag cgaaatcacc aatggcgatg gtgaggtgat ggatgtggtg gaacaggtgg
      301 ccgataattt aacccctgaa gcggagttga tgaacaagca aattttgcaa accgtcgaaa
      361 gcgcggtggc aaaattgcca gaggatttgc gccgagcgat taccttgcgt gagatggatg
      421 gcttgtcgta tgaggaaatt gccgaggtga tgcagtgtcc aatcggcacg gtgcgctcgc
      481 gcattttccg tgcacgtgaa gtgattgcgg cggatttgcg accattgttg gatacgagcg
      541 aaaacaaccg ctggtgagtg atttgaatgt ttacaggccg cttgtgtgat gatacaagcg
      601 gcctgtaaac atttatgaca aagataaagc cgcaatcctt gcgaataccg ccacagacac
      661 tgtgctggcg tggcaattta tgccacaatg aaagccttct gttttggcct acggttaatc
      721 ccatgacaac tttgaatttc accaaaatgc acggtttggg caacgatttt atcgtcatca
      781 atgccttgaa tcagcccgtt gaatggacgg ctgacaacat caaatcgtgg tcagaccgcc
      841 atacaggcat aggctttgac caattgttgc tcatcgaggc ttccgaaaac gagcgctgtg
      901 atttcaaata ccgcattttc aatgccgatg gcggcgaagt agagcagtgt ggcaatggtg
      961 cgcgttgttt tgccaagtac gtgaccgagc acggcttaac cgacaaaacc tcggtgatgg
     1021 tggaaaccat gcgtggcatc attctgctga acgtgttggc cgatggccaa atcaccgtgg
     1081 acatgggcaa gccgcatttt gcgccagaag agattccctt tattcccaaa aacaaagccg
     1141 aacacgatgc caaagtgcac atgctggtgg tgggcatgga aaccgtgccc gtgagcttgg
     1201 tgagcatggg caatccgcat gcggtgatgt tggtggaaga cgtggccaca gcgccggtgc
     1261 aagtgttggg cgaggccatt gaaaaacacc cacagtttcc gcaacgcgtg aatgtgggtt
     1321 ttggccaagt catttcgcct tccgaaatca atttgcgcgt gtttgagcgc ggcgtgggcg
     1381 aaacccaagc ctgcggcaca ggcgcgtgtg cggcggtggt ggctggcatc agccatggct
     1441 tattgagcga aaccgtgttg gtacacctgc caggcgggga tttgaccatt cattggaaag
     1501 agggcgaatc ggtattgatg acaggc
//
LOCUS       DAOMVK010000557         1519 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_23970_length_1519_cov_3.017760, whole genome shotgun sequence.
ACCESSION   DAOMVK010000557 DAOMVK010000000
VERSION     DAOMVK010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23970_length_1519_cov_3.017760"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="PK856_12720"
     CDS             <1..312
                     /locus_tag="PK856_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL03277.1"
                     /translation="HTRSHIKHLKAQLANLSESEQTIHLLHLKWLRSLNTRAILENLP
                     TPQCHIWGDVDALIPKHMTDALALPPHAHTHIVTGASHLLPLTHSTECINTITQWLDA
                     Q"
     gene            326..>1519
                     /gene="bioA"
                     /locus_tag="PK856_12725"
     CDS             326..>1519
                     /gene="bioA"
                     /locus_tag="PK856_12725"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009119913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HRL03278.1"
                     /translation="MHTPSFDRDHLWHPYTSVTQPLPTFHVKRAEGVTIELASGEHLI
                     DGMSSWWCALHGYNHPRLNAAVSSQLQDMAHIMFGGLTHTPAIELGKILLQMAPPSMD
                     KIFYADSGSVAVEVALKMAIQYQHSRKRPVKTNVATVRSGYHGDTWNAMSVCDPVTGM
                     HHIFGSSLPQRHFVPAPLSRFDGAWDAQDITPVRELFAAEHDNLAAFIIEPIVQGAGG
                     MFFYHPQYLRELRCLCDEFDILLIFDEIATGFGRTGKLFAWEHAGVEPDIMCVGKALT
                     GGYMTLSAVLTNRHVAQTIGEGEAGVFMHGPTFMANPLACAVARASCELLLEQDWQAN
                     IERIQTQLHGLQAASSWSHVADVRVLGAIGVIELKHPVNMAQIQALIVAHGVWLRPFG
                     RLIYLM"
BASE COUNT          355 a          463 c          387 g          314 t
ORIGIN      
        1 cacacccgat cccacatcaa acacctcaaa gcccagcttg cgaacctcag cgaatcagaa
       61 cagaccatac atttgctgca tttgaagtgg ctgcgcagcc tcaatacccg cgccatttta
      121 gaaaatctgc ccacgccgca atgccacatt tggggcgatg ttgatgcctt aattcccaaa
      181 cacatgactg acgcactggc attaccacct cacgcacaca cgcacatcgt cacaggtgcc
      241 agccatttgc tgccactgac gcacagcact gaatgcatca ataccatcac tcagtggctg
      301 gatgcccagt aaagaaagac ccatcatgca cacacccagc ttcgaccgcg accatttgtg
      361 gcacccttac acctcagtca cccagccctt gcccacgttt catgtcaaac gcgccgaggg
      421 cgtgaccata gaattggcgt caggcgaaca cctgatagac ggcatgtcat cgtggtggtg
      481 cgcgctgcat ggctacaatc acccgcgcct caatgccgcc gtcagcagcc aattgcaaga
      541 catggcccac atcatgtttg gcgggctcac ccacacgccc gcgatagaac tgggcaaaat
      601 tttgttgcaa atggcgccgc cgtccatgga caagattttc tacgccgact caggctcggt
      661 ggcggtagaa gtcgcgttga aaatggcgat tcaataccaa cacagccgca agcggcctgt
      721 aaaaaccaat gttgccaccg tccgaagcgg ctaccacggc gacacttgga acgcgatgtc
      781 agtgtgcgac cctgttacgg gcatgcacca catttttggc agcagcctgc cacaacgcca
      841 ttttgtgccc gcaccgctca gccgttttga tggcgcatgg gatgcgcaag acatcacacc
      901 agtgcgcgaa ctcttcgccg ccgaacacga caaccttgcc gcgttcatca tcgaacccat
      961 cgtacaaggc gcgggcggca tgtttttcta ccacccacaa tacctgcgcg aattgcgctg
     1021 tttgtgcgac gaattcgaca ttttgctgat tttcgatgaa attgccacgg gctttggtcg
     1081 cacaggcaag ttgtttgctt gggaacacgc gggcgtcgag cccgacatca tgtgtgtggg
     1141 caaagccctc acaggcggct acatgacgct gtcggccgtg ctcaccaatc gccatgtggc
     1201 gcaaaccata ggcgaaggcg aagcgggtgt gttcatgcac ggccccacgt tcatggccaa
     1261 tccgcttgcg tgcgcagtgg cgcgggcatc ttgcgaattg ctgctggagc aagattggca
     1321 agccaacata gagcgtattc aaacccaatt acacggttta caggccgcat caagctggtc
     1381 gcatgtcgcc gatgtgcgcg tgctcggagc catcggtgtg attgagttga aacaccctgt
     1441 aaacatggcg caaatacaag ctctcattgt ggcgcatggt gtgtggctca gaccgtttgg
     1501 gcgcttgatt tacctgatg
//
LOCUS       DAOMVK010000558         1518 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_23996_length_1518_cov_1.875598, whole genome shotgun sequence.
ACCESSION   DAOMVK010000558 DAOMVK010000000
VERSION     DAOMVK010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23996_length_1518_cov_1.875598"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /gene="phhA"
                     /locus_tag="PK856_12730"
     CDS             complement(<1..423)
                     /gene="phhA"
                     /locus_tag="PK856_12730"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958084.1"
                     /note="phenylalanine 4-hydroxylase; phenylalanine
                     4-hydroxylase; catalyzes the formation of
                     4a-hydroxytetrahydrobiopterin and tyrosine from
                     phenylalanine and tetrahydrobiopterin; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HRL03279.1"
                     /translation="MDEPFVYTARQSDEHGHINWSESEDHTWHTLITRQWGNLQDKAC
                     DEYLQGLERLNLPHDHIPALKDVSQVLYHATGWQCAEVPALISFDRFFKLLSERKFPV
                     ATFIRRPQDMDYLQEPDIFHEIFGHCAMLTHPAFADFTA"
     gene            complement(763..1392)
                     /locus_tag="PK856_12735"
     CDS             complement(763..1392)
                     /locus_tag="PK856_12735"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRL03280.1"
                     /translation="MEPLDSIYHSTQVEVGSSGDEDEVNLASAAQRIAAWIINRGIEI
                     VLMIPAIVLIVVLIMQNKNGLDDLENMESFFTSMMGGIGLVMVLYLIYGGIQLYFMCK
                     YGQSIGKKLMKIRVVRENGDMAGFVHNVLLREVAYGLICSVIMMVLMGIFVAVFGVEA
                     NSGVAMVIDVLLQIVSYLPTLACFIMLFMESRNRQTLQDLLAKTYVVQV"
BASE COUNT          436 a          400 c          309 g          373 t
ORIGIN      
        1 cgcagtaaag tcggcaaacg cggggtgggt aagcatggcg cagtggccaa aaatctcatg
       61 aaaaatgtct ggctcttgca aataatccat gtcttgcggg cggcgaatga aggtcgccac
      121 cggaaatttg cgctcagaca agagcttaaa aaagcggtcg aaactgatta aggccggcac
      181 ttcagcgcat tgccaaccgg tggcatggta caaaacctga ctgacgtctt ttaaagcagg
      241 aatgtggtcg tgcggcaaat tcaagcgctc caagccttgc aaatactcat cgcaagcctt
      301 gtcctgcaag ttgccccatt gccgtgtaat caatgtgtgc caagtgtggt cttcgctttc
      361 agaccaattg atgtgaccgt gttcatcgct ttgccgggcg gtgtagacaa atggctcatc
      421 catcacgctc tcctcgattt gtattatttc aataatctat taattattgg atttattatc
      481 cattattttt atattttatg aatttataca gcaaatgttt tacacaatca acaagattaa
      541 accgtgaaaa ttccacaatc cagccagttt cgtgcaggcc attgatgccc catggcagaa
      601 gctgccacca aacacccacc accgcatgtt tgctgccaat ccctgcctaa caagcttgcc
      661 aaacaagcac ataacgccag caatggcttt ggccataaaa aagcggcctg taaatcagtt
      721 tacaggccgc tttcaaatcc attcaaaccg aatggacatt cgtcacactt gtaccacata
      781 ggttttggcc aagaggtctt gcagggtttg gcggttgcgg ctttccataa acaacatgat
      841 gaagcaagca agagtgggaa ggtagctgac gatttgcaac aacacatcaa tgaccatggc
      901 aacgccgctg ttggcttcca cgccaaacac ggcaacaaaa atacccatca acaccatcat
      961 gatgaccgaa caaatcaaac catacgccac ctcgcgcagc aacacattgt gcacaaaacc
     1021 cgccatgtcg ccgttttcac gcaccacgcg aattttcatc agttttttgc ctatcgattg
     1081 gccgtatttg cacatgaaat acagctgtat gcccccataa atcaggtata acaccatgac
     1141 caaaccgatg ccacccatca tggaagtgaa aaacgattcc atgttttcaa ggtcgtcaag
     1201 accattcttg ttttgcatga tcagcacaac aatcaaaaca atcgccggta tcatcaacac
     1261 aatttcgatg ccgcgattga tgatccatgc cgcgatgcgt tgtgcagccg acgccagatt
     1321 gacttcgtcc tcatcgccac tgctgcccac ttcgacttga gtggagtggt aaatactgtc
     1381 taatggctcc attcgttccc cttcgttaag cctgttttca acctgccaca tctcttaacc
     1441 caaacaccaa cagtgatgca gcgatataaa agattcagtg gcttactaca acaaacaagc
     1501 ggcctgtaaa agtttaca
//
LOCUS       DAOMVK010000559         1517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_24009_length_1517_cov_3.182627, whole genome shotgun sequence.
ACCESSION   DAOMVK010000559 DAOMVK010000000
VERSION     DAOMVK010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24009_length_1517_cov_3.182627"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1517)
                     /locus_tag="PK856_12740"
     CDS             complement(<1..>1517)
                     /locus_tag="PK856_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - exoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HRL03281.1"
                     /translation="ELLEKKGVPVSTEELATVLSIREDEMEFFRRRLGAMVRDGQIHI
                     NRRGLVCVAEKLALVKCRIEAHSDGFGFAVPVDKKDGDTDLVLYERQMRGLMHGDTVT
                     VRPVGVDRRGRREGQVLDIIERANTTVVARLAKEGNIAVAIPEDKRISQSIVLDASSI
                     GDAKDGQVINVELTRYPEKNLPATGKVSEVLGNYADSGMEIEIAVRKHHLPHKFSPAC
                     VKAASKIPDNVKPAERKGRVDLLELPLVTIDGETARDFDDAVFAEKVGRNYRLVVAIA
                     DVSHYVKDGDGIDIDARERGTSVYFPRRVIPMLPENLSNGICSLNPDVERLCMVCDMV
                     ITNMGNVKSYEFYPAVMRSHARLTYTQVWQWIESGEQHDLKPQIDTLYKLYQKLLAKR
                     QQRDAMEFDSIETQMIFNDNGKIDRIEPVVRNEAHRLIEECMLAANVCAAEFLLKNKH
                     PSLYRNHKGPTPEKLETLRTQLTLLGLSLGGGDNPTPKDYAALAEQFTGRPDKEL"
BASE COUNT          339 a          420 c          372 g          386 t
ORIGIN      
        1 caattctttg tctgggcggc ctgtaaactg ttctgccaag gcagcatagt cttttggcgt
       61 agggttatcg ccgccgccca aagacaagcc cagcaaagtc aattgcgtgc gcaaggtttc
      121 caatttttca ggcgtagggc ctttgtgatt gcggtagagc gatgggtgtt tgtttttcag
      181 caaaaattct gcggcgcaca cgttcgcggc caacatgcat tcttcaatca aacggtgggc
      241 ttcgttgcgt accacaggtt caatgcggtc gattttgccg ttgtcattga aaatcatctg
      301 cgtttcgatg ctgtcaaact ccatcgcgtc gcgctgttgg cgtttggcca gcagtttttg
      361 atacagtttg tacaaggtgt cgatttgcgg tttcaaatcg tgttgctcac ccgattctat
      421 ccattgccac acttgggtgt aagtcaggcg ggcatgggag cgcatcaccg cagggtaaaa
      481 ttcgtatgac ttcacgttgc ccatattggt gatgaccatg tcacacacca tgcacaagcg
      541 ctccacatcg ggattgagcg agcaaatgcc attggacaga ttctcaggca acatcggaat
      601 cacacggcgt gggaaataca cactggtgcc acgttcacgg gcatcgatgt cgatgccatc
      661 gccatctttc acatagtggc tgacgtcggc aatcgccacc accaagcggt aattgcggcc
      721 cactttttcg gcaaacacgg catcgtcaaa gtcgcgcgcg gtttcaccat cgatggtgac
      781 caaaggcagc tcgagcaaat ccacacggcc tttgcgttca gcaggtttca cattgtcagg
      841 aatcttactg gccgctttca cgcaagcggg tgagaatttg tgcggtaaat ggtgtttgcg
      901 taccgcaatc tcgatttcca taccgctgtc agcatagtta cccaaaacct cagaaacctt
      961 gcctgtggca ggcagatttt tttcaggata acgggtcaat tccacattga tgacttgacc
     1021 atctttggca tcgcctatac tgctagcatc caaaacgatg ctttgtgaaa tgcgcttgtc
     1081 ttcggggatg gccacggcaa tattgccttc tttggccaaa cgcgccacca ccgtggtgtt
     1141 ggcgcgctca atgatgtcca gcacttggcc ttcacggcga ccgcgcctgt ccacgcctac
     1201 aggccgcaca gtgacggtat cgccatgcat caggccacgc atttggcgct cgtacaacac
     1261 caaatcggta tcgccgtctt ttttatcaac tggtacggca aaaccaaagc cgtcgctgtg
     1321 tgcctcgatg cggcatttga ccaatgccaa cttctcagcc acacacacca agccgcggcg
     1381 gttgatgtgg atttggccat cgcgcaccat cgcacctaag cgacggcgaa aaaattccat
     1441 ttcgtcttca cgtatcgaca acaccgttgc caactcttcc gtgctcacag gcacgccttt
     1501 tttctctaat aattcaa
//
LOCUS       DAOMVK010000560         1508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_24191_length_1508_cov_1.573297, whole genome shotgun sequence.
ACCESSION   DAOMVK010000560 DAOMVK010000000
VERSION     DAOMVK010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24191_length_1508_cov_1.573297"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="PK856_12745"
     CDS             <1..544
                     /locus_tag="PK856_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009119127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3734 domain-containing protein"
                     /protein_id="HRL03282.1"
                     /translation="GDYYWDGGLVSNTPLDDLIENPQSINQLIFQVDLWDARGQVPEN
                     LIGIDERIKDIQYSSKTRYATTVMKKRHTFNHLVKELLQYIPEDQRTAACFKEAEALT
                     SVGFTNVFQLIYRKKAFERGHKDYEFSAHTMYEHWNTGLDDIKATLRQEPWFEMSETG
                     EIFEQHDIHKVRRYSYDNDL"
     gene            801..>1508
                     /locus_tag="PK856_12750"
     CDS             801..>1508
                     /locus_tag="PK856_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRL03283.1"
                     /translation="MDRGQAYQYVCELLKVMLAQKSSDLFITANYPPAMKIDGKMTKI
                     GPQNLSAAHTLALVKSVMTDKQVEEFEATKECNFAISPNGIGRFRVNAFVQQGSAGMV
                     MRVIPTELPTIDGLGAPQILKEVVMSKRGLCILVGGTGSGKSTTLAAMVDYRNEHSYG
                     HIITIEDPVEFVHPHKNCVVMQREVGIDTDNWEIALKNTLRQAPDVILMGEIRDAETM
                     EHAMAFAETGHLCLATLH"
BASE COUNT          461 a          356 c          315 g          376 t
ORIGIN      
        1 tggcgattac tattgggatg gtggcttggt atccaacacg cctttagacg atttgattga
       61 aaacccgcag tctatcaacc aattgatttt ccaagtggac ttatgggatg cgcgcggcca
      121 agtgcctgaa aatttgattg gcattgatga acgcattaaa gacattcaat actcaagtaa
      181 aacccgctac gccaccactg tgatgaaaaa acgccatacc ttcaatcact tggtgaaaga
      241 attgttgcaa tacatacccg aagaccaacg caccgcagct tgctttaaag aggccgaggc
      301 tttgacttca gtaggtttca ccaatgtctt ccaattgatt taccgcaaaa aagcgtttga
      361 acgcggccac aaagactatg aattcagtgc gcacaccatg tatgaacact ggaatactgg
      421 cctagacgac atcaaagcca ctttgcgcca agagccttgg tttgaaatgt cggaaacggg
      481 cgaaatcttt gagcaacacg acattcataa agtgcgccgt tacagctacg ataacgattt
      541 gtaaacacgc attgttttct ataaaagcgg cctgtaaatg tttacaggcc gcttttactc
      601 acatatgcag ccatcacaat tcatatcaaa acccaagtaa cgaattttat ttagttactt
      661 aaatcaaata tatatataat attaaattca tttatcattt taataccgct atcatttcac
      721 caaacaggct tgctttttgc tgtagtcaca gccaatcagt gactctgtca ccaatacaat
      781 atcataagga aataatgcaa atggatcgcg gtcaagccta tcaatatgtg tgcgaattgc
      841 tcaaagtcat gctggcacaa aaaagcagtg atttattcat caccgccaac tacccgccag
      901 ccatgaaaat tgacggcaaa atgaccaaaa taggcccaca aaacctcagc gcagcccata
      961 ccttggcact ggtcaaatcg gtcatgaccg acaaacaagt ggaagaattt gaagccacca
     1021 aagaatgcaa ttttgccatt tcgcccaatg gcatcggtcg cttccgcgtc aatgcctttg
     1081 tacaacaagg ctcagcaggc atggtcatgc gcgtgattcc taccgagtta cccaccattg
     1141 atggcttggg tgcgccgcaa attttaaaag aagtggtgat gagcaaacgt ggcttgtgca
     1201 tcttggtcgg cggtacaggt tcgggtaaat ccaccacctt agctgccatg gtggactatc
     1261 gcaatgaaca ctcatacggc cacatcatca ccattgagga ccctgtggaa tttgtacacc
     1321 cacataaaaa ctgtgtggtg atgcagcgtg aagtcggcat agacaccgac aattgggaaa
     1381 ttgccttaaa aaacaccttg cgccaagcac ctgatgtgat tttgatgggg gaaatccgcg
     1441 atgcagaaac catggaacac gcgatggcat ttgccgaaac aggtcactta tgtttggcaa
     1501 ctttgcat
//
LOCUS       DAOMVK010000561         1501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Vitreoscilla sp. isolate AMR_MDS_5706
            NODE_24346_length_1501_cov_2.616874, whole genome shotgun sequence.
ACCESSION   DAOMVK010000561 DAOMVK010000000
VERSION     DAOMVK010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867927
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vitreoscilla sp. (wastewater metagenome)
  ORGANISM  Vitreoscilla sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Vitreoscilla.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.107
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:37:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,552
            CDSs (total)                      :: 2,522
            Genes (coding)                    :: 2,503
            CDSs (with protein)               :: 2,503
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 4 of 19
            Pseudo Genes (internal stop)      :: 7 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24346_length_1501_cov_2.616874"
                     /isolate="AMR_MDS_5706"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:60"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="PK856_12755"
     CDS             complement(<1..825)
                     /locus_tag="PK856_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HRL03284.1"
                     /translation="MAEHKRLWAMLVLTLALISVYFVWDNTQFIPFVNLLRLKTIAAM
                     VVTAMALGSATLLFHAISNNRIVTPSSLGLEFLYVLAKTSFVFAFGSAALMHISVVWQ
                     FVFGCAILIGFALLLYRMFFKNGRENVFYLLLIGMICMSLFMSINTFLGMLMNPNEFQ
                     IAQDAGFATFNQVDATVLGLAACIVLPLTFYSFSLSRQLDVLGLGRETAINLGIDYER
                     FGKKVMIVVAILMATATSLAGPMFFFGLLILNVTLQWLPTFRHRLLFPAIILVSTMV"
     gene            complement(818..>1501)
                     /locus_tag="PK856_12760"
     CDS             complement(818..>1501)
                     /locus_tag="PK856_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HRL03285.1"
                     /translation="VLLALILLPASTGWLQTSFAAFTTFLGAMCFMAILNTLKHRDTV
                     FVPLVGMMFGNIINSITVFFALQFDLLQNINAWLQGDFSSVLSGRYETLFVSIPALLL
                     AYVFAQRFVLAGMGEDFAMNLGVNYKRTMALGLVLVSIVTAVVIVTVGTIPFLGLIVP
                     NLVALYLGDNLKLTLPHTAVVGALLVLVCDLTGRLVIYPYEVSVSLIMGIIGSAVFLS
                     MLLKRYRHG"
BASE COUNT          469 a          390 c          364 g          278 t
ORIGIN      
        1 caccatggtg ctgaccaaga tgatggcggg aaacagcagg cggtggcgga aggtgggcag
       61 ccattgcaag gtcacgttca aaatcagcag gccaaagaaa aacataggcc ctgcaagcga
      121 ggtggcggtg gccattaata ttgccaccac tatcatcact tttttgccaa agcgttcgta
      181 atcaatgccg aggttgatgg cggtttcacg ccccaaaccc aacacatcca attggcgcga
      241 cagactgaag ctgtaaaacg tgagcggcag cacgatacag gccgccaaac ccaatacggt
      301 ggcatcgact tggttaaacg tggcaaagcc cgcgtcttga gcaatttgaa attcattggg
      361 gttcatcaac atgcccaaga aagtgttgat gctcatgaac aaagacatgc atatcatgcc
      421 gataagcagc aaataaaaca cattctcacg cccgtttttg aagaacatgc ggtaaagcag
      481 caaggcaaag ccgattaaga tggcgcagcc aaacacaaat tgccacacca cgctgatatg
      541 catcaaagcc gcgctgccaa aggcaaacac gaaactggtt ttcgccagca catacaaaaa
      601 ctctaagccc aaagacgaag gcgtgacgat gcggttattg gaaatggcat gaaacagcaa
      661 ggtggcgctg cccaaagcca tggccgtgac caccattgcc gcgatggttt ttaagcgcag
      721 caggttcaca aagggaatga attgggtgtt gtcccagaca aaatacacgc taatcaaggc
      781 caaggtgagc accaacatgg cccacaagcg tttgtgttca gccatgacgg tacctcttca
      841 ataacatgct caagaacacc gcactgccta tgatgcccat gatgaggctg accgatactt
      901 catacgggta gatgaccaag cggcctgtaa ggtcgcacac caacaccaac aaggcgccca
      961 ccaccgcagt gtgtggcaag gtcagtttta aattgtcgcc caaatacaaa gccaccaagt
     1021 tcggtacgat taagcccaaa aacggtatcg tgcccacggt aacgatgacc acggcagtca
     1081 cgatggacac caataccaag cccaaagcca tggtgcgttt gtaattcacg cccaaattca
     1141 tcgcaaagtc ttcgcccatg cccgccagca caaagcgctg tgcaaacaca taagcgagta
     1201 acagcgcagg aatgctgaca aacaaggttt cgtagcgacc gcttaacacg ctggaaaagt
     1261 caccttgcag ccatgcattg atgttttgca gcaaatcaaa ttgcaaggca aaaaacacgg
     1321 tgatggaatt gatgatgttg ccaaacatca tgcccaccaa aggcacaaac acagtgtcgc
     1381 ggtgttttaa ggtattgagt atcgccatga aacacatcgc acccaagaag gtggtgaagg
     1441 cggcaaacga ggtttgcagc caacccgtgc tggcaggcaa taaaattaag gccaataaca
     1501 c
//
